PMC:3333881 JSON TXT

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2_test

Id	Subject	Object	Predicate	Lexical cue
22187158-18927578-77584393	1513-1514	18927578	denotes	1
22187158-19302050-77584394	1888-1889	19302050	denotes	2
22187158-12360211-77584395	1990-1991	12360211	denotes	3
22187158-1829648-77584396	2294-2295	1829648	denotes	4
22187158-7878466-77584397	2343-2344	7878466	denotes	5
22187158-7479976-77584398	2435-2436	7479976	denotes	6
22187158-9660950-77584399	2523-2524	9660950	denotes	7
22187158-12628924-77584400	2525-2526	12628924	denotes	8
22187158-12881425-77584401	2545-2546	12881425	denotes	9
22187158-10521409-77584402	2568-2570	10521409	denotes	10
22187158-14963024-77584403	2571-2573	14963024	denotes	11
22187158-11533489-77584404	2751-2753	11533489	denotes	12
22187158-12456660-77584405	2754-2756	12456660	denotes	13
22187158-8096091-77584406	2947-2949	8096091	denotes	20
22187158-8319912-77584406	2947-2949	8319912	denotes	20
22187158-12424381-77584406	2947-2949	12424381	denotes	20
22187158-15499023-77584406	2947-2949	15499023	denotes	20
22187158-15258597-77584406	2947-2949	15258597	denotes	20
22187158-18676814-77584406	2947-2949	18676814	denotes	20
22187158-11533489-77584407	3085-3087	11533489	denotes	12
22187158-12419806-77584408	3088-3090	12419806	denotes	21
22187158-15152190-77584409	3091-3093	15152190	denotes	22
22187158-7853483-77584410	3359-3361	7853483	denotes	25
22187158-11160127-77584410	3359-3361	11160127	denotes	25
22187158-8764027-77584410	3359-3361	8764027	denotes	25
22187158-18947296-77584411	3444-3446	18947296	denotes	26
22187158-19840555-77584412	3447-3449	19840555	denotes	27
22187158-11160730-77584413	3663-3665	11160730	denotes	28
22187158-18806803-77584414	3666-3668	18806803	denotes	29
22187158-11959143-77584415	3902-3904	11959143	denotes	30
22187158-19287489-77584416	3924-3926	19287489	denotes	31
22187158-2478293-77584417	4226-4228	2478293	denotes	32
22187158-8139045-77584418	4263-4265	8139045	denotes	33
22187158-9169458-77584419	4293-4295	9169458	denotes	34
22187158-7690421-77584420	4464-4466	7690421	denotes	35
22187158-8121496-77584421	4476-4478	8121496	denotes	36
22187158-12055184-77584422	4488-4490	12055184	denotes	37
22187158-15710476-77584423	4501-4503	15710476	denotes	38
22187158-7853496-77584424	4522-4524	7853496	denotes	39
22187158-9491887-77584425	4525-4527	9491887	denotes	40
22187158-9811832-77584426	4587-4589	9811832	denotes	43
22187158-9733868-77584426	4587-4589	9733868	denotes	43
22187158-9733796-77584426	4587-4589	9733796	denotes	43
22187158-10397733-77584427	4717-4719	10397733	denotes	44
22187158-8946838-77584428	4741-4743	8946838	denotes	45
22187158-9789057-77584429	4770-4772	9789057	denotes	46
22187158-8113688-77584430	4882-4884	8113688	denotes	47
22187158-8676466-77584431	5007-5009	8676466	denotes	48
22187158-18329615-77584432	5122-5124	18329615	denotes	49
22187158-8096091-77584433	5351-5353	8096091	denotes	15
22187158-12424381-77584434	5354-5356	12424381	denotes	17
22187158-17942396-77584435	5464-5466	17942396	denotes	50
22187158-21664225-77584436	5467-5469	21664225	denotes	51
22187158-17942396-77584437	6077-6079	17942396	denotes	50
22187158-14532125-77584438	6670-6672	14532125	denotes	52
22187158-19782003-77584439	6887-6889	19782003	denotes	53
22187158-17942396-77584440	9315-9317	17942396	denotes	50
22187158-8537390-77584441	9467-9469	8537390	denotes	54
22187158-15613239-77584442	10882-10884	15613239	denotes	55
22187158-17942396-77584443	11133-11135	17942396	denotes	50
22187158-17942396-77584444	11366-11368	17942396	denotes	50
22187158-12424381-77584445	18935-18937	12424381	denotes	17
22187158-15499023-77584446	18938-18940	15499023	denotes	18
22187158-17942396-77584447	21882-21884	17942396	denotes	50
22187158-21664225-77584448	21885-21887	21664225	denotes	51
22187158-17942396-77584449	23094-23096	17942396	denotes	50
22187158-21664225-77584450	23097-23099	21664225	denotes	51
22187158-15203102-77584451	33111-33113	15203102	denotes	56
22187158-10751368-77584452	33229-33231	10751368	denotes	57
22187158-19596987-77584453	33232-33234	19596987	denotes	58
22187158-8570619-77584454	33297-33299	8570619	denotes	59
22187158-11602715-77584455	33300-33302	11602715	denotes	60
22187158-10751368-77584456	33321-33323	10751368	denotes	57
22187158-10964543-77584457	33324-33326	10964543	denotes	61
22187158-20403075-77584458	33327-33329	20403075	denotes	62
22187158-18329615-77584459	39726-39728	18329615	denotes	49
22187158-1583734-77584460	39729-39731	1583734	denotes	63
22187158-10544088-77584461	39732-39734	10544088	denotes	64
22187158-17942396-77584462	39789-39791	17942396	denotes	50
22187158-21664225-77584463	39792-39794	21664225	denotes	51
22187158-11959143-77584464	40179-40181	11959143	denotes	30
22187158-19287489-77584465	40182-40184	19287489	denotes	31
22187158-17942396-77584466	41753-41755	17942396	denotes	50
22187158-21664225-77584467	41756-41758	21664225	denotes	51
22187158-15536134-77584468	43408-43410	15536134	denotes	65
22187158-15536134-77584469	43838-43840	15536134	denotes	65
22187158-2257304-77584470	44787-44789	2257304	denotes	66
22187158-1930778-77584471	44790-44792	1930778	denotes	67
22187158-2114424-77584472	44802-44804	2114424	denotes	68
22187158-10751368-77584473	44822-44824	10751368	denotes	62
22187158-19596987-77584473	44822-44824	19596987	denotes	62
22187158-8570619-77584473	44822-44824	8570619	denotes	62
22187158-11602715-77584473	44822-44824	11602715	denotes	62
22187158-10964543-77584473	44822-44824	10964543	denotes	62
22187158-20403075-77584473	44822-44824	20403075	denotes	62
22187158-8634452-77584474	44828-44830	8634452	denotes	71
22187158-8623908-77584474	44828-44830	8623908	denotes	71
22187158-11287580-77584474	44828-44830	11287580	denotes	71
22187158-8139045-77584475	45015-45017	8139045	denotes	33
22187158-12055184-77584476	45018-45020	12055184	denotes	37
22187158-8113688-77584477	45021-45023	8113688	denotes	47
22187158-1727476-77584478	45460-45462	1727476	denotes	72
22187158-16280076-77584479	45463-45465	16280076	denotes	73
22187158-18947296-77584480	45569-45571	18947296	denotes	26
22187158-10602409-77584481	45605-45607	10602409	denotes	74
22187158-16697675-77584482	45608-45610	16697675	denotes	75
22187158-19149660-77584483	45962-45964	19149660	denotes	76
22187158-19426715-77584484	45965-45967	19426715	denotes	77

pmc-enju-pas

Id	Subject	Object	Predicate	Lexical cue
T142	0-5	JJ	denotes	Human
T143	6-22	NN	denotes	immunodeficiency
T144	23-30	NN	denotes	virus-1
T145	31-34	NN	denotes	Tat
T146	35-44	VB	denotes	activates
T147	45-50	NN	denotes	NF-κB
T148	51-54	IN	denotes	via
T149	55-63	JJ	denotes	physical
T150	64-75	NN	denotes	interaction
T151	76-80	IN	denotes	with
T152	81-86	NN	denotes	IκB-α
T153	87-90	CC	denotes	and
T154	91-94	NN	denotes	p65
T155	106-113	JJ	denotes	Nuclear
T156	114-120	NN	denotes	factor
T157	121-122	-LRB-	denotes	(
T158	122-124	NN	denotes	NF
T159	124-125	-RRB-	denotes	)
T160	125-128	NN	denotes	-κB
T161	129-131	VB	denotes	is
T162	132-133	DT	denotes	a
T163	134-140	NN	denotes	master
T164	141-150	NN	denotes	regulator
T165	151-153	IN	denotes	of
T166	154-170	JJ	denotes	pro-inflammatory
T167	171-176	NN	denotes	genes
T168	177-180	CC	denotes	and
T169	181-183	VB	denotes	is
T170	184-195	VB	denotes	upregulated
T171	196-198	IN	denotes	in
T172	199-204	JJ	denotes	human
T173	205-221	NN	denotes	immunodeficiency
T174	222-227	NN	denotes	virus
T175	228-229	CD	denotes	1
T176	230-231	-LRB-	denotes	(
T177	231-236	NN	denotes	HIV-1
T178	236-237	-RRB-	denotes	)
T179	238-247	NN	denotes	infection
T180	249-259	NN	denotes	Mechanisms
T181	260-270	VB	denotes	underlying
T182	271-274	DT	denotes	the
T183	275-280	NN	denotes	NF-κB
T184	281-293	NN	denotes	deregulation
T185	294-296	IN	denotes	by
T186	297-302	NN	denotes	HIV-1
T187	303-306	VB	denotes	are
T188	307-315	JJ	denotes	relevant
T189	316-319	IN	denotes	for
T190	320-326	JJ	denotes	immune
T191	327-338	NN	denotes	dysfunction
T192	339-341	IN	denotes	in
T193	342-346	NN	denotes	AIDS
T194	348-350	PRP	denotes	We
T195	351-357	VB	denotes	report
T196	358-362	IN	denotes	that
T197	363-365	IN	denotes	in
T198	366-372	JJ	denotes	single
T199	373-378	JJ	denotes	round
T200	379-384	NN	denotes	HIV-1
T201	385-394	NN	denotes	infection
T202	394-395	-COMMA-	denotes	,
T203	396-398	CC	denotes	or
T204	399-411	JJ	denotes	single-pulse
T205	412-415	NN	denotes	PMA
T206	416-427	NN	denotes	stimulation
T207	427-428	-COMMA-	denotes	,
T208	429-432	DT	denotes	the
T209	433-438	NN	denotes	HIV-1
T210	439-442	NN	denotes	Tat
T211	443-457	NN	denotes	transactivator
T212	458-467	VB	denotes	activated
T213	468-473	NN	denotes	NF-κB
T214	474-476	IN	denotes	by
T215	477-486	VB	denotes	hijacking
T216	487-490	DT	denotes	the
T217	491-500	NN	denotes	inhibitor
T218	501-506	NN	denotes	IκB-α
T219	507-510	CC	denotes	and
T220	511-513	IN	denotes	by
T221	514-524	VB	denotes	preventing
T222	525-528	DT	denotes	the
T223	529-538	NN	denotes	repressor
T224	539-546	NN	denotes	binding
T225	547-549	TO	denotes	to
T226	550-553	DT	denotes	the
T227	554-559	NN	denotes	NF-κB
T228	560-567	NN	denotes	complex
T229	569-577	RB	denotes	Moreover
T230	577-578	-COMMA-	denotes	,
T231	579-582	NN	denotes	Tat
T232	583-593	VB	denotes	associated
T233	594-598	IN	denotes	with
T234	599-602	DT	denotes	the
T235	603-606	NN	denotes	p65
T236	607-614	NN	denotes	subunit
T237	615-617	IN	denotes	of
T238	618-623	NN	denotes	NF-κB
T239	624-627	CC	denotes	and
T240	628-637	VB	denotes	increased
T241	638-641	DT	denotes	the
T242	642-645	NN	denotes	p65
T243	646-657	JJ	denotes	DNA-binding
T244	658-666	NN	denotes	affinity
T245	667-670	CC	denotes	and
T246	671-686	JJ	denotes	transcriptional
T247	687-695	NN	denotes	activity
T248	697-700	DT	denotes	The
T249	701-710	NN	denotes	arginine-
T250	711-714	CC	denotes	and
T251	715-728	JJ	denotes	cysteine-rich
T252	729-736	NN	denotes	domains
T253	737-739	IN	denotes	of
T254	740-743	NN	denotes	Tat
T255	744-748	VB	denotes	were
T256	749-757	VB	denotes	required
T257	758-761	IN	denotes	for
T258	762-767	NN	denotes	IκB-α
T259	768-771	CC	denotes	and
T260	772-775	NN	denotes	p65
T261	776-787	NN	denotes	association
T262	787-788	-COMMA-	denotes	,
T263	789-801	RB	denotes	respectively
T264	801-802	-COMMA-	denotes	,
T265	803-806	CC	denotes	and
T266	807-810	IN	denotes	for
T267	811-821	VB	denotes	sustaining
T268	822-825	DT	denotes	the
T269	826-831	NN	denotes	NF-κB
T270	832-840	NN	denotes	activity
T271	842-847	IN	denotes	Among
T272	848-850	DT	denotes	an
T273	851-856	NN	denotes	array
T274	857-859	IN	denotes	of
T275	860-876	JJ	denotes	NF-κB-responsive
T276	877-882	NN	denotes	genes
T277	882-883	-COMMA-	denotes	,
T278	884-887	NN	denotes	Tat
T279	888-894	RB	denotes	mostly
T280	895-904	VB	denotes	activated
T281	905-908	DT	denotes	the
T282	909-915	NN	denotes	MIP-1α
T283	916-926	NN	denotes	expression
T284	927-929	IN	denotes	in
T285	930-931	DT	denotes	a
T286	932-945	JJ	denotes	p65-dependent
T287	946-952	NN	denotes	manner
T288	952-953	-COMMA-	denotes	,
T289	954-957	CC	denotes	and
T290	958-963	VB	denotes	bound
T291	964-966	TO	denotes	to
T292	967-970	DT	denotes	the
T293	971-977	NN	denotes	MIP-1α
T294	978-983	NN	denotes	NF-κB
T295	984-992	NN	denotes	enhancer
T296	993-997	RB	denotes	thus
T297	998-1007	VB	denotes	promoting
T298	1008-1011	DT	denotes	the
T299	1012-1023	NN	denotes	recruitment
T300	1024-1026	IN	denotes	of
T301	1027-1030	NN	denotes	p65
T302	1031-1035	IN	denotes	with
T303	1036-1048	NN	denotes	displacement
T304	1049-1051	IN	denotes	of
T305	1052-1057	NN	denotes	IκB-α
T306	1057-1058	-COLON-	denotes	;
T307	1059-1066	JJ	denotes	similar
T308	1067-1075	NN	denotes	findings
T309	1076-1080	VB	denotes	were
T310	1081-1089	VB	denotes	obtained
T311	1090-1093	IN	denotes	for
T312	1094-1097	DT	denotes	the
T313	1098-1114	JJ	denotes	NF-κB-responsive
T314	1115-1120	NN	denotes	genes
T315	1121-1125	NN	denotes	CSF3
T316	1125-1126	-COMMA-	denotes	,
T317	1127-1130	NN	denotes	LTA
T318	1130-1131	-COMMA-	denotes	,
T319	1132-1138	NN	denotes	NFKBIA
T320	1139-1142	CC	denotes	and
T321	1143-1147	NN	denotes	TLR2
T322	1149-1152	PRP-DOLLAR-	denotes	Our
T323	1153-1160	NN	denotes	results
T324	1161-1168	VB	denotes	support
T325	1169-1170	DT	denotes	a
T326	1171-1176	JJ	denotes	novel
T327	1177-1186	NN	denotes	mechanism
T328	1187-1189	IN	denotes	of
T329	1190-1195	NN	denotes	NF-κB
T330	1196-1206	NN	denotes	activation
T331	1207-1210	IN	denotes	via
T332	1211-1219	JJ	denotes	physical
T333	1220-1231	NN	denotes	interaction
T334	1232-1234	IN	denotes	of
T335	1235-1238	NN	denotes	Tat
T336	1239-1243	IN	denotes	with
T337	1244-1249	NN	denotes	IκB-α
T338	1250-1253	CC	denotes	and
T339	1254-1257	NN	denotes	p65
T340	1257-1258	-COMMA-	denotes	,
T341	1259-1262	CC	denotes	and
T342	1263-1266	MD	denotes	may
T343	1267-1277	VB	denotes	contribute
T344	1278-1280	TO	denotes	to
T345	1281-1288	JJ	denotes	further
T346	1289-1297	NN	denotes	insights
T347	1298-1302	IN	denotes	into
T348	1303-1306	DT	denotes	the
T349	1307-1319	NN	denotes	deregulation
T350	1320-1322	IN	denotes	of
T351	1323-1326	DT	denotes	the
T352	1327-1339	JJ	denotes	inflammatory
T353	1340-1348	NN	denotes	response
T354	1349-1351	IN	denotes	by
T355	1352-1357	NN	denotes	HIV-1
T1454	1373-1380	JJ	denotes	Nuclear
T1455	1381-1387	NN	denotes	factor
T1456	1388-1389	-LRB-	denotes	(
T1457	1389-1391	NN	denotes	NF
T1458	1391-1392	-RRB-	denotes	)
T1459	1392-1395	NN	denotes	-κB
T1460	1396-1409	NN	denotes	transcription
T1461	1410-1417	NN	denotes	factors
T1462	1418-1426	VB	denotes	regulate
T1463	1427-1430	DT	denotes	the
T1464	1431-1444	NN	denotes	transcription
T1465	1445-1447	IN	denotes	of
T1466	1448-1453	NN	denotes	genes
T1467	1454-1458	WDT	denotes	that
T1468	1459-1462	VB	denotes	are
T1469	1463-1471	VB	denotes	involved
T1470	1472-1474	IN	denotes	in
T1471	1475-1478	DT	denotes	the
T1472	1479-1485	JJ	denotes	immune
T1473	1486-1489	CC	denotes	and
T1474	1490-1502	JJ	denotes	inflammatory
T1475	1503-1511	NN	denotes	response
T1476	1512-1513	-LRB-	denotes	(
T1477	1513-1514	CD	denotes	1
T1478	1514-1515	-RRB-	denotes	)
T1479	1517-1520	DT	denotes	The
T1480	1521-1526	NN	denotes	NF-κB
T1481	1527-1533	NN	denotes	family
T1482	1534-1542	VB	denotes	includes
T1483	1543-1551	NN	denotes	RelA/p65
T1484	1551-1552	-COMMA-	denotes	,
T1485	1553-1558	NN	denotes	c-Rel
T1486	1558-1559	-COMMA-	denotes	,
T1487	1560-1564	NN	denotes	RelB
T1488	1564-1565	-COMMA-	denotes	,
T1489	1566-1569	NN	denotes	p50
T1490	1570-1573	CC	denotes	and
T1491	1574-1577	NN	denotes	p52
T1492	1578-1582	WDT	denotes	that
T1493	1583-1588	VB	denotes	share
T1494	1589-1590	DT	denotes	a
T1495	1591-1597	RB	denotes	highly
T1496	1598-1607	VB	denotes	conserved
T1497	1608-1617	JJ	denotes	300-amino
T1498	1618-1622	NN	denotes	acid
T1499	1623-1626	NN	denotes	Rel
T1500	1627-1635	NN	denotes	homology
T1501	1636-1642	NN	denotes	domain
T1502	1643-1644	-LRB-	denotes	(
T1503	1644-1647	NN	denotes	RHD
T1504	1647-1648	-RRB-	denotes	)
T1505	1649-1652	IN	denotes	for
T1506	1653-1658	JJ	denotes	homo-
T1507	1659-1661	CC	denotes	or
T1508	1662-1681	NN	denotes	hetero-dimerization
T1509	1682-1685	CC	denotes	and
T1510	1686-1697	NN	denotes	DNA-binding
T1511	1699-1702	DT	denotes	The
T1512	1703-1718	JJ	denotes	transcriptional
T1513	1719-1727	NN	denotes	activity
T1514	1728-1730	IN	denotes	of
T1515	1731-1734	DT	denotes	the
T1516	1735-1740	NN	denotes	NF-κB
T1517	1741-1748	NN	denotes	complex
T1518	1749-1756	VB	denotes	depends
T1519	1757-1759	IN	denotes	on
T1520	1760-1765	NN	denotes	dimer
T1521	1766-1777	NN	denotes	composition
T1522	1778-1783	IN	denotes	since
T1523	1784-1794	JJ	denotes	C-terminal
T1524	1795-1804	JJ	denotes	unrelated
T1525	1805-1820	JJ	denotes	transcriptional
T1526	1821-1831	NN	denotes	activation
T1527	1832-1839	NN	denotes	domains
T1528	1840-1843	VB	denotes	are
T1529	1844-1851	JJ	denotes	present
T1530	1852-1863	RB	denotes	exclusively
T1531	1864-1866	IN	denotes	in
T1532	1867-1870	NN	denotes	p65
T1533	1870-1871	-COMMA-	denotes	,
T1534	1872-1876	NN	denotes	RelB
T1535	1877-1880	CC	denotes	and
T1536	1881-1886	NN	denotes	c-Rel
T1537	1887-1888	-LRB-	denotes	(
T1538	1888-1889	CD	denotes	2
T1539	1889-1890	-RRB-	denotes	)
T1540	1892-1902	NN	denotes	Inhibitors
T1541	1903-1905	IN	denotes	of
T1542	1906-1911	NN	denotes	NF-κB
T1543	1912-1913	-LRB-	denotes	(
T1544	1913-1916	NN	denotes	IκB
T1545	1916-1917	-RRB-	denotes	)
T1546	1918-1927	VB	denotes	associate
T1547	1928-1932	IN	denotes	with
T1548	1933-1936	DT	denotes	the
T1549	1937-1942	NN	denotes	NF-κB
T1550	1943-1950	NN	denotes	complex
T1551	1951-1954	CC	denotes	and
T1552	1955-1964	VB	denotes	interfere
T1553	1965-1969	IN	denotes	with
T1554	1970-1973	PRP-DOLLAR-	denotes	its
T1555	1974-1981	NN	denotes	binding
T1556	1982-1984	TO	denotes	to
T1557	1985-1988	NN	denotes	DNA
T1558	1989-1990	-LRB-	denotes	(
T1559	1990-1991	CD	denotes	3
T1560	1991-1992	-RRB-	denotes	)
T1561	1994-1996	IN	denotes	In
T1562	1997-2000	DT	denotes	the
T1563	2001-2010	JJ	denotes	canonical
T1564	2011-2018	NN	denotes	pathway
T1565	2019-2021	IN	denotes	of
T1566	2022-2027	NN	denotes	NF-κB
T1567	2028-2038	NN	denotes	activation
T1568	2038-2039	-COMMA-	denotes	,
T1569	2040-2043	DT	denotes	the
T1570	2044-2053	VB	denotes	activated
T1571	2054-2057	NN	denotes	IκB
T1572	2058-2064	NN	denotes	kinase
T1573	2065-2066	-LRB-	denotes	(
T1574	2066-2069	NN	denotes	IKK
T1575	2069-2070	-RRB-	denotes	)
T1576	2071-2085	VB	denotes	phosphorylates
T1577	2086-2089	NN	denotes	IκB
T1578	2090-2092	IN	denotes	at
T1579	2093-2101	JJ	denotes	specific
T1580	2102-2108	NN	denotes	serine
T1581	2109-2117	NN	denotes	residues
T1582	2118-2122	WDT	denotes	that
T1583	2123-2129	VB	denotes	target
T1584	2130-2133	DT	denotes	the
T1585	2134-2141	NN	denotes	protein
T1586	2142-2144	TO	denotes	to
T1587	2145-2159	NN	denotes	ubiquitination
T1588	2160-2163	CC	denotes	and
T1589	2164-2175	JJ	denotes	proteasomal
T1590	2176-2187	NN	denotes	degradation
T1591	2187-2188	-COMMA-	denotes	,
T1592	2189-2194	WDT	denotes	which
T1593	2195-2203	VB	denotes	releases
T1594	2204-2207	DT	denotes	the
T1595	2208-2218	JJ	denotes	functional
T1596	2219-2224	NN	denotes	NF-κB
T1597	2225-2232	NN	denotes	complex
T1598	2233-2235	IN	denotes	in
T1599	2236-2239	DT	denotes	the
T1600	2240-2247	NN	denotes	nucleus
T1601	2249-2254	NN	denotes	IκB-α
T1602	2254-2255	-COMMA-	denotes	,
T1603	2256-2259	DT	denotes	the
T1604	2260-2264	RB	denotes	most
T1605	2265-2273	JJ	denotes	abundant
T1606	2274-2283	NN	denotes	inhibitor
T1607	2284-2286	IN	denotes	of
T1608	2287-2292	NN	denotes	NF-κB
T1609	2293-2294	-LRB-	denotes	(
T1610	2294-2295	CD	denotes	4
T1611	2295-2296	-RRB-	denotes	)
T1612	2296-2297	-COMMA-	denotes	,
T1613	2298-2300	VB	denotes	is
T1614	2301-2315	VB	denotes	phosphorylated
T1615	2316-2318	IN	denotes	by
T1616	2319-2322	NN	denotes	IKK
T1617	2323-2325	IN	denotes	at
T1618	2326-2331	NN	denotes	Ser32
T1619	2332-2335	CC	denotes	and
T1620	2336-2341	NN	denotes	Ser36
T1621	2342-2343	-LRB-	denotes	(
T1622	2343-2344	CD	denotes	5
T1623	2344-2345	-RRB-	denotes	)
T1624	2345-2346	-COMMA-	denotes	,
T1625	2347-2350	CC	denotes	and
T1626	2351-2363	RB	denotes	subsequently
T1627	2364-2377	VB	denotes	ubiquitylated
T1628	2378-2380	IN	denotes	at
T1629	2381-2386	NN	denotes	Lys21
T1630	2387-2390	CC	denotes	and
T1631	2391-2396	NN	denotes	Lys22
T1632	2397-2399	TO	denotes	to
T1633	2400-2402	VB	denotes	be
T1634	2403-2411	VB	denotes	degraded
T1635	2412-2414	IN	denotes	by
T1636	2415-2418	DT	denotes	the
T1637	2419-2422	NN	denotes	26S
T1638	2423-2433	NN	denotes	proteasome
T1639	2434-2435	-LRB-	denotes	(
T1640	2435-2436	CD	denotes	6
T1641	2436-2437	-RRB-	denotes	)
T1642	2439-2442	DT	denotes	The
T1643	2443-2448	NN	denotes	NF-κB
T1644	2449-2457	NN	denotes	activity
T1645	2458-2460	VB	denotes	is
T1646	2461-2469	VB	denotes	enhanced
T1647	2470-2472	IN	denotes	by
T1648	2473-2488	NN	denotes	phosphorylation
T1649	2489-2491	IN	denotes	of
T1650	2492-2495	NN	denotes	p65
T1651	2496-2498	IN	denotes	at
T1652	2499-2505	NN	denotes	Ser276
T1653	2506-2508	IN	denotes	by
T1654	2509-2512	NN	denotes	PKA
T1655	2513-2516	CC	denotes	and
T1656	2517-2521	NN	denotes	MSK1
T1657	2522-2523	-LRB-	denotes	(
T1658	2523-2526	NN	denotes	7,8
T1659	2526-2527	-RRB-	denotes	)
T1660	2527-2528	-COMMA-	denotes	,
T1661	2529-2535	NN	denotes	Ser311
T1662	2536-2538	IN	denotes	by
T1663	2539-2543	NN	denotes	PKCζ
T1664	2544-2545	-LRB-	denotes	(
T1665	2545-2546	CD	denotes	9
T1666	2546-2547	-RRB-	denotes	)
T1667	2548-2551	CC	denotes	and
T1668	2552-2558	NN	denotes	Ser536
T1669	2559-2561	IN	denotes	by
T1670	2562-2566	NN	denotes	IKKα
T1671	2567-2568	-LRB-	denotes	(
T1672	2568-2573	CD	denotes	10,11
T1673	2573-2574	-RRB-	denotes	)
T1674	2576-2587	NN	denotes	Acetylation
T1675	2588-2590	IN	denotes	of
T1676	2591-2594	NN	denotes	p65
T1677	2595-2597	IN	denotes	at
T1678	2598-2604	NN	denotes	Lys218
T1679	2605-2608	CC	denotes	and
T1680	2609-2615	NN	denotes	Lys221
T1681	2616-2625	VB	denotes	increases
T1682	2626-2629	DT	denotes	the
T1683	2630-2633	NN	denotes	DNA
T1684	2634-2641	NN	denotes	binding
T1685	2642-2645	CC	denotes	and
T1686	2646-2653	VB	denotes	impairs
T1687	2654-2657	DT	denotes	the
T1688	2658-2669	NN	denotes	association
T1689	2670-2674	IN	denotes	with
T1690	2675-2680	NN	denotes	IκB-α
T1691	2680-2681	-COMMA-	denotes	,
T1692	2682-2685	CC	denotes	and
T1693	2686-2697	NN	denotes	acetylation
T1694	2698-2700	IN	denotes	at
T1695	2701-2707	NN	denotes	Lys310
T1696	2708-2716	VB	denotes	enhances
T1697	2717-2720	DT	denotes	the
T1698	2721-2724	NN	denotes	p65
T1699	2725-2740	JJ	denotes	transcriptional
T1700	2741-2749	NN	denotes	activity
T1701	2750-2751	-LRB-	denotes	(
T1702	2751-2756	CD	denotes	12,13
T1703	2756-2757	-RRB-	denotes	)
T1704	2759-2774	NN	denotes	Post-activation
T1705	2775-2779	VB	denotes	turn
T1706	2780-2783	RP	denotes	off
T1707	2784-2786	IN	denotes	of
T1708	2787-2792	NN	denotes	NF-κB
T1709	2793-2795	VB	denotes	is
T1710	2796-2805	VB	denotes	regulated
T1711	2806-2808	IN	denotes	by
T1712	2809-2817	JJ	denotes	negative
T1713	2818-2826	NN	denotes	feedback
T1714	2827-2831	NN	denotes	loop
T1715	2832-2839	IN	denotes	through
T1716	2840-2850	NN	denotes	inhibitors
T1717	2851-2856	IN	denotes	under
T1718	2857-2860	DT	denotes	the
T1719	2861-2876	JJ	denotes	transcriptional
T1720	2877-2884	NN	denotes	control
T1721	2885-2887	IN	denotes	of
T1722	2888-2893	NN	denotes	NF-κB
T1723	2893-2894	-COMMA-	denotes	,
T1724	2895-2899	JJ	denotes	such
T1725	2900-2902	IN	denotes	as
T1726	2903-2908	JJ	denotes	IκB-α
T1727	2909-2912	CC	denotes	and
T1728	2913-2930	JJ	denotes	ubiquitin-editing
T1729	2931-2938	NN	denotes	protein
T1730	2939-2942	NN	denotes	A20
T1731	2943-2944	-LRB-	denotes	(
T1732	2944-2949	CD	denotes	14–20
T1733	2949-2950	-RRB-	denotes	)
T1734	2952-2965	NN	denotes	Deacetylation
T1735	2966-2968	IN	denotes	of
T1736	2969-2972	NN	denotes	p65
T1737	2973-2975	IN	denotes	by
T1738	2976-2983	NN	denotes	histone
T1739	2984-2997	NN	denotes	deacetylase-3
T1740	2998-3000	CC	denotes	or
T1741	3001-3006	NN	denotes	SIRT1
T1742	3006-3007	-COMMA-	denotes	,
T1743	3008-3010	CC	denotes	or
T1744	3011-3022	NN	denotes	acetylation
T1745	3023-3025	IN	denotes	of
T1746	3026-3029	NN	denotes	p65
T1747	3030-3032	IN	denotes	at
T1748	3033-3039	NN	denotes	Lys122
T1749	3040-3043	CC	denotes	and
T1750	3044-3050	NN	denotes	Lys123
T1751	3051-3064	VB	denotes	down-regulate
T1752	3065-3068	DT	denotes	the
T1753	3069-3074	NN	denotes	NF-κB
T1754	3075-3083	NN	denotes	activity
T1755	3084-3085	-LRB-	denotes	(
T1756	3085-3093	CD	denotes	12,21,22
T1757	3093-3094	-RRB-	denotes	)
T1758	3096-3106	JJ	denotes	Persistent
T1759	3107-3117	NN	denotes	activation
T1760	3118-3120	IN	denotes	of
T1761	3121-3126	NN	denotes	NF-κB
T1762	3127-3133	VB	denotes	occurs
T1763	3134-3136	IN	denotes	in
T1764	3137-3142	JJ	denotes	human
T1765	3143-3159	NN	denotes	immunodeficiency
T1766	3160-3167	NN	denotes	virus-1
T1767	3168-3169	-LRB-	denotes	(
T1768	3169-3174	NN	denotes	HIV-1
T1769	3174-3175	-RRB-	denotes	)
T1770	3175-3184	JJ	denotes	-infected
T1771	3185-3194	NN	denotes	monocytes
T1772	3194-3195	-COMMA-	denotes	,
T1773	3196-3207	NN	denotes	macrophages
T1774	3208-3211	CC	denotes	and
T1775	3212-3221	NN	denotes	microglia
T1776	3221-3222	-COMMA-	denotes	,
T1777	3223-3226	CC	denotes	and
T1778	3227-3235	VB	denotes	enhances
T1779	3236-3239	DT	denotes	the
T1780	3240-3250	NN	denotes	expression
T1781	3251-3253	IN	denotes	of
T1782	3254-3270	JJ	denotes	NF-κB-responsive
T1783	3271-3276	NN	denotes	genes
T1784	3276-3277	-COMMA-	denotes	,
T1785	3278-3287	VB	denotes	including
T1786	3288-3304	JJ	denotes	pro-inflammatory
T1787	3305-3314	NN	denotes	cytokines
T1788	3314-3315	-COMMA-	denotes	,
T1789	3316-3320	NN	denotes	cell
T1790	3321-3329	NN	denotes	adhesion
T1791	3330-3339	NN	denotes	molecules
T1792	3340-3343	CC	denotes	and
T1793	3344-3354	NN	denotes	chemokines
T1794	3355-3356	-LRB-	denotes	(
T1795	3356-3361	CD	denotes	23–25
T1796	3361-3362	-RRB-	denotes	)
T1797	3364-3371	JJ	denotes	Chronic
T1798	3372-3384	NN	denotes	inflammation
T1799	3385-3387	VB	denotes	is
T1800	3388-3389	DT	denotes	a
T1801	3390-3395	JJ	denotes	major
T1802	3396-3401	NN	denotes	cause
T1803	3402-3404	IN	denotes	of
T1804	3405-3411	JJ	denotes	immune
T1805	3412-3415	CC	denotes	and
T1806	3416-3422	NN	denotes	neuron
T1807	3423-3434	NN	denotes	dysfunction
T1808	3435-3437	IN	denotes	in
T1809	3438-3442	NN	denotes	AIDS
T1810	3443-3444	-LRB-	denotes	(
T1811	3444-3449	CD	denotes	26,27
T1812	3449-3450	-RRB-	denotes	)
T1813	3452-3464	RB	denotes	Consistently
T1814	3464-3465	-COMMA-	denotes	,
T1815	3466-3475	JJ	denotes	non-human
T1816	3476-3483	JJ	denotes	primate
T1817	3484-3489	NN	denotes	hosts
T1818	3490-3493	IN	denotes	for
T1819	3494-3500	JJ	denotes	simian
T1820	3501-3517	NN	denotes	immunodeficiency
T1821	3518-3523	NN	denotes	virus
T1822	3523-3524	-COMMA-	denotes	,
T1823	3525-3529	JJ	denotes	such
T1824	3530-3532	IN	denotes	as
T1825	3533-3540	JJ	denotes	African
T1826	3541-3546	JJ	denotes	green
T1827	3547-3554	NN	denotes	monkeys
T1828	3555-3558	CC	denotes	and
T1829	3559-3564	JJ	denotes	sooty
T1830	3565-3573	NN	denotes	mangabey
T1831	3573-3574	-COMMA-	denotes	,
T1832	3575-3579	VB	denotes	lack
T1833	3580-3588	JJ	denotes	aberrant
T1834	3589-3595	JJ	denotes	immune
T1835	3596-3606	NN	denotes	activation
T1836	3607-3610	CC	denotes	and
T1837	3611-3613	VB	denotes	do
T1838	3614-3617	RB	denotes	not
T1839	3618-3625	VB	denotes	develop
T1840	3626-3630	NN	denotes	AIDS
T1841	3631-3638	IN	denotes	despite
T1842	3639-3643	JJ	denotes	high
T1843	3644-3649	NN	denotes	virus
T1844	3650-3661	NN	denotes	replication
T1845	3662-3663	-LRB-	denotes	(
T1846	3663-3668	CD	denotes	28,29
T1847	3668-3669	-RRB-	denotes	)
T1848	3671-3675	RB	denotes	Thus
T1849	3675-3676	-COMMA-	denotes	,
T1850	3677-3690	VB	denotes	understanding
T1851	3691-3694	DT	denotes	the
T1852	3695-3705	NN	denotes	mechanisms
T1853	3706-3708	IN	denotes	of
T1854	3709-3714	NN	denotes	NF-κB
T1855	3715-3727	NN	denotes	deregulation
T1856	3728-3730	IN	denotes	by
T1857	3731-3736	NN	denotes	HIV-1
T1858	3737-3740	MD	denotes	may
T1859	3741-3748	VB	denotes	provide
T1860	3749-3756	JJ	denotes	further
T1861	3757-3765	NN	denotes	insights
T1862	3766-3770	IN	denotes	into
T1863	3771-3775	NN	denotes	AIDS
T1864	3776-3788	NN	denotes	pathogenesis
T1865	3790-3792	IN	denotes	In
T1866	3793-3798	NN	denotes	HIV-1
T1867	3799-3804	NN	denotes	entry
T1868	3804-3805	-COMMA-	denotes	,
T1869	3806-3809	DT	denotes	the
T1870	3810-3817	NN	denotes	binding
T1871	3818-3820	IN	denotes	of
T1872	3821-3824	DT	denotes	the
T1873	3825-3830	NN	denotes	gp120
T1874	3831-3836	JJ	denotes	viral
T1875	3837-3845	NN	denotes	envelope
T1876	3846-3848	TO	denotes	to
T1877	3849-3852	NN	denotes	CD4
T1878	3853-3860	VB	denotes	induces
T1879	3861-3864	DT	denotes	the
T1880	3865-3870	NN	denotes	NF-κB
T1881	3871-3879	NN	denotes	activity
T1882	3880-3882	IN	denotes	by
T1883	3883-3893	NN	denotes	activation
T1884	3894-3896	IN	denotes	of
T1885	3897-3900	NN	denotes	IKK
T1886	3901-3902	-LRB-	denotes	(
T1887	3902-3904	CD	denotes	30
T1888	3904-3905	-RRB-	denotes	)
T1889	3906-3909	CC	denotes	and
T1890	3910-3920	NN	denotes	procaspase
T1891	3921-3922	CD	denotes	8
T1892	3923-3924	-LRB-	denotes	(
T1893	3924-3926	CD	denotes	31
T1894	3926-3927	-RRB-	denotes	)
T1895	3929-3938	VB	denotes	Following
T1896	3939-3944	JJ	denotes	viral
T1897	3945-3956	NN	denotes	integration
T1898	3956-3957	-COMMA-	denotes	,
T1899	3958-3961	DT	denotes	the
T1900	3962-3967	JJ	denotes	early
T1901	3968-3975	VB	denotes	encoded
T1902	3976-3981	NN	denotes	HIV-1
T1903	3982-3985	NN	denotes	Tat
T1904	3986-3993	NN	denotes	protein
T1905	3994-4003	VB	denotes	interacts
T1906	4004-4008	IN	denotes	with
T1907	4009-4012	DT	denotes	the
T1908	4013-4018	NN	denotes	HIV-1
T1909	4019-4022	NN	denotes	RNA
T1910	4023-4026	CC	denotes	and
T1911	4027-4031	NN	denotes	host
T1912	4032-4036	NN	denotes	cell
T1913	4037-4044	NN	denotes	factors
T1914	4045-4047	TO	denotes	to
T1915	4048-4055	VB	denotes	sustain
T1916	4056-4059	DT	denotes	the
T1917	4060-4065	JJ	denotes	viral
T1918	4066-4077	NN	denotes	replication
T1919	4079-4082	NN	denotes	Tat
T1920	4083-4088	VB	denotes	binds
T1921	4089-4091	TO	denotes	to
T1922	4092-4095	NN	denotes	RNA
T1923	4096-4105	JJ	denotes	stem-loop
T1924	4106-4116	NN	denotes	structures
T1925	4117-4126	VB	denotes	generated
T1926	4127-4129	IN	denotes	by
T1927	4130-4133	DT	denotes	the
T1928	4134-4136	NN	denotes	5′
T1929	4137-4140	NN	denotes	end
T1930	4141-4143	IN	denotes	of
T1931	4144-4150	NN	denotes	target
T1932	4151-4162	NN	denotes	transcripts
T1933	4162-4163	-COMMA-	denotes	,
T1934	4164-4173	VB	denotes	including
T1935	4174-4177	DT	denotes	the
T1936	4178-4183	NN	denotes	HIV-1
T1937	4184-4210	JJ	denotes	transactivation-responsive
T1938	4211-4218	NN	denotes	element
T1939	4219-4220	-LRB-	denotes	(
T1940	4220-4223	NN	denotes	TAR
T1941	4223-4224	-RRB-	denotes	)
T1942	4225-4226	-LRB-	denotes	(
T1943	4226-4228	CD	denotes	32
T1944	4228-4229	-RRB-	denotes	)
T1945	4229-4230	-COMMA-	denotes	,
T1946	4231-4236	NN	denotes	tumor
T1947	4237-4245	NN	denotes	necrosis
T1948	4246-4252	NN	denotes	factor
T1949	4253-4254	NN	denotes	β
T1950	4255-4256	-LRB-	denotes	(
T1951	4256-4260	NN	denotes	TNFβ
T1952	4260-4261	-RRB-	denotes	)
T1953	4262-4263	-LRB-	denotes	(
T1954	4263-4265	CD	denotes	33
T1955	4265-4266	-RRB-	denotes	)
T1956	4267-4270	CC	denotes	and
T1957	4271-4284	NN	denotes	interleukin-6
T1958	4285-4286	-LRB-	denotes	(
T1959	4286-4290	NN	denotes	IL-6
T1960	4290-4291	-RRB-	denotes	)
T1961	4292-4293	-LRB-	denotes	(
T1962	4293-4295	CD	denotes	34
T1963	4295-4296	-RRB-	denotes	)
T1964	4297-4299	TO	denotes	to
T1965	4300-4308	VB	denotes	activate
T1966	4309-4313	NN	denotes	gene
T1967	4314-4327	NN	denotes	transcription
T1968	4329-4335	RB	denotes	Indeed
T1969	4335-4336	-COMMA-	denotes	,
T1970	4337-4340	NN	denotes	Tat
T1971	4341-4349	VB	denotes	promotes
T1972	4350-4353	DT	denotes	the
T1973	4354-4369	JJ	denotes	transcriptional
T1974	4370-4380	NN	denotes	initiation
T1975	4381-4384	CC	denotes	and
T1976	4385-4395	NN	denotes	elongation
T1977	4396-4398	IN	denotes	by
T1978	4399-4410	VB	denotes	interacting
T1979	4411-4415	IN	denotes	with
T1980	4416-4427	VB	denotes	transacting
T1981	4428-4435	NN	denotes	factors
T1982	4436-4439	CC	denotes	and
T1983	4440-4449	NN	denotes	cofactors
T1984	4449-4450	-COMMA-	denotes	,
T1985	4451-4455	JJ	denotes	such
T1986	4456-4458	IN	denotes	as
T1987	4459-4462	NN	denotes	Sp1
T1988	4463-4464	-LRB-	denotes	(
T1989	4464-4466	CD	denotes	35
T1990	4466-4467	-RRB-	denotes	)
T1991	4467-4468	-COMMA-	denotes	,
T1992	4469-4474	NN	denotes	TFIID
T1993	4475-4476	-LRB-	denotes	(
T1994	4476-4478	CD	denotes	36
T1995	4478-4479	-RRB-	denotes	)
T1996	4479-4480	-COMMA-	denotes	,
T1997	4481-4486	NN	denotes	E2F-4
T1998	4487-4488	-LRB-	denotes	(
T1999	4488-4490	CD	denotes	37
T2000	4490-4491	-RRB-	denotes	)
T2001	4491-4492	-COMMA-	denotes	,
T2002	4493-4499	NN	denotes	C/EBPβ
T2003	4500-4501	-LRB-	denotes	(
T2004	4501-4503	CD	denotes	38
T2005	4503-4504	-RRB-	denotes	)
T2006	4504-4505	-COMMA-	denotes	,
T2007	4506-4512	NN	denotes	cyclin
T2008	4513-4520	NN	denotes	T1/CDK9
T2009	4521-4522	-LRB-	denotes	(
T2010	4522-4527	CD	denotes	39,40
T2011	4527-4528	-RRB-	denotes	)
T2012	4529-4532	CC	denotes	and
T2013	4533-4536	DT	denotes	the
T2014	4537-4544	NN	denotes	histone
T2015	4545-4563	NN	denotes	acetyltransferases
T2016	4564-4572	NN	denotes	p300/CBP
T2017	4573-4576	CC	denotes	and
T2018	4577-4582	NN	denotes	P/CAF
T2019	4583-4584	-LRB-	denotes	(
T2020	4584-4589	CD	denotes	41–43
T2021	4589-4590	-RRB-	denotes	)
T2022	4592-4596	WRB	denotes	When
T2023	4597-4605	VB	denotes	released
T2024	4606-4610	IN	denotes	from
T2025	4611-4625	JJ	denotes	HIV-1-infected
T2026	4626-4631	NN	denotes	cells
T2027	4631-4632	-COMMA-	denotes	,
T2028	4633-4636	NN	denotes	Tat
T2029	4637-4648	VB	denotes	deregulates
T2030	4649-4652	DT	denotes	the
T2031	4653-4657	NN	denotes	cell
T2032	4658-4667	NN	denotes	signaling
T2033	4668-4670	IN	denotes	by
T2034	4671-4678	VB	denotes	binding
T2035	4679-4681	TO	denotes	to
T2036	4682-4686	NN	denotes	cell
T2037	4687-4696	NN	denotes	receptors
T2038	4696-4697	-COMMA-	denotes	,
T2039	4698-4702	JJ	denotes	such
T2040	4703-4705	IN	denotes	as
T2041	4706-4715	NN	denotes	integrins
T2042	4716-4717	-LRB-	denotes	(
T2043	4717-4719	CD	denotes	44
T2044	4719-4720	-RRB-	denotes	)
T2045	4720-4721	-COMMA-	denotes	,
T2046	4722-4730	NN	denotes	Flk1/KDR
T2047	4731-4739	NN	denotes	receptor
T2048	4740-4741	-LRB-	denotes	(
T2049	4741-4743	CD	denotes	45
T2050	4743-4744	-RRB-	denotes	)
T2051	4745-4748	CC	denotes	and
T2052	4749-4758	NN	denotes	chemokine
T2053	4759-4768	NN	denotes	receptors
T2054	4769-4770	-LRB-	denotes	(
T2055	4770-4772	CD	denotes	46
T2056	4772-4773	-RRB-	denotes	)
T2057	4775-4777	PRP	denotes	We
T2058	4778-4783	RB	denotes	first
T2059	4784-4792	VB	denotes	reported
T2060	4793-4797	IN	denotes	that
T2061	4798-4803	NN	denotes	NF-κB
T2062	4804-4807	VB	denotes	was
T2063	4808-4822	RB	denotes	constitutively
T2064	4823-4829	JJ	denotes	active
T2065	4830-4832	IN	denotes	in
T2066	4833-4839	NN	denotes	Jurkat
T2067	4840-4845	NN	denotes	cells
T2068	4846-4850	WDT	denotes	that
T2069	4851-4857	RB	denotes	stably
T2070	4858-4867	VB	denotes	expressed
T2071	4868-4871	DT	denotes	the
T2072	4872-4875	NN	denotes	Tat
T2073	4876-4880	NN	denotes	gene
T2074	4881-4882	-LRB-	denotes	(
T2075	4882-4884	CD	denotes	47
T2076	4884-4885	-RRB-	denotes	)
T2077	4887-4896	VB	denotes	Following
T2078	4897-4901	NN	denotes	gene
T2079	4902-4914	NN	denotes	transfection
T2080	4915-4917	CC	denotes	or
T2081	4918-4925	NN	denotes	protein
T2082	4926-4938	NN	denotes	transduction
T2083	4938-4939	-COMMA-	denotes	,
T2084	4940-4943	NN	denotes	Tat
T2085	4944-4951	VB	denotes	induced
T2086	4952-4955	DT	denotes	the
T2087	4956-4959	NN	denotes	IKK
T2088	4960-4968	NN	denotes	activity
T2089	4969-4972	CC	denotes	and
T2090	4973-4984	JJ	denotes	proteasomal
T2091	4985-4996	NN	denotes	degradation
T2092	4997-4999	IN	denotes	of
T2093	5000-5005	NN	denotes	IκB-α
T2094	5006-5007	-LRB-	denotes	(
T2095	5007-5009	CD	denotes	48
T2096	5009-5010	-RRB-	denotes	)
T2097	5010-5011	-COMMA-	denotes	,
T2098	5012-5015	CC	denotes	and
T2099	5016-5025	VB	denotes	increased
T2100	5026-5029	DT	denotes	the
T2101	5030-5033	NN	denotes	p65
T2102	5034-5049	JJ	denotes	transcriptional
T2103	5050-5058	NN	denotes	activity
T2104	5059-5061	IN	denotes	by
T2105	5062-5072	VB	denotes	inhibiting
T2106	5073-5076	DT	denotes	the
T2107	5077-5092	JJ	denotes	SIRT-1-mediated
T2108	5093-5106	NN	denotes	deacetylation
T2109	5107-5109	IN	denotes	of
T2110	5110-5113	NN	denotes	p65
T2111	5114-5120	NN	denotes	Lys310
T2112	5121-5122	-LRB-	denotes	(
T2113	5122-5124	CD	denotes	49
T2114	5124-5125	-RRB-	denotes	)
T2115	5127-5132	DT	denotes	These
T2116	5133-5141	NN	denotes	findings
T2117	5142-5151	VB	denotes	suggested
T2118	5152-5156	IN	denotes	that
T2119	5157-5160	NN	denotes	Tat
T2120	5161-5170	VB	denotes	modulates
T2121	5171-5178	JJ	denotes	crucial
T2122	5179-5186	NN	denotes	enzymes
T2123	5187-5195	VB	denotes	involved
T2124	5196-5198	IN	denotes	in
T2125	5199-5204	NN	denotes	NF-κB
T2126	5205-5214	NN	denotes	signaling
T2127	5214-5215	-COLON-	denotes	;
T2128	5216-5223	RB	denotes	however
T2129	5223-5224	-COMMA-	denotes	,
T2130	5225-5227	PRP	denotes	it
T2131	5228-5231	VB	denotes	was
T2132	5232-5239	JJ	denotes	unclear
T2133	5240-5243	WRB	denotes	how
T2134	5244-5247	NN	denotes	Tat
T2135	5248-5253	MD	denotes	could
T2136	5254-5261	VB	denotes	subvert
T2137	5262-5265	DT	denotes	the
T2138	5266-5274	JJ	denotes	negative
T2139	5275-5283	NN	denotes	feedback
T2140	5284-5286	IN	denotes	of
T2141	5287-5292	NN	denotes	NF-κB
T2142	5292-5293	-COMMA-	denotes	,
T2143	5294-5299	WDT	denotes	which
T2144	5300-5302	VB	denotes	is
T2145	5303-5309	RB	denotes	mainly
T2146	5310-5319	JJ	denotes	dependent
T2147	5320-5322	IN	denotes	on
T2148	5323-5325	FW	denotes	de
T2149	5326-5330	FW	denotes	novo
T2150	5331-5340	NN	denotes	synthesis
T2151	5341-5343	IN	denotes	of
T2152	5344-5349	NN	denotes	IκB-α
T2153	5350-5351	-LRB-	denotes	(
T2154	5351-5356	CD	denotes	15,17
T2155	5356-5357	-RRB-	denotes	)
T2156	5359-5361	PRP	denotes	We
T2157	5362-5372	RB	denotes	previously
T2158	5373-5378	VB	denotes	found
T2159	5379-5383	IN	denotes	that
T2160	5384-5389	NN	denotes	IκB-α
T2161	5390-5395	VB	denotes	binds
T2162	5396-5398	TO	denotes	to
T2163	5399-5402	NN	denotes	Tat
T2164	5403-5406	CC	denotes	and
T2165	5407-5415	VB	denotes	promotes
T2166	5416-5419	DT	denotes	the
T2167	5420-5427	JJ	denotes	nuclear
T2168	5428-5434	NN	denotes	export
T2169	5435-5437	IN	denotes	of
T2170	5438-5441	DT	denotes	the
T2171	5442-5447	JJ	denotes	viral
T2172	5448-5462	NN	denotes	transactivator
T2173	5463-5464	-LRB-	denotes	(
T2174	5464-5469	CD	denotes	50,51
T2175	5469-5470	-RRB-	denotes	)
T2176	5472-5474	IN	denotes	In
T2177	5475-5479	DT	denotes	this
T2178	5480-5485	NN	denotes	study
T2179	5485-5486	-COMMA-	denotes	,
T2180	5487-5489	PRP	denotes	we
T2181	5490-5496	VB	denotes	report
T2182	5497-5501	IN	denotes	that
T2183	5502-5505	NN	denotes	Tat
T2184	5506-5517	VB	denotes	counteracts
T2185	5518-5521	DT	denotes	the
T2186	5522-5537	JJ	denotes	post-activation
T2187	5538-5542	NN	denotes	turn
T2188	5543-5546	RP	denotes	off
T2189	5547-5549	IN	denotes	of
T2190	5550-5555	NN	denotes	NF-κB
T2191	5556-5563	IN	denotes	through
T2192	5564-5570	JJ	denotes	direct
T2193	5571-5582	NN	denotes	interaction
T2194	5583-5587	IN	denotes	with
T2195	5588-5593	NN	denotes	IκB-α
T2196	5594-5597	CC	denotes	and
T2197	5598-5601	NN	denotes	p65
T2198	5601-5602	-COMMA-	denotes	,
T2199	5603-5608	WDT	denotes	which
T2200	5609-5617	VB	denotes	enhances
T2201	5618-5621	DT	denotes	the
T2202	5622-5625	NN	denotes	DNA
T2203	5626-5633	NN	denotes	binding
T2204	5634-5637	CC	denotes	and
T2205	5638-5653	JJ	denotes	transcriptional
T2206	5654-5662	NN	denotes	activity
T2207	5663-5665	IN	denotes	of
T2208	5666-5669	DT	denotes	the
T2209	5670-5675	NN	denotes	NF-κB
T2210	5676-5683	NN	denotes	complex
T2211	5685-5688	DT	denotes	The
T2212	5689-5692	JJ	denotes	new
T2213	5693-5702	NN	denotes	mechanism
T2214	5703-5705	IN	denotes	of
T2215	5706-5711	NN	denotes	NF-κB
T2216	5712-5724	NN	denotes	deregulation
T2217	5725-5729	RB	denotes	here
T2218	5730-5739	VB	denotes	described
T2219	5740-5743	MD	denotes	may
T2220	5744-5751	VB	denotes	provide
T2221	5752-5759	JJ	denotes	further
T2222	5760-5768	NN	denotes	insights
T2223	5769-5773	IN	denotes	into
T2224	5774-5777	DT	denotes	the
T2225	5778-5785	JJ	denotes	chronic
T2226	5786-5792	JJ	denotes	immune
T2227	5793-5803	NN	denotes	activation
T2228	5804-5806	IN	denotes	of
T2229	5807-5812	NN	denotes	HIV-1
T2230	5813-5822	NN	denotes	infection
T4413	5848-5856	NN	denotes	Plasmids
T4414	5857-5860	DT	denotes	The
T4415	5861-5869	NN	denotes	plasmids
T4416	5870-5886	NN	denotes	pcDNA-3xHA-IκB-α
T4417	5886-5887	-COMMA-	denotes	,
T4418	5888-5903	NN	denotes	p3xFLAG-CMV-Tat
T4419	5903-5904	-COMMA-	denotes	,
T4420	5905-5920	NN	denotes	p3xFLAG-CMV-Tat
T4421	5921-5922	NN	denotes	C
T4422	5922-5923	-LRB-	denotes	(
T4423	5923-5931	CD	denotes	22,25,27
T4424	5931-5932	-RRB-	denotes	)
T4425	5932-5933	NN	denotes	A
T4426	5933-5934	-COMMA-	denotes	,
T4427	5935-5950	NN	denotes	p3xFLAG-CMV-Tat
T4428	5951-5952	NN	denotes	R
T4429	5952-5953	-LRB-	denotes	(
T4430	5953-5970	CD	denotes	49,52,53,55,56,57
T4431	5970-5971	-RRB-	denotes	)
T4432	5971-5972	NN	denotes	A
T4433	5972-5973	-COMMA-	denotes	,
T4434	5974-5985	NN	denotes	pGEX-2T-Tat
T4435	5985-5986	-COMMA-	denotes	,
T4436	5987-5998	NN	denotes	pGEX-2T-Tat
T4437	5999-6000	NN	denotes	C
T4438	6000-6001	-LRB-	denotes	(
T4439	6001-6009	CD	denotes	22,25,27
T4440	6009-6010	-RRB-	denotes	)
T4441	6010-6011	NN	denotes	A
T4442	6012-6015	CC	denotes	and
T4443	6016-6027	NN	denotes	pGEX-2T-Tat
T4444	6028-6029	NN	denotes	R
T4445	6029-6030	-LRB-	denotes	(
T4446	6030-6047	CD	denotes	49,52,53,55,56,57
T4447	6047-6048	-RRB-	denotes	)
T4448	6048-6049	NN	denotes	A
T4449	6050-6054	VB	denotes	were
T4450	6055-6065	RB	denotes	previously
T4451	6066-6075	VB	denotes	described
T4452	6076-6077	-LRB-	denotes	(
T4453	6077-6079	CD	denotes	50
T4454	6079-6080	-RRB-	denotes	)
T4455	6082-6085	DT	denotes	The
T4456	6086-6094	NN	denotes	plasmids
T4457	6095-6110	NN	denotes	pNL4-3.Luc.R-E-
T4458	6111-6114	CC	denotes	and
T4459	6115-6124	NN	denotes	pHXB2-env
T4460	6125-6129	VB	denotes	were
T4461	6130-6138	VB	denotes	obtained
T4462	6139-6143	IN	denotes	from
T4463	6144-6147	DT	denotes	the
T4464	6148-6152	NNP	denotes	AIDS
T4465	6153-6161	NNP	denotes	Research
T4466	6162-6163	CC	denotes	&
T4467	6164-6173	NNP	denotes	Reference
T4468	6174-6181	NNP	denotes	Reagent
T4469	6182-6189	NNP	denotes	Program
T4470	6189-6190	-COMMA-	denotes	,
T4471	6191-6199	NNP	denotes	Division
T4472	6200-6202	IN	denotes	of
T4473	6203-6207	NNP	denotes	AIDS
T4474	6207-6208	-COMMA-	denotes	,
T4475	6209-6214	NNP	denotes	NIAID
T4476	6214-6215	-COMMA-	denotes	,
T4477	6216-6219	NNP	denotes	NIH
T4478	6219-6220	-COMMA-	denotes	,
T4479	6221-6224	NNP	denotes	USA
T4480	6224-6225	-COLON-	denotes	;
T4481	6226-6232	NN	denotes	pκBluc
T4482	6233-6236	CC	denotes	and
T4483	6237-6246	NN	denotes	pSV-β-Gal
T4484	6247-6251	VB	denotes	were
T4485	6252-6261	VB	denotes	purchased
T4486	6262-6266	IN	denotes	from
T4487	6267-6274	NNP	denotes	Promega
T4488	6275-6276	-LRB-	denotes	(
T4489	6276-6283	NNP	denotes	Madison
T4490	6283-6284	-COMMA-	denotes	,
T4491	6285-6287	NNP	denotes	WI
T4492	6287-6288	-COMMA-	denotes	,
T4493	6289-6292	NNP	denotes	USA
T4494	6292-6293	-RRB-	denotes	)
T4495	6295-6298	DT	denotes	The
T4496	6299-6307	NN	denotes	plasmids
T4497	6308-6324	NN	denotes	pRc/CMV-3xHA-p65
T4498	6324-6325	-COMMA-	denotes	,
T4499	6326-6344	NN	denotes	pRc/CMV-3xHA-p65ΔC
T4500	6344-6345	-LRB-	denotes	(
T4501	6345-6350	CD	denotes	1–318
T4502	6350-6351	-RRB-	denotes	)
T4503	6351-6352	-COMMA-	denotes	,
T4504	6353-6371	NN	denotes	pRc/CMV-3xHA-p65ΔN
T4505	6371-6372	-LRB-	denotes	(
T4506	6372-6379	CD	denotes	122–551
T4507	6379-6380	-RRB-	denotes	)
T4508	6380-6381	-COMMA-	denotes	,
T4509	6382-6397	NN	denotes	p3xFLAG-CMV-Tat
T4510	6398-6399	NN	denotes	T
T4511	6399-6400	-COMMA-	denotes	,
T4512	6400-6401	NN	denotes	N
T4513	6401-6402	-LRB-	denotes	(
T4514	6402-6407	CD	denotes	23,24
T4515	6407-6408	-RRB-	denotes	)
T4516	6408-6409	NN	denotes	A
T4517	6409-6410	-COMMA-	denotes	,
T4518	6411-6426	NN	denotes	p3xFLAG-CMV-Tat
T4519	6427-6428	NN	denotes	K
T4520	6428-6429	-LRB-	denotes	(
T4521	6429-6434	CD	denotes	50,51
T4522	6434-6435	-RRB-	denotes	)
T4523	6435-6436	NN	denotes	A
T4524	6436-6437	-COMMA-	denotes	,
T4525	6438-6449	NN	denotes	pGEX-2T-Tat
T4526	6450-6451	NN	denotes	T
T4527	6451-6452	-COMMA-	denotes	,
T4528	6452-6453	NN	denotes	N
T4529	6453-6454	-LRB-	denotes	(
T4530	6454-6459	CD	denotes	23,24
T4531	6459-6460	-RRB-	denotes	)
T4532	6460-6461	NN	denotes	A
T4533	6461-6462	-COMMA-	denotes	,
T4534	6463-6474	NN	denotes	pGEX-2T-Tat
T4535	6475-6476	NN	denotes	K
T4536	6476-6477	-LRB-	denotes	(
T4537	6477-6482	CD	denotes	50,51
T4538	6482-6483	-RRB-	denotes	)
T4539	6483-6484	NN	denotes	A
T4540	6485-6488	CC	denotes	and
T4541	6489-6509	NN	denotes	pNL4-3.FLAG-Tat.R-E-
T4542	6510-6514	VB	denotes	were
T4543	6515-6524	VB	denotes	generated
T4544	6525-6527	IN	denotes	as
T4545	6528-6537	VB	denotes	described
T4546	6538-6540	IN	denotes	in
T4547	6541-6554	NNP	denotes	Supplementary
T4548	6555-6559	NNP	denotes	Data
T4915	6562-6567	NN	denotes	Cells
T4916	6567-6568	-COMMA-	denotes	,
T4917	6569-6581	NN	denotes	transfection
T4918	6581-6582	-COMMA-	denotes	,
T4919	6583-6593	NN	denotes	treatments
T4920	6594-6597	CC	denotes	and
T4921	6598-6608	NN	denotes	luciferase
T4922	6609-6614	NN	denotes	assay
T4923	6615-6619	NN	denotes	HeLa
T4924	6619-6620	-COMMA-	denotes	,
T4925	6621-6633	NN	denotes	p50−/−p65−/−
T4926	6634-6639	NN	denotes	mouse
T4927	6640-6649	JJ	denotes	embryonic
T4928	6650-6661	NN	denotes	fibroblasts
T4929	6662-6663	-LRB-	denotes	(
T4930	6663-6667	NN	denotes	MEFs
T4931	6667-6668	-RRB-	denotes	)
T4932	6669-6670	-LRB-	denotes	(
T4933	6670-6672	CD	denotes	52
T4934	6672-6673	-RRB-	denotes	)
T4935	6674-6677	CC	denotes	and
T4936	6678-6682	NN	denotes	293T
T4937	6683-6688	NN	denotes	cells
T4938	6689-6693	VB	denotes	were
T4939	6694-6702	VB	denotes	cultured
T4940	6703-6705	IN	denotes	in
T4941	6706-6714	NNP	denotes	Dulbecco
T4942	6714-6716	POS	denotes	's
T4943	6717-6725	VB	denotes	modified
T4944	6726-6731	NNP	denotes	Eagle
T4945	6731-6733	POS	denotes	's
T4946	6734-6740	NN	denotes	medium
T4947	6740-6741	-COLON-	denotes	;
T4948	6742-6748	NN	denotes	Jurkat
T4949	6748-6749	-COMMA-	denotes	,
T4950	6750-6754	NN	denotes	U937
T4951	6755-6760	NN	denotes	cells
T4952	6761-6764	CC	denotes	and
T4953	6765-6770	JJ	denotes	human
T4954	6771-6781	JJ	denotes	peripheral
T4955	6782-6787	NN	denotes	blood
T4956	6788-6799	JJ	denotes	mononuclear
T4957	6800-6805	NN	denotes	cells
T4958	6806-6807	-LRB-	denotes	(
T4959	6807-6812	NN	denotes	PBMCs
T4960	6812-6813	-RRB-	denotes	)
T4961	6814-6818	VB	denotes	were
T4962	6819-6827	VB	denotes	cultured
T4963	6828-6830	IN	denotes	in
T4964	6831-6835	NN	denotes	RPMI
T4965	6836-6840	CD	denotes	1640
T4966	6842-6847	NN	denotes	PBMCs
T4967	6848-6852	VB	denotes	were
T4968	6853-6861	VB	denotes	isolated
T4969	6862-6864	IN	denotes	as
T4970	6865-6875	RB	denotes	previously
T4971	6876-6885	VB	denotes	described
T4972	6886-6887	-LRB-	denotes	(
T4973	6887-6889	CD	denotes	53
T4974	6889-6890	-RRB-	denotes	)
T4975	6892-6897	NN	denotes	Media
T4976	6898-6902	VB	denotes	were
T4977	6903-6915	VB	denotes	supplemented
T4978	6916-6920	IN	denotes	with
T4979	6921-6923	CD	denotes	10
T4980	6923-6924	NN	denotes	%
T4981	6925-6941	JJ	denotes	heat-inactivated
T4982	6942-6947	JJ	denotes	fetal
T4983	6948-6952	NN	denotes	calf
T4984	6953-6958	NN	denotes	serum
T4985	6959-6962	CC	denotes	and
T4986	6963-6967	NN	denotes	2 mM
T4987	6968-6979	NN	denotes	l-glutamine
T4988	6980-6981	-LRB-	denotes	(
T4989	6981-6986	NNP	denotes	Lonza
T4990	6987-6994	NNP	denotes	Cologne
T4991	6995-6997	NNP	denotes	AG
T4992	6997-6998	-COMMA-	denotes	,
T4993	6999-7006	NNP	denotes	Germany
T4994	7006-7007	-RRB-	denotes	)
T4995	7009-7013	NN	denotes	HeLa
T4996	7013-7014	-COMMA-	denotes	,
T4997	7015-7027	NN	denotes	p50−/−p65−/−
T4998	7028-7032	NN	denotes	MEFs
T4999	7033-7036	CC	denotes	and
T5000	7037-7041	NN	denotes	293T
T5001	7042-7046	VB	denotes	were
T5002	7047-7058	VB	denotes	transfected
T5003	7059-7063	IN	denotes	with
T5004	7064-7067	NN	denotes	DNA
T5005	7068-7070	IN	denotes	by
T5006	7071-7076	VB	denotes	using
T5007	7077-7083	NN	denotes	FuGENE
T5008	7084-7086	NN	denotes	HD
T5009	7087-7088	-LRB-	denotes	(
T5010	7088-7093	NNP	denotes	Roche
T5011	7094-7104	NNP	denotes	Diagnostic
T5012	7105-7109	NNP	denotes	GmbH
T5013	7109-7110	-COMMA-	denotes	,
T5014	7111-7119	NNP	denotes	Mannheim
T5015	7119-7120	-COMMA-	denotes	,
T5016	7121-7128	NNP	denotes	Germany
T5017	7128-7129	-RRB-	denotes	)
T5018	7129-7130	-COMMA-	denotes	,
T5019	7131-7140	VB	denotes	according
T5020	7141-7143	TO	denotes	to
T5021	7144-7147	DT	denotes	the
T5022	7148-7160	NN	denotes	manufacturer
T5023	7160-7162	POS	denotes	's
T5024	7163-7171	NN	denotes	protocol
T5025	7171-7172	-COLON-	denotes	;
T5026	7173-7178	JJ	denotes	total
T5027	7179-7182	NN	denotes	DNA
T5028	7183-7190	NN	denotes	amounts
T5029	7191-7195	VB	denotes	were
T5030	7196-7205	VB	denotes	equalized
T5031	7206-7208	IN	denotes	by
T5032	7209-7221	NN	denotes	transfection
T5033	7222-7224	IN	denotes	of
T5034	7225-7232	NN	denotes	pRc/CMV
T5035	7233-7238	NN	denotes	empty
T5036	7239-7245	NN	denotes	vector
T5037	7246-7247	-LRB-	denotes	(
T5038	7247-7257	NN	denotes	Invitrogen
T5039	7257-7258	-COMMA-	denotes	,
T5040	7259-7267	NNP	denotes	Carlsbad
T5041	7267-7268	-COMMA-	denotes	,
T5042	7269-7271	NNP	denotes	CA
T5043	7271-7272	-COMMA-	denotes	,
T5044	7273-7276	NNP	denotes	USA
T5045	7276-7277	-RRB-	denotes	)
T5046	7279-7282	IN	denotes	For
T5047	7283-7300	NN	denotes	pulse-stimulation
T5048	7300-7301	-COMMA-	denotes	,
T5049	7302-7306	NN	denotes	HeLa
T5050	7307-7312	NN	denotes	cells
T5051	7313-7317	VB	denotes	were
T5052	7318-7325	VB	denotes	treated
T5053	7326-7330	IN	denotes	with
T5054	7331-7338	NN	denotes	phorbol
T5055	7339-7351	NN	denotes	12-myristate
T5056	7352-7362	NN	denotes	13-acetate
T5057	7363-7364	-LRB-	denotes	(
T5058	7364-7367	NN	denotes	PMA
T5059	7367-7368	-COLON-	denotes	;
T5060	7369-7382	JJ	denotes	Sigma-Aldrich
T5061	7382-7383	-COMMA-	denotes	,
T5062	7384-7386	NNP	denotes	St
T5063	7387-7392	NNP	denotes	Louis
T5064	7392-7393	-COMMA-	denotes	,
T5065	7394-7396	NN	denotes	MO
T5066	7396-7397	-COMMA-	denotes	,
T5067	7398-7401	NNP	denotes	USA
T5068	7401-7402	-RRB-	denotes	)
T5069	7403-7404	-LRB-	denotes	(
T5070	7404-7412	NN	denotes	20 ng/ml
T5071	7412-7413	-RRB-	denotes	)
T5072	7414-7417	IN	denotes	for
T5073	7418-7423	NN	denotes	5 min
T5074	7423-7424	-COMMA-	denotes	,
T5075	7425-7427	CC	denotes	or
T5076	7428-7433	NN	denotes	tumor
T5077	7434-7442	NN	denotes	necrosis
T5078	7443-7451	NN	denotes	factor-α
T5079	7452-7453	-LRB-	denotes	(
T5080	7453-7458	NN	denotes	TNF-α
T5081	7458-7459	-COLON-	denotes	;
T5082	7460-7473	JJ	denotes	Sigma-Aldrich
T5083	7473-7474	-RRB-	denotes	)
T5084	7475-7476	-LRB-	denotes	(
T5085	7476-7484	NN	denotes	20 ng/ml
T5086	7484-7485	-RRB-	denotes	)
T5087	7486-7489	IN	denotes	for
T5088	7490-7496	NN	denotes	30 min
T5089	7496-7497	-COMMA-	denotes	,
T5090	7498-7504	VB	denotes	washed
T5091	7505-7510	RB	denotes	twice
T5092	7511-7513	IN	denotes	in
T5093	7514-7522	JJ	denotes	complete
T5094	7523-7530	NN	denotes	culture
T5095	7531-7537	NN	denotes	medium
T5096	7538-7541	CC	denotes	and
T5097	7542-7546	RB	denotes	then
T5098	7547-7555	VB	denotes	returned
T5099	7556-7558	TO	denotes	to
T5100	7559-7566	NN	denotes	culture
T5101	7568-7571	IN	denotes	For
T5102	7572-7582	NN	denotes	luciferase
T5103	7583-7589	NN	denotes	assays
T5104	7589-7590	-COMMA-	denotes	,
T5105	7591-7600	NN	denotes	pSV-β-Gal
T5106	7601-7604	VB	denotes	was
T5107	7605-7619	VB	denotes	co-transfected
T5108	7620-7624	IN	denotes	with
T5109	7625-7631	NN	denotes	pκBluc
T5110	7632-7634	TO	denotes	to
T5111	7635-7642	VB	denotes	monitor
T5112	7643-7646	DT	denotes	the
T5113	7647-7659	NN	denotes	transfection
T5114	7660-7670	NN	denotes	efficiency
T5115	7672-7688	JJ	denotes	Forty-eight-hour
T5116	7689-7706	JJ	denotes	post-transfection
T5117	7706-7707	-COMMA-	denotes	,
T5118	7708-7713	NN	denotes	cells
T5119	7714-7718	VB	denotes	were
T5120	7719-7724	VB	denotes	lysed
T5121	7725-7727	IN	denotes	in
T5122	7728-7733	NN	denotes	lysis
T5123	7734-7740	NN	denotes	buffer
T5124	7741-7743	IN	denotes	of
T5125	7744-7748	NNP	denotes	Dual
T5126	7749-7754	NNP	denotes	Light
T5127	7755-7765	NNP	denotes	Luciferase
T5128	7766-7772	NNP	denotes	System
T5129	7773-7774	-LRB-	denotes	(
T5130	7774-7780	NNP	denotes	Tropix
T5131	7780-7781	-COMMA-	denotes	,
T5132	7782-7789	NNP	denotes	Bedford
T5133	7789-7790	-COMMA-	denotes	,
T5134	7791-7793	NNP	denotes	MA
T5135	7793-7794	-COMMA-	denotes	,
T5136	7795-7798	NNP	denotes	USA
T5137	7798-7799	-RRB-	denotes	)
T5138	7800-7803	CC	denotes	and
T5139	7804-7807	DT	denotes	the
T5140	7808-7818	NN	denotes	luciferase
T5141	7819-7822	CC	denotes	and
T5142	7823-7838	NN	denotes	β-galactosidase
T5143	7839-7849	NN	denotes	activities
T5144	7850-7854	VB	denotes	were
T5145	7855-7864	VB	denotes	evaluated
T5146	7865-7867	IN	denotes	by
T5147	7868-7873	VB	denotes	using
T5148	7874-7878	NNP	denotes	Dual
T5149	7879-7884	NNP	denotes	Light
T5150	7885-7895	NNP	denotes	Luciferase
T5151	7896-7902	NNP	denotes	System
T5152	7903-7904	-LRB-	denotes	(
T5153	7904-7910	NN	denotes	Tropix
T5154	7910-7911	-RRB-	denotes	)
T5155	7912-7914	IN	denotes	in
T5156	7915-7916	DT	denotes	a
T5157	7917-7931	NN	denotes	bioluminometer
T5158	7932-7933	-LRB-	denotes	(
T5159	7933-7939	NNP	denotes	Turner
T5160	7940-7949	NNP	denotes	Biosystem
T5161	7949-7950	-COMMA-	denotes	,
T5162	7951-7960	NNP	denotes	Sunnyvale
T5163	7960-7961	-COMMA-	denotes	,
T5164	7962-7964	NNP	denotes	CA
T5165	7964-7965	-COMMA-	denotes	,
T5166	7966-7969	NNP	denotes	USA
T5167	7969-7970	-RRB-	denotes	)
T5168	7972-7975	DT	denotes	The
T5169	7976-7981	NN	denotes	ratio
T5170	7982-7984	IN	denotes	of
T5171	7985-7992	NN	denotes	firefly
T5172	7993-8003	NN	denotes	luciferase
T5173	8004-8012	NN	denotes	activity
T5174	8013-8015	TO	denotes	to
T5175	8016-8031	NN	denotes	β-galactosidase
T5176	8032-8040	NN	denotes	activity
T5177	8041-8044	VB	denotes	was
T5178	8045-8054	VB	denotes	expressed
T5179	8055-8057	IN	denotes	as
T5180	8058-8066	JJ	denotes	relative
T5181	8067-8072	JJ	denotes	light
T5182	8073-8078	NN	denotes	units
T5682	8081-8084	NN	denotes	RNA
T5683	8085-8097	NN	denotes	interference
T5684	8098-8104	NN	denotes	Jurkat
T5685	8105-8107	CC	denotes	or
T5686	8108-8112	NN	denotes	U937
T5687	8113-8118	NN	denotes	cells
T5688	8119-8123	VB	denotes	were
T5689	8124-8135	VB	denotes	transfected
T5690	8136-8138	IN	denotes	by
T5691	8139-8154	NN	denotes	electroporation
T5692	8155-8160	VB	denotes	using
T5693	8161-8162	DT	denotes	a
T5694	8163-8170	JJ	denotes	Bio-Rad
T5695	8171-8180	NN	denotes	apparatus
T5696	8181-8182	-LRB-	denotes	(
T5697	8182-8189	NNP	denotes	Bio-Rad
T5698	8190-8202	NNP	denotes	Laboratories
T5699	8202-8203	-COMMA-	denotes	,
T5700	8204-8212	NNP	denotes	Hercules
T5701	8212-8213	-COMMA-	denotes	,
T5702	8214-8216	NNP	denotes	CA
T5703	8216-8217	-COMMA-	denotes	,
T5704	8218-8221	NNP	denotes	USA
T5705	8221-8222	-RRB-	denotes	)
T5706	8224-8231	RB	denotes	Briefly
T5707	8231-8232	-COMMA-	denotes	,
T5708	8233-8241	NN	denotes	aliquots
T5709	8242-8243	-LRB-	denotes	(
T5710	8243-8250	CD	denotes	5 × 106
T5711	8251-8256	NN	denotes	cells
T5712	8256-8257	-RRB-	denotes	)
T5713	8258-8262	VB	denotes	were
T5714	8263-8272	VB	denotes	suspended
T5715	8273-8275	IN	denotes	in
T5716	8276-8282	NN	denotes	0.3 ml
T5717	8283-8285	IN	denotes	of
T5718	8286-8290	NN	denotes	RPMI
T5719	8291-8295	CD	denotes	1640
T5720	8296-8308	VB	denotes	supplemented
T5721	8309-8313	IN	denotes	with
T5722	8314-8316	CD	denotes	20
T5723	8316-8317	NN	denotes	%
T5724	8318-8323	JJ	denotes	fetal
T5725	8324-8328	NN	denotes	calf
T5726	8329-8334	NN	denotes	serum
T5727	8335-8338	CC	denotes	and
T5728	8339-8348	VB	denotes	subjected
T5729	8349-8351	TO	denotes	to
T5730	8352-8353	DT	denotes	a
T5731	8354-8360	JJ	denotes	double
T5732	8361-8371	JJ	denotes	electrical
T5733	8372-8377	NN	denotes	pulse
T5734	8378-8379	-LRB-	denotes	(
T5735	8379-8385	NN	denotes	0.22 V
T5736	8385-8386	-COMMA-	denotes	,
T5737	8387-8393	NN	denotes	960 µF
T5738	8393-8394	-RRB-	denotes	)
T5739	8395-8397	IN	denotes	in
T5740	8398-8401	DT	denotes	the
T5741	8402-8410	NN	denotes	presence
T5742	8411-8413	IN	denotes	of
T5743	8414-8422	VB	denotes	annealed
T5744	8423-8428	NN	denotes	siRNA
T5745	8429-8430	-LRB-	denotes	(
T5746	8430-8438	NN	denotes	200 pmol
T5747	8438-8439	-RRB-	denotes	)
T5748	8439-8440	-COLON-	denotes	;
T5749	8441-8455	VB	denotes	electroporated
T5750	8456-8461	NN	denotes	cells
T5751	8462-8466	VB	denotes	were
T5752	8467-8473	VB	denotes	washed
T5753	8474-8477	CC	denotes	and
T5754	8478-8486	VB	denotes	cultured
T5755	8487-8489	IN	denotes	in
T5756	8490-8498	JJ	denotes	complete
T5757	8499-8505	NN	denotes	medium
T5758	8507-8510	NN	denotes	RNA
T5759	8511-8523	NN	denotes	interference
T5760	8524-8527	VB	denotes	was
T5761	8528-8537	VB	denotes	performed
T5762	8538-8542	IN	denotes	with
T5763	8542-8543	-COLON-	denotes	:
T5764	8544-8549	NN	denotes	siRNA
T5765	8550-8553	NN	denotes	Tat
T5766	8554-8559	NN	denotes	sense
T5767	8559-8560	-COMMA-	denotes	,
T5768	8561-8582	NN	denotes	CUGCUUGUACCAAUUGCUAUU
T5769	8583-8586	CC	denotes	and
T5770	8587-8592	NN	denotes	siRNA
T5771	8593-8596	NN	denotes	Tat
T5772	8597-8606	JJ	denotes	antisense
T5773	8606-8607	-COMMA-	denotes	,
T5774	8608-8629	NNP	denotes	UAGCAAUUGGUACAAGCAGUU
T5775	8629-8630	-COLON-	denotes	;
T5776	8631-8636	NN	denotes	siRNA
T5777	8637-8644	NN	denotes	control
T5778	8645-8650	NN	denotes	sense
T5779	8650-8651	-COMMA-	denotes	,
T5780	8652-8664	NN	denotes	CUGCUUGUCACA
T5781	8665-8674	NN	denotes	AUUGCUAUU
T5782	8675-8678	CC	denotes	and
T5783	8679-8684	NN	denotes	siRNA
T5784	8685-8692	NN	denotes	control
T5785	8693-8702	JJ	denotes	antisense
T5786	8702-8703	-COMMA-	denotes	,
T5787	8704-8725	NNP	denotes	UAGCAAUUGUGACAAGCAGUU
T5788	8727-8730	NN	denotes	RNA
T5789	8731-8743	NN	denotes	interference
T5790	8744-8746	IN	denotes	of
T5791	8747-8750	NN	denotes	p65
T5792	8751-8754	CC	denotes	and
T5793	8755-8760	NN	denotes	IκB-α
T5794	8761-8764	VB	denotes	was
T5795	8765-8774	VB	denotes	performed
T5796	8775-8779	IN	denotes	with
T5797	8780-8785	NN	denotes	SMART
T5798	8786-8790	NN	denotes	pool
T5799	8791-8796	NN	denotes	siRNA
T5800	8797-8800	NN	denotes	p65
T5801	8801-8804	CC	denotes	and
T5802	8805-8810	NN	denotes	IκB-α
T5803	8811-8812	-LRB-	denotes	(
T5804	8812-8821	NNP	denotes	Dharmacon
T5805	8821-8822	-COMMA-	denotes	,
T5806	8823-8830	NNP	denotes	Chicago
T5807	8830-8831	-COMMA-	denotes	,
T5808	8832-8834	NNP	denotes	IL
T5809	8834-8835	-COMMA-	denotes	,
T5810	8836-8839	NNP	denotes	USA
T5811	8839-8840	-RRB-	denotes	)
T6049	8843-8854	VB	denotes	Pseudotyped
T6050	8855-8862	NN	denotes	virions
T6051	8863-8866	CC	denotes	and
T6052	8867-8873	JJ	denotes	single
T6053	8874-8879	NN	denotes	round
T6054	8880-8889	NN	denotes	infection
T6055	8890-8894	NN	denotes	293T
T6056	8895-8900	NN	denotes	cells
T6057	8901-8902	-LRB-	denotes	(
T6058	8902-8909	CD	denotes	1 × 107
T6059	8909-8910	-RRB-	denotes	)
T6060	8911-8915	VB	denotes	were
T6061	8916-8927	VB	denotes	transfected
T6062	8928-8932	IN	denotes	with
T6063	8933-8948	NN	denotes	pNL4-3.Luc.R-E-
T6064	8949-8951	CC	denotes	or
T6065	8952-8972	NN	denotes	pNL4-3.FLAG-Tat.R-E-
T6066	8973-8974	-LRB-	denotes	(
T6067	8974-8979	NN	denotes	10 µg
T6068	8979-8980	-RRB-	denotes	)
T6069	8981-8989	RB	denotes	together
T6070	8990-8994	IN	denotes	with
T6071	8995-9000	NN	denotes	pHXB2
T6072	9001-9004	NN	denotes	Env
T6073	9005-9006	-LRB-	denotes	(
T6074	9006-9011	NN	denotes	10 µg
T6075	9011-9012	-RRB-	denotes	)
T6076	9012-9013	-COMMA-	denotes	,
T6078	9018-9022	JJ	denotes	48-h
T6079	9023-9040	JJ	denotes	post-transfection
T6080	9041-9045	NN	denotes	cell
T6081	9046-9057	NN	denotes	supernatant
T6082	9058-9061	VB	denotes	was
T6083	9062-9071	VB	denotes	collected
T6084	9073-9086	JJ	denotes	Enzyme-linked
T6085	9087-9100	JJ	denotes	immunosorbent
T6086	9101-9106	NN	denotes	assay
T6087	9107-9108	-LRB-	denotes	(
T6088	9108-9113	NN	denotes	ELISA
T6089	9113-9114	-RRB-	denotes	)
T6090	9115-9120	VB	denotes	using
T6091	9121-9129	JJ	denotes	anti-p24
T6092	9130-9138	NN	denotes	antibody
T6093	9139-9147	VB	denotes	measured
T6094	9148-9154	NN	denotes	virion
T6095	9155-9168	NN	denotes	concentration
T6096	9170-9175	NN	denotes	PBMCs
T6097	9175-9176	-COMMA-	denotes	,
T6098	9177-9183	NN	denotes	Jurkat
T6099	9184-9186	CC	denotes	or
T6100	9187-9191	NN	denotes	U937
T6101	9192-9197	NN	denotes	cells
T6102	9198-9199	-LRB-	denotes	(
T6103	9199-9206	CD	denotes	5 × 107
T6104	9206-9207	-RRB-	denotes	)
T6105	9208-9212	VB	denotes	were
T6106	9213-9221	VB	denotes	infected
T6107	9222-9226	IN	denotes	with
T6108	9227-9231	NN	denotes	HXB2
T6109	9232-9247	JJ	denotes	Env-pseudotyped
T6110	9248-9255	NN	denotes	virions
T6111	9256-9257	-LRB-	denotes	(
T6112	9257-9263	CD	denotes	500 ng
T6113	9264-9266	IN	denotes	of
T6114	9267-9270	NN	denotes	p24
T6115	9270-9271	-RRB-	denotes	)
T6116	9272-9274	IN	denotes	by
T6117	9275-9288	NN	denotes	spinoculation
T6118	9288-9289	-COMMA-	denotes	,
T6119	9290-9292	IN	denotes	as
T6120	9293-9303	RB	denotes	previously
T6121	9304-9313	VB	denotes	described
T6122	9314-9315	-LRB-	denotes	(
T6123	9315-9317	CD	denotes	50
T6124	9317-9318	-RRB-	denotes	)
T6718	10933-10935	FW	denotes	In
T6719	10936-10941	FW	denotes	vitro
T6720	10942-10953	NN	denotes	translation
T6721	10954-10962	NN	denotes	HA-IκB-α
T6722	10962-10963	-COMMA-	denotes	,
T6723	10964-10967	NN	denotes	p65
T6724	10968-10971	CC	denotes	and
T6725	10972-10975	NN	denotes	Tat
T6726	10976-10980	VB	denotes	were
T6727	10981-10990	VB	denotes	expressed
T6728	10991-10996	IN	denotes	under
T6729	10997-11000	DT	denotes	the
T6730	11001-11003	NN	denotes	T7
T6731	11004-11012	NN	denotes	promoter
T6732	11013-11016	CC	denotes	and
T6733	11017-11019	FW	denotes	in
T6734	11020-11025	FW	denotes	vitro
T6735	11026-11036	VB	denotes	translated
T6736	11037-11042	VB	denotes	using
T6737	11043-11046	DT	denotes	the
T6738	11047-11050	NN	denotes	TnT
T6739	11051-11056	JJ	denotes	quick
T6740	11057-11064	VB	denotes	coupled
T6741	11065-11090	NN	denotes	transcription/translation
T6742	11091-11097	NN	denotes	system
T6743	11098-11099	-LRB-	denotes	(
T6744	11099-11106	NN	denotes	Promega
T6745	11106-11107	-RRB-	denotes	)
T6746	11107-11108	-COMMA-	denotes	,
T6747	11109-11111	IN	denotes	as
T6748	11112-11122	RB	denotes	previously
T6749	11123-11131	VB	denotes	reported
T6750	11132-11133	-LRB-	denotes	(
T6751	11133-11135	CD	denotes	50
T6752	11135-11136	-RRB-	denotes	)
T6753	11138-11145	NN	denotes	Details
T6754	11146-11149	VB	denotes	are
T6755	11150-11159	VB	denotes	described
T6756	11160-11162	IN	denotes	in
T6757	11163-11176	NNP	denotes	Supplementary
T6758	11177-11181	NNP	denotes	Data
T6882	11184-11203	NN	denotes	Immunoprecipitation
T6883	11204-11209	NN	denotes	assay
T6884	11210-11213	CC	denotes	and
T6885	11214-11222	NN	denotes	GST-pull
T6886	11223-11227	RP	denotes	down
T6887	11228-11247	NN	denotes	Immunoprecipitation
T6888	11247-11248	-COMMA-	denotes	,
T6889	11249-11257	NNP	denotes	GST-pull
T6890	11258-11262	IN	denotes	down
T6891	11262-11263	-COMMA-	denotes	,
T6892	11264-11267	CC	denotes	and
T6893	11268-11278	NN	denotes	production
T6894	11279-11281	IN	denotes	of
T7016	11417-11426	JJ	denotes	Real-time
T7017	11427-11430	NN	denotes	PCR
T7018	11431-11436	JJ	denotes	Total
T7019	11437-11440	NN	denotes	RNA
T7020	11441-11444	VB	denotes	was
T7021	11445-11454	VB	denotes	extracted
T7022	11455-11459	IN	denotes	from
T7023	11460-11465	NN	denotes	cells
T7024	11466-11468	IN	denotes	by
T7025	11469-11474	VB	denotes	using
T7026	11475-11478	DT	denotes	the
T7027	11479-11485	NN	denotes	TRIzol
T7028	11486-11493	NN	denotes	reagent
T7029	11494-11495	-LRB-	denotes	(
T7030	11495-11505	NN	denotes	Invitrogen
T7031	11505-11506	-RRB-	denotes	)
T7032	11506-11507	-COLON-	denotes	;
T7033	11508-11511	NN	denotes	RNA
T7034	11512-11520	NN	denotes	aliquots
T7035	11521-11522	-LRB-	denotes	(
T7036	11522-11528	NN	denotes	200 ng
T7037	11528-11529	-RRB-	denotes	)
T7038	11530-11534	VB	denotes	were
T7039	11535-11542	JJ	denotes	reverse
T7040	11543-11554	VB	denotes	transcribed
T7041	11555-11560	VB	denotes	using
T7042	11561-11567	NNP	denotes	Random
T7043	11568-11575	NNP	denotes	Examers
T7044	11576-11577	-LRB-	denotes	(
T7045	11577-11582	NNP	denotes	Roche
T7046	11582-11583	-RRB-	denotes	)
T7047	11584-11587	CC	denotes	and
T7048	11588-11599	NN	denotes	Superscript
T7049	11600-11603	CD	denotes	III
T7050	11604-11611	JJ	denotes	Reverse
T7051	11612-11625	NN	denotes	Transcriptase
T7052	11626-11627	-LRB-	denotes	(
T7053	11627-11637	NN	denotes	Invitrogen
T7054	11637-11638	-RRB-	denotes	)
T7055	11638-11639	-COMMA-	denotes	,
T7056	11640-11649	VB	denotes	according
T7057	11650-11652	TO	denotes	to
T7058	11653-11656	DT	denotes	the
T7059	11657-11669	NN	denotes	manufacturer
T7060	11669-11671	POS	denotes	's
T7061	11672-11680	NN	denotes	protocol
T7062	11682-11691	JJ	denotes	Real-time
T7063	11692-11695	NN	denotes	PCR
T7064	11696-11699	VB	denotes	was
T7065	11700-11709	VB	denotes	performed
T7066	11710-11714	IN	denotes	with
T7067	11715-11718	DT	denotes	the
T7068	11719-11721	NN	denotes	iQ
T7069	11722-11727	NNP	denotes	Green
T7070	11728-11733	NNP	denotes	Super
T7071	11734-11737	NN	denotes	mix
T7072	11738-11739	-LRB-	denotes	(
T7073	11739-11746	NNP	denotes	Bio-Rad
T7074	11747-11759	NNP	denotes	Laboratories
T7075	11759-11760	-RRB-	denotes	)
T7076	11761-11764	CC	denotes	and
T7077	11765-11772	VB	denotes	carried
T7078	11773-11776	RP	denotes	out
T7079	11777-11781	IN	denotes	with
T7080	11782-11785	DT	denotes	the
T7081	11786-11793	NN	denotes	iCycler
T7082	11794-11796	NN	denotes	iQ
T7083	11797-11806	JJ	denotes	Real-Time
T7084	11807-11816	NN	denotes	detection
T7085	11817-11823	NN	denotes	system
T7086	11824-11825	-LRB-	denotes	(
T7087	11825-11832	NNP	denotes	Bio-Rad
T7088	11833-11845	NNP	denotes	Laboratories
T7089	11845-11846	-RRB-	denotes	)
T7090	11847-11852	IN	denotes	under
T7091	11853-11856	DT	denotes	the
T7092	11857-11866	JJ	denotes	following
T7093	11867-11877	NN	denotes	conditions
T7094	11877-11878	-COLON-	denotes	:
T7095	11879-11883	NN	denotes	95°C
T7096	11883-11884	-COMMA-	denotes	,
T7097	11885-11890	NN	denotes	1 min
T7098	11890-11891	-COLON-	denotes	;
T7099	11892-11893	-LRB-	denotes	(
T7100	11893-11897	NN	denotes	94°C
T7101	11897-11898	-COMMA-	denotes	,
T7102	11899-11903	NN	denotes	10 s
T7103	11903-11904	-COLON-	denotes	;
T7104	11905-11909	NN	denotes	60°C
T7105	11909-11910	-COMMA-	denotes	,
T7106	11911-11915	NN	denotes	30 s
T7107	11915-11916	-RRB-	denotes	)
T7108	11917-11920	CD	denotes	×40
T7109	11922-11929	NN	denotes	Primers
T7110	11930-11933	IN	denotes	for
T7111	11934-11937	NN	denotes	Tat
T7112	11938-11941	CC	denotes	and
T7113	11942-11948	NN	denotes	MIP-1α
T7114	11949-11952	VB	denotes	are
T7115	11953-11959	VB	denotes	listed
T7116	11960-11962	IN	denotes	in
T7117	11963-11976	NNP	denotes	Supplementary
T7118	11977-11982	NNP	denotes	Data.
T7119	11983-11992	NNP	denotes	Real-time
T7120	11993-11996	NN	denotes	PCR
T7121	11997-11999	IN	denotes	of
T7122	12000-12004	NN	denotes	CSF3
T7123	12004-12005	-COMMA-	denotes	,
T7124	12006-12009	NN	denotes	LTA
T7125	12009-12010	-COMMA-	denotes	,
T7126	12011-12017	NN	denotes	NFKBIA
T7127	12017-12018	-COMMA-	denotes	,
T7128	12019-12023	NN	denotes	TLR2
T7129	12023-12024	-COMMA-	denotes	,
T7130	12025-12030	NN	denotes	GAPDH
T7131	12031-12034	CC	denotes	and
T7132	12035-12039	NN	denotes	ACTB
T7133	12040-12043	VB	denotes	was
T7134	12044-12053	VB	denotes	performed
T7135	12054-12059	VB	denotes	using
T7136	12060-12063	DT	denotes	the
T7137	12064-12067	NN	denotes	RT2
T7138	12068-12076	NN	denotes	profiler
T7139	12077-12080	NN	denotes	PCR
T7140	12081-12092	JJ	denotes	Array-Human
T7141	12093-12098	NN	denotes	NF-κB
T7142	12099-12108	NN	denotes	signaling
T7143	12109-12116	NN	denotes	pathway
T7144	12117-12118	-LRB-	denotes	(
T7145	12118-12124	NNP	denotes	QIAGEN
T7146	12125-12133	NNP	denotes	Sciences
T7147	12133-12134	-COMMA-	denotes	,
T7148	12135-12137	NNP	denotes	MD
T7149	12137-12138	-COMMA-	denotes	,
T7150	12139-12142	NNP	denotes	USA
T7151	12142-12143	-RRB-	denotes	)
T7152	12145-12154	NN	denotes	Reactions
T7153	12155-12159	VB	denotes	were
T7154	12160-12167	VB	denotes	carried
T7155	12168-12171	RP	denotes	out
T7156	12172-12174	IN	denotes	in
T7157	12175-12185	NN	denotes	triplicate
T7158	12185-12186	-COMMA-	denotes	,
T7159	12187-12190	CC	denotes	and
T7160	12191-12195	NN	denotes	gene
T7161	12196-12206	NN	denotes	expression
T7162	12207-12213	NN	denotes	levels
T7163	12214-12218	VB	denotes	were
T7164	12219-12229	VB	denotes	calculated
T7165	12230-12238	JJ	denotes	relative
T7166	12239-12241	TO	denotes	to
T7167	12242-12247	NN	denotes	GAPDH
T7168	12248-12252	NN	denotes	mRNA
T7169	12253-12259	NN	denotes	levels
T7170	12260-12262	IN	denotes	as
T7171	12263-12273	JJ	denotes	endogenous
T7172	12274-12281	NN	denotes	control
T7173	12283-12291	JJ	denotes	Relative
T7174	12292-12302	NN	denotes	expression
T7175	12303-12306	VB	denotes	was
T7176	12307-12317	VB	denotes	calculated
T7177	12318-12320	IN	denotes	as
T7178	12321-12322	CD	denotes	2
T7179	12322-12323	-LRB-	denotes	(
T7180	12323-12325	NN	denotes	Ct
T7181	12326-12330	NN	denotes	gene
T7182	12331-12336	IN	denotes	under
T7183	12337-12355	NN	denotes	investigation − Ct
T7184	12356-12361	NN	denotes	GAPDH
T7185	12361-12362	-RRB-	denotes	)
T8159	14748-14759	JJ	denotes	Statistical
T8160	14760-14768	NN	denotes	analysis
T8161	14769-14780	JJ	denotes	Statistical
T8162	14781-14789	NN	denotes	analysis
T8163	14790-14793	VB	denotes	was
T8164	14794-14803	VB	denotes	performed
T8165	14804-14806	IN	denotes	by
T8166	14807-14815	JJ	denotes	two-tail
T8167	14816-14824	JJ	denotes	unpaired
T8168	14825-14832	NN	denotes	Student
T8169	14832-14834	POS	denotes	's
T8170	14835-14841	NN	denotes	t-test
T8171	14843-14847	NN	denotes	Data
T8172	14848-14852	VB	denotes	were
T8173	14853-14861	VB	denotes	reported
T8174	14862-14864	IN	denotes	as
T8175	14865-14875	NN	denotes	means ± SE
T8176	14877-14888	NN	denotes	Differences
T8177	14889-14896	IN	denotes	between
T8178	14897-14900	DT	denotes	the
T8179	14901-14906	NN	denotes	means
T8180	14907-14911	VB	denotes	were
T8181	14912-14922	VB	denotes	considered
T8182	14923-14925	IN	denotes	as
T8183	14926-14939	RB	denotes	statistically
T8184	14940-14951	JJ	denotes	significant
T8185	14952-14954	IN	denotes	at
T8186	14955-14958	DT	denotes	the
T8187	14959-14961	CD	denotes	95
T8188	14961-14962	NN	denotes	%
T8189	14963-14968	NN	denotes	level
T8190	14969-14970	-LRB-	denotes	(
T8191	14970-14972	NN	denotes	P
T8192	14972-14973	SYM	denotes	<
T8193	14973-14978	CD	denotes	0.05
T8194	14978-14979	-RRB-	denotes	)
T8554	14991-14994	NN	denotes	Tat
T8555	14995-15003	VB	denotes	enhances
T8556	15004-15007	DT	denotes	the
T8557	15008-15013	NN	denotes	NF-κB
T8558	15014-15022	NN	denotes	activity
T8559	15023-15025	IN	denotes	by
T8560	15026-15035	VB	denotes	hijacking
T8561	15036-15041	NN	denotes	IκB-α
T8562	15042-15045	CC	denotes	and
T8563	15046-15056	VB	denotes	inhibiting
T8564	15057-15060	DT	denotes	the
T8565	15061-15076	JJ	denotes	post-activation
T8566	15077-15081	NN	denotes	turn
T8567	15082-15085	RP	denotes	off
T8568	15086-15088	IN	denotes	of
T8569	15089-15094	NN	denotes	NF-κB
T8570	15095-15097	PRP	denotes	We
T8571	15098-15106	VB	denotes	analysed
T8572	15107-15110	DT	denotes	the
T8573	15111-15118	NN	denotes	kinetic
T8574	15119-15121	IN	denotes	of
T8575	15122-15127	NN	denotes	NF-κB
T8576	15128-15138	NN	denotes	activation
T8577	15139-15141	IN	denotes	in
T8578	15142-15148	JJ	denotes	single
T8579	15149-15154	JJ	denotes	round
T8580	15155-15160	NN	denotes	HIV-1
T8581	15161-15170	NN	denotes	infection
T8582	15171-15173	IN	denotes	by
T8583	15174-15184	VB	denotes	modulating
T8584	15185-15188	DT	denotes	the
T8585	15189-15199	NN	denotes	expression
T8586	15200-15202	IN	denotes	of
T8587	15203-15206	NN	denotes	Tat
T8588	15207-15211	IN	denotes	with
T8589	15212-15215	NN	denotes	RNA
T8590	15216-15228	NN	denotes	interference
T8591	15230-15236	NN	denotes	Jurkat
T8592	15237-15242	NN	denotes	cells
T8593	15243-15247	VB	denotes	were
T8594	15248-15259	VB	denotes	transfected
T8595	15260-15264	IN	denotes	with
T8596	15265-15270	NN	denotes	siRNA
T8597	15271-15274	NN	denotes	Tat
T8598	15274-15275	-COMMA-	denotes	,
T8599	15276-15281	NN	denotes	siRNA
T8600	15282-15289	NN	denotes	control
T8601	15289-15290	-COMMA-	denotes	,
T8602	15291-15293	CC	denotes	or
T8603	15294-15298	VB	denotes	left
T8604	15299-15312	JJ	denotes	untransfected
T8605	15312-15313	-COMMA-	denotes	,
T8606	15314-15317	CC	denotes	and
T8607	15318-15322	NN	denotes	24 h
T8608	15323-15328	JJ	denotes	later
T8609	15329-15334	NN	denotes	cells
T8610	15335-15339	VB	denotes	were
T8611	15340-15348	VB	denotes	infected
T8612	15349-15353	IN	denotes	with
T8613	15354-15358	NN	denotes	HXB2
T8614	15359-15374	JJ	denotes	Env-pseudotyped
T8615	15375-15389	NN	denotes	NL4-3.Luc.R−E−
T8616	15390-15397	NN	denotes	virions
T8617	15399-15401	IN	denotes	By
T8618	15402-15418	JJ	denotes	cytofluorimetric
T8619	15419-15427	NN	denotes	analysis
T8620	15427-15428	-COMMA-	denotes	,
T8621	15429-15432	CD	denotes	∼40
T8622	15432-15433	NN	denotes	%
T8623	15434-15436	IN	denotes	of
T8624	15437-15442	NN	denotes	cells
T8625	15443-15446	VB	denotes	was
T8626	15447-15464	JJ	denotes	siRNA-transfected
T8627	15465-15468	CC	denotes	and
T8628	15469-15483	JJ	denotes	HIV-1-infected
T8629	15484-15486	IN	denotes	at
T8630	15487-15491	JJ	denotes	12-h
T8631	15492-15506	JJ	denotes	post-infection
T8632	15507-15508	-LRB-	denotes	(
T8633	15508-15521	JJ	denotes	Supplementary
T8634	15522-15528	NN	denotes	Figure
T8635	15529-15531	NN	denotes	S1
T8636	15531-15532	-RRB-	denotes	)
T8637	15534-15537	DT	denotes	The
T8638	15538-15548	NN	denotes	expression
T8639	15549-15551	IN	denotes	of
T8640	15552-15555	NN	denotes	Tat
T8641	15556-15559	VB	denotes	was
T8642	15560-15568	VB	denotes	detected
T8643	15569-15571	IN	denotes	at
T8644	15572-15575	JJ	denotes	1-h
T8645	15576-15590	JJ	denotes	post-infection
T8646	15591-15594	CC	denotes	and
T8647	15595-15608	RB	denotes	progressively
T8648	15609-15618	VB	denotes	increased
T8649	15619-15621	RB	denotes	up
T8650	15622-15624	TO	denotes	to
T8651	15625-15629	VB	denotes	12 h
T8652	15630-15632	IN	denotes	in
T8653	15633-15646	JJ	denotes	untransfected
T8654	15647-15650	CC	denotes	and
T8655	15651-15656	NN	denotes	siRNA
T8656	15657-15676	JJ	denotes	control-transfected
T8657	15677-15682	NN	denotes	cells
T8658	15682-15683	-COMMA-	denotes	,
T8659	15684-15689	IN	denotes	while
T8660	15690-15692	PRP	denotes	it
T8661	15693-15696	VB	denotes	was
T8662	15697-15703	RB	denotes	barely
T8663	15704-15712	VB	denotes	detected
T8664	15713-15715	IN	denotes	in
T8665	15716-15721	NN	denotes	siRNA
T8666	15722-15737	JJ	denotes	Tat-transfected
T8667	15738-15743	NN	denotes	cells
T8668	15744-15745	-LRB-	denotes	(
T8669	15745-15751	NN	denotes	Figure
T8670	15752-15754	NN	denotes	1A
T8671	15754-15755	-RRB-	denotes	)
T8672	15757-15760	DT	denotes	The
T8673	15761-15774	JJ	denotes	LTR-dependent
T8674	15775-15785	NN	denotes	luciferase
T8675	15786-15796	NN	denotes	expression
T8676	15797-15800	VB	denotes	was
T8677	15801-15805	RB	denotes	also
T8678	15806-15819	RB	denotes	progressively
T8679	15820-15827	VB	denotes	induced
T8680	15828-15830	IN	denotes	at
T8681	15831-15833	CD	denotes	1-
T8682	15834-15836	TO	denotes	to
T8683	15837-15841	JJ	denotes	12-h
T8684	15842-15856	NN	denotes	post-infection
T8685	15857-15859	IN	denotes	in
T8686	15860-15873	JJ	denotes	untransfected
T8687	15874-15877	CC	denotes	and
T8688	15878-15883	NN	denotes	siRNA
T8689	15884-15903	JJ	denotes	control-transfected
T8690	15904-15909	NN	denotes	cells
T8691	15909-15910	-COMMA-	denotes	,
T8692	15911-15916	IN	denotes	while
T8693	15917-15919	PRP	denotes	it
T8694	15920-15923	VB	denotes	was
T8695	15924-15930	RB	denotes	barely
T8696	15931-15939	VB	denotes	observed
T8697	15940-15942	IN	denotes	in
T8698	15943-15948	NN	denotes	siRNA
T8699	15949-15964	JJ	denotes	Tat-transfected
T8700	15965-15970	NN	denotes	cells
T8701	15970-15971	-COMMA-	denotes	,
T8702	15972-15974	IN	denotes	as
T8703	15975-15983	VB	denotes	expected
T8704	15984-15987	IN	denotes	for
T8705	15988-15991	DT	denotes	the
T8706	15992-16005	JJ	denotes	Tat-dependent
T8707	16006-16021	NN	denotes	transactivation
T8708	16022-16024	IN	denotes	of
T8709	16025-16028	DT	denotes	the
T8710	16029-16034	NN	denotes	HIV-1
T8711	16035-16038	NN	denotes	LTR
T8712	16039-16040	-LRB-	denotes	(
T8713	16040-16046	NN	denotes	Figure
T8714	16047-16049	NN	denotes	1A
T8715	16049-16050	-RRB-	denotes	)
T8716	17245-17248	DT	denotes	The
T8717	17249-17260	JJ	denotes	progressive
T8718	17261-17269	NN	denotes	increase
T8719	17270-17272	IN	denotes	in
T8720	17273-17278	NN	denotes	NF-κB
T8721	17279-17287	NN	denotes	activity
T8722	17287-17288	-COMMA-	denotes	,
T8723	17289-17291	IN	denotes	as
T8724	17292-17300	VB	denotes	measured
T8725	17301-17303	IN	denotes	by
T8726	17304-17311	NN	denotes	p65–p50
T8727	17312-17319	NN	denotes	binding
T8728	17320-17322	TO	denotes	to
T8729	17323-17325	DT	denotes	an
T8730	17326-17331	NN	denotes	NF-κB
T8731	17332-17341	NN	denotes	consensus
T8732	17342-17357	NN	denotes	oligonucleotide
T8733	17358-17359	-LRB-	denotes	(
T8734	17359-17365	NN	denotes	Figure
T8735	17366-17368	NN	denotes	1B
T8736	17368-17369	-RRB-	denotes	)
T8737	17370-17373	CC	denotes	and
T8738	17374-17381	JJ	denotes	nuclear
T8739	17382-17385	NN	denotes	p65
T8740	17386-17387	-LRB-	denotes	(
T8741	17387-17393	NN	denotes	Figure
T8742	17394-17396	NN	denotes	1C
T8743	17396-17397	-COMMA-	denotes	,
T8744	17398-17405	NN	denotes	nucleus
T8745	17405-17406	-RRB-	denotes	)
T8746	17406-17407	-COMMA-	denotes	,
T8747	17408-17411	VB	denotes	was
T8748	17412-17420	VB	denotes	observed
T8749	17421-17423	IN	denotes	at
T8750	17424-17426	CD	denotes	1-
T8751	17427-17429	TO	denotes	to
T8752	17430-17433	JJ	denotes	3-h
T8753	17434-17448	NN	denotes	post-infection
T8754	17449-17451	IN	denotes	in
T8755	17452-17456	CC	denotes	both
T8756	17457-17469	JJ	denotes	Tat-positive
T8757	17470-17471	-LRB-	denotes	(
T8758	17471-17473	DT	denotes	no
T8759	17474-17479	NN	denotes	siRNA
T8760	17480-17482	CC	denotes	or
T8761	17483-17488	NN	denotes	siRNA
T8762	17489-17496	NN	denotes	control
T8763	17496-17497	-RRB-	denotes	)
T8764	17498-17501	CC	denotes	and
T8765	17502-17514	JJ	denotes	Tat-negative
T8766	17515-17516	-LRB-	denotes	(
T8767	17516-17521	NN	denotes	siRNA
T8768	17522-17525	NN	denotes	Tat
T8769	17525-17526	-RRB-	denotes	)
T8770	17527-17532	NN	denotes	cells
T8771	17532-17533	-COLON-	denotes	;
T8772	17534-17541	RB	denotes	however
T8773	17541-17542	-COMMA-	denotes	,
T8774	17543-17545	IN	denotes	at
T8775	17546-17550	JJ	denotes	12-h
T8776	17551-17565	NN	denotes	post-infection
T8777	17565-17566	-COMMA-	denotes	,
T8778	17567-17570	DT	denotes	the
T8779	17571-17574	NN	denotes	p65
T8780	17575-17578	NN	denotes	DNA
T8781	17579-17586	NN	denotes	binding
T8782	17587-17590	CC	denotes	and
T8783	17591-17598	JJ	denotes	nuclear
T8784	17599-17602	NN	denotes	p65
T8785	17603-17612	VB	denotes	persisted
T8786	17613-17621	VB	denotes	elevated
T8787	17622-17624	IN	denotes	in
T8788	17625-17633	NN	denotes	presence
T8789	17634-17636	IN	denotes	of
T8790	17637-17640	NN	denotes	Tat
T8791	17641-17642	-LRB-	denotes	(
T8792	17642-17644	DT	denotes	no
T8793	17645-17650	NN	denotes	siRNA
T8794	17651-17654	CC	denotes	and
T8795	17655-17660	NN	denotes	siRNA
T8796	17661-17668	NN	denotes	control
T8797	17668-17669	-RRB-	denotes	)
T8798	17669-17670	-COMMA-	denotes	,
T8799	17671-17676	IN	denotes	while
T8800	17677-17681	PRP	denotes	they
T8801	17682-17689	VB	denotes	dropped
T8802	17690-17692	IN	denotes	in
T8803	17693-17700	NN	denotes	absence
T8804	17701-17703	IN	denotes	of
T8805	17704-17707	NN	denotes	Tat
T8806	17708-17709	-LRB-	denotes	(
T8807	17709-17714	NN	denotes	siRNA
T8808	17715-17718	NN	denotes	Tat
T8809	17718-17719	-RRB-	denotes	)
T8810	17720-17721	-LRB-	denotes	(
T8811	17721-17727	NN	denotes	Figure
T8812	17728-17730	NN	denotes	1B
T8813	17731-17734	CC	denotes	and
T8814	17735-17736	NN	denotes	C
T8815	17736-17737	-RRB-	denotes	)
T8816	17739-17744	NN	denotes	IκB-α
T8817	17745-17748	VB	denotes	was
T8818	17749-17757	VB	denotes	degraded
T8819	17758-17760	IN	denotes	in
T8820	17761-17764	DT	denotes	the
T8821	17765-17772	NN	denotes	cytosol
T8822	17773-17779	IN	denotes	within
T8823	17780-17783	JJ	denotes	3-h
T8824	17784-17798	JJ	denotes	post-infection
T8825	17799-17802	CC	denotes	and
T8826	17803-17805	FW	denotes	de
T8827	17806-17810	FW	denotes	novo
T8828	17811-17822	VB	denotes	synthesized
T8829	17823-17825	IN	denotes	at
T8830	17826-17829	NN	denotes	6 h
T8831	17830-17834	IN	denotes	with
T8832	17835-17839	JJ	denotes	full
T8833	17840-17853	NN	denotes	replenishment
T8834	17854-17856	IN	denotes	at
T8835	17857-17861	JJ	denotes	12-h
T8836	17862-17876	NN	denotes	post-infection
T8837	17877-17879	IN	denotes	in
T8838	17880-17884	CC	denotes	both
T8839	17885-17897	JJ	denotes	Tat-positive
T8840	17898-17901	CC	denotes	and
T8841	17902-17914	JJ	denotes	Tat-negative
T8842	17915-17920	NN	denotes	cells
T8843	17921-17922	-LRB-	denotes	(
T8844	17922-17928	NN	denotes	Figure
T8845	17929-17931	NN	denotes	1C
T8846	17931-17932	-COMMA-	denotes	,
T8847	17933-17940	NN	denotes	cytosol
T8848	17940-17941	-RRB-	denotes	)
T8849	17943-17955	RB	denotes	Consistently
T8850	17955-17956	-COMMA-	denotes	,
T8851	17957-17960	DT	denotes	the
T8852	17961-17964	NN	denotes	IKK
T8853	17965-17973	NN	denotes	activity
T8854	17974-17977	VB	denotes	was
T8855	17978-17985	VB	denotes	induced
T8856	17986-17988	IN	denotes	at
T8857	17989-17992	JJ	denotes	1-h
T8858	17993-18007	NN	denotes	post-infection
T8859	18007-18008	-COMMA-	denotes	,
T8860	18009-18012	CC	denotes	and
T8861	18013-18022	VB	denotes	decreased
T8862	18023-18025	IN	denotes	at
T8863	18026-18029	JJ	denotes	3-h
T8864	18030-18044	JJ	denotes	post-infection
T8865	18045-18058	RB	denotes	independently
T8866	18059-18061	IN	denotes	of
T8867	18062-18065	DT	denotes	the
T8868	18066-18074	NN	denotes	presence
T8869	18075-18077	IN	denotes	of
T8870	18078-18081	NN	denotes	Tat
T8871	18082-18083	-LRB-	denotes	(
T8872	18083-18089	NN	denotes	Figure
T8873	18090-18092	NN	denotes	1D
T8874	18092-18093	-RRB-	denotes	)
T8875	18095-18102	JJ	denotes	Similar
T8876	18103-18111	NN	denotes	kinetics
T8877	18112-18114	IN	denotes	of
T8878	18115-18120	NN	denotes	NF-κB
T8879	18121-18129	NN	denotes	activity
T8880	18129-18130	-COMMA-	denotes	,
T8881	18131-18134	NN	denotes	IKK
T8882	18135-18145	NN	denotes	activation
T8883	18146-18149	CC	denotes	and
T8884	18150-18155	NN	denotes	IκB-α
T8885	18156-18180	NN	denotes	degradation/re-synthesis
T8886	18181-18185	VB	denotes	were
T8887	18186-18194	VB	denotes	observed
T8888	18195-18197	IN	denotes	in
T8889	18198-18210	JJ	denotes	single-round
T8890	18211-18216	NN	denotes	HIV-1
T8891	18217-18226	NN	denotes	infection
T8892	18227-18229	IN	denotes	of
T8893	18230-18235	NN	denotes	PBMCs
T8894	18236-18241	VB	denotes	using
T8895	18242-18243	DT	denotes	a
T8896	18244-18252	NN	denotes	cocktail
T8897	18253-18255	IN	denotes	of
T8898	18256-18270	NN	denotes	Azidothymidine
T8899	18271-18272	-LRB-	denotes	(
T8900	18272-18275	NN	denotes	AZT
T8901	18275-18276	-RRB-	denotes	)
T8902	18277-18280	CC	denotes	and
T8903	18281-18291	NN	denotes	Lamivudine
T8904	18292-18293	-LRB-	denotes	(
T8905	18293-18296	NN	denotes	3TC
T8906	18296-18297	-RRB-	denotes	)
T8907	18298-18300	IN	denotes	as
T8908	18301-18308	JJ	denotes	reverse
T8909	18309-18322	NN	denotes	transcriptase
T8910	18323-18333	NN	denotes	inhibitors
T8911	18334-18335	-LRB-	denotes	(
T8912	18335-18348	JJ	denotes	Supplementary
T8913	18349-18355	NNP	denotes	Figure
T8914	18356-18358	NN	denotes	S2
T8915	18358-18359	-RRB-	denotes	)
T8916	18361-18366	DT	denotes	These
T8917	18367-18374	NN	denotes	results
T8918	18375-18384	VB	denotes	indicated
T8919	18385-18389	IN	denotes	that
T8920	18390-18392	IN	denotes	in
T8921	18393-18405	JJ	denotes	single-round
T8922	18406-18411	NN	denotes	HIV-1
T8923	18412-18421	NN	denotes	infection
T8924	18422-18425	DT	denotes	the
T8925	18426-18431	NN	denotes	NF-κB
T8926	18432-18442	NN	denotes	activation
T8927	18443-18452	RB	denotes	initially
T8928	18453-18463	VB	denotes	correlated
T8929	18464-18468	IN	denotes	with
T8930	18469-18472	DT	denotes	the
T8931	18473-18476	NN	denotes	IKK
T8932	18477-18487	NN	denotes	activation
T8933	18488-18491	CC	denotes	and
T8934	18492-18497	JJ	denotes	IκB-α
T8935	18498-18509	NN	denotes	degradation
T8936	18510-18523	RB	denotes	independently
T8937	18524-18526	IN	denotes	of
T8938	18527-18530	DT	denotes	the
T8939	18531-18539	NN	denotes	presence
T8940	18540-18542	IN	denotes	of
T8941	18543-18546	NN	denotes	Tat
T8942	18546-18547	-COMMA-	denotes	,
T8943	18548-18551	CC	denotes	and
T8944	18552-18554	PRP	denotes	it
T8945	18555-18558	VB	denotes	was
T8946	18559-18563	VB	denotes	kept
T8947	18564-18572	VB	denotes	elevated
T8948	18573-18575	IN	denotes	in
T8949	18576-18584	NN	denotes	presence
T8950	18585-18587	IN	denotes	of
T8951	18588-18591	NN	denotes	Tat
T8952	18592-18601	VB	denotes	following
T8953	18602-18605	DT	denotes	the
T8954	18606-18611	NN	denotes	decay
T8955	18612-18614	IN	denotes	of
T8956	18615-18618	DT	denotes	the
T8957	18619-18622	NN	denotes	IKK
T8958	18623-18631	NN	denotes	activity
T8959	18632-18635	CC	denotes	and
T8960	18636-18639	JJ	denotes	new
T8961	18640-18649	NN	denotes	synthesis
T8962	18650-18652	IN	denotes	of
T8963	18653-18658	NN	denotes	IκB-α
T8964	18660-18664	RB	denotes	Next
T8965	18664-18665	-COMMA-	denotes	,
T8966	18666-18668	PRP	denotes	we
T8967	18669-18675	VB	denotes	tested
T8968	18676-18679	DT	denotes	the
T8969	18680-18686	JJ	denotes	single
T8970	18687-18693	NN	denotes	action
T8971	18694-18696	IN	denotes	of
T8972	18697-18700	NN	denotes	Tat
T8973	18701-18703	IN	denotes	on
T8974	18704-18707	DT	denotes	the
T8975	18708-18715	NN	denotes	kinetic
T8976	18716-18718	IN	denotes	of
T8977	18719-18724	NN	denotes	NF-κB
T8978	18725-18733	NN	denotes	activity
T8979	18734-18741	VB	denotes	induced
T8980	18742-18744	IN	denotes	by
T8981	18745-18748	NN	denotes	PMA
T8982	18750-18752	TO	denotes	To
T8983	18753-18757	DT	denotes	this
T8984	18758-18761	NN	denotes	end
T8985	18761-18762	-COMMA-	denotes	,
T8986	18763-18767	NN	denotes	HeLa
T8987	18768-18773	NN	denotes	cells
T8988	18774-18778	VB	denotes	were
T8989	18779-18790	VB	denotes	transfected
T8990	18791-18795	IN	denotes	with
T8991	18796-18808	NN	denotes	p3x-FLAG-Tat
T8992	18808-18809	-COMMA-	denotes	,
T8993	18810-18812	CC	denotes	or
T8994	18813-18818	JJ	denotes	empty
T8995	18819-18825	NN	denotes	vector
T8996	18825-18826	-COMMA-	denotes	,
T8997	18827-18837	VB	denotes	stimulated
T8998	18838-18842	IN	denotes	with
T8999	18843-18846	NN	denotes	PMA
T9000	18847-18850	IN	denotes	for
T9001	18851-18856	NN	denotes	5 min
T9002	18856-18857	-COMMA-	denotes	,
T9003	18858-18861	CC	denotes	and
T9004	18862-18873	RB	denotes	extensively
T9005	18874-18880	VB	denotes	washed
T9006	18881-18883	TO	denotes	to
T9007	18884-18889	VB	denotes	avoid
T9008	18890-18893	DT	denotes	the
T9009	18894-18905	JJ	denotes	oscillatory
T9010	18906-18913	NN	denotes	kinetic
T9011	18914-18916	IN	denotes	of
T9012	18917-18922	NN	denotes	NF-κB
T9013	18923-18933	NN	denotes	activation
T9014	18934-18935	-LRB-	denotes	(
T9015	18935-18940	CD	denotes	17,18
T9016	18940-18941	-RRB-	denotes	)
T9017	18943-18945	IN	denotes	In
T9018	18946-18959	JJ	denotes	un-stimulated
T9019	18960-18965	NN	denotes	cells
T9020	18965-18966	-COMMA-	denotes	,
T9021	18967-18970	DT	denotes	the
T9022	18971-18974	NN	denotes	p65
T9023	18975-18978	NN	denotes	DNA
T9024	18979-18986	NN	denotes	binding
T9025	18987-18988	-LRB-	denotes	(
T9026	18988-18994	NN	denotes	Figure
T9027	18995-18997	NN	denotes	2A
T9028	18997-18998	-RRB-	denotes	)
T9029	18999-19002	CC	denotes	and
T9030	19003-19010	JJ	denotes	nuclear
T9031	19011-19014	NN	denotes	p65
T9032	19015-19016	-LRB-	denotes	(
T9033	19016-19022	NN	denotes	Figure
T9034	19023-19025	NN	denotes	2B
T9035	19025-19026	-COMMA-	denotes	,
T9036	19027-19034	NN	denotes	nucleus
T9037	19034-19035	-RRB-	denotes	)
T9038	19036-19040	VB	denotes	were
T9039	19041-19049	RB	denotes	slightly
T9040	19050-19058	VB	denotes	enhanced
T9041	19059-19061	IN	denotes	by
T9042	19062-19065	NN	denotes	Tat
T9043	19065-19066	-COMMA-	denotes	,
T9044	19067-19072	IN	denotes	while
T9045	19073-19076	DT	denotes	the
T9046	19077-19082	JJ	denotes	IκB-α
T9047	19083-19090	NN	denotes	content
T9048	19091-19094	VB	denotes	was
T9049	19095-19098	RB	denotes	not
T9050	19099-19112	RB	denotes	significantly
T9051	19113-19121	VB	denotes	affected
T9052	19122-19123	-LRB-	denotes	(
T9053	19123-19129	NN	denotes	Figure
T9054	19130-19132	NN	denotes	2B
T9055	19132-19133	-COMMA-	denotes	,
T9056	19134-19141	NN	denotes	cytosol
T9057	19141-19142	-RRB-	denotes	)
T9058	19144-19153	JJ	denotes	Transient
T9059	19154-19165	NN	denotes	stimulation
T9060	19166-19170	IN	denotes	with
T9061	19171-19174	NN	denotes	PMA
T9062	19175-19184	VB	denotes	increased
T9063	19185-19188	DT	denotes	the
T9064	19189-19194	NN	denotes	NF-κB
T9065	19195-19198	NN	denotes	DNA
T9066	19199-19206	NN	denotes	binding
T9067	19207-19215	NN	denotes	activity
T9068	19216-19219	CC	denotes	and
T9069	19220-19227	JJ	denotes	nuclear
T9070	19228-19231	NN	denotes	p65
T9071	19232-19234	IN	denotes	at
T9072	19235-19240	NN	denotes	5 min
T9073	19241-19248	VB	denotes	peaking
T9074	19249-19251	IN	denotes	at
T9075	19252-19262	NN	denotes	60–120 min
T9076	19263-19276	RB	denotes	independently
T9077	19277-19279	IN	denotes	of
T9078	19280-19283	DT	denotes	the
T9079	19284-19292	NN	denotes	presence
T9080	19293-19295	IN	denotes	of
T9081	19296-19299	NN	denotes	Tat
T9082	19300-19301	-LRB-	denotes	(
T9083	19301-19307	NN	denotes	Figure
T9084	19308-19310	NN	denotes	2A
T9085	19311-19314	CC	denotes	and
T9086	19315-19316	NN	denotes	B
T9087	19316-19317	-RRB-	denotes	)
T9088	19317-19318	-COLON-	denotes	;
T9089	19319-19326	RB	denotes	however
T9090	19326-19327	-COMMA-	denotes	,
T9091	19328-19333	IN	denotes	while
T9092	19334-19337	DT	denotes	the
T9093	19338-19341	NN	denotes	p65
T9094	19342-19345	NN	denotes	DNA
T9095	19346-19353	NN	denotes	binding
T9096	19354-19357	CC	denotes	and
T9097	19358-19365	JJ	denotes	nuclear
T9098	19366-19369	NN	denotes	p65
T9099	19370-19379	VB	denotes	persisted
T9100	19380-19388	VB	denotes	elevated
T9101	19389-19391	IN	denotes	at
T9102	19392-19399	JJ	denotes	240-min
T9103	19400-19414	JJ	denotes	post-treatment
T9104	19415-19417	IN	denotes	in
T9105	19418-19430	JJ	denotes	Tat-positive
T9106	19431-19436	NN	denotes	cells
T9107	19436-19437	-COMMA-	denotes	,
T9108	19438-19442	PRP	denotes	they
T9109	19443-19450	VB	denotes	dropped
T9110	19451-19453	IN	denotes	in
T9111	19454-19466	JJ	denotes	Tat-negative
T9112	19467-19472	NN	denotes	cells
T9113	19473-19474	-LRB-	denotes	(
T9114	19474-19480	NN	denotes	Figure
T9115	19481-19483	NN	denotes	2A
T9116	19484-19487	CC	denotes	and
T9117	19488-19489	NN	denotes	B
T9118	19489-19490	-RRB-	denotes	)
T9119	19492-19494	IN	denotes	In
T9120	19495-19503	NN	denotes	addition
T9121	19503-19504	-COMMA-	denotes	,
T9122	19505-19508	NN	denotes	Tat
T9123	19509-19514	VB	denotes	bound
T9124	19515-19517	TO	denotes	to
T9125	19518-19521	DT	denotes	the
T9126	19522-19527	NN	denotes	NF-κB
T9127	19528-19543	NN	denotes	oligonucleotide
T9128	19544-19546	IN	denotes	in
T9129	19547-19560	JJ	denotes	un-stimulated
T9130	19561-19566	NN	denotes	cells
T9131	19567-19570	CC	denotes	and
T9132	19571-19574	PRP-DOLLAR-	denotes	its
T9133	19575-19582	NN	denotes	binding
T9134	19583-19592	VB	denotes	increased
T9135	19593-19602	VB	denotes	following
T9136	19603-19606	NN	denotes	PMA
T9137	19607-19618	NN	denotes	stimulation
T9138	19619-19620	-LRB-	denotes	(
T9139	19620-19626	NN	denotes	Figure
T9140	19627-19629	NN	denotes	2A
T9141	19629-19630	-RRB-	denotes	)
T9142	19630-19631	-COMMA-	denotes	,
T9143	19632-19642	VB	denotes	suggesting
T9144	19643-19647	IN	denotes	that
T9145	19648-19651	DT	denotes	the
T9146	19652-19657	JJ	denotes	viral
T9147	19658-19665	NN	denotes	protein
T9148	19666-19669	VB	denotes	was
T9149	19670-19671	DT	denotes	a
T9150	19672-19681	NN	denotes	component
T9151	19682-19684	IN	denotes	of
T9152	19685-19688	DT	denotes	the
T9153	19689-19694	NN	denotes	NF-κB
T9154	19695-19702	NN	denotes	complex
T9155	19703-19708	VB	denotes	bound
T9156	19709-19711	TO	denotes	to
T9157	19712-19715	NN	denotes	DNA
T9158	19717-19728	NN	denotes	Degradation
T9159	19729-19731	IN	denotes	of
T9160	19732-19737	NN	denotes	IκB-α
T9161	19738-19741	VB	denotes	was
T9162	19742-19755	RB	denotes	progressively
T9163	19756-19763	VB	denotes	induced
T9164	19764-19766	IN	denotes	at
T9165	19767-19775	NN	denotes	5–30 min
T9166	19775-19776	-COMMA-	denotes	,
T9167	19777-19785	VB	denotes	followed
T9168	19786-19788	IN	denotes	by
T9169	19789-19791	FW	denotes	de
T9170	19792-19796	FW	denotes	novo
T9171	19797-19806	NN	denotes	synthesis
T9172	19807-19809	IN	denotes	at
T9173	19810-19816	NN	denotes	60 min
T9174	19817-19821	IN	denotes	with
T9175	19822-19826	JJ	denotes	full
T9176	19827-19840	NN	denotes	replenishment
T9177	19841-19843	IN	denotes	at
T9178	19844-19851	NN	denotes	240 min
T9179	19852-19854	IN	denotes	in
T9180	19855-19859	CC	denotes	both
T9181	19860-19872	JJ	denotes	Tat-positive
T9182	19873-19876	CC	denotes	and
T9183	19877-19885	JJ	denotes	negative
T9184	19886-19891	NN	denotes	cells
T9185	19892-19893	-LRB-	denotes	(
T9186	19893-19899	NN	denotes	Figure
T9187	19900-19902	NN	denotes	2B
T9188	19902-19903	-COMMA-	denotes	,
T9189	19904-19911	NN	denotes	cytosol
T9190	19911-19912	-RRB-	denotes	)
T9191	19914-19926	RB	denotes	Consistently
T9192	19926-19927	-COMMA-	denotes	,
T9193	19928-19931	DT	denotes	the
T9194	19932-19935	NN	denotes	IKK
T9195	19936-19946	NN	denotes	activation
T9196	19947-19954	VB	denotes	started
T9197	19955-19957	IN	denotes	at
T9198	19958-19963	NN	denotes	5 min
T9199	19963-19964	-COMMA-	denotes	,
T9200	19965-19968	CC	denotes	and
T9201	19969-19975	VB	denotes	turned
T9202	19976-19979	RP	denotes	off
T9203	19980-19982	IN	denotes	at
T9204	19983-19989	CD	denotes	30 min
T9205	19990-20003	RB	denotes	independently
T9206	20004-20006	IN	denotes	of
T9207	20007-20010	DT	denotes	the
T9208	20011-20019	NN	denotes	presence
T9209	20020-20022	IN	denotes	of
T9210	20023-20026	NN	denotes	Tat
T9211	20027-20028	-LRB-	denotes	(
T9212	20028-20034	NN	denotes	Figure
T9213	20035-20037	NN	denotes	2C
T9214	20037-20038	-RRB-	denotes	)
T9215	20040-20045	DT	denotes	These
T9216	20046-20053	NN	denotes	results
T9217	20054-20063	VB	denotes	indicated
T9218	20064-20068	IN	denotes	that
T9219	20069-20072	NN	denotes	Tat
T9220	20073-20081	VB	denotes	enhanced
T9221	20082-20085	DT	denotes	the
T9222	20086-20091	NN	denotes	NF-κB
T9223	20092-20100	NN	denotes	activity
T9224	20101-20103	IN	denotes	in
T9225	20104-20117	JJ	denotes	un-stimulated
T9226	20118-20121	CC	denotes	and
T9227	20122-20136	JJ	denotes	PMA-stimulated
T9228	20137-20142	NN	denotes	cells
T9229	20143-20150	IN	denotes	without
T9230	20151-20160	VB	denotes	affecting
T9231	20161-20164	DT	denotes	the
T9232	20165-20168	NN	denotes	IKK
T9233	20169-20177	NN	denotes	activity
T9234	20178-20181	CC	denotes	and
T9235	20182-20187	JJ	denotes	IκB-α
T9236	20188-20195	NN	denotes	content
T9237	21813-21815	IN	denotes	As
T9238	21816-21818	PRP	denotes	we
T9239	21819-21829	RB	denotes	previously
T9240	21830-21835	VB	denotes	found
T9241	21836-21840	IN	denotes	that
T9242	21841-21844	NN	denotes	Tat
T9243	21845-21850	VB	denotes	binds
T9244	21851-21853	TO	denotes	to
T9245	21854-21857	DT	denotes	the
T9246	21858-21863	JJ	denotes	sixth
T9247	21864-21871	NN	denotes	ankyrin
T9248	21872-21874	IN	denotes	of
T9249	21875-21880	NN	denotes	IκB-α
T9250	21881-21882	-LRB-	denotes	(
T9251	21882-21887	CD	denotes	50,51
T9252	21887-21888	-RRB-	denotes	)
T9253	21888-21889	-COMMA-	denotes	,
T9254	21890-21892	PRP	denotes	we
T9255	21893-21899	VB	denotes	tested
T9256	21900-21907	IN	denotes	whether
T9257	21908-21911	NN	denotes	Tat
T9258	21912-21924	VB	denotes	counteracted
T9259	21925-21928	DT	denotes	the
T9260	21929-21939	NN	denotes	generation
T9261	21940-21942	IN	denotes	of
T9262	21943-21946	DT	denotes	the
T9263	21947-21958	NN	denotes	IκB-α/NF-κB
T9264	21959-21966	NN	denotes	complex
T9265	21968-21970	TO	denotes	To
T9266	21971-21975	DT	denotes	this
T9267	21976-21979	NN	denotes	end
T9268	21979-21980	-COMMA-	denotes	,
T9269	21981-21984	DT	denotes	the
T9270	21985-21996	NN	denotes	association
T9271	21997-21999	IN	denotes	of
T9272	22000-22005	NN	denotes	IκB-α
T9273	22006-22010	IN	denotes	with
T9274	22011-22014	NN	denotes	p65
T9275	22015-22018	VB	denotes	was
T9276	22019-22027	VB	denotes	analysed
T9277	22028-22030	FW	denotes	in
T9278	22031-22035	FW	denotes	vivo
T9279	22036-22038	IN	denotes	at
T9280	22039-22040	CD	denotes	0
T9281	22041-22044	CC	denotes	and
T9282	22045-22052	NN	denotes	240 min
T9283	22053-22058	IN	denotes	after
T9284	22059-22070	JJ	denotes	short-pulse
T9285	22071-22074	NN	denotes	PMA
T9286	22074-22075	-COMMA-	denotes	,
T9287	22076-22081	WDT	denotes	which
T9288	22082-22094	VB	denotes	corresponded
T9289	22095-22097	TO	denotes	to
T9290	22098-22101	DT	denotes	the
T9291	22102-22106	NN	denotes	time
T9292	22107-22109	IN	denotes	of
T9293	22110-22123	JJ	denotes	un-stimulated
T9294	22124-22133	NN	denotes	condition
T9295	22134-22137	CC	denotes	and
T9296	22138-22153	JJ	denotes	post-activation
T9297	22154-22156	FW	denotes	de
T9298	22157-22161	FW	denotes	novo
T9299	22162-22171	NN	denotes	synthesis
T9300	22172-22174	IN	denotes	of
T9301	22175-22180	NN	denotes	IκB-α
T9302	22180-22181	-COMMA-	denotes	,
T9303	22182-22194	RB	denotes	respectively
T9304	22196-22217	NN	denotes	Coimmunoprecipitation
T9305	22218-22220	IN	denotes	of
T9306	22221-22226	NN	denotes	IκB-α
T9307	22227-22231	IN	denotes	with
T9308	22232-22235	NN	denotes	p65
T9309	22236-22239	VB	denotes	was
T9310	22240-22248	VB	denotes	detected
T9311	22249-22251	IN	denotes	at
T9312	22252-22254	CD	denotes	0-
T9313	22255-22258	CC	denotes	and
T9314	22259-22266	NN	denotes	240-min
T9315	22267-22281	JJ	denotes	post-treatment
T9316	22282-22284	IN	denotes	in
T9317	22285-22297	JJ	denotes	Tat-negative
T9318	22298-22303	NN	denotes	cells
T9319	22304-22305	-LRB-	denotes	(
T9320	22305-22311	NN	denotes	Figure
T9321	22312-22314	NN	denotes	2D
T9322	22314-22315	-COMMA-	denotes	,
T9323	22316-22321	NN	denotes	lanes
T9324	22322-22323	CD	denotes	1
T9325	22324-22327	CC	denotes	and
T9326	22328-22329	CD	denotes	3
T9327	22329-22330	-RRB-	denotes	)
T9328	22330-22331	-COMMA-	denotes	,
T9329	22332-22335	CC	denotes	and
T9330	22336-22339	VB	denotes	was
T9331	22340-22346	VB	denotes	halved
T9332	22347-22349	IN	denotes	in
T9333	22350-22362	JJ	denotes	Tat-positive
T9334	22363-22368	NN	denotes	cells
T9335	22368-22369	-COMMA-	denotes	,
T9336	22370-22375	WRB	denotes	where
T9337	22376-22379	NN	denotes	Tat
T9338	22380-22400	VB	denotes	coimmunoprecipitated
T9339	22401-22405	IN	denotes	with
T9340	22406-22411	NN	denotes	IκB-α
T9341	22412-22413	-LRB-	denotes	(
T9342	22413-22419	NN	denotes	Figure
T9343	22420-22422	NN	denotes	2D
T9344	22422-22423	-COMMA-	denotes	,
T9345	22424-22429	NN	denotes	lanes
T9346	22430-22431	CD	denotes	2
T9347	22432-22435	CC	denotes	and
T9348	22436-22437	CD	denotes	4
T9349	22437-22438	-RRB-	denotes	)
T9350	22438-22439	-COMMA-	denotes	,
T9351	22440-22450	VB	denotes	indicating
T9352	22451-22455	IN	denotes	that
T9353	22456-22459	NN	denotes	Tat
T9354	22460-22468	VB	denotes	competed
T9355	22469-22472	DT	denotes	the
T9356	22473-22478	JJ	denotes	IκB-α
T9357	22479-22486	NN	denotes	binding
T9358	22487-22489	TO	denotes	to
T9359	22490-22493	NN	denotes	p65
T11613	22496-22499	NN	denotes	Tat
T11614	22500-22511	VB	denotes	counteracts
T11615	22512-22515	DT	denotes	the
T11616	22516-22521	JJ	denotes	IκB-α
T11617	22522-22532	NN	denotes	inhibition
T11618	22533-22535	IN	denotes	of
T11619	22536-22539	NN	denotes	p65
T11620	22540-22542	IN	denotes	by
T11621	22543-22552	VB	denotes	competing
T11622	22553-22556	DT	denotes	the
T11623	22557-22566	NN	denotes	repressor
T11624	22567-22574	NN	denotes	binding
T11625	22575-22577	PRP	denotes	We
T11626	22578-22585	RB	denotes	further
T11627	22586-22598	VB	denotes	investigated
T11628	22599-22602	DT	denotes	the
T11629	22603-22611	JJ	denotes	physical
T11630	22612-22623	NN	denotes	interaction
T11631	22624-22626	IN	denotes	of
T11632	22627-22630	NN	denotes	Tat
T11633	22631-22635	IN	denotes	with
T11634	22636-22641	NN	denotes	IκB-α
T11635	22642-22647	VB	denotes	using
T11636	22648-22650	FW	denotes	in
T11637	22651-22656	FW	denotes	vitro
T11638	22657-22667	VB	denotes	translated
T11639	22668-22676	NN	denotes	proteins
T11640	22677-22679	IN	denotes	in
T11641	22680-22701	NN	denotes	coimmunoprecipitation
T11642	22702-22708	NN	denotes	assays
T11643	22710-22713	IN	denotes	For
T11644	22714-22721	VB	denotes	mapping
T11645	22722-22725	DT	denotes	the
T11646	22726-22737	NN	denotes	interaction
T11647	22738-22745	NN	denotes	domains
T11648	22745-22746	-COMMA-	denotes	,
T11649	22747-22749	PRP	denotes	we
T11650	22750-22754	VB	denotes	used
T11651	22755-22758	DT	denotes	the
T11652	22759-22768	VB	denotes	following
T11653	22769-22772	NN	denotes	Tat
T11654	22773-22780	NN	denotes	mutants
T11655	22780-22781	-COLON-	denotes	:
T11656	22782-22785	NN	denotes	Tat
T11657	22786-22787	NNP	denotes	T
T11658	22787-22788	-COMMA-	denotes	,
T11659	22788-22789	NN	denotes	N
T11660	22789-22790	-LRB-	denotes	(
T11661	22790-22795	CD	denotes	23,24
T11662	22795-22796	-RRB-	denotes	)
T11663	22796-22797	NN	denotes	A
T11664	22797-22798	-COMMA-	denotes	,
T11665	22799-22802	NN	denotes	Tat
T11666	22803-22804	NN	denotes	C
T11667	22804-22805	-LRB-	denotes	(
T11668	22805-22813	CD	denotes	22,25,27
T11669	22813-22814	-RRB-	denotes	)
T11670	22814-22815	NN	denotes	A
T11671	22815-22816	-COMMA-	denotes	,
T11672	22817-22820	NN	denotes	Tat
T11673	22821-22822	NN	denotes	K
T11674	22822-22823	-LRB-	denotes	(
T11675	22823-22828	CD	denotes	50,51
T11676	22828-22829	-RRB-	denotes	)
T11677	22829-22830	NN	denotes	A
T11678	22831-22834	CC	denotes	and
T11679	22835-22838	NN	denotes	Tat
T11680	22839-22840	NN	denotes	R
T11681	22840-22841	-LRB-	denotes	(
T11682	22841-22846	CD	denotes	49–57
T11683	22846-22847	-RRB-	denotes	)
T11684	22847-22848	NN	denotes	A
T11685	22849-22850	-LRB-	denotes	(
T11686	22850-22856	NN	denotes	Figure
T11687	22857-22859	NN	denotes	3A
T11688	22859-22860	-RRB-	denotes	)
T11689	22862-22867	NN	denotes	IκB-α
T11690	22868-22888	VB	denotes	coimmunoprecipitated
T11691	22889-22893	IN	denotes	with
T11692	22894-22897	DT	denotes	all
T11693	22898-22901	NN	denotes	Tat
T11694	22902-22910	NN	denotes	proteins
T11695	22910-22911	-COMMA-	denotes	,
T11696	22912-22918	IN	denotes	except
T11697	22919-22922	NN	denotes	Tat
T11698	22923-22924	NN	denotes	R
T11699	22924-22925	-LRB-	denotes	(
T11700	22925-22930	CD	denotes	49–57
T11701	22930-22931	-RRB-	denotes	)
T11702	22931-22932	NN	denotes	A
T11703	22933-22934	-LRB-	denotes	(
T11704	22934-22940	NN	denotes	Figure
T11705	22941-22943	NN	denotes	3B
T11706	22943-22944	-RRB-	denotes	)
T11707	22946-22951	DT	denotes	These
T11708	22952-22959	NN	denotes	results
T11709	22960-22969	VB	denotes	indicated
T11710	22970-22974	IN	denotes	that
T11711	22975-22978	DT	denotes	the
T11712	22979-22992	JJ	denotes	arginine-rich
T11713	22993-22999	NN	denotes	domain
T11714	23000-23002	IN	denotes	of
T11715	23003-23006	NN	denotes	Tat
T11716	23007-23010	VB	denotes	was
T11717	23011-23019	VB	denotes	involved
T11718	23020-23022	IN	denotes	in
T11719	23023-23026	DT	denotes	the
T11720	23027-23034	NN	denotes	binding
T11721	23035-23037	TO	denotes	to
T11722	23038-23043	NN	denotes	IκB-α
T11723	23043-23044	-COMMA-	denotes	,
T11724	23045-23050	WDT	denotes	which
T11725	23051-23054	VB	denotes	was
T11726	23055-23065	JJ	denotes	consistent
T11727	23066-23070	IN	denotes	with
T11728	23071-23079	JJ	denotes	previous
T11729	23080-23092	NN	denotes	observations
T11730	23093-23094	-LRB-	denotes	(
T11731	23094-23099	CD	denotes	50,51
T11732	23099-23100	-RRB-	denotes	)
T11733	23102-23110	RB	denotes	Moreover
T11734	23110-23111	-COMMA-	denotes	,
T11735	23112-23115	NN	denotes	Tat
T11736	23116-23127	VB	denotes	associating
T11737	23128-23132	IN	denotes	with
T11738	23133-23138	NN	denotes	IκB-α
T11739	23139-23147	VB	denotes	competed
T11740	23148-23151	DT	denotes	the
T11741	23152-23159	NN	denotes	binding
T11742	23160-23162	IN	denotes	of
T11743	23163-23168	NN	denotes	IκB-α
T11744	23169-23171	TO	denotes	to
T11745	23172-23175	NN	denotes	p65
T11746	23176-23178	IN	denotes	in
T11747	23179-23180	DT	denotes	a
T11748	23181-23195	JJ	denotes	dose-dependent
T11749	23196-23202	NN	denotes	manner
T11750	23203-23204	-LRB-	denotes	(
T11751	23204-23210	NN	denotes	Figure
T11752	23211-23213	NN	denotes	3C
T11753	23213-23214	-COMMA-	denotes	,
T11754	23215-23220	NN	denotes	lanes
T11755	23221-23224	CD	denotes	3–5
T11756	23224-23225	-RRB-	denotes	)
T11757	23225-23226	-COMMA-	denotes	,
T11758	23227-23232	IN	denotes	while
T11759	23233-23236	NN	denotes	Tat
T11760	23237-23238	NN	denotes	R
T11761	23238-23239	-LRB-	denotes	(
T11762	23239-23244	CD	denotes	49–57
T11763	23244-23245	-RRB-	denotes	)
T11764	23245-23246	NN	denotes	A
T11765	23246-23247	-COMMA-	denotes	,
T11766	23248-23253	WDT	denotes	which
T11767	23254-23260	VB	denotes	lacked
T11768	23261-23264	DT	denotes	the
T11769	23265-23272	VB	denotes	binding
T11770	23273-23277	NN	denotes	site
T11771	23278-23281	IN	denotes	for
T11772	23282-23287	NN	denotes	IκB-α
T11773	23287-23288	-COMMA-	denotes	,
T11774	23289-23292	VB	denotes	did
T11775	23293-23296	RB	denotes	not
T11776	23297-23306	VB	denotes	associate
T11777	23307-23311	IN	denotes	with
T11778	23312-23317	NN	denotes	IκB-α
T11779	23318-23321	CC	denotes	and
T11780	23322-23325	VB	denotes	did
T11781	23326-23329	RB	denotes	not
T11782	23330-23337	VB	denotes	compete
T11783	23338-23341	DT	denotes	the
T11784	23342-23349	NN	denotes	binding
T11785	23350-23352	IN	denotes	of
T11786	23353-23358	NN	denotes	IκB-α
T11787	23359-23361	TO	denotes	to
T11788	23362-23365	NN	denotes	p65
T11789	23366-23367	-LRB-	denotes	(
T11790	23367-23373	NN	denotes	Figure
T11791	23374-23376	NN	denotes	3C
T11792	23376-23377	-COMMA-	denotes	,
T11793	23378-23383	NN	denotes	lanes
T11794	23384-23387	CD	denotes	6–8
T11795	23387-23388	-RRB-	denotes	)
T11796	25484-25488	RB	denotes	Next
T11797	25488-25489	-COMMA-	denotes	,
T11798	25490-25492	PRP	denotes	we
T11799	25493-25502	VB	denotes	evaluated
T11800	25503-25510	IN	denotes	whether
T11801	25511-25514	NN	denotes	Tat
T11802	25515-25527	VB	denotes	counteracted
T11803	25528-25531	DT	denotes	the
T11804	25532-25546	JJ	denotes	IκB-α-mediated
T11805	25547-25557	NN	denotes	inhibition
T11806	25558-25560	IN	denotes	of
T11807	25561-25564	NN	denotes	p65
T11808	25565-25572	NN	denotes	binding
T11809	25573-25575	TO	denotes	to
T11810	25576-25579	NN	denotes	DNA
T11811	25580-25582	IN	denotes	by
T11812	25583-25593	VB	denotes	incubating
T11813	25594-25596	FW	denotes	in
T11814	25597-25602	FW	denotes	vitro
T11815	25603-25613	VB	denotes	translated
T11816	25614-25617	NN	denotes	p65
T11817	25618-25621	CC	denotes	and
T11818	25622-25627	NN	denotes	IκB-α
T11819	25628-25636	NN	denotes	proteins
T11820	25637-25641	IN	denotes	with
T11821	25642-25645	DT	denotes	the
T11822	25646-25651	NN	denotes	NF-κB
T11823	25652-25657	NN	denotes	probe
T11824	25658-25660	IN	denotes	in
T11825	25661-25669	NN	denotes	presence
T11826	25670-25672	CC	denotes	or
T11827	25673-25680	NN	denotes	absence
T11828	25681-25683	IN	denotes	of
T11829	25684-25687	NN	denotes	Tat
T11830	25688-25696	VB	denotes	followed
T11831	25697-25699	IN	denotes	by
T11832	25700-25704	NN	denotes	EMSA
T11833	25706-25709	DT	denotes	The
T11834	25710-25713	NN	denotes	p65
T11835	25714-25717	NN	denotes	DNA
T11836	25718-25725	NN	denotes	binding
T11837	25726-25734	NN	denotes	activity
T11838	25735-25738	VB	denotes	was
T11839	25739-25748	VB	denotes	inhibited
T11840	25749-25751	IN	denotes	by
T11841	25752-25757	NN	denotes	IκB-α
T11842	25758-25761	CC	denotes	and
T11843	25762-25770	VB	denotes	restored
T11844	25771-25773	IN	denotes	in
T11845	25774-25775	DT	denotes	a
T11846	25776-25790	JJ	denotes	dose-dependent
T11847	25791-25797	NN	denotes	manner
T11848	25798-25800	IN	denotes	by
T11849	25801-25810	JJ	denotes	wild-type
T11850	25811-25814	NN	denotes	Tat
T11851	25814-25815	-COMMA-	denotes	,
T11852	25816-25819	CC	denotes	and
T11853	25820-25823	RB	denotes	not
T11854	25824-25826	IN	denotes	by
T11855	25827-25830	NN	denotes	Tat
T11856	25831-25832	NN	denotes	R
T11857	25832-25833	-LRB-	denotes	(
T11858	25833-25838	CD	denotes	49–57
T11859	25838-25839	-RRB-	denotes	)
T11860	25840-25841	-LRB-	denotes	(
T11861	25841-25847	NN	denotes	Figure
T11862	25848-25850	NN	denotes	3D
T11863	25850-25851	-RRB-	denotes	)
T11864	25853-25860	RB	denotes	Further
T11865	25860-25861	-COMMA-	denotes	,
T11866	25862-25864	PRP	denotes	we
T11867	25865-25873	VB	denotes	analysed
T11868	25874-25877	DT	denotes	the
T11869	25878-25881	NN	denotes	Tat
T11870	25882-25888	NN	denotes	effect
T11871	25889-25891	IN	denotes	on
T11872	25892-25895	DT	denotes	the
T11873	25896-25901	JJ	denotes	IκB-α
T11874	25902-25912	NN	denotes	repression
T11875	25913-25915	IN	denotes	of
T11876	25916-25919	NN	denotes	p65
T11877	25920-25935	JJ	denotes	transcriptional
T11878	25936-25944	NN	denotes	activity
T11879	25945-25947	IN	denotes	by
T11880	25948-25960	VB	denotes	transfecting
T11881	25961-25977	NN	denotes	p50−/−p65−/−MEFs
T11882	25978-25982	IN	denotes	with
T11883	25983-25986	DT	denotes	the
T11884	25987-25996	NN	denotes	NF-κB-Luc
T11885	25997-26005	NN	denotes	reporter
T11886	26006-26014	RB	denotes	together
T11887	26015-26019	IN	denotes	with
T11888	26020-26030	NN	denotes	expression
T11889	26031-26038	NN	denotes	vectors
T11890	26039-26041	IN	denotes	of
T11891	26042-26045	NN	denotes	p65
T11892	26046-26049	CC	denotes	and
T11893	26050-26055	NN	denotes	IκB-α
T11894	26055-26056	-COMMA-	denotes	,
T11895	26057-26059	IN	denotes	in
T11896	26060-26068	NN	denotes	presence
T11897	26069-26071	CC	denotes	or
T11898	26072-26079	NN	denotes	absence
T11899	26080-26082	IN	denotes	of
T11900	26083-26087	NNP	denotes	Tat.
T11901	26088-26093	NNP	denotes	IκB-α
T11902	26094-26103	VB	denotes	inhibited
T11903	26104-26107	DT	denotes	the
T11904	26108-26121	JJ	denotes	p65-dependent
T11905	26122-26132	NN	denotes	expression
T11906	26133-26135	IN	denotes	of
T11907	26136-26139	DT	denotes	the
T11908	26140-26150	NN	denotes	luciferase
T11909	26151-26155	NN	denotes	gene
T11910	26155-26156	-COMMA-	denotes	,
T11911	26157-26162	WDT	denotes	which
T11912	26163-26166	VB	denotes	was
T11913	26167-26175	VB	denotes	restored
T11914	26176-26178	IN	denotes	in
T11915	26179-26180	DT	denotes	a
T11916	26181-26195	JJ	denotes	dose-dependent
T11917	26196-26202	NN	denotes	manner
T11918	26203-26205	IN	denotes	by
T11919	26206-26215	JJ	denotes	wild-type
T11920	26216-26219	NN	denotes	Tat
T11921	26219-26220	-COMMA-	denotes	,
T11922	26221-26224	CC	denotes	and
T11923	26225-26228	RB	denotes	not
T11924	26229-26231	IN	denotes	by
T11925	26232-26235	NN	denotes	Tat
T11926	26236-26237	NN	denotes	R
T11927	26237-26238	-LRB-	denotes	(
T11928	26238-26243	CD	denotes	49–57
T11929	26243-26244	-RRB-	denotes	)
T11930	26244-26245	NN	denotes	A
T11931	26246-26247	-LRB-	denotes	(
T11932	26247-26253	NN	denotes	Figure
T11933	26254-26256	NN	denotes	3E
T11934	26256-26257	-RRB-	denotes	)
T11935	26259-26269	RB	denotes	Altogether
T11936	26270-26275	DT	denotes	these
T11937	26276-26283	NN	denotes	results
T11938	26284-26293	VB	denotes	indicated
T11939	26294-26298	IN	denotes	that
T11940	26299-26302	NN	denotes	Tat
T11941	26303-26314	VB	denotes	counteracts
T11942	26315-26318	DT	denotes	the
T11943	26319-26324	JJ	denotes	IκB-α
T11944	26325-26335	NN	denotes	repression
T11945	26336-26338	IN	denotes	of
T11946	26339-26342	DT	denotes	the
T11947	26343-26346	NN	denotes	p65
T11948	26347-26358	JJ	denotes	DNA-binding
T11949	26359-26362	CC	denotes	and
T11950	26363-26378	JJ	denotes	transcriptional
T11951	26379-26387	NN	denotes	activity
T11952	26388-26390	IN	denotes	by
T11953	26391-26402	VB	denotes	associating
T11954	26403-26407	IN	denotes	with
T11955	26408-26413	NN	denotes	IκB-α
T11956	26414-26417	CC	denotes	and
T11957	26418-26427	VB	denotes	competing
T11958	26428-26431	DT	denotes	the
T11959	26432-26441	NN	denotes	repressor
T11960	26442-26449	NN	denotes	binding
T11961	26450-26452	TO	denotes	to
T11962	26453-26456	NN	denotes	p65
T13335	26459-26462	NN	denotes	Tat
T13336	26463-26472	VB	denotes	increases
T13337	26473-26476	DT	denotes	the
T13338	26477-26480	NN	denotes	p65
T13339	26481-26489	NN	denotes	affinity
T13340	26490-26497	NN	denotes	binding
T13341	26498-26500	TO	denotes	to
T13342	26501-26504	NN	denotes	DNA
T13343	26505-26512	IN	denotes	through
T13344	26513-26524	NN	denotes	association
T13345	26525-26529	IN	denotes	with
T13346	26530-26533	NN	denotes	p65
T13347	26534-26536	PRP	denotes	We
T13348	26537-26543	VB	denotes	tested
T13349	26544-26551	IN	denotes	whether
T13350	26552-26555	NN	denotes	Tat
T13351	26556-26566	RB	denotes	physically
T13352	26567-26577	VB	denotes	interacted
T13353	26578-26582	IN	denotes	with
T13354	26583-26586	NN	denotes	p65
T13355	26588-26592	NN	denotes	HeLa
T13356	26593-26598	NN	denotes	cells
T13357	26599-26603	VB	denotes	were
T13358	26604-26615	VB	denotes	transfected
T13359	26616-26620	IN	denotes	with
T13360	26621-26624	DT	denotes	the
T13361	26625-26635	NN	denotes	expression
T13362	26636-26643	NN	denotes	vectors
T13363	26644-26646	IN	denotes	of
T13364	26647-26653	NN	denotes	HA-p65
T13365	26654-26657	CC	denotes	and
T13366	26658-26666	NN	denotes	FLAG-Tat
T13367	26666-26667	-COMMA-	denotes	,
T13368	26668-26671	CC	denotes	and
T13369	26672-26676	NN	denotes	24 h
T13370	26677-26682	JJ	denotes	later
T13371	26683-26686	NN	denotes	Tat
T13372	26687-26690	VB	denotes	was
T13373	26691-26709	VB	denotes	immunoprecipitated
T13374	26710-26714	IN	denotes	from
T13375	26715-26719	NN	denotes	cell
T13376	26720-26728	NN	denotes	extracts
T13377	26730-26733	DT	denotes	The
T13378	26734-26737	NN	denotes	p65
T13379	26738-26745	NN	denotes	protein
T13380	26746-26749	VB	denotes	was
T13381	26750-26770	VB	denotes	coimmunoprecipitated
T13382	26771-26775	IN	denotes	with
T13383	26776-26785	JJ	denotes	wild-type
T13384	26786-26789	NN	denotes	Tat
T13385	26790-26792	CC	denotes	or
T13386	26793-26796	DT	denotes	the
T13387	26797-26804	NN	denotes	mutants
T13388	26805-26808	NN	denotes	Tat
T13389	26809-26810	NN	denotes	T
T13390	26810-26811	-COMMA-	denotes	,
T13391	26811-26812	NN	denotes	N
T13392	26812-26813	-LRB-	denotes	(
T13393	26813-26818	CD	denotes	23,24
T13394	26818-26819	-RRB-	denotes	)
T13395	26819-26820	NN	denotes	A
T13396	26820-26821	-COMMA-	denotes	,
T13397	26822-26825	NN	denotes	Tat
T13398	26826-26827	NN	denotes	K
T13399	26827-26828	-LRB-	denotes	(
T13400	26828-26833	CD	denotes	50,51
T13401	26833-26834	-RRB-	denotes	)
T13402	26834-26835	NN	denotes	A
T13403	26836-26839	CC	denotes	and
T13404	26840-26843	NN	denotes	Tat
T13405	26844-26845	NN	denotes	R
T13406	26845-26846	-LRB-	denotes	(
T13407	26846-26851	CD	denotes	49–57
T13408	26851-26852	-RRB-	denotes	)
T13409	26852-26853	NN	denotes	A
T13410	26854-26856	IN	denotes	at
T13411	26857-26864	JJ	denotes	similar
T13412	26865-26871	NN	denotes	levels
T13413	26871-26872	-COMMA-	denotes	,
T13414	26873-26878	IN	denotes	while
T13415	26879-26880	DT	denotes	a
T13416	26881-26892	JJ	denotes	significant
T13417	26893-26901	NN	denotes	decrease
T13418	26902-26904	IN	denotes	in
T13419	26905-26926	NN	denotes	coimmunoprecipitation
T13420	26927-26930	VB	denotes	was
T13421	26931-26939	VB	denotes	observed
T13422	26940-26943	IN	denotes	for
T13423	26944-26947	NN	denotes	Tat
T13424	26948-26949	NN	denotes	C
T13425	26949-26950	-LRB-	denotes	(
T13426	26950-26958	CD	denotes	22,25,27
T13427	26958-26959	-RRB-	denotes	)
T13428	26959-26960	NN	denotes	A
T13429	26961-26962	-LRB-	denotes	(
T13430	26962-26968	NN	denotes	Figure
T13431	26969-26971	NN	denotes	4A
T13432	26971-26972	-RRB-	denotes	)
T13433	26974-26986	RB	denotes	Consistently
T13434	26986-26987	-COMMA-	denotes	,
T13435	26988-26996	NNP	denotes	GST-pull
T13436	26997-27001	IN	denotes	down
T13437	27002-27004	IN	denotes	of
T13438	27005-27007	FW	denotes	in
T13439	27008-27013	FW	denotes	vitro
T13440	27014-27024	VB	denotes	translated
T13441	27025-27033	NN	denotes	proteins
T13442	27034-27040	VB	denotes	showed
T13443	27041-27044	DT	denotes	the
T13444	27045-27051	JJ	denotes	direct
T13445	27052-27059	NN	denotes	binding
T13446	27060-27062	IN	denotes	of
T13447	27063-27066	NN	denotes	p65
T13448	27067-27069	TO	denotes	to
T13449	27070-27079	JJ	denotes	wild-type
T13450	27080-27083	NN	denotes	Tat
T13451	27083-27084	-COMMA-	denotes	,
T13452	27085-27087	CC	denotes	or
T13453	27088-27091	DT	denotes	the
T13454	27092-27099	NN	denotes	mutants
T13455	27100-27103	NN	denotes	Tat
T13456	27104-27105	NN	denotes	T
T13457	27105-27106	-COMMA-	denotes	,
T13458	27106-27107	NN	denotes	N
T13459	27107-27108	-LRB-	denotes	(
T13460	27108-27113	CD	denotes	23,24
T13461	27113-27114	-RRB-	denotes	)
T13462	27114-27115	NN	denotes	A
T13463	27115-27116	-COMMA-	denotes	,
T13464	27117-27120	NN	denotes	Tat
T13465	27121-27122	NN	denotes	K
T13466	27122-27123	-LRB-	denotes	(
T13467	27123-27128	CD	denotes	50,51
T13468	27128-27129	-RRB-	denotes	)
T13469	27129-27130	NN	denotes	A
T13470	27131-27134	CC	denotes	and
T13471	27135-27138	NN	denotes	Tat
T13472	27139-27140	NN	denotes	R
T13473	27140-27141	-LRB-	denotes	(
T13474	27141-27146	CD	denotes	49–57
T13475	27146-27147	-RRB-	denotes	)
T13476	27147-27148	NN	denotes	A
T13477	27148-27149	-COMMA-	denotes	,
T13478	27150-27153	CC	denotes	and
T13479	27154-27158	NN	denotes	lack
T13480	27159-27161	IN	denotes	of
T13481	27162-27169	VB	denotes	binding
T13482	27170-27172	TO	denotes	to
T13483	27173-27176	NN	denotes	Tat
T13484	27177-27178	NN	denotes	C
T13485	27178-27179	-LRB-	denotes	(
T13486	27179-27187	CD	denotes	22,25,27
T13487	27187-27188	-RRB-	denotes	)
T13488	27188-27189	NN	denotes	A
T13489	27190-27191	-LRB-	denotes	(
T13490	27191-27197	NN	denotes	Figure
T13491	27198-27200	NN	denotes	4B
T13492	27200-27201	-RRB-	denotes	)
T13493	27203-27208	DT	denotes	These
T13494	27209-27216	NN	denotes	results
T13495	27217-27226	VB	denotes	indicated
T13496	27227-27231	IN	denotes	that
T13497	27232-27235	NN	denotes	Tat
T13498	27236-27246	VB	denotes	associated
T13499	27247-27251	IN	denotes	with
T13500	27252-27255	NN	denotes	p65
T13501	27256-27263	IN	denotes	through
T13502	27264-27267	DT	denotes	the
T13503	27268-27281	JJ	denotes	cysteine-rich
T13504	27282-27288	NN	denotes	domain
T13505	27289-27292	CC	denotes	and
T13506	27293-27302	NN	denotes	ruled-out
T13507	27303-27306	DT	denotes	the
T13508	27307-27318	NN	denotes	requirement
T13509	27319-27321	IN	denotes	of
T13510	27322-27330	VB	denotes	bridging
T13511	27331-27339	NN	denotes	proteins
T13512	27340-27342	TO	denotes	to
T13513	27343-27350	VB	denotes	mediate
T13514	27351-27355	JJ	denotes	such
T13515	27356-27367	NN	denotes	interaction
T13516	29093-29095	TO	denotes	To
T13517	29096-29104	VB	denotes	identify
T13518	29105-29108	DT	denotes	the
T13519	29109-29112	NN	denotes	p65
T13520	29113-29119	NN	denotes	domain
T13521	29120-29128	VB	denotes	required
T13522	29129-29132	IN	denotes	for
T13523	29133-29140	VB	denotes	binding
T13524	29141-29143	TO	denotes	to
T13525	29144-29147	NN	denotes	Tat
T13526	29147-29148	-COMMA-	denotes	,
T13527	29149-29153	NN	denotes	HeLa
T13528	29154-29159	NN	denotes	cells
T13529	29160-29164	VB	denotes	were
T13530	29165-29176	VB	denotes	transfected
T13531	29177-29181	IN	denotes	with
T13532	29182-29190	NN	denotes	FLAG-Tat
T13533	29191-29199	RB	denotes	together
T13534	29200-29204	IN	denotes	with
T13535	29205-29211	NN	denotes	HA-p65
T13536	29212-29219	NN	denotes	mutants
T13537	29219-29220	-COMMA-	denotes	,
T13538	29221-29226	WDT	denotes	which
T13539	29227-29231	VB	denotes	were
T13540	29232-29239	VB	denotes	deleted
T13541	29240-29242	IN	denotes	of
T13542	29243-29246	DT	denotes	the
T13543	29247-29262	NN	denotes	transactivation
T13544	29263-29269	NN	denotes	domain
T13545	29270-29271	-LRB-	denotes	[
T13546	29271-29276	NN	denotes	p65ΔC
T13547	29277-29278	-LRB-	denotes	(
T13548	29278-29283	CD	denotes	1–318
T13549	29283-29284	-RRB-	denotes	)
T13550	29284-29285	-RRB-	denotes	]
T13551	29285-29286	-COMMA-	denotes	,
T13552	29287-29289	CC	denotes	or
T13553	29290-29293	DT	denotes	the
T13554	29294-29297	NN	denotes	RHD
T13555	29298-29299	-LRB-	denotes	[
T13556	29299-29304	NN	denotes	p65ΔN
T13557	29305-29306	-LRB-	denotes	(
T13558	29306-29313	CD	denotes	122–551
T13559	29313-29314	-RRB-	denotes	)
T13560	29314-29315	-RRB-	denotes	]
T13561	29316-29317	-LRB-	denotes	(
T13562	29317-29323	NN	denotes	Figure
T13563	29324-29326	NN	denotes	4C
T13564	29326-29327	-RRB-	denotes	)
T13565	29329-29332	NN	denotes	Tat
T13566	29333-29353	VB	denotes	coimmunoprecipitated
T13567	29354-29358	IN	denotes	with
T13568	29359-29364	NN	denotes	p65ΔC
T13569	29365-29366	-LRB-	denotes	(
T13570	29366-29371	CD	denotes	1–318
T13571	29371-29372	-RRB-	denotes	)
T13572	29372-29373	-COMMA-	denotes	,
T13573	29374-29377	CC	denotes	and
T13574	29378-29381	RB	denotes	not
T13575	29382-29386	IN	denotes	with
T13576	29387-29392	NN	denotes	p65ΔN
T13577	29393-29394	-LRB-	denotes	(
T13578	29394-29401	CD	denotes	122–551
T13579	29401-29402	-RRB-	denotes	)
T13580	29403-29404	-LRB-	denotes	(
T13581	29404-29410	NN	denotes	Figure
T13582	29411-29413	NN	denotes	4D
T13583	29413-29414	-RRB-	denotes	)
T13584	29414-29415	-COMMA-	denotes	,
T13585	29416-29426	VB	denotes	indicating
T13586	29427-29431	IN	denotes	that
T13587	29432-29435	DT	denotes	the
T13588	29436-29439	NN	denotes	RHD
T13589	29440-29442	IN	denotes	of
T13590	29443-29446	NN	denotes	p65
T13591	29447-29450	VB	denotes	was
T13592	29451-29459	VB	denotes	involved
T13593	29460-29462	IN	denotes	in
T13594	29463-29466	DT	denotes	the
T13595	29467-29475	JJ	denotes	physical
T13596	29476-29487	NN	denotes	interaction
T13597	29488-29492	IN	denotes	with
T13598	29493-29496	NN	denotes	Tat
T13599	29498-29500	TO	denotes	To
T13600	29501-29508	VB	denotes	address
T13601	29509-29512	DT	denotes	the
T13602	29513-29519	NN	denotes	effect
T13603	29520-29522	IN	denotes	of
T13604	29523-29526	NN	denotes	Tat
T13605	29527-29529	IN	denotes	on
T13606	29530-29533	DT	denotes	the
T13607	29534-29537	NN	denotes	p65
T13608	29538-29549	JJ	denotes	DNA-binding
T13609	29550-29558	NN	denotes	affinity
T13610	29558-29559	-COMMA-	denotes	,
T13611	29560-29568	VB	denotes	purified
T13612	29569-29580	JJ	denotes	recombinant
T13613	29581-29584	NN	denotes	p65
T13614	29585-29592	NN	denotes	protein
T13615	29593-29596	VB	denotes	was
T13616	29597-29606	VB	denotes	incubated
T13617	29607-29611	IN	denotes	with
T13618	29612-29629	NN	denotes	32P-labeled-NF-κB
T13619	29630-29645	JJ	denotes	double-stranded
T13620	29646-29661	NN	denotes	oligonucleotide
T13621	29662-29664	IN	denotes	in
T13622	29665-29673	NN	denotes	presence
T13623	29674-29676	CC	denotes	or
T13624	29677-29684	NN	denotes	absence
T13625	29685-29687	IN	denotes	of
T13626	29688-29697	JJ	denotes	wild-type
T13627	29698-29701	NN	denotes	Tat
T13628	29702-29704	CC	denotes	or
T13629	29705-29708	NN	denotes	Tat
T13630	29709-29710	NN	denotes	C
T13631	29710-29711	-LRB-	denotes	(
T13632	29711-29719	CD	denotes	22,25,27
T13633	29719-29720	-RRB-	denotes	)
T13634	29720-29721	NN	denotes	A
T13635	29721-29722	-COMMA-	denotes	,
T13636	29723-29726	CC	denotes	and
T13637	29727-29730	NN	denotes	p65
T13638	29731-29742	NN	denotes	DNA-binding
T13639	29743-29746	VB	denotes	was
T13640	29747-29755	VB	denotes	analysed
T13641	29756-29758	IN	denotes	by
T13642	29759-29763	NN	denotes	EMSA
T13643	29765-29772	IN	denotes	Whereas
T13644	29773-29782	JJ	denotes	wild-type
T13645	29783-29786	NN	denotes	Tat
T13646	29787-29800	RB	denotes	significantly
T13647	29801-29810	VB	denotes	increased
T13648	29811-29814	DT	denotes	the
T13649	29815-29822	NN	denotes	binding
T13650	29823-29825	IN	denotes	of
T13651	29826-29829	NN	denotes	p65
T13652	29830-29832	TO	denotes	to
T13653	29833-29836	NN	denotes	DNA
T13654	29836-29837	-COMMA-	denotes	,
T13655	29838-29841	DT	denotes	the
T13656	29842-29848	JJ	denotes	mutant
T13657	29849-29852	NN	denotes	Tat
T13658	29853-29854	NN	denotes	C
T13659	29854-29855	-LRB-	denotes	(
T13660	29855-29863	CD	denotes	22,25,27
T13661	29863-29864	-RRB-	denotes	)
T13662	29864-29865	NN	denotes	A
T13663	29865-29866	-COMMA-	denotes	,
T13664	29867-29874	VB	denotes	lacking
T13665	29875-29878	DT	denotes	the
T13666	29879-29886	VB	denotes	binding
T13667	29887-29891	NN	denotes	site
T13668	29892-29895	IN	denotes	for
T13669	29896-29899	NN	denotes	p65
T13670	29899-29900	-COMMA-	denotes	,
T13671	29901-29904	VB	denotes	was
T13672	29905-29916	JJ	denotes	ineffective
T13673	29917-29918	-LRB-	denotes	(
T13674	29918-29924	NN	denotes	Figure
T13675	29925-29927	NN	denotes	4E
T13676	29927-29928	-RRB-	denotes	)
T13677	29930-29933	DT	denotes	The
T13678	29934-29937	NN	denotes	p65
T13679	29938-29945	NN	denotes	binding
T13680	29946-29948	TO	denotes	to
T13681	29949-29952	NN	denotes	DNA
T13682	29953-29956	VB	denotes	was
T13683	29957-29966	VB	denotes	evaluated
T13684	29967-29969	IN	denotes	by
T13685	29970-29981	NN	denotes	competition
T13686	29982-29986	IN	denotes	with
T13687	29987-29997	VB	denotes	increasing
T13688	29998-30005	NN	denotes	amounts
T13689	30006-30008	IN	denotes	of
T13690	30009-30013	JJ	denotes	cold
T13691	30014-30024	NN	denotes	competitor
T13692	30025-30030	NN	denotes	NF-κB
T13693	30031-30046	NN	denotes	oligonucleotide
T13694	30046-30047	-COMMA-	denotes	,
T13695	30048-30053	WDT	denotes	which
T13696	30054-30060	VB	denotes	showed
T13697	30061-30065	IN	denotes	that
T13698	30066-30069	NN	denotes	Tat
T13699	30070-30083	RB	denotes	significantly
T13700	30084-30092	VB	denotes	enhanced
T13701	30093-30096	DT	denotes	the
T13702	30097-30100	NN	denotes	p65
T13703	30101-30109	NN	denotes	affinity
T13704	30110-30117	NN	denotes	binding
T13705	30118-30120	TO	denotes	to
T13706	30121-30124	NN	denotes	DNA
T13707	30125-30126	-LRB-	denotes	(
T13708	30126-30132	NN	denotes	Figure
T13709	30133-30135	NN	denotes	4E
T13710	30136-30139	CC	denotes	and
T13711	30140-30141	NN	denotes	F
T13712	30141-30142	-RRB-	denotes	)
T15237	30145-30148	NN	denotes	Tat
T15238	30149-30158	VB	denotes	activates
T15239	30159-30162	DT	denotes	the
T15240	30163-30176	JJ	denotes	p65-dependent
T15241	30177-30187	NN	denotes	expression
T15242	30188-30190	IN	denotes	of
T15243	30191-30207	JJ	denotes	NF-κB-responsive
T15244	30208-30213	NN	denotes	genes
T15245	30213-30214	-COMMA-	denotes	,
T15246	30215-30223	VB	denotes	occupies
T15247	30224-30227	DT	denotes	the
T15248	30228-30233	NN	denotes	NF-κB
T15249	30234-30243	NN	denotes	enhancers
T15250	30244-30247	CC	denotes	and
T15251	30248-30256	VB	denotes	promotes
T15252	30257-30260	DT	denotes	the
T15253	30261-30264	NN	denotes	p65
T15254	30265-30276	NN	denotes	recruitment
T15255	30277-30281	IN	denotes	with
T15256	30282-30287	NN	denotes	IκB-α
T15257	30288-30300	NN	denotes	displacement
T15258	30301-30303	PRP	denotes	We
T15259	30304-30312	VB	denotes	analysed
T15260	30313-30316	DT	denotes	the
T15261	30317-30323	NN	denotes	action
T15262	30324-30326	IN	denotes	of
T15263	30327-30330	NN	denotes	Tat
T15264	30331-30333	IN	denotes	on
T15265	30334-30337	DT	denotes	the
T15266	30338-30348	NN	denotes	expression
T15267	30349-30351	IN	denotes	of
T15268	30352-30368	JJ	denotes	NF-κB-responsive
T15269	30369-30374	NN	denotes	genes
T15270	30375-30377	FW	denotes	in
T15271	30378-30382	FW	denotes	vivo
T15272	30384-30386	TO	denotes	To
T15273	30387-30391	DT	denotes	this
T15274	30392-30395	NN	denotes	end
T15275	30395-30396	-COMMA-	denotes	,
T15276	30397-30401	NN	denotes	HeLa
T15277	30402-30407	NN	denotes	cells
T15278	30408-30412	VB	denotes	were
T15279	30413-30424	VB	denotes	transfected
T15280	30425-30429	IN	denotes	with
T15281	30430-30438	NN	denotes	FLAG-Tat
T15282	30438-30439	-COMMA-	denotes	,
T15283	30440-30448	NN	denotes	FLAG-Tat
T15284	30449-30450	NN	denotes	C
T15285	30450-30451	-LRB-	denotes	(
T15286	30451-30459	CD	denotes	22,25,27
T15287	30459-30460	-RRB-	denotes	)
T15288	30460-30461	NN	denotes	A
T15289	30462-30464	CC	denotes	or
T15290	30465-30473	NN	denotes	FLAG-Tat
T15291	30474-30475	NN	denotes	R
T15292	30475-30476	-LRB-	denotes	(
T15293	30476-30481	CD	denotes	49–57
T15294	30481-30482	-RRB-	denotes	)
T15295	30482-30483	NN	denotes	A
T15296	30483-30484	-COMMA-	denotes	,
T15297	30485-30488	CC	denotes	and
T15298	30489-30493	NN	denotes	48 h
T15299	30494-30499	RB	denotes	later
T15300	30500-30503	DT	denotes	the
T15301	30504-30514	NN	denotes	expression
T15302	30515-30517	IN	denotes	of
T15303	30518-30519	DT	denotes	a
T15304	30520-30526	NN	denotes	number
T15305	30527-30529	IN	denotes	of
T15306	30530-30545	JJ	denotes	NF-κB-dependent
T15307	30546-30551	NN	denotes	genes
T15308	30552-30555	VB	denotes	was
T15309	30556-30564	VB	denotes	analysed
T15310	30565-30567	IN	denotes	by
T15311	30568-30577	JJ	denotes	real-time
T15312	30578-30581	NN	denotes	PCR
T15313	30583-30586	NN	denotes	Tat
T15314	30587-30600	RB	denotes	significantly
T15315	30601-30610	VB	denotes	increased
T15316	30611-30614	DT	denotes	the
T15317	30615-30625	NN	denotes	expression
T15318	30626-30628	IN	denotes	of
T15319	30629-30635	NN	denotes	MIP-1α
T15320	30635-30636	-COMMA-	denotes	,
T15321	30637-30641	NN	denotes	CSF3
T15322	30641-30642	-COMMA-	denotes	,
T15323	30643-30646	NN	denotes	LTA
T15324	30646-30647	-COMMA-	denotes	,
T15325	30648-30654	NN	denotes	NFKBIA
T15326	30655-30658	CC	denotes	and
T15327	30659-30663	NN	denotes	TLR2
T15328	30663-30664	-COMMA-	denotes	,
T15329	30665-30670	IN	denotes	while
T15330	30671-30674	DT	denotes	the
T15331	30675-30682	NN	denotes	mutants
T15332	30683-30686	NN	denotes	Tat
T15333	30687-30688	NN	denotes	C
T15334	30688-30689	-LRB-	denotes	(
T15335	30689-30697	CD	denotes	22,25,27
T15336	30697-30698	-RRB-	denotes	)
T15337	30698-30699	NN	denotes	A
T15338	30700-30703	CC	denotes	and
T15339	30704-30707	NN	denotes	Tat
T15340	30708-30709	NN	denotes	R
T15341	30709-30710	-LRB-	denotes	(
T15342	30710-30715	CD	denotes	49–57
T15343	30715-30716	-RRB-	denotes	)
T15344	30716-30717	NN	denotes	A
T15345	30718-30722	VB	denotes	were
T15346	30723-30734	JJ	denotes	ineffective
T15347	30735-30736	-LRB-	denotes	(
T15348	30736-30742	NN	denotes	Figure
T15349	30743-30747	NN	denotes	5A–E
T15350	30747-30748	-RRB-	denotes	)
T15351	30750-30753	DT	denotes	The
T15352	30754-30767	JJ	denotes	Tat-dependent
T15353	30768-30783	NN	denotes	transactivation
T15354	30784-30786	IN	denotes	of
T15355	30787-30790	DT	denotes	the
T15356	30791-30806	JJ	denotes	NF-κB-dependent
T15357	30807-30812	NN	denotes	genes
T15358	30813-30816	VB	denotes	was
T15359	30817-30826	VB	denotes	abolished
T15360	30827-30831	WRB	denotes	when
T15361	30832-30837	NN	denotes	cells
T15362	30838-30842	VB	denotes	were
T15363	30843-30854	VB	denotes	transfected
T15364	30855-30859	IN	denotes	with
T15365	30860-30865	NN	denotes	siRNA
T15366	30866-30869	NN	denotes	p65
T15367	30869-30870	-COMMA-	denotes	,
T15368	30871-30881	VB	denotes	indicating
T15369	30882-30886	IN	denotes	that
T15370	30887-30890	DT	denotes	the
T15371	30891-30894	NN	denotes	Tat
T15372	30895-30910	JJ	denotes	transcriptional
T15373	30911-30921	NN	denotes	activation
T15374	30922-30925	VB	denotes	was
T15375	30926-30934	VB	denotes	mediated
T15376	30935-30937	IN	denotes	by
T15377	30938-30941	NN	denotes	p65
T15378	30942-30943	-LRB-	denotes	(
T15379	30943-30949	NN	denotes	Figure
T15380	30950-30954	NN	denotes	5A–E
T15381	30954-30955	-RRB-	denotes	)
T15382	30957-30968	RB	denotes	Differently
T15383	30968-30969	-COMMA-	denotes	,
T15384	30970-30973	DT	denotes	the
T15385	30974-30984	NN	denotes	expression
T15386	30985-30987	IN	denotes	of
T15387	30988-30991	DT	denotes	the
T15388	30992-31009	JJ	denotes	NF-κB-independent
T15389	31010-31015	NN	denotes	genes
T15390	31016-31021	NN	denotes	GAPDH
T15391	31022-31025	CC	denotes	and
T15392	31026-31030	NN	denotes	ACTB
T15393	31031-31034	VB	denotes	was
T15394	31035-31045	JJ	denotes	unaffected
T15395	31046-31048	IN	denotes	by
T15396	31049-31058	JJ	denotes	wild-type
T15397	31059-31062	CC	denotes	and
T15398	31063-31066	NN	denotes	Tat
T15399	31067-31074	NN	denotes	mutants
T15400	31075-31076	-LRB-	denotes	(
T15401	31076-31082	NN	denotes	Figure
T15402	31083-31085	NN	denotes	5F
T15403	31086-31089	CC	denotes	and
T15404	31090-31091	NN	denotes	G
T15405	31091-31092	-RRB-	denotes	)
T15406	31094-31099	DT	denotes	These
T15407	31100-31107	NN	denotes	results
T15408	31108-31112	VB	denotes	were
T15409	31113-31123	JJ	denotes	consistent
T15410	31124-31128	IN	denotes	with
T15411	31129-31132	DT	denotes	the
T15412	31133-31144	NN	denotes	requirement
T15413	31145-31147	IN	denotes	of
T15414	31148-31152	CC	denotes	both
T15415	31153-31156	DT	denotes	the
T15416	31157-31170	JJ	denotes	cysteine-rich
T15417	31171-31174	CC	denotes	and
T15418	31175-31188	JJ	denotes	arginine-rich
T15419	31189-31196	NN	denotes	domains
T15420	31197-31199	IN	denotes	of
T15421	31200-31203	NN	denotes	Tat
T15422	31204-31206	TO	denotes	to
T15423	31207-31214	VB	denotes	enhance
T15424	31215-31218	DT	denotes	the
T15425	31219-31224	NN	denotes	NF-κB
T15426	31225-31233	NN	denotes	activity
T15427	31233-31234	-COMMA-	denotes	,
T15428	31235-31245	VB	denotes	suggesting
T15429	31246-31250	IN	denotes	that
T15430	31251-31254	DT	denotes	the
T15431	31255-31268	NN	denotes	up-regulation
T15432	31269-31271	IN	denotes	of
T15433	31272-31288	JJ	denotes	NF-κB-responsive
T15434	31289-31294	NN	denotes	genes
T15435	31295-31297	IN	denotes	by
T15436	31298-31301	NN	denotes	Tat
T15437	31302-31307	MD	denotes	might
T15438	31308-31313	VB	denotes	occur
T15439	31314-31321	IN	denotes	through
T15440	31322-31330	JJ	denotes	physical
T15441	31331-31342	NN	denotes	interaction
T15442	31343-31345	IN	denotes	of
T15443	31346-31349	DT	denotes	the
T15444	31350-31355	JJ	denotes	viral
T15445	31356-31363	NN	denotes	protein
T15446	31364-31368	IN	denotes	with
T15447	31369-31372	NN	denotes	p65
T15448	31373-31376	CC	denotes	and
T15449	31377-31382	NN	denotes	IκB-α
T15450	32978-32984	NN	denotes	MIP-1α
T15451	32984-32985	-COMMA-	denotes	,
T15452	32986-32991	WDT	denotes	which
T15453	32992-32995	VB	denotes	was
T15454	32996-32999	DT	denotes	the
T15455	33000-33006	RB	denotes	mostly
T15456	33007-33016	VB	denotes	activated
T15457	33017-33021	NN	denotes	gene
T15458	33022-33024	IN	denotes	by
T15459	33025-33028	NN	denotes	Tat
T15460	33028-33029	-COMMA-	denotes	,
T15461	33030-33037	VB	denotes	encodes
T15462	33038-33041	IN	denotes	for
T15463	33042-33043	DT	denotes	a
T15464	33044-33053	NN	denotes	chemokine
T15465	33054-33058	WDT	denotes	that
T15466	33059-33067	VB	denotes	promotes
T15467	33068-33071	DT	denotes	the
T15468	33072-33083	NN	denotes	recruitment
T15469	33084-33086	IN	denotes	of
T15470	33087-33103	JJ	denotes	pro-inflammatory
T15471	33104-33109	NN	denotes	cells
T15472	33110-33111	-LRB-	denotes	(
T15473	33111-33113	CD	denotes	56
T15474	33113-33114	-RRB-	denotes	)
T15475	33116-33128	RB	denotes	Consistently
T15476	33129-33133	IN	denotes	with
T15477	33134-33137	PRP-DOLLAR-	denotes	our
T15478	33138-33146	NN	denotes	findings
T15479	33146-33147	-COMMA-	denotes	,
T15480	33148-33154	NN	denotes	MIP-1α
T15481	33155-33165	NN	denotes	expression
T15482	33166-33169	VB	denotes	was
T15483	33170-33179	VB	denotes	increased
T15484	33180-33182	IN	denotes	in
T15485	33183-33188	JJ	denotes	glial
T15486	33189-33194	NN	denotes	cells
T15487	33195-33198	CC	denotes	and
T15488	33199-33204	NN	denotes	lymph
T15489	33205-33210	NN	denotes	nodes
T15490	33211-33213	IN	denotes	of
T15491	33214-33218	NN	denotes	AIDS
T15492	33219-33227	NN	denotes	patients
T15493	33228-33229	-LRB-	denotes	(
T15494	33229-33234	CD	denotes	57,58
T15495	33234-33235	-RRB-	denotes	)
T15496	33235-33236	-COLON-	denotes	;
T15497	33237-33245	RB	denotes	moreover
T15498	33245-33246	-COMMA-	denotes	,
T15499	33247-33253	NN	denotes	MIP-1α
T15500	33254-33264	NN	denotes	production
T15501	33265-33268	VB	denotes	was
T15502	33269-33276	VB	denotes	induced
T15503	33277-33279	IN	denotes	by
T15504	33280-33285	NN	denotes	HIV-1
T15505	33286-33295	NN	denotes	infection
T15506	33296-33297	-LRB-	denotes	(
T15507	33297-33302	CD	denotes	59,60
T15508	33302-33303	-RRB-	denotes	)
T15509	33304-33307	CC	denotes	and
T15510	33308-33311	NN	denotes	Tat
T15511	33312-33319	NN	denotes	protein
T15512	33320-33321	-LRB-	denotes	(
T15513	33321-33329	CD	denotes	57,61,62
T15514	33329-33330	-RRB-	denotes	)
T15515	33332-33335	DT	denotes	The
T15516	33336-33351	JJ	denotes	transcriptional
T15517	33352-33362	NN	denotes	regulation
T15518	33363-33365	IN	denotes	of
T15519	33366-33372	NN	denotes	MIP-1α
T15520	33373-33376	VB	denotes	has
T15521	33377-33381	VB	denotes	been
T15522	33382-33388	RB	denotes	poorly
T15523	33389-33402	VB	denotes	characterized
T15524	33404-33416	JJ	denotes	Jaspar-based
T15525	33417-33425	NN	denotes	analysis
T15526	33426-33427	-LRB-	denotes	(
T15527	33427-33431	NN	denotes	http
T15528	33431-33432	-COLON-	denotes	:
T15529	33432-33453	LS	denotes	//jaspar.genereg.net/
T15530	33453-33454	-RRB-	denotes	)
T15531	33455-33464	VB	denotes	predicted
T15532	33465-33470	CD	denotes	three
T15533	33471-33479	JJ	denotes	putative
T15534	33480-33485	NN	denotes	NF-κB
T15535	33486-33495	NN	denotes	enhancers
T15536	33496-33498	IN	denotes	in
T15537	33499-33502	DT	denotes	the
T15538	33503-33511	JJ	denotes	proximal
T15539	33512-33520	NN	denotes	promoter
T15540	33521-33527	NN	denotes	region
T15541	33528-33530	IN	denotes	of
T15542	33531-33534	DT	denotes	the
T15543	33535-33541	NN	denotes	MIP-1α
T15544	33542-33546	NN	denotes	gene
T15545	33546-33547	-COLON-	denotes	:
T15546	33548-33554	NN	denotes	NF-κB1
T15547	33554-33555	-COMMA-	denotes	,
T15548	33556-33567	CD	denotes	−1023/−1014
T15549	33568-33579	NN	denotes	nucleotides
T15550	33579-33580	-COLON-	denotes	;
T15551	33581-33587	NN	denotes	NF-κB2
T15552	33587-33588	-COMMA-	denotes	,
T15553	33589-33598	CD	denotes	−661/−652
T15554	33599-33610	NN	denotes	nucleotides
T15555	33610-33611	-COLON-	denotes	;
T15556	33612-33618	NN	denotes	NF-κB3
T15557	33618-33619	-COMMA-	denotes	,
T15558	33620-33629	CD	denotes	+370/+379
T15559	33630-33641	NN	denotes	nucleotides
T15560	33642-33643	-LRB-	denotes	(
T15561	33643-33656	JJ	denotes	Supplementary
T15562	33657-33663	NN	denotes	Figure
T15563	33664-33667	NN	denotes	S3A
T15564	33667-33668	-RRB-	denotes	)
T15565	33670-33672	TO	denotes	To
T15566	33673-33681	VB	denotes	validate
T15567	33682-33685	DT	denotes	the
T15568	33686-33695	VB	denotes	predicted
T15569	33696-33701	NN	denotes	NF-κB
T15570	33702-33709	NN	denotes	binding
T15571	33710-33715	NN	denotes	sites
T15572	33716-33718	IN	denotes	of
T15573	33719-33722	DT	denotes	the
T15574	33723-33729	NN	denotes	MIP-1α
T15575	33730-33738	NN	denotes	promoter
T15576	33738-33739	-COMMA-	denotes	,
T15577	33740-33742	PRP	denotes	we
T15578	33743-33753	VB	denotes	stimulated
T15579	33754-33758	NN	denotes	HeLa
T15580	33759-33764	NN	denotes	cells
T15581	33765-33769	IN	denotes	with
T15582	33770-33775	NN	denotes	TNF-α
T15583	33775-33776	-COMMA-	denotes	,
T15584	33777-33778	DT	denotes	a
T15585	33779-33789	JJ	denotes	well-known
T15586	33790-33795	NN	denotes	NF-κB
T15587	33796-33803	NN	denotes	inducer
T15588	33803-33804	-COMMA-	denotes	,
T15589	33805-33808	CC	denotes	and
T15590	33809-33817	VB	denotes	measured
T15591	33818-33821	DT	denotes	the
T15592	33822-33832	NN	denotes	expression
T15593	33833-33839	NN	denotes	levels
T15594	33840-33842	IN	denotes	of
T15595	33843-33849	NN	denotes	MIP-1α
T15596	33850-33852	IN	denotes	by
T15597	33853-33862	JJ	denotes	real-time
T15598	33863-33866	NN	denotes	PCR
T15599	33866-33867	-COMMA-	denotes	,
T15600	33868-33871	CC	denotes	and
T15601	33872-33875	DT	denotes	the
T15602	33876-33879	NN	denotes	p65
T15603	33880-33889	NN	denotes	occupancy
T15604	33890-33892	IN	denotes	of
T15605	33893-33896	DT	denotes	the
T15606	33897-33902	NN	denotes	NF-κB
T15607	33903-33908	NN	denotes	sites
T15608	33909-33911	IN	denotes	by
T15609	33912-33916	NN	denotes	ChIP
T15610	33918-33923	NN	denotes	TNF-α
T15611	33924-33933	VB	denotes	activated
T15612	33934-33937	DT	denotes	the
T15613	33938-33948	NN	denotes	expression
T15614	33949-33951	IN	denotes	of
T15615	33952-33958	NN	denotes	MIP-1α
T15616	33959-33962	CC	denotes	and
T15617	33963-33970	VB	denotes	induced
T15618	33971-33974	DT	denotes	the
T15619	33975-33978	NN	denotes	p65
T15620	33979-33990	NN	denotes	recruitment
T15621	33991-33993	TO	denotes	to
T15622	33994-33997	DT	denotes	the
T15623	33998-34004	NN	denotes	NF-κB1
T15624	34005-34009	NN	denotes	site
T15625	34010-34012	IN	denotes	of
T15626	34013-34019	NN	denotes	MIP-1α
T15627	34020-34027	IN	denotes	without
T15628	34028-34037	VB	denotes	affecting
T15629	34038-34041	DT	denotes	the
T15630	34042-34051	NN	denotes	occupancy
T15631	34052-34054	IN	denotes	of
T15632	34055-34058	DT	denotes	the
T15633	34059-34067	JJ	denotes	putative
T15634	34068-34074	NN	denotes	NF-κB2
T15635	34075-34078	CC	denotes	and
T15636	34079-34085	NN	denotes	NF-κB3
T15637	34086-34091	NN	denotes	sites
T15638	34092-34093	-LRB-	denotes	(
T15639	34093-34106	JJ	denotes	Supplementary
T15640	34107-34113	NNP	denotes	Figure
T15641	34114-34117	NN	denotes	S3B
T15642	34118-34121	CC	denotes	and
T15643	34122-34125	NN	denotes	S3C
T15644	34125-34126	-RRB-	denotes	)
T15645	34128-34131	NN	denotes	Tat
T15646	34132-34136	RB	denotes	also
T15647	34137-34145	VB	denotes	promoted
T15648	34146-34149	DT	denotes	the
T15649	34150-34161	NN	denotes	recruitment
T15650	34162-34164	IN	denotes	of
T15651	34165-34168	NN	denotes	p65
T15652	34169-34171	TO	denotes	to
T15653	34172-34175	DT	denotes	the
T15654	34176-34182	NN	denotes	NF-κB1
T15655	34183-34187	NN	denotes	site
T15656	34188-34190	IN	denotes	of
T15657	34191-34197	NN	denotes	MIP-1α
T15658	34197-34198	-COMMA-	denotes	,
T15659	34199-34204	IN	denotes	while
T15660	34205-34207	PRP	denotes	it
T15661	34208-34211	VB	denotes	did
T15662	34212-34215	RB	denotes	not
T15663	34216-34222	VB	denotes	affect
T15664	34223-34226	DT	denotes	the
T15665	34227-34235	JJ	denotes	putative
T15666	34236-34242	NN	denotes	NF-κB2
T15667	34243-34246	CC	denotes	and
T15668	34247-34253	NN	denotes	NF-κB3
T15669	34254-34259	NN	denotes	sites
T15670	34260-34261	-LRB-	denotes	(
T15671	34261-34274	JJ	denotes	Supplementary
T15672	34275-34281	NNP	denotes	Figure
T15673	34282-34285	NN	denotes	S3D
T15674	34285-34286	-RRB-	denotes	)
T15675	34288-34298	RB	denotes	Altogether
T15676	34299-34304	DT	denotes	these
T15677	34305-34312	NN	denotes	results
T15678	34313-34322	VB	denotes	indicated
T15679	34323-34327	IN	denotes	that
T15680	34328-34331	DT	denotes	the
T15681	34332-34338	NN	denotes	NF-κB1
T15682	34339-34343	NN	denotes	site
T15683	34344-34347	VB	denotes	was
T15684	34348-34351	DT	denotes	the
T15685	34352-34356	RB	denotes	only
T15686	34357-34366	JJ	denotes	effective
T15687	34367-34372	NN	denotes	NF-κB
T15688	34373-34381	NN	denotes	enhancer
T15689	34382-34384	IN	denotes	of
T15690	34385-34388	DT	denotes	the
T15691	34389-34395	NN	denotes	MIP-1α
T15692	34396-34404	NN	denotes	promoter
T15693	34405-34407	IN	denotes	in
T15694	34408-34416	NN	denotes	response
T15695	34417-34419	TO	denotes	to
T15696	34420-34425	NN	denotes	TNF-α
T15697	34426-34429	CC	denotes	and
T15698	34430-34433	NN	denotes	Tat
T15699	34435-34444	RB	denotes	Similarly
T15700	34445-34447	TO	denotes	to
T15701	34448-34454	NN	denotes	MIP-1α
T15702	34454-34455	-COMMA-	denotes	,
T15703	34456-34459	NN	denotes	Tat
T15704	34460-34469	VB	denotes	increased
T15705	34470-34473	DT	denotes	the
T15706	34474-34485	NN	denotes	recruitment
T15707	34486-34488	IN	denotes	of
T15708	34489-34492	NN	denotes	p65
T15709	34493-34495	TO	denotes	to
T15710	34496-34499	DT	denotes	the
T15711	34500-34505	NN	denotes	NF-κB
T15712	34506-34515	NN	denotes	enhancers
T15713	34516-34518	IN	denotes	of
T15714	34519-34523	NN	denotes	CSF3
T15715	34523-34524	-COMMA-	denotes	,
T15716	34525-34528	NN	denotes	LTA
T15717	34528-34529	-COMMA-	denotes	,
T15718	34530-34536	NN	denotes	NFKBIA
T15719	34537-34540	CC	denotes	and
T15720	34541-34545	NN	denotes	TLR2
T15721	34545-34546	-COMMA-	denotes	,
T15722	34547-34552	IN	denotes	while
T15723	34553-34555	PRP	denotes	it
T15724	34556-34559	VB	denotes	was
T15725	34560-34571	JJ	denotes	ineffective
T15726	34572-34574	IN	denotes	at
T15727	34575-34578	DT	denotes	the
T15728	34579-34588	NN	denotes	promoters
T15729	34589-34591	IN	denotes	of
T15730	34592-34597	NN	denotes	GAPDH
T15731	34598-34601	CC	denotes	and
T15732	34602-34606	NN	denotes	ACTB
T15733	34607-34608	-LRB-	denotes	(
T15734	34608-34614	NN	denotes	Figure
T15735	34615-34617	NN	denotes	5H
T15736	34617-34618	-RRB-	denotes	)
T15737	34620-34622	IN	denotes	By
T15738	34623-34627	NN	denotes	ChIP
T15739	34627-34628	-COMMA-	denotes	,
T15740	34629-34632	NN	denotes	Tat
T15741	34633-34638	VB	denotes	bound
T15742	34639-34641	TO	denotes	to
T15743	34642-34645	DT	denotes	the
T15744	34646-34662	JJ	denotes	NF-κB-responsive
T15745	34663-34672	NN	denotes	promoters
T15746	34672-34673	-COMMA-	denotes	,
T15747	34674-34677	CC	denotes	and
T15748	34678-34681	RB	denotes	not
T15749	34682-34684	TO	denotes	to
T15750	34685-34688	DT	denotes	the
T15751	34689-34694	NN	denotes	GAPDH
T15752	34695-34698	CC	denotes	and
T15753	34699-34703	NN	denotes	ACTB
T15754	34704-34713	NN	denotes	promoters
T15755	34713-34714	-COMMA-	denotes	,
T15756	34715-34719	IN	denotes	with
T15757	34720-34724	NN	denotes	loss
T15758	34725-34727	IN	denotes	of
T15759	34728-34735	NN	denotes	binding
T15760	34736-34745	VB	denotes	following
T15761	34746-34749	NN	denotes	p65
T15762	34750-34753	NN	denotes	RNA
T15763	34754-34766	NN	denotes	interference
T15764	34767-34768	-LRB-	denotes	(
T15765	34768-34774	NN	denotes	Figure
T15766	34775-34777	NN	denotes	5I
T15767	34777-34778	-RRB-	denotes	)
T15768	34778-34779	-COMMA-	denotes	,
T15769	34780-34790	VB	denotes	suggesting
T15770	34791-34795	IN	denotes	that
T15771	34796-34799	NN	denotes	Tat
T15772	34800-34809	NN	denotes	occupancy
T15773	34810-34818	VB	denotes	occurred
T15774	34819-34822	IN	denotes	via
T15775	34823-34826	NN	denotes	p65
T15776	34827-34838	NN	denotes	interaction
T15777	34840-34842	IN	denotes	As
T15778	34843-34853	JJ	denotes	additional
T15779	34854-34862	NN	denotes	findings
T15780	34862-34863	-COMMA-	denotes	,
T15781	34864-34869	NN	denotes	IκB-α
T15782	34870-34873	VB	denotes	was
T15783	34874-34902	JJ	denotes	chromatin-immunoprecipitated
T15784	34903-34905	IN	denotes	at
T15785	34906-34909	DT	denotes	the
T15786	34910-34915	NN	denotes	NF-κB
T15787	34916-34925	NN	denotes	enhancers
T15788	34926-34928	IN	denotes	in
T15789	34929-34936	NN	denotes	absence
T15790	34937-34939	IN	denotes	of
T15791	34940-34943	NN	denotes	Tat
T15792	34943-34944	-COMMA-	denotes	,
T15793	34945-34948	CC	denotes	and
T15794	34949-34951	PRP	denotes	it
T15795	34952-34955	VB	denotes	was
T15796	34956-34969	RB	denotes	significantly
T15797	34970-34977	VB	denotes	removed
T15798	34978-34980	IN	denotes	in
T15799	34981-34989	NN	denotes	presence
T15800	34990-34992	IN	denotes	of
T15801	34993-34996	NN	denotes	Tat
T15802	34996-34997	-COMMA-	denotes	,
T15803	34998-35000	CC	denotes	or
T15804	35001-35010	VB	denotes	following
T15805	35011-35016	JJ	denotes	IκB-α
T15806	35017-35020	NN	denotes	RNA
T15807	35021-35033	NN	denotes	interference
T15808	35034-35035	-LRB-	denotes	(
T15809	35035-35041	NN	denotes	Figure
T15810	35042-35044	NN	denotes	5J
T15811	35044-35045	-RRB-	denotes	)
T15812	35045-35046	-COMMA-	denotes	,
T15813	35047-35057	VB	denotes	indicating
T15814	35058-35062	IN	denotes	that
T15815	35063-35066	NN	denotes	Tat
T15816	35067-35076	VB	denotes	displaced
T15817	35077-35082	JJ	denotes	IκB-α
T15818	35083-35087	IN	denotes	from
T15819	35088-35097	NN	denotes	promoters
T17795	35100-35102	IN	denotes	In
T17796	35103-35117	JJ	denotes	HIV-1-infected
T17797	35118-35127	NN	denotes	monocytes
T17798	35128-35131	NN	denotes	Tat
T17799	35132-35140	VB	denotes	sustains
T17800	35141-35144	DT	denotes	the
T17801	35145-35150	NN	denotes	NF-κB
T17802	35151-35159	NN	denotes	activity
T17803	35160-35163	CC	denotes	and
T17804	35164-35172	VB	denotes	promotes
T17805	35173-35176	DT	denotes	the
T17806	35177-35192	JJ	denotes	transcriptional
T17807	35193-35203	NN	denotes	activation
T17808	35204-35206	IN	denotes	of
T17809	35207-35213	NN	denotes	MIP-1α
T17810	35214-35216	IN	denotes	by
T17811	35217-35228	VB	denotes	interacting
T17812	35229-35233	IN	denotes	with
T17813	35234-35239	NN	denotes	IκB-α
T17814	35240-35243	CC	denotes	and
T17815	35244-35247	NN	denotes	p65
T17816	35248-35250	PRP	denotes	We
T17817	35251-35255	RB	denotes	next
T17818	35256-35264	VB	denotes	analysed
T17819	35265-35272	IN	denotes	whether
T17820	35273-35276	NN	denotes	Tat
T17821	35277-35285	VB	denotes	affected
T17822	35286-35289	DT	denotes	the
T17823	35290-35295	NN	denotes	NF-κB
T17824	35296-35304	NN	denotes	activity
T17825	35305-35308	CC	denotes	and
T17826	35309-35312	DT	denotes	the
T17827	35313-35323	NN	denotes	expression
T17828	35324-35326	IN	denotes	of
T17829	35327-35333	NN	denotes	MIP-1α
T17830	35334-35336	IN	denotes	in
T17831	35337-35340	DT	denotes	the
T17832	35341-35347	NN	denotes	course
T17833	35348-35350	IN	denotes	of
T17834	35351-35356	NN	denotes	HIV-1
T17835	35357-35366	NN	denotes	infection
T17836	35368-35370	TO	denotes	To
T17837	35371-35377	VB	denotes	detect
T17838	35378-35381	DT	denotes	the
T17839	35382-35385	NN	denotes	Tat
T17840	35386-35393	NN	denotes	protein
T17841	35393-35394	-COMMA-	denotes	,
T17842	35395-35397	PRP	denotes	we
T17843	35398-35406	VB	denotes	produced
T17844	35407-35411	NN	denotes	HXB2
T17845	35412-35427	JJ	denotes	Env-pseudotyped
T17846	35428-35447	NN	denotes	NL4-3.FLAG-Tat.R−E−
T17847	35448-35455	NN	denotes	virions
T17848	35455-35456	-COMMA-	denotes	,
T17849	35457-35462	WDT	denotes	which
T17850	35463-35467	VB	denotes	were
T17851	35468-35472	VB	denotes	used
T17852	35473-35475	IN	denotes	in
T17853	35476-35488	JJ	denotes	single-round
T17854	35489-35498	NN	denotes	infection
T17855	35499-35501	IN	denotes	of
T17856	35502-35506	NN	denotes	U937
T17857	35506-35507	-COMMA-	denotes	,
T17858	35508-35509	DT	denotes	a
T17859	35510-35515	JJ	denotes	human
T17860	35516-35525	JJ	denotes	monocytic
T17861	35526-35530	NN	denotes	cell
T17862	35531-35535	NN	denotes	line
T17863	35537-35542	NN	denotes	Cells
T17864	35543-35547	VB	denotes	were
T17865	35548-35559	VB	denotes	transfected
T17866	35560-35564	IN	denotes	with
T17867	35565-35570	NN	denotes	siRNA
T17868	35571-35574	NN	denotes	Tat
T17869	35574-35575	-COMMA-	denotes	,
T17870	35576-35581	NN	denotes	siRNA
T17871	35582-35589	NN	denotes	control
T17872	35590-35592	CC	denotes	or
T17873	35593-35597	VB	denotes	left
T17874	35598-35611	JJ	denotes	untransfected
T17875	35612-35614	TO	denotes	to
T17876	35615-35623	VB	denotes	modulate
T17877	35624-35627	DT	denotes	the
T17878	35628-35631	NN	denotes	Tat
T17879	35632-35642	NN	denotes	expression
T17880	35642-35643	-COMMA-	denotes	,
T17881	35644-35647	CC	denotes	and
T17882	35648-35652	RB	denotes	then
T17883	35653-35661	VB	denotes	analysed
T17884	35662-35665	IN	denotes	for
T17885	35666-35669	DT	denotes	the
T17886	35670-35677	NN	denotes	kinetic
T17887	35678-35680	IN	denotes	of
T17888	35681-35686	NN	denotes	NF-κB
T17889	35687-35697	NN	denotes	activation
T17890	35698-35707	VB	denotes	following
T17891	35708-35713	JJ	denotes	viral
T17892	35714-35723	NN	denotes	infection
T17893	35725-35728	DT	denotes	The
T17894	35729-35739	NN	denotes	expression
T17895	35740-35742	IN	denotes	of
T17896	35743-35746	NN	denotes	Tat
T17897	35747-35750	CC	denotes	and
T17898	35751-35754	NN	denotes	p24
T17899	35755-35758	VB	denotes	was
T17900	35759-35767	VB	denotes	detected
T17901	35768-35770	IN	denotes	at
T17902	35771-35773	CD	denotes	3-
T17903	35774-35776	TO	denotes	to
T17904	35777-35781	JJ	denotes	12-h
T17905	35782-35796	NN	denotes	post-infection
T17906	35796-35797	-COMMA-	denotes	,
T17907	35798-35803	IN	denotes	while
T17908	35804-35806	PRP	denotes	it
T17909	35807-35810	VB	denotes	was
T17910	35811-35817	RB	denotes	barely
T17911	35818-35826	VB	denotes	detected
T17912	35827-35829	IN	denotes	in
T17913	35830-35835	NN	denotes	siRNA
T17914	35836-35851	JJ	denotes	Tat-transfected
T17915	35852-35857	NN	denotes	cells
T17916	35858-35859	-LRB-	denotes	(
T17917	35859-35865	NN	denotes	Figure
T17918	35866-35868	NN	denotes	6A
T17919	35868-35869	-RRB-	denotes	)
T17920	38126-38129	DT	denotes	The
T17921	38130-38135	NN	denotes	NF-κB
T17922	38136-38144	NN	denotes	activity
T17923	38144-38145	-COMMA-	denotes	,
T17924	38146-38148	IN	denotes	as
T17925	38149-38157	VB	denotes	measured
T17926	38158-38160	IN	denotes	by
T17927	38161-38164	NN	denotes	p65
T17928	38165-38168	NN	denotes	DNA
T17929	38169-38176	NN	denotes	binding
T17930	38176-38177	-COMMA-	denotes	,
T17931	38178-38181	VB	denotes	was
T17932	38182-38189	VB	denotes	induced
T17933	38190-38192	IN	denotes	at
T17934	38193-38196	JJ	denotes	3-h
T17935	38197-38211	NN	denotes	post-infection
T17936	38212-38214	IN	denotes	in
T17937	38215-38219	CC	denotes	both
T17938	38220-38232	JJ	denotes	Tat-positive
T17939	38233-38234	-LRB-	denotes	(
T17940	38234-38236	DT	denotes	no
T17941	38237-38242	NN	denotes	siRNA
T17942	38243-38246	CC	denotes	and
T17943	38247-38252	NN	denotes	siRNA
T17944	38253-38260	NN	denotes	control
T17945	38260-38261	-RRB-	denotes	)
T17946	38262-38265	CC	denotes	and
T17947	38266-38278	JJ	denotes	Tat-negative
T17948	38279-38284	NN	denotes	cells
T17949	38285-38286	-LRB-	denotes	(
T17950	38286-38291	NN	denotes	siRNA
T17951	38292-38295	NN	denotes	Tat
T17952	38295-38296	-RRB-	denotes	)
T17953	38296-38297	-COMMA-	denotes	,
T17954	38298-38301	CC	denotes	and
T17955	38302-38311	VB	denotes	increased
T17956	38312-38314	IN	denotes	at
T17957	38315-38319	NN	denotes	12 h
T17958	38320-38324	RB	denotes	only
T17959	38325-38327	IN	denotes	in
T17960	38328-38340	JJ	denotes	Tat-positive
T17961	38341-38346	NN	denotes	cells
T17962	38347-38348	-LRB-	denotes	(
T17963	38348-38354	NN	denotes	Figure
T17964	38355-38357	NN	denotes	6B
T17965	38357-38358	-RRB-	denotes	)
T17966	38360-38371	NN	denotes	Degradation
T17967	38372-38374	IN	denotes	of
T17968	38375-38380	NN	denotes	IκB-α
T17969	38381-38384	VB	denotes	was
T17970	38385-38393	VB	denotes	observed
T17971	38394-38396	IN	denotes	at
T17972	38397-38400	JJ	denotes	3-h
T17973	38401-38415	NN	denotes	post-infection
T17974	38415-38416	-COMMA-	denotes	,
T17975	38417-38420	CC	denotes	and
T17976	38421-38424	VB	denotes	was
T17977	38425-38433	VB	denotes	followed
T17978	38434-38436	IN	denotes	by
T17979	38437-38439	FW	denotes	de
T17980	38440-38444	FW	denotes	novo
T17981	38445-38454	NN	denotes	synthesis
T17982	38455-38457	IN	denotes	of
T17983	38458-38463	NN	denotes	IκB-α
T17984	38464-38466	IN	denotes	at
T17985	38467-38471	CD	denotes	12 h
T17986	38472-38485	RB	denotes	independently
T17987	38486-38488	IN	denotes	of
T17988	38489-38492	DT	denotes	the
T17989	38493-38496	NN	denotes	Tat
T17990	38497-38505	NN	denotes	presence
T17991	38506-38507	-LRB-	denotes	(
T17992	38507-38513	NN	denotes	Figure
T17993	38514-38516	NN	denotes	6C
T17994	38516-38517	-RRB-	denotes	)
T17995	38519-38531	RB	denotes	Consistently
T17996	38531-38532	-COMMA-	denotes	,
T17997	38533-38536	DT	denotes	the
T17998	38537-38540	NN	denotes	IKK
T17999	38541-38549	NN	denotes	activity
T18000	38550-38553	VB	denotes	was
T18001	38554-38561	VB	denotes	induced
T18002	38562-38564	IN	denotes	at
T18003	38565-38568	JJ	denotes	3-h
T18004	38569-38583	NN	denotes	post-infection
T18005	38584-38587	CC	denotes	and
T18006	38588-38594	VB	denotes	turned
T18007	38595-38598	RP	denotes	off
T18008	38599-38601	IN	denotes	at
T18009	38602-38606	CD	denotes	12 h
T18010	38607-38620	RB	denotes	independently
T18011	38621-38623	IN	denotes	of
T18012	38624-38627	DT	denotes	the
T18013	38628-38631	NN	denotes	Tat
T18014	38632-38640	NN	denotes	presence
T18015	38641-38642	-LRB-	denotes	(
T18016	38642-38648	NN	denotes	Figure
T18017	38649-38651	NN	denotes	6D
T18018	38651-38652	-RRB-	denotes	)
T18019	38654-38659	DT	denotes	These
T18020	38660-38667	NN	denotes	results
T18021	38668-38674	VB	denotes	agreed
T18022	38675-38679	IN	denotes	with
T18023	38680-38683	DT	denotes	the
T18024	38684-38691	NN	denotes	kinetic
T18025	38692-38694	IN	denotes	of
T18026	38695-38700	NN	denotes	NF-κB
T18027	38701-38711	NN	denotes	activation
T18028	38712-38714	IN	denotes	in
T18029	38715-38727	JJ	denotes	single-round
T18030	38728-38733	NN	denotes	HIV-1
T18031	38734-38743	NN	denotes	infection
T18032	38744-38746	IN	denotes	of
T18033	38747-38753	NN	denotes	Jurkat
T18034	38754-38759	NN	denotes	cells
T18035	38760-38761	-LRB-	denotes	(
T18036	38761-38767	NN	denotes	Figure
T18037	38768-38769	CD	denotes	1
T18038	38769-38770	-RRB-	denotes	)
T18039	38770-38771	-COMMA-	denotes	,
T18040	38772-38775	CC	denotes	and
T18041	38776-38783	RB	denotes	further
T18042	38784-38793	VB	denotes	supported
T18043	38794-38797	DT	denotes	the
T18044	38798-38809	NN	denotes	requirement
T18045	38810-38812	IN	denotes	of
T18046	38813-38818	JJ	denotes	viral
T18047	38819-38829	NN	denotes	expression
T18048	38830-38832	TO	denotes	to
T18049	38833-38843	VB	denotes	counteract
T18050	38844-38847	DT	denotes	the
T18051	38848-38863	JJ	denotes	post-activation
T18052	38864-38868	NN	denotes	turn
T18053	38869-38872	RP	denotes	off
T18054	38873-38875	IN	denotes	of
T18055	38876-38881	NN	denotes	NF-κB
T18056	38883-38885	IN	denotes	In
T18057	38886-38900	JJ	denotes	HIV-1-infected
T18058	38901-38905	NN	denotes	U937
T18059	38906-38911	NN	denotes	cells
T18060	38911-38912	-COMMA-	denotes	,
T18061	38913-38916	DT	denotes	the
T18062	38917-38927	JJ	denotes	endogenous
T18063	38928-38931	NN	denotes	Tat
T18064	38932-38935	VB	denotes	was
T18065	38936-38956	VB	denotes	coimmunoprecipitated
T18066	38957-38961	IN	denotes	with
T18067	38962-38967	NN	denotes	IκB-α
T18068	38968-38971	CC	denotes	and
T18069	38972-38975	NN	denotes	p65
T18070	38976-38977	-LRB-	denotes	(
T18071	38977-38983	NN	denotes	Figure
T18072	38984-38986	NN	denotes	6E
T18073	38986-38987	-RRB-	denotes	)
T18074	38987-38988	-COMMA-	denotes	,
T18075	38989-38992	CC	denotes	and
T18076	38993-39002	VB	denotes	activated
T18077	39003-39006	DT	denotes	the
T18078	39007-39013	NN	denotes	MIP-1α
T18079	39014-39024	NN	denotes	expression
T18080	39025-39027	IN	denotes	in
T18081	39028-39029	DT	denotes	a
T18082	39030-39043	JJ	denotes	p65-dependent
T18083	39044-39050	NN	denotes	manner
T18084	39050-39051	-COMMA-	denotes	,
T18085	39052-39054	IN	denotes	as
T18086	39055-39060	VB	denotes	shown
T18087	39061-39063	IN	denotes	by
T18088	39064-39067	DT	denotes	the
T18089	39068-39072	NN	denotes	lack
T18090	39073-39075	IN	denotes	of
T18091	39076-39082	NN	denotes	effect
T18092	39083-39092	VB	denotes	following
T18093	39093-39096	NN	denotes	RNA
T18094	39097-39109	NN	denotes	interference
T18095	39110-39112	IN	denotes	of
T18096	39113-39116	NN	denotes	Tat
T18097	39117-39119	CC	denotes	or
T18098	39120-39123	NN	denotes	p65
T18099	39124-39125	-LRB-	denotes	(
T18100	39125-39131	NN	denotes	Figure
T18101	39132-39134	NN	denotes	6F
T18102	39134-39135	-RRB-	denotes	)
T18103	39137-39145	RB	denotes	Moreover
T18104	39145-39146	-COMMA-	denotes	,
T18105	39147-39150	NN	denotes	Tat
T18106	39151-39154	CC	denotes	and
T18107	39155-39158	NN	denotes	p65
T18108	39159-39163	VB	denotes	were
T18109	39164-39168	DT	denotes	both
T18110	39169-39178	VB	denotes	recruited
T18111	39179-39181	TO	denotes	to
T18112	39182-39185	DT	denotes	the
T18113	39186-39191	NN	denotes	NF-κB
T18114	39192-39200	NN	denotes	enhancer
T18115	39201-39203	IN	denotes	of
T18116	39204-39210	NN	denotes	MIP-1α
T18117	39210-39211	-COMMA-	denotes	,
T18118	39212-39217	IN	denotes	while
T18119	39218-39221	DT	denotes	the
T18120	39222-39225	NN	denotes	p65
T18121	39226-39235	NN	denotes	occupancy
T18122	39236-39239	VB	denotes	was
T18123	39240-39249	VB	denotes	abolished
T18124	39250-39252	IN	denotes	by
T18125	39253-39258	NN	denotes	siRNA
T18126	39259-39262	NN	denotes	Tat
T18127	39263-39264	-LRB-	denotes	(
T18128	39264-39270	NN	denotes	Figure
T18129	39271-39273	NN	denotes	6G
T18130	39274-39277	CC	denotes	and
T18131	39278-39279	NN	denotes	H
T18132	39279-39280	-RRB-	denotes	)
T18133	39282-39292	RB	denotes	Altogether
T18134	39293-39298	DT	denotes	these
T18135	39299-39306	NN	denotes	results
T18136	39307-39316	VB	denotes	indicated
T18137	39317-39321	IN	denotes	that
T18138	39322-39324	IN	denotes	in
T18139	39325-39331	JJ	denotes	single
T18140	39332-39337	JJ	denotes	round
T18141	39338-39343	NN	denotes	HIV-1
T18142	39344-39353	NN	denotes	infection
T18143	39354-39357	NN	denotes	Tat
T18144	39358-39368	VB	denotes	associated
T18145	39369-39373	IN	denotes	with
T18146	39374-39379	NN	denotes	IκB-α
T18147	39380-39383	CC	denotes	and
T18148	39384-39387	NN	denotes	p65
T18149	39387-39388	-COMMA-	denotes	,
T18150	39389-39392	CC	denotes	and
T18151	39393-39400	VB	denotes	induced
T18152	39401-39404	DT	denotes	the
T18153	39405-39418	JJ	denotes	p65-dependent
T18154	39419-39429	NN	denotes	activation
T18155	39430-39432	IN	denotes	of
T18156	39433-39439	NN	denotes	MIP-1α
T18157	39440-39450	NN	denotes	expression
T18158	39451-39458	IN	denotes	through
T18159	39459-39468	NN	denotes	occupancy
T18160	39469-39471	IN	denotes	of
T18161	39472-39475	DT	denotes	the
T18162	39476-39482	NN	denotes	MIP-1α
T18163	39483-39491	NN	denotes	promoter
T18164	39492-39495	CC	denotes	and
T18165	39496-39505	VB	denotes	increased
T18166	39506-39517	NN	denotes	recruitment
T18167	39518-39520	IN	denotes	of
T18168	39521-39524	NN	denotes	p65
T19911	39538-39542	DT	denotes	This
T19912	39543-39548	NN	denotes	study
T19913	39549-39556	VB	denotes	reports
T19914	39557-39558	DT	denotes	a
T19915	39559-39564	JJ	denotes	novel
T19916	39565-39574	NN	denotes	mechanism
T19917	39575-39577	IN	denotes	of
T19918	39578-39583	NN	denotes	NF-κB
T19919	39584-39594	NN	denotes	activation
T19920	39595-39597	IN	denotes	by
T19921	39598-39601	DT	denotes	the
T19922	39602-39607	NN	denotes	HIV-1
T19923	39608-39611	NN	denotes	Tat
T19924	39612-39626	NN	denotes	transactivator
T19925	39628-39633	VB	denotes	Based
T19926	39634-39636	IN	denotes	on
T19927	39637-39640	DT	denotes	the
T19928	39641-39649	NN	denotes	evidence
T19929	39650-39654	IN	denotes	that
T19930	39655-39658	NN	denotes	Tat
T19931	39659-39667	VB	denotes	enhanced
T19932	39668-39671	DT	denotes	the
T19933	39672-39687	JJ	denotes	transcriptional
T19934	39688-39696	NN	denotes	activity
T19935	39697-39699	IN	denotes	of
T19936	39700-39703	DT	denotes	the
T19937	39704-39707	NN	denotes	p65
T19938	39708-39715	NN	denotes	subunit
T19939	39716-39718	IN	denotes	of
T19940	39719-39724	NN	denotes	NF-κB
T19941	39725-39726	-LRB-	denotes	(
T19942	39726-39734	CD	denotes	49,63,64
T19943	39734-39735	-RRB-	denotes	)
T19944	39735-39736	-COMMA-	denotes	,
T19945	39737-39740	CC	denotes	and
T19946	39741-39751	RB	denotes	physically
T19947	39752-39762	VB	denotes	interacted
T19948	39763-39767	IN	denotes	with
T19949	39768-39771	DT	denotes	the
T19950	39772-39777	JJ	denotes	IκB-α
T19951	39778-39787	NN	denotes	repressor
T19952	39788-39789	-LRB-	denotes	(
T19953	39789-39794	CD	denotes	50,51
T19954	39794-39795	-RRB-	denotes	)
T19955	39795-39796	-COMMA-	denotes	,
T19956	39797-39799	PRP	denotes	we
T19957	39800-39812	VB	denotes	investigated
T19958	39813-39816	DT	denotes	the
T19959	39817-39828	NN	denotes	possibility
T19960	39829-39833	IN	denotes	that
T19961	39834-39837	NN	denotes	Tat
T19962	39838-39843	MD	denotes	could
T19963	39844-39852	VB	denotes	activate
T19964	39853-39858	NN	denotes	NF-κB
T19965	39859-39862	IN	denotes	via
T19966	39863-39869	JJ	denotes	direct
T19967	39870-39881	NN	denotes	interaction
T19968	39882-39886	IN	denotes	with
T19969	39887-39892	NN	denotes	IκB-α
T19970	39893-39896	CC	denotes	and
T19971	39897-39900	NN	denotes	p65
T19972	39902-39904	TO	denotes	To
T19973	39905-39909	DT	denotes	this
T19974	39910-39913	NN	denotes	end
T19975	39913-39914	-COMMA-	denotes	,
T19976	39915-39918	DT	denotes	the
T19977	39919-39924	NN	denotes	NF-κB
T19978	39925-39933	NN	denotes	activity
T19979	39934-39937	VB	denotes	was
T19980	39938-39947	VB	denotes	monitored
T19981	39948-39950	IN	denotes	in
T19982	39951-39957	JJ	denotes	single
T19983	39958-39963	JJ	denotes	round
T19984	39964-39969	NN	denotes	HIV-1
T19985	39970-39979	NN	denotes	infection
T19986	39980-39985	VB	denotes	using
T19987	39986-39989	NN	denotes	RNA
T19988	39990-40002	NN	denotes	interference
T19989	40003-40005	TO	denotes	to
T19990	40006-40013	VB	denotes	silence
T19991	40014-40017	DT	denotes	the
T19992	40018-40021	NN	denotes	Tat
T19993	40022-40032	NN	denotes	expression
T19994	40034-40036	IN	denotes	By
T19995	40037-40041	DT	denotes	this
T19996	40042-40050	NN	denotes	approach
T19997	40050-40051	-COMMA-	denotes	,
T19998	40052-40054	PRP	denotes	we
T19999	40055-40062	VB	denotes	avoided
T20000	40063-40066	DT	denotes	the
T20001	40067-40079	NN	denotes	perpetuation
T20002	40080-40082	IN	denotes	of
T20003	40083-40088	NN	denotes	NF-κB
T20004	40089-40099	NN	denotes	activation
T20005	40100-40109	NN	denotes	signaling
T20006	40110-40113	JJ	denotes	due
T20007	40114-40116	TO	denotes	to
T20008	40117-40127	JJ	denotes	subsequent
T20009	40128-40134	NN	denotes	rounds
T20010	40135-40137	IN	denotes	of
T20011	40138-40143	JJ	denotes	viral
T20012	40144-40149	NN	denotes	entry
T20013	40150-40152	IN	denotes	in
T20014	40153-40157	NN	denotes	cell
T20015	40158-40165	NN	denotes	culture
T20016	40166-40177	NN	denotes	propagation
T20017	40178-40179	-LRB-	denotes	(
T20018	40179-40184	CD	denotes	30,31
T20019	40184-40185	-RRB-	denotes	)
T20020	40187-40191	IN	denotes	Upon
T20021	40192-40197	NN	denotes	HIV-1
T20022	40198-40207	NN	denotes	infection
T20023	40207-40208	-COMMA-	denotes	,
T20024	40209-40212	DT	denotes	the
T20025	40213-40218	JJ	denotes	early
T20026	40219-40224	NN	denotes	NF-κB
T20027	40225-40235	NN	denotes	activation
T20028	40236-40244	VB	denotes	occurred
T20029	40245-40258	RB	denotes	concomitantly
T20030	40259-40263	IN	denotes	with
T20031	40264-40267	NN	denotes	IKK
T20032	40268-40278	NN	denotes	activation
T20033	40279-40282	CC	denotes	and
T20034	40283-40288	JJ	denotes	IκB-α
T20035	40289-40300	NN	denotes	degradation
T20036	40301-40303	IN	denotes	in
T20037	40304-40307	DT	denotes	the
T20038	40308-40315	NN	denotes	absence
T20039	40316-40318	IN	denotes	of
T20040	40319-40323	NNP	denotes	Tat.
T20041	40324-40328	NNP	denotes	Soon
T20042	40329-40334	IN	denotes	after
T20043	40335-40338	DT	denotes	the
T20044	40339-40343	NN	denotes	shut
T20045	40344-40347	RP	denotes	off
T20046	40348-40350	IN	denotes	of
T20047	40351-40354	NN	denotes	IKK
T20048	40355-40363	NN	denotes	activity
T20049	40364-40367	CC	denotes	and
T20050	40368-40371	JJ	denotes	new
T20051	40372-40381	NN	denotes	synthesis
T20052	40382-40384	IN	denotes	of
T20053	40385-40390	NN	denotes	IκB-α
T20054	40390-40391	-COMMA-	denotes	,
T20055	40392-40395	DT	denotes	the
T20056	40396-40401	NN	denotes	NF-κB
T20057	40402-40410	NN	denotes	activity
T20058	40411-40414	VB	denotes	was
T20059	40415-40419	VB	denotes	kept
T20060	40420-40428	JJ	denotes	elevated
T20061	40429-40431	IN	denotes	in
T20062	40432-40435	DT	denotes	the
T20063	40436-40444	NN	denotes	presence
T20064	40445-40447	IN	denotes	of
T20065	40448-40451	NN	denotes	Tat
T20066	40451-40452	-COMMA-	denotes	,
T20067	40453-40458	IN	denotes	while
T20068	40459-40461	PRP	denotes	it
T20069	40462-40465	VB	denotes	was
T20070	40466-40470	RB	denotes	down
T20071	40471-40480	VB	denotes	regulated
T20072	40481-40485	IN	denotes	upon
T20073	40486-40495	NN	denotes	silencing
T20074	40496-40498	IN	denotes	of
T20075	40499-40502	DT	denotes	the
T20076	40503-40506	NN	denotes	Tat
T20077	40507-40511	NN	denotes	gene
T20078	40513-40518	DT	denotes	These
T20079	40519-40527	NN	denotes	findings
T20080	40528-40536	VB	denotes	indicate
T20081	40537-40541	IN	denotes	that
T20082	40542-40544	IN	denotes	in
T20083	40545-40551	JJ	denotes	single
T20084	40552-40557	JJ	denotes	round
T20085	40558-40563	NN	denotes	HIV-1
T20086	40564-40573	NN	denotes	infection
T20087	40573-40574	-COMMA-	denotes	,
T20088	40575-40578	NN	denotes	Tat
T20089	40579-40587	VB	denotes	enhanced
T20090	40588-40591	DT	denotes	the
T20091	40592-40597	NN	denotes	NF-κB
T20092	40598-40606	NN	denotes	activity
T20093	40607-40614	IN	denotes	without
T20094	40615-40624	VB	denotes	affecting
T20095	40625-40628	DT	denotes	the
T20096	40629-40632	NN	denotes	IKK
T20097	40633-40641	NN	denotes	activity
T20098	40642-40645	CC	denotes	and
T20099	40646-40649	DT	denotes	the
T20100	40650-40659	NN	denotes	half-life
T20101	40660-40662	IN	denotes	of
T20102	40663-40668	NN	denotes	IκB-α
T20103	40670-40677	JJ	denotes	Similar
T20104	40678-40685	JJ	denotes	kinetic
T20105	40686-40688	IN	denotes	of
T20106	40689-40694	NN	denotes	NF-κB
T20107	40695-40705	NN	denotes	activation
T20108	40706-40714	VB	denotes	occurred
T20109	40715-40719	IN	denotes	upon
T20110	40720-40731	NN	denotes	short-pulse
T20111	40732-40734	IN	denotes	of
T20112	40735-40738	NN	denotes	PMA
T20113	40739-40741	IN	denotes	in
T20114	40742-40757	JJ	denotes	Tat-transfected
T20115	40758-40762	NN	denotes	HeLa
T20116	40763-40768	NN	denotes	cells
T20117	40768-40769	-COMMA-	denotes	,
T20118	40770-40775	WRB	denotes	where
T20119	40776-40779	NN	denotes	Tat
T20120	40780-40789	VB	denotes	inhibited
T20121	40790-40793	DT	denotes	the
T20122	40794-40809	JJ	denotes	post-activation
T20123	40810-40814	NN	denotes	turn
T20124	40815-40818	RP	denotes	off
T20125	40819-40821	IN	denotes	of
T20126	40822-40827	NN	denotes	NF-κB
T20127	40828-40830	IN	denotes	in
T20128	40831-40839	NN	denotes	presence
T20129	40840-40842	IN	denotes	of
T20130	40843-40848	RB	denotes	newly
T20131	40849-40860	VB	denotes	synthesized
T20132	40861-40866	NN	denotes	IκB-α
T20133	40868-40871	DT	denotes	The
T20134	40872-40885	JJ	denotes	Tat-dependent
T20135	40886-40896	NN	denotes	activation
T20136	40897-40899	IN	denotes	of
T20137	40900-40905	NN	denotes	NF-κB
T20138	40906-40916	VB	denotes	correlated
T20139	40917-40921	IN	denotes	with
T20140	40922-40925	DT	denotes	the
T20141	40926-40937	NN	denotes	association
T20142	40938-40940	IN	denotes	of
T20143	40941-40944	DT	denotes	the
T20144	40945-40950	JJ	denotes	viral
T20145	40951-40958	NN	denotes	protein
T20146	40959-40963	IN	denotes	with
T20147	40964-40969	NN	denotes	IκB-α
T20148	40969-40970	-COMMA-	denotes	,
T20149	40971-40976	WDT	denotes	which
T20150	40977-40987	VB	denotes	interfered
T20151	40988-40992	IN	denotes	with
T20152	40993-40996	DT	denotes	the
T20153	40997-41007	NN	denotes	generation
T20154	41008-41010	IN	denotes	of
T20155	41011-41014	DT	denotes	the
T20156	41015-41024	NN	denotes	IκB-α/p65
T20157	41025-41032	NN	denotes	complex
T20158	41034-41038	DT	denotes	This
T20159	41039-41047	NN	denotes	evidence
T20160	41048-41060	VB	denotes	demonstrated
T20161	41061-41065	IN	denotes	that
T20162	41066-41069	NN	denotes	Tat
T20163	41070-41078	VB	denotes	competed
T20164	41079-41082	IN	denotes	for
T20165	41083-41086	DT	denotes	the
T20166	41087-41094	NN	denotes	binding
T20167	41095-41097	IN	denotes	of
T20168	41098-41103	NN	denotes	IκB-α
T20169	41104-41106	TO	denotes	to
T20170	41107-41110	NN	denotes	p65
T20171	41111-41114	CC	denotes	and
T20172	41115-41124	VB	denotes	prevented
T20173	41125-41128	DT	denotes	the
T20174	41129-41134	JJ	denotes	IκB-α
T20175	41135-41145	NN	denotes	repression
T20176	41146-41148	IN	denotes	of
T20177	41149-41152	NN	denotes	p65
T20178	41154-41156	IN	denotes	As
T20179	41157-41167	JJ	denotes	additional
T20180	41168-41177	NN	denotes	mechanism
T20181	41178-41180	IN	denotes	of
T20182	41181-41186	NN	denotes	NF-κB
T20183	41187-41197	NN	denotes	activation
T20184	41197-41198	-COMMA-	denotes	,
T20185	41199-41202	NN	denotes	Tat
T20186	41203-41213	VB	denotes	associated
T20187	41214-41218	IN	denotes	with
T20188	41219-41222	NN	denotes	p65
T20189	41223-41226	CC	denotes	and
T20190	41227-41236	VB	denotes	increased
T20191	41237-41240	DT	denotes	the
T20192	41241-41244	NN	denotes	p65
T20193	41245-41252	NN	denotes	binding
T20194	41253-41261	NN	denotes	affinity
T20195	41262-41264	TO	denotes	to
T20196	41265-41268	DT	denotes	the
T20197	41269-41274	NN	denotes	NF-κB
T20198	41275-41283	NN	denotes	enhancer
T20199	41283-41284	-COLON-	denotes	;
T20200	41285-41289	DT	denotes	this
T20201	41290-41298	NN	denotes	evidence
T20202	41299-41302	VB	denotes	was
T20203	41303-41311	VB	denotes	obtained
T20204	41312-41316	IN	denotes	with
T20205	41317-41328	JJ	denotes	recombinant
T20206	41329-41337	NN	denotes	proteins
T20207	41338-41348	VB	denotes	indicating
T20208	41349-41353	IN	denotes	that
T20209	41354-41357	DT	denotes	the
T20210	41358-41366	JJ	denotes	stronger
T20211	41367-41374	NN	denotes	binding
T20212	41375-41377	IN	denotes	of
T20213	41378-41381	NN	denotes	p65
T20214	41382-41384	TO	denotes	to
T20215	41385-41388	NN	denotes	DNA
T20216	41389-41392	VB	denotes	was
T20217	41393-41394	DT	denotes	a
T20218	41395-41406	NN	denotes	consequence
T20219	41407-41409	IN	denotes	of
T20220	41410-41416	JJ	denotes	direct
T20221	41417-41428	NN	denotes	association
T20222	41429-41433	IN	denotes	with
T20223	41434-41437	NN	denotes	Tat
T20224	41437-41438	-COMMA-	denotes	,
T20225	41439-41444	WDT	denotes	which
T20226	41445-41451	RB	denotes	likely
T20227	41452-41458	VB	denotes	caused
T20228	41459-41460	DT	denotes	a
T20229	41461-41475	JJ	denotes	conformational
T20230	41476-41482	NN	denotes	change
T20231	41483-41485	IN	denotes	of
T20232	41486-41489	NN	denotes	p65
T20233	41491-41493	PRP	denotes	We
T20234	41494-41498	RB	denotes	also
T20235	41499-41511	VB	denotes	demonstrated
T20236	41512-41516	IN	denotes	that
T20237	41517-41520	DT	denotes	the
T20238	41521-41524	NN	denotes	Tat
T20239	41525-41538	JJ	denotes	cysteine-rich
T20240	41539-41547	NN	denotes	sequence
T20241	41548-41549	-LRB-	denotes	(
T20242	41549-41558	NN	denotes	C22,25,27
T20243	41558-41559	-RRB-	denotes	)
T20244	41560-41563	VB	denotes	was
T20245	41564-41572	VB	denotes	involved
T20246	41573-41575	IN	denotes	in
T20247	41576-41579	DT	denotes	the
T20248	41580-41587	NN	denotes	binding
T20249	41588-41590	TO	denotes	to
T20250	41591-41594	DT	denotes	the
T20251	41595-41598	NN	denotes	RHD
T20252	41599-41601	IN	denotes	of
T20253	41602-41605	NN	denotes	p65
T20254	41606-41609	CC	denotes	and
T20255	41610-41615	NN	denotes	NF-κB
T20256	41616-41626	NN	denotes	activation
T20257	41628-41639	RB	denotes	Differently
T20258	41639-41640	-COMMA-	denotes	,
T20259	41641-41644	DT	denotes	the
T20260	41645-41648	NN	denotes	Tat
T20261	41649-41662	JJ	denotes	arginine-rich
T20262	41663-41671	NN	denotes	sequence
T20263	41672-41673	-LRB-	denotes	(
T20264	41673-41691	NN	denotes	R49,52,53,55,56,57
T20265	41691-41692	-RRB-	denotes	)
T20266	41693-41696	VB	denotes	was
T20267	41697-41705	VB	denotes	required
T20268	41706-41709	IN	denotes	for
T20269	41710-41713	DT	denotes	the
T20270	41714-41721	NN	denotes	binding
T20271	41722-41724	TO	denotes	to
T20272	41725-41728	DT	denotes	the
T20273	41729-41734	JJ	denotes	sixth
T20274	41735-41742	NN	denotes	ankyrin
T20275	41743-41745	IN	denotes	of
T20276	41746-41751	NN	denotes	IκB-α
T20277	41752-41753	-LRB-	denotes	(
T20278	41753-41758	CD	denotes	50,51
T20279	41758-41759	-RRB-	denotes	)
T20280	41759-41760	-COMMA-	denotes	,
T20281	41761-41764	CC	denotes	and
T20282	41765-41768	IN	denotes	for
T20283	41769-41778	VB	denotes	releasing
T20284	41779-41782	NN	denotes	p65
T20285	41783-41787	IN	denotes	from
T20286	41788-41791	DT	denotes	the
T20287	41792-41797	JJ	denotes	IκB-α
T20288	41798-41808	NN	denotes	inhibition
T20289	41810-41820	RB	denotes	Altogether
T20290	41821-41826	DT	denotes	these
T20291	41827-41834	NN	denotes	results
T20292	41835-41847	VB	denotes	demonstrated
T20293	41848-41852	IN	denotes	that
T20294	41853-41856	NN	denotes	Tat
T20295	41857-41866	VB	denotes	abolished
T20296	41867-41870	DT	denotes	the
T20297	41871-41879	JJ	denotes	negative
T20298	41880-41888	NN	denotes	feedback
T20299	41889-41899	NN	denotes	regulation
T20300	41900-41902	IN	denotes	of
T20301	41903-41908	NN	denotes	NF-κB
T20302	41909-41911	IN	denotes	by
T20303	41912-41921	VB	denotes	hijacking
T20304	41922-41925	DT	denotes	the
T20305	41926-41931	JJ	denotes	IκB-α
T20306	41932-41941	NN	denotes	repressor
T20307	41941-41942	-COMMA-	denotes	,
T20308	41943-41952	VB	denotes	shielding
T20309	41953-41956	NN	denotes	p65
T20310	41957-41961	IN	denotes	from
T20311	41962-41965	DT	denotes	the
T20312	41966-41971	JJ	denotes	IκB-α
T20313	41972-41979	NN	denotes	embrace
T20314	41979-41980	-COMMA-	denotes	,
T20315	41981-41984	CC	denotes	and
T20316	41985-41994	VB	denotes	enforcing
T20317	41995-41998	DT	denotes	the
T20318	41999-42002	NN	denotes	p65
T20319	42003-42010	NN	denotes	binding
T20320	42011-42013	TO	denotes	to
T20321	42014-42017	NN	denotes	DNA
T20322	42019-42026	JJ	denotes	Further
T20323	42027-42035	NN	denotes	evidence
T20324	42036-42038	IN	denotes	of
T20325	42039-42042	NN	denotes	Tat
T20326	42043-42053	NN	denotes	activation
T20327	42054-42056	IN	denotes	of
T20328	42057-42062	NN	denotes	NF-κB
T20329	42063-42069	VB	denotes	showed
T20330	42070-42074	IN	denotes	that
T20331	42075-42084	JJ	denotes	wild-type
T20332	42085-42088	NN	denotes	Tat
T20333	42088-42089	-COMMA-	denotes	,
T20334	42090-42093	CC	denotes	and
T20335	42094-42097	RB	denotes	not
T20336	42098-42101	DT	denotes	the
T20337	42102-42105	NN	denotes	Tat
T20338	42106-42113	NN	denotes	mutants
T20339	42114-42121	VB	denotes	lacking
T20340	42122-42125	DT	denotes	the
T20341	42126-42135	NN	denotes	arginine-
T20342	42136-42138	CC	denotes	or
T20343	42139-42152	JJ	denotes	cysteine-rich
T20344	42153-42160	NN	denotes	domains
T20345	42160-42161	-COMMA-	denotes	,
T20346	42162-42174	VB	denotes	up-regulated
T20347	42175-42177	FW	denotes	in
T20348	42178-42182	FW	denotes	vivo
T20349	42183-42186	DT	denotes	the
T20350	42187-42197	NN	denotes	expression
T20351	42198-42200	IN	denotes	of
T20352	42201-42202	DT	denotes	a
T20353	42203-42209	NN	denotes	number
T20354	42210-42212	IN	denotes	of
T20355	42213-42229	JJ	denotes	NF-κB-responsive
T20356	42230-42235	NN	denotes	genes
T20357	42235-42236	-COMMA-	denotes	,
T20358	42237-42246	VB	denotes	including
T20359	42247-42253	NN	denotes	MIP-1α
T20360	42253-42254	-COMMA-	denotes	,
T20361	42255-42259	NN	denotes	CSF3
T20362	42259-42260	-COMMA-	denotes	,
T20363	42261-42264	NN	denotes	LTA
T20364	42264-42265	-COMMA-	denotes	,
T20365	42266-42272	NN	denotes	NFKBIA
T20366	42273-42276	CC	denotes	and
T20367	42277-42281	NN	denotes	TLR2
T20368	42283-42285	IN	denotes	By
T20369	42286-42290	NN	denotes	ChIP
T20370	42291-42299	NN	denotes	analysis
T20371	42299-42300	-COMMA-	denotes	,
T20372	42301-42303	PRP	denotes	we
T20373	42304-42312	VB	denotes	observed
T20374	42313-42317	IN	denotes	that
T20375	42318-42321	NN	denotes	Tat
T20376	42322-42331	VB	denotes	increased
T20377	42332-42335	DT	denotes	the
T20378	42336-42347	NN	denotes	recruitment
T20379	42348-42350	IN	denotes	of
T20380	42351-42354	NN	denotes	p65
T20381	42355-42357	TO	denotes	to
T20382	42358-42361	DT	denotes	the
T20383	42362-42367	NN	denotes	NF-κB
T20384	42368-42377	NN	denotes	enhancers
T20385	42378-42380	IN	denotes	of
T20386	42381-42384	DT	denotes	the
T20387	42385-42394	VB	denotes	activated
T20388	42395-42400	NN	denotes	genes
T20389	42400-42401	-COMMA-	denotes	,
T20390	42402-42405	CC	denotes	and
T20391	42406-42409	VB	denotes	was
T20392	42410-42419	VB	denotes	recovered
T20393	42420-42422	IN	denotes	at
T20394	42423-42426	DT	denotes	the
T20395	42427-42431	JJ	denotes	same
T20396	42432-42437	NN	denotes	sites
T20397	42439-42442	NN	denotes	RNA
T20398	42443-42455	NN	denotes	interference
T20399	42456-42458	IN	denotes	of
T20400	42459-42462	NN	denotes	p65
T20401	42463-42472	VB	denotes	abolished
T20402	42473-42476	DT	denotes	the
T20403	42477-42480	NN	denotes	Tat
T20404	42481-42490	NN	denotes	occupancy
T20405	42491-42493	IN	denotes	of
T20406	42494-42497	DT	denotes	the
T20407	42498-42503	NN	denotes	NF-κB
T20408	42504-42513	NN	denotes	enhancers
T20409	42514-42524	VB	denotes	indicating
T20410	42525-42529	IN	denotes	that
T20411	42530-42533	DT	denotes	the
T20412	42534-42537	NN	denotes	Tat
T20413	42538-42545	NN	denotes	binding
T20414	42546-42548	TO	denotes	to
T20415	42549-42552	DT	denotes	the
T20416	42553-42558	NN	denotes	NF-κB
T20417	42559-42564	NN	denotes	sites
T20418	42565-42568	VB	denotes	was
T20419	42569-42577	VB	denotes	mediated
T20420	42578-42580	IN	denotes	by
T20421	42581-42584	NN	denotes	p65
T20422	42586-42591	DT	denotes	These
T20423	42592-42599	NN	denotes	results
T20424	42600-42604	VB	denotes	were
T20425	42605-42615	JJ	denotes	consistent
T20426	42616-42620	IN	denotes	with
T20427	42621-42624	DT	denotes	the
T20428	42625-42633	NN	denotes	evidence
T20429	42634-42638	IN	denotes	that
T20430	42639-42642	NN	denotes	Tat
T20431	42643-42652	VB	denotes	displaced
T20432	42653-42656	NN	denotes	p65
T20433	42657-42661	IN	denotes	from
T20434	42662-42665	DT	denotes	the
T20435	42666-42673	NN	denotes	binding
T20436	42674-42676	TO	denotes	to
T20437	42677-42682	NN	denotes	IκB-α
T20438	42683-42684	-LRB-	denotes	(
T20439	42684-42691	NN	denotes	Figures
T20440	42692-42694	NN	denotes	2D
T20441	42695-42698	CC	denotes	and
T20442	42699-42701	NN	denotes	3C
T20443	42701-42702	-RRB-	denotes	)
T20444	42702-42703	-COMMA-	denotes	,
T20445	42704-42707	CC	denotes	and
T20446	42708-42717	VB	denotes	increased
T20447	42718-42721	DT	denotes	the
T20448	42722-42725	NN	denotes	p65
T20449	42726-42729	NN	denotes	DNA
T20450	42730-42737	NN	denotes	binding
T20451	42738-42746	NN	denotes	affinity
T20452	42747-42754	IN	denotes	through
T20453	42755-42766	NN	denotes	association
T20454	42767-42768	-LRB-	denotes	(
T20455	42768-42774	NN	denotes	Figure
T20456	42775-42777	NN	denotes	4E
T20457	42778-42781	CC	denotes	and
T20458	42782-42783	NN	denotes	F
T20459	42783-42784	-RRB-	denotes	)
T20460	42786-42790	RB	denotes	Thus
T20461	42790-42791	-COMMA-	denotes	,
T20462	42792-42795	NN	denotes	Tat
T20463	42796-42802	RB	denotes	likely
T20464	42803-42812	VB	denotes	increased
T20465	42813-42816	DT	denotes	the
T20466	42817-42828	NN	denotes	recruitment
T20467	42829-42831	IN	denotes	of
T20468	42832-42835	NN	denotes	p65
T20469	42836-42838	TO	denotes	to
T20470	42839-42842	DT	denotes	the
T20471	42843-42858	JJ	denotes	NF-κB-dependent
T20472	42859-42868	NN	denotes	promoters
T20473	42869-42871	IN	denotes	by
T20474	42872-42883	VB	denotes	associating
T20475	42884-42888	IN	denotes	with
T20476	42889-42892	NN	denotes	p65
T20477	42893-42896	CC	denotes	and
T20478	42897-42899	IN	denotes	by
T20479	42900-42911	VB	denotes	interfering
T20480	42912-42916	IN	denotes	with
T20481	42917-42920	DT	denotes	the
T20482	42921-42929	NN	denotes	assembly
T20483	42930-42932	IN	denotes	of
T20484	42933-42936	NN	denotes	p65
T20485	42937-42941	IN	denotes	with
T20486	42942-42945	DT	denotes	the
T20487	42946-42955	NN	denotes	repressor
T20488	42956-42961	NN	denotes	IκB-α
T20489	42963-42965	IN	denotes	Of
T20490	42966-42974	NN	denotes	interest
T20491	42974-42975	-COMMA-	denotes	,
T20492	42976-42981	NN	denotes	IκB-α
T20493	42982-42985	VB	denotes	was
T20494	42986-42991	VB	denotes	found
T20495	42992-43002	VB	denotes	associated
T20496	43003-43005	TO	denotes	to
T20497	43006-43009	DT	denotes	the
T20498	43010-43015	NN	denotes	NF-κB
T20499	43016-43025	NN	denotes	enhancers
T20500	43026-43028	IN	denotes	in
T20501	43029-43036	NN	denotes	absence
T20502	43037-43039	IN	denotes	of
T20503	43040-43043	NN	denotes	Tat
T20504	43043-43044	-COMMA-	denotes	,
T20505	43045-43050	WRB	denotes	where
T20506	43051-43053	PRP	denotes	it
T20507	43054-43057	VB	denotes	was
T20508	43058-43067	VB	denotes	displaced
T20509	43068-43070	IN	denotes	in
T20510	43071-43079	NN	denotes	presence
T20511	43080-43082	IN	denotes	of
T20512	43083-43087	NNP	denotes	Tat.
T20513	43088-43093	DT	denotes	These
T20514	43094-43101	NN	denotes	results
T20515	43102-43109	VB	denotes	suggest
T20516	43110-43111	DT	denotes	a
T20517	43112-43120	JJ	denotes	negative
T20518	43121-43131	JJ	denotes	regulatory
T20519	43132-43136	NN	denotes	role
T20520	43137-43139	IN	denotes	of
T20521	43140-43145	NN	denotes	IκB-α
T20522	43146-43148	IN	denotes	in
T20523	43149-43153	NN	denotes	gene
T20524	43154-43164	NN	denotes	regulation
T20525	43165-43172	IN	denotes	through
T20526	43173-43182	NN	denotes	occupancy
T20527	43183-43185	IN	denotes	of
T20528	43186-43194	JJ	denotes	specific
T20529	43195-43204	NN	denotes	promoters
T20530	43206-43212	RB	denotes	Indeed
T20531	43212-43213	-COMMA-	denotes	,
T20532	43214-43219	NN	denotes	IκB-α
T20533	43220-43223	VB	denotes	was
T20534	43224-43229	VB	denotes	found
T20535	43230-43240	VB	denotes	associated
T20536	43241-43245	IN	denotes	with
T20537	43246-43253	NN	denotes	hystone
T20538	43254-43266	NN	denotes	deacetylases
T20539	43267-43269	IN	denotes	at
T20540	43270-43273	DT	denotes	the
T20541	43274-43278	NN	denotes	hes1
T20542	43279-43287	NN	denotes	promoter
T20543	43287-43288	-COMMA-	denotes	,
T20544	43289-43293	IN	denotes	from
T20545	43294-43299	WRB	denotes	where
T20546	43300-43302	PRP	denotes	it
T20547	43303-43306	VB	denotes	was
T20548	43307-43314	VB	denotes	removed
T20549	43315-43324	VB	denotes	following
T20550	43325-43330	NN	denotes	TNF-α
T20551	43331-43342	NN	denotes	stimulation
T20552	43343-43350	VB	denotes	causing
T20553	43351-43358	NN	denotes	histone
T20554	43359-43370	NN	denotes	acetylation
T20555	43371-43374	CC	denotes	and
T20556	43375-43379	NN	denotes	hes1
T20557	43380-43395	JJ	denotes	transcriptional
T20558	43396-43406	NN	denotes	activation
T20559	43407-43408	-LRB-	denotes	(
T20560	43408-43410	CD	denotes	65
T20561	43410-43411	-RRB-	denotes	)
T20562	43413-43414	DT	denotes	A
T20563	43415-43422	JJ	denotes	similar
T20564	43423-43432	NN	denotes	mechanism
T20565	43433-43435	IN	denotes	of
T20566	43436-43440	NN	denotes	gene
T20567	43441-43451	NN	denotes	regulation
T20568	43452-43457	MD	denotes	could
T20569	43458-43463	VB	denotes	apply
T20570	43464-43466	TO	denotes	to
T20571	43467-43470	DT	denotes	the
T20572	43471-43486	JJ	denotes	NF-κB-dependent
T20573	43487-43492	NN	denotes	genes
T20574	43493-43501	VB	denotes	analysed
T20575	43502-43504	IN	denotes	in
T20576	43505-43509	DT	denotes	this
T20577	43510-43515	NN	denotes	study
T20578	43515-43516	-COMMA-	denotes	,
T20579	43517-43522	WRB	denotes	where
T20580	43523-43526	NN	denotes	Tat
T20581	43527-43532	MD	denotes	could
T20582	43533-43541	VB	denotes	activate
T20583	43542-43545	DT	denotes	the
T20584	43546-43550	NN	denotes	gene
T20585	43551-43561	NN	denotes	expression
T20586	43562-43564	IN	denotes	by
T20587	43565-43573	VB	denotes	removing
T20588	43574-43579	NN	denotes	IκB-α
T20589	43580-43583	CC	denotes	and
T20590	43584-43593	VB	denotes	promoting
T20591	43594-43597	DT	denotes	the
T20592	43598-43601	NN	denotes	p65
T20593	43602-43609	NN	denotes	loading
T20594	43610-43611	-LRB-	denotes	(
T20595	43611-43617	NN	denotes	Figure
T20596	43618-43619	CD	denotes	7
T20597	43619-43620	-RRB-	denotes	)
T20598	44045-44048	DT	denotes	The
T20599	44049-44062	JJ	denotes	physiological
T20600	44063-44072	NN	denotes	relevance
T20601	44073-44075	IN	denotes	of
T20602	44076-44079	NN	denotes	Tat
T20603	44080-44085	RB	denotes	cross
T20604	44086-44090	NN	denotes	talk
T20605	44091-44095	IN	denotes	with
T20606	44096-44101	NN	denotes	NF-κB
T20607	44102-44105	VB	denotes	was
T20608	44106-44118	VB	denotes	demonstrated
T20609	44119-44121	IN	denotes	in
T20610	44122-44125	DT	denotes	the
T20611	44126-44131	NN	denotes	HIV-1
T20612	44132-44141	NN	denotes	infection
T20613	44142-44144	IN	denotes	of
T20614	44145-44150	JJ	denotes	human
T20615	44151-44160	NN	denotes	monocytes
T20616	44160-44161	-COMMA-	denotes	,
T20617	44162-44167	WRB	denotes	where
T20618	44168-44181	JJ	denotes	HIV-1-encoded
T20619	44182-44185	NN	denotes	Tat
T20620	44186-44193	NN	denotes	protein
T20621	44194-44206	VB	denotes	counteracted
T20622	44207-44222	JJ	denotes	post-activation
T20623	44223-44227	NN	denotes	turn
T20624	44228-44231	RP	denotes	off
T20625	44232-44234	IN	denotes	of
T20626	44235-44240	NN	denotes	NF-κB
T20627	44240-44241	-COMMA-	denotes	,
T20628	44242-44244	IN	denotes	as
T20629	44245-44250	VB	denotes	shown
T20630	44251-44253	IN	denotes	by
T20631	44253-44254	-COLON-	denotes	:
T20632	44255-44256	-LRB-	denotes	(
T20633	44256-44257	LS	denotes	i
T20634	44257-44258	-RRB-	denotes	)
T20635	44259-44268	VB	denotes	sustained
T20636	44269-44274	NN	denotes	NF-κB
T20637	44275-44283	NN	denotes	activity
T20638	44284-44286	IN	denotes	by
T20639	44287-44290	NN	denotes	Tat
T20640	44291-44293	IN	denotes	in
T20641	44294-44302	NN	denotes	presence
T20642	44303-44305	IN	denotes	of
T20643	44306-44311	RB	denotes	newly
T20644	44312-44323	VB	denotes	synthesized
T20645	44324-44329	NN	denotes	IκB-α
T20646	44329-44330	-COLON-	denotes	;
T20647	44331-44332	-LRB-	denotes	(
T20648	44332-44334	LS	denotes	ii
T20649	44334-44335	-RRB-	denotes	)
T20650	44336-44357	NN	denotes	coimmunoprecipitation
T20651	44358-44360	IN	denotes	of
T20652	44361-44364	NN	denotes	Tat
T20653	44365-44369	IN	denotes	with
T20654	44370-44375	NN	denotes	IκB-α
T20655	44376-44379	CC	denotes	and
T20656	44380-44383	NN	denotes	p65
T20657	44383-44384	-COLON-	denotes	;
T20658	44385-44386	-LRB-	denotes	(
T20659	44386-44389	LS	denotes	iii
T20660	44389-44390	-RRB-	denotes	)
T20661	44391-44400	NN	denotes	induction
T20662	44401-44403	IN	denotes	of
T20663	44404-44410	NN	denotes	MIP-1α
T20664	44411-44421	NN	denotes	expression
T20665	44422-44431	JJ	denotes	dependent
T20666	44432-44434	IN	denotes	on
T20667	44435-44438	NN	denotes	Tat
T20668	44439-44442	CC	denotes	and
T20669	44443-44446	NN	denotes	p65
T20670	44446-44447	-COLON-	denotes	;
T20671	44448-44449	-LRB-	denotes	(
T20672	44449-44451	LS	denotes	iv
T20673	44451-44452	-RRB-	denotes	)
T20674	44453-44456	NN	denotes	Tat
T20675	44457-44466	NN	denotes	occupancy
T20676	44467-44469	IN	denotes	of
T20677	44470-44473	DT	denotes	the
T20678	44474-44480	NN	denotes	MIP-1α
T20679	44481-44486	NN	denotes	NF-κB
T20680	44487-44495	NN	denotes	enhancer
T20681	44496-44506	VB	denotes	associated
T20682	44507-44511	IN	denotes	with
T20683	44512-44521	VB	denotes	increased
T20684	44522-44533	NN	denotes	recruitment
T20685	44534-44536	IN	denotes	of
T20686	44537-44540	NN	denotes	p65
T20687	44542-44546	DT	denotes	This
T20688	44547-44552	NN	denotes	study
T20689	44553-44561	VB	denotes	supports
T20690	44562-44565	DT	denotes	the
T20691	44566-44582	JJ	denotes	pro-inflammatory
T20692	44583-44589	NN	denotes	action
T20693	44590-44592	IN	denotes	of
T20694	44593-44596	NN	denotes	Tat
T20695	44597-44604	IN	denotes	through
T20696	44605-44613	JJ	denotes	physical
T20697	44614-44625	NN	denotes	interaction
T20698	44626-44630	IN	denotes	with
T20699	44631-44634	NN	denotes	p65
T20700	44635-44638	CC	denotes	and
T20701	44639-44644	NN	denotes	IκB-α
T20702	44646-44653	JJ	denotes	Several
T20703	44654-44666	JJ	denotes	inflammatory
T20704	44667-44676	NN	denotes	cytokines
T20705	44677-44682	IN	denotes	under
T20706	44683-44686	DT	denotes	the
T20707	44687-44702	JJ	denotes	transcriptional
T20708	44703-44710	NN	denotes	control
T20709	44711-44713	IN	denotes	of
T20710	44714-44719	NN	denotes	NF-κB
T20711	44720-44723	VB	denotes	are
T20712	44724-44739	JJ	denotes	hyper-expressed
T20713	44740-44742	IN	denotes	in
T20714	44743-44748	NN	denotes	HIV-1
T20715	44749-44757	JJ	denotes	infected
T20716	44758-44769	NN	denotes	individuals
T20717	44769-44770	-COMMA-	denotes	,
T20718	44771-44780	VB	denotes	including
T20719	44781-44785	NN	denotes	IL-6
T20720	44786-44787	-LRB-	denotes	(
T20721	44787-44792	CD	denotes	66,67
T20722	44792-44793	-RRB-	denotes	)
T20723	44793-44794	-COMMA-	denotes	,
T20724	44795-44800	NN	denotes	TNF-α
T20725	44801-44802	-LRB-	denotes	(
T20726	44802-44804	CD	denotes	68
T20727	44804-44805	-RRB-	denotes	)
T20728	44805-44806	-COMMA-	denotes	,
T20729	44807-44810	CC	denotes	and
T20730	44811-44817	NN	denotes	MIP-1α
T20731	44818-44819	-LRB-	denotes	(
T20732	44819-44830	CD	denotes	57–62,69–71
T20733	44830-44831	-RRB-	denotes	)
T20734	44833-44835	IN	denotes	In
T20735	44836-44839	DT	denotes	the
T20736	44840-44844	NN	denotes	case
T20737	44845-44847	IN	denotes	of
T20738	44848-44853	NN	denotes	TNF-α
T20739	44854-44857	CC	denotes	and
T20740	44858-44862	NN	denotes	IL-6
T20741	44862-44863	-COMMA-	denotes	,
T20742	44864-44867	NN	denotes	Tat
T20743	44868-44877	VB	denotes	activated
T20744	44878-44881	DT	denotes	the
T20745	44882-44890	NN	denotes	cytokine
T20746	44891-44901	NN	denotes	expression
T20747	44902-44904	IN	denotes	by
T20748	44905-44912	VB	denotes	binding
T20749	44913-44915	TO	denotes	to
T20750	44916-44919	DT	denotes	the
T20751	44920-44928	JJ	denotes	TAR-like
T20752	44929-44933	NN	denotes	stem
T20753	44934-44938	NN	denotes	loop
T20754	44939-44941	IN	denotes	of
T20755	44942-44945	DT	denotes	the
T20756	44946-44948	NN	denotes	5′
T20757	44949-44959	NN	denotes	transcript
T20758	44959-44960	-COMMA-	denotes	,
T20759	44961-44965	RB	denotes	thus
T20760	44966-44972	RB	denotes	likely
T20761	44973-44982	VB	denotes	promoting
T20762	44983-44986	DT	denotes	the
T20763	44987-45002	JJ	denotes	transcriptional
T20764	45003-45013	NN	denotes	elongation
T20765	45014-45015	-LRB-	denotes	(
T20766	45015-45023	CD	denotes	33,37,47
T20767	45023-45024	-RRB-	denotes	)
T20768	45026-45030	RB	denotes	Here
T20769	45030-45031	-COMMA-	denotes	,
T20770	45032-45034	PRP	denotes	we
T20771	45035-45039	VB	denotes	have
T20772	45040-45045	VB	denotes	shown
T20773	45046-45050	IN	denotes	that
T20774	45051-45054	DT	denotes	the
T20775	45055-45068	JJ	denotes	Tat-dependent
T20776	45069-45084	JJ	denotes	transcriptional
T20777	45085-45095	NN	denotes	activation
T20778	45096-45098	IN	denotes	of
T20779	45099-45100	DT	denotes	a
T20780	45101-45107	NN	denotes	number
T20781	45108-45110	IN	denotes	of
T20782	45111-45127	JJ	denotes	pro-inflammatory
T20783	45128-45133	NN	denotes	genes
T20784	45134-45142	VB	denotes	required
T20785	45143-45146	DT	denotes	the
T20786	45147-45155	NN	denotes	assembly
T20787	45156-45158	IN	denotes	of
T20788	45159-45162	NN	denotes	Tat
T20789	45163-45167	IN	denotes	with
T20790	45168-45171	NN	denotes	p65
T20791	45172-45174	IN	denotes	at
T20792	45175-45178	DT	denotes	the
T20793	45179-45184	NN	denotes	NF-κB
T20794	45185-45193	NN	denotes	enhancer
T20795	45194-45202	RB	denotes	together
T20796	45203-45207	IN	denotes	with
T20797	45208-45211	DT	denotes	the
T20798	45212-45224	NN	denotes	displacement
T20799	45225-45227	IN	denotes	of
T20800	45228-45233	NN	denotes	IκB-α
T20801	45234-45235	-LRB-	denotes	(
T20802	45235-45241	NN	denotes	Figure
T20803	45242-45243	CD	denotes	5
T20804	45243-45244	-RRB-	denotes	)
T20805	45246-45250	RB	denotes	Thus
T20806	45250-45251	-COMMA-	denotes	,
T20807	45252-45254	IN	denotes	in
T20808	45255-45258	DT	denotes	the
T20809	45259-45262	NN	denotes	set
T20810	45263-45265	IN	denotes	of
T20811	45266-45274	JJ	denotes	distinct
T20812	45275-45291	JJ	denotes	NF-κB-responsive
T20813	45292-45297	NN	denotes	genes
T20814	45297-45298	-COMMA-	denotes	,
T20815	45299-45302	NN	denotes	Tat
T20816	45303-45308	VB	denotes	acted
T20817	45309-45311	IN	denotes	as
T20818	45312-45313	DT	denotes	a
T20819	45314-45323	NN	denotes	component
T20820	45324-45326	IN	denotes	of
T20821	45327-45330	DT	denotes	the
T20822	45331-45346	JJ	denotes	transcriptional
T20823	45347-45357	NN	denotes	initiation
T20824	45358-45365	NN	denotes	complex
T20825	45365-45366	-COMMA-	denotes	,
T20826	45367-45372	WDT	denotes	which
T20827	45373-45375	VB	denotes	is
T20828	45376-45386	JJ	denotes	consistent
T20829	45387-45391	IN	denotes	with
T20830	45392-45400	JJ	denotes	previous
T20831	45401-45408	NN	denotes	reports
T20832	45409-45411	IN	denotes	on
T20833	45412-45415	NN	denotes	Tat
T20834	45416-45425	VB	denotes	promoting
T20835	45426-45429	DT	denotes	the
T20836	45430-45443	NN	denotes	transcription
T20837	45444-45447	IN	denotes	via
T20838	45448-45451	NN	denotes	DNA
T20839	45452-45458	NN	denotes	motifs
T20840	45459-45460	-LRB-	denotes	(
T20841	45460-45465	CD	denotes	72,73
T20842	45465-45466	-RRB-	denotes	)
T20843	45468-45482	NN	denotes	Overproduction
T20844	45483-45485	IN	denotes	of
T20845	45486-45502	JJ	denotes	pro-inflammatory
T20846	45503-45512	NN	denotes	cytokines
T20847	45513-45515	VB	denotes	is
T20848	45516-45526	VB	denotes	considered
T20849	45527-45528	DT	denotes	a
T20850	45529-45534	JJ	denotes	major
T20851	45535-45544	NN	denotes	mechanism
T20852	45545-45547	IN	denotes	of
T20853	45548-45554	JJ	denotes	immune
T20854	45555-45567	NN	denotes	deregulation
T20855	45568-45569	-LRB-	denotes	(
T20856	45569-45571	CD	denotes	26
T20857	45571-45572	-RRB-	denotes	)
T20858	45573-45576	CC	denotes	and
T20859	45577-45583	NN	denotes	neuron
T20860	45584-45595	NN	denotes	dysfunction
T20861	45596-45598	IN	denotes	in
T20862	45599-45603	NN	denotes	AIDS
T20863	45604-45605	-LRB-	denotes	(
T20864	45605-45610	CD	denotes	74,75
T20865	45610-45611	-RRB-	denotes	)
T20866	45613-45615	IN	denotes	In
T20867	45616-45620	DT	denotes	this
T20868	45621-45629	NN	denotes	scenario
T20869	45629-45630	-COMMA-	denotes	,
T20870	45631-45641	NN	denotes	inhibition
T20871	45642-45644	IN	denotes	of
T20872	45645-45650	NN	denotes	NF-κB
T20873	45651-45659	NN	denotes	activity
T20874	45660-45665	MD	denotes	could
T20875	45666-45670	VB	denotes	lead
T20876	45671-45673	TO	denotes	to
T20877	45674-45675	DT	denotes	a
T20878	45676-45687	JJ	denotes	therapeutic
T20879	45688-45696	NN	denotes	strategy
T20880	45697-45700	IN	denotes	for
T20881	45701-45714	VB	denotes	counteracting
T20882	45715-45718	DT	denotes	the
T20883	45719-45727	JJ	denotes	abnormal
T20884	45728-45738	NN	denotes	production
T20885	45739-45741	IN	denotes	of
T20886	45742-45758	JJ	denotes	pro-inflammatory
T20887	45759-45768	NN	denotes	cytokines
T20888	45769-45772	CC	denotes	and
T20889	45773-45783	NN	denotes	chemokines
T20890	45784-45786	IN	denotes	in
T20891	45787-45792	NN	denotes	HIV-1
T20892	45793-45802	NN	denotes	infection
T20893	45804-45809	NN	denotes	NF-κB
T20894	45810-45820	NN	denotes	inhibitors
T20895	45820-45821	-COMMA-	denotes	,
T20896	45822-45826	JJ	denotes	such
T20897	45827-45829	IN	denotes	as
T20898	45830-45843	NN	denotes	anti-oxidants
T20899	45843-45844	-COMMA-	denotes	,
T20900	45845-45855	NN	denotes	proteasome
T20901	45856-45859	CC	denotes	and
T20902	45860-45863	NN	denotes	IKK
T20903	45864-45874	NN	denotes	inhibitors
T20904	45874-45875	-COMMA-	denotes	,
T20905	45876-45889	RB	denotes	significantly
T20906	45890-45897	VB	denotes	reduced
T20907	45898-45901	DT	denotes	the
T20908	45902-45907	NN	denotes	HIV-1
T20909	45908-45919	NN	denotes	replication
T20910	45920-45923	CC	denotes	and
T20911	45924-45927	DT	denotes	the
T20912	45928-45938	VB	denotes	associated
T20913	45939-45951	JJ	denotes	inflammatory
T20914	45952-45960	NN	denotes	response
T20915	45961-45962	-LRB-	denotes	(
T20916	45962-45967	CD	denotes	76,77
T20917	45967-45968	-RRB-	denotes	)
T20918	45968-45969	-COLON-	denotes	;
T20919	45970-45977	RB	denotes	however
T20920	45977-45978	-COMMA-	denotes	,
T20921	45979-45984	DT	denotes	these
T20922	45985-45990	NN	denotes	NF-κB
T20923	45991-46001	NN	denotes	inhibitors
T20924	46002-46007	VB	denotes	acted
T20925	46008-46024	RB	denotes	indiscriminately
T20926	46025-46027	IN	denotes	in
T20927	46028-46032	CC	denotes	both
T20928	46033-46047	JJ	denotes	HIV-1-infected
T20929	46048-46051	CC	denotes	and
T20930	46052-46062	JJ	denotes	uninfected
T20931	46063-46068	NN	denotes	cells
T20932	46070-46073	DT	denotes	The
T20933	46074-46082	NN	denotes	evidence
T20934	46083-46087	IN	denotes	that
T20935	46088-46091	NN	denotes	Tat
T20936	46092-46101	VB	denotes	activates
T20937	46102-46107	NN	denotes	NF-κB
T20938	46108-46115	IN	denotes	through
T20939	46116-46122	JJ	denotes	direct
T20940	46123-46134	NN	denotes	interaction
T20941	46135-46139	IN	denotes	with
T20942	46140-46145	NN	denotes	IκB-α
T20943	46146-46149	CC	denotes	and
T20944	46150-46153	NN	denotes	p65
T20945	46154-46157	MD	denotes	may
T20946	46158-46162	VB	denotes	lead
T20947	46163-46165	TO	denotes	to
T20948	46166-46174	JJ	denotes	specific
T20949	46175-46185	NN	denotes	inhibitors
T20950	46186-46188	TO	denotes	to
T20951	46189-46199	VB	denotes	counteract
T20952	46200-46203	DT	denotes	the
T20953	46204-46207	NN	denotes	Tat
T20954	46208-46224	JJ	denotes	pro-inflammatory
T20955	46225-46231	NN	denotes	action
T24359	20207-20210	NN	denotes	Tat
T24360	20211-20219	VB	denotes	enhances
T24361	20220-20223	DT	denotes	the
T24362	20224-20229	NN	denotes	NF-κB
T24363	20230-20238	NN	denotes	activity
T24364	20239-20248	VB	denotes	following
T24365	20249-20252	NN	denotes	PMA
T24366	20253-20264	NN	denotes	stimulation
T24367	20265-20267	IN	denotes	by
T24368	20268-20279	VB	denotes	associating
T24369	20280-20284	IN	denotes	with
T24370	20285-20290	NN	denotes	IκB-α
T24371	20291-20294	CC	denotes	and
T24372	20295-20308	VB	denotes	counteracting
T24373	20309-20312	DT	denotes	the
T24374	20313-20318	NN	denotes	NF-κB
T24375	20319-20329	NN	denotes	repression
T24376	20331-20335	NN	denotes	HeLa
T24377	20336-20341	NN	denotes	cells
T24378	20342-20343	-LRB-	denotes	(
T24379	20343-20350	CD	denotes	5 × 106
T24380	20350-20351	-RRB-	denotes	)
T24381	20352-20356	VB	denotes	were
T24382	20357-20368	VB	denotes	transfected
T24383	20369-20373	IN	denotes	with
T24384	20374-20385	NN	denotes	p3xFLAG-Tat
T24385	20386-20388	CC	denotes	or
T24386	20389-20396	NN	denotes	p3xFLAG
T24387	20397-20402	NN	denotes	empty
T24388	20403-20409	NN	denotes	vector
T24389	20410-20411	-LRB-	denotes	(
T24390	20411-20415	NN	denotes	5 µg
T24391	20415-20416	-RRB-	denotes	)
T24392	20416-20417	-COMMA-	denotes	,
T24393	20418-20421	CC	denotes	and
T24394	20422-20426	NN	denotes	48 h
T24395	20427-20432	RB	denotes	later
T24396	20433-20437	VB	denotes	were
T24397	20438-20448	VB	denotes	stimulated
T24398	20449-20453	IN	denotes	with
T24399	20454-20457	NN	denotes	PMA
T24400	20458-20459	-LRB-	denotes	(
T24401	20459-20464	NN	denotes	5 min
T24402	20464-20465	-COMMA-	denotes	,
T24403	20466-20474	NN	denotes	20 ng/ml
T24404	20474-20475	-RRB-	denotes	)
T24405	20476-20478	CC	denotes	or
T24406	20479-20483	VB	denotes	left
T24407	20484-20496	JJ	denotes	unstimulated
T24408	20496-20497	-COMMA-	denotes	,
T24409	20498-20504	VB	denotes	washed
T24410	20505-20510	RB	denotes	twice
T24411	20511-20515	IN	denotes	with
T24412	20516-20520	NN	denotes	DMEM
T24413	20521-20524	CC	denotes	and
T24414	20525-20534	VB	denotes	harvested
T24415	20535-20537	IN	denotes	at
T24416	20538-20541	DT	denotes	the
T24417	20542-20551	JJ	denotes	indicated
T24418	20552-20557	NN	denotes	times
T24419	20559-20566	JJ	denotes	Nuclear
T24420	20567-20570	CC	denotes	and
T24421	20571-20580	JJ	denotes	cytosolic
T24422	20581-20589	NN	denotes	extracts
T24423	20590-20594	VB	denotes	were
T24424	20595-20603	VB	denotes	prepared
T24425	20604-20607	IN	denotes	for
T24426	20608-20615	JJ	denotes	further
T24427	20616-20625	NN	denotes	analysis.
T24428	20626-20627	-LRB-	denotes	(
T24429	20627-20628	NN	denotes	A
T24430	20628-20629	-RRB-	denotes	)
T24431	20630-20637	JJ	denotes	Nuclear
T24432	20638-20646	NN	denotes	extracts
T24433	20647-20651	VB	denotes	were
T24434	20652-20660	VB	denotes	analysed
T24435	20661-20664	IN	denotes	for
T24436	20665-20668	DT	denotes	the
T24437	20669-20672	NN	denotes	p65
T24438	20672-20673	-COMMA-	denotes	,
T24439	20674-20677	NN	denotes	p50
T24440	20678-20681	CC	denotes	and
T24441	20682-20690	NN	denotes	FLAG-Tat
T24442	20691-20698	NN	denotes	binding
T24443	20699-20701	TO	denotes	to
T24444	20702-20705	DT	denotes	the
T24445	20706-20711	NN	denotes	NF-κB
T24446	20712-20727	JJ	denotes	double-stranded
T24447	20728-20743	NN	denotes	oligonucleotide
T24448	20744-20749	VB	denotes	using
T24449	20750-20753	DT	denotes	the
T24450	20754-20759	NN	denotes	NF-κB
T24451	20760-20773	NN	denotes	Transcription
T24452	20774-20780	NN	denotes	Factor
T24453	20781-20786	NN	denotes	ELISA
T24454	20787-20792	NN	denotes	assay
T24455	20793-20796	NN	denotes	kit
T24456	20797-20798	-LRB-	denotes	(
T24457	20798-20804	NN	denotes	Cayman
T24458	20804-20805	-RRB-	denotes	)
T24459	20805-20806	-LRB-	denotes	.
T24460	20807-20808	NN	denotes	(
T24461	20808-20809	-RRB-	denotes	B
T24462	20809-20816	JJ	denotes	) Nucle
T24463	20816-20819	CC	denotes	ar
T24464	20819-20828	JJ	denotes	and cytos
T24465	20828-20836	NN	denotes	olic ext
T24466	20836-20837	-LRB-	denotes	r
T24467	20837-20842	NN	denotes	acts
T24468	20842-20843	-RRB-	denotes	(
T24469	20843-20847	VB	denotes	20 µ
T24470	20847-20856	VB	denotes	g) were s
T24471	20856-20858	IN	denotes	ep
T24472	20858-20860	CD	denotes	ar
T24473	20860-20861	NN	denotes	a
T24474	20861-20869	NN	denotes	ted by 1
T24475	20869-20872	CC	denotes	2%
T24476	20872-20880	VB	denotes	SDS–PAGE
T24477	20881-20883	IN	denotes	an
T24478	20883-20890	JJ	denotes	d analy
T24479	20890-20898	NN	denotes	sed by w
T24480	20898-20903	VB	denotes	ester
T24481	20903-20906	DT	denotes	n b
T24482	20906-20914	JJ	denotes	lotting
T24483	20914-20915	-COMMA-	denotes	u
T24484	20915-20924	JJ	denotes	sing the
T24485	20924-20925	-COMMA-	denotes	a
T24486	20925-20937	JJ	denotes	nti-p65, ant
T24487	20937-20939	NN	denotes	i-
T24488	20939-20940	-COMMA-	denotes	F
T24489	20940-20950	JJ	denotes	LAG, anti-
T24490	20950-20953	CC	denotes	His
T24491	20953-20971	JJ	denotes	tone H1, anti-IκB-
T24492	20971-20981	NN	denotes	α and anti
T24493	21008-21020	NN	denotes	Densitometry
T24494	21021-21027	NN	denotes	values
T24495	21028-21029	-LRB-	denotes	(
T24496	21029-21030	NN	denotes	D
T24497	21030-21031	-RRB-	denotes	)
T24498	21032-21034	IN	denotes	of
T24499	21035-21038	DT	denotes	the
T24500	21039-21044	NN	denotes	bands
T24501	21045-21049	VB	denotes	were
T24502	21050-21059	VB	denotes	expressed
T24503	21060-21062	IN	denotes	as
T24504	21063-21067	JJ	denotes	fold
T24505	21068-21076	NN	denotes	increase
T24506	21077-21082	IN	denotes	above
T24507	21083-21086	DT	denotes	the
T24508	21087-21094	NN	denotes	control
T24509	21095-21096	-LRB-	denotes	(
T24510	21096-21100	NN	denotes	lane
T24511	21101-21102	CD	denotes	1
T24512	21102-21103	-RRB-	denotes	)
T24513	21103-21104	-LRB-	denotes	.
T24514	21105-21106	NN	denotes	(
T24515	21106-21107	-RRB-	denotes	C
T24516	21107-21110	NN	denotes	) I
T24517	21110-21118	NN	denotes	KK activ
T24518	21118-21121	VB	denotes	ity
T24519	21122-21130	VB	denotes	was meas
T24520	21130-21132	IN	denotes	ur
T24521	21132-21141	JJ	denotes	ed in cyt
T24522	21141-21149	NN	denotes	osolic e
T24523	21149-21150	-LRB-	denotes	x
T24524	21150-21156	NN	denotes	tracts
T24525	21157-21158	-RRB-	denotes	(
T24526	21158-21160	IN	denotes	10
T24527	21160-21165	VB	denotes	0 µg)
T24528	21166-21172	NN	denotes	by usi
T24529	21172-21175	NN	denotes	ng
T24530	21175-21181	NN	denotes	HTScan
T24531	21182-21187	NN	denotes	IKK k
T24532	21187-21188	-LRB-	denotes	i
T24533	21188-21192	NNP	denotes	nase
T24534	21193-21202	NNP	denotes	assay (Ce
T24535	21202-21212	NNP	denotes	ll Signali
T24536	21212-21213	-RRB-	denotes	n
T24537	21213-21214	-LRB-	denotes	g
T24538	21215-21216	NN	denotes	T
T24539	21216-21217	-RRB-	denotes	e
T24540	21217-21221	NN	denotes	chno
T24541	21221-21226	NN	denotes	logy)
T24542	21226-21227	-LRB-	denotes	.
T24543	21228-21235	CD	denotes	(D) HeL
T24544	21235-21236	-RRB-	denotes	a
T24545	21237-21241	VB	denotes	cell
T24546	21241-21252	VB	denotes	s (5 × 106)
T24547	21253-21257	IN	denotes	were
T24548	21258-21260	CC	denotes	tr
T24549	21260-21267	IN	denotes	ansfect
T24550	21267-21278	NN	denotes	ed with or
T24551	21278-21279	-LRB-	denotes	w
T24552	21279-21283	NN	denotes	itho
T24553	21283-21284	-RRB-	denotes	u
T24554	21284-21285	-COLON-	denotes	t
T24555	21286-21290	NN	denotes	p3xF
T24556	21290-21295	RB	denotes	LAG-T
T24557	21295-21296	-COMMA-	denotes	a
T24558	21296-21301	NN	denotes	t (5
T24559	21301-21305	VB	denotes	µg);
T24560	21306-21316	VB	denotes	48 h later
T24561	21316-21320	IN	denotes	, ce
T24562	21320-21323	NN	denotes	lls
T24563	21324-21325	-LRB-	denotes	w
T24564	21325-21330	NN	denotes	ere s
T24565	21330-21331	-COMMA-	denotes	t
T24566	21331-21339	NN	denotes	imulated
T24567	21340-21341	-RRB-	denotes	w
T24568	21341-21343	CC	denotes	it
T24569	21343-21347	VB	denotes	h PM
T24570	21347-21359	JJ	denotes	A (5 min, 20
T24571	21359-21360	-COMMA-	denotes
T24572	21360-21366	VB	denotes	ng/ml)
T24573	21367-21372	RB	denotes	or le
T24574	21372-21376	IN	denotes	ft u
T24575	21376-21380	NN	denotes	nsti
T24576	21380-21381	-COMMA-	denotes	m
T24577	21381-21384	CC	denotes	ula
T24578	21384-21393	VB	denotes	ted, wash
T24579	21393-21398	IN	denotes	ed tw
T24580	21398-21405	NN	denotes	ice wit
T24581	21442-21447	JJ	denotes	Whole
T24582	21448-21452	NN	denotes	cell
T24583	21453-21461	NN	denotes	extracts
T24584	21462-21463	-LRB-	denotes	(
T24585	21463-21467	NN	denotes	1 mg
T24586	21467-21468	-RRB-	denotes	)
T24587	21469-21473	VB	denotes	were
T24588	21474-21492	VB	denotes	immunoprecipitated
T24589	21493-21497	IN	denotes	with
T24590	21498-21505	NN	denotes	protein
T24591	21506-21525	JJ	denotes	G-Sepharose-coupled
T24592	21526-21536	JJ	denotes	anti-IκB-α
T24593	21537-21545	NN	denotes	antibody
T24594	21547-21562	NN	denotes	Immunocomplexes
T24595	21563-21567	VB	denotes	were
T24596	21568-21577	VB	denotes	separated
T24597	21578-21580	IN	denotes	by
T24598	21581-21583	CD	denotes	12
T24599	21583-21584	NN	denotes	%
T24600	21585-21593	NN	denotes	SDS–PAGE
T24601	21594-21597	CC	denotes	and
T24602	21598-21606	VB	denotes	analysed
T24603	21607-21609	IN	denotes	by
T24604	21610-21617	JJ	denotes	western
T24605	21618-21626	NN	denotes	blotting
T24606	21627-21631	IN	denotes	with
T24607	21632-21640	JJ	denotes	anti-p65
T24608	21640-21641	-COMMA-	denotes	,
T24609	21642-21651	JJ	denotes	anti-FLAG
T24610	21652-21655	CC	denotes	and
T24611	21656-21666	JJ	denotes	anti-IκB-α
T24612	21667-21677	NN	denotes	antibodies
T24613	21679-21691	NN	denotes	Densitometry
T24614	21692-21698	NN	denotes	values
T24615	21699-21700	-LRB-	denotes	(
T24616	21700-21701	NN	denotes	D
T24617	21701-21702	-RRB-	denotes	)
T24618	21703-21705	IN	denotes	of
T24619	21706-21709	DT	denotes	the
T24620	21710-21715	NN	denotes	bands
T24621	21716-21720	VB	denotes	were
T24622	21721-21730	VB	denotes	expressed
T24623	21731-21733	IN	denotes	as
T24624	21734-21738	JJ	denotes	fold
T24625	21739-21747	NN	denotes	increase
T24626	21748-21753	IN	denotes	above
T24627	21754-21757	DT	denotes	the
T24628	21758-21765	NN	denotes	control
T24629	21766-21767	-LRB-	denotes	(
T24630	21767-21771	NN	denotes	lane
T24631	21772-21773	CD	denotes	1
T24632	21773-21774	-RRB-	denotes	)
T24633	21776-21782	NN	denotes	Values
T24634	21783-21784	-LRB-	denotes	(
T24635	21784-21793	NN	denotes	mean ± SE
T24636	21793-21794	-COMMA-	denotes	,
T24637	21795-21800	NN	denotes	n = 3
T24638	21800-21801	-RRB-	denotes	)
T24639	21802-21805	VB	denotes	are
T24640	21806-21811	VB	denotes	shown
T25214	23400-23403	NN	denotes	Tat
T25215	23404-23412	VB	denotes	relieves
T25216	23413-23416	NN	denotes	p65
T25217	23417-23421	IN	denotes	from
T25218	23422-23425	DT	denotes	the
T25219	23426-23431	JJ	denotes	IκB-α
T25220	23432-23443	NN	denotes	inhibition.
T25221	23444-23445	-LRB-	denotes	(
T25222	23445-23446	NN	denotes	A
T25223	23446-23447	-RRB-	denotes	)
T25224	23448-23457	JJ	denotes	Schematic
T25225	23458-23472	NN	denotes	representation
T25226	23473-23475	IN	denotes	of
T25227	23476-23485	JJ	denotes	wild-type
T25228	23486-23489	CC	denotes	and
T25229	23490-23496	JJ	denotes	mutant
T25230	23497-23500	NN	denotes	Tat
T25231	23501-23510	NN	denotes	proteins.
T25232	23511-23512	-LRB-	denotes	(
T25233	23512-23513	NN	denotes	B
T25234	23513-23514	-RRB-	denotes	)
T25235	23515-23523	NN	denotes	HA-IκB-α
T25236	23524-23525	-LRB-	denotes	(
T25237	23525-23529	NN	denotes	5 µl
T25238	23529-23530	-RRB-	denotes	)
T25239	23531-23534	VB	denotes	was
T25240	23535-23544	VB	denotes	incubated
T25241	23545-23547	IN	denotes	in
T25242	23548-23556	NN	denotes	presence
T25243	23557-23559	CC	denotes	or
T25244	23560-23567	NN	denotes	absence
T25245	23568-23570	IN	denotes	of
T25246	23571-23579	NN	denotes	FLAG-Tat
T25247	23579-23580	-COMMA-	denotes	,
T25248	23581-23589	NN	denotes	FLAG-Tat
T25249	23590-23591	NNP	denotes	T
T25250	23591-23592	-COMMA-	denotes	,
T25251	23592-23593	NN	denotes	N
T25252	23593-23594	-LRB-	denotes	(
T25253	23594-23599	CD	denotes	23,24
T25254	23599-23600	-RRB-	denotes	)
T25255	23600-23601	NN	denotes	A
T25256	23601-23602	-COMMA-	denotes	,
T25257	23603-23611	NN	denotes	FLAG-Tat
T25258	23612-23613	NN	denotes	K
T25259	23613-23614	-LRB-	denotes	(
T25260	23614-23619	CD	denotes	50,51
T25261	23619-23620	-RRB-	denotes	)
T25262	23620-23621	NN	denotes	A
T25263	23621-23622	-COMMA-	denotes	,
T25264	23623-23631	NN	denotes	FLAG-Tat
T25265	23632-23633	NN	denotes	R
T25266	23633-23634	-LRB-	denotes	(
T25267	23634-23639	CD	denotes	49–57
T25268	23639-23640	-RRB-	denotes	)
T25269	23640-23641	NN	denotes	A
T25270	23642-23644	CC	denotes	or
T25271	23645-23653	NN	denotes	FLAG-Tat
T25272	23654-23655	NN	denotes	C
T25273	23655-23656	-LRB-	denotes	(
T25274	23656-23664	CD	denotes	22,25,27
T25275	23664-23665	-RRB-	denotes	)
T25276	23665-23666	NN	denotes	A
T25277	23667-23668	-LRB-	denotes	(
T25278	23668-23673	NN	denotes	10 µl
T25279	23673-23674	-RRB-	denotes	)
T25280	23675-23680	VB	denotes	using
T25281	23681-23683	FW	denotes	in
T25282	23684-23689	FW	denotes	vitro
T25283	23690-23700	VB	denotes	translated
T25284	23701-23709	NN	denotes	proteins
T25285	23711-23720	JJ	denotes	Wild-type
T25286	23721-23724	CC	denotes	and
T25287	23725-23731	JJ	denotes	mutant
T25288	23732-23735	NN	denotes	Tat
T25289	23736-23744	NN	denotes	proteins
T25290	23745-23749	VB	denotes	were
T25291	23750-23768	VB	denotes	immunoprecipitated
T25292	23769-23773	IN	denotes	with
T25293	23774-23783	JJ	denotes	anti-FLAG
T25294	23784-23792	NN	denotes	antibody
T25295	23792-23793	-COLON-	denotes	;
T25296	23794-23809	NN	denotes	immunocomplexes
T25297	23810-23814	VB	denotes	were
T25298	23815-23824	VB	denotes	separated
T25299	23825-23827	IN	denotes	by
T25300	23828-23830	CD	denotes	12
T25301	23830-23831	NN	denotes	%
T25302	23832-23840	NN	denotes	SDS–PAGE
T25303	23841-23844	CC	denotes	and
T25304	23845-23853	VB	denotes	analysed
T25305	23854-23856	IN	denotes	by
T25306	23857-23864	JJ	denotes	western
T25307	23865-23873	NN	denotes	blotting
T25308	23874-23878	IN	denotes	with
T25309	23879-23886	JJ	denotes	anti-HA
T25310	23887-23890	CC	denotes	and
T25311	23891-23900	JJ	denotes	anti-FLAG
T25312	23901-23912	NN	denotes	antibodies.
T25313	23913-23914	-LRB-	denotes	(
T25314	23914-23915	NN	denotes	C
T25315	23915-23916	-RRB-	denotes	)
T25316	23917-23939	JJ	denotes	35S-methionine-labeled
T25317	23940-23943	NN	denotes	p65
T25318	23944-23945	-LRB-	denotes	(
T25319	23945-23949	NN	denotes	5 µl
T25320	23949-23950	-RRB-	denotes	)
T25321	23951-23954	VB	denotes	was
T25322	23955-23964	VB	denotes	incubated
T25323	23965-23969	IN	denotes	with
T25324	23970-23972	FW	denotes	in
T25325	23973-23978	FW	denotes	vitro
T25326	23979-23989	VB	denotes	translated
T25327	23990-23998	NN	denotes	HA-IκB-α
T25328	23999-24000	-LRB-	denotes	(
T25329	24000-24004	NN	denotes	5 µl
T25330	24004-24005	-RRB-	denotes	)
T25331	24006-24008	IN	denotes	in
T25332	24009-24017	NN	denotes	presence
T25333	24018-24020	CC	denotes	or
T25334	24021-24028	NN	denotes	absence
T25335	24029-24031	IN	denotes	of
T25336	24032-24040	NN	denotes	FLAG-Tat
T25337	24041-24043	CC	denotes	or
T25338	24044-24052	NN	denotes	FLAG-Tat
T25339	24053-24054	NN	denotes	R
T25340	24054-24055	-LRB-	denotes	(
T25341	24055-24060	CD	denotes	49–57
T25342	24060-24061	-RRB-	denotes	)
T25343	24061-24062	NN	denotes	A
T25344	24063-24064	-LRB-	denotes	(
T25345	24064-24065	CD	denotes	5
T25346	24065-24066	-COMMA-	denotes	,
T25347	24067-24069	CD	denotes	10
T25348	24070-24072	CC	denotes	or
T25349	24073-24078	NN	denotes	20 µl
T25350	24078-24079	-RRB-	denotes	)
T25351	24081-24089	NN	denotes	HA-IκB-α
T25352	24090-24093	VB	denotes	was
T25353	24094-24112	VB	denotes	immunoprecipitated
T25354	24113-24117	IN	denotes	with
T25355	24118-24125	JJ	denotes	anti-HA
T25356	24126-24134	NN	denotes	antibody
T25357	24134-24135	-COLON-	denotes	;
T25358	24136-24151	NN	denotes	immunocomplexes
T25359	24152-24156	VB	denotes	were
T25360	24157-24166	VB	denotes	separated
T25361	24167-24169	IN	denotes	by
T25362	24170-24172	CD	denotes	12
T25363	24172-24173	NN	denotes	%
T25364	24174-24182	NN	denotes	SDS–PAGE
T25365	24183-24186	CC	denotes	and
T25366	24187-24195	VB	denotes	analysed
T25367	24196-24198	IN	denotes	by
T25368	24199-24206	JJ	denotes	western
T25369	24207-24215	NN	denotes	blotting
T25370	24216-24220	IN	denotes	with
T25371	24221-24228	JJ	denotes	anti-HA
T25372	24229-24232	CC	denotes	and
T25373	24233-24242	JJ	denotes	anti-FLAG
T25374	24243-24253	NN	denotes	antibodies
T25375	24253-24254	-COMMA-	denotes	,
T25376	24255-24257	CC	denotes	or
T25377	24258-24273	NN	denotes	autoradiography
T25378	24274-24275	-LRB-	denotes	(
T25379	24275-24286	NN	denotes	35S-Met-p65
T25380	24286-24287	-RRB-	denotes	)
T25381	24289-24301	NN	denotes	Densitometry
T25382	24302-24308	NN	denotes	values
T25383	24309-24310	-LRB-	denotes	(
T25384	24310-24311	NN	denotes	D
T25385	24311-24312	-RRB-	denotes	)
T25386	24313-24315	IN	denotes	of
T25387	24316-24319	DT	denotes	the
T25388	24320-24325	NN	denotes	bands
T25389	24326-24330	VB	denotes	were
T25390	24331-24340	VB	denotes	expressed
T25391	24341-24343	IN	denotes	as
T25392	24344-24348	JJ	denotes	fold
T25393	24349-24357	NN	denotes	increase
T25394	24358-24363	IN	denotes	above
T25395	24364-24367	DT	denotes	the
T25396	24368-24375	NN	denotes	control
T25397	24376-24377	-LRB-	denotes	(
T25398	24377-24381	NN	denotes	lane
T25399	24382-24383	CD	denotes	1
T25400	24383-24384	-RRB-	denotes	)
T25401	24384-24385	-LRB-	denotes	.
T25402	24386-24387	NN	denotes	(
T25403	24387-24388	-RRB-	denotes	D
T25404	24388-24391	DT	denotes	) T
T25405	24391-24394	NN	denotes	he
T25406	24394-24397	NN	denotes	p65
T25407	24398-24405	NN	denotes	DNA bin
T25408	24405-24413	NN	denotes	ding act
T25409	24413-24416	VB	denotes	ivi
T25410	24416-24424	VB	denotes	ty was a
T25411	24424-24426	IN	denotes	na
T25412	24426-24430	NN	denotes	lyse
T25413	24430-24435	VB	denotes	d by
T25414	24435-24437	FW	denotes	EM
T25415	24437-24442	FW	denotes	SA us
T25416	24442-24452	VB	denotes	ing in vit
T25417	24452-24460	NN	denotes	ro trans
T25418	24476-24493	NN	denotes	32P-labeled-NF-κB
T25419	24494-24509	JJ	denotes	double-stranded
T25420	24510-24525	NN	denotes	oligonucleotide
T25421	24526-24529	VB	denotes	was
T25422	24530-24539	VB	denotes	incubated
T25423	24540-24544	IN	denotes	with
T25424	24545-24548	NN	denotes	p65
T25425	24549-24550	-LRB-	denotes	(
T25426	24550-24556	NN	denotes	0.5 µl
T25427	24556-24557	-RRB-	denotes	)
T25428	24558-24560	IN	denotes	in
T25429	24561-24569	NN	denotes	presence
T25430	24570-24572	CC	denotes	or
T25431	24573-24580	NN	denotes	absence
T25432	24581-24583	IN	denotes	of
T25433	24584-24592	NN	denotes	HA-IκB-α
T25434	24593-24594	-LRB-	denotes	(
T25435	24594-24598	NN	denotes	1 µl
T25436	24598-24599	-RRB-	denotes	)
T25437	24599-24600	-COMMA-	denotes	,
T25438	24601-24609	NN	denotes	FLAG-Tat
T25439	24610-24612	CC	denotes	or
T25440	24613-24621	NN	denotes	FLAG-Tat
T25441	24622-24623	NN	denotes	R
T25442	24623-24624	-LRB-	denotes	(
T25443	24624-24629	CD	denotes	49–57
T25444	24629-24630	-RRB-	denotes	)
T25445	24630-24631	NN	denotes	A
T25446	24632-24633	-LRB-	denotes	(
T25447	24633-24634	CD	denotes	5
T25448	24635-24638	CC	denotes	and
T25449	24639-24644	NN	denotes	10 µl
T25450	24644-24645	-RRB-	denotes	)
T25451	24645-24646	-COLON-	denotes	;
T25452	24647-24658	NN	denotes	competition
T25453	24659-24661	IN	denotes	of
T25454	24662-24665	NN	denotes	DNA
T25455	24666-24673	NN	denotes	binding
T25456	24674-24677	VB	denotes	was
T25457	24678-24687	VB	denotes	performed
T25458	24688-24692	IN	denotes	with
T25459	24693-24697	CD	denotes	10 -
T25460	24698-24700	IN	denotes	up
T25461	24701-24703	TO	denotes	to
T25462	24704-24712	JJ	denotes	100-fold
T25463	24713-24718	JJ	denotes	molar
T25464	24719-24725	NN	denotes	excess
T25465	24726-24728	IN	denotes	of
T25466	24729-24738	JJ	denotes	unlabeled
T25467	24739-24744	NN	denotes	NF-κB
T25468	24745-24760	JJ	denotes	double-stranded
T25469	24761-24776	NN	denotes	oligonucleotide
T25470	24778-24789	NN	denotes	DNA/protein
T25471	24790-24799	NN	denotes	complexes
T25472	24800-24804	VB	denotes	were
T25473	24805-24808	VB	denotes	run
T25474	24809-24811	IN	denotes	on
T25475	24812-24813	CD	denotes	6
T25476	24813-24814	NN	denotes	%
T25477	24815-24823	NN	denotes	PAGE–TBE
T25478	24824-24827	CC	denotes	and
T25479	24828-24836	VB	denotes	analysed
T25480	24837-24839	IN	denotes	by
T25481	24840-24856	NN	denotes	autoradiography.
T25482	24857-24858	-LRB-	denotes	(
T25483	24858-24859	NN	denotes	E
T25484	24859-24860	-RRB-	denotes	)
T25485	24861-24877	NN	denotes	p50−/−p65−/−MEFs
T25486	24878-24879	-LRB-	denotes	(
T25487	24879-24886	CD	denotes	3 × 105
T25488	24887-24892	NN	denotes	cells
T25489	24892-24893	-RRB-	denotes	)
T25490	24894-24898	VB	denotes	were
T25491	24899-24910	VB	denotes	transfected
T25492	24911-24915	IN	denotes	with
T25493	24916-24922	NN	denotes	pκBLuc
T25494	24923-24924	-LRB-	denotes	(
T25495	24924-24930	NN	denotes	0.5 µg
T25496	24930-24931	-RRB-	denotes	)
T25497	24932-24935	CC	denotes	and
T25498	24936-24945	JJ	denotes	pSV-β-Gal
T25499	24946-24947	-LRB-	denotes	(
T25500	24947-24953	CD	denotes	0.1 µg
T25501	24953-24954	-RRB-	denotes	)
T25502	24955-24959	IN	denotes	with
T25503	24960-24962	CC	denotes	or
T25504	24963-24970	IN	denotes	without
T25505	24971-24982	NN	denotes	pRc/CMV-p65
T25506	24983-24984	-LRB-	denotes	(
T25507	24984-24990	CD	denotes	0.5 µg
T25508	24990-24991	-RRB-	denotes	)
T25509	24991-24992	-COMMA-	denotes	,
T25510	24993-25007	NN	denotes	pCMV4-HA-IκB-α
T25511	25008-25009	-LRB-	denotes	(
T25512	25009-25015	CD	denotes	0.5 µg
T25513	25015-25016	-RRB-	denotes	)
T25514	25016-25017	-COMMA-	denotes	,
T25515	25018-25029	NN	denotes	p3xFLAG-Tat
T25516	25030-25032	CC	denotes	or
T25517	25033-25044	NN	denotes	p3xFLAG-Tat
T25518	25045-25046	NN	denotes	R
T25519	25046-25047	-LRB-	denotes	(
T25520	25047-25052	CD	denotes	49–57
T25521	25052-25053	-RRB-	denotes	)
T25522	25053-25054	NN	denotes	A
T25523	25055-25056	-LRB-	denotes	(
T25524	25056-25059	CD	denotes	0.5
T25525	25059-25060	-COMMA-	denotes	,
T25526	25061-25062	CD	denotes	1
T25527	25063-25066	CC	denotes	and
T25528	25067-25071	NN	denotes	2 µg
T25529	25071-25072	-RRB-	denotes	)
T25530	25074-25077	DT	denotes	The
T25531	25078-25088	NN	denotes	luciferase
T25532	25089-25097	NN	denotes	activity
T25533	25098-25101	VB	denotes	was
T25534	25102-25110	VB	denotes	measured
T25535	25111-25113	IN	denotes	in
T25536	25114-25118	NN	denotes	cell
T25537	25119-25127	NN	denotes	extracts
T25538	25128-25132	JJ	denotes	48-h
T25539	25133-25150	JJ	denotes	post-transfection
T25540	25151-25154	CC	denotes	and
T25541	25155-25165	VB	denotes	normalized
T25542	25166-25168	TO	denotes	to
T25543	25169-25184	JJ	denotes	β-galactosidase
T25544	25185-25193	NN	denotes	activity
T25545	25195-25199	JJ	denotes	Fold
T25546	25200-25210	NN	denotes	activation
T25547	25211-25214	VB	denotes	was
T25548	25215-25225	VB	denotes	calculated
T25549	25226-25234	JJ	denotes	relative
T25550	25235-25237	TO	denotes	to
T25551	25238-25250	NN	denotes	transfection
T25552	25251-25253	IN	denotes	of
T25553	25254-25257	DT	denotes	the
T25554	25258-25264	NN	denotes	pκBLuc
T25555	25265-25272	NN	denotes	plasmid
T25556	25273-25278	RB	denotes	alone
T25557	25280-25286	NN	denotes	Values
T25558	25287-25288	-LRB-	denotes	(
T25559	25288-25297	NN	denotes	mean ± SE
T25560	25297-25298	-COMMA-	denotes	,
T25561	25299-25304	NN	denotes	n = 3
T25562	25304-25305	-RRB-	denotes	)
T25563	25306-25309	VB	denotes	are
T25564	25310-25315	VB	denotes	shown
T25565	25317-25319	IN	denotes	As
T25566	25320-25327	NN	denotes	control
T25567	25328-25330	IN	denotes	of
T25568	25331-25338	NN	denotes	protein
T25569	25339-25349	NN	denotes	expression
T25570	25349-25350	-COMMA-	denotes	,
T25571	25351-25359	NN	denotes	aliquots
T25572	25360-25362	IN	denotes	of
T25573	25363-25367	NN	denotes	cell
T25574	25368-25376	NN	denotes	extracts
T25575	25377-25378	-LRB-	denotes	(
T25576	25378-25383	NN	denotes	20 µg
T25577	25383-25384	-RRB-	denotes	)
T25578	25385-25389	VB	denotes	were
T25579	25390-25398	VB	denotes	analysed
T25580	25399-25401	IN	denotes	by
T25581	25402-25409	JJ	denotes	western
T25582	25410-25418	NN	denotes	blotting
T25583	25419-25423	IN	denotes	with
T25584	25424-25432	JJ	denotes	anti-p65
T25585	25432-25433	-COMMA-	denotes	,
T25586	25434-25441	JJ	denotes	anti-HA
T25587	25441-25442	-COMMA-	denotes	,
T25588	25443-25452	JJ	denotes	anti-FLAG
T25589	25453-25456	CC	denotes	and
T25590	25457-25471	JJ	denotes	anti-γ-tubulin
T25591	25472-25482	NN	denotes	antibodies
T27266	31394-31397	NN	denotes	Tat
T27267	31398-31407	VB	denotes	activates
T27268	31408-31411	DT	denotes	the
T27269	31412-31425	JJ	denotes	p65-dependent
T27270	31426-31436	NN	denotes	expression
T27271	31437-31439	IN	denotes	of
T27272	31440-31456	JJ	denotes	NF-κB-responsive
T27273	31457-31462	NN	denotes	genes
T27274	31463-31465	IN	denotes	by
T27275	31466-31475	VB	denotes	occupying
T27276	31476-31479	DT	denotes	the
T27277	31480-31485	NN	denotes	NF-κB
T27278	31486-31495	NN	denotes	enhancers
T27279	31496-31499	CC	denotes	and
T27280	31500-31509	VB	denotes	promoting
T27281	31510-31513	DT	denotes	the
T27282	31514-31517	NN	denotes	p65
T27283	31518-31529	NN	denotes	recruitment
T27284	31530-31534	IN	denotes	with
T27285	31535-31540	JJ	denotes	IκB-α
T27286	31541-31553	NN	denotes	displacement
T27287	31555-31559	NN	denotes	HeLa
T27288	31560-31565	NN	denotes	cells
T27289	31566-31567	-LRB-	denotes	(
T27290	31567-31574	CD	denotes	5 × 106
T27291	31574-31575	-RRB-	denotes	)
T27292	31576-31580	VB	denotes	were
T27293	31581-31592	VB	denotes	transfected
T27294	31593-31597	IN	denotes	with
T27295	31598-31609	NN	denotes	p3xFLAG-Tat
T27296	31609-31610	-COMMA-	denotes	,
T27297	31611-31622	NN	denotes	p3xFLAG-Tat
T27298	31623-31624	NN	denotes	C
T27299	31624-31625	-LRB-	denotes	(
T27300	31625-31633	CD	denotes	22,25,27
T27301	31633-31634	-RRB-	denotes	)
T27302	31634-31635	-COMMA-	denotes	,
T27303	31636-31647	NN	denotes	p3xFLAG-Tat
T27304	31648-31649	NN	denotes	R
T27305	31649-31650	-LRB-	denotes	(
T27306	31650-31655	CD	denotes	49–57
T27307	31655-31656	-RRB-	denotes	)
T27308	31657-31659	CC	denotes	or
T27309	31660-31665	JJ	denotes	empty
T27310	31666-31672	NN	denotes	vector
T27311	31673-31674	-LRB-	denotes	(
T27312	31674-31679	NN	denotes	10 µg
T27313	31679-31680	-RRB-	denotes	)
T27314	31680-31681	-COLON-	denotes	;
T27315	31682-31685	IN	denotes	for
T27316	31686-31689	NN	denotes	p65
T27317	31690-31693	NN	denotes	RNA
T27318	31694-31706	NN	denotes	interference
T27319	31706-31707	-COMMA-	denotes	,
T27320	31708-31713	NN	denotes	cells
T27321	31714-31715	-LRB-	denotes	(
T27322	31715-31722	CD	denotes	5 × 106
T27323	31722-31723	-RRB-	denotes	)
T27324	31724-31728	VB	denotes	were
T27325	31729-31740	VB	denotes	transfected
T27326	31741-31745	IN	denotes	with
T27327	31746-31757	NN	denotes	p3xFLAG-Tat
T27328	31758-31759	-LRB-	denotes	(
T27329	31759-31764	NN	denotes	10 µg
T27330	31764-31765	-RRB-	denotes	)
T27331	31766-31769	CC	denotes	and
T27332	31770-31775	NN	denotes	siRNA
T27333	31776-31779	NN	denotes	p65
T27334	31780-31782	CC	denotes	or
T27335	31783-31788	NN	denotes	siRNA
T27336	31789-31796	NN	denotes	control
T27337	31797-31798	-LRB-	denotes	(
T27338	31798-31806	NN	denotes	200 pmol
T27339	31806-31807	-RRB-	denotes	)
T27340	31809-31825	CD	denotes	Twenty-four-hour
T27341	31826-31843	JJ	denotes	post-transfection
T27342	31843-31844	-COMMA-	denotes	,
T27343	31845-31850	JJ	denotes	total
T27344	31851-31854	NN	denotes	RNA
T27345	31855-31858	VB	denotes	was
T27346	31859-31868	VB	denotes	extracted
T27347	31869-31872	CC	denotes	and
T27348	31873-31881	VB	denotes	analysed
T27349	31882-31884	IN	denotes	by
T27350	31885-31894	JJ	denotes	real-time
T27351	31895-31898	NN	denotes	PCR
T27352	31899-31901	TO	denotes	to
T27353	31902-31910	VB	denotes	evaluate
T27354	31911-31914	DT	denotes	the
T27355	31915-31925	NN	denotes	expression
T27356	31926-31928	IN	denotes	of
T27357	31929-31935	NN	denotes	MIP-1α
T27358	31936-31937	-LRB-	denotes	(
T27359	31937-31938	NN	denotes	A
T27360	31938-31939	-RRB-	denotes	)
T27361	31939-31940	-COMMA-	denotes	,
T27362	31941-31945	NN	denotes	CSF3
T27363	31946-31947	-LRB-	denotes	(
T27364	31947-31948	NN	denotes	B
T27365	31948-31949	-RRB-	denotes	)
T27366	31949-31950	-COMMA-	denotes	,
T27367	31951-31954	NN	denotes	LTA
T27368	31955-31956	-LRB-	denotes	(
T27369	31956-31957	NN	denotes	C
T27370	31957-31958	-RRB-	denotes	)
T27371	31958-31959	-COMMA-	denotes	,
T27372	31960-31966	NN	denotes	NFKBIA
T27373	31967-31968	-LRB-	denotes	(
T27374	31968-31969	NN	denotes	D
T27375	31969-31970	-RRB-	denotes	)
T27376	31970-31971	-COMMA-	denotes	,
T27377	31972-31976	NN	denotes	TLR2
T27378	31977-31978	-LRB-	denotes	(
T27379	31978-31979	NN	denotes	E
T27380	31979-31980	-RRB-	denotes	)
T27381	31980-31981	-COMMA-	denotes	,
T27382	31982-31987	NN	denotes	GAPDH
T27383	31988-31989	-LRB-	denotes	(
T27384	31989-31990	NN	denotes	F
T27385	31990-31991	-RRB-	denotes	)
T27386	31991-31992	-COMMA-	denotes	,
T27387	31993-31997	NN	denotes	ACTB
T27388	31998-31999	-LRB-	denotes	(
T27389	31999-32000	NN	denotes	G
T27390	32000-32001	-RRB-	denotes	)
T27391	32002-32008	NN	denotes	genes.
T27392	32009-32010	-LRB-	denotes	(
T27393	32010-32011	NN	denotes	H
T27394	32011-32012	-RRB-	denotes	)
T27395	32013-32017	NN	denotes	HeLa
T27396	32018-32023	NN	denotes	cells
T27397	32024-32025	-LRB-	denotes	(
T27398	32025-32032	CD	denotes	5 × 106
T27399	32032-32033	-RRB-	denotes	)
T27400	32034-32038	VB	denotes	were
T27401	32039-32050	VB	denotes	transfected
T27402	32051-32055	IN	denotes	with
T27403	32056-32067	NN	denotes	p3xFLAG-Tat
T27404	32067-32068	-COMMA-	denotes	,
T27405	32069-32071	CC	denotes	or
T27406	32072-32077	JJ	denotes	empty
T27407	32078-32084	NN	denotes	vector
T27408	32085-32086	-LRB-	denotes	(
T27409	32086-32091	NN	denotes	10 µg
T27410	32091-32092	-RRB-	denotes	)
T27411	32093-32096	CC	denotes	and
T27412	32097-32101	NN	denotes	48 h
T27413	32102-32107	JJ	denotes	later
T27414	32108-32112	NN	denotes	ChIP
T27415	32113-32116	VB	denotes	was
T27416	32117-32126	VB	denotes	performed
T27417	32127-32131	IN	denotes	with
T27418	32132-32140	JJ	denotes	anti-p65
T27419	32141-32149	NN	denotes	antibody
T27420	32151-32160	JJ	denotes	Real-time
T27421	32161-32164	NN	denotes	PCR
T27422	32165-32168	VB	denotes	was
T27423	32169-32178	VB	denotes	performed
T27424	32179-32183	IN	denotes	with
T27425	32184-32191	NN	denotes	primers
T27426	32192-32200	JJ	denotes	specific
T27427	32201-32204	IN	denotes	for
T27428	32205-32208	DT	denotes	the
T27429	32209-32218	VB	denotes	indicated
T27430	32219-32229	NN	denotes	promoters.
T27431	32230-32231	-LRB-	denotes	(
T27432	32231-32232	CD	denotes	I
T27433	32232-32233	-RRB-	denotes	)
T27434	32234-32238	NN	denotes	HeLa
T27435	32239-32244	NN	denotes	cells
T27436	32245-32246	-LRB-	denotes	(
T27437	32246-32253	CD	denotes	5 × 106
T27438	32253-32254	-RRB-	denotes	)
T27439	32255-32259	VB	denotes	were
T27440	32260-32271	VB	denotes	transfected
T27441	32272-32276	IN	denotes	with
T27442	32277-32282	JJ	denotes	empty
T27443	32283-32289	NN	denotes	vector
T27444	32289-32290	-COMMA-	denotes	,
T27445	32291-32293	CC	denotes	or
T27446	32294-32305	NN	denotes	p3xFLAG-Tat
T27447	32306-32307	-LRB-	denotes	(
T27448	32307-32312	NN	denotes	10 µg
T27449	32312-32313	-RRB-	denotes	)
T27450	32314-32316	IN	denotes	in
T27451	32317-32325	NN	denotes	presence
T27452	32326-32328	CC	denotes	or
T27453	32329-32336	NN	denotes	absence
T27454	32337-32339	IN	denotes	of
T27455	32340-32345	NN	denotes	siRNA
T27456	32346-32349	NN	denotes	p65
T27457	32349-32350	-COMMA-	denotes	,
T27458	32351-32353	CC	denotes	or
T27459	32354-32359	NN	denotes	siRNA
T27460	32360-32367	NN	denotes	control
T27461	32368-32369	-LRB-	denotes	(
T27462	32369-32377	NN	denotes	200 pmol
T27463	32377-32378	-RRB-	denotes	)
T27464	32378-32379	-COLON-	denotes	;
T27465	32380-32384	NN	denotes	48 h
T27466	32385-32390	RB	denotes	later
T27467	32390-32391	-COMMA-	denotes	,
T27468	32392-32396	NN	denotes	ChIP
T27469	32397-32400	VB	denotes	was
T27470	32401-32410	VB	denotes	performed
T27471	32411-32415	IN	denotes	with
T27472	32416-32425	JJ	denotes	anti-FLAG
T27473	32426-32434	NN	denotes	antibody
T27474	32436-32445	JJ	denotes	Real-time
T27475	32446-32449	NN	denotes	PCR
T27476	32450-32453	VB	denotes	was
T27477	32454-32463	VB	denotes	performed
T27478	32464-32468	IN	denotes	with
T27479	32469-32476	NN	denotes	primers
T27480	32477-32485	JJ	denotes	specific
T27481	32486-32489	IN	denotes	for
T27482	32490-32493	DT	denotes	the
T27483	32494-32503	VB	denotes	indicated
T27484	32504-32514	NN	denotes	promoters.
T27485	32515-32516	-LRB-	denotes	(
T27486	32516-32517	NN	denotes	J
T27487	32517-32518	-RRB-	denotes	)
T27488	32519-32523	NN	denotes	HeLa
T27489	32524-32529	NN	denotes	cells
T27490	32530-32531	-LRB-	denotes	(
T27491	32531-32538	CD	denotes	5 × 106
T27492	32538-32539	-RRB-	denotes	)
T27493	32540-32544	VB	denotes	were
T27494	32545-32556	VB	denotes	transfected
T27495	32557-32561	IN	denotes	with
T27496	32562-32567	JJ	denotes	empty
T27497	32568-32574	NN	denotes	vector
T27498	32574-32575	-COMMA-	denotes	,
T27499	32576-32587	NN	denotes	p3xFLAG-Tat
T27500	32588-32589	-LRB-	denotes	(
T27501	32589-32594	NN	denotes	10 µg
T27502	32594-32595	-RRB-	denotes	)
T27503	32595-32596	-COMMA-	denotes	,
T27504	32597-32599	CC	denotes	or
T27505	32600-32605	JJ	denotes	empty
T27506	32606-32612	NN	denotes	vector
T27507	32613-32617	CC	denotes	plus
T27508	32618-32623	NN	denotes	siRNA
T27509	32624-32629	NN	denotes	IκB-α
T27510	32630-32632	CC	denotes	or
T27511	32633-32638	NN	denotes	siRNA
T27512	32639-32646	NN	denotes	control
T27513	32647-32648	-LRB-	denotes	(
T27514	32648-32656	NN	denotes	200 pmol
T27515	32656-32657	-RRB-	denotes	)
T27516	32657-32658	-COLON-	denotes	;
T27517	32659-32663	NN	denotes	48 h
T27518	32664-32669	RB	denotes	later
T27519	32669-32670	-COMMA-	denotes	,
T27520	32671-32675	NN	denotes	ChIP
T27521	32676-32679	VB	denotes	was
T27522	32680-32689	VB	denotes	performed
T27523	32690-32694	IN	denotes	with
T27524	32695-32705	JJ	denotes	anti-IκB-α
T27525	32706-32714	NN	denotes	antibody
T27526	32716-32725	JJ	denotes	Real-time
T27527	32726-32729	NN	denotes	PCR
T27528	32730-32733	VB	denotes	was
T27529	32734-32743	VB	denotes	performed
T27530	32744-32748	IN	denotes	with
T27531	32749-32756	NN	denotes	primers
T27532	32757-32765	JJ	denotes	specific
T27533	32766-32769	IN	denotes	for
T27534	32770-32773	DT	denotes	the
T27535	32774-32783	JJ	denotes	indicated
T27536	32784-32793	NN	denotes	promoters
T27537	32795-32801	NN	denotes	Values
T27538	32802-32803	-LRB-	denotes	(
T27539	32803-32812	NN	denotes	mean ± SE
T27540	32812-32813	-COMMA-	denotes	,
T27541	32814-32819	NN	denotes	n = 3
T27542	32819-32820	-RRB-	denotes	)
T27543	32821-32824	VB	denotes	are
T27544	32825-32830	VB	denotes	shown
T27545	32832-32835	DT	denotes	The
T27546	32836-32845	NN	denotes	asterisks
T27547	32846-32854	VB	denotes	indicate
T27548	32855-32868	RB	denotes	statistically
T27549	32869-32880	JJ	denotes	significant
T27550	32881-32892	NN	denotes	differences
T27551	32893-32901	VB	denotes	compared
T27552	32902-32904	TO	denotes	to
T27553	32905-32908	DT	denotes	the
T27554	32909-32916	NN	denotes	control
T27555	32917-32918	-LRB-	denotes	(
T27556	32918-32923	JJ	denotes	empty
T27557	32924-32930	NN	denotes	vector
T27558	32930-32931	-RRB-	denotes	)
T27559	32932-32941	VB	denotes	according
T27560	32942-32944	TO	denotes	to
T27561	32945-32948	DT	denotes	the
T27562	32949-32956	NN	denotes	Student
T27563	32956-32958	POS	denotes	's
T27564	32959-32965	NN	denotes	t-test
T27565	32966-32967	-LRB-	denotes	(
T27566	32967-32969	NN	denotes	P
T27567	32969-32970	SYM	denotes	<
T27568	32970-32975	CD	denotes	0.05
T27569	32975-32976	-RRB-	denotes	)
T29104	43632-43637	NN	denotes	Model
T29105	43638-43640	IN	denotes	of
T29106	43641-43647	NN	denotes	MIP-1α
T29107	43648-43663	JJ	denotes	transcriptional
T29108	43664-43674	NN	denotes	activation
T29109	43675-43677	IN	denotes	by
T29110	43678-43683	NN	denotes	HIV-1
T29111	43684-43688	NN	denotes	Tat.
T29112	43689-43690	-LRB-	denotes	(
T29113	43690-43691	NN	denotes	A
T29114	43691-43692	-RRB-	denotes	)
T29115	43693-43695	IN	denotes	In
T29116	43696-43703	NN	denotes	absence
T29117	43704-43706	IN	denotes	of
T29118	43707-43710	NN	denotes	Tat
T29119	43710-43711	-COMMA-	denotes	,
T29120	43712-43717	NNP	denotes	IκB-α
T29121	43718-43726	VB	denotes	occupies
T29122	43727-43730	DT	denotes	the
T29123	43731-43736	NN	denotes	NF-κB
T29124	43737-43745	NN	denotes	enhancer
T29125	43746-43748	IN	denotes	of
T29126	43749-43752	DT	denotes	the
T29127	43753-43759	NN	denotes	MIP-1α
T29128	43760-43768	NN	denotes	promoter
T29129	43769-43772	CC	denotes	and
T29130	43773-43779	RB	denotes	likely
T29131	43780-43789	VB	denotes	interacts
T29132	43790-43794	IN	denotes	with
T29133	43795-43810	JJ	denotes	transcriptional
T29134	43811-43821	NN	denotes	repressors
T29135	43821-43822	-COMMA-	denotes	,
T29136	43823-43827	JJ	denotes	such
T29137	43828-43830	IN	denotes	as
T29138	43831-43836	NN	denotes	HDACs
T29139	43837-43838	-LRB-	denotes	(
T29140	43838-43840	CD	denotes	65
T29141	43840-43841	-RRB-	denotes	)
T29142	43841-43842	-COMMA-	denotes	,
T29143	43843-43845	TO	denotes	to
T29144	43846-43853	VB	denotes	inhibit
T29145	43854-43858	NN	denotes	gene
T29146	43859-43873	NN	denotes	transcription.
T29147	43874-43875	-LRB-	denotes	(
T29148	43875-43876	NN	denotes	B
T29149	43876-43877	-RRB-	denotes	)
T29150	43878-43881	NN	denotes	Tat
T29151	43882-43891	VB	denotes	activates
T29152	43892-43895	DT	denotes	the
T29153	43896-43902	NN	denotes	MIP-1α
T29154	43903-43913	NN	denotes	expression
T29155	43914-43916	IN	denotes	by
T29156	43917-43925	VB	denotes	removing
T29157	43926-43929	DT	denotes	the
T29158	43930-43935	JJ	denotes	IκB-α
T29159	43936-43945	NN	denotes	repressor
T29160	43946-43950	IN	denotes	from
T29161	43951-43954	DT	denotes	the
T29162	43955-43960	NN	denotes	NF-κB
T29163	43961-43969	NN	denotes	enhancer
T29164	43969-43970	-COMMA-	denotes	,
T29165	43971-43974	CC	denotes	and
T29166	43975-43977	IN	denotes	by
T29167	43978-43988	VB	denotes	increasing
T29168	43989-43992	DT	denotes	the
T29169	43993-44000	NN	denotes	binding
T29170	44001-44003	IN	denotes	of
T29171	44004-44007	NN	denotes	p65
T29172	44008-44013	NN	denotes	NF-κB
T29173	44014-44021	NN	denotes	complex
T29174	44022-44024	TO	denotes	to
T29175	44025-44028	DT	denotes	the
T29176	44029-44034	NN	denotes	NF-κB
T29177	44035-44043	NN	denotes	enhancer
T6077	9014-9017	CC	denotes	and
T6895	11282-11285	NN	denotes	GST
T6896	11286-11294	NN	denotes	proteins
T6897	11295-11297	IN	denotes	in
T6898	11298-11309	FW	denotes	Escherichia
T6899	11310-11314	FW	denotes	coli
T6900	11315-11321	NN	denotes	strain
T6901	11322-11326	NN	denotes	BL21
T6902	11327-11331	VB	denotes	were
T6903	11332-11341	VB	denotes	performed
T6904	11342-11344	IN	denotes	as
T6905	11345-11355	RB	denotes	previously
T6906	11356-11364	VB	denotes	reported
T6907	11365-11366	-LRB-	denotes	(
T6908	11366-11368	CD	denotes	50
T6909	11368-11369	-RRB-	denotes	)
T6910	11371-11378	NN	denotes	Details
T6911	11379-11382	VB	denotes	are
T6912	11383-11392	VB	denotes	described
T6913	11393-11395	IN	denotes	in
T6914	11396-11409	NNP	denotes	Supplementary
T6915	11410-11414	NNP	denotes	Data
R74	T145	T142	arg1Of	Tat,Human
R75	T145	T143	arg1Of	Tat,immunodeficiency
R76	T145	T144	arg1Of	Tat,virus-1
R77	T145	T146	arg1Of	Tat,activates
R78	T146	T148	arg1Of	activates,via
R79	T147	T146	arg2Of	NF-κB,activates
R80	T150	T148	arg2Of	interaction,via
R81	T150	T149	arg1Of	interaction,physical
R82	T150	T151	arg1Of	interaction,with
R83	T152	T153	arg1Of	IκB-α,and
R84	T153	T151	arg2Of	and,with
R85	T154	T153	arg2Of	p65,and
R86	T156	T155	arg1Of	factor,Nuclear
R87	T156	T157	arg1Of	factor,(
R88	T158	T157	arg2Of	NF,(
R89	T159	T157	arg3Of	),(
R90	T160	T156	arg1Of	-κB,factor
R91	T160	T161	arg1Of	-κB,is
R92	T160	T169	arg1Of	-κB,is
R93	T160	T170	arg2Of	-κB,upregulated
R94	T161	T168	arg1Of	is,and
R95	T164	T161	arg2Of	regulator,is
R96	T164	T162	arg1Of	regulator,a
R97	T164	T163	arg1Of	regulator,master
R98	T164	T165	arg1Of	regulator,of
R99	T167	T165	arg2Of	genes,of
R100	T167	T166	arg1Of	genes,pro-inflammatory
R101	T170	T168	arg2Of	upregulated,and
R102	T170	T169	arg2Of	upregulated,is
R103	T170	T171	arg1Of	upregulated,in
R104	T174	T172	arg1Of	virus,human
R105	T174	T173	arg1Of	virus,immunodeficiency
R106	T174	T175	arg1Of	virus,1
R107	T174	T176	arg1Of	virus,(
R108	T177	T176	arg2Of	HIV-1,(
R109	T178	T176	arg3Of	),(
R110	T179	T171	arg2Of	infection,in
R111	T179	T174	arg1Of	infection,virus
R112	T180	T181	arg1Of	Mechanisms,underlying
R113	T180	T187	arg1Of	Mechanisms,are
R114	T180	T188	arg1Of	Mechanisms,relevant
R115	T184	T181	arg2Of	deregulation,underlying
R116	T184	T182	arg1Of	deregulation,the
R117	T184	T183	arg1Of	deregulation,NF-κB
R118	T184	T185	arg1Of	deregulation,by
R119	T186	T185	arg2Of	HIV-1,by
R120	T188	T187	arg2Of	relevant,are
R121	T188	T189	arg1Of	relevant,for
R122	T191	T189	arg2Of	dysfunction,for
R123	T191	T190	arg1Of	dysfunction,immune
R124	T191	T192	arg1Of	dysfunction,in
R125	T193	T192	arg2Of	AIDS,in
R126	T194	T195	arg1Of	We,report
R127	T201	T198	arg1Of	infection,single
R128	T201	T199	arg1Of	infection,round
R129	T201	T200	arg1Of	infection,HIV-1
R130	T201	T203	arg1Of	infection,or
R131	T203	T197	arg2Of	or,in
R132	T203	T202	arg1Of	or,","
R133	T206	T203	arg2Of	stimulation,or
R134	T206	T204	arg1Of	stimulation,single-pulse
R135	T206	T205	arg1Of	stimulation,PMA
R136	T211	T208	arg1Of	transactivator,the
R137	T211	T209	arg1Of	transactivator,HIV-1
R138	T211	T210	arg1Of	transactivator,Tat
R139	T211	T212	arg1Of	transactivator,activated
R140	T211	T215	arg1Of	transactivator,hijacking
R141	T211	T221	arg1Of	transactivator,preventing
R142	T212	T195	arg2Of	activated,report
R143	T212	T196	arg1Of	activated,that
R144	T212	T197	arg1Of	activated,in
R145	T212	T207	arg1Of	activated,","
R146	T212	T214	arg1Of	activated,by
R147	T212	T220	arg1Of	activated,by
R148	T213	T212	arg2Of	NF-κB,activated
R149	T214	T219	arg1Of	by,and
R150	T215	T214	arg2Of	hijacking,by
R151	T218	T215	arg2Of	IκB-α,hijacking
R152	T218	T216	arg1Of	IκB-α,the
R153	T218	T217	arg1Of	IκB-α,inhibitor
R154	T220	T219	arg2Of	by,and
R155	T221	T220	arg2Of	preventing,by
R156	T224	T221	arg2Of	binding,preventing
R157	T224	T222	arg1Of	binding,the
R158	T224	T223	arg1Of	binding,repressor
R159	T224	T225	arg1Of	binding,to
R160	T228	T225	arg2Of	complex,to
R161	T228	T226	arg1Of	complex,the
R162	T228	T227	arg1Of	complex,NF-κB
R163	T231	T232	arg1Of	Tat,associated
R164	T231	T240	arg1Of	Tat,increased
R165	T232	T233	arg1Of	associated,with
R166	T232	T239	arg1Of	associated,and
R167	T236	T233	arg2Of	subunit,with
R168	T236	T234	arg1Of	subunit,the
R169	T236	T235	arg1Of	subunit,p65
R170	T236	T237	arg1Of	subunit,of
R171	T238	T237	arg2Of	NF-κB,of
R172	T239	T229	arg1Of	and,Moreover
R173	T239	T230	arg1Of	and,","
R174	T240	T239	arg2Of	increased,and
R175	T244	T242	arg1Of	affinity,p65
R176	T244	T243	arg1Of	affinity,DNA-binding
R177	T244	T245	arg1Of	affinity,and
R178	T245	T240	arg2Of	and,increased
R179	T245	T241	arg1Of	and,the
R180	T247	T245	arg2Of	activity,and
R181	T247	T246	arg1Of	activity,transcriptional
R182	T249	T250	arg1Of	arginine-,and
R183	T251	T250	arg2Of	cysteine-rich,and
R184	T252	T248	arg1Of	domains,The
R185	T252	T249	arg1Of	domains,arginine-
R186	T252	T251	arg1Of	domains,cysteine-rich
R187	T252	T253	arg1Of	domains,of
R188	T252	T255	arg1Of	domains,were
R189	T252	T256	arg2Of	domains,required
R190	T254	T253	arg2Of	Tat,of
R191	T256	T255	arg2Of	required,were
R192	T256	T257	arg1Of	required,for
R193	T256	T262	arg1Of	required,","
R194	T256	T263	arg1Of	required,respectively
R195	T256	T264	arg1Of	required,","
R196	T256	T265	arg1Of	required,and
R197	T258	T259	arg1Of	IκB-α,and
R198	T259	T257	arg2Of	and,for
R199	T261	T259	arg2Of	association,and
R200	T261	T260	arg1Of	association,p65
R201	T266	T256	arg3Of	for,required
R202	T267	T266	arg2Of	sustaining,for
R203	T270	T267	arg2Of	activity,sustaining
R204	T270	T268	arg1Of	activity,the
R205	T270	T269	arg1Of	activity,NF-κB
R206	T273	T271	arg2Of	array,Among
R207	T273	T272	arg1Of	array,an
R208	T273	T274	arg1Of	array,of
R209	T276	T274	arg2Of	genes,of
R210	T276	T275	arg1Of	genes,NF-κB-responsive
R211	T278	T280	arg1Of	Tat,activated
R212	T278	T290	arg1Of	Tat,bound
R213	T280	T284	arg1Of	activated,in
R214	T280	T289	arg1Of	activated,and
R215	T283	T280	arg2Of	expression,activated
R216	T283	T281	arg1Of	expression,the
R217	T283	T282	arg1Of	expression,MIP-1α
R218	T287	T284	arg2Of	manner,in
R219	T287	T285	arg1Of	manner,a
R220	T287	T286	arg1Of	manner,p65-dependent
R221	T289	T271	arg1Of	and,Among
R222	T289	T277	arg1Of	and,","
R223	T289	T279	arg1Of	and,mostly
R224	T289	T288	arg1Of	and,","
R225	T290	T289	arg2Of	bound,and
R226	T290	T291	arg1Of	bound,to
R227	T295	T291	arg2Of	enhancer,to
R228	T295	T292	arg1Of	enhancer,the
R229	T295	T293	arg1Of	enhancer,MIP-1α
R230	T295	T294	arg1Of	enhancer,NF-κB
R231	T295	T297	arg1Of	enhancer,promoting
R232	T297	T296	arg1Of	promoting,thus
R233	T297	T306	arg1Of	promoting,;
R234	T299	T297	arg2Of	recruitment,promoting
R235	T299	T298	arg1Of	recruitment,the
R236	T299	T300	arg1Of	recruitment,of
R237	T299	T302	arg1Of	recruitment,with
R238	T301	T300	arg2Of	p65,of
R239	T303	T302	arg2Of	displacement,with
R240	T303	T304	arg1Of	displacement,of
R241	T305	T304	arg2Of	IκB-α,of
R242	T308	T307	arg1Of	findings,similar
R243	T308	T309	arg1Of	findings,were
R244	T308	T310	arg2Of	findings,obtained
R245	T310	T306	arg2Of	obtained,;
R246	T310	T309	arg2Of	obtained,were
R247	T310	T311	arg1Of	obtained,for
R248	T314	T312	arg1Of	genes,the
R249	T314	T313	arg1Of	genes,NF-κB-responsive
R250	T315	T316	arg1Of	CSF3,","
R251	T316	T318	arg1Of	",",","
R252	T317	T316	arg2Of	LTA,","
R253	T318	T320	arg1Of	",",and
R254	T319	T318	arg2Of	NFKBIA,","
R255	T320	T311	arg2Of	and,for
R256	T320	T314	arg1Of	and,genes
R257	T321	T320	arg2Of	TLR2,and
R258	T323	T322	arg1Of	results,Our
R259	T323	T324	arg1Of	results,support
R260	T323	T342	arg1Of	results,may
R261	T323	T343	arg1Of	results,contribute
R262	T324	T341	arg1Of	support,and
R263	T327	T324	arg2Of	mechanism,support
R264	T327	T325	arg1Of	mechanism,a
R265	T327	T326	arg1Of	mechanism,novel
R266	T327	T328	arg1Of	mechanism,of
R267	T327	T331	arg1Of	mechanism,via
R268	T330	T328	arg2Of	activation,of
R269	T330	T329	arg1Of	activation,NF-κB
R270	T333	T331	arg2Of	interaction,via
R271	T333	T332	arg1Of	interaction,physical
R272	T333	T334	arg1Of	interaction,of
R273	T333	T336	arg1Of	interaction,with
R274	T335	T334	arg2Of	Tat,of
R275	T337	T338	arg1Of	IκB-α,and
R276	T338	T336	arg2Of	and,with
R277	T339	T338	arg2Of	p65,and
R278	T341	T340	arg1Of	and,","
R279	T343	T341	arg2Of	contribute,and
R280	T343	T342	arg2Of	contribute,may
R281	T343	T344	arg1Of	contribute,to
R282	T346	T344	arg2Of	insights,to
R283	T346	T345	arg1Of	insights,further
R284	T346	T347	arg1Of	insights,into
R285	T349	T347	arg2Of	deregulation,into
R286	T349	T348	arg1Of	deregulation,the
R287	T349	T350	arg1Of	deregulation,of
R288	T349	T354	arg1Of	deregulation,by
R289	T353	T350	arg2Of	response,of
R290	T353	T351	arg1Of	response,the
R291	T353	T352	arg1Of	response,inflammatory
R292	T355	T354	arg2Of	HIV-1,by
R1275	T1457	T1456	arg2Of	NF,(
R1276	T1458	T1456	arg3Of	),(
R1279	T1461	T1454	arg1Of	factors,Nuclear
R1280	T1461	T1455	arg1Of	factors,factor
R1281	T1461	T1456	arg1Of	factors,(
R1282	T1461	T1459	arg1Of	factors,-κB
R1285	T1464	T1465	arg1Of	transcription,of
R1286	T1466	T1465	arg2Of	genes,of
R1287	T1466	T1467	arg1Of	genes,that
R1288	T1466	T1468	arg1Of	genes,are
R1289	T1466	T1469	arg2Of	genes,involved
R1290	T1469	T1468	arg2Of	involved,are
R1291	T1469	T1470	arg1Of	involved,in
R1292	T1472	T1473	arg1Of	immune,and
R1293	T1474	T1473	arg2Of	inflammatory,and
R1294	T1475	T1470	arg2Of	response,in
R1295	T1475	T1471	arg1Of	response,the
R1296	T1475	T1472	arg1Of	response,immune
R1297	T1475	T1474	arg1Of	response,inflammatory
R1298	T1475	T1476	arg1Of	response,(
R1299	T1477	T1476	arg2Of	1,(
R1300	T1478	T1476	arg3Of	),(
R1301	T1481	T1479	arg1Of	family,The
R1302	T1481	T1480	arg1Of	family,NF-κB
R1303	T1481	T1482	arg1Of	family,includes
R1304	T1483	T1484	arg1Of	RelA/p65,","
R1305	T1484	T1486	arg1Of	",",","
R1306	T1485	T1484	arg2Of	c-Rel,","
R1307	T1486	T1488	arg1Of	",",","
R1308	T1487	T1486	arg2Of	RelB,","
R1309	T1488	T1482	arg2Of	",",includes
R1310	T1488	T1492	arg1Of	",",that
R1311	T1488	T1493	arg1Of	",",share
R1312	T1489	T1490	arg1Of	p50,and
R1313	T1490	T1488	arg2Of	and,","
R1314	T1491	T1490	arg2Of	p52,and
R1315	T1493	T1505	arg1Of	share,for
R1316	T1496	T1495	arg1Of	conserved,highly
R1317	T1501	T1493	arg2Of	domain,share
R1318	T1501	T1494	arg1Of	domain,a
R1319	T1501	T1496	arg1Of	domain,conserved
R1320	T1501	T1497	arg1Of	domain,300-amino
R1321	T1501	T1498	arg1Of	domain,acid
R1322	T1501	T1499	arg1Of	domain,Rel
R1323	T1501	T1500	arg1Of	domain,homology
R1324	T1501	T1502	arg1Of	domain,(
R1325	T1503	T1502	arg2Of	RHD,(
R1326	T1504	T1502	arg3Of	),(
R1327	T1506	T1507	arg1Of	homo-,or
R1328	T1507	T1505	arg2Of	or,for
R1329	T1508	T1509	arg1Of	hetero-dimerization,and
R1330	T1509	T1507	arg2Of	and,or
R1331	T1510	T1509	arg2Of	DNA-binding,and
R1332	T1513	T1511	arg1Of	activity,The
R1333	T1513	T1512	arg1Of	activity,transcriptional
R1334	T1513	T1514	arg1Of	activity,of
R1335	T1513	T1518	arg1Of	activity,depends
R1336	T1517	T1514	arg2Of	complex,of
R1337	T1517	T1515	arg1Of	complex,the
R1338	T1517	T1516	arg1Of	complex,NF-κB
R1339	T1518	T1519	arg1Of	depends,on
R1340	T1518	T1522	arg1Of	depends,since
R1341	T1521	T1519	arg2Of	composition,on
R1342	T1521	T1520	arg1Of	composition,dimer
R1343	T1527	T1523	arg1Of	domains,C-terminal
R1344	T1527	T1524	arg1Of	domains,unrelated
R1345	T1527	T1525	arg1Of	domains,transcriptional
R1346	T1527	T1526	arg1Of	domains,activation
R1347	T1527	T1528	arg1Of	domains,are
R1348	T1527	T1529	arg1Of	domains,present
R1349	T1528	T1522	arg2Of	are,since
R1350	T1529	T1528	arg2Of	present,are
R1351	T1529	T1531	arg1Of	present,in
R1352	T1531	T1530	arg1Of	in,exclusively
R1353	T1532	T1533	arg1Of	p65,","
R1354	T1533	T1535	arg1Of	",",and
R1355	T1534	T1533	arg2Of	RelB,","
R1356	T1535	T1531	arg2Of	and,in
R1357	T1536	T1535	arg2Of	c-Rel,and
R1358	T1536	T1537	arg1Of	c-Rel,(
R1359	T1538	T1537	arg2Of	2,(
R1360	T1539	T1537	arg3Of	),(
R1361	T1540	T1541	arg1Of	Inhibitors,of
R1362	T1540	T1546	arg1Of	Inhibitors,associate
R1363	T1540	T1552	arg1Of	Inhibitors,interfere
R1364	T1542	T1541	arg2Of	NF-κB,of
R1365	T1542	T1543	arg1Of	NF-κB,(
R1366	T1544	T1543	arg2Of	IκB,(
R1367	T1545	T1543	arg3Of	),(
R1368	T1546	T1547	arg1Of	associate,with
R1369	T1546	T1551	arg1Of	associate,and
R1370	T1550	T1547	arg2Of	complex,with
R1371	T1550	T1548	arg1Of	complex,the
R1372	T1550	T1549	arg1Of	complex,NF-κB
R1373	T1552	T1551	arg2Of	interfere,and
R1374	T1552	T1553	arg1Of	interfere,with
R1375	T1555	T1553	arg2Of	binding,with
R1376	T1555	T1554	arg1Of	binding,its
R1377	T1555	T1556	arg1Of	binding,to
R1378	T1557	T1556	arg2Of	DNA,to
R1379	T1557	T1558	arg1Of	DNA,(
R1380	T1559	T1558	arg2Of	3,(
R1381	T1560	T1558	arg3Of	),(
R1382	T1564	T1561	arg2Of	pathway,In
R1383	T1564	T1562	arg1Of	pathway,the
R1384	T1564	T1563	arg1Of	pathway,canonical
R1385	T1564	T1565	arg1Of	pathway,of
R1386	T1567	T1565	arg2Of	activation,of
R1387	T1567	T1566	arg1Of	activation,NF-κB
R1388	T1572	T1569	arg1Of	kinase,the
R1389	T1572	T1570	arg2Of	kinase,activated
R1390	T1572	T1571	arg1Of	kinase,IκB
R1391	T1572	T1573	arg1Of	kinase,(
R1392	T1572	T1576	arg1Of	kinase,phosphorylates
R1393	T1574	T1573	arg2Of	IKK,(
R1394	T1575	T1573	arg3Of	),(
R1395	T1576	T1561	arg1Of	phosphorylates,In
R1396	T1576	T1568	arg1Of	phosphorylates,","
R1397	T1576	T1578	arg1Of	phosphorylates,at
R1398	T1577	T1576	arg2Of	IκB,phosphorylates
R1399	T1581	T1578	arg2Of	residues,at
R1400	T1581	T1579	arg1Of	residues,specific
R1401	T1581	T1580	arg1Of	residues,serine
R1402	T1581	T1582	arg1Of	residues,that
R1403	T1581	T1583	arg1Of	residues,target
R1404	T1585	T1583	arg2Of	protein,target
R1405	T1585	T1584	arg1Of	protein,the
R1406	T1585	T1586	arg1Of	protein,to
R1407	T1587	T1588	arg1Of	ubiquitination,and
R1408	T1588	T1586	arg2Of	and,to
R1409	T1588	T1591	arg1Of	and,","
R1410	T1588	T1592	arg1Of	and,which
R1411	T1588	T1593	arg1Of	and,releases
R1412	T1590	T1588	arg2Of	degradation,and
R1413	T1590	T1589	arg1Of	degradation,proteasomal
R1414	T1597	T1593	arg2Of	complex,releases
R1415	T1597	T1594	arg1Of	complex,the
R1416	T1597	T1595	arg1Of	complex,functional
R1417	T1597	T1596	arg1Of	complex,NF-κB
R1418	T1597	T1598	arg1Of	complex,in
R1419	T1600	T1598	arg2Of	nucleus,in
R1420	T1600	T1599	arg1Of	nucleus,the
R1421	T1601	T1602	arg1Of	IκB-α,","
R1422	T1601	T1613	arg1Of	IκB-α,is
R1423	T1601	T1614	arg2Of	IκB-α,phosphorylated
R1424	T1601	T1627	arg2Of	IκB-α,ubiquitylated
R1425	T1605	T1604	arg1Of	abundant,most
R1426	T1606	T1602	arg2Of	inhibitor,","
R1427	T1606	T1603	arg1Of	inhibitor,the
R1428	T1606	T1605	arg1Of	inhibitor,abundant
R1429	T1606	T1607	arg1Of	inhibitor,of
R1430	T1608	T1607	arg2Of	NF-κB,of
R1431	T1608	T1609	arg1Of	NF-κB,(
R1432	T1610	T1609	arg2Of	4,(
R1433	T1611	T1609	arg3Of	),(
R1434	T1614	T1625	arg1Of	phosphorylated,and
R1435	T1616	T1614	arg1Of	IKK,phosphorylated
R1436	T1616	T1615	arg2Of	IKK,by
R1437	T1616	T1617	arg1Of	IKK,at
R1438	T1618	T1619	arg1Of	Ser32,and
R1439	T1619	T1617	arg2Of	and,at
R1440	T1620	T1619	arg2Of	Ser36,and
R1441	T1620	T1621	arg1Of	Ser36,(
R1442	T1622	T1621	arg2Of	5,(
R1443	T1623	T1621	arg3Of	),(
R1444	T1625	T1612	arg1Of	and,","
R1445	T1625	T1613	arg2Of	and,is
R1446	T1625	T1624	arg1Of	and,","
R1447	T1627	T1625	arg2Of	ubiquitylated,and
R1448	T1627	T1626	arg1Of	ubiquitylated,subsequently
R1449	T1627	T1628	arg1Of	ubiquitylated,at
R1450	T1629	T1630	arg1Of	Lys21,and
R1451	T1630	T1628	arg2Of	and,at
R1452	T1630	T1632	modOf	and,to
R1453	T1630	T1633	arg1Of	and,be
R1454	T1630	T1634	arg2Of	and,degraded
R1455	T1631	T1630	arg2Of	Lys22,and
R1456	T1634	T1632	arg1Of	degraded,to
R1457	T1634	T1633	arg2Of	degraded,be
R1458	T1638	T1634	arg1Of	proteasome,degraded
R1459	T1638	T1635	arg2Of	proteasome,by
R1460	T1638	T1636	arg1Of	proteasome,the
R1461	T1638	T1637	arg1Of	proteasome,26S
R1462	T1638	T1639	arg1Of	proteasome,(
R1463	T1640	T1639	arg2Of	6,(
R1464	T1641	T1639	arg3Of	),(
R1465	T1644	T1642	arg1Of	activity,The
R1466	T1644	T1643	arg1Of	activity,NF-κB
R1467	T1644	T1645	arg1Of	activity,is
R1468	T1644	T1646	arg2Of	activity,enhanced
R1469	T1646	T1645	arg2Of	enhanced,is
R1470	T1646	T1647	arg1Of	enhanced,by
R1471	T1646	T1651	arg1Of	enhanced,at
R1472	T1648	T1647	arg2Of	phosphorylation,by
R1473	T1648	T1649	arg1Of	phosphorylation,of
R1474	T1650	T1649	arg2Of	p65,of
R1475	T1652	T1651	arg2Of	Ser276,at
R1476	T1654	T1655	arg1Of	PKA,and
R1477	T1655	T1660	arg1Of	and,","
R1478	T1656	T1655	arg2Of	MSK1,and
R1479	T1656	T1657	arg1Of	MSK1,(
R1480	T1658	T1657	arg2Of	"7,8",(
R1481	T1659	T1657	arg3Of	),(
R1482	T1660	T1646	arg1Of	",",enhanced
R1483	T1660	T1653	arg2Of	",",by
R1484	T1660	T1662	arg1Of	",",by
R1485	T1661	T1660	arg2Of	Ser311,","
R1486	T1663	T1664	arg1Of	PKCζ,(
R1487	T1663	T1667	arg1Of	PKCζ,and
R1488	T1665	T1664	arg2Of	9,(
R1489	T1666	T1664	arg3Of	),(
R1490	T1667	T1662	arg2Of	and,by
R1491	T1667	T1669	arg1Of	and,by
R1492	T1668	T1667	arg2Of	Ser536,and
R1493	T1670	T1669	arg2Of	IKKα,by
R1494	T1670	T1671	arg1Of	IKKα,(
R1495	T1672	T1671	arg2Of	"10,11",(
R1496	T1673	T1671	arg3Of	),(
R1497	T1674	T1675	arg1Of	Acetylation,of
R1498	T1674	T1677	arg1Of	Acetylation,at
R1499	T1674	T1681	arg1Of	Acetylation,increases
R1500	T1674	T1686	arg1Of	Acetylation,impairs
R1501	T1676	T1675	arg2Of	p65,of
R1502	T1678	T1679	arg1Of	Lys218,and
R1503	T1679	T1677	arg2Of	and,at
R1504	T1680	T1679	arg2Of	Lys221,and
R1505	T1681	T1685	arg1Of	increases,and
R1506	T1684	T1681	arg2Of	binding,increases
R1507	T1684	T1682	arg1Of	binding,the
R1508	T1684	T1683	arg1Of	binding,DNA
R1509	T1685	T1692	arg1Of	and,and
R1510	T1686	T1685	arg2Of	impairs,and
R1511	T1688	T1686	arg2Of	association,impairs
R1512	T1688	T1687	arg1Of	association,the
R1513	T1688	T1689	arg1Of	association,with
R1514	T1690	T1689	arg2Of	IκB-α,with
R1515	T1692	T1691	arg1Of	and,","
R1516	T1693	T1694	arg1Of	acetylation,at
R1517	T1693	T1696	arg1Of	acetylation,enhances
R1518	T1695	T1694	arg2Of	Lys310,at
R1519	T1696	T1692	arg2Of	enhances,and
R1520	T1700	T1696	arg2Of	activity,enhances
R1521	T1700	T1697	arg1Of	activity,the
R1522	T1700	T1698	arg1Of	activity,p65
R1523	T1700	T1699	arg1Of	activity,transcriptional
R1524	T1700	T1701	arg1Of	activity,(
R1525	T1702	T1701	arg2Of	"12,13",(
R1526	T1703	T1701	arg3Of	),(
R1527	T1705	T1704	arg1Of	turn,Post-activation
R1528	T1705	T1706	arg1Of	turn,off
R1529	T1708	T1707	arg1Of	NF-κB,of
R1530	T1708	T1709	arg1Of	NF-κB,is
R1531	T1708	T1710	arg2Of	NF-κB,regulated
R1532	T1710	T1705	arg2Of	regulated,turn
R1533	T1710	T1709	arg2Of	regulated,is
R1534	T1710	T1715	arg1Of	regulated,through
R1535	T1714	T1710	arg1Of	loop,regulated
R1536	T1714	T1711	arg2Of	loop,by
R1537	T1714	T1712	arg1Of	loop,negative
R1538	T1714	T1713	arg1Of	loop,feedback
R1539	T1716	T1715	arg2Of	inhibitors,through
R1540	T1716	T1717	arg1Of	inhibitors,under
R1541	T1716	T1723	arg1Of	inhibitors,","
R1542	T1716	T1725	arg1Of	inhibitors,as
R1543	T1720	T1717	arg2Of	control,under
R1544	T1720	T1718	arg1Of	control,the
R1545	T1720	T1719	arg1Of	control,transcriptional
R1546	T1720	T1721	arg1Of	control,of
R1547	T1722	T1721	arg2Of	NF-κB,of
R1548	T1725	T1724	arg1Of	as,such
R1549	T1726	T1727	arg1Of	IκB-α,and
R1550	T1728	T1727	arg2Of	ubiquitin-editing,and
R1551	T1730	T1725	arg2Of	A20,as
R1552	T1730	T1726	arg1Of	A20,IκB-α
R1553	T1730	T1728	arg1Of	A20,ubiquitin-editing
R1554	T1730	T1729	arg1Of	A20,protein
R1555	T1730	T1731	arg1Of	A20,(
R1556	T1732	T1731	arg2Of	14–20,(
R1557	T1733	T1731	arg3Of	),(
R1558	T1734	T1735	arg1Of	Deacetylation,of
R1559	T1734	T1737	arg1Of	Deacetylation,by
R1560	T1734	T1743	arg1Of	Deacetylation,or
R1561	T1736	T1735	arg2Of	p65,of
R1562	T1739	T1740	arg1Of	deacetylase-3,or
R1563	T1740	T1737	arg2Of	or,by
R1564	T1740	T1738	arg1Of	or,histone
R1565	T1741	T1740	arg2Of	SIRT1,or
R1566	T1743	T1742	arg1Of	or,","
R1567	T1743	T1751	arg1Of	or,down-regulate
R1568	T1744	T1743	arg2Of	acetylation,or
R1569	T1744	T1745	arg1Of	acetylation,of
R1570	T1744	T1747	arg1Of	acetylation,at
R1571	T1746	T1745	arg2Of	p65,of
R1572	T1748	T1749	arg1Of	Lys122,and
R1573	T1749	T1747	arg2Of	and,at
R1574	T1750	T1749	arg2Of	Lys123,and
R1575	T1754	T1751	arg2Of	activity,down-regulate
R1576	T1754	T1752	arg1Of	activity,the
R1577	T1754	T1753	arg1Of	activity,NF-κB
R1578	T1754	T1755	arg1Of	activity,(
R1579	T1756	T1755	arg2Of	"12,21,22",(
R1580	T1757	T1755	arg3Of	),(
R1581	T1759	T1758	arg1Of	activation,Persistent
R1582	T1759	T1760	arg1Of	activation,of
R1583	T1759	T1762	arg1Of	activation,occurs
R1584	T1759	T1778	arg1Of	activation,enhances
R1585	T1761	T1760	arg2Of	NF-κB,of
R1586	T1762	T1763	arg1Of	occurs,in
R1587	T1762	T1777	arg1Of	occurs,and
R1588	T1766	T1764	arg1Of	virus-1,human
R1589	T1766	T1765	arg1Of	virus-1,immunodeficiency
R1590	T1766	T1767	arg1Of	virus-1,(
R1591	T1768	T1767	arg2Of	HIV-1,(
R1592	T1769	T1767	arg3Of	),(
R1593	T1771	T1772	arg1Of	monocytes,","
R1594	T1772	T1774	arg1Of	",",and
R1595	T1773	T1772	arg2Of	macrophages,","
R1596	T1774	T1763	arg2Of	and,in
R1597	T1774	T1766	arg1Of	and,virus-1
R1598	T1774	T1770	arg1Of	and,-infected
R1599	T1775	T1774	arg2Of	microglia,and
R1600	T1777	T1776	arg1Of	and,","
R1601	T1778	T1777	arg2Of	enhances,and
R1602	T1780	T1778	arg2Of	expression,enhances
R1603	T1780	T1779	arg1Of	expression,the
R1604	T1780	T1781	arg1Of	expression,of
R1605	T1783	T1781	arg2Of	genes,of
R1606	T1783	T1782	arg1Of	genes,NF-κB-responsive
R1607	T1783	T1784	arg1Of	genes,","
R1608	T1783	T1785	arg1Of	genes,including
R1609	T1787	T1786	arg1Of	cytokines,pro-inflammatory
R1610	T1787	T1788	arg1Of	cytokines,","
R1611	T1788	T1792	arg1Of	",",and
R1612	T1791	T1788	arg2Of	molecules,","
R1613	T1791	T1789	arg1Of	molecules,cell
R1614	T1791	T1790	arg1Of	molecules,adhesion
R1615	T1792	T1785	arg2Of	and,including
R1616	T1793	T1792	arg2Of	chemokines,and
R1617	T1793	T1794	arg1Of	chemokines,(
R1618	T1795	T1794	arg2Of	23–25,(
R1619	T1796	T1794	arg3Of	),(
R1620	T1798	T1797	arg1Of	inflammation,Chronic
R1621	T1798	T1799	arg1Of	inflammation,is
R1622	T1799	T1808	arg1Of	is,in
R1623	T1802	T1799	arg2Of	cause,is
R1624	T1802	T1800	arg1Of	cause,a
R1625	T1802	T1801	arg1Of	cause,major
R1626	T1802	T1803	arg1Of	cause,of
R1627	T1807	T1803	arg2Of	dysfunction,of
R1628	T1807	T1804	arg1Of	dysfunction,immune
R1629	T1807	T1805	arg1Of	dysfunction,and
R1630	T1807	T1806	arg1Of	dysfunction,neuron
R1631	T1809	T1808	arg2Of	AIDS,in
R1632	T1809	T1810	arg1Of	AIDS,(
R1633	T1811	T1810	arg2Of	"26,27",(
R1634	T1812	T1810	arg3Of	),(
R1635	T1817	T1815	arg1Of	hosts,non-human
R1636	T1817	T1816	arg1Of	hosts,primate
R1637	T1817	T1818	arg1Of	hosts,for
R1638	T1817	T1822	arg1Of	hosts,","
R1639	T1817	T1824	arg1Of	hosts,as
R1640	T1817	T1832	arg1Of	hosts,lack
R1641	T1817	T1837	arg1Of	hosts,do
R1642	T1817	T1839	arg1Of	hosts,develop
R1643	T1821	T1818	arg2Of	virus,for
R1644	T1821	T1819	arg1Of	virus,simian
R1645	T1821	T1820	arg1Of	virus,immunodeficiency
R1646	T1824	T1823	arg1Of	as,such
R1647	T1827	T1825	arg1Of	monkeys,African
R1648	T1827	T1826	arg1Of	monkeys,green
R1649	T1827	T1828	arg1Of	monkeys,and
R1650	T1828	T1824	arg2Of	and,as
R1651	T1830	T1828	arg2Of	mangabey,and
R1652	T1830	T1829	arg1Of	mangabey,sooty
R1653	T1832	T1836	arg1Of	lack,and
R1654	T1835	T1832	arg2Of	activation,lack
R1655	T1835	T1833	arg1Of	activation,aberrant
R1656	T1835	T1834	arg1Of	activation,immune
R1657	T1836	T1813	arg1Of	and,Consistently
R1658	T1836	T1814	arg1Of	and,","
R1659	T1836	T1831	arg1Of	and,","
R1660	T1839	T1836	arg2Of	develop,and
R1661	T1839	T1837	arg2Of	develop,do
R1662	T1839	T1838	arg1Of	develop,not
R1663	T1839	T1841	arg1Of	develop,despite
R1664	T1840	T1839	arg2Of	AIDS,develop
R1665	T1844	T1841	arg2Of	replication,despite
R1666	T1844	T1842	arg1Of	replication,high
R1667	T1844	T1843	arg1Of	replication,virus
R1668	T1844	T1845	arg1Of	replication,(
R1669	T1846	T1845	arg2Of	"28,29",(
R1670	T1847	T1845	arg3Of	),(
R1671	T1850	T1858	arg1Of	understanding,may
R1672	T1850	T1859	arg1Of	understanding,provide
R1673	T1852	T1850	arg2Of	mechanisms,understanding
R1674	T1852	T1851	arg1Of	mechanisms,the
R1675	T1852	T1853	arg1Of	mechanisms,of
R1676	T1852	T1856	arg1Of	mechanisms,by
R1677	T1855	T1853	arg2Of	deregulation,of
R1678	T1855	T1854	arg1Of	deregulation,NF-κB
R1679	T1857	T1856	arg2Of	HIV-1,by
R1680	T1859	T1848	arg1Of	provide,Thus
R1681	T1859	T1849	arg1Of	provide,","
R1682	T1859	T1858	arg2Of	provide,may
R1683	T1859	T1862	arg1Of	provide,into
R1684	T1861	T1859	arg2Of	insights,provide
R1685	T1861	T1860	arg1Of	insights,further
R1686	T1864	T1862	arg2Of	pathogenesis,into
R1687	T1864	T1863	arg1Of	pathogenesis,AIDS
R1688	T1867	T1865	arg2Of	entry,In
R1689	T1867	T1866	arg1Of	entry,HIV-1
R1690	T1870	T1869	arg1Of	binding,the
R1691	T1870	T1871	arg1Of	binding,of
R1692	T1870	T1876	arg1Of	binding,to
R1693	T1870	T1878	arg1Of	binding,induces
R1694	T1875	T1871	arg2Of	envelope,of
R1695	T1875	T1872	arg1Of	envelope,the
R1696	T1875	T1873	arg1Of	envelope,gp120
R1697	T1875	T1874	arg1Of	envelope,viral
R1698	T1877	T1876	arg2Of	CD4,to
R1699	T1878	T1865	arg1Of	induces,In
R1700	T1878	T1868	arg1Of	induces,","
R1701	T1878	T1882	arg1Of	induces,by
R1702	T1881	T1878	arg2Of	activity,induces
R1703	T1881	T1879	arg1Of	activity,the
R1704	T1881	T1880	arg1Of	activity,NF-κB
R1705	T1883	T1884	arg1Of	activation,of
R1706	T1883	T1889	arg1Of	activation,and
R1707	T1885	T1884	arg2Of	IKK,of
R1708	T1885	T1886	arg1Of	IKK,(
R1709	T1887	T1886	arg2Of	30,(
R1710	T1888	T1886	arg3Of	),(
R1711	T1889	T1882	arg2Of	and,by
R1712	T1890	T1889	arg2Of	procaspase,and
R1713	T1890	T1891	arg1Of	procaspase,8
R1714	T1890	T1892	arg1Of	procaspase,(
R1715	T1893	T1892	arg2Of	31,(
R1716	T1894	T1892	arg3Of	),(
R1717	T1897	T1895	arg2Of	integration,Following
R1718	T1897	T1896	arg1Of	integration,viral
R1719	T1904	T1899	arg1Of	protein,the
R1720	T1904	T1900	arg1Of	protein,early
R1721	T1904	T1901	arg2Of	protein,encoded
R1722	T1904	T1902	arg1Of	protein,HIV-1
R1723	T1904	T1903	arg1Of	protein,Tat
R1724	T1904	T1905	arg1Of	protein,interacts
R1725	T1904	T1915	arg1Of	protein,sustain
R1726	T1905	T1895	arg1Of	interacts,Following
R1727	T1905	T1898	arg1Of	interacts,","
R1728	T1905	T1906	arg1Of	interacts,with
R1729	T1909	T1908	arg1Of	RNA,HIV-1
R1730	T1909	T1910	arg1Of	RNA,and
R1731	T1910	T1906	arg2Of	and,with
R1732	T1910	T1907	arg1Of	and,the
R1733	T1913	T1910	arg2Of	factors,and
R1734	T1913	T1911	arg1Of	factors,host
R1735	T1913	T1912	arg1Of	factors,cell
R1736	T1915	T1905	arg2Of	sustain,interacts
R1737	T1915	T1914	arg1Of	sustain,to
R1738	T1918	T1915	arg2Of	replication,sustain
R1739	T1918	T1916	arg1Of	replication,the
R1740	T1918	T1917	arg1Of	replication,viral
R1741	T1919	T1920	arg1Of	Tat,binds
R1742	T1920	T1921	arg1Of	binds,to
R1743	T1920	T1964	modOf	binds,to
R1744	T1924	T1922	arg1Of	structures,RNA
R1745	T1924	T1923	arg1Of	structures,stem-loop
R1746	T1924	T1925	arg2Of	structures,generated
R1747	T1924	T1933	arg1Of	structures,","
R1748	T1924	T1934	arg1Of	structures,including
R1749	T1924	T1945	arg1Of	structures,","
R1750	T1925	T1926	arg1Of	generated,by
R1751	T1929	T1926	arg2Of	end,by
R1752	T1992	T1991	arg2Of	TFIID,","
R1753	T1992	T1993	arg1Of	TFIID,(
R1754	T1994	T1993	arg2Of	36,(
R1755	T1929	T1927	arg1Of	end,the
R1756	T1995	T1993	arg3Of	),(
R1757	T1996	T2001	arg1Of	",",","
R1758	T1929	T1928	arg1Of	end,5′
R1759	T1997	T1996	arg2Of	E2F-4,","
R1760	T1997	T1998	arg1Of	E2F-4,(
R1761	T1929	T1930	arg1Of	end,of
R1762	T1932	T1930	arg2Of	transcripts,of
R1763	T1999	T1998	arg2Of	37,(
R1764	T2000	T1998	arg3Of	),(
R1765	T2001	T1986	arg2Of	",",as
R1766	T1932	T1931	arg1Of	transcripts,target
R1767	T2002	T2003	arg1Of	C/EBPβ,(
R1768	T2002	T2006	arg1Of	C/EBPβ,","
R1769	T1938	T1934	arg2Of	element,including
R1770	T2004	T2003	arg2Of	38,(
R1771	T2005	T2003	arg3Of	),(
R1772	T2006	T2012	arg1Of	",",and
R1773	T2008	T2006	arg2Of	T1/CDK9,","
R1774	T2008	T2007	arg1Of	T1/CDK9,cyclin
R1775	T2008	T2009	arg1Of	T1/CDK9,(
R1776	T2010	T2009	arg2Of	"39,40",(
R1777	T2011	T2009	arg3Of	),(
R1778	T2012	T2001	arg2Of	and,","
R1779	T1938	T1935	arg1Of	element,the
R1780	T1938	T1936	arg1Of	element,HIV-1
R1781	T1938	T1937	arg1Of	element,transactivation-responsive
R1782	T2015	T2013	arg1Of	acetyltransferases,the
R1783	T2015	T2014	arg1Of	acetyltransferases,histone
R1784	T2016	T2015	arg1Of	p300/CBP,acetyltransferases
R1785	T2016	T2017	arg1Of	p300/CBP,and
R1786	T1938	T1939	arg1Of	element,(
R1787	T2017	T2012	arg2Of	and,and
R1788	T1938	T1942	arg1Of	element,(
R1789	T2018	T2017	arg2Of	P/CAF,and
R1790	T2018	T2019	arg1Of	P/CAF,(
R1791	T1940	T1939	arg2Of	TAR,(
R1792	T2020	T2019	arg2Of	41–43,(
R1793	T2021	T2019	arg3Of	),(
R1794	T2023	T2024	arg1Of	released,from
R1795	T2026	T2024	arg2Of	cells,from
R1796	T2026	T2025	arg1Of	cells,HIV-1-infected
R1797	T1941	T1939	arg3Of	),(
R1798	T2028	T2029	arg1Of	Tat,deregulates
R1799	T2028	T2034	arg1Of	Tat,binding
R1800	T2029	T2022	arg1Of	deregulates,When
R1801	T2029	T2023	modOf	deregulates,released
R1802	T2029	T2027	arg1Of	deregulates,","
R1803	T2029	T2033	arg1Of	deregulates,by
R1804	T1943	T1942	arg2Of	32,(
R1805	T2032	T2029	arg2Of	signaling,deregulates
R1806	T2032	T2030	arg1Of	signaling,the
R1807	T2032	T2031	arg1Of	signaling,cell
R1808	T2034	T2033	arg2Of	binding,by
R1809	T2034	T2035	arg1Of	binding,to
R1810	T1944	T1942	arg3Of	),(
R1811	T2037	T2035	arg2Of	receptors,to
R1812	T2037	T2036	arg1Of	receptors,cell
R1813	T2037	T2038	arg1Of	receptors,","
R1814	T2037	T2040	arg1Of	receptors,as
R1815	T1945	T1953	arg1Of	",",(
R1816	T2040	T2039	arg1Of	as,such
R1817	T2041	T2042	arg1Of	integrins,(
R1818	T2041	T2045	arg1Of	integrins,","
R1819	T1945	T1956	arg1Of	",",and
R1820	T2043	T2042	arg2Of	44,(
R1821	T2044	T2042	arg3Of	),(
R1822	T1949	T1945	arg2Of	β,","
R1823	T2045	T2051	arg1Of	",",and
R1824	T2047	T2045	arg2Of	receptor,","
R1825	T2047	T2046	arg1Of	receptor,Flk1/KDR
R1826	T2047	T2048	arg1Of	receptor,(
R1827	T2049	T2048	arg2Of	45,(
R1828	T2050	T2048	arg3Of	),(
R1829	T2051	T2040	arg2Of	and,as
R1830	T1949	T1946	arg1Of	β,tumor
R1831	T1949	T1947	arg1Of	β,necrosis
R1832	T2053	T2051	arg2Of	receptors,and
R1833	T2053	T2052	arg1Of	receptors,chemokine
R1834	T2053	T2054	arg1Of	receptors,(
R1835	T1949	T1948	arg1Of	β,factor
R1836	T2055	T2054	arg2Of	46,(
R1837	T1949	T1950	arg1Of	β,(
R1838	T1951	T1950	arg2Of	TNFβ,(
R1839	T2056	T2054	arg3Of	),(
R1840	T2057	T2059	arg1Of	We,reported
R1841	T2059	T2058	arg1Of	reported,first
R1842	T1952	T1950	arg3Of	),(
R1843	T2061	T2062	arg1Of	NF-κB,was
R1844	T2061	T2064	arg1Of	NF-κB,active
R1845	T2062	T2059	arg2Of	was,reported
R1846	T2062	T2060	arg1Of	was,that
R1847	T2064	T2062	arg2Of	active,was
R1848	T2064	T2063	arg1Of	active,constitutively
R1849	T2064	T2065	arg1Of	active,in
R1850	T2067	T2065	arg2Of	cells,in
R1851	T2067	T2066	arg1Of	cells,Jurkat
R1852	T2067	T2068	arg1Of	cells,that
R1853	T2067	T2070	arg1Of	cells,expressed
R1854	T1954	T1953	arg2Of	33,(
R1855	T2070	T2069	arg1Of	expressed,stably
R1856	T1955	T1953	arg3Of	),(
R1857	T1956	T1921	arg2Of	and,to
R1858	T2073	T2070	arg2Of	gene,expressed
R1859	T2073	T2071	arg1Of	gene,the
R1860	T2073	T2072	arg1Of	gene,Tat
R1861	T2073	T2074	arg1Of	gene,(
R1862	T1956	T1961	arg1Of	and,(
R1863	T2075	T2074	arg2Of	47,(
R1864	T1957	T1956	arg2Of	interleukin-6,and
R1865	T2076	T2074	arg3Of	),(
R1866	T1957	T1958	arg1Of	interleukin-6,(
R1867	T1959	T1958	arg2Of	IL-6,(
R1868	T2079	T2078	arg1Of	transfection,gene
R1869	T2079	T2080	arg1Of	transfection,or
R1870	T2080	T2077	arg2Of	or,Following
R1871	T1960	T1958	arg3Of	),(
R1872	T2082	T2080	arg2Of	transduction,or
R1873	T2082	T2081	arg1Of	transduction,protein
R1874	T2084	T2085	arg1Of	Tat,induced
R1875	T1962	T1961	arg2Of	34,(
R1876	T2084	T2099	arg1Of	Tat,increased
R1877	T2084	T2105	arg1Of	Tat,inhibiting
R1878	T2085	T2098	arg1Of	induced,and
R1879	T1963	T1961	arg3Of	),(
R1880	T2088	T2087	arg1Of	activity,IKK
R1881	T2088	T2089	arg1Of	activity,and
R1882	T1965	T1964	arg1Of	activate,to
R1883	T1967	T1965	arg2Of	transcription,activate
R1884	T2089	T2085	arg2Of	and,induced
R1885	T2089	T2086	arg1Of	and,the
R1886	T2089	T2092	arg1Of	and,of
R1887	T1967	T1966	arg1Of	transcription,gene
R1888	T2091	T2089	arg2Of	degradation,and
R1889	T2091	T2090	arg1Of	degradation,proteasomal
R1890	T1970	T1971	arg1Of	Tat,promotes
R1891	T2093	T2092	arg2Of	IκB-α,of
R1892	T2093	T2094	arg1Of	IκB-α,(
R1893	T2095	T2094	arg2Of	48,(
R1894	T2096	T2094	arg3Of	),(
R1895	T2098	T2077	arg1Of	and,Following
R1896	T2098	T2083	arg1Of	and,","
R1897	T2098	T2097	arg1Of	and,","
R1898	T2099	T2098	arg2Of	increased,and
R1899	T2099	T2104	arg1Of	increased,by
R1900	T1970	T1978	arg1Of	Tat,interacting
R1901	T1971	T1968	arg1Of	promotes,Indeed
R1902	T2103	T2099	arg2Of	activity,increased
R1903	T2103	T2100	arg1Of	activity,the
R1904	T2103	T2101	arg1Of	activity,p65
R1905	T2103	T2102	arg1Of	activity,transcriptional
R1906	T2105	T2104	arg2Of	inhibiting,by
R1907	T1971	T1969	arg1Of	promotes,","
R1908	T1971	T1977	arg1Of	promotes,by
R1909	T2108	T2105	arg2Of	deacetylation,inhibiting
R1910	T2108	T2106	arg1Of	deacetylation,the
R1911	T2108	T2107	arg1Of	deacetylation,SIRT-1-mediated
R1912	T2108	T2109	arg1Of	deacetylation,of
R1913	T1974	T1972	arg1Of	initiation,the
R1914	T2111	T2109	arg2Of	Lys310,of
R1915	T2111	T2110	arg1Of	Lys310,p65
R1916	T2111	T2112	arg1Of	Lys310,(
R1917	T2113	T2112	arg2Of	49,(
R1918	T2114	T2112	arg3Of	),(
R1919	T1974	T1973	arg1Of	initiation,transcriptional
R1920	T1974	T1975	arg1Of	initiation,and
R1921	T2116	T2115	arg1Of	findings,These
R1922	T2116	T2117	arg1Of	findings,suggested
R1923	T2117	T2127	arg1Of	suggested,;
R1924	T1975	T1971	arg2Of	and,promotes
R1925	T2119	T2120	arg1Of	Tat,modulates
R1926	T2120	T2117	arg2Of	modulates,suggested
R1927	T2120	T2118	arg1Of	modulates,that
R1928	T1976	T1975	arg2Of	elongation,and
R1929	T2122	T2120	arg2Of	enzymes,modulates
R1930	T2122	T2121	arg1Of	enzymes,crucial
R1931	T2122	T2123	arg2Of	enzymes,involved
R1932	T2123	T2124	arg1Of	involved,in
R1933	T1978	T1977	arg2Of	interacting,by
R1934	T2126	T2124	arg2Of	signaling,in
R1935	T2126	T2125	arg1Of	signaling,NF-κB
R1936	T1978	T1979	arg1Of	interacting,with
R1937	T1981	T1982	arg1Of	factors,and
R1938	T2131	T2127	arg2Of	was,;
R1939	T2131	T2128	arg1Of	was,however
R1940	T2131	T2129	arg1Of	was,","
R1941	T2132	T2131	arg2Of	unclear,was
R1942	T2133	T2130	arg1Of	how,it
R1943	T2133	T2131	arg1Of	how,was
R1944	T2133	T2132	arg1Of	how,unclear
R1945	T2134	T2135	arg1Of	Tat,could
R1946	T2134	T2136	arg1Of	Tat,subvert
R1947	T2136	T2133	arg1Of	subvert,how
R1948	T2136	T2135	arg2Of	subvert,could
R1949	T2139	T2136	arg2Of	feedback,subvert
R1950	T2139	T2137	arg1Of	feedback,the
R1951	T2139	T2138	arg1Of	feedback,negative
R1952	T2139	T2140	arg1Of	feedback,of
R1953	T2139	T2142	arg1Of	feedback,","
R1954	T2139	T2143	arg1Of	feedback,which
R1955	T2139	T2144	arg1Of	feedback,is
R1956	T2139	T2146	arg1Of	feedback,dependent
R1957	T2141	T2140	arg2Of	NF-κB,of
R1958	T1982	T1979	arg2Of	and,with
R1959	T1982	T1980	arg1Of	and,transacting
R1960	T2146	T2144	arg2Of	dependent,is
R1961	T2146	T2145	arg1Of	dependent,mainly
R1962	T2146	T2147	arg1Of	dependent,on
R1963	T1982	T1984	arg1Of	and,","
R1964	T1982	T1986	arg1Of	and,as
R1965	T1983	T1982	arg2Of	cofactors,and
R1966	T1986	T1985	arg1Of	as,such
R1967	T2149	T2148	arg1Of	novo,de
R1968	T2150	T2147	arg2Of	synthesis,on
R1969	T2150	T2149	arg1Of	synthesis,novo
R1970	T2150	T2151	arg1Of	synthesis,of
R1971	T2152	T2151	arg2Of	IκB-α,of
R1972	T2152	T2153	arg1Of	IκB-α,(
R1973	T1987	T1988	arg1Of	Sp1,(
R1974	T2154	T2153	arg2Of	"15,17",(
R1975	T2155	T2153	arg3Of	),(
R1976	T1987	T1991	arg1Of	Sp1,","
R1977	T2156	T2158	arg1Of	We,found
R1978	T1989	T1988	arg2Of	35,(
R1979	T2158	T2157	arg1Of	found,previously
R1980	T2160	T2161	arg1Of	IκB-α,binds
R1981	T2160	T2165	arg1Of	IκB-α,promotes
R1982	T1990	T1988	arg3Of	),(
R1983	T2161	T2162	arg1Of	binds,to
R1984	T2161	T2164	arg1Of	binds,and
R1985	T2163	T2162	arg2Of	Tat,to
R1986	T1991	T1996	arg1Of	",",","
R1987	T2164	T2158	arg2Of	and,found
R1988	T2164	T2159	arg1Of	and,that
R1989	T2165	T2164	arg2Of	promotes,and
R1990	T2187	T2184	arg2Of	turn,counteracts
R1991	T2168	T2165	arg2Of	export,promotes
R1992	T2168	T2166	arg1Of	export,the
R1993	T2168	T2167	arg1Of	export,nuclear
R1994	T2168	T2169	arg1Of	export,of
R1995	T2172	T2169	arg2Of	transactivator,of
R1996	T2172	T2170	arg1Of	transactivator,the
R1997	T2172	T2171	arg1Of	transactivator,viral
R1998	T2172	T2173	arg1Of	transactivator,(
R1999	T2174	T2173	arg2Of	"50,51",(
R2000	T2175	T2173	arg3Of	),(
R2001	T2178	T2176	arg2Of	study,In
R2002	T2178	T2177	arg1Of	study,this
R2003	T2187	T2185	arg1Of	turn,the
R2004	T2180	T2181	arg1Of	we,report
R2005	T2187	T2186	arg1Of	turn,post-activation
R2006	T2181	T2176	arg1Of	report,In
R2007	T2181	T2179	arg1Of	report,","
R2008	T2183	T2184	arg1Of	Tat,counteracts
R2009	T2188	T2189	arg1Of	off,of
R2010	T2184	T2181	arg2Of	counteracts,report
R2011	T2184	T2182	arg1Of	counteracts,that
R2012	T2184	T2188	arg1Of	counteracts,off
R2013	T2184	T2191	arg1Of	counteracts,through
R2014	T2190	T2189	arg2Of	NF-κB,of
R2015	T2193	T2191	arg2Of	interaction,through
R2016	T2193	T2192	arg1Of	interaction,direct
R2017	T2193	T2194	arg1Of	interaction,with
R2018	T2193	T2198	arg1Of	interaction,","
R2019	T2193	T2199	arg1Of	interaction,which
R2020	T2193	T2200	arg1Of	interaction,enhances
R2021	T2195	T2196	arg1Of	IκB-α,and
R2028	T2196	T2194	arg2Of	and,with
R2029	T2197	T2196	arg2Of	p65,and
R2033	T2203	T2202	arg1Of	binding,DNA
R2035	T2203	T2204	arg1Of	binding,and
R2036	T2205	T2204	arg2Of	transcriptional,and
R2037	T2206	T2200	arg2Of	activity,enhances
R2038	T2206	T2201	arg1Of	activity,the
R2039	T2206	T2203	arg1Of	activity,binding
R2040	T2206	T2205	arg1Of	activity,transcriptional
R2041	T2206	T2207	arg1Of	activity,of
R2042	T2210	T2207	arg2Of	complex,of
R2043	T2210	T2208	arg1Of	complex,the
R2044	T2210	T2209	arg1Of	complex,NF-κB
R2045	T2213	T2211	arg1Of	mechanism,The
R2046	T2213	T2212	arg1Of	mechanism,new
R2047	T2213	T2214	arg1Of	mechanism,of
R2048	T2213	T2219	arg1Of	mechanism,may
R2049	T2213	T2220	arg1Of	mechanism,provide
R2050	T2216	T2214	arg2Of	deregulation,of
R2051	T2216	T2215	arg1Of	deregulation,NF-κB
R2052	T2216	T2217	arg1Of	deregulation,here
R2053	T2216	T2218	arg2Of	deregulation,described
R2054	T2220	T2219	arg2Of	provide,may
R2055	T2220	T2223	arg1Of	provide,into
R2056	T2222	T2220	arg2Of	insights,provide
R2057	T2222	T2221	arg1Of	insights,further
R2058	T2227	T2223	arg2Of	activation,into
R2059	T2227	T2224	arg1Of	activation,the
R2060	T2227	T2225	arg1Of	activation,chronic
R2061	T2227	T2226	arg1Of	activation,immune
R2062	T2227	T2228	arg1Of	activation,of
R2063	T2230	T2228	arg2Of	infection,of
R2064	T2230	T2229	arg1Of	infection,HIV-1
R4014	T4416	T4417	arg1Of	pcDNA-3xHA-IκB-α,","
R4015	T4417	T4419	arg1Of	",",","
R4016	T4418	T4417	arg2Of	p3xFLAG-CMV-Tat,","
R4017	T4419	T4415	arg1Of	",",plasmids
R4018	T4419	T4426	arg1Of	",",","
R4019	T4423	T4422	arg2Of	"22,25,27",(
R4020	T4424	T4422	arg3Of	),(
R4021	T4425	T4419	arg2Of	A,","
R4022	T4425	T4420	arg1Of	A,p3xFLAG-CMV-Tat
R4023	T4425	T4421	arg1Of	A,C
R4024	T4425	T4422	arg1Of	A,(
R4025	T4426	T4433	arg1Of	",",","
R4026	T4430	T4429	arg2Of	"49,52,53,55,56,57",(
R4027	T4431	T4429	arg3Of	),(
R4028	T4432	T4426	arg2Of	A,","
R4029	T4432	T4427	arg1Of	A,p3xFLAG-CMV-Tat
R4030	T4432	T4428	arg1Of	A,R
R4031	T4432	T4429	arg1Of	A,(
R4032	T4433	T4435	arg1Of	",",","
R4033	T4434	T4433	arg2Of	pGEX-2T-Tat,","
R4034	T4435	T4442	arg1Of	",",and
R4035	T4439	T4438	arg2Of	"22,25,27",(
R4036	T4440	T4438	arg3Of	),(
R4037	T4441	T4435	arg2Of	A,","
R4038	T4441	T4436	arg1Of	A,pGEX-2T-Tat
R4039	T4441	T4437	arg1Of	A,C
R4040	T4441	T4438	arg1Of	A,(
R4041	T4442	T4414	arg1Of	and,The
R4042	T4442	T4449	arg1Of	and,were
R4043	T4442	T4451	arg2Of	and,described
R4044	T4446	T4445	arg2Of	"49,52,53,55,56,57",(
R4045	T4447	T4445	arg3Of	),(
R4046	T4448	T4442	arg2Of	A,and
R4047	T4448	T4443	arg1Of	A,pGEX-2T-Tat
R4048	T4448	T4444	arg1Of	A,R
R4049	T4448	T4445	arg1Of	A,(
R4050	T4451	T4449	arg2Of	described,were
R4051	T4451	T4450	arg1Of	described,previously
R4052	T4451	T4452	arg1Of	described,(
R4053	T4453	T4452	arg2Of	50,(
R4054	T4454	T4452	arg3Of	),(
R4055	T4457	T4458	arg1Of	pNL4-3.Luc.R-E-,and
R4056	T4458	T4455	arg1Of	and,The
R4057	T4458	T4456	arg1Of	and,plasmids
R4058	T4458	T4460	arg1Of	and,were
R4059	T4458	T4461	arg2Of	and,obtained
R4060	T4459	T4458	arg2Of	pHXB2-env,and
R4061	T4461	T4460	arg2Of	obtained,were
R4062	T4461	T4462	arg1Of	obtained,from
R4063	T4461	T4480	arg1Of	obtained,;
R4064	T4469	T4462	arg2Of	Program,from
R4065	T4469	T4463	arg1Of	Program,the
R4066	T4469	T4464	arg1Of	Program,AIDS
R4067	T4469	T4465	arg1Of	Program,Research
R4068	T4469	T4466	arg1Of	Program,&
R4069	T4469	T4467	arg1Of	Program,Reference
R4070	T4469	T4468	arg1Of	Program,Reagent
R4071	T4469	T4470	arg1Of	Program,","
R4072	T4469	T4478	arg1Of	Program,","
R4073	T4471	T4470	arg2Of	Division,","
R4074	T4471	T4472	arg1Of	Division,of
R4075	T4473	T4474	arg1Of	AIDS,","
R4076	T4474	T4476	arg1Of	",",","
R4077	T4475	T4474	arg2Of	NIAID,","
R4078	T4476	T4472	arg2Of	",",of
R4079	T4477	T4476	arg2Of	NIH,","
R4080	T4479	T4478	arg2Of	USA,","
R4081	T4481	T4482	arg1Of	pκBluc,and
R4082	T4482	T4484	arg1Of	and,were
R4083	T4482	T4485	arg2Of	and,purchased
R4084	T4483	T4482	arg2Of	pSV-β-Gal,and
R4085	T4485	T4480	arg2Of	purchased,;
R4086	T4485	T4484	arg2Of	purchased,were
R4087	T4485	T4486	arg1Of	purchased,from
R4088	T4487	T4486	arg2Of	Promega,from
R4089	T4487	T4488	arg1Of	Promega,(
R4090	T4489	T4490	arg1Of	Madison,","
R4091	T4490	T4488	arg2Of	",",(
R4092	T4490	T4492	arg1Of	",",","
R4093	T4491	T4490	arg2Of	WI,","
R4094	T4493	T4492	arg2Of	USA,","
R4095	T4494	T4488	arg3Of	),(
R4096	T4497	T4498	arg1Of	pRc/CMV-3xHA-p65,","
R4097	T4498	T4496	arg1Of	",",plasmids
R4098	T4498	T4503	arg1Of	",",","
R4099	T4499	T4498	arg2Of	pRc/CMV-3xHA-p65ΔC,","
R4100	T4499	T4500	arg1Of	pRc/CMV-3xHA-p65ΔC,(
R4101	T4501	T4500	arg2Of	1–318,(
R4102	T4502	T4500	arg3Of	),(
R4103	T4503	T4508	arg1Of	",",","
R4104	T4504	T4503	arg2Of	pRc/CMV-3xHA-p65ΔN,","
R4105	T4504	T4505	arg1Of	pRc/CMV-3xHA-p65ΔN,(
R4106	T4506	T4505	arg2Of	122–551,(
R4107	T4507	T4505	arg3Of	),(
R4108	T4508	T4511	arg1Of	",",","
R4109	T4510	T4508	arg2Of	T,","
R4110	T4510	T4509	arg1Of	T,p3xFLAG-CMV-Tat
R4111	T4511	T4517	arg1Of	",",","
R4112	T4514	T4513	arg2Of	"23,24",(
R4113	T4515	T4513	arg3Of	),(
R4114	T4516	T4511	arg2Of	A,","
R4115	T4516	T4512	arg1Of	A,N
R4116	T4516	T4513	arg1Of	A,(
R4117	T4517	T4495	arg1Of	",",The
R4118	T4517	T4542	arg1Of	",",were
R4119	T4517	T4543	arg2Of	",",generated
R4120	T4521	T4520	arg2Of	"50,51",(
R4121	T4522	T4520	arg3Of	),(
R4122	T4523	T4518	arg1Of	A,p3xFLAG-CMV-Tat
R4123	T4523	T4519	arg1Of	A,K
R4124	T4523	T4520	arg1Of	A,(
R4125	T4523	T4524	arg1Of	A,","
R4126	T4524	T4527	arg1Of	",",","
R4127	T4526	T4524	arg2Of	T,","
R4128	T4526	T4525	arg1Of	T,pGEX-2T-Tat
R4129	T4527	T4533	arg1Of	",",","
R4130	T4530	T4529	arg2Of	"23,24",(
R4131	T4531	T4529	arg3Of	),(
R4132	T4532	T4527	arg2Of	A,","
R4133	T4532	T4528	arg1Of	A,N
R4134	T4532	T4529	arg1Of	A,(
R4135	T4533	T4540	arg1Of	",",and
R4136	T4537	T4536	arg2Of	"50,51",(
R4137	T4538	T4536	arg3Of	),(
R4138	T4539	T4533	arg2Of	A,","
R4139	T4539	T4534	arg1Of	A,pGEX-2T-Tat
R4140	T4539	T4535	arg1Of	A,K
R4141	T4539	T4536	arg1Of	A,(
R4142	T4540	T4517	arg2Of	and,","
R4143	T4541	T4540	arg2Of	pNL4-3.FLAG-Tat.R-E-,and
R4144	T4543	T4542	arg2Of	generated,were
R4145	T4543	T4544	arg1Of	generated,as
R4146	T4545	T4544	arg2Of	described,as
R4147	T4545	T4546	arg1Of	described,in
R4148	T4548	T4546	arg2Of	Data,in
R4149	T4548	T4547	arg1Of	Data,Supplementary
R4308	T4915	T4916	arg1Of	Cells,","
R4309	T4916	T4918	arg1Of	",",","
R4310	T4917	T4916	arg2Of	transfection,","
R4311	T4918	T4920	arg1Of	",",and
R4312	T4919	T4918	arg2Of	treatments,","
R4313	T4922	T4920	arg2Of	assay,and
R4314	T4922	T4921	arg1Of	assay,luciferase
R4315	T4923	T4924	arg1Of	HeLa,","
R4316	T4925	T4924	arg2Of	p50−/−p65−/−,","
R4317	T4928	T4923	arg1Of	fibroblasts,HeLa
R4318	T4928	T4925	arg1Of	fibroblasts,p50−/−p65−/−
R4319	T4928	T4926	arg1Of	fibroblasts,mouse
R4320	T4928	T4927	arg1Of	fibroblasts,embryonic
R4321	T4928	T4929	arg1Of	fibroblasts,(
R4322	T4928	T4932	arg1Of	fibroblasts,(
R4323	T4928	T4935	arg1Of	fibroblasts,and
R4324	T4930	T4929	arg2Of	MEFs,(
R4325	T4931	T4929	arg3Of	),(
R4326	T4933	T4932	arg2Of	52,(
R4327	T4934	T4932	arg3Of	),(
R4328	T4935	T4938	arg1Of	and,were
R4329	T4935	T4939	arg2Of	and,cultured
R4330	T4937	T4935	arg2Of	cells,and
R4331	T4937	T4936	arg1Of	cells,293T
R4332	T4939	T4938	arg2Of	cultured,were
R4333	T4939	T4940	arg1Of	cultured,in
R4334	T4939	T4947	arg1Of	cultured,;
R4335	T4941	T4942	arg2Of	Dulbecco,'s
R4336	T4944	T4942	arg1Of	Eagle,'s
R4337	T4944	T4943	arg2Of	Eagle,modified
R4338	T4944	T4945	arg2Of	Eagle,'s
R4339	T4946	T4940	arg2Of	medium,in
R4340	T4946	T4945	arg1Of	medium,'s
R4341	T4948	T4949	arg1Of	Jurkat,","
R4342	T4949	T4952	arg1Of	",",and
R4343	T4951	T4949	arg2Of	cells,","
R4344	T4951	T4950	arg1Of	cells,U937
R4345	T4952	T4958	arg1Of	and,(
R4346	T4952	T4961	arg1Of	and,were
R4347	T4952	T4962	arg2Of	and,cultured
R4348	T4957	T4952	arg2Of	cells,and
R4349	T4957	T4953	arg1Of	cells,human
R4350	T4957	T4954	arg1Of	cells,peripheral
R4351	T4957	T4955	arg1Of	cells,blood
R4352	T4957	T4956	arg1Of	cells,mononuclear
R4353	T4959	T4958	arg2Of	PBMCs,(
R4354	T4960	T4958	arg3Of	),(
R4355	T4962	T4938	modOf	cultured,were
R4356	T4962	T4961	arg2Of	cultured,were
R4357	T4962	T4963	arg1Of	cultured,in
R4358	T4964	T4963	arg2Of	RPMI,in
R4359	T4964	T4965	arg1Of	RPMI,1640
R4360	T4966	T4967	arg1Of	PBMCs,were
R4361	T4966	T4968	arg2Of	PBMCs,isolated
R4362	T4968	T4967	arg2Of	isolated,were
R4363	T4968	T4969	arg1Of	isolated,as
R4364	T4971	T4969	arg2Of	described,as
R4365	T4971	T4970	arg1Of	described,previously
R4366	T4971	T4972	arg1Of	described,(
R4367	T4973	T4972	arg2Of	53,(
R4368	T4974	T4972	arg3Of	),(
R4369	T4975	T4976	arg1Of	Media,were
R4370	T4975	T4977	arg2Of	Media,supplemented
R4371	T4977	T4976	arg2Of	supplemented,were
R4372	T4977	T4978	arg1Of	supplemented,with
R4373	T4979	T4980	arg1Of	10,%
R4374	T4984	T4981	arg1Of	serum,heat-inactivated
R4375	T4984	T4982	arg1Of	serum,fetal
R4376	T4984	T4983	arg1Of	serum,calf
R4377	T4984	T4985	arg1Of	serum,and
R4378	T4985	T4978	arg2Of	and,with
R4379	T4985	T4979	arg1Of	and,10
R4380	T4985	T4988	arg1Of	and,(
R4381	T4987	T4985	arg2Of	l-glutamine,and
R4382	T4987	T4986	arg1Of	l-glutamine,2 mM
R4383	T4991	T4988	arg2Of	AG,(
R4384	T4991	T4989	arg1Of	AG,Lonza
R4385	T4991	T4990	arg1Of	AG,Cologne
R4386	T4991	T4992	arg1Of	AG,","
R4387	T4993	T4992	arg2Of	Germany,","
R4388	T4994	T4988	arg3Of	),(
R4389	T4995	T4996	arg1Of	HeLa,","
R4390	T4996	T4999	arg1Of	",",and
R4391	T4998	T4996	arg2Of	MEFs,","
R4392	T4998	T4997	arg1Of	MEFs,p50−/−p65−/−
R4393	T4999	T5001	arg1Of	and,were
R4394	T4999	T5002	arg2Of	and,transfected
R4395	T5000	T4999	arg2Of	293T,and
R4396	T5002	T5001	arg2Of	transfected,were
R4397	T5002	T5003	arg1Of	transfected,with
R4398	T5002	T5005	arg1Of	transfected,by
R4399	T5002	T5018	arg1Of	transfected,","
R4400	T5002	T5019	arg1Of	transfected,according
R4401	T5002	T5025	arg1Of	transfected,;
R4402	T5004	T5003	arg2Of	DNA,with
R4403	T5006	T5005	arg2Of	using,by
R4404	T5008	T5006	arg2Of	HD,using
R4405	T5008	T5007	arg1Of	HD,FuGENE
R4406	T5008	T5009	arg1Of	HD,(
R4407	T5012	T5009	arg2Of	GmbH,(
R4408	T5012	T5010	arg1Of	GmbH,Roche
R4409	T5012	T5011	arg1Of	GmbH,Diagnostic
R4410	T5012	T5013	arg1Of	GmbH,","
R4411	T5014	T5013	arg2Of	Mannheim,","
R4412	T5014	T5015	arg1Of	Mannheim,","
R4413	T5016	T5015	arg2Of	Germany,","
R4414	T5017	T5009	arg3Of	),(
R4415	T5020	T5019	arg2Of	to,according
R4416	T5023	T5020	arg2Of	's,to
R4417	T5023	T5021	arg1Of	's,the
R4418	T5023	T5022	arg1Of	's,manufacturer
R4419	T5023	T5024	arg1Of	's,protocol
R4420	T5028	T5026	arg1Of	amounts,total
R4421	T5028	T5027	arg1Of	amounts,DNA
R4422	T5028	T5029	arg1Of	amounts,were
R4423	T5028	T5030	arg2Of	amounts,equalized
R4424	T5030	T5025	arg2Of	equalized,;
R4425	T5030	T5029	arg2Of	equalized,were
R4426	T5032	T5030	arg1Of	transfection,equalized
R4427	T5032	T5031	arg2Of	transfection,by
R4428	T5032	T5033	arg1Of	transfection,of
R4429	T5036	T5033	arg2Of	vector,of
R4430	T5036	T5034	arg1Of	vector,pRc/CMV
R4431	T5036	T5035	arg1Of	vector,empty
R4432	T5036	T5037	arg1Of	vector,(
R4433	T5038	T5037	arg2Of	Invitrogen,(
R4434	T5038	T5039	arg1Of	Invitrogen,","
R4435	T5038	T5043	arg1Of	Invitrogen,","
R4436	T5038	T5044	arg1Of	Invitrogen,USA
R4437	T5040	T5041	arg1Of	Carlsbad,","
R4438	T5041	T5039	arg2Of	",",","
R4439	T5042	T5041	arg2Of	CA,","
R4440	T5045	T5037	arg3Of	),(
R4441	T5047	T5046	arg2Of	pulse-stimulation,For
R4442	T5050	T5049	arg1Of	cells,HeLa
R4443	T5050	T5051	arg1Of	cells,were
R4444	T5050	T5052	arg2Of	cells,treated
R4445	T5050	T5098	arg1Of	cells,returned
R4446	T5052	T5051	arg2Of	treated,were
R4447	T5052	T5053	arg1Of	treated,with
R4448	T5052	T5096	arg1Of	treated,and
R4449	T5056	T5054	arg1Of	13-acetate,phorbol
R4450	T5056	T5055	arg1Of	13-acetate,12-myristate
R4451	T5056	T5057	arg1Of	13-acetate,(
R4452	T5056	T5069	arg1Of	13-acetate,(
R4453	T5056	T5072	arg1Of	13-acetate,for
R4454	T5056	T5075	arg1Of	13-acetate,or
R4455	T5058	T5057	arg2Of	PMA,(
R4456	T5058	T5059	arg1Of	PMA,;
R4457	T5060	T5059	arg2Of	Sigma-Aldrich,;
R4458	T5060	T5061	arg1Of	Sigma-Aldrich,","
R4459	T5060	T5066	arg1Of	Sigma-Aldrich,","
R4460	T5060	T5067	arg1Of	Sigma-Aldrich,USA
R4461	T5063	T5061	arg2Of	Louis,","
R4462	T5063	T5062	arg1Of	Louis,St
R4463	T5063	T5064	arg1Of	Louis,","
R4464	T5065	T5064	arg2Of	MO,","
R4465	T5068	T5057	arg3Of	),(
R4466	T5070	T5069	arg2Of	20 ng/ml,(
R4467	T5071	T5069	arg3Of	),(
R4468	T5073	T5072	arg2Of	5 min,for
R4469	T5075	T5053	arg2Of	or,with
R4470	T5075	T5074	arg1Of	or,","
R4471	T5078	T5075	arg2Of	factor-α,or
R4472	T5078	T5076	arg1Of	factor-α,tumor
R4473	T5078	T5077	arg1Of	factor-α,necrosis
R4474	T5078	T5079	arg1Of	factor-α,(
R4475	T5078	T5084	arg1Of	factor-α,(
R4476	T5078	T5087	arg1Of	factor-α,for
R4477	T5078	T5089	arg1Of	factor-α,","
R4478	T5078	T5090	arg2Of	factor-α,washed
R4479	T5080	T5079	arg2Of	TNF-α,(
R4480	T5080	T5081	arg1Of	TNF-α,;
R4481	T5080	T5082	arg1Of	TNF-α,Sigma-Aldrich
R4482	T5083	T5079	arg3Of	),(
R4483	T5085	T5084	arg2Of	20 ng/ml,(
R4484	T5086	T5084	arg3Of	),(
R4485	T5088	T5087	arg2Of	30 min,for
R4486	T5090	T5091	arg1Of	washed,twice
R4487	T5090	T5092	arg1Of	washed,in
R4488	T5095	T5092	arg2Of	medium,in
R4489	T5095	T5093	arg1Of	medium,complete
R4490	T5095	T5094	arg1Of	medium,culture
R4491	T5096	T5046	arg1Of	and,For
R4492	T5096	T5048	arg1Of	and,","
R4493	T5098	T5096	arg2Of	returned,and
R4494	T5098	T5097	arg1Of	returned,then
R4495	T5098	T5099	arg1Of	returned,to
R4496	T5100	T5099	arg2Of	culture,to
R4497	T5103	T5101	arg2Of	assays,For
R4498	T5103	T5102	arg1Of	assays,luciferase
R4499	T5105	T5106	arg1Of	pSV-β-Gal,was
R4500	T5105	T5107	arg2Of	pSV-β-Gal,co-transfected
R4501	T5107	T5101	arg1Of	co-transfected,For
R4502	T5107	T5104	arg1Of	co-transfected,","
R4503	T5107	T5106	arg2Of	co-transfected,was
R4504	T5107	T5108	arg1Of	co-transfected,with
R4505	T5107	T5110	modOf	co-transfected,to
R4506	T5109	T5108	arg2Of	pκBluc,with
R4507	T5111	T5110	arg1Of	monitor,to
R4508	T5114	T5111	arg2Of	efficiency,monitor
R4509	T5114	T5112	arg1Of	efficiency,the
R4510	T5114	T5113	arg1Of	efficiency,transfection
R4511	T5118	T5115	arg1Of	cells,Forty-eight-hour
R4512	T5118	T5116	arg1Of	cells,post-transfection
R4513	T5118	T5117	arg1Of	cells,","
R4514	T5118	T5119	arg1Of	cells,were
R4515	T5118	T5120	arg2Of	cells,lysed
R4516	T5120	T5119	arg2Of	lysed,were
R4517	T5120	T5121	arg1Of	lysed,in
R4518	T5120	T5138	arg1Of	lysed,and
R4519	T5123	T5121	arg2Of	buffer,in
R4520	T5123	T5122	arg1Of	buffer,lysis
R4521	T5123	T5124	arg1Of	buffer,of
R4522	T5128	T5124	arg2Of	System,of
R4523	T5128	T5125	arg1Of	System,Dual
R4524	T5128	T5126	arg1Of	System,Light
R4525	T5128	T5127	arg1Of	System,Luciferase
R4526	T5128	T5129	arg1Of	System,(
R4527	T5130	T5131	arg1Of	Tropix,","
R4528	T5131	T5133	arg1Of	",",","
R4529	T5132	T5131	arg2Of	Bedford,","
R4530	T5133	T5129	arg2Of	",",(
R4531	T5133	T5135	arg1Of	",",","
R4532	T5134	T5133	arg2Of	MA,","
R4533	T5136	T5135	arg2Of	USA,","
R4534	T5137	T5129	arg3Of	),(
R4535	T5140	T5141	arg1Of	luciferase,and
R4536	T5142	T5141	arg2Of	β-galactosidase,and
R4537	T5143	T5139	arg1Of	activities,the
R4538	T5143	T5140	arg1Of	activities,luciferase
R4539	T5143	T5142	arg1Of	activities,β-galactosidase
R4540	T5143	T5144	arg1Of	activities,were
R4541	T5143	T5145	arg2Of	activities,evaluated
R4542	T5145	T5138	arg2Of	evaluated,and
R4543	T5145	T5144	arg2Of	evaluated,were
R4544	T5145	T5146	arg1Of	evaluated,by
R4545	T5147	T5146	arg2Of	using,by
R4546	T5147	T5155	arg1Of	using,in
R4547	T5147	T5158	arg1Of	using,(
R4548	T5151	T5147	arg2Of	System,using
R4549	T5151	T5148	arg1Of	System,Dual
R4550	T5151	T5149	arg1Of	System,Light
R4551	T5151	T5150	arg1Of	System,Luciferase
R4552	T5151	T5152	arg1Of	System,(
R4553	T5153	T5152	arg2Of	Tropix,(
R4554	T5154	T5152	arg3Of	),(
R4555	T5157	T5155	arg2Of	bioluminometer,in
R4556	T5157	T5156	arg1Of	bioluminometer,a
R4557	T5160	T5158	arg2Of	Biosystem,(
R4558	T5160	T5159	arg1Of	Biosystem,Turner
R4559	T5160	T5161	arg1Of	Biosystem,","
R4560	T5160	T5165	arg1Of	Biosystem,","
R4561	T5160	T5166	arg1Of	Biosystem,USA
R4562	T5162	T5161	arg2Of	Sunnyvale,","
R4563	T5162	T5163	arg1Of	Sunnyvale,","
R4564	T5164	T5163	arg2Of	CA,","
R4565	T5167	T5158	arg3Of	),(
R4566	T5169	T5168	arg1Of	ratio,The
R4567	T5169	T5170	arg1Of	ratio,of
R4568	T5169	T5174	arg1Of	ratio,to
R4569	T5169	T5177	arg1Of	ratio,was
R4570	T5169	T5178	arg2Of	ratio,expressed
R4571	T5173	T5170	arg2Of	activity,of
R4572	T5173	T5171	arg1Of	activity,firefly
R4573	T5173	T5172	arg1Of	activity,luciferase
R4574	T5176	T5174	arg2Of	activity,to
R4575	T5176	T5175	arg1Of	activity,β-galactosidase
R4576	T5178	T5177	arg2Of	expressed,was
R4577	T5178	T5179	arg1Of	expressed,as
R4578	T5182	T5179	arg2Of	units,as
R4579	T5182	T5180	arg1Of	units,relative
R4580	T5182	T5181	arg1Of	units,light
R4893	T5683	T5682	arg1Of	interference,RNA
R4894	T5684	T5685	arg1Of	Jurkat,or
R4895	T5686	T5685	arg2Of	U937,or
R4896	T5687	T5684	arg1Of	cells,Jurkat
R4897	T5687	T5686	arg1Of	cells,U937
R4898	T5687	T5688	arg1Of	cells,were
R4899	T5687	T5689	arg2Of	cells,transfected
R4900	T5689	T5688	arg2Of	transfected,were
R4901	T5691	T5689	arg1Of	electroporation,transfected
R4902	T5691	T5690	arg2Of	electroporation,by
R4903	T5691	T5692	arg1Of	electroporation,using
R4904	T5695	T5692	arg2Of	apparatus,using
R4905	T5695	T5693	arg1Of	apparatus,a
R4906	T5695	T5694	arg1Of	apparatus,Bio-Rad
R4907	T5695	T5696	arg1Of	apparatus,(
R4908	T5698	T5697	arg1Of	Laboratories,Bio-Rad
R4909	T5698	T5699	arg1Of	Laboratories,","
R4910	T5699	T5701	arg1Of	",",","
R4911	T5700	T5699	arg2Of	Hercules,","
R4912	T5701	T5696	arg2Of	",",(
R4913	T5701	T5703	arg1Of	",",","
R4914	T5701	T5704	arg1Of	",",USA
R4915	T5702	T5701	arg2Of	CA,","
R4916	T5705	T5696	arg3Of	),(
R4917	T5708	T5709	arg1Of	aliquots,(
R4918	T5708	T5713	arg1Of	aliquots,were
R4919	T5708	T5714	arg2Of	aliquots,suspended
R4920	T5708	T5728	arg2Of	aliquots,subjected
R4921	T5711	T5709	arg2Of	cells,(
R4922	T5711	T5710	arg1Of	cells,5 × 106
R4923	T5712	T5709	arg3Of	),(
R4924	T5714	T5715	arg1Of	suspended,in
R4925	T5714	T5727	arg1Of	suspended,and
R4926	T5716	T5715	arg2Of	0.3 ml,in
R4927	T5716	T5717	arg1Of	0.3 ml,of
R4928	T5716	T5720	arg2Of	0.3 ml,supplemented
R4929	T5718	T5717	arg2Of	RPMI,of
R4930	T5718	T5719	arg1Of	RPMI,1640
R4931	T5720	T5721	arg1Of	supplemented,with
R4932	T5722	T5723	arg1Of	20,%
R4933	T5726	T5721	arg2Of	serum,with
R4934	T5726	T5722	arg1Of	serum,20
R4935	T5726	T5724	arg1Of	serum,fetal
R4936	T5726	T5725	arg1Of	serum,calf
R4937	T5727	T5706	arg1Of	and,Briefly
R4938	T5727	T5707	arg1Of	and,","
R4939	T5727	T5713	arg2Of	and,were
R4940	T5727	T5748	arg1Of	and,;
R4941	T5728	T5727	arg2Of	subjected,and
R4942	T5728	T5729	arg1Of	subjected,to
R4943	T5728	T5739	arg1Of	subjected,in
R4944	T5733	T5729	arg2Of	pulse,to
R4945	T5733	T5730	arg1Of	pulse,a
R4946	T5733	T5731	arg1Of	pulse,double
R4947	T5733	T5732	arg1Of	pulse,electrical
R4948	T5733	T5734	arg1Of	pulse,(
R4949	T5735	T5734	arg2Of	0.22 V,(
R4950	T5735	T5736	arg1Of	0.22 V,","
R4951	T5737	T5736	arg2Of	960 µF,","
R4952	T5738	T5734	arg3Of	),(
R4953	T5739	T5740	arg1Of	in,the
R4954	T5739	T5742	arg1Of	in,of
R4955	T5741	T5739	arg2Of	presence,in
R4956	T5744	T5739	arg3Of	siRNA,in
R4957	T5744	T5743	arg2Of	siRNA,annealed
R4958	T5744	T5745	arg1Of	siRNA,(
R4959	T5746	T5745	arg2Of	200 pmol,(
R4960	T5747	T5745	arg3Of	),(
R4961	T5750	T5749	arg2Of	cells,electroporated
R4962	T5750	T5751	arg1Of	cells,were
R4963	T5750	T5752	arg2Of	cells,washed
R4964	T5750	T5754	arg2Of	cells,cultured
R4965	T5752	T5753	arg1Of	washed,and
R4966	T5753	T5748	arg2Of	and,;
R4967	T5753	T5751	arg2Of	and,were
R4968	T5753	T5755	arg1Of	and,in
R4969	T5754	T5753	arg2Of	cultured,and
R4970	T5757	T5755	arg2Of	medium,in
R4971	T5757	T5756	arg1Of	medium,complete
R4972	T5759	T5758	arg1Of	interference,RNA
R4973	T5759	T5760	arg1Of	interference,was
R4974	T5759	T5761	arg2Of	interference,performed
R4975	T5761	T5760	arg2Of	performed,was
R4976	T5761	T5762	arg1Of	performed,with
R4977	T5761	T5787	arg1Of	performed,UAGCAAUUGUGACAAGCAGUU
R4978	T5762	T5763	arg1Of	with,:
R4979	T5766	T5764	arg1Of	sense,siRNA
R4980	T5766	T5765	arg1Of	sense,Tat
R4981	T5766	T5767	arg1Of	sense,","
R4982	T5767	T5769	arg1Of	",",and
R4983	T5768	T5767	arg2Of	CUGCUUGUACCAAUUGCUAUU,","
R4984	T5771	T5769	arg2Of	Tat,and
R4985	T5771	T5770	arg1Of	Tat,siRNA
R4986	T5772	T5773	arg1Of	antisense,","
R4987	T5774	T5772	arg1Of	UAGCAAUUGGUACAAGCAGUU,antisense
R4988	T5774	T5775	arg1Of	UAGCAAUUGGUACAAGCAGUU,;
R4989	T5775	T5779	arg1Of	;,","
R4990	T5778	T5775	arg2Of	sense,;
R4991	T5778	T5776	arg1Of	sense,siRNA
R4992	T5778	T5777	arg1Of	sense,control
R4993	T5779	T5766	arg1Of	",",sense
R4994	T5779	T5768	arg1Of	",",CUGCUUGUACCAAUUGCUAUU
R4995	T5779	T5771	arg1Of	",",Tat
R4996	T5779	T5782	arg1Of	",",and
R4997	T5781	T5779	arg2Of	AUUGCUAUU,","
R4998	T5781	T5780	arg1Of	AUUGCUAUU,CUGCUUGUCACA
R4999	T5782	T5762	arg2Of	and,with
R5000	T5784	T5782	arg2Of	control,and
R5001	T5784	T5783	arg1Of	control,siRNA
R5002	T5785	T5786	arg1Of	antisense,","
R5003	T5787	T5785	arg1Of	UAGCAAUUGUGACAAGCAGUU,antisense
R5004	T5789	T5788	arg1Of	interference,RNA
R5005	T5789	T5790	arg1Of	interference,of
R5006	T5789	T5794	arg1Of	interference,was
R5007	T5789	T5795	arg2Of	interference,performed
R5008	T5791	T5792	arg1Of	p65,and
R5009	T5792	T5790	arg2Of	and,of
R5010	T5793	T5792	arg2Of	IκB-α,and
R5011	T5795	T5794	arg2Of	performed,was
R5012	T5795	T5796	arg1Of	performed,with
R5013	T5800	T5797	arg1Of	p65,SMART
R5014	T5800	T5798	arg1Of	p65,pool
R5015	T5800	T5799	arg1Of	p65,siRNA
R5016	T5800	T5801	arg1Of	p65,and
R5017	T5801	T5796	arg2Of	and,with
R5018	T5802	T5801	arg2Of	IκB-α,and
R5019	T5802	T5803	arg1Of	IκB-α,(
R5020	T5804	T5805	arg1Of	Dharmacon,","
R5021	T5805	T5807	arg1Of	",",","
R5022	T5806	T5805	arg2Of	Chicago,","
R5023	T5807	T5803	arg2Of	",",(
R5024	T5807	T5809	arg1Of	",",","
R5025	T5807	T5810	arg1Of	",",USA
R5026	T5808	T5807	arg2Of	IL,","
R5027	T5811	T5803	arg3Of	),(
R5180	T6050	T6049	arg2Of	virions,Pseudotyped
R5181	T6050	T6051	arg1Of	virions,and
R5182	T6054	T6051	arg2Of	infection,and
R5183	T6054	T6052	arg1Of	infection,single
R5184	T6054	T6053	arg1Of	infection,round
R5185	T6056	T6055	arg1Of	cells,293T
R5186	T6056	T6057	arg1Of	cells,(
R5187	T6056	T6060	arg1Of	cells,were
R5188	T6056	T6061	arg2Of	cells,transfected
R5189	T6058	T6057	arg2Of	1 × 107,(
R5190	T6059	T6057	arg3Of	),(
R5191	T6061	T6060	arg2Of	transfected,were
R5192	T6061	T6062	arg1Of	transfected,with
R5193	T6061	T6077	arg1Of	transfected,and
R5194	T6063	T6064	arg1Of	pNL4-3.Luc.R-E-,or
R5195	T6064	T6062	arg2Of	or,with
R5196	T6065	T6064	arg2Of	pNL4-3.FLAG-Tat.R-E-,or
R5197	T6065	T6066	arg1Of	pNL4-3.FLAG-Tat.R-E-,(
R5198	T6065	T6070	arg1Of	pNL4-3.FLAG-Tat.R-E-,with
R5199	T6067	T6066	arg2Of	10 µg,(
R5200	T6068	T6066	arg3Of	),(
R5201	T6070	T6069	arg1Of	with,together
R5202	T6072	T6070	arg2Of	Env,with
R5203	T6072	T6071	arg1Of	Env,pHXB2
R5204	T6072	T6073	arg1Of	Env,(
R5205	T6074	T6073	arg2Of	10 µg,(
R5206	T6075	T6073	arg3Of	),(
R5207	T6077	T6076	arg1Of	and,","
R5208	T6081	T6078	arg1Of	supernatant,48-h
R5209	T6081	T6079	arg1Of	supernatant,post-transfection
R5210	T6081	T6080	arg1Of	supernatant,cell
R5211	T6081	T6082	arg1Of	supernatant,was
R5212	T6081	T6083	arg2Of	supernatant,collected
R5213	T6083	T6077	arg2Of	collected,and
R5214	T6083	T6082	arg2Of	collected,was
R5215	T6086	T6084	arg1Of	assay,Enzyme-linked
R5216	T6086	T6085	arg1Of	assay,immunosorbent
R5217	T6086	T6087	arg1Of	assay,(
R5218	T6086	T6090	arg1Of	assay,using
R5219	T6088	T6087	arg2Of	ELISA,(
R5220	T6089	T6087	arg3Of	),(
R5221	T6095	T6090	arg2Of	concentration,using
R5222	T6095	T6091	arg1Of	concentration,anti-p24
R5223	T6095	T6092	arg1Of	concentration,antibody
R5224	T6095	T6093	arg2Of	concentration,measured
R5225	T6095	T6094	arg1Of	concentration,virion
R5226	T6096	T6097	arg1Of	PBMCs,","
R5227	T6097	T6099	arg1Of	",",or
R5228	T6098	T6097	arg2Of	Jurkat,","
R5229	T6099	T6102	arg1Of	or,(
R5230	T6099	T6105	arg1Of	or,were
R5231	T6099	T6106	arg2Of	or,infected
R5232	T6101	T6099	arg2Of	cells,or
R5233	T6101	T6100	arg1Of	cells,U937
R5234	T6103	T6102	arg2Of	5 × 107,(
R5235	T6104	T6102	arg3Of	),(
R5236	T6106	T6105	arg2Of	infected,were
R5237	T6106	T6107	arg1Of	infected,with
R5238	T6110	T6107	arg2Of	virions,with
R5239	T6110	T6108	arg1Of	virions,HXB2
R5240	T6110	T6109	arg1Of	virions,Env-pseudotyped
R5241	T6110	T6111	arg1Of	virions,(
R5242	T6110	T6116	arg1Of	virions,by
R5243	T6110	T6118	arg1Of	virions,","
R5244	T6110	T6119	arg1Of	virions,as
R5245	T6112	T6111	arg2Of	500 ng,(
R5246	T6112	T6113	arg1Of	500 ng,of
R5247	T6114	T6113	arg2Of	p24,of
R5248	T6115	T6111	arg3Of	),(
R5249	T6117	T6116	arg2Of	spinoculation,by
R5250	T6121	T6119	arg2Of	described,as
R5251	T6121	T6120	arg1Of	described,previously
R5252	T6121	T6122	arg1Of	described,(
R5253	T6123	T6122	arg2Of	50,(
R5254	T6124	T6122	arg3Of	),(
R5707	T6719	T6718	arg1Of	vitro,In
R5708	T6720	T6719	arg1Of	translation,vitro
R5709	T6721	T6722	arg1Of	HA-IκB-α,","
R5710	T6722	T6724	arg1Of	",",and
R5711	T6723	T6722	arg2Of	p65,","
R5712	T6724	T6726	arg1Of	and,were
R5713	T6724	T6727	arg2Of	and,expressed
R5714	T6724	T6735	arg2Of	and,translated
R5715	T6725	T6724	arg2Of	Tat,and
R5716	T6727	T6728	arg1Of	expressed,under
R5717	T6727	T6732	arg1Of	expressed,and
R5718	T6731	T6728	arg2Of	promoter,under
R5719	T6731	T6729	arg1Of	promoter,the
R5720	T6731	T6730	arg1Of	promoter,T7
R5721	T6732	T6726	arg2Of	and,were
R5722	T6734	T6733	arg1Of	vitro,in
R5723	T6735	T6732	arg2Of	translated,and
R5724	T6735	T6734	arg1Of	translated,vitro
R5725	T6735	T6736	modOf	translated,using
R5726	T6742	T6736	arg2Of	system,using
R5727	T6742	T6737	arg1Of	system,the
R5728	T6742	T6738	arg1Of	system,TnT
R5729	T6742	T6739	arg1Of	system,quick
R5730	T6742	T6740	arg2Of	system,coupled
R5731	T6742	T6741	arg1Of	system,transcription/translation
R5732	T6742	T6743	arg1Of	system,(
R5733	T6742	T6746	arg1Of	system,","
R5734	T6742	T6747	arg1Of	system,as
R5735	T6744	T6743	arg2Of	Promega,(
R5736	T6745	T6743	arg3Of	),(
R5737	T6749	T6747	arg2Of	reported,as
R5738	T6749	T6748	arg1Of	reported,previously
R5739	T6749	T6750	arg1Of	reported,(
R5740	T6751	T6750	arg2Of	50,(
R5741	T6752	T6750	arg3Of	),(
R5742	T6753	T6754	arg1Of	Details,are
R5743	T6753	T6755	arg2Of	Details,described
R5744	T6755	T6754	arg2Of	described,are
R5745	T6755	T6756	arg1Of	described,in
R5746	T6758	T6756	arg2Of	Data,in
R5747	T6758	T6757	arg1Of	Data,Supplementary
R5827	T6883	T6882	arg1Of	assay,Immunoprecipitation
R5828	T6883	T6884	arg1Of	assay,and
R5829	T6885	T6884	arg2Of	GST-pull,and
R5830	T6885	T6886	arg1Of	GST-pull,down
R5831	T6887	T6888	arg1Of	Immunoprecipitation,","
R5832	T6888	T6890	arg1Of	",",down
R5833	T6888	T6892	arg1Of	",",and
R5834	T6889	T6888	arg2Of	GST-pull,","
R5835	T6892	T6891	arg1Of	and,","
R5836	T6892	T6894	arg1Of	and,of
R5837	T6892	T6897	arg1Of	and,in
R5838	T6892	T6902	arg1Of	and,were
R5839	T6892	T6903	arg2Of	and,performed
R5840	T6893	T6892	arg2Of	production,and
R5841	T6896	T6894	arg2Of	proteins,of
R5842	T6896	T6895	arg1Of	proteins,GST
R5843	T6901	T6897	arg2Of	BL21,in
R5844	T6901	T6898	arg1Of	BL21,Escherichia
R5845	T6901	T6899	arg1Of	BL21,coli
R5846	T6901	T6900	arg1Of	BL21,strain
R5847	T6903	T6902	arg2Of	performed,were
R5848	T6903	T6904	arg1Of	performed,as
R5849	T6906	T6904	arg2Of	reported,as
R5850	T6906	T6905	arg1Of	reported,previously
R5851	T6906	T6907	arg1Of	reported,(
R5899	T7017	T7016	arg1Of	PCR,Real-time
R5900	T7019	T7018	arg1Of	RNA,Total
R5901	T7019	T7020	arg1Of	RNA,was
R5902	T7019	T7021	arg2Of	RNA,extracted
R5903	T7019	T7025	arg1Of	RNA,using
R5904	T7021	T7020	arg2Of	extracted,was
R5905	T7021	T7022	arg1Of	extracted,from
R5906	T7021	T7024	arg1Of	extracted,by
R5907	T7021	T7032	arg1Of	extracted,;
R5908	T7023	T7022	arg2Of	cells,from
R5909	T7025	T7024	arg2Of	using,by
R5910	T7028	T7025	arg2Of	reagent,using
R5911	T7028	T7026	arg1Of	reagent,the
R5912	T7028	T7027	arg1Of	reagent,TRIzol
R5913	T7028	T7029	arg1Of	reagent,(
R5914	T7030	T7029	arg2Of	Invitrogen,(
R5915	T7031	T7029	arg3Of	),(
R5916	T7034	T7033	arg1Of	aliquots,RNA
R5917	T7034	T7035	arg1Of	aliquots,(
R5918	T7034	T7038	arg1Of	aliquots,were
R5919	T7036	T7035	arg2Of	200 ng,(
R5920	T7037	T7035	arg3Of	),(
R5921	T7038	T7032	arg2Of	were,;
R5922	T7038	T7055	arg1Of	were,","
R5923	T7038	T7056	arg1Of	were,according
R5924	T7043	T7039	arg1Of	Examers,reverse
R5925	T7043	T7040	arg2Of	Examers,transcribed
R5926	T7043	T7041	arg1Of	Examers,using
R5927	T7043	T7042	arg1Of	Examers,Random
R5928	T7043	T7044	arg1Of	Examers,(
R5929	T7043	T7047	arg1Of	Examers,and
R5930	T7045	T7044	arg2Of	Roche,(
R5931	T7046	T7044	arg3Of	),(
R5932	T7047	T7038	arg2Of	and,were
R5933	T7051	T7047	arg2Of	Transcriptase,and
R5934	T7051	T7048	arg1Of	Transcriptase,Superscript
R5935	T7051	T7049	arg1Of	Transcriptase,III
R5936	T7051	T7050	arg1Of	Transcriptase,Reverse
R5937	T7051	T7052	arg1Of	Transcriptase,(
R5938	T7053	T7052	arg2Of	Invitrogen,(
R5939	T7054	T7052	arg3Of	),(
R5940	T7057	T7056	arg2Of	to,according
R5941	T7059	T7058	arg1Of	manufacturer,the
R5942	T7059	T7060	arg2Of	manufacturer,'s
R5943	T7061	T7057	arg2Of	protocol,to
R5944	T7061	T7060	arg1Of	protocol,'s
R5945	T7063	T7062	arg1Of	PCR,Real-time
R5946	T7063	T7064	arg1Of	PCR,was
R5947	T7063	T7065	arg2Of	PCR,performed
R5948	T7063	T7077	arg1Of	PCR,carried
R5949	T7065	T7064	arg2Of	performed,was
R5950	T7065	T7066	arg1Of	performed,with
R5951	T7065	T7076	arg1Of	performed,and
R5952	T7070	T7069	arg1Of	Super,Green
R5953	T7071	T7066	arg2Of	mix,with
R5954	T7071	T7067	arg1Of	mix,the
R5955	T7071	T7068	arg1Of	mix,iQ
R5956	T7071	T7070	arg1Of	mix,Super
R5957	T7071	T7072	arg1Of	mix,(
R5958	T7074	T7072	arg2Of	Laboratories,(
R5959	T7074	T7073	arg1Of	Laboratories,Bio-Rad
R5960	T7075	T7072	arg3Of	),(
R5961	T7076	T7094	arg1Of	and,:
R5962	T7077	T7076	arg2Of	carried,and
R5963	T7077	T7078	arg1Of	carried,out
R5964	T7077	T7079	arg1Of	carried,with
R5965	T7085	T7079	arg2Of	system,with
R5966	T7085	T7080	arg1Of	system,the
R5967	T7085	T7081	arg1Of	system,iCycler
R5968	T7085	T7082	arg1Of	system,iQ
R5969	T7085	T7083	arg1Of	system,Real-Time
R5970	T7085	T7084	arg1Of	system,detection
R5971	T7085	T7086	arg1Of	system,(
R5972	T7085	T7090	arg1Of	system,under
R5973	T7088	T7086	arg2Of	Laboratories,(
R5974	T7088	T7087	arg1Of	Laboratories,Bio-Rad
R5975	T7089	T7086	arg3Of	),(
R5976	T7093	T7090	arg2Of	conditions,under
R5977	T7093	T7091	arg1Of	conditions,the
R5978	T7093	T7092	arg1Of	conditions,following
R5979	T7095	T7096	arg1Of	95°C,","
R5980	T7095	T7098	arg1Of	95°C,;
R5981	T7097	T7096	arg2Of	1 min,","
R5982	T7100	T7099	arg2Of	94°C,(
R5983	T7100	T7101	arg1Of	94°C,","
R5984	T7100	T7103	arg1Of	94°C,;
R5985	T7102	T7101	arg2Of	10 s,","
R5986	T7104	T7103	arg2Of	60°C,;
R5987	T7104	T7105	arg1Of	60°C,","
R5988	T7106	T7105	arg2Of	30 s,","
R5989	T7107	T7099	arg3Of	),(
R5990	T7108	T7098	arg2Of	×40,;
R5991	T7108	T7099	arg1Of	×40,(
R5992	T7109	T7110	arg1Of	Primers,for
R5993	T7109	T7114	arg1Of	Primers,are
R5994	T7109	T7115	arg2Of	Primers,listed
R5995	T7111	T7112	arg1Of	Tat,and
R5996	T7112	T7110	arg2Of	and,for
R5997	T7113	T7112	arg2Of	MIP-1α,and
R5998	T7115	T7114	arg2Of	listed,are
R5999	T7115	T7116	arg1Of	listed,in
R6000	T7119	T7117	arg1Of	Real-time,Supplementary
R6001	T7119	T7118	arg1Of	Real-time,Data.
R6002	T7120	T7116	arg2Of	PCR,in
R6003	T7120	T7119	arg1Of	PCR,Real-time
R6004	T7120	T7121	arg1Of	PCR,of
R6005	T7122	T7121	arg2Of	CSF3,of
R6006	T7124	T7125	arg1Of	LTA,","
R6007	T7125	T7127	arg1Of	",",","
R6008	T7126	T7125	arg2Of	NFKBIA,","
R6009	T7127	T7129	arg1Of	",",","
R6010	T7128	T7127	arg2Of	TLR2,","
R6011	T7129	T7131	arg1Of	",",and
R6012	T7130	T7129	arg2Of	GAPDH,","
R6013	T7131	T7133	arg1Of	and,was
R6014	T7131	T7134	arg2Of	and,performed
R6015	T7132	T7131	arg2Of	ACTB,and
R6016	T7134	T7114	modOf	performed,are
R6017	T7134	T7123	arg1Of	performed,","
R6018	T7134	T7133	arg2Of	performed,was
R6019	T7135	T7134	arg3Of	using,performed
R6020	T7143	T7135	arg2Of	pathway,using
R6021	T7143	T7136	arg1Of	pathway,the
R6022	T7143	T7137	arg1Of	pathway,RT2
R6023	T7143	T7138	arg1Of	pathway,profiler
R6024	T7143	T7139	arg1Of	pathway,PCR
R6025	T7143	T7140	arg1Of	pathway,Array-Human
R6026	T7143	T7141	arg1Of	pathway,NF-κB
R6027	T7143	T7142	arg1Of	pathway,signaling
R6028	T7143	T7144	arg1Of	pathway,(
R6029	T7146	T7144	arg2Of	Sciences,(
R6030	T7146	T7145	arg1Of	Sciences,QIAGEN
R6031	T7146	T7147	arg1Of	Sciences,","
R6032	T7148	T7147	arg2Of	MD,","
R6033	T7148	T7149	arg1Of	MD,","
R6034	T7150	T7149	arg2Of	USA,","
R6035	T7151	T7144	arg3Of	),(
R6036	T7152	T7153	arg1Of	Reactions,were
R6037	T7152	T7154	arg2Of	Reactions,carried
R6038	T7154	T7153	arg2Of	carried,were
R6039	T7154	T7155	arg1Of	carried,out
R6040	T7154	T7156	arg1Of	carried,in
R6041	T7154	T7159	arg1Of	carried,and
R6042	T7157	T7156	arg2Of	triplicate,in
R6043	T7159	T7158	arg1Of	and,","
R6044	T7162	T7160	arg1Of	levels,gene
R6045	T7162	T7161	arg1Of	levels,expression
R6046	T7162	T7163	arg1Of	levels,were
R6047	T7162	T7164	arg2Of	levels,calculated
R6048	T7164	T7159	arg2Of	calculated,and
R6049	T7164	T7163	arg2Of	calculated,were
R6050	T7164	T7166	arg1Of	calculated,to
R6051	T7164	T7170	arg1Of	calculated,as
R6052	T7166	T7165	arg1Of	to,relative
R6053	T7169	T7166	arg2Of	levels,to
R6054	T7169	T7167	arg1Of	levels,GAPDH
R6055	T7169	T7168	arg1Of	levels,mRNA
R6056	T7172	T7170	arg2Of	control,as
R6057	T7172	T7171	arg1Of	control,endogenous
R6058	T7174	T7173	arg1Of	expression,Relative
R6059	T7174	T7175	arg1Of	expression,was
R6060	T7174	T7176	arg2Of	expression,calculated
R6061	T7176	T7175	arg2Of	calculated,was
R6062	T7176	T7177	arg1Of	calculated,as
R6063	T7178	T7177	arg2Of	2,as
R6064	T7178	T7179	arg1Of	2,(
R6065	T7181	T7179	arg2Of	gene,(
R6066	T7181	T7180	arg1Of	gene,Ct
R6067	T7181	T7182	arg1Of	gene,under
R6068	T7184	T7182	arg2Of	GAPDH,under
R6069	T7184	T7183	arg1Of	GAPDH,investigation − Ct
R6070	T7185	T7179	arg3Of	),(
R6779	T8160	T8159	arg1Of	analysis,Statistical
R6780	T8162	T8161	arg1Of	analysis,Statistical
R6781	T8162	T8163	arg1Of	analysis,was
R6782	T8162	T8164	arg2Of	analysis,performed
R6783	T8164	T8163	arg2Of	performed,was
R6784	T8168	T8166	arg1Of	Student,two-tail
R6785	T8168	T8167	arg1Of	Student,unpaired
R6786	T8168	T8169	arg2Of	Student,'s
R6787	T8170	T8164	arg1Of	t-test,performed
R6788	T8170	T8165	arg2Of	t-test,by
R6789	T8170	T8169	arg1Of	t-test,'s
R6790	T8171	T8172	arg1Of	Data,were
R6791	T8171	T8173	arg2Of	Data,reported
R6792	T8173	T8172	arg2Of	reported,were
R6793	T8173	T8174	arg1Of	reported,as
R6794	T8175	T8174	arg2Of	means ± SE,as
R6795	T8176	T8177	arg1Of	Differences,between
R6796	T8176	T8180	arg1Of	Differences,were
R6797	T8176	T8181	arg2Of	Differences,considered
R6798	T8176	T8184	arg1Of	Differences,significant
R6799	T8179	T8177	arg2Of	means,between
R6800	T8179	T8178	arg1Of	means,the
R6801	T8181	T8180	arg2Of	considered,were
R6802	T8181	T8190	arg1Of	considered,(
R6803	T8184	T8181	arg3Of	significant,considered
R6804	T8184	T8182	arg1Of	significant,as
R6805	T8184	T8183	arg1Of	significant,statistically
R6806	T8184	T8185	arg1Of	significant,at
R6807	T8187	T8188	arg1Of	95,%
R6808	T8189	T8185	arg2Of	level,at
R6809	T8189	T8186	arg1Of	level,the
R6810	T8189	T8187	arg1Of	level,95
R6811	T8191	T8190	arg2Of	P ,(
R6812	T8191	T8192	arg1Of	P ,<
R6813	T8192	T8193	arg1Of	<, 0.05
R6814	T8194	T8190	arg3Of	),(
R7042	T8554	T8555	arg1Of	Tat,enhances
R7043	T8554	T8560	arg1Of	Tat,hijacking
R7044	T8554	T8563	arg1Of	Tat,inhibiting
R7045	T8555	T8559	arg1Of	enhances,by
R7046	T8558	T8555	arg2Of	activity,enhances
R7047	T8558	T8556	arg1Of	activity,the
R7048	T8558	T8557	arg1Of	activity,NF-κB
R7049	T8560	T8562	arg1Of	hijacking,and
R7050	T8561	T8560	arg2Of	IκB-α,hijacking
R7051	T8562	T8559	arg2Of	and,by
R7052	T8563	T8562	arg2Of	inhibiting,and
R7053	T8563	T8567	arg1Of	inhibiting,off
R7054	T8566	T8563	arg2Of	turn,inhibiting
R7055	T8566	T8564	arg1Of	turn,the
R7056	T8566	T8565	arg1Of	turn,post-activation
R7057	T8567	T8568	arg1Of	off,of
R7058	T8569	T8568	arg2Of	NF-κB,of
R7059	T8570	T8571	arg1Of	We,analysed
R7060	T8570	T8583	arg1Of	We,modulating
R7061	T8571	T8582	arg1Of	analysed,by
R7062	T8573	T8571	arg2Of	kinetic,analysed
R7063	T8573	T8572	arg1Of	kinetic,the
R7064	T8573	T8574	arg1Of	kinetic,of
R7065	T8576	T8574	arg2Of	activation,of
R7066	T8576	T8575	arg1Of	activation,NF-κB
R7067	T8576	T8577	arg1Of	activation,in
R7068	T8581	T8577	arg2Of	infection,in
R7069	T8581	T8578	arg1Of	infection,single
R7070	T8581	T8579	arg1Of	infection,round
R7071	T8581	T8580	arg1Of	infection,HIV-1
R7072	T8583	T8582	arg2Of	modulating,by
R7073	T8585	T8583	arg2Of	expression,modulating
R7074	T8585	T8584	arg1Of	expression,the
R7075	T8585	T8586	arg1Of	expression,of
R7076	T8587	T8586	arg2Of	Tat,of
R7077	T8587	T8588	arg1Of	Tat,with
R7078	T8590	T8588	arg2Of	interference,with
R7079	T8590	T8589	arg1Of	interference,RNA
R7080	T8592	T8591	arg1Of	cells,Jurkat
R7081	T8592	T8593	arg1Of	cells,were
R7082	T8592	T8594	arg2Of	cells,transfected
R7083	T8592	T8603	arg1Of	cells,left
R7084	T8594	T8593	arg2Of	transfected,were
R7085	T8594	T8595	arg1Of	transfected,with
R7086	T8594	T8602	arg1Of	transfected,or
R7087	T8597	T8596	arg1Of	Tat,siRNA
R7088	T8597	T8598	arg1Of	Tat,","
R7089	T8598	T8595	arg2Of	",",with
R7090	T8600	T8598	arg2Of	control,","
R7091	T8600	T8599	arg1Of	control,siRNA
R7092	T8602	T8601	arg1Of	or,","
R7093	T8602	T8606	arg1Of	or,and
R7094	T8603	T8602	arg2Of	left,or
R7095	T8604	T8603	arg2Of	untransfected,left
R7096	T8606	T8605	arg1Of	and,","
R7097	T8609	T8607	arg1Of	cells,24 h
R7098	T8609	T8608	arg1Of	cells,later
R7099	T8609	T8610	arg1Of	cells,were
R7100	T8609	T8611	arg2Of	cells,infected
R7101	T8611	T8606	arg2Of	infected,and
R7102	T8611	T8610	arg2Of	infected,were
R7103	T8611	T8612	arg1Of	infected,with
R7104	T8616	T8612	arg2Of	virions,with
R7105	T8616	T8613	arg1Of	virions,HXB2
R7106	T8616	T8614	arg1Of	virions,Env-pseudotyped
R7107	T8616	T8615	arg1Of	virions,NL4-3.Luc.R−E−
R7108	T8619	T8617	arg2Of	analysis,By
R7109	T8619	T8618	arg1Of	analysis,cytofluorimetric
R7110	T8622	T8621	arg1Of	%,∼40
R7111	T8622	T8623	arg1Of	%,of
R7112	T8622	T8625	arg1Of	%,was
R7113	T8622	T8626	arg1Of	%,siRNA-transfected
R7114	T8622	T8628	arg1Of	%,HIV-1-infected
R7115	T8624	T8623	arg2Of	cells,of
R7116	T8625	T8617	arg1Of	was,By
R7117	T8625	T8620	arg1Of	was,","
R7118	T8625	T8629	arg1Of	was,at
R7119	T8626	T8627	arg1Of	siRNA-transfected,and
R7120	T8627	T8625	arg2Of	and,was
R7121	T8628	T8627	arg2Of	HIV-1-infected,and
R7122	T8631	T8629	arg2Of	post-infection,at
R7123	T8631	T8630	arg1Of	post-infection,12-h
R7124	T8631	T8632	arg1Of	post-infection,(
R7125	T8635	T8632	arg2Of	S1,(
R7126	T8635	T8633	arg1Of	S1,Supplementary
R7127	T8635	T8634	arg1Of	S1,Figure
R7128	T8636	T8632	arg3Of	),(
R7129	T8638	T8637	arg1Of	expression,The
R7130	T8638	T8639	arg1Of	expression,of
R7131	T8638	T8641	arg1Of	expression,was
R7132	T8638	T8642	arg2Of	expression,detected
R7133	T8638	T8648	arg1Of	expression,increased
R7134	T8638	T8651	arg1Of	expression,12 h
R7135	T8640	T8639	arg2Of	Tat,of
R7136	T8642	T8641	arg2Of	detected,was
R7137	T8642	T8643	arg1Of	detected,at
R7138	T8642	T8646	arg1Of	detected,and
R7139	T8645	T8643	arg2Of	post-infection,at
R7140	T8645	T8644	arg1Of	post-infection,1-h
R7141	T8648	T8646	arg2Of	increased,and
R7142	T8648	T8647	arg1Of	increased,progressively
R7143	T8648	T8649	arg1Of	increased,up
R7144	T8648	T8650	modOf	increased,to
R7145	T8648	T8658	arg1Of	increased,","
R7146	T8648	T8659	arg1Of	increased,while
R7147	T8651	T8650	arg1Of	12 h,to
R7148	T8651	T8652	arg1Of	12 h,in
R7149	T8653	T8654	arg1Of	untransfected,and
R7150	T8656	T8654	arg2Of	control-transfected,and
R7151	T8656	T8655	arg1Of	control-transfected,siRNA
R7152	T8657	T8652	arg2Of	cells,in
R7153	T8657	T8653	arg1Of	cells,untransfected
R7154	T8657	T8656	arg1Of	cells,control-transfected
R7155	T8660	T8661	arg1Of	it,was
R7156	T8660	T8663	arg2Of	it,detected
R7157	T8663	T8659	arg2Of	detected,while
R7158	T8663	T8661	arg2Of	detected,was
R7159	T8663	T8662	arg1Of	detected,barely
R7160	T8663	T8664	arg1Of	detected,in
R7161	T8667	T8664	arg2Of	cells,in
R7162	T8667	T8665	arg1Of	cells,siRNA
R7163	T8667	T8666	arg1Of	cells,Tat-transfected
R7164	T8667	T8668	arg1Of	cells,(
R7165	T8670	T8668	arg2Of	1A,(
R7166	T8670	T8669	arg1Of	1A,Figure
R7167	T8671	T8668	arg3Of	),(
R7168	T8675	T8672	arg1Of	expression,The
R7169	T8675	T8673	arg1Of	expression,LTR-dependent
R7170	T8675	T8674	arg1Of	expression,luciferase
R7171	T8675	T8676	arg1Of	expression,was
R7172	T8675	T8679	arg2Of	expression,induced
R7173	T8679	T8676	arg2Of	induced,was
R7174	T8679	T8677	arg1Of	induced,also
R7175	T8679	T8678	arg1Of	induced,progressively
R7176	T8679	T8680	arg1Of	induced,at
R7177	T8679	T8685	arg1Of	induced,in
R7178	T8679	T8691	arg1Of	induced,","
R7179	T8679	T8692	arg1Of	induced,while
R7180	T8683	T8681	arg1Of	12-h,1-
R7181	T8683	T8682	arg1Of	12-h,to
R7182	T8684	T8680	arg2Of	post-infection,at
R7183	T8684	T8683	arg1Of	post-infection,12-h
R7184	T8686	T8687	arg1Of	untransfected,and
R7185	T8689	T8687	arg2Of	control-transfected,and
R7186	T8689	T8688	arg1Of	control-transfected,siRNA
R7187	T8690	T8685	arg2Of	cells,in
R7188	T8690	T8686	arg1Of	cells,untransfected
R7189	T8690	T8689	arg1Of	cells,control-transfected
R7190	T8693	T8694	arg1Of	it,was
R7191	T8693	T8696	arg2Of	it,observed
R7192	T8696	T8692	arg2Of	observed,while
R7193	T8696	T8694	arg2Of	observed,was
R7194	T8696	T8695	arg1Of	observed,barely
R7195	T8696	T8697	arg1Of	observed,in
R7196	T8696	T8701	arg1Of	observed,","
R7197	T8700	T8697	arg2Of	cells,in
R7198	T8700	T8698	arg1Of	cells,siRNA
R7199	T8700	T8699	arg1Of	cells,Tat-transfected
R7200	T8702	T8696	arg3Of	as,observed
R7201	T8703	T8702	arg2Of	expected,as
R7202	T8703	T8704	arg1Of	expected,for
R7203	T8707	T8704	arg2Of	transactivation,for
R7204	T8707	T8705	arg1Of	transactivation,the
R7205	T8707	T8706	arg1Of	transactivation,Tat-dependent
R7206	T8707	T8708	arg1Of	transactivation,of
R7207	T8711	T8708	arg2Of	LTR,of
R7208	T8711	T8709	arg1Of	LTR,the
R7209	T8711	T8710	arg1Of	LTR,HIV-1
R7210	T8711	T8712	arg1Of	LTR,(
R7211	T8714	T8712	arg2Of	1A,(
R7212	T8714	T8713	arg1Of	1A,Figure
R7213	T8715	T8712	arg3Of	),(
R7214	T8718	T8716	arg1Of	increase,The
R7215	T8718	T8717	arg1Of	increase,progressive
R7216	T8718	T8719	arg1Of	increase,in
R7217	T8718	T8747	arg1Of	increase,was
R7218	T8718	T8748	arg2Of	increase,observed
R7219	T8721	T8719	arg2Of	activity,in
R7220	T8721	T8720	arg1Of	activity,NF-κB
R7221	T8724	T8723	arg2Of	measured,as
R7222	T8727	T8724	arg1Of	binding,measured
R7223	T8727	T8725	arg2Of	binding,by
R7224	T8727	T8726	arg1Of	binding,p65–p50
R7225	T8727	T8728	arg1Of	binding,to
R7226	T8732	T8729	arg1Of	oligonucleotide,an
R7227	T8732	T8730	arg1Of	oligonucleotide,NF-κB
R7228	T8732	T8731	arg1Of	oligonucleotide,consensus
R7229	T8732	T8733	arg1Of	oligonucleotide,(
R7230	T8732	T8737	arg1Of	oligonucleotide,and
R7231	T8735	T8733	arg2Of	1B,(
R7232	T8735	T8734	arg1Of	1B,Figure
R7233	T8736	T8733	arg3Of	),(
R7234	T8737	T8728	arg2Of	and,to
R7235	T8739	T8737	arg2Of	p65,and
R7236	T8739	T8738	arg1Of	p65,nuclear
R7237	T8739	T8740	arg1Of	p65,(
R7238	T8742	T8740	arg2Of	1C,(
R7239	T8742	T8741	arg1Of	1C,Figure
R7240	T8742	T8743	arg1Of	1C,","
R7241	T8744	T8743	arg2Of	nucleus,","
R7242	T8745	T8740	arg3Of	),(
R7243	T8748	T8722	arg1Of	observed,","
R7244	T8748	T8723	arg1Of	observed,as
R7245	T8748	T8746	arg1Of	observed,","
R7246	T8748	T8747	arg2Of	observed,was
R7247	T8748	T8749	arg1Of	observed,at
R7248	T8748	T8754	arg1Of	observed,in
R7249	T8748	T8771	arg1Of	observed,;
R7250	T8752	T8750	arg1Of	3-h,1-
R7251	T8752	T8751	arg1Of	3-h,to
R7252	T8753	T8749	arg2Of	post-infection,at
R7253	T8753	T8752	arg1Of	post-infection,3-h
R7254	T8756	T8755	arg1Of	Tat-positive,both
R7255	T8756	T8757	arg1Of	Tat-positive,(
R7256	T8756	T8764	arg1Of	Tat-positive,and
R7257	T8759	T8760	arg1Of	siRNA,or
R7258	T8760	T8757	arg2Of	or,(
R7259	T8760	T8758	arg1Of	or,no
R7260	T8762	T8760	arg2Of	control,or
R7261	T8762	T8761	arg1Of	control,siRNA
R7262	T8763	T8757	arg3Of	),(
R7263	T8764	T8754	arg2Of	and,in
R7264	T8768	T8766	arg2Of	Tat,(
R7265	T8768	T8767	arg1Of	Tat,siRNA
R7266	T8769	T8766	arg3Of	),(
R7267	T8770	T8764	arg2Of	cells,and
R7268	T8770	T8765	arg1Of	cells,Tat-negative
R7269	T8770	T8766	arg1Of	cells,(
R7270	T8771	T8772	arg1Of	;,however
R7271	T8771	T8773	arg1Of	;,","
R7272	T8776	T8774	arg2Of	post-infection,at
R7273	T8776	T8775	arg1Of	post-infection,12-h
R7274	T8781	T8779	arg1Of	binding,p65
R7275	T8781	T8780	arg1Of	binding,DNA
R7276	T8781	T8782	arg1Of	binding,and
R7277	T8782	T8778	arg1Of	and,the
R7278	T8782	T8785	arg1Of	and,persisted
R7279	T8782	T8786	arg2Of	and,elevated
R7280	T8784	T8782	arg2Of	p65,and
R7281	T8784	T8783	arg1Of	p65,nuclear
R7282	T8785	T8771	arg2Of	persisted,;
R7283	T8785	T8774	arg1Of	persisted,at
R7284	T8785	T8777	arg1Of	persisted,","
R7285	T8785	T8786	modOf	persisted,elevated
R7286	T8785	T8798	arg1Of	persisted,","
R7287	T8785	T8799	arg1Of	persisted,while
R7288	T8786	T8787	arg1Of	elevated,in
R7289	T8787	T8789	arg1Of	in,of
R7290	T8788	T8787	arg2Of	presence,in
R7291	T8790	T8787	arg3Of	Tat,in
R7292	T8790	T8791	arg1Of	Tat,(
R7293	T8793	T8794	arg1Of	siRNA,and
R7294	T8794	T8791	arg2Of	and,(
R7295	T8794	T8792	arg1Of	and,no
R7296	T8796	T8794	arg2Of	control,and
R7297	T8796	T8795	arg1Of	control,siRNA
R7298	T8797	T8791	arg3Of	),(
R7299	T8800	T8801	arg1Of	they,dropped
R7300	T8801	T8799	arg2Of	dropped,while
R7301	T8801	T8802	arg1Of	dropped,in
R7302	T8802	T8804	arg1Of	in,of
R7303	T8803	T8802	arg2Of	absence,in
R7304	T8805	T8802	arg3Of	Tat,in
R7305	T8805	T8806	arg1Of	Tat,(
R7306	T8805	T8810	arg1Of	Tat,(
R7307	T8808	T8806	arg2Of	Tat,(
R7308	T8808	T8807	arg1Of	Tat,siRNA
R7309	T8809	T8806	arg3Of	),(
R7310	T8812	T8811	arg1Of	1B,Figure
R7311	T8812	T8813	arg1Of	1B,and
R7312	T8813	T8810	arg2Of	and,(
R7313	T8814	T8813	arg2Of	C,and
R7314	T8815	T8810	arg3Of	),(
R7315	T8816	T8817	arg1Of	IκB-α,was
R7316	T8816	T8818	arg2Of	IκB-α,degraded
R7317	T8816	T8828	arg2Of	IκB-α,synthesized
R7318	T8818	T8817	arg2Of	degraded,was
R7319	T8818	T8819	arg1Of	degraded,in
R7320	T8818	T8827	arg1Of	degraded,novo
R7321	T8818	T8828	modOf	degraded,synthesized
R7322	T8821	T8819	arg2Of	cytosol,in
R7323	T8821	T8820	arg1Of	cytosol,the
R7324	T8821	T8822	arg1Of	cytosol,within
R7325	T8824	T8822	arg2Of	post-infection,within
R7326	T8824	T8823	arg1Of	post-infection,3-h
R7327	T8827	T8825	arg1Of	novo,and
R7328	T8827	T8826	arg1Of	novo,de
R7329	T8828	T8829	arg1Of	synthesized,at
R7330	T8828	T8834	arg1Of	synthesized,at
R7331	T8830	T8829	arg2Of	6 h,at
R7332	T8830	T8831	arg1Of	6 h,with
R7333	T8833	T8831	arg2Of	replenishment,with
R7334	T8833	T8832	arg1Of	replenishment,full
R7335	T8836	T8834	arg2Of	post-infection,at
R7336	T8836	T8835	arg1Of	post-infection,12-h
R7337	T8836	T8837	arg1Of	post-infection,in
R7338	T8839	T8840	arg1Of	Tat-positive,and
R7339	T8841	T8840	arg2Of	Tat-negative,and
R7340	T8842	T8837	arg2Of	cells,in
R7341	T8842	T8838	arg1Of	cells,both
R7342	T8842	T8839	arg1Of	cells,Tat-positive
R7343	T8842	T8841	arg1Of	cells,Tat-negative
R7344	T8842	T8843	arg1Of	cells,(
R7345	T8845	T8843	arg2Of	1C,(
R7346	T8845	T8844	arg1Of	1C,Figure
R7347	T8845	T8846	arg1Of	1C,","
R7348	T8847	T8846	arg2Of	cytosol,","
R7349	T8848	T8843	arg3Of	),(
R7350	T8853	T8851	arg1Of	activity,the
R7351	T8853	T8852	arg1Of	activity,IKK
R7352	T8853	T8854	arg1Of	activity,was
R7353	T8853	T8855	arg2Of	activity,induced
R7354	T8853	T8861	arg1Of	activity,decreased
R7355	T8855	T8854	arg2Of	induced,was
R7356	T8855	T8856	arg1Of	induced,at
R7357	T8855	T8860	arg1Of	induced,and
R7358	T8858	T8856	arg2Of	post-infection,at
R7359	T8858	T8857	arg1Of	post-infection,1-h
R7360	T8860	T8849	arg1Of	and,Consistently
R7361	T8860	T8850	arg1Of	and,","
R7362	T8860	T8859	arg1Of	and,","
R7363	T8861	T8860	arg2Of	decreased,and
R7364	T8861	T8862	arg1Of	decreased,at
R7365	T8862	T8864	arg1Of	at,post-infection
R7366	T8863	T8862	arg2Of	3-h,at
R7367	T8864	T8865	arg1Of	post-infection,independently
R7368	T8865	T8866	arg1Of	independently,of
R7369	T8868	T8866	arg2Of	presence,of
R7370	T8868	T8867	arg1Of	presence,the
R7371	T8868	T8869	arg1Of	presence,of
R7372	T8870	T8869	arg2Of	Tat,of
R7373	T8870	T8871	arg1Of	Tat,(
R7374	T8873	T8871	arg2Of	1D,(
R7375	T8873	T8872	arg1Of	1D,Figure
R7376	T8874	T8871	arg3Of	),(
R7377	T8876	T8875	arg1Of	kinetics,Similar
R7378	T8876	T8877	arg1Of	kinetics,of
R7379	T8876	T8886	arg1Of	kinetics,were
R7380	T8876	T8887	arg2Of	kinetics,observed
R7381	T8879	T8878	arg1Of	activity,NF-κB
R7382	T8879	T8880	arg1Of	activity,","
R7383	T8880	T8883	arg1Of	",",and
R7384	T8882	T8880	arg2Of	activation,","
R7385	T8882	T8881	arg1Of	activation,IKK
R7386	T8883	T8877	arg2Of	and,of
R7387	T8885	T8883	arg2Of	degradation/re-synthesis,and
R7388	T8885	T8884	arg1Of	degradation/re-synthesis,IκB-α
R7389	T8887	T8886	arg2Of	observed,were
R7390	T8887	T8888	arg1Of	observed,in
R7391	T8891	T8888	arg2Of	infection,in
R7392	T8891	T8889	arg1Of	infection,single-round
R7393	T8891	T8890	arg1Of	infection,HIV-1
R7394	T8891	T8892	arg1Of	infection,of
R7395	T8893	T8892	arg2Of	PBMCs,of
R7396	T8893	T8894	arg1Of	PBMCs,using
R7397	T8894	T8907	arg1Of	using,as
R7398	T8896	T8894	arg2Of	cocktail,using
R7399	T8896	T8895	arg1Of	cocktail,a
R7400	T8896	T8897	arg1Of	cocktail,of
R7401	T8898	T8899	arg1Of	Azidothymidine,(
R7402	T8898	T8902	arg1Of	Azidothymidine,and
R7403	T8900	T8899	arg2Of	AZT,(
R7404	T8901	T8899	arg3Of	),(
R7405	T8902	T8897	arg2Of	and,of
R7406	T8903	T8902	arg2Of	Lamivudine,and
R7407	T8903	T8904	arg1Of	Lamivudine,(
R7408	T8905	T8904	arg2Of	3TC,(
R7409	T8906	T8904	arg3Of	),(
R7410	T8910	T8907	arg2Of	inhibitors,as
R7411	T8910	T8908	arg1Of	inhibitors,reverse
R7412	T8910	T8909	arg1Of	inhibitors,transcriptase
R7413	T8910	T8911	arg1Of	inhibitors,(
R7414	T8914	T8911	arg2Of	S2,(
R7415	T8914	T8912	arg1Of	S2,Supplementary
R7416	T8914	T8913	arg1Of	S2,Figure
R7417	T8915	T8911	arg3Of	),(
R7418	T8917	T8916	arg1Of	results,These
R7419	T8917	T8918	arg1Of	results,indicated
R7420	T8923	T8920	arg2Of	infection,in
R7421	T8923	T8921	arg1Of	infection,single-round
R7422	T8923	T8922	arg1Of	infection,HIV-1
R7423	T8926	T8924	arg1Of	activation,the
R7424	T8926	T8925	arg1Of	activation,NF-κB
R7425	T8926	T8928	arg1Of	activation,correlated
R7426	T8928	T8927	arg1Of	correlated,initially
R7427	T8928	T8929	arg1Of	correlated,with
R7428	T8928	T8936	arg1Of	correlated,independently
R7429	T8928	T8943	arg1Of	correlated,and
R7430	T8932	T8931	arg1Of	activation,IKK
R7431	T8932	T8933	arg1Of	activation,and
R7432	T8933	T8929	arg2Of	and,with
R7433	T8933	T8930	arg1Of	and,the
R7434	T8935	T8933	arg2Of	degradation,and
R7435	T8935	T8934	arg1Of	degradation,IκB-α
R7436	T8936	T8937	arg1Of	independently,of
R7437	T8939	T8937	arg2Of	presence,of
R7438	T8939	T8938	arg1Of	presence,the
R7439	T8939	T8940	arg1Of	presence,of
R7440	T8941	T8940	arg2Of	Tat,of
R7441	T8943	T8918	arg2Of	and,indicated
R7442	T8943	T8919	arg1Of	and,that
R7443	T8943	T8920	arg1Of	and,in
R7444	T8943	T8942	arg1Of	and,","
R7445	T8944	T8945	arg1Of	it,was
R7446	T8944	T8946	arg2Of	it,kept
R7447	T8944	T8947	arg2Of	it,elevated
R7448	T8946	T8943	arg2Of	kept,and
R7449	T8946	T8945	arg2Of	kept,was
R7450	T8947	T8946	arg3Of	elevated,kept
R7451	T8947	T8948	arg1Of	elevated,in
R7452	T8949	T8950	arg1Of	presence,of
R7453	T8949	T8952	arg1Of	presence,following
R7454	T8949	T8959	arg1Of	presence,and
R7455	T8951	T8950	arg2Of	Tat,of
R7456	T8954	T8952	arg2Of	decay,following
R7457	T8954	T8953	arg1Of	decay,the
R7458	T8954	T8955	arg1Of	decay,of
R7459	T8958	T8955	arg2Of	activity,of
R7460	T8958	T8956	arg1Of	activity,the
R7461	T8958	T8957	arg1Of	activity,IKK
R7462	T8959	T8948	arg2Of	and,in
R7463	T8961	T8959	arg2Of	synthesis,and
R7464	T8961	T8960	arg1Of	synthesis,new
R7465	T8961	T8962	arg1Of	synthesis,of
R7466	T8963	T8962	arg2Of	IκB-α,of
R7467	T8966	T8967	arg1Of	we,tested
R7468	T8967	T8964	arg1Of	tested,Next
R7469	T8967	T8965	arg1Of	tested,","
R7470	T8970	T8967	arg2Of	action,tested
R7471	T8970	T8968	arg1Of	action,the
R7472	T8970	T8969	arg1Of	action,single
R7473	T8970	T8971	arg1Of	action,of
R7474	T8970	T8973	arg1Of	action,on
R7475	T8972	T8971	arg2Of	Tat,of
R7476	T8975	T8973	arg2Of	kinetic,on
R7477	T8975	T8974	arg1Of	kinetic,the
R7478	T8975	T8976	arg1Of	kinetic,of
R7479	T8978	T8976	arg2Of	activity,of
R7480	T8978	T8977	arg1Of	activity,NF-κB
R7481	T8978	T8979	arg2Of	activity,induced
R7482	T8981	T8979	arg1Of	PMA,induced
R7483	T8981	T8980	arg2Of	PMA,by
R7484	T8984	T8982	arg2Of	end,To
R7485	T8984	T8983	arg1Of	end,this
R7486	T8987	T8986	arg1Of	cells,HeLa
R7487	T8987	T8988	arg1Of	cells,were
R7488	T8987	T8989	arg2Of	cells,transfected
R7489	T8989	T8982	arg1Of	transfected,To
R7490	T8989	T8985	arg1Of	transfected,","
R7491	T8989	T8988	arg2Of	transfected,were
R7492	T8989	T8990	arg1Of	transfected,with
R7493	T8991	T8993	arg1Of	p3x-FLAG-Tat,or
R7494	T8993	T8990	arg2Of	or,with
R7495	T8993	T8992	arg1Of	or,","
R7496	T8993	T8996	arg1Of	or,","
R7497	T8993	T8997	arg2Of	or,stimulated
R7498	T8993	T9005	arg2Of	or,washed
R7499	T8993	T9007	arg1Of	or,avoid
R7500	T8995	T8993	arg2Of	vector,or
R7501	T8995	T8994	arg1Of	vector,empty
R7502	T8997	T8998	arg1Of	stimulated,with
R7503	T8997	T9003	arg1Of	stimulated,and
R7504	T8999	T8998	arg2Of	PMA,with
R7505	T8999	T9000	arg1Of	PMA,for
R7506	T9001	T9000	arg2Of	5 min,for
R7507	T9003	T9002	arg1Of	and,","
R7508	T9005	T9003	arg2Of	washed,and
R7509	T9005	T9004	arg1Of	washed,extensively
R7510	T9007	T9005	arg3Of	avoid,washed
R7511	T9007	T9006	arg1Of	avoid,to
R7512	T9010	T9007	arg2Of	kinetic,avoid
R7513	T9010	T9008	arg1Of	kinetic,the
R7514	T9010	T9009	arg1Of	kinetic,oscillatory
R7515	T9010	T9011	arg1Of	kinetic,of
R7516	T9013	T9011	arg2Of	activation,of
R7517	T9013	T9012	arg1Of	activation,NF-κB
R7518	T9013	T9014	arg1Of	activation,(
R7519	T9015	T9014	arg2Of	"17,18",(
R7520	T9016	T9014	arg3Of	),(
R7521	T9019	T9017	arg2Of	cells,In
R7522	T9019	T9018	arg1Of	cells,un-stimulated
R7523	T9024	T9021	arg1Of	binding,the
R7524	T9024	T9022	arg1Of	binding,p65
R7525	T9024	T9023	arg1Of	binding,DNA
R7526	T9024	T9025	arg1Of	binding,(
R7527	T9024	T9029	arg1Of	binding,and
R7528	T9027	T9025	arg2Of	2A,(
R7529	T9027	T9026	arg1Of	2A,Figure
R7530	T9028	T9025	arg3Of	),(
R7531	T9029	T9038	arg1Of	and,were
R7532	T9029	T9040	arg2Of	and,enhanced
R7533	T9031	T9029	arg2Of	p65,and
R7534	T9031	T9030	arg1Of	p65,nuclear
R7535	T9031	T9032	arg1Of	p65,(
R7536	T9034	T9032	arg2Of	2B,(
R7537	T9034	T9033	arg1Of	2B,Figure
R7538	T9034	T9035	arg1Of	2B,","
R7539	T9036	T9035	arg2Of	nucleus,","
R7540	T9037	T9032	arg3Of	),(
R7541	T9040	T9017	arg1Of	enhanced,In
R7542	T9040	T9020	arg1Of	enhanced,","
R7543	T9040	T9038	arg2Of	enhanced,were
R7544	T9040	T9039	arg1Of	enhanced,slightly
R7545	T9040	T9043	arg1Of	enhanced,","
R7546	T9040	T9044	arg1Of	enhanced,while
R7547	T9040	T9052	arg1Of	enhanced,(
R7548	T9042	T9040	arg1Of	Tat,enhanced
R7549	T9042	T9041	arg2Of	Tat,by
R7550	T9047	T9045	arg1Of	content,the
R7551	T9047	T9046	arg1Of	content,IκB-α
R7552	T9047	T9048	arg1Of	content,was
R7553	T9047	T9051	arg2Of	content,affected
R7554	T9051	T9044	arg2Of	affected,while
R7555	T9051	T9048	arg2Of	affected,was
R7556	T9051	T9049	arg1Of	affected,not
R7557	T9051	T9050	arg1Of	affected,significantly
R7558	T9054	T9052	arg2Of	2B,(
R7559	T9054	T9053	arg1Of	2B,Figure
R7560	T9054	T9055	arg1Of	2B,","
R7561	T9056	T9055	arg2Of	cytosol,","
R7562	T9057	T9052	arg3Of	),(
R7563	T9059	T9058	arg1Of	stimulation,Transient
R7564	T9059	T9060	arg1Of	stimulation,with
R7565	T9059	T9062	arg1Of	stimulation,increased
R7566	T9061	T9060	arg2Of	PMA,with
R7567	T9062	T9076	arg1Of	increased,independently
R7568	T9062	T9088	arg1Of	increased,;
R7569	T9067	T9064	arg1Of	activity,NF-κB
R7570	T9067	T9065	arg1Of	activity,DNA
R7571	T9067	T9066	arg1Of	activity,binding
R7572	T9067	T9068	arg1Of	activity,and
R7573	T9068	T9062	arg2Of	and,increased
R7574	T9068	T9063	arg1Of	and,the
R7575	T9068	T9071	arg1Of	and,at
R7576	T9070	T9068	arg2Of	p65,and
R7577	T9070	T9069	arg1Of	p65,nuclear
R7578	T9072	T9071	arg2Of	5 min,at
R7579	T9072	T9073	arg1Of	5 min,peaking
R7580	T9073	T9074	arg1Of	peaking,at
R7581	T9075	T9074	arg2Of	60–120 min,at
R7582	T9076	T9077	arg1Of	independently,of
R7583	T9079	T9077	arg2Of	presence,of
R7584	T9079	T9078	arg1Of	presence,the
R7585	T9079	T9080	arg1Of	presence,of
R7586	T9081	T9080	arg2Of	Tat,of
R7587	T9081	T9082	arg1Of	Tat,(
R7588	T9084	T9083	arg1Of	2A,Figure
R7589	T9084	T9085	arg1Of	2A,and
R7590	T9085	T9082	arg2Of	and,(
R7591	T9086	T9085	arg2Of	B,and
R7592	T9087	T9082	arg3Of	),(
R7593	T9095	T9093	arg1Of	binding,p65
R7594	T9095	T9094	arg1Of	binding,DNA
R7595	T9095	T9096	arg1Of	binding,and
R7596	T9096	T9092	arg1Of	and,the
R7597	T9096	T9099	arg1Of	and,persisted
R7598	T9096	T9100	arg2Of	and,elevated
R7599	T9098	T9096	arg2Of	p65,and
R7600	T9098	T9097	arg1Of	p65,nuclear
R7601	T9099	T9091	arg2Of	persisted,while
R7602	T9099	T9100	modOf	persisted,elevated
R7603	T9099	T9101	arg1Of	persisted,at
R7604	T9099	T9104	arg1Of	persisted,in
R7605	T9103	T9101	arg2Of	post-treatment,at
R7606	T9103	T9102	arg1Of	post-treatment,240-min
R7607	T9106	T9104	arg2Of	cells,in
R7608	T9106	T9105	arg1Of	cells,Tat-positive
R7609	T9108	T9109	arg1Of	they,dropped
R7610	T9109	T9088	arg2Of	dropped,;
R7611	T9109	T9089	arg1Of	dropped,however
R7612	T9109	T9090	arg1Of	dropped,","
R7613	T9109	T9091	arg1Of	dropped,while
R7614	T9109	T9107	arg1Of	dropped,","
R7615	T9109	T9110	arg1Of	dropped,in
R7616	T9112	T9110	arg2Of	cells,in
R7617	T9112	T9111	arg1Of	cells,Tat-negative
R7618	T9112	T9113	arg1Of	cells,(
R7619	T9115	T9114	arg1Of	2A,Figure
R7620	T9115	T9116	arg1Of	2A,and
R7621	T9116	T9113	arg2Of	and,(
R7622	T9117	T9116	arg2Of	B,and
R7623	T9118	T9113	arg3Of	),(
R7624	T9120	T9119	arg2Of	addition,In
R7625	T9122	T9123	arg1Of	Tat,bound
R7626	T9123	T9124	arg1Of	bound,to
R7627	T9123	T9131	arg1Of	bound,and
R7628	T9127	T9124	arg2Of	oligonucleotide,to
R7629	T9127	T9125	arg1Of	oligonucleotide,the
R7630	T9127	T9126	arg1Of	oligonucleotide,NF-κB
R7631	T9127	T9128	arg1Of	oligonucleotide,in
R7632	T9130	T9128	arg2Of	cells,in
R7633	T9130	T9129	arg1Of	cells,un-stimulated
R7634	T9131	T9119	arg1Of	and,In
R7635	T9131	T9121	arg1Of	and,","
R7636	T9133	T9132	arg1Of	binding,its
R7637	T9133	T9134	arg1Of	binding,increased
R7638	T9134	T9131	arg2Of	increased,and
R7639	T9134	T9135	arg1Of	increased,following
R7640	T9134	T9142	arg1Of	increased,","
R7641	T9137	T9135	arg2Of	stimulation,following
R7642	T9137	T9136	arg1Of	stimulation,PMA
R7643	T9137	T9138	arg1Of	stimulation,(
R7644	T9140	T9138	arg2Of	2A,(
R7645	T9140	T9139	arg1Of	2A,Figure
R7646	T9141	T9138	arg3Of	),(
R7647	T9143	T9134	arg2Of	suggesting,increased
R7648	T9143	T9156	arg1Of	suggesting,to
R7649	T9147	T9145	arg1Of	protein,the
R7650	T9147	T9146	arg1Of	protein,viral
R7651	T9147	T9148	arg1Of	protein,was
R7652	T9148	T9143	arg2Of	was,suggesting
R7653	T9148	T9144	arg1Of	was,that
R7654	T9150	T9148	arg2Of	component,was
R7655	T9150	T9149	arg1Of	component,a
R7656	T9150	T9151	arg1Of	component,of
R7657	T9154	T9151	arg2Of	complex,of
R7658	T9154	T9152	arg1Of	complex,the
R7659	T9154	T9153	arg1Of	complex,NF-κB
R7660	T9154	T9155	arg2Of	complex,bound
R7661	T9157	T9156	arg2Of	DNA,to
R7662	T9158	T9159	arg1Of	Degradation,of
R7663	T9158	T9161	arg1Of	Degradation,was
R7664	T9158	T9163	arg2Of	Degradation,induced
R7665	T9160	T9159	arg2Of	IκB-α,of
R7666	T9163	T9161	arg2Of	induced,was
R7667	T9163	T9162	arg1Of	induced,progressively
R7668	T9163	T9164	arg1Of	induced,at
R7669	T9163	T9166	arg1Of	induced,","
R7670	T9163	T9167	modOf	induced,followed
R7671	T9165	T9164	arg2Of	5–30 min,at
R7672	T9170	T9169	arg1Of	novo,de
R7673	T9171	T9167	arg1Of	synthesis,followed
R7674	T9171	T9168	arg2Of	synthesis,by
R7675	T9171	T9170	arg1Of	synthesis,novo
R7676	T9171	T9172	arg1Of	synthesis,at
R7677	T9173	T9172	arg2Of	60 min,at
R7678	T9173	T9174	arg1Of	60 min,with
R7679	T9176	T9174	arg2Of	replenishment,with
R7680	T9176	T9175	arg1Of	replenishment,full
R7681	T9176	T9177	arg1Of	replenishment,at
R7682	T9178	T9177	arg2Of	240 min,at
R7683	T9178	T9179	arg1Of	240 min,in
R7684	T9181	T9182	arg1Of	Tat-positive,and
R7685	T9183	T9182	arg2Of	negative,and
R7686	T9184	T9179	arg2Of	cells,in
R7687	T9184	T9180	arg1Of	cells,both
R7688	T9184	T9181	arg1Of	cells,Tat-positive
R7689	T9184	T9183	arg1Of	cells,negative
R7690	T9184	T9185	arg1Of	cells,(
R7691	T9187	T9185	arg2Of	2B,(
R7692	T9187	T9186	arg1Of	2B,Figure
R7693	T9187	T9188	arg1Of	2B,","
R7694	T9189	T9188	arg2Of	cytosol,","
R7695	T9190	T9185	arg3Of	),(
R7696	T9195	T9193	arg1Of	activation,the
R7697	T9195	T9194	arg1Of	activation,IKK
R7698	T9195	T9196	arg1Of	activation,started
R7699	T9195	T9201	arg1Of	activation,turned
R7700	T9196	T9197	arg1Of	started,at
R7701	T9196	T9200	arg1Of	started,and
R7702	T9198	T9197	arg2Of	5 min,at
R7703	T9200	T9191	arg1Of	and,Consistently
R7704	T9200	T9192	arg1Of	and,","
R7705	T9200	T9199	arg1Of	and,","
R7706	T9201	T9200	arg2Of	turned,and
R7707	T9201	T9202	arg1Of	turned,off
R7708	T9201	T9203	arg1Of	turned,at
R7709	T9201	T9205	arg1Of	turned,independently
R7710	T9204	T9203	arg2Of	30 min,at
R7711	T9205	T9206	arg1Of	independently,of
R7712	T9208	T9206	arg2Of	presence,of
R7713	T9208	T9207	arg1Of	presence,the
R7714	T9208	T9209	arg1Of	presence,of
R7715	T9210	T9209	arg2Of	Tat,of
R7716	T9210	T9211	arg1Of	Tat,(
R7717	T9213	T9211	arg2Of	2C,(
R7718	T9213	T9212	arg1Of	2C,Figure
R7719	T9214	T9211	arg3Of	),(
R7720	T9216	T9215	arg1Of	results,These
R7721	T9216	T9217	arg1Of	results,indicated
R7722	T9219	T9220	arg1Of	Tat,enhanced
R7723	T9219	T9230	arg1Of	Tat,affecting
R7724	T9220	T9217	arg2Of	enhanced,indicated
R7725	T9220	T9218	arg1Of	enhanced,that
R7726	T9220	T9224	arg1Of	enhanced,in
R7727	T9220	T9229	arg1Of	enhanced,without
R7728	T9223	T9220	arg2Of	activity,enhanced
R7729	T9223	T9221	arg1Of	activity,the
R7730	T9223	T9222	arg1Of	activity,NF-κB
R7731	T9225	T9226	arg1Of	un-stimulated,and
R7732	T9227	T9226	arg2Of	PMA-stimulated,and
R7733	T9228	T9224	arg2Of	cells,in
R7734	T9228	T9225	arg1Of	cells,un-stimulated
R7735	T9228	T9227	arg1Of	cells,PMA-stimulated
R7736	T9230	T9229	arg2Of	affecting,without
R7737	T9233	T9232	arg1Of	activity,IKK
R7738	T9233	T9234	arg1Of	activity,and
R7739	T9234	T9230	arg2Of	and,affecting
R7740	T9234	T9231	arg1Of	and,the
R7741	T9236	T9234	arg2Of	content,and
R7742	T9236	T9235	arg1Of	content,IκB-α
R7743	T9238	T9240	arg1Of	we,found
R7744	T9240	T9237	arg2Of	found,As
R7745	T9240	T9239	arg1Of	found,previously
R7746	T9242	T9243	arg1Of	Tat,binds
R7747	T9243	T9240	arg2Of	binds,found
R7748	T9243	T9241	arg1Of	binds,that
R7749	T9243	T9244	arg1Of	binds,to
R7750	T9247	T9244	arg2Of	ankyrin,to
R7751	T9247	T9245	arg1Of	ankyrin,the
R7752	T9247	T9246	arg1Of	ankyrin,sixth
R7753	T9247	T9248	arg1Of	ankyrin,of
R7754	T9249	T9248	arg2Of	IκB-α,of
R7755	T9249	T9250	arg1Of	IκB-α,(
R7756	T9251	T9250	arg2Of	"50,51",(
R7757	T9252	T9250	arg3Of	),(
R7758	T9254	T9255	arg1Of	we,tested
R7759	T9255	T9237	arg1Of	tested,As
R7760	T9255	T9253	arg1Of	tested,","
R7761	T9257	T9258	arg1Of	Tat,counteracted
R7762	T9258	T9255	arg2Of	counteracted,tested
R7763	T9258	T9256	arg1Of	counteracted,whether
R7764	T9260	T9258	arg2Of	generation,counteracted
R7765	T9260	T9259	arg1Of	generation,the
R7766	T9260	T9261	arg1Of	generation,of
R7767	T9264	T9261	arg2Of	complex,of
R7768	T9264	T9262	arg1Of	complex,the
R7769	T9264	T9263	arg1Of	complex,IκB-α/NF-κB
R7770	T9267	T9265	arg2Of	end,To
R7771	T9267	T9266	arg1Of	end,this
R7772	T9270	T9269	arg1Of	association,the
R7773	T9270	T9271	arg1Of	association,of
R7774	T9270	T9273	arg1Of	association,with
R7775	T9270	T9275	arg1Of	association,was
R7776	T9270	T9276	arg2Of	association,analysed
R7777	T9272	T9271	arg2Of	IκB-α,of
R7778	T9274	T9273	arg2Of	p65,with
R7779	T9276	T9265	arg1Of	analysed,To
R7780	T9276	T9268	arg1Of	analysed,","
R7781	T9276	T9275	arg2Of	analysed,was
R7782	T9276	T9278	arg1Of	analysed,vivo
R7783	T9276	T9279	arg1Of	analysed,at
R7784	T9276	T9283	arg1Of	analysed,after
R7785	T9278	T9277	arg1Of	vivo,in
R7786	T9280	T9281	arg1Of	0,and
R7787	T9281	T9279	arg2Of	and,at
R7788	T9282	T9281	arg2Of	240 min,and
R7789	T9285	T9283	arg2Of	PMA,after
R7790	T9285	T9284	arg1Of	PMA,short-pulse
R7791	T9285	T9286	arg1Of	PMA,","
R7792	T9285	T9287	arg1Of	PMA,which
R7793	T9285	T9288	arg1Of	PMA,corresponded
R7794	T9288	T9289	arg1Of	corresponded,to
R7795	T9288	T9302	arg1Of	corresponded,","
R7796	T9288	T9303	arg1Of	corresponded,respectively
R7797	T9291	T9289	arg2Of	time,to
R7798	T9291	T9290	arg1Of	time,the
R7799	T9291	T9292	arg1Of	time,of
R7800	T9294	T9293	arg1Of	condition,un-stimulated
R7801	T9294	T9295	arg1Of	condition,and
R7802	T9295	T9292	arg2Of	and,of
R7803	T9298	T9297	arg1Of	novo,de
R7804	T9299	T9295	arg2Of	synthesis,and
R7805	T9299	T9296	arg1Of	synthesis,post-activation
R7806	T9299	T9298	arg1Of	synthesis,novo
R7807	T9299	T9300	arg1Of	synthesis,of
R7808	T9301	T9300	arg2Of	IκB-α,of
R7809	T9304	T9305	arg1Of	Coimmunoprecipitation,of
R7810	T9304	T9307	arg1Of	Coimmunoprecipitation,with
R7811	T9304	T9309	arg1Of	Coimmunoprecipitation,was
R7812	T9304	T9310	arg2Of	Coimmunoprecipitation,detected
R7813	T9304	T9330	arg1Of	Coimmunoprecipitation,was
R7814	T9304	T9331	arg2Of	Coimmunoprecipitation,halved
R7815	T9306	T9305	arg2Of	IκB-α,of
R7816	T9308	T9307	arg2Of	p65,with
R7817	T9310	T9309	arg2Of	detected,was
R7818	T9310	T9311	arg1Of	detected,at
R7819	T9310	T9329	arg1Of	detected,and
R7820	T9312	T9313	arg1Of	0-,and
R7821	T9313	T9311	arg2Of	and,at
R7822	T9314	T9313	arg2Of	240-min,and
R7823	T9314	T9315	arg1Of	240-min,post-treatment
R7824	T9315	T9316	arg1Of	post-treatment,in
R7825	T9318	T9316	arg2Of	cells,in
R7826	T9318	T9317	arg1Of	cells,Tat-negative
R7827	T9318	T9319	arg1Of	cells,(
R7828	T9321	T9319	arg2Of	2D,(
R7829	T9321	T9320	arg1Of	2D,Figure
R7830	T9321	T9322	arg1Of	2D,","
R7831	T9324	T9325	arg1Of	1,and
R7832	T9325	T9322	arg2Of	and,","
R7833	T9325	T9323	arg1Of	and,lanes
R7834	T9326	T9325	arg2Of	3,and
R7835	T9327	T9319	arg3Of	),(
R7836	T9329	T9328	arg1Of	and,","
R7837	T9329	T9350	arg1Of	and,","
R7838	T9329	T9351	modOf	and,indicating
R7839	T9331	T9329	arg2Of	halved,and
R7840	T9331	T9330	arg2Of	halved,was
R7841	T9331	T9332	arg1Of	halved,in
R7842	T9334	T9332	arg2Of	cells,in
R7843	T9334	T9333	arg1Of	cells,Tat-positive
R7844	T9334	T9335	arg1Of	cells,","
R7845	T9334	T9336	arg1Of	cells,where
R7846	T9334	T9341	arg1Of	cells,(
R7847	T9337	T9338	arg1Of	Tat,coimmunoprecipitated
R7848	T9338	T9336	arg2Of	coimmunoprecipitated,where
R7849	T9338	T9339	arg1Of	coimmunoprecipitated,with
R7850	T9340	T9339	arg2Of	IκB-α,with
R7851	T9343	T9341	arg2Of	2D,(
R7852	T9343	T9342	arg1Of	2D,Figure
R7853	T9343	T9344	arg1Of	2D,","
R7854	T9346	T9347	arg1Of	2,and
R7855	T9347	T9344	arg2Of	and,","
R7856	T9347	T9345	arg1Of	and,lanes
R7857	T9348	T9347	arg2Of	4,and
R7858	T9349	T9341	arg3Of	),(
R7859	T9353	T9354	arg1Of	Tat,competed
R7860	T9354	T9351	arg2Of	competed,indicating
R7861	T9354	T9352	arg1Of	competed,that
R7862	T9357	T9354	arg2Of	binding,competed
R7863	T9357	T9355	arg1Of	binding,the
R7864	T9357	T9356	arg1Of	binding,IκB-α
R7865	T9357	T9358	arg1Of	binding,to
R7866	T9359	T9358	arg2Of	p65,to
R9460	T11613	T11614	arg1Of	Tat,counteracts
R9461	T11613	T11621	arg1Of	Tat,competing
R9462	T11614	T11620	arg1Of	counteracts,by
R9463	T11617	T11614	arg2Of	inhibition,counteracts
R9464	T11617	T11615	arg1Of	inhibition,the
R9465	T11617	T11616	arg1Of	inhibition,IκB-α
R9466	T11617	T11618	arg1Of	inhibition,of
R9467	T11619	T11618	arg2Of	p65,of
R9468	T11621	T11620	arg2Of	competing,by
R9469	T11624	T11621	arg2Of	binding,competing
R9470	T11624	T11622	arg1Of	binding,the
R9471	T11624	T11623	arg1Of	binding,repressor
R9472	T11625	T11627	arg1Of	We,investigated
R9473	T11627	T11626	arg1Of	investigated,further
R9474	T11630	T11627	arg2Of	interaction,investigated
R9475	T11630	T11628	arg1Of	interaction,the
R9476	T11630	T11629	arg1Of	interaction,physical
R9477	T11630	T11631	arg1Of	interaction,of
R9478	T11630	T11633	arg1Of	interaction,with
R9479	T11632	T11631	arg2Of	Tat,of
R9480	T11634	T11633	arg2Of	IκB-α,with
R9481	T11634	T11635	arg1Of	IκB-α,using
R9482	T11635	T11640	arg1Of	using,in
R9483	T11637	T11636	arg1Of	vitro,in
R9484	T11639	T11635	arg2Of	proteins,using
R9485	T11639	T11637	arg1Of	proteins,vitro
R9486	T11639	T11638	arg2Of	proteins,translated
R9487	T11642	T11640	arg2Of	assays,in
R9488	T11642	T11641	arg1Of	assays,coimmunoprecipitation
R9489	T11644	T11643	arg2Of	mapping,For
R9490	T11647	T11644	arg2Of	domains,mapping
R9491	T11647	T11645	arg1Of	domains,the
R9492	T11647	T11646	arg1Of	domains,interaction
R9493	T11649	T11650	arg1Of	we,used
R9494	T11650	T11643	arg1Of	used,For
R9495	T11650	T11648	arg1Of	used,","
R9496	T11650	T11655	arg1Of	used,:
R9497	T11654	T11650	arg2Of	mutants,used
R9498	T11654	T11651	arg1Of	mutants,the
R9499	T11654	T11652	arg1Of	mutants,following
R9500	T11654	T11653	arg1Of	mutants,Tat
R9501	T11657	T11656	arg1Of	T,Tat
R9502	T11657	T11658	arg1Of	T,","
R9503	T11657	T11664	arg1Of	T,","
R9504	T11661	T11660	arg2Of	"23,24",(
R9505	T11662	T11660	arg3Of	),(
R9506	T11663	T11658	arg2Of	A,","
R9507	T11663	T11659	arg1Of	A,N
R9508	T11663	T11660	arg1Of	A,(
R9509	T11664	T11671	arg1Of	",",","
R9510	T11668	T11667	arg2Of	"22,25,27",(
R9511	T11669	T11667	arg3Of	),(
R9512	T11670	T11664	arg2Of	A,","
R9513	T11670	T11665	arg1Of	A,Tat
R9514	T11670	T11666	arg1Of	A,C
R9515	T11670	T11667	arg1Of	A,(
R9516	T11675	T11674	arg2Of	"50,51",(
R9517	T11676	T11674	arg3Of	),(
R9518	T11677	T11672	arg1Of	A,Tat
R9519	T11677	T11673	arg1Of	A,K
R9520	T11677	T11674	arg1Of	A,(
R9521	T11677	T11678	arg1Of	A,and
R9522	T11678	T11671	arg2Of	and,","
R9523	T11680	T11679	arg1Of	R,Tat
R9524	T11680	T11681	arg1Of	R,(
R9525	T11682	T11681	arg2Of	49–57,(
R9526	T11683	T11681	arg3Of	),(
R9527	T11684	T11678	arg2Of	A,and
R9528	T11684	T11680	arg1Of	A,R
R9529	T11684	T11685	arg1Of	A,(
R9530	T11687	T11685	arg2Of	3A,(
R9531	T11687	T11686	arg1Of	3A,Figure
R9532	T11688	T11685	arg3Of	),(
R9533	T11689	T11690	arg1Of	IκB-α,coimmunoprecipitated
R9534	T11690	T11691	arg1Of	coimmunoprecipitated,with
R9535	T11690	T11695	arg1Of	coimmunoprecipitated,","
R9536	T11694	T11691	arg2Of	proteins,with
R9537	T11694	T11692	arg1Of	proteins,all
R9538	T11694	T11693	arg1Of	proteins,Tat
R9539	T11698	T11697	arg1Of	R,Tat
R9540	T11698	T11699	arg1Of	R,(
R9541	T11700	T11699	arg2Of	49–57,(
R9542	T11701	T11699	arg3Of	),(
R9543	T11702	T11690	arg2Of	A,coimmunoprecipitated
R9544	T11702	T11696	arg1Of	A,except
R9545	T11702	T11698	arg1Of	A,R
R9546	T11702	T11703	arg1Of	A,(
R9547	T11705	T11703	arg2Of	3B,(
R9548	T11705	T11704	arg1Of	3B,Figure
R9549	T11706	T11703	arg3Of	),(
R9550	T11708	T11707	arg1Of	results,These
R9551	T11708	T11709	arg1Of	results,indicated
R9552	T11713	T11711	arg1Of	domain,the
R9553	T11713	T11712	arg1Of	domain,arginine-rich
R9554	T11713	T11714	arg1Of	domain,of
R9555	T11713	T11716	arg1Of	domain,was
R9556	T11713	T11717	arg2Of	domain,involved
R9557	T11715	T11714	arg2Of	Tat,of
R9558	T11717	T11709	arg2Of	involved,indicated
R9559	T11717	T11710	arg1Of	involved,that
R9560	T11717	T11716	arg2Of	involved,was
R9561	T11717	T11718	arg1Of	involved,in
R9562	T11720	T11718	arg2Of	binding,in
R9563	T11720	T11719	arg1Of	binding,the
R9564	T11720	T11721	arg1Of	binding,to
R9565	T11720	T11723	arg1Of	binding,","
R9566	T11720	T11724	arg1Of	binding,which
R9567	T11720	T11725	arg1Of	binding,was
R9568	T11720	T11726	arg1Of	binding,consistent
R9569	T11722	T11721	arg2Of	IκB-α,to
R9570	T11726	T11725	arg2Of	consistent,was
R9571	T11726	T11727	arg1Of	consistent,with
R9572	T11729	T11727	arg2Of	observations,with
R9573	T11729	T11728	arg1Of	observations,previous
R9574	T11729	T11730	arg1Of	observations,(
R9575	T11731	T11730	arg2Of	"50,51",(
R9576	T11732	T11730	arg3Of	),(
R9577	T11735	T11736	arg1Of	Tat,associating
R9578	T11735	T11739	arg1Of	Tat,competed
R9579	T11736	T11737	arg1Of	associating,with
R9580	T11738	T11737	arg2Of	IκB-α,with
R9581	T11739	T11733	arg1Of	competed,Moreover
R9582	T11739	T11734	arg1Of	competed,","
R9583	T11739	T11757	arg1Of	competed,","
R9584	T11739	T11758	arg1Of	competed,while
R9585	T11741	T11739	arg2Of	binding,competed
R9586	T11741	T11740	arg1Of	binding,the
R9587	T11741	T11742	arg1Of	binding,of
R9588	T11741	T11744	arg1Of	binding,to
R9589	T11741	T11746	arg1Of	binding,in
R9590	T11743	T11742	arg2Of	IκB-α,of
R9591	T11745	T11744	arg2Of	p65,to
R9592	T11749	T11746	arg2Of	manner,in
R9593	T11749	T11747	arg1Of	manner,a
R9594	T11749	T11748	arg1Of	manner,dose-dependent
R9595	T11749	T11750	arg1Of	manner,(
R9596	T11752	T11750	arg2Of	3C,(
R9597	T11752	T11751	arg1Of	3C,Figure
R9598	T11752	T11753	arg1Of	3C,","
R9599	T11754	T11753	arg2Of	lanes,","
R9600	T11754	T11755	arg1Of	lanes,3–5
R9601	T11756	T11750	arg3Of	),(
R9602	T11760	T11759	arg1Of	R,Tat
R9603	T11760	T11761	arg1Of	R,(
R9604	T11762	T11761	arg2Of	49–57,(
R9605	T11763	T11761	arg3Of	),(
R9606	T11764	T11760	arg1Of	A,R
R9607	T11764	T11765	arg1Of	A,","
R9608	T11764	T11766	arg1Of	A,which
R9609	T11764	T11767	arg1Of	A,lacked
R9610	T11764	T11774	arg1Of	A,did
R9611	T11764	T11776	arg1Of	A,associate
R9612	T11764	T11780	arg1Of	A,did
R9613	T11764	T11782	arg1Of	A,compete
R9614	T11770	T11767	arg2Of	site,lacked
R9615	T11770	T11768	arg1Of	site,the
R9616	T11770	T11769	arg1Of	site,binding
R9617	T11770	T11771	arg1Of	site,for
R9618	T11772	T11771	arg2Of	IκB-α,for
R9619	T11776	T11774	arg2Of	associate,did
R9620	T11776	T11775	arg1Of	associate,not
R9621	T11776	T11777	arg1Of	associate,with
R9622	T11776	T11779	arg1Of	associate,and
R9623	T11778	T11777	arg2Of	IκB-α,with
R9624	T11779	T11758	arg2Of	and,while
R9625	T11779	T11773	arg1Of	and,","
R9626	T11782	T11779	arg2Of	compete,and
R9627	T11782	T11780	arg2Of	compete,did
R9628	T11782	T11781	arg1Of	compete,not
R9629	T11784	T11782	arg2Of	binding,compete
R9630	T11784	T11783	arg1Of	binding,the
R9631	T11784	T11785	arg1Of	binding,of
R9632	T11784	T11787	arg1Of	binding,to
R9633	T11786	T11785	arg2Of	IκB-α,of
R9634	T11788	T11787	arg2Of	p65,to
R9635	T11788	T11789	arg1Of	p65,(
R9636	T11791	T11789	arg2Of	3C,(
R9637	T11791	T11790	arg1Of	3C,Figure
R9638	T11791	T11792	arg1Of	3C,","
R9639	T11793	T11792	arg2Of	lanes,","
R9640	T11793	T11794	arg1Of	lanes,6–8
R9641	T11795	T11789	arg3Of	),(
R9642	T11798	T11799	arg1Of	we,evaluated
R9643	T11799	T11796	arg1Of	evaluated,Next
R9644	T11799	T11797	arg1Of	evaluated,","
R9645	T11801	T11802	arg1Of	Tat,counteracted
R9646	T11801	T11812	arg1Of	Tat,incubating
R9647	T11802	T11799	arg2Of	counteracted,evaluated
R9648	T11802	T11800	arg1Of	counteracted,whether
R9649	T11802	T11811	arg1Of	counteracted,by
R9650	T11805	T11802	arg2Of	inhibition,counteracted
R9651	T11805	T11803	arg1Of	inhibition,the
R9652	T11805	T11804	arg1Of	inhibition,IκB-α-mediated
R9653	T11805	T11806	arg1Of	inhibition,of
R9654	T11805	T11809	arg1Of	inhibition,to
R9655	T11808	T11806	arg2Of	binding,of
R9656	T11808	T11807	arg1Of	binding,p65
R9657	T11810	T11809	arg2Of	DNA,to
R9658	T11812	T11811	arg2Of	incubating,by
R9659	T11812	T11824	arg1Of	incubating,in
R9660	T11814	T11813	arg1Of	vitro,in
R9661	T11816	T11815	arg2Of	p65,translated
R9662	T11816	T11817	arg1Of	p65,and
R9663	T11817	T11812	arg2Of	and,incubating
R9664	T11817	T11814	arg1Of	and,vitro
R9665	T11817	T11820	arg1Of	and,with
R9666	T11819	T11817	arg2Of	proteins,and
R9667	T11819	T11818	arg1Of	proteins,IκB-α
R9668	T11823	T11820	arg2Of	probe,with
R9669	T11823	T11821	arg1Of	probe,the
R9670	T11823	T11822	arg1Of	probe,NF-κB
R9671	T11825	T11826	arg1Of	presence,or
R9672	T11826	T11824	arg2Of	or,in
R9673	T11826	T11828	arg1Of	or,of
R9674	T11827	T11826	arg2Of	absence,or
R9675	T11829	T11828	arg2Of	Tat,of
R9676	T11829	T11830	arg2Of	Tat,followed
R9677	T11832	T11830	arg1Of	EMSA,followed
R9678	T11832	T11831	arg2Of	EMSA,by
R9679	T11837	T11833	arg1Of	activity,The
R9680	T11837	T11834	arg1Of	activity,p65
R9681	T11837	T11835	arg1Of	activity,DNA
R9682	T11837	T11836	arg1Of	activity,binding
R9683	T11837	T11838	arg1Of	activity,was
R9684	T11837	T11839	arg2Of	activity,inhibited
R9685	T11837	T11843	arg2Of	activity,restored
R9686	T11839	T11842	arg1Of	inhibited,and
R9687	T11841	T11839	arg1Of	IκB-α,inhibited
R9688	T11841	T11840	arg2Of	IκB-α,by
R9689	T11842	T11838	arg2Of	and,was
R9690	T11843	T11842	arg2Of	restored,and
R9691	T11843	T11844	arg1Of	restored,in
R9692	T11843	T11848	arg1Of	restored,by
R9693	T11843	T11854	arg1Of	restored,by
R9694	T11847	T11844	arg2Of	manner,in
R9695	T11847	T11845	arg1Of	manner,a
R9696	T11847	T11846	arg1Of	manner,dose-dependent
R9697	T11848	T11852	arg1Of	by,and
R9698	T11850	T11848	arg2Of	Tat,by
R9699	T11850	T11849	arg1Of	Tat,wild-type
R9700	T11852	T11851	arg1Of	and,","
R9701	T11854	T11852	arg2Of	by,and
R9702	T11854	T11853	arg1Of	by,not
R9703	T11856	T11854	arg2Of	R,by
R9704	T11856	T11855	arg1Of	R,Tat
R9705	T11856	T11857	arg1Of	R,(
R9706	T11856	T11860	arg1Of	R,(
R9707	T11858	T11857	arg2Of	49–57,(
R9708	T11859	T11857	arg3Of	),(
R9709	T11862	T11860	arg2Of	3D,(
R9710	T11862	T11861	arg1Of	3D,Figure
R9711	T11863	T11860	arg3Of	),(
R9712	T11866	T11867	arg1Of	we,analysed
R9713	T11866	T11880	arg1Of	we,transfecting
R9714	T11867	T11864	arg1Of	analysed,Further
R9715	T11867	T11865	arg1Of	analysed,","
R9716	T11867	T11871	arg1Of	analysed,on
R9717	T11867	T11879	arg1Of	analysed,by
R9718	T11870	T11867	arg2Of	effect,analysed
R9719	T11870	T11868	arg1Of	effect,the
R9720	T11870	T11869	arg1Of	effect,Tat
R9721	T11874	T11871	arg2Of	repression,on
R9722	T11874	T11872	arg1Of	repression,the
R9723	T11874	T11873	arg1Of	repression,IκB-α
R9724	T11874	T11875	arg1Of	repression,of
R9725	T11878	T11875	arg2Of	activity,of
R9726	T11878	T11876	arg1Of	activity,p65
R9727	T11878	T11877	arg1Of	activity,transcriptional
R9728	T11880	T11879	arg2Of	transfecting,by
R9729	T11880	T11882	arg1Of	transfecting,with
R9730	T11880	T11886	arg1Of	transfecting,together
R9731	T11880	T11887	arg1Of	transfecting,with
R9732	T11880	T11894	arg1Of	transfecting,","
R9733	T11880	T11895	arg1Of	transfecting,in
R9734	T11881	T11880	arg2Of	p50−/−p65−/−MEFs,transfecting
R9735	T11885	T11882	arg2Of	reporter,with
R9736	T11885	T11883	arg1Of	reporter,the
R9737	T11885	T11884	arg1Of	reporter,NF-κB-Luc
R9738	T11889	T11887	arg2Of	vectors,with
R9739	T11889	T11888	arg1Of	vectors,expression
R9740	T11889	T11890	arg1Of	vectors,of
R9741	T11891	T11892	arg1Of	p65,and
R9742	T11892	T11890	arg2Of	and,of
R9743	T11893	T11892	arg2Of	IκB-α,and
R9744	T11896	T11897	arg1Of	presence,or
R9745	T11897	T11899	arg1Of	or,of
R9746	T11897	T11902	arg1Of	or,inhibited
R9747	T11898	T11897	arg2Of	absence,or
R9748	T11901	T11899	arg2Of	IκB-α,of
R9749	T11901	T11900	arg1Of	IκB-α,Tat.
R9750	T11902	T11895	arg2Of	inhibited,in
R9751	T11905	T11902	arg2Of	expression,inhibited
R9752	T11905	T11903	arg1Of	expression,the
R9753	T11905	T11904	arg1Of	expression,p65-dependent
R9754	T11905	T11906	arg1Of	expression,of
R9755	T11905	T11924	arg1Of	expression,by
R9756	T11906	T11922	arg1Of	of,and
R9757	T11909	T11906	arg2Of	gene,of
R9758	T11909	T11907	arg1Of	gene,the
R9759	T11909	T11908	arg1Of	gene,luciferase
R9760	T11909	T11910	arg1Of	gene,","
R9761	T11909	T11911	arg1Of	gene,which
R9762	T11909	T11912	arg1Of	gene,was
R9763	T11909	T11913	arg2Of	gene,restored
R9764	T11913	T11912	arg2Of	restored,was
R9765	T11913	T11914	arg1Of	restored,in
R9766	T11917	T11914	arg2Of	manner,in
R9767	T11917	T11915	arg1Of	manner,a
R9768	T11917	T11916	arg1Of	manner,dose-dependent
R9769	T11920	T11913	arg1Of	Tat,restored
R9770	T11920	T11918	arg2Of	Tat,by
R9771	T11920	T11919	arg1Of	Tat,wild-type
R9772	T11922	T11921	arg1Of	and,","
R9773	T11924	T11922	arg2Of	by,and
R9774	T11924	T11923	arg1Of	by,not
R9775	T11926	T11925	arg1Of	R,Tat
R9776	T11926	T11927	arg1Of	R,(
R9777	T11928	T11927	arg2Of	49–57,(
R9778	T11929	T11927	arg3Of	),(
R9779	T11930	T11924	arg2Of	A,by
R9780	T11930	T11926	arg1Of	A,R
R9781	T11930	T11931	arg1Of	A,(
R9782	T11933	T11931	arg2Of	3E,(
R9783	T11933	T11932	arg1Of	3E,Figure
R9784	T11934	T11931	arg3Of	),(
R9785	T11937	T11936	arg1Of	results,these
R9786	T11937	T11938	arg1Of	results,indicated
R9787	T11938	T11935	arg1Of	indicated,Altogether
R9788	T11940	T11941	arg1Of	Tat,counteracts
R9789	T11940	T11953	arg1Of	Tat,associating
R9790	T11940	T11957	arg1Of	Tat,competing
R9791	T11941	T11938	arg2Of	counteracts,indicated
R9792	T11941	T11939	arg1Of	counteracts,that
R9793	T11941	T11952	arg1Of	counteracts,by
R9794	T11944	T11941	arg2Of	repression,counteracts
R9795	T11944	T11942	arg1Of	repression,the
R9796	T11944	T11943	arg1Of	repression,IκB-α
R9797	T11944	T11945	arg1Of	repression,of
R9798	T11948	T11949	arg1Of	DNA-binding,and
R9799	T11950	T11949	arg2Of	transcriptional,and
R9800	T11951	T11945	arg2Of	activity,of
R9801	T11951	T11946	arg1Of	activity,the
R9802	T11951	T11947	arg1Of	activity,p65
R9803	T11951	T11948	arg1Of	activity,DNA-binding
R9804	T11951	T11950	arg1Of	activity,transcriptional
R9805	T11953	T11954	arg1Of	associating,with
R9806	T11953	T11956	arg1Of	associating,and
R9807	T11955	T11954	arg2Of	IκB-α,with
R9808	T11956	T11952	arg2Of	and,by
R9809	T11957	T11956	arg2Of	competing,and
R9810	T11960	T11957	arg2Of	binding,competing
R9811	T11960	T11958	arg1Of	binding,the
R9812	T11960	T11959	arg1Of	binding,repressor
R9813	T11960	T11961	arg1Of	binding,to
R9814	T11962	T11961	arg2Of	p65,to
R10763	T13335	T13336	arg1Of	Tat,increases
R10764	T13336	T13343	arg1Of	increases,through
R10765	T13340	T13336	arg2Of	binding,increases
R10766	T13340	T13337	arg1Of	binding,the
R10767	T13340	T13338	arg1Of	binding,p65
R10768	T13340	T13339	arg1Of	binding,affinity
R10769	T13340	T13341	arg1Of	binding,to
R10770	T13342	T13341	arg2Of	DNA,to
R10772	T13344	T13343	arg2Of	association,through
R10773	T13346	T13345	arg2Of	p65,with
R10774	T13347	T13348	arg1Of	We,tested
R10775	T13350	T13352	arg1Of	Tat,interacted
R10776	T13352	T13348	arg2Of	interacted,tested
R10777	T13352	T13349	arg1Of	interacted,whether
R10778	T13352	T13351	arg1Of	interacted,physically
R10779	T13352	T13353	arg1Of	interacted,with
R10780	T13354	T13353	arg2Of	p65,with
R10781	T13356	T13355	arg1Of	cells,HeLa
R10782	T13356	T13357	arg1Of	cells,were
R10783	T13356	T13358	arg2Of	cells,transfected
R10784	T13358	T13357	arg2Of	transfected,were
R10785	T13358	T13359	arg1Of	transfected,with
R10786	T13358	T13368	arg1Of	transfected,and
R10787	T13362	T13359	arg2Of	vectors,with
R10788	T13362	T13360	arg1Of	vectors,the
R10789	T13362	T13361	arg1Of	vectors,expression
R10790	T13362	T13363	arg1Of	vectors,of
R10791	T13364	T13365	arg1Of	HA-p65,and
R10792	T13365	T13363	arg2Of	and,of
R10793	T13366	T13365	arg2Of	FLAG-Tat,and
R10794	T13368	T13367	arg1Of	and,","
R10795	T13371	T13369	arg1Of	Tat,24 h
R10796	T13371	T13370	arg1Of	Tat,later
R10797	T13371	T13372	arg1Of	Tat,was
R10798	T13371	T13373	arg2Of	Tat,immunoprecipitated
R10799	T13373	T13368	arg2Of	immunoprecipitated,and
R10800	T13373	T13372	arg2Of	immunoprecipitated,was
R10801	T13373	T13374	arg1Of	immunoprecipitated,from
R10802	T13376	T13374	arg2Of	extracts,from
R10803	T13376	T13375	arg1Of	extracts,cell
R10804	T13379	T13377	arg1Of	protein,The
R10805	T13379	T13378	arg1Of	protein,p65
R10806	T13379	T13380	arg1Of	protein,was
R10807	T13379	T13381	arg2Of	protein,coimmunoprecipitated
R10808	T13381	T13380	arg2Of	coimmunoprecipitated,was
R10809	T13381	T13382	arg1Of	coimmunoprecipitated,with
R10810	T13381	T13410	arg1Of	coimmunoprecipitated,at
R10811	T13381	T13413	arg1Of	coimmunoprecipitated,","
R10812	T13381	T13414	arg1Of	coimmunoprecipitated,while
R10813	T13384	T13383	arg1Of	Tat,wild-type
R10814	T13384	T13385	arg1Of	Tat,or
R10815	T13385	T13390	arg1Of	or,","
R10816	T13387	T13386	arg1Of	mutants,the
R10817	T13389	T13385	arg2Of	T,or
R10818	T13389	T13387	arg1Of	T,mutants
R10819	T13389	T13388	arg1Of	T,Tat
R10820	T13390	T13396	arg1Of	",",","
R10821	T13393	T13392	arg2Of	"23,24",(
R10822	T13394	T13392	arg3Of	),(
R10823	T13395	T13390	arg2Of	A,","
R10824	T13395	T13391	arg1Of	A,N
R10825	T13395	T13392	arg1Of	A,(
R10826	T13396	T13403	arg1Of	",",and
R10827	T13400	T13399	arg2Of	"50,51",(
R10828	T13401	T13399	arg3Of	),(
R10829	T13402	T13396	arg2Of	A,","
R10830	T13402	T13397	arg1Of	A,Tat
R10831	T13402	T13398	arg1Of	A,K
R10832	T13402	T13399	arg1Of	A,(
R10833	T13403	T13382	arg2Of	and,with
R10834	T13405	T13404	arg1Of	R,Tat
R10835	T13405	T13406	arg1Of	R,(
R10836	T13407	T13406	arg2Of	49–57,(
R10837	T13408	T13406	arg3Of	),(
R10838	T13409	T13403	arg2Of	A,and
R10839	T13409	T13405	arg1Of	A,R
R10840	T13412	T13410	arg2Of	levels,at
R10841	T13412	T13411	arg1Of	levels,similar
R10842	T13417	T13415	arg1Of	decrease,a
R10843	T13417	T13416	arg1Of	decrease,significant
R10844	T13417	T13418	arg1Of	decrease,in
R10845	T13417	T13420	arg1Of	decrease,was
R10846	T13417	T13421	arg2Of	decrease,observed
R10847	T13419	T13418	arg2Of	coimmunoprecipitation,in
R10848	T13421	T13414	arg2Of	observed,while
R10849	T13421	T13420	arg2Of	observed,was
R10850	T13421	T13422	arg1Of	observed,for
R10851	T13426	T13425	arg2Of	"22,25,27",(
R10852	T13427	T13425	arg3Of	),(
R10853	T13428	T13422	arg2Of	A,for
R10854	T13428	T13423	arg1Of	A,Tat
R10855	T13428	T13424	arg1Of	A,C
R10856	T13428	T13425	arg1Of	A,(
R10857	T13428	T13429	arg1Of	A,(
R10858	T13431	T13429	arg2Of	4A,(
R10859	T13431	T13430	arg1Of	4A,Figure
R10860	T13432	T13429	arg3Of	),(
R10861	T13435	T13442	arg1Of	GST-pull,showed
R10862	T13436	T13437	arg1Of	down,of
R10863	T13441	T13436	arg2Of	proteins,down
R10864	T13441	T13438	arg1Of	proteins,in
R10865	T13441	T13439	arg1Of	proteins,vitro
R10866	T13441	T13440	arg2Of	proteins,translated
R10867	T13442	T13433	arg1Of	showed,Consistently
R10868	T13442	T13434	arg1Of	showed,","
R10869	T13442	T13436	arg1Of	showed,down
R10870	T13445	T13444	arg1Of	binding,direct
R10871	T13445	T13446	arg1Of	binding,of
R10872	T13445	T13448	arg1Of	binding,to
R10873	T13445	T13452	arg1Of	binding,or
R10874	T13447	T13446	arg2Of	p65,of
R10875	T13450	T13448	arg2Of	Tat,to
R10876	T13450	T13449	arg1Of	Tat,wild-type
R10877	T13452	T13451	arg1Of	or,","
R10878	T13452	T13478	arg1Of	or,and
R10879	T13454	T13453	arg1Of	mutants,the
R10880	T13456	T13455	arg1Of	T,Tat
R10881	T13456	T13457	arg1Of	T,","
R10882	T13457	T13463	arg1Of	",",","
R10883	T13460	T13459	arg2Of	"23,24",(
R10884	T13461	T13459	arg3Of	),(
R10885	T13462	T13457	arg2Of	A,","
R10886	T13462	T13458	arg1Of	A,N
R10887	T13462	T13459	arg1Of	A,(
R10888	T13463	T13470	arg1Of	",",and
R10889	T13467	T13466	arg2Of	"50,51",(
R10890	T13468	T13466	arg3Of	),(
R10891	T13469	T13463	arg2Of	A,","
R10892	T13469	T13464	arg1Of	A,Tat
R10893	T13469	T13465	arg1Of	A,K
R10894	T13469	T13466	arg1Of	A,(
R10895	T13470	T13452	arg2Of	and,or
R10896	T13470	T13454	arg1Of	and,mutants
R10897	T13472	T13471	arg1Of	R,Tat
R10898	T13472	T13473	arg1Of	R,(
R10899	T13474	T13473	arg2Of	49–57,(
R10900	T13475	T13473	arg3Of	),(
R10901	T13476	T13470	arg2Of	A,and
R10902	T13476	T13472	arg1Of	A,R
R10903	T13478	T13442	arg2Of	and,showed
R10904	T13478	T13443	arg1Of	and,the
R10905	T13478	T13477	arg1Of	and,","
R10906	T13479	T13478	arg2Of	lack,and
R10907	T13479	T13480	arg1Of	lack,of
R10908	T13481	T13480	arg2Of	binding,of
R10909	T13481	T13482	arg1Of	binding,to
R10910	T13486	T13485	arg2Of	"22,25,27",(
R10911	T13487	T13485	arg3Of	),(
R10912	T13488	T13482	arg2Of	A,to
R10913	T13488	T13483	arg1Of	A,Tat
R10914	T13488	T13484	arg1Of	A,C
R10915	T13488	T13485	arg1Of	A,(
R10916	T13488	T13489	arg1Of	A,(
R10917	T13491	T13489	arg2Of	4B,(
R10918	T13491	T13490	arg1Of	4B,Figure
R10919	T13492	T13489	arg3Of	),(
R10920	T13494	T13493	arg1Of	results,These
R10921	T13494	T13495	arg1Of	results,indicated
R10922	T13497	T13498	arg1Of	Tat,associated
R10923	T13498	T13495	arg2Of	associated,indicated
R10924	T13498	T13496	arg1Of	associated,that
R10925	T13498	T13499	arg1Of	associated,with
R10926	T13500	T13501	arg1Of	p65,through
R10927	T13500	T13505	arg1Of	p65,and
R10928	T13504	T13501	arg2Of	domain,through
R10929	T13504	T13502	arg1Of	domain,the
R10930	T13504	T13503	arg1Of	domain,cysteine-rich
R10931	T13505	T13499	arg2Of	and,with
R10932	T13506	T13505	arg2Of	ruled-out,and
R10933	T13508	T13506	arg2Of	requirement,ruled-out
R10934	T13508	T13507	arg1Of	requirement,the
R10935	T13508	T13509	arg1Of	requirement,of
R10936	T13510	T13509	arg2Of	bridging,of
R10937	T13510	T13512	modOf	bridging,to
R10938	T13511	T13510	arg2Of	proteins,bridging
R10939	T13513	T13512	arg1Of	mediate,to
R10940	T13515	T13513	arg2Of	interaction,mediate
R10941	T13515	T13514	arg1Of	interaction,such
R10942	T13517	T13516	arg1Of	identify,To
R10943	T13520	T13517	arg2Of	domain,identify
R10944	T13520	T13518	arg1Of	domain,the
R10945	T13520	T13519	arg1Of	domain,p65
R10946	T13520	T13521	arg2Of	domain,required
R10947	T13521	T13522	arg1Of	required,for
R10948	T13523	T13522	arg2Of	binding,for
R10949	T13523	T13524	arg1Of	binding,to
R10950	T13525	T13524	arg2Of	Tat,to
R10951	T13528	T13527	arg1Of	cells,HeLa
R10952	T13528	T13529	arg1Of	cells,were
R10953	T13528	T13530	arg2Of	cells,transfected
R10954	T13530	T13516	modOf	transfected,To
R10955	T13530	T13526	arg1Of	transfected,","
R10956	T13530	T13529	arg2Of	transfected,were
R10957	T13530	T13531	arg1Of	transfected,with
R10958	T13532	T13531	arg2Of	FLAG-Tat,with
R10959	T13532	T13534	arg1Of	FLAG-Tat,with
R10960	T13532	T13561	arg1Of	FLAG-Tat,(
R10961	T13534	T13533	arg1Of	with,together
R10962	T13536	T13535	arg1Of	mutants,HA-p65
R10963	T13536	T13537	arg1Of	mutants,","
R10964	T13536	T13538	arg1Of	mutants,which
R10965	T13536	T13539	arg1Of	mutants,were
R10966	T13536	T13540	arg2Of	mutants,deleted
R10967	T13536	T13552	arg1Of	mutants,or
R10968	T13540	T13539	arg2Of	deleted,were
R10969	T13540	T13541	arg1Of	deleted,of
R10970	T13544	T13541	arg2Of	domain,of
R10971	T13544	T13542	arg1Of	domain,the
R10972	T13544	T13543	arg1Of	domain,transactivation
R10973	T13544	T13545	arg1Of	domain,[
R10974	T13546	T13545	arg2Of	p65ΔC,[
R10975	T13546	T13547	arg1Of	p65ΔC,(
R10976	T13548	T13547	arg2Of	1–318,(
R10977	T13549	T13547	arg3Of	),(
R10978	T13550	T13545	arg3Of	],[
R10979	T13552	T13534	arg2Of	or,with
R10980	T13552	T13551	arg1Of	or,","
R10981	T13554	T13552	arg2Of	RHD,or
R10982	T13554	T13553	arg1Of	RHD,the
R10983	T13554	T13555	arg1Of	RHD,[
R10984	T13556	T13555	arg2Of	p65ΔN,[
R10985	T13556	T13557	arg1Of	p65ΔN,(
R10986	T13558	T13557	arg2Of	122–551,(
R10987	T13559	T13557	arg3Of	),(
R10988	T13560	T13555	arg3Of	],[
R10989	T13563	T13561	arg2Of	4C,(
R10990	T13563	T13562	arg1Of	4C,Figure
R10991	T13564	T13561	arg3Of	),(
R10992	T13565	T13566	arg1Of	Tat,coimmunoprecipitated
R10993	T13566	T13567	arg1Of	coimmunoprecipitated,with
R10994	T13566	T13575	arg1Of	coimmunoprecipitated,with
R10995	T13566	T13584	arg1Of	coimmunoprecipitated,","
R10996	T13566	T13585	modOf	coimmunoprecipitated,indicating
R10997	T13567	T13573	arg1Of	with,and
R10998	T13568	T13567	arg2Of	p65ΔC,with
R10999	T13568	T13569	arg1Of	p65ΔC,(
R11000	T13570	T13569	arg2Of	1–318,(
R11001	T13571	T13569	arg3Of	),(
R11002	T13573	T13572	arg1Of	and,","
R11003	T13575	T13573	arg2Of	with,and
R11004	T13575	T13574	arg1Of	with,not
R11005	T13576	T13575	arg2Of	p65ΔN,with
R11006	T13576	T13577	arg1Of	p65ΔN,(
R11007	T13576	T13580	arg1Of	p65ΔN,(
R11008	T13578	T13577	arg2Of	122–551,(
R11009	T13579	T13577	arg3Of	),(
R11010	T13582	T13580	arg2Of	4D,(
R11011	T13582	T13581	arg1Of	4D,Figure
R11012	T13583	T13580	arg3Of	),(
R11013	T13588	T13587	arg1Of	RHD,the
R11014	T13588	T13589	arg1Of	RHD,of
R11015	T13588	T13591	arg1Of	RHD,was
R11016	T13588	T13592	arg2Of	RHD,involved
R11017	T13590	T13589	arg2Of	p65,of
R11018	T13592	T13585	arg2Of	involved,indicating
R11019	T13592	T13586	arg1Of	involved,that
R11020	T13592	T13591	arg2Of	involved,was
R11021	T13592	T13593	arg1Of	involved,in
R11022	T13596	T13593	arg2Of	interaction,in
R11023	T13596	T13594	arg1Of	interaction,the
R11024	T13596	T13595	arg1Of	interaction,physical
R11025	T13596	T13597	arg1Of	interaction,with
R11026	T13598	T13597	arg2Of	Tat,with
R11027	T13600	T13599	arg1Of	address,To
R11028	T13602	T13600	arg2Of	effect,address
R11029	T13602	T13601	arg1Of	effect,the
R11030	T13602	T13603	arg1Of	effect,of
R11031	T13602	T13605	arg1Of	effect,on
R11032	T13604	T13603	arg2Of	Tat,of
R11033	T13609	T13605	arg2Of	affinity,on
R11034	T13609	T13606	arg1Of	affinity,the
R11035	T13609	T13607	arg1Of	affinity,p65
R11036	T13609	T13608	arg1Of	affinity,DNA-binding
R11037	T13614	T13600	arg1Of	protein,address
R11038	T13614	T13611	arg2Of	protein,purified
R11039	T13614	T13612	arg1Of	protein,recombinant
R11040	T13614	T13613	arg1Of	protein,p65
R11041	T13614	T13615	arg1Of	protein,was
R11042	T13614	T13616	arg2Of	protein,incubated
R11043	T13616	T13615	arg2Of	incubated,was
R11044	T13616	T13617	arg1Of	incubated,with
R11045	T13616	T13636	arg1Of	incubated,and
R11046	T13620	T13617	arg2Of	oligonucleotide,with
R11047	T13620	T13618	arg1Of	oligonucleotide,32P-labeled-NF-κB
R11048	T13620	T13619	arg1Of	oligonucleotide,double-stranded
R11049	T13620	T13621	arg1Of	oligonucleotide,in
R11050	T13622	T13623	arg1Of	presence,or
R11051	T13623	T13621	arg2Of	or,in
R11052	T13623	T13625	arg1Of	or,of
R11053	T13624	T13623	arg2Of	absence,or
R11054	T13627	T13626	arg1Of	Tat,wild-type
R11055	T13627	T13628	arg1Of	Tat,or
R11056	T13628	T13625	arg2Of	or,of
R11057	T13632	T13631	arg2Of	"22,25,27",(
R11058	T13633	T13631	arg3Of	),(
R11059	T13634	T13628	arg2Of	A,or
R11060	T13634	T13629	arg1Of	A,Tat
R11061	T13634	T13630	arg1Of	A,C
R11062	T13634	T13631	arg1Of	A,(
R11063	T13636	T13599	modOf	and,To
R11064	T13636	T13610	arg1Of	and,","
R11065	T13636	T13635	arg1Of	and,","
R11066	T13638	T13637	arg1Of	DNA-binding,p65
R11067	T13638	T13639	arg1Of	DNA-binding,was
R11068	T13638	T13640	arg2Of	DNA-binding,analysed
R11069	T13640	T13636	arg2Of	analysed,and
R11070	T13640	T13639	arg2Of	analysed,was
R11071	T13642	T13640	arg1Of	EMSA,analysed
R11072	T13642	T13641	arg2Of	EMSA,by
R11073	T13645	T13644	arg1Of	Tat,wild-type
R11074	T13645	T13647	arg1Of	Tat,increased
R11075	T13647	T13643	arg2Of	increased,Whereas
R11076	T13647	T13646	arg1Of	increased,significantly
R11077	T13649	T13647	arg2Of	binding,increased
R11078	T13649	T13648	arg1Of	binding,the
R11079	T13649	T13650	arg1Of	binding,of
R11080	T13649	T13652	arg1Of	binding,to
R11081	T13651	T13650	arg2Of	p65,of
R11082	T13653	T13652	arg2Of	DNA,to
R11083	T13660	T13659	arg2Of	"22,25,27",(
R11084	T13661	T13659	arg3Of	),(
R11085	T13662	T13655	arg1Of	A,the
R11086	T13662	T13656	arg1Of	A,mutant
R11087	T13662	T13657	arg1Of	A,Tat
R11088	T13662	T13658	arg1Of	A,C
R11089	T13662	T13659	arg1Of	A,(
R11090	T13662	T13663	arg1Of	A,","
R11091	T13662	T13671	arg1Of	A,was
R11092	T13662	T13672	arg1Of	A,ineffective
R11093	T13664	T13663	arg2Of	lacking,","
R11094	T13667	T13664	arg2Of	site,lacking
R11095	T13667	T13665	arg1Of	site,the
R11096	T13667	T13666	arg1Of	site,binding
R11097	T13667	T13668	arg1Of	site,for
R11098	T13669	T13668	arg2Of	p65,for
R11099	T13671	T13643	arg1Of	was,Whereas
R11100	T13671	T13654	arg1Of	was,","
R11101	T13671	T13670	arg1Of	was,","
R11102	T13672	T13671	arg2Of	ineffective,was
R11103	T13672	T13673	arg1Of	ineffective,(
R11104	T13675	T13673	arg2Of	4E,(
R11105	T13675	T13674	arg1Of	4E,Figure
R11106	T13676	T13673	arg3Of	),(
R11107	T13679	T13677	arg1Of	binding,The
R11108	T13679	T13678	arg1Of	binding,p65
R11109	T13679	T13680	arg1Of	binding,to
R11110	T13679	T13682	arg1Of	binding,was
R11111	T13679	T13683	arg2Of	binding,evaluated
R11112	T13681	T13680	arg2Of	DNA,to
R11113	T13683	T13682	arg2Of	evaluated,was
R11114	T13685	T13683	arg1Of	competition,evaluated
R11115	T13685	T13684	arg2Of	competition,by
R11116	T13685	T13686	arg1Of	competition,with
R11117	T13688	T13686	arg2Of	amounts,with
R11118	T13688	T13687	arg1Of	amounts,increasing
R11119	T13688	T13689	arg1Of	amounts,of
R11120	T13693	T13689	arg2Of	oligonucleotide,of
R11121	T13693	T13690	arg1Of	oligonucleotide,cold
R11122	T13693	T13691	arg1Of	oligonucleotide,competitor
R11123	T13693	T13692	arg1Of	oligonucleotide,NF-κB
R11124	T13693	T13694	arg1Of	oligonucleotide,","
R11125	T13693	T13695	arg1Of	oligonucleotide,which
R11126	T13693	T13696	arg1Of	oligonucleotide,showed
R11127	T13698	T13700	arg1Of	Tat,enhanced
R11128	T13700	T13696	arg2Of	enhanced,showed
R11129	T13700	T13697	arg1Of	enhanced,that
R11130	T13700	T13699	arg1Of	enhanced,significantly
R11131	T13704	T13700	arg2Of	binding,enhanced
R11132	T13704	T13701	arg1Of	binding,the
R11133	T13704	T13702	arg1Of	binding,p65
R11134	T13704	T13703	arg1Of	binding,affinity
R11135	T13704	T13705	arg1Of	binding,to
R11136	T13704	T13707	arg1Of	binding,(
R11137	T13706	T13705	arg2Of	DNA,to
R11138	T13709	T13708	arg1Of	4E,Figure
R11139	T13709	T13710	arg1Of	4E,and
R11140	T13710	T13707	arg2Of	and,(
R11141	T13711	T13710	arg2Of	F,and
R11142	T13712	T13707	arg3Of	),(
R12187	T15237	T15238	arg1Of	Tat,activates
R12188	T15237	T15246	arg1Of	Tat,occupies
R12189	T15237	T15251	arg1Of	Tat,promotes
R12190	T15238	T15245	arg1Of	activates,","
R12191	T15241	T15238	arg2Of	expression,activates
R12192	T15241	T15239	arg1Of	expression,the
R12193	T15241	T15240	arg1Of	expression,p65-dependent
R12194	T15241	T15242	arg1Of	expression,of
R12195	T15244	T15242	arg2Of	genes,of
R12196	T15244	T15243	arg1Of	genes,NF-κB-responsive
R12197	T15245	T15250	arg1Of	",",and
R12198	T15246	T15245	arg2Of	occupies,","
R12199	T15249	T15246	arg2Of	enhancers,occupies
R12200	T15249	T15247	arg1Of	enhancers,the
R12201	T15249	T15248	arg1Of	enhancers,NF-κB
R12202	T15251	T15250	arg2Of	promotes,and
R12203	T15254	T15251	arg2Of	recruitment,promotes
R12204	T15254	T15252	arg1Of	recruitment,the
R12205	T15254	T15253	arg1Of	recruitment,p65
R12206	T15254	T15255	arg1Of	recruitment,with
R12207	T15257	T15255	arg2Of	displacement,with
R12208	T15257	T15256	arg1Of	displacement,IκB-α
R12209	T15258	T15259	arg1Of	We,analysed
R12210	T15259	T15271	arg1Of	analysed,vivo
R12211	T15261	T15259	arg2Of	action,analysed
R12212	T15261	T15260	arg1Of	action,the
R12213	T15261	T15262	arg1Of	action,of
R12214	T15261	T15264	arg1Of	action,on
R12215	T15263	T15262	arg2Of	Tat,of
R12216	T15266	T15264	arg2Of	expression,on
R12217	T15266	T15265	arg1Of	expression,the
R12218	T15266	T15267	arg1Of	expression,of
R12219	T15269	T15267	arg2Of	genes,of
R12220	T15269	T15268	arg1Of	genes,NF-κB-responsive
R12221	T15271	T15270	arg1Of	vivo,in
R12222	T15274	T15272	arg2Of	end,To
R12223	T15274	T15273	arg1Of	end,this
R12224	T15277	T15276	arg1Of	cells,HeLa
R12225	T15277	T15278	arg1Of	cells,were
R12226	T15277	T15279	arg2Of	cells,transfected
R12227	T15279	T15272	arg1Of	transfected,To
R12228	T15279	T15275	arg1Of	transfected,","
R12229	T15279	T15278	arg2Of	transfected,were
R12230	T15279	T15280	arg1Of	transfected,with
R12231	T15281	T15282	arg1Of	FLAG-Tat,","
R12232	T15282	T15289	arg1Of	",",or
R12233	T15286	T15285	arg2Of	"22,25,27",(
R12234	T15287	T15285	arg3Of	),(
R12235	T15288	T15282	arg2Of	A,","
R12236	T15288	T15283	arg1Of	A,FLAG-Tat
R12237	T15288	T15284	arg1Of	A,C
R12238	T15288	T15285	arg1Of	A,(
R12239	T15289	T15297	arg1Of	or,and
R12240	T15291	T15290	arg1Of	R,FLAG-Tat
R12241	T15291	T15292	arg1Of	R,(
R12242	T15293	T15292	arg2Of	49–57,(
R12243	T15294	T15292	arg3Of	),(
R12244	T15295	T15289	arg2Of	A,or
R12245	T15295	T15291	arg1Of	A,R
R12246	T15297	T15280	arg2Of	and,with
R12247	T15297	T15296	arg1Of	and,","
R12248	T15298	T15297	arg2Of	48 h,and
R12249	T15298	T15308	modOf	48 h,was
R12250	T15301	T15299	arg1Of	expression,later
R12251	T15301	T15300	arg1Of	expression,the
R12252	T15301	T15302	arg1Of	expression,of
R12253	T15301	T15308	arg1Of	expression,was
R12254	T15301	T15309	arg2Of	expression,analysed
R12255	T15304	T15302	arg2Of	number,of
R12256	T15304	T15303	arg1Of	number,a
R12257	T15304	T15305	arg1Of	number,of
R12258	T15307	T15305	arg2Of	genes,of
R12259	T15307	T15306	arg1Of	genes,NF-κB-dependent
R12260	T15309	T15308	arg2Of	analysed,was
R12261	T15312	T15309	arg1Of	PCR,analysed
R12262	T15312	T15310	arg2Of	PCR,by
R12263	T15312	T15311	arg1Of	PCR,real-time
R12264	T15313	T15315	arg1Of	Tat,increased
R12265	T15315	T15314	arg1Of	increased,significantly
R12266	T15315	T15328	arg1Of	increased,","
R12267	T15315	T15329	arg1Of	increased,while
R12268	T15317	T15315	arg2Of	expression,increased
R12269	T15317	T15316	arg1Of	expression,the
R12270	T15317	T15318	arg1Of	expression,of
R12271	T15319	T15320	arg1Of	MIP-1α,","
R12272	T15320	T15322	arg1Of	",",","
R12273	T15321	T15320	arg2Of	CSF3,","
R12274	T15322	T15324	arg1Of	",",","
R12275	T15323	T15322	arg2Of	LTA,","
R12276	T15324	T15326	arg1Of	",",and
R12277	T15325	T15324	arg2Of	NFKBIA,","
R12278	T15326	T15318	arg2Of	and,of
R12279	T15327	T15326	arg2Of	TLR2,and
R12280	T15335	T15334	arg2Of	"22,25,27",(
R12281	T15336	T15334	arg3Of	),(
R12282	T15337	T15332	arg1Of	A,Tat
R12283	T15337	T15333	arg1Of	A,C
R12284	T15337	T15334	arg1Of	A,(
R12285	T15337	T15338	arg1Of	A,and
R12286	T15338	T15330	arg1Of	and,the
R12287	T15338	T15331	arg1Of	and,mutants
R12288	T15338	T15345	arg1Of	and,were
R12289	T15338	T15346	arg1Of	and,ineffective
R12290	T15340	T15339	arg1Of	R,Tat
R12291	T15340	T15341	arg1Of	R,(
R12292	T15342	T15341	arg2Of	49–57,(
R12293	T15343	T15341	arg3Of	),(
R12294	T15344	T15338	arg2Of	A,and
R12295	T15344	T15340	arg1Of	A,R
R12296	T15345	T15329	arg2Of	were,while
R12297	T15346	T15345	arg2Of	ineffective,were
R12298	T15346	T15347	arg1Of	ineffective,(
R12299	T15349	T15347	arg2Of	5A–E,(
R12300	T15349	T15348	arg1Of	5A–E,Figure
R12301	T15350	T15347	arg3Of	),(
R12302	T15353	T15351	arg1Of	transactivation,The
R12303	T15353	T15352	arg1Of	transactivation,Tat-dependent
R12304	T15353	T15354	arg1Of	transactivation,of
R12305	T15353	T15358	arg1Of	transactivation,was
R12306	T15353	T15359	arg2Of	transactivation,abolished
R12307	T15357	T15354	arg2Of	genes,of
R12308	T15357	T15355	arg1Of	genes,the
R12309	T15357	T15356	arg1Of	genes,NF-κB-dependent
R12310	T15359	T15358	arg2Of	abolished,was
R12311	T15359	T15360	arg1Of	abolished,when
R12312	T15359	T15367	arg1Of	abolished,","
R12313	T15359	T15368	modOf	abolished,indicating
R12314	T15361	T15362	arg1Of	cells,were
R12315	T15361	T15363	arg2Of	cells,transfected
R12316	T15363	T15360	arg2Of	transfected,when
R12317	T15363	T15362	arg2Of	transfected,were
R12318	T15363	T15364	arg1Of	transfected,with
R12319	T15366	T15364	arg2Of	p65,with
R12320	T15366	T15365	arg1Of	p65,siRNA
R12321	T15373	T15370	arg1Of	activation,the
R12322	T15373	T15371	arg1Of	activation,Tat
R12323	T15373	T15372	arg1Of	activation,transcriptional
R12324	T15373	T15374	arg1Of	activation,was
R12325	T15373	T15375	arg2Of	activation,mediated
R12326	T15375	T15368	arg2Of	mediated,indicating
R12327	T15375	T15369	arg1Of	mediated,that
R12328	T15375	T15374	arg2Of	mediated,was
R12329	T15377	T15375	arg1Of	p65,mediated
R12330	T15377	T15376	arg2Of	p65,by
R12331	T15377	T15378	arg1Of	p65,(
R12332	T15380	T15378	arg2Of	5A–E,(
R12333	T15380	T15379	arg1Of	5A–E,Figure
R12334	T15381	T15378	arg3Of	),(
R12335	T15385	T15384	arg1Of	expression,the
R12336	T15385	T15386	arg1Of	expression,of
R12337	T15385	T15393	arg1Of	expression,was
R12338	T15385	T15394	arg2Of	expression,unaffected
R12339	T15389	T15387	arg1Of	genes,the
R12340	T15389	T15388	arg1Of	genes,NF-κB-independent
R12341	T15390	T15391	arg1Of	GAPDH,and
R12342	T15391	T15386	arg2Of	and,of
R12343	T15391	T15389	arg1Of	and,genes
R12344	T15392	T15391	arg2Of	ACTB,and
R12345	T15394	T15382	arg1Of	unaffected,Differently
R12346	T15394	T15383	arg1Of	unaffected,","
R12347	T15394	T15393	arg2Of	unaffected,was
R12348	T15394	T15395	arg1Of	unaffected,by
R12349	T15396	T15397	arg1Of	wild-type,and
R12350	T15397	T15395	arg2Of	and,by
R12351	T15397	T15400	arg1Of	and,(
R12352	T15399	T15397	arg2Of	mutants,and
R12353	T15399	T15398	arg1Of	mutants,Tat
R12354	T15402	T15401	arg1Of	5F,Figure
R12355	T15402	T15403	arg1Of	5F,and
R12356	T15403	T15400	arg2Of	and,(
R12357	T15404	T15403	arg2Of	G,and
R12358	T15405	T15400	arg3Of	),(
R12359	T15407	T15406	arg1Of	results,These
R12360	T15407	T15408	arg1Of	results,were
R12361	T15407	T15409	arg1Of	results,consistent
R12362	T15409	T15408	arg2Of	consistent,were
R12363	T15409	T15410	arg1Of	consistent,with
R12364	T15412	T15410	arg2Of	requirement,with
R12365	T15412	T15411	arg1Of	requirement,the
R12366	T15412	T15413	arg1Of	requirement,of
R12367	T15416	T15417	arg1Of	cysteine-rich,and
R12368	T15418	T15417	arg2Of	arginine-rich,and
R12369	T15419	T15413	arg2Of	domains,of
R12370	T15419	T15414	arg1Of	domains,both
R12371	T15419	T15415	arg1Of	domains,the
R12372	T15419	T15416	arg1Of	domains,cysteine-rich
R12373	T15419	T15418	arg1Of	domains,arginine-rich
R12374	T15419	T15420	arg1Of	domains,of
R12375	T15421	T15420	arg2Of	Tat,of
R12376	T15421	T15422	modOf	Tat,to
R12377	T15423	T15422	arg1Of	enhance,to
R12378	T15423	T15427	arg1Of	enhance,","
R12379	T15423	T15428	modOf	enhance,suggesting
R12380	T15426	T15423	arg2Of	activity,enhance
R12381	T15426	T15424	arg1Of	activity,the
R12382	T15426	T15425	arg1Of	activity,NF-κB
R12383	T15431	T15430	arg1Of	up-regulation,the
R12384	T15431	T15432	arg1Of	up-regulation,of
R12385	T15431	T15435	arg1Of	up-regulation,by
R12386	T15431	T15437	arg1Of	up-regulation,might
R12387	T15431	T15438	arg1Of	up-regulation,occur
R12388	T15434	T15432	arg2Of	genes,of
R12389	T15434	T15433	arg1Of	genes,NF-κB-responsive
R12390	T15436	T15435	arg2Of	Tat,by
R12391	T15438	T15428	arg2Of	occur,suggesting
R12392	T15438	T15429	arg1Of	occur,that
R12393	T15438	T15437	arg2Of	occur,might
R12394	T15438	T15439	arg1Of	occur,through
R12395	T15441	T15439	arg2Of	interaction,through
R12396	T15441	T15440	arg1Of	interaction,physical
R12397	T15441	T15442	arg1Of	interaction,of
R12398	T15441	T15446	arg1Of	interaction,with
R12399	T15445	T15442	arg2Of	protein,of
R12400	T15445	T15443	arg1Of	protein,the
R12401	T15445	T15444	arg1Of	protein,viral
R12402	T15447	T15448	arg1Of	p65,and
R12403	T15448	T15446	arg2Of	and,with
R12404	T15449	T15448	arg2Of	IκB-α,and
R12405	T15450	T15451	arg1Of	MIP-1α,","
R12406	T15450	T15452	arg1Of	MIP-1α,which
R12407	T15450	T15453	arg1Of	MIP-1α,was
R12408	T15450	T15461	arg1Of	MIP-1α,encodes
R12409	T15456	T15455	arg1Of	activated,mostly
R12410	T15457	T15453	arg2Of	gene,was
R12411	T15457	T15454	arg1Of	gene,the
R12412	T15457	T15456	arg1Of	gene,activated
R12413	T15457	T15458	arg1Of	gene,by
R12414	T15459	T15458	arg2Of	Tat,by
R12415	T15461	T15460	arg1Of	encodes,","
R12416	T15461	T15462	arg1Of	encodes,for
R12417	T15464	T15462	arg2Of	chemokine,for
R12418	T15464	T15463	arg1Of	chemokine,a
R12419	T15464	T15465	arg1Of	chemokine,that
R12420	T15464	T15466	arg1Of	chemokine,promotes
R12421	T15468	T15466	arg2Of	recruitment,promotes
R12422	T15468	T15467	arg1Of	recruitment,the
R12423	T15468	T15469	arg1Of	recruitment,of
R12424	T15471	T15469	arg2Of	cells,of
R12425	T15471	T15470	arg1Of	cells,pro-inflammatory
R12426	T15471	T15472	arg1Of	cells,(
R12427	T15473	T15472	arg2Of	56,(
R12428	T15474	T15472	arg3Of	),(
R12429	T15478	T15476	arg2Of	findings,with
R12430	T15478	T15477	arg1Of	findings,our
R12431	T15481	T15480	arg1Of	expression,MIP-1α
R12432	T15481	T15482	arg1Of	expression,was
R12433	T15481	T15483	arg2Of	expression,increased
R12434	T15483	T15475	arg1Of	increased,Consistently
R12435	T15483	T15476	arg1Of	increased,with
R12436	T15483	T15479	arg1Of	increased,","
R12437	T15483	T15482	arg2Of	increased,was
R12438	T15483	T15484	arg1Of	increased,in
R12439	T15483	T15496	arg1Of	increased,;
R12440	T15486	T15485	arg1Of	cells,glial
R12441	T15486	T15487	arg1Of	cells,and
R12442	T15487	T15484	arg2Of	and,in
R12443	T15489	T15487	arg2Of	nodes,and
R12444	T15489	T15488	arg1Of	nodes,lymph
R12445	T15489	T15490	arg1Of	nodes,of
R12446	T15492	T15490	arg2Of	patients,of
R12447	T15492	T15491	arg1Of	patients,AIDS
R12448	T15492	T15493	arg1Of	patients,(
R12449	T15494	T15493	arg2Of	"57,58",(
R12450	T15495	T15493	arg3Of	),(
R12451	T15500	T15499	arg1Of	production,MIP-1α
R12452	T15500	T15501	arg1Of	production,was
R12453	T15500	T15502	arg2Of	production,induced
R12454	T15502	T15496	arg2Of	induced,;
R12455	T15502	T15497	arg1Of	induced,moreover
R12456	T15502	T15498	arg1Of	induced,","
R12457	T15502	T15501	arg2Of	induced,was
R12458	T15505	T15504	arg1Of	infection,HIV-1
R12459	T15505	T15506	arg1Of	infection,(
R12460	T15505	T15509	arg1Of	infection,and
R12461	T15507	T15506	arg2Of	"59,60",(
R12462	T15508	T15506	arg3Of	),(
R12463	T15509	T15502	arg1Of	and,induced
R12464	T15509	T15503	arg2Of	and,by
R12465	T15511	T15509	arg2Of	protein,and
R12466	T15511	T15510	arg1Of	protein,Tat
R12467	T15511	T15512	arg1Of	protein,(
R12468	T15513	T15512	arg2Of	"57,61,62",(
R12469	T15514	T15512	arg3Of	),(
R12470	T15517	T15515	arg1Of	regulation,The
R12471	T15517	T15516	arg1Of	regulation,transcriptional
R12472	T15517	T15518	arg1Of	regulation,of
R12473	T15517	T15520	arg1Of	regulation,has
R12474	T15517	T15521	arg1Of	regulation,been
R12475	T15517	T15523	arg2Of	regulation,characterized
R12476	T15519	T15518	arg2Of	MIP-1α,of
R12477	T15523	T15520	arg2Of	characterized,has
R12478	T15523	T15521	arg2Of	characterized,been
R12479	T15523	T15522	arg1Of	characterized,poorly
R12480	T15525	T15524	arg1Of	analysis,Jaspar-based
R12481	T15525	T15526	arg1Of	analysis,(
R12482	T15525	T15531	arg1Of	analysis,predicted
R12483	T15527	T15526	arg2Of	http,(
R12484	T15528	T15526	arg3Of	:,(
R12485	T15529	T15530	arg1Of	//jaspar.genereg.net/,)
R12486	T15531	T15529	arg1Of	predicted,//jaspar.genereg.net/
R12487	T15535	T15531	arg2Of	enhancers,predicted
R12488	T15535	T15532	arg1Of	enhancers,three
R12489	T15535	T15533	arg1Of	enhancers,putative
R12490	T15535	T15534	arg1Of	enhancers,NF-κB
R12491	T15535	T15536	arg1Of	enhancers,in
R12492	T15535	T15545	arg1Of	enhancers,:
R12493	T15540	T15536	arg2Of	region,in
R12494	T15540	T15537	arg1Of	region,the
R12495	T15540	T15538	arg1Of	region,proximal
R12496	T15540	T15539	arg1Of	region,promoter
R12497	T15540	T15541	arg1Of	region,of
R12498	T15544	T15541	arg2Of	gene,of
R12499	T15544	T15542	arg1Of	gene,the
R12500	T15544	T15543	arg1Of	gene,MIP-1α
R12501	T15546	T15545	arg2Of	NF-κB1,:
R12502	T15546	T15547	arg1Of	NF-κB1,","
R12503	T15546	T15550	arg1Of	NF-κB1,;
R12504	T15549	T15547	arg2Of	nucleotides,","
R12505	T15549	T15548	arg1Of	nucleotides,−1023/−1014
R12506	T15551	T15550	arg2Of	NF-κB2,;
R12507	T15551	T15552	arg1Of	NF-κB2,","
R12508	T15551	T15555	arg1Of	NF-κB2,;
R12509	T15554	T15552	arg2Of	nucleotides,","
R12510	T15554	T15553	arg1Of	nucleotides,−661/−652
R12511	T15556	T15555	arg2Of	NF-κB3,;
R12512	T15556	T15557	arg1Of	NF-κB3,","
R12513	T15559	T15557	arg2Of	nucleotides,","
R12514	T15559	T15558	arg1Of	nucleotides,+370/+379
R12515	T15559	T15560	arg1Of	nucleotides,(
R12516	T15563	T15560	arg2Of	S3A,(
R12517	T15563	T15561	arg1Of	S3A,Supplementary
R12518	T15563	T15562	arg1Of	S3A,Figure
R12519	T15564	T15560	arg3Of	),(
R12520	T15566	T15565	arg1Of	validate,To
R12521	T15571	T15566	arg2Of	sites,validate
R12522	T15571	T15567	arg1Of	sites,the
R12523	T15571	T15568	arg2Of	sites,predicted
R12524	T15571	T15569	arg1Of	sites,NF-κB
R12525	T15571	T15570	arg1Of	sites,binding
R12526	T15571	T15572	arg1Of	sites,of
R12527	T15575	T15572	arg2Of	promoter,of
R12528	T15575	T15573	arg1Of	promoter,the
R12529	T15575	T15574	arg1Of	promoter,MIP-1α
R12530	T15577	T15566	arg1Of	we,validate
R12531	T15577	T15578	arg1Of	we,stimulated
R12532	T15577	T15590	arg1Of	we,measured
R12533	T15578	T15581	arg1Of	stimulated,with
R12534	T15578	T15589	arg1Of	stimulated,and
R12535	T15580	T15578	arg2Of	cells,stimulated
R12536	T15580	T15579	arg1Of	cells,HeLa
R12537	T15582	T15581	arg2Of	TNF-α,with
R12538	T15582	T15583	arg1Of	TNF-α,","
R12539	T15587	T15583	arg2Of	inducer,","
R12540	T15587	T15584	arg1Of	inducer,a
R12541	T15587	T15585	arg1Of	inducer,well-known
R12542	T15587	T15586	arg1Of	inducer,NF-κB
R12543	T15589	T15565	modOf	and,To
R12544	T15589	T15576	arg1Of	and,","
R12545	T15589	T15588	arg1Of	and,","
R12546	T15590	T15589	arg2Of	measured,and
R12547	T15593	T15591	arg1Of	levels,the
R12548	T15593	T15592	arg1Of	levels,expression
R12549	T15593	T15594	arg1Of	levels,of
R12550	T15593	T15600	arg1Of	levels,and
R12551	T15595	T15594	arg2Of	MIP-1α,of
R12552	T15595	T15596	arg1Of	MIP-1α,by
R12553	T15598	T15596	arg2Of	PCR,by
R12554	T15598	T15597	arg1Of	PCR,real-time
R12555	T15600	T15590	arg2Of	and,measured
R12556	T15600	T15599	arg1Of	and,","
R12557	T15603	T15600	arg2Of	occupancy,and
R12558	T15603	T15601	arg1Of	occupancy,the
R12559	T15603	T15602	arg1Of	occupancy,p65
R12560	T15603	T15604	arg1Of	occupancy,of
R12561	T15603	T15608	arg1Of	occupancy,by
R12562	T15607	T15604	arg2Of	sites,of
R12563	T15607	T15605	arg1Of	sites,the
R12564	T15607	T15606	arg1Of	sites,NF-κB
R12565	T15609	T15608	arg2Of	ChIP,by
R12566	T15610	T15611	arg1Of	TNF-α,activated
R12567	T15610	T15617	arg1Of	TNF-α,induced
R12568	T15610	T15628	arg1Of	TNF-α,affecting
R12569	T15611	T15616	arg1Of	activated,and
R12570	T15613	T15611	arg2Of	expression,activated
R12571	T15613	T15612	arg1Of	expression,the
R12572	T15613	T15614	arg1Of	expression,of
R12573	T15615	T15614	arg2Of	MIP-1α,of
R12574	T15617	T15616	arg2Of	induced,and
R12575	T15617	T15627	arg1Of	induced,without
R12576	T15620	T15617	arg2Of	recruitment,induced
R12577	T15620	T15618	arg1Of	recruitment,the
R12578	T15620	T15619	arg1Of	recruitment,p65
R12579	T15620	T15621	arg1Of	recruitment,to
R12580	T15624	T15621	arg2Of	site,to
R12581	T15624	T15622	arg1Of	site,the
R12582	T15624	T15623	arg1Of	site,NF-κB1
R12583	T15624	T15625	arg1Of	site,of
R12584	T15626	T15625	arg2Of	MIP-1α,of
R12585	T15628	T15627	arg2Of	affecting,without
R12586	T15630	T15628	arg2Of	occupancy,affecting
R12587	T15630	T15629	arg1Of	occupancy,the
R12588	T15630	T15631	arg1Of	occupancy,of
R12589	T15634	T15635	arg1Of	NF-κB2,and
R12590	T15636	T15635	arg2Of	NF-κB3,and
R12591	T15637	T15631	arg2Of	sites,of
R12592	T15637	T15632	arg1Of	sites,the
R12593	T15637	T15633	arg1Of	sites,putative
R12594	T15637	T15634	arg1Of	sites,NF-κB2
R12595	T15637	T15636	arg1Of	sites,NF-κB3
R12596	T15637	T15638	arg1Of	sites,(
R12597	T15641	T15639	arg1Of	S3B,Supplementary
R12598	T15641	T15640	arg1Of	S3B,Figure
R12599	T15641	T15642	arg1Of	S3B,and
R12600	T15642	T15638	arg2Of	and,(
R12601	T15643	T15642	arg2Of	S3C,and
R12602	T15644	T15638	arg3Of	),(
R12603	T15645	T15647	arg1Of	Tat,promoted
R12604	T15647	T15646	arg1Of	promoted,also
R12605	T15647	T15658	arg1Of	promoted,","
R12606	T15647	T15659	arg1Of	promoted,while
R12607	T15649	T15647	arg2Of	recruitment,promoted
R12608	T15649	T15648	arg1Of	recruitment,the
R12609	T15649	T15650	arg1Of	recruitment,of
R12610	T15649	T15652	arg1Of	recruitment,to
R12611	T15651	T15650	arg2Of	p65,of
R12612	T15655	T15652	arg2Of	site,to
R12613	T15655	T15653	arg1Of	site,the
R12614	T15655	T15654	arg1Of	site,NF-κB1
R12615	T15655	T15656	arg1Of	site,of
R12616	T15657	T15656	arg2Of	MIP-1α,of
R12617	T15660	T15661	arg1Of	it,did
R12618	T15660	T15663	arg1Of	it,affect
R12619	T15663	T15659	arg2Of	affect,while
R12620	T15663	T15661	arg2Of	affect,did
R12621	T15663	T15662	arg1Of	affect,not
R12622	T15666	T15667	arg1Of	NF-κB2,and
R12623	T15668	T15667	arg2Of	NF-κB3,and
R12624	T15669	T15663	arg2Of	sites,affect
R12625	T15669	T15664	arg1Of	sites,the
R12626	T15669	T15665	arg1Of	sites,putative
R12627	T15669	T15666	arg1Of	sites,NF-κB2
R12628	T15669	T15668	arg1Of	sites,NF-κB3
R12629	T15669	T15670	arg1Of	sites,(
R12630	T15673	T15670	arg2Of	S3D,(
R12631	T15673	T15671	arg1Of	S3D,Supplementary
R12632	T15673	T15672	arg1Of	S3D,Figure
R12633	T15674	T15670	arg3Of	),(
R12634	T15677	T15676	arg1Of	results,these
R12635	T15677	T15678	arg1Of	results,indicated
R12636	T15678	T15675	arg1Of	indicated,Altogether
R12637	T15682	T15680	arg1Of	site,the
R12638	T15682	T15681	arg1Of	site,NF-κB1
R12639	T15682	T15683	arg1Of	site,was
R12640	T15683	T15678	arg2Of	was,indicated
R12641	T15683	T15679	arg1Of	was,that
R12642	T15683	T15693	arg1Of	was,in
R12643	T15688	T15683	arg2Of	enhancer,was
R12644	T15688	T15684	arg1Of	enhancer,the
R12645	T15688	T15685	arg1Of	enhancer,only
R12646	T15688	T15686	arg1Of	enhancer,effective
R12647	T15688	T15687	arg1Of	enhancer,NF-κB
R12648	T15688	T15689	arg1Of	enhancer,of
R12649	T15692	T15689	arg2Of	promoter,of
R12650	T15692	T15690	arg1Of	promoter,the
R12651	T15692	T15691	arg1Of	promoter,MIP-1α
R12652	T15694	T15693	arg2Of	response,in
R12653	T15694	T15695	arg1Of	response,to
R12654	T15696	T15697	arg1Of	TNF-α,and
R12655	T15697	T15695	arg2Of	and,to
R12656	T15698	T15697	arg2Of	Tat,and
R12657	T15699	T15700	arg1Of	Similarly,to
R12658	T15701	T15700	arg2Of	MIP-1α,to
R12659	T15703	T15704	arg1Of	Tat,increased
R12660	T15704	T15699	arg1Of	increased,Similarly
R12661	T15704	T15702	arg1Of	increased,","
R12662	T15704	T15721	arg1Of	increased,","
R12663	T15704	T15722	arg1Of	increased,while
R12664	T15706	T15704	arg2Of	recruitment,increased
R12665	T15706	T15705	arg1Of	recruitment,the
R12666	T15706	T15707	arg1Of	recruitment,of
R12667	T15706	T15709	arg1Of	recruitment,to
R12668	T15706	T15713	arg1Of	recruitment,of
R12669	T15708	T15707	arg2Of	p65,of
R12670	T15712	T15709	arg2Of	enhancers,to
R12671	T15712	T15710	arg1Of	enhancers,the
R12672	T15712	T15711	arg1Of	enhancers,NF-κB
R12673	T15714	T15715	arg1Of	CSF3,","
R12674	T15715	T15717	arg1Of	",",","
R12675	T15716	T15715	arg2Of	LTA,","
R12676	T15717	T15719	arg1Of	",",and
R12677	T15718	T15717	arg2Of	NFKBIA,","
R12678	T15719	T15713	arg2Of	and,of
R12679	T15720	T15719	arg2Of	TLR2,and
R12680	T15723	T15724	arg1Of	it,was
R12681	T15723	T15725	arg1Of	it,ineffective
R12682	T15724	T15722	arg2Of	was,while
R12683	T15724	T15726	arg1Of	was,at
R12684	T15725	T15724	arg2Of	ineffective,was
R12685	T15728	T15726	arg2Of	promoters,at
R12686	T15728	T15727	arg1Of	promoters,the
R12687	T15728	T15729	arg1Of	promoters,of
R12688	T15730	T15731	arg1Of	GAPDH,and
R12689	T15731	T15729	arg2Of	and,of
R12690	T15731	T15733	arg1Of	and,(
R12691	T15732	T15731	arg2Of	ACTB,and
R12692	T15735	T15733	arg2Of	5H,(
R12693	T15735	T15734	arg1Of	5H,Figure
R12694	T15736	T15733	arg3Of	),(
R12695	T15738	T15737	arg2Of	ChIP,By
R12696	T15740	T15741	arg1Of	Tat,bound
R12697	T15741	T15737	arg1Of	bound,By
R12698	T15741	T15739	arg1Of	bound,","
R12699	T15741	T15742	arg1Of	bound,to
R12700	T15741	T15749	arg1Of	bound,to
R12701	T15741	T15755	arg1Of	bound,","
R12702	T15741	T15756	arg1Of	bound,with
R12703	T15741	T15768	arg1Of	bound,","
R12704	T15741	T15769	modOf	bound,suggesting
R12705	T15742	T15747	arg1Of	to,and
R12706	T15745	T15742	arg2Of	promoters,to
R12707	T15745	T15743	arg1Of	promoters,the
R12708	T15745	T15744	arg1Of	promoters,NF-κB-responsive
R12709	T15747	T15746	arg1Of	and,","
R12710	T15749	T15747	arg2Of	to,and
R12711	T15749	T15748	arg1Of	to,not
R12712	T15751	T15752	arg1Of	GAPDH,and
R12713	T15753	T15752	arg2Of	ACTB,and
R12714	T15754	T15749	arg2Of	promoters,to
R12715	T15754	T15750	arg1Of	promoters,the
R12716	T15754	T15751	arg1Of	promoters,GAPDH
R12717	T15754	T15753	arg1Of	promoters,ACTB
R12718	T15757	T15756	arg2Of	loss,with
R12719	T15757	T15758	arg1Of	loss,of
R12720	T15757	T15760	arg1Of	loss,following
R12721	T15759	T15758	arg2Of	binding,of
R12722	T15763	T15760	arg2Of	interference,following
R12723	T15763	T15761	arg1Of	interference,p65
R12724	T15763	T15762	arg1Of	interference,RNA
R12725	T15763	T15764	arg1Of	interference,(
R12726	T15766	T15764	arg2Of	5I,(
R12727	T15766	T15765	arg1Of	5I,Figure
R12728	T15767	T15764	arg3Of	),(
R12729	T15772	T15771	arg1Of	occupancy,Tat
R12730	T15772	T15773	arg1Of	occupancy,occurred
R12731	T15773	T15769	arg2Of	occurred,suggesting
R12732	T15773	T15770	arg1Of	occurred,that
R12733	T15773	T15774	arg1Of	occurred,via
R12734	T15776	T15774	arg2Of	interaction,via
R12735	T15776	T15775	arg1Of	interaction,p65
R12736	T15779	T15777	arg2Of	findings,As
R12737	T15779	T15778	arg1Of	findings,additional
R12738	T15781	T15782	arg1Of	IκB-α,was
R12739	T15781	T15783	arg1Of	IκB-α,chromatin-immunoprecipitated
R12740	T15782	T15784	arg1Of	was,at
R12741	T15782	T15788	arg1Of	was,in
R12742	T15782	T15793	arg1Of	was,and
R12743	T15783	T15782	arg2Of	chromatin-immunoprecipitated,was
R12744	T15787	T15784	arg2Of	enhancers,at
R12745	T15787	T15785	arg1Of	enhancers,the
R12746	T15787	T15786	arg1Of	enhancers,NF-κB
R12747	T15788	T15790	arg1Of	in,of
R12748	T15789	T15788	arg2Of	absence,in
R12749	T15791	T15788	arg3Of	Tat,in
R12750	T15793	T15777	arg1Of	and,As
R12751	T15793	T15780	arg1Of	and,","
R12752	T15793	T15792	arg1Of	and,","
R12753	T15794	T15795	arg1Of	it,was
R12754	T15794	T15797	arg2Of	it,removed
R12755	T15797	T15793	arg2Of	removed,and
R12756	T15797	T15795	arg2Of	removed,was
R12757	T15797	T15796	arg1Of	removed,significantly
R12758	T15797	T15798	arg1Of	removed,in
R12759	T15797	T15812	arg1Of	removed,","
R12760	T15797	T15813	modOf	removed,indicating
R12761	T15798	T15800	arg1Of	in,of
R12762	T15799	T15798	arg2Of	presence,in
R12763	T15801	T15803	arg1Of	Tat,or
R12764	T15803	T15798	arg3Of	or,in
R12765	T15803	T15802	arg1Of	or,","
R12766	T15804	T15803	arg2Of	following,or
R12767	T15807	T15804	arg2Of	interference,following
R12768	T15807	T15805	arg1Of	interference,IκB-α
R12769	T15807	T15806	arg1Of	interference,RNA
R12770	T15807	T15808	arg1Of	interference,(
R12771	T15810	T15808	arg2Of	5J,(
R12772	T15810	T15809	arg1Of	5J,Figure
R12773	T15811	T15808	arg3Of	),(
R12774	T15815	T15816	arg1Of	Tat,displaced
R12775	T15815	T15817	arg1Of	Tat,IκB-α
R12776	T15816	T15813	arg2Of	displaced,indicating
R12777	T15816	T15814	arg1Of	displaced,that
R12778	T15817	T15816	arg2Of	IκB-α,displaced
R12779	T15817	T15818	arg1Of	IκB-α,from
R12780	T15819	T15818	arg2Of	promoters,from
R14250	T17797	T17795	arg2Of	monocytes,In
R14251	T17797	T17796	arg1Of	monocytes,HIV-1-infected
R14252	T17798	T17799	arg1Of	Tat,sustains
R14253	T17798	T17804	arg1Of	Tat,promotes
R14254	T17798	T17811	arg1Of	Tat,interacting
R14255	T17799	T17803	arg1Of	sustains,and
R14256	T17802	T17799	arg2Of	activity,sustains
R14257	T17802	T17800	arg1Of	activity,the
R14258	T17802	T17801	arg1Of	activity,NF-κB
R14259	T17803	T17795	arg1Of	and,In
R14260	T17803	T17810	arg1Of	and,by
R14261	T17804	T17803	arg2Of	promotes,and
R14262	T17807	T17804	arg2Of	activation,promotes
R14263	T17807	T17805	arg1Of	activation,the
R14264	T17807	T17806	arg1Of	activation,transcriptional
R14265	T17807	T17808	arg1Of	activation,of
R14266	T17809	T17808	arg2Of	MIP-1α,of
R14267	T17811	T17810	arg2Of	interacting,by
R14268	T17811	T17812	arg1Of	interacting,with
R14269	T17813	T17814	arg1Of	IκB-α,and
R14270	T17814	T17812	arg2Of	and,with
R14271	T17815	T17814	arg2Of	p65,and
R14272	T17816	T17818	arg2Of	We,analysed
R14273	T17818	T17817	arg1Of	analysed,next
R14274	T17820	T17821	arg1Of	Tat,affected
R14275	T17821	T17818	arg1Of	affected,analysed
R14276	T17821	T17819	arg1Of	affected,whether
R14277	T17821	T17830	arg1Of	affected,in
R14278	T17824	T17822	arg1Of	activity,the
R14279	T17824	T17823	arg1Of	activity,NF-κB
R14280	T17824	T17825	arg1Of	activity,and
R14281	T17825	T17821	arg2Of	and,affected
R14282	T17827	T17825	arg2Of	expression,and
R14283	T17827	T17826	arg1Of	expression,the
R14284	T17827	T17828	arg1Of	expression,of
R14285	T17829	T17828	arg2Of	MIP-1α,of
R14286	T17832	T17830	arg2Of	course,in
R14287	T17832	T17831	arg1Of	course,the
R14288	T17832	T17833	arg1Of	course,of
R14289	T17835	T17833	arg2Of	infection,of
R14290	T17835	T17834	arg1Of	infection,HIV-1
R14291	T17837	T17836	arg1Of	detect,To
R14292	T17840	T17837	arg2Of	protein,detect
R14293	T17840	T17838	arg1Of	protein,the
R14294	T17840	T17839	arg1Of	protein,Tat
R14295	T17842	T17837	arg1Of	we,detect
R14296	T17842	T17843	arg1Of	we,produced
R14297	T17843	T17836	modOf	produced,To
R14298	T17843	T17841	arg1Of	produced,","
R14299	T17847	T17843	arg2Of	virions,produced
R14300	T17847	T17844	arg1Of	virions,HXB2
R14301	T17847	T17845	arg1Of	virions,Env-pseudotyped
R14302	T17847	T17846	arg1Of	virions,NL4-3.FLAG-Tat.R−E−
R14303	T17847	T17848	arg1Of	virions,","
R14304	T17847	T17849	arg1Of	virions,which
R14305	T17847	T17850	arg1Of	virions,were
R14306	T17847	T17851	arg2Of	virions,used
R14307	T17851	T17850	arg2Of	used,were
R14308	T17851	T17852	arg1Of	used,in
R14309	T17854	T17852	arg2Of	infection,in
R14310	T17854	T17853	arg1Of	infection,single-round
R14311	T17854	T17855	arg1Of	infection,of
R14312	T17856	T17855	arg2Of	U937,of
R14313	T17856	T17857	arg1Of	U937,","
R14314	T17862	T17857	arg2Of	line,","
R14315	T17862	T17858	arg1Of	line,a
R14316	T17862	T17859	arg1Of	line,human
R14317	T17862	T17860	arg1Of	line,monocytic
R14318	T17862	T17861	arg1Of	line,cell
R14319	T17863	T17864	arg1Of	Cells,were
R14320	T17863	T17865	arg2Of	Cells,transfected
R14321	T17863	T17873	arg1Of	Cells,left
R14322	T17863	T17876	arg1Of	Cells,modulate
R14323	T17863	T17883	arg2Of	Cells,analysed
R14324	T17865	T17866	arg1Of	transfected,with
R14325	T17865	T17872	arg1Of	transfected,or
R14326	T17868	T17867	arg1Of	Tat,siRNA
R14327	T17868	T17869	arg1Of	Tat,","
R14328	T17869	T17866	arg2Of	",",with
R14329	T17871	T17869	arg2Of	control,","
R14330	T17871	T17870	arg1Of	control,siRNA
R14331	T17872	T17881	arg1Of	or,and
R14332	T17873	T17872	arg2Of	left,or
R14333	T17874	T17873	arg2Of	untransfected,left
R14334	T17876	T17873	arg3Of	modulate,left
R14335	T17876	T17875	arg1Of	modulate,to
R14336	T17879	T17876	arg2Of	expression,modulate
R14337	T17879	T17877	arg1Of	expression,the
R14338	T17879	T17878	arg1Of	expression,Tat
R14339	T17881	T17864	arg2Of	and,were
R14340	T17881	T17880	arg1Of	and,","
R14341	T17883	T17881	arg2Of	analysed,and
R14342	T17883	T17882	arg1Of	analysed,then
R14343	T17883	T17884	arg1Of	analysed,for
R14344	T17886	T17884	arg2Of	kinetic,for
R14345	T17886	T17885	arg1Of	kinetic,the
R14346	T17886	T17887	arg1Of	kinetic,of
R14347	T17889	T17887	arg2Of	activation,of
R14348	T17889	T17888	arg1Of	activation,NF-κB
R14349	T17889	T17890	arg1Of	activation,following
R14350	T17892	T17890	arg2Of	infection,following
R14351	T17892	T17891	arg1Of	infection,viral
R14352	T17894	T17893	arg1Of	expression,The
R14353	T17894	T17895	arg1Of	expression,of
R14354	T17894	T17899	arg1Of	expression,was
R14355	T17894	T17900	arg2Of	expression,detected
R14356	T17896	T17897	arg1Of	Tat,and
R14357	T17897	T17895	arg2Of	and,of
R14358	T17898	T17897	arg2Of	p24,and
R14359	T17900	T17899	arg2Of	detected,was
R14360	T17900	T17901	arg1Of	detected,at
R14361	T17900	T17903	arg1Of	detected,to
R14362	T17900	T17906	arg1Of	detected,","
R14363	T17900	T17907	arg1Of	detected,while
R14364	T17902	T17901	arg2Of	3-,at
R14365	T17905	T17903	arg2Of	post-infection,to
R14366	T17905	T17904	arg1Of	post-infection,12-h
R14367	T17908	T17909	arg1Of	it,was
R14368	T17908	T17911	arg2Of	it,detected
R14369	T17911	T17907	arg2Of	detected,while
R14370	T17911	T17909	arg2Of	detected,was
R14371	T17911	T17910	arg1Of	detected,barely
R14372	T17911	T17912	arg1Of	detected,in
R14373	T17915	T17912	arg2Of	cells,in
R14374	T17915	T17913	arg1Of	cells,siRNA
R14375	T17915	T17914	arg1Of	cells,Tat-transfected
R14376	T17915	T17916	arg1Of	cells,(
R14377	T17918	T17916	arg2Of	6A,(
R14378	T17918	T17917	arg1Of	6A,Figure
R14379	T17919	T17916	arg3Of	),(
R14380	T17922	T17920	arg1Of	activity,The
R14381	T17922	T17921	arg1Of	activity,NF-κB
R14382	T17922	T17931	arg1Of	activity,was
R14383	T17922	T17932	arg2Of	activity,induced
R14384	T17922	T17955	arg1Of	activity,increased
R14385	T17925	T17924	arg2Of	measured,as
R14386	T17925	T17926	arg1Of	measured,by
R14387	T17929	T17926	arg2Of	binding,by
R14388	T17929	T17927	arg1Of	binding,p65
R14389	T17929	T17928	arg1Of	binding,DNA
R14390	T17932	T17931	arg2Of	induced,was
R14391	T17932	T17933	arg1Of	induced,at
R14392	T17932	T17936	arg1Of	induced,in
R14393	T17932	T17954	arg1Of	induced,and
R14394	T17935	T17933	arg2Of	post-infection,at
R14395	T17935	T17934	arg1Of	post-infection,3-h
R14396	T17938	T17937	arg1Of	Tat-positive,both
R14397	T17938	T17939	arg1Of	Tat-positive,(
R14398	T17938	T17946	arg1Of	Tat-positive,and
R14399	T17941	T17942	arg1Of	siRNA,and
R14400	T17942	T17939	arg2Of	and,(
R14401	T17942	T17940	arg1Of	and,no
R14402	T17944	T17942	arg2Of	control,and
R14403	T17944	T17943	arg1Of	control,siRNA
R14404	T17945	T17939	arg3Of	),(
R14405	T17946	T17936	arg2Of	and,in
R14406	T17948	T17946	arg2Of	cells,and
R14407	T17948	T17947	arg1Of	cells,Tat-negative
R14408	T17948	T17949	arg1Of	cells,(
R14409	T17951	T17949	arg2Of	Tat,(
R14410	T17951	T17950	arg1Of	Tat,siRNA
R14411	T17952	T17949	arg3Of	),(
R14412	T17954	T17923	arg1Of	and,","
R14413	T17954	T17924	arg1Of	and,as
R14414	T17954	T17930	arg1Of	and,","
R14415	T17954	T17953	arg1Of	and,","
R14416	T17955	T17954	arg2Of	increased,and
R14417	T17955	T17956	arg1Of	increased,at
R14418	T17955	T17959	arg1Of	increased,in
R14419	T17957	T17956	arg2Of	12 h,at
R14420	T17959	T17958	arg1Of	in,only
R14421	T17961	T17959	arg2Of	cells,in
R14422	T17961	T17960	arg1Of	cells,Tat-positive
R14423	T17961	T17962	arg1Of	cells,(
R14424	T17964	T17962	arg2Of	6B,(
R14425	T17964	T17963	arg1Of	6B,Figure
R14426	T17965	T17962	arg3Of	),(
R14427	T17966	T17967	arg1Of	Degradation,of
R14428	T17966	T17969	arg1Of	Degradation,was
R14429	T17966	T17970	arg2Of	Degradation,observed
R14430	T17966	T17976	arg1Of	Degradation,was
R14431	T17966	T17977	arg2Of	Degradation,followed
R14432	T17968	T17967	arg2Of	IκB-α,of
R14433	T17970	T17969	arg2Of	observed,was
R14434	T17970	T17971	arg1Of	observed,at
R14435	T17970	T17975	arg1Of	observed,and
R14436	T17973	T17971	arg2Of	post-infection,at
R14437	T17973	T17972	arg1Of	post-infection,3-h
R14438	T17975	T17974	arg1Of	and,","
R14439	T17977	T17975	arg2Of	followed,and
R14440	T17977	T17976	arg2Of	followed,was
R14441	T17977	T17984	arg1Of	followed,at
R14442	T17980	T17979	arg1Of	novo,de
R14443	T17981	T17977	arg1Of	synthesis,followed
R14444	T17981	T17978	arg2Of	synthesis,by
R14445	T17981	T17980	arg1Of	synthesis,novo
R14446	T17981	T17982	arg1Of	synthesis,of
R14447	T17983	T17982	arg2Of	IκB-α,of
R14448	T17985	T17984	arg2Of	12 h,at
R14449	T17985	T17986	arg1Of	12 h,independently
R14450	T17986	T17987	arg1Of	independently,of
R14451	T17990	T17987	arg2Of	presence,of
R14452	T17990	T17988	arg1Of	presence,the
R14453	T17990	T17989	arg1Of	presence,Tat
R14454	T17990	T17991	arg1Of	presence,(
R14455	T17993	T17991	arg2Of	6C,(
R14456	T17993	T17992	arg1Of	6C,Figure
R14457	T17994	T17991	arg3Of	),(
R14458	T17999	T17997	arg1Of	activity,the
R14459	T17999	T17998	arg1Of	activity,IKK
R14460	T17999	T18000	arg1Of	activity,was
R14461	T17999	T18001	arg2Of	activity,induced
R14462	T17999	T18006	arg1Of	activity,turned
R14463	T18001	T18000	arg2Of	induced,was
R14464	T18001	T18002	arg1Of	induced,at
R14465	T18001	T18005	arg1Of	induced,and
R14466	T18004	T18002	arg2Of	post-infection,at
R14467	T18004	T18003	arg1Of	post-infection,3-h
R14468	T18005	T17995	arg1Of	and,Consistently
R14469	T18005	T17996	arg1Of	and,","
R14470	T18006	T18005	arg2Of	turned,and
R14471	T18006	T18007	arg1Of	turned,off
R14472	T18006	T18008	arg1Of	turned,at
R14473	T18006	T18010	arg1Of	turned,independently
R14474	T18009	T18008	arg2Of	12 h,at
R14475	T18010	T18011	arg1Of	independently,of
R14476	T18014	T18011	arg2Of	presence,of
R14477	T18014	T18012	arg1Of	presence,the
R14478	T18014	T18013	arg1Of	presence,Tat
R14479	T18014	T18015	arg1Of	presence,(
R14480	T18017	T18015	arg2Of	6D,(
R14481	T18017	T18016	arg1Of	6D,Figure
R14482	T18018	T18015	arg3Of	),(
R14483	T18020	T18019	arg1Of	results,These
R14484	T18020	T18021	arg1Of	results,agreed
R14485	T18020	T18042	arg1Of	results,supported
R14486	T18021	T18022	arg1Of	agreed,with
R14487	T18021	T18040	arg1Of	agreed,and
R14488	T18024	T18022	arg2Of	kinetic,with
R14489	T18024	T18023	arg1Of	kinetic,the
R14490	T18024	T18025	arg1Of	kinetic,of
R14491	T18027	T18025	arg2Of	activation,of
R14492	T18027	T18026	arg1Of	activation,NF-κB
R14493	T18027	T18028	arg1Of	activation,in
R14494	T18031	T18028	arg2Of	infection,in
R14495	T18031	T18029	arg1Of	infection,single-round
R14496	T18031	T18030	arg1Of	infection,HIV-1
R14497	T18031	T18032	arg1Of	infection,of
R14498	T18034	T18032	arg2Of	cells,of
R14499	T18034	T18033	arg1Of	cells,Jurkat
R14500	T18034	T18035	arg1Of	cells,(
R14501	T18036	T18035	arg2Of	Figure,(
R14502	T18036	T18037	arg1Of	Figure,1
R14503	T18038	T18035	arg3Of	),(
R14504	T18040	T18039	arg1Of	and,","
R14505	T18042	T18040	arg2Of	supported,and
R14506	T18042	T18041	arg1Of	supported,further
R14507	T18044	T18042	arg2Of	requirement,supported
R14508	T18044	T18043	arg1Of	requirement,the
R14509	T18044	T18045	arg1Of	requirement,of
R14510	T18044	T18048	modOf	requirement,to
R14511	T18047	T18045	arg2Of	expression,of
R14512	T18047	T18046	arg1Of	expression,viral
R14513	T18049	T18048	arg1Of	counteract,to
R14514	T18049	T18053	arg1Of	counteract,off
R14515	T18052	T18049	arg2Of	turn,counteract
R14516	T18052	T18050	arg1Of	turn,the
R14517	T18052	T18051	arg1Of	turn,post-activation
R14518	T18053	T18054	arg1Of	off,of
R14519	T18055	T18054	arg2Of	NF-κB,of
R14520	T18059	T18056	arg2Of	cells,In
R14521	T18059	T18057	arg1Of	cells,HIV-1-infected
R14522	T18059	T18058	arg1Of	cells,U937
R14523	T18063	T18061	arg1Of	Tat,the
R14524	T18063	T18062	arg1Of	Tat,endogenous
R14525	T18063	T18064	arg1Of	Tat,was
R14526	T18063	T18065	arg2Of	Tat,coimmunoprecipitated
R14527	T18063	T18076	arg1Of	Tat,activated
R14528	T18065	T18064	arg2Of	coimmunoprecipitated,was
R14529	T18065	T18066	arg1Of	coimmunoprecipitated,with
R14530	T18065	T18075	arg1Of	coimmunoprecipitated,and
R14531	T18067	T18068	arg1Of	IκB-α,and
R14532	T18068	T18066	arg2Of	and,with
R14533	T18069	T18068	arg2Of	p65,and
R14534	T18069	T18070	arg1Of	p65,(
R14535	T18072	T18070	arg2Of	6E,(
R14536	T18072	T18071	arg1Of	6E,Figure
R14537	T18073	T18070	arg3Of	),(
R14538	T18075	T18056	arg1Of	and,In
R14539	T18075	T18060	arg1Of	and,","
R14540	T18075	T18074	arg1Of	and,","
R14541	T18076	T18075	arg2Of	activated,and
R14542	T18076	T18080	arg1Of	activated,in
R14543	T18076	T18084	arg1Of	activated,","
R14544	T18076	T18085	arg1Of	activated,as
R14545	T18079	T18076	arg2Of	expression,activated
R14546	T18079	T18077	arg1Of	expression,the
R14547	T18079	T18078	arg1Of	expression,MIP-1α
R14548	T18083	T18080	arg2Of	manner,in
R14549	T18083	T18081	arg1Of	manner,a
R14550	T18083	T18082	arg1Of	manner,p65-dependent
R14551	T18086	T18085	arg2Of	shown,as
R14552	T18086	T18092	arg1Of	shown,following
R14553	T18089	T18086	arg1Of	lack,shown
R14554	T18089	T18087	arg2Of	lack,by
R14555	T18089	T18088	arg1Of	lack,the
R14556	T18089	T18090	arg1Of	lack,of
R14557	T18091	T18090	arg2Of	effect,of
R14558	T18094	T18093	arg1Of	interference,RNA
R14559	T18094	T18095	arg1Of	interference,of
R14560	T18094	T18097	arg1Of	interference,or
R14561	T18096	T18095	arg2Of	Tat,of
R14562	T18097	T18092	arg2Of	or,following
R14563	T18098	T18097	arg2Of	p65,or
R14564	T18098	T18099	arg1Of	p65,(
R14565	T18101	T18099	arg2Of	6F,(
R14566	T18101	T18100	arg1Of	6F,Figure
R14567	T18102	T18099	arg3Of	),(
R14568	T18105	T18106	arg1Of	Tat,and
R14569	T18106	T18108	arg1Of	and,were
R14570	T18106	T18110	arg2Of	and,recruited
R14571	T18107	T18106	arg2Of	p65,and
R14572	T18110	T18103	arg1Of	recruited,Moreover
R14573	T18110	T18104	arg1Of	recruited,","
R14574	T18110	T18108	arg2Of	recruited,were
R14575	T18110	T18109	arg1Of	recruited,both
R14576	T18110	T18111	arg1Of	recruited,to
R14577	T18110	T18117	arg1Of	recruited,","
R14578	T18110	T18118	arg1Of	recruited,while
R14579	T18114	T18111	arg2Of	enhancer,to
R14580	T18114	T18112	arg1Of	enhancer,the
R14581	T18114	T18113	arg1Of	enhancer,NF-κB
R14582	T18114	T18115	arg1Of	enhancer,of
R14583	T18116	T18115	arg2Of	MIP-1α,of
R14584	T18121	T18119	arg1Of	occupancy,the
R14585	T18121	T18120	arg1Of	occupancy,p65
R14586	T18121	T18122	arg1Of	occupancy,was
R14587	T18121	T18123	arg2Of	occupancy,abolished
R14588	T18123	T18118	arg2Of	abolished,while
R14589	T18123	T18122	arg2Of	abolished,was
R14590	T18126	T18123	arg1Of	Tat,abolished
R14591	T18126	T18124	arg2Of	Tat,by
R14592	T18126	T18125	arg1Of	Tat,siRNA
R14593	T18126	T18127	arg1Of	Tat,(
R14594	T18129	T18128	arg1Of	6G,Figure
R14595	T18129	T18130	arg1Of	6G,and
R14596	T18130	T18127	arg2Of	and,(
R14597	T18131	T18130	arg2Of	H,and
R14598	T18132	T18127	arg3Of	),(
R14599	T18135	T18134	arg1Of	results,these
R14600	T18135	T18136	arg1Of	results,indicated
R14601	T18136	T18133	arg1Of	indicated,Altogether
R14602	T18142	T18138	arg2Of	infection,in
R14603	T18142	T18139	arg1Of	infection,single
R14604	T18142	T18140	arg1Of	infection,round
R14605	T18142	T18141	arg1Of	infection,HIV-1
R14606	T18143	T18144	arg1Of	Tat,associated
R14607	T18143	T18151	arg1Of	Tat,induced
R14608	T18144	T18145	arg1Of	associated,with
R14609	T18144	T18150	arg1Of	associated,and
R14610	T18146	T18147	arg1Of	IκB-α,and
R14611	T18147	T18145	arg2Of	and,with
R14612	T18148	T18147	arg2Of	p65,and
R14613	T18150	T18136	arg2Of	and,indicated
R14614	T18150	T18137	arg1Of	and,that
R14615	T18150	T18138	arg1Of	and,in
R14616	T18150	T18149	arg1Of	and,","
R14617	T18151	T18150	arg2Of	induced,and
R14618	T18151	T18158	arg1Of	induced,through
R14619	T18154	T18151	arg2Of	activation,induced
R14620	T18154	T18152	arg1Of	activation,the
R14621	T18154	T18153	arg1Of	activation,p65-dependent
R14622	T18154	T18155	arg1Of	activation,of
R14623	T18157	T18155	arg2Of	expression,of
R14624	T18157	T18156	arg1Of	expression,MIP-1α
R14625	T18159	T18160	arg1Of	occupancy,of
R14626	T18159	T18164	arg1Of	occupancy,and
R14627	T18163	T18160	arg2Of	promoter,of
R14628	T18163	T18161	arg1Of	promoter,the
R14629	T18163	T18162	arg1Of	promoter,MIP-1α
R14630	T18164	T18158	arg2Of	and,through
R14631	T18166	T18164	arg2Of	recruitment,and
R14632	T18166	T18165	arg2Of	recruitment,increased
R14633	T18166	T18167	arg1Of	recruitment,of
R14634	T18168	T18167	arg2Of	p65,of
R15863	T19912	T19911	arg1Of	study,This
R15864	T19912	T19913	arg1Of	study,reports
R15865	T19916	T19913	arg2Of	mechanism,reports
R15866	T19916	T19914	arg1Of	mechanism,a
R15867	T19916	T19915	arg1Of	mechanism,novel
R15868	T19916	T19917	arg1Of	mechanism,of
R15869	T19916	T19920	arg1Of	mechanism,by
R15870	T19919	T19917	arg2Of	activation,of
R15871	T19919	T19918	arg1Of	activation,NF-κB
R15872	T19924	T19920	arg2Of	transactivator,by
R15873	T19924	T19921	arg1Of	transactivator,the
R15874	T19924	T19922	arg1Of	transactivator,HIV-1
R15875	T19924	T19923	arg1Of	transactivator,Tat
R15876	T19926	T19925	arg2Of	on,Based
R15877	T19928	T19926	arg2Of	evidence,on
R15878	T19928	T19927	arg1Of	evidence,the
R15879	T19930	T19931	arg1Of	Tat,enhanced
R15880	T19930	T19947	arg1Of	Tat,interacted
R15881	T19931	T19945	arg1Of	enhanced,and
R15882	T19934	T19931	arg2Of	activity,enhanced
R15883	T19934	T19932	arg1Of	activity,the
R15884	T19934	T19933	arg1Of	activity,transcriptional
R15885	T19934	T19935	arg1Of	activity,of
R15886	T19938	T19935	arg2Of	subunit,of
R15887	T19938	T19936	arg1Of	subunit,the
R15888	T19938	T19937	arg1Of	subunit,p65
R15889	T19938	T19939	arg1Of	subunit,of
R15890	T19940	T19939	arg2Of	NF-κB,of
R15891	T19940	T19941	arg1Of	NF-κB,(
R15892	T19942	T19941	arg2Of	"49,63,64",(
R15893	T19943	T19941	arg3Of	),(
R15894	T19945	T19928	arg2Of	and,evidence
R15895	T19945	T19929	arg1Of	and,that
R15896	T19945	T19944	arg1Of	and,","
R15897	T19947	T19945	arg2Of	interacted,and
R15898	T19947	T19946	arg1Of	interacted,physically
R15899	T19947	T19948	arg1Of	interacted,with
R15900	T19951	T19948	arg2Of	repressor,with
R15901	T19951	T19949	arg1Of	repressor,the
R15902	T19951	T19950	arg1Of	repressor,IκB-α
R15903	T19951	T19952	arg1Of	repressor,(
R15904	T19953	T19952	arg2Of	"50,51",(
R15905	T19954	T19952	arg3Of	),(
R15906	T19956	T19957	arg1Of	we,investigated
R15907	T19957	T19925	arg1Of	investigated,Based
R15908	T19957	T19955	arg1Of	investigated,","
R15909	T19959	T19957	arg2Of	possibility,investigated
R15910	T19959	T19958	arg1Of	possibility,the
R15911	T19961	T19962	arg1Of	Tat,could
R15912	T19961	T19963	arg1Of	Tat,activate
R15913	T19963	T19959	arg2Of	activate,possibility
R15914	T19963	T19960	arg1Of	activate,that
R15915	T19963	T19962	arg2Of	activate,could
R15916	T19963	T19965	arg1Of	activate,via
R15917	T19964	T19963	arg2Of	NF-κB,activate
R15918	T19967	T19965	arg2Of	interaction,via
R15919	T19967	T19966	arg1Of	interaction,direct
R15920	T19967	T19968	arg1Of	interaction,with
R15921	T19969	T19970	arg1Of	IκB-α,and
R15922	T19970	T19968	arg2Of	and,with
R15923	T19971	T19970	arg2Of	p65,and
R15924	T19974	T19972	arg2Of	end,To
R15925	T19974	T19973	arg1Of	end,this
R15926	T19978	T19976	arg1Of	activity,the
R15927	T19978	T19977	arg1Of	activity,NF-κB
R15928	T19978	T19979	arg1Of	activity,was
R15929	T19978	T19980	arg2Of	activity,monitored
R15930	T19978	T19986	arg1Of	activity,using
R15931	T19978	T19990	arg1Of	activity,silence
R15932	T19980	T19972	arg1Of	monitored,To
R15933	T19980	T19975	arg1Of	monitored,","
R15934	T19980	T19979	arg2Of	monitored,was
R15935	T19980	T19981	arg1Of	monitored,in
R15936	T19985	T19981	arg2Of	infection,in
R15937	T19985	T19982	arg1Of	infection,single
R15938	T19985	T19983	arg1Of	infection,round
R15939	T19985	T19984	arg1Of	infection,HIV-1
R15940	T19986	T19980	arg3Of	using,monitored
R15941	T19988	T19986	arg2Of	interference,using
R15942	T19988	T19987	arg1Of	interference,RNA
R15943	T19990	T19986	arg3Of	silence,using
R15944	T19990	T19989	arg1Of	silence,to
R15945	T19993	T19990	arg2Of	expression,silence
R15946	T19993	T19991	arg1Of	expression,the
R15947	T19993	T19992	arg1Of	expression,Tat
R15948	T19996	T19994	arg2Of	approach,By
R15949	T19996	T19995	arg1Of	approach,this
R15950	T19998	T19999	arg1Of	we,avoided
R15951	T19999	T19994	arg1Of	avoided,By
R15952	T19999	T19997	arg1Of	avoided,","
R15953	T19999	T20007	arg1Of	avoided,to
R15954	T20001	T19999	arg2Of	perpetuation,avoided
R15955	T20001	T20000	arg1Of	perpetuation,the
R15956	T20001	T20002	arg1Of	perpetuation,of
R15957	T20005	T20002	arg2Of	signaling,of
R15958	T20005	T20003	arg1Of	signaling,NF-κB
R15959	T20005	T20004	arg1Of	signaling,activation
R15960	T20007	T20006	arg1Of	to,due
R15961	T20009	T20007	arg2Of	rounds,to
R15962	T20009	T20008	arg1Of	rounds,subsequent
R15963	T20009	T20010	arg1Of	rounds,of
R15964	T20012	T20010	arg2Of	entry,of
R15965	T20012	T20011	arg1Of	entry,viral
R15966	T20012	T20013	arg1Of	entry,in
R15967	T20016	T20013	arg2Of	propagation,in
R15968	T20016	T20014	arg1Of	propagation,cell
R15969	T20016	T20015	arg1Of	propagation,culture
R15970	T20016	T20017	arg1Of	propagation,(
R15971	T20018	T20017	arg2Of	"30,31",(
R15972	T20019	T20017	arg3Of	),(
R15973	T20022	T20020	arg2Of	infection,Upon
R15974	T20022	T20021	arg1Of	infection,HIV-1
R15975	T20027	T20024	arg1Of	activation,the
R15976	T20027	T20025	arg1Of	activation,early
R15977	T20027	T20026	arg1Of	activation,NF-κB
R15978	T20027	T20028	arg1Of	activation,occurred
R15979	T20028	T20020	arg1Of	occurred,Upon
R15980	T20028	T20023	arg1Of	occurred,","
R15981	T20028	T20029	arg1Of	occurred,concomitantly
R15982	T20028	T20030	arg1Of	occurred,with
R15983	T20028	T20036	arg1Of	occurred,in
R15984	T20028	T20042	arg1Of	occurred,after
R15985	T20032	T20031	arg1Of	activation,IKK
R15986	T20032	T20033	arg1Of	activation,and
R15987	T20033	T20030	arg2Of	and,with
R15988	T20035	T20033	arg2Of	degradation,and
R15989	T20035	T20034	arg1Of	degradation,IκB-α
R15990	T20038	T20036	arg2Of	absence,in
R15991	T20038	T20037	arg1Of	absence,the
R15992	T20038	T20039	arg1Of	absence,of
R15993	T20041	T20039	arg2Of	Soon,of
R15994	T20041	T20040	arg1Of	Soon,Tat.
R15995	T20044	T20043	arg1Of	shut,the
R15996	T20044	T20046	arg1Of	shut,of
R15997	T20044	T20052	arg1Of	shut,of
R15998	T20044	T20058	arg1Of	shut,was
R15999	T20044	T20059	arg2Of	shut,kept
R16000	T20044	T20060	arg1Of	shut,elevated
R16001	T20046	T20045	arg1Of	of,off
R16002	T20048	T20047	arg1Of	activity,IKK
R16003	T20048	T20049	arg1Of	activity,and
R16004	T20049	T20046	arg2Of	and,of
R16005	T20051	T20049	arg2Of	synthesis,and
R16006	T20051	T20050	arg1Of	synthesis,new
R16007	T20053	T20052	arg2Of	IκB-α,of
R16008	T20053	T20054	arg1Of	IκB-α,","
R16009	T20057	T20054	arg2Of	activity,","
R16010	T20057	T20055	arg1Of	activity,the
R16011	T20057	T20056	arg1Of	activity,NF-κB
R16012	T20059	T20042	arg2Of	kept,after
R16013	T20059	T20058	arg2Of	kept,was
R16014	T20059	T20061	arg1Of	kept,in
R16015	T20059	T20066	arg1Of	kept,","
R16016	T20059	T20067	arg1Of	kept,while
R16017	T20060	T20059	arg3Of	elevated,kept
R16018	T20063	T20061	arg2Of	presence,in
R16019	T20063	T20062	arg1Of	presence,the
R16020	T20063	T20064	arg1Of	presence,of
R16021	T20065	T20064	arg2Of	Tat,of
R16022	T20068	T20069	arg1Of	it,was
R16023	T20068	T20071	arg2Of	it,regulated
R16024	T20071	T20067	arg2Of	regulated,while
R16025	T20071	T20069	arg2Of	regulated,was
R16026	T20071	T20070	arg1Of	regulated,down
R16027	T20071	T20072	arg1Of	regulated,upon
R16028	T20073	T20072	arg2Of	silencing,upon
R16029	T20073	T20074	arg1Of	silencing,of
R16030	T20077	T20074	arg2Of	gene,of
R16031	T20077	T20075	arg1Of	gene,the
R16032	T20077	T20076	arg1Of	gene,Tat
R16033	T20079	T20078	arg1Of	findings,These
R16034	T20079	T20080	arg1Of	findings,indicate
R16035	T20086	T20082	arg2Of	infection,in
R16036	T20086	T20083	arg1Of	infection,single
R16037	T20086	T20084	arg1Of	infection,round
R16038	T20086	T20085	arg1Of	infection,HIV-1
R16039	T20088	T20089	arg1Of	Tat,enhanced
R16040	T20089	T20080	arg2Of	enhanced,indicate
R16041	T20089	T20081	arg1Of	enhanced,that
R16042	T20089	T20082	arg1Of	enhanced,in
R16043	T20089	T20087	arg1Of	enhanced,","
R16044	T20089	T20093	arg1Of	enhanced,without
R16045	T20092	T20089	arg2Of	activity,enhanced
R16046	T20092	T20090	arg1Of	activity,the
R16047	T20092	T20091	arg1Of	activity,NF-κB
R16048	T20094	T20093	arg2Of	affecting,without
R16049	T20097	T20095	arg1Of	activity,the
R16050	T20097	T20096	arg1Of	activity,IKK
R16051	T20097	T20098	arg1Of	activity,and
R16052	T20098	T20094	arg2Of	and,affecting
R16053	T20100	T20098	arg2Of	half-life,and
R16054	T20100	T20099	arg1Of	half-life,the
R16055	T20100	T20101	arg1Of	half-life,of
R16056	T20102	T20101	arg2Of	IκB-α,of
R16057	T20104	T20103	arg1Of	kinetic,Similar
R16058	T20104	T20105	arg1Of	kinetic,of
R16059	T20104	T20108	arg1Of	kinetic,occurred
R16060	T20107	T20105	arg2Of	activation,of
R16061	T20107	T20106	arg1Of	activation,NF-κB
R16062	T20108	T20109	arg1Of	occurred,upon
R16063	T20110	T20109	arg2Of	short-pulse,upon
R16064	T20110	T20111	arg1Of	short-pulse,of
R16065	T20110	T20113	arg1Of	short-pulse,in
R16066	T20112	T20111	arg2Of	PMA,of
R16067	T20116	T20113	arg2Of	cells,in
R16068	T20116	T20114	arg1Of	cells,Tat-transfected
R16069	T20116	T20115	arg1Of	cells,HeLa
R16070	T20116	T20117	arg1Of	cells,","
R16071	T20116	T20118	arg1Of	cells,where
R16072	T20119	T20120	arg1Of	Tat,inhibited
R16073	T20120	T20118	arg2Of	inhibited,where
R16074	T20120	T20124	arg1Of	inhibited,off
R16075	T20120	T20127	arg1Of	inhibited,in
R16076	T20123	T20120	arg2Of	turn,inhibited
R16077	T20123	T20121	arg1Of	turn,the
R16078	T20123	T20122	arg1Of	turn,post-activation
R16079	T20124	T20125	arg1Of	off,of
R16080	T20126	T20125	arg2Of	NF-κB,of
R16081	T20127	T20129	arg1Of	in,of
R16082	T20128	T20127	arg2Of	presence,in
R16083	T20132	T20127	arg3Of	IκB-α,in
R16084	T20132	T20130	arg1Of	IκB-α,newly
R16085	T20132	T20131	arg2Of	IκB-α,synthesized
R16086	T20135	T20133	arg1Of	activation,The
R16087	T20135	T20134	arg1Of	activation,Tat-dependent
R16088	T20135	T20136	arg1Of	activation,of
R16089	T20135	T20138	arg1Of	activation,correlated
R16090	T20137	T20136	arg2Of	NF-κB,of
R16091	T20138	T20139	arg1Of	correlated,with
R16092	T20141	T20139	arg2Of	association,with
R16093	T20141	T20140	arg1Of	association,the
R16094	T20141	T20142	arg1Of	association,of
R16095	T20141	T20146	arg1Of	association,with
R16096	T20145	T20142	arg2Of	protein,of
R16097	T20145	T20143	arg1Of	protein,the
R16098	T20145	T20144	arg1Of	protein,viral
R16099	T20147	T20146	arg2Of	IκB-α,with
R16100	T20147	T20148	arg1Of	IκB-α,","
R16101	T20147	T20149	arg1Of	IκB-α,which
R16102	T20147	T20150	arg1Of	IκB-α,interfered
R16103	T20150	T20151	arg1Of	interfered,with
R16104	T20153	T20151	arg2Of	generation,with
R16105	T20153	T20152	arg1Of	generation,the
R16106	T20153	T20154	arg1Of	generation,of
R16107	T20157	T20154	arg2Of	complex,of
R16108	T20157	T20155	arg1Of	complex,the
R16109	T20157	T20156	arg1Of	complex,IκB-α/p65
R16110	T20159	T20158	arg1Of	evidence,This
R16111	T20159	T20160	arg1Of	evidence,demonstrated
R16112	T20162	T20163	arg1Of	Tat,competed
R16113	T20162	T20172	arg1Of	Tat,prevented
R16114	T20163	T20164	arg1Of	competed,for
R16115	T20163	T20171	arg1Of	competed,and
R16116	T20166	T20164	arg2Of	binding,for
R16117	T20166	T20165	arg1Of	binding,the
R16118	T20166	T20167	arg1Of	binding,of
R16119	T20166	T20169	arg1Of	binding,to
R16120	T20168	T20167	arg2Of	IκB-α,of
R16121	T20170	T20169	arg2Of	p65,to
R16122	T20171	T20160	arg2Of	and,demonstrated
R16123	T20171	T20161	arg1Of	and,that
R16124	T20172	T20171	arg2Of	prevented,and
R16125	T20175	T20172	arg2Of	repression,prevented
R16126	T20175	T20173	arg1Of	repression,the
R16127	T20175	T20174	arg1Of	repression,IκB-α
R16128	T20175	T20176	arg1Of	repression,of
R16129	T20177	T20176	arg2Of	p65,of
R16130	T20180	T20178	arg2Of	mechanism,As
R16131	T20180	T20179	arg1Of	mechanism,additional
R16132	T20180	T20181	arg1Of	mechanism,of
R16133	T20183	T20181	arg2Of	activation,of
R16134	T20183	T20182	arg1Of	activation,NF-κB
R16135	T20185	T20186	arg1Of	Tat,associated
R16136	T20185	T20190	arg1Of	Tat,increased
R16137	T20186	T20187	arg1Of	associated,with
R16138	T20186	T20189	arg1Of	associated,and
R16139	T20188	T20187	arg2Of	p65,with
R16140	T20189	T20178	arg1Of	and,As
R16141	T20189	T20184	arg1Of	and,","
R16142	T20189	T20199	arg1Of	and,;
R16143	T20190	T20189	arg2Of	increased,and
R16144	T20194	T20190	arg2Of	affinity,increased
R16145	T20194	T20191	arg1Of	affinity,the
R16146	T20194	T20192	arg1Of	affinity,p65
R16147	T20194	T20193	arg1Of	affinity,binding
R16148	T20194	T20195	arg1Of	affinity,to
R16149	T20198	T20195	arg2Of	enhancer,to
R16150	T20198	T20196	arg1Of	enhancer,the
R16151	T20198	T20197	arg1Of	enhancer,NF-κB
R16152	T20201	T20200	arg1Of	evidence,this
R16153	T20201	T20202	arg1Of	evidence,was
R16154	T20201	T20203	arg2Of	evidence,obtained
R16155	T20203	T20199	arg2Of	obtained,;
R16156	T20203	T20202	arg2Of	obtained,was
R16157	T20203	T20204	arg1Of	obtained,with
R16158	T20206	T20204	arg2Of	proteins,with
R16159	T20206	T20205	arg1Of	proteins,recombinant
R16160	T20206	T20207	arg1Of	proteins,indicating
R16161	T20211	T20209	arg1Of	binding,the
R16162	T20211	T20210	arg1Of	binding,stronger
R16163	T20211	T20212	arg1Of	binding,of
R16164	T20211	T20214	arg1Of	binding,to
R16165	T20211	T20216	arg1Of	binding,was
R16166	T20213	T20212	arg2Of	p65,of
R16167	T20215	T20214	arg2Of	DNA,to
R16168	T20216	T20207	arg2Of	was,indicating
R16169	T20216	T20208	arg1Of	was,that
R16170	T20218	T20216	arg2Of	consequence,was
R16171	T20218	T20217	arg1Of	consequence,a
R16172	T20218	T20219	arg1Of	consequence,of
R16173	T20218	T20222	arg1Of	consequence,with
R16174	T20221	T20219	arg2Of	association,of
R16175	T20221	T20220	arg1Of	association,direct
R16176	T20223	T20222	arg2Of	Tat,with
R16177	T20223	T20224	arg1Of	Tat,","
R16178	T20223	T20225	arg1Of	Tat,which
R16179	T20223	T20227	arg1Of	Tat,caused
R16180	T20227	T20226	arg1Of	caused,likely
R16181	T20230	T20227	arg2Of	change,caused
R16182	T20230	T20228	arg1Of	change,a
R16183	T20230	T20229	arg1Of	change,conformational
R16184	T20230	T20231	arg1Of	change,of
R16185	T20232	T20231	arg2Of	p65,of
R16186	T20233	T20235	arg1Of	We,demonstrated
R16187	T20235	T20234	arg1Of	demonstrated,also
R16188	T20240	T20237	arg1Of	sequence,the
R16189	T20240	T20238	arg1Of	sequence,Tat
R16190	T20240	T20239	arg1Of	sequence,cysteine-rich
R16191	T20240	T20241	arg1Of	sequence,(
R16192	T20240	T20244	arg1Of	sequence,was
R16193	T20240	T20245	arg2Of	sequence,involved
R16194	T20242	T20241	arg2Of	"C22,25,27",(
R16195	T20243	T20241	arg3Of	),(
R16196	T20245	T20235	arg2Of	involved,demonstrated
R16197	T20245	T20236	arg1Of	involved,that
R16198	T20245	T20244	arg2Of	involved,was
R16199	T20245	T20246	arg1Of	involved,in
R16200	T20248	T20246	arg2Of	binding,in
R16201	T20248	T20247	arg1Of	binding,the
R16202	T20248	T20249	arg1Of	binding,to
R16203	T20251	T20249	arg2Of	RHD,to
R16204	T20251	T20250	arg1Of	RHD,the
R16205	T20251	T20252	arg1Of	RHD,of
R16206	T20253	T20254	arg1Of	p65,and
R16207	T20255	T20254	arg2Of	NF-κB,and
R16208	T20256	T20252	arg2Of	activation,of
R16209	T20256	T20253	arg1Of	activation,p65
R16210	T20256	T20255	arg1Of	activation,NF-κB
R16211	T20262	T20259	arg1Of	sequence,the
R16212	T20262	T20260	arg1Of	sequence,Tat
R16213	T20262	T20261	arg1Of	sequence,arginine-rich
R16214	T20262	T20263	arg1Of	sequence,(
R16215	T20262	T20266	arg1Of	sequence,was
R16216	T20262	T20267	arg2Of	sequence,required
R16217	T20264	T20263	arg2Of	"R49,52,53,55,56,57",(
R16218	T20265	T20263	arg3Of	),(
R16219	T20267	T20257	arg1Of	required,Differently
R16220	T20267	T20258	arg1Of	required,","
R16221	T20267	T20266	arg2Of	required,was
R16222	T20267	T20268	arg1Of	required,for
R16223	T20270	T20268	arg2Of	binding,for
R16224	T20270	T20269	arg1Of	binding,the
R16225	T20270	T20271	arg1Of	binding,to
R16226	T20270	T20282	arg1Of	binding,for
R16227	T20271	T20281	arg1Of	to,and
R16228	T20274	T20271	arg2Of	ankyrin,to
R16229	T20274	T20272	arg1Of	ankyrin,the
R16230	T20274	T20273	arg1Of	ankyrin,sixth
R16231	T20274	T20275	arg1Of	ankyrin,of
R16232	T20276	T20275	arg2Of	IκB-α,of
R16233	T20276	T20277	arg1Of	IκB-α,(
R16234	T20278	T20277	arg2Of	"50,51",(
R16235	T20279	T20277	arg3Of	),(
R16236	T20281	T20280	arg1Of	and,","
R16237	T20282	T20281	arg2Of	for,and
R16238	T20283	T20282	arg2Of	releasing,for
R16239	T20283	T20285	arg1Of	releasing,from
R16240	T20284	T20283	arg2Of	p65,releasing
R16241	T20288	T20285	arg2Of	inhibition,from
R16242	T20288	T20286	arg1Of	inhibition,the
R16243	T20288	T20287	arg1Of	inhibition,IκB-α
R16244	T20291	T20290	arg1Of	results,these
R16245	T20291	T20292	arg1Of	results,demonstrated
R16246	T20292	T20289	arg1Of	demonstrated,Altogether
R16247	T20294	T20295	arg1Of	Tat,abolished
R16248	T20294	T20303	arg1Of	Tat,hijacking
R16249	T20294	T20308	arg1Of	Tat,shielding
R16250	T20294	T20316	arg1Of	Tat,enforcing
R16251	T20295	T20292	arg2Of	abolished,demonstrated
R16252	T20295	T20293	arg1Of	abolished,that
R16253	T20295	T20302	arg1Of	abolished,by
R16254	T20299	T20295	arg2Of	regulation,abolished
R16255	T20299	T20296	arg1Of	regulation,the
R16256	T20299	T20297	arg1Of	regulation,negative
R16257	T20299	T20298	arg1Of	regulation,feedback
R16258	T20299	T20300	arg1Of	regulation,of
R16259	T20301	T20300	arg2Of	NF-κB,of
R16260	T20303	T20307	arg1Of	hijacking,","
R16261	T20303	T20308	modOf	hijacking,shielding
R16262	T20303	T20315	arg1Of	hijacking,and
R16263	T20306	T20303	arg2Of	repressor,hijacking
R16264	T20306	T20304	arg1Of	repressor,the
R16265	T20306	T20305	arg1Of	repressor,IκB-α
R16266	T20308	T20310	arg1Of	shielding,from
R16267	T20309	T20308	arg2Of	p65,shielding
R16268	T20313	T20310	arg2Of	embrace,from
R16269	T20313	T20311	arg1Of	embrace,the
R16270	T20313	T20312	arg1Of	embrace,IκB-α
R16271	T20315	T20302	arg2Of	and,by
R16272	T20315	T20314	arg1Of	and,","
R16273	T20316	T20315	arg2Of	enforcing,and
R16274	T20319	T20316	arg2Of	binding,enforcing
R16275	T20319	T20317	arg1Of	binding,the
R16276	T20319	T20318	arg1Of	binding,p65
R16277	T20319	T20320	arg1Of	binding,to
R16278	T20321	T20320	arg2Of	DNA,to
R16279	T20323	T20322	arg1Of	evidence,Further
R16280	T20323	T20324	arg1Of	evidence,of
R16281	T20323	T20329	arg1Of	evidence,showed
R16282	T20326	T20324	arg2Of	activation,of
R16283	T20326	T20325	arg1Of	activation,Tat
R16284	T20326	T20327	arg1Of	activation,of
R16285	T20328	T20327	arg2Of	NF-κB,of
R16286	T20332	T20331	arg1Of	Tat,wild-type
R16287	T20332	T20334	arg1Of	Tat,and
R16288	T20334	T20329	arg2Of	and,showed
R16289	T20334	T20330	arg1Of	and,that
R16290	T20334	T20333	arg1Of	and,","
R16291	T20334	T20335	arg1Of	and,not
R16292	T20338	T20334	arg2Of	mutants,and
R16293	T20338	T20336	arg1Of	mutants,the
R16294	T20338	T20337	arg1Of	mutants,Tat
R16295	T20338	T20339	arg1Of	mutants,lacking
R16296	T20341	T20340	arg1Of	arginine-,the
R16297	T20341	T20342	arg1Of	arginine-,or
R16298	T20343	T20342	arg2Of	cysteine-rich,or
R16299	T20344	T20341	arg1Of	domains,arginine-
R16300	T20344	T20343	arg1Of	domains,cysteine-rich
R16301	T20344	T20345	arg1Of	domains,","
R16302	T20344	T20346	arg2Of	domains,up-regulated
R16303	T20346	T20348	arg1Of	up-regulated,vivo
R16304	T20348	T20347	arg1Of	vivo,in
R16305	T20350	T20339	arg2Of	expression,lacking
R16306	T20350	T20344	arg1Of	expression,domains
R16307	T20350	T20349	arg1Of	expression,the
R16308	T20350	T20351	arg1Of	expression,of
R16309	T20353	T20351	arg2Of	number,of
R16310	T20353	T20352	arg1Of	number,a
R16311	T20353	T20354	arg1Of	number,of
R16312	T20356	T20354	arg2Of	genes,of
R16313	T20356	T20355	arg1Of	genes,NF-κB-responsive
R16314	T20356	T20357	arg1Of	genes,","
R16315	T20356	T20358	arg1Of	genes,including
R16316	T20359	T20360	arg1Of	MIP-1α,","
R16317	T20360	T20362	arg1Of	",",","
R16318	T20361	T20360	arg2Of	CSF3,","
R16319	T20362	T20364	arg1Of	",",","
R16320	T20363	T20362	arg2Of	LTA,","
R16321	T20364	T20366	arg1Of	",",and
R16322	T20365	T20364	arg2Of	NFKBIA,","
R16323	T20366	T20358	arg2Of	and,including
R16324	T20367	T20366	arg2Of	TLR2,and
R16325	T20370	T20368	arg2Of	analysis,By
R16326	T20370	T20369	arg1Of	analysis,ChIP
R16327	T20372	T20373	arg1Of	we,observed
R16328	T20373	T20368	arg1Of	observed,By
R16329	T20373	T20371	arg1Of	observed,","
R16330	T20375	T20376	arg1Of	Tat,increased
R16331	T20375	T20391	arg1Of	Tat,was
R16332	T20375	T20392	arg2Of	Tat,recovered
R16333	T20376	T20390	arg1Of	increased,and
R16334	T20378	T20376	arg2Of	recruitment,increased
R16335	T20378	T20377	arg1Of	recruitment,the
R16336	T20378	T20379	arg1Of	recruitment,of
R16337	T20378	T20381	arg1Of	recruitment,to
R16338	T20380	T20379	arg2Of	p65,of
R16339	T20384	T20381	arg2Of	enhancers,to
R16340	T20384	T20382	arg1Of	enhancers,the
R16341	T20384	T20383	arg1Of	enhancers,NF-κB
R16342	T20384	T20385	arg1Of	enhancers,of
R16343	T20388	T20385	arg2Of	genes,of
R16344	T20388	T20386	arg1Of	genes,the
R16345	T20388	T20387	arg2Of	genes,activated
R16346	T20390	T20373	arg2Of	and,observed
R16347	T20390	T20374	arg1Of	and,that
R16348	T20390	T20389	arg1Of	and,","
R16349	T20392	T20390	arg2Of	recovered,and
R16350	T20392	T20391	arg2Of	recovered,was
R16351	T20392	T20393	arg1Of	recovered,at
R16352	T20396	T20393	arg2Of	sites,at
R16353	T20396	T20394	arg1Of	sites,the
R16354	T20396	T20395	arg1Of	sites,same
R16355	T20398	T20397	arg1Of	interference,RNA
R16356	T20398	T20399	arg1Of	interference,of
R16357	T20398	T20401	arg1Of	interference,abolished
R16358	T20400	T20399	arg2Of	p65,of
R16359	T20404	T20401	arg2Of	occupancy,abolished
R16360	T20404	T20402	arg1Of	occupancy,the
R16361	T20404	T20403	arg1Of	occupancy,Tat
R16362	T20404	T20405	arg1Of	occupancy,of
R16363	T20408	T20405	arg2Of	enhancers,of
R16364	T20408	T20406	arg1Of	enhancers,the
R16365	T20408	T20407	arg1Of	enhancers,NF-κB
R16366	T20408	T20409	arg1Of	enhancers,indicating
R16367	T20413	T20411	arg1Of	binding,the
R16368	T20413	T20412	arg1Of	binding,Tat
R16369	T20413	T20414	arg1Of	binding,to
R16370	T20413	T20418	arg1Of	binding,was
R16371	T20413	T20419	arg2Of	binding,mediated
R16372	T20417	T20414	arg2Of	sites,to
R16373	T20417	T20415	arg1Of	sites,the
R16374	T20417	T20416	arg1Of	sites,NF-κB
R16375	T20419	T20409	arg2Of	mediated,indicating
R16376	T20419	T20410	arg1Of	mediated,that
R16377	T20419	T20418	arg2Of	mediated,was
R16378	T20421	T20419	arg1Of	p65,mediated
R16379	T20421	T20420	arg2Of	p65,by
R16380	T20423	T20422	arg1Of	results,These
R16381	T20423	T20424	arg1Of	results,were
R16382	T20423	T20425	arg1Of	results,consistent
R16383	T20425	T20424	arg2Of	consistent,were
R16384	T20425	T20426	arg1Of	consistent,with
R16385	T20428	T20426	arg2Of	evidence,with
R16386	T20428	T20427	arg1Of	evidence,the
R16387	T20430	T20431	arg1Of	Tat,displaced
R16388	T20430	T20446	arg1Of	Tat,increased
R16389	T20431	T20433	arg1Of	displaced,from
R16390	T20431	T20445	arg1Of	displaced,and
R16391	T20432	T20431	arg2Of	p65,displaced
R16392	T20435	T20433	arg2Of	binding,from
R16393	T20435	T20434	arg1Of	binding,the
R16394	T20435	T20436	arg1Of	binding,to
R16395	T20437	T20436	arg2Of	IκB-α,to
R16396	T20437	T20438	arg1Of	IκB-α,(
R16397	T20440	T20441	arg1Of	2D,and
R16398	T20441	T20438	arg2Of	and,(
R16399	T20441	T20439	arg1Of	and,Figures
R16400	T20442	T20441	arg2Of	3C,and
R16401	T20443	T20438	arg3Of	),(
R16402	T20445	T20428	arg2Of	and,evidence
R16403	T20445	T20429	arg1Of	and,that
R16404	T20445	T20444	arg1Of	and,","
R16405	T20446	T20445	arg2Of	increased,and
R16406	T20446	T20452	arg1Of	increased,through
R16407	T20451	T20446	arg2Of	affinity,increased
R16408	T20451	T20447	arg1Of	affinity,the
R16409	T20451	T20448	arg1Of	affinity,p65
R16410	T20451	T20449	arg1Of	affinity,DNA
R16411	T20451	T20450	arg1Of	affinity,binding
R16412	T20453	T20452	arg2Of	association,through
R16413	T20453	T20454	arg1Of	association,(
R16414	T20456	T20455	arg1Of	4E,Figure
R16415	T20456	T20457	arg1Of	4E,and
R16416	T20457	T20454	arg2Of	and,(
R16417	T20458	T20457	arg2Of	F,and
R16418	T20459	T20454	arg3Of	),(
R16419	T20462	T20464	arg1Of	Tat,increased
R16420	T20462	T20474	arg1Of	Tat,associating
R16421	T20462	T20479	arg1Of	Tat,interfering
R16422	T20464	T20460	arg1Of	increased,Thus
R16423	T20464	T20461	arg1Of	increased,","
R16424	T20464	T20463	arg1Of	increased,likely
R16425	T20464	T20473	arg1Of	increased,by
R16426	T20464	T20478	arg1Of	increased,by
R16427	T20466	T20464	arg2Of	recruitment,increased
R16428	T20466	T20465	arg1Of	recruitment,the
R16429	T20466	T20467	arg1Of	recruitment,of
R16430	T20466	T20469	arg1Of	recruitment,to
R16431	T20468	T20467	arg2Of	p65,of
R16432	T20472	T20469	arg2Of	promoters,to
R16433	T20472	T20470	arg1Of	promoters,the
R16434	T20472	T20471	arg1Of	promoters,NF-κB-dependent
R16435	T20473	T20477	arg1Of	by,and
R16436	T20474	T20473	arg2Of	associating,by
R16437	T20474	T20475	arg1Of	associating,with
R16438	T20476	T20475	arg2Of	p65,with
R16439	T20478	T20477	arg2Of	by,and
R16440	T20479	T20478	arg2Of	interfering,by
R16441	T20479	T20480	arg1Of	interfering,with
R16442	T20482	T20480	arg2Of	assembly,with
R16443	T20482	T20481	arg1Of	assembly,the
R16444	T20482	T20483	arg1Of	assembly,of
R16445	T20484	T20483	arg2Of	p65,of
R16446	T20484	T20485	arg1Of	p65,with
R16447	T20488	T20485	arg2Of	IκB-α,with
R16448	T20488	T20486	arg1Of	IκB-α,the
R16449	T20488	T20487	arg1Of	IκB-α,repressor
R16450	T20490	T20489	arg2Of	interest,Of
R16451	T20492	T20493	arg1Of	IκB-α,was
R16452	T20492	T20494	arg2Of	IκB-α,found
R16453	T20492	T20495	arg2Of	IκB-α,associated
R16454	T20494	T20489	arg1Of	found,Of
R16455	T20494	T20491	arg1Of	found,","
R16456	T20494	T20493	arg2Of	found,was
R16457	T20495	T20494	arg3Of	associated,found
R16458	T20495	T20496	arg1Of	associated,to
R16459	T20495	T20500	arg1Of	associated,in
R16460	T20499	T20496	arg2Of	enhancers,to
R16461	T20499	T20497	arg1Of	enhancers,the
R16462	T20499	T20498	arg1Of	enhancers,NF-κB
R16463	T20500	T20502	arg1Of	in,of
R16464	T20501	T20500	arg2Of	absence,in
R16465	T20503	T20500	arg3Of	Tat,in
R16466	T20503	T20504	arg1Of	Tat,","
R16467	T20503	T20505	arg1Of	Tat,where
R16468	T20506	T20507	arg1Of	it,was
R16469	T20506	T20508	arg2Of	it,displaced
R16470	T20508	T20505	arg2Of	displaced,where
R16471	T20508	T20507	arg2Of	displaced,was
R16472	T20508	T20509	arg1Of	displaced,in
R16473	T20510	T20509	arg2Of	presence,in
R16474	T20510	T20511	arg1Of	presence,of
R16475	T20512	T20511	arg2Of	Tat.,of
R16476	T20514	T20513	arg1Of	results,These
R16477	T20514	T20515	arg1Of	results,suggest
R16478	T20515	T20508	arg3Of	suggest,displaced
R16479	T20519	T20515	arg2Of	role,suggest
R16480	T20519	T20516	arg1Of	role,a
R16481	T20519	T20517	arg1Of	role,negative
R16482	T20519	T20518	arg1Of	role,regulatory
R16483	T20519	T20520	arg1Of	role,of
R16484	T20519	T20522	arg1Of	role,in
R16485	T20521	T20520	arg2Of	IκB-α,of
R16486	T20524	T20522	arg2Of	regulation,in
R16487	T20524	T20523	arg1Of	regulation,gene
R16488	T20524	T20525	arg1Of	regulation,through
R16489	T20526	T20525	arg2Of	occupancy,through
R16490	T20526	T20527	arg1Of	occupancy,of
R16491	T20529	T20527	arg2Of	promoters,of
R16492	T20529	T20528	arg1Of	promoters,specific
R16493	T20532	T20533	arg1Of	IκB-α,was
R16494	T20532	T20534	arg2Of	IκB-α,found
R16495	T20532	T20535	arg2Of	IκB-α,associated
R16496	T20534	T20530	arg1Of	found,Indeed
R16497	T20534	T20531	arg1Of	found,","
R16498	T20534	T20533	arg2Of	found,was
R16499	T20534	T20543	arg1Of	found,","
R16500	T20534	T20544	arg1Of	found,from
R16501	T20535	T20534	arg3Of	associated,found
R16502	T20535	T20536	arg1Of	associated,with
R16503	T20535	T20539	arg1Of	associated,at
R16504	T20538	T20536	arg2Of	deacetylases,with
R16505	T20538	T20537	arg1Of	deacetylases,hystone
R16506	T20542	T20539	arg2Of	promoter,at
R16507	T20542	T20540	arg1Of	promoter,the
R16508	T20542	T20541	arg1Of	promoter,hes1
R16509	T20545	T20544	arg2Of	where,from
R16510	T20546	T20547	arg1Of	it,was
R16511	T20546	T20548	arg2Of	it,removed
R16512	T20548	T20545	arg1Of	removed,where
R16513	T20548	T20547	arg2Of	removed,was
R16514	T20548	T20549	arg1Of	removed,following
R16515	T20551	T20549	arg2Of	stimulation,following
R16516	T20551	T20550	arg1Of	stimulation,TNF-α
R16517	T20551	T20552	arg1Of	stimulation,causing
R16518	T20554	T20553	arg1Of	acetylation,histone
R16519	T20554	T20555	arg1Of	acetylation,and
R16520	T20555	T20552	arg2Of	and,causing
R16521	T20558	T20555	arg2Of	activation,and
R16522	T20558	T20556	arg1Of	activation,hes1
R16523	T20558	T20557	arg1Of	activation,transcriptional
R16524	T20558	T20559	arg1Of	activation,(
R16525	T20560	T20559	arg2Of	65,(
R16526	T20561	T20559	arg3Of	),(
R16527	T20564	T20562	arg1Of	mechanism,A
R16528	T20564	T20563	arg1Of	mechanism,similar
R16529	T20564	T20565	arg1Of	mechanism,of
R16530	T20564	T20568	arg1Of	mechanism,could
R16531	T20564	T20569	arg1Of	mechanism,apply
R16532	T20567	T20565	arg2Of	regulation,of
R16533	T20567	T20566	arg1Of	regulation,gene
R16534	T20569	T20568	arg2Of	apply,could
R16535	T20569	T20570	arg1Of	apply,to
R16536	T20573	T20570	arg2Of	genes,to
R16537	T20573	T20571	arg1Of	genes,the
R16538	T20573	T20572	arg1Of	genes,NF-κB-dependent
R16539	T20573	T20574	arg2Of	genes,analysed
R16540	T20573	T20578	arg1Of	genes,","
R16541	T20573	T20579	arg1Of	genes,where
R16542	T20574	T20575	arg1Of	analysed,in
R16543	T20577	T20575	arg2Of	study,in
R16544	T20577	T20576	arg1Of	study,this
R16545	T20580	T20581	arg1Of	Tat,could
R16546	T20580	T20582	arg1Of	Tat,activate
R16547	T20580	T20587	arg1Of	Tat,removing
R16548	T20580	T20590	arg1Of	Tat,promoting
R16549	T20582	T20579	arg2Of	activate,where
R16550	T20582	T20581	arg2Of	activate,could
R16551	T20582	T20586	arg1Of	activate,by
R16552	T20585	T20582	arg2Of	expression,activate
R16553	T20585	T20583	arg1Of	expression,the
R16554	T20585	T20584	arg1Of	expression,gene
R16555	T20587	T20589	arg1Of	removing,and
R16556	T20588	T20587	arg2Of	IκB-α,removing
R16557	T20589	T20586	arg2Of	and,by
R16558	T20590	T20589	arg2Of	promoting,and
R16559	T20593	T20590	arg2Of	loading,promoting
R16560	T20593	T20591	arg1Of	loading,the
R16561	T20593	T20592	arg1Of	loading,p65
R16562	T20593	T20594	arg1Of	loading,(
R16563	T20595	T20594	arg2Of	Figure,(
R16564	T20595	T20596	arg1Of	Figure,7
R16565	T20597	T20594	arg3Of	),(
R16566	T20600	T20598	arg1Of	relevance,The
R16567	T20600	T20599	arg1Of	relevance,physiological
R16568	T20600	T20601	arg1Of	relevance,of
R16569	T20600	T20607	arg1Of	relevance,was
R16570	T20600	T20608	arg2Of	relevance,demonstrated
R16571	T20604	T20601	arg2Of	talk,of
R16572	T20604	T20602	arg1Of	talk,Tat
R16573	T20604	T20603	arg1Of	talk,cross
R16574	T20604	T20605	arg1Of	talk,with
R16575	T20606	T20605	arg2Of	NF-κB,with
R16576	T20608	T20607	arg2Of	demonstrated,was
R16577	T20608	T20609	arg1Of	demonstrated,in
R16578	T20612	T20609	arg2Of	infection,in
R16579	T20612	T20610	arg1Of	infection,the
R16580	T20612	T20611	arg1Of	infection,HIV-1
R16581	T20612	T20613	arg1Of	infection,of
R16582	T20612	T20616	arg1Of	infection,","
R16583	T20612	T20617	arg1Of	infection,where
R16584	T20615	T20613	arg2Of	monocytes,of
R16585	T20615	T20614	arg1Of	monocytes,human
R16586	T20620	T20618	arg1Of	protein,HIV-1-encoded
R16587	T20620	T20619	arg1Of	protein,Tat
R16588	T20620	T20621	arg2Of	protein,counteracted
R16589	T20620	T20635	arg1Of	protein,sustained
R16590	T20621	T20624	arg1Of	counteracted,off
R16591	T20621	T20627	arg1Of	counteracted,","
R16592	T20621	T20628	arg1Of	counteracted,as
R16593	T20623	T20621	arg3Of	turn,counteracted
R16594	T20623	T20622	arg1Of	turn,post-activation
R16595	T20624	T20625	arg1Of	off,of
R16596	T20626	T20625	arg2Of	NF-κB,of
R16597	T20629	T20628	arg2Of	shown,as
R16598	T20629	T20630	arg1Of	shown,by
R16599	T20630	T20631	arg1Of	by,:
R16600	T20633	T20632	arg1Of	i,(
R16601	T20633	T20634	arg1Of	i,)
R16602	T20635	T20617	arg2Of	sustained,where
R16603	T20635	T20633	arg1Of	sustained,i
R16604	T20637	T20636	arg1Of	activity,NF-κB
R16605	T20637	T20638	arg1Of	activity,by
R16606	T20637	T20640	arg1Of	activity,in
R16607	T20637	T20646	arg1Of	activity,;
R16608	T20639	T20638	arg2Of	Tat,by
R16609	T20641	T20640	arg2Of	presence,in
R16610	T20641	T20642	arg1Of	presence,of
R16611	T20644	T20643	arg1Of	synthesized,newly
R16612	T20645	T20642	arg2Of	IκB-α,of
R16613	T20645	T20644	arg1Of	IκB-α,synthesized
R16614	T20646	T20635	arg2Of	;,sustained
R16615	T20648	T20647	arg1Of	ii,(
R16616	T20648	T20649	arg1Of	ii,)
R16617	T20650	T20648	arg1Of	coimmunoprecipitation,ii
R16618	T20650	T20651	arg1Of	coimmunoprecipitation,of
R16619	T20650	T20653	arg1Of	coimmunoprecipitation,with
R16620	T20650	T20657	arg1Of	coimmunoprecipitation,;
R16621	T20652	T20651	arg2Of	Tat,of
R16622	T20654	T20655	arg1Of	IκB-α,and
R16623	T20655	T20653	arg2Of	and,with
R16624	T20656	T20655	arg2Of	p65,and
R16625	T20657	T20665	arg1Of	;,dependent
R16626	T20657	T20670	arg1Of	;,;
R16627	T20659	T20660	arg1Of	iii,)
R16628	T20661	T20657	arg2Of	induction,;
R16629	T20661	T20658	arg1Of	induction,(
R16630	T20661	T20659	arg1Of	induction,iii
R16631	T20661	T20662	arg1Of	induction,of
R16632	T20664	T20662	arg2Of	expression,of
R16633	T20664	T20663	arg1Of	expression,MIP-1α
R16634	T20665	T20666	arg1Of	dependent,on
R16635	T20667	T20668	arg1Of	Tat,and
R16636	T20668	T20666	arg2Of	and,on
R16637	T20669	T20668	arg2Of	p65,and
R16638	T20670	T20646	arg2Of	;,;
R16639	T20672	T20673	arg1Of	iv,)
R16640	T20675	T20670	arg2Of	occupancy,;
R16641	T20675	T20671	arg1Of	occupancy,(
R16642	T20675	T20672	arg1Of	occupancy,iv
R16643	T20675	T20674	arg1Of	occupancy,Tat
R16644	T20675	T20676	arg1Of	occupancy,of
R16645	T20680	T20676	arg2Of	enhancer,of
R16646	T20680	T20677	arg1Of	enhancer,the
R16647	T20680	T20678	arg1Of	enhancer,MIP-1α
R16648	T20680	T20679	arg1Of	enhancer,NF-κB
R16649	T20680	T20681	arg2Of	enhancer,associated
R16650	T20681	T20682	arg1Of	associated,with
R16651	T20684	T20682	arg2Of	recruitment,with
R16652	T20684	T20683	arg2Of	recruitment,increased
R16653	T20684	T20685	arg1Of	recruitment,of
R16654	T20686	T20685	arg2Of	p65,of
R16655	T20688	T20687	arg1Of	study,This
R16656	T20688	T20689	arg1Of	study,supports
R16657	T20689	T20695	arg1Of	supports,through
R16658	T20692	T20689	arg2Of	action,supports
R16659	T20692	T20690	arg1Of	action,the
R16660	T20692	T20691	arg1Of	action,pro-inflammatory
R16661	T20692	T20693	arg1Of	action,of
R16662	T20694	T20693	arg2Of	Tat,of
R16663	T20697	T20695	arg2Of	interaction,through
R16664	T20697	T20696	arg1Of	interaction,physical
R16665	T20697	T20698	arg1Of	interaction,with
R16666	T20699	T20700	arg1Of	p65,and
R16667	T20700	T20698	arg2Of	and,with
R16668	T20701	T20700	arg2Of	IκB-α,and
R16669	T20704	T20702	arg1Of	cytokines,Several
R16670	T20704	T20703	arg1Of	cytokines,inflammatory
R16671	T20704	T20705	arg1Of	cytokines,under
R16672	T20704	T20711	arg1Of	cytokines,are
R16673	T20704	T20712	arg1Of	cytokines,hyper-expressed
R16674	T20708	T20705	arg2Of	control,under
R16675	T20708	T20706	arg1Of	control,the
R16676	T20708	T20707	arg1Of	control,transcriptional
R16677	T20708	T20709	arg1Of	control,of
R16678	T20710	T20709	arg2Of	NF-κB,of
R16679	T20712	T20711	arg2Of	hyper-expressed,are
R16680	T20712	T20713	arg1Of	hyper-expressed,in
R16681	T20716	T20713	arg2Of	individuals,in
R16682	T20716	T20714	arg1Of	individuals,HIV-1
R16683	T20716	T20715	arg1Of	individuals,infected
R16684	T20716	T20717	arg1Of	individuals,","
R16685	T20716	T20718	arg1Of	individuals,including
R16686	T20719	T20720	arg1Of	IL-6,(
R16687	T20719	T20723	arg1Of	IL-6,","
R16688	T20721	T20720	arg2Of	"66,67",(
R16689	T20722	T20720	arg3Of	),(
R16690	T20723	T20729	arg1Of	",",and
R16691	T20724	T20723	arg2Of	TNF-α,","
R16692	T20724	T20725	arg1Of	TNF-α,(
R16693	T20726	T20725	arg2Of	68,(
R16694	T20727	T20725	arg3Of	),(
R16695	T20729	T20718	arg2Of	and,including
R16696	T20729	T20728	arg1Of	and,","
R16697	T20730	T20729	arg2Of	MIP-1α,and
R16698	T20730	T20731	arg1Of	MIP-1α,(
R16699	T20732	T20731	arg2Of	"57–62,69–71",(
R16700	T20733	T20731	arg3Of	),(
R16701	T20736	T20734	arg2Of	case,In
R16702	T20736	T20735	arg1Of	case,the
R16703	T20736	T20737	arg1Of	case,of
R16704	T20738	T20739	arg1Of	TNF-α,and
R16705	T20739	T20737	arg2Of	and,of
R16706	T20740	T20739	arg2Of	IL-6,and
R16707	T20742	T20743	arg1Of	Tat,activated
R16708	T20742	T20748	arg1Of	Tat,binding
R16709	T20742	T20761	arg1Of	Tat,promoting
R16710	T20743	T20734	arg1Of	activated,In
R16711	T20743	T20741	arg1Of	activated,","
R16712	T20743	T20747	arg1Of	activated,by
R16713	T20746	T20743	arg2Of	expression,activated
R16714	T20746	T20744	arg1Of	expression,the
R16715	T20746	T20745	arg1Of	expression,cytokine
R16716	T20748	T20749	arg1Of	binding,to
R16717	T20748	T20758	arg1Of	binding,","
R16718	T20753	T20749	arg2Of	loop,to
R16719	T20753	T20750	arg1Of	loop,the
R16720	T20753	T20751	arg1Of	loop,TAR-like
R16721	T20753	T20752	arg1Of	loop,stem
R16722	T20753	T20754	arg1Of	loop,of
R16723	T20757	T20754	arg2Of	transcript,of
R16724	T20757	T20755	arg1Of	transcript,the
R16725	T20757	T20756	arg1Of	transcript,5′
R16726	T20758	T20747	arg2Of	",",by
R16727	T20761	T20758	arg2Of	promoting,","
R16728	T20761	T20759	arg1Of	promoting,thus
R16729	T20761	T20760	arg1Of	promoting,likely
R16730	T20764	T20761	arg2Of	elongation,promoting
R16731	T20764	T20762	arg1Of	elongation,the
R16732	T20764	T20763	arg1Of	elongation,transcriptional
R16733	T20764	T20765	arg1Of	elongation,(
R16734	T20766	T20765	arg2Of	"33,37,47",(
R16735	T20767	T20765	arg3Of	),(
R16736	T20770	T20771	arg1Of	we,have
R16737	T20770	T20772	arg1Of	we,shown
R16738	T20772	T20768	arg1Of	shown,Here
R16739	T20772	T20769	arg1Of	shown,","
R16740	T20772	T20771	arg2Of	shown,have
R16741	T20777	T20774	arg1Of	activation,the
R16742	T20777	T20775	arg1Of	activation,Tat-dependent
R16743	T20777	T20776	arg1Of	activation,transcriptional
R16744	T20777	T20778	arg1Of	activation,of
R16745	T20777	T20784	arg1Of	activation,required
R16746	T20780	T20778	arg2Of	number,of
R16747	T20780	T20779	arg1Of	number,a
R16748	T20780	T20781	arg1Of	number,of
R16749	T20783	T20781	arg2Of	genes,of
R16750	T20783	T20782	arg1Of	genes,pro-inflammatory
R16751	T20784	T20772	arg2Of	required,shown
R16752	T20784	T20773	arg1Of	required,that
R16753	T20784	T20795	arg1Of	required,together
R16754	T20786	T20784	arg2Of	assembly,required
R16755	T20786	T20785	arg1Of	assembly,the
R16756	T20786	T20787	arg1Of	assembly,of
R16757	T20786	T20789	arg1Of	assembly,with
R16758	T20786	T20791	arg1Of	assembly,at
R16759	T20788	T20787	arg2Of	Tat,of
R16760	T20790	T20789	arg2Of	p65,with
R16761	T20794	T20791	arg2Of	enhancer,at
R16762	T20794	T20792	arg1Of	enhancer,the
R16763	T20794	T20793	arg1Of	enhancer,NF-κB
R16764	T20795	T20796	arg1Of	together,with
R16765	T20798	T20796	arg2Of	displacement,with
R16766	T20798	T20797	arg1Of	displacement,the
R16767	T20798	T20799	arg1Of	displacement,of
R16768	T20800	T20799	arg2Of	IκB-α,of
R16769	T20800	T20801	arg1Of	IκB-α,(
R16770	T20802	T20801	arg2Of	Figure,(
R16771	T20802	T20803	arg1Of	Figure,5
R16772	T20804	T20801	arg3Of	),(
R16773	T20809	T20807	arg2Of	set,in
R16774	T20809	T20808	arg1Of	set,the
R16775	T20809	T20810	arg1Of	set,of
R16776	T20813	T20810	arg2Of	genes,of
R16777	T20813	T20811	arg1Of	genes,distinct
R16778	T20813	T20812	arg1Of	genes,NF-κB-responsive
R16779	T20815	T20816	arg1Of	Tat,acted
R16780	T20816	T20805	arg1Of	acted,Thus
R16781	T20816	T20806	arg1Of	acted,","
R16782	T20816	T20807	arg1Of	acted,in
R16783	T20816	T20814	arg1Of	acted,","
R16784	T20816	T20817	arg1Of	acted,as
R16785	T20819	T20817	arg2Of	component,as
R16786	T20819	T20818	arg1Of	component,a
R16787	T20819	T20820	arg1Of	component,of
R16788	T20824	T20820	arg2Of	complex,of
R16789	T20824	T20821	arg1Of	complex,the
R16790	T20824	T20822	arg1Of	complex,transcriptional
R16791	T20824	T20823	arg1Of	complex,initiation
R16792	T20824	T20825	arg1Of	complex,","
R16793	T20824	T20826	arg1Of	complex,which
R16794	T20824	T20827	arg1Of	complex,is
R16795	T20824	T20828	arg1Of	complex,consistent
R16796	T20828	T20827	arg2Of	consistent,is
R16797	T20828	T20829	arg1Of	consistent,with
R16798	T20831	T20829	arg2Of	reports,with
R16799	T20831	T20830	arg1Of	reports,previous
R16800	T20831	T20832	arg1Of	reports,on
R16801	T20831	T20834	arg1Of	reports,promoting
R16802	T20833	T20832	arg2Of	Tat,on
R16803	T20834	T20837	arg1Of	promoting,via
R16804	T20836	T20834	arg2Of	transcription,promoting
R16805	T20836	T20835	arg1Of	transcription,the
R16806	T20839	T20837	arg2Of	motifs,via
R16807	T20839	T20838	arg1Of	motifs,DNA
R16808	T20839	T20840	arg1Of	motifs,(
R16809	T20841	T20840	arg2Of	"72,73",(
R16810	T20842	T20840	arg3Of	),(
R16811	T20843	T20844	arg1Of	Overproduction,of
R16812	T20843	T20847	arg1Of	Overproduction,is
R16813	T20843	T20848	arg2Of	Overproduction,considered
R16814	T20846	T20844	arg2Of	cytokines,of
R16815	T20846	T20845	arg1Of	cytokines,pro-inflammatory
R16816	T20848	T20847	arg2Of	considered,is
R16817	T20851	T20848	arg3Of	mechanism,considered
R16818	T20851	T20849	arg1Of	mechanism,a
R16819	T20851	T20850	arg1Of	mechanism,major
R16820	T20851	T20852	arg1Of	mechanism,of
R16821	T20854	T20853	arg1Of	deregulation,immune
R16822	T20854	T20855	arg1Of	deregulation,(
R16823	T20854	T20858	arg1Of	deregulation,and
R16824	T20856	T20855	arg2Of	26,(
R16825	T20857	T20855	arg3Of	),(
R16826	T20858	T20852	arg2Of	and,of
R16827	T20860	T20858	arg2Of	dysfunction,and
R16828	T20860	T20859	arg1Of	dysfunction,neuron
R16829	T20860	T20861	arg1Of	dysfunction,in
R16830	T20862	T20861	arg2Of	AIDS,in
R16831	T20862	T20863	arg1Of	AIDS,(
R16832	T20864	T20863	arg2Of	"74,75",(
R16833	T20865	T20863	arg3Of	),(
R16834	T20868	T20866	arg2Of	scenario,In
R16835	T20868	T20867	arg1Of	scenario,this
R16836	T20870	T20871	arg1Of	inhibition,of
R16837	T20870	T20874	arg1Of	inhibition,could
R16838	T20870	T20875	arg1Of	inhibition,lead
R16839	T20873	T20871	arg2Of	activity,of
R16840	T20873	T20872	arg1Of	activity,NF-κB
R16841	T20875	T20866	arg1Of	lead,In
R16842	T20875	T20869	arg1Of	lead,","
R16843	T20875	T20874	arg2Of	lead,could
R16844	T20875	T20876	arg1Of	lead,to
R16845	T20879	T20876	arg2Of	strategy,to
R16846	T20879	T20877	arg1Of	strategy,a
R16847	T20879	T20878	arg1Of	strategy,therapeutic
R16848	T20879	T20880	arg1Of	strategy,for
R16849	T20881	T20880	arg2Of	counteracting,for
R16850	T20884	T20881	arg2Of	production,counteracting
R16851	T20884	T20882	arg1Of	production,the
R16852	T20884	T20883	arg1Of	production,abnormal
R16853	T20884	T20885	arg1Of	production,of
R16854	T20884	T20890	arg1Of	production,in
R16855	T20887	T20886	arg1Of	cytokines,pro-inflammatory
R16856	T20887	T20888	arg1Of	cytokines,and
R16857	T20888	T20885	arg2Of	and,of
R16858	T20889	T20888	arg2Of	chemokines,and
R16859	T20892	T20890	arg2Of	infection,in
R16860	T20892	T20891	arg1Of	infection,HIV-1
R16861	T20894	T20893	arg1Of	inhibitors,NF-κB
R16862	T20894	T20895	arg1Of	inhibitors,","
R16863	T20894	T20897	arg1Of	inhibitors,as
R16864	T20894	T20906	arg1Of	inhibitors,reduced
R16865	T20897	T20896	arg1Of	as,such
R16866	T20898	T20899	arg1Of	anti-oxidants,","
R16867	T20899	T20901	arg1Of	",",and
R16868	T20900	T20899	arg2Of	proteasome,","
R16869	T20901	T20897	arg2Of	and,as
R16870	T20903	T20901	arg2Of	inhibitors,and
R16871	T20903	T20902	arg1Of	inhibitors,IKK
R16872	T20906	T20904	arg1Of	reduced,","
R16873	T20906	T20905	arg1Of	reduced,significantly
R16874	T20906	T20918	arg1Of	reduced,;
R16875	T20909	T20907	arg1Of	replication,the
R16876	T20909	T20908	arg1Of	replication,HIV-1
R16877	T20909	T20910	arg1Of	replication,and
R16878	T20910	T20906	arg2Of	and,reduced
R16879	T20914	T20910	arg2Of	response,and
R16880	T20914	T20911	arg1Of	response,the
R16881	T20914	T20912	arg2Of	response,associated
R16882	T20914	T20913	arg1Of	response,inflammatory
R16883	T20914	T20915	arg1Of	response,(
R16884	T20916	T20915	arg2Of	"76,77",(
R16885	T20917	T20915	arg3Of	),(
R16886	T20923	T20921	arg1Of	inhibitors,these
R16887	T20923	T20922	arg1Of	inhibitors,NF-κB
R16888	T20923	T20924	arg1Of	inhibitors,acted
R16889	T20924	T20918	arg2Of	acted,;
R16890	T20924	T20919	arg1Of	acted,however
R16891	T20924	T20920	arg1Of	acted,","
R16892	T20924	T20925	arg1Of	acted,indiscriminately
R16893	T20924	T20926	arg1Of	acted,in
R16894	T20928	T20929	arg1Of	HIV-1-infected,and
R16895	T20929	T20927	arg1Of	and,both
R16896	T20930	T20929	arg2Of	uninfected,and
R16897	T20931	T20926	arg2Of	cells,in
R16898	T20931	T20928	arg1Of	cells,HIV-1-infected
R16899	T20931	T20930	arg1Of	cells,uninfected
R16900	T20933	T20932	arg1Of	evidence,The
R16901	T20933	T20945	arg1Of	evidence,may
R16902	T20933	T20946	arg1Of	evidence,lead
R16903	T20935	T20936	arg1Of	Tat,activates
R16904	T20936	T20933	arg2Of	activates,evidence
R16905	T20936	T20934	arg1Of	activates,that
R16906	T20936	T20938	arg1Of	activates,through
R16907	T20937	T20936	arg2Of	NF-κB,activates
R16908	T20940	T20938	arg2Of	interaction,through
R16909	T20940	T20939	arg1Of	interaction,direct
R16910	T20940	T20941	arg1Of	interaction,with
R16911	T20942	T20943	arg1Of	IκB-α,and
R16912	T20943	T20941	arg2Of	and,with
R16913	T20944	T20943	arg2Of	p65,and
R16914	T20946	T20945	arg2Of	lead,may
R16915	T20946	T20947	arg1Of	lead,to
R16916	T20949	T20947	arg2Of	inhibitors,to
R16917	T20949	T20948	arg1Of	inhibitors,specific
R16918	T20949	T20950	modOf	inhibitors,to
R16919	T20951	T20950	arg1Of	counteract,to
R16920	T20955	T20951	arg2Of	action,counteract
R16921	T20955	T20952	arg1Of	action,the
R16922	T20955	T20953	arg1Of	action,Tat
R16923	T20955	T20954	arg1Of	action,pro-inflammatory
R19354	T24359	T24360	arg1Of	Tat,enhances
R19355	T24359	T24368	arg1Of	Tat,associating
R19356	T24359	T24372	arg1Of	Tat,counteracting
R19357	T24360	T24367	arg1Of	enhances,by
R19358	T24363	T24360	arg2Of	activity,enhances
R19359	T24363	T24361	arg1Of	activity,the
R19360	T24363	T24362	arg1Of	activity,NF-κB
R19361	T24363	T24364	arg1Of	activity,following
R19362	T24366	T24364	arg2Of	stimulation,following
R19363	T24366	T24365	arg1Of	stimulation,PMA
R19364	T24368	T24369	arg1Of	associating,with
R19365	T24368	T24371	arg1Of	associating,and
R19366	T24370	T24369	arg2Of	IκB-α,with
R19367	T24371	T24367	arg2Of	and,by
R19368	T24372	T24371	arg2Of	counteracting,and
R19369	T24375	T24372	arg2Of	repression,counteracting
R19370	T24375	T24373	arg1Of	repression,the
R19371	T24375	T24374	arg1Of	repression,NF-κB
R19372	T24377	T24376	arg1Of	cells,HeLa
R19373	T24377	T24378	arg1Of	cells,(
R19374	T24377	T24381	arg1Of	cells,were
R19375	T24377	T24382	arg2Of	cells,transfected
R19376	T24379	T24378	arg2Of	5 × 106,(
R19377	T24380	T24378	arg3Of	),(
R19378	T24382	T24381	arg2Of	transfected,were
R19379	T24382	T24383	arg1Of	transfected,with
R19380	T24382	T24393	arg1Of	transfected,and
R19381	T24384	T24385	arg1Of	p3xFLAG-Tat,or
R19382	T24385	T24383	arg2Of	or,with
R19383	T24388	T24385	arg2Of	vector,or
R19384	T24388	T24386	arg1Of	vector,p3xFLAG
R19385	T24388	T24387	arg1Of	vector,empty
R19386	T24388	T24389	arg1Of	vector,(
R19387	T24390	T24389	arg2Of	5 µg,(
R19388	T24391	T24389	arg3Of	),(
R19389	T24393	T24392	arg1Of	and,","
R19390	T24394	T24396	arg1Of	48 h,were
R19391	T24394	T24397	arg2Of	48 h,stimulated
R19392	T24394	T24406	arg1Of	48 h,left
R19393	T24394	T24409	arg1Of	48 h,washed
R19394	T24394	T24414	arg1Of	48 h,harvested
R19395	T24397	T24396	arg2Of	stimulated,were
R19396	T24397	T24398	arg1Of	stimulated,with
R19397	T24397	T24405	arg1Of	stimulated,or
R19398	T24399	T24398	arg2Of	PMA,with
R19399	T24399	T24400	arg1Of	PMA,(
R19400	T24401	T24400	arg2Of	5 min,(
R19401	T24401	T24402	arg1Of	5 min,","
R19402	T24403	T24402	arg2Of	20 ng/ml,","
R19403	T24404	T24400	arg3Of	),(
R19404	T24405	T24408	arg1Of	or,","
R19405	T24406	T24405	arg2Of	left,or
R19406	T24407	T24406	arg2Of	unstimulated,left
R19407	T24408	T24393	arg2Of	",",and
R19408	T24408	T24395	arg1Of	",",later
R19409	T24409	T24410	arg1Of	washed,twice
R19410	T24409	T24411	arg1Of	washed,with
R19411	T24409	T24413	arg1Of	washed,and
R19412	T24412	T24411	arg2Of	DMEM,with
R19413	T24413	T24408	arg2Of	and,","
R19414	T24414	T24413	arg2Of	harvested,and
R19415	T24414	T24415	arg1Of	harvested,at
R19416	T24418	T24415	arg2Of	times,at
R19417	T24418	T24416	arg1Of	times,the
R19418	T24418	T24417	arg1Of	times,indicated
R19419	T24419	T24420	arg1Of	Nuclear,and
R19420	T24421	T24420	arg2Of	cytosolic,and
R19421	T24422	T24419	arg1Of	extracts,Nuclear
R19422	T24422	T24421	arg1Of	extracts,cytosolic
R19423	T24422	T24423	arg1Of	extracts,were
R19424	T24422	T24424	arg2Of	extracts,prepared
R19425	T24424	T24423	arg2Of	prepared,were
R19426	T24424	T24425	arg1Of	prepared,for
R19427	T24427	T24425	arg2Of	analysis.,for
R19428	T24427	T24426	arg1Of	analysis.,further
R19429	T24427	T24433	modOf	analysis.,were
R19430	T24429	T24428	arg2Of	A,(
R19431	T24430	T24428	arg3Of	),(
R19432	T24432	T24428	arg1Of	extracts,(
R19433	T24432	T24431	arg1Of	extracts,Nuclear
R19434	T24432	T24433	arg1Of	extracts,were
R19435	T24432	T24434	arg2Of	extracts,analysed
R19436	T24434	T24433	arg2Of	analysed,were
R19437	T24434	T24435	arg1Of	analysed,for
R19438	T24437	T24438	arg1Of	p65,","
R19439	T24438	T24440	arg1Of	",",and
R19440	T24439	T24438	arg2Of	p50,","
R19441	T24441	T24440	arg2Of	FLAG-Tat,and
R19442	T24442	T24435	arg2Of	binding,for
R19443	T24442	T24436	arg1Of	binding,the
R19444	T24442	T24437	arg1Of	binding,p65
R19445	T24442	T24439	arg1Of	binding,p50
R19446	T24442	T24441	arg1Of	binding,FLAG-Tat
R19447	T24442	T24443	arg1Of	binding,to
R19448	T24447	T24443	arg2Of	oligonucleotide,to
R19449	T24447	T24444	arg1Of	oligonucleotide,the
R19450	T24447	T24445	arg1Of	oligonucleotide,NF-κB
R19451	T24447	T24446	arg1Of	oligonucleotide,double-stranded
R19452	T24447	T24448	arg1Of	oligonucleotide,using
R19453	T24455	T24449	arg1Of	kit,the
R19454	T24455	T24450	arg1Of	kit,NF-κB
R19455	T24455	T24451	arg1Of	kit,Transcription
R19456	T24455	T24452	arg1Of	kit,Factor
R19457	T24455	T24453	arg1Of	kit,ELISA
R19458	T24455	T24454	arg1Of	kit,assay
R19459	T24455	T24456	arg1Of	kit,(
R19460	T24455	T24469	modOf	kit,20 µ
R19461	T24455	T24480	arg1Of	kit,ester
R19462	T24455	T24488	arg1Of	kit,F
R19463	T24457	T24456	arg2Of	Cayman,(
R19464	T24458	T24456	arg3Of	),(
R19465	T24460	T24459	arg2Of	(,.
R19466	T24461	T24459	arg3Of	B,.
R19467	T24462	T24463	arg1Of	) Nucle,ar
R19468	T24464	T24463	arg2Of	and cytos,ar
R19469	T24465	T24459	arg1Of	olic ext,.
R19470	T24465	T24462	arg1Of	olic ext,) Nucle
R19471	T24465	T24464	arg1Of	olic ext,and cytos
R19472	T24465	T24466	arg1Of	olic ext,r
R19473	T24465	T24469	arg1Of	olic ext,20 µ
R19474	T24465	T24470	arg2Of	olic ext,g) were s
R19475	T24465	T24476	arg2Of	olic ext,SDS–PAGE
R19476	T24467	T24466	arg2Of	acts ,r
R19477	T24468	T24466	arg3Of	(,r
R19478	T24470	T24475	arg1Of	g) were s,2%
R19479	T24472	T24473	arg1Of	ar,a
R19480	T24474	T24470	arg1Of	ted by 1,g) were s
R19481	T24474	T24471	arg2Of	ted by 1,ep
R19482	T24474	T24472	arg1Of	ted by 1,ar
R19483	T24475	T24469	arg2Of	2% ,20 µ
R19484	T24476	T24475	arg2Of	SDS–PAGE,2%
R19485	T24479	T24476	arg1Of	sed by w,SDS–PAGE
R19486	T24479	T24477	arg2Of	sed by w,an
R19487	T24479	T24478	arg1Of	sed by w,d analy
R19488	T24482	T24483	arg1Of	lotting ,u
R19489	T24483	T24485	arg1Of	u,a
R19490	T24484	T24483	arg2Of	sing the ,u
R19491	T24486	T24485	arg2Of	"nti-p65, ant",a
R19492	T24487	T24480	arg2Of	i-,ester
R19493	T24487	T24481	arg1Of	i-,n b
R19494	T24487	T24482	arg1Of	i-,lotting
R19495	T24487	T24484	arg1Of	i-,sing the
R19496	T24487	T24486	arg1Of	i-,"nti-p65, ant"
R19497	T24488	T24448	arg2Of	F,using
R19498	T24489	T24490	arg1Of	"LAG, anti-",His
R19499	T24491	T24490	arg2Of	"tone H1, anti-IκB-",His
R19500	T24492	T24488	arg2Of	α and anti,F
R19501	T24492	T24489	arg1Of	α and anti,"LAG, anti-"
R19502	T24492	T24491	arg1Of	α and anti,"tone H1, anti-IκB-"
R19503	T24494	T24493	arg1Of	values,Densitometry
R19504	T24494	T24495	arg1Of	values,(
R19505	T24494	T24498	arg1Of	values,of
R19506	T24494	T24501	arg1Of	values,were
R19507	T24494	T24502	arg2Of	values,expressed
R19508	T24496	T24495	arg2Of	D,(
R19509	T24497	T24495	arg3Of	),(
R19510	T24500	T24498	arg2Of	bands,of
R19511	T24500	T24499	arg1Of	bands,the
R19512	T24502	T24501	arg2Of	expressed,were
R19513	T24502	T24503	arg1Of	expressed,as
R19514	T24502	T24554	arg1Of	expressed,t
R19515	T24505	T24503	arg2Of	increase,as
R19516	T24505	T24504	arg1Of	increase,fold
R19517	T24505	T24506	arg1Of	increase,above
R19518	T24505	T24518	modOf	increase,ity
R19519	T24508	T24506	arg2Of	control,above
R19520	T24508	T24507	arg1Of	control,the
R19521	T24510	T24509	arg2Of	lane,(
R19522	T24510	T24511	arg1Of	lane,1
R19523	T24512	T24509	arg3Of	),(
R19524	T24514	T24513	arg2Of	(,.
R19525	T24515	T24513	arg3Of	C,.
R19526	T24517	T24509	arg1Of	KK activ,(
R19527	T24517	T24513	arg1Of	KK activ,.
R19528	T24517	T24516	arg1Of	KK activ,) I
R19529	T24517	T24518	arg1Of	KK activ,ity
R19530	T24517	T24519	arg2Of	KK activ,was meas
R19531	T24519	T24518	arg2Of	was meas,ity
R19532	T24519	T24520	arg1Of	was meas,ur
R19533	T24519	T24526	arg1Of	was meas,10
R19534	T24522	T24520	arg2Of	osolic e,ur
R19535	T24522	T24521	arg1Of	osolic e,ed in cyt
R19536	T24522	T24523	arg1Of	osolic e,x
R19537	T24524	T24523	arg2Of	tracts,x
R19538	T24525	T24523	arg3Of	(,x
R19539	T24527	T24526	arg2Of	0 µg),10
R19540	T24531	T24527	arg2Of	IKK k,0 µg)
R19541	T24531	T24528	arg1Of	IKK k,by usi
R19542	T24531	T24529	arg1Of	IKK k,ng
R19543	T24531	T24530	arg1Of	IKK k,HTScan
R19544	T24531	T24532	arg1Of	IKK k,i
R19545	T24531	T24545	modOf	IKK k,cell
R19546	T24535	T24532	arg2Of	ll Signali,i
R19547	T24535	T24533	arg1Of	ll Signali,nase
R19548	T24535	T24534	arg1Of	ll Signali,assay (Ce
R19549	T24536	T24532	arg3Of	n,i
R19550	T24538	T24537	arg2Of	T,g
R19551	T24539	T24537	arg3Of	e,g
R19552	T24541	T24537	arg1Of	logy),g
R19553	T24541	T24540	arg1Of	logy),chno
R19554	T24541	T24542	arg1Of	logy),.
R19555	T24541	T24545	arg1Of	logy),cell
R19556	T24541	T24546	arg2Of	logy),s (5 × 106)
R19557	T24543	T24542	arg2Of	(D) HeL,.
R19558	T24544	T24542	arg3Of	a,.
R19559	T24546	T24545	arg2Of	s (5 × 106),cell
R19560	T24546	T24547	arg1Of	s (5 × 106),were
R19561	T24546	T24549	arg1Of	s (5 × 106),ansfect
R19562	T24547	T24548	arg1Of	were,tr
R19563	T24549	T24548	arg2Of	ansfect,tr
R19564	T24550	T24547	arg2Of	ed with or ,were
R19565	T24550	T24549	arg2Of	ed with or ,ansfect
R19566	T24550	T24551	arg1Of	ed with or ,w
R19567	T24552	T24551	arg2Of	itho,w
R19568	T24553	T24551	arg3Of	u,w
R19569	T24556	T24555	arg1Of	LAG-T,p3xF
R19570	T24558	T24559	arg1Of	t (5 ,µg);
R19571	T24558	T24560	arg2Of	t (5 ,48 h later
R19572	T24558	T24569	arg1Of	t (5 ,h PM
R19573	T24560	T24559	arg2Of	48 h later,µg);
R19574	T24560	T24561	arg1Of	48 h later,", ce"
R19575	T24560	T24568	arg1Of	48 h later,it
R19576	T24562	T24561	arg2Of	lls,", ce"
R19577	T24562	T24563	arg1Of	lls,w
R19578	T24564	T24563	arg2Of	ere s,w
R19579	T24564	T24565	arg1Of	ere s,t
R19580	T24566	T24565	arg2Of	imulated,t
R19581	T24567	T24563	arg3Of	w,w
R19582	T24568	T24554	arg2Of	it,t
R19583	T24568	T24556	arg1Of	it,LAG-T
R19584	T24568	T24557	arg1Of	it,a
R19585	T24569	T24568	arg2Of	h PM,it
R19586	T24569	T24571	arg1Of	h PM,
R19587	T24570	T24569	arg2Of	"A (5 min, 20",h PM
R19588	T24570	T24572	arg2Of	"A (5 min, 20",ng/ml)
R19589	T24570	T24578	arg2Of	"A (5 min, 20","ted, wash"
R19590	T24572	T24573	arg1Of	ng/ml),or le
R19591	T24572	T24574	arg1Of	ng/ml),ft u
R19592	T24572	T24577	arg1Of	ng/ml),ula
R19593	T24575	T24574	arg2Of	nsti,ft u
R19594	T24577	T24569	arg3Of	ula,h PM
R19595	T24577	T24576	arg1Of	ula,m
R19596	T24578	T24577	arg2Of	"ted, wash",ula
R19597	T24578	T24579	arg1Of	"ted, wash",ed tw
R19598	T24580	T24579	arg2Of	ice wit,ed tw
R19599	T24583	T24581	arg1Of	extracts,Whole
R19600	T24583	T24582	arg1Of	extracts,cell
R19601	T24583	T24584	arg1Of	extracts,(
R19602	T24583	T24587	arg1Of	extracts,were
R19603	T24583	T24588	arg2Of	extracts,immunoprecipitated
R19604	T24585	T24584	arg2Of	1 mg,(
R19605	T24586	T24584	arg3Of	),(
R19606	T24588	T24587	arg2Of	immunoprecipitated,were
R19607	T24588	T24589	arg1Of	immunoprecipitated,with
R19608	T24593	T24589	arg2Of	antibody,with
R19609	T24593	T24590	arg1Of	antibody,protein
R19610	T24593	T24591	arg1Of	antibody,G-Sepharose-coupled
R19611	T24593	T24592	arg1Of	antibody,anti-IκB-α
R19612	T24594	T24595	arg1Of	Immunocomplexes,were
R19613	T24594	T24596	arg2Of	Immunocomplexes,separated
R19614	T24594	T24602	arg2Of	Immunocomplexes,analysed
R19615	T24596	T24601	arg1Of	separated,and
R19616	T24598	T24599	arg1Of	12,%
R19617	T24600	T24596	arg1Of	SDS–PAGE,separated
R19618	T24600	T24597	arg2Of	SDS–PAGE,by
R19619	T24600	T24598	arg1Of	SDS–PAGE,12
R19620	T24601	T24595	arg2Of	and,were
R19621	T24602	T24601	arg2Of	analysed,and
R19622	T24605	T24602	arg1Of	blotting,analysed
R19623	T24605	T24603	arg2Of	blotting,by
R19624	T24605	T24604	arg1Of	blotting,western
R19625	T24605	T24606	arg1Of	blotting,with
R19626	T24607	T24608	arg1Of	anti-p65,","
R19627	T24608	T24610	arg1Of	",",and
R19628	T24609	T24608	arg2Of	anti-FLAG,","
R19629	T24611	T24610	arg2Of	anti-IκB-α,and
R19630	T24612	T24606	arg2Of	antibodies,with
R19631	T24612	T24607	arg1Of	antibodies,anti-p65
R19632	T24612	T24609	arg1Of	antibodies,anti-FLAG
R19633	T24612	T24611	arg1Of	antibodies,anti-IκB-α
R19634	T24614	T24613	arg1Of	values,Densitometry
R19635	T24614	T24615	arg1Of	values,(
R19636	T24614	T24618	arg1Of	values,of
R19637	T24614	T24621	arg1Of	values,were
R19638	T24614	T24622	arg2Of	values,expressed
R19639	T24616	T24615	arg2Of	D,(
R19640	T24617	T24615	arg3Of	),(
R19641	T24620	T24618	arg2Of	bands,of
R19642	T24620	T24619	arg1Of	bands,the
R19643	T24622	T24621	arg2Of	expressed,were
R19644	T24622	T24623	arg1Of	expressed,as
R19645	T24625	T24623	arg2Of	increase,as
R19646	T24625	T24624	arg1Of	increase,fold
R19647	T24625	T24626	arg1Of	increase,above
R19648	T24628	T24626	arg2Of	control,above
R19649	T24628	T24627	arg1Of	control,the
R19650	T24628	T24629	arg1Of	control,(
R19651	T24630	T24629	arg2Of	lane,(
R19652	T24630	T24631	arg1Of	lane,1
R19653	T24632	T24629	arg3Of	),(
R19654	T24633	T24634	arg1Of	Values,(
R19655	T24633	T24639	arg1Of	Values,are
R19656	T24633	T24640	arg2Of	Values,shown
R19657	T24635	T24634	arg2Of	mean ± SE,(
R19658	T24635	T24636	arg1Of	mean ± SE,","
R19659	T24637	T24636	arg2Of	n = 3,","
R19660	T24638	T24634	arg3Of	),(
R19661	T24640	T24639	arg2Of	shown,are
R20033	T25214	T25215	arg1Of	Tat,relieves
R20034	T25214	T25280	arg1Of	Tat,using
R20035	T25215	T25280	modOf	relieves,using
R20036	T25216	T25217	arg1Of	p65,from
R20037	T25216	T25226	arg1Of	p65,of
R20038	T25216	T25263	arg1Of	p65,","
R20039	T25222	T25221	arg2Of	A,(
R20040	T25223	T25221	arg3Of	),(
R20041	T25225	T25217	arg2Of	representation,from
R20042	T25225	T25218	arg1Of	representation,the
R20043	T25225	T25219	arg1Of	representation,IκB-α
R20044	T25225	T25220	arg1Of	representation,inhibition.
R20045	T25225	T25221	arg1Of	representation,(
R20046	T25225	T25224	arg1Of	representation,Schematic
R20047	T25227	T25228	arg1Of	wild-type,and
R20048	T25229	T25228	arg2Of	mutant,and
R20049	T25231	T25227	arg1Of	proteins.,wild-type
R20050	T25231	T25229	arg1Of	proteins.,mutant
R20051	T25231	T25230	arg1Of	proteins.,Tat
R20052	T25231	T25232	arg1Of	proteins.,(
R20053	T25231	T25239	modOf	proteins.,was
R20054	T25231	T25243	arg1Of	proteins.,or
R20055	T25233	T25232	arg2Of	B,(
R20056	T25234	T25232	arg3Of	),(
R20057	T25235	T25236	arg1Of	HA-IκB-α,(
R20058	T25235	T25239	arg1Of	HA-IκB-α,was
R20059	T25235	T25240	arg2Of	HA-IκB-α,incubated
R20060	T25237	T25236	arg2Of	5 µl,(
R20061	T25238	T25236	arg3Of	),(
R20062	T25240	T25239	arg2Of	incubated,was
R20063	T25240	T25241	arg1Of	incubated,in
R20064	T25242	T25241	arg2Of	presence,in
R20065	T25243	T25226	arg2Of	or,of
R20066	T25244	T25245	arg1Of	absence,of
R20067	T25244	T25256	arg1Of	absence,","
R20068	T25246	T25245	arg2Of	FLAG-Tat,of
R20069	T25246	T25247	arg1Of	FLAG-Tat,","
R20070	T25246	T25250	arg1Of	FLAG-Tat,","
R20071	T25249	T25247	arg2Of	T,","
R20072	T25249	T25248	arg1Of	T,FLAG-Tat
R20073	T25253	T25252	arg2Of	"23,24",(
R20074	T25254	T25252	arg3Of	),(
R20075	T25255	T25250	arg2Of	A,","
R20076	T25255	T25251	arg1Of	A,N
R20077	T25255	T25252	arg1Of	A,(
R20078	T25256	T25243	arg2Of	",",or
R20079	T25260	T25259	arg2Of	"50,51",(
R20080	T25261	T25259	arg3Of	),(
R20081	T25262	T25256	arg2Of	A,","
R20082	T25262	T25257	arg1Of	A,FLAG-Tat
R20083	T25262	T25258	arg1Of	A,K
R20084	T25262	T25259	arg1Of	A,(
R20085	T25263	T25270	arg1Of	",",or
R20086	T25265	T25264	arg1Of	R,FLAG-Tat
R20087	T25265	T25266	arg1Of	R,(
R20088	T25267	T25266	arg2Of	49–57,(
R20089	T25268	T25266	arg3Of	),(
R20090	T25269	T25263	arg2Of	A,","
R20091	T25269	T25265	arg1Of	A,R
R20092	T25270	T25215	arg2Of	or,relieves
R20093	T25274	T25273	arg2Of	"22,25,27",(
R20094	T25275	T25273	arg3Of	),(
R20095	T25276	T25270	arg2Of	A,or
R20096	T25276	T25271	arg1Of	A,FLAG-Tat
R20097	T25276	T25272	arg1Of	A,C
R20098	T25276	T25273	arg1Of	A,(
R20099	T25276	T25277	arg1Of	A,(
R20100	T25278	T25277	arg2Of	10 µl,(
R20101	T25279	T25277	arg3Of	),(
R20102	T25284	T25280	arg2Of	proteins,using
R20103	T25284	T25281	arg1Of	proteins,in
R20104	T25284	T25282	arg1Of	proteins,vitro
R20105	T25284	T25283	arg2Of	proteins,translated
R20106	T25285	T25286	arg1Of	Wild-type,and
R20107	T25287	T25286	arg2Of	mutant,and
R20108	T25289	T25285	arg1Of	proteins,Wild-type
R20109	T25289	T25287	arg1Of	proteins,mutant
R20110	T25289	T25288	arg1Of	proteins,Tat
R20111	T25289	T25290	arg1Of	proteins,were
R20112	T25289	T25291	arg2Of	proteins,immunoprecipitated
R20113	T25291	T25290	arg2Of	immunoprecipitated,were
R20114	T25291	T25292	arg1Of	immunoprecipitated,with
R20115	T25291	T25295	arg1Of	immunoprecipitated,;
R20116	T25294	T25292	arg2Of	antibody,with
R20117	T25294	T25293	arg1Of	antibody,anti-FLAG
R20118	T25296	T25297	arg1Of	immunocomplexes,were
R20119	T25296	T25298	arg2Of	immunocomplexes,separated
R20120	T25296	T25304	arg2Of	immunocomplexes,analysed
R20121	T25298	T25303	arg1Of	separated,and
R20122	T25300	T25301	arg1Of	12,%
R20123	T25302	T25298	arg1Of	SDS–PAGE,separated
R20124	T25302	T25299	arg2Of	SDS–PAGE,by
R20125	T25302	T25300	arg1Of	SDS–PAGE,12
R20126	T25303	T25295	arg2Of	and,;
R20127	T25303	T25297	arg2Of	and,were
R20128	T25303	T25308	arg1Of	and,with
R20129	T25304	T25303	arg2Of	analysed,and
R20130	T25307	T25304	arg1Of	blotting,analysed
R20131	T25307	T25305	arg2Of	blotting,by
R20132	T25307	T25306	arg1Of	blotting,western
R20133	T25309	T25310	arg1Of	anti-HA,and
R20134	T25311	T25310	arg2Of	anti-FLAG,and
R20135	T25312	T25308	arg2Of	antibodies.,with
R20136	T25312	T25309	arg1Of	antibodies.,anti-HA
R20137	T25312	T25311	arg1Of	antibodies.,anti-FLAG
R20138	T25312	T25313	arg1Of	antibodies.,(
R20139	T25312	T25321	modOf	antibodies.,was
R20140	T25314	T25313	arg2Of	C,(
R20141	T25315	T25313	arg3Of	),(
R20142	T25317	T25316	arg1Of	p65,35S-methionine-labeled
R20143	T25317	T25318	arg1Of	p65,(
R20144	T25317	T25321	arg1Of	p65,was
R20145	T25317	T25322	arg2Of	p65,incubated
R20146	T25319	T25318	arg2Of	5 µl,(
R20147	T25320	T25318	arg3Of	),(
R20148	T25322	T25321	arg2Of	incubated,was
R20149	T25322	T25323	arg1Of	incubated,with
R20150	T25325	T25324	arg1Of	vitro,in
R20151	T25327	T25323	arg2Of	HA-IκB-α,with
R20152	T25327	T25325	arg1Of	HA-IκB-α,vitro
R20153	T25327	T25326	arg2Of	HA-IκB-α,translated
R20154	T25327	T25328	arg1Of	HA-IκB-α,(
R20155	T25327	T25331	arg1Of	HA-IκB-α,in
R20156	T25327	T25335	arg1Of	HA-IκB-α,of
R20157	T25329	T25328	arg2Of	5 µl,(
R20158	T25330	T25328	arg3Of	),(
R20159	T25332	T25333	arg1Of	presence,or
R20160	T25333	T25331	arg2Of	or,in
R20161	T25334	T25333	arg2Of	absence,or
R20162	T25336	T25337	arg1Of	FLAG-Tat,or
R20163	T25339	T25337	arg2Of	R,or
R20164	T25339	T25338	arg1Of	R,FLAG-Tat
R20165	T25339	T25340	arg1Of	R,(
R20166	T25341	T25340	arg2Of	49–57,(
R20167	T25342	T25340	arg3Of	),(
R20168	T25343	T25335	arg2Of	A,of
R20169	T25343	T25336	arg1Of	A,FLAG-Tat
R20170	T25343	T25339	arg1Of	A,R
R20171	T25343	T25344	arg1Of	A,(
R20172	T25345	T25346	arg1Of	5,","
R20173	T25346	T25348	arg1Of	",",or
R20174	T25347	T25346	arg2Of	10,","
R20175	T25348	T25344	arg2Of	or,(
R20176	T25349	T25348	arg2Of	20 µl,or
R20177	T25350	T25344	arg3Of	),(
R20178	T25351	T25352	arg1Of	HA-IκB-α,was
R20179	T25351	T25353	arg2Of	HA-IκB-α,immunoprecipitated
R20180	T25353	T25352	arg2Of	immunoprecipitated,was
R20181	T25353	T25354	arg1Of	immunoprecipitated,with
R20182	T25353	T25357	arg1Of	immunoprecipitated,;
R20183	T25356	T25354	arg2Of	antibody,with
R20184	T25356	T25355	arg1Of	antibody,anti-HA
R20185	T25358	T25359	arg1Of	immunocomplexes,were
R20186	T25358	T25360	arg2Of	immunocomplexes,separated
R20187	T25358	T25366	arg2Of	immunocomplexes,analysed
R20188	T25360	T25365	arg1Of	separated,and
R20189	T25362	T25363	arg1Of	12,%
R20190	T25364	T25360	arg1Of	SDS–PAGE,separated
R20191	T25364	T25361	arg2Of	SDS–PAGE,by
R20192	T25364	T25362	arg1Of	SDS–PAGE,12
R20193	T25365	T25357	arg2Of	and,;
R20194	T25365	T25359	arg2Of	and,were
R20195	T25366	T25365	arg2Of	analysed,and
R20196	T25369	T25368	arg1Of	blotting,western
R20197	T25369	T25370	arg1Of	blotting,with
R20198	T25369	T25376	arg1Of	blotting,or
R20199	T25371	T25372	arg1Of	anti-HA,and
R20200	T25373	T25372	arg2Of	anti-FLAG,and
R20201	T25374	T25370	arg2Of	antibodies,with
R20202	T25374	T25371	arg1Of	antibodies,anti-HA
R20203	T25374	T25373	arg1Of	antibodies,anti-FLAG
R20204	T25376	T25366	arg1Of	or,analysed
R20205	T25376	T25367	arg2Of	or,by
R20206	T25376	T25375	arg1Of	or,","
R20207	T25377	T25376	arg2Of	autoradiography,or
R20208	T25377	T25378	arg1Of	autoradiography,(
R20209	T25379	T25378	arg2Of	35S-Met-p65,(
R20210	T25380	T25378	arg3Of	),(
R20211	T25382	T25381	arg1Of	values,Densitometry
R20212	T25382	T25383	arg1Of	values,(
R20213	T25382	T25386	arg1Of	values,of
R20214	T25382	T25389	arg1Of	values,were
R20215	T25382	T25390	arg2Of	values,expressed
R20216	T25384	T25383	arg2Of	D,(
R20217	T25385	T25383	arg3Of	),(
R20218	T25388	T25386	arg2Of	bands,of
R20219	T25388	T25387	arg1Of	bands,the
R20220	T25390	T25389	arg2Of	expressed,were
R20221	T25390	T25391	arg1Of	expressed,as
R20222	T25393	T25391	arg2Of	increase,as
R20223	T25393	T25392	arg1Of	increase,fold
R20224	T25393	T25394	arg1Of	increase,above
R20225	T25393	T25401	arg1Of	increase,.
R20226	T25396	T25394	arg2Of	control,above
R20227	T25396	T25395	arg1Of	control,the
R20228	T25396	T25397	arg1Of	control,(
R20229	T25398	T25397	arg2Of	lane,(
R20230	T25398	T25399	arg1Of	lane,1
R20231	T25400	T25397	arg3Of	),(
R20232	T25402	T25401	arg2Of	(,.
R20233	T25403	T25401	arg3Of	D,.
R20234	T25408	T25404	arg1Of	ding act,) T
R20235	T25408	T25405	arg1Of	ding act,he
R20236	T25408	T25406	arg1Of	ding act,p65
R20237	T25408	T25407	arg1Of	ding act,DNA bin
R20238	T25408	T25409	arg1Of	ding act,ivi
R20239	T25408	T25410	arg2Of	ding act,ty was a
R20240	T25410	T25389	modOf	ty was a,were
R20241	T25410	T25409	arg2Of	ty was a,ivi
R20242	T25412	T25410	arg1Of	lyse,ty was a
R20243	T25412	T25411	arg2Of	lyse,na
R20244	T25412	T25413	arg1Of	lyse,d by
R20245	T25415	T25414	arg1Of	SA us,EM
R20246	T25417	T25413	arg2Of	ro trans,d by
R20247	T25417	T25415	arg1Of	ro trans,SA us
R20248	T25417	T25416	arg2Of	ro trans,ing in vit
R20249	T25420	T25418	arg1Of	oligonucleotide,32P-labeled-NF-κB
R20250	T25420	T25419	arg1Of	oligonucleotide,double-stranded
R20251	T25420	T25421	arg1Of	oligonucleotide,was
R20252	T25420	T25422	arg2Of	oligonucleotide,incubated
R20253	T25422	T25421	arg2Of	incubated,was
R20254	T25422	T25423	arg1Of	incubated,with
R20255	T25422	T25451	arg1Of	incubated,;
R20256	T25424	T25423	arg2Of	p65,with
R20257	T25424	T25425	arg1Of	p65,(
R20258	T25424	T25428	arg1Of	p65,in
R20259	T25424	T25432	arg1Of	p65,of
R20260	T25426	T25425	arg2Of	0.5 µl,(
R20261	T25427	T25425	arg3Of	),(
R20262	T25429	T25430	arg1Of	presence,or
R20263	T25430	T25428	arg2Of	or,in
R20264	T25431	T25430	arg2Of	absence,or
R20265	T25433	T25434	arg1Of	HA-IκB-α,(
R20266	T25433	T25437	arg1Of	HA-IκB-α,","
R20267	T25435	T25434	arg2Of	1 µl,(
R20268	T25436	T25434	arg3Of	),(
R20269	T25437	T25439	arg1Of	",",or
R20270	T25438	T25437	arg2Of	FLAG-Tat,","
R20271	T25439	T25432	arg2Of	or,of
R20272	T25441	T25440	arg1Of	R,FLAG-Tat
R20273	T25441	T25442	arg1Of	R,(
R20274	T25443	T25442	arg2Of	49–57,(
R20275	T25444	T25442	arg3Of	),(
R20276	T25445	T25439	arg2Of	A,or
R20277	T25445	T25441	arg1Of	A,R
R20278	T25445	T25446	arg1Of	A,(
R20279	T25447	T25448	arg1Of	5,and
R20280	T25448	T25446	arg2Of	and,(
R20281	T25449	T25448	arg2Of	10 µl,and
R20282	T25450	T25446	arg3Of	),(
R20283	T25452	T25453	arg1Of	competition,of
R20284	T25452	T25456	arg1Of	competition,was
R20285	T25452	T25457	arg2Of	competition,performed
R20286	T25455	T25453	arg2Of	binding,of
R20287	T25455	T25454	arg1Of	binding,DNA
R20288	T25457	T25451	arg2Of	performed,;
R20289	T25457	T25456	arg2Of	performed,was
R20290	T25457	T25458	arg1Of	performed,with
R20291	T25461	T25460	arg1Of	to,up
R20292	T25464	T25458	arg2Of	excess,with
R20293	T25464	T25459	arg1Of	excess,10 -
R20294	T25464	T25461	arg1Of	excess,to
R20295	T25464	T25462	arg1Of	excess,100-fold
R20296	T25464	T25463	arg1Of	excess,molar
R20297	T25464	T25465	arg1Of	excess,of
R20298	T25469	T25465	arg2Of	oligonucleotide,of
R20299	T25469	T25466	arg1Of	oligonucleotide,unlabeled
R20300	T25469	T25467	arg1Of	oligonucleotide,NF-κB
R20301	T25469	T25468	arg1Of	oligonucleotide,double-stranded
R20302	T25471	T25470	arg1Of	complexes,DNA/protein
R20303	T25471	T25472	arg1Of	complexes,were
R20304	T25471	T25473	arg2Of	complexes,run
R20305	T25471	T25479	arg2Of	complexes,analysed
R20306	T25473	T25474	arg1Of	run,on
R20307	T25473	T25478	arg1Of	run,and
R20308	T25475	T25476	arg1Of	6,%
R20309	T25477	T25474	arg2Of	PAGE–TBE,on
R20310	T25477	T25475	arg1Of	PAGE–TBE,6
R20311	T25478	T25472	arg2Of	and,were
R20312	T25479	T25478	arg2Of	analysed,and
R20313	T25481	T25479	arg1Of	autoradiography.,analysed
R20314	T25481	T25480	arg2Of	autoradiography.,by
R20315	T25481	T25482	arg1Of	autoradiography.,(
R20316	T25481	T25490	modOf	autoradiography.,were
R20317	T25483	T25482	arg2Of	E,(
R20318	T25484	T25482	arg3Of	),(
R20319	T25485	T25486	arg1Of	p50−/−p65−/−MEFs,(
R20320	T25485	T25490	arg1Of	p50−/−p65−/−MEFs,were
R20321	T25485	T25491	arg2Of	p50−/−p65−/−MEFs,transfected
R20322	T25488	T25486	arg2Of	cells,(
R20323	T25488	T25487	arg1Of	cells,3 × 105
R20324	T25489	T25486	arg3Of	),(
R20325	T25491	T25490	arg2Of	transfected,were
R20326	T25491	T25492	arg1Of	transfected,with
R20327	T25493	T25494	arg1Of	pκBLuc,(
R20328	T25493	T25497	arg1Of	pκBLuc,and
R20329	T25495	T25494	arg2Of	0.5 µg,(
R20330	T25496	T25494	arg3Of	),(
R20331	T25497	T25492	arg2Of	and,with
R20332	T25498	T25499	arg1Of	pSV-β-Gal,(
R20333	T25498	T25502	arg1Of	pSV-β-Gal,with
R20334	T25498	T25504	arg1Of	pSV-β-Gal,without
R20335	T25500	T25499	arg2Of	0.1 µg,(
R20336	T25501	T25499	arg3Of	),(
R20337	T25502	T25503	arg1Of	with,or
R20338	T25504	T25503	arg2Of	without,or
R20339	T25505	T25506	arg1Of	pRc/CMV-p65,(
R20340	T25505	T25509	arg1Of	pRc/CMV-p65,","
R20341	T25507	T25506	arg2Of	0.5 µg,(
R20342	T25508	T25506	arg3Of	),(
R20343	T25509	T25514	arg1Of	",",","
R20344	T25510	T25509	arg2Of	pCMV4-HA-IκB-α,","
R20345	T25510	T25511	arg1Of	pCMV4-HA-IκB-α,(
R20346	T25512	T25511	arg2Of	0.5 µg,(
R20347	T25513	T25511	arg3Of	),(
R20348	T25514	T25516	arg1Of	",",or
R20349	T25515	T25514	arg2Of	p3xFLAG-Tat,","
R20350	T25516	T25502	arg2Of	or,with
R20351	T25516	T25504	arg2Of	or,without
R20352	T25518	T25516	arg2Of	R,or
R20353	T25518	T25517	arg1Of	R,p3xFLAG-Tat
R20354	T25518	T25519	arg1Of	R,(
R20355	T25520	T25519	arg2Of	49–57,(
R20356	T25521	T25519	arg3Of	),(
R20357	T25522	T25497	arg2Of	A,and
R20358	T25522	T25498	arg1Of	A,pSV-β-Gal
R20359	T25522	T25523	arg1Of	A,(
R20360	T25524	T25525	arg1Of	0.5,","
R20361	T25525	T25527	arg1Of	",",and
R20362	T25526	T25525	arg2Of	1,","
R20363	T25527	T25523	arg2Of	and,(
R20364	T25528	T25527	arg2Of	2 µg,and
R20365	T25529	T25523	arg3Of	),(
R20366	T25532	T25530	arg1Of	activity,The
R20367	T25532	T25531	arg1Of	activity,luciferase
R20368	T25532	T25533	arg1Of	activity,was
R20369	T25532	T25534	arg2Of	activity,measured
R20370	T25532	T25541	arg2Of	activity,normalized
R20371	T25534	T25535	arg1Of	measured,in
R20372	T25534	T25540	arg1Of	measured,and
R20373	T25537	T25535	arg2Of	extracts,in
R20374	T25537	T25536	arg1Of	extracts,cell
R20375	T25537	T25539	arg1Of	extracts,post-transfection
R20376	T25539	T25538	arg1Of	post-transfection,48-h
R20377	T25540	T25533	arg2Of	and,was
R20378	T25541	T25540	arg2Of	normalized,and
R20379	T25541	T25542	arg1Of	normalized,to
R20380	T25544	T25542	arg2Of	activity,to
R20381	T25544	T25543	arg1Of	activity,β-galactosidase
R20382	T25546	T25545	arg1Of	activation,Fold
R20383	T25546	T25547	arg1Of	activation,was
R20384	T25546	T25548	arg2Of	activation,calculated
R20385	T25548	T25547	arg2Of	calculated,was
R20386	T25548	T25550	arg1Of	calculated,to
R20387	T25550	T25549	arg1Of	to,relative
R20388	T25551	T25550	arg2Of	transfection,to
R20389	T25551	T25552	arg1Of	transfection,of
R20390	T25555	T25552	arg2Of	plasmid,of
R20391	T25555	T25553	arg1Of	plasmid,the
R20392	T25555	T25554	arg1Of	plasmid,pκBLuc
R20393	T25555	T25556	arg1Of	plasmid,alone
R20394	T25557	T25558	arg1Of	Values,(
R20395	T25557	T25563	arg1Of	Values,are
R20396	T25557	T25564	arg2Of	Values,shown
R20397	T25559	T25558	arg2Of	mean ± SE,(
R20398	T25559	T25560	arg1Of	mean ± SE,","
R20399	T25561	T25560	arg2Of	n = 3,","
R20400	T25562	T25558	arg3Of	),(
R20401	T25564	T25563	arg2Of	shown,are
R20402	T25566	T25565	arg2Of	control,As
R20403	T25566	T25567	arg1Of	control,of
R20404	T25569	T25567	arg2Of	expression,of
R20405	T25569	T25568	arg1Of	expression,protein
R20406	T25571	T25572	arg1Of	aliquots,of
R20407	T25571	T25578	arg1Of	aliquots,were
R20408	T25571	T25579	arg2Of	aliquots,analysed
R20409	T25574	T25572	arg2Of	extracts,of
R20410	T25574	T25573	arg1Of	extracts,cell
R20411	T25574	T25575	arg1Of	extracts,(
R20412	T25576	T25575	arg2Of	20 µg,(
R20413	T25577	T25575	arg3Of	),(
R20414	T25579	T25565	arg1Of	analysed,As
R20415	T25579	T25570	arg1Of	analysed,","
R20416	T25579	T25578	arg2Of	analysed,were
R20417	T25582	T25579	arg1Of	blotting,analysed
R20418	T25582	T25580	arg2Of	blotting,by
R20419	T25582	T25581	arg1Of	blotting,western
R20420	T25582	T25583	arg1Of	blotting,with
R20421	T25584	T25585	arg1Of	anti-p65,","
R20422	T25585	T25587	arg1Of	",",","
R20423	T25586	T25585	arg2Of	anti-HA,","
R20424	T25587	T25589	arg1Of	",",and
R20425	T25588	T25587	arg2Of	anti-FLAG,","
R20426	T25590	T25589	arg2Of	anti-γ-tubulin,and
R20427	T25591	T25583	arg2Of	antibodies,with
R20428	T25591	T25584	arg1Of	antibodies,anti-p65
R20429	T25591	T25586	arg1Of	antibodies,anti-HA
R20430	T25591	T25588	arg1Of	antibodies,anti-FLAG
R20431	T25591	T25590	arg1Of	antibodies,anti-γ-tubulin
R21360	T27266	T27280	arg1Of	Tat,promoting
R21368	T27275	T27279	arg1Of	occupying,and
R21369	T27278	T27275	arg2Of	enhancers,occupying
R21370	T27278	T27276	arg1Of	enhancers,the
R21371	T27278	T27277	arg1Of	enhancers,NF-κB
R21373	T27280	T27279	arg2Of	promoting,and
R21374	T27283	T27280	arg2Of	recruitment,promoting
R21375	T27283	T27281	arg1Of	recruitment,the
R21376	T27283	T27282	arg1Of	recruitment,p65
R21377	T27283	T27284	arg1Of	recruitment,with
R21378	T27286	T27284	arg2Of	displacement,with
R21379	T27286	T27285	arg1Of	displacement,IκB-α
R21380	T27288	T27287	arg1Of	cells,HeLa
R21381	T27288	T27289	arg1Of	cells,(
R21382	T27288	T27292	arg1Of	cells,were
R21383	T27288	T27293	arg2Of	cells,transfected
R21384	T27290	T27289	arg2Of	5 × 106,(
R21385	T27291	T27289	arg3Of	),(
R21386	T27293	T27292	arg2Of	transfected,were
R21387	T27293	T27294	arg1Of	transfected,with
R21388	T27293	T27314	arg1Of	transfected,;
R21389	T27295	T27296	arg1Of	p3xFLAG-Tat,","
R21390	T27296	T27302	arg1Of	",",","
R21391	T27298	T27296	arg2Of	C,","
R21392	T27298	T27297	arg1Of	C,p3xFLAG-Tat
R21393	T27298	T27299	arg1Of	C,(
R21394	T27300	T27299	arg2Of	"22,25,27",(
R21395	T27301	T27299	arg3Of	),(
R21396	T27302	T27308	arg1Of	",",or
R21397	T27304	T27302	arg2Of	R,","
R21398	T27304	T27303	arg1Of	R,p3xFLAG-Tat
R21399	T27304	T27305	arg1Of	R,(
R21400	T27306	T27305	arg2Of	49–57,(
R21401	T27307	T27305	arg3Of	),(
R21402	T27308	T27294	arg2Of	or,with
R21403	T27310	T27308	arg2Of	vector,or
R21404	T27310	T27309	arg1Of	vector,empty
R21405	T27310	T27311	arg1Of	vector,(
R21406	T27312	T27311	arg2Of	10 µg,(
R21407	T27313	T27311	arg3Of	),(
R21408	T27318	T27315	arg2Of	interference,for
R21409	T27318	T27316	arg1Of	interference,p65
R21410	T27318	T27317	arg1Of	interference,RNA
R21411	T27320	T27321	arg1Of	cells,(
R21412	T27320	T27324	arg1Of	cells,were
R21413	T27320	T27325	arg2Of	cells,transfected
R21414	T27322	T27321	arg2Of	5 × 106,(
R21415	T27323	T27321	arg3Of	),(
R21416	T27325	T27314	arg2Of	transfected,;
R21417	T27325	T27315	arg1Of	transfected,for
R21418	T27325	T27319	arg1Of	transfected,","
R21419	T27325	T27324	arg2Of	transfected,were
R21420	T27325	T27326	arg1Of	transfected,with
R21421	T27327	T27328	arg1Of	p3xFLAG-Tat,(
R21422	T27327	T27331	arg1Of	p3xFLAG-Tat,and
R21423	T27329	T27328	arg2Of	10 µg,(
R21424	T27330	T27328	arg3Of	),(
R21425	T27331	T27334	arg1Of	and,or
R21426	T27333	T27331	arg2Of	p65,and
R21427	T27333	T27332	arg1Of	p65,siRNA
R21428	T27334	T27326	arg2Of	or,with
R21429	T27336	T27334	arg2Of	control,or
R21430	T27336	T27335	arg1Of	control,siRNA
R21431	T27336	T27337	arg1Of	control,(
R21432	T27338	T27337	arg2Of	200 pmol,(
R21433	T27339	T27337	arg3Of	),(
R21434	T27341	T27342	arg1Of	post-transfection,","
R21435	T27343	T27342	arg2Of	total,","
R21436	T27344	T27340	arg1Of	RNA,Twenty-four-hour
R21437	T27344	T27341	arg1Of	RNA,post-transfection
R21438	T27344	T27343	arg1Of	RNA,total
R21439	T27344	T27345	arg1Of	RNA,was
R21440	T27344	T27346	arg2Of	RNA,extracted
R21441	T27344	T27348	arg2Of	RNA,analysed
R21442	T27346	T27347	arg1Of	extracted,and
R21443	T27347	T27345	arg2Of	and,was
R21444	T27347	T27352	modOf	and,to
R21445	T27348	T27347	arg2Of	analysed,and
R21446	T27351	T27346	arg1Of	PCR,extracted
R21447	T27351	T27348	arg1Of	PCR,analysed
R21448	T27351	T27349	arg2Of	PCR,by
R21449	T27351	T27350	arg1Of	PCR,real-time
R21450	T27353	T27352	arg1Of	evaluate,to
R21451	T27355	T27354	arg1Of	expression,the
R21452	T27355	T27356	arg1Of	expression,of
R21453	T27355	T27415	arg1Of	expression,was
R21454	T27355	T27416	arg2Of	expression,performed
R21455	T27357	T27358	arg1Of	MIP-1α,(
R21456	T27357	T27361	arg1Of	MIP-1α,","
R21457	T27359	T27358	arg2Of	A,(
R21458	T27360	T27358	arg3Of	),(
R21459	T27361	T27366	arg1Of	",",","
R21460	T27362	T27361	arg2Of	CSF3,","
R21461	T27362	T27363	arg1Of	CSF3,(
R21462	T27364	T27363	arg2Of	B,(
R21463	T27365	T27363	arg3Of	),(
R21464	T27366	T27371	arg1Of	",",","
R21465	T27367	T27366	arg2Of	LTA,","
R21466	T27367	T27368	arg1Of	LTA,(
R21467	T27369	T27368	arg2Of	C,(
R21468	T27370	T27368	arg3Of	),(
R21469	T27371	T27356	arg2Of	",",of
R21470	T27372	T27373	arg1Of	NFKBIA,(
R21471	T27372	T27376	arg1Of	NFKBIA,","
R21472	T27374	T27373	arg2Of	D,(
R21473	T27375	T27373	arg3Of	),(
R21474	T27376	T27381	arg1Of	",",","
R21475	T27377	T27376	arg2Of	TLR2,","
R21476	T27377	T27378	arg1Of	TLR2,(
R21477	T27379	T27378	arg2Of	E,(
R21478	T27380	T27378	arg3Of	),(
R21479	T27381	T27386	arg1Of	",",","
R21480	T27382	T27381	arg2Of	GAPDH,","
R21481	T27382	T27383	arg1Of	GAPDH,(
R21482	T27384	T27383	arg2Of	F,(
R21483	T27385	T27383	arg3Of	),(
R21484	T27386	T27371	arg2Of	",",","
R21485	T27386	T27400	modOf	",",were
R21486	T27387	T27386	arg2Of	ACTB,","
R21487	T27387	T27388	arg1Of	ACTB,(
R21488	T27389	T27388	arg2Of	G,(
R21489	T27390	T27388	arg3Of	),(
R21490	T27393	T27392	arg2Of	H,(
R21491	T27394	T27392	arg3Of	),(
R21492	T27396	T27391	arg1Of	cells,genes.
R21493	T27396	T27392	arg1Of	cells,(
R21494	T27396	T27395	arg1Of	cells,HeLa
R21495	T27396	T27397	arg1Of	cells,(
R21496	T27396	T27400	arg1Of	cells,were
R21497	T27396	T27401	arg2Of	cells,transfected
R21498	T27398	T27397	arg2Of	5 × 106,(
R21499	T27399	T27397	arg3Of	),(
R21500	T27401	T27400	arg2Of	transfected,were
R21501	T27401	T27402	arg1Of	transfected,with
R21502	T27403	T27405	arg1Of	p3xFLAG-Tat,or
R21503	T27405	T27402	arg2Of	or,with
R21504	T27405	T27404	arg1Of	or,","
R21505	T27407	T27406	arg1Of	vector,empty
R21506	T27407	T27408	arg1Of	vector,(
R21507	T27407	T27411	arg1Of	vector,and
R21508	T27409	T27408	arg2Of	10 µg,(
R21509	T27410	T27408	arg3Of	),(
R21510	T27411	T27405	arg2Of	and,or
R21511	T27414	T27411	arg2Of	ChIP,and
R21512	T27414	T27412	arg1Of	ChIP,48 h
R21513	T27414	T27413	arg1Of	ChIP,later
R21514	T27416	T27353	arg2Of	performed,evaluate
R21515	T27416	T27415	arg2Of	performed,was
R21516	T27416	T27417	arg1Of	performed,with
R21517	T27419	T27417	arg2Of	antibody,with
R21518	T27419	T27418	arg1Of	antibody,anti-p65
R21519	T27421	T27420	arg1Of	PCR,Real-time
R21520	T27421	T27422	arg1Of	PCR,was
R21521	T27421	T27423	arg2Of	PCR,performed
R21522	T27423	T27422	arg2Of	performed,was
R21523	T27423	T27424	arg1Of	performed,with
R21524	T27423	T27464	arg1Of	performed,;
R21525	T27425	T27424	arg2Of	primers,with
R21526	T27425	T27426	arg1Of	primers,specific
R21527	T27425	T27439	modOf	primers,were
R21528	T27426	T27427	arg1Of	specific,for
R21529	T27430	T27427	arg2Of	promoters.,for
R21530	T27430	T27428	arg1Of	promoters.,the
R21531	T27430	T27429	arg2Of	promoters.,indicated
R21532	T27432	T27431	arg2Of	I,(
R21533	T27433	T27431	arg3Of	),(
R21534	T27435	T27431	arg1Of	cells,(
R21535	T27435	T27434	arg1Of	cells,HeLa
R21536	T27435	T27436	arg1Of	cells,(
R21537	T27435	T27439	arg1Of	cells,were
R21538	T27435	T27440	arg2Of	cells,transfected
R21539	T27437	T27436	arg2Of	5 × 106,(
R21540	T27438	T27436	arg3Of	),(
R21541	T27440	T27439	arg2Of	transfected,were
R21542	T27440	T27441	arg1Of	transfected,with
R21543	T27443	T27442	arg1Of	vector,empty
R21544	T27443	T27445	arg1Of	vector,or
R21545	T27445	T27444	arg1Of	or,","
R21546	T27445	T27458	arg1Of	or,or
R21547	T27446	T27445	arg2Of	p3xFLAG-Tat,or
R21548	T27446	T27447	arg1Of	p3xFLAG-Tat,(
R21549	T27446	T27450	arg1Of	p3xFLAG-Tat,in
R21550	T27448	T27447	arg2Of	10 µg,(
R21551	T27449	T27447	arg3Of	),(
R21552	T27451	T27452	arg1Of	presence,or
R21553	T27452	T27450	arg2Of	or,in
R21554	T27452	T27454	arg1Of	or,of
R21555	T27453	T27452	arg2Of	absence,or
R21556	T27456	T27454	arg2Of	p65,of
R21557	T27456	T27455	arg1Of	p65,siRNA
R21558	T27458	T27441	arg2Of	or,with
R21559	T27458	T27457	arg1Of	or,","
R21560	T27460	T27458	arg2Of	control,or
R21561	T27460	T27459	arg1Of	control,siRNA
R21562	T27460	T27461	arg1Of	control,(
R21563	T27462	T27461	arg2Of	200 pmol,(
R21564	T27463	T27461	arg3Of	),(
R21565	T27466	T27465	arg1Of	later,48 h
R21566	T27468	T27469	arg1Of	ChIP,was
R21567	T27468	T27470	arg2Of	ChIP,performed
R21568	T27470	T27464	arg2Of	performed,;
R21569	T27470	T27466	arg1Of	performed,later
R21570	T27470	T27467	arg1Of	performed,","
R21571	T27470	T27469	arg2Of	performed,was
R21572	T27470	T27471	arg1Of	performed,with
R21573	T27473	T27471	arg2Of	antibody,with
R21574	T27473	T27472	arg1Of	antibody,anti-FLAG
R21575	T27475	T27474	arg1Of	PCR,Real-time
R21576	T27475	T27476	arg1Of	PCR,was
R21577	T27475	T27477	arg2Of	PCR,performed
R21578	T27477	T27476	arg2Of	performed,was
R21579	T27477	T27478	arg1Of	performed,with
R21580	T27479	T27478	arg2Of	primers,with
R21581	T27479	T27480	arg1Of	primers,specific
R21582	T27480	T27481	arg1Of	specific,for
R21583	T27484	T27481	arg2Of	promoters.,for
R21584	T27484	T27482	arg1Of	promoters.,the
R21585	T27484	T27483	arg2Of	promoters.,indicated
R21586	T27484	T27485	arg1Of	promoters.,(
R21587	T27486	T27485	arg2Of	J,(
R21588	T27487	T27485	arg3Of	),(
R21589	T27489	T27488	arg1Of	cells,HeLa
R21590	T27489	T27490	arg1Of	cells,(
R21591	T27489	T27493	arg1Of	cells,were
R21592	T27489	T27494	arg2Of	cells,transfected
R21593	T27491	T27490	arg2Of	5 × 106,(
R21594	T27492	T27490	arg3Of	),(
R21595	T27494	T27476	modOf	transfected,was
R21596	T27494	T27493	arg2Of	transfected,were
R21597	T27494	T27495	arg1Of	transfected,with
R21598	T27494	T27516	arg1Of	transfected,;
R21599	T27497	T27496	arg1Of	vector,empty
R21600	T27497	T27498	arg1Of	vector,","
R21601	T27498	T27504	arg1Of	",",or
R21602	T27499	T27498	arg2Of	p3xFLAG-Tat,","
R21603	T27499	T27500	arg1Of	p3xFLAG-Tat,(
R21604	T27501	T27500	arg2Of	10 µg,(
R21605	T27502	T27500	arg3Of	),(
R21606	T27504	T27503	arg1Of	or,","
R21607	T27504	T27510	arg1Of	or,or
R21608	T27506	T27505	arg1Of	vector,empty
R21609	T27506	T27507	arg1Of	vector,plus
R21610	T27507	T27504	arg2Of	plus,or
R21611	T27509	T27507	arg2Of	IκB-α,plus
R21612	T27509	T27508	arg1Of	IκB-α,siRNA
R21613	T27510	T27495	arg2Of	or,with
R21614	T27512	T27510	arg2Of	control,or
R21615	T27512	T27511	arg1Of	control,siRNA
R21616	T27512	T27513	arg1Of	control,(
R21617	T27514	T27513	arg2Of	200 pmol,(
R21618	T27515	T27513	arg3Of	),(
R21619	T27518	T27517	arg1Of	later,48 h
R21620	T27520	T27521	arg1Of	ChIP,was
R21621	T27520	T27522	arg2Of	ChIP,performed
R21622	T27522	T27516	arg2Of	performed,;
R21623	T27522	T27518	arg1Of	performed,later
R21624	T27522	T27519	arg1Of	performed,","
R21625	T27522	T27521	arg2Of	performed,was
R21626	T27522	T27523	arg1Of	performed,with
R21627	T27525	T27523	arg2Of	antibody,with
R21628	T27525	T27524	arg1Of	antibody,anti-IκB-α
R21629	T27527	T27526	arg1Of	PCR,Real-time
R21630	T27527	T27528	arg1Of	PCR,was
R21631	T27527	T27529	arg2Of	PCR,performed
R21632	T27529	T27528	arg2Of	performed,was
R21633	T27529	T27530	arg1Of	performed,with
R21634	T27531	T27530	arg2Of	primers,with
R21635	T27531	T27532	arg1Of	primers,specific
R21636	T27532	T27533	arg1Of	specific,for
R21637	T27536	T27533	arg2Of	promoters,for
R21638	T27536	T27534	arg1Of	promoters,the
R21639	T27536	T27535	arg1Of	promoters,indicated
R21640	T27537	T27538	arg1Of	Values,(
R21641	T27537	T27543	arg1Of	Values,are
R21642	T27537	T27544	arg2Of	Values,shown
R21643	T27539	T27538	arg2Of	mean ± SE,(
R21644	T27539	T27540	arg1Of	mean ± SE,","
R21645	T27541	T27540	arg2Of	n = 3,","
R21646	T27542	T27538	arg3Of	),(
R21647	T27544	T27543	arg2Of	shown,are
R21648	T27546	T27545	arg1Of	asterisks,The
R21649	T27546	T27547	arg1Of	asterisks,indicate
R21650	T27547	T27559	arg1Of	indicate,according
R21651	T27549	T27548	arg1Of	significant,statistically
R21652	T27550	T27547	arg2Of	differences,indicate
R21653	T27550	T27549	arg1Of	differences,significant
R21654	T27550	T27551	arg1Of	differences,compared
R21655	T27552	T27551	arg2Of	to,compared
R21656	T27554	T27552	arg2Of	control,to
R21657	T27554	T27553	arg1Of	control,the
R21658	T27554	T27555	arg1Of	control,(
R21659	T27557	T27555	arg2Of	vector,(
R21660	T27557	T27556	arg1Of	vector,empty
R21661	T27558	T27555	arg3Of	),(
R21662	T27560	T27559	arg2Of	to,according
R21663	T27562	T27561	arg1Of	Student,the
R21664	T27562	T27563	arg2Of	Student,'s
R21665	T27564	T27560	arg2Of	t-test,to
R21666	T27564	T27563	arg1Of	t-test,'s
R21667	T27564	T27565	arg1Of	t-test,(
R21668	T27566	T27565	arg2Of	P ,(
R21669	T27566	T27567	arg1Of	P ,<
R21670	T27567	T27568	arg1Of	<, 0.05
R21671	T27569	T27565	arg3Of	),(
R22703	T29104	T29105	arg1Of	Model,of
R22704	T29104	T29109	arg1Of	Model,by
R22705	T29104	T29121	arg1Of	Model,occupies
R22706	T29104	T29131	arg1Of	Model,interacts
R22707	T29104	T29167	arg1Of	Model,increasing
R22708	T29108	T29105	arg2Of	activation,of
R22709	T29108	T29106	arg1Of	activation,MIP-1α
R22710	T29108	T29107	arg1Of	activation,transcriptional
R22711	T29111	T29109	arg2Of	Tat.,by
R22712	T29111	T29110	arg1Of	Tat.,HIV-1
R22713	T29111	T29112	arg1Of	Tat.,(
R22714	T29111	T29115	arg1Of	Tat.,In
R22715	T29113	T29112	arg2Of	A,(
R22716	T29114	T29112	arg3Of	),(
R22717	T29116	T29115	arg2Of	absence,In
R22718	T29116	T29117	arg1Of	absence,of
R22719	T29116	T29119	arg1Of	absence,","
R22720	T29118	T29117	arg2Of	Tat,of
R22721	T29120	T29119	arg2Of	IκB-α,","
R22722	T29121	T29129	arg1Of	occupies,and
R22723	T29124	T29121	arg2Of	enhancer,occupies
R22724	T29124	T29122	arg1Of	enhancer,the
R22725	T29124	T29123	arg1Of	enhancer,NF-κB
R22726	T29124	T29125	arg1Of	enhancer,of
R22727	T29128	T29125	arg2Of	promoter,of
R22728	T29128	T29126	arg1Of	promoter,the
R22729	T29128	T29127	arg1Of	promoter,MIP-1α
R22730	T29129	T29135	arg1Of	and,","
R22731	T29129	T29137	arg1Of	and,as
R22732	T29129	T29166	arg1Of	and,by
R22733	T29131	T29129	arg2Of	interacts,and
R22734	T29131	T29130	arg1Of	interacts,likely
R22735	T29131	T29132	arg1Of	interacts,with
R22736	T29134	T29132	arg2Of	repressors,with
R22737	T29134	T29133	arg1Of	repressors,transcriptional
R22738	T29137	T29165	arg1Of	as,and
R22739	T29138	T29139	arg1Of	HDACs,(
R22740	T29138	T29142	arg1Of	HDACs,","
R22741	T29138	T29151	arg1Of	HDACs,activates
R22742	T29138	T29156	arg1Of	HDACs,removing
R22743	T29140	T29139	arg2Of	65,(
R22744	T29141	T29139	arg3Of	),(
R22745	T29144	T29142	arg2Of	inhibit,","
R22746	T29144	T29143	arg1Of	inhibit,to
R22747	T29148	T29147	arg2Of	B,(
R22748	T29149	T29147	arg3Of	),(
R22749	T29150	T29144	arg2Of	Tat,inhibit
R22750	T29150	T29145	arg1Of	Tat,gene
R22751	T29150	T29146	arg1Of	Tat,transcription.
R22752	T29150	T29147	arg1Of	Tat,(
R22753	T29151	T29137	arg2Of	activates,as
R22754	T29151	T29155	arg1Of	activates,by
R22755	T29154	T29151	arg2Of	expression,activates
R22756	T29154	T29152	arg1Of	expression,the
R22757	T29154	T29153	arg1Of	expression,MIP-1α
R22758	T29156	T29155	arg2Of	removing,by
R22759	T29159	T29156	arg2Of	repressor,removing
R22760	T29159	T29157	arg1Of	repressor,the
R22761	T29159	T29158	arg1Of	repressor,IκB-α
R22762	T29159	T29160	arg1Of	repressor,from
R22763	T29163	T29160	arg2Of	enhancer,from
R22764	T29163	T29161	arg1Of	enhancer,the
R22765	T29163	T29162	arg1Of	enhancer,NF-κB
R22766	T29165	T29136	arg1Of	and,such
R22767	T29165	T29164	arg1Of	and,","
R22768	T29166	T29165	arg2Of	by,and
R22769	T29167	T29166	arg2Of	increasing,by
R22770	T29169	T29167	arg2Of	binding,increasing
R22771	T29169	T29168	arg1Of	binding,the
R22772	T29169	T29170	arg1Of	binding,of
R22773	T29169	T29174	arg1Of	binding,to
R22774	T29173	T29170	arg2Of	complex,of
R22775	T29173	T29171	arg1Of	complex,p65
R22776	T29173	T29172	arg1Of	complex,NF-κB
R22777	T29177	T29174	arg2Of	enhancer,to
R22778	T29177	T29175	arg1Of	enhancer,the
R22779	T29177	T29176	arg1Of	enhancer,NF-κB
R1277	T1461	T1460	arg1Of	factors,transcription
R1278	T1461	T1462	arg1Of	factors,regulate
R1283	T1464	T1462	arg2Of	transcription,regulate
R1284	T1464	T1463	arg1Of	transcription,the
R10771	T13344	T13345	arg1Of	association,with
R21358	T27266	T27267	arg1Of	Tat,activates
R21359	T27266	T27275	arg1Of	Tat,occupying
R21361	T27267	T27274	arg1Of	activates,by
R21362	T27270	T27267	arg2Of	expression,activates
R21363	T27270	T27268	arg1Of	expression,the
R21364	T27270	T27269	arg1Of	expression,p65-dependent
R21365	T27270	T27271	arg1Of	expression,of
R21366	T27273	T27271	arg2Of	genes,of
R21367	T27273	T27272	arg1Of	genes,NF-κB-responsive
R21372	T27279	T27274	arg2Of	and,by
R5852	T6908	T6907	arg2Of	50,(
R5853	T6909	T6907	arg3Of	),(
R5854	T6910	T6911	arg1Of	Details,are
R5855	T6910	T6912	arg2Of	Details,described
R5856	T6912	T6911	arg2Of	described,are
R5857	T6912	T6913	arg1Of	described,in
R5858	T6915	T6913	arg2Of	Data,in
R5859	T6915	T6914	arg1Of	Data,Supplementary

bionlp-st-ge-2016-coref

Id	Subject	Object	Predicate	Lexical cue
T13716	26840-26853	Antecedent	denotes	Tat R(49–57)A
T13717	27088-27099	Anaphor	denotes	the mutants
T13718	27100-27115	Antecedent	denotes	Tat T,N(23,24)A
T13719	27117-27130	Antecedent	denotes	Tat K(50,51)A
T13720	27135-27148	Antecedent	denotes	Tat R(49–57)A
T13721	27351-27367	Anaphor	denotes	such interaction
T13722	27236-27246	Antecedent	denotes	associated
T20956	40941-40958	Anaphor	denotes	the viral protein
T20957	40872-40875	Antecedent	denotes	Tat
T20958	40971-40976	Anaphor	denotes	which
T20959	40926-40937	Antecedent	denotes	association
T20960	41439-41444	Anaphor	denotes	which
T20961	41417-41428	Antecedent	denotes	association
T20962	43300-43302	Anaphor	denotes	it
T20963	43214-43219	Antecedent	denotes	IκB-α
T20964	44878-44890	Anaphor	denotes	the cytokine
T20965	44848-44853	Antecedent	denotes	TNF-α
T20966	44858-44862	Antecedent	denotes	IL-6
T20967	44942-44959	Anaphor	denotes	the 5′ transcript
T20968	44878-44890	Antecedent	denotes	the cytokine
T9360	15690-15692	Anaphor	denotes	it
T9361	15538-15548	Antecedent	denotes	expression
T9362	17677-17681	Anaphor	denotes	they
T9363	17579-17586	Antecedent	denotes	binding
T9364	17599-17602	Antecedent	denotes	p65
T9365	19438-19442	Anaphor	denotes	they
T9366	19366-19369	Antecedent	denotes	p65
T9367	19571-19574	Anaphor	denotes	its
T9368	19505-19508	Antecedent	denotes	Tat
T13713	26793-26804	Anaphor	denotes	the mutants
T13714	26805-26820	Antecedent	denotes	Tat T,N(23,24)A
T13715	26822-26835	Antecedent	denotes	Tat K(50,51)A
T26503	27414-27417	Anaphor	denotes	its
T26504	27399-27402	Antecedent	denotes	p65
T11963	22553-22566	Anaphor	denotes	the repressor
T11964	22516-22521	Antecedent	denotes	IκB-α
T11965	26428-26441	Anaphor	denotes	the repressor
T11966	26408-26413	Antecedent	denotes	IκB-α
T356	525-538	Anaphor	denotes	the repressor
T357	501-506	Antecedent	denotes	IκB-α
T18169	35804-35806	Anaphor	denotes	it
T18170	35729-35739	Antecedent	denotes	expression
T18171	39076-39082	Anaphor	denotes	effect
T18172	38993-39002	Antecedent	denotes	activated
T2231	4416-4449	Anaphor	denotes	transacting factors and cofactors
T2232	4459-4462	Antecedent	denotes	Sp1
T2233	4469-4474	Antecedent	denotes	TFIID
T2234	4481-4486	Antecedent	denotes	E2F-4
T2235	4493-4499	Antecedent	denotes	C/EBPβ
T2236	4506-4515	Antecedent	denotes	cyclin T1
T2237	4516-4520	Antecedent	denotes	CDK9
T2238	4533-4563	Anaphor	denotes	the histone acetyltransferases
T2239	4564-4568	Antecedent	denotes	p300
T2240	4569-4572	Antecedent	denotes	CBP
T2241	4577-4582	Antecedent	denotes	P/CAF
T2242	5603-5608	Anaphor	denotes	which
T2243	5571-5582	Antecedent	denotes	interaction
T15820	30671-30682	Anaphor	denotes	the mutants
T15821	30683-30699	Antecedent	denotes	Tat C(22,25,27)A
T15822	30704-30717	Antecedent	denotes	Tat R(49–57)A
T15823	31346-31363	Anaphor	denotes	the viral protein
T15824	31298-31301	Antecedent	denotes	Tat
T15825	32986-32991	Anaphor	denotes	which
T15826	32978-32984	Antecedent	denotes	MIP-1α
T15827	34205-34207	Anaphor	denotes	it
T15828	34128-34131	Antecedent	denotes	Tat
T15829	34553-34555	Anaphor	denotes	it
T15830	34456-34459	Antecedent	denotes	Tat
T15831	34728-34735	Anaphor	denotes	binding
T15832	34633-34638	Antecedent	denotes	bound
R16926	T20960	T20961	boundBy	which,association
R16927	T20962	T20963	boundBy	it,IκB-α
R16928	T20964	T20965	boundBy	the cytokine,TNF-α
R16929	T20964	T20966	boundBy	the cytokine,IL-6
R16930	T20967	T20964	boundBy	the 5′ transcript,the cytokine
R7867	T9360	T9361	boundBy	it,expression
R7868	T9362	T9363	boundBy	they,binding
R7869	T9362	T9364	boundBy	they,p65
R7870	T9365	T9366	boundBy	they,p65
R7871	T9367	T9368	boundBy	its,Tat
R11143	T13713	T13714	boundBy	the mutants,"Tat T,N(23,24)A"
R11144	T13713	T13715	boundBy	the mutants,"Tat K(50,51)A"
R11145	T13713	T13716	boundBy	the mutants,Tat R(49–57)A
R11146	T13717	T13718	boundBy	the mutants,"Tat T,N(23,24)A"
R11147	T13717	T13719	boundBy	the mutants,"Tat K(50,51)A"
R11148	T13717	T13720	boundBy	the mutants,Tat R(49–57)A
R11149	T13721	T13722	boundBy	such interaction,associated
R16924	T20956	T20957	boundBy	the viral protein,Tat
R16925	T20958	T20959	boundBy	which,association
R20924	T26503	T26504	boundBy	its,p65
R685	T356	T357	boundBy	the repressor,IκB-α
R9815	T11963	T11964	boundBy	the repressor,IκB-α
R9816	T11965	T11966	boundBy	the repressor,IκB-α
R2022	T2231	T2232	boundBy	transacting factors and cofactors,Sp1
R2023	T2231	T2233	boundBy	transacting factors and cofactors,TFIID
R2024	T2231	T2234	boundBy	transacting factors and cofactors,E2F-4
R2025	T2231	T2235	boundBy	transacting factors and cofactors,C/EBPβ
R2026	T2231	T2236	boundBy	transacting factors and cofactors,cyclin T1
R2027	T2231	T2237	boundBy	transacting factors and cofactors,CDK9
R2030	T2238	T2239	boundBy	the histone acetyltransferases,p300
R2031	T2238	T2240	boundBy	the histone acetyltransferases,CBP
R2032	T2238	T2241	boundBy	the histone acetyltransferases,P/CAF
R2034	T2242	T2243	boundBy	which,interaction
R12781	T15820	T15821	boundBy	the mutants,"Tat C(22,25,27)A"
R12782	T15820	T15822	boundBy	the mutants,Tat R(49–57)A
R12783	T15823	T15824	boundBy	the viral protein,Tat
R12784	T15825	T15826	boundBy	which,MIP-1α
R12785	T15827	T15828	boundBy	it,Tat
R12786	T15829	T15830	boundBy	it,Tat
R12787	T15831	T15832	boundBy	binding,bound
R14635	T18169	T18170	boundBy	it,expression
R14636	T18171	T18172	boundBy	effect,activated

bionlp-st-ge-2016-spacy-parsed

Id	Subject	Object	Predicate	Lexical cue
T402	0-5	JJ	denotes	Human
T403	6-22	NN	denotes	immunodeficiency
T404	23-30	JJ	denotes	virus-1
T405	31-34	NNP	denotes	Tat
T406	35-44	VBZ	denotes	activates
T407	45-50	NNP	denotes	NF-κB
T408	51-54	IN	denotes	via
T409	55-63	JJ	denotes	physical
T410	64-75	NN	denotes	interaction
T411	76-80	IN	denotes	with
T412	81-86	NNP	denotes	IκB-α
T413	87-90	CC	denotes	and
T414	91-94	CD	denotes	p65
T415	106-113	JJ	denotes	Nuclear
T416	114-120	NN	denotes	factor
T417	121-122	-LRB-	denotes	(
T418	122-124	NNP	denotes	NF
T419	124-125	-RRB-	denotes	)
T420	125-126	:	denotes	-
T421	126-128	NN	denotes	κB
T422	129-131	VBZ	denotes	is
T423	132-133	DT	denotes	a
T424	134-140	NN	denotes	master
T425	141-150	NN	denotes	regulator
T426	151-153	IN	denotes	of
T427	154-170	JJ	denotes	pro-inflammatory
T428	171-176	NNS	denotes	genes
T429	177-180	CC	denotes	and
T430	181-183	VBZ	denotes	is
T431	184-195	VBN	denotes	upregulated
T432	196-198	IN	denotes	in
T433	199-204	JJ	denotes	human
T434	205-221	NN	denotes	immunodeficiency
T435	222-227	NN	denotes	virus
T436	228-229	CD	denotes	1
T437	230-231	-LRB-	denotes	(
T438	231-236	NN	denotes	HIV-1
T439	236-237	-RRB-	denotes	)
T440	238-247	NN	denotes	infection
T441	247-248	.	denotes	.
T442	249-259	NNPS	denotes	Mechanisms
T443	260-270	VBG	denotes	underlying
T444	271-274	DT	denotes	the
T445	275-280	JJ	denotes	NF-κB
T446	281-293	NN	denotes	deregulation
T447	294-296	IN	denotes	by
T448	297-302	NNP	denotes	HIV-1
T449	303-306	VBP	denotes	are
T450	307-315	JJ	denotes	relevant
T451	316-319	IN	denotes	for
T452	320-326	JJ	denotes	immune
T453	327-338	NN	denotes	dysfunction
T454	339-341	IN	denotes	in
T455	342-346	NNP	denotes	AIDS
T456	346-347	.	denotes	.
T457	348-350	PRP	denotes	We
T458	351-357	VBP	denotes	report
T459	358-362	IN	denotes	that
T460	363-365	IN	denotes	in
T461	366-372	JJ	denotes	single
T462	373-378	NN	denotes	round
T463	379-384	NN	denotes	HIV-1
T464	385-394	NN	denotes	infection
T465	394-395	,	denotes	,
T466	396-398	CC	denotes	or
T467	399-411	JJ	denotes	single-pulse
T468	412-415	NNP	denotes	PMA
T469	416-427	NN	denotes	stimulation
T470	427-428	,	denotes	,
T471	429-432	DT	denotes	the
T472	433-438	NNP	denotes	HIV-1
T473	439-442	NNP	denotes	Tat
T474	443-457	NN	denotes	transactivator
T475	458-467	VBD	denotes	activated
T476	468-473	JJ	denotes	NF-κB
T477	474-476	IN	denotes	by
T478	477-486	VBG	denotes	hijacking
T479	487-490	DT	denotes	the
T480	491-500	NN	denotes	inhibitor
T481	501-506	NN	denotes	IκB-α
T482	507-510	CC	denotes	and
T483	511-513	IN	denotes	by
T484	514-524	VBG	denotes	preventing
T485	525-528	DT	denotes	the
T486	529-538	NN	denotes	repressor
T487	539-546	JJ	denotes	binding
T488	547-549	TO	denotes	to
T489	550-553	DT	denotes	the
T490	554-559	JJ	denotes	NF-κB
T491	560-567	NN	denotes	complex
T492	567-568	.	denotes	.
T493	569-577	RB	denotes	Moreover
T494	577-578	,	denotes	,
T495	579-582	NNP	denotes	Tat
T496	583-593	VBN	denotes	associated
T497	594-598	IN	denotes	with
T498	599-602	DT	denotes	the
T499	603-606	CD	denotes	p65
T500	607-614	NN	denotes	subunit
T501	615-617	IN	denotes	of
T502	618-623	NNP	denotes	NF-κB
T503	624-627	CC	denotes	and
T504	628-637	VBD	denotes	increased
T505	638-641	DT	denotes	the
T506	642-645	CD	denotes	p65
T507	646-657	JJ	denotes	DNA-binding
T508	658-666	NN	denotes	affinity
T509	667-670	CC	denotes	and
T510	671-686	JJ	denotes	transcriptional
T511	687-695	NN	denotes	activity
T512	695-696	.	denotes	.
T513	697-700	DT	denotes	The
T514	701-709	NN	denotes	arginine
T515	709-710	:	denotes	-
T516	711-714	CC	denotes	and
T517	715-728	JJ	denotes	cysteine-rich
T518	729-736	NNS	denotes	domains
T519	737-739	IN	denotes	of
T520	740-743	NNP	denotes	Tat
T521	744-748	VBD	denotes	were
T522	749-757	VBN	denotes	required
T523	758-761	IN	denotes	for
T524	762-767	NNP	denotes	IκB-α
T525	768-771	CC	denotes	and
T526	772-775	CD	denotes	p65
T527	776-787	NN	denotes	association
T528	787-788	,	denotes	,
T529	789-801	RB	denotes	respectively
T530	801-802	,	denotes	,
T531	803-806	CC	denotes	and
T532	807-810	IN	denotes	for
T533	811-821	VBG	denotes	sustaining
T534	822-825	DT	denotes	the
T535	826-831	NN	denotes	NF-κB
T536	832-840	NN	denotes	activity
T537	840-841	.	denotes	.
T538	842-847	IN	denotes	Among
T539	848-850	DT	denotes	an
T540	851-856	NN	denotes	array
T541	857-859	IN	denotes	of
T542	860-876	JJ	denotes	NF-κB-responsive
T543	877-882	NNS	denotes	genes
T544	882-883	,	denotes	,
T545	884-887	NNP	denotes	Tat
T546	888-894	RB	denotes	mostly
T547	895-904	VBD	denotes	activated
T548	905-908	DT	denotes	the
T549	909-915	JJ	denotes	MIP-1α
T550	916-926	NN	denotes	expression
T551	927-929	IN	denotes	in
T552	930-931	DT	denotes	a
T553	932-945	JJ	denotes	p65-dependent
T554	946-952	NN	denotes	manner
T555	952-953	,	denotes	,
T556	954-957	CC	denotes	and
T557	958-963	VBN	denotes	bound
T558	964-966	TO	denotes	to
T559	967-970	DT	denotes	the
T560	971-977	NNP	denotes	MIP-1α
T561	978-983	NNP	denotes	NF-κB
T562	984-992	NN	denotes	enhancer
T563	993-997	RB	denotes	thus
T564	998-1007	VBG	denotes	promoting
T565	1008-1011	DT	denotes	the
T566	1012-1023	NN	denotes	recruitment
T567	1024-1026	IN	denotes	of
T568	1027-1030	NNS	denotes	p65
T569	1031-1035	IN	denotes	with
T570	1036-1048	NN	denotes	displacement
T571	1049-1051	IN	denotes	of
T572	1052-1057	NNP	denotes	IκB-α
T573	1057-1058	:	denotes	;
T574	1059-1066	JJ	denotes	similar
T575	1067-1075	NNS	denotes	findings
T576	1076-1080	VBD	denotes	were
T577	1081-1089	VBN	denotes	obtained
T578	1090-1093	IN	denotes	for
T579	1094-1097	DT	denotes	the
T580	1098-1114	JJ	denotes	NF-κB-responsive
T581	1115-1120	NNS	denotes	genes
T582	1121-1125	NNP	denotes	CSF3
T583	1125-1126	,	denotes	,
T584	1127-1130	NNP	denotes	LTA
T585	1130-1131	,	denotes	,
T586	1132-1138	NNP	denotes	NFKBIA
T587	1139-1142	CC	denotes	and
T588	1143-1147	NNP	denotes	TLR2
T589	1147-1148	.	denotes	.
T590	1149-1152	PRP$	denotes	Our
T591	1153-1160	NNS	denotes	results
T592	1161-1168	VBP	denotes	support
T593	1169-1170	DT	denotes	a
T594	1171-1176	NN	denotes	novel
T595	1177-1186	NN	denotes	mechanism
T596	1187-1189	IN	denotes	of
T597	1190-1195	JJ	denotes	NF-κB
T598	1196-1206	NN	denotes	activation
T599	1207-1210	IN	denotes	via
T600	1211-1219	JJ	denotes	physical
T601	1220-1231	NN	denotes	interaction
T602	1232-1234	IN	denotes	of
T603	1235-1238	NNP	denotes	Tat
T604	1239-1243	IN	denotes	with
T605	1244-1249	NNP	denotes	IκB-α
T606	1250-1253	CC	denotes	and
T607	1254-1257	CD	denotes	p65
T608	1257-1258	,	denotes	,
T609	1259-1262	CC	denotes	and
T610	1263-1266	MD	denotes	may
T611	1267-1277	VB	denotes	contribute
T612	1278-1280	TO	denotes	to
T613	1281-1288	JJ	denotes	further
T614	1289-1297	NNS	denotes	insights
T615	1298-1302	IN	denotes	into
T616	1303-1306	DT	denotes	the
T617	1307-1319	NN	denotes	deregulation
T618	1320-1322	IN	denotes	of
T619	1323-1326	DT	denotes	the
T620	1327-1339	JJ	denotes	inflammatory
T621	1340-1348	NN	denotes	response
T622	1349-1351	IN	denotes	by
T623	1352-1357	NNP	denotes	HIV-1
T624	1357-1358	.	denotes	.
T2329	1373-1380	NNP	denotes	Nuclear
T2330	1381-1387	NN	denotes	factor
T2331	1388-1389	-LRB-	denotes	(
T2332	1389-1391	NNP	denotes	NF
T2333	1391-1392	-RRB-	denotes	)
T2334	1392-1393	:	denotes	-
T2335	1393-1395	NN	denotes	κB
T2336	1396-1409	NN	denotes	transcription
T2337	1410-1417	NNS	denotes	factors
T2338	1418-1426	VB	denotes	regulate
T2339	1427-1430	DT	denotes	the
T2340	1431-1444	NN	denotes	transcription
T2341	1445-1447	IN	denotes	of
T2342	1448-1453	NNS	denotes	genes
T2343	1454-1458	WDT	denotes	that
T2344	1459-1462	VBP	denotes	are
T2345	1463-1471	VBN	denotes	involved
T2346	1472-1474	IN	denotes	in
T2347	1475-1478	DT	denotes	the
T2348	1479-1485	JJ	denotes	immune
T2349	1486-1489	CC	denotes	and
T2350	1490-1502	JJ	denotes	inflammatory
T2351	1503-1511	NN	denotes	response
T2352	1512-1513	-LRB-	denotes	(
T2353	1513-1514	CD	denotes	1
T2354	1514-1515	-RRB-	denotes	)
T2355	1515-1516	.	denotes	.
T2356	1517-1520	DT	denotes	The
T2357	1521-1526	NNP	denotes	NF-κB
T2358	1527-1533	NN	denotes	family
T2359	1534-1542	VBZ	denotes	includes
T2360	1543-1551	NNS	denotes	RelA/p65
T2361	1551-1552	,	denotes	,
T2362	1553-1558	NNP	denotes	c-Rel
T2363	1558-1559	,	denotes	,
T2364	1560-1564	NNP	denotes	RelB
T2365	1564-1565	,	denotes	,
T2366	1566-1569	CD	denotes	p50
T2367	1570-1573	CC	denotes	and
T2368	1574-1577	CD	denotes	p52
T2369	1578-1582	WDT	denotes	that
T2370	1583-1588	VBP	denotes	share
T2371	1589-1590	DT	denotes	a
T2372	1591-1597	RB	denotes	highly
T2373	1598-1607	VBN	denotes	conserved
T2374	1608-1617	JJ	denotes	300-amino
T2375	1618-1622	NN	denotes	acid
T2376	1623-1626	NNP	denotes	Rel
T2377	1627-1635	NN	denotes	homology
T2378	1636-1642	NN	denotes	domain
T2379	1643-1644	-LRB-	denotes	(
T2380	1644-1647	NNP	denotes	RHD
T2381	1647-1648	-RRB-	denotes	)
T2382	1649-1652	IN	denotes	for
T2383	1653-1657	NN	denotes	homo
T2384	1657-1658	:	denotes	-
T2385	1659-1661	CC	denotes	or
T2386	1662-1681	NN	denotes	hetero-dimerization
T2387	1682-1685	CC	denotes	and
T2388	1686-1697	JJ	denotes	DNA-binding
T2389	1697-1698	.	denotes	.
T2390	1699-1702	DT	denotes	The
T2391	1703-1718	JJ	denotes	transcriptional
T2392	1719-1727	NN	denotes	activity
T2393	1728-1730	IN	denotes	of
T2394	1731-1734	DT	denotes	the
T2395	1735-1740	JJ	denotes	NF-κB
T2396	1741-1748	JJ	denotes	complex
T2397	1749-1756	VBZ	denotes	depends
T2398	1757-1759	IN	denotes	on
T2399	1760-1765	NN	denotes	dimer
T2400	1766-1777	NN	denotes	composition
T2401	1778-1783	IN	denotes	since
T2402	1784-1794	JJ	denotes	C-terminal
T2403	1795-1804	JJ	denotes	unrelated
T2404	1805-1820	JJ	denotes	transcriptional
T2405	1821-1831	NN	denotes	activation
T2406	1832-1839	NNS	denotes	domains
T2407	1840-1843	VBP	denotes	are
T2408	1844-1851	JJ	denotes	present
T2409	1852-1863	RB	denotes	exclusively
T2410	1864-1866	IN	denotes	in
T2411	1867-1870	CD	denotes	p65
T2412	1870-1871	,	denotes	,
T2413	1872-1876	NNP	denotes	RelB
T2414	1877-1880	CC	denotes	and
T2415	1881-1886	NNP	denotes	c-Rel
T2416	1887-1888	-LRB-	denotes	(
T2417	1888-1889	LS	denotes	2
T2418	1889-1890	-RRB-	denotes	)
T2419	1890-1891	.	denotes	.
T2420	1892-1902	NNS	denotes	Inhibitors
T2421	1903-1905	IN	denotes	of
T2422	1906-1911	NNP	denotes	NF-κB
T2423	1912-1913	-LRB-	denotes	(
T2424	1913-1916	NNP	denotes	IκB
T2425	1916-1917	-RRB-	denotes	)
T2426	1918-1927	NN	denotes	associate
T2427	1928-1932	IN	denotes	with
T2428	1933-1936	DT	denotes	the
T2429	1937-1942	NNP	denotes	NF-κB
T2430	1943-1950	NN	denotes	complex
T2431	1951-1954	CC	denotes	and
T2432	1955-1964	VB	denotes	interfere
T2433	1965-1969	IN	denotes	with
T2434	1970-1973	PRP$	denotes	its
T2435	1974-1981	JJ	denotes	binding
T2436	1982-1984	TO	denotes	to
T2437	1985-1988	NNP	denotes	DNA
T2438	1989-1990	-LRB-	denotes	(
T2439	1990-1991	LS	denotes	3
T2440	1991-1992	-RRB-	denotes	)
T2441	1992-1993	.	denotes	.
T2442	1994-1996	IN	denotes	In
T2443	1997-2000	DT	denotes	the
T2444	2001-2010	JJ	denotes	canonical
T2445	2011-2018	NN	denotes	pathway
T2446	2019-2021	IN	denotes	of
T2447	2022-2027	JJ	denotes	NF-κB
T2448	2028-2038	NN	denotes	activation
T2449	2038-2039	,	denotes	,
T2450	2040-2043	DT	denotes	the
T2451	2044-2053	VBN	denotes	activated
T2452	2054-2057	NNP	denotes	IκB
T2453	2058-2064	NN	denotes	kinase
T2454	2065-2066	-LRB-	denotes	(
T2455	2066-2069	NNP	denotes	IKK
T2456	2069-2070	-RRB-	denotes	)
T2457	2071-2085	VBZ	denotes	phosphorylates
T2458	2086-2089	NNP	denotes	IκB
T2459	2090-2092	IN	denotes	at
T2460	2093-2101	JJ	denotes	specific
T2461	2102-2108	NN	denotes	serine
T2462	2109-2117	NNS	denotes	residues
T2463	2118-2122	WDT	denotes	that
T2464	2123-2129	VBP	denotes	target
T2465	2130-2133	DT	denotes	the
T2466	2134-2141	NN	denotes	protein
T2467	2142-2144	TO	denotes	to
T2468	2145-2159	NN	denotes	ubiquitination
T2469	2160-2163	CC	denotes	and
T2470	2164-2175	JJ	denotes	proteasomal
T2471	2176-2187	NN	denotes	degradation
T2472	2187-2188	,	denotes	,
T2473	2189-2194	WDT	denotes	which
T2474	2195-2203	VBZ	denotes	releases
T2475	2204-2207	DT	denotes	the
T2476	2208-2218	JJ	denotes	functional
T2477	2219-2224	NN	denotes	NF-κB
T2478	2225-2232	NN	denotes	complex
T2479	2233-2235	IN	denotes	in
T2480	2236-2239	DT	denotes	the
T2481	2240-2247	NN	denotes	nucleus
T2482	2247-2248	.	denotes	.
T2483	2249-2254	NNP	denotes	IκB-α
T2484	2254-2255	,	denotes	,
T2485	2256-2259	DT	denotes	the
T2486	2260-2264	RBS	denotes	most
T2487	2265-2273	JJ	denotes	abundant
T2488	2274-2283	NN	denotes	inhibitor
T2489	2284-2286	IN	denotes	of
T2490	2287-2292	NNP	denotes	NF-κB
T2491	2293-2294	-LRB-	denotes	(
T2492	2294-2295	LS	denotes	4
T2493	2295-2296	-RRB-	denotes	)
T2494	2296-2297	,	denotes	,
T2495	2298-2300	VBZ	denotes	is
T2496	2301-2315	VBN	denotes	phosphorylated
T2497	2316-2318	IN	denotes	by
T2498	2319-2322	NNP	denotes	IKK
T2499	2323-2325	IN	denotes	at
T2500	2326-2331	NNP	denotes	Ser32
T2501	2332-2335	CC	denotes	and
T2502	2336-2341	NNP	denotes	Ser36
T2503	2342-2343	-LRB-	denotes	(
T2504	2343-2344	CD	denotes	5
T2505	2344-2345	-RRB-	denotes	)
T2506	2345-2346	,	denotes	,
T2507	2347-2350	CC	denotes	and
T2508	2351-2363	RB	denotes	subsequently
T2509	2364-2377	VBD	denotes	ubiquitylated
T2510	2378-2380	IN	denotes	at
T2511	2381-2386	NNP	denotes	Lys21
T2512	2387-2390	CC	denotes	and
T2513	2391-2396	NNP	denotes	Lys22
T2514	2397-2399	TO	denotes	to
T2515	2400-2402	VB	denotes	be
T2516	2403-2411	JJ	denotes	degraded
T2517	2412-2414	IN	denotes	by
T2518	2415-2418	DT	denotes	the
T2519	2419-2422	JJ	denotes	26S
T2520	2423-2433	JJ	denotes	proteasome
T2521	2434-2435	-LRB-	denotes	(
T2522	2435-2436	CD	denotes	6
T2523	2436-2437	-RRB-	denotes	)
T2524	2437-2438	.	denotes	.
T2525	2439-2442	DT	denotes	The
T2526	2443-2448	JJ	denotes	NF-κB
T2527	2449-2457	NN	denotes	activity
T2528	2458-2460	VBZ	denotes	is
T2529	2461-2469	VBN	denotes	enhanced
T2530	2470-2472	IN	denotes	by
T2531	2473-2488	NN	denotes	phosphorylation
T2532	2489-2491	IN	denotes	of
T2533	2492-2495	CD	denotes	p65
T2534	2496-2498	IN	denotes	at
T2535	2499-2505	CD	denotes	Ser276
T2536	2506-2508	IN	denotes	by
T2537	2509-2512	NNP	denotes	PKA
T2538	2513-2516	CC	denotes	and
T2539	2517-2521	NNP	denotes	MSK1
T2540	2522-2523	-LRB-	denotes	(
T2541	2523-2526	CD	denotes	7,8
T2542	2526-2527	-RRB-	denotes	)
T2543	2527-2528	,	denotes	,
T2544	2529-2535	NNP	denotes	Ser311
T2545	2536-2538	IN	denotes	by
T2546	2539-2543	NNP	denotes	PKCζ
T2547	2544-2545	-LRB-	denotes	(
T2548	2545-2546	CD	denotes	9
T2549	2546-2547	-RRB-	denotes	)
T2550	2548-2551	CC	denotes	and
T2551	2552-2558	NNP	denotes	Ser536
T2552	2559-2561	IN	denotes	by
T2553	2562-2566	NNP	denotes	IKKα
T2554	2567-2568	-LRB-	denotes	(
T2555	2568-2573	CD	denotes	10,11
T2556	2573-2574	-RRB-	denotes	)
T2557	2574-2575	.	denotes	.
T2558	2576-2587	NNP	denotes	Acetylation
T2559	2588-2590	IN	denotes	of
T2560	2591-2594	CD	denotes	p65
T2561	2595-2597	IN	denotes	at
T2562	2598-2604	NNP	denotes	Lys218
T2563	2605-2608	CC	denotes	and
T2564	2609-2615	NNP	denotes	Lys221
T2565	2616-2625	VBZ	denotes	increases
T2566	2626-2629	DT	denotes	the
T2567	2630-2633	NNP	denotes	DNA
T2568	2634-2641	JJ	denotes	binding
T2569	2642-2645	CC	denotes	and
T2570	2646-2653	VBZ	denotes	impairs
T2571	2654-2657	DT	denotes	the
T2572	2658-2669	NN	denotes	association
T2573	2670-2674	IN	denotes	with
T2574	2675-2680	NNP	denotes	IκB-α
T2575	2680-2681	,	denotes	,
T2576	2682-2685	CC	denotes	and
T2577	2686-2697	NN	denotes	acetylation
T2578	2698-2700	IN	denotes	at
T2579	2701-2707	NNP	denotes	Lys310
T2580	2708-2716	VBZ	denotes	enhances
T2581	2717-2720	DT	denotes	the
T2582	2721-2724	CD	denotes	p65
T2583	2725-2740	JJ	denotes	transcriptional
T2584	2741-2749	NN	denotes	activity
T2585	2750-2751	-LRB-	denotes	(
T2586	2751-2756	CD	denotes	12,13
T2587	2756-2757	-RRB-	denotes	)
T2588	2757-2758	.	denotes	.
T2589	2759-2774	NN	denotes	Post-activation
T2590	2775-2779	VB	denotes	turn
T2591	2780-2783	RB	denotes	off
T2592	2784-2786	IN	denotes	of
T2593	2787-2792	NN	denotes	NF-κB
T2594	2793-2795	VBZ	denotes	is
T2595	2796-2805	VBN	denotes	regulated
T2596	2806-2808	IN	denotes	by
T2597	2809-2817	JJ	denotes	negative
T2598	2818-2826	NN	denotes	feedback
T2599	2827-2831	NN	denotes	loop
T2600	2832-2839	IN	denotes	through
T2601	2840-2850	NNS	denotes	inhibitors
T2602	2851-2856	IN	denotes	under
T2603	2857-2860	DT	denotes	the
T2604	2861-2876	JJ	denotes	transcriptional
T2605	2877-2884	NN	denotes	control
T2606	2885-2887	IN	denotes	of
T2607	2888-2893	NNP	denotes	NF-κB
T2608	2893-2894	,	denotes	,
T2609	2895-2899	JJ	denotes	such
T2610	2900-2902	IN	denotes	as
T2611	2903-2908	JJ	denotes	IκB-α
T2612	2909-2912	CC	denotes	and
T2613	2913-2930	JJ	denotes	ubiquitin-editing
T2614	2931-2938	NN	denotes	protein
T2615	2939-2942	NNP	denotes	A20
T2616	2943-2944	-LRB-	denotes	(
T2617	2944-2946	CD	denotes	14
T2618	2947-2949	CD	denotes	20
T2619	2949-2950	-RRB-	denotes	)
T2620	2950-2951	.	denotes	.
T2621	2952-2965	NN	denotes	Deacetylation
T2622	2966-2968	IN	denotes	of
T2623	2969-2972	CD	denotes	p65
T2624	2973-2975	IN	denotes	by
T2625	2976-2983	NN	denotes	histone
T2626	2984-2997	JJ	denotes	deacetylase-3
T2627	2998-3000	CC	denotes	or
T2628	3001-3006	CD	denotes	SIRT1
T2629	3006-3007	,	denotes	,
T2630	3008-3010	CC	denotes	or
T2631	3011-3022	NN	denotes	acetylation
T2632	3023-3025	IN	denotes	of
T2633	3026-3029	NNS	denotes	p65
T2634	3030-3032	IN	denotes	at
T2635	3033-3039	NNP	denotes	Lys122
T2636	3040-3043	CC	denotes	and
T2637	3044-3050	NNP	denotes	Lys123
T2638	3051-3064	VBP	denotes	down-regulate
T2639	3065-3068	DT	denotes	the
T2640	3069-3074	NN	denotes	NF-κB
T2641	3075-3083	NN	denotes	activity
T2642	3084-3085	-LRB-	denotes	(
T2643	3085-3093	CD	denotes	12,21,22
T2644	3093-3094	-RRB-	denotes	)
T2645	3094-3095	.	denotes	.
T2646	3096-3106	JJ	denotes	Persistent
T2647	3107-3117	NN	denotes	activation
T2648	3118-3120	IN	denotes	of
T2649	3121-3126	JJ	denotes	NF-κB
T2650	3127-3133	VBZ	denotes	occurs
T2651	3134-3136	IN	denotes	in
T2652	3137-3142	JJ	denotes	human
T2653	3143-3159	NN	denotes	immunodeficiency
T2654	3160-3167	JJ	denotes	virus-1
T2655	3168-3169	-LRB-	denotes	(
T2656	3169-3174	NNP	denotes	HIV-1
T2657	3174-3175	-RRB-	denotes	)
T2658	3175-3176	:	denotes	-
T2659	3176-3184	VBN	denotes	infected
T2660	3185-3194	NNS	denotes	monocytes
T2661	3194-3195	,	denotes	,
T2662	3196-3207	NNS	denotes	macrophages
T2663	3208-3211	CC	denotes	and
T2664	3212-3221	NNS	denotes	microglia
T2665	3221-3222	,	denotes	,
T2666	3223-3226	CC	denotes	and
T2667	3227-3235	VBZ	denotes	enhances
T2668	3236-3239	DT	denotes	the
T2669	3240-3250	NN	denotes	expression
T2670	3251-3253	IN	denotes	of
T2671	3254-3270	JJ	denotes	NF-κB-responsive
T2672	3271-3276	NNS	denotes	genes
T2673	3276-3277	,	denotes	,
T2674	3278-3287	VBG	denotes	including
T2675	3288-3304	JJ	denotes	pro-inflammatory
T2676	3305-3314	NNS	denotes	cytokines
T2677	3314-3315	,	denotes	,
T2678	3316-3320	NN	denotes	cell
T2679	3321-3329	NN	denotes	adhesion
T2680	3330-3339	NNS	denotes	molecules
T2681	3340-3343	CC	denotes	and
T2682	3344-3354	NNS	denotes	chemokines
T2683	3355-3356	-LRB-	denotes	(
T2684	3356-3358	CD	denotes	23
T2685	3359-3361	CD	denotes	25
T2686	3361-3362	-RRB-	denotes	)
T2687	3362-3363	.	denotes	.
T2688	3364-3371	JJ	denotes	Chronic
T2689	3372-3384	NN	denotes	inflammation
T2690	3385-3387	VBZ	denotes	is
T2691	3388-3389	DT	denotes	a
T2692	3390-3395	JJ	denotes	major
T2693	3396-3401	NN	denotes	cause
T2694	3402-3404	IN	denotes	of
T2695	3405-3411	JJ	denotes	immune
T2696	3412-3415	CC	denotes	and
T2697	3416-3422	JJ	denotes	neuron
T2698	3423-3434	NN	denotes	dysfunction
T2699	3435-3437	IN	denotes	in
T2700	3438-3442	NNP	denotes	AIDS
T2701	3443-3444	-LRB-	denotes	(
T2702	3444-3449	CD	denotes	26,27
T2703	3449-3450	-RRB-	denotes	)
T2704	3450-3451	.	denotes	.
T2705	3452-3464	RB	denotes	Consistently
T2706	3464-3465	,	denotes	,
T2707	3466-3475	JJ	denotes	non-human
T2708	3476-3483	NN	denotes	primate
T2709	3484-3489	NNS	denotes	hosts
T2710	3490-3493	IN	denotes	for
T2711	3494-3500	JJ	denotes	simian
T2712	3501-3517	NN	denotes	immunodeficiency
T2713	3518-3523	NN	denotes	virus
T2714	3523-3524	,	denotes	,
T2715	3525-3529	JJ	denotes	such
T2716	3530-3532	IN	denotes	as
T2717	3533-3540	JJ	denotes	African
T2718	3541-3546	JJ	denotes	green
T2719	3547-3554	NNS	denotes	monkeys
T2720	3555-3558	CC	denotes	and
T2721	3559-3564	JJ	denotes	sooty
T2722	3565-3573	NN	denotes	mangabey
T2723	3573-3574	,	denotes	,
T2724	3575-3579	VBP	denotes	lack
T2725	3580-3588	JJ	denotes	aberrant
T2726	3589-3595	JJ	denotes	immune
T2727	3596-3606	NN	denotes	activation
T2728	3607-3610	CC	denotes	and
T2729	3611-3613	VB	denotes	do
T2730	3614-3617	RB	denotes	not
T2731	3618-3625	VB	denotes	develop
T2732	3626-3630	NNP	denotes	AIDS
T2733	3631-3638	IN	denotes	despite
T2734	3639-3643	JJ	denotes	high
T2735	3644-3649	NN	denotes	virus
T2736	3650-3661	NN	denotes	replication
T2737	3662-3663	-LRB-	denotes	(
T2738	3663-3668	CD	denotes	28,29
T2739	3668-3669	-RRB-	denotes	)
T2740	3669-3670	.	denotes	.
T2741	3671-3675	RB	denotes	Thus
T2742	3675-3676	,	denotes	,
T2743	3677-3690	VBG	denotes	understanding
T2744	3691-3694	DT	denotes	the
T2745	3695-3705	NNS	denotes	mechanisms
T2746	3706-3708	IN	denotes	of
T2747	3709-3714	JJ	denotes	NF-κB
T2748	3715-3727	NN	denotes	deregulation
T2749	3728-3730	IN	denotes	by
T2750	3731-3736	NNP	denotes	HIV-1
T2751	3737-3740	MD	denotes	may
T2752	3741-3748	VB	denotes	provide
T2753	3749-3756	JJ	denotes	further
T2754	3757-3765	NNS	denotes	insights
T2755	3766-3770	IN	denotes	into
T2756	3771-3775	NNP	denotes	AIDS
T2757	3776-3788	NN	denotes	pathogenesis
T2758	3788-3789	.	denotes	.
T2759	3790-3792	IN	denotes	In
T2760	3793-3798	JJ	denotes	HIV-1
T2761	3799-3804	NN	denotes	entry
T2762	3804-3805	,	denotes	,
T2763	3806-3809	DT	denotes	the
T2764	3810-3817	JJ	denotes	binding
T2765	3818-3820	IN	denotes	of
T2766	3821-3824	DT	denotes	the
T2767	3825-3830	NNS	denotes	gp120
T2768	3831-3836	JJ	denotes	viral
T2769	3837-3845	NN	denotes	envelope
T2770	3846-3848	TO	denotes	to
T2771	3849-3852	CD	denotes	CD4
T2772	3853-3860	VBZ	denotes	induces
T2773	3861-3864	DT	denotes	the
T2774	3865-3870	JJ	denotes	NF-κB
T2775	3871-3879	NN	denotes	activity
T2776	3880-3882	IN	denotes	by
T2777	3883-3893	NN	denotes	activation
T2778	3894-3896	IN	denotes	of
T2779	3897-3900	NNP	denotes	IKK
T2780	3901-3902	-LRB-	denotes	(
T2781	3902-3904	CD	denotes	30
T2782	3904-3905	-RRB-	denotes	)
T2783	3906-3909	CC	denotes	and
T2784	3910-3920	NN	denotes	procaspase
T2785	3921-3922	CD	denotes	8
T2786	3923-3924	-LRB-	denotes	(
T2787	3924-3926	CD	denotes	31
T2788	3926-3927	-RRB-	denotes	)
T2789	3927-3928	.	denotes	.
T2790	3929-3938	VBG	denotes	Following
T2791	3939-3944	JJ	denotes	viral
T2792	3945-3956	NN	denotes	integration
T2793	3956-3957	,	denotes	,
T2794	3958-3961	DT	denotes	the
T2795	3962-3967	JJ	denotes	early
T2796	3968-3975	JJ	denotes	encoded
T2797	3976-3981	NNP	denotes	HIV-1
T2798	3982-3985	NNP	denotes	Tat
T2799	3986-3993	NN	denotes	protein
T2800	3994-4003	NNS	denotes	interacts
T2801	4004-4008	IN	denotes	with
T2802	4009-4012	DT	denotes	the
T2803	4013-4018	NNP	denotes	HIV-1
T2804	4019-4022	NNP	denotes	RNA
T2805	4023-4026	CC	denotes	and
T2806	4027-4031	NN	denotes	host
T2807	4032-4036	NN	denotes	cell
T2808	4037-4044	NNS	denotes	factors
T2809	4045-4047	TO	denotes	to
T2810	4048-4055	VB	denotes	sustain
T2811	4056-4059	DT	denotes	the
T2812	4060-4065	JJ	denotes	viral
T2813	4066-4077	NN	denotes	replication
T2814	4077-4078	.	denotes	.
T2815	4079-4082	NNP	denotes	Tat
T2816	4083-4088	VBZ	denotes	binds
T2817	4089-4091	TO	denotes	to
T2818	4092-4095	NNP	denotes	RNA
T2819	4096-4105	JJ	denotes	stem-loop
T2820	4106-4116	NNS	denotes	structures
T2821	4117-4126	VBN	denotes	generated
T2822	4127-4129	IN	denotes	by
T2823	4130-4133	DT	denotes	the
T2824	4134-4135	CD	denotes	5
T2825	4135-4136	NN	denotes	′
T2826	4137-4140	NN	denotes	end
T2827	4141-4143	IN	denotes	of
T2828	4144-4150	NN	denotes	target
T2829	4151-4162	NNS	denotes	transcripts
T2830	4162-4163	,	denotes	,
T2831	4164-4173	VBG	denotes	including
T2832	4174-4177	DT	denotes	the
T2833	4178-4183	NNP	denotes	HIV-1
T2834	4184-4210	JJ	denotes	transactivation-responsive
T2835	4211-4218	NN	denotes	element
T2836	4219-4220	-LRB-	denotes	(
T2837	4220-4223	NNP	denotes	TAR
T2838	4223-4224	-RRB-	denotes	)
T2839	4225-4226	-LRB-	denotes	(
T2840	4226-4228	CD	denotes	32
T2841	4228-4229	-RRB-	denotes	)
T2842	4229-4230	,	denotes	,
T2843	4231-4236	NN	denotes	tumor
T2844	4237-4245	NN	denotes	necrosis
T2845	4246-4252	NN	denotes	factor
T2846	4253-4254	NN	denotes	β
T2847	4255-4256	-LRB-	denotes	(
T2848	4256-4260	NNP	denotes	TNFβ
T2849	4260-4261	-RRB-	denotes	)
T2850	4262-4263	-LRB-	denotes	(
T2851	4263-4265	CD	denotes	33
T2852	4265-4266	-RRB-	denotes	)
T2853	4267-4270	CC	denotes	and
T2854	4271-4284	JJ	denotes	interleukin-6
T2855	4285-4286	-LRB-	denotes	(
T2856	4286-4290	NN	denotes	IL-6
T2857	4290-4291	-RRB-	denotes	)
T2858	4292-4293	-LRB-	denotes	(
T2859	4293-4295	CD	denotes	34
T2860	4295-4296	-RRB-	denotes	)
T2861	4297-4299	TO	denotes	to
T2862	4300-4308	VB	denotes	activate
T2863	4309-4313	NN	denotes	gene
T2864	4314-4327	NN	denotes	transcription
T2865	4327-4328	.	denotes	.
T2866	4329-4335	RB	denotes	Indeed
T2867	4335-4336	,	denotes	,
T2868	4337-4340	NNP	denotes	Tat
T2869	4341-4349	VBZ	denotes	promotes
T2870	4350-4353	DT	denotes	the
T2871	4354-4369	JJ	denotes	transcriptional
T2872	4370-4380	NN	denotes	initiation
T2873	4381-4384	CC	denotes	and
T2874	4385-4395	NN	denotes	elongation
T2875	4396-4398	IN	denotes	by
T2876	4399-4410	VBG	denotes	interacting
T2877	4411-4415	IN	denotes	with
T2878	4416-4427	VBG	denotes	transacting
T2879	4428-4435	NNS	denotes	factors
T2880	4436-4439	CC	denotes	and
T2881	4440-4449	NNS	denotes	cofactors
T2882	4449-4450	,	denotes	,
T2883	4451-4455	JJ	denotes	such
T2884	4456-4458	IN	denotes	as
T2885	4459-4462	NNP	denotes	Sp1
T2886	4463-4464	-LRB-	denotes	(
T2887	4464-4466	CD	denotes	35
T2888	4466-4467	-RRB-	denotes	)
T2889	4467-4468	,	denotes	,
T2890	4469-4474	NNP	denotes	TFIID
T2891	4475-4476	-LRB-	denotes	(
T2892	4476-4478	CD	denotes	36
T2893	4478-4479	-RRB-	denotes	)
T2894	4479-4480	,	denotes	,
T2895	4481-4486	NNP	denotes	E2F-4
T2896	4487-4488	-LRB-	denotes	(
T2897	4488-4490	CD	denotes	37
T2898	4490-4491	-RRB-	denotes	)
T2899	4491-4492	,	denotes	,
T2900	4493-4498	NNP	denotes	C/EBP
T2901	4498-4499	NNP	denotes	β
T2902	4500-4501	-LRB-	denotes	(
T2903	4501-4503	CD	denotes	38
T2904	4503-4504	-RRB-	denotes	)
T2905	4504-4505	,	denotes	,
T2906	4506-4512	NN	denotes	cyclin
T2907	4513-4520	CD	denotes	T1/CDK9
T2908	4521-4522	-LRB-	denotes	(
T2909	4522-4527	CD	denotes	39,40
T2910	4527-4528	-RRB-	denotes	)
T2911	4529-4532	CC	denotes	and
T2912	4533-4536	DT	denotes	the
T2913	4537-4544	NN	denotes	histone
T2914	4545-4563	VBZ	denotes	acetyltransferases
T2915	4564-4572	NNP	denotes	p300/CBP
T2916	4573-4576	CC	denotes	and
T2917	4577-4582	NNP	denotes	P/CAF
T2918	4583-4584	-LRB-	denotes	(
T2919	4584-4586	CD	denotes	41
T2920	4587-4589	CD	denotes	43
T2921	4589-4590	-RRB-	denotes	)
T2922	4590-4591	.	denotes	.
T2923	4592-4596	WRB	denotes	When
T2924	4597-4605	VBN	denotes	released
T2925	4606-4610	IN	denotes	from
T2926	4611-4625	JJ	denotes	HIV-1-infected
T2927	4626-4631	NNS	denotes	cells
T2928	4631-4632	,	denotes	,
T2929	4633-4636	NNP	denotes	Tat
T2930	4637-4648	VBZ	denotes	deregulates
T2931	4649-4652	DT	denotes	the
T2932	4653-4657	NN	denotes	cell
T2933	4658-4667	VBG	denotes	signaling
T2934	4668-4670	IN	denotes	by
T2935	4671-4678	JJ	denotes	binding
T2936	4679-4681	TO	denotes	to
T2937	4682-4686	NN	denotes	cell
T2938	4687-4696	NNS	denotes	receptors
T2939	4696-4697	,	denotes	,
T2940	4698-4702	JJ	denotes	such
T2941	4703-4705	IN	denotes	as
T2942	4706-4715	NNS	denotes	integrins
T2943	4716-4717	-LRB-	denotes	(
T2944	4717-4719	CD	denotes	44
T2945	4719-4720	-RRB-	denotes	)
T2946	4720-4721	,	denotes	,
T2947	4722-4730	NN	denotes	Flk1/KDR
T2948	4731-4739	NN	denotes	receptor
T2949	4740-4741	-LRB-	denotes	(
T2950	4741-4743	CD	denotes	45
T2951	4743-4744	-RRB-	denotes	)
T2952	4745-4748	CC	denotes	and
T2953	4749-4758	JJ	denotes	chemokine
T2954	4759-4768	NNS	denotes	receptors
T2955	4769-4770	-LRB-	denotes	(
T2956	4770-4772	CD	denotes	46
T2957	4772-4773	-RRB-	denotes	)
T2958	4773-4774	.	denotes	.
T2959	4775-4777	PRP	denotes	We
T2960	4778-4783	RB	denotes	first
T2961	4784-4792	VBD	denotes	reported
T2962	4793-4797	IN	denotes	that
T2963	4798-4803	NNP	denotes	NF-κB
T2964	4804-4807	VBD	denotes	was
T2965	4808-4822	RB	denotes	constitutively
T2966	4823-4829	JJ	denotes	active
T2967	4830-4832	IN	denotes	in
T2968	4833-4839	NNP	denotes	Jurkat
T2969	4840-4845	NNS	denotes	cells
T2970	4846-4850	WDT	denotes	that
T2971	4851-4857	RB	denotes	stably
T2972	4858-4867	VBD	denotes	expressed
T2973	4868-4871	DT	denotes	the
T2974	4872-4875	NNP	denotes	Tat
T2975	4876-4880	NN	denotes	gene
T2976	4881-4882	-LRB-	denotes	(
T2977	4882-4884	CD	denotes	47
T2978	4884-4885	-RRB-	denotes	)
T2979	4885-4886	.	denotes	.
T2980	4887-4896	VBG	denotes	Following
T2981	4897-4901	NN	denotes	gene
T2982	4902-4914	NN	denotes	transfection
T2983	4915-4917	CC	denotes	or
T2984	4918-4925	NN	denotes	protein
T2985	4926-4938	NN	denotes	transduction
T2986	4938-4939	,	denotes	,
T2987	4940-4943	NNP	denotes	Tat
T2988	4944-4951	VBD	denotes	induced
T2989	4952-4955	DT	denotes	the
T2990	4956-4959	NNP	denotes	IKK
T2991	4960-4968	NN	denotes	activity
T2992	4969-4972	CC	denotes	and
T2993	4973-4984	JJ	denotes	proteasomal
T2994	4985-4996	NN	denotes	degradation
T2995	4997-4999	IN	denotes	of
T2996	5000-5005	NNP	denotes	IκB-α
T2997	5006-5007	-LRB-	denotes	(
T2998	5007-5009	CD	denotes	48
T2999	5009-5010	-RRB-	denotes	)
T3000	5010-5011	,	denotes	,
T3001	5012-5015	CC	denotes	and
T3002	5016-5025	VBD	denotes	increased
T3003	5026-5029	DT	denotes	the
T3004	5030-5033	NNS	denotes	p65
T3005	5034-5049	JJ	denotes	transcriptional
T3006	5050-5058	NN	denotes	activity
T3007	5059-5061	IN	denotes	by
T3008	5062-5072	VBG	denotes	inhibiting
T3009	5073-5076	DT	denotes	the
T3010	5077-5092	JJ	denotes	SIRT-1-mediated
T3011	5093-5106	NN	denotes	deacetylation
T3012	5107-5109	IN	denotes	of
T3013	5110-5113	CD	denotes	p65
T3014	5114-5120	NNP	denotes	Lys310
T3015	5121-5122	-LRB-	denotes	(
T3016	5122-5124	CD	denotes	49
T3017	5124-5125	-RRB-	denotes	)
T3018	5125-5126	.	denotes	.
T3019	5127-5132	DT	denotes	These
T3020	5133-5141	NNS	denotes	findings
T3021	5142-5151	VBD	denotes	suggested
T3022	5152-5156	IN	denotes	that
T3023	5157-5160	NNP	denotes	Tat
T3024	5161-5170	VBZ	denotes	modulates
T3025	5171-5178	JJ	denotes	crucial
T3026	5179-5186	NNS	denotes	enzymes
T3027	5187-5195	VBN	denotes	involved
T3028	5196-5198	IN	denotes	in
T3029	5199-5204	JJ	denotes	NF-κB
T3030	5205-5214	VBG	denotes	signaling
T3031	5214-5215	:	denotes	;
T3032	5216-5223	RB	denotes	however
T3033	5223-5224	,	denotes	,
T3034	5225-5227	PRP	denotes	it
T3035	5228-5231	VBD	denotes	was
T3036	5232-5239	JJ	denotes	unclear
T3037	5240-5243	WRB	denotes	how
T3038	5244-5247	NNP	denotes	Tat
T3039	5248-5253	MD	denotes	could
T3040	5254-5261	VB	denotes	subvert
T3041	5262-5265	DT	denotes	the
T3042	5266-5274	JJ	denotes	negative
T3043	5275-5283	NN	denotes	feedback
T3044	5284-5286	IN	denotes	of
T3045	5287-5292	NNP	denotes	NF-κB
T3046	5292-5293	,	denotes	,
T3047	5294-5299	WDT	denotes	which
T3048	5300-5302	VBZ	denotes	is
T3049	5303-5309	RB	denotes	mainly
T3050	5310-5319	JJ	denotes	dependent
T3051	5320-5322	IN	denotes	on
T3052	5323-5325	FW	denotes	de
T3053	5326-5330	FW	denotes	novo
T3054	5331-5340	NN	denotes	synthesis
T3055	5341-5343	IN	denotes	of
T3056	5344-5349	NNP	denotes	IκB-α
T3057	5350-5351	-LRB-	denotes	(
T3058	5351-5356	CD	denotes	15,17
T3059	5356-5357	-RRB-	denotes	)
T3060	5357-5358	.	denotes	.
T3061	5359-5361	PRP	denotes	We
T3062	5362-5372	RB	denotes	previously
T3063	5373-5378	VBD	denotes	found
T3064	5379-5383	IN	denotes	that
T3065	5384-5389	JJ	denotes	IκB-α
T3066	5390-5395	NNS	denotes	binds
T3067	5396-5398	TO	denotes	to
T3068	5399-5402	NNP	denotes	Tat
T3069	5403-5406	CC	denotes	and
T3070	5407-5415	VBZ	denotes	promotes
T3071	5416-5419	DT	denotes	the
T3072	5420-5427	JJ	denotes	nuclear
T3073	5428-5434	NN	denotes	export
T3074	5435-5437	IN	denotes	of
T3075	5438-5441	DT	denotes	the
T3076	5442-5447	JJ	denotes	viral
T3077	5448-5462	NN	denotes	transactivator
T3078	5463-5464	-LRB-	denotes	(
T3079	5464-5469	CD	denotes	50,51
T3080	5469-5470	-RRB-	denotes	)
T3081	5470-5471	.	denotes	.
T3082	5472-5474	IN	denotes	In
T3083	5475-5479	DT	denotes	this
T3084	5480-5485	NN	denotes	study
T3085	5485-5486	,	denotes	,
T3086	5487-5489	PRP	denotes	we
T3087	5490-5496	VBP	denotes	report
T3088	5497-5501	IN	denotes	that
T3089	5502-5505	NNP	denotes	Tat
T3090	5506-5517	VBZ	denotes	counteracts
T3091	5518-5521	DT	denotes	the
T3092	5522-5537	JJ	denotes	post-activation
T3093	5538-5542	NN	denotes	turn
T3094	5543-5546	IN	denotes	off
T3095	5547-5549	IN	denotes	of
T3096	5550-5555	NN	denotes	NF-κB
T3097	5556-5563	IN	denotes	through
T3098	5564-5570	JJ	denotes	direct
T3099	5571-5582	NN	denotes	interaction
T3100	5583-5587	IN	denotes	with
T3101	5588-5593	NNP	denotes	IκB-α
T3102	5594-5597	CC	denotes	and
T3103	5598-5601	CD	denotes	p65
T3104	5601-5602	,	denotes	,
T3105	5603-5608	WDT	denotes	which
T3106	5609-5617	VBZ	denotes	enhances
T3107	5618-5621	DT	denotes	the
T3108	5622-5625	NNP	denotes	DNA
T3109	5626-5633	JJ	denotes	binding
T3110	5634-5637	CC	denotes	and
T3111	5638-5653	JJ	denotes	transcriptional
T3112	5654-5662	NN	denotes	activity
T3113	5663-5665	IN	denotes	of
T3114	5666-5669	DT	denotes	the
T3115	5670-5675	NNP	denotes	NF-κB
T3116	5676-5683	NN	denotes	complex
T3117	5683-5684	.	denotes	.
T3118	5685-5688	DT	denotes	The
T3119	5689-5692	JJ	denotes	new
T3120	5693-5702	NN	denotes	mechanism
T3121	5703-5705	IN	denotes	of
T3122	5706-5711	JJ	denotes	NF-κB
T3123	5712-5724	NN	denotes	deregulation
T3124	5725-5729	RB	denotes	here
T3125	5730-5739	VBD	denotes	described
T3126	5740-5743	MD	denotes	may
T3127	5744-5751	VB	denotes	provide
T3128	5752-5759	JJ	denotes	further
T3129	5760-5768	NNS	denotes	insights
T3130	5769-5773	IN	denotes	into
T3131	5774-5777	DT	denotes	the
T3132	5778-5785	JJ	denotes	chronic
T3133	5786-5792	JJ	denotes	immune
T3134	5793-5803	NN	denotes	activation
T3135	5804-5806	IN	denotes	of
T3136	5807-5812	JJ	denotes	HIV-1
T3137	5813-5822	NN	denotes	infection
T3138	5822-5823	.	denotes	.
T4596	5848-5856	NNP	denotes	Plasmids
T4597	5857-5860	DT	denotes	The
T4598	5861-5869	NNS	denotes	plasmids
T4599	5870-5886	NN	denotes	pcDNA-3xHA-IκB-α
T4600	5886-5887	,	denotes	,
T4601	5888-5903	NN	denotes	p3xFLAG-CMV-Tat
T4602	5903-5904	,	denotes	,
T4603	5905-5920	NN	denotes	p3xFLAG-CMV-Tat
T4604	5921-5922	NNP	denotes	C
T4605	5922-5923	-LRB-	denotes	(
T4606	5923-5931	CD	denotes	22,25,27
T4607	5931-5932	-RRB-	denotes	)
T4608	5932-5933	DT	denotes	A
T4609	5933-5934	,	denotes	,
T4610	5935-5950	JJ	denotes	p3xFLAG-CMV-Tat
T4611	5951-5952	NN	denotes	R
T4612	5952-5953	-LRB-	denotes	(
T4613	5953-5970	CD	denotes	49,52,53,55,56,57
T4614	5970-5971	-RRB-	denotes	)
T4615	5971-5972	DT	denotes	A
T4616	5972-5973	,	denotes	,
T4617	5974-5985	NN	denotes	pGEX-2T-Tat
T4618	5985-5986	,	denotes	,
T4619	5987-5998	NN	denotes	pGEX-2T-Tat
T4620	5999-6000	NNP	denotes	C
T4621	6000-6001	-LRB-	denotes	(
T4622	6001-6009	CD	denotes	22,25,27
T4623	6009-6010	-RRB-	denotes	)
T4624	6010-6011	DT	denotes	A
T4625	6012-6015	CC	denotes	and
T4626	6016-6027	JJ	denotes	pGEX-2T-Tat
T4627	6028-6029	NN	denotes	R
T4628	6029-6030	-LRB-	denotes	(
T4629	6030-6047	CD	denotes	49,52,53,55,56,57
T4630	6047-6048	-RRB-	denotes	)
T4631	6048-6049	DT	denotes	A
T4632	6050-6054	VBD	denotes	were
T4633	6055-6065	RB	denotes	previously
T4634	6066-6075	VBN	denotes	described
T4635	6076-6077	-LRB-	denotes	(
T4636	6077-6079	CD	denotes	50
T4637	6079-6080	-RRB-	denotes	)
T4638	6080-6081	.	denotes	.
T4639	6082-6085	DT	denotes	The
T4640	6086-6094	NNS	denotes	plasmids
T4641	6095-6101	JJ	denotes	pNL4-3
T4642	6101-6102	.	denotes	.
T4643	6102-6109	SYM	denotes	Luc.R-E
T4644	6109-6110	:	denotes	-
T4645	6111-6114	CC	denotes	and
T4646	6115-6124	JJ	denotes	pHXB2-env
T4647	6125-6129	VBD	denotes	were
T4648	6130-6138	VBN	denotes	obtained
T4649	6139-6143	IN	denotes	from
T4650	6144-6147	DT	denotes	the
T4651	6148-6152	NNP	denotes	AIDS
T4652	6153-6161	NNP	denotes	Research
T4653	6162-6163	CC	denotes	&
T4654	6164-6173	NNP	denotes	Reference
T4655	6174-6181	NNP	denotes	Reagent
T4656	6182-6189	NNP	denotes	Program
T4657	6189-6190	,	denotes	,
T4658	6191-6199	NNP	denotes	Division
T4659	6200-6202	IN	denotes	of
T4660	6203-6207	NNP	denotes	AIDS
T4661	6207-6208	,	denotes	,
T4662	6209-6214	NNP	denotes	NIAID
T4663	6214-6215	,	denotes	,
T4664	6216-6219	NNP	denotes	NIH
T4665	6219-6220	,	denotes	,
T4666	6221-6224	NNP	denotes	USA
T4667	6224-6225	:	denotes	;
T4668	6226-6232	NNP	denotes	pκBluc
T4669	6233-6236	CC	denotes	and
T4670	6237-6246	JJ	denotes	pSV-β-Gal
T4671	6247-6251	VBD	denotes	were
T4672	6252-6261	VBN	denotes	purchased
T4673	6262-6266	IN	denotes	from
T4674	6267-6274	NNP	denotes	Promega
T4675	6275-6276	-LRB-	denotes	(
T4676	6276-6283	NNP	denotes	Madison
T4677	6283-6284	,	denotes	,
T4678	6285-6287	NNP	denotes	WI
T4679	6287-6288	,	denotes	,
T4680	6289-6292	NNP	denotes	USA
T4681	6292-6293	-RRB-	denotes	)
T4682	6293-6294	.	denotes	.
T4683	6295-6298	DT	denotes	The
T4684	6299-6307	NNS	denotes	plasmids
T4685	6308-6315	NNP	denotes	pRc/CMV
T4686	6315-6317	CD	denotes	-3
T4687	6317-6324	NNP	denotes	xHA-p65
T4688	6324-6325	,	denotes	,
T4689	6326-6333	NNP	denotes	pRc/CMV
T4690	6333-6335	CD	denotes	-3
T4691	6335-6344	NN	denotes	xHA-p65ΔC
T4692	6344-6345	-LRB-	denotes	(
T4693	6345-6346	CD	denotes	1
T4694	6347-6350	CD	denotes	318
T4695	6350-6351	-RRB-	denotes	)
T4696	6351-6352	,	denotes	,
T4697	6353-6360	NNP	denotes	pRc/CMV
T4698	6360-6362	CD	denotes	-3
T4699	6362-6371	NNP	denotes	xHA-p65ΔN
T4700	6371-6372	-LRB-	denotes	(
T4701	6372-6375	CD	denotes	122
T4702	6376-6379	CD	denotes	551
T4703	6379-6380	-RRB-	denotes	)
T4704	6380-6381	,	denotes	,
T4705	6382-6397	JJ	denotes	p3xFLAG-CMV-Tat
T4706	6398-6399	NNP	denotes	T
T4707	6399-6400	,	denotes	,
T4708	6400-6401	NNP	denotes	N
T4709	6401-6402	-LRB-	denotes	(
T4710	6402-6407	CD	denotes	23,24
T4711	6407-6408	-RRB-	denotes	)
T4712	6408-6409	DT	denotes	A
T4713	6409-6410	,	denotes	,
T4714	6411-6426	JJ	denotes	p3xFLAG-CMV-Tat
T4715	6427-6428	NNP	denotes	K
T4716	6428-6429	-LRB-	denotes	(
T4717	6429-6434	CD	denotes	50,51
T4718	6434-6435	-RRB-	denotes	)
T4719	6435-6436	DT	denotes	A
T4720	6436-6437	,	denotes	,
T4721	6438-6449	JJ	denotes	pGEX-2T-Tat
T4722	6450-6451	NNP	denotes	T
T4723	6451-6452	,	denotes	,
T4724	6452-6453	NNP	denotes	N
T4725	6453-6454	-LRB-	denotes	(
T4726	6454-6459	CD	denotes	23,24
T4727	6459-6460	-RRB-	denotes	)
T4728	6460-6461	DT	denotes	A
T4729	6461-6462	,	denotes	,
T4730	6463-6474	JJ	denotes	pGEX-2T-Tat
T4731	6475-6476	NNP	denotes	K
T4732	6476-6477	-LRB-	denotes	(
T4733	6477-6482	CD	denotes	50,51
T4734	6482-6483	-RRB-	denotes	)
T4735	6483-6484	DT	denotes	A
T4736	6485-6488	CC	denotes	and
T4737	6489-6495	JJ	denotes	pNL4-3
T4738	6495-6496	.	denotes	.
T4739	6496-6504	NN	denotes	FLAG-Tat
T4740	6504-6505	.	denotes	.
T4741	6505-6508	SYM	denotes	R-E
T4742	6508-6509	:	denotes	-
T4743	6510-6514	VBD	denotes	were
T4744	6515-6524	VBN	denotes	generated
T4745	6525-6527	IN	denotes	as
T4746	6528-6537	VBN	denotes	described
T4747	6538-6540	IN	denotes	in
T4748	6541-6554	NNP	denotes	Supplementary
T4749	6555-6559	NNP	denotes	Data
T4750	6559-6560	.	denotes	.
T5199	6562-6567	NNS	denotes	Cells
T5200	6567-6568	,	denotes	,
T5201	6569-6581	NN	denotes	transfection
T5202	6581-6582	,	denotes	,
T5203	6583-6593	NNS	denotes	treatments
T5204	6594-6597	CC	denotes	and
T5205	6598-6608	NN	denotes	luciferase
T5206	6609-6614	NN	denotes	assay
T5207	6615-6619	NNP	denotes	HeLa
T5208	6619-6620	,	denotes	,
T5209	6621-6624	CD	denotes	p50
T5210	6624-6625	FW	denotes	−
T5211	6625-6626	FW	denotes	/
T5212	6626-6627	FW	denotes	−
T5213	6627-6630	FW	denotes	p65
T5214	6630-6631	FW	denotes	−
T5215	6631-6632	FW	denotes	/
T5216	6632-6633	FW	denotes	−
T5217	6634-6639	NN	denotes	mouse
T5218	6640-6649	JJ	denotes	embryonic
T5219	6650-6661	NNS	denotes	fibroblasts
T5220	6662-6663	-LRB-	denotes	(
T5221	6663-6667	NNS	denotes	MEFs
T5222	6667-6668	-RRB-	denotes	)
T5223	6669-6670	-LRB-	denotes	(
T5224	6670-6672	CD	denotes	52
T5225	6672-6673	-RRB-	denotes	)
T5226	6674-6677	CC	denotes	and
T5227	6678-6682	JJ	denotes	293T
T5228	6683-6688	NNS	denotes	cells
T5229	6689-6693	VBD	denotes	were
T5230	6694-6702	VBN	denotes	cultured
T5231	6703-6705	IN	denotes	in
T5232	6706-6714	NNP	denotes	Dulbecco
T5233	6714-6716	POS	denotes	's
T5234	6717-6725	VBN	denotes	modified
T5235	6726-6731	NNP	denotes	Eagle
T5236	6731-6733	POS	denotes	's
T5237	6734-6740	NN	denotes	medium
T5238	6740-6741	:	denotes	;
T5239	6742-6748	NNP	denotes	Jurkat
T5240	6748-6749	,	denotes	,
T5241	6750-6754	NNP	denotes	U937
T5242	6755-6760	NNS	denotes	cells
T5243	6761-6764	CC	denotes	and
T5244	6765-6770	JJ	denotes	human
T5245	6771-6781	JJ	denotes	peripheral
T5246	6782-6787	NN	denotes	blood
T5247	6788-6799	NN	denotes	mononuclear
T5248	6800-6805	NNS	denotes	cells
T5249	6806-6807	-LRB-	denotes	(
T5250	6807-6812	NNS	denotes	PBMCs
T5251	6812-6813	-RRB-	denotes	)
T5252	6814-6818	VBD	denotes	were
T5253	6819-6827	VBN	denotes	cultured
T5254	6828-6830	IN	denotes	in
T5255	6831-6835	NNP	denotes	RPMI
T5256	6836-6840	CD	denotes	1640
T5257	6840-6841	.	denotes	.
T5258	6842-6847	NNS	denotes	PBMCs
T5259	6848-6852	VBD	denotes	were
T5260	6853-6861	VBN	denotes	isolated
T5261	6862-6864	IN	denotes	as
T5262	6865-6875	RB	denotes	previously
T5263	6876-6885	VBN	denotes	described
T5264	6886-6887	-LRB-	denotes	(
T5265	6887-6889	CD	denotes	53
T5266	6889-6890	-RRB-	denotes	)
T5267	6890-6891	.	denotes	.
T5268	6892-6897	NNP	denotes	Media
T5269	6898-6902	VBD	denotes	were
T5270	6903-6915	VBN	denotes	supplemented
T5271	6916-6920	IN	denotes	with
T5272	6921-6923	CD	denotes	10
T5273	6923-6924	NN	denotes	%
T5274	6925-6941	JJ	denotes	heat-inactivated
T5275	6942-6947	JJ	denotes	fetal
T5276	6948-6952	NN	denotes	calf
T5277	6953-6958	NN	denotes	serum
T5278	6959-6962	CC	denotes	and
T5279	6963-6964	CD	denotes	2
T5280	6965-6967	NNP	denotes	mM
T5281	6968-6979	NN	denotes	l-glutamine
T5282	6980-6981	-LRB-	denotes	(
T5283	6981-6986	NNP	denotes	Lonza
T5284	6987-6994	NNP	denotes	Cologne
T5285	6995-6997	NNP	denotes	AG
T5286	6997-6998	,	denotes	,
T5287	6999-7006	NNP	denotes	Germany
T5288	7006-7007	-RRB-	denotes	)
T5289	7007-7008	.	denotes	.
T5290	7009-7013	NNP	denotes	HeLa
T5291	7013-7014	,	denotes	,
T5292	7015-7018	CD	denotes	p50
T5293	7018-7019	FW	denotes	−
T5294	7019-7020	FW	denotes	/
T5295	7020-7021	FW	denotes	−
T5296	7021-7024	FW	denotes	p65
T5297	7024-7025	FW	denotes	−
T5298	7025-7026	FW	denotes	/
T5299	7026-7027	FW	denotes	−
T5300	7028-7032	NNPS	denotes	MEFs
T5301	7033-7036	CC	denotes	and
T5302	7037-7041	NNP	denotes	293T
T5303	7042-7046	VBD	denotes	were
T5304	7047-7058	VBN	denotes	transfected
T5305	7059-7063	IN	denotes	with
T5306	7064-7067	NN	denotes	DNA
T5307	7068-7070	IN	denotes	by
T5308	7071-7076	VBG	denotes	using
T5309	7077-7083	NNP	denotes	FuGENE
T5310	7084-7086	NNP	denotes	HD
T5311	7087-7088	-LRB-	denotes	(
T5312	7088-7093	NNP	denotes	Roche
T5313	7094-7104	NNP	denotes	Diagnostic
T5314	7105-7109	NNP	denotes	GmbH
T5315	7109-7110	,	denotes	,
T5316	7111-7119	NNP	denotes	Mannheim
T5317	7119-7120	,	denotes	,
T5318	7121-7128	NNP	denotes	Germany
T5319	7128-7129	-RRB-	denotes	)
T5320	7129-7130	,	denotes	,
T5321	7131-7140	VBG	denotes	according
T5322	7141-7143	TO	denotes	to
T5323	7144-7147	DT	denotes	the
T5324	7148-7160	NN	denotes	manufacturer
T5325	7160-7162	POS	denotes	's
T5326	7163-7171	NN	denotes	protocol
T5327	7171-7172	:	denotes	;
T5328	7173-7178	JJ	denotes	total
T5329	7179-7182	NNP	denotes	DNA
T5330	7183-7190	NNS	denotes	amounts
T5331	7191-7195	VBD	denotes	were
T5332	7196-7205	VBN	denotes	equalized
T5333	7206-7208	IN	denotes	by
T5334	7209-7221	NN	denotes	transfection
T5335	7222-7224	IN	denotes	of
T5336	7225-7232	NNP	denotes	pRc/CMV
T5337	7233-7238	JJ	denotes	empty
T5338	7239-7245	NN	denotes	vector
T5339	7246-7247	-LRB-	denotes	(
T5340	7247-7257	NNP	denotes	Invitrogen
T5341	7257-7258	,	denotes	,
T5342	7259-7267	NNP	denotes	Carlsbad
T5343	7267-7268	,	denotes	,
T5344	7269-7271	NNP	denotes	CA
T5345	7271-7272	,	denotes	,
T5346	7273-7276	NNP	denotes	USA
T5347	7276-7277	-RRB-	denotes	)
T5348	7277-7278	.	denotes	.
T5349	7279-7282	IN	denotes	For
T5350	7283-7300	NN	denotes	pulse-stimulation
T5351	7300-7301	,	denotes	,
T5352	7302-7306	NNP	denotes	HeLa
T5353	7307-7312	NNS	denotes	cells
T5354	7313-7317	VBD	denotes	were
T5355	7318-7325	VBN	denotes	treated
T5356	7326-7330	IN	denotes	with
T5357	7331-7338	NN	denotes	phorbol
T5358	7339-7351	JJ	denotes	12-myristate
T5359	7352-7362	JJ	denotes	13-acetate
T5360	7363-7364	-LRB-	denotes	(
T5361	7364-7367	NNP	denotes	PMA
T5362	7367-7368	:	denotes	;
T5363	7369-7382	NNP	denotes	Sigma-Aldrich
T5364	7382-7383	,	denotes	,
T5365	7384-7386	NNP	denotes	St
T5366	7387-7392	NNP	denotes	Louis
T5367	7392-7393	,	denotes	,
T5368	7394-7396	NNP	denotes	MO
T5369	7396-7397	,	denotes	,
T5370	7398-7401	NNP	denotes	USA
T5371	7401-7402	-RRB-	denotes	)
T5372	7403-7404	-LRB-	denotes	(
T5373	7404-7406	CD	denotes	20
T5374	7407-7412	NN	denotes	ng/ml
T5375	7412-7413	-RRB-	denotes	)
T5376	7414-7417	IN	denotes	for
T5377	7418-7419	CD	denotes	5
T5378	7420-7423	NN	denotes	min
T5379	7423-7424	,	denotes	,
T5380	7425-7427	CC	denotes	or
T5381	7428-7433	NN	denotes	tumor
T5382	7434-7442	NN	denotes	necrosis
T5383	7443-7451	NN	denotes	factor-α
T5384	7452-7453	-LRB-	denotes	(
T5385	7453-7458	JJ	denotes	TNF-α
T5386	7458-7459	:	denotes	;
T5387	7460-7473	NNP	denotes	Sigma-Aldrich
T5388	7473-7474	-RRB-	denotes	)
T5389	7475-7476	-LRB-	denotes	(
T5390	7476-7478	CD	denotes	20
T5391	7479-7484	NN	denotes	ng/ml
T5392	7484-7485	-RRB-	denotes	)
T5393	7486-7489	IN	denotes	for
T5394	7490-7492	CD	denotes	30
T5395	7493-7496	NN	denotes	min
T5396	7496-7497	,	denotes	,
T5397	7498-7504	VBD	denotes	washed
T5398	7505-7510	RB	denotes	twice
T5399	7511-7513	IN	denotes	in
T5400	7514-7522	JJ	denotes	complete
T5401	7523-7530	NN	denotes	culture
T5402	7531-7537	NN	denotes	medium
T5403	7538-7541	CC	denotes	and
T5404	7542-7546	RB	denotes	then
T5405	7547-7555	VBD	denotes	returned
T5406	7556-7558	TO	denotes	to
T5407	7559-7566	NN	denotes	culture
T5408	7566-7567	.	denotes	.
T5409	7568-7571	IN	denotes	For
T5410	7572-7582	NN	denotes	luciferase
T5411	7583-7589	NNS	denotes	assays
T5412	7589-7590	,	denotes	,
T5413	7591-7600	JJ	denotes	pSV-β-Gal
T5414	7601-7604	VBD	denotes	was
T5415	7605-7619	VBN	denotes	co-transfected
T5416	7620-7624	IN	denotes	with
T5417	7625-7631	NNP	denotes	pκBluc
T5418	7632-7634	TO	denotes	to
T5419	7635-7642	VB	denotes	monitor
T5420	7643-7646	DT	denotes	the
T5421	7647-7659	NN	denotes	transfection
T5422	7660-7670	NN	denotes	efficiency
T5423	7670-7671	.	denotes	.
T5424	7672-7688	JJ	denotes	Forty-eight-hour
T5425	7689-7706	NN	denotes	post-transfection
T5426	7706-7707	,	denotes	,
T5427	7708-7713	NNS	denotes	cells
T5428	7714-7718	VBD	denotes	were
T5429	7719-7724	VBN	denotes	lysed
T5430	7725-7727	IN	denotes	in
T5431	7728-7733	NN	denotes	lysis
T5432	7734-7740	NN	denotes	buffer
T5433	7741-7743	IN	denotes	of
T5434	7744-7748	NNP	denotes	Dual
T5435	7749-7754	NNP	denotes	Light
T5436	7755-7765	NNP	denotes	Luciferase
T5437	7766-7772	NNP	denotes	System
T5438	7773-7774	-LRB-	denotes	(
T5439	7774-7780	NNP	denotes	Tropix
T5440	7780-7781	,	denotes	,
T5441	7782-7789	NNP	denotes	Bedford
T5442	7789-7790	,	denotes	,
T5443	7791-7793	NNP	denotes	MA
T5444	7793-7794	,	denotes	,
T5445	7795-7798	NNP	denotes	USA
T5446	7798-7799	-RRB-	denotes	)
T5447	7800-7803	CC	denotes	and
T5448	7804-7807	DT	denotes	the
T5449	7808-7818	NN	denotes	luciferase
T5450	7819-7822	CC	denotes	and
T5451	7823-7838	JJ	denotes	β-galactosidase
T5452	7839-7849	NNS	denotes	activities
T5453	7850-7854	VBD	denotes	were
T5454	7855-7864	VBN	denotes	evaluated
T5455	7865-7867	IN	denotes	by
T5456	7868-7873	VBG	denotes	using
T5457	7874-7878	NNP	denotes	Dual
T5458	7879-7884	NNP	denotes	Light
T5459	7885-7895	NNP	denotes	Luciferase
T5460	7896-7902	NNP	denotes	System
T5461	7903-7904	-LRB-	denotes	(
T5462	7904-7910	NNP	denotes	Tropix
T5463	7910-7911	-RRB-	denotes	)
T5464	7912-7914	IN	denotes	in
T5465	7915-7916	DT	denotes	a
T5466	7917-7931	NN	denotes	bioluminometer
T5467	7932-7933	-LRB-	denotes	(
T5468	7933-7939	NNP	denotes	Turner
T5469	7940-7949	NNP	denotes	Biosystem
T5470	7949-7950	,	denotes	,
T5471	7951-7960	NNP	denotes	Sunnyvale
T5472	7960-7961	,	denotes	,
T5473	7962-7964	NNP	denotes	CA
T5474	7964-7965	,	denotes	,
T5475	7966-7969	NNP	denotes	USA
T5476	7969-7970	-RRB-	denotes	)
T5477	7970-7971	.	denotes	.
T5478	7972-7975	DT	denotes	The
T5479	7976-7981	NN	denotes	ratio
T5480	7982-7984	IN	denotes	of
T5481	7985-7992	RB	denotes	firefly
T5482	7993-8003	JJ	denotes	luciferase
T5483	8004-8012	NN	denotes	activity
T5484	8013-8015	TO	denotes	to
T5485	8016-8031	JJ	denotes	β-galactosidase
T5486	8032-8040	NN	denotes	activity
T5487	8041-8044	VBD	denotes	was
T5488	8045-8054	VBN	denotes	expressed
T5489	8055-8057	IN	denotes	as
T5490	8058-8066	JJ	denotes	relative
T5491	8067-8072	JJ	denotes	light
T5492	8073-8078	NNS	denotes	units
T5493	8078-8079	.	denotes	.
T5824	8081-8084	NNP	denotes	RNA
T5825	8085-8097	NN	denotes	interference
T5826	8098-8104	NNP	denotes	Jurkat
T5827	8105-8107	CC	denotes	or
T5828	8108-8112	NNP	denotes	U937
T5829	8113-8118	NNS	denotes	cells
T5830	8119-8123	VBD	denotes	were
T5831	8124-8135	VBN	denotes	transfected
T5832	8136-8138	IN	denotes	by
T5833	8139-8154	NN	denotes	electroporation
T5834	8155-8160	VBG	denotes	using
T5835	8161-8162	DT	denotes	a
T5836	8163-8170	NNP	denotes	Bio-Rad
T5837	8171-8180	NN	denotes	apparatus
T5838	8181-8182	-LRB-	denotes	(
T5839	8182-8189	NNP	denotes	Bio-Rad
T5840	8190-8202	NNPS	denotes	Laboratories
T5841	8202-8203	,	denotes	,
T5842	8204-8212	NNP	denotes	Hercules
T5843	8212-8213	,	denotes	,
T5844	8214-8216	NNP	denotes	CA
T5845	8216-8217	,	denotes	,
T5846	8218-8221	NNP	denotes	USA
T5847	8221-8222	-RRB-	denotes	)
T5848	8222-8223	.	denotes	.
T5849	8224-8231	RB	denotes	Briefly
T5850	8231-8232	,	denotes	,
T5851	8233-8241	NNS	denotes	aliquots
T5852	8242-8243	-LRB-	denotes	(
T5853	8243-8244	CD	denotes	5
T5854	8245-8246	CD	denotes	×
T5855	8247-8250	CD	denotes	106
T5856	8251-8256	NNS	denotes	cells
T5857	8256-8257	-RRB-	denotes	)
T5858	8258-8262	VBD	denotes	were
T5859	8263-8272	VBN	denotes	suspended
T5860	8273-8275	IN	denotes	in
T5861	8276-8279	CD	denotes	0.3
T5862	8280-8282	NN	denotes	ml
T5863	8283-8285	IN	denotes	of
T5864	8286-8290	NNP	denotes	RPMI
T5865	8291-8295	CD	denotes	1640
T5866	8296-8308	VBN	denotes	supplemented
T5867	8309-8313	IN	denotes	with
T5868	8314-8316	CD	denotes	20
T5869	8316-8317	NN	denotes	%
T5870	8318-8323	JJ	denotes	fetal
T5871	8324-8328	NN	denotes	calf
T5872	8329-8334	NN	denotes	serum
T5873	8335-8338	CC	denotes	and
T5874	8339-8348	VBN	denotes	subjected
T5875	8349-8351	TO	denotes	to
T5876	8352-8353	DT	denotes	a
T5877	8354-8360	JJ	denotes	double
T5878	8361-8371	JJ	denotes	electrical
T5879	8372-8377	NN	denotes	pulse
T5880	8378-8379	-LRB-	denotes	(
T5881	8379-8383	CD	denotes	0.22
T5882	8384-8385	NNP	denotes	V
T5883	8385-8386	,	denotes	,
T5884	8387-8390	CD	denotes	960
T5885	8391-8393	NN	denotes	µF
T5886	8393-8394	-RRB-	denotes	)
T5887	8395-8397	IN	denotes	in
T5888	8398-8401	DT	denotes	the
T5889	8402-8410	NN	denotes	presence
T5890	8411-8413	IN	denotes	of
T5891	8414-8422	JJ	denotes	annealed
T5892	8423-8428	NNP	denotes	siRNA
T5893	8429-8430	-LRB-	denotes	(
T5894	8430-8433	CD	denotes	200
T5895	8434-8438	NN	denotes	pmol
T5896	8438-8439	-RRB-	denotes	)
T5897	8439-8440	:	denotes	;
T5898	8441-8455	VBD	denotes	electroporated
T5899	8456-8461	NNS	denotes	cells
T5900	8462-8466	VBD	denotes	were
T5901	8467-8473	VBN	denotes	washed
T5902	8474-8477	CC	denotes	and
T5903	8478-8486	VBN	denotes	cultured
T5904	8487-8489	IN	denotes	in
T5905	8490-8498	JJ	denotes	complete
T5906	8499-8505	NN	denotes	medium
T5907	8505-8506	.	denotes	.
T5908	8507-8510	NNP	denotes	RNA
T5909	8511-8523	NN	denotes	interference
T5910	8524-8527	VBD	denotes	was
T5911	8528-8537	VBN	denotes	performed
T5912	8538-8542	IN	denotes	with
T5913	8542-8543	:	denotes	:
T5914	8544-8549	NNP	denotes	siRNA
T5915	8550-8553	NNP	denotes	Tat
T5916	8554-8559	NN	denotes	sense
T5917	8559-8560	,	denotes	,
T5918	8561-8582	NNP	denotes	CUGCUUGUACCAAUUGCUAUU
T5919	8583-8586	CC	denotes	and
T5920	8587-8592	NNP	denotes	siRNA
T5921	8593-8596	NNP	denotes	Tat
T5922	8597-8606	NN	denotes	antisense
T5923	8606-8607	,	denotes	,
T5924	8608-8629	NNP	denotes	UAGCAAUUGGUACAAGCAGUU
T5925	8629-8630	:	denotes	;
T5926	8631-8636	NNP	denotes	siRNA
T5927	8637-8644	NN	denotes	control
T5928	8645-8650	NN	denotes	sense
T5929	8650-8651	,	denotes	,
T5930	8652-8664	NNP	denotes	CUGCUUGUCACA
T5931	8665-8674	NNP	denotes	AUUGCUAUU
T5932	8675-8678	CC	denotes	and
T5933	8679-8684	NNP	denotes	siRNA
T5934	8685-8692	NN	denotes	control
T5935	8693-8702	NN	denotes	antisense
T5936	8702-8703	,	denotes	,
T5937	8704-8725	NNP	denotes	UAGCAAUUGUGACAAGCAGUU
T5938	8725-8726	.	denotes	.
T5939	8727-8730	NNP	denotes	RNA
T5940	8731-8743	NN	denotes	interference
T5941	8744-8746	IN	denotes	of
T5942	8747-8750	CD	denotes	p65
T5943	8751-8754	CC	denotes	and
T5944	8755-8760	NNP	denotes	IκB-α
T5945	8761-8764	VBD	denotes	was
T5946	8765-8774	VBN	denotes	performed
T5947	8775-8779	IN	denotes	with
T5948	8780-8785	NNP	denotes	SMART
T5949	8786-8790	NN	denotes	pool
T5950	8791-8796	NNP	denotes	siRNA
T5951	8797-8800	CD	denotes	p65
T5952	8801-8804	CC	denotes	and
T5953	8805-8810	NNP	denotes	IκB-α
T5954	8811-8812	-LRB-	denotes	(
T5955	8812-8821	NNP	denotes	Dharmacon
T5956	8821-8822	,	denotes	,
T5957	8823-8830	NNP	denotes	Chicago
T5958	8830-8831	,	denotes	,
T5959	8832-8834	NNP	denotes	IL
T5960	8834-8835	,	denotes	,
T5961	8836-8839	NNP	denotes	USA
T5962	8839-8840	-RRB-	denotes	)
T5963	8840-8841	.	denotes	.
T6139	8843-8854	NNP	denotes	Pseudotyped
T6140	8855-8862	NNS	denotes	virions
T6141	8863-8866	CC	denotes	and
T6142	8867-8873	JJ	denotes	single
T6143	8874-8879	NN	denotes	round
T6144	8880-8889	NN	denotes	infection
T6145	8890-8894	JJ	denotes	293T
T6146	8895-8900	NNS	denotes	cells
T6147	8901-8902	-LRB-	denotes	(
T6148	8902-8903	CD	denotes	1
T6149	8904-8905	CD	denotes	×
T6150	8906-8909	CD	denotes	107
T6151	8909-8910	-RRB-	denotes	)
T6152	8911-8915	VBD	denotes	were
T6153	8916-8927	VBN	denotes	transfected
T6154	8928-8932	IN	denotes	with
T6155	8933-8939	JJ	denotes	pNL4-3
T6156	8939-8940	.	denotes	.
T6157	8940-8947	SYM	denotes	Luc.R-E
T6158	8947-8948	:	denotes	-
T6159	8949-8951	CC	denotes	or
T6160	8952-8958	JJ	denotes	pNL4-3
T6161	8958-8959	.	denotes	.
T6162	8959-8967	NN	denotes	FLAG-Tat
T6163	8967-8968	.	denotes	.
T6164	8968-8971	SYM	denotes	R-E
T6165	8971-8972	:	denotes	-
T6166	8973-8974	-LRB-	denotes	(
T6167	8974-8976	CD	denotes	10
T6168	8977-8979	NN	denotes	µg
T6169	8979-8980	-RRB-	denotes	)
T6170	8981-8989	RB	denotes	together
T6171	8990-8994	IN	denotes	with
T6172	8995-9000	NNP	denotes	pHXB2
T6173	9001-9004	NNP	denotes	Env
T6174	9005-9006	-LRB-	denotes	(
T6175	9006-9008	CD	denotes	10
T6176	9009-9011	NN	denotes	µg
T6177	9011-9012	-RRB-	denotes	)
T6178	9012-9013	,	denotes	,
T6179	9014-9017	CC	denotes	and
T6180	9018-9022	JJ	denotes	48-h
T6181	9023-9040	JJ	denotes	post-transfection
T6182	9041-9045	NN	denotes	cell
T6183	9046-9057	NN	denotes	supernatant
T6184	9058-9061	VBD	denotes	was
T6185	9062-9071	VBN	denotes	collected
T6186	9071-9072	.	denotes	.
T6187	9073-9086	JJ	denotes	Enzyme-linked
T6188	9087-9100	JJ	denotes	immunosorbent
T6189	9101-9106	NN	denotes	assay
T6190	9107-9108	-LRB-	denotes	(
T6191	9108-9113	NNP	denotes	ELISA
T6192	9113-9114	-RRB-	denotes	)
T6193	9115-9120	VBG	denotes	using
T6194	9121-9129	JJ	denotes	anti-p24
T6195	9130-9138	NN	denotes	antibody
T6196	9139-9147	VBN	denotes	measured
T6197	9148-9154	NN	denotes	virion
T6198	9155-9168	NN	denotes	concentration
T6199	9168-9169	.	denotes	.
T6200	9170-9175	NNS	denotes	PBMCs
T6201	9175-9176	,	denotes	,
T6202	9177-9183	NNP	denotes	Jurkat
T6203	9184-9186	CC	denotes	or
T6204	9187-9191	NNP	denotes	U937
T6205	9192-9197	NNS	denotes	cells
T6206	9198-9199	-LRB-	denotes	(
T6207	9199-9200	CD	denotes	5
T6208	9201-9202	RB	denotes	×
T6209	9203-9206	CD	denotes	107
T6210	9206-9207	-RRB-	denotes	)
T6211	9208-9212	VBD	denotes	were
T6212	9213-9221	VBN	denotes	infected
T6213	9222-9226	IN	denotes	with
T6214	9227-9231	NNP	denotes	HXB2
T6215	9232-9247	JJ	denotes	Env-pseudotyped
T6216	9248-9255	NNS	denotes	virions
T6217	9256-9257	-LRB-	denotes	(
T6218	9257-9260	CD	denotes	500
T6219	9261-9263	NN	denotes	ng
T6220	9264-9266	IN	denotes	of
T6221	9267-9270	CD	denotes	p24
T6222	9270-9271	-RRB-	denotes	)
T6223	9272-9274	IN	denotes	by
T6224	9275-9288	NN	denotes	spinoculation
T6225	9288-9289	,	denotes	,
T6226	9290-9292	IN	denotes	as
T6227	9293-9303	RB	denotes	previously
T6228	9304-9313	VBN	denotes	described
T6229	9314-9315	-LRB-	denotes	(
T6230	9315-9317	CD	denotes	50
T6231	9317-9318	-RRB-	denotes	)
T6232	9318-9319	.	denotes	.
T6307	9321-9325	NN	denotes	Cell
T6308	9326-9334	NNS	denotes	extracts
T6309	9334-9335	,	denotes	,
T6310	9336-9343	JJ	denotes	western
T6311	9344-9352	VBG	denotes	blotting
T6312	9352-9353	,	denotes	,
T6313	9354-9357	NNP	denotes	IKK
T6314	9358-9366	NN	denotes	activity
T6315	9367-9370	CC	denotes	and
T6316	9371-9376	NNP	denotes	NF-κB
T6317	9377-9380	NNP	denotes	DNA
T6318	9381-9388	JJ	denotes	binding
T6319	9389-9394	NNP	denotes	Total
T6320	9394-9395	,	denotes	,
T6321	9396-9403	JJ	denotes	nuclear
T6322	9404-9407	CC	denotes	and
T6323	9408-9417	JJ	denotes	cytosolic
T6324	9418-9426	NNS	denotes	extracts
T6325	9427-9431	VBD	denotes	were
T6326	9432-9441	VBN	denotes	performed
T6327	9442-9444	IN	denotes	as
T6328	9445-9455	RB	denotes	previously
T6329	9456-9465	VBN	denotes	described
T6330	9466-9467	-LRB-	denotes	(
T6331	9467-9469	CD	denotes	54
T6332	9469-9470	-RRB-	denotes	)
T6333	9470-9471	:	denotes	;
T6334	9472-9479	NNS	denotes	details
T6335	9480-9483	VBP	denotes	are
T6336	9484-9492	VBN	denotes	reported
T6337	9493-9495	IN	denotes	in
T6338	9496-9509	NNP	denotes	Supplementary
T6339	9510-9514	NNP	denotes	Data
T6340	9514-9515	.	denotes	.
T6341	9516-9523	JJ	denotes	Western
T6342	9524-9532	VBG	denotes	blotting
T6343	9533-9541	NN	denotes	analysis
T6344	9542-9545	VBD	denotes	was
T6345	9546-9555	VBN	denotes	performed
T6346	9556-9558	IN	denotes	by
T6347	9559-9571	VBG	denotes	resuspending
T6348	9572-9579	NN	denotes	protein
T6349	9580-9588	NNS	denotes	aliquots
T6350	9589-9591	IN	denotes	in
T6351	9592-9599	VBG	denotes	loading
T6352	9600-9606	NN	denotes	buffer
T6353	9607-9608	-LRB-	denotes	(
T6354	9608-9611	CD	denotes	125
T6355	9612-9614	NNP	denotes	mM
T6356	9615-9619	NNP	denotes	Tris
T6357	9620-9623	NNP	denotes	HCl
T6358	9623-9624	,	denotes	,
T6359	9625-9627	NNP	denotes	pH
T6360	9628-9631	CD	denotes	6.8
T6361	9631-9632	,	denotes	,
T6362	9633-9634	CD	denotes	5
T6363	9634-9635	NN	denotes	%
T6364	9636-9639	NNP	denotes	SDS
T6365	9639-9640	,	denotes	,
T6366	9641-9642	CD	denotes	1
T6367	9642-9643	NN	denotes	%
T6368	9644-9655	NN	denotes	bromophenol
T6369	9656-9660	JJ	denotes	blue
T6370	9660-9661	,	denotes	,
T6371	9662-9664	CD	denotes	10
T6372	9664-9665	NN	denotes	%
T6373	9666-9683	NN	denotes	β-mercaptoethanol
T6374	9683-9684	,	denotes	,
T6375	9685-9687	CD	denotes	25
T6376	9687-9688	NN	denotes	%
T6377	9689-9697	NN	denotes	glycerol
T6378	9697-9698	-RRB-	denotes	)
T6379	9698-9699	,	denotes	,
T6380	9700-9708	VBN	denotes	resolved
T6381	9709-9711	IN	denotes	on
T6382	9712-9714	CD	denotes	12
T6383	9714-9715	NN	denotes	%
T6384	9716-9719	NNP	denotes	SDS
T6385	9720-9724	NNP	denotes	PAGE
T6386	9724-9725	,	denotes	,
T6387	9726-9737	VBD	denotes	transferred
T6388	9738-9740	TO	denotes	to
T6389	9741-9755	VB	denotes	polyvinylidene
T6390	9756-9766	NN	denotes	difluoride
T6391	9767-9775	NN	denotes	membrane
T6392	9776-9777	-LRB-	denotes	(
T6393	9777-9786	NNP	denotes	Millipore
T6394	9786-9787	,	denotes	,
T6395	9788-9795	NNP	denotes	Bedford
T6396	9795-9796	,	denotes	,
T6397	9797-9799	NNP	denotes	MA
T6398	9799-9800	,	denotes	,
T6399	9801-9804	NNP	denotes	USA
T6400	9804-9805	-RRB-	denotes	)
T6401	9806-9809	CC	denotes	and
T6402	9810-9819	VBN	denotes	incubated
T6403	9820-9824	IN	denotes	with
T6404	9825-9832	JJ	denotes	primary
T6405	9833-9843	NNS	denotes	antibodies
T6406	9844-9845	-LRB-	denotes	(
T6407	9845-9851	CD	denotes	1:1000
T6408	9851-9852	-RRB-	denotes	)
T6409	9853-9861	VBN	denotes	followed
T6410	9862-9864	IN	denotes	by
T6411	9865-9875	NN	denotes	incubation
T6412	9876-9880	IN	denotes	with
T6413	9881-9910	JJ	denotes	horseradish-peroxidase-linked
T6414	9911-9916	NN	denotes	mouse
T6415	9917-9919	CC	denotes	or
T6416	9920-9926	NN	denotes	rabbit
T6417	9927-9930	NNP	denotes	IgG
T6418	9931-9932	-LRB-	denotes	(
T6419	9932-9938	CD	denotes	1:2000
T6420	9938-9939	-RRB-	denotes	)
T6421	9940-9941	-LRB-	denotes	(
T6422	9941-9943	NNP	denotes	GE
T6423	9944-9954	NNP	denotes	Healthcare
T6424	9955-9963	NNP	denotes	Amersham
T6425	9963-9964	,	denotes	,
T6426	9965-9971	NNP	denotes	Little
T6427	9972-9980	NNP	denotes	Chalfont
T6428	9980-9981	,	denotes	,
T6429	9982-9997	NNP	denotes	Buckinghamshire
T6430	9997-9998	,	denotes	,
T6431	9999-10001	NNP	denotes	UK
T6432	10001-10002	-RRB-	denotes	)
T6433	10003-10005	IN	denotes	in
T6434	10006-10009	NNP	denotes	PBS
T6435	10010-10020	VBG	denotes	containing
T6436	10021-10022	CD	denotes	5
T6437	10022-10023	NN	denotes	%
T6438	10024-10031	JJ	denotes	non-fat
T6439	10032-10035	JJ	denotes	dry
T6440	10036-10040	NN	denotes	milk
T6441	10041-10042	-LRB-	denotes	(
T6442	10042-10049	NNP	denotes	Bio-Rad
T6443	10050-10062	NNPS	denotes	Laboratories
T6444	10062-10063	-RRB-	denotes	)
T6445	10063-10064	.	denotes	.
T6446	10065-10073	NNPS	denotes	Proteins
T6447	10074-10078	VBD	denotes	were
T6448	10079-10087	VBN	denotes	detected
T6449	10088-10090	IN	denotes	by
T6450	10091-10108	NN	denotes	chemiluminescence
T6451	10109-10114	VBG	denotes	using
T6452	10115-10118	DT	denotes	the
T6453	10119-10122	NNP	denotes	ECL
T6454	10123-10129	NNP	denotes	System
T6455	10130-10131	-LRB-	denotes	(
T6456	10131-10133	NNP	denotes	GE
T6457	10134-10144	NNP	denotes	Healthcare
T6458	10145-10153	NNP	denotes	Amersham
T6459	10153-10154	-RRB-	denotes	)
T6460	10154-10155	.	denotes	.
T6461	10156-10163	JJ	denotes	Primary
T6462	10164-10174	NNS	denotes	antibodies
T6463	10175-10179	VBD	denotes	were
T6464	10180-10189	VBN	denotes	purchased
T6465	10190-10194	IN	denotes	from
T6466	10194-10195	:	denotes	:
T6467	10196-10201	NNP	denotes	Santa
T6468	10202-10206	NNP	denotes	Cruz
T6469	10207-10220	NNP	denotes	Biotechnology
T6470	10220-10221	,	denotes	,
T6471	10222-10227	NNP	denotes	Santa
T6472	10228-10232	NNP	denotes	Cruz
T6473	10232-10233	,	denotes	,
T6474	10234-10236	NNP	denotes	CA
T6475	10236-10237	,	denotes	,
T6476	10238-10241	NNP	denotes	USA
T6477	10242-10243	-LRB-	denotes	(
T6478	10243-10250	JJ	denotes	anti-HA
T6479	10251-10253	NNP	denotes	F7
T6480	10253-10254	,	denotes	,
T6481	10255-10265	NNP	denotes	anti-IκB-α
T6482	10266-10269	NNP	denotes	C15
T6483	10269-10270	,	denotes	,
T6484	10271-10283	JJ	denotes	anti-Histone
T6485	10284-10286	NNP	denotes	H1
T6486	10286-10287	,	denotes	,
T6487	10288-10306	NNP	denotes	anti-Hexokinase-II
T6488	10306-10307	-RRB-	denotes	)
T6489	10307-10308	:	denotes	;
T6490	10309-10322	NNP	denotes	Sigma-Aldrich
T6491	10323-10324	-LRB-	denotes	(
T6492	10324-10333	JJ	denotes	anti-FLAG
T6493	10334-10336	NN	denotes	M2
T6494	10336-10337	,	denotes	,
T6495	10338-10352	JJ	denotes	anti-γ-Tubulin
T6496	10352-10353	-RRB-	denotes	)
T6497	10353-10354	:	denotes	;
T6498	10355-10362	NNP	denotes	Upstate
T6499	10362-10363	,	denotes	,
T6500	10364-10368	NNP	denotes	Lake
T6501	10369-10375	NNP	denotes	Placid
T6502	10375-10376	,	denotes	,
T6503	10377-10379	NNP	denotes	NY
T6504	10379-10380	,	denotes	,
T6505	10381-10384	NNP	denotes	USA
T6506	10385-10386	-LRB-	denotes	(
T6507	10386-10394	JJ	denotes	anti-p65
T6508	10394-10395	-RRB-	denotes	)
T6509	10395-10396	.	denotes	.
T6510	10397-10409	NNP	denotes	Densitometry
T6511	10410-10412	IN	denotes	of
T6512	10413-10419	JJ	denotes	single
T6513	10420-10425	NNS	denotes	bands
T6514	10426-10429	VBD	denotes	was
T6515	10430-10438	VBN	denotes	analysed
T6516	10439-10441	IN	denotes	by
T6517	10442-10448	NNP	denotes	ImageJ
T6518	10449-10457	NN	denotes	software
T6519	10458-10465	NN	denotes	package
T6520	10466-10467	-LRB-	denotes	(
T6521	10467-10470	NNP	denotes	NIH
T6522	10470-10471	,	denotes	,
T6523	10472-10475	NNP	denotes	USA
T6524	10475-10476	-RRB-	denotes	)
T6525	10476-10477	.	denotes	.
T6526	10478-10481	NNP	denotes	IKK
T6527	10482-10490	NN	denotes	activity
T6528	10491-10494	VBD	denotes	was
T6529	10495-10504	VBN	denotes	evaluated
T6530	10505-10507	IN	denotes	in
T6531	10508-10517	JJ	denotes	cytosolic
T6532	10518-10526	NNS	denotes	extracts
T6533	10527-10532	VBG	denotes	using
T6534	10533-10536	DT	denotes	the
T6535	10537-10543	NNP	denotes	HTScan
T6536	10544-10547	NNP	denotes	IKK
T6537	10548-10554	NN	denotes	kinase
T6538	10555-10560	NN	denotes	assay
T6539	10561-10564	NN	denotes	kit
T6540	10565-10566	-LRB-	denotes	(
T6541	10566-10570	NNP	denotes	Cell
T6542	10571-10580	NNP	denotes	Signaling
T6543	10581-10591	NNP	denotes	Technology
T6544	10591-10592	,	denotes	,
T6545	10593-10600	NNP	denotes	Danvers
T6546	10600-10601	,	denotes	,
T6547	10602-10604	NNP	denotes	MA
T6548	10604-10605	,	denotes	,
T6549	10606-10609	NNP	denotes	USA
T6550	10609-10610	-RRB-	denotes	)
T6551	10610-10611	.	denotes	.
T6552	10612-10619	VBG	denotes	Binding
T6553	10620-10622	IN	denotes	of
T6554	10623-10626	CD	denotes	p65
T6555	10626-10627	,	denotes	,
T6556	10628-10631	CD	denotes	p50
T6557	10632-10635	CC	denotes	and
T6558	10636-10644	NNP	denotes	FLAG-Tat
T6559	10645-10647	TO	denotes	to
T6560	10648-10651	DT	denotes	the
T6561	10652-10667	JJ	denotes	double-stranded
T6562	10668-10673	NN	denotes	NF-κB
T6563	10674-10689	NN	denotes	oligonucleotide
T6564	10690-10693	VBD	denotes	was
T6565	10694-10702	VBN	denotes	measured
T6566	10703-10708	VBG	denotes	using
T6567	10709-10714	NNP	denotes	NF-κB
T6568	10715-10720	NNP	denotes	Combo
T6569	10721-10734	NNP	denotes	Transcription
T6570	10735-10741	NNP	denotes	Factor
T6571	10742-10747	NNP	denotes	Assay
T6572	10748-10751	NN	denotes	kit
T6573	10752-10753	-LRB-	denotes	(
T6574	10753-10759	NNP	denotes	Cayman
T6575	10760-10768	NNP	denotes	Chemical
T6576	10769-10776	NNP	denotes	Company
T6577	10776-10777	,	denotes	,
T6578	10778-10781	NNP	denotes	Ann
T6579	10782-10787	NNP	denotes	Arbor
T6580	10787-10788	,	denotes	,
T6581	10789-10791	NNP	denotes	MI
T6582	10791-10792	,	denotes	,
T6583	10793-10796	NNP	denotes	USA
T6584	10796-10797	-RRB-	denotes	)
T6585	10797-10798	.	denotes	.
T6586	10799-10814	NNP	denotes	Electrophoretic
T6587	10815-10823	NNP	denotes	Mobility
T6588	10824-10829	NNP	denotes	Shift
T6589	10830-10835	NNP	denotes	Assay
T6590	10836-10837	-LRB-	denotes	(
T6591	10837-10841	NNP	denotes	EMSA
T6592	10841-10842	-RRB-	denotes	)
T6593	10843-10846	VBD	denotes	was
T6594	10847-10856	VBN	denotes	performed
T6595	10857-10859	IN	denotes	as
T6596	10860-10870	RB	denotes	previously
T6597	10871-10880	VBN	denotes	described
T6598	10881-10882	-LRB-	denotes	(
T6599	10882-10884	CD	denotes	55
T6600	10884-10885	-RRB-	denotes	)
T6601	10885-10886	:	denotes	;
T6602	10887-10894	NNS	denotes	details
T6603	10895-10898	VBP	denotes	are
T6604	10899-10908	VBN	denotes	described
T6605	10909-10911	IN	denotes	in
T6606	10912-10925	NNP	denotes	Supplementary
T6607	10926-10930	NNP	denotes	Data
T6608	10930-10931	.	denotes	.
T6765	10933-10935	IN	denotes	In
T6766	10936-10941	NN	denotes	vitro
T6767	10942-10953	NN	denotes	translation
T6768	10954-10962	NNP	denotes	HA-IκB-α
T6769	10962-10963	,	denotes	,
T6770	10964-10967	CD	denotes	p65
T6771	10968-10971	CC	denotes	and
T6772	10972-10975	NNP	denotes	Tat
T6773	10976-10980	VBD	denotes	were
T6774	10981-10990	VBN	denotes	expressed
T6775	10991-10996	IN	denotes	under
T6776	10997-11000	DT	denotes	the
T6777	11001-11003	CD	denotes	T7
T6778	11004-11012	NN	denotes	promoter
T6779	11013-11016	CC	denotes	and
T6780	11017-11019	IN	denotes	in
T6781	11020-11025	NN	denotes	vitro
T6782	11026-11036	VBN	denotes	translated
T6783	11037-11042	VBG	denotes	using
T6784	11043-11046	DT	denotes	the
T6785	11047-11050	NNP	denotes	TnT
T6786	11051-11056	JJ	denotes	quick
T6787	11057-11064	VBN	denotes	coupled
T6788	11065-11090	NN	denotes	transcription/translation
T6789	11091-11097	NN	denotes	system
T6790	11098-11099	-LRB-	denotes	(
T6791	11099-11106	NNP	denotes	Promega
T6792	11106-11107	-RRB-	denotes	)
T6793	11107-11108	,	denotes	,
T6794	11109-11111	IN	denotes	as
T6795	11112-11122	RB	denotes	previously
T6796	11123-11131	VBN	denotes	reported
T6797	11132-11133	-LRB-	denotes	(
T6798	11133-11135	CD	denotes	50
T6799	11135-11136	-RRB-	denotes	)
T6800	11136-11137	.	denotes	.
T6801	11138-11145	NNS	denotes	Details
T6802	11146-11149	VBP	denotes	are
T6803	11150-11159	VBN	denotes	described
T6804	11160-11162	IN	denotes	in
T6805	11163-11176	NNP	denotes	Supplementary
T6806	11177-11181	NNP	denotes	Data
T6807	11181-11182	.	denotes	.
T6916	11184-11203	NNP	denotes	Immunoprecipitation
T6917	11204-11209	NN	denotes	assay
T6918	11210-11213	CC	denotes	and
T6919	11214-11222	NNP	denotes	GST-pull
T6920	11223-11227	IN	denotes	down
T6921	11228-11247	NNP	denotes	Immunoprecipitation
T6922	11247-11248	,	denotes	,
T6923	11249-11257	NNP	denotes	GST-pull
T6924	11258-11262	RB	denotes	down
T6925	11262-11263	,	denotes	,
T6926	11264-11267	CC	denotes	and
T6927	11268-11278	NN	denotes	production
T6928	11279-11281	IN	denotes	of
T6929	11282-11285	NNP	denotes	GST
T6930	11286-11294	NNS	denotes	proteins
T6931	11295-11297	IN	denotes	in
T6932	11298-11309	NNP	denotes	Escherichia
T6933	11310-11314	NNS	denotes	coli
T6934	11315-11321	VBP	denotes	strain
T6935	11322-11326	CD	denotes	BL21
T6936	11327-11331	VBD	denotes	were
T6937	11332-11341	VBN	denotes	performed
T6938	11342-11344	IN	denotes	as
T6939	11345-11355	RB	denotes	previously
T6940	11356-11364	VBN	denotes	reported
T6941	11365-11366	-LRB-	denotes	(
T6942	11366-11368	CD	denotes	50
T6943	11368-11369	-RRB-	denotes	)
T6944	11369-11370	.	denotes	.
T6945	11371-11378	NNS	denotes	Details
T6946	11379-11382	VBP	denotes	are
T6947	11383-11392	VBN	denotes	described
T6948	11393-11395	IN	denotes	in
T6949	11396-11409	NNP	denotes	Supplementary
T6950	11410-11414	NNP	denotes	Data
T6951	11414-11415	.	denotes	.
T7201	11417-11426	JJ	denotes	Real-time
T7202	11427-11430	NNP	denotes	PCR
T7203	11431-11436	NNP	denotes	Total
T7204	11437-11440	NNP	denotes	RNA
T7205	11441-11444	VBD	denotes	was
T7206	11445-11454	VBN	denotes	extracted
T7207	11455-11459	IN	denotes	from
T7208	11460-11465	NNS	denotes	cells
T7209	11466-11468	IN	denotes	by
T7210	11469-11474	VBG	denotes	using
T7211	11475-11478	DT	denotes	the
T7212	11479-11485	NNP	denotes	TRIzol
T7213	11486-11493	NN	denotes	reagent
T7214	11494-11495	-LRB-	denotes	(
T7215	11495-11505	NNP	denotes	Invitrogen
T7216	11505-11506	-RRB-	denotes	)
T7217	11506-11507	:	denotes	;
T7218	11508-11511	NNP	denotes	RNA
T7219	11512-11520	NNS	denotes	aliquots
T7220	11521-11522	-LRB-	denotes	(
T7221	11522-11525	CD	denotes	200
T7222	11526-11528	NN	denotes	ng
T7223	11528-11529	-RRB-	denotes	)
T7224	11530-11534	VBD	denotes	were
T7225	11535-11542	RB	denotes	reverse
T7226	11543-11554	VBN	denotes	transcribed
T7227	11555-11560	VBG	denotes	using
T7228	11561-11567	NNP	denotes	Random
T7229	11568-11575	NNP	denotes	Examers
T7230	11576-11577	-LRB-	denotes	(
T7231	11577-11582	NNP	denotes	Roche
T7232	11582-11583	-RRB-	denotes	)
T7233	11584-11587	CC	denotes	and
T7234	11588-11599	NNP	denotes	Superscript
T7235	11600-11603	NNP	denotes	III
T7236	11604-11611	VB	denotes	Reverse
T7237	11612-11625	NNP	denotes	Transcriptase
T7238	11626-11627	-LRB-	denotes	(
T7239	11627-11637	NNP	denotes	Invitrogen
T7240	11637-11638	-RRB-	denotes	)
T7241	11638-11639	,	denotes	,
T7242	11640-11649	VBG	denotes	according
T7243	11650-11652	TO	denotes	to
T7244	11653-11656	DT	denotes	the
T7245	11657-11669	NN	denotes	manufacturer
T7246	11669-11671	POS	denotes	's
T7247	11672-11680	NN	denotes	protocol
T7248	11680-11681	.	denotes	.
T7249	11682-11691	JJ	denotes	Real-time
T7250	11692-11695	NNP	denotes	PCR
T7251	11696-11699	VBD	denotes	was
T7252	11700-11709	VBN	denotes	performed
T7253	11710-11714	IN	denotes	with
T7254	11715-11718	DT	denotes	the
T7255	11719-11721	NNP	denotes	iQ
T7256	11722-11727	NNP	denotes	Green
T7257	11728-11733	NNP	denotes	Super
T7258	11734-11737	NN	denotes	mix
T7259	11738-11739	-LRB-	denotes	(
T7260	11739-11746	NNP	denotes	Bio-Rad
T7261	11747-11759	NNPS	denotes	Laboratories
T7262	11759-11760	-RRB-	denotes	)
T7263	11761-11764	CC	denotes	and
T7264	11765-11772	VBD	denotes	carried
T7265	11773-11776	RP	denotes	out
T7266	11777-11781	IN	denotes	with
T7267	11782-11785	DT	denotes	the
T7268	11786-11793	NNP	denotes	iCycler
T7269	11794-11796	NNP	denotes	iQ
T7270	11797-11806	NNP	denotes	Real-Time
T7271	11807-11816	NN	denotes	detection
T7272	11817-11823	NN	denotes	system
T7273	11824-11825	-LRB-	denotes	(
T7274	11825-11832	NNP	denotes	Bio-Rad
T7275	11833-11845	NNPS	denotes	Laboratories
T7276	11845-11846	-RRB-	denotes	)
T7277	11847-11852	IN	denotes	under
T7278	11853-11856	DT	denotes	the
T7279	11857-11866	JJ	denotes	following
T7280	11867-11877	NNS	denotes	conditions
T7281	11877-11878	:	denotes	:
T7282	11879-11881	CD	denotes	95
T7283	11881-11882	NN	denotes	°
T7284	11882-11883	NNP	denotes	C
T7285	11883-11884	,	denotes	,
T7286	11885-11886	CD	denotes	1
T7287	11887-11890	NN	denotes	min
T7288	11890-11891	:	denotes	;
T7289	11892-11893	-LRB-	denotes	(
T7290	11893-11895	CD	denotes	94
T7291	11895-11896	NN	denotes	°
T7292	11896-11897	NNP	denotes	C
T7293	11897-11898	,	denotes	,
T7294	11899-11901	CD	denotes	10
T7295	11902-11903	PRP	denotes	s
T7296	11903-11904	:	denotes	;
T7297	11905-11907	CD	denotes	60
T7298	11907-11908	NN	denotes	°
T7299	11908-11909	NNP	denotes	C
T7300	11909-11910	,	denotes	,
T7301	11911-11913	CD	denotes	30
T7302	11914-11915	PRP	denotes	s
T7303	11915-11916	-RRB-	denotes	)
T7304	11917-11918	RB	denotes	×
T7305	11918-11920	CD	denotes	40
T7306	11920-11921	.	denotes	.
T7307	11922-11929	NNS	denotes	Primers
T7308	11930-11933	IN	denotes	for
T7309	11934-11937	NNP	denotes	Tat
T7310	11938-11941	CC	denotes	and
T7311	11942-11948	NNP	denotes	MIP-1α
T7312	11949-11952	VBP	denotes	are
T7313	11953-11959	VBN	denotes	listed
T7314	11960-11962	IN	denotes	in
T7315	11963-11976	NNP	denotes	Supplementary
T7316	11977-11981	NNP	denotes	Data
T7317	11981-11982	.	denotes	.
T7318	11983-11992	JJ	denotes	Real-time
T7319	11993-11996	NNP	denotes	PCR
T7320	11997-11999	IN	denotes	of
T7321	12000-12004	NNP	denotes	CSF3
T7322	12004-12005	,	denotes	,
T7323	12006-12009	NNP	denotes	LTA
T7324	12009-12010	,	denotes	,
T7325	12011-12017	NNP	denotes	NFKBIA
T7326	12017-12018	,	denotes	,
T7327	12019-12023	NNP	denotes	TLR2
T7328	12023-12024	,	denotes	,
T7329	12025-12030	NNP	denotes	GAPDH
T7330	12031-12034	CC	denotes	and
T7331	12035-12039	NNP	denotes	ACTB
T7332	12040-12043	VBD	denotes	was
T7333	12044-12053	VBN	denotes	performed
T7334	12054-12059	VBG	denotes	using
T7335	12060-12063	DT	denotes	the
T7336	12064-12067	NNP	denotes	RT2
T7337	12068-12076	NN	denotes	profiler
T7338	12077-12080	NNP	denotes	PCR
T7339	12081-12092	NNP	denotes	Array-Human
T7340	12093-12098	NNP	denotes	NF-κB
T7341	12099-12108	VBG	denotes	signaling
T7342	12109-12116	NN	denotes	pathway
T7343	12117-12118	-LRB-	denotes	(
T7344	12118-12124	NNP	denotes	QIAGEN
T7345	12125-12133	NNPS	denotes	Sciences
T7346	12133-12134	,	denotes	,
T7347	12135-12137	NNP	denotes	MD
T7348	12137-12138	,	denotes	,
T7349	12139-12142	NNP	denotes	USA
T7350	12142-12143	-RRB-	denotes	)
T7351	12143-12144	.	denotes	.
T7352	12145-12154	NNS	denotes	Reactions
T7353	12155-12159	VBD	denotes	were
T7354	12160-12167	VBN	denotes	carried
T7355	12168-12171	RP	denotes	out
T7356	12172-12174	IN	denotes	in
T7357	12175-12185	NN	denotes	triplicate
T7358	12185-12186	,	denotes	,
T7359	12187-12190	CC	denotes	and
T7360	12191-12195	NN	denotes	gene
T7361	12196-12206	NN	denotes	expression
T7362	12207-12213	NNS	denotes	levels
T7363	12214-12218	VBD	denotes	were
T7364	12219-12229	VBN	denotes	calculated
T7365	12230-12238	JJ	denotes	relative
T7366	12239-12241	TO	denotes	to
T7367	12242-12247	NNP	denotes	GAPDH
T7368	12248-12252	NNP	denotes	mRNA
T7369	12253-12259	NNS	denotes	levels
T7370	12260-12262	IN	denotes	as
T7371	12263-12273	JJ	denotes	endogenous
T7372	12274-12281	NN	denotes	control
T7373	12281-12282	.	denotes	.
T7374	12283-12291	JJ	denotes	Relative
T7375	12292-12302	NN	denotes	expression
T7376	12303-12306	VBD	denotes	was
T7377	12307-12317	VBN	denotes	calculated
T7378	12318-12320	IN	denotes	as
T7379	12321-12322	CD	denotes	2
T7380	12322-12323	-LRB-	denotes	(
T7381	12323-12325	NN	denotes	Ct
T7382	12326-12330	NN	denotes	gene
T7383	12331-12336	IN	denotes	under
T7384	12337-12350	NN	denotes	investigation
T7385	12351-12352	NN	denotes	−
T7386	12353-12355	NNP	denotes	Ct
T7387	12356-12361	NNP	denotes	GAPDH
T7388	12361-12362	-RRB-	denotes	)
T7389	12362-12363	.	denotes	.
T7546	12365-12374	NNP	denotes	Chromatin
T7547	12375-12394	NN	denotes	immunoprecipitation
T7548	12395-12400	NN	denotes	assay
T7549	12401-12406	NNS	denotes	Cells
T7550	12407-12411	VBD	denotes	were
T7551	12412-12417	VBN	denotes	fixed
T7552	12418-12420	IN	denotes	by
T7553	12421-12427	VBG	denotes	adding
T7554	12428-12440	NN	denotes	formaldehyde
T7555	12441-12442	-LRB-	denotes	(
T7556	12442-12455	NNP	denotes	Sigma-Aldrich
T7589	12610-12613	NNP	denotes	PBS
T7590	12613-12614	,	denotes	,
T7591	12615-12618	CC	denotes	and
T7592	12619-12626	VBD	denotes	scraped
T7593	12626-12627	.	denotes	.
T7594	12628-12633	IN	denotes	After
T7595	12634-12648	NN	denotes	centrifugation
T7596	12648-12649	,	denotes	,
T7597	12650-12655	NNS	denotes	cells
T7598	12656-12660	VBD	denotes	were
T7599	12661-12663	CD	denotes	10
T7600	12664-12667	NN	denotes	min
T7601	12668-12673	VBN	denotes	lysed
T7602	12674-12676	IN	denotes	in
T7603	12677-12682	NN	denotes	lysis
T7604	12683-12689	NN	denotes	buffer
T7605	12690-12691	-LRB-	denotes	(
T7606	12691-12692	CD	denotes	5
T7607	12693-12695	NNP	denotes	mM
T7608	12696-12701	NNP	denotes	PIPES
T7609	12702-12704	NNP	denotes	pH
T7610	12705-12708	CD	denotes	8.0
T7611	12708-12709	,	denotes	,
T7612	12710-12712	CD	denotes	85
T7613	12713-12715	NNP	denotes	mM
T7614	12716-12719	NNP	denotes	KCl
T7615	12719-12720	,	denotes	,
T7616	12721-12724	CD	denotes	0.5
T7617	12724-12725	NN	denotes	%
T7618	12726-12731	NN	denotes	NP-40
T7619	12731-12732	-RRB-	denotes	)
T7620	12733-12745	VBN	denotes	supplemented
T7621	12746-12750	IN	denotes	with
T7622	12751-12752	CD	denotes	1
T7623	12752-12753	CD	denotes	×
T7624	12754-12762	JJ	denotes	Complete
T7625	12763-12771	NNP	denotes	Protease
T7626	12772-12781	NNP	denotes	Inhibitor
T7627	12782-12783	-LRB-	denotes	(
T7628	12783-12788	NNP	denotes	Roche
T7629	12789-12799	NNP	denotes	Diagnostic
T7630	12800-12804	NNP	denotes	GmbH
T7631	12804-12805	-RRB-	denotes	)
T7632	12805-12806	.	denotes	.
T7633	12807-12813	NNP	denotes	Nuclei
T7634	12814-12818	VBD	denotes	were
T7635	12819-12828	VBN	denotes	pelletted
T7636	12829-12830	-LRB-	denotes	(
T7637	12830-12834	CD	denotes	1000
T7638	12835-12836	NN	denotes	×
T7639	12837-12838	NN	denotes	g
T7640	12838-12839	,	denotes	,
T7641	12840-12841	CD	denotes	5
T7642	12842-12845	NN	denotes	min
T7643	12845-12846	-RRB-	denotes	)
T7644	12846-12847	,	denotes	,
T7645	12848-12851	CC	denotes	and
T7646	12852-12863	VBD	denotes	resuspended
T7647	12864-12866	IN	denotes	in
T7648	12867-12877	NN	denotes	sonication
T7649	12878-12884	NN	denotes	buffer
T7650	12885-12886	-LRB-	denotes	(
T7651	12886-12888	CD	denotes	50
T7652	12889-12891	NNP	denotes	mM
T7653	12892-12896	NNP	denotes	Tris
T7654	12897-12900	NNP	denotes	HCl
T7655	12901-12903	NNP	denotes	pH
T7656	12904-12907	CD	denotes	8.0
T7657	12907-12908	,	denotes	,
T7658	12909-12910	CD	denotes	1
T7659	12910-12911	NN	denotes	%
T7660	12912-12915	NNP	denotes	SDS
T7661	12915-12916	,	denotes	,
T7662	12917-12919	CD	denotes	10
T7663	12920-12922	NNP	denotes	mM
T7664	12923-12927	NNP	denotes	EDTA
T7665	12927-12928	-RRB-	denotes	)
T7666	12928-12929	.	denotes	.
T7667	12930-12939	NNP	denotes	Chromatin
T7668	12940-12943	VBD	denotes	was
T7669	12944-12953	VBN	denotes	sonicated
T7670	12954-12959	VBG	denotes	using
T7671	12960-12968	NNP	denotes	Bandelin
T7672	12969-12977	NNPS	denotes	Sonoplus
T7673	12978-12982	NNP	denotes	GM70
T7674	12983-12984	-LRB-	denotes	(
T7675	12984-12992	NNP	denotes	Bandelin
T7676	12993-13003	NNP	denotes	Electronic
T7677	13003-13004	,	denotes	,
T7678	13005-13011	NNP	denotes	Berlin
T7679	13011-13012	,	denotes	,
T7680	13013-13020	NNP	denotes	Germany
T7681	13020-13021	-RRB-	denotes	)
T7682	13021-13022	,	denotes	,
T7683	13023-13034	VBN	denotes	centrifuged
T7684	13035-13036	-LRB-	denotes	(
T7685	13036-13038	CD	denotes	14
T7686	13039-13042	CD	denotes	000
T7687	13043-13044	NN	denotes	×
T7688	13045-13046	NN	denotes	g
T7689	13046-13047	,	denotes	,
T7690	13048-13050	CD	denotes	15
T7691	13051-13054	NN	denotes	min
T7692	13054-13055	-RRB-	denotes	)
T7693	13055-13056	,	denotes	,
T7694	13057-13060	CC	denotes	and
T7695	13061-13072	NN	denotes	supernatant
T7696	13073-13076	VBD	denotes	was
T7697	13077-13084	JJ	denotes	10-fold
T7698	13085-13092	VBN	denotes	diluted
T7699	13093-13095	IN	denotes	in
T7700	13096-13104	NN	denotes	dilution
T7701	13105-13111	NN	denotes	buffer
T7702	13112-13113	-LRB-	denotes	(
T7703	13113-13117	CD	denotes	0.01
T7704	13117-13118	NN	denotes	%
T7705	13119-13122	NNP	denotes	SDS
T7706	13122-13123	,	denotes	,
T7707	13124-13128	CD	denotes	16.7
T7708	13129-13131	NNP	denotes	mM
T7709	13132-13136	NNP	denotes	Tris
T7710	13137-13140	NNP	denotes	HCl
T7711	13141-13143	NNP	denotes	pH
T7712	13144-13147	CD	denotes	8.0
T7713	13147-13148	,	denotes	,
T7714	13149-13152	CD	denotes	1.1
T7715	13152-13153	NN	denotes	%
T7716	13154-13160	NNP	denotes	Triton
T7717	13161-13166	NNP	denotes	X-100
T7718	13166-13167	,	denotes	,
T7719	13168-13171	CD	denotes	167
T7720	13172-13174	NNP	denotes	mM
T7721	13175-13179	NNP	denotes	NaCl
T7722	13179-13180	,	denotes	,
T7723	13181-13184	CD	denotes	1.2
T7724	13185-13187	NNP	denotes	mM
T7725	13188-13192	NNP	denotes	EDTA
T7726	13192-13193	-RRB-	denotes	)
T7727	13193-13194	.	denotes	.
T7728	13195-13202	NNS	denotes	Samples
T7729	13203-13207	VBD	denotes	were
T7730	13208-13219	JJ	denotes	pre-cleared
T7731	13220-13222	IN	denotes	by
T7732	13223-13226	JJ	denotes	3-h
T7733	13227-13237	NN	denotes	incubation
T7734	13238-13242	IN	denotes	with
T7735	13243-13245	CD	denotes	20
T7736	13246-13248	NN	denotes	µl
T7737	13249-13251	IN	denotes	of
T7738	13252-13259	NN	denotes	protein
T7739	13260-13261	NNP	denotes	G
T7740	13262-13269	JJ	denotes	agarose
T7741	13270-13275	NNS	denotes	beads
T7742	13276-13284	VBN	denotes	followed
T7743	13285-13287	IN	denotes	by
T7744	13288-13298	NN	denotes	incubation
T7745	13299-13303	IN	denotes	with
T7746	13304-13314	NNS	denotes	antibodies
T7747	13315-13322	IN	denotes	against
T7748	13323-13326	DT	denotes	the
T7749	13327-13335	JJ	denotes	analysed
T7750	13336-13344	NNS	denotes	proteins
T7751	13344-13345	.	denotes	.
T7752	13346-13353	JJ	denotes	Primary
T7753	13354-13364	NNS	denotes	antibodies
T7754	13365-13369	VBD	denotes	were
T7755	13369-13370	:	denotes	:
T7756	13371-13379	JJ	denotes	anti-p65
T7757	13380-13381	-LRB-	denotes	(
T7758	13381-13387	JJ	denotes	sc-372
T7759	13387-13388	-RRB-	denotes	)
T7760	13388-13389	,	denotes	,
T7761	13390-13399	JJ	denotes	anti-IκBα
T7762	13400-13401	-LRB-	denotes	(
T7763	13401-13407	JJ	denotes	sc-203
T7764	13407-13408	-RRB-	denotes	)
T7765	13409-13412	CC	denotes	and
T7766	13413-13419	NN	denotes	rabbit
T7767	13420-13423	NNP	denotes	IgG
T7768	13424-13425	-LRB-	denotes	(
T7769	13425-13432	JJ	denotes	sc-2027
T7770	13432-13433	-RRB-	denotes	)
T7771	13434-13438	IN	denotes	from
T7772	13439-13444	NNP	denotes	Santa
T7773	13445-13449	NNP	denotes	Cruz
T7774	13450-13463	NNP	denotes	Biotechnology
T7775	13463-13464	:	denotes	;
T7776	13465-13474	NNP	denotes	anti-FLAG
T7777	13475-13477	NNP	denotes	M2
T7778	13478-13482	IN	denotes	from
T7779	13483-13488	NNP	denotes	Sigma
T7780	13489-13496	NNP	denotes	Aldrich
T7781	13496-13497	.	denotes	.
T7782	13498-13518	NNS	denotes	Immunoprecipitations
T7783	13519-13523	VBD	denotes	were
T7784	13524-13531	VBN	denotes	carried
T7785	13532-13535	RP	denotes	out
T7786	13536-13538	IN	denotes	at
T7787	13539-13540	CD	denotes	4
T7788	13540-13541	CD	denotes	°
T7789	13541-13542	NNP	denotes	C
T7790	13543-13552	JJ	denotes	overnight
T7791	13553-13556	CC	denotes	and
T7792	13557-13563	JJ	denotes	immune
T7793	13564-13573	NNS	denotes	complexes
T7794	13574-13578	VBD	denotes	were
T7795	13579-13588	VBN	denotes	collected
T7796	13589-13593	IN	denotes	with
T7797	13594-13601	NN	denotes	protein
T7798	13602-13603	NNP	denotes	G
T7799	13604-13611	JJ	denotes	agarose
T7800	13612-13617	NNS	denotes	beads
T7801	13617-13618	,	denotes	,
T7802	13619-13625	VBD	denotes	washed
T7803	13626-13630	CD	denotes	five
T7804	13631-13636	NNS	denotes	times
T7805	13637-13641	IN	denotes	with
T7806	13642-13645	JJ	denotes	low
T7807	13646-13650	NN	denotes	salt
T7808	13651-13657	NN	denotes	buffer
T7809	13658-13659	-LRB-	denotes	(
T7810	13659-13661	CD	denotes	20
T7811	13662-13664	NNP	denotes	mM
T7812	13665-13669	NNP	denotes	Tris
T7813	13670-13673	NNP	denotes	HCl
T7814	13674-13676	NNP	denotes	pH
T7815	13677-13680	CD	denotes	8.0
T7816	13680-13681	,	denotes	,
T7817	13682-13685	CD	denotes	0.1
T7818	13685-13686	NN	denotes	%
T7819	13687-13690	NNP	denotes	SDS
T7820	13690-13691	,	denotes	,
T7821	13692-13693	CD	denotes	1
T7822	13693-13694	NN	denotes	%
T7823	13695-13701	NNP	denotes	Triton
T7824	13702-13707	NNP	denotes	X-100
T7825	13707-13708	,	denotes	,
T7826	13709-13710	CD	denotes	2
T7827	13711-13713	NNP	denotes	mM
T7828	13714-13718	NNP	denotes	EDTA
T7829	13718-13719	,	denotes	,
T7830	13720-13723	CD	denotes	150
T7831	13724-13726	NNP	denotes	mM
T7832	13727-13731	NNP	denotes	NaCl
T7833	13731-13732	-RRB-	denotes	)
T7834	13732-13733	,	denotes	,
T7835	13734-13738	CD	denotes	four
T7836	13739-13744	NNS	denotes	times
T7837	13745-13749	IN	denotes	with
T7838	13750-13754	JJ	denotes	high
T7839	13755-13759	NN	denotes	salt
T7840	13760-13766	NN	denotes	buffer
T7841	13767-13768	-LRB-	denotes	(
T7842	13768-13770	CD	denotes	20
T7843	13771-13773	NNP	denotes	mM
T7844	13774-13778	NNP	denotes	Tris
T7845	13779-13782	NNP	denotes	HCl
T7846	13783-13785	NNP	denotes	pH
T7847	13786-13789	CD	denotes	8.0
T7848	13789-13790	,	denotes	,
T7849	13791-13794	CD	denotes	0.1
T7850	13794-13795	NN	denotes	%
T7851	13796-13799	NNP	denotes	SDS
T7852	13799-13800	,	denotes	,
T7853	13801-13802	CD	denotes	1
T7854	13802-13803	NN	denotes	%
T7855	13804-13810	NNP	denotes	Triton
T7856	13811-13816	NNP	denotes	X-100
T7857	13816-13817	,	denotes	,
T7858	13818-13819	CD	denotes	2
T7859	13820-13822	NNP	denotes	mM
T7860	13823-13827	NNP	denotes	EDTA
T7861	13827-13828	,	denotes	,
T7862	13829-13832	CD	denotes	500
T7863	13833-13835	NNP	denotes	mM
T7864	13836-13840	NNP	denotes	NaCl
T7865	13840-13841	-RRB-	denotes	)
T7866	13841-13842	,	denotes	,
T7867	13843-13847	RB	denotes	once
T7868	13848-13852	IN	denotes	with
T7869	13853-13855	NNP	denotes	TE
T7870	13856-13862	NN	denotes	buffer
T7871	13863-13864	-LRB-	denotes	(
T7872	13864-13866	CD	denotes	10
T7873	13867-13869	NNP	denotes	mM
T7874	13870-13874	NNP	denotes	Tris
T7875	13875-13878	NNP	denotes	HCl
T7876	13879-13881	NNP	denotes	pH
T7877	13882-13885	CD	denotes	8.0
T7878	13885-13886	,	denotes	,
T7879	13887-13888	CD	denotes	1
T7915	14067-14070	NN	denotes	kit
T7916	14071-14072	-LRB-	denotes	(
T7917	14072-14078	NNP	denotes	QIAGEN
T7918	14078-14079	-RRB-	denotes	)
T7919	14080-14083	CC	denotes	and
T7920	14084-14090	VBD	denotes	eluted
T7921	14091-14093	IN	denotes	in
T7922	14094-14096	CD	denotes	50
T7923	14097-14099	NN	denotes	µl
T7924	14100-14107	JJ	denotes	sterile
T7925	14108-14117	JJ	denotes	distilled
T7926	14118-14123	NN	denotes	water
T7927	14123-14124	.	denotes	.
T7928	14125-14133	JJ	denotes	Specific
T7929	14134-14144	NN	denotes	enrichment
T7930	14145-14147	IN	denotes	in
T7931	14148-14153	JJ	denotes	NF-κB
T7932	14154-14162	NN	denotes	enhancer
T7933	14163-14172	NNS	denotes	sequences
T7934	14173-14176	VBD	denotes	was
T7935	14177-14185	VBN	denotes	measured
T7936	14186-14188	IN	denotes	by
T7937	14189-14198	JJ	denotes	real-time
T7938	14199-14202	NNP	denotes	PCR
T7939	14203-14205	IN	denotes	of
T7940	14206-14215	NN	denotes	chromatin
T7941	14216-14235	NN	denotes	immunoprecipitation
T7942	14236-14237	-LRB-	denotes	(
T7943	14237-14241	NNP	denotes	ChIP
T7944	14241-14242	-RRB-	denotes	)
T7956	14305-14309	VBD	denotes	were
T7957	14310-14317	VBN	denotes	carried
T7958	14318-14321	RP	denotes	out
T7959	14322-14326	IN	denotes	with
T7960	14327-14330	DT	denotes	the
T7961	14331-14338	NNP	denotes	iCycler
T7962	14339-14341	NNP	denotes	iQ
T7963	14342-14351	NNP	denotes	Real-Time
T7964	14352-14361	NN	denotes	detection
T7965	14362-14368	NN	denotes	system
T7966	14369-14370	-LRB-	denotes	(
T7967	14370-14377	NNP	denotes	Bio-Rad
T7968	14378-14390	NNPS	denotes	Laboratories
T7969	14390-14391	-RRB-	denotes	)
T7970	14392-14397	VBG	denotes	using
T7971	14398-14401	DT	denotes	the
T7972	14402-14411	JJ	denotes	following
T7973	14412-14422	NNS	denotes	conditions
T7974	14422-14423	:	denotes	:
T7975	14424-14426	CD	denotes	95
T7976	14426-14427	NN	denotes	°
T7977	14427-14428	NNP	denotes	C
T7978	14428-14429	,	denotes	,
T7979	14430-14431	CD	denotes	1
T7980	14432-14435	NN	denotes	min
T7981	14435-14436	:	denotes	;
T7982	14437-14438	-LRB-	denotes	(
T7983	14438-14440	CD	denotes	94
T7984	14440-14441	NN	denotes	°
T7985	14441-14442	NNP	denotes	C
T7986	14442-14443	,	denotes	,
T7987	14444-14446	CD	denotes	10
T7988	14447-14448	PRP	denotes	s
T7989	14448-14449	:	denotes	;
T7990	14450-14452	CD	denotes	60
T7991	14452-14453	NN	denotes	°
T7992	14453-14454	NNP	denotes	C
T7993	14454-14455	,	denotes	,
T7994	14456-14458	CD	denotes	30
T7995	14459-14460	PRP	denotes	s
T7996	14460-14461	-RRB-	denotes	)
T7997	14462-14463	RB	denotes	×
T7998	14463-14465	CD	denotes	40
T7999	14465-14466	.	denotes	.
T8000	14467-14474	NNS	denotes	Primers
T8001	14475-14479	VBD	denotes	used
T8002	14480-14483	IN	denotes	for
T8003	14484-14490	NNP	denotes	MIP-1α
T8004	14490-14491	,	denotes	,
T8005	14492-14497	NNP	denotes	GAPDH
T8006	14498-14501	CC	denotes	and
T8007	14502-14506	NNP	denotes	ACTB
T8008	14507-14510	VBP	denotes	are
T8009	14511-14517	VBN	denotes	listed
T8010	14518-14520	IN	denotes	in
T8011	14521-14534	NNP	denotes	Supplementary
T8012	14535-14539	NNP	denotes	Data
T8013	14539-14540	.	denotes	.
T8014	14541-14550	JJ	denotes	Real-time
T8015	14551-14554	NNP	denotes	PCR
T8016	14555-14557	IN	denotes	of
T8017	14558-14562	NNP	denotes	CSF3
T8018	14562-14563	,	denotes	,
T8019	14564-14567	NNP	denotes	LTA
T8020	14567-14568	,	denotes	,
T8021	14569-14575	NNP	denotes	NFKBIA
T8022	14576-14579	CC	denotes	and
T8023	14580-14584	CD	denotes	TLR2
T8024	14585-14589	VBD	denotes	were
T8025	14590-14599	VBN	denotes	performed
T8026	14600-14605	VBG	denotes	using
T8027	14606-14609	DT	denotes	the
T8028	14610-14616	NNP	denotes	Custom
T8029	14617-14621	NNP	denotes	ChIP
T8030	14622-14627	NN	denotes	array
T8031	14628-14629	-LRB-	denotes	(
T8032	14629-14635	NNP	denotes	QIAGEN
T8033	14635-14636	-RRB-	denotes	)
T8034	14636-14637	.	denotes	.
T8035	14638-14641	IN	denotes	For
T8036	14642-14646	DT	denotes	each
T8037	14647-14653	NN	denotes	sample
T8038	14653-14654	,	denotes	,
T8039	14655-14661	NNS	denotes	values
T8040	14662-14666	VBD	denotes	were
T8041	14667-14677	VBN	denotes	normalized
T8042	14678-14680	TO	denotes	to
T8043	14681-14686	NN	denotes	input
T8044	14687-14690	NN	denotes	DNA
T8045	14691-14694	CC	denotes	and
T8046	14695-14703	VBD	denotes	reported
T8047	14704-14706	IN	denotes	as
T8048	14707-14708	NN	denotes	%
T8049	14709-14711	IN	denotes	of
T8050	14712-14717	NN	denotes	input
T8051	14718-14722	IN	denotes	over
T8052	14723-14726	DT	denotes	the
T8053	14727-14733	NN	denotes	rabbit
T8054	14734-14737	NNP	denotes	IgG
T8055	14738-14745	NN	denotes	control
T8056	14745-14746	.	denotes	.
T8195	14748-14759	NNP	denotes	Statistical
T8196	14760-14768	NN	denotes	analysis
T8197	14769-14780	NNP	denotes	Statistical
T8198	14781-14789	NN	denotes	analysis
T8199	14790-14793	VBD	denotes	was
T8200	14794-14803	VBN	denotes	performed
T8201	14804-14806	IN	denotes	by
T8202	14807-14815	JJ	denotes	two-tail
T8203	14816-14824	JJ	denotes	unpaired
T8204	14825-14832	NN	denotes	Student
T8205	14832-14834	POS	denotes	's
T8206	14835-14841	JJS	denotes	t-test
T8207	14841-14842	.	denotes	.
T8208	14843-14847	NNS	denotes	Data
T8209	14848-14852	VBD	denotes	were
T8210	14853-14861	VBN	denotes	reported
T8211	14862-14864	IN	denotes	as
T8212	14865-14870	NNS	denotes	means
T8213	14871-14872	VBP	denotes	±
T8214	14873-14875	NNP	denotes	SE
T8215	14875-14876	.	denotes	.
T8216	14877-14888	NNS	denotes	Differences
T8217	14889-14896	IN	denotes	between
T8218	14897-14900	DT	denotes	the
T8219	14901-14906	NNS	denotes	means
T8220	14907-14911	VBD	denotes	were
T8221	14912-14922	VBN	denotes	considered
T8222	14923-14925	IN	denotes	as
T8223	14926-14939	RB	denotes	statistically
T8224	14940-14951	JJ	denotes	significant
T8225	14952-14954	IN	denotes	at
T8226	14955-14958	DT	denotes	the
T8227	14959-14961	CD	denotes	95
T8228	14961-14962	NN	denotes	%
T8229	14963-14968	NN	denotes	level
T8230	14969-14970	-LRB-	denotes	(
T8231	14970-14971	NNP	denotes	P
T8232	14972-14973	CD	denotes	<
T8233	14974-14978	CD	denotes	0.05
T8234	14978-14979	-RRB-	denotes	)
T8235	14979-14980	.	denotes	.
T9532	14991-14994	NNP	denotes	Tat
T9533	14995-15003	VBZ	denotes	enhances
T9534	15004-15007	DT	denotes	the
T9535	15008-15013	JJ	denotes	NF-κB
T9536	15014-15022	NN	denotes	activity
T9537	15023-15025	IN	denotes	by
T9538	15026-15035	VBG	denotes	hijacking
T9539	15036-15041	NNP	denotes	IκB-α
T9540	15042-15045	CC	denotes	and
T9541	15046-15056	VBG	denotes	inhibiting
T9542	15057-15060	DT	denotes	the
T9543	15061-15076	NN	denotes	post-activation
T9544	15077-15081	VB	denotes	turn
T9545	15082-15085	RB	denotes	off
T9546	15086-15088	IN	denotes	of
T9547	15089-15094	NNP	denotes	NF-κB
T9548	15095-15097	PRP	denotes	We
T9549	15098-15106	VBD	denotes	analysed
T9550	15107-15110	DT	denotes	the
T9551	15111-15118	NN	denotes	kinetic
T9552	15119-15121	IN	denotes	of
T9553	15122-15127	JJ	denotes	NF-κB
T9554	15128-15138	NN	denotes	activation
T9555	15139-15141	IN	denotes	in
T9556	15142-15148	JJ	denotes	single
T9557	15149-15154	NN	denotes	round
T9558	15155-15160	NN	denotes	HIV-1
T9559	15161-15170	NN	denotes	infection
T9560	15171-15173	IN	denotes	by
T9561	15174-15184	VBG	denotes	modulating
T9562	15185-15188	DT	denotes	the
T9563	15189-15199	NN	denotes	expression
T9564	15200-15202	IN	denotes	of
T9565	15203-15206	NNP	denotes	Tat
T9566	15207-15211	IN	denotes	with
T9567	15212-15215	NNP	denotes	RNA
T9568	15216-15228	NN	denotes	interference
T9569	15228-15229	.	denotes	.
T9570	15230-15236	NNP	denotes	Jurkat
T9571	15237-15242	NNS	denotes	cells
T9572	15243-15247	VBD	denotes	were
T9573	15248-15259	VBN	denotes	transfected
T9574	15260-15264	IN	denotes	with
T9575	15265-15270	NNP	denotes	siRNA
T9576	15271-15274	NNP	denotes	Tat
T9577	15274-15275	,	denotes	,
T9578	15276-15281	NNP	denotes	siRNA
T9579	15282-15289	NN	denotes	control
T9580	15289-15290	,	denotes	,
T9581	15291-15293	CC	denotes	or
T9582	15294-15298	VBD	denotes	left
T9583	15299-15312	JJ	denotes	untransfected
T9584	15312-15313	,	denotes	,
T9585	15314-15317	CC	denotes	and
T9586	15318-15320	CD	denotes	24
T9587	15321-15322	NN	denotes	h
T9588	15323-15328	RB	denotes	later
T9589	15329-15334	NNS	denotes	cells
T9590	15335-15339	VBD	denotes	were
T9591	15340-15348	VBN	denotes	infected
T9592	15349-15353	IN	denotes	with
T9593	15354-15358	CD	denotes	HXB2
T9594	15359-15374	JJ	denotes	Env-pseudotyped
T9595	15375-15380	NN	denotes	NL4-3
T9596	15380-15381	.	denotes	.
T9597	15381-15386	NNP	denotes	Luc.R
T9598	15386-15387	NNP	denotes	−
T9599	15387-15388	NNP	denotes	E
T9600	15388-15389	VBD	denotes	−
T9601	15390-15397	NNS	denotes	virions
T9602	15397-15398	.	denotes	.
T9603	15399-15401	IN	denotes	By
T9604	15402-15418	JJ	denotes	cytofluorimetric
T9605	15419-15427	NN	denotes	analysis
T9606	15427-15428	,	denotes	,
T9607	15429-15430	RB	denotes	∼
T9608	15430-15432	CD	denotes	40
T9609	15432-15433	NN	denotes	%
T9610	15434-15436	IN	denotes	of
T9611	15437-15442	NNS	denotes	cells
T9612	15443-15446	VBD	denotes	was
T9613	15447-15464	JJ	denotes	siRNA-transfected
T9614	15465-15468	CC	denotes	and
T9615	15469-15483	JJ	denotes	HIV-1-infected
T9616	15484-15486	IN	denotes	at
T9617	15487-15491	JJ	denotes	12-h
T9618	15492-15506	NN	denotes	post-infection
T9619	15507-15508	-LRB-	denotes	(
T9620	15508-15521	NNP	denotes	Supplementary
T9621	15522-15528	NNP	denotes	Figure
T9622	15529-15531	NNP	denotes	S1
T9623	15531-15532	-RRB-	denotes	)
T9624	15532-15533	.	denotes	.
T9625	15534-15537	DT	denotes	The
T9626	15538-15548	NN	denotes	expression
T9627	15549-15551	IN	denotes	of
T9628	15552-15555	NNP	denotes	Tat
T9629	15556-15559	VBD	denotes	was
T9630	15560-15568	VBN	denotes	detected
T9631	15569-15571	IN	denotes	at
T9632	15572-15575	JJ	denotes	1-h
T9633	15576-15590	NN	denotes	post-infection
T9634	15591-15594	CC	denotes	and
T9635	15595-15608	RB	denotes	progressively
T9636	15609-15618	VBD	denotes	increased
T9637	15619-15621	RB	denotes	up
T9638	15622-15624	TO	denotes	to
T9639	15625-15627	CD	denotes	12
T9640	15628-15629	NN	denotes	h
T9641	15630-15632	IN	denotes	in
T9642	15633-15646	JJ	denotes	untransfected
T9643	15647-15650	CC	denotes	and
T9644	15651-15656	JJ	denotes	siRNA
T9645	15657-15676	JJ	denotes	control-transfected
T9646	15677-15682	NNS	denotes	cells
T9647	15682-15683	,	denotes	,
T9648	15684-15689	IN	denotes	while
T9649	15690-15692	PRP	denotes	it
T9650	15693-15696	VBD	denotes	was
T9651	15697-15703	RB	denotes	barely
T9652	15704-15712	VBN	denotes	detected
T9653	15713-15715	IN	denotes	in
T9654	15716-15721	NNP	denotes	siRNA
T9655	15722-15737	VBD	denotes	Tat-transfected
T9656	15738-15743	NNS	denotes	cells
T9657	15744-15745	-LRB-	denotes	(
T9658	15745-15751	NN	denotes	Figure
T9659	15752-15754	CD	denotes	1A
T9660	15754-15755	-RRB-	denotes	)
T9661	15755-15756	.	denotes	.
T9662	15757-15760	DT	denotes	The
T9663	15761-15774	JJ	denotes	LTR-dependent
T9664	15775-15785	NN	denotes	luciferase
T9665	15786-15796	NN	denotes	expression
T9666	15797-15800	VBD	denotes	was
T9667	15801-15805	RB	denotes	also
T9668	15806-15819	RB	denotes	progressively
T9669	15820-15827	VBD	denotes	induced
T9670	15828-15830	IN	denotes	at
T9671	15831-15832	LS	denotes	1
T9672	15832-15833	:	denotes	-
T9673	15834-15836	TO	denotes	to
T9674	15837-15841	JJ	denotes	12-h
T9675	15842-15856	NN	denotes	post-infection
T9676	15857-15859	IN	denotes	in
T9677	15860-15873	JJ	denotes	untransfected
T9678	15874-15877	CC	denotes	and
T9679	15878-15883	JJ	denotes	siRNA
T9680	15884-15903	JJ	denotes	control-transfected
T9681	15904-15909	NNS	denotes	cells
T9682	15909-15910	,	denotes	,
T9683	15911-15916	IN	denotes	while
T9684	15917-15919	PRP	denotes	it
T9685	15920-15923	VBD	denotes	was
T9686	15924-15930	RB	denotes	barely
T9687	15931-15939	VBN	denotes	observed
T9688	15940-15942	IN	denotes	in
T9689	15943-15948	NNP	denotes	siRNA
T9690	15949-15964	VBD	denotes	Tat-transfected
T9691	15965-15970	NNS	denotes	cells
T9692	15970-15971	,	denotes	,
T9693	15972-15974	IN	denotes	as
T9694	15975-15983	VBN	denotes	expected
T9695	15984-15987	IN	denotes	for
T9696	15988-15991	DT	denotes	the
T9697	15992-16005	JJ	denotes	Tat-dependent
T9698	16006-16021	NN	denotes	transactivation
T9699	16022-16024	IN	denotes	of
T9700	16025-16028	DT	denotes	the
T9701	16029-16034	NNP	denotes	HIV-1
T9702	16035-16038	NNP	denotes	LTR
T9703	16039-16040	-LRB-	denotes	(
T9704	16040-16046	NNP	denotes	Figure
T9705	16047-16049	NNP	denotes	1A
T9706	16049-16050	-RRB-	denotes	)
T9707	16050-16051	.	denotes	.
T9708	17245-17248	DT	denotes	The
T9709	17249-17260	JJ	denotes	progressive
T9710	17261-17269	NN	denotes	increase
T9711	17270-17272	IN	denotes	in
T9712	17273-17278	JJ	denotes	NF-κB
T9713	17279-17287	NN	denotes	activity
T9714	17287-17288	,	denotes	,
T9715	17289-17291	IN	denotes	as
T9716	17292-17300	VBN	denotes	measured
T9717	17301-17303	IN	denotes	by
T9718	17304-17307	NNS	denotes	p65
T9719	17308-17311	JJ	denotes	p50
T9720	17312-17319	JJ	denotes	binding
T9721	17320-17322	TO	denotes	to
T9722	17323-17325	DT	denotes	an
T9723	17326-17331	JJ	denotes	NF-κB
T9724	17332-17341	NN	denotes	consensus
T9725	17342-17357	NN	denotes	oligonucleotide
T9726	17358-17359	-LRB-	denotes	(
T9727	17359-17365	NN	denotes	Figure
T9728	17366-17368	NN	denotes	1B
T9729	17368-17369	-RRB-	denotes	)
T9730	17370-17373	CC	denotes	and
T9731	17374-17381	JJ	denotes	nuclear
T9732	17382-17385	NNS	denotes	p65
T9733	17386-17387	-LRB-	denotes	(
T9734	17387-17393	NN	denotes	Figure
T9735	17394-17396	CD	denotes	1C
T9736	17396-17397	,	denotes	,
T9737	17398-17405	NN	denotes	nucleus
T9738	17405-17406	-RRB-	denotes	)
T9739	17406-17407	,	denotes	,
T9740	17408-17411	VBD	denotes	was
T9741	17412-17420	VBN	denotes	observed
T9742	17421-17423	IN	denotes	at
T9743	17424-17425	LS	denotes	1
T9744	17425-17426	:	denotes	-
T9745	17427-17429	TO	denotes	to
T9746	17430-17433	JJ	denotes	3-h
T9747	17434-17448	NN	denotes	post-infection
T9748	17449-17451	IN	denotes	in
T9749	17452-17456	DT	denotes	both
T9750	17457-17469	JJ	denotes	Tat-positive
T9751	17470-17471	-LRB-	denotes	(
T9752	17471-17473	DT	denotes	no
T9753	17474-17479	NN	denotes	siRNA
T9754	17480-17482	CC	denotes	or
T9755	17483-17488	NN	denotes	siRNA
T9756	17489-17496	NN	denotes	control
T9757	17496-17497	-RRB-	denotes	)
T9758	17498-17501	CC	denotes	and
T9759	17502-17514	JJ	denotes	Tat-negative
T9760	17515-17516	-LRB-	denotes	(
T9761	17516-17521	NNP	denotes	siRNA
T9762	17522-17525	NNP	denotes	Tat
T9763	17525-17526	-RRB-	denotes	)
T9764	17527-17532	NNS	denotes	cells
T9765	17532-17533	:	denotes	;
T9766	17534-17541	RB	denotes	however
T9767	17541-17542	,	denotes	,
T9768	17543-17545	IN	denotes	at
T9769	17546-17550	JJ	denotes	12-h
T9770	17551-17565	NN	denotes	post-infection
T9771	17565-17566	,	denotes	,
T9772	17567-17570	DT	denotes	the
T9773	17571-17574	CD	denotes	p65
T9774	17575-17578	NNP	denotes	DNA
T9775	17579-17586	JJ	denotes	binding
T9776	17587-17590	CC	denotes	and
T9777	17591-17598	JJ	denotes	nuclear
T9778	17599-17602	NNS	denotes	p65
T9779	17603-17612	VBD	denotes	persisted
T9780	17613-17621	VBN	denotes	elevated
T9781	17622-17624	IN	denotes	in
T9782	17625-17633	NN	denotes	presence
T9783	17634-17636	IN	denotes	of
T9784	17637-17640	NNP	denotes	Tat
T9785	17641-17642	-LRB-	denotes	(
T9786	17642-17644	DT	denotes	no
T9787	17645-17650	NN	denotes	siRNA
T9788	17651-17654	CC	denotes	and
T9789	17655-17660	NN	denotes	siRNA
T9790	17661-17668	NN	denotes	control
T9791	17668-17669	-RRB-	denotes	)
T9792	17669-17670	,	denotes	,
T9793	17671-17676	IN	denotes	while
T9794	17677-17681	PRP	denotes	they
T9795	17682-17689	VBD	denotes	dropped
T9796	17690-17692	IN	denotes	in
T9797	17693-17700	NN	denotes	absence
T9798	17701-17703	IN	denotes	of
T9799	17704-17707	NNP	denotes	Tat
T9800	17708-17709	-LRB-	denotes	(
T9801	17709-17714	NNP	denotes	siRNA
T9802	17715-17718	NNP	denotes	Tat
T9803	17718-17719	-RRB-	denotes	)
T9804	17720-17721	-LRB-	denotes	(
T9805	17721-17727	NN	denotes	Figure
T9806	17728-17730	CD	denotes	1B
T9807	17731-17734	CC	denotes	and
T9808	17735-17736	NNP	denotes	C
T9809	17736-17737	-RRB-	denotes	)
T9810	17737-17738	.	denotes	.
T9811	17739-17744	NN	denotes	IκB-α
T9812	17745-17748	VBD	denotes	was
T9813	17749-17757	JJ	denotes	degraded
T9814	17758-17760	IN	denotes	in
T9815	17761-17764	DT	denotes	the
T9816	17765-17772	NN	denotes	cytosol
T9817	17773-17779	IN	denotes	within
T9818	17780-17783	JJ	denotes	3-h
T9819	17784-17798	NN	denotes	post-infection
T9820	17799-17802	CC	denotes	and
T9821	17803-17805	IN	denotes	de
T9822	17806-17810	JJ	denotes	novo
T9823	17811-17822	VBN	denotes	synthesized
T9824	17823-17825	IN	denotes	at
T9825	17826-17827	CD	denotes	6
T9826	17828-17829	NN	denotes	h
T9827	17830-17834	IN	denotes	with
T9828	17835-17839	JJ	denotes	full
T9829	17840-17853	NN	denotes	replenishment
T9830	17854-17856	IN	denotes	at
T9831	17857-17861	JJ	denotes	12-h
T9832	17862-17876	NN	denotes	post-infection
T9833	17877-17879	IN	denotes	in
T9834	17880-17884	DT	denotes	both
T9835	17885-17897	JJ	denotes	Tat-positive
T9836	17898-17901	CC	denotes	and
T9837	17902-17914	JJ	denotes	Tat-negative
T9838	17915-17920	NNS	denotes	cells
T9839	17921-17922	-LRB-	denotes	(
T9840	17922-17928	NN	denotes	Figure
T9841	17929-17931	CD	denotes	1C
T9842	17931-17932	,	denotes	,
T9843	17933-17940	NN	denotes	cytosol
T9844	17940-17941	-RRB-	denotes	)
T9845	17941-17942	.	denotes	.
T9846	17943-17955	RB	denotes	Consistently
T9847	17955-17956	,	denotes	,
T9848	17957-17960	DT	denotes	the
T9849	17961-17964	NNP	denotes	IKK
T9850	17965-17973	NN	denotes	activity
T9851	17974-17977	VBD	denotes	was
T9852	17978-17985	VBN	denotes	induced
T9853	17986-17988	IN	denotes	at
T9854	17989-17992	JJ	denotes	1-h
T9855	17993-18007	NN	denotes	post-infection
T9856	18007-18008	,	denotes	,
T9857	18009-18012	CC	denotes	and
T9858	18013-18022	VBD	denotes	decreased
T9859	18023-18025	IN	denotes	at
T9860	18026-18029	JJ	denotes	3-h
T9861	18030-18044	JJ	denotes	post-infection
T9862	18045-18058	RB	denotes	independently
T9863	18059-18061	IN	denotes	of
T9864	18062-18065	DT	denotes	the
T9865	18066-18074	NN	denotes	presence
T9866	18075-18077	IN	denotes	of
T9867	18078-18081	NNP	denotes	Tat
T9868	18082-18083	-LRB-	denotes	(
T9869	18083-18089	NNP	denotes	Figure
T9870	18090-18092	NNP	denotes	1D
T9871	18092-18093	-RRB-	denotes	)
T9872	18093-18094	.	denotes	.
T9873	18095-18102	JJ	denotes	Similar
T9874	18103-18111	NNS	denotes	kinetics
T9875	18112-18114	IN	denotes	of
T9876	18115-18120	JJ	denotes	NF-κB
T9877	18121-18129	NN	denotes	activity
T9878	18129-18130	,	denotes	,
T9879	18131-18134	NNP	denotes	IKK
T9880	18135-18145	NN	denotes	activation
T9881	18146-18149	CC	denotes	and
T9882	18150-18155	JJ	denotes	IκB-α
T9883	18156-18180	NN	denotes	degradation/re-synthesis
T9884	18181-18185	VBD	denotes	were
T9885	18186-18194	VBN	denotes	observed
T9886	18195-18197	IN	denotes	in
T9887	18198-18210	JJ	denotes	single-round
T9888	18211-18216	JJ	denotes	HIV-1
T9889	18217-18226	NN	denotes	infection
T9890	18227-18229	IN	denotes	of
T9891	18230-18235	NNP	denotes	PBMCs
T9892	18236-18241	VBG	denotes	using
T9893	18242-18243	DT	denotes	a
T9894	18244-18252	NN	denotes	cocktail
T9895	18253-18255	IN	denotes	of
T9896	18256-18270	NNP	denotes	Azidothymidine
T9897	18271-18272	-LRB-	denotes	(
T9898	18272-18275	NNP	denotes	AZT
T9899	18275-18276	-RRB-	denotes	)
T9900	18277-18280	CC	denotes	and
T9901	18281-18291	NNP	denotes	Lamivudine
T9902	18292-18293	-LRB-	denotes	(
T9903	18293-18296	NNP	denotes	3TC
T9904	18296-18297	-RRB-	denotes	)
T9905	18298-18300	IN	denotes	as
T9906	18301-18308	NN	denotes	reverse
T9907	18309-18322	NN	denotes	transcriptase
T9908	18323-18333	NNS	denotes	inhibitors
T9909	18334-18335	-LRB-	denotes	(
T9910	18335-18348	NNP	denotes	Supplementary
T9911	18349-18355	NNP	denotes	Figure
T9912	18356-18358	NNP	denotes	S2
T9913	18358-18359	-RRB-	denotes	)
T9914	18359-18360	.	denotes	.
T9915	18361-18366	DT	denotes	These
T9916	18367-18374	NNS	denotes	results
T9917	18375-18384	VBD	denotes	indicated
T9918	18385-18389	IN	denotes	that
T9919	18390-18392	IN	denotes	in
T9920	18393-18405	JJ	denotes	single-round
T9921	18406-18411	JJ	denotes	HIV-1
T9922	18412-18421	NN	denotes	infection
T9923	18422-18425	DT	denotes	the
T9924	18426-18431	NNP	denotes	NF-κB
T9925	18432-18442	NN	denotes	activation
T9926	18443-18452	RB	denotes	initially
T9927	18453-18463	VBD	denotes	correlated
T9928	18464-18468	IN	denotes	with
T9929	18469-18472	DT	denotes	the
T9930	18473-18476	NNP	denotes	IKK
T9931	18477-18487	NN	denotes	activation
T9932	18488-18491	CC	denotes	and
T9933	18492-18497	JJ	denotes	IκB-α
T9934	18498-18509	NN	denotes	degradation
T9935	18510-18523	RB	denotes	independently
T9936	18524-18526	IN	denotes	of
T9937	18527-18530	DT	denotes	the
T9938	18531-18539	NN	denotes	presence
T9939	18540-18542	IN	denotes	of
T9940	18543-18546	NNP	denotes	Tat
T9941	18546-18547	,	denotes	,
T9942	18548-18551	CC	denotes	and
T9943	18552-18554	PRP	denotes	it
T9944	18555-18558	VBD	denotes	was
T9945	18559-18563	VBN	denotes	kept
T9946	18564-18572	VBN	denotes	elevated
T9947	18573-18575	IN	denotes	in
T9948	18576-18584	NN	denotes	presence
T9949	18585-18587	IN	denotes	of
T9950	18588-18591	NNP	denotes	Tat
T9951	18592-18601	VBG	denotes	following
T9952	18602-18605	DT	denotes	the
T9953	18606-18611	NN	denotes	decay
T9954	18612-18614	IN	denotes	of
T9955	18615-18618	DT	denotes	the
T9956	18619-18622	NNP	denotes	IKK
T9957	18623-18631	NN	denotes	activity
T9958	18632-18635	CC	denotes	and
T9959	18636-18639	JJ	denotes	new
T9960	18640-18649	NN	denotes	synthesis
T9961	18650-18652	IN	denotes	of
T9962	18653-18658	NNP	denotes	IκB-α
T9963	18658-18659	.	denotes	.
T9964	18660-18664	JJ	denotes	Next
T9965	18664-18665	,	denotes	,
T9966	18666-18668	PRP	denotes	we
T9967	18669-18675	VBD	denotes	tested
T9968	18676-18679	DT	denotes	the
T9969	18680-18686	JJ	denotes	single
T9970	18687-18693	NN	denotes	action
T9971	18694-18696	IN	denotes	of
T9972	18697-18700	NNP	denotes	Tat
T9973	18701-18703	IN	denotes	on
T9974	18704-18707	DT	denotes	the
T9975	18708-18715	NN	denotes	kinetic
T9976	18716-18718	IN	denotes	of
T9977	18719-18724	JJ	denotes	NF-κB
T9978	18725-18733	NN	denotes	activity
T9979	18734-18741	VBN	denotes	induced
T9980	18742-18744	IN	denotes	by
T9981	18745-18748	NNP	denotes	PMA
T9982	18748-18749	.	denotes	.
T9983	18750-18752	TO	denotes	To
T9984	18753-18757	DT	denotes	this
T9985	18758-18761	NN	denotes	end
T9986	18761-18762	,	denotes	,
T9987	18763-18767	NNP	denotes	HeLa
T9988	18768-18773	NNS	denotes	cells
T9989	18774-18778	VBD	denotes	were
T9990	18779-18790	VBN	denotes	transfected
T9991	18791-18795	IN	denotes	with
T9992	18796-18808	NN	denotes	p3x-FLAG-Tat
T9993	18808-18809	,	denotes	,
T9994	18810-18812	CC	denotes	or
T9995	18813-18818	JJ	denotes	empty
T9996	18819-18825	NN	denotes	vector
T9997	18825-18826	,	denotes	,
T9998	18827-18837	VBN	denotes	stimulated
T9999	18838-18842	IN	denotes	with
T10000	18843-18846	NNP	denotes	PMA
T10001	18847-18850	IN	denotes	for
T10002	18851-18852	CD	denotes	5
T10003	18853-18856	NN	denotes	min
T10004	18856-18857	,	denotes	,
T10005	18858-18861	CC	denotes	and
T10006	18862-18873	RB	denotes	extensively
T10007	18874-18880	VBD	denotes	washed
T10008	18881-18883	TO	denotes	to
T10009	18884-18889	VB	denotes	avoid
T10010	18890-18893	DT	denotes	the
T10011	18894-18905	JJ	denotes	oscillatory
T10012	18906-18913	NN	denotes	kinetic
T10013	18914-18916	IN	denotes	of
T10014	18917-18922	JJ	denotes	NF-κB
T10015	18923-18933	NN	denotes	activation
T10016	18934-18935	-LRB-	denotes	(
T10017	18935-18940	CD	denotes	17,18
T10018	18940-18941	-RRB-	denotes	)
T10019	18941-18942	.	denotes	.
T10020	18943-18945	IN	denotes	In
T10021	18946-18959	JJ	denotes	un-stimulated
T10022	18960-18965	NNS	denotes	cells
T10023	18965-18966	,	denotes	,
T10024	18967-18970	DT	denotes	the
T10025	18971-18974	CD	denotes	p65
T10026	18975-18978	NNP	denotes	DNA
T10027	18979-18986	JJ	denotes	binding
T10028	18987-18988	-LRB-	denotes	(
T10029	18988-18994	NN	denotes	Figure
T10030	18995-18997	NN	denotes	2A
T10031	18997-18998	-RRB-	denotes	)
T10032	18999-19002	CC	denotes	and
T10033	19003-19010	JJ	denotes	nuclear
T10034	19011-19014	NNS	denotes	p65
T10035	19015-19016	-LRB-	denotes	(
T10036	19016-19022	NN	denotes	Figure
T10037	19023-19025	NN	denotes	2B
T10038	19025-19026	,	denotes	,
T10039	19027-19034	NN	denotes	nucleus
T10040	19034-19035	-RRB-	denotes	)
T10041	19036-19040	VBD	denotes	were
T10042	19041-19049	RB	denotes	slightly
T10043	19050-19058	VBN	denotes	enhanced
T10044	19059-19061	IN	denotes	by
T10045	19062-19065	NNP	denotes	Tat
T10046	19065-19066	,	denotes	,
T10047	19067-19072	IN	denotes	while
T10048	19073-19076	DT	denotes	the
T10049	19077-19082	JJ	denotes	IκB-α
T10050	19083-19090	NN	denotes	content
T10051	19091-19094	VBD	denotes	was
T10052	19095-19098	RB	denotes	not
T10053	19099-19112	RB	denotes	significantly
T10054	19113-19121	JJ	denotes	affected
T10055	19122-19123	-LRB-	denotes	(
T10056	19123-19129	NN	denotes	Figure
T10057	19130-19132	NN	denotes	2B
T10058	19132-19133	,	denotes	,
T10059	19134-19141	NN	denotes	cytosol
T10060	19141-19142	-RRB-	denotes	)
T10061	19142-19143	.	denotes	.
T10062	19144-19153	JJ	denotes	Transient
T10063	19154-19165	NN	denotes	stimulation
T10064	19166-19170	IN	denotes	with
T10065	19171-19174	NNP	denotes	PMA
T10066	19175-19184	VBD	denotes	increased
T10067	19185-19188	DT	denotes	the
T10068	19189-19194	NNP	denotes	NF-κB
T10069	19195-19198	NNP	denotes	DNA
T10070	19199-19206	JJ	denotes	binding
T10071	19207-19215	NN	denotes	activity
T10072	19216-19219	CC	denotes	and
T10073	19220-19227	JJ	denotes	nuclear
T10074	19228-19231	NNS	denotes	p65
T10075	19232-19234	IN	denotes	at
T10076	19235-19236	CD	denotes	5
T10077	19237-19240	NN	denotes	min
T10078	19241-19248	VBG	denotes	peaking
T10079	19249-19251	IN	denotes	at
T10080	19252-19254	CD	denotes	60
T10081	19255-19258	CD	denotes	120
T10082	19259-19262	NN	denotes	min
T10083	19263-19276	RB	denotes	independently
T10084	19277-19279	IN	denotes	of
T10085	19280-19283	DT	denotes	the
T10086	19284-19292	NN	denotes	presence
T10087	19293-19295	IN	denotes	of
T10088	19296-19299	NNP	denotes	Tat
T10089	19300-19301	-LRB-	denotes	(
T10090	19301-19307	NNP	denotes	Figure
T10091	19308-19310	NNP	denotes	2A
T10092	19311-19314	CC	denotes	and
T10093	19315-19316	NNP	denotes	B
T10094	19316-19317	-RRB-	denotes	)
T10095	19317-19318	:	denotes	;
T10096	19319-19326	RB	denotes	however
T10097	19326-19327	,	denotes	,
T10098	19328-19333	IN	denotes	while
T10099	19334-19337	DT	denotes	the
T10100	19338-19341	CD	denotes	p65
T10101	19342-19345	NNP	denotes	DNA
T10102	19346-19353	JJ	denotes	binding
T10103	19354-19357	CC	denotes	and
T10104	19358-19365	JJ	denotes	nuclear
T10105	19366-19369	NNS	denotes	p65
T10106	19370-19379	VBD	denotes	persisted
T10107	19380-19388	VBN	denotes	elevated
T10108	19389-19391	IN	denotes	at
T10109	19392-19399	JJ	denotes	240-min
T10110	19400-19414	NN	denotes	post-treatment
T10111	19415-19417	IN	denotes	in
T10112	19418-19430	JJ	denotes	Tat-positive
T10113	19431-19436	NNS	denotes	cells
T10114	19436-19437	,	denotes	,
T10115	19438-19442	PRP	denotes	they
T10116	19443-19450	VBD	denotes	dropped
T10117	19451-19453	IN	denotes	in
T10118	19454-19466	JJ	denotes	Tat-negative
T10119	19467-19472	NNS	denotes	cells
T10120	19473-19474	-LRB-	denotes	(
T10121	19474-19480	NN	denotes	Figure
T10122	19481-19483	CD	denotes	2A
T10123	19484-19487	CC	denotes	and
T10124	19488-19489	NNP	denotes	B
T10125	19489-19490	-RRB-	denotes	)
T10126	19490-19491	.	denotes	.
T10127	19492-19494	IN	denotes	In
T10128	19495-19503	NN	denotes	addition
T10129	19503-19504	,	denotes	,
T10130	19505-19508	NNP	denotes	Tat
T10131	19509-19514	VBD	denotes	bound
T10132	19515-19517	TO	denotes	to
T10133	19518-19521	DT	denotes	the
T10134	19522-19527	JJ	denotes	NF-κB
T10135	19528-19543	NN	denotes	oligonucleotide
T10136	19544-19546	IN	denotes	in
T10137	19547-19560	JJ	denotes	un-stimulated
T10138	19561-19566	NNS	denotes	cells
T10139	19567-19570	CC	denotes	and
T10140	19571-19574	PRP$	denotes	its
T10141	19575-19582	JJ	denotes	binding
T10142	19583-19592	VBN	denotes	increased
T10143	19593-19602	VBG	denotes	following
T10144	19603-19606	NNP	denotes	PMA
T10145	19607-19618	NN	denotes	stimulation
T10146	19619-19620	-LRB-	denotes	(
T10147	19620-19626	NN	denotes	Figure
T10148	19627-19629	NN	denotes	2A
T10149	19629-19630	-RRB-	denotes	)
T10150	19630-19631	,	denotes	,
T10151	19632-19642	VBG	denotes	suggesting
T10152	19643-19647	IN	denotes	that
T10153	19648-19651	DT	denotes	the
T10154	19652-19657	JJ	denotes	viral
T10155	19658-19665	NN	denotes	protein
T10156	19666-19669	VBD	denotes	was
T10157	19670-19671	DT	denotes	a
T10158	19672-19681	NN	denotes	component
T10159	19682-19684	IN	denotes	of
T10160	19685-19688	DT	denotes	the
T10161	19689-19694	JJ	denotes	NF-κB
T10162	19695-19702	JJ	denotes	complex
T10163	19703-19708	VBN	denotes	bound
T10164	19709-19711	TO	denotes	to
T10165	19712-19715	NNP	denotes	DNA
T10166	19715-19716	.	denotes	.
T10167	19717-19728	NN	denotes	Degradation
T10168	19729-19731	IN	denotes	of
T10169	19732-19737	NNP	denotes	IκB-α
T10170	19738-19741	VBD	denotes	was
T10171	19742-19755	RB	denotes	progressively
T10172	19756-19763	VBN	denotes	induced
T10173	19764-19766	IN	denotes	at
T10174	19767-19768	CD	denotes	5
T10175	19769-19771	CD	denotes	30
T10176	19772-19775	NN	denotes	min
T10177	19775-19776	,	denotes	,
T10178	19777-19785	VBN	denotes	followed
T10179	19786-19788	IN	denotes	by
T10180	19789-19791	FW	denotes	de
T10181	19792-19796	FW	denotes	novo
T10182	19797-19806	NN	denotes	synthesis
T10183	19807-19809	IN	denotes	at
T10184	19810-19812	CD	denotes	60
T10185	19813-19816	NN	denotes	min
T10186	19817-19821	IN	denotes	with
T10187	19822-19826	JJ	denotes	full
T10188	19827-19840	NN	denotes	replenishment
T10189	19841-19843	IN	denotes	at
T10190	19844-19847	CD	denotes	240
T10191	19848-19851	NN	denotes	min
T10192	19852-19854	IN	denotes	in
T10193	19855-19859	DT	denotes	both
T10194	19860-19872	JJ	denotes	Tat-positive
T10195	19873-19876	CC	denotes	and
T10196	19877-19885	JJ	denotes	negative
T10197	19886-19891	NNS	denotes	cells
T10198	19892-19893	-LRB-	denotes	(
T10199	19893-19899	NN	denotes	Figure
T10200	19900-19902	NN	denotes	2B
T10201	19902-19903	,	denotes	,
T10202	19904-19911	NN	denotes	cytosol
T10203	19911-19912	-RRB-	denotes	)
T10204	19912-19913	.	denotes	.
T10205	19914-19926	RB	denotes	Consistently
T10206	19926-19927	,	denotes	,
T10207	19928-19931	DT	denotes	the
T10208	19932-19935	NNP	denotes	IKK
T10209	19936-19946	NN	denotes	activation
T10210	19947-19954	VBD	denotes	started
T10211	19955-19957	IN	denotes	at
T10212	19958-19959	CD	denotes	5
T10213	19960-19963	NN	denotes	min
T10214	19963-19964	,	denotes	,
T10215	19965-19968	CC	denotes	and
T10216	19969-19975	VBD	denotes	turned
T10217	19976-19979	RP	denotes	off
T10218	19980-19982	IN	denotes	at
T10219	19983-19985	CD	denotes	30
T10220	19986-19989	NN	denotes	min
T10221	19990-20003	RB	denotes	independently
T10222	20004-20006	IN	denotes	of
T10223	20007-20010	DT	denotes	the
T10224	20011-20019	NN	denotes	presence
T10225	20020-20022	IN	denotes	of
T10226	20023-20026	NNP	denotes	Tat
T10227	20027-20028	-LRB-	denotes	(
T10228	20028-20034	NNP	denotes	Figure
T10229	20035-20037	NNP	denotes	2C
T10230	20037-20038	-RRB-	denotes	)
T10231	20038-20039	.	denotes	.
T10232	20040-20045	DT	denotes	These
T10233	20046-20053	NNS	denotes	results
T10234	20054-20063	VBD	denotes	indicated
T10235	20064-20068	IN	denotes	that
T10236	20069-20072	NNP	denotes	Tat
T10237	20073-20081	VBD	denotes	enhanced
T10238	20082-20085	DT	denotes	the
T10239	20086-20091	JJ	denotes	NF-κB
T10240	20092-20100	NN	denotes	activity
T10241	20101-20103	IN	denotes	in
T10242	20104-20117	JJ	denotes	un-stimulated
T10243	20118-20121	CC	denotes	and
T10244	20122-20136	JJ	denotes	PMA-stimulated
T10245	20137-20142	NNS	denotes	cells
T10246	20143-20150	IN	denotes	without
T10247	20151-20160	VBG	denotes	affecting
T10248	20161-20164	DT	denotes	the
T10249	20165-20168	NNP	denotes	IKK
T10250	20169-20177	NN	denotes	activity
T10251	20178-20181	CC	denotes	and
T10252	20182-20187	JJ	denotes	IκB-α
T10253	20188-20195	NN	denotes	content
T10254	20195-20196	.	denotes	.
T10255	21813-21815	IN	denotes	As
T10256	21816-21818	PRP	denotes	we
T10257	21819-21829	RB	denotes	previously
T10258	21830-21835	VBD	denotes	found
T10259	21836-21840	IN	denotes	that
T10260	21841-21844	NNP	denotes	Tat
T10261	21845-21850	VBZ	denotes	binds
T10262	21851-21853	TO	denotes	to
T10263	21854-21857	DT	denotes	the
T10264	21858-21863	JJ	denotes	sixth
T10265	21864-21871	NN	denotes	ankyrin
T10266	21872-21874	IN	denotes	of
T10267	21875-21880	NNP	denotes	IκB-α
T10268	21881-21882	-LRB-	denotes	(
T10269	21882-21887	CD	denotes	50,51
T10270	21887-21888	-RRB-	denotes	)
T10271	21888-21889	,	denotes	,
T10272	21890-21892	PRP	denotes	we
T10273	21893-21899	VBD	denotes	tested
T10274	21900-21907	IN	denotes	whether
T10275	21908-21911	NNP	denotes	Tat
T10276	21912-21924	VBD	denotes	counteracted
T10277	21925-21928	DT	denotes	the
T10278	21929-21939	NN	denotes	generation
T10279	21940-21942	IN	denotes	of
T10280	21943-21946	DT	denotes	the
T10281	21947-21952	NNP	denotes	IκB-α
T10282	21952-21953	NNP	denotes	/
T10283	21953-21958	NNP	denotes	NF-κB
T10284	21959-21966	NN	denotes	complex
T10285	21966-21967	.	denotes	.
T10286	21968-21970	TO	denotes	To
T10287	21971-21975	DT	denotes	this
T10288	21976-21979	NN	denotes	end
T10289	21979-21980	,	denotes	,
T10290	21981-21984	DT	denotes	the
T10291	21985-21996	NN	denotes	association
T10292	21997-21999	IN	denotes	of
T10293	22000-22005	JJ	denotes	IκB-α
T10294	22006-22010	IN	denotes	with
T10295	22011-22014	CD	denotes	p65
T10296	22015-22018	VBD	denotes	was
T10297	22019-22027	VBN	denotes	analysed
T10298	22028-22030	IN	denotes	in
T10299	22031-22035	NN	denotes	vivo
T10300	22036-22038	IN	denotes	at
T10301	22039-22040	CD	denotes	0
T10302	22041-22044	CC	denotes	and
T10303	22045-22048	CD	denotes	240
T10304	22049-22052	NN	denotes	min
T10305	22053-22058	IN	denotes	after
T10306	22059-22070	JJ	denotes	short-pulse
T10307	22071-22074	NNP	denotes	PMA
T10308	22074-22075	,	denotes	,
T10309	22076-22081	WDT	denotes	which
T10310	22082-22094	VBD	denotes	corresponded
T10311	22095-22097	TO	denotes	to
T10312	22098-22101	DT	denotes	the
T10313	22102-22106	NN	denotes	time
T10314	22107-22109	IN	denotes	of
T10315	22110-22123	JJ	denotes	un-stimulated
T10316	22124-22133	NN	denotes	condition
T10317	22134-22137	CC	denotes	and
T10318	22138-22153	NN	denotes	post-activation
T10319	22154-22156	IN	denotes	de
T10320	22157-22161	NN	denotes	novo
T10321	22162-22171	NN	denotes	synthesis
T10322	22172-22174	IN	denotes	of
T10323	22175-22180	NNP	denotes	IκB-α
T10324	22180-22181	,	denotes	,
T10325	22182-22194	RB	denotes	respectively
T10326	22194-22195	.	denotes	.
T10327	22196-22217	NNP	denotes	Coimmunoprecipitation
T10328	22218-22220	IN	denotes	of
T10329	22221-22226	NNP	denotes	IκB-α
T10330	22227-22231	IN	denotes	with
T10331	22232-22235	CD	denotes	p65
T10332	22236-22239	VBD	denotes	was
T10333	22240-22248	VBN	denotes	detected
T10334	22249-22251	IN	denotes	at
T10335	22252-22253	CD	denotes	0
T10336	22253-22254	:	denotes	-
T10337	22255-22258	CC	denotes	and
T10338	22259-22266	JJ	denotes	240-min
T10339	22267-22281	NN	denotes	post-treatment
T10340	22282-22284	IN	denotes	in
T10341	22285-22297	JJ	denotes	Tat-negative
T10342	22298-22303	NNS	denotes	cells
T10343	22304-22305	-LRB-	denotes	(
T10344	22305-22311	NN	denotes	Figure
T10345	22312-22314	CD	denotes	2D
T10346	22314-22315	,	denotes	,
T10347	22316-22321	NNS	denotes	lanes
T10348	22322-22323	CD	denotes	1
T10349	22324-22327	CC	denotes	and
T10350	22328-22329	CD	denotes	3
T10351	22329-22330	-RRB-	denotes	)
T10352	22330-22331	,	denotes	,
T10353	22332-22335	CC	denotes	and
T10354	22336-22339	VBD	denotes	was
T10355	22340-22346	VBN	denotes	halved
T10356	22347-22349	IN	denotes	in
T10357	22350-22362	JJ	denotes	Tat-positive
T10358	22363-22368	NNS	denotes	cells
T10359	22368-22369	,	denotes	,
T10360	22370-22375	WRB	denotes	where
T10361	22376-22379	NNP	denotes	Tat
T10362	22380-22400	VBD	denotes	coimmunoprecipitated
T10363	22401-22405	IN	denotes	with
T10364	22406-22411	NNP	denotes	IκB-α
T10365	22412-22413	-LRB-	denotes	(
T10366	22413-22419	NNP	denotes	Figure
T10367	22420-22422	NNP	denotes	2D
T10368	22422-22423	,	denotes	,
T10369	22424-22429	NNS	denotes	lanes
T10370	22430-22431	CD	denotes	2
T10371	22432-22435	CC	denotes	and
T10372	22436-22437	CD	denotes	4
T10373	22437-22438	-RRB-	denotes	)
T10374	22438-22439	,	denotes	,
T10375	22440-22450	VBG	denotes	indicating
T10376	22451-22455	IN	denotes	that
T10377	22456-22459	NNP	denotes	Tat
T10378	22460-22468	VBD	denotes	competed
T10379	22469-22472	DT	denotes	the
T10380	22473-22478	JJ	denotes	IκB-α
T10381	22479-22486	JJ	denotes	binding
T10382	22487-22489	TO	denotes	to
T10383	22490-22493	NNS	denotes	p65
T10384	22493-22494	.	denotes	.
T12077	22496-22499	NNP	denotes	Tat
T12078	22500-22511	VBZ	denotes	counteracts
T12079	22512-22515	DT	denotes	the
T12080	22516-22521	JJ	denotes	IκB-α
T12081	22522-22532	NN	denotes	inhibition
T12082	22533-22535	IN	denotes	of
T12083	22536-22539	CD	denotes	p65
T12084	22540-22542	IN	denotes	by
T12085	22543-22552	VBG	denotes	competing
T12086	22553-22556	DT	denotes	the
T12087	22557-22566	NN	denotes	repressor
T12088	22567-22574	JJ	denotes	binding
T12089	22575-22577	PRP	denotes	We
T12090	22578-22585	RB	denotes	further
T12091	22586-22598	VBD	denotes	investigated
T12092	22599-22602	DT	denotes	the
T12093	22603-22611	JJ	denotes	physical
T12094	22612-22623	NN	denotes	interaction
T12095	22624-22626	IN	denotes	of
T12096	22627-22630	NNP	denotes	Tat
T12097	22631-22635	IN	denotes	with
T12098	22636-22641	NNP	denotes	IκB-α
T12099	22642-22647	VBG	denotes	using
T12100	22648-22650	IN	denotes	in
T12101	22651-22656	NN	denotes	vitro
T12102	22657-22667	VBN	denotes	translated
T12103	22668-22676	NNS	denotes	proteins
T12104	22677-22679	IN	denotes	in
T12105	22680-22701	NN	denotes	coimmunoprecipitation
T12106	22702-22708	NNS	denotes	assays
T12107	22708-22709	.	denotes	.
T12108	22710-22713	IN	denotes	For
T12109	22714-22721	VBG	denotes	mapping
T12110	22722-22725	DT	denotes	the
T12111	22726-22737	NN	denotes	interaction
T12112	22738-22745	NNS	denotes	domains
T12113	22745-22746	,	denotes	,
T12114	22747-22749	PRP	denotes	we
T12115	22750-22754	VBD	denotes	used
T12116	22755-22758	DT	denotes	the
T12117	22759-22768	JJ	denotes	following
T12118	22769-22772	NNP	denotes	Tat
T12119	22773-22780	NNS	denotes	mutants
T12120	22780-22781	:	denotes	:
T12121	22782-22785	NNP	denotes	Tat
T12122	22786-22787	NNP	denotes	T
T12123	22787-22788	,	denotes	,
T12124	22788-22789	NNP	denotes	N
T12125	22789-22790	-LRB-	denotes	(
T12126	22790-22795	CD	denotes	23,24
T12127	22795-22796	-RRB-	denotes	)
T12128	22796-22797	DT	denotes	A
T12129	22797-22798	,	denotes	,
T12130	22799-22802	NNP	denotes	Tat
T12131	22803-22804	NNP	denotes	C
T12132	22804-22805	-LRB-	denotes	(
T12133	22805-22813	CD	denotes	22,25,27
T12134	22813-22814	-RRB-	denotes	)
T12135	22814-22815	DT	denotes	A
T12136	22815-22816	,	denotes	,
T12137	22817-22820	NNP	denotes	Tat
T12138	22821-22822	NNP	denotes	K
T12139	22822-22823	-LRB-	denotes	(
T12140	22823-22828	CD	denotes	50,51
T12141	22828-22829	-RRB-	denotes	)
T12142	22829-22830	DT	denotes	A
T12143	22831-22834	CC	denotes	and
T12144	22835-22838	NNP	denotes	Tat
T12145	22839-22840	NNP	denotes	R
T12146	22840-22841	-LRB-	denotes	(
T12147	22841-22843	CD	denotes	49
T12148	22844-22846	CD	denotes	57
T12149	22846-22847	-RRB-	denotes	)
T12150	22847-22848	DT	denotes	A
T12151	22849-22850	-LRB-	denotes	(
T12152	22850-22856	NN	denotes	Figure
T12153	22857-22859	NN	denotes	3A
T12154	22859-22860	-RRB-	denotes	)
T12155	22860-22861	.	denotes	.
T12156	22862-22867	JJ	denotes	IκB-α
T12157	22868-22888	VBN	denotes	coimmunoprecipitated
T12158	22889-22893	IN	denotes	with
T12159	22894-22897	DT	denotes	all
T12160	22898-22901	NNP	denotes	Tat
T12161	22902-22910	NNS	denotes	proteins
T12162	22910-22911	,	denotes	,
T12163	22912-22918	IN	denotes	except
T12164	22919-22922	NNP	denotes	Tat
T12165	22923-22924	NNP	denotes	R
T12166	22924-22925	-LRB-	denotes	(
T12167	22925-22927	CD	denotes	49
T12168	22928-22930	CD	denotes	57
T12169	22930-22931	-RRB-	denotes	)
T12170	22931-22932	DT	denotes	A
T12171	22933-22934	-LRB-	denotes	(
T12172	22934-22940	NN	denotes	Figure
T12173	22941-22943	NN	denotes	3B
T12174	22943-22944	-RRB-	denotes	)
T12175	22944-22945	.	denotes	.
T12176	22946-22951	DT	denotes	These
T12177	22952-22959	NNS	denotes	results
T12178	22960-22969	VBD	denotes	indicated
T12179	22970-22974	IN	denotes	that
T12180	22975-22978	DT	denotes	the
T12181	22979-22992	JJ	denotes	arginine-rich
T12182	22993-22999	NN	denotes	domain
T12183	23000-23002	IN	denotes	of
T12184	23003-23006	NNP	denotes	Tat
T12185	23007-23010	VBD	denotes	was
T12186	23011-23019	VBN	denotes	involved
T12187	23020-23022	IN	denotes	in
T12188	23023-23026	DT	denotes	the
T12189	23027-23034	JJ	denotes	binding
T12190	23035-23037	TO	denotes	to
T12191	23038-23043	NNP	denotes	IκB-α
T12192	23043-23044	,	denotes	,
T12193	23045-23050	WDT	denotes	which
T12194	23051-23054	VBD	denotes	was
T12195	23055-23065	JJ	denotes	consistent
T12196	23066-23070	IN	denotes	with
T12197	23071-23079	JJ	denotes	previous
T12198	23080-23092	NNS	denotes	observations
T12199	23093-23094	-LRB-	denotes	(
T12200	23094-23099	CD	denotes	50,51
T12201	23099-23100	-RRB-	denotes	)
T12202	23100-23101	.	denotes	.
T12203	23102-23110	RB	denotes	Moreover
T12204	23110-23111	,	denotes	,
T12205	23112-23115	NNP	denotes	Tat
T12206	23116-23127	VBG	denotes	associating
T12207	23128-23132	IN	denotes	with
T12208	23133-23138	JJ	denotes	IκB-α
T12209	23139-23147	VBN	denotes	competed
T12210	23148-23151	DT	denotes	the
T12211	23152-23159	JJ	denotes	binding
T12212	23160-23162	IN	denotes	of
T12213	23163-23168	NNP	denotes	IκB-α
T12214	23169-23171	TO	denotes	to
T12215	23172-23175	VB	denotes	p65
T12216	23176-23178	IN	denotes	in
T12217	23179-23180	DT	denotes	a
T12218	23181-23195	JJ	denotes	dose-dependent
T12219	23196-23202	NN	denotes	manner
T12220	23203-23204	-LRB-	denotes	(
T12221	23204-23210	NN	denotes	Figure
T12222	23211-23213	NN	denotes	3C
T12223	23213-23214	,	denotes	,
T12224	23215-23220	NNS	denotes	lanes
T12225	23221-23222	CD	denotes	3
T12226	23223-23224	LS	denotes	5
T12227	23224-23225	-RRB-	denotes	)
T12228	23225-23226	,	denotes	,
T12229	23227-23232	IN	denotes	while
T12230	23233-23236	NNP	denotes	Tat
T12231	23237-23238	NNP	denotes	R
T12232	23238-23239	-LRB-	denotes	(
T12233	23239-23241	CD	denotes	49
T12234	23242-23244	CD	denotes	57
T12235	23244-23245	-RRB-	denotes	)
T12236	23245-23246	DT	denotes	A
T12237	23246-23247	,	denotes	,
T12238	23248-23253	WDT	denotes	which
T12239	23254-23260	VBD	denotes	lacked
T12240	23261-23264	DT	denotes	the
T12241	23265-23272	JJ	denotes	binding
T12242	23273-23277	NN	denotes	site
T12243	23278-23281	IN	denotes	for
T12244	23282-23287	NNP	denotes	IκB-α
T12245	23287-23288	,	denotes	,
T12246	23289-23292	VBD	denotes	did
T12247	23293-23296	RB	denotes	not
T12248	23297-23306	JJ	denotes	associate
T12249	23307-23311	IN	denotes	with
T12250	23312-23317	NNP	denotes	IκB-α
T12251	23318-23321	CC	denotes	and
T12252	23322-23325	VBD	denotes	did
T12253	23326-23329	RB	denotes	not
T12254	23330-23337	VB	denotes	compete
T12255	23338-23341	DT	denotes	the
T12256	23342-23349	JJ	denotes	binding
T12257	23350-23352	IN	denotes	of
T12258	23353-23358	NNP	denotes	IκB-α
T12259	23359-23361	TO	denotes	to
T12260	23362-23365	VB	denotes	p65
T12261	23366-23367	-LRB-	denotes	(
T12262	23367-23373	NN	denotes	Figure
T12263	23374-23376	NN	denotes	3C
T12264	23376-23377	,	denotes	,
T12265	23378-23383	NNS	denotes	lanes
T12266	23384-23385	CD	denotes	6
T12267	23386-23387	CD	denotes	8
T12268	23387-23388	-RRB-	denotes	)
T12269	23388-23389	.	denotes	.
T12270	25484-25488	JJ	denotes	Next
T12271	25488-25489	,	denotes	,
T12272	25490-25492	PRP	denotes	we
T12273	25493-25502	VBD	denotes	evaluated
T12274	25503-25510	IN	denotes	whether
T12275	25511-25514	NNP	denotes	Tat
T12276	25515-25527	VBD	denotes	counteracted
T12277	25528-25531	DT	denotes	the
T12278	25532-25546	NNP	denotes	IκB-α-mediated
T12279	25547-25557	NN	denotes	inhibition
T12280	25558-25560	IN	denotes	of
T12281	25561-25564	CD	denotes	p65
T12282	25565-25572	JJ	denotes	binding
T12283	25573-25575	TO	denotes	to
T12284	25576-25579	NNP	denotes	DNA
T12285	25580-25582	IN	denotes	by
T12286	25583-25593	VBG	denotes	incubating
T12287	25594-25596	IN	denotes	in
T12288	25597-25602	NN	denotes	vitro
T12289	25603-25613	VBN	denotes	translated
T12290	25614-25617	NNS	denotes	p65
T12291	25618-25621	CC	denotes	and
T12292	25622-25627	JJ	denotes	IκB-α
T12293	25628-25636	NNS	denotes	proteins
T12294	25637-25641	IN	denotes	with
T12295	25642-25645	DT	denotes	the
T12296	25646-25651	NNP	denotes	NF-κB
T12297	25652-25657	NN	denotes	probe
T12298	25658-25660	IN	denotes	in
T12299	25661-25669	NN	denotes	presence
T12300	25670-25672	CC	denotes	or
T12301	25673-25680	NN	denotes	absence
T12302	25681-25683	IN	denotes	of
T12303	25684-25687	NNP	denotes	Tat
T12304	25688-25696	VBD	denotes	followed
T12305	25697-25699	IN	denotes	by
T12306	25700-25704	NNP	denotes	EMSA
T12307	25704-25705	.	denotes	.
T12308	25706-25709	DT	denotes	The
T12309	25710-25713	CD	denotes	p65
T12310	25714-25717	NNP	denotes	DNA
T12311	25718-25725	JJ	denotes	binding
T12312	25726-25734	NN	denotes	activity
T12313	25735-25738	VBD	denotes	was
T12314	25739-25748	VBN	denotes	inhibited
T12315	25749-25751	IN	denotes	by
T12316	25752-25757	NNP	denotes	IκB-α
T12317	25758-25761	CC	denotes	and
T12318	25762-25770	VBN	denotes	restored
T12319	25771-25773	IN	denotes	in
T12320	25774-25775	DT	denotes	a
T12321	25776-25790	JJ	denotes	dose-dependent
T12322	25791-25797	NN	denotes	manner
T12323	25798-25800	IN	denotes	by
T12324	25801-25810	JJ	denotes	wild-type
T12325	25811-25814	NNP	denotes	Tat
T12326	25814-25815	,	denotes	,
T12327	25816-25819	CC	denotes	and
T12328	25820-25823	RB	denotes	not
T12329	25824-25826	IN	denotes	by
T12330	25827-25830	NNP	denotes	Tat
T12331	25831-25832	NNP	denotes	R
T12332	25832-25833	-LRB-	denotes	(
T12333	25833-25835	CD	denotes	49
T12334	25836-25838	CD	denotes	57
T12335	25838-25839	-RRB-	denotes	)
T12336	25840-25841	-LRB-	denotes	(
T12337	25841-25847	NNP	denotes	Figure
T12338	25848-25850	NNP	denotes	3D
T12339	25850-25851	-RRB-	denotes	)
T12340	25851-25852	.	denotes	.
T12341	25853-25860	RB	denotes	Further
T12342	25860-25861	,	denotes	,
T12343	25862-25864	PRP	denotes	we
T12344	25865-25873	VBD	denotes	analysed
T12345	25874-25877	DT	denotes	the
T12346	25878-25881	NNP	denotes	Tat
T12347	25882-25888	NN	denotes	effect
T12348	25889-25891	IN	denotes	on
T12349	25892-25895	DT	denotes	the
T12350	25896-25901	JJ	denotes	IκB-α
T12351	25902-25912	NN	denotes	repression
T12352	25913-25915	IN	denotes	of
T12353	25916-25919	CD	denotes	p65
T12354	25920-25935	JJ	denotes	transcriptional
T12355	25936-25944	NN	denotes	activity
T12356	25945-25947	IN	denotes	by
T12357	25948-25960	VBG	denotes	transfecting
T12358	25961-25964	CD	denotes	p50
T12359	25964-25965	FW	denotes	−
T12360	25965-25966	FW	denotes	/
T12361	25966-25967	FW	denotes	−
T12362	25967-25970	FW	denotes	p65
T12363	25970-25971	FW	denotes	−
T12364	25971-25972	FW	denotes	/
T12365	25972-25973	FW	denotes	−
T12366	25973-25977	FW	denotes	MEFs
T12367	25978-25982	IN	denotes	with
T12368	25983-25986	DT	denotes	the
T12369	25987-25996	JJ	denotes	NF-κB-Luc
T12370	25997-26005	NN	denotes	reporter
T12371	26006-26014	RB	denotes	together
T12372	26015-26019	IN	denotes	with
T12373	26020-26030	NN	denotes	expression
T12374	26031-26038	NNS	denotes	vectors
T12375	26039-26041	IN	denotes	of
T12376	26042-26045	CD	denotes	p65
T12377	26046-26049	CC	denotes	and
T12378	26050-26055	NNP	denotes	IκB-α
T12379	26055-26056	,	denotes	,
T12380	26057-26059	IN	denotes	in
T12381	26060-26068	NN	denotes	presence
T12382	26069-26071	CC	denotes	or
T12383	26072-26079	NN	denotes	absence
T12384	26080-26082	IN	denotes	of
T12385	26083-26086	NNP	denotes	Tat
T12386	26086-26087	.	denotes	.
T12387	26088-26093	JJ	denotes	IκB-α
T12388	26094-26103	VBD	denotes	inhibited
T12389	26104-26107	DT	denotes	the
T12390	26108-26121	JJ	denotes	p65-dependent
T12391	26122-26132	NN	denotes	expression
T12392	26133-26135	IN	denotes	of
T12393	26136-26139	DT	denotes	the
T12394	26140-26150	NN	denotes	luciferase
T12395	26151-26155	NN	denotes	gene
T12396	26155-26156	,	denotes	,
T12397	26157-26162	WDT	denotes	which
T12398	26163-26166	VBD	denotes	was
T12399	26167-26175	VBN	denotes	restored
T12400	26176-26178	IN	denotes	in
T12401	26179-26180	DT	denotes	a
T12402	26181-26195	JJ	denotes	dose-dependent
T12403	26196-26202	NN	denotes	manner
T12404	26203-26205	IN	denotes	by
T12405	26206-26215	JJ	denotes	wild-type
T12406	26216-26219	NNP	denotes	Tat
T12407	26219-26220	,	denotes	,
T12408	26221-26224	CC	denotes	and
T12409	26225-26228	RB	denotes	not
T12410	26229-26231	IN	denotes	by
T12411	26232-26235	NNP	denotes	Tat
T12412	26236-26237	NNP	denotes	R
T12413	26237-26238	-LRB-	denotes	(
T12414	26238-26240	CD	denotes	49
T12415	26241-26243	CD	denotes	57
T12416	26243-26244	-RRB-	denotes	)
T12417	26244-26245	DT	denotes	A
T12418	26246-26247	-LRB-	denotes	(
T12419	26247-26253	NN	denotes	Figure
T12420	26254-26256	CD	denotes	3E
T12421	26256-26257	-RRB-	denotes	)
T12422	26257-26258	.	denotes	.
T12423	26259-26269	RB	denotes	Altogether
T12424	26270-26275	DT	denotes	these
T12425	26276-26283	NNS	denotes	results
T12426	26284-26293	VBD	denotes	indicated
T12427	26294-26298	IN	denotes	that
T12428	26299-26302	NNP	denotes	Tat
T12429	26303-26314	VBZ	denotes	counteracts
T12430	26315-26318	DT	denotes	the
T12431	26319-26324	JJ	denotes	IκB-α
T12432	26325-26335	NN	denotes	repression
T12433	26336-26338	IN	denotes	of
T12434	26339-26342	DT	denotes	the
T12435	26343-26346	CD	denotes	p65
T12436	26347-26358	JJ	denotes	DNA-binding
T12437	26359-26362	CC	denotes	and
T12438	26363-26378	JJ	denotes	transcriptional
T12439	26379-26387	NN	denotes	activity
T12440	26388-26390	IN	denotes	by
T12441	26391-26402	VBG	denotes	associating
T12442	26403-26407	IN	denotes	with
T12443	26408-26413	NNP	denotes	IκB-α
T12444	26414-26417	CC	denotes	and
T12445	26418-26427	VBG	denotes	competing
T12446	26428-26431	DT	denotes	the
T12447	26432-26441	NN	denotes	repressor
T12448	26442-26449	JJ	denotes	binding
T12449	26450-26452	TO	denotes	to
T12450	26453-26456	NNS	denotes	p65
T12451	26456-26457	.	denotes	.
T13857	26459-26462	NNP	denotes	Tat
T13858	26463-26472	VBZ	denotes	increases
T13859	26473-26476	DT	denotes	the
T13860	26477-26480	CD	denotes	p65
T13861	26481-26489	NN	denotes	affinity
T13862	26490-26497	JJ	denotes	binding
T13863	26498-26500	TO	denotes	to
T13864	26501-26504	NNP	denotes	DNA
T13865	26505-26512	IN	denotes	through
T13866	26513-26524	NN	denotes	association
T13867	26525-26529	IN	denotes	with
T13868	26530-26533	CD	denotes	p65
T13869	26534-26536	PRP	denotes	We
T13870	26537-26543	VBD	denotes	tested
T13871	26544-26551	IN	denotes	whether
T13872	26552-26555	NNP	denotes	Tat
T13873	26556-26566	RB	denotes	physically
T13874	26567-26577	VBD	denotes	interacted
T13875	26578-26582	IN	denotes	with
T13876	26583-26586	NNS	denotes	p65
T13877	26586-26587	.	denotes	.
T13878	26588-26592	NNP	denotes	HeLa
T13879	26593-26598	NNS	denotes	cells
T13880	26599-26603	VBD	denotes	were
T13881	26604-26615	VBN	denotes	transfected
T13882	26616-26620	IN	denotes	with
T13883	26621-26624	DT	denotes	the
T13884	26625-26635	NN	denotes	expression
T13885	26636-26643	NNS	denotes	vectors
T13886	26644-26646	IN	denotes	of
T13887	26647-26653	NNP	denotes	HA-p65
T13888	26654-26657	CC	denotes	and
T13889	26658-26666	NNP	denotes	FLAG-Tat
T13890	26666-26667	,	denotes	,
T13891	26668-26671	CC	denotes	and
T13892	26672-26674	CD	denotes	24
T13893	26675-26676	NN	denotes	h
T13894	26677-26682	RB	denotes	later
T13895	26683-26686	NNP	denotes	Tat
T13896	26687-26690	VBD	denotes	was
T13897	26691-26709	VBN	denotes	immunoprecipitated
T13898	26710-26714	IN	denotes	from
T13899	26715-26719	NN	denotes	cell
T13900	26720-26728	NNS	denotes	extracts
T13901	26728-26729	.	denotes	.
T13902	26730-26733	DT	denotes	The
T13903	26734-26737	CD	denotes	p65
T13904	26738-26745	NN	denotes	protein
T13905	26746-26749	VBD	denotes	was
T13906	26750-26770	VBN	denotes	coimmunoprecipitated
T13907	26771-26775	IN	denotes	with
T13908	26776-26785	JJ	denotes	wild-type
T13909	26786-26789	NNP	denotes	Tat
T13910	26790-26792	CC	denotes	or
T13911	26793-26796	DT	denotes	the
T13912	26797-26804	NNS	denotes	mutants
T13913	26805-26808	NNP	denotes	Tat
T13914	26809-26810	NNP	denotes	T
T13915	26810-26811	,	denotes	,
T13916	26811-26812	NNP	denotes	N
T13917	26812-26813	-LRB-	denotes	(
T13918	26813-26818	CD	denotes	23,24
T13919	26818-26819	-RRB-	denotes	)
T13920	26819-26820	DT	denotes	A
T13921	26820-26821	,	denotes	,
T13922	26822-26825	NNP	denotes	Tat
T13923	26826-26827	NNP	denotes	K
T13924	26827-26828	-LRB-	denotes	(
T13925	26828-26833	CD	denotes	50,51
T13926	26833-26834	-RRB-	denotes	)
T13927	26834-26835	DT	denotes	A
T13928	26836-26839	CC	denotes	and
T13929	26840-26843	NNP	denotes	Tat
T13930	26844-26845	NNP	denotes	R
T13942	26845-26846	-LRB-	denotes	(
T13943	26846-26848	CD	denotes	49
T13944	26849-26851	CD	denotes	57
T13945	26851-26852	-RRB-	denotes	)
T13946	26852-26853	DT	denotes	A
T13947	26854-26856	IN	denotes	at
T13948	26857-26864	JJ	denotes	similar
T13949	26865-26871	NNS	denotes	levels
T13950	26871-26872	,	denotes	,
T13951	26873-26878	IN	denotes	while
T13952	26879-26880	DT	denotes	a
T13953	26881-26892	JJ	denotes	significant
T13954	26893-26901	NN	denotes	decrease
T13955	26902-26904	IN	denotes	in
T13956	26905-26926	NN	denotes	coimmunoprecipitation
T13957	26927-26930	VBD	denotes	was
T13958	26931-26939	VBN	denotes	observed
T13959	26940-26943	IN	denotes	for
T13960	26944-26947	NNP	denotes	Tat
T13961	26948-26949	NNP	denotes	C
T13962	26949-26950	-LRB-	denotes	(
T13963	26950-26958	CD	denotes	22,25,27
T13964	26958-26959	-RRB-	denotes	)
T13965	26959-26960	DT	denotes	A
T13966	26961-26962	-LRB-	denotes	(
T13967	26962-26968	NN	denotes	Figure
T13968	26969-26971	NN	denotes	4A
T13969	26971-26972	-RRB-	denotes	)
T13970	26972-26973	.	denotes	.
T13971	26974-26986	RB	denotes	Consistently
T13972	26986-26987	,	denotes	,
T13973	26988-26996	NNP	denotes	GST-pull
T13974	26997-27001	IN	denotes	down
T13975	27002-27004	IN	denotes	of
T13976	27005-27007	IN	denotes	in
T13977	27008-27013	NN	denotes	vitro
T13978	27014-27024	VBN	denotes	translated
T13979	27025-27033	NNS	denotes	proteins
T13980	27034-27040	VBD	denotes	showed
T13981	27041-27044	DT	denotes	the
T13982	27045-27051	JJ	denotes	direct
T13983	27052-27059	JJ	denotes	binding
T13984	27060-27062	IN	denotes	of
T13985	27063-27066	CD	denotes	p65
T13986	27067-27069	TO	denotes	to
T13987	27070-27079	JJ	denotes	wild-type
T13988	27080-27083	NNP	denotes	Tat
T13989	27083-27084	,	denotes	,
T13990	27085-27087	CC	denotes	or
T13991	27088-27091	DT	denotes	the
T13992	27092-27099	NNS	denotes	mutants
T13993	27100-27103	NNP	denotes	Tat
T13994	27104-27105	NNP	denotes	T
T13995	27105-27106	,	denotes	,
T13996	27106-27107	NNP	denotes	N
T13997	27107-27108	-LRB-	denotes	(
T13998	27108-27113	CD	denotes	23,24
T13999	27113-27114	-RRB-	denotes	)
T14000	27114-27115	DT	denotes	A
T14001	27115-27116	,	denotes	,
T14002	27117-27120	NNP	denotes	Tat
T14003	27121-27122	NNP	denotes	K
T14004	27122-27123	-LRB-	denotes	(
T14005	27123-27128	CD	denotes	50,51
T14006	27128-27129	-RRB-	denotes	)
T14007	27129-27130	DT	denotes	A
T14008	27131-27134	CC	denotes	and
T14009	27135-27138	NNP	denotes	Tat
T14010	27139-27140	NNP	denotes	R
T14011	27140-27141	-LRB-	denotes	(
T14012	27141-27143	CD	denotes	49
T14013	27144-27146	CD	denotes	57
T14014	27146-27147	-RRB-	denotes	)
T14015	27147-27148	DT	denotes	A
T14016	27148-27149	,	denotes	,
T14017	27150-27153	CC	denotes	and
T14018	27154-27158	NN	denotes	lack
T14019	27159-27161	IN	denotes	of
T14020	27162-27169	JJ	denotes	binding
T14021	27170-27172	TO	denotes	to
T14022	27173-27176	NNP	denotes	Tat
T14023	27177-27178	NNP	denotes	C
T14024	27178-27179	-LRB-	denotes	(
T14025	27179-27187	CD	denotes	22,25,27
T14026	27187-27188	-RRB-	denotes	)
T14027	27188-27189	DT	denotes	A
T14028	27190-27191	-LRB-	denotes	(
T14029	27191-27197	NN	denotes	Figure
T14030	27198-27200	NN	denotes	4B
T14031	27200-27201	-RRB-	denotes	)
T14032	27201-27202	.	denotes	.
T14033	27203-27208	DT	denotes	These
T14034	27209-27216	NNS	denotes	results
T14035	27217-27226	VBD	denotes	indicated
T14036	27227-27231	IN	denotes	that
T14037	27232-27235	NNP	denotes	Tat
T14038	27236-27246	VBN	denotes	associated
T14039	27247-27251	IN	denotes	with
T14040	27252-27255	CD	denotes	p65
T14041	27256-27263	IN	denotes	through
T14042	27264-27267	DT	denotes	the
T14043	27268-27281	JJ	denotes	cysteine-rich
T14044	27282-27288	NN	denotes	domain
T14045	27289-27292	CC	denotes	and
T14046	27293-27302	NN	denotes	ruled-out
T14047	27303-27306	DT	denotes	the
T14048	27307-27318	NN	denotes	requirement
T14049	27319-27321	IN	denotes	of
T14050	27322-27330	VBG	denotes	bridging
T14051	27331-27339	NNS	denotes	proteins
T14052	27340-27342	TO	denotes	to
T14053	27343-27350	VB	denotes	mediate
T14054	27351-27355	JJ	denotes	such
T14055	27356-27367	NN	denotes	interaction
T14056	27367-27368	.	denotes	.
T14057	29093-29095	TO	denotes	To
T14058	29096-29104	VB	denotes	identify
T14059	29105-29108	DT	denotes	the
T14060	29109-29112	NNS	denotes	p65
T14061	29113-29119	NN	denotes	domain
T14062	29120-29128	VBN	denotes	required
T14063	29129-29132	IN	denotes	for
T14064	29133-29140	JJ	denotes	binding
T14065	29141-29143	TO	denotes	to
T14066	29144-29147	NNP	denotes	Tat
T14067	29147-29148	,	denotes	,
T14068	29149-29153	NNP	denotes	HeLa
T14069	29154-29159	NNS	denotes	cells
T14070	29160-29164	VBD	denotes	were
T14071	29165-29176	VBN	denotes	transfected
T14072	29177-29181	IN	denotes	with
T14073	29182-29190	JJ	denotes	FLAG-Tat
T14074	29191-29199	RB	denotes	together
T14075	29200-29204	IN	denotes	with
T14076	29205-29211	JJ	denotes	HA-p65
T14077	29212-29219	NNS	denotes	mutants
T14078	29219-29220	,	denotes	,
T14079	29221-29226	WDT	denotes	which
T14080	29227-29231	VBD	denotes	were
T14081	29232-29239	VBN	denotes	deleted
T14082	29240-29242	IN	denotes	of
T14083	29243-29246	DT	denotes	the
T14084	29247-29262	NN	denotes	transactivation
T14085	29263-29269	NN	denotes	domain
T14086	29270-29271	NNP	denotes	[
T14087	29271-29276	NNP	denotes	p65ΔC
T14088	29277-29278	-LRB-	denotes	(
T14089	29278-29279	CD	denotes	1
T14090	29280-29283	CD	denotes	318
T14091	29283-29284	-RRB-	denotes	)
T14092	29284-29285	NNP	denotes	]
T14093	29285-29286	,	denotes	,
T14094	29287-29289	CC	denotes	or
T14095	29290-29293	DT	denotes	the
T14096	29294-29297	NNP	denotes	RHD
T14097	29298-29299	NNP	denotes	[
T14098	29299-29304	NNP	denotes	p65ΔN
T14099	29305-29306	-LRB-	denotes	(
T14100	29306-29309	CD	denotes	122
T14101	29310-29313	CD	denotes	551
T14102	29313-29314	-RRB-	denotes	)
T14103	29314-29315	NNP	denotes	]
T14104	29316-29317	-LRB-	denotes	(
T14105	29317-29323	NNP	denotes	Figure
T14106	29324-29326	NNP	denotes	4C
T14107	29326-29327	-RRB-	denotes	)
T14108	29327-29328	.	denotes	.
T14109	29329-29332	NNP	denotes	Tat
T14110	29333-29353	VBD	denotes	coimmunoprecipitated
T14111	29354-29358	IN	denotes	with
T14112	29359-29364	NNP	denotes	p65ΔC
T14113	29365-29366	-LRB-	denotes	(
T14114	29366-29367	CD	denotes	1
T14115	29368-29371	CD	denotes	318
T14116	29371-29372	-RRB-	denotes	)
T14117	29372-29373	,	denotes	,
T14118	29374-29377	CC	denotes	and
T14119	29378-29381	RB	denotes	not
T14120	29382-29386	IN	denotes	with
T14121	29387-29392	NNP	denotes	p65ΔN
T14122	29393-29394	-LRB-	denotes	(
T14123	29394-29397	CD	denotes	122
T14124	29398-29401	CD	denotes	551
T14125	29401-29402	-RRB-	denotes	)
T14126	29403-29404	-LRB-	denotes	(
T14127	29404-29410	NN	denotes	Figure
T14128	29411-29413	CD	denotes	4D
T14129	29413-29414	-RRB-	denotes	)
T14130	29414-29415	,	denotes	,
T14131	29416-29426	VBG	denotes	indicating
T14132	29427-29431	IN	denotes	that
T14133	29432-29435	DT	denotes	the
T14134	29436-29439	NNP	denotes	RHD
T14135	29440-29442	IN	denotes	of
T14136	29443-29446	CD	denotes	p65
T14137	29447-29450	VBD	denotes	was
T14138	29451-29459	VBN	denotes	involved
T14139	29460-29462	IN	denotes	in
T14140	29463-29466	DT	denotes	the
T14141	29467-29475	JJ	denotes	physical
T14142	29476-29487	NN	denotes	interaction
T14143	29488-29492	IN	denotes	with
T14144	29493-29496	NNP	denotes	Tat
T14145	29496-29497	.	denotes	.
T14146	29498-29500	TO	denotes	To
T14147	29501-29508	VB	denotes	address
T14148	29509-29512	DT	denotes	the
T14149	29513-29519	NN	denotes	effect
T14150	29520-29522	IN	denotes	of
T14151	29523-29526	NNP	denotes	Tat
T14152	29527-29529	IN	denotes	on
T14153	29530-29533	DT	denotes	the
T14154	29534-29537	CD	denotes	p65
T14155	29538-29549	JJ	denotes	DNA-binding
T14156	29550-29558	NN	denotes	affinity
T14157	29558-29559	,	denotes	,
T14158	29560-29568	VBN	denotes	purified
T14159	29569-29580	JJ	denotes	recombinant
T14160	29581-29584	NNS	denotes	p65
T14161	29585-29592	NN	denotes	protein
T14162	29593-29596	VBD	denotes	was
T14163	29597-29606	VBN	denotes	incubated
T14164	29607-29611	IN	denotes	with
T14165	29612-29629	JJ	denotes	32P-labeled-NF-κB
T14166	29630-29645	JJ	denotes	double-stranded
T14167	29646-29661	NN	denotes	oligonucleotide
T14168	29662-29664	IN	denotes	in
T14169	29665-29673	NN	denotes	presence
T14170	29674-29676	CC	denotes	or
T14171	29677-29684	NN	denotes	absence
T14172	29685-29687	IN	denotes	of
T14173	29688-29697	JJ	denotes	wild-type
T14174	29698-29701	NNP	denotes	Tat
T14175	29702-29704	CC	denotes	or
T14176	29705-29708	NNP	denotes	Tat
T14177	29709-29710	NNP	denotes	C
T14178	29710-29711	-LRB-	denotes	(
T14179	29711-29719	CD	denotes	22,25,27
T14180	29719-29720	-RRB-	denotes	)
T14181	29720-29721	DT	denotes	A
T14182	29721-29722	,	denotes	,
T14183	29723-29726	CC	denotes	and
T14184	29727-29730	CD	denotes	p65
T14185	29731-29742	JJ	denotes	DNA-binding
T14186	29743-29746	VBD	denotes	was
T14187	29747-29755	VBN	denotes	analysed
T14188	29756-29758	IN	denotes	by
T14189	29759-29763	NNP	denotes	EMSA
T14190	29763-29764	.	denotes	.
T14191	29765-29772	IN	denotes	Whereas
T14192	29773-29782	JJ	denotes	wild-type
T14193	29783-29786	NNP	denotes	Tat
T14194	29787-29800	RB	denotes	significantly
T14195	29801-29810	VBD	denotes	increased
T14196	29811-29814	DT	denotes	the
T14197	29815-29822	JJ	denotes	binding
T14198	29823-29825	IN	denotes	of
T14199	29826-29829	CD	denotes	p65
T14200	29830-29832	TO	denotes	to
T14201	29833-29836	NNP	denotes	DNA
T14202	29836-29837	,	denotes	,
T14203	29838-29841	DT	denotes	the
T14204	29842-29848	JJ	denotes	mutant
T14205	29849-29852	NNP	denotes	Tat
T14206	29853-29854	NNP	denotes	C
T14207	29854-29855	-LRB-	denotes	(
T14208	29855-29863	CD	denotes	22,25,27
T14209	29863-29864	-RRB-	denotes	)
T14210	29864-29865	DT	denotes	A
T14211	29865-29866	,	denotes	,
T14212	29867-29874	VBG	denotes	lacking
T14213	29875-29878	DT	denotes	the
T14214	29879-29886	JJ	denotes	binding
T14215	29887-29891	NN	denotes	site
T14216	29892-29895	IN	denotes	for
T14217	29896-29899	NNS	denotes	p65
T14218	29899-29900	,	denotes	,
T14219	29901-29904	VBD	denotes	was
T14220	29905-29916	JJ	denotes	ineffective
T14221	29917-29918	-LRB-	denotes	(
T14222	29918-29924	NN	denotes	Figure
T14223	29925-29927	CD	denotes	4E
T14224	29927-29928	-RRB-	denotes	)
T14225	29928-29929	.	denotes	.
T14226	29930-29933	DT	denotes	The
T14227	29934-29937	CD	denotes	p65
T14228	29938-29945	JJ	denotes	binding
T14229	29946-29948	TO	denotes	to
T14230	29949-29952	NNP	denotes	DNA
T14231	29953-29956	VBD	denotes	was
T14232	29957-29966	VBN	denotes	evaluated
T14233	29967-29969	IN	denotes	by
T14234	29970-29981	NN	denotes	competition
T14235	29982-29986	IN	denotes	with
T14236	29987-29997	VBG	denotes	increasing
T14237	29998-30005	NNS	denotes	amounts
T14238	30006-30008	IN	denotes	of
T14239	30009-30013	JJ	denotes	cold
T14240	30014-30024	NN	denotes	competitor
T14241	30025-30030	NN	denotes	NF-κB
T14242	30031-30046	NN	denotes	oligonucleotide
T14243	30046-30047	,	denotes	,
T14244	30048-30053	WDT	denotes	which
T14245	30054-30060	VBD	denotes	showed
T14246	30061-30065	IN	denotes	that
T14247	30066-30069	NNP	denotes	Tat
T14248	30070-30083	RB	denotes	significantly
T14249	30084-30092	VBD	denotes	enhanced
T14250	30093-30096	DT	denotes	the
T14251	30097-30100	CD	denotes	p65
T14252	30101-30109	NN	denotes	affinity
T14253	30110-30117	JJ	denotes	binding
T14254	30118-30120	TO	denotes	to
T14255	30121-30124	NNP	denotes	DNA
T14256	30125-30126	-LRB-	denotes	(
T14257	30126-30132	NNP	denotes	Figure
T14258	30133-30135	NNP	denotes	4E
T14259	30136-30139	CC	denotes	and
T14260	30140-30141	NN	denotes	F
T14261	30141-30142	-RRB-	denotes	)
T14262	30142-30143	.	denotes	.
T15998	30145-30148	NNP	denotes	Tat
T15999	30149-30158	VBZ	denotes	activates
T16000	30159-30162	DT	denotes	the
T16001	30163-30176	JJ	denotes	p65-dependent
T16002	30177-30187	NN	denotes	expression
T16003	30188-30190	IN	denotes	of
T16004	30191-30207	JJ	denotes	NF-κB-responsive
T16005	30208-30213	NNS	denotes	genes
T16006	30213-30214	,	denotes	,
T16007	30215-30223	VBZ	denotes	occupies
T16008	30224-30227	DT	denotes	the
T16009	30228-30233	JJ	denotes	NF-κB
T16010	30234-30243	NNS	denotes	enhancers
T16011	30244-30247	CC	denotes	and
T16012	30248-30256	VBZ	denotes	promotes
T16013	30257-30260	DT	denotes	the
T16014	30261-30264	CD	denotes	p65
T16015	30265-30276	NN	denotes	recruitment
T16016	30277-30281	IN	denotes	with
T16017	30282-30287	JJ	denotes	IκB-α
T16018	30288-30300	NN	denotes	displacement
T16019	30301-30303	PRP	denotes	We
T16020	30304-30312	VBD	denotes	analysed
T16021	30313-30316	DT	denotes	the
T16022	30317-30323	NN	denotes	action
T16023	30324-30326	IN	denotes	of
T16024	30327-30330	NNP	denotes	Tat
T16025	30331-30333	IN	denotes	on
T16026	30334-30337	DT	denotes	the
T16027	30338-30348	NN	denotes	expression
T16028	30349-30351	IN	denotes	of
T16029	30352-30368	JJ	denotes	NF-κB-responsive
T16030	30369-30374	NNS	denotes	genes
T16031	30375-30377	IN	denotes	in
T16032	30378-30382	NN	denotes	vivo
T16033	30382-30383	.	denotes	.
T16034	30384-30386	TO	denotes	To
T16035	30387-30391	DT	denotes	this
T16036	30392-30395	NN	denotes	end
T16037	30395-30396	,	denotes	,
T16038	30397-30401	NNP	denotes	HeLa
T16039	30402-30407	NNS	denotes	cells
T16040	30408-30412	VBD	denotes	were
T16041	30413-30424	VBN	denotes	transfected
T16042	30425-30429	IN	denotes	with
T16043	30430-30438	NNP	denotes	FLAG-Tat
T16044	30438-30439	,	denotes	,
T16045	30440-30448	NNP	denotes	FLAG-Tat
T16046	30449-30450	NNP	denotes	C
T16047	30450-30451	-LRB-	denotes	(
T16048	30451-30459	CD	denotes	22,25,27
T16049	30459-30460	-RRB-	denotes	)
T16050	30460-30461	DT	denotes	A
T16051	30462-30464	CC	denotes	or
T16052	30465-30473	JJ	denotes	FLAG-Tat
T16053	30474-30475	NN	denotes	R
T16054	30475-30476	-LRB-	denotes	(
T16055	30476-30478	CD	denotes	49
T16056	30479-30481	CD	denotes	57
T16057	30481-30482	-RRB-	denotes	)
T16058	30482-30483	DT	denotes	A
T16059	30483-30484	,	denotes	,
T16060	30485-30488	CC	denotes	and
T16061	30489-30491	CD	denotes	48
T16062	30492-30493	NN	denotes	h
T16063	30494-30499	RB	denotes	later
T16064	30500-30503	DT	denotes	the
T16065	30504-30514	NN	denotes	expression
T16066	30515-30517	IN	denotes	of
T16067	30518-30519	DT	denotes	a
T16068	30520-30526	NN	denotes	number
T16069	30527-30529	IN	denotes	of
T16070	30530-30545	JJ	denotes	NF-κB-dependent
T16071	30546-30551	NNS	denotes	genes
T16072	30552-30555	VBD	denotes	was
T16073	30556-30564	VBN	denotes	analysed
T16074	30565-30567	IN	denotes	by
T16075	30568-30577	JJ	denotes	real-time
T16076	30578-30581	NNP	denotes	PCR
T16077	30581-30582	.	denotes	.
T16078	30583-30586	NNP	denotes	Tat
T16079	30587-30600	RB	denotes	significantly
T16080	30601-30610	VBD	denotes	increased
T16081	30611-30614	DT	denotes	the
T16082	30615-30625	NN	denotes	expression
T16083	30626-30628	IN	denotes	of
T16084	30629-30635	NNP	denotes	MIP-1α
T16085	30635-30636	,	denotes	,
T16086	30637-30641	NNP	denotes	CSF3
T16087	30641-30642	,	denotes	,
T16088	30643-30646	NNP	denotes	LTA
T16089	30646-30647	,	denotes	,
T16090	30648-30654	NNP	denotes	NFKBIA
T16091	30655-30658	CC	denotes	and
T16092	30659-30663	NNP	denotes	TLR2
T16093	30663-30664	,	denotes	,
T16094	30665-30670	IN	denotes	while
T16095	30671-30674	DT	denotes	the
T16096	30675-30682	NNS	denotes	mutants
T16097	30683-30686	NNP	denotes	Tat
T16098	30687-30688	NNP	denotes	C
T16099	30688-30689	-LRB-	denotes	(
T16100	30689-30697	CD	denotes	22,25,27
T16101	30697-30698	-RRB-	denotes	)
T16102	30698-30699	DT	denotes	A
T16103	30700-30703	CC	denotes	and
T16104	30704-30707	NNP	denotes	Tat
T16105	30708-30709	NNP	denotes	R
T16106	30709-30710	-LRB-	denotes	(
T16107	30710-30712	CD	denotes	49
T16108	30713-30715	CD	denotes	57
T16109	30715-30716	-RRB-	denotes	)
T16110	30716-30717	DT	denotes	A
T16111	30718-30722	VBD	denotes	were
T16112	30723-30734	JJ	denotes	ineffective
T16113	30735-30736	-LRB-	denotes	(
T16114	30736-30742	NN	denotes	Figure
T16115	30743-30745	CD	denotes	5A
T16116	30746-30747	NNP	denotes	E
T16117	30747-30748	-RRB-	denotes	)
T16118	30748-30749	.	denotes	.
T16119	30750-30753	DT	denotes	The
T16120	30754-30767	JJ	denotes	Tat-dependent
T16121	30768-30783	NN	denotes	transactivation
T16122	30784-30786	IN	denotes	of
T16123	30787-30790	DT	denotes	the
T16124	30791-30806	JJ	denotes	NF-κB-dependent
T16125	30807-30812	NNS	denotes	genes
T16126	30813-30816	VBD	denotes	was
T16127	30817-30826	VBN	denotes	abolished
T16128	30827-30831	WRB	denotes	when
T16129	30832-30837	NNS	denotes	cells
T16130	30838-30842	VBD	denotes	were
T16131	30843-30854	VBN	denotes	transfected
T16132	30855-30859	IN	denotes	with
T16133	30860-30865	NNP	denotes	siRNA
T16134	30866-30869	CD	denotes	p65
T16135	30869-30870	,	denotes	,
T16136	30871-30881	VBG	denotes	indicating
T16137	30882-30886	IN	denotes	that
T16138	30887-30890	DT	denotes	the
T16139	30891-30894	NNP	denotes	Tat
T16140	30895-30910	JJ	denotes	transcriptional
T16141	30911-30921	NN	denotes	activation
T16142	30922-30925	VBD	denotes	was
T16143	30926-30934	VBN	denotes	mediated
T16144	30935-30937	IN	denotes	by
T16145	30938-30941	NNS	denotes	p65
T16146	30942-30943	-LRB-	denotes	(
T16147	30943-30949	NN	denotes	Figure
T16148	30950-30952	CD	denotes	5A
T16149	30953-30954	NNP	denotes	E
T16150	30954-30955	-RRB-	denotes	)
T16151	30955-30956	.	denotes	.
T16152	30957-30968	RB	denotes	Differently
T16153	30968-30969	,	denotes	,
T16154	30970-30973	DT	denotes	the
T16155	30974-30984	NN	denotes	expression
T16156	30985-30987	IN	denotes	of
T16157	30988-30991	DT	denotes	the
T16158	30992-31009	JJ	denotes	NF-κB-independent
T16159	31010-31015	NNS	denotes	genes
T16160	31016-31021	NNP	denotes	GAPDH
T16161	31022-31025	CC	denotes	and
T16162	31026-31030	NNP	denotes	ACTB
T16163	31031-31034	VBD	denotes	was
T16164	31035-31045	JJ	denotes	unaffected
T16165	31046-31048	IN	denotes	by
T16166	31049-31058	JJ	denotes	wild-type
T16167	31059-31062	CC	denotes	and
T16168	31063-31066	NNP	denotes	Tat
T16169	31067-31074	NNS	denotes	mutants
T16170	31075-31076	-LRB-	denotes	(
T16171	31076-31082	NN	denotes	Figure
T16172	31083-31085	CD	denotes	5F
T16173	31086-31089	CC	denotes	and
T16174	31090-31091	NNP	denotes	G
T16175	31091-31092	-RRB-	denotes	)
T16176	31092-31093	.	denotes	.
T16177	31094-31099	DT	denotes	These
T16178	31100-31107	NNS	denotes	results
T16179	31108-31112	VBD	denotes	were
T16180	31113-31123	JJ	denotes	consistent
T16181	31124-31128	IN	denotes	with
T16182	31129-31132	DT	denotes	the
T16183	31133-31144	NN	denotes	requirement
T16184	31145-31147	IN	denotes	of
T16185	31148-31152	DT	denotes	both
T16186	31153-31156	DT	denotes	the
T16187	31157-31170	NN	denotes	cysteine-rich
T16188	31171-31174	CC	denotes	and
T16189	31175-31188	JJ	denotes	arginine-rich
T16190	31189-31196	NNS	denotes	domains
T16191	31197-31199	IN	denotes	of
T16192	31200-31203	NNP	denotes	Tat
T16193	31204-31206	TO	denotes	to
T16194	31207-31214	VB	denotes	enhance
T16195	31215-31218	DT	denotes	the
T16196	31219-31224	NN	denotes	NF-κB
T16197	31225-31233	NN	denotes	activity
T16198	31233-31234	,	denotes	,
T16199	31235-31245	VBG	denotes	suggesting
T16200	31246-31250	IN	denotes	that
T16201	31251-31254	DT	denotes	the
T16202	31255-31268	NN	denotes	up-regulation
T16203	31269-31271	IN	denotes	of
T16204	31272-31288	JJ	denotes	NF-κB-responsive
T16205	31289-31294	NNS	denotes	genes
T16206	31295-31297	IN	denotes	by
T16207	31298-31301	NNP	denotes	Tat
T16208	31302-31307	MD	denotes	might
T16209	31308-31313	VB	denotes	occur
T16210	31314-31321	IN	denotes	through
T16211	31322-31330	JJ	denotes	physical
T16212	31331-31342	NN	denotes	interaction
T16213	31343-31345	IN	denotes	of
T16214	31346-31349	DT	denotes	the
T16215	31350-31355	JJ	denotes	viral
T16216	31356-31363	NN	denotes	protein
T16217	31364-31368	IN	denotes	with
T16218	31369-31372	CD	denotes	p65
T16219	31373-31376	CC	denotes	and
T16220	31377-31382	NNP	denotes	IκB-α
T16221	31382-31383	.	denotes	.
T16222	32978-32984	NN	denotes	MIP-1α
T16223	32984-32985	,	denotes	,
T16224	32986-32991	WDT	denotes	which
T16225	32992-32995	VBD	denotes	was
T16226	32996-32999	DT	denotes	the
T16227	33000-33006	RB	denotes	mostly
T16228	33007-33016	VBN	denotes	activated
T16229	33017-33021	NN	denotes	gene
T16230	33022-33024	IN	denotes	by
T16231	33025-33028	NNP	denotes	Tat
T16232	33028-33029	,	denotes	,
T16233	33030-33037	VBZ	denotes	encodes
T16234	33038-33041	IN	denotes	for
T16235	33042-33043	DT	denotes	a
T16236	33044-33053	NN	denotes	chemokine
T16237	33054-33058	WDT	denotes	that
T16238	33059-33067	VBZ	denotes	promotes
T16239	33068-33071	DT	denotes	the
T16240	33072-33083	NN	denotes	recruitment
T16241	33084-33086	IN	denotes	of
T16242	33087-33103	JJ	denotes	pro-inflammatory
T16243	33104-33109	NNS	denotes	cells
T16244	33110-33111	-LRB-	denotes	(
T16245	33111-33113	CD	denotes	56
T16246	33113-33114	-RRB-	denotes	)
T16247	33114-33115	.	denotes	.
T16248	33116-33128	RB	denotes	Consistently
T16249	33129-33133	IN	denotes	with
T16250	33134-33137	PRP$	denotes	our
T16251	33138-33146	NNS	denotes	findings
T16252	33146-33147	,	denotes	,
T16253	33148-33154	JJ	denotes	MIP-1α
T16254	33155-33165	NN	denotes	expression
T16255	33166-33169	VBD	denotes	was
T16256	33170-33179	VBN	denotes	increased
T16257	33180-33182	IN	denotes	in
T16258	33183-33188	JJ	denotes	glial
T16259	33189-33194	NNS	denotes	cells
T16260	33195-33198	CC	denotes	and
T16261	33199-33204	JJ	denotes	lymph
T16262	33205-33210	NNS	denotes	nodes
T16263	33211-33213	IN	denotes	of
T16264	33214-33218	NNP	denotes	AIDS
T16265	33219-33227	NNS	denotes	patients
T16266	33228-33229	-LRB-	denotes	(
T16267	33229-33234	CD	denotes	57,58
T16268	33234-33235	-RRB-	denotes	)
T16269	33235-33236	:	denotes	;
T16270	33237-33245	RB	denotes	moreover
T16271	33245-33246	,	denotes	,
T16272	33247-33253	JJ	denotes	MIP-1α
T16273	33254-33264	NN	denotes	production
T16274	33265-33268	VBD	denotes	was
T16275	33269-33276	VBN	denotes	induced
T16276	33277-33279	IN	denotes	by
T16277	33280-33285	NNP	denotes	HIV-1
T16278	33286-33295	NN	denotes	infection
T16279	33296-33297	-LRB-	denotes	(
T16280	33297-33302	CD	denotes	59,60
T16281	33302-33303	-RRB-	denotes	)
T16282	33304-33307	CC	denotes	and
T16283	33308-33311	NNP	denotes	Tat
T16284	33312-33319	NN	denotes	protein
T16285	33320-33321	-LRB-	denotes	(
T16286	33321-33329	CD	denotes	57,61,62
T16287	33329-33330	-RRB-	denotes	)
T16288	33330-33331	.	denotes	.
T16289	33332-33335	DT	denotes	The
T16290	33336-33351	JJ	denotes	transcriptional
T16291	33352-33362	NN	denotes	regulation
T16292	33363-33365	IN	denotes	of
T16293	33366-33372	NN	denotes	MIP-1α
T16294	33373-33376	VBZ	denotes	has
T16295	33377-33381	VBN	denotes	been
T16296	33382-33388	RB	denotes	poorly
T16297	33389-33402	VBN	denotes	characterized
T16298	33402-33403	.	denotes	.
T16299	33404-33416	JJ	denotes	Jaspar-based
T16300	33417-33425	NN	denotes	analysis
T16301	33426-33427	-LRB-	denotes	(
T16302	33427-33453	NN	denotes	http://jaspar.genereg.net/
T16303	33453-33454	-RRB-	denotes	)
T16304	33455-33464	VBD	denotes	predicted
T16305	33465-33470	CD	denotes	three
T16306	33471-33479	JJ	denotes	putative
T16307	33480-33485	NN	denotes	NF-κB
T16308	33486-33495	NNS	denotes	enhancers
T16309	33496-33498	IN	denotes	in
T16310	33499-33502	DT	denotes	the
T16311	33503-33511	JJ	denotes	proximal
T16312	33512-33520	NN	denotes	promoter
T16313	33521-33527	NN	denotes	region
T16314	33528-33530	IN	denotes	of
T16315	33531-33534	DT	denotes	the
T16316	33535-33541	JJ	denotes	MIP-1α
T16317	33542-33546	NN	denotes	gene
T16318	33546-33547	:	denotes	:
T16319	33548-33554	NN	denotes	NF-κB1
T16320	33554-33555	,	denotes	,
T16321	33556-33557	VBP	denotes	−
T16322	33557-33561	CD	denotes	1023
T16323	33561-33562	CD	denotes	/
T16324	33562-33563	NN	denotes	−
T16325	33563-33567	CD	denotes	1014
T16326	33568-33579	NNS	denotes	nucleotides
T16327	33579-33580	:	denotes	;
T16328	33581-33587	NNP	denotes	NF-κB2
T16329	33587-33588	,	denotes	,
T16330	33589-33590	CD	denotes	−
T16331	33590-33593	CD	denotes	661
T16332	33593-33594	NN	denotes	/
T16333	33594-33595	NN	denotes	−
T16334	33595-33598	CD	denotes	652
T16335	33599-33610	NNS	denotes	nucleotides
T16336	33610-33611	:	denotes	;
T16337	33612-33618	NNP	denotes	NF-κB3
T16338	33618-33619	,	denotes	,
T16339	33620-33624	CD	denotes	+370
T16340	33624-33625	NN	denotes	/
T16341	33625-33629	CD	denotes	+379
T16342	33630-33641	NNS	denotes	nucleotides
T16343	33642-33643	-LRB-	denotes	(
T16344	33643-33656	NNP	denotes	Supplementary
T16345	33657-33663	NNP	denotes	Figure
T16346	33664-33667	NNP	denotes	S3A
T16347	33667-33668	-RRB-	denotes	)
T16348	33668-33669	.	denotes	.
T16349	33670-33672	TO	denotes	To
T16350	33673-33681	VB	denotes	validate
T16351	33682-33685	DT	denotes	the
T16352	33686-33695	VBN	denotes	predicted
T16353	33696-33701	NN	denotes	NF-κB
T16354	33702-33709	JJ	denotes	binding
T16355	33710-33715	NNS	denotes	sites
T16356	33716-33718	IN	denotes	of
T16357	33719-33722	DT	denotes	the
T16358	33723-33729	JJ	denotes	MIP-1α
T16359	33730-33738	NN	denotes	promoter
T16360	33738-33739	,	denotes	,
T16361	33740-33742	PRP	denotes	we
T16362	33743-33753	VBD	denotes	stimulated
T16363	33754-33758	NNP	denotes	HeLa
T16364	33759-33764	NNS	denotes	cells
T16365	33765-33769	IN	denotes	with
T16366	33770-33775	NNP	denotes	TNF-α
T16367	33775-33776	,	denotes	,
T16368	33777-33778	DT	denotes	a
T16369	33779-33789	JJ	denotes	well-known
T16370	33790-33795	NN	denotes	NF-κB
T16371	33796-33803	NN	denotes	inducer
T16372	33803-33804	,	denotes	,
T16373	33805-33808	CC	denotes	and
T16374	33809-33817	VBD	denotes	measured
T16375	33818-33821	DT	denotes	the
T16376	33822-33832	NN	denotes	expression
T16377	33833-33839	NNS	denotes	levels
T16378	33840-33842	IN	denotes	of
T16379	33843-33849	NN	denotes	MIP-1α
T16380	33850-33852	IN	denotes	by
T16381	33853-33862	JJ	denotes	real-time
T16382	33863-33866	NNP	denotes	PCR
T16383	33866-33867	,	denotes	,
T16384	33868-33871	CC	denotes	and
T16385	33872-33875	DT	denotes	the
T16386	33876-33879	NNS	denotes	p65
T16387	33880-33889	NN	denotes	occupancy
T16388	33890-33892	IN	denotes	of
T16389	33893-33896	DT	denotes	the
T16390	33897-33902	NNP	denotes	NF-κB
T16391	33903-33908	NNS	denotes	sites
T16392	33909-33911	IN	denotes	by
T16393	33912-33916	NNP	denotes	ChIP
T16394	33916-33917	.	denotes	.
T16395	33918-33923	JJ	denotes	TNF-α
T16396	33924-33933	VBN	denotes	activated
T16397	33934-33937	DT	denotes	the
T16398	33938-33948	NN	denotes	expression
T16399	33949-33951	IN	denotes	of
T16400	33952-33958	NN	denotes	MIP-1α
T16401	33959-33962	CC	denotes	and
T16402	33963-33970	VBD	denotes	induced
T16403	33971-33974	DT	denotes	the
T16404	33975-33978	CD	denotes	p65
T16405	33979-33990	NN	denotes	recruitment
T16406	33991-33993	TO	denotes	to
T16407	33994-33997	DT	denotes	the
T16408	33998-34004	JJ	denotes	NF-κB1
T16409	34005-34009	NN	denotes	site
T16410	34010-34012	IN	denotes	of
T16411	34013-34019	NN	denotes	MIP-1α
T16412	34020-34027	IN	denotes	without
T16413	34028-34037	VBG	denotes	affecting
T16414	34038-34041	DT	denotes	the
T16415	34042-34051	NN	denotes	occupancy
T16416	34052-34054	IN	denotes	of
T16417	34055-34058	DT	denotes	the
T16418	34059-34067	JJ	denotes	putative
T16419	34068-34074	NN	denotes	NF-κB2
T16420	34075-34078	CC	denotes	and
T16421	34079-34085	JJ	denotes	NF-κB3
T16422	34086-34091	NNS	denotes	sites
T16423	34092-34093	-LRB-	denotes	(
T16424	34093-34106	NNP	denotes	Supplementary
T16425	34107-34113	NNP	denotes	Figure
T16426	34114-34117	NNP	denotes	S3B
T16427	34118-34121	CC	denotes	and
T16428	34122-34125	NNP	denotes	S3C
T16429	34125-34126	-RRB-	denotes	)
T16430	34126-34127	.	denotes	.
T16431	34128-34131	NNP	denotes	Tat
T16432	34132-34136	RB	denotes	also
T16433	34137-34145	VBD	denotes	promoted
T16434	34146-34149	DT	denotes	the
T16435	34150-34161	NN	denotes	recruitment
T16436	34162-34164	IN	denotes	of
T16437	34165-34168	CD	denotes	p65
T16438	34169-34171	TO	denotes	to
T16439	34172-34175	DT	denotes	the
T16440	34176-34182	JJ	denotes	NF-κB1
T16441	34183-34187	NN	denotes	site
T16442	34188-34190	IN	denotes	of
T16443	34191-34197	NNP	denotes	MIP-1α
T16444	34197-34198	,	denotes	,
T16445	34199-34204	IN	denotes	while
T16446	34205-34207	PRP	denotes	it
T16447	34208-34211	VBD	denotes	did
T16448	34212-34215	RB	denotes	not
T16449	34216-34222	VB	denotes	affect
T16450	34223-34226	DT	denotes	the
T16451	34227-34235	JJ	denotes	putative
T16452	34236-34242	NN	denotes	NF-κB2
T16453	34243-34246	CC	denotes	and
T16454	34247-34253	JJ	denotes	NF-κB3
T16455	34254-34259	NNS	denotes	sites
T16456	34260-34261	-LRB-	denotes	(
T16457	34261-34274	NNP	denotes	Supplementary
T16458	34275-34281	NNP	denotes	Figure
T16459	34282-34285	NNP	denotes	S3D
T16460	34285-34286	-RRB-	denotes	)
T16461	34286-34287	.	denotes	.
T16462	34288-34298	RB	denotes	Altogether
T16463	34299-34304	DT	denotes	these
T16464	34305-34312	NNS	denotes	results
T16465	34313-34322	VBD	denotes	indicated
T16466	34323-34327	IN	denotes	that
T16467	34328-34331	DT	denotes	the
T16468	34332-34338	JJ	denotes	NF-κB1
T16469	34339-34343	NN	denotes	site
T16470	34344-34347	VBD	denotes	was
T16471	34348-34351	DT	denotes	the
T16472	34352-34356	JJ	denotes	only
T16473	34357-34366	JJ	denotes	effective
T16474	34367-34372	NN	denotes	NF-κB
T16475	34373-34381	NN	denotes	enhancer
T16476	34382-34384	IN	denotes	of
T16477	34385-34388	DT	denotes	the
T16478	34389-34395	JJ	denotes	MIP-1α
T16479	34396-34404	NN	denotes	promoter
T16480	34405-34407	IN	denotes	in
T16481	34408-34416	NN	denotes	response
T16482	34417-34419	TO	denotes	to
T16483	34420-34425	NNP	denotes	TNF-α
T16484	34426-34429	CC	denotes	and
T16485	34430-34433	NNP	denotes	Tat
T16486	34433-34434	.	denotes	.
T16487	34435-34444	RB	denotes	Similarly
T16488	34445-34447	TO	denotes	to
T16489	34448-34454	NNP	denotes	MIP-1α
T16490	34454-34455	,	denotes	,
T16491	34456-34459	NNP	denotes	Tat
T16492	34460-34469	VBD	denotes	increased
T16493	34470-34473	DT	denotes	the
T16494	34474-34485	NN	denotes	recruitment
T16495	34486-34488	IN	denotes	of
T16496	34489-34492	CD	denotes	p65
T16497	34493-34495	TO	denotes	to
T16498	34496-34499	DT	denotes	the
T16499	34500-34505	JJ	denotes	NF-κB
T16500	34506-34515	NNS	denotes	enhancers
T16501	34516-34518	IN	denotes	of
T16502	34519-34523	NNP	denotes	CSF3
T16503	34523-34524	,	denotes	,
T16504	34525-34528	NNP	denotes	LTA
T16505	34528-34529	,	denotes	,
T16506	34530-34536	NNP	denotes	NFKBIA
T16507	34537-34540	CC	denotes	and
T16508	34541-34545	NNP	denotes	TLR2
T16509	34545-34546	,	denotes	,
T16510	34547-34552	IN	denotes	while
T16511	34553-34555	PRP	denotes	it
T16512	34556-34559	VBD	denotes	was
T16513	34560-34571	JJ	denotes	ineffective
T16514	34572-34574	IN	denotes	at
T16515	34575-34578	DT	denotes	the
T16516	34579-34588	NNS	denotes	promoters
T16517	34589-34591	IN	denotes	of
T16518	34592-34597	NNP	denotes	GAPDH
T16519	34598-34601	CC	denotes	and
T16520	34602-34606	NNP	denotes	ACTB
T16521	34607-34608	-LRB-	denotes	(
T16522	34608-34614	NNP	denotes	Figure
T16523	34615-34617	NNP	denotes	5H
T16524	34617-34618	-RRB-	denotes	)
T16525	34618-34619	.	denotes	.
T16526	34620-34622	IN	denotes	By
T16527	34623-34627	NNP	denotes	ChIP
T16528	34627-34628	,	denotes	,
T16529	34629-34632	NNP	denotes	Tat
T16530	34633-34638	VBD	denotes	bound
T16531	34639-34641	TO	denotes	to
T16532	34642-34645	DT	denotes	the
T16533	34646-34662	JJ	denotes	NF-κB-responsive
T16534	34663-34672	NNS	denotes	promoters
T16535	34672-34673	,	denotes	,
T16536	34674-34677	CC	denotes	and
T16537	34678-34681	RB	denotes	not
T16538	34682-34684	TO	denotes	to
T16539	34685-34688	DT	denotes	the
T16540	34689-34694	NNP	denotes	GAPDH
T16541	35067-35076	VBD	denotes	displaced
T16542	34695-34698	CC	denotes	and
T16543	34699-34703	NNP	denotes	ACTB
T16544	34704-34713	NNS	denotes	promoters
T16545	34713-34714	,	denotes	,
T16546	34715-34719	IN	denotes	with
T16547	34720-34724	NN	denotes	loss
T16548	34725-34727	IN	denotes	of
T16549	34728-34735	JJ	denotes	binding
T16550	34736-34745	VBG	denotes	following
T16551	34746-34749	CD	denotes	p65
T16552	34750-34753	NNP	denotes	RNA
T16553	34754-34766	NN	denotes	interference
T16554	34767-34768	-LRB-	denotes	(
T16555	34768-34774	NNP	denotes	Figure
T16556	34775-34777	NNP	denotes	5I
T16557	34777-34778	-RRB-	denotes	)
T16558	34778-34779	,	denotes	,
T16559	34780-34790	VBG	denotes	suggesting
T16560	34791-34795	IN	denotes	that
T16561	34796-34799	NNP	denotes	Tat
T16562	34800-34809	NN	denotes	occupancy
T16563	34810-34818	VBD	denotes	occurred
T16564	34819-34822	IN	denotes	via
T16565	34823-34826	CD	denotes	p65
T16566	34827-34838	NN	denotes	interaction
T16567	34838-34839	.	denotes	.
T16568	34840-34842	IN	denotes	As
T16569	34843-34853	JJ	denotes	additional
T16570	34854-34862	NNS	denotes	findings
T16571	34862-34863	,	denotes	,
T16572	34864-34869	NNP	denotes	IκB-α
T16573	34870-34873	VBD	denotes	was
T16574	34874-34902	VBN	denotes	chromatin-immunoprecipitated
T16575	34903-34905	IN	denotes	at
T16576	34906-34909	DT	denotes	the
T16577	34910-34915	JJ	denotes	NF-κB
T16578	34916-34925	NNS	denotes	enhancers
T16579	34926-34928	IN	denotes	in
T16580	34929-34936	NN	denotes	absence
T16581	34937-34939	IN	denotes	of
T16582	34940-34943	NNP	denotes	Tat
T16583	34943-34944	,	denotes	,
T16584	34945-34948	CC	denotes	and
T16585	34949-34951	PRP	denotes	it
T16586	34952-34955	VBD	denotes	was
T16587	34956-34969	RB	denotes	significantly
T16588	34970-34977	VBN	denotes	removed
T16589	34978-34980	IN	denotes	in
T16590	34981-34989	NN	denotes	presence
T16591	34990-34992	IN	denotes	of
T16592	34993-34996	NNP	denotes	Tat
T16593	34996-34997	,	denotes	,
T16594	34998-35000	CC	denotes	or
T16595	35001-35010	VBG	denotes	following
T16596	35011-35016	NNP	denotes	IκB-α
T16597	35017-35020	NNP	denotes	RNA
T16598	35021-35033	NN	denotes	interference
T16599	35034-35035	-LRB-	denotes	(
T16600	35035-35041	NN	denotes	Figure
T16601	35042-35044	CD	denotes	5J
T16602	35044-35045	-RRB-	denotes	)
T16603	35045-35046	,	denotes	,
T16604	35047-35057	VBG	denotes	indicating
T16605	35058-35062	IN	denotes	that
T16606	35063-35066	NNP	denotes	Tat
T16607	35077-35082	NNP	denotes	IκB-α
T16608	35083-35087	IN	denotes	from
T16609	35088-35097	NNS	denotes	promoters
T16610	35097-35098	.	denotes	.
T18292	35100-35102	IN	denotes	In
T18293	35103-35117	JJ	denotes	HIV-1-infected
T18294	35118-35127	NNS	denotes	monocytes
T18295	35128-35131	NNP	denotes	Tat
T18296	35132-35140	VBZ	denotes	sustains
T18297	35141-35144	DT	denotes	the
T18298	35145-35150	JJ	denotes	NF-κB
T18299	35151-35159	NN	denotes	activity
T18300	35160-35163	CC	denotes	and
T18301	35164-35172	VBZ	denotes	promotes
T18302	35173-35176	DT	denotes	the
T18303	35177-35192	JJ	denotes	transcriptional
T18304	35193-35203	NN	denotes	activation
T18305	35204-35206	IN	denotes	of
T18306	35207-35213	NN	denotes	MIP-1α
T18307	35214-35216	IN	denotes	by
T18308	35217-35228	VBG	denotes	interacting
T18309	35229-35233	IN	denotes	with
T18310	35234-35239	NNP	denotes	IκB-α
T18311	35240-35243	CC	denotes	and
T18312	35244-35247	CD	denotes	p65
T18313	35248-35250	PRP	denotes	We
T18314	35251-35255	JJ	denotes	next
T18315	35256-35264	JJ	denotes	analysed
T18316	35265-35272	IN	denotes	whether
T18317	35273-35276	NNP	denotes	Tat
T18318	35277-35285	VBD	denotes	affected
T18319	35286-35289	DT	denotes	the
T18320	35290-35295	JJ	denotes	NF-κB
T18321	35296-35304	NN	denotes	activity
T18322	35305-35308	CC	denotes	and
T18323	35309-35312	DT	denotes	the
T18324	35313-35323	NN	denotes	expression
T18325	35324-35326	IN	denotes	of
T18326	35327-35333	NN	denotes	MIP-1α
T18327	35334-35336	IN	denotes	in
T18328	35337-35340	DT	denotes	the
T18329	35341-35347	NN	denotes	course
T18330	35348-35350	IN	denotes	of
T18331	35351-35356	JJ	denotes	HIV-1
T18332	35357-35366	NN	denotes	infection
T18333	35366-35367	.	denotes	.
T18334	35368-35370	TO	denotes	To
T18335	35371-35377	VB	denotes	detect
T18336	35378-35381	DT	denotes	the
T18337	35382-35385	NNP	denotes	Tat
T18338	35386-35393	NN	denotes	protein
T18339	35393-35394	,	denotes	,
T18340	35395-35397	PRP	denotes	we
T18341	35398-35406	VBD	denotes	produced
T18342	35407-35411	NNP	denotes	HXB2
T18343	35412-35427	NNP	denotes	Env-pseudotyped
T18344	35428-35433	NNP	denotes	NL4-3
T18345	35433-35434	.	denotes	.
T18346	35434-35442	NN	denotes	FLAG-Tat
T18347	35442-35443	.	denotes	.
T18348	35443-35444	NN	denotes	R
T18349	35444-35445	NN	denotes	−
T18350	35445-35446	NNP	denotes	E
T18351	35446-35447	CD	denotes	−
T18352	35448-35455	NNS	denotes	virions
T18353	35455-35456	,	denotes	,
T18354	35457-35462	WDT	denotes	which
T18355	35463-35467	VBD	denotes	were
T18356	35468-35472	VBN	denotes	used
T18357	35473-35475	IN	denotes	in
T18358	35476-35488	JJ	denotes	single-round
T18359	35489-35498	NN	denotes	infection
T18360	35499-35501	IN	denotes	of
T18361	35502-35506	NNP	denotes	U937
T18362	35506-35507	,	denotes	,
T18363	35508-35509	DT	denotes	a
T18364	35510-35515	JJ	denotes	human
T18365	35516-35525	JJ	denotes	monocytic
T18366	35526-35530	NN	denotes	cell
T18367	35531-35535	NN	denotes	line
T18368	35535-35536	.	denotes	.
T18369	35537-35542	NNS	denotes	Cells
T18370	35543-35547	VBD	denotes	were
T18371	35548-35559	VBN	denotes	transfected
T18372	35560-35564	IN	denotes	with
T18373	35565-35570	NNP	denotes	siRNA
T18374	35571-35574	NNP	denotes	Tat
T18375	35574-35575	,	denotes	,
T18376	35576-35581	NNP	denotes	siRNA
T18377	35582-35589	NN	denotes	control
T18378	35590-35592	CC	denotes	or
T18379	35593-35597	VBD	denotes	left
T18380	35598-35611	JJ	denotes	untransfected
T18381	35612-35614	TO	denotes	to
T18382	35615-35623	VB	denotes	modulate
T18383	35624-35627	DT	denotes	the
T18384	35628-35631	NNP	denotes	Tat
T18385	35632-35642	NN	denotes	expression
T18386	35642-35643	,	denotes	,
T18387	35644-35647	CC	denotes	and
T18388	35648-35652	RB	denotes	then
T18389	35653-35661	VBD	denotes	analysed
T18390	35662-35665	IN	denotes	for
T18391	35666-35669	DT	denotes	the
T18392	35670-35677	NN	denotes	kinetic
T18393	35678-35680	IN	denotes	of
T18394	35681-35686	JJ	denotes	NF-κB
T18395	35687-35697	NN	denotes	activation
T18396	35698-35707	VBG	denotes	following
T18397	35708-35713	JJ	denotes	viral
T18398	35714-35723	NN	denotes	infection
T18399	35723-35724	.	denotes	.
T18400	35725-35728	DT	denotes	The
T18401	35729-35739	NN	denotes	expression
T18402	35740-35742	IN	denotes	of
T18403	35743-35746	NNP	denotes	Tat
T18404	35747-35750	CC	denotes	and
T18405	35751-35754	CD	denotes	p24
T18406	35755-35758	VBD	denotes	was
T18407	35759-35767	VBN	denotes	detected
T18408	35768-35770	IN	denotes	at
T18409	35771-35772	LS	denotes	3
T18410	35772-35773	:	denotes	-
T18411	35774-35776	TO	denotes	to
T18412	35777-35781	JJ	denotes	12-h
T18413	35782-35796	NN	denotes	post-infection
T18414	35796-35797	,	denotes	,
T18415	35798-35803	IN	denotes	while
T18416	35804-35806	PRP	denotes	it
T18417	35807-35810	VBD	denotes	was
T18418	35811-35817	RB	denotes	barely
T18419	35818-35826	VBN	denotes	detected
T18420	35827-35829	IN	denotes	in
T18421	35830-35835	NNP	denotes	siRNA
T18422	35836-35851	VBD	denotes	Tat-transfected
T18423	35852-35857	NNS	denotes	cells
T18424	35858-35859	-LRB-	denotes	(
T18425	35859-35865	NN	denotes	Figure
T18426	35866-35868	NN	denotes	6A
T18427	35868-35869	-RRB-	denotes	)
T18428	35869-35870	.	denotes	.
T18429	38126-38129	DT	denotes	The
T18430	38130-38135	JJ	denotes	NF-κB
T18431	38136-38144	NN	denotes	activity
T18432	38144-38145	,	denotes	,
T18433	38146-38148	IN	denotes	as
T18434	38149-38157	VBN	denotes	measured
T18435	38158-38160	IN	denotes	by
T18436	38161-38164	CD	denotes	p65
T18437	38165-38168	NNP	denotes	DNA
T18438	38169-38176	JJ	denotes	binding
T18439	38176-38177	,	denotes	,
T18440	38178-38181	VBD	denotes	was
T18441	38182-38189	VBN	denotes	induced
T18442	38190-38192	IN	denotes	at
T18443	38193-38196	JJ	denotes	3-h
T18444	38197-38211	NN	denotes	post-infection
T18445	38212-38214	IN	denotes	in
T18446	38215-38219	DT	denotes	both
T18447	38220-38232	JJ	denotes	Tat-positive
T18448	38233-38234	-LRB-	denotes	(
T18449	38234-38236	DT	denotes	no
T18450	38237-38242	NN	denotes	siRNA
T18451	38243-38246	CC	denotes	and
T18452	38247-38252	NN	denotes	siRNA
T18453	38253-38260	NN	denotes	control
T18454	38260-38261	-RRB-	denotes	)
T18455	38262-38265	CC	denotes	and
T18456	38266-38278	JJ	denotes	Tat-negative
T18457	38279-38284	NNS	denotes	cells
T18458	38285-38286	-LRB-	denotes	(
T18459	38286-38291	NNP	denotes	siRNA
T18460	38292-38295	NNP	denotes	Tat
T18461	38295-38296	-RRB-	denotes	)
T18462	38296-38297	,	denotes	,
T18463	38298-38301	CC	denotes	and
T18464	38302-38311	VBD	denotes	increased
T18465	38312-38314	IN	denotes	at
T18466	38315-38317	CD	denotes	12
T18467	38318-38319	NN	denotes	h
T18468	38320-38324	RB	denotes	only
T18469	38325-38327	IN	denotes	in
T18470	38328-38340	JJ	denotes	Tat-positive
T18471	38341-38346	NNS	denotes	cells
T18472	38347-38348	-LRB-	denotes	(
T18473	38348-38354	NN	denotes	Figure
T18474	38355-38357	NN	denotes	6B
T18475	38357-38358	-RRB-	denotes	)
T18476	38358-38359	.	denotes	.
T18477	38360-38371	NN	denotes	Degradation
T18478	38372-38374	IN	denotes	of
T18479	38375-38380	NNP	denotes	IκB-α
T18480	38381-38384	VBD	denotes	was
T18481	38385-38393	VBN	denotes	observed
T18482	38394-38396	IN	denotes	at
T18483	38397-38400	JJ	denotes	3-h
T18484	38401-38415	NN	denotes	post-infection
T18485	38415-38416	,	denotes	,
T18486	38417-38420	CC	denotes	and
T18487	38421-38424	VBD	denotes	was
T18488	38425-38433	VBN	denotes	followed
T18489	38434-38436	IN	denotes	by
T18490	38437-38439	FW	denotes	de
T18491	38440-38444	FW	denotes	novo
T18492	38445-38454	NN	denotes	synthesis
T18493	38455-38457	IN	denotes	of
T18494	38458-38463	NNP	denotes	IκB-α
T18495	38464-38466	IN	denotes	at
T18496	38467-38469	CD	denotes	12
T18497	38470-38471	NN	denotes	h
T18498	38472-38485	RB	denotes	independently
T18499	38486-38488	IN	denotes	of
T18500	38489-38492	DT	denotes	the
T18501	38493-38496	NNP	denotes	Tat
T18502	38497-38505	NN	denotes	presence
T18503	38506-38507	-LRB-	denotes	(
T18504	38507-38513	NN	denotes	Figure
T18505	38514-38516	NN	denotes	6C
T18506	38516-38517	-RRB-	denotes	)
T18507	38517-38518	.	denotes	.
T18508	38519-38531	RB	denotes	Consistently
T18509	38531-38532	,	denotes	,
T18510	38533-38536	DT	denotes	the
T18511	38537-38540	NNP	denotes	IKK
T18512	38541-38549	NN	denotes	activity
T18513	38550-38553	VBD	denotes	was
T18514	38554-38561	VBN	denotes	induced
T18515	38562-38564	IN	denotes	at
T18516	38565-38568	JJ	denotes	3-h
T18517	38569-38583	NN	denotes	post-infection
T18518	38584-38587	CC	denotes	and
T18519	38588-38594	VBD	denotes	turned
T18520	38595-38598	RP	denotes	off
T18521	38599-38601	IN	denotes	at
T18522	38602-38604	CD	denotes	12
T18523	38605-38606	NN	denotes	h
T18524	38607-38620	RB	denotes	independently
T18525	38621-38623	IN	denotes	of
T18526	38624-38627	DT	denotes	the
T18527	38628-38631	NNP	denotes	Tat
T18528	38632-38640	NN	denotes	presence
T18529	38641-38642	-LRB-	denotes	(
T18530	38642-38648	NN	denotes	Figure
T18531	38649-38651	CD	denotes	6D
T18532	38651-38652	-RRB-	denotes	)
T18533	38652-38653	.	denotes	.
T18534	38654-38659	DT	denotes	These
T18535	38660-38667	NNS	denotes	results
T18536	38668-38674	VBD	denotes	agreed
T18537	38675-38679	IN	denotes	with
T18538	38680-38683	DT	denotes	the
T18539	38684-38691	NN	denotes	kinetic
T18540	38692-38694	IN	denotes	of
T18541	38695-38700	JJ	denotes	NF-κB
T18542	38701-38711	NN	denotes	activation
T18543	38712-38714	IN	denotes	in
T18544	38715-38727	JJ	denotes	single-round
T18545	38728-38733	JJ	denotes	HIV-1
T18546	38734-38743	NN	denotes	infection
T18547	38744-38746	IN	denotes	of
T18548	38747-38753	NNP	denotes	Jurkat
T18549	38754-38759	NNS	denotes	cells
T18550	38760-38761	-LRB-	denotes	(
T18551	38761-38767	NN	denotes	Figure
T18552	38768-38769	CD	denotes	1
T18553	38769-38770	-RRB-	denotes	)
T18554	38770-38771	,	denotes	,
T18555	38772-38775	CC	denotes	and
T18556	38776-38783	JJ	denotes	further
T18557	38784-38793	VBD	denotes	supported
T18558	38794-38797	DT	denotes	the
T18559	38798-38809	NN	denotes	requirement
T18560	38810-38812	IN	denotes	of
T18561	38813-38818	JJ	denotes	viral
T18562	38819-38829	NN	denotes	expression
T18563	38830-38832	TO	denotes	to
T18564	38833-38843	VB	denotes	counteract
T18565	38844-38847	DT	denotes	the
T18566	38848-38863	NN	denotes	post-activation
T18567	38864-38868	VB	denotes	turn
T18568	38869-38872	RB	denotes	off
T18569	38873-38875	IN	denotes	of
T18570	38876-38881	NN	denotes	NF-κB
T18571	38881-38882	.	denotes	.
T18572	38883-38885	IN	denotes	In
T18573	38886-38900	JJ	denotes	HIV-1-infected
T18574	38901-38905	NNP	denotes	U937
T18575	38906-38911	NNS	denotes	cells
T18576	38911-38912	,	denotes	,
T18577	38913-38916	DT	denotes	the
T18578	38917-38927	JJ	denotes	endogenous
T18579	38928-38931	NNP	denotes	Tat
T18580	38932-38935	VBD	denotes	was
T18581	38936-38956	VBN	denotes	coimmunoprecipitated
T18582	38957-38961	IN	denotes	with
T18583	38962-38967	NNP	denotes	IκB-α
T18584	38968-38971	CC	denotes	and
T18585	38972-38975	NNS	denotes	p65
T18586	38976-38977	-LRB-	denotes	(
T18587	38977-38983	NN	denotes	Figure
T18588	38984-38986	CD	denotes	6E
T18589	38986-38987	-RRB-	denotes	)
T18590	38987-38988	,	denotes	,
T18591	38989-38992	CC	denotes	and
T18592	38993-39002	VBD	denotes	activated
T18593	39003-39006	DT	denotes	the
T18594	39007-39013	JJ	denotes	MIP-1α
T18595	39014-39024	NN	denotes	expression
T18596	39025-39027	IN	denotes	in
T18597	39028-39029	DT	denotes	a
T18598	39030-39043	JJ	denotes	p65-dependent
T18599	39044-39050	NN	denotes	manner
T18600	39050-39051	,	denotes	,
T18601	39052-39054	IN	denotes	as
T18602	39055-39060	VBN	denotes	shown
T18603	39061-39063	IN	denotes	by
T18604	39064-39067	DT	denotes	the
T18605	39068-39072	NN	denotes	lack
T18606	39073-39075	IN	denotes	of
T18607	39076-39082	NN	denotes	effect
T18608	39083-39092	VBG	denotes	following
T18609	39093-39096	NNP	denotes	RNA
T18610	39097-39109	NN	denotes	interference
T18611	39110-39112	IN	denotes	of
T18612	39113-39116	NNP	denotes	Tat
T18613	39117-39119	CC	denotes	or
T18614	39120-39123	CD	denotes	p65
T18615	39124-39125	-LRB-	denotes	(
T18616	39125-39131	NN	denotes	Figure
T18617	39132-39134	CD	denotes	6F
T18618	39134-39135	-RRB-	denotes	)
T18619	39135-39136	.	denotes	.
T18620	39137-39145	RB	denotes	Moreover
T18621	39145-39146	,	denotes	,
T18622	39147-39150	NNP	denotes	Tat
T18623	39151-39154	CC	denotes	and
T18624	39155-39158	NNS	denotes	p65
T18625	39159-39163	VBD	denotes	were
T18626	39164-39168	DT	denotes	both
T18627	39169-39178	VBN	denotes	recruited
T18628	39179-39181	TO	denotes	to
T18629	39182-39185	DT	denotes	the
T18630	39186-39191	JJ	denotes	NF-κB
T18631	39192-39200	NN	denotes	enhancer
T18632	39201-39203	IN	denotes	of
T18633	39204-39210	NNP	denotes	MIP-1α
T18634	39210-39211	,	denotes	,
T18635	39212-39217	IN	denotes	while
T18636	39218-39221	DT	denotes	the
T18637	39222-39225	CD	denotes	p65
T18638	39226-39235	NN	denotes	occupancy
T18639	39236-39239	VBD	denotes	was
T18640	39240-39249	VBN	denotes	abolished
T18641	39250-39252	IN	denotes	by
T18642	39253-39258	NNP	denotes	siRNA
T18643	39259-39262	NNP	denotes	Tat
T18644	39263-39264	-LRB-	denotes	(
T18645	39264-39270	NNP	denotes	Figure
T18646	39271-39273	NNP	denotes	6G
T18647	39274-39277	CC	denotes	and
T18648	39278-39279	NNP	denotes	H
T18649	39279-39280	-RRB-	denotes	)
T18650	39280-39281	.	denotes	.
T18651	39282-39292	RB	denotes	Altogether
T18652	39293-39298	DT	denotes	these
T18653	39299-39306	NNS	denotes	results
T18654	39307-39316	VBD	denotes	indicated
T18655	39317-39321	IN	denotes	that
T18656	39322-39324	IN	denotes	in
T18657	39325-39331	JJ	denotes	single
T18658	39332-39337	NN	denotes	round
T18659	39338-39343	NN	denotes	HIV-1
T18660	39344-39353	NN	denotes	infection
T18661	39354-39357	NNP	denotes	Tat
T18662	39358-39368	VBN	denotes	associated
T18663	39369-39373	IN	denotes	with
T18664	39374-39379	NNP	denotes	IκB-α
T18665	39380-39383	CC	denotes	and
T18666	39384-39387	CD	denotes	p65
T18667	39387-39388	,	denotes	,
T18668	39389-39392	CC	denotes	and
T18669	39393-39400	VBD	denotes	induced
T18670	39401-39404	DT	denotes	the
T18671	39405-39418	JJ	denotes	p65-dependent
T18672	39419-39429	NN	denotes	activation
T18673	39430-39432	IN	denotes	of
T18674	39433-39439	JJ	denotes	MIP-1α
T18675	39440-39450	NN	denotes	expression
T18676	39451-39458	IN	denotes	through
T18677	39459-39468	NN	denotes	occupancy
T18678	39469-39471	IN	denotes	of
T18679	39472-39475	DT	denotes	the
T18680	39476-39482	JJ	denotes	MIP-1α
T18681	39483-39491	NN	denotes	promoter
T18682	39492-39495	CC	denotes	and
T18683	39496-39505	VBD	denotes	increased
T18684	39506-39517	NN	denotes	recruitment
T18685	39518-39520	IN	denotes	of
T18686	39521-39524	NNS	denotes	p65
T18687	39524-39525	.	denotes	.
T21240	39538-39542	DT	denotes	This
T21241	39543-39548	NN	denotes	study
T21242	39549-39556	VBZ	denotes	reports
T21243	39557-39558	DT	denotes	a
T21244	39559-39564	NN	denotes	novel
T21245	39565-39574	NN	denotes	mechanism
T21246	39575-39577	IN	denotes	of
T21247	39578-39583	JJ	denotes	NF-κB
T21248	39584-39594	NN	denotes	activation
T21249	39595-39597	IN	denotes	by
T21250	39598-39601	DT	denotes	the
T21251	39602-39607	NNP	denotes	HIV-1
T21252	39608-39611	NNP	denotes	Tat
T21253	39612-39626	NN	denotes	transactivator
T21254	39626-39627	.	denotes	.
T21255	39628-39633	VBN	denotes	Based
T21256	39634-39636	IN	denotes	on
T21257	39637-39640	DT	denotes	the
T21258	39641-39649	NN	denotes	evidence
T21259	39650-39654	IN	denotes	that
T21260	39655-39658	NNP	denotes	Tat
T21261	39659-39667	VBD	denotes	enhanced
T21262	39668-39671	DT	denotes	the
T21263	39672-39687	JJ	denotes	transcriptional
T21264	39688-39696	NN	denotes	activity
T21265	39697-39699	IN	denotes	of
T21266	39700-39703	DT	denotes	the
T21267	39704-39707	CD	denotes	p65
T21268	39708-39715	NN	denotes	subunit
T21269	39716-39718	IN	denotes	of
T21270	39719-39724	NNP	denotes	NF-κB
T21271	39725-39726	-LRB-	denotes	(
T21272	39726-39734	CD	denotes	49,63,64
T21273	39734-39735	-RRB-	denotes	)
T21274	39735-39736	,	denotes	,
T21275	39737-39740	CC	denotes	and
T21276	39741-39751	RB	denotes	physically
T21277	39752-39762	VBN	denotes	interacted
T21278	39763-39767	IN	denotes	with
T21279	39768-39771	DT	denotes	the
T21280	39772-39777	JJ	denotes	IκB-α
T21281	39778-39787	NN	denotes	repressor
T21282	39788-39789	-LRB-	denotes	(
T21283	39789-39794	CD	denotes	50,51
T21284	39794-39795	-RRB-	denotes	)
T21285	39795-39796	,	denotes	,
T21286	39797-39799	PRP	denotes	we
T21287	39800-39812	VBD	denotes	investigated
T21288	39813-39816	DT	denotes	the
T21289	39817-39828	NN	denotes	possibility
T21290	39829-39833	WDT	denotes	that
T21291	39834-39837	NNP	denotes	Tat
T21292	39838-39843	MD	denotes	could
T21293	39844-39852	VB	denotes	activate
T21294	39853-39858	NNP	denotes	NF-κB
T21295	39859-39862	IN	denotes	via
T21296	39863-39869	JJ	denotes	direct
T21297	39870-39881	NN	denotes	interaction
T21298	39882-39886	IN	denotes	with
T21299	39887-39892	NNP	denotes	IκB-α
T21300	39893-39896	CC	denotes	and
T21301	39897-39900	NNS	denotes	p65
T21302	39900-39901	.	denotes	.
T21303	39902-39904	TO	denotes	To
T21304	39905-39909	DT	denotes	this
T21305	39910-39913	NN	denotes	end
T21306	39913-39914	,	denotes	,
T21307	39915-39918	DT	denotes	the
T21308	39919-39924	NN	denotes	NF-κB
T21309	39925-39933	NN	denotes	activity
T21310	39934-39937	VBD	denotes	was
T21311	39938-39947	VBN	denotes	monitored
T21312	39948-39950	IN	denotes	in
T21313	39951-39957	JJ	denotes	single
T21314	39958-39963	NN	denotes	round
T21315	39964-39969	NN	denotes	HIV-1
T21316	39970-39979	NN	denotes	infection
T21317	39980-39985	VBG	denotes	using
T21318	39986-39989	NNP	denotes	RNA
T21319	39990-40002	NN	denotes	interference
T21320	40003-40005	TO	denotes	to
T21321	40006-40013	NN	denotes	silence
T21322	40014-40017	DT	denotes	the
T21323	40018-40021	NNP	denotes	Tat
T21324	40022-40032	NN	denotes	expression
T21325	40032-40033	.	denotes	.
T21326	40034-40036	IN	denotes	By
T21327	40037-40041	DT	denotes	this
T21328	40042-40050	NN	denotes	approach
T21329	40050-40051	,	denotes	,
T21330	40052-40054	PRP	denotes	we
T21331	40055-40062	VBD	denotes	avoided
T21332	40063-40066	DT	denotes	the
T21333	40067-40079	NN	denotes	perpetuation
T21334	40080-40082	IN	denotes	of
T21335	40083-40088	JJ	denotes	NF-κB
T21336	40089-40099	NN	denotes	activation
T21337	40100-40109	VBG	denotes	signaling
T21338	40110-40113	JJ	denotes	due
T21339	40114-40116	TO	denotes	to
T21340	40117-40127	JJ	denotes	subsequent
T21341	40128-40134	NNS	denotes	rounds
T21342	40135-40137	IN	denotes	of
T21343	40138-40143	JJ	denotes	viral
T21344	40144-40149	NN	denotes	entry
T21345	40150-40152	IN	denotes	in
T21346	40153-40157	NN	denotes	cell
T21347	40158-40165	NN	denotes	culture
T21348	40166-40177	NN	denotes	propagation
T21349	40178-40179	-LRB-	denotes	(
T21350	40179-40184	CD	denotes	30,31
T21351	40184-40185	-RRB-	denotes	)
T21352	40185-40186	.	denotes	.
T21353	40187-40191	IN	denotes	Upon
T21354	40192-40197	JJ	denotes	HIV-1
T21355	40198-40207	NN	denotes	infection
T21356	40207-40208	,	denotes	,
T21357	40209-40212	DT	denotes	the
T21358	40213-40218	JJ	denotes	early
T21359	40219-40224	NN	denotes	NF-κB
T21360	40225-40235	NN	denotes	activation
T21361	40236-40244	VBD	denotes	occurred
T21362	40245-40258	RB	denotes	concomitantly
T21363	40259-40263	IN	denotes	with
T21364	40264-40267	NNP	denotes	IKK
T21365	40268-40278	NN	denotes	activation
T21366	40279-40282	CC	denotes	and
T21367	40283-40288	JJ	denotes	IκB-α
T21368	40289-40300	NN	denotes	degradation
T21369	40301-40303	IN	denotes	in
T21370	40304-40307	DT	denotes	the
T21371	40308-40315	NN	denotes	absence
T21372	40316-40318	IN	denotes	of
T21373	40319-40322	NNP	denotes	Tat
T21374	40322-40323	.	denotes	.
T21375	40324-40328	RB	denotes	Soon
T21376	40329-40334	IN	denotes	after
T21377	40335-40338	DT	denotes	the
T21378	40339-40343	VBN	denotes	shut
T21379	40344-40347	IN	denotes	off
T21380	40348-40350	IN	denotes	of
T21381	40351-40354	NNP	denotes	IKK
T21382	40355-40363	NN	denotes	activity
T21383	40364-40367	CC	denotes	and
T21384	40368-40371	JJ	denotes	new
T21385	40372-40381	NN	denotes	synthesis
T21386	40382-40384	IN	denotes	of
T21387	40385-40390	NNP	denotes	IκB-α
T21388	40390-40391	,	denotes	,
T21389	40392-40395	DT	denotes	the
T21390	40396-40401	NN	denotes	NF-κB
T21391	40402-40410	NN	denotes	activity
T21392	40411-40414	VBD	denotes	was
T21393	40415-40419	VBN	denotes	kept
T21394	40420-40428	VBN	denotes	elevated
T21395	40429-40431	IN	denotes	in
T21396	40432-40435	DT	denotes	the
T21397	40436-40444	NN	denotes	presence
T21398	40445-40447	IN	denotes	of
T21399	40448-40451	NNP	denotes	Tat
T21400	40451-40452	,	denotes	,
T21401	40453-40458	IN	denotes	while
T21402	40459-40461	PRP	denotes	it
T21403	40462-40465	VBD	denotes	was
T21404	40466-40470	RB	denotes	down
T21405	40471-40480	VBN	denotes	regulated
T21406	40481-40485	IN	denotes	upon
T21407	40486-40495	VBG	denotes	silencing
T21408	40496-40498	IN	denotes	of
T21409	40499-40502	DT	denotes	the
T21410	40503-40506	NNP	denotes	Tat
T21411	40507-40511	NN	denotes	gene
T21412	40511-40512	.	denotes	.
T21413	40513-40518	DT	denotes	These
T21414	40519-40527	NNS	denotes	findings
T21415	40528-40536	VBP	denotes	indicate
T21416	40537-40541	IN	denotes	that
T21417	40542-40544	IN	denotes	in
T21418	40545-40551	JJ	denotes	single
T21419	40552-40557	NN	denotes	round
T21420	40558-40563	NN	denotes	HIV-1
T21421	40564-40573	NN	denotes	infection
T21422	40573-40574	,	denotes	,
T21423	40575-40578	NNP	denotes	Tat
T21424	40579-40587	VBD	denotes	enhanced
T21425	40588-40591	DT	denotes	the
T21426	40592-40597	JJ	denotes	NF-κB
T21427	40598-40606	NN	denotes	activity
T21428	40607-40614	IN	denotes	without
T21429	40615-40624	VBG	denotes	affecting
T21430	40625-40628	DT	denotes	the
T21431	40629-40632	NNP	denotes	IKK
T21432	40633-40641	NN	denotes	activity
T21433	40642-40645	CC	denotes	and
T21434	40646-40649	DT	denotes	the
T21435	40650-40659	NN	denotes	half-life
T21436	40660-40662	IN	denotes	of
T21437	40663-40668	NNP	denotes	IκB-α
T21438	40668-40669	.	denotes	.
T21439	40670-40677	JJ	denotes	Similar
T21440	40678-40685	NN	denotes	kinetic
T21441	40686-40688	IN	denotes	of
T21442	40689-40694	JJ	denotes	NF-κB
T21443	40695-40705	NN	denotes	activation
T21444	40706-40714	VBD	denotes	occurred
T21445	40715-40719	IN	denotes	upon
T21446	40720-40731	NN	denotes	short-pulse
T21447	40732-40734	IN	denotes	of
T21448	40735-40738	NNP	denotes	PMA
T21449	40739-40741	IN	denotes	in
T21450	40742-40757	NNP	denotes	Tat-transfected
T21451	40758-40762	NNP	denotes	HeLa
T21452	40763-40768	NNS	denotes	cells
T21453	40768-40769	,	denotes	,
T21454	40770-40775	WRB	denotes	where
T21455	40776-40779	NNP	denotes	Tat
T21456	40780-40789	VBD	denotes	inhibited
T21457	40790-40793	DT	denotes	the
T21458	40794-40809	JJ	denotes	post-activation
T21459	40810-40814	NN	denotes	turn
T21460	40815-40818	IN	denotes	off
T21461	40819-40821	IN	denotes	of
T21462	40822-40827	NN	denotes	NF-κB
T21463	40828-40830	IN	denotes	in
T21464	40831-40839	NN	denotes	presence
T21465	40840-40842	IN	denotes	of
T21466	40843-40848	RB	denotes	newly
T21467	40849-40860	VBN	denotes	synthesized
T21468	40861-40866	NNP	denotes	IκB-α
T21469	40866-40867	.	denotes	.
T21470	40868-40871	DT	denotes	The
T21471	40872-40885	JJ	denotes	Tat-dependent
T21472	40886-40896	NN	denotes	activation
T21473	40897-40899	IN	denotes	of
T21474	40900-40905	JJ	denotes	NF-κB
T21475	40906-40916	VBN	denotes	correlated
T21476	40917-40921	IN	denotes	with
T21477	40922-40925	DT	denotes	the
T21478	40926-40937	NN	denotes	association
T21479	40938-40940	IN	denotes	of
T21480	40941-40944	DT	denotes	the
T21481	40945-40950	JJ	denotes	viral
T21482	40951-40958	NN	denotes	protein
T21483	40959-40963	IN	denotes	with
T21484	40964-40969	NNP	denotes	IκB-α
T21485	40969-40970	,	denotes	,
T21486	40971-40976	WDT	denotes	which
T21487	40977-40987	VBD	denotes	interfered
T21488	40988-40992	IN	denotes	with
T21489	40993-40996	DT	denotes	the
T21490	40997-41007	NN	denotes	generation
T21491	41008-41010	IN	denotes	of
T21492	41011-41014	DT	denotes	the
T21493	41015-41020	NNP	denotes	IκB-α
T21494	41020-41021	NNP	denotes	/
T21495	41021-41024	CD	denotes	p65
T21496	41025-41032	NN	denotes	complex
T21497	41032-41033	.	denotes	.
T21498	41034-41038	DT	denotes	This
T21499	41039-41047	NN	denotes	evidence
T21500	41048-41060	VBD	denotes	demonstrated
T21501	41061-41065	IN	denotes	that
T21502	41066-41069	NNP	denotes	Tat
T21503	41070-41078	VBD	denotes	competed
T21504	41079-41082	IN	denotes	for
T21505	41083-41086	DT	denotes	the
T21506	41087-41094	JJ	denotes	binding
T21507	41095-41097	IN	denotes	of
T21508	41098-41103	NNP	denotes	IκB-α
T21509	41104-41106	TO	denotes	to
T21510	41107-41110	VB	denotes	p65
T21511	41111-41114	CC	denotes	and
T21512	41115-41124	VBD	denotes	prevented
T21513	41125-41128	DT	denotes	the
T21514	41129-41134	JJ	denotes	IκB-α
T21515	41135-41145	NN	denotes	repression
T21516	41146-41148	IN	denotes	of
T21517	41149-41152	NNS	denotes	p65
T21518	41152-41153	.	denotes	.
T21519	41154-41156	IN	denotes	As
T21520	41157-41167	JJ	denotes	additional
T21521	41168-41177	NN	denotes	mechanism
T21522	41178-41180	IN	denotes	of
T21523	41181-41186	JJ	denotes	NF-κB
T21524	41187-41197	NN	denotes	activation
T21525	41197-41198	,	denotes	,
T21526	41199-41202	NNP	denotes	Tat
T21527	41203-41213	VBN	denotes	associated
T21528	41214-41218	IN	denotes	with
T21529	41219-41222	NNS	denotes	p65
T21530	41223-41226	CC	denotes	and
T21531	41227-41236	VBD	denotes	increased
T21532	41237-41240	DT	denotes	the
T21533	41241-41244	CD	denotes	p65
T21534	41245-41252	JJ	denotes	binding
T21535	41253-41261	NN	denotes	affinity
T21536	41262-41264	TO	denotes	to
T21537	41265-41268	DT	denotes	the
T21538	41269-41274	JJ	denotes	NF-κB
T21539	41275-41283	NN	denotes	enhancer
T21540	41283-41284	:	denotes	;
T21541	41285-41289	DT	denotes	this
T21542	41290-41298	NN	denotes	evidence
T21543	41299-41302	VBD	denotes	was
T21544	41303-41311	VBN	denotes	obtained
T21545	41312-41316	IN	denotes	with
T21546	41317-41328	JJ	denotes	recombinant
T21547	41329-41337	NNS	denotes	proteins
T21548	41338-41348	VBG	denotes	indicating
T21549	41349-41353	IN	denotes	that
T21550	41354-41357	DT	denotes	the
T21551	41358-41366	JJR	denotes	stronger
T21552	41367-41374	JJ	denotes	binding
T21553	41375-41377	IN	denotes	of
T21554	41378-41381	CD	denotes	p65
T21555	41382-41384	TO	denotes	to
T21556	41385-41388	NNP	denotes	DNA
T21557	41389-41392	VBD	denotes	was
T21558	41393-41394	DT	denotes	a
T21559	41395-41406	NN	denotes	consequence
T21560	41407-41409	IN	denotes	of
T21561	41410-41416	JJ	denotes	direct
T21562	41417-41428	NN	denotes	association
T21563	41429-41433	IN	denotes	with
T21564	41434-41437	NNP	denotes	Tat
T21565	41437-41438	,	denotes	,
T21566	41439-41444	WDT	denotes	which
T21567	41445-41451	RB	denotes	likely
T21568	41452-41458	VBD	denotes	caused
T21569	41459-41460	DT	denotes	a
T21570	41461-41475	JJ	denotes	conformational
T21571	41476-41482	NN	denotes	change
T21572	41483-41485	IN	denotes	of
T21573	41486-41489	NNS	denotes	p65
T21574	41489-41490	.	denotes	.
T21575	41491-41493	PRP	denotes	We
T21576	41494-41498	RB	denotes	also
T21577	41499-41511	VBD	denotes	demonstrated
T21578	41512-41516	IN	denotes	that
T21579	41517-41520	DT	denotes	the
T21580	41521-41524	NNP	denotes	Tat
T21581	41525-41538	NN	denotes	cysteine-rich
T21582	41539-41547	NN	denotes	sequence
T21583	41548-41549	-LRB-	denotes	(
T21584	41549-41552	CD	denotes	C22
T21585	41552-41558	CD	denotes	,25,27
T21586	41558-41559	-RRB-	denotes	)
T21587	41560-41563	VBD	denotes	was
T21588	41564-41572	VBN	denotes	involved
T21589	41573-41575	IN	denotes	in
T21590	41576-41579	DT	denotes	the
T21591	41580-41587	JJ	denotes	binding
T21592	41588-41590	TO	denotes	to
T21593	41591-41594	DT	denotes	the
T21594	41595-41598	NNP	denotes	RHD
T21595	41599-41601	IN	denotes	of
T21596	41602-41605	CD	denotes	p65
T21597	41606-41609	CC	denotes	and
T21598	41610-41615	NNP	denotes	NF-κB
T21599	41616-41626	NN	denotes	activation
T21600	41626-41627	.	denotes	.
T21601	41628-41639	RB	denotes	Differently
T21602	41639-41640	,	denotes	,
T21603	41641-41644	DT	denotes	the
T21604	41645-41648	NNP	denotes	Tat
T21605	41649-41662	JJ	denotes	arginine-rich
T21606	41663-41671	NN	denotes	sequence
T21607	41672-41673	-LRB-	denotes	(
T21608	41673-41676	CD	denotes	R49
T21609	41676-41691	CD	denotes	,52,53,55,56,57
T21610	41691-41692	-RRB-	denotes	)
T21611	41693-41696	VBD	denotes	was
T21612	41697-41705	VBN	denotes	required
T21613	41706-41709	IN	denotes	for
T21614	41710-41713	DT	denotes	the
T21615	41714-41721	JJ	denotes	binding
T21616	41722-41724	TO	denotes	to
T21617	41725-41728	DT	denotes	the
T21618	41729-41734	JJ	denotes	sixth
T21619	41735-41742	NN	denotes	ankyrin
T21620	41743-41745	IN	denotes	of
T21621	41746-41751	NNP	denotes	IκB-α
T21622	41752-41753	-LRB-	denotes	(
T21623	41753-41758	CD	denotes	50,51
T21624	41758-41759	-RRB-	denotes	)
T21625	41759-41760	,	denotes	,
T21626	41761-41764	CC	denotes	and
T21627	41765-41768	IN	denotes	for
T21628	41769-41778	VBG	denotes	releasing
T21673	42043-42053	NN	denotes	activation
T21674	42054-42056	IN	denotes	of
T21675	42057-42062	JJ	denotes	NF-κB
T21676	42063-42069	VBD	denotes	showed
T21677	42070-42074	IN	denotes	that
T21678	42075-42084	JJ	denotes	wild-type
T21679	42085-42088	NNP	denotes	Tat
T21680	42088-42089	,	denotes	,
T21681	42090-42093	CC	denotes	and
T21682	42094-42097	RB	denotes	not
T21683	42098-42101	DT	denotes	the
T21684	42102-42105	NNP	denotes	Tat
T21685	42106-42113	NNS	denotes	mutants
T21686	42114-42121	VBG	denotes	lacking
T21687	42122-42125	DT	denotes	the
T21688	42126-42134	NN	denotes	arginine
T21689	42134-42135	:	denotes	-
T21690	42136-42138	CC	denotes	or
T21691	42139-42152	JJ	denotes	cysteine-rich
T21692	42153-42160	NNS	denotes	domains
T21693	42160-42161	,	denotes	,
T21694	42162-42174	JJ	denotes	up-regulated
T21695	42175-42177	IN	denotes	in
T21696	42178-42182	NN	denotes	vivo
T21697	42183-42186	DT	denotes	the
T21698	42187-42197	NN	denotes	expression
T21699	42198-42200	IN	denotes	of
T21700	42201-42202	DT	denotes	a
T21701	42203-42209	NN	denotes	number
T21702	42210-42212	IN	denotes	of
T21703	42213-42229	JJ	denotes	NF-κB-responsive
T21704	42230-42235	NNS	denotes	genes
T21705	42235-42236	,	denotes	,
T21706	42237-42246	VBG	denotes	including
T21707	42247-42253	NNP	denotes	MIP-1α
T21708	42253-42254	,	denotes	,
T21709	42255-42259	NNP	denotes	CSF3
T21710	42259-42260	,	denotes	,
T21711	42261-42264	NNP	denotes	LTA
T21712	42264-42265	,	denotes	,
T21713	42266-42272	NNP	denotes	NFKBIA
T21714	42273-42276	CC	denotes	and
T21715	42277-42281	NNP	denotes	TLR2
T21716	42281-42282	.	denotes	.
T21717	42283-42285	IN	denotes	By
T21718	42286-42290	NNP	denotes	ChIP
T21719	42291-42299	NN	denotes	analysis
T21720	42299-42300	,	denotes	,
T21721	42301-42303	PRP	denotes	we
T21722	42304-42312	VBD	denotes	observed
T21723	42313-42317	IN	denotes	that
T21724	42318-42321	NNP	denotes	Tat
T21725	42322-42331	VBD	denotes	increased
T21726	42332-42335	DT	denotes	the
T21727	42336-42347	NN	denotes	recruitment
T21728	42348-42350	IN	denotes	of
T21729	42351-42354	CD	denotes	p65
T21730	42355-42357	TO	denotes	to
T21731	42358-42361	DT	denotes	the
T21732	42362-42367	JJ	denotes	NF-κB
T21733	42368-42377	NNS	denotes	enhancers
T21734	42378-42380	IN	denotes	of
T21735	42381-42384	DT	denotes	the
T21736	42385-42394	VBN	denotes	activated
T21737	42395-42400	NNS	denotes	genes
T21738	42400-42401	,	denotes	,
T21739	42402-42405	CC	denotes	and
T21740	42406-42409	VBD	denotes	was
T21741	42410-42419	VBN	denotes	recovered
T21742	42420-42422	IN	denotes	at
T21743	42423-42426	DT	denotes	the
T21744	42427-42431	JJ	denotes	same
T21745	42432-42437	NNS	denotes	sites
T21746	42437-42438	.	denotes	.
T21747	42439-42442	NNP	denotes	RNA
T21748	42443-42455	NN	denotes	interference
T21749	42456-42458	IN	denotes	of
T21750	42459-42462	CD	denotes	p65
T21751	42463-42472	VBD	denotes	abolished
T21752	42473-42476	DT	denotes	the
T21753	42477-42480	NNP	denotes	Tat
T21754	42481-42490	NN	denotes	occupancy
T21755	42491-42493	IN	denotes	of
T21756	42494-42497	DT	denotes	the
T21757	42498-42503	JJ	denotes	NF-κB
T21758	42504-42513	NNS	denotes	enhancers
T21759	42514-42524	VBG	denotes	indicating
T21760	42525-42529	IN	denotes	that
T21761	42530-42533	DT	denotes	the
T21762	42534-42537	NNP	denotes	Tat
T21763	42538-42545	JJ	denotes	binding
T21764	42546-42548	TO	denotes	to
T21765	42549-42552	DT	denotes	the
T21766	42553-42558	JJ	denotes	NF-κB
T21767	42559-42564	NNS	denotes	sites
T21768	42565-42568	VBD	denotes	was
T21769	42569-42577	VBN	denotes	mediated
T21770	42578-42580	IN	denotes	by
T21771	42581-42584	NNS	denotes	p65
T21772	42584-42585	.	denotes	.
T21773	42586-42591	DT	denotes	These
T21774	42592-42599	NNS	denotes	results
T21775	42600-42604	VBD	denotes	were
T21776	42605-42615	JJ	denotes	consistent
T21777	42616-42620	IN	denotes	with
T21778	42621-42624	DT	denotes	the
T21779	42625-42633	NN	denotes	evidence
T21780	42634-42638	IN	denotes	that
T21781	42639-42642	NNP	denotes	Tat
T21782	42643-42652	VBD	denotes	displaced
T21783	42653-42656	CD	denotes	p65
T21784	42657-42661	IN	denotes	from
T21785	42662-42665	DT	denotes	the
T21786	42666-42673	JJ	denotes	binding
T21787	42674-42676	TO	denotes	to
T21788	42677-42682	NNP	denotes	IκB-α
T21789	42683-42684	-LRB-	denotes	(
T21790	42684-42691	NNS	denotes	Figures
T21791	42692-42694	JJ	denotes	2D
T21792	42695-42698	CC	denotes	and
T21793	42699-42701	NNP	denotes	3C
T21794	42701-42702	-RRB-	denotes	)
T21795	42702-42703	,	denotes	,
T21796	42704-42707	CC	denotes	and
T21797	42708-42717	VBD	denotes	increased
T21798	42718-42721	DT	denotes	the
T21799	42722-42725	CD	denotes	p65
T21800	42726-42729	NNP	denotes	DNA
T21801	42730-42737	JJ	denotes	binding
T21802	42738-42746	NN	denotes	affinity
T21803	42747-42754	IN	denotes	through
T21804	42755-42766	NN	denotes	association
T21805	42767-42768	-LRB-	denotes	(
T21806	42768-42774	NN	denotes	Figure
T21807	42775-42777	CD	denotes	4E
T21808	42778-42781	CC	denotes	and
T21809	42782-42783	NN	denotes	F
T21810	42783-42784	-RRB-	denotes	)
T21811	42784-42785	.	denotes	.
T21812	42786-42790	RB	denotes	Thus
T21813	42790-42791	,	denotes	,
T21814	42792-42795	NNP	denotes	Tat
T21815	42796-42802	JJ	denotes	likely
T21816	42803-42812	VBD	denotes	increased
T21817	42813-42816	DT	denotes	the
T21818	42817-42828	NN	denotes	recruitment
T21819	42829-42831	IN	denotes	of
T21820	42832-42835	CD	denotes	p65
T21821	42836-42838	TO	denotes	to
T21822	42839-42842	DT	denotes	the
T21823	42843-42858	JJ	denotes	NF-κB-dependent
T21824	42859-42868	NNS	denotes	promoters
T21825	42869-42871	IN	denotes	by
T21826	42872-42883	VBG	denotes	associating
T21827	42884-42888	IN	denotes	with
T21828	42889-42892	NNS	denotes	p65
T21829	42893-42896	CC	denotes	and
T21830	42897-42899	IN	denotes	by
T21831	42900-42911	VBG	denotes	interfering
T21832	42912-42916	IN	denotes	with
T21833	42917-42920	DT	denotes	the
T21834	42921-42929	NN	denotes	assembly
T21835	42930-42932	IN	denotes	of
T21836	42933-42936	NNS	denotes	p65
T21837	42937-42941	IN	denotes	with
T21838	42942-42945	DT	denotes	the
T21839	42946-42955	NN	denotes	repressor
T21840	42956-42961	NN	denotes	IκB-α
T21841	42961-42962	.	denotes	.
T21842	42963-42965	IN	denotes	Of
T21843	42966-42974	NN	denotes	interest
T21844	42974-42975	,	denotes	,
T21845	42976-42981	NNP	denotes	IκB-α
T21846	42982-42985	VBD	denotes	was
T21847	42986-42991	VBN	denotes	found
T21848	42992-43002	VBN	denotes	associated
T21849	43003-43005	TO	denotes	to
T21850	43006-43009	DT	denotes	the
T21851	43010-43015	JJ	denotes	NF-κB
T21852	43016-43025	NNS	denotes	enhancers
T21853	43026-43028	IN	denotes	in
T21854	43029-43036	NN	denotes	absence
T21855	43037-43039	IN	denotes	of
T21856	43040-43043	NNP	denotes	Tat
T21857	43043-43044	,	denotes	,
T21858	43045-43050	WRB	denotes	where
T21859	43051-43053	PRP	denotes	it
T21860	43054-43057	VBD	denotes	was
T21861	43058-43067	VBN	denotes	displaced
T21862	43068-43070	IN	denotes	in
T21863	43071-43079	NN	denotes	presence
T21864	43080-43082	IN	denotes	of
T21865	43083-43086	NNP	denotes	Tat
T21866	43086-43087	.	denotes	.
T21867	43088-43093	DT	denotes	These
T21868	43094-43101	NNS	denotes	results
T21869	43102-43109	VBP	denotes	suggest
T21870	43110-43111	DT	denotes	a
T21871	43112-43120	JJ	denotes	negative
T21872	43121-43131	JJ	denotes	regulatory
T21873	43132-43136	NN	denotes	role
T21874	43137-43139	IN	denotes	of
T21875	43140-43145	NNP	denotes	IκB-α
T21876	43146-43148	IN	denotes	in
T21877	43149-43153	NN	denotes	gene
T21878	43154-43164	NN	denotes	regulation
T21879	43165-43172	IN	denotes	through
T21880	43173-43182	NN	denotes	occupancy
T21881	43183-43185	IN	denotes	of
T21882	43186-43194	JJ	denotes	specific
T21883	43195-43204	NNS	denotes	promoters
T21884	43204-43205	.	denotes	.
T21885	43206-43212	RB	denotes	Indeed
T21886	43212-43213	,	denotes	,
T21887	43214-43219	NNP	denotes	IκB-α
T21888	43220-43223	VBD	denotes	was
T21889	43224-43229	VBN	denotes	found
T21890	43230-43240	VBN	denotes	associated
T21891	43241-43245	IN	denotes	with
T21892	43246-43253	NN	denotes	hystone
T21893	43254-43266	NNS	denotes	deacetylases
T21894	43267-43269	IN	denotes	at
T21895	43270-43273	DT	denotes	the
T21896	43274-43278	CD	denotes	hes1
T21897	43279-43287	NN	denotes	promoter
T21898	43287-43288	,	denotes	,
T21899	43289-43293	IN	denotes	from
T21900	43294-43299	WRB	denotes	where
T21901	43300-43302	PRP	denotes	it
T21902	43303-43306	VBD	denotes	was
T21903	43307-43314	VBN	denotes	removed
T21904	43315-43324	VBG	denotes	following
T21905	43325-43330	JJ	denotes	TNF-α
T21906	43331-43342	NN	denotes	stimulation
T21907	43343-43350	VBG	denotes	causing
T21908	43351-43358	NN	denotes	histone
T21909	43359-43370	NN	denotes	acetylation
T21910	43371-43374	CC	denotes	and
T21911	43375-43379	CD	denotes	hes1
T21912	43380-43395	JJ	denotes	transcriptional
T21913	43396-43406	NN	denotes	activation
T21914	43407-43408	-LRB-	denotes	(
T21915	43408-43410	CD	denotes	65
T21916	43410-43411	-RRB-	denotes	)
T21917	43411-43412	.	denotes	.
T21918	43413-43414	DT	denotes	A
T21919	43415-43422	JJ	denotes	similar
T21920	43423-43432	NN	denotes	mechanism
T21921	43433-43435	IN	denotes	of
T21922	43436-43440	NN	denotes	gene
T21923	43441-43451	NN	denotes	regulation
T21924	43452-43457	MD	denotes	could
T21925	43458-43463	VB	denotes	apply
T21926	43464-43466	TO	denotes	to
T21927	43467-43470	DT	denotes	the
T21928	43471-43486	JJ	denotes	NF-κB-dependent
T21929	43487-43492	NNS	denotes	genes
T21930	43493-43501	VBN	denotes	analysed
T21931	43502-43504	IN	denotes	in
T21932	43505-43509	DT	denotes	this
T21933	43510-43515	NN	denotes	study
T21934	43515-43516	,	denotes	,
T21935	43517-43522	WRB	denotes	where
T21936	43523-43526	NNP	denotes	Tat
T21937	43527-43532	MD	denotes	could
T21938	43533-43541	VB	denotes	activate
T21939	43542-43545	DT	denotes	the
T21940	43546-43550	NN	denotes	gene
T21941	43551-43561	NN	denotes	expression
T21942	43562-43564	IN	denotes	by
T21943	43565-43573	VBG	denotes	removing
T21944	43574-43579	NNP	denotes	IκB-α
T21945	43580-43583	CC	denotes	and
T21946	43584-43593	VBG	denotes	promoting
T21947	43594-43597	DT	denotes	the
T21948	43598-43601	CD	denotes	p65
T21949	43602-43609	VBG	denotes	loading
T21950	43610-43611	-LRB-	denotes	(
T21951	43611-43617	NN	denotes	Figure
T21952	43618-43619	CD	denotes	7
T21953	43619-43620	-RRB-	denotes	)
T21954	43620-43621	.	denotes	.
T21955	44045-44048	DT	denotes	The
T21956	44049-44062	JJ	denotes	physiological
T21957	44063-44072	NN	denotes	relevance
T21958	44073-44075	IN	denotes	of
T21959	44076-44079	NNP	denotes	Tat
T21960	44080-44085	VBP	denotes	cross
T21961	44086-44090	NN	denotes	talk
T21962	44091-44095	IN	denotes	with
T21963	44096-44101	NN	denotes	NF-κB
T21964	44102-44105	VBD	denotes	was
T21965	44106-44118	VBN	denotes	demonstrated
T21966	44119-44121	IN	denotes	in
T21967	44122-44125	DT	denotes	the
T21968	44126-44131	NNP	denotes	HIV-1
T21969	44132-44141	NN	denotes	infection
T21970	44142-44144	IN	denotes	of
T21971	44145-44150	JJ	denotes	human
T21972	44151-44160	NNS	denotes	monocytes
T21973	44160-44161	,	denotes	,
T21974	44162-44167	WRB	denotes	where
T21975	44168-44181	JJ	denotes	HIV-1-encoded
T21976	44182-44185	NNP	denotes	Tat
T21977	44186-44193	NN	denotes	protein
T21978	44194-44206	VBD	denotes	counteracted
T21979	44207-44222	JJ	denotes	post-activation
T21980	44223-44227	NN	denotes	turn
T21981	44228-44231	IN	denotes	off
T21982	44232-44234	IN	denotes	of
T21983	44235-44240	NNP	denotes	NF-κB
T21984	44240-44241	,	denotes	,
T21985	44242-44244	IN	denotes	as
T21986	44245-44250	VBN	denotes	shown
T21987	44251-44253	IN	denotes	by
T21988	44253-44254	:	denotes	:
T21989	44255-44256	-LRB-	denotes	(
T21990	44256-44257	FW	denotes	i
T21991	44257-44258	-RRB-	denotes	)
T21992	44259-44268	VBD	denotes	sustained
T21993	44269-44274	JJ	denotes	NF-κB
T21994	44275-44283	NN	denotes	activity
T21995	44284-44286	IN	denotes	by
T21996	44287-44290	NNP	denotes	Tat
T21997	44291-44293	IN	denotes	in
T21998	44294-44302	NN	denotes	presence
T21999	44303-44305	IN	denotes	of
T22000	44306-44311	RB	denotes	newly
T22001	44312-44323	VBN	denotes	synthesized
T22002	44324-44329	NNP	denotes	IκB-α
T22003	44329-44330	:	denotes	;
T22004	44331-44332	-LRB-	denotes	(
T22005	44332-44334	FW	denotes	ii
T22006	44334-44335	-RRB-	denotes	)
T22007	44336-44357	NN	denotes	coimmunoprecipitation
T22008	44358-44360	IN	denotes	of
T22009	44361-44364	NNP	denotes	Tat
T22010	44365-44369	IN	denotes	with
T22011	44370-44375	NNP	denotes	IκB-α
T22012	44376-44379	CC	denotes	and
T22013	44380-44383	CD	denotes	p65
T22014	44383-44384	:	denotes	;
T22015	44385-44386	-LRB-	denotes	(
T22016	44386-44389	FW	denotes	iii
T22017	44389-44390	-RRB-	denotes	)
T22018	44391-44400	NN	denotes	induction
T22019	44401-44403	IN	denotes	of
T22020	44404-44410	JJ	denotes	MIP-1α
T22021	44411-44421	NN	denotes	expression
T22022	44422-44431	JJ	denotes	dependent
T22023	44432-44434	IN	denotes	on
T22024	44435-44438	NNP	denotes	Tat
T22025	44439-44442	CC	denotes	and
T22026	44443-44446	CD	denotes	p65
T22027	44446-44447	:	denotes	;
T22028	44448-44449	-LRB-	denotes	(
T22029	44449-44451	NN	denotes	iv
T22030	44451-44452	-RRB-	denotes	)
T22031	44453-44456	NN	denotes	Tat
T22032	44457-44466	NN	denotes	occupancy
T22033	44467-44469	IN	denotes	of
T22034	44470-44473	DT	denotes	the
T22035	44474-44480	NNP	denotes	MIP-1α
T22036	44481-44486	NNP	denotes	NF-κB
T22037	44487-44495	NN	denotes	enhancer
T22038	44496-44506	VBN	denotes	associated
T22039	44507-44511	IN	denotes	with
T22040	44512-44521	VBN	denotes	increased
T22041	44522-44533	NN	denotes	recruitment
T22042	44534-44536	IN	denotes	of
T22043	44537-44540	NNS	denotes	p65
T22044	44540-44541	.	denotes	.
T22045	44542-44546	DT	denotes	This
T22046	44547-44552	NN	denotes	study
T22047	44553-44561	VBZ	denotes	supports
T22048	44562-44565	DT	denotes	the
T22049	44566-44582	JJ	denotes	pro-inflammatory
T22050	44583-44589	NN	denotes	action
T22051	44590-44592	IN	denotes	of
T22052	44593-44596	NNP	denotes	Tat
T22053	44597-44604	IN	denotes	through
T22054	44605-44613	JJ	denotes	physical
T22055	44614-44625	NN	denotes	interaction
T22056	44626-44630	IN	denotes	with
T22057	44631-44634	CD	denotes	p65
T22058	44635-44638	CC	denotes	and
T22059	44639-44644	NNP	denotes	IκB-α
T22060	44644-44645	.	denotes	.
T22061	44646-44653	JJ	denotes	Several
T22062	44654-44666	JJ	denotes	inflammatory
T22063	44667-44676	NNS	denotes	cytokines
T22064	44677-44682	IN	denotes	under
T22065	44683-44686	DT	denotes	the
T22066	44687-44702	JJ	denotes	transcriptional
T22067	44703-44710	NN	denotes	control
T22068	44711-44713	IN	denotes	of
T22069	44714-44719	NNP	denotes	NF-κB
T22070	44720-44723	VBP	denotes	are
T22071	44724-44739	JJ	denotes	hyper-expressed
T22072	44740-44742	IN	denotes	in
T22073	44743-44748	JJ	denotes	HIV-1
T22074	44749-44757	JJ	denotes	infected
T22075	44758-44769	NNS	denotes	individuals
T22076	44769-44770	,	denotes	,
T22077	44771-44780	VBG	denotes	including
T22078	44781-44785	NNP	denotes	IL-6
T22079	44786-44787	-LRB-	denotes	(
T22080	44787-44792	CD	denotes	66,67
T22081	44792-44793	-RRB-	denotes	)
T22082	44793-44794	,	denotes	,
T22083	44795-44800	NNP	denotes	TNF-α
T22084	44801-44802	-LRB-	denotes	(
T22085	44802-44804	CD	denotes	68
T22086	44804-44805	-RRB-	denotes	)
T22087	44805-44806	,	denotes	,
T22088	44807-44810	CC	denotes	and
T22089	44811-44817	NNP	denotes	MIP-1α
T22090	44818-44819	-LRB-	denotes	(
T22091	44819-44821	CD	denotes	57
T22092	44822-44827	CD	denotes	62,69
T22093	44828-44830	CD	denotes	71
T22094	44830-44831	-RRB-	denotes	)
T22095	44831-44832	.	denotes	.
T22096	44833-44835	IN	denotes	In
T22097	44836-44839	DT	denotes	the
T22098	44840-44844	NN	denotes	case
T22099	44845-44847	IN	denotes	of
T22100	44848-44853	NNP	denotes	TNF-α
T22101	44854-44857	CC	denotes	and
T22102	44858-44862	NNP	denotes	IL-6
T22103	44862-44863	,	denotes	,
T22104	44864-44867	NNP	denotes	Tat
T22105	44868-44877	VBD	denotes	activated
T22106	44878-44881	DT	denotes	the
T22107	44882-44890	NN	denotes	cytokine
T22108	44891-44901	NN	denotes	expression
T22109	44902-44904	IN	denotes	by
T22110	44905-44912	JJ	denotes	binding
T22111	44913-44915	TO	denotes	to
T22112	44916-44919	DT	denotes	the
T22113	44920-44928	JJ	denotes	TAR-like
T22114	44929-44933	NN	denotes	stem
T22115	44934-44938	NN	denotes	loop
T22116	44939-44941	IN	denotes	of
T22117	44942-44945	DT	denotes	the
T22118	44946-44947	CD	denotes	5
T22119	44947-44948	NN	denotes	′
T22120	44949-44959	NN	denotes	transcript
T22121	44959-44960	,	denotes	,
T22122	44961-44965	RB	denotes	thus
T22123	44966-44972	JJ	denotes	likely
T22124	44973-44982	VBG	denotes	promoting
T22125	44983-44986	DT	denotes	the
T22126	44987-45002	JJ	denotes	transcriptional
T22127	45003-45013	NN	denotes	elongation
T22128	45014-45015	-LRB-	denotes	(
T22129	45015-45023	CD	denotes	33,37,47
T22130	45023-45024	-RRB-	denotes	)
T22131	45024-45025	.	denotes	.
T22132	45026-45030	RB	denotes	Here
T22133	45030-45031	,	denotes	,
T22134	45032-45034	PRP	denotes	we
T22135	45035-45039	VBP	denotes	have
T22136	45040-45045	VBN	denotes	shown
T22137	45046-45050	IN	denotes	that
T22138	45051-45054	DT	denotes	the
T22139	45055-45068	JJ	denotes	Tat-dependent
T22140	45069-45084	JJ	denotes	transcriptional
T22141	45085-45095	NN	denotes	activation
T22142	45096-45098	IN	denotes	of
T22143	45099-45100	DT	denotes	a
T22144	45101-45107	NN	denotes	number
T22145	45108-45110	IN	denotes	of
T22146	45111-45127	JJ	denotes	pro-inflammatory
T22147	45128-45133	NNS	denotes	genes
T22148	45134-45142	VBD	denotes	required
T22149	45143-45146	DT	denotes	the
T22150	45147-45155	NN	denotes	assembly
T22151	45156-45158	IN	denotes	of
T22152	45159-45162	NNP	denotes	Tat
T22153	45163-45167	IN	denotes	with
T22154	45168-45171	CD	denotes	p65
T22155	45172-45174	IN	denotes	at
T22156	45175-45178	DT	denotes	the
T22157	45179-45184	NNP	denotes	NF-κB
T22158	45185-45193	NN	denotes	enhancer
T22159	45194-45202	RB	denotes	together
T22160	45203-45207	IN	denotes	with
T22161	45208-45211	DT	denotes	the
T22162	45212-45224	NN	denotes	displacement
T22163	45225-45227	IN	denotes	of
T22164	45228-45233	NNP	denotes	IκB-α
T22165	45234-45235	-LRB-	denotes	(
T22166	45235-45241	NNP	denotes	Figure
T22167	45242-45243	CD	denotes	5
T22168	45243-45244	-RRB-	denotes	)
T22169	45244-45245	.	denotes	.
T22170	45246-45250	RB	denotes	Thus
T22171	45250-45251	,	denotes	,
T22172	45252-45254	IN	denotes	in
T22173	45255-45258	DT	denotes	the
T22174	45259-45262	NN	denotes	set
T22175	45263-45265	IN	denotes	of
T22176	45266-45274	JJ	denotes	distinct
T22177	45275-45291	JJ	denotes	NF-κB-responsive
T22178	45292-45297	NNS	denotes	genes
T22179	45297-45298	,	denotes	,
T22180	45299-45302	NNP	denotes	Tat
T22181	45303-45308	VBD	denotes	acted
T22182	45309-45311	IN	denotes	as
T22183	45312-45313	DT	denotes	a
T22184	45314-45323	NN	denotes	component
T22185	45324-45326	IN	denotes	of
T22186	45327-45330	DT	denotes	the
T22187	45331-45346	JJ	denotes	transcriptional
T22188	45347-45357	NN	denotes	initiation
T22189	45358-45365	NN	denotes	complex
T22190	45365-45366	,	denotes	,
T22191	45367-45372	WDT	denotes	which
T22192	45373-45375	VBZ	denotes	is
T22193	45376-45386	JJ	denotes	consistent
T22194	45387-45391	IN	denotes	with
T22195	45392-45400	JJ	denotes	previous
T22196	45401-45408	NNS	denotes	reports
T22197	45409-45411	IN	denotes	on
T22198	45412-45415	NNP	denotes	Tat
T22199	45416-45425	VBG	denotes	promoting
T22200	45426-45429	DT	denotes	the
T22201	45430-45443	NN	denotes	transcription
T22202	45444-45447	IN	denotes	via
T22203	45448-45451	NNP	denotes	DNA
T22204	45452-45458	NNS	denotes	motifs
T22205	45459-45460	-LRB-	denotes	(
T22206	45460-45465	CD	denotes	72,73
T22207	45465-45466	-RRB-	denotes	)
T22208	45466-45467	.	denotes	.
T22209	45468-45482	NN	denotes	Overproduction
T22210	45483-45485	IN	denotes	of
T22211	45486-45502	JJ	denotes	pro-inflammatory
T22212	45503-45512	NNS	denotes	cytokines
T22213	45513-45515	VBZ	denotes	is
T22214	45516-45526	VBN	denotes	considered
T22215	45527-45528	DT	denotes	a
T22216	45529-45534	JJ	denotes	major
T22217	45535-45544	NN	denotes	mechanism
T22218	45545-45547	IN	denotes	of
T22219	45548-45554	JJ	denotes	immune
T22220	45555-45567	NN	denotes	deregulation
T22221	45568-45569	-LRB-	denotes	(
T22222	45569-45571	CD	denotes	26
T22223	45571-45572	-RRB-	denotes	)
T22224	45573-45576	CC	denotes	and
T22225	45577-45583	NN	denotes	neuron
T22226	45584-45595	NN	denotes	dysfunction
T22227	45596-45598	IN	denotes	in
T22228	45599-45603	NNP	denotes	AIDS
T22229	45604-45605	-LRB-	denotes	(
T22230	45605-45610	CD	denotes	74,75
T22231	45610-45611	-RRB-	denotes	)
T22232	45611-45612	.	denotes	.
T22233	45613-45615	IN	denotes	In
T22234	45616-45620	DT	denotes	this
T22235	45621-45629	NN	denotes	scenario
T22236	45629-45630	,	denotes	,
T22237	45631-45641	NN	denotes	inhibition
T22238	45642-45644	IN	denotes	of
T22239	45645-45650	JJ	denotes	NF-κB
T22240	45651-45659	NN	denotes	activity
T22241	45660-45665	MD	denotes	could
T22242	45666-45670	VB	denotes	lead
T22243	45671-45673	TO	denotes	to
T22244	45674-45675	DT	denotes	a
T22245	45676-45687	JJ	denotes	therapeutic
T22246	45688-45696	NN	denotes	strategy
T22247	45697-45700	IN	denotes	for
T22248	45701-45714	VBG	denotes	counteracting
T22249	45715-45718	DT	denotes	the
T22250	45719-45727	JJ	denotes	abnormal
T22251	45728-45738	NN	denotes	production
T22252	45739-45741	IN	denotes	of
T22253	45742-45758	JJ	denotes	pro-inflammatory
T22254	45759-45768	NNS	denotes	cytokines
T22255	45769-45772	CC	denotes	and
T22256	45773-45783	NNS	denotes	chemokines
T22257	45784-45786	IN	denotes	in
T22258	45787-45792	JJ	denotes	HIV-1
T22259	45793-45802	NN	denotes	infection
T22260	45802-45803	.	denotes	.
T22261	45804-45809	JJ	denotes	NF-κB
T22262	45810-45820	NNS	denotes	inhibitors
T22263	45820-45821	,	denotes	,
T22264	45822-45826	JJ	denotes	such
T22265	45827-45829	IN	denotes	as
T22266	45830-45843	NNS	denotes	anti-oxidants
T22267	45843-45844	,	denotes	,
T22268	45845-45855	JJ	denotes	proteasome
T22269	45856-45859	CC	denotes	and
T22270	45860-45863	NNP	denotes	IKK
T22271	45864-45874	NNS	denotes	inhibitors
T22272	45874-45875	,	denotes	,
T22273	45876-45889	RB	denotes	significantly
T22274	45890-45897	VBD	denotes	reduced
T22275	45898-45901	DT	denotes	the
T22276	45902-45907	NNP	denotes	HIV-1
T22277	45908-45919	NN	denotes	replication
T22278	45920-45923	CC	denotes	and
T22279	45924-45927	DT	denotes	the
T22280	45928-45938	VBN	denotes	associated
T22281	45939-45951	JJ	denotes	inflammatory
T22282	45952-45960	NN	denotes	response
T22283	45961-45962	-LRB-	denotes	(
T22284	45962-45967	CD	denotes	76,77
T22285	45967-45968	-RRB-	denotes	)
T22286	45968-45969	:	denotes	;
T22287	45970-45977	RB	denotes	however
T22288	45977-45978	,	denotes	,
T22289	45979-45984	DT	denotes	these
T22290	45985-45990	JJ	denotes	NF-κB
T22291	45991-46001	NNS	denotes	inhibitors
T22292	46002-46007	VBD	denotes	acted
T22293	46008-46024	RB	denotes	indiscriminately
T22294	46025-46027	IN	denotes	in
T22295	46028-46032	DT	denotes	both
T22296	46033-46047	JJ	denotes	HIV-1-infected
T22297	46048-46051	CC	denotes	and
T22298	46052-46062	JJ	denotes	uninfected
T22299	46063-46068	NNS	denotes	cells
T22300	46068-46069	.	denotes	.
T22301	46070-46073	DT	denotes	The
T22302	46074-46082	NN	denotes	evidence
T22303	46083-46087	IN	denotes	that
T22304	46088-46091	NNP	denotes	Tat
T22305	46092-46101	VBZ	denotes	activates
T22306	46102-46107	NN	denotes	NF-κB
T22307	46108-46115	IN	denotes	through
T22308	46116-46122	JJ	denotes	direct
T22309	46123-46134	NN	denotes	interaction
T22310	46135-46139	IN	denotes	with
T22311	46140-46145	NNP	denotes	IκB-α
T22312	46146-46149	CC	denotes	and
T22313	46150-46153	NNS	denotes	p65
T22314	46154-46157	MD	denotes	may
T22315	46158-46162	VB	denotes	lead
T22316	46163-46165	TO	denotes	to
T22317	46166-46174	JJ	denotes	specific
T22318	46175-46185	NNS	denotes	inhibitors
T22319	46186-46188	TO	denotes	to
T22320	46189-46199	VB	denotes	counteract
T22321	46200-46203	DT	denotes	the
T22322	46204-46207	NNP	denotes	Tat
T22323	46208-46224	JJ	denotes	pro-inflammatory
T22324	46225-46231	NN	denotes	action
T22325	46231-46232	.	denotes	.
T23945	16062-16065	NNP	denotes	Tat
T23946	16066-16077	VBZ	denotes	counteracts
T23947	16078-16081	DT	denotes	the
T23948	16082-16097	JJ	denotes	post-activation
T23949	16098-16102	NN	denotes	turn
T23950	16103-16106	IN	denotes	off
T23951	16107-16109	IN	denotes	of
T23952	16110-16115	NN	denotes	NF-κB
T23953	16116-16118	IN	denotes	in
T23954	16119-16125	JJ	denotes	single
T23955	16126-16131	NN	denotes	round
T23956	16132-16147	NN	denotes	HIV-1-infection
T23957	16147-16148	.	denotes	.
T23958	16149-16155	NNP	denotes	Jurkat
T23959	16156-16161	NNS	denotes	cells
T23960	16162-16163	-LRB-	denotes	(
T23961	16163-16164	CD	denotes	5
T23962	16165-16166	RB	denotes	×
T23963	16167-16170	CD	denotes	107
T23964	16170-16171	-RRB-	denotes	)
T23965	16172-16176	VBD	denotes	were
T23966	16177-16188	VBN	denotes	transfected
T23967	16189-16193	IN	denotes	with
T23968	16194-16199	NNP	denotes	siRNA
T23969	16200-16203	NNP	denotes	Tat
T23970	16204-16206	CC	denotes	or
T23971	16207-16212	NNP	denotes	siRNA
T23972	16213-16220	NN	denotes	control
T23973	16221-16222	-LRB-	denotes	(
T23974	16222-16223	CD	denotes	2
T23975	16224-16228	NN	denotes	nmol
T23976	16228-16229	-RRB-	denotes	)
T23977	16229-16230	,	denotes	,
T23978	16231-16233	CC	denotes	or
T23979	16234-16238	VBD	denotes	left
T23980	16239-16252	JJ	denotes	untransfected
T23981	16252-16253	:	denotes	;
T23982	16254-16256	CD	denotes	24
T23983	16257-16258	NN	denotes	h
T23984	16259-16264	RB	denotes	later
T23985	16264-16265	,	denotes	,
T23986	16266-16271	NNS	denotes	cells
T23987	16272-16276	VBD	denotes	were
T23988	16277-16285	VBN	denotes	infected
T23989	16286-16290	IN	denotes	with
T23990	16291-16307	JJ	denotes	HXB2-pseudotyped
T23991	16308-16313	NN	denotes	NL4-3
T23992	16313-16314	.	denotes	.
T23993	16314-16319	NNP	denotes	Luc.R
T23994	16319-16320	NNP	denotes	−
T23995	16320-16321	NNP	denotes	E
T23996	16321-16322	VBD	denotes	−
T23997	16323-16330	NNS	denotes	virions
T23998	16331-16332	-LRB-	denotes	(
T23999	16332-16335	CD	denotes	500
T24000	16336-16338	NN	denotes	ng
T24001	16339-16341	IN	denotes	of
T24002	16342-16345	CD	denotes	p24
T24003	16345-16346	-RRB-	denotes	)
T24004	16347-16350	CC	denotes	and
T24005	16351-16360	VBD	denotes	harvested
T24006	16361-16363	IN	denotes	at
T24007	16364-16367	DT	denotes	the
T24008	16368-16377	JJ	denotes	indicated
T24009	16378-16382	NN	denotes	time
T24010	16382-16383	.	denotes	.
T24011	16384-16385	-LRB-	denotes	(
T24012	16385-16386	NNP	denotes	A
T24013	16386-16387	-RRB-	denotes	)
T24014	16388-16391	NNP	denotes	Tat
T24015	16392-16402	NN	denotes	expression
T24016	16403-16406	VBD	denotes	was
T24017	16407-16415	VBN	denotes	measured
T24018	16416-16418	IN	denotes	in
T24019	16419-16424	JJ	denotes	total
T24020	16425-16428	NNP	denotes	RNA
T24021	16429-16431	IN	denotes	by
T24022	16432-16441	JJ	denotes	real-time
T24023	16442-16445	NNP	denotes	PCR
T24024	16445-16446	,	denotes	,
T24025	16447-16452	IN	denotes	while
T24026	16453-16456	DT	denotes	the
T24027	16457-16467	NN	denotes	luciferase
T24028	16468-16476	NN	denotes	activity
T24029	16477-16480	VBD	denotes	was
T24030	16481-16489	VBN	denotes	measured
T24031	16490-16492	IN	denotes	in
T24032	16493-16498	JJ	denotes	whole
T24033	16499-16503	NN	denotes	cell
T24034	16504-16512	NNS	denotes	extracts
T24035	16512-16513	.	denotes	.
T24036	16514-16515	-LRB-	denotes	(
T24037	16515-16516	NNP	denotes	B
T24038	16516-16517	-RRB-	denotes	)
T24039	16518-16525	NNP	denotes	Nuclear
T24040	16526-16534	NNS	denotes	extracts
T24041	16535-16536	-LRB-	denotes	(
T24042	16536-16538	CD	denotes	10
T24043	16539-16541	NN	denotes	µg
T24044	16541-16542	-RRB-	denotes	)
T24045	16543-16547	VBD	denotes	were
T24046	16548-16556	VBN	denotes	analysed
T24047	16557-16560	IN	denotes	for
T24048	16561-16564	DT	denotes	the
T24049	16565-16568	NNS	denotes	p65
T24050	16569-16572	CC	denotes	and
T24051	16573-16576	JJ	denotes	p50
T24052	16577-16584	JJ	denotes	binding
T24053	16585-16587	TO	denotes	to
T24054	16588-16591	DT	denotes	the
T24055	16592-16597	NNP	denotes	NF-κB
T24056	16598-16613	JJ	denotes	double-stranded
T24057	16614-16629	NN	denotes	oligonucleotide
T24058	16630-16635	VBG	denotes	using
T24059	16636-16639	DT	denotes	the
T24060	16640-16645	NNP	denotes	NF-κB
T24061	16646-16659	NNP	denotes	Transcription
T24062	16660-16666	NNP	denotes	Factor
T24063	16667-16672	NNP	denotes	ELISA
T24064	16673-16678	NNP	denotes	Assay
T24065	16679-16682	NN	denotes	kit
T24066	16683-16684	-LRB-	denotes	(
T24067	16684-16690	NNP	denotes	Cayman
T24068	16690-16691	-RRB-	denotes	)
T24069	16691-16692	.	denotes	.
T24070	16693-16694	-LRB-	denotes	(
T24071	16694-16695	$	denotes	C
T24072	16695-16696	-RRB-	denotes	)
T24073	16697-16700	DT	denotes	The
T24074	16701-16711	NN	denotes	expression
T24075	16712-16714	IN	denotes	of
T24076	16715-16718	CD	denotes	p65
T24077	16719-16722	CC	denotes	and
T24078	16723-16728	NNP	denotes	IκB-α
T24079	16729-16732	VBD	denotes	was
T24080	16733-16741	VBN	denotes	analysed
T24081	16742-16744	IN	denotes	by
T24082	16745-16747	CD	denotes	12
T24083	16747-16748	NN	denotes	%
T24084	16749-16752	NNP	denotes	SDS
T24085	16753-16757	NNP	denotes	PAGE
T24086	16758-16761	CC	denotes	and
T24087	16762-16769	JJ	denotes	western
T24088	16770-16778	VBG	denotes	blotting
T24089	16779-16781	IN	denotes	of
T24090	16782-16789	JJ	denotes	nuclear
T24091	16790-16792	CC	denotes	or
T24092	16793-16802	JJ	denotes	cytosolic
T24093	16803-16811	NNS	denotes	extracts
T24094	16812-16813	-LRB-	denotes	(
T24095	16813-16815	CD	denotes	20
T24096	16816-16818	NN	denotes	µg
T24097	16818-16819	-RRB-	denotes	)
T24098	16820-16825	VBG	denotes	using
T24099	16826-16834	JJ	denotes	anti-p65
T24100	16835-16838	CC	denotes	and
T24101	16839-16849	JJ	denotes	anti-IκB-α
T24102	16850-16860	NNS	denotes	antibodies
T24103	16860-16861	.	denotes	.
T24104	16862-16869	NNP	denotes	Histone
T24105	16870-16872	NNP	denotes	H1
T24114	16930-16932	IN	denotes	as
T24115	16933-16940	NNS	denotes	markers
T24116	16941-16943	IN	denotes	of
T24117	16944-16951	JJ	denotes	nuclear
T24118	16952-16955	CC	denotes	and
T24119	16956-16965	JJ	denotes	cytosolic
T24120	16966-16974	NNS	denotes	extracts
T24121	16974-16975	,	denotes	,
T24122	16976-16988	RB	denotes	respectively
T24123	16988-16989	.	denotes	.
T24124	16990-17002	NNP	denotes	Densitometry
T24125	17003-17009	NNS	denotes	values
T24126	17010-17011	-LRB-	denotes	(
T24127	17011-17012	NNP	denotes	D
T24128	17012-17013	-RRB-	denotes	)
T24129	17014-17016	IN	denotes	of
T24130	17017-17020	DT	denotes	the
T24131	17021-17026	NNS	denotes	bands
T24132	17027-17031	VBD	denotes	were
T24133	17032-17041	VBN	denotes	expressed
T24134	17042-17044	IN	denotes	as
T24135	17045-17049	VBP	denotes	fold
T24136	17050-17058	NN	denotes	increase
T24137	17059-17064	IN	denotes	above
T24138	17065-17068	DT	denotes	the
T24139	17069-17076	NN	denotes	control
T24140	17077-17078	-LRB-	denotes	(
T24141	17078-17082	JJ	denotes	mock
T24142	17082-17083	-RRB-	denotes	)
T24143	17083-17084	.	denotes	.
T24144	17085-17086	-LRB-	denotes	(
T24145	17086-17087	NNP	denotes	D
T24146	17087-17088	-RRB-	denotes	)
T24147	17089-17092	NNP	denotes	IKK
T24148	17093-17101	NN	denotes	activity
T24149	17102-17105	VBD	denotes	was
T24150	17106-17114	VBN	denotes	measured
T24151	17115-17117	IN	denotes	in
T24152	17118-17127	JJ	denotes	cytosolic
T24153	17128-17136	NNS	denotes	extracts
T24154	17137-17138	-LRB-	denotes	(
T24155	17138-17141	CD	denotes	100
T24156	17142-17144	NN	denotes	µg
T24157	17144-17145	-RRB-	denotes	)
T24158	17146-17148	IN	denotes	by
T24159	17149-17154	VBG	denotes	using
T24160	17155-17161	NNP	denotes	HTScan
T24161	17162-17165	NNP	denotes	IKK
T24162	17166-17172	NNP	denotes	Kinase
T24163	17173-17178	NNP	denotes	Assay
T24164	17179-17180	-LRB-	denotes	(
T24165	17180-17184	NNP	denotes	Cell
T24166	17185-17194	NNP	denotes	Signaling
T24167	17195-17205	NNP	denotes	Technology
T24168	17205-17206	-RRB-	denotes	)
T24169	17206-17207	.	denotes	.
T24170	17208-17214	NNS	denotes	Values
T24171	17215-17216	-LRB-	denotes	(
T24172	17216-17220	JJ	denotes	mean
T24173	17221-17222	NN	denotes	±
T24174	17223-17225	NNP	denotes	SE
T24175	17225-17226	,	denotes	,
T24176	17227-17228	JJ	denotes	n
T24177	17229-17230	SYM	denotes	=
T24178	17231-17232	LS	denotes	3
T24179	17232-17233	-RRB-	denotes	)
T24180	17234-17237	VBP	denotes	are
T24181	17238-17243	VBN	denotes	shown
T24182	17243-17244	.	denotes	.
T24672	20207-20210	NNP	denotes	Tat
T24673	20211-20219	VBZ	denotes	enhances
T24674	20220-20223	DT	denotes	the
T24675	20224-20229	JJ	denotes	NF-κB
T24676	20230-20238	NN	denotes	activity
T24677	20239-20248	VBG	denotes	following
T24678	20249-20252	NNP	denotes	PMA
T24679	20253-20264	NN	denotes	stimulation
T24680	20265-20267	IN	denotes	by
T24681	20268-20279	VBG	denotes	associating
T24682	20280-20284	IN	denotes	with
T24683	20285-20290	NNP	denotes	IκB-α
T24684	20291-20294	CC	denotes	and
T24685	20295-20308	VBG	denotes	counteracting
T24686	20309-20312	DT	denotes	the
T24687	20313-20318	JJ	denotes	NF-κB
T24688	20319-20329	NN	denotes	repression
T24689	20329-20330	.	denotes	.
T24690	20331-20335	NNP	denotes	HeLa
T24691	20336-20341	NNS	denotes	cells
T24692	20342-20343	-LRB-	denotes	(
T24693	20343-20344	CD	denotes	5
T24694	20345-20346	CD	denotes	×
T24695	20347-20350	CD	denotes	106
T24696	20350-20351	-RRB-	denotes	)
T24697	20352-20356	VBD	denotes	were
T24698	20357-20368	VBN	denotes	transfected
T24699	20369-20373	IN	denotes	with
T24700	20374-20385	NN	denotes	p3xFLAG-Tat
T24701	20386-20388	CC	denotes	or
T24702	20389-20396	JJ	denotes	p3xFLAG
T24703	20397-20402	JJ	denotes	empty
T24704	20403-20409	NN	denotes	vector
T24705	20410-20411	-LRB-	denotes	(
T24706	20411-20412	CD	denotes	5
T24707	20413-20415	NN	denotes	µg
T24708	20415-20416	-RRB-	denotes	)
T24709	20416-20417	,	denotes	,
T24710	20418-20421	CC	denotes	and
T24711	20422-20424	CD	denotes	48
T24712	20425-20426	NN	denotes	h
T24713	20427-20432	RB	denotes	later
T24714	20433-20437	VBD	denotes	were
T24715	20438-20448	VBN	denotes	stimulated
T24716	20449-20453	IN	denotes	with
T24717	20454-20457	NNP	denotes	PMA
T24718	20458-20459	-LRB-	denotes	(
T24719	20459-20460	CD	denotes	5
T24720	20461-20464	NN	denotes	min
T24721	20464-20465	,	denotes	,
T24722	20466-20468	CD	denotes	20
T24723	20469-20474	NN	denotes	ng/ml
T24724	20474-20475	-RRB-	denotes	)
T24725	20476-20478	CC	denotes	or
T24726	20479-20483	VBD	denotes	left
T24727	20484-20496	JJ	denotes	unstimulated
T24728	20496-20497	,	denotes	,
T24729	20498-20504	VBD	denotes	washed
T24730	20505-20510	RB	denotes	twice
T24731	20511-20515	IN	denotes	with
T24732	20516-20520	NNP	denotes	DMEM
T24733	20521-20524	CC	denotes	and
T24734	20525-20534	VBN	denotes	harvested
T24735	20535-20537	IN	denotes	at
T24736	20538-20541	DT	denotes	the
T24737	20542-20551	JJ	denotes	indicated
T24738	20552-20557	NNS	denotes	times
T24739	20557-20558	.	denotes	.
T24740	20559-20566	NNP	denotes	Nuclear
T24741	20567-20570	CC	denotes	and
T24742	20571-20580	JJ	denotes	cytosolic
T24743	20581-20589	NNS	denotes	extracts
T24744	20590-20594	VBD	denotes	were
T24745	20595-20603	VBN	denotes	prepared
T24746	20604-20607	IN	denotes	for
T24747	20608-20615	JJ	denotes	further
T24748	20616-20624	NN	denotes	analysis
T24749	20624-20625	.	denotes	.
T24750	20626-20627	-LRB-	denotes	(
T24751	20627-20628	NNP	denotes	A
T24752	20628-20629	-RRB-	denotes	)
T24753	20630-20637	NNP	denotes	Nuclear
T24754	20638-20646	NNS	denotes	extracts
T24755	20647-20651	VBD	denotes	were
T24756	20652-20660	VBN	denotes	analysed
T24757	20661-20664	IN	denotes	for
T24758	20665-20668	DT	denotes	the
T24759	20669-20672	NNS	denotes	p65
T24760	20672-20673	,	denotes	,
T24761	20674-20677	JJ	denotes	p50
T24762	20678-20681	CC	denotes	and
T24763	20682-20690	JJ	denotes	FLAG-Tat
T24764	20691-20698	JJ	denotes	binding
T24765	20699-20701	TO	denotes	to
T24766	20702-20705	DT	denotes	the
T24767	20706-20711	NNP	denotes	NF-κB
T24768	20712-20727	JJ	denotes	double-stranded
T24769	20728-20743	NN	denotes	oligonucleotide
T24770	20744-20749	VBG	denotes	using
T24771	20750-20753	DT	denotes	the
T24772	20754-20759	NNP	denotes	NF-κB
T24773	20760-20773	NNP	denotes	Transcription
T24774	20774-20780	NNP	denotes	Factor
T24775	20781-20786	NNP	denotes	ELISA
T24776	20787-20792	NN	denotes	assay
T24777	20793-20796	NN	denotes	kit
T24778	20797-20798	-LRB-	denotes	(
T24779	20798-20804	NNP	denotes	Cayman
T24780	20804-20805	-RRB-	denotes	)
T24781	20805-20806	.	denotes	.
T24782	20807-20808	-LRB-	denotes	(
T24783	20808-20809	NNP	denotes	B
T24784	20809-20810	-RRB-	denotes	)
T24785	20811-20818	NNP	denotes	Nuclear
T24786	20819-20822	CC	denotes	and
T24787	20823-20832	JJ	denotes	cytosolic
T24788	20833-20841	NNS	denotes	extracts
T24789	20842-20843	-LRB-	denotes	(
T24790	20843-20845	CD	denotes	20
T24791	20846-20848	NN	denotes	µg
T24792	20848-20849	-RRB-	denotes	)
T24793	20850-20854	VBD	denotes	were
T24794	20855-20864	VBN	denotes	separated
T24795	20865-20867	IN	denotes	by
T24796	20868-20870	CD	denotes	12
T24797	20870-20871	NN	denotes	%
T24798	20872-20875	NNP	denotes	SDS
T24799	20876-20880	NNP	denotes	PAGE
T24800	20881-20884	CC	denotes	and
T24801	20885-20893	VBN	denotes	analysed
T24802	20894-20896	IN	denotes	by
T24803	20897-20904	JJ	denotes	western
T24804	20905-20913	VBG	denotes	blotting
T24805	20914-20919	VBG	denotes	using
T24806	20920-20923	DT	denotes	the
T24807	20924-20932	JJ	denotes	anti-p65
T24808	20932-20933	,	denotes	,
T24809	20934-20943	JJ	denotes	anti-FLAG
T24810	20943-20944	,	denotes	,
T24811	20945-20957	JJ	denotes	anti-Histone
T24812	20958-20960	NN	denotes	H1
T24813	20960-20961	,	denotes	,
T24814	20962-20972	JJ	denotes	anti-IκB-α
T24815	20973-20976	CC	denotes	and
T24816	20977-20995	JJ	denotes	anti-Hexokinase-II
T24817	20996-21006	NNS	denotes	antibodies
T24818	21006-21007	.	denotes	.
T24819	21008-21020	NNP	denotes	Densitometry
T24820	21021-21027	NNS	denotes	values
T24821	21028-21029	-LRB-	denotes	(
T24822	21029-21030	NNP	denotes	D
T24823	21030-21031	-RRB-	denotes	)
T24824	21032-21034	IN	denotes	of
T24825	21035-21038	DT	denotes	the
T24826	21039-21044	NNS	denotes	bands
T24827	21045-21049	VBD	denotes	were
T24828	21050-21059	VBN	denotes	expressed
T24829	21060-21062	IN	denotes	as
T24830	21063-21067	VBP	denotes	fold
T24831	21068-21076	NN	denotes	increase
T24832	21077-21082	IN	denotes	above
T24833	21083-21086	DT	denotes	the
T24834	21087-21094	NN	denotes	control
T24835	21095-21096	-LRB-	denotes	(
T24836	21096-21100	NN	denotes	lane
T24837	21101-21102	CD	denotes	1
T24838	21102-21103	-RRB-	denotes	)
T24839	21103-21104	.	denotes	.
T24840	21105-21106	-LRB-	denotes	(
T24841	21106-21107	NNP	denotes	C
T24842	21107-21108	-RRB-	denotes	)
T24843	21109-21112	NNP	denotes	IKK
T24844	21113-21121	NN	denotes	activity
T24845	21122-21125	VBD	denotes	was
T24846	21126-21134	VBN	denotes	measured
T24847	21135-21137	IN	denotes	in
T24848	21138-21147	JJ	denotes	cytosolic
T24849	21148-21156	NNS	denotes	extracts
T24850	21157-21158	-LRB-	denotes	(
T24851	21158-21161	CD	denotes	100
T24852	21162-21164	NN	denotes	µg
T24853	21164-21165	-RRB-	denotes	)
T24854	21166-21168	IN	denotes	by
T24855	21169-21174	VBG	denotes	using
T24856	21175-21181	NNP	denotes	HTScan
T24857	21182-21185	NNP	denotes	IKK
T24858	21186-21192	NN	denotes	kinase
T24859	21193-21198	NN	denotes	assay
T24860	21199-21200	-LRB-	denotes	(
T24861	21200-21204	NNP	denotes	Cell
T24862	21205-21214	NNP	denotes	Signaling
T24863	21215-21225	NNP	denotes	Technology
T24864	21225-21226	-RRB-	denotes	)
T24865	21226-21227	.	denotes	.
T24866	21228-21229	-LRB-	denotes	(
T24867	21229-21230	NNP	denotes	D
T24868	21230-21231	-RRB-	denotes	)
T24869	21232-21236	NNP	denotes	HeLa
T24870	21237-21242	NNS	denotes	cells
T24871	21243-21244	-LRB-	denotes	(
T24872	21244-21245	CD	denotes	5
T24873	21246-21247	CD	denotes	×
T24874	21248-21251	CD	denotes	106
T24875	21251-21252	-RRB-	denotes	)
T24876	21253-21257	VBD	denotes	were
T24877	21258-21269	VBN	denotes	transfected
T24878	21270-21274	IN	denotes	with
T24879	21275-21277	CC	denotes	or
T24880	21278-21285	IN	denotes	without
T24881	21286-21297	NN	denotes	p3xFLAG-Tat
T24882	21298-21299	-LRB-	denotes	(
T24883	21299-21300	CD	denotes	5
T24884	21301-21303	NN	denotes	µg
T24885	21303-21304	-RRB-	denotes	)
T24886	21304-21305	:	denotes	;
T24887	21306-21308	CD	denotes	48
T24888	21309-21310	NN	denotes	h
T24889	21311-21316	RB	denotes	later
T24890	21316-21317	,	denotes	,
T24891	21318-21323	NNS	denotes	cells
T24892	21324-21328	VBD	denotes	were
T24893	21329-21339	VBN	denotes	stimulated
T24894	21340-21344	IN	denotes	with
T24895	21345-21348	NNP	denotes	PMA
T24896	21349-21350	-LRB-	denotes	(
T24897	21350-21351	CD	denotes	5
T24898	21352-21355	NN	denotes	min
T24899	21355-21356	,	denotes	,
T24900	21357-21359	CD	denotes	20
T24901	21360-21365	NN	denotes	ng/ml
T24902	21365-21366	-RRB-	denotes	)
T24903	21367-21369	CC	denotes	or
T24904	21370-21374	VBD	denotes	left
T24905	21375-21387	JJ	denotes	unstimulated
T24906	21387-21388	,	denotes	,
T24907	21389-21395	VBD	denotes	washed
T24908	21396-21401	RB	denotes	twice
T24909	21402-21406	IN	denotes	with
T24910	21407-21411	NNP	denotes	DMEM
T24911	21411-21412	,	denotes	,
T24912	21413-21416	CC	denotes	and
T24913	21417-21426	VBN	denotes	harvested
T24914	21427-21432	IN	denotes	after
T24915	21433-21436	CD	denotes	240
T24916	21437-21440	NN	denotes	min
T24917	21440-21441	.	denotes	.
T24918	21442-21447	JJ	denotes	Whole
T24919	21448-21452	NN	denotes	cell
T24920	21453-21461	NNS	denotes	extracts
T24921	21462-21463	-LRB-	denotes	(
T24922	21463-21464	CD	denotes	1
T24923	21465-21467	NN	denotes	mg
T24924	21467-21468	-RRB-	denotes	)
T24925	21469-21473	VBD	denotes	were
T24926	21474-21492	VBN	denotes	immunoprecipitated
T24927	21493-21497	IN	denotes	with
T24928	21498-21505	NN	denotes	protein
T24929	21506-21525	JJ	denotes	G-Sepharose-coupled
T24930	21526-21536	JJ	denotes	anti-IκB-α
T24931	21537-21545	NN	denotes	antibody
T24932	21545-21546	.	denotes	.
T24933	21547-21562	NNS	denotes	Immunocomplexes
T24934	21563-21567	VBD	denotes	were
T24935	21568-21577	VBN	denotes	separated
T24936	21578-21580	IN	denotes	by
T24937	21581-21583	CD	denotes	12
T24938	21583-21584	NN	denotes	%
T24939	21585-21588	NNP	denotes	SDS
T24940	21589-21593	NNP	denotes	PAGE
T24941	21594-21597	CC	denotes	and
T24942	21598-21606	VBN	denotes	analysed
T24943	21607-21609	IN	denotes	by
T24944	21610-21617	JJ	denotes	western
T24945	21618-21626	VBG	denotes	blotting
T24946	21627-21631	IN	denotes	with
T24947	21632-21640	JJ	denotes	anti-p65
T24948	21640-21641	,	denotes	,
T24949	21642-21651	JJ	denotes	anti-FLAG
T24950	21652-21655	CC	denotes	and
T24951	21656-21666	JJ	denotes	anti-IκB-α
T24952	21667-21677	NNS	denotes	antibodies
T24953	21677-21678	.	denotes	.
T24954	21679-21691	NNP	denotes	Densitometry
T24955	21692-21698	NNS	denotes	values
T24956	21699-21700	-LRB-	denotes	(
T24957	21700-21701	NNP	denotes	D
T24958	21701-21702	-RRB-	denotes	)
T24959	21703-21705	IN	denotes	of
T24960	21706-21709	DT	denotes	the
T24961	21710-21715	NNS	denotes	bands
T24962	21716-21720	VBD	denotes	were
T24963	21721-21730	VBN	denotes	expressed
T24964	21731-21733	IN	denotes	as
T24965	21734-21738	VBP	denotes	fold
T24966	21739-21747	NN	denotes	increase
T24967	21748-21753	IN	denotes	above
T24968	21754-21757	DT	denotes	the
T24969	21758-21765	NN	denotes	control
T24970	21766-21767	-LRB-	denotes	(
T24971	21767-21771	NN	denotes	lane
T24972	21772-21773	CD	denotes	1
T24973	21773-21774	-RRB-	denotes	)
T24974	21774-21775	.	denotes	.
T24975	21776-21782	NNS	denotes	Values
T24976	21783-21784	-LRB-	denotes	(
T24977	21784-21788	JJ	denotes	mean
T24978	21789-21790	NN	denotes	±
T24979	21791-21793	NNP	denotes	SE
T24980	21793-21794	,	denotes	,
T24981	21795-21796	JJ	denotes	n
T24982	21797-21798	SYM	denotes	=
T24983	21799-21800	LS	denotes	3
T24984	21800-21801	-RRB-	denotes	)
T24985	21802-21805	VBP	denotes	are
T24986	21806-21811	VBN	denotes	shown
T24987	21811-21812	.	denotes	.
T25677	23400-23403	NNP	denotes	Tat
T25678	23404-23412	VBZ	denotes	relieves
T25679	23413-23416	CD	denotes	p65
T25680	23417-23421	IN	denotes	from
T25681	23422-23425	DT	denotes	the
T25682	23426-23431	JJ	denotes	IκB-α
T25683	23432-23442	NN	denotes	inhibition
T25684	23442-23443	.	denotes	.
T25685	23444-23445	-LRB-	denotes	(
T25686	23445-23446	NN	denotes	A
T25687	23446-23447	-RRB-	denotes	)
T25688	23448-23457	JJ	denotes	Schematic
T25689	23458-23472	NN	denotes	representation
T25690	23473-23475	IN	denotes	of
T25691	23476-23485	JJ	denotes	wild-type
T25692	23486-23489	CC	denotes	and
T25693	23490-23496	JJ	denotes	mutant
T25694	23497-23500	NNP	denotes	Tat
T25695	23501-23509	NNS	denotes	proteins
T25696	23509-23510	.	denotes	.
T25697	23511-23512	-LRB-	denotes	(
T25698	23512-23513	NNP	denotes	B
T25699	23513-23514	-RRB-	denotes	)
T25700	23515-23523	NNP	denotes	HA-IκB-α
T25701	23524-23525	-LRB-	denotes	(
T25702	23525-23526	CD	denotes	5
T25703	23527-23529	NN	denotes	µl
T25704	23529-23530	-RRB-	denotes	)
T25705	23531-23534	VBD	denotes	was
T25706	23535-23544	VBN	denotes	incubated
T25707	23545-23547	IN	denotes	in
T25708	23548-23556	NN	denotes	presence
T25709	23557-23559	CC	denotes	or
T25710	23560-23567	NN	denotes	absence
T25711	23568-23570	IN	denotes	of
T25712	23571-23579	NNP	denotes	FLAG-Tat
T25713	23579-23580	,	denotes	,
T25714	23581-23589	NNP	denotes	FLAG-Tat
T25715	23590-23591	NNP	denotes	T
T25716	23591-23592	,	denotes	,
T25717	23792-23793	:	denotes	;
T25718	23592-23593	NNP	denotes	N
T25719	23593-23594	-LRB-	denotes	(
T25720	23594-23599	CD	denotes	23,24
T25721	23599-23600	-RRB-	denotes	)
T25722	23600-23601	DT	denotes	A
T25723	23601-23602	,	denotes	,
T25724	23603-23611	JJ	denotes	FLAG-Tat
T25725	23612-23613	NNP	denotes	K
T25726	23613-23614	-LRB-	denotes	(
T25727	23614-23619	CD	denotes	50,51
T25728	23619-23620	-RRB-	denotes	)
T25729	23620-23621	DT	denotes	A
T25730	23621-23622	,	denotes	,
T25731	23623-23631	JJ	denotes	FLAG-Tat
T25732	23632-23633	NN	denotes	R
T25733	23633-23634	-LRB-	denotes	(
T25734	23634-23636	CD	denotes	49
T25735	23637-23639	CD	denotes	57
T25736	23639-23640	-RRB-	denotes	)
T25737	23640-23641	DT	denotes	A
T25738	23642-23644	CC	denotes	or
T25739	23645-23653	JJ	denotes	FLAG-Tat
T25740	23654-23655	NNP	denotes	C
T25741	23655-23656	-LRB-	denotes	(
T25742	23656-23664	CD	denotes	22,25,27
T25743	23664-23665	-RRB-	denotes	)
T25744	23665-23666	DT	denotes	A
T25745	23667-23668	-LRB-	denotes	(
T25746	23668-23670	CD	denotes	10
T25747	23671-23673	NN	denotes	µl
T25748	23673-23674	-RRB-	denotes	)
T25749	23675-23680	VBG	denotes	using
T25750	23681-23683	IN	denotes	in
T25751	23684-23689	NN	denotes	vitro
T25752	23690-23700	VBN	denotes	translated
T25753	23701-23709	NNS	denotes	proteins
T25754	23709-23710	.	denotes	.
T25755	23711-23720	JJ	denotes	Wild-type
T25756	23721-23724	CC	denotes	and
T25757	23725-23731	JJ	denotes	mutant
T25758	23732-23735	NNP	denotes	Tat
T25759	23736-23744	NNS	denotes	proteins
T25760	23745-23749	VBD	denotes	were
T25761	23750-23768	VBN	denotes	immunoprecipitated
T25762	23769-23773	IN	denotes	with
T25763	23774-23783	JJ	denotes	anti-FLAG
T25764	23784-23792	NN	denotes	antibody
T25765	23794-23809	NNS	denotes	immunocomplexes
T25766	23810-23814	VBD	denotes	were
T25767	23815-23824	VBN	denotes	separated
T25768	23825-23827	IN	denotes	by
T25769	23828-23830	CD	denotes	12
T25770	23830-23831	NN	denotes	%
T25771	23832-23835	NNP	denotes	SDS
T25772	23836-23840	NNP	denotes	PAGE
T25773	23841-23844	CC	denotes	and
T25774	23845-23853	VBN	denotes	analysed
T25775	23854-23856	IN	denotes	by
T25776	23857-23864	JJ	denotes	western
T25777	23865-23873	VBG	denotes	blotting
T25778	23874-23878	IN	denotes	with
T25779	23879-23886	JJ	denotes	anti-HA
T25780	23887-23890	CC	denotes	and
T25781	23891-23900	JJ	denotes	anti-FLAG
T25782	23901-23911	NNS	denotes	antibodies
T25783	23911-23912	.	denotes	.
T25784	23913-23914	-LRB-	denotes	(
T25785	23914-23915	$	denotes	C
T25786	23915-23916	-RRB-	denotes	)
T25787	23917-23939	JJ	denotes	35S-methionine-labeled
T25788	23940-23943	NNS	denotes	p65
T25789	23944-23945	-LRB-	denotes	(
T25790	23945-23946	CD	denotes	5
T25791	23947-23949	NN	denotes	µl
T25792	23949-23950	-RRB-	denotes	)
T25793	23951-23954	VBD	denotes	was
T25794	23955-23964	VBN	denotes	incubated
T25795	23965-23969	IN	denotes	with
T25796	23970-23972	IN	denotes	in
T25797	23973-23978	NN	denotes	vitro
T25798	23979-23989	VBN	denotes	translated
T25799	23990-23998	NNP	denotes	HA-IκB-α
T25800	23999-24000	-LRB-	denotes	(
T25801	24000-24001	CD	denotes	5
T25802	24002-24004	NN	denotes	µl
T25803	24004-24005	-RRB-	denotes	)
T25804	24006-24008	IN	denotes	in
T25805	24009-24017	NN	denotes	presence
T25806	24018-24020	CC	denotes	or
T25807	24021-24028	NN	denotes	absence
T25808	24029-24031	IN	denotes	of
T25809	24032-24040	NN	denotes	FLAG-Tat
T25810	24041-24043	CC	denotes	or
T25811	24044-24052	NN	denotes	FLAG-Tat
T25812	24053-24054	NN	denotes	R
T25813	24054-24055	-LRB-	denotes	(
T25814	24055-24057	CD	denotes	49
T25815	24058-24060	CD	denotes	57
T25816	24060-24061	-RRB-	denotes	)
T25817	24061-24062	DT	denotes	A
T25818	24063-24064	-LRB-	denotes	(
T25819	24064-24065	CD	denotes	5
T25820	24065-24066	,	denotes	,
T25821	24067-24069	CD	denotes	10
T25822	24070-24072	CC	denotes	or
T25823	24073-24075	CD	denotes	20
T25824	24076-24078	NN	denotes	µl
T25825	24078-24079	-RRB-	denotes	)
T25826	24079-24080	.	denotes	.
T25827	24081-24089	NNP	denotes	HA-IκB-α
T25828	24090-24093	VBD	denotes	was
T25829	24094-24112	VBN	denotes	immunoprecipitated
T25830	24113-24117	IN	denotes	with
T25831	24118-24125	JJ	denotes	anti-HA
T25832	24126-24134	NN	denotes	antibody
T25833	24134-24135	:	denotes	;
T25834	24136-24151	NNS	denotes	immunocomplexes
T25835	24152-24156	VBD	denotes	were
T25836	24157-24166	VBN	denotes	separated
T25837	24167-24169	IN	denotes	by
T25838	24170-24172	CD	denotes	12
T25839	24172-24173	NN	denotes	%
T25840	24174-24177	NNP	denotes	SDS
T25841	24178-24182	NNP	denotes	PAGE
T25842	24183-24186	CC	denotes	and
T25843	24187-24195	VBN	denotes	analysed
T25844	24196-24198	IN	denotes	by
T25845	24199-24206	JJ	denotes	western
T25846	24207-24215	VBG	denotes	blotting
T25847	24216-24220	IN	denotes	with
T25848	24221-24228	JJ	denotes	anti-HA
T25849	24229-24232	CC	denotes	and
T25850	24233-24242	JJ	denotes	anti-FLAG
T25851	24243-24253	NNS	denotes	antibodies
T25852	24253-24254	,	denotes	,
T25853	24255-24257	CC	denotes	or
T25854	24258-24273	NN	denotes	autoradiography
T25855	24274-24275	-LRB-	denotes	(
T25856	24275-24286	NNP	denotes	35S-Met-p65
T25857	24286-24287	-RRB-	denotes	)
T25858	24287-24288	.	denotes	.
T25859	24289-24301	NNP	denotes	Densitometry
T25860	24302-24308	NNS	denotes	values
T25861	24309-24310	-LRB-	denotes	(
T25862	24310-24311	NNP	denotes	D
T25863	24311-24312	-RRB-	denotes	)
T25864	24313-24315	IN	denotes	of
T25865	24316-24319	DT	denotes	the
T25866	24320-24325	NNS	denotes	bands
T25867	24326-24330	VBD	denotes	were
T25868	24331-24340	VBN	denotes	expressed
T25869	24341-24343	IN	denotes	as
T25870	24344-24348	VBP	denotes	fold
T25871	24349-24357	NN	denotes	increase
T25872	24358-24363	IN	denotes	above
T25873	24364-24367	DT	denotes	the
T25874	24368-24375	NN	denotes	control
T25875	24376-24377	-LRB-	denotes	(
T25876	24377-24381	NN	denotes	lane
T25877	24382-24383	CD	denotes	1
T25878	24383-24384	-RRB-	denotes	)
T25879	24384-24385	.	denotes	.
T25880	24386-24387	-LRB-	denotes	(
T25881	24387-24388	NNP	denotes	D
T25882	24388-24389	-RRB-	denotes	)
T25883	24390-24393	DT	denotes	The
T25884	24394-24397	CD	denotes	p65
T25885	24398-24401	NNP	denotes	DNA
T25886	24402-24409	JJ	denotes	binding
T25887	24410-24418	NN	denotes	activity
T25888	24419-24422	VBD	denotes	was
T25889	24423-24431	VBN	denotes	analysed
T25890	24432-24434	IN	denotes	by
T25891	24435-24439	NNP	denotes	EMSA
T25892	24440-24445	VBG	denotes	using
T25893	24446-24448	IN	denotes	in
T25894	24449-24454	NN	denotes	vitro
T25895	24455-24465	VBN	denotes	translated
T25896	24466-24474	NNS	denotes	proteins
T25897	24474-24475	.	denotes	.
T25898	24476-24493	JJ	denotes	32P-labeled-NF-κB
T25899	24494-24509	JJ	denotes	double-stranded
T25900	24510-24525	NN	denotes	oligonucleotide
T25901	24526-24529	VBD	denotes	was
T25902	24530-24539	VBN	denotes	incubated
T25903	24540-24544	IN	denotes	with
T25904	24545-24548	NNS	denotes	p65
T25905	24549-24550	-LRB-	denotes	(
T25906	24550-24553	CD	denotes	0.5
T25907	24554-24556	NN	denotes	µl
T25908	24556-24557	-RRB-	denotes	)
T25909	24558-24560	IN	denotes	in
T25910	24561-24569	NN	denotes	presence
T25911	24570-24572	CC	denotes	or
T25912	24573-24580	NN	denotes	absence
T25913	24581-24583	IN	denotes	of
T25914	24584-24592	NNP	denotes	HA-IκB-α
T25915	24593-24594	-LRB-	denotes	(
T25916	24594-24595	CD	denotes	1
T25917	24596-24598	NN	denotes	µl
T25918	24598-24599	-RRB-	denotes	)
T25919	24599-24600	,	denotes	,
T25920	24601-24609	JJ	denotes	FLAG-Tat
T25921	24610-24612	CC	denotes	or
T25922	24613-24621	JJ	denotes	FLAG-Tat
T25923	24622-24623	NN	denotes	R
T25924	24623-24624	-LRB-	denotes	(
T25925	24624-24626	CD	denotes	49
T25926	24627-24629	CD	denotes	57
T25927	24629-24630	-RRB-	denotes	)
T25928	24630-24631	DT	denotes	A
T25929	24632-24633	-LRB-	denotes	(
T25930	24633-24634	CD	denotes	5
T25931	24635-24638	CC	denotes	and
T25932	24639-24641	CD	denotes	10
T25933	24642-24644	NN	denotes	µl
T25934	24644-24645	-RRB-	denotes	)
T25935	24645-24646	:	denotes	;
T25936	24647-24658	NN	denotes	competition
T25937	24659-24661	IN	denotes	of
T25938	24662-24665	NNP	denotes	DNA
T25939	24666-24673	JJ	denotes	binding
T25940	24674-24677	VBD	denotes	was
T25941	24678-24687	VBN	denotes	performed
T25942	24688-24692	IN	denotes	with
T25943	24693-24695	CD	denotes	10
T25944	24696-24697	:	denotes	-
T25945	24698-24700	RP	denotes	up
T25946	24701-24703	TO	denotes	to
T25947	24704-24712	JJ	denotes	100-fold
T25948	24713-24718	NN	denotes	molar
T25949	24719-24725	NN	denotes	excess
T25950	24726-24728	IN	denotes	of
T25951	24729-24738	JJ	denotes	unlabeled
T25952	24739-24744	JJ	denotes	NF-κB
T25953	24745-24760	JJ	denotes	double-stranded
T25954	24761-24776	NN	denotes	oligonucleotide
T25955	24776-24777	.	denotes	.
T25956	24778-24789	NNP	denotes	DNA/protein
T25957	24790-24799	NNS	denotes	complexes
T25958	24800-24804	VBD	denotes	were
T25959	24805-24808	VBN	denotes	run
T25960	24809-24811	IN	denotes	on
T25961	24812-24813	CD	denotes	6
T25962	24813-24814	NN	denotes	%
T25963	24815-24819	NN	denotes	PAGE
T25964	24820-24823	NNP	denotes	TBE
T25965	24824-24827	CC	denotes	and
T25966	24828-24836	VBN	denotes	analysed
T25967	24837-24839	IN	denotes	by
T25968	24840-24855	NN	denotes	autoradiography
T25969	24855-24856	.	denotes	.
T25970	24857-24858	-LRB-	denotes	(
T25971	24858-24859	NNP	denotes	E
T25972	24859-24860	-RRB-	denotes	)
T25973	24861-24864	FW	denotes	p50
T25974	24864-24865	FW	denotes	−
T25975	24865-24866	FW	denotes	/
T25976	24866-24867	FW	denotes	−
T25977	24867-24870	FW	denotes	p65
T25978	24870-24871	FW	denotes	−
T25979	24871-24872	FW	denotes	/
T25980	24872-24873	FW	denotes	−
T25981	24873-24877	NNS	denotes	MEFs
T25982	24878-24879	-LRB-	denotes	(
T25983	24879-24880	CD	denotes	3
T25984	24881-24882	CD	denotes	×
T25985	24883-24886	CD	denotes	105
T25986	24887-24892	NNS	denotes	cells
T25987	24892-24893	-RRB-	denotes	)
T25988	24894-24898	VBD	denotes	were
T25989	24899-24910	VBN	denotes	transfected
T25990	24911-24915	IN	denotes	with
T25991	24916-24922	NNP	denotes	pκBLuc
T25992	24923-24924	-LRB-	denotes	(
T25993	24924-24927	CD	denotes	0.5
T25994	24928-24930	NN	denotes	µg
T25995	24930-24931	-RRB-	denotes	)
T25996	24932-24935	CC	denotes	and
T25997	24936-24945	JJ	denotes	pSV-β-Gal
T25998	24946-24947	-LRB-	denotes	(
T25999	24947-24950	CD	denotes	0.1
T26000	24951-24953	NN	denotes	µg
T26001	24953-24954	-RRB-	denotes	)
T26002	24955-24959	IN	denotes	with
T26003	24960-24962	CC	denotes	or
T26004	24963-24970	IN	denotes	without
T26005	24971-24980	NNP	denotes	pRc/CMV-p
T26006	24980-24982	CD	denotes	65
T26007	24983-24984	-LRB-	denotes	(
T26008	24984-24987	CD	denotes	0.5
T26009	24988-24990	NN	denotes	µg
T26010	24990-24991	-RRB-	denotes	)
T26011	24991-24992	,	denotes	,
T26012	24993-25007	NN	denotes	pCMV4-HA-IκB-α
T26013	25008-25009	-LRB-	denotes	(
T26014	25009-25012	CD	denotes	0.5
T26015	25013-25015	NN	denotes	µg
T26016	25015-25016	-RRB-	denotes	)
T26017	25016-25017	,	denotes	,
T26018	25018-25029	NN	denotes	p3xFLAG-Tat
T26019	25030-25032	CC	denotes	or
T26020	25033-25044	NN	denotes	p3xFLAG-Tat
T26021	25045-25046	NN	denotes	R
T26022	25046-25047	-LRB-	denotes	(
T26023	25047-25049	CD	denotes	49
T26024	25050-25052	CD	denotes	57
T26025	25052-25053	-RRB-	denotes	)
T26026	25053-25054	DT	denotes	A
T26027	25055-25056	-LRB-	denotes	(
T26028	25056-25059	CD	denotes	0.5
T26029	25059-25060	,	denotes	,
T26030	25061-25062	CD	denotes	1
T26031	25063-25066	CC	denotes	and
T26032	25067-25068	CD	denotes	2
T26033	25069-25071	NN	denotes	µg
T26034	25071-25072	-RRB-	denotes	)
T26035	25072-25073	.	denotes	.
T26036	25074-25077	DT	denotes	The
T26037	25078-25088	NN	denotes	luciferase
T26038	25089-25097	NN	denotes	activity
T26039	25098-25101	VBD	denotes	was
T26040	25102-25110	VBN	denotes	measured
T26041	25111-25113	IN	denotes	in
T26042	25114-25118	NN	denotes	cell
T26043	25119-25127	NNS	denotes	extracts
T26044	25128-25132	JJ	denotes	48-h
T26045	25133-25150	NN	denotes	post-transfection
T26046	25151-25154	CC	denotes	and
T26047	25155-25165	VBN	denotes	normalized
T26048	25166-25168	TO	denotes	to
T26049	25169-25184	JJ	denotes	β-galactosidase
T26050	25185-25193	NN	denotes	activity
T26051	25193-25194	.	denotes	.
T26052	25195-25199	VB	denotes	Fold
T26053	25200-25210	NN	denotes	activation
T26054	25211-25214	VBD	denotes	was
T26055	25215-25225	VBN	denotes	calculated
T26056	25226-25234	JJ	denotes	relative
T26057	25235-25237	TO	denotes	to
T26058	25238-25250	NN	denotes	transfection
T26059	25251-25253	IN	denotes	of
T26060	25254-25257	DT	denotes	the
T26061	25258-25264	NNP	denotes	pκBLuc
T26062	25265-25272	NN	denotes	plasmid
T26063	25273-25278	RB	denotes	alone
T26064	25278-25279	.	denotes	.
T26065	25280-25286	NNS	denotes	Values
T26066	25287-25288	-LRB-	denotes	(
T26067	25288-25292	JJ	denotes	mean
T26068	25293-25294	NN	denotes	±
T26069	25295-25297	NNP	denotes	SE
T26070	25297-25298	,	denotes	,
T26071	25299-25300	JJ	denotes	n
T26072	25301-25302	SYM	denotes	=
T26073	25303-25304	LS	denotes	3
T26074	25304-25305	-RRB-	denotes	)
T26075	25306-25309	VBP	denotes	are
T26076	25310-25315	VBN	denotes	shown
T26077	25315-25316	.	denotes	.
T26078	25317-25319	IN	denotes	As
T26079	25320-25327	NN	denotes	control
T26080	25328-25330	IN	denotes	of
T26081	25331-25338	NN	denotes	protein
T26082	25339-25349	NN	denotes	expression
T26083	25349-25350	,	denotes	,
T26084	25351-25359	NNS	denotes	aliquots
T26085	25360-25362	IN	denotes	of
T26086	25363-25367	NN	denotes	cell
T26087	25368-25376	NNS	denotes	extracts
T26088	25377-25378	-LRB-	denotes	(
T26089	25378-25380	CD	denotes	20
T26090	25381-25383	NN	denotes	µg
T26091	25383-25384	-RRB-	denotes	)
T26092	25385-25389	VBD	denotes	were
T26093	25390-25398	VBN	denotes	analysed
T26094	25399-25401	IN	denotes	by
T26095	25402-25409	JJ	denotes	western
T26096	25410-25418	VBG	denotes	blotting
T26097	25419-25423	IN	denotes	with
T26098	25424-25432	JJ	denotes	anti-p65
T26099	25432-25433	,	denotes	,
T26100	25434-25441	JJ	denotes	anti-HA
T26101	25441-25442	,	denotes	,
T26102	25443-25452	JJ	denotes	anti-FLAG
T26103	25453-25456	CC	denotes	and
T26104	25457-25471	JJ	denotes	anti-γ-tubulin
T26105	25472-25482	NNS	denotes	antibodies
T26106	25482-25483	.	denotes	.
T26585	27379-27382	NNP	denotes	Tat
T26586	27383-27393	NNS	denotes	associates
T26587	27394-27398	IN	denotes	with
T26588	27399-27402	CD	denotes	p65
T26589	27403-27413	VBG	denotes	increasing
T26590	27414-27417	PRP$	denotes	its
T26591	27418-27430	JJ	denotes	DNA-affinity
T26592	27431-27438	JJ	denotes	binding
T26593	27438-27439	.	denotes	.
T26594	27440-27441	-LRB-	denotes	(
T26595	27441-27442	NNP	denotes	A
T26596	27442-27443	-RRB-	denotes	)
T26597	27444-27448	NNP	denotes	HeLa
T26598	27449-27454	NNS	denotes	cells
T26599	27455-27459	VBD	denotes	were
T26600	27460-27471	VBN	denotes	transfected
T26601	27472-27476	IN	denotes	with
T26602	27477-27484	NNP	denotes	pRc/CMV
T26603	27484-27486	CD	denotes	-3
T26604	27486-27493	NNP	denotes	xHA-p65
T26605	27494-27495	-LRB-	denotes	(
T26606	27495-27496	CD	denotes	5
T26607	27497-27499	NN	denotes	µg
T26608	27499-27500	-RRB-	denotes	)
T26609	27501-27503	IN	denotes	in
T26610	27504-27512	NN	denotes	presence
T26611	27513-27515	CC	denotes	or
T26612	27516-27523	NN	denotes	absence
T26613	27524-27526	IN	denotes	of
T26614	27527-27538	NN	denotes	p3xFLAG-Tat
T26615	27538-27539	,	denotes	,
T26616	27540-27551	NN	denotes	p3xFLAG-Tat
T26617	27552-27553	NNP	denotes	T
T26618	27553-27554	,	denotes	,
T26619	27554-27555	NNP	denotes	N
T26620	27555-27556	-LRB-	denotes	(
T26621	27556-27561	CD	denotes	23,24
T26622	27561-27562	-RRB-	denotes	)
T26623	27562-27563	DT	denotes	A
T26624	27563-27564	,	denotes	,
T26625	27565-27576	JJ	denotes	p3xFLAG-Tat
T26626	27577-27578	NNP	denotes	K
T26627	27578-27579	-LRB-	denotes	(
T26628	27579-27584	CD	denotes	50,51
T26629	27584-27585	-RRB-	denotes	)
T26630	27585-27586	DT	denotes	A
T26631	27586-27587	,	denotes	,
T26632	27588-27599	JJ	denotes	p3xFLAG-Tat
T26633	27600-27601	NN	denotes	R
T26634	27601-27602	-LRB-	denotes	(
T26635	27602-27604	CD	denotes	49
T26636	27605-27607	CD	denotes	57
T26637	27607-27608	-RRB-	denotes	)
T26638	27608-27609	DT	denotes	A
T26639	27609-27610	,	denotes	,
T26640	27611-27613	CC	denotes	or
T26641	27614-27625	NN	denotes	p3xFLAG-Tat
T26642	27626-27627	NNP	denotes	C
T26643	27627-27628	-LRB-	denotes	(
T26644	27628-27636	CD	denotes	22,25,27
T26645	27636-27637	-RRB-	denotes	)
T26646	27637-27638	DT	denotes	A
T26647	27639-27640	-LRB-	denotes	(
T26648	27640-27641	CD	denotes	5
T26649	27642-27644	NN	denotes	µg
T26650	27644-27645	-RRB-	denotes	)
T26651	27645-27646	.	denotes	.
T26652	27647-27655	JJ	denotes	FLAG-Tat
T26653	27656-27664	NNS	denotes	proteins
T26654	27665-27669	VBD	denotes	were
T26655	27670-27688	VBN	denotes	immunoprecipitated
T26656	27689-27693	IN	denotes	with
T26657	27694-27703	JJ	denotes	anti-FLAG
T26658	27704-27712	NN	denotes	antibody
T26659	27712-27713	:	denotes	;
T26660	27714-27729	NNS	denotes	immunocomplexes
T26661	27730-27734	VBD	denotes	were
T26662	27735-27744	VBN	denotes	separated
T26663	27745-27747	IN	denotes	by
T26664	27748-27750	CD	denotes	12
T26665	27750-27751	NN	denotes	%
T26666	27752-27755	NNP	denotes	SDS
T26667	27756-27760	NNP	denotes	PAGE
T26668	27761-27764	CC	denotes	and
T26669	27765-27773	VBN	denotes	analysed
T26670	27774-27776	IN	denotes	by
T26671	27777-27784	JJ	denotes	western
T26672	27785-27793	VBG	denotes	blotting
T26673	27794-27798	IN	denotes	with
T26674	27799-27808	JJ	denotes	anti-FLAG
T26675	27809-27811	CC	denotes	or
T26676	27812-27819	JJ	denotes	anti-HA
T26677	27820-27830	NNS	denotes	antibodies
T26678	27830-27831	.	denotes	.
T26679	27832-27833	-LRB-	denotes	(
T26680	27833-27834	NNP	denotes	B
T26681	27834-27835	-RRB-	denotes	)
T26682	27836-27838	IN	denotes	In
T26683	27839-27844	NN	denotes	vitro
T26684	27845-27855	VBN	denotes	translated
T26685	27856-27859	NNS	denotes	p65
T26686	27860-27861	-LRB-	denotes	(
T26687	27861-27862	CD	denotes	5
T26688	27863-27865	NN	denotes	µl
T26689	27865-27866	-RRB-	denotes	)
T26690	27867-27870	VBD	denotes	was
T26691	27871-27880	VBN	denotes	incubated
T26692	27881-27885	IN	denotes	with
T26693	27886-27893	NNP	denotes	GST-Tat
T26694	27893-27894	,	denotes	,
T26695	27895-27902	NNP	denotes	GST-Tat
T26696	27903-27904	NNP	denotes	T
T26697	27904-27905	,	denotes	,
T26698	27905-27906	NNP	denotes	N
T26699	27906-27907	-LRB-	denotes	(
T26700	27907-27912	CD	denotes	23,24
T26701	27912-27913	-RRB-	denotes	)
T26702	27913-27914	DT	denotes	A
T26703	27914-27915	,	denotes	,
T26704	27916-27923	NNP	denotes	GST-Tat
T26705	27924-27925	NNP	denotes	K
T26706	27925-27926	-LRB-	denotes	(
T26707	27926-27931	CD	denotes	50,51
T26708	27931-27932	-RRB-	denotes	)
T26709	27932-27933	DT	denotes	A
T26710	27933-27934	,	denotes	,
T26711	27935-27942	NNP	denotes	GST-Tat
T26712	27943-27944	NNP	denotes	R
T26713	27944-27945	-LRB-	denotes	(
T26714	27945-27947	CD	denotes	49
T26715	27948-27950	CD	denotes	57
T26716	27950-27951	-RRB-	denotes	)
T26717	27951-27952	DT	denotes	A
T26718	27952-27953	,	denotes	,
T26719	27954-27961	NNP	denotes	GST-Tat
T26720	27962-27963	NNP	denotes	C
T26721	27963-27964	-LRB-	denotes	(
T26722	27964-27972	CD	denotes	22,25,27
T26723	27972-27973	-RRB-	denotes	)
T26724	27973-27974	DT	denotes	A
T26725	27974-27975	,	denotes	,
T26726	27976-27978	CC	denotes	or
T26727	27979-27982	NNP	denotes	GST
T26728	27983-27984	-LRB-	denotes	(
T26729	27984-27985	CD	denotes	5
T26730	27986-27988	NN	denotes	µg
T26731	27988-27989	-RRB-	denotes	)
T26732	27990-28000	VBN	denotes	conjugated
T26733	28001-28005	IN	denotes	with
T26734	28006-28027	NNP	denotes	Glutathione-Sepharose
T26735	28027-28028	.	denotes	.
T26736	28029-28036	NN	denotes	Protein
T26737	28037-28046	NNS	denotes	complexes
T26738	28047-28051	VBD	denotes	were
T26739	28052-28061	VBN	denotes	recovered
T26740	28062-28064	IN	denotes	by
T26741	28065-28073	NNP	denotes	GST-pull
T26742	28074-28078	RB	denotes	down
T26743	28078-28079	,	denotes	,
T26744	28080-28089	VBN	denotes	separated
T26745	28090-28092	IN	denotes	by
T26746	28093-28095	CD	denotes	12
T26747	28095-28096	NN	denotes	%
T26748	28097-28100	NNP	denotes	SDS
T26749	28101-28105	NNP	denotes	PAGE
T26750	28105-28106	,	denotes	,
T26751	28107-28110	CC	denotes	and
T26752	28111-28119	VBN	denotes	analysed
T26753	28120-28122	IN	denotes	by
T26754	28123-28130	JJ	denotes	western
T26755	28131-28139	VBG	denotes	blotting
T26756	28140-28144	IN	denotes	with
T26757	28145-28153	JJ	denotes	anti-p65
T26758	28154-28156	CC	denotes	or
T26759	28157-28165	JJ	denotes	anti-GST
T26760	28166-28176	NNS	denotes	antibodies
T26761	28176-28177	.	denotes	.
T26762	28178-28179	-LRB-	denotes	(
T26763	28179-28180	$	denotes	C
T26764	28180-28181	-RRB-	denotes	)
T26765	28182-28191	JJ	denotes	Schematic
T26766	28192-28206	NN	denotes	representation
T26767	28207-28209	IN	denotes	of
T26768	28210-28219	JJ	denotes	wild-type
T26769	28220-28223	CC	denotes	and
T26770	28224-28230	JJ	denotes	mutant
T26771	28231-28234	NNS	denotes	p65
T26772	28235-28243	NNS	denotes	proteins
T26773	28243-28244	.	denotes	.
T26774	28245-28246	-LRB-	denotes	(
T26775	28246-28247	NNP	denotes	D
T26776	28247-28248	-RRB-	denotes	)
T26777	28249-28253	NNP	denotes	HeLa
T26778	28254-28259	NNS	denotes	cells
T26779	28260-28264	VBD	denotes	were
T26780	28265-28276	VBN	denotes	transfected
T26781	28277-28281	IN	denotes	with
T26782	28282-28293	NN	denotes	p3xFLAG-Tat
T26783	28294-28295	-LRB-	denotes	(
T26784	28295-28296	CD	denotes	5
T26785	28297-28299	NN	denotes	µg
T26786	28299-28300	-RRB-	denotes	)
T26787	28301-28303	IN	denotes	in
T26788	28304-28307	DT	denotes	the
T26789	28308-28316	NN	denotes	presence
T26790	28317-28319	CC	denotes	or
T26791	28320-28327	NN	denotes	absence
T26792	28328-28330	IN	denotes	of
T26793	28331-28338	NNP	denotes	pRc/CMV
T26794	28338-28340	CD	denotes	-3
T26795	28340-28347	NNP	denotes	xHA-p65
T26796	28347-28348	,	denotes	,
T26797	28349-28356	NNP	denotes	pRc/CMV
T26798	28356-28358	CD	denotes	-3
T26799	28358-28367	NN	denotes	xHA-p65ΔC
T26800	28368-28369	-LRB-	denotes	(
T26801	28369-28370	CD	denotes	1
T26802	28371-28374	CD	denotes	318
T26803	28374-28375	-RRB-	denotes	)
T26804	28375-28376	,	denotes	,
T26805	28377-28379	CC	denotes	or
T26806	28380-28387	NNP	denotes	pRc/CMV
T26807	28387-28389	CD	denotes	-3
T26808	28389-28398	NNP	denotes	xHA-p65ΔN
T26809	28399-28400	-LRB-	denotes	(
T26810	28400-28403	CD	denotes	122
T26811	28404-28407	CD	denotes	551
T26812	28407-28408	-RRB-	denotes	)
T26813	28409-28410	-LRB-	denotes	(
T26814	28410-28411	CD	denotes	5
T26815	28412-28414	NN	denotes	µg
T26816	28414-28415	-RRB-	denotes	)
T26817	28415-28416	.	denotes	.
T26818	28417-28426	JJ	denotes	Wild-type
T26819	28427-28430	CC	denotes	and
T26820	28431-28437	JJ	denotes	mutant
T26821	28438-28444	NN	denotes	HA-p65
T26822	28445-28453	NNS	denotes	proteins
T26823	28454-28458	VBD	denotes	were
T26824	28459-28477	VBN	denotes	immunoprecipitated
T26825	28478-28482	IN	denotes	with
T26826	28483-28490	JJ	denotes	anti-HA
T26827	28491-28499	NN	denotes	antibody
T26828	28499-28500	:	denotes	;
T26829	28501-28516	NNS	denotes	immunocomplexes
T26830	28517-28521	VBD	denotes	were
T26831	28522-28531	VBN	denotes	separated
T26832	28532-28534	IN	denotes	by
T26833	28535-28537	CD	denotes	12
T26834	28537-28538	NN	denotes	%
T26835	28539-28542	NNP	denotes	SDS
T26836	28543-28547	NNP	denotes	PAGE
T26837	28548-28551	CC	denotes	and
T26838	28552-28560	VBN	denotes	analysed
T26839	28561-28563	IN	denotes	by
T26840	28564-28571	JJ	denotes	western
T26841	28572-28580	VBG	denotes	blotting
T26842	28581-28585	IN	denotes	with
T26843	28586-28595	JJ	denotes	anti-FLAG
T26844	28596-28598	CC	denotes	or
T26845	28599-28606	JJ	denotes	anti-HA
T26846	28607-28617	NNS	denotes	antibodies
T26847	28617-28618	.	denotes	.
T26848	28619-28620	-LRB-	denotes	(
T26849	28620-28621	NNP	denotes	E
T26850	28621-28622	-RRB-	denotes	)
T26851	28623-28634	JJ	denotes	Recombinant
T26852	28635-28638	NNS	denotes	p65
T26853	28639-28646	NN	denotes	protein
T26854	28647-28648	-LRB-	denotes	(
T26855	28648-28651	CD	denotes	100
T26856	28652-28654	NN	denotes	ng
T26857	28654-28655	:	denotes	;
T26858	28656-28662	NNP	denotes	Active
T26859	28663-28668	NNP	denotes	Motif
T26860	28669-28677	NNP	denotes	Carlsbad
T26861	28677-28678	,	denotes	,
T26862	28679-28681	NNP	denotes	CA
T26863	28681-28682	,	denotes	,
T26864	28683-28686	NNP	denotes	USA
T26865	28686-28687	-RRB-	denotes	)
T26866	28688-28691	VBD	denotes	was
T26867	28692-28694	CD	denotes	20
T26868	28695-28698	NN	denotes	min
T26869	28699-28708	VBN	denotes	incubated
T26870	28709-28713	IN	denotes	with
T26871	28714-28725	JJ	denotes	32P-labeled
T26872	28726-28731	JJ	denotes	NF-κB
T26873	28732-28747	JJ	denotes	double-stranded
T26874	28748-28763	NN	denotes	oligonucleotide
T26875	28764-28766	IN	denotes	in
T26876	28767-28775	NN	denotes	presence
T26877	28776-28778	CC	denotes	or
T26878	28779-28786	NN	denotes	absence
T26879	28787-28789	IN	denotes	of
T26880	28790-28792	IN	denotes	in
T26881	28793-28798	NN	denotes	vitro
T26882	28799-28809	VBN	denotes	translated
T26883	28810-28818	NNP	denotes	FLAG-Tat
T26884	28819-28821	CC	denotes	or
T26885	28822-28830	NNP	denotes	FLAG-Tat
T26886	28831-28832	NNP	denotes	C
T26887	28832-28833	-LRB-	denotes	(
T26888	28833-28841	CD	denotes	22,25,27
T26889	28841-28842	-RRB-	denotes	)
T26890	28842-28843	DT	denotes	A
T26891	28844-28845	-LRB-	denotes	(
T26892	28845-28846	CD	denotes	5
T26893	28847-28849	NN	denotes	µl
T26894	28849-28850	-RRB-	denotes	)
T26895	28850-28851	:	denotes	;
T26896	28852-28864	NNS	denotes	competitions
T26897	28865-28869	VBD	denotes	were
T26898	28870-28879	VBN	denotes	performed
T26899	28880-28884	IN	denotes	with
T26900	28885-28889	CD	denotes	1.25
T26901	28889-28890	:	denotes	-
T26902	28891-28893	RP	denotes	up
T26903	28894-28896	TO	denotes	to
T26904	28897-28904	JJ	denotes	40-fold
T26905	28905-28910	NN	denotes	molar
T26906	28911-28917	NN	denotes	excess
T26907	28918-28920	IN	denotes	of
T26908	28921-28930	JJ	denotes	unlabeled
T26909	28931-28946	NN	denotes	oligonucleotide
T26910	28946-28947	.	denotes	.
T26911	28948-28959	NNP	denotes	DNA/protein
T26912	28960-28969	NNS	denotes	complexes
T26913	28970-28974	VBD	denotes	were
T26914	28975-28984	VBN	denotes	separated
T26915	28985-28987	IN	denotes	by
T26916	28988-28989	CD	denotes	6
T26917	28989-28990	NN	denotes	%
T26918	28991-28995	NN	denotes	PAGE
T26919	28996-28998	IN	denotes	in
T26920	28999-29002	CD	denotes	0.5
T26921	29003-29004	CD	denotes	×
T26922	29005-29008	NNP	denotes	TBE
T26923	29009-29015	NN	denotes	buffer
T26924	29016-29019	CC	denotes	and
T26925	29020-29028	VBN	denotes	analysed
T26926	29029-29031	IN	denotes	by
T26927	29032-29047	NN	denotes	autoradiography
T26928	29047-29048	.	denotes	.
T26929	29049-29050	-LRB-	denotes	(
T26930	29050-29051	NN	denotes	F
T26931	29051-29052	-RRB-	denotes	)
T26932	29053-29065	NN	denotes	Densitometry
T26933	29066-29068	IN	denotes	of
T26934	29069-29080	NNS	denotes	band-shifts
T26935	29081-29086	VBN	denotes	shown
T26936	29087-29089	IN	denotes	in
T26937	29090-29092	NNP	denotes	E.
T27629	31394-31397	NNP	denotes	Tat
T27630	31398-31407	VBZ	denotes	activates
T27631	31408-31411	DT	denotes	the
T27632	31412-31425	JJ	denotes	p65-dependent
T27633	31426-31436	NN	denotes	expression
T27634	31437-31439	IN	denotes	of
T27635	31440-31456	JJ	denotes	NF-κB-responsive
T27636	31457-31462	NNS	denotes	genes
T27637	31463-31465	IN	denotes	by
T27638	31466-31475	VBG	denotes	occupying
T27639	31476-31479	DT	denotes	the
T27640	31480-31485	JJ	denotes	NF-κB
T27641	31486-31495	NNS	denotes	enhancers
T27642	31496-31499	CC	denotes	and
T27643	31500-31509	VBG	denotes	promoting
T27644	31510-31513	DT	denotes	the
T27645	31514-31517	CD	denotes	p65
T27646	31518-31529	NN	denotes	recruitment
T27647	31530-31534	IN	denotes	with
T27648	31535-31540	JJ	denotes	IκB-α
T27649	31541-31553	NN	denotes	displacement
T27650	31553-31554	.	denotes	.
T27651	31555-31559	NNP	denotes	HeLa
T27652	31560-31565	NNS	denotes	cells
T27653	31566-31567	-LRB-	denotes	(
T27654	31567-31568	CD	denotes	5
T27655	31569-31570	CD	denotes	×
T27656	31571-31574	CD	denotes	106
T27657	31574-31575	-RRB-	denotes	)
T27658	31576-31580	VBD	denotes	were
T27659	31581-31592	VBN	denotes	transfected
T27660	31593-31597	IN	denotes	with
T27661	31598-31609	NN	denotes	p3xFLAG-Tat
T27662	31609-31610	,	denotes	,
T27663	31611-31622	NN	denotes	p3xFLAG-Tat
T27664	31623-31624	NNP	denotes	C
T27665	31624-31625	-LRB-	denotes	(
T27666	31625-31633	CD	denotes	22,25,27
T27667	31633-31634	-RRB-	denotes	)
T27668	31634-31635	,	denotes	,
T27669	31636-31647	JJ	denotes	p3xFLAG-Tat
T27670	31648-31649	NN	denotes	R
T27671	31649-31650	-LRB-	denotes	(
T27672	31650-31652	CD	denotes	49
T27673	31653-31655	CD	denotes	57
T27674	31655-31656	-RRB-	denotes	)
T27675	31657-31659	CC	denotes	or
T27676	31660-31665	JJ	denotes	empty
T27677	31666-31672	NN	denotes	vector
T27678	31673-31674	-LRB-	denotes	(
T27679	31674-31676	CD	denotes	10
T27680	31677-31679	NN	denotes	µg
T27681	31679-31680	-RRB-	denotes	)
T27682	31680-31681	:	denotes	;
T27683	31682-31685	IN	denotes	for
T27684	31686-31689	CD	denotes	p65
T27685	31690-31693	NNP	denotes	RNA
T27686	31694-31706	NN	denotes	interference
T27687	31706-31707	,	denotes	,
T27688	31708-31713	NNS	denotes	cells
T27689	31714-31715	-LRB-	denotes	(
T27690	31715-31716	CD	denotes	5
T27691	31717-31718	CD	denotes	×
T27692	31719-31722	CD	denotes	106
T27693	31722-31723	-RRB-	denotes	)
T27694	31724-31728	VBD	denotes	were
T27695	31729-31740	VBN	denotes	transfected
T27696	31741-31745	IN	denotes	with
T27697	31746-31757	NN	denotes	p3xFLAG-Tat
T27698	31758-31759	-LRB-	denotes	(
T27699	31759-31761	CD	denotes	10
T27700	31762-31764	NN	denotes	µg
T27701	31764-31765	-RRB-	denotes	)
T27702	31766-31769	CC	denotes	and
T27703	31770-31775	NNP	denotes	siRNA
T27704	31776-31779	CD	denotes	p65
T27705	31780-31782	CC	denotes	or
T27706	31783-31788	JJ	denotes	siRNA
T27707	31789-31796	NN	denotes	control
T27708	31797-31798	-LRB-	denotes	(
T27709	31798-31801	CD	denotes	200
T27710	31802-31806	NN	denotes	pmol
T27711	31806-31807	-RRB-	denotes	)
T27712	31807-31808	.	denotes	.
T27713	31809-31825	CD	denotes	Twenty-four-hour
T27714	31826-31843	JJ	denotes	post-transfection
T27715	31843-31844	,	denotes	,
T27716	31845-31850	JJ	denotes	total
T27717	31851-31854	NNP	denotes	RNA
T27718	31855-31858	VBD	denotes	was
T27719	31859-31868	VBN	denotes	extracted
T27720	31869-31872	CC	denotes	and
T27721	31873-31881	VBN	denotes	analysed
T27722	31882-31884	IN	denotes	by
T27723	31885-31894	JJ	denotes	real-time
T27724	31895-31898	NNP	denotes	PCR
T27725	31899-31901	TO	denotes	to
T27726	31902-31910	VB	denotes	evaluate
T27727	31911-31914	DT	denotes	the
T27728	31915-31925	NN	denotes	expression
T27729	31926-31928	IN	denotes	of
T27730	31929-31935	NNP	denotes	MIP-1α
T27731	31936-31937	-LRB-	denotes	(
T27732	31937-31938	NNP	denotes	A
T27733	31938-31939	-RRB-	denotes	)
T27734	31939-31940	,	denotes	,
T27735	31941-31945	NNP	denotes	CSF3
T27736	31946-31947	-LRB-	denotes	(
T27737	31947-31948	NNP	denotes	B
T27738	31948-31949	-RRB-	denotes	)
T27739	31949-31950	,	denotes	,
T27740	31951-31954	NNP	denotes	LTA
T27741	31955-31956	-LRB-	denotes	(
T27742	31956-31957	NNP	denotes	C
T27743	31957-31958	-RRB-	denotes	)
T27744	31958-31959	,	denotes	,
T27745	31960-31966	NNP	denotes	NFKBIA
T27746	31967-31968	-LRB-	denotes	(
T27747	31968-31969	NNP	denotes	D
T27748	31969-31970	-RRB-	denotes	)
T27749	31970-31971	,	denotes	,
T27750	31972-31976	NNP	denotes	TLR2
T27751	31977-31978	-LRB-	denotes	(
T27752	31978-31979	NNP	denotes	E
T27753	31979-31980	-RRB-	denotes	)
T27754	31980-31981	,	denotes	,
T27755	31982-31987	NNP	denotes	GAPDH
T27756	31988-31989	-LRB-	denotes	(
T27757	31989-31990	NN	denotes	F
T27758	31990-31991	-RRB-	denotes	)
T27759	31991-31992	,	denotes	,
T27760	31993-31997	NNP	denotes	ACTB
T27761	31998-31999	-LRB-	denotes	(
T27762	31999-32000	NNP	denotes	G
T27763	32000-32001	-RRB-	denotes	)
T27764	32002-32007	NNS	denotes	genes
T27765	32007-32008	.	denotes	.
T27766	32009-32010	-LRB-	denotes	(
T27767	32010-32011	NNP	denotes	H
T27768	32011-32012	-RRB-	denotes	)
T27769	32013-32017	NNP	denotes	HeLa
T27770	32018-32023	NNS	denotes	cells
T27771	32024-32025	-LRB-	denotes	(
T27772	32025-32026	CD	denotes	5
T27773	32027-32028	CD	denotes	×
T27774	32029-32032	CD	denotes	106
T27775	32032-32033	-RRB-	denotes	)
T27776	32034-32038	VBD	denotes	were
T27777	32039-32050	VBN	denotes	transfected
T27778	32051-32055	IN	denotes	with
T27779	32056-32067	NN	denotes	p3xFLAG-Tat
T27780	32067-32068	,	denotes	,
T27781	32069-32071	CC	denotes	or
T27782	32072-32077	JJ	denotes	empty
T27783	32078-32084	NN	denotes	vector
T27784	32085-32086	-LRB-	denotes	(
T27785	32086-32088	CD	denotes	10
T27786	32089-32091	NN	denotes	µg
T27787	32091-32092	-RRB-	denotes	)
T27788	32093-32096	CC	denotes	and
T27789	32097-32099	CD	denotes	48
T27790	32100-32101	NN	denotes	h
T27791	32102-32107	RB	denotes	later
T27792	32108-32112	NNP	denotes	ChIP
T27793	32113-32116	VBD	denotes	was
T27794	32117-32126	VBN	denotes	performed
T27795	32127-32131	IN	denotes	with
T27796	32132-32140	JJ	denotes	anti-p65
T27797	32141-32149	NN	denotes	antibody
T27798	32149-32150	.	denotes	.
T27799	32151-32160	JJ	denotes	Real-time
T27800	32161-32164	NNP	denotes	PCR
T27801	32165-32168	VBD	denotes	was
T27802	32169-32178	VBN	denotes	performed
T27803	32179-32183	IN	denotes	with
T27804	32184-32191	NNS	denotes	primers
T27805	32192-32200	JJ	denotes	specific
T27806	32201-32204	IN	denotes	for
T27807	32205-32208	DT	denotes	the
T27808	32209-32218	VBN	denotes	indicated
T27809	32219-32228	NNS	denotes	promoters
T27810	32228-32229	.	denotes	.
T27811	32230-32231	-LRB-	denotes	(
T27812	32231-32232	PRP	denotes	I
T27813	32232-32233	-RRB-	denotes	)
T27814	32234-32238	NNP	denotes	HeLa
T27815	32239-32244	NNS	denotes	cells
T27816	32245-32246	-LRB-	denotes	(
T27817	32246-32247	CD	denotes	5
T27818	32248-32249	CD	denotes	×
T27819	32250-32253	CD	denotes	106
T27820	32253-32254	-RRB-	denotes	)
T27821	32255-32259	VBD	denotes	were
T27822	32260-32271	VBN	denotes	transfected
T27823	32272-32276	IN	denotes	with
T27824	32277-32282	JJ	denotes	empty
T27825	32283-32289	NN	denotes	vector
T27826	32289-32290	,	denotes	,
T27827	32291-32293	CC	denotes	or
T27828	32294-32305	NN	denotes	p3xFLAG-Tat
T27829	32306-32307	-LRB-	denotes	(
T27830	32307-32309	CD	denotes	10
T27831	32310-32312	NN	denotes	µg
T27832	32312-32313	-RRB-	denotes	)
T27833	32314-32316	IN	denotes	in
T27834	32317-32325	NN	denotes	presence
T27835	32326-32328	CC	denotes	or
T27836	32329-32336	NN	denotes	absence
T27837	32337-32339	IN	denotes	of
T27838	32340-32345	NNP	denotes	siRNA
T27839	32346-32349	CD	denotes	p65
T27840	32349-32350	,	denotes	,
T27841	32351-32353	CC	denotes	or
T27842	32354-32359	NN	denotes	siRNA
T27843	32360-32367	NN	denotes	control
T27844	32368-32369	-LRB-	denotes	(
T27845	32369-32372	CD	denotes	200
T27846	32373-32377	NN	denotes	pmol
T27847	32377-32378	-RRB-	denotes	)
T27848	32378-32379	:	denotes	;
T27849	32380-32382	CD	denotes	48
T27850	32383-32384	NN	denotes	h
T27851	32385-32390	RB	denotes	later
T27852	32390-32391	,	denotes	,
T27853	32392-32396	NNP	denotes	ChIP
T27854	32397-32400	VBD	denotes	was
T27855	32401-32410	VBN	denotes	performed
T27856	32411-32415	IN	denotes	with
T27857	32416-32425	JJ	denotes	anti-FLAG
T27858	32426-32434	NN	denotes	antibody
T27859	32434-32435	.	denotes	.
T27860	32436-32445	JJ	denotes	Real-time
T27861	32446-32449	NNP	denotes	PCR
T27862	32450-32453	VBD	denotes	was
T27863	32454-32463	VBN	denotes	performed
T27864	32464-32468	IN	denotes	with
T27865	32469-32476	NNS	denotes	primers
T27866	32477-32485	JJ	denotes	specific
T27867	32486-32489	IN	denotes	for
T27868	32490-32493	DT	denotes	the
T27869	32494-32503	VBN	denotes	indicated
T27870	32504-32513	NNS	denotes	promoters
T27871	32513-32514	.	denotes	.
T27872	32515-32516	-LRB-	denotes	(
T27873	32516-32517	NNP	denotes	J
T27874	32517-32518	-RRB-	denotes	)
T27875	32519-32523	NNP	denotes	HeLa
T27876	32524-32529	NNS	denotes	cells
T27877	32530-32531	-LRB-	denotes	(
T27878	32531-32532	CD	denotes	5
T27879	32533-32534	CD	denotes	×
T27880	32535-32538	CD	denotes	106
T27881	32538-32539	-RRB-	denotes	)
T27882	32540-32544	VBD	denotes	were
T27883	32545-32556	VBN	denotes	transfected
T27884	32557-32561	IN	denotes	with
T27885	32562-32567	JJ	denotes	empty
T27886	32568-32574	NN	denotes	vector
T27887	32574-32575	,	denotes	,
T27888	32576-32587	NN	denotes	p3xFLAG-Tat
T27889	32588-32589	-LRB-	denotes	(
T27890	32589-32591	CD	denotes	10
T27891	32592-32594	NN	denotes	µg
T27892	32594-32595	-RRB-	denotes	)
T27893	32595-32596	,	denotes	,
T27894	32597-32599	CC	denotes	or
T27895	32600-32605	JJ	denotes	empty
T27896	32606-32612	NN	denotes	vector
T27897	32613-32617	CC	denotes	plus
T27898	32618-32623	NNP	denotes	siRNA
T27899	32624-32629	NNP	denotes	IκB-α
T27900	32630-32632	CC	denotes	or
T27901	32633-32638	NNP	denotes	siRNA
T27902	32639-32646	NN	denotes	control
T27903	32647-32648	-LRB-	denotes	(
T27904	32648-32651	CD	denotes	200
T27905	32652-32656	NN	denotes	pmol
T27906	32656-32657	-RRB-	denotes	)
T27907	32657-32658	:	denotes	;
T27908	32659-32661	CD	denotes	48
T27909	32662-32663	NN	denotes	h
T27910	32664-32669	RB	denotes	later
T27911	32669-32670	,	denotes	,
T27912	32671-32675	NNP	denotes	ChIP
T27913	32676-32679	VBD	denotes	was
T27914	32680-32689	VBN	denotes	performed
T27915	32690-32694	IN	denotes	with
T27916	32695-32705	JJ	denotes	anti-IκB-α
T27917	32706-32714	NN	denotes	antibody
T27918	32714-32715	.	denotes	.
T27919	32716-32725	JJ	denotes	Real-time
T27920	32726-32729	NNP	denotes	PCR
T27921	32730-32733	VBD	denotes	was
T27922	32734-32743	VBN	denotes	performed
T27923	32744-32748	IN	denotes	with
T27924	32749-32756	NNS	denotes	primers
T27925	32757-32765	JJ	denotes	specific
T27926	32766-32769	IN	denotes	for
T27927	32770-32773	DT	denotes	the
T27928	32774-32783	VBN	denotes	indicated
T27929	32784-32793	NNS	denotes	promoters
T27930	32793-32794	.	denotes	.
T27931	32795-32801	NNS	denotes	Values
T27932	32802-32803	-LRB-	denotes	(
T27933	32803-32807	JJ	denotes	mean
T27934	32808-32809	NN	denotes	±
T27935	32810-32812	NNP	denotes	SE
T27936	32812-32813	,	denotes	,
T27937	32814-32815	JJ	denotes	n
T27938	32816-32817	SYM	denotes	=
T27939	32818-32819	LS	denotes	3
T27940	32819-32820	-RRB-	denotes	)
T27941	32821-32824	VBP	denotes	are
T27942	32825-32830	VBN	denotes	shown
T27943	32830-32831	.	denotes	.
T27944	32832-32835	DT	denotes	The
T27945	32836-32845	NNS	denotes	asterisks
T27946	32846-32854	VBP	denotes	indicate
T27947	32855-32868	RB	denotes	statistically
T27948	32869-32880	JJ	denotes	significant
T27949	32881-32892	NNS	denotes	differences
T27950	32893-32901	VBN	denotes	compared
T27951	32902-32904	TO	denotes	to
T27952	32905-32908	DT	denotes	the
T27953	32909-32916	NN	denotes	control
T27954	32917-32918	-LRB-	denotes	(
T27955	32918-32923	JJ	denotes	empty
T27956	32924-32930	NN	denotes	vector
T27957	32930-32931	-RRB-	denotes	)
T27958	32932-32941	VBG	denotes	according
T27959	32942-32944	TO	denotes	to
T27960	32945-32948	DT	denotes	the
T27961	32949-32956	NN	denotes	Student
T27962	32956-32958	POS	denotes	's
T27963	32959-32965	JJS	denotes	t-test
T27964	32966-32967	-LRB-	denotes	(
T27965	32967-32968	NNP	denotes	P
T27966	32969-32970	CD	denotes	<
T27967	32971-32975	CD	denotes	0.05
T27968	32975-32976	-RRB-	denotes	)
T27969	32976-32977	.	denotes	.
T28367	35881-35883	IN	denotes	In
T28368	35884-35889	JJ	denotes	HIV-1
T28369	35890-35899	NN	denotes	infection
T28370	35900-35903	NNP	denotes	Tat
T28371	35904-35912	VBZ	denotes	sustains
T28372	35913-35916	DT	denotes	the
T28373	35917-35922	JJ	denotes	NF-κB
T28374	35923-35931	NN	denotes	activity
T28375	35932-35935	CC	denotes	and
T28376	35936-35944	VBZ	denotes	enhances
T28377	35945-35948	DT	denotes	the
T28378	35949-35955	JJ	denotes	MIP-1α
T28379	35956-35966	NN	denotes	expression
T28380	35967-35970	IN	denotes	via
T28381	35971-35982	NN	denotes	interaction
T28382	35983-35987	IN	denotes	with
T28383	35988-35993	NNP	denotes	IκB-α
T28384	35994-35997	CC	denotes	and
T28385	35998-36001	NNS	denotes	p65
T28386	36001-36002	.	denotes	.
T28387	36003-36007	CD	denotes	U937
T28388	36008-36013	NNS	denotes	cells
T28389	36014-36015	-LRB-	denotes	(
T28390	36015-36016	CD	denotes	5
T28391	36017-36018	RB	denotes	×
T28392	36019-36022	CD	denotes	107
T28393	36022-36023	-RRB-	denotes	)
T28394	36024-36028	VBD	denotes	were
T28395	36029-36040	VBN	denotes	transfected
T28396	36041-36045	IN	denotes	with
T28397	36046-36051	NNP	denotes	siRNA
T28398	36052-36055	NNP	denotes	Tat
T28399	36055-36056	,	denotes	,
T28400	36057-36062	NNP	denotes	siRNA
T28401	36063-36070	NN	denotes	control
T28402	36071-36072	-LRB-	denotes	(
T28403	36072-36073	CD	denotes	2
T28404	36074-36078	NN	denotes	nmol
T28405	36078-36079	-RRB-	denotes	)
T28406	36079-36080	,	denotes	,
T28407	36081-36083	CC	denotes	or
T28408	36084-36088	VBD	denotes	left
T28409	36089-36102	JJ	denotes	untransfected
T28410	36102-36103	:	denotes	;
T28411	36104-36106	CD	denotes	24
T28412	36107-36108	NN	denotes	h
T28413	36109-36114	RB	denotes	later
T28414	36115-36120	NNS	denotes	cells
T28415	36121-36125	VBD	denotes	were
T28416	36126-36134	VBN	denotes	infected
T28417	36135-36139	IN	denotes	with
T28418	36140-36156	JJ	denotes	HXB2-pseudotyped
T28419	36157-36162	NN	denotes	NL4-3
T28420	36162-36163	.	denotes	.
T28421	36163-36171	NN	denotes	FLAG-Tat
T28422	36171-36172	.	denotes	.
T28423	36172-36173	NN	denotes	R
T28424	36173-36174	NN	denotes	−
T28425	36174-36175	NNP	denotes	E
T28426	36175-36176	CD	denotes	−
T28427	36177-36184	NNS	denotes	virions
T28428	36185-36186	-LRB-	denotes	(
T28429	36186-36189	CD	denotes	500
T28430	36190-36192	NN	denotes	ng
T28431	36193-36195	IN	denotes	of
T28432	36196-36199	CD	denotes	p24
T28433	36199-36200	-RRB-	denotes	)
T28434	36201-36204	CC	denotes	and
T28435	36205-36214	VBD	denotes	harvested
T28436	36215-36217	IN	denotes	at
T28437	36218-36221	DT	denotes	the
T28438	36222-36231	JJ	denotes	indicated
T28439	36232-36236	NN	denotes	time
T28440	36236-36237	.	denotes	.
T28441	36238-36239	-LRB-	denotes	(
T28442	36239-36240	NNP	denotes	A
T28443	36240-36241	-RRB-	denotes	)
T28444	36242-36251	JJ	denotes	Real-time
T28445	36252-36255	NNP	denotes	PCR
T28446	36256-36258	IN	denotes	of
T28447	36259-36264	JJ	denotes	total
T28448	36265-36268	NNP	denotes	RNA
T28449	36269-36277	VBD	denotes	measured
T28450	36278-36281	DT	denotes	the
T28451	36282-36285	NNP	denotes	Tat
T28452	36286-36296	NN	denotes	expression
T28453	36296-36297	:	denotes	;
T28454	36298-36303	NNP	denotes	ELISA
T28455	36304-36312	VBD	denotes	measured
T28456	36313-36316	DT	denotes	the
T28457	36317-36323	NN	denotes	amount
T28458	36324-36326	IN	denotes	of
T28459	36327-36330	CD	denotes	p24
T28460	36331-36333	IN	denotes	in
T28461	36334-36339	JJ	denotes	whole
T28462	36340-36344	NN	denotes	cell
T28463	36345-36353	NNS	denotes	extracts
T28464	36353-36354	.	denotes	.
T28465	36355-36356	-LRB-	denotes	(
T28466	36356-36357	NNP	denotes	B
T28467	36357-36358	-RRB-	denotes	)
T28468	36359-36362	DT	denotes	The
T28469	36363-36370	JJ	denotes	binding
T28470	36371-36373	IN	denotes	of
T28471	36374-36377	CD	denotes	p65
T28472	36378-36380	TO	denotes	to
T28473	36381-36384	DT	denotes	the
T28474	36385-36390	NNP	denotes	NF-κB
T28475	36391-36406	JJ	denotes	double-stranded
T28476	36407-36422	NN	denotes	oligonucleotide
T28477	36423-36426	VBD	denotes	was
T28478	36427-36435	VBN	denotes	measured
T28479	36436-36438	IN	denotes	in
T28480	36439-36446	JJ	denotes	nuclear
T28481	36447-36455	NNS	denotes	extracts
T28482	36456-36457	-LRB-	denotes	(
T28483	36457-36459	CD	denotes	10
T28484	36460-36462	NN	denotes	µg
T28485	36462-36463	-RRB-	denotes	)
T28486	36464-36469	VBG	denotes	using
T28487	36470-36473	DT	denotes	the
T28488	36474-36479	NNP	denotes	NF-κB
T28489	36480-36493	NNP	denotes	Transcription
T28490	36494-36500	NNP	denotes	Factor
T28491	36501-36506	NNP	denotes	ELISA
T28492	36507-36512	NN	denotes	assay
T28493	36513-36516	NN	denotes	kit
T28494	36517-36518	-LRB-	denotes	(
T28495	36518-36524	NNP	denotes	Cayman
T28496	36524-36525	-RRB-	denotes	)
T28497	36525-36526	.	denotes	.
T28498	36527-36528	-LRB-	denotes	(
T28499	36528-36529	$	denotes	C
T28500	36529-36530	-RRB-	denotes	)
T28501	36531-36534	DT	denotes	The
T28502	36535-36540	JJ	denotes	IκB-α
T28503	36541-36548	NN	denotes	content
T28504	36549-36552	VBD	denotes	was
T28505	36553-36561	VBN	denotes	analysed
T28506	36562-36564	IN	denotes	by
T28507	36565-36567	CD	denotes	12
T28508	36567-36568	NN	denotes	%
T28509	36569-36572	NNP	denotes	SDS
T28510	36573-36577	NNP	denotes	PAGE
T28511	36578-36581	CC	denotes	and
T28512	36582-36589	JJ	denotes	western
T28513	36590-36598	VBG	denotes	blotting
T28514	36599-36601	IN	denotes	of
T28515	36602-36611	JJ	denotes	cytosolic
T28516	36612-36620	NNS	denotes	extracts
T28517	36621-36622	-LRB-	denotes	(
T28518	36622-36624	CD	denotes	20
T28519	36625-36627	NN	denotes	µg
T28520	36627-36628	-RRB-	denotes	)
T28521	36629-36634	VBG	denotes	using
T28522	36635-36645	JJ	denotes	anti-IκB-α
T28523	36646-36654	NN	denotes	antibody
T28524	36654-36655	.	denotes	.
T28525	36656-36668	NNP	denotes	Densitometry
T28526	36669-36675	NNS	denotes	values
T28527	36676-36677	-LRB-	denotes	(
T28528	36677-36678	NNP	denotes	D
T28529	36678-36679	-RRB-	denotes	)
T28530	36680-36682	IN	denotes	of
T28531	36683-36686	DT	denotes	the
T28532	36687-36692	NNS	denotes	bands
T28533	36693-36697	VBD	denotes	were
T28534	36698-36707	VBN	denotes	expressed
T28535	36708-36710	IN	denotes	as
T28536	36711-36715	VBP	denotes	fold
T28537	36716-36724	NN	denotes	increase
T28538	36725-36730	IN	denotes	above
T28539	36731-36734	DT	denotes	the
T28540	36735-36742	NN	denotes	control
T28541	36743-36744	-LRB-	denotes	(
T28542	36744-36748	JJ	denotes	mock
T28543	36748-36749	-RRB-	denotes	)
T28544	36749-36750	.	denotes	.
T28545	36751-36752	-LRB-	denotes	(
T28546	36752-36753	NNP	denotes	D
T28547	36753-36754	-RRB-	denotes	)
T28548	36755-36758	NNP	denotes	IKK
T28549	36759-36767	NN	denotes	activity
T28550	36768-36771	VBD	denotes	was
T28551	36772-36780	VBN	denotes	measured
T28552	36781-36783	IN	denotes	in
T28553	36784-36793	JJ	denotes	cytosolic
T28554	36794-36798	NN	denotes	cell
T28555	36799-36807	NNS	denotes	extracts
T28556	36808-36809	-LRB-	denotes	(
T28557	36809-36812	CD	denotes	100
T28558	36813-36815	NN	denotes	µg
T28559	36815-36816	-RRB-	denotes	)
T28560	36817-36822	VBG	denotes	using
T28561	36823-36829	NNP	denotes	HTScan
T28562	36830-36833	NNP	denotes	IKK
T28563	36834-36840	NNP	denotes	Kinase
T28564	36841-36846	NNP	denotes	Assay
T28565	36847-36848	-LRB-	denotes	(
T28566	36848-36852	NNP	denotes	Cell
T28567	36853-36862	NNP	denotes	Signaling
T28568	36863-36873	NNP	denotes	Technology
T28569	36873-36874	-RRB-	denotes	)
T28570	36874-36875	.	denotes	.
T28571	36876-36877	-LRB-	denotes	(
T28572	36877-36878	NNP	denotes	E
T28573	36878-36879	-RRB-	denotes	)
T28574	36880-36884	NNP	denotes	U937
T28575	36885-36890	NNS	denotes	cells
T28576	36891-36892	-LRB-	denotes	(
T28577	36892-36893	CD	denotes	5
T28578	36894-36895	RB	denotes	×
T28579	36896-36899	CD	denotes	107
T28580	36899-36900	-RRB-	denotes	)
T28581	36901-36905	VBD	denotes	were
T28582	36906-36914	VBN	denotes	infected
T28583	36915-36919	IN	denotes	with
T28584	36920-36936	JJ	denotes	HXB2-pseudotyped
T28585	36937-36942	NN	denotes	NL4-3
T28586	36942-36943	.	denotes	.
T28587	36943-36951	NN	denotes	FLAG-Tat
T28588	36951-36952	.	denotes	.
T28589	36952-36953	NN	denotes	R
T28590	36953-36954	NN	denotes	−
T28591	36954-36955	NNP	denotes	E
T28592	36955-36956	CD	denotes	−
T28593	36957-36964	NNS	denotes	virions
T28594	36964-36965	,	denotes	,
T28595	36966-36968	CC	denotes	or
T28596	36969-36973	VBD	denotes	left
T28597	36974-36984	JJ	denotes	uninfected
T28598	36984-36985	.	denotes	.
T28599	36986-37002	CD	denotes	Twenty-four-hour
T28600	37003-37017	NN	denotes	post-infection
T28601	37017-37018	,	denotes	,
T28602	37019-37023	NN	denotes	cell
T28603	37024-37032	NNS	denotes	extracts
T28604	37033-37034	-LRB-	denotes	(
T28605	37034-37035	CD	denotes	1
T28606	37036-37038	NN	denotes	mg
T28607	37038-37039	-RRB-	denotes	)
T28608	37040-37044	VBD	denotes	were
T28609	37045-37063	VBN	denotes	immunoprecipitated
T28610	37064-37068	IN	denotes	with
T28611	37069-37076	NN	denotes	protein
T28612	37077-37096	JJ	denotes	G-Sepharose-coupled
T28613	37097-37106	JJ	denotes	anti-FLAG
T28614	37107-37115	NN	denotes	antibody
T28615	37115-37116	.	denotes	.
T28616	37117-37132	NNS	denotes	Immunocomplexes
T28617	37133-37137	VBD	denotes	were
T28618	37138-37147	VBN	denotes	separated
T28619	37148-37150	IN	denotes	by
T28620	37151-37153	CD	denotes	12
T28621	37153-37154	NN	denotes	%
T28622	37155-37158	NNP	denotes	SDS
T28623	37159-37163	NNP	denotes	PAGE
T28624	37164-37167	CC	denotes	and
T28625	37168-37176	VBN	denotes	analysed
T28626	37177-37179	IN	denotes	by
T28627	37180-37187	JJ	denotes	western
T28628	37188-37196	VBG	denotes	blotting
T28629	37197-37201	IN	denotes	with
T28630	37202-37210	JJ	denotes	anti-p65
T28631	37210-37211	,	denotes	,
T28632	37212-37221	JJ	denotes	anti-FLAG
T28633	37222-37225	CC	denotes	and
T28634	37226-37236	JJ	denotes	anti-IκB-α
T28635	37237-37247	NNS	denotes	antibodies
T28636	37247-37248	.	denotes	.
T28637	37249-37250	-LRB-	denotes	(
T28638	37250-37251	NN	denotes	F
T28639	37251-37252	-RRB-	denotes	)
T28640	37253-37257	NNP	denotes	U937
T28641	37258-37263	NNS	denotes	cells
T28642	37264-37265	-LRB-	denotes	(
T28643	37265-37266	CD	denotes	5
T28644	37267-37268	RB	denotes	×
T28645	37269-37272	CD	denotes	107
T28646	37272-37273	-RRB-	denotes	)
T28647	37274-37278	VBD	denotes	were
T28648	37279-37290	VBN	denotes	transfected
T28649	37291-37295	IN	denotes	with
T28650	37296-37301	NNP	denotes	siRNA
T28651	37302-37305	NNP	denotes	Tat
T28652	37305-37306	,	denotes	,
T28653	37307-37312	NNP	denotes	siRNA
T28654	37313-37316	CD	denotes	p65
T28655	37316-37317	,	denotes	,
T28656	37318-37323	NNP	denotes	siRNA
T28657	37324-37331	NN	denotes	control
T28658	37332-37333	-LRB-	denotes	(
T28659	37333-37334	CD	denotes	2
T28660	37335-37339	NN	denotes	nmol
T28661	37339-37340	-RRB-	denotes	)
T28662	37340-37341	,	denotes	,
T28663	37342-37344	CC	denotes	or
T28664	37345-37349	VBD	denotes	left
T28665	37350-37363	JJ	denotes	untransfected
T28666	37363-37364	:	denotes	;
T28667	37365-37367	CD	denotes	24
T28668	37368-37369	NN	denotes	h
T28669	37370-37375	RB	denotes	later
T28670	37375-37376	,	denotes	,
T28671	37377-37382	NNS	denotes	cells
T28672	37383-37387	VBD	denotes	were
T28673	37388-37396	VBN	denotes	infected
T28674	37397-37401	IN	denotes	with
T28675	37402-37418	JJ	denotes	HXB2-pseudotyped
T28676	37419-37424	NN	denotes	NL4-3
T28677	37424-37425	.	denotes	.
T28678	37425-37433	NN	denotes	FLAG-Tat
T28679	37433-37434	.	denotes	.
T28680	37434-37435	NN	denotes	R
T28681	37435-37436	NN	denotes	−
T28682	37436-37437	NNP	denotes	E
T28683	37437-37438	CD	denotes	−
T28684	37439-37446	NNS	denotes	virions
T28685	37446-37447	,	denotes	,
T28686	37448-37450	CC	denotes	or
T28687	37451-37455	VBD	denotes	left
T28688	37456-37466	JJ	denotes	uninfected
T28689	37466-37467	.	denotes	.
T28690	37468-37484	CD	denotes	Twenty-four-hour
T28691	37485-37499	JJ	denotes	post-infection
T28692	37499-37500	,	denotes	,
T28693	37501-37506	JJ	denotes	total
T28694	37507-37510	NNP	denotes	RNA
T28695	37511-37514	VBD	denotes	was
T28696	37515-37523	VBN	denotes	analysed
T28697	37524-37527	IN	denotes	for
T28698	37528-37534	JJ	denotes	MIP-1α
T28699	37535-37545	NN	denotes	expression
T28700	37546-37548	IN	denotes	by
T28701	37549-37558	JJ	denotes	real-time
T28702	37559-37562	NNP	denotes	PCR
T28703	37562-37563	.	denotes	.
T28704	37564-37565	-LRB-	denotes	(
T28705	37565-37566	NNP	denotes	G
T28706	37567-37570	CC	denotes	and
T28707	37571-37572	NNP	denotes	H
T28708	37572-37573	-RRB-	denotes	)
T28709	37574-37578	NNP	denotes	U937
T28710	37579-37584	NNS	denotes	cells
T28711	37585-37586	-LRB-	denotes	(
T28712	37586-37587	CD	denotes	5
T28713	37588-37589	RB	denotes	×
T28714	37590-37593	CD	denotes	107
T28715	37593-37594	-RRB-	denotes	)
T28716	37595-37599	VBD	denotes	were
T28717	37600-37611	VBN	denotes	transfected
T28718	37612-37616	IN	denotes	with
T28719	37617-37622	NNP	denotes	siRNA
T28720	37623-37626	NNP	denotes	Tat
T28721	37627-37629	CC	denotes	or
T28722	37630-37635	NNP	denotes	siRNA
T28723	37636-37643	NN	denotes	control
T28724	37644-37645	-LRB-	denotes	(
T28725	37645-37646	CD	denotes	2
T28726	37647-37651	NN	denotes	nmol
T28727	37651-37652	-RRB-	denotes	)
T28728	37652-37653	,	denotes	,
T28729	37654-37656	CC	denotes	or
T28730	37657-37661	VBD	denotes	left
T28731	37662-37675	JJ	denotes	untransfected
T28732	37675-37676	:	denotes	;
T28733	37677-37679	CD	denotes	24
T28734	37680-37681	NN	denotes	h
T28735	37682-37687	RB	denotes	later
T28736	37687-37688	,	denotes	,
T28737	37689-37694	NNS	denotes	cells
T28738	37695-37699	VBD	denotes	were
T28739	37700-37708	VBN	denotes	infected
T28740	37709-37713	IN	denotes	with
T28741	37714-37730	JJ	denotes	HXB2-pseudotyped
T28742	37731-37736	NN	denotes	NL4-3
T28743	37736-37737	.	denotes	.
T28744	37737-37745	NN	denotes	FLAG-Tat
T28745	37745-37746	.	denotes	.
T28746	37746-37747	NN	denotes	R
T28747	37747-37748	NN	denotes	−
T28748	37748-37749	NNP	denotes	E
T28749	37749-37750	CD	denotes	−
T28750	37751-37758	NNS	denotes	virions
T28751	37758-37759	,	denotes	,
T28752	37760-37762	CC	denotes	or
T28753	37763-37767	VBD	denotes	left
T28754	37768-37778	JJ	denotes	uninfected
T28755	37778-37779	.	denotes	.
T28756	37780-37796	CD	denotes	Twenty-four-hour
T28757	37797-37811	NN	denotes	post-infection
T28758	37811-37812	,	denotes	,
T28759	37813-37817	NNP	denotes	ChIP
T28760	37818-37821	VBD	denotes	was
T28761	37822-37831	VBN	denotes	performed
T28762	37832-37836	IN	denotes	with
T28763	37837-37845	JJ	denotes	anti-p65
T28764	37846-37847	-LRB-	denotes	(
T28765	37847-37848	NNP	denotes	G
T28766	37848-37849	-RRB-	denotes	)
T28767	37850-37852	CC	denotes	or
T28768	37853-37862	JJ	denotes	anti-FLAG
T28769	37863-37864	-LRB-	denotes	(
T28770	37864-37865	NNP	denotes	H
T28771	37865-37866	-RRB-	denotes	)
T28772	37866-37867	.	denotes	.
T28773	37868-37877	JJ	denotes	Real-time
T28774	37878-37881	NNP	denotes	PCR
T28775	37882-37885	VBD	denotes	was
T28776	37886-37895	VBN	denotes	performed
T28777	37896-37900	IN	denotes	with
T28778	37901-37908	NNS	denotes	primers
T28779	37909-37917	JJ	denotes	specific
T28780	37918-37921	IN	denotes	for
T28781	37922-37928	NNP	denotes	MIP-1α
T28782	37929-37932	CC	denotes	and
T28783	37933-37938	NNP	denotes	GAPDH
T28784	37939-37948	NNS	denotes	promoters
T28785	37948-37949	.	denotes	.
T28786	37950-37956	NNS	denotes	Values
T28787	37957-37958	-LRB-	denotes	(
T28788	37958-37962	JJ	denotes	mean
T28789	37963-37964	NN	denotes	±
T28790	37965-37967	NNP	denotes	SE
T28791	37967-37968	,	denotes	,
T28792	37969-37970	JJ	denotes	n
T28793	37971-37972	SYM	denotes	=
T28794	37973-37974	LS	denotes	3
T28795	37974-37975	-RRB-	denotes	)
T28796	37976-37979	VBP	denotes	are
T28797	37980-37985	VBN	denotes	shown
T28798	37985-37986	.	denotes	.
T28799	37987-37990	DT	denotes	The
T28800	37991-38000	NNS	denotes	asterisks
T28801	38001-38009	VBP	denotes	indicate
T28802	38010-38023	RB	denotes	statistically
T28803	38024-38035	JJ	denotes	significant
T28804	38036-38047	NNS	denotes	differences
T28805	38048-38056	VBN	denotes	compared
T28806	38057-38059	TO	denotes	to
T28807	38060-38063	DT	denotes	the
T28808	38064-38071	NN	denotes	control
T28809	38072-38073	-LRB-	denotes	(
T28810	38073-38077	JJ	denotes	mock
T28811	38077-38078	-RRB-	denotes	)
T28812	38078-38079	,	denotes	,
T28813	38080-38089	VBG	denotes	according
T28814	38090-38092	TO	denotes	to
T28815	38093-38096	DT	denotes	the
T28816	38097-38104	NN	denotes	Student
T28817	38104-38106	POS	denotes	's
T28818	38107-38113	JJS	denotes	t-test
T28819	38114-38115	-LRB-	denotes	(
T28820	38115-38116	NNP	denotes	P
T28821	38117-38118	CD	denotes	<
T28822	38119-38123	CD	denotes	0.05
T28823	38123-38124	-RRB-	denotes	)
T28824	38124-38125	.	denotes	.
T29196	43632-43637	NNP	denotes	Model
T29197	43638-43640	IN	denotes	of
T29198	43641-43647	NNP	denotes	MIP-1α
T29199	43648-43663	JJ	denotes	transcriptional
T29200	43664-43674	NN	denotes	activation
T29201	43675-43677	IN	denotes	by
T29202	43678-43683	NNP	denotes	HIV-1
T29203	43684-43687	NNP	denotes	Tat
T29204	43687-43688	.	denotes	.
T29205	43689-43690	-LRB-	denotes	(
T29206	43690-43691	NNP	denotes	A
T29207	43691-43692	-RRB-	denotes	)
T29208	43693-43695	IN	denotes	In
T29209	43696-43703	NN	denotes	absence
T29210	43704-43706	IN	denotes	of
T29211	43707-43710	NNP	denotes	Tat
T29212	43710-43711	,	denotes	,
T29213	43712-43717	NNP	denotes	IκB-α
T29214	43718-43726	VBZ	denotes	occupies
T29215	43727-43730	DT	denotes	the
T29216	43731-43736	JJ	denotes	NF-κB
T29217	43737-43745	NN	denotes	enhancer
T29218	43746-43748	IN	denotes	of
T29219	43749-43752	DT	denotes	the
T29245	43882-43891	VBZ	denotes	activates
T29246	43892-43895	DT	denotes	the
T29247	43896-43902	JJ	denotes	MIP-1α
T29248	43903-43913	NN	denotes	expression
T29249	43914-43916	IN	denotes	by
T29250	43917-43925	VBG	denotes	removing
T29251	43926-43929	DT	denotes	the
T29252	43930-43935	JJ	denotes	IκB-α
T29253	43936-43945	NN	denotes	repressor
T29254	43946-43950	IN	denotes	from
T29255	43951-43954	DT	denotes	the
T29256	43955-43960	NNP	denotes	NF-κB
T29257	43961-43969	NN	denotes	enhancer
T29258	43969-43970	,	denotes	,
T29259	43971-43974	CC	denotes	and
T29260	43975-43977	IN	denotes	by
T29261	43978-43988	VBG	denotes	increasing
T29262	43989-43992	DT	denotes	the
T29263	43993-44000	JJ	denotes	binding
T29264	44001-44003	IN	denotes	of
T29265	44004-44007	CD	denotes	p65
T29266	44008-44013	JJ	denotes	NF-κB
T29267	44014-44021	NN	denotes	complex
T29268	44022-44024	TO	denotes	to
T29269	44025-44028	DT	denotes	the
T29270	44029-44034	JJ	denotes	NF-κB
T29271	44035-44043	NN	denotes	enhancer
T29272	44043-44044	.	denotes	.
T7557	12455-12456	-RRB-	denotes	)
T7558	12457-12459	IN	denotes	at
T7559	12460-12463	DT	denotes	the
T7560	12464-12469	JJ	denotes	final
T7561	12470-12483	NN	denotes	concentration
T7562	12484-12486	IN	denotes	of
T7563	12487-12488	CD	denotes	1
T7564	12488-12489	NN	denotes	%
T7565	12489-12490	.	denotes	.
T7566	12491-12496	IN	denotes	After
T7567	12497-12499	CD	denotes	10
T7568	12500-12503	NN	denotes	min
T7569	12503-12504	,	denotes	,
T7570	12505-12513	JJ	denotes	ice-cold
T7571	12514-12517	NNP	denotes	PBS
T7572	12518-12522	CC	denotes	plus
T7573	12523-12528	CD	denotes	0.125
T7574	12529-12530	NNP	denotes	M
T7575	12531-12538	NN	denotes	glycine
T7576	12539-12542	VBD	denotes	was
T7577	12543-12548	VBN	denotes	added
T7578	12548-12549	,	denotes	,
T7579	12550-12553	CC	denotes	and
T7580	12554-12560	NNS	denotes	plates
T7581	12561-12565	VBD	denotes	were
T7582	12566-12577	VBN	denotes	transferred
T7583	12578-12580	IN	denotes	on
T7584	12581-12584	NN	denotes	ice
T7585	12584-12585	,	denotes	,
T7586	12586-12592	VBD	denotes	washed
T7587	12593-12604	RB	denotes	extensively
T7588	12605-12609	IN	denotes	with
T7880	13889-13891	NNP	denotes	mM
T7881	13892-13896	NNP	denotes	EDTA
T7882	13896-13897	-RRB-	denotes	)
T7883	13897-13898	,	denotes	,
T7884	13899-13902	CC	denotes	and
T7885	13903-13912	VBN	denotes	extracted
T7886	13913-13915	IN	denotes	in
T7887	13916-13918	NNP	denotes	TE
T7888	13919-13925	NN	denotes	buffer
T7889	13926-13936	VBG	denotes	containing
T7890	13937-13938	CD	denotes	2
T7891	13938-13939	NN	denotes	%
T7892	13940-13943	NNP	denotes	SDS
T7893	13943-13944	.	denotes	.
T7894	13945-13952	NN	denotes	Protein
T7895	13953-13956	NNP	denotes	DNA
T7896	13957-13968	NNS	denotes	cross-links
T7897	13969-13973	VBD	denotes	were
T7898	13974-13982	VBN	denotes	reverted
T7899	13983-13985	IN	denotes	by
T7900	13986-13993	NN	denotes	heating
T7901	13994-13996	IN	denotes	at
T7902	13997-13999	CD	denotes	65
T7903	13999-14000	CD	denotes	°
T7904	14000-14001	NNP	denotes	C
T7905	14002-14011	JJ	denotes	overnight
T7906	14011-14012	.	denotes	.
T7907	14013-14016	NNP	denotes	DNA
T7908	14017-14020	VBD	denotes	was
T7909	14021-14028	JJ	denotes	further
T7910	14029-14037	VBN	denotes	purified
T7911	14038-14040	IN	denotes	by
T7912	14041-14049	NNP	denotes	QIAquick
T7913	14050-14053	NNP	denotes	PCR
T7914	14054-14066	NN	denotes	purification
T7945	14243-14250	VBZ	denotes	eluates
T7946	14251-14256	VBG	denotes	using
T7947	14257-14261	NNP	denotes	SYBR
T7948	14262-14269	NNP	denotes	GreenER
T7949	14270-14276	NNP	denotes	Master
T7950	14277-14280	NNP	denotes	Mix
T7951	14281-14282	-LRB-	denotes	(
T7952	14282-14292	NNP	denotes	Invitrogen
T7953	14292-14293	-RRB-	denotes	)
T7954	14293-14294	.	denotes	.
T7955	14295-14304	NNS	denotes	Reactions
T21629	41779-41782	NNS	denotes	p65
T21630	41783-41787	IN	denotes	from
T21631	41788-41791	DT	denotes	the
T21632	41792-41797	JJ	denotes	IκB-α
T21633	41798-41808	NN	denotes	inhibition
T21634	41808-41809	.	denotes	.
T21635	41810-41820	RB	denotes	Altogether
T21636	41821-41826	DT	denotes	these
T21637	41827-41834	NNS	denotes	results
T21638	41835-41847	VBD	denotes	demonstrated
T21639	41848-41852	IN	denotes	that
T21640	41853-41856	NNP	denotes	Tat
T21641	41857-41866	VBD	denotes	abolished
T21642	41867-41870	DT	denotes	the
T21643	41871-41879	JJ	denotes	negative
T21644	41880-41888	NN	denotes	feedback
T21645	41889-41899	NN	denotes	regulation
T21646	41900-41902	IN	denotes	of
T21647	41903-41908	NN	denotes	NF-κB
T21648	41909-41911	IN	denotes	by
T21649	41912-41921	VBG	denotes	hijacking
T21650	41922-41925	DT	denotes	the
T21651	41926-41931	JJ	denotes	IκB-α
T21652	41932-41941	NN	denotes	repressor
T21653	41941-41942	,	denotes	,
T21654	41943-41952	VBG	denotes	shielding
T21655	41953-41956	NNS	denotes	p65
T21656	41957-41961	IN	denotes	from
T21657	41962-41965	DT	denotes	the
T21658	41966-41971	JJ	denotes	IκB-α
T21659	41972-41979	VBP	denotes	embrace
T21660	41979-41980	,	denotes	,
T21661	41981-41984	CC	denotes	and
T21662	41985-41994	VBG	denotes	enforcing
T21663	41995-41998	DT	denotes	the
T21664	41999-42002	CD	denotes	p65
T21665	42003-42010	JJ	denotes	binding
T21666	42011-42013	TO	denotes	to
T21667	42014-42017	NNP	denotes	DNA
T21668	42017-42018	.	denotes	.
T21669	42019-42026	JJ	denotes	Further
T21670	42027-42035	NN	denotes	evidence
T21671	42036-42038	IN	denotes	of
T21672	42039-42042	NNP	denotes	Tat
T24106	16873-16876	CC	denotes	and
T24107	16877-16887	NNP	denotes	Hexokinase
T24108	16888-16890	NNP	denotes	II
T24109	16891-16895	VBD	denotes	were
T24110	16896-16904	VBN	denotes	detected
T24111	16905-16909	IN	denotes	with
T24112	16910-16918	JJ	denotes	specific
T24113	16919-16929	NNS	denotes	antibodies
T29220	43753-43759	JJ	denotes	MIP-1α
T29221	43760-43768	NN	denotes	promoter
T29222	43769-43772	CC	denotes	and
T29223	43773-43779	JJ	denotes	likely
T29224	43780-43789	NNS	denotes	interacts
T29225	43790-43794	IN	denotes	with
T29226	43795-43810	JJ	denotes	transcriptional
T29227	43811-43821	NNS	denotes	repressors
T29228	43821-43822	,	denotes	,
T29229	43823-43827	JJ	denotes	such
T29230	43828-43830	IN	denotes	as
T29231	43831-43836	NNS	denotes	HDACs
T29232	43837-43838	-LRB-	denotes	(
T29233	43838-43840	CD	denotes	65
T29234	43840-43841	-RRB-	denotes	)
T29235	43841-43842	,	denotes	,
T29236	43843-43845	TO	denotes	to
T29237	43846-43853	VB	denotes	inhibit
T29238	43854-43858	NN	denotes	gene
T29239	43859-43872	NN	denotes	transcription
T29240	43872-43873	.	denotes	.
T29241	43874-43875	-LRB-	denotes	(
T29242	43875-43876	NNP	denotes	B
T29243	43876-43877	-RRB-	denotes	)
T29244	43878-43881	NNP	denotes	Tat
R293	T402	T403	amod	Human,immunodeficiency
R294	T403	T405	compound	immunodeficiency,Tat
R295	T404	T405	compound	virus-1,Tat
R296	T405	T406	nsubj	Tat,activates
R297	T406	T422	ccomp	activates,is
R298	T407	T406	dobj	NF-κB,activates
R299	T408	T406	prep	via,activates
R300	T409	T410	amod	physical,interaction
R301	T410	T408	pobj	interaction,via
R302	T411	T410	prep	with,interaction
R303	T412	T411	pobj	IκB-α,with
R304	T413	T412	cc	and,IκB-α
R305	T414	T416	nummod	p65,factor
R306	T415	T416	amod	Nuclear,factor
R307	T416	T410	conj	factor,interaction
R308	T417	T410	punct	(,interaction
R309	T418	T406	npadvmod	NF,activates
R310	T419	T418	punct	),NF
R311	T420	T422	punct	-,is
R312	T421	T422	nsubj	κB,is
R313	T422	T422	ROOT	is,is
R314	T423	T425	det	a,regulator
R315	T424	T425	compound	master,regulator
R316	T425	T422	attr	regulator,is
R317	T426	T425	prep	of,regulator
R318	T427	T428	amod	pro-inflammatory,genes
R319	T428	T426	pobj	genes,of
R320	T429	T422	cc	and,is
R321	T430	T431	auxpass	is,upregulated
R322	T431	T422	conj	upregulated,is
R323	T432	T431	prep	in,upregulated
R324	T433	T434	amod	human,immunodeficiency
R325	T434	T435	compound	immunodeficiency,virus
R326	T435	T432	pobj	virus,in
R327	T436	T435	nummod	1,virus
R328	T437	T440	punct	(,infection
R329	T438	T440	nmod	HIV-1,infection
R330	T439	T440	punct	),infection
R331	T440	T435	appos	infection,virus
R332	T441	T422	punct	.,is
R333	T442	T449	nsubj	Mechanisms,are
R334	T443	T442	acl	underlying,Mechanisms
R335	T444	T446	det	the,deregulation
R336	T445	T446	amod	NF-κB,deregulation
R337	T446	T443	dobj	deregulation,underlying
R338	T447	T446	prep	by,deregulation
R339	T448	T447	pobj	HIV-1,by
R340	T449	T449	ROOT	are,are
R341	T450	T449	acomp	relevant,are
R342	T451	T450	prep	for,relevant
R343	T452	T453	amod	immune,dysfunction
R344	T453	T451	pobj	dysfunction,for
R345	T454	T453	prep	in,dysfunction
R346	T455	T454	pobj	AIDS,in
R347	T456	T449	punct	.,are
R348	T457	T458	nsubj	We,report
R349	T458	T458	ROOT	report,report
R350	T459	T475	mark	that,activated
R351	T460	T475	prep	in,activated
R352	T461	T464	amod	single,infection
R353	T462	T464	compound	round,infection
R354	T463	T464	compound	HIV-1,infection
R355	T464	T460	pobj	infection,in
R356	T465	T460	punct	",",in
R357	T466	T460	cc	or,in
R358	T467	T469	amod	single-pulse,stimulation
R359	T468	T469	compound	PMA,stimulation
R360	T469	T475	dep	stimulation,activated
R361	T470	T475	punct	",",activated
R362	T471	T474	det	the,transactivator
R363	T472	T474	compound	HIV-1,transactivator
R364	T473	T474	compound	Tat,transactivator
R365	T474	T475	nsubj	transactivator,activated
R366	T475	T458	ccomp	activated,report
R367	T476	T475	dobj	NF-κB,activated
R368	T477	T475	prep	by,activated
R369	T478	T477	pcomp	hijacking,by
R370	T479	T481	det	the,IκB-α
R371	T480	T481	compound	inhibitor,IκB-α
R372	T481	T478	dobj	IκB-α,hijacking
R373	T482	T477	cc	and,by
R374	T483	T477	conj	by,by
R375	T484	T483	pcomp	preventing,by
R376	T485	T486	det	the,repressor
R377	T486	T484	dobj	repressor,preventing
R378	T487	T484	oprd	binding,preventing
R379	T488	T487	prep	to,binding
R380	T489	T491	det	the,complex
R381	T490	T491	amod	NF-κB,complex
R382	T491	T488	pobj	complex,to
R383	T492	T458	punct	.,report
R384	T493	T496	advmod	Moreover,associated
R385	T494	T496	punct	",",associated
R386	T495	T496	npadvmod	Tat,associated
R387	T496	T496	ROOT	associated,associated
R388	T497	T496	prep	with,associated
R389	T498	T500	det	the,subunit
R390	T499	T500	nummod	p65,subunit
R391	T500	T497	pobj	subunit,with
R392	T501	T500	prep	of,subunit
R393	T502	T501	pobj	NF-κB,of
R394	T503	T496	cc	and,associated
R395	T504	T496	conj	increased,associated
R396	T505	T508	det	the,affinity
R397	T506	T508	nummod	p65,affinity
R398	T507	T508	amod	DNA-binding,affinity
R399	T508	T504	dobj	affinity,increased
R400	T509	T508	cc	and,affinity
R401	T510	T511	compound	transcriptional,activity
R402	T511	T508	conj	activity,affinity
R403	T512	T496	punct	.,associated
R404	T513	T518	det	The,domains
R405	T514	T518	nmod	arginine,domains
R406	T515	T514	punct	-,arginine
R407	T516	T514	cc	and,arginine
R408	T517	T518	amod	cysteine-rich,domains
R409	T518	T522	nsubjpass	domains,required
R410	T519	T518	prep	of,domains
R411	T520	T519	pobj	Tat,of
R412	T521	T522	auxpass	were,required
R413	T522	T522	ROOT	required,required
R414	T523	T522	prep	for,required
R415	T524	T523	pobj	IκB-α,for
R416	T525	T524	cc	and,IκB-α
R417	T526	T527	nummod	p65,association
R418	T527	T524	conj	association,IκB-α
R419	T528	T527	punct	",",association
R420	T529	T527	advmod	respectively,association
R421	T530	T527	punct	",",association
R422	T531	T523	cc	and,for
R423	T532	T523	conj	for,for
R424	T533	T532	pcomp	sustaining,for
R425	T534	T536	det	the,activity
R426	T535	T536	compound	NF-κB,activity
R427	T536	T533	dobj	activity,sustaining
R428	T537	T522	punct	.,required
R429	T538	T547	prep	Among,activated
R430	T539	T540	det	an,array
R431	T540	T538	pobj	array,Among
R432	T541	T540	prep	of,array
R433	T542	T543	amod	NF-κB-responsive,genes
R434	T543	T541	pobj	genes,of
R435	T544	T547	punct	",",activated
R436	T545	T547	nsubj	Tat,activated
R437	T546	T547	advmod	mostly,activated
R438	T547	T547	ROOT	activated,activated
R439	T548	T550	det	the,expression
R440	T549	T550	amod	MIP-1α,expression
R441	T550	T547	dobj	expression,activated
R442	T551	T547	prep	in,activated
R443	T552	T554	det	a,manner
R444	T553	T554	amod	p65-dependent,manner
R445	T554	T551	pobj	manner,in
R446	T555	T547	punct	",",activated
R447	T556	T547	cc	and,activated
R448	T557	T547	conj	bound,activated
R449	T558	T557	prep	to,bound
R450	T559	T562	det	the,enhancer
R451	T560	T562	compound	MIP-1α,enhancer
R452	T561	T562	compound	NF-κB,enhancer
R453	T562	T558	pobj	enhancer,to
R454	T563	T564	advmod	thus,promoting
R455	T564	T557	conj	promoting,bound
R456	T565	T566	det	the,recruitment
R465	T574	T575	amod	similar,findings
R466	T575	T577	nsubjpass	findings,obtained
R467	T576	T577	auxpass	were,obtained
R468	T577	T547	conj	obtained,activated
R469	T578	T577	prep	for,obtained
R470	T579	T581	det	the,genes
R471	T580	T581	amod	NF-κB-responsive,genes
R472	T581	T578	pobj	genes,for
R473	T582	T581	appos	CSF3,genes
R474	T583	T582	punct	",",CSF3
R475	T584	T582	conj	LTA,CSF3
R476	T585	T584	punct	",",LTA
R477	T586	T584	conj	NFKBIA,LTA
R478	T587	T586	cc	and,NFKBIA
R479	T588	T586	conj	TLR2,NFKBIA
R480	T589	T577	punct	.,obtained
R481	T590	T591	poss	Our,results
R482	T591	T592	nsubj	results,support
R483	T592	T592	ROOT	support,support
R484	T593	T595	det	a,mechanism
R485	T594	T595	amod	novel,mechanism
R486	T595	T592	dobj	mechanism,support
R487	T596	T595	prep	of,mechanism
R488	T597	T598	amod	NF-κB,activation
R489	T598	T596	pobj	activation,of
R490	T599	T592	prep	via,support
R491	T600	T601	amod	physical,interaction
R492	T601	T599	pobj	interaction,via
R493	T602	T601	prep	of,interaction
R494	T603	T602	pobj	Tat,of
R495	T604	T601	prep	with,interaction
R496	T605	T604	pobj	IκB-α,with
R497	T606	T605	cc	and,IκB-α
R498	T607	T605	conj	p65,IκB-α
R499	T608	T592	punct	",",support
R500	T609	T592	cc	and,support
R501	T610	T611	aux	may,contribute
R502	T611	T592	conj	contribute,support
R503	T612	T611	prep	to,contribute
R504	T613	T614	amod	further,insights
R505	T614	T612	pobj	insights,to
R506	T615	T614	prep	into,insights
R507	T616	T617	det	the,deregulation
R508	T617	T615	pobj	deregulation,into
R509	T618	T617	prep	of,deregulation
R510	T619	T621	det	the,response
R511	T620	T621	amod	inflammatory,response
R512	T621	T618	pobj	response,of
R513	T622	T621	prep	by,response
R514	T623	T622	pobj	HIV-1,by
R515	T624	T592	punct	.,support
R2065	T2329	T2330	compound	Nuclear,factor
R2066	T2330	T2337	nmod	factor,factors
R2067	T2331	T2332	punct	(,NF
R2068	T2332	T2330	appos	NF,factor
R2069	T2333	T2332	punct	),NF
R2070	T2334	T2335	punct	-,κB
R2071	T2335	T2337	amod	κB,factors
R2072	T2336	T2337	compound	transcription,factors
R2073	T2337	T2338	nsubj	factors,regulate
R2074	T2338	T2338	ROOT	regulate,regulate
R2075	T2339	T2340	det	the,transcription
R2076	T2340	T2338	dobj	transcription,regulate
R2077	T2341	T2340	prep	of,transcription
R2078	T2342	T2341	pobj	genes,of
R2079	T2343	T2345	nsubjpass	that,involved
R2080	T2344	T2345	auxpass	are,involved
R2081	T2377	T2378	compound	homology,domain
R2082	T2345	T2342	relcl	involved,genes
R2083	T2346	T2345	prep	in,involved
R2084	T2378	T2370	dobj	domain,share
R2085	T2347	T2351	det	the,response
R2086	T2348	T2351	amod	immune,response
R2087	T2379	T2380	punct	(,RHD
R2088	T2380	T2378	appos	RHD,domain
R2089	T2349	T2348	cc	and,immune
R2090	T2381	T2360	punct	),RelA/p65
R2091	T2350	T2348	conj	inflammatory,immune
R2092	T2351	T2346	pobj	response,in
R2093	T2352	T2353	punct	(,1
R2094	T2353	T2342	appos	1,genes
R2095	T2382	T2359	prep	for,includes
R2096	T2354	T2353	punct	),1
R2097	T2355	T2338	punct	.,regulate
R2098	T2356	T2358	det	The,family
R2099	T2357	T2358	compound	NF-κB,family
R2100	T2358	T2359	nsubj	family,includes
R2101	T2359	T2359	ROOT	includes,includes
R2102	T2383	T2382	pobj	homo,for
R2103	T2360	T2359	dobj	RelA/p65,includes
R2104	T2361	T2360	punct	",",RelA/p65
R2105	T2362	T2360	conj	c-Rel,RelA/p65
R2106	T2363	T2362	punct	",",c-Rel
R2107	T2384	T2383	punct	-,homo
R2108	T2364	T2362	conj	RelB,c-Rel
R2109	T2365	T2364	punct	",",RelB
R2110	T2366	T2364	conj	p50,RelB
R2111	T2385	T2383	cc	or,homo
R2112	T2367	T2366	cc	and,p50
R2113	T2368	T2366	conj	p52,p50
R2114	T2369	T2370	nsubj	that,share
R2115	T2370	T2368	relcl	share,p52
R2116	T2386	T2383	conj	hetero-dimerization,homo
R2117	T2371	T2378	det	a,domain
R2118	T2372	T2373	advmod	highly,conserved
R2119	T2373	T2378	amod	conserved,domain
R2120	T2374	T2375	amod	300-amino,acid
R2121	T2387	T2386	cc	and,hetero-dimerization
R2122	T2375	T2378	compound	acid,domain
R2123	T2376	T2378	compound	Rel,domain
R2124	T2388	T2386	conj	DNA-binding,hetero-dimerization
R2125	T2389	T2359	punct	.,includes
R2126	T2390	T2392	det	The,activity
R2127	T2391	T2392	amod	transcriptional,activity
R2128	T2392	T2397	nsubj	activity,depends
R2129	T2474	T2471	relcl	releases,degradation
R2130	T2475	T2478	det	the,complex
R2131	T2476	T2478	amod	functional,complex
R2132	T2393	T2392	prep	of,activity
R2133	T2477	T2478	compound	NF-κB,complex
R2134	T2478	T2474	dobj	complex,releases
R2135	T2394	T2397	det	the,depends
R2136	T2479	T2478	prep	in,complex
R2137	T2480	T2481	det	the,nucleus
R2138	T2481	T2479	pobj	nucleus,in
R2139	T2395	T2397	nsubj	NF-κB,depends
R2140	T2482	T2457	punct	.,phosphorylates
R2141	T2483	T2496	nsubjpass	IκB-α,phosphorylated
R2142	T2484	T2483	punct	",",IκB-α
R2143	T2396	T2397	nsubj	complex,depends
R2144	T2485	T2488	det	the,inhibitor
R2145	T2486	T2487	advmod	most,abundant
R2146	T2397	T2397	ROOT	depends,depends
R2147	T2487	T2488	amod	abundant,inhibitor
R2148	T2488	T2483	appos	inhibitor,IκB-α
R2149	T2489	T2488	prep	of,inhibitor
R2150	T2490	T2489	pobj	NF-κB,of
R2151	T2491	T2492	punct	(,4
R2152	T2398	T2397	prep	on,depends
R2153	T2492	T2490	appos	4,NF-κB
R2154	T2399	T2400	amod	dimer,composition
R2155	T2493	T2492	punct	),4
R2156	T2494	T2496	punct	",",phosphorylated
R2157	T2400	T2398	pobj	composition,on
R2158	T2495	T2496	auxpass	is,phosphorylated
R2159	T2496	T2496	ROOT	phosphorylated,phosphorylated
R2160	T2497	T2496	agent	by,phosphorylated
R2161	T2401	T2407	mark	since,are
R2162	T2498	T2497	pobj	IKK,by
R2163	T2499	T2496	prep	at,phosphorylated
R2164	T2402	T2406	amod	C-terminal,domains
R2165	T2500	T2499	pobj	Ser32,at
R2166	T2501	T2500	cc	and,Ser32
R2167	T2502	T2500	conj	Ser36,Ser32
R2168	T2403	T2406	amod	unrelated,domains
R2169	T2503	T2504	punct	(,5
R2170	T2504	T2502	parataxis	5,Ser36
R2171	T2404	T2406	amod	transcriptional,domains
R2172	T2405	T2406	compound	activation,domains
R2173	T2505	T2504	punct	),5
R2174	T2506	T2502	punct	",",Ser36
R2175	T2406	T2407	nsubj	domains,are
R2176	T2507	T2502	cc	and,Ser36
R2177	T2508	T2509	advmod	subsequently,ubiquitylated
R2178	T2509	T2509	ROOT	ubiquitylated,ubiquitylated
R2179	T2407	T2397	advcl	are,depends
R2180	T2510	T2509	prep	at,ubiquitylated
R2181	T2511	T2510	pobj	Lys21,at
R2182	T2512	T2511	cc	and,Lys21
R2183	T2408	T2407	acomp	present,are
R2184	T2513	T2511	conj	Lys22,Lys21
R2185	T2514	T2516	aux	to,degraded
R2186	T2515	T2516	auxpass	be,degraded
R2187	T2409	T2408	advmod	exclusively,present
R2188	T2516	T2509	xcomp	degraded,ubiquitylated
R2189	T2517	T2516	agent	by,degraded
R2190	T2518	T2520	det	the,proteasome
R2191	T2410	T2407	prep	in,are
R2192	T2519	T2520	amod	26S,proteasome
R2193	T2520	T2517	pobj	proteasome,by
R2194	T2521	T2522	punct	(,6
R2195	T2411	T2410	pobj	p65,in
R2196	T2522	T2520	appos	6,proteasome
R2198	T2523	T2520	punct	),proteasome
R2199	T2524	T2496	punct	.,phosphorylated
R2200	T2525	T2527	det	The,activity
R2201	T2526	T2527	amod	NF-κB,activity
R2202	T2527	T2529	nsubjpass	activity,enhanced
R2203	T2528	T2529	auxpass	is,enhanced
R2204	T2529	T2529	ROOT	enhanced,enhanced
R2205	T2530	T2529	agent	by,enhanced
R2206	T2531	T2530	pobj	phosphorylation,by
R2207	T2532	T2531	prep	of,phosphorylation
R2208	T2533	T2532	pobj	p65,of
R2210	T2534	T2529	prep	at,enhanced
R2211	T2535	T2534	pobj	Ser276,at
R2212	T2536	T2529	agent	by,enhanced
R2213	T2537	T2536	pobj	PKA,by
R2215	T2538	T2537	cc	and,PKA
R2216	T2539	T2537	conj	MSK1,PKA
R2217	T2540	T2539	punct	(,MSK1
R2219	T2541	T2539	parataxis	"7,8",MSK1
R2220	T2542	T2539	punct	),MSK1
R2221	T2543	T2537	punct	",",PKA
R2223	T2544	T2537	conj	Ser311,PKA
R2224	T2545	T2529	agent	by,enhanced
R2225	T2546	T2545	pobj	PKCζ,by
R2227	T2547	T2548	punct	(,9
R2228	T2548	T2546	parataxis	9,PKCζ
R2229	T2549	T2548	punct	),9
R2231	T2550	T2546	cc	and,PKCζ
R2232	T2551	T2546	conj	Ser536,PKCζ
R2233	T2552	T2529	agent	by,enhanced
R2234	T2553	T2552	pobj	IKKα,by
R2236	T2554	T2555	punct	(,"10,11"
R2237	T2555	T2553	parataxis	"10,11",IKKα
R2238	T2556	T2529	punct	),enhanced
R2240	T2557	T2529	punct	.,enhanced
R2241	T2558	T2565	nsubj	Acetylation,increases
R2242	T2559	T2558	prep	of,Acetylation
R2243	T2560	T2559	pobj	p65,of
R2245	T2561	T2558	prep	at,Acetylation
R2246	T2562	T2561	pobj	Lys218,at
R2248	T2563	T2562	cc	and,Lys218
R2249	T2564	T2562	conj	Lys221,Lys218
R2250	T2565	T2565	ROOT	increases,increases
R2251	T2566	T2567	det	the,DNA
R2252	T2567	T2565	dobj	DNA,increases
R2253	T2568	T2565	advcl	binding,increases
R2255	T2569	T2565	cc	and,increases
R2256	T2570	T2565	conj	impairs,increases
R2260	T2427	T2426	prep	with,associate
R2261	T2571	T2572	det	the,association
R2262	T2572	T2570	dobj	association,impairs
R2263	T2573	T2572	prep	with,association
R2264	T2574	T2573	pobj	IκB-α,with
R2265	T2428	T2430	det	the,complex
R2266	T2575	T2574	punct	",",IκB-α
R2267	T2576	T2572	cc	and,association
R2268	T2577	T2572	conj	acetylation,association
R2269	T2429	T2430	compound	NF-κB,complex
R2270	T2578	T2570	prep	at,impairs
R2271	T2579	T2578	pobj	Lys310,at
R2272	T2430	T2427	pobj	complex,with
R2273	T2580	T2565	conj	enhances,increases
R2274	T2581	T2584	det	the,activity
R2275	T2582	T2584	nummod	p65,activity
R2276	T2431	T2420	cc	and,Inhibitors
R2277	T2583	T2584	amod	transcriptional,activity
R2278	T2584	T2580	dobj	activity,enhances
R2279	T2585	T2584	punct	(,activity
R2280	T2432	T2432	ROOT	interfere,interfere
R2281	T2586	T2584	appos	"12,13",activity
R2282	T2587	T2584	punct	),activity
R2283	T2588	T2565	punct	.,increases
R2284	T2433	T2432	prep	with,interfere
R2285	T2589	T2590	nsubj	Post-activation,turn
R2286	T2590	T2595	nsubjpass	turn,regulated
R2287	T2591	T2590	prt	off,turn
R2288	T2434	T2435	poss	its,binding
R2289	T2592	T2591	prep	of,off
R2290	T2593	T2592	pobj	NF-κB,of
R2291	T2594	T2595	auxpass	is,regulated
R2292	T2435	T2433	pobj	binding,with
R2293	T2595	T2595	ROOT	regulated,regulated
R2294	T2596	T2595	agent	by,regulated
R2295	T2597	T2599	amod	negative,loop
R2296	T2436	T2435	prep	to,binding
R2297	T2598	T2599	compound	feedback,loop
R2298	T2599	T2596	pobj	loop,by
R2299	T2600	T2595	prep	through,regulated
R2300	T2601	T2600	pobj	inhibitors,through
R2301	T2602	T2601	prep	under,inhibitors
R2302	T2603	T2605	det	the,control
R2303	T2437	T2436	pobj	DNA,to
R2304	T2604	T2605	amod	transcriptional,control
R2305	T2605	T2602	pobj	control,under
R2306	T2606	T2605	prep	of,control
R2307	T2607	T2606	pobj	NF-κB,of
R2308	T2438	T2439	punct	(,3
R2309	T2608	T2601	punct	",",inhibitors
R2310	T2609	T2610	amod	such,as
R2311	T2610	T2601	prep	as,inhibitors
R2312	T2439	T2437	appos	3,DNA
R2313	T2440	T2439	punct	),3
R2314	T2611	T2615	amod	IκB-α,A20
R2315	T2441	T2432	punct	.,interfere
R2316	T2612	T2611	cc	and,IκB-α
R2317	T2613	T2611	conj	ubiquitin-editing,IκB-α
R2318	T2614	T2615	compound	protein,A20
R2319	T2442	T2457	prep	In,phosphorylates
R2320	T2615	T2610	pobj	A20,as
R2321	T2616	T2615	punct	(,A20
R2322	T2617	T2615	appos	14,A20
R2323	T2443	T2445	det	the,pathway
R2324	T2618	T2617	prep	20,14
R2325	T2619	T2617	punct	),14
R2326	T2620	T2595	punct	.,regulated
R2327	T2444	T2445	amod	canonical,pathway
R2328	T2621	T2638	nsubj	Deacetylation,down-regulate
R2329	T2622	T2621	prep	of,Deacetylation
R2330	T2623	T2622	pobj	p65,of
R2331	T2445	T2442	pobj	pathway,In
R2332	T2624	T2621	prep	by,Deacetylation
R2333	T2625	T2624	pobj	histone,by
R2334	T2626	T2621	conj	deacetylase-3,Deacetylation
R2335	T2446	T2445	prep	of,pathway
R2336	T2627	T2626	cc	or,deacetylase-3
R2337	T2628	T2626	conj	SIRT1,deacetylase-3
R2338	T2629	T2628	punct	",",SIRT1
R2339	T2447	T2448	amod	NF-κB,activation
R2340	T2630	T2628	cc	or,SIRT1
R2341	T2631	T2628	conj	acetylation,SIRT1
R2342	T2632	T2631	prep	of,acetylation
R2343	T2633	T2632	pobj	p65,of
R2344	T2448	T2446	pobj	activation,of
R2345	T2634	T2631	prep	at,acetylation
R2346	T2635	T2634	pobj	Lys122,at
R2347	T2636	T2635	cc	and,Lys122
R2348	T2449	T2457	punct	",",phosphorylates
R2349	T2637	T2635	conj	Lys123,Lys122
R2350	T2638	T2638	ROOT	down-regulate,down-regulate
R2351	T2639	T2641	det	the,activity
R2352	T2640	T2641	compound	NF-κB,activity
R2353	T2641	T2638	dobj	activity,down-regulate
R2354	T2450	T2453	det	the,kinase
R2355	T2642	T2641	punct	(,activity
R2356	T2643	T2641	appos	"12,21,22",activity
R2357	T2644	T2641	punct	),activity
R2358	T2451	T2453	amod	activated,kinase
R2359	T2645	T2638	punct	.,down-regulate
R2360	T2646	T2647	amod	Persistent,activation
R2361	T2647	T2650	nsubj	activation,occurs
R2362	T2452	T2453	compound	IκB,kinase
R2363	T2648	T2647	prep	of,activation
R2364	T2649	T2648	pobj	NF-κB,of
R2365	T2453	T2457	nsubj	kinase,phosphorylates
R2366	T2650	T2650	ROOT	occurs,occurs
R2367	T2454	T2455	punct	(,IKK
R2368	T2651	T2650	prep	in,occurs
R2369	T2652	T2653	amod	human,immunodeficiency
R2370	T2653	T2651	pobj	immunodeficiency,in
R2371	T2455	T2453	appos	IKK,kinase
R2372	T2654	T2656	amod	virus-1,HIV-1
R2373	T2655	T2656	punct	(,HIV-1
R2374	T2656	T2650	npadvmod	HIV-1,occurs
R2375	T2456	T2453	punct	),kinase
R2376	T2657	T2656	punct	),HIV-1
R2377	T2658	T2659	punct	-,infected
R2378	T2659	T2660	amod	infected,monocytes
R2379	T2457	T2457	ROOT	phosphorylates,phosphorylates
R2380	T2660	T2650	conj	monocytes,occurs
R2381	T2661	T2660	punct	",",monocytes
R2382	T2662	T2660	conj	macrophages,monocytes
R2383	T2458	T2457	dobj	IκB,phosphorylates
R2384	T2663	T2662	cc	and,macrophages
R2385	T2664	T2662	conj	microglia,macrophages
R2386	T2665	T2650	punct	",",occurs
R2387	T2459	T2457	prep	at,phosphorylates
R2388	T2666	T2650	cc	and,occurs
R2389	T2667	T2650	conj	enhances,occurs
R2390	T2460	T2462	amod	specific,residues
R2391	T2461	T2462	compound	serine,residues
R2392	T2462	T2459	pobj	residues,at
R2393	T2668	T2669	det	the,expression
R2394	T2463	T2464	nsubj	that,target
R2395	T2669	T2667	dobj	expression,enhances
R2396	T2670	T2669	prep	of,expression
R2397	T2671	T2672	amod	NF-κB-responsive,genes
R2398	T2464	T2462	relcl	target,residues
R2399	T2672	T2670	pobj	genes,of
R2400	T2673	T2672	punct	",",genes
R2401	T2674	T2672	prep	including,genes
R2402	T2675	T2676	amod	pro-inflammatory,cytokines
R2403	T2676	T2674	pobj	cytokines,including
R2404	T2677	T2676	punct	",",cytokines
R2405	T2465	T2466	det	the,protein
R2406	T2678	T2679	compound	cell,adhesion
R2407	T2679	T2680	compound	adhesion,molecules
R2408	T2680	T2676	conj	molecules,cytokines
R2409	T2681	T2680	cc	and,molecules
R2410	T2466	T2464	dobj	protein,target
R2411	T2682	T2680	conj	chemokines,molecules
R2412	T2683	T2682	punct	(,chemokines
R2413	T2467	T2468	aux	to,ubiquitination
R2414	T2684	T2685	nummod	23,25
R2415	T2685	T2682	appos	25,chemokines
R2416	T2686	T2682	punct	),chemokines
R2417	T2468	T2464	advcl	ubiquitination,target
R2418	T2687	T2650	punct	.,occurs
R2419	T2688	T2689	amod	Chronic,inflammation
R2420	T2689	T2690	nsubj	inflammation,is
R2421	T2469	T2468	cc	and,ubiquitination
R2422	T2690	T2690	ROOT	is,is
R2423	T2691	T2693	det	a,cause
R2424	T2692	T2693	amod	major,cause
R2425	T2470	T2471	amod	proteasomal,degradation
R2426	T2693	T2690	attr	cause,is
R2427	T2694	T2693	prep	of,cause
R2428	T2695	T2698	amod	immune,dysfunction
R2429	T2471	T2468	conj	degradation,ubiquitination
R2430	T2696	T2695	cc	and,immune
R2431	T2697	T2695	conj	neuron,immune
R2432	T2698	T2694	pobj	dysfunction,of
R2433	T2472	T2471	punct	",",degradation
R2434	T2699	T2698	prep	in,dysfunction
R2435	T2700	T2699	pobj	AIDS,in
R2436	T2701	T2702	punct	(,"26,27"
R2437	T2473	T2474	nsubj	which,releases
R2438	T2702	T2698	appos	"26,27",dysfunction
R2439	T2703	T2690	punct	),is
R2440	T2765	T2764	prep	of,binding
R2441	T2704	T2690	punct	.,is
R2442	T2705	T2709	advmod	Consistently,hosts
R2443	T2706	T2709	punct	",",hosts
R2444	T2766	T2769	det	the,envelope
R2445	T2707	T2709	amod	non-human,hosts
R2446	T2708	T2709	compound	primate,hosts
R2447	T2709	T2709	ROOT	hosts,hosts
R2448	T2767	T2769	nmod	gp120,envelope
R2449	T2710	T2709	prep	for,hosts
R2450	T2711	T2713	amod	simian,virus
R2451	T2712	T2713	compound	immunodeficiency,virus
R2452	T2713	T2710	pobj	virus,for
R2453	T2768	T2769	amod	viral,envelope
R2454	T2714	T2709	punct	",",hosts
R2455	T2715	T2716	amod	such,as
R2456	T2716	T2709	prep	as,hosts
R2457	T2717	T2719	amod	African,monkeys
R2458	T2769	T2765	pobj	envelope,of
R2459	T2718	T2719	amod	green,monkeys
R2460	T2719	T2716	pobj	monkeys,as
R2461	T2720	T2719	cc	and,monkeys
R2462	T2721	T2722	amod	sooty,mangabey
R2463	T2770	T2772	advmod	to,induces
R2464	T2722	T2719	conj	mangabey,monkeys
R2465	T2723	T2709	punct	",",hosts
R2466	T2724	T2709	conj	lack,hosts
R2467	T2771	T2772	nsubj	CD4,induces
R2468	T2725	T2727	amod	aberrant,activation
R2469	T2726	T2727	amod	immune,activation
R2470	T2727	T2724	dobj	activation,lack
R2471	T2728	T2724	cc	and,lack
R2472	T2772	T2772	ROOT	induces,induces
R2473	T2773	T2775	det	the,activity
R2475	T2774	T2775	amod	NF-κB,activity
R2479	T2775	T2772	dobj	activity,induces
R2483	T2776	T2775	prep	by,activity
R2486	T2777	T2776	pobj	activation,by
R2489	T2778	T2777	prep	of,activation
R2490	T2779	T2778	pobj	IKK,of
R2492	T2741	T2752	advmod	Thus,provide
R2493	T2780	T2779	punct	(,IKK
R2494	T2742	T2752	punct	",",provide
R2495	T2743	T2752	csubj	understanding,provide
R2496	T2781	T2779	appos	30,IKK
R2497	T2744	T2745	det	the,mechanisms
R2498	T2745	T2743	dobj	mechanisms,understanding
R2499	T2746	T2745	prep	of,mechanisms
R2500	T2782	T2779	punct	),IKK
R2501	T2747	T2748	amod	NF-κB,deregulation
R2502	T2748	T2746	pobj	deregulation,of
R2503	T2749	T2745	prep	by,mechanisms
R2504	T2750	T2749	pobj	HIV-1,by
R2505	T2751	T2752	aux	may,provide
R2506	T2752	T2752	ROOT	provide,provide
R2507	T2783	T2777	cc	and,activation
R2508	T2753	T2754	amod	further,insights
R2509	T2754	T2752	dobj	insights,provide
R2510	T2755	T2752	prep	into,provide
R2511	T2784	T2777	conj	procaspase,activation
R2512	T2756	T2757	compound	AIDS,pathogenesis
R2513	T2757	T2755	pobj	pathogenesis,into
R2514	T2758	T2752	punct	.,provide
R2515	T2785	T2784	nummod	8,procaspase
R2516	T2759	T2772	prep	In,induces
R2517	T2760	T2761	compound	HIV-1,entry
R2518	T2761	T2759	pobj	entry,In
R2519	T2762	T2772	punct	",",induces
R2520	T2786	T2787	punct	(,31
R2521	T2763	T2764	det	the,binding
R2522	T2764	T2772	nsubj	binding,induces
R2523	T2787	T2784	npadvmod	31,procaspase
R2524	T2788	T2772	punct	),induces
R2525	T2789	T2772	punct	.,induces
R2526	T2790	T2800	prep	Following,interacts
R2527	T2830	T2829	punct	",",transcripts
R2528	T2831	T2829	prep	including,transcripts
R2529	T2791	T2792	amod	viral,integration
R2530	T2832	T2835	det	the,element
R2531	T2833	T2835	nmod	HIV-1,element
R2532	T2792	T2790	pobj	integration,Following
R2533	T2834	T2835	amod	transactivation-responsive,element
R2534	T2835	T2831	pobj	element,including
R2535	T2836	T2835	punct	(,element
R2536	T2837	T2835	appos	TAR,element
R2537	T2793	T2800	punct	",",interacts
R2538	T2838	T2835	punct	),element
R2539	T2839	T2835	punct	(,element
R2540	T2794	T2799	det	the,protein
R2541	T2840	T2835	appos	32,element
R2542	T2841	T2835	punct	),element
R2543	T2842	T2835	punct	",",element
R2544	T2795	T2799	amod	early,protein
R2545	T2843	T2844	compound	tumor,necrosis
R2546	T2844	T2845	compound	necrosis,factor
R2547	T2796	T2799	amod	encoded,protein
R2548	T2845	T2846	compound	factor,β
R2549	T2846	T2835	appos	β,element
R2550	T2847	T2846	punct	(,β
R2551	T2797	T2798	compound	HIV-1,Tat
R2552	T2848	T2846	appos	TNFβ,β
R2553	T2849	T2846	punct	),β
R2554	T2850	T2846	punct	(,β
R2555	T2851	T2846	appos	33,β
R2556	T2852	T2846	punct	),β
R2557	T2798	T2799	compound	Tat,protein
R2558	T2853	T2846	cc	and,β
R2559	T2854	T2846	conj	interleukin-6,β
R2560	T2799	T2800	nsubj	protein,interacts
R2561	T2855	T2856	punct	(,IL-6
R2562	T2856	T2854	appos	IL-6,interleukin-6
R2563	T2857	T2854	punct	),interleukin-6
R2564	T2800	T2800	ROOT	interacts,interacts
R2565	T2858	T2854	punct	(,interleukin-6
R2566	T2859	T2854	appos	34,interleukin-6
R2567	T2860	T2854	punct	),interleukin-6
R2568	T2801	T2800	prep	with,interacts
R2569	T2861	T2862	aux	to,activate
R2570	T2802	T2804	det	the,RNA
R2571	T2803	T2804	compound	HIV-1,RNA
R2572	T2862	T2835	relcl	activate,element
R2573	T2804	T2801	pobj	RNA,with
R2574	T2863	T2864	compound	gene,transcription
R2575	T2864	T2862	dobj	transcription,activate
R2576	T2865	T2816	punct	.,binds
R2577	T2805	T2804	cc	and,RNA
R2578	T2866	T2869	advmod	Indeed,promotes
R2579	T2867	T2869	punct	",",promotes
R2580	T2868	T2869	nsubj	Tat,promotes
R2581	T2806	T2807	compound	host,cell
R2582	T2869	T2869	ROOT	promotes,promotes
R2583	T2870	T2872	det	the,initiation
R2584	T2871	T2872	amod	transcriptional,initiation
R2585	T2807	T2808	compound	cell,factors
R2586	T2872	T2869	dobj	initiation,promotes
R2587	T2873	T2872	cc	and,initiation
R2588	T2874	T2872	conj	elongation,initiation
R2589	T2808	T2804	conj	factors,RNA
R2590	T2875	T2869	prep	by,promotes
R2591	T2876	T2875	pcomp	interacting,by
R2592	T2877	T2876	prep	with,interacting
R2593	T2878	T2879	amod	transacting,factors
R2594	T2809	T2810	aux	to,sustain
R2595	T2879	T2877	pobj	factors,with
R2596	T2880	T2879	cc	and,factors
R2597	T2881	T2879	conj	cofactors,factors
R2598	T2810	T2800	xcomp	sustain,interacts
R2599	T2882	T2881	punct	",",cofactors
R2600	T2883	T2884	amod	such,as
R2601	T2884	T2879	prep	as,factors
R2602	T2885	T2884	pobj	Sp1,as
R2603	T2886	T2885	punct	(,Sp1
R2604	T2811	T2813	det	the,replication
R2605	T2812	T2813	amod	viral,replication
R2609	T2813	T2810	dobj	replication,sustain
R2613	T2814	T2800	punct	.,interacts
R2616	T2815	T2816	nsubj	Tat,binds
R2619	T2816	T2816	ROOT	binds,binds
R2621	T2817	T2816	prep	to,binds
R2625	T2818	T2817	pobj	RNA,to
R2629	T2819	T2820	amod	stem-loop,structures
R2633	T2820	T2818	dobj	structures,RNA
R2634	T2907	T2906	nummod	T1/CDK9,cyclin
R2635	T2908	T2909	punct	(,"39,40"
R2636	T2909	T2906	appos	"39,40",cyclin
R2637	T2821	T2820	acl	generated,structures
R2638	T2910	T2906	punct	),cyclin
R2639	T2911	T2906	cc	and,cyclin
R2640	T2912	T2913	det	the,histone
R2641	T2822	T2821	agent	by,generated
R2642	T2913	T2906	conj	histone,cyclin
R2643	T2914	T2869	conj	acetyltransferases,promotes
R2644	T2915	T2914	appos	p300/CBP,acetyltransferases
R2645	T2916	T2915	cc	and,p300/CBP
R2646	T2823	T2826	det	the,end
R2647	T2917	T2915	conj	P/CAF,p300/CBP
R2648	T2918	T2917	punct	(,P/CAF
R2649	T2919	T2917	appos	41,P/CAF
R2650	T2920	T2917	appos	43,P/CAF
R2651	T2824	T2825	nummod	5,′
R2652	T2921	T2917	punct	),P/CAF
R2653	T2922	T2869	punct	.,promotes
R2654	T2923	T2924	advmod	When,released
R2655	T2924	T2930	advcl	released,deregulates
R2656	T2925	T2924	prep	from,released
R2657	T2926	T2927	amod	HIV-1-infected,cells
R2658	T2825	T2826	compound	′,end
R2659	T2927	T2925	pobj	cells,from
R2660	T2928	T2930	punct	",",deregulates
R2661	T2929	T2930	nsubj	Tat,deregulates
R2662	T2826	T2822	pobj	end,by
R2663	T2930	T2930	ROOT	deregulates,deregulates
R2664	T2931	T2932	det	the,cell
R2665	T2932	T2930	dobj	cell,deregulates
R2666	T2827	T2826	prep	of,end
R2667	T2933	T2932	acl	signaling,cell
R2668	T2934	T2933	prep	by,signaling
R2669	T2935	T2934	pcomp	binding,by
R2670	T2936	T2935	prep	to,binding
R2671	T2828	T2829	compound	target,transcripts
R2672	T2937	T2938	compound	cell,receptors
R2673	T2938	T2936	pobj	receptors,to
R2674	T2939	T2938	punct	",",receptors
R2675	T2829	T2827	pobj	transcripts,of
R2676	T2940	T2941	amod	such,as
R2677	T2941	T2938	prep	as,receptors
R2678	T2942	T2941	pobj	integrins,as
R2679	T2943	T2942	punct	(,integrins
R2680	T2959	T2961	nsubj	We,reported
R2681	T2944	T2942	appos	44,integrins
R2682	T2945	T2942	punct	),integrins
R2683	T2946	T2938	punct	",",receptors
R2684	T2947	T2948	compound	Flk1/KDR,receptor
R2685	T2960	T2961	advmod	first,reported
R2686	T2948	T2938	appos	receptor,receptors
R2687	T2949	T2948	punct	(,receptor
R2688	T2950	T2948	appos	45,receptor
R2689	T2951	T2948	punct	),receptor
R2690	T2961	T2961	ROOT	reported,reported
R2691	T2952	T2948	cc	and,receptor
R2692	T2953	T2954	amod	chemokine,receptors
R2693	T2954	T2948	conj	receptors,receptor
R2694	T2962	T2964	mark	that,was
R2695	T2955	T2954	punct	(,receptors
R2696	T2956	T2954	appos	46,receptors
R2697	T2957	T2954	punct	),receptors
R2698	T2958	T2930	punct	.,deregulates
R2699	T2963	T2964	nsubj	NF-κB,was
R2700	T2964	T2961	ccomp	was,reported
R2701	T2965	T2966	advmod	constitutively,active
R2702	T2966	T2964	acomp	active,was
R2703	T3056	T3055	pobj	IκB-α,of
R2704	T3057	T3058	punct	(,"15,17"
R2705	T3058	T3056	parataxis	"15,17",IκB-α
R2706	T2967	T2966	prep	in,active
R2707	T3059	T3040	punct	),subvert
R2708	T3060	T3035	punct	.,was
R2709	T3061	T3063	nsubj	We,found
R2710	T2968	T2969	compound	Jurkat,cells
R2711	T3062	T3063	advmod	previously,found
R2712	T3063	T3063	ROOT	found,found
R2713	T3064	T3070	mark	that,promotes
R2714	T2969	T2967	pobj	cells,in
R2715	T3065	T3066	amod	IκB-α,binds
R2716	T3066	T3070	nsubj	binds,promotes
R2717	T3067	T3066	prep	to,binds
R2718	T2970	T2972	nsubj	that,expressed
R2719	T3068	T3067	pobj	Tat,to
R2720	T3069	T3066	cc	and,binds
R2721	T3070	T3063	ccomp	promotes,found
R2722	T2971	T2972	advmod	stably,expressed
R2723	T3071	T3073	det	the,export
R2724	T3072	T3073	amod	nuclear,export
R2725	T2972	T2961	ccomp	expressed,reported
R2726	T3073	T3070	dobj	export,promotes
R2727	T3074	T3073	prep	of,export
R2728	T3075	T3077	det	the,transactivator
R2729	T3076	T3077	amod	viral,transactivator
R2730	T2973	T2975	det	the,gene
R2731	T3077	T3074	pobj	transactivator,of
R2732	T3078	T3077	punct	(,transactivator
R2733	T3079	T3077	appos	"50,51",transactivator
R2734	T2974	T2975	compound	Tat,gene
R2735	T3080	T3077	punct	),transactivator
R2736	T3081	T3063	punct	.,found
R2737	T3082	T3087	prep	In,report
R2738	T3083	T3084	det	this,study
R2739	T3084	T3082	pobj	study,In
R2740	T3085	T3087	punct	",",report
R2741	T3086	T3087	nsubj	we,report
R2742	T3087	T3087	ROOT	report,report
R2743	T2975	T2972	dobj	gene,expressed
R2744	T3088	T3090	mark	that,counteracts
R2745	T3089	T3090	nsubj	Tat,counteracts
R2746	T3090	T3087	ccomp	counteracts,report
R2747	T2976	T2975	punct	(,gene
R2748	T3091	T3093	det	the,turn
R2749	T3092	T3093	amod	post-activation,turn
R2750	T3093	T3090	dobj	turn,counteracts
R2751	T2977	T2975	appos	47,gene
R2752	T3094	T3093	prt	off,turn
R2753	T3095	T3093	prep	of,turn
R2754	T3096	T3095	pobj	NF-κB,of
R2755	T3097	T3090	prep	through,counteracts
R2756	T3098	T3099	amod	direct,interaction
R2757	T2978	T2975	punct	),gene
R2758	T3099	T3097	pobj	interaction,through
R2759	T3100	T3099	prep	with,interaction
R2760	T3101	T3100	pobj	IκB-α,with
R2761	T3102	T3101	cc	and,IκB-α
R2762	T2979	T2961	punct	.,reported
R2763	T3103	T3101	conj	p65,IκB-α
R2764	T3104	T3101	punct	",",IκB-α
R2765	T3105	T3106	nsubj	which,enhances
R2766	T2980	T2988	prep	Following,induced
R2767	T3106	T3101	relcl	enhances,IκB-α
R2768	T3107	T3108	det	the,DNA
R2769	T3108	T3106	dobj	DNA,enhances
R2770	T2981	T2982	compound	gene,transfection
R2771	T2982	T2980	pobj	transfection,Following
R2772	T3109	T3112	amod	binding,activity
R2773	T2983	T2982	cc	or,transfection
R2774	T3110	T3109	cc	and,binding
R2775	T3111	T3109	conj	transcriptional,binding
R2776	T3112	T3106	dobj	activity,enhances
R2777	T2984	T2985	compound	protein,transduction
R2778	T3113	T3112	prep	of,activity
R2779	T3114	T3116	det	the,complex
R2780	T3115	T3116	compound	NF-κB,complex
R2781	T3116	T3113	pobj	complex,of
R2782	T2985	T2982	conj	transduction,transfection
R2783	T3117	T3087	punct	.,report
R2784	T3118	T3120	det	The,mechanism
R2785	T3119	T3120	amod	new,mechanism
R2786	T2986	T2988	punct	",",induced
R2787	T3120	T3125	nsubj	mechanism,described
R2788	T3121	T3120	prep	of,mechanism
R2789	T2987	T2988	nsubj	Tat,induced
R2790	T3122	T3123	amod	NF-κB,deregulation
R2791	T2988	T2988	ROOT	induced,induced
R2792	T3123	T3121	pobj	deregulation,of
R2793	T3124	T3120	advmod	here,mechanism
R2794	T3125	T3125	ROOT	described,described
R2795	T2989	T2991	det	the,activity
R2796	T3126	T3127	aux	may,provide
R2797	T3127	T3125	ccomp	provide,described
R2798	T2990	T2991	compound	IKK,activity
R2799	T3128	T3129	amod	further,insights
R2800	T3129	T3127	dobj	insights,provide
R2801	T3130	T3129	prep	into,insights
R2802	T2991	T2988	dobj	activity,induced
R2803	T3131	T3134	det	the,activation
R2804	T3132	T3134	amod	chronic,activation
R2805	T3133	T3134	amod	immune,activation
R2806	T3134	T3130	pobj	activation,into
R2807	T3135	T3134	prep	of,activation
R2808	T3136	T3137	amod	HIV-1,infection
R2809	T2992	T2991	cc	and,activity
R2810	T3137	T3135	pobj	infection,of
R2811	T3138	T3125	punct	.,described
R2812	T2993	T2994	amod	proteasomal,degradation
R2813	T2994	T2991	conj	degradation,activity
R2814	T2995	T2994	prep	of,degradation
R2815	T2996	T2995	pobj	IκB-α,of
R2816	T2997	T2996	punct	(,IκB-α
R2817	T2998	T2996	appos	48,IκB-α
R2818	T2999	T2996	punct	),IκB-α
R2819	T3000	T2988	punct	",",induced
R2820	T3001	T2988	cc	and,induced
R2821	T3002	T2988	conj	increased,induced
R2822	T3003	T3006	det	the,activity
R2823	T3004	T3006	nmod	p65,activity
R2824	T3005	T3006	compound	transcriptional,activity
R2825	T3006	T3002	dobj	activity,increased
R2826	T3007	T3002	prep	by,increased
R2827	T3008	T3007	pcomp	inhibiting,by
R2828	T3009	T3011	det	the,deacetylation
R2829	T3010	T3011	amod	SIRT-1-mediated,deacetylation
R2830	T3011	T3008	dobj	deacetylation,inhibiting
R2831	T3012	T3011	prep	of,deacetylation
R2832	T3013	T3014	compound	p65,Lys310
R2833	T3014	T3012	pobj	Lys310,of
R2834	T3015	T3014	punct	(,Lys310
R2835	T3016	T3014	appos	49,Lys310
R2836	T3017	T3014	punct	),Lys310
R2837	T3018	T2988	punct	.,induced
R2838	T3019	T3020	det	These,findings
R2839	T3020	T3021	nsubj	findings,suggested
R2840	T3021	T3035	ccomp	suggested,was
R2841	T3022	T3024	mark	that,modulates
R2842	T3023	T3024	nsubj	Tat,modulates
R2843	T3024	T3021	ccomp	modulates,suggested
R2844	T3025	T3026	amod	crucial,enzymes
R2845	T3026	T3024	dobj	enzymes,modulates
R2846	T3027	T3026	acl	involved,enzymes
R2847	T3028	T3027	prep	in,involved
R2848	T3029	T3030	amod	NF-κB,signaling
R2849	T3030	T3024	prep	signaling,modulates
R2850	T3031	T3035	punct	;,was
R2851	T3032	T3035	advmod	however,was
R2852	T3033	T3035	punct	",",was
R2853	T3034	T3035	nsubj	it,was
R2854	T3035	T3035	ROOT	was,was
R2855	T3036	T3035	acomp	unclear,was
R2856	T3037	T3040	advmod	how,subvert
R2857	T3038	T3040	nsubj	Tat,subvert
R2858	T3039	T3040	aux	could,subvert
R2859	T3040	T3035	ccomp	subvert,was
R2860	T3041	T3043	det	the,feedback
R2861	T3042	T3043	amod	negative,feedback
R2871	T3052	T3054	nmod	de,synthesis
R2872	T3053	T3054	amod	novo,synthesis
R2873	T3054	T3051	pobj	synthesis,on
R2874	T3055	T3054	prep	of,synthesis
R4150	T4596	T4596	ROOT	Plasmids,Plasmids
R4151	T4597	T4599	det	The,pcDNA-3xHA-IκB-α
R4152	T4598	T4599	compound	plasmids,pcDNA-3xHA-IκB-α
R4153	T4599	T4596	appos	pcDNA-3xHA-IκB-α,Plasmids
R4154	T4600	T4599	punct	",",pcDNA-3xHA-IκB-α
R4155	T4601	T4599	conj	p3xFLAG-CMV-Tat,pcDNA-3xHA-IκB-α
R4156	T4602	T4601	punct	",",p3xFLAG-CMV-Tat
R4157	T4603	T4604	compound	p3xFLAG-CMV-Tat,C
R4158	T4604	T4599	appos	C,pcDNA-3xHA-IκB-α
R4159	T4605	T4604	punct	(,C
R4160	T4606	T4604	appos	"22,25,27",C
R4161	T4607	T4604	punct	),C
R4162	T4608	T4604	appos	A,C
R4163	T4609	T4611	punct	",",R
R4164	T4610	T4611	amod	p3xFLAG-CMV-Tat,R
R4165	T4611	T4604	appos	R,C
R4166	T4612	T4611	punct	(,R
R4167	T4613	T4611	appos	"49,52,53,55,56,57",R
R4168	T4614	T4611	punct	),R
R4169	T4615	T4620	det	A,C
R4170	T4616	T4617	punct	",",pGEX-2T-Tat
R4171	T4617	T4620	nmod	pGEX-2T-Tat,C
R4172	T4618	T4620	punct	",",C
R4173	T4619	T4620	compound	pGEX-2T-Tat,C
R4174	T4620	T4620	ROOT	C,C
R4175	T4621	T4620	punct	(,C
R4176	T4622	T4620	appos	"22,25,27",C
R4177	T4623	T4620	punct	),C
R4178	T4624	T4624	ROOT	A,A
R4179	T4625	T4624	cc	and,A
R4180	T4626	T4627	amod	pGEX-2T-Tat,R
R4181	T4627	T4624	conj	R,A
R4182	T4628	T4627	punct	(,R
R4183	T4629	T4627	appos	"49,52,53,55,56,57",R
R4184	T4630	T4627	punct	),R
R4185	T4631	T4634	nsubjpass	A,described
R4186	T4632	T4634	auxpass	were,described
R4187	T4633	T4634	advmod	previously,described
R4188	T4634	T4634	ROOT	described,described
R4189	T4635	T4634	punct	(,described
R4190	T4636	T4634	npadvmod	50,described
R4191	T4637	T4634	punct	),described
R4192	T4638	T4634	punct	.,described
R4193	T4639	T4641	det	The,pNL4-3
R4194	T4640	T4641	compound	plasmids,pNL4-3
R4195	T4641	T4648	nsubjpass	pNL4-3,obtained
R4196	T4642	T4641	punct	.,pNL4-3
R4197	T4643	T4648	punct	Luc.R-E,obtained
R4198	T4644	T4643	punct	-,Luc.R-E
R4199	T4645	T4643	cc	and,Luc.R-E
R4200	T4646	T4643	conj	pHXB2-env,Luc.R-E
R4201	T4647	T4648	auxpass	were,obtained
R4202	T4648	T4672	ccomp	obtained,purchased
R4203	T4649	T4648	prep	from,obtained
R4204	T4650	T4656	det	the,Program
R4205	T4651	T4652	compound	AIDS,Research
R4206	T4652	T4656	nmod	Research,Program
R4207	T4653	T4652	cc	&,Research
R4208	T4654	T4652	conj	Reference,Research
R4209	T4655	T4656	compound	Reagent,Program
R4210	T4656	T4649	pobj	Program,from
R4212	T4658	T4656	conj	Division,Program
R4213	T4659	T4658	prep	of,Division
R4214	T4660	T4659	pobj	AIDS,of
R4215	T4661	T4658	punct	",",Division
R4216	T4662	T4658	conj	NIAID,Division
R4217	T4663	T4662	punct	",",NIAID
R4224	T4670	T4668	conj	pSV-β-Gal,pκBluc
R4225	T4671	T4672	auxpass	were,purchased
R4226	T4672	T4672	ROOT	purchased,purchased
R4227	T4673	T4672	prep	from,purchased
R4228	T4674	T4673	pobj	Promega,from
R4229	T4675	T4676	punct	(,Madison
R4230	T4676	T4674	appos	Madison,Promega
R4231	T4677	T4676	punct	",",Madison
R4232	T4678	T4676	conj	WI,Madison
R4233	T4679	T4678	punct	",",WI
R4234	T4680	T4678	npadvmod	USA,WI
R4235	T4681	T4678	punct	),WI
R4236	T4682	T4672	punct	.,purchased
R4237	T4683	T4687	det	The,xHA-p65
R4238	T4684	T4685	compound	plasmids,pRc/CMV
R4239	T4685	T4687	nmod	pRc/CMV,xHA-p65
R4240	T4686	T4687	nummod	-3,xHA-p65
R4241	T4687	T4687	ROOT	xHA-p65,xHA-p65
R4242	T4688	T4687	punct	",",xHA-p65
R4243	T4689	T4687	appos	pRc/CMV,xHA-p65
R4244	T4690	T4691	nummod	-3,xHA-p65ΔC
R4245	T4691	T4687	appos	xHA-p65ΔC,xHA-p65
R4246	T4692	T4693	punct	(,1
R4247	T4693	T4699	nummod	1,xHA-p65ΔN
R4248	T4694	T4693	appos	318,1
R4249	T4695	T4693	punct	),1
R4250	T4696	T4693	punct	",",1
R4251	T4697	T4699	nmod	pRc/CMV,xHA-p65ΔN
R4252	T4698	T4699	nummod	-3,xHA-p65ΔN
R4253	T4699	T4691	appos	xHA-p65ΔN,xHA-p65ΔC
R4254	T4700	T4699	punct	(,xHA-p65ΔN
R4255	T4701	T4699	parataxis	122,xHA-p65ΔN
R4256	T4702	T4701	prep	551,122
R4257	T4703	T4701	punct	),122
R4258	T4704	T4706	punct	",",T
R4259	T4705	T4706	compound	p3xFLAG-CMV-Tat,T
R4260	T4706	T4699	conj	T,xHA-p65ΔN
R4261	T4707	T4706	punct	",",T
R4262	T4708	T4706	npadvmod	N,T
R4263	T4709	T4710	punct	(,"23,24"
R4264	T4710	T4708	parataxis	"23,24",N
R4265	T4711	T4710	punct	),"23,24"
R4266	T4712	T4715	det	A,K
R4267	T4713	T4715	punct	",",K
R4268	T4714	T4715	compound	p3xFLAG-CMV-Tat,K
R4269	T4715	T4715	ROOT	K,K
R4270	T4716	T4715	punct	(,K
R4271	T4717	T4715	appos	"50,51",K
R4272	T4718	T4715	punct	),K
R4273	T4719	T4722	det	A,T
R4274	T4720	T4722	punct	",",T
R4275	T4721	T4722	compound	pGEX-2T-Tat,T
R4276	T4722	T4722	ROOT	T,T
R4277	T4723	T4722	punct	",",T
R4278	T4724	T4722	conj	N,T
R4279	T4725	T4724	punct	(,N
R4280	T4726	T4724	appos	"23,24",N
R4281	T4727	T4724	punct	),N
R4282	T4728	T4728	ROOT	A,A
R4283	T4729	T4735	punct	",",A
R4284	T4730	T4731	amod	pGEX-2T-Tat,K
R4285	T4731	T4735	nmod	K,A
R4286	T4732	T4731	punct	(,K
R4287	T4733	T4731	appos	"50,51",K
R4288	T4734	T4735	punct	),A
R4289	T4735	T4728	det	A,A
R4290	T4736	T4735	cc	and,A
R4291	T4737	T4735	conj	pNL4-3,A
R4292	T4738	T4737	punct	.,pNL4-3
R4293	T4739	T4739	ROOT	FLAG-Tat,FLAG-Tat
R4294	T4740	T4739	punct	.,FLAG-Tat
R4295	T4741	T4744	nsubjpass	R-E,generated
R4296	T4742	T4744	punct	-,generated
R4297	T4743	T4744	auxpass	were,generated
R4298	T4744	T4744	ROOT	generated,generated
R4299	T4745	T4746	mark	as,described
R4300	T4746	T4744	advcl	described,generated
R4301	T4747	T4746	prep	in,described
R4302	T4748	T4749	compound	Supplementary,Data
R4303	T4749	T4747	pobj	Data,in
R4304	T4750	T4744	punct	.,generated
R4581	T5199	T5207	nmod	Cells,HeLa
R4582	T5200	T5199	punct	",",Cells
R4583	T5201	T5199	conj	transfection,Cells
R4584	T5202	T5201	punct	",",transfection
R4585	T5203	T5201	conj	treatments,transfection
R4586	T5204	T5203	cc	and,treatments
R4587	T5205	T5206	compound	luciferase,assay
R4588	T5206	T5203	conj	assay,treatments
R4589	T5207	T5230	nsubjpass	HeLa,cultured
R4590	T5208	T5207	punct	",",HeLa
R4591	T5209	T5219	nummod	p50,fibroblasts
R4592	T5210	T5219	nmod	−,fibroblasts
R4593	T5211	T5219	nmod	/,fibroblasts
R4594	T5212	T5219	nmod	−,fibroblasts
R4595	T5213	T5219	nmod	p65,fibroblasts
R4596	T5214	T5219	nmod	−,fibroblasts
R4597	T5215	T5219	nmod	/,fibroblasts
R4598	T5216	T5219	nmod	−,fibroblasts
R4599	T5217	T5219	nmod	mouse,fibroblasts
R4600	T5218	T5219	amod	embryonic,fibroblasts
R4601	T5219	T5207	appos	fibroblasts,HeLa
R4602	T5220	T5219	punct	(,fibroblasts
R4603	T5221	T5219	appos	MEFs,fibroblasts
R4604	T5222	T5219	punct	),fibroblasts
R4605	T5223	T5219	punct	(,fibroblasts
R4606	T5224	T5219	appos	52,fibroblasts
R4607	T5225	T5219	punct	),fibroblasts
R4608	T5226	T5219	cc	and,fibroblasts
R4609	T5227	T5228	amod	293T,cells
R4610	T5228	T5219	conj	cells,fibroblasts
R4611	T5229	T5230	auxpass	were,cultured
R4612	T5230	T5253	ccomp	cultured,cultured
R4613	T5231	T5230	prep	in,cultured
R4614	T5232	T5237	poss	Dulbecco,medium
R4615	T5233	T5232	case	's,Dulbecco
R4616	T5234	T5237	amod	modified,medium
R4617	T5235	T5237	poss	Eagle,medium
R4618	T5236	T5235	case	's,Eagle
R4619	T5237	T5231	pobj	medium,in
R4620	T5238	T5253	punct	;,cultured
R4621	T5239	T5253	nsubjpass	Jurkat,cultured
R4622	T5240	T5239	punct	",",Jurkat
R4623	T5241	T5242	compound	U937,cells
R4624	T5242	T5239	appos	cells,Jurkat
R4625	T5243	T5242	cc	and,cells
R4626	T5244	T5246	amod	human,blood
R4627	T5245	T5246	compound	peripheral,blood
R4628	T5246	T5248	compound	blood,cells
R4629	T5247	T5248	compound	mononuclear,cells
R4630	T5248	T5242	conj	cells,cells
R4631	T5249	T5250	punct	(,PBMCs
R4632	T5250	T5248	appos	PBMCs,cells
R4633	T5251	T5248	punct	),cells
R4634	T5252	T5253	auxpass	were,cultured
R4635	T5253	T5253	ROOT	cultured,cultured
R4636	T5254	T5253	prep	in,cultured
R4637	T5255	T5254	pobj	RPMI,in
R4638	T5256	T5255	nummod	1640,RPMI
R4639	T5257	T5253	punct	.,cultured
R4640	T5258	T5260	nsubjpass	PBMCs,isolated
R4641	T5259	T5260	auxpass	were,isolated
R4642	T5260	T5260	ROOT	isolated,isolated
R4643	T5261	T5263	mark	as,described
R4644	T5262	T5263	advmod	previously,described
R4645	T5263	T5260	advcl	described,isolated
R4646	T5264	T5263	punct	(,described
R4647	T5265	T5263	npadvmod	53,described
R4648	T5266	T5263	punct	),described
R4649	T5267	T5260	punct	.,isolated
R4650	T5268	T5270	nsubjpass	Media,supplemented
R4651	T5269	T5270	auxpass	were,supplemented
R4652	T5270	T5270	ROOT	supplemented,supplemented
R4653	T5271	T5270	prep	with,supplemented
R4654	T5272	T5273	nummod	10,%
R4655	T5273	T5277	nmod	%,serum
R4656	T5274	T5277	amod	heat-inactivated,serum
R4657	T5275	T5276	amod	fetal,calf
R4658	T5276	T5277	compound	calf,serum
R4659	T5277	T5271	pobj	serum,with
R4660	T5278	T5277	cc	and,serum
R4661	T5279	T5280	nummod	2,mM
R4662	T5280	T5281	compound	mM,l-glutamine
R4663	T5281	T5277	conj	l-glutamine,serum
R4664	T5282	T5285	punct	(,AG
R4665	T5283	T5285	compound	Lonza,AG
R4666	T5284	T5285	compound	Cologne,AG
R4667	T5285	T5277	appos	AG,serum
R4668	T5286	T5285	punct	",",AG
R4669	T5287	T5285	npadvmod	Germany,AG
R4670	T5288	T5285	punct	),AG
R4671	T5289	T5270	punct	.,supplemented
R4672	T5290	T5304	nsubjpass	HeLa,transfected
R4673	T5291	T5290	punct	",",HeLa
R4674	T5292	T5300	nummod	p50,MEFs
R4675	T5293	T5299	nmod	−,−
R4676	T5294	T5299	nmod	/,−
R4677	T5295	T5299	nmod	−,−
R4678	T5296	T5299	nmod	p65,−
R4679	T5297	T5299	nmod	−,−
R4680	T5298	T5299	nmod	/,−
R4681	T5299	T5300	nmod	−,MEFs
R4682	T5300	T5290	conj	MEFs,HeLa
R4683	T5301	T5300	cc	and,MEFs
R4684	T5302	T5300	conj	293T,MEFs
R4685	T5303	T5304	auxpass	were,transfected
R4686	T5304	T5332	ccomp	transfected,equalized
R4687	T5305	T5304	prep	with,transfected
R4688	T5306	T5305	pobj	DNA,with
R4689	T5307	T5304	prep	by,transfected
R4690	T5308	T5307	pcomp	using,by
R4691	T5309	T5310	compound	FuGENE,HD
R4692	T5310	T5308	dobj	HD,using
R4693	T5311	T5314	punct	(,GmbH
R4694	T5312	T5314	compound	Roche,GmbH
R4695	T5313	T5314	compound	Diagnostic,GmbH
R4696	T5314	T5308	dobj	GmbH,using
R4697	T5315	T5314	punct	",",GmbH
R4698	T5316	T5314	conj	Mannheim,GmbH
R4699	T5317	T5316	punct	",",Mannheim
R4700	T5318	T5314	npadvmod	Germany,GmbH
R4701	T5319	T5314	punct	),GmbH
R4702	T5320	T5304	punct	",",transfected
R4703	T5321	T5304	prep	according,transfected
R4704	T5322	T5321	prep	to,according
R4705	T5323	T5324	det	the,manufacturer
R4706	T5324	T5326	poss	manufacturer,protocol
R4707	T5325	T5324	case	's,manufacturer
R4708	T5326	T5322	pobj	protocol,to
R4709	T5327	T5332	punct	;,equalized
R4710	T5328	T5330	amod	total,amounts
R4711	T5329	T5330	compound	DNA,amounts
R4712	T5330	T5332	nsubjpass	amounts,equalized
R4713	T5331	T5332	auxpass	were,equalized
R4714	T5332	T5332	ROOT	equalized,equalized
R4715	T5333	T5332	agent	by,equalized
R4716	T5334	T5333	pobj	transfection,by
R4717	T5335	T5334	prep	of,transfection
R4718	T5336	T5338	nmod	pRc/CMV,vector
R4719	T5337	T5338	amod	empty,vector
R4720	T5338	T5335	pobj	vector,of
R4721	T5339	T5340	punct	(,Invitrogen
R4722	T5340	T5338	appos	Invitrogen,vector
R4723	T5341	T5340	punct	",",Invitrogen
R4724	T5342	T5340	npadvmod	Carlsbad,Invitrogen
R4725	T5343	T5342	punct	",",Carlsbad
R4726	T5344	T5342	conj	CA,Carlsbad
R4727	T5345	T5344	punct	",",CA
R4728	T5346	T5344	conj	USA,CA
R4729	T5347	T5340	punct	),Invitrogen
R4730	T5348	T5332	punct	.,equalized
R4731	T5349	T5355	prep	For,treated
R4732	T5350	T5349	pobj	pulse-stimulation,For
R4733	T5351	T5355	punct	",",treated
R4734	T5352	T5353	compound	HeLa,cells
R4735	T5353	T5355	nsubjpass	cells,treated
R4736	T5354	T5355	auxpass	were,treated
R4737	T5355	T5397	ccomp	treated,washed
R4738	T5356	T5355	prep	with,treated
R4739	T5357	T5359	nmod	phorbol,13-acetate
R4740	T5358	T5359	amod	12-myristate,13-acetate
R4741	T5359	T5356	pobj	13-acetate,with
R4742	T5360	T5359	punct	(,13-acetate
R4743	T5361	T5359	appos	PMA,13-acetate
R4744	T5362	T5397	punct	;,washed
R4745	T5363	T5397	nsubj	Sigma-Aldrich,washed
R4746	T5364	T5363	punct	",",Sigma-Aldrich
R4747	T5365	T5366	compound	St,Louis
R4748	T5366	T5363	conj	Louis,Sigma-Aldrich
R4749	T5367	T5366	punct	",",Louis
R4750	T5368	T5366	conj	MO,Louis
R4751	T5369	T5368	punct	",",MO
R4752	T5370	T5368	conj	USA,MO
R4753	T5371	T5363	punct	),Sigma-Aldrich
R4754	T5372	T5374	punct	(,ng/ml
R4755	T5373	T5374	nummod	20,ng/ml
R4756	T5374	T5363	appos	ng/ml,Sigma-Aldrich
R4757	T5375	T5363	punct	),Sigma-Aldrich
R4758	T5376	T5363	prep	for,Sigma-Aldrich
R4759	T5377	T5378	nummod	5,min
R4760	T5378	T5376	pobj	min,for
R4761	T5379	T5378	punct	",",min
R4762	T5380	T5378	cc	or,min
R4763	T5381	T5382	compound	tumor,necrosis
R4764	T5382	T5383	compound	necrosis,factor-α
R4765	T5383	T5378	conj	factor-α,min
R4766	T5384	T5385	punct	(,TNF-α
R4767	T5385	T5383	appos	TNF-α,factor-α
R4768	T5386	T5363	punct	;,Sigma-Aldrich
R4769	T5387	T5363	conj	Sigma-Aldrich,Sigma-Aldrich
R4770	T5388	T5363	punct	),Sigma-Aldrich
R4771	T5389	T5391	punct	(,ng/ml
R4772	T5390	T5391	nummod	20,ng/ml
R4773	T5391	T5363	appos	ng/ml,Sigma-Aldrich
R4774	T5392	T5363	punct	),Sigma-Aldrich
R4775	T5393	T5363	prep	for,Sigma-Aldrich
R4776	T5394	T5395	nummod	30,min
R4777	T5395	T5393	pobj	min,for
R4778	T5396	T5397	punct	",",washed
R4779	T5397	T5397	ROOT	washed,washed
R4780	T5398	T5397	advmod	twice,washed
R4781	T5399	T5397	prep	in,washed
R4782	T5400	T5402	amod	complete,medium
R4783	T5401	T5402	compound	culture,medium
R4784	T5402	T5399	pobj	medium,in
R4785	T5403	T5397	cc	and,washed
R4786	T5404	T5405	advmod	then,returned
R4787	T5405	T5397	conj	returned,washed
R4788	T5406	T5405	prep	to,returned
R4789	T5407	T5406	pobj	culture,to
R4790	T5408	T5397	punct	.,washed
R4791	T5409	T5415	prep	For,co-transfected
R4792	T5410	T5411	compound	luciferase,assays
R4793	T5411	T5409	pobj	assays,For
R4794	T5412	T5415	punct	",",co-transfected
R4795	T5413	T5414	nsubj	pSV-β-Gal,was
R4796	T5414	T5415	auxpass	was,co-transfected
R4797	T5415	T5415	ROOT	co-transfected,co-transfected
R4798	T5416	T5415	prep	with,co-transfected
R4799	T5417	T5416	pobj	pκBluc,with
R4800	T5418	T5419	aux	to,monitor
R4801	T5419	T5415	advcl	monitor,co-transfected
R4802	T5420	T5422	det	the,efficiency
R4803	T5421	T5422	compound	transfection,efficiency
R4804	T5422	T5419	dobj	efficiency,monitor
R4805	T5423	T5415	punct	.,co-transfected
R4806	T5424	T5425	amod	Forty-eight-hour,post-transfection
R4807	T5425	T5429	intj	post-transfection,lysed
R4808	T5426	T5429	punct	",",lysed
R4809	T5427	T5429	nsubjpass	cells,lysed
R4810	T5428	T5429	auxpass	were,lysed
R4811	T5429	T5429	ROOT	lysed,lysed
R4812	T5430	T5429	prep	in,lysed
R4813	T5431	T5432	compound	lysis,buffer
R4814	T5432	T5430	pobj	buffer,in
R4815	T5433	T5432	prep	of,buffer
R4816	T5434	T5435	compound	Dual,Light
R4817	T5435	T5437	compound	Light,System
R4818	T5436	T5437	compound	Luciferase,System
R4819	T5437	T5433	pobj	System,of
R4820	T5438	T5437	punct	(,System
R4821	T5439	T5437	appos	Tropix,System
R4822	T5440	T5439	punct	",",Tropix
R4823	T5441	T5439	conj	Bedford,Tropix
R4824	T5442	T5441	punct	",",Bedford
R4825	T5443	T5441	conj	MA,Bedford
R4826	T5444	T5443	punct	",",MA
R4827	T5445	T5443	conj	USA,MA
R4828	T5446	T5443	punct	),MA
R4829	T5447	T5437	cc	and,System
R4830	T5448	T5449	det	the,luciferase
R4831	T5449	T5452	nmod	luciferase,activities
R4832	T5450	T5449	cc	and,luciferase
R4833	T5451	T5449	conj	β-galactosidase,luciferase
R4834	T5452	T5454	nsubjpass	activities,evaluated
R4835	T5453	T5454	auxpass	were,evaluated
R4836	T5454	T5429	conj	evaluated,lysed
R4837	T5455	T5454	prep	by,evaluated
R4838	T5456	T5455	pcomp	using,by
R4839	T5457	T5458	compound	Dual,Light
R4840	T5458	T5460	compound	Light,System
R4841	T5459	T5460	compound	Luciferase,System
R4842	T5460	T5456	dobj	System,using
R4843	T5461	T5460	punct	(,System
R4844	T5462	T5460	appos	Tropix,System
R4845	T5463	T5460	punct	),System
R4846	T5464	T5456	prep	in,using
R4847	T5465	T5466	det	a,bioluminometer
R4848	T5466	T5464	pobj	bioluminometer,in
R4849	T5467	T5469	punct	(,Biosystem
R4850	T5468	T5469	compound	Turner,Biosystem
R4851	T5469	T5454	npadvmod	Biosystem,evaluated
R4852	T5470	T5469	punct	",",Biosystem
R4853	T5471	T5469	npadvmod	Sunnyvale,Biosystem
R4854	T5472	T5471	punct	",",Sunnyvale
R4855	T5473	T5471	conj	CA,Sunnyvale
R4856	T5474	T5473	punct	",",CA
R4857	T5475	T5473	conj	USA,CA
R4858	T5476	T5473	punct	),CA
R4859	T5477	T5454	punct	.,evaluated
R4860	T5478	T5479	det	The,ratio
R4861	T5479	T5488	nsubjpass	ratio,expressed
R4862	T5480	T5479	prep	of,ratio
R4863	T5481	T5483	amod	firefly,activity
R4864	T5482	T5483	amod	luciferase,activity
R4865	T5483	T5480	pobj	activity,of
R4866	T5484	T5483	prep	to,activity
R4867	T5485	T5486	amod	β-galactosidase,activity
R4868	T5486	T5484	pobj	activity,to
R4869	T5487	T5488	auxpass	was,expressed
R4870	T5488	T5488	ROOT	expressed,expressed
R4871	T5489	T5488	prep	as,expressed
R4872	T5490	T5492	amod	relative,units
R4873	T5491	T5492	amod	light,units
R4874	T5492	T5489	pobj	units,as
R4875	T5493	T5488	punct	.,expressed
R5028	T5824	T5825	compound	RNA,interference
R5029	T5825	T5826	compound	interference,Jurkat
R5030	T5826	T5829	nmod	Jurkat,cells
R5031	T5827	T5826	cc	or,Jurkat
R5032	T5828	T5826	conj	U937,Jurkat
R5033	T5829	T5831	nsubjpass	cells,transfected
R5034	T5830	T5831	auxpass	were,transfected
R5035	T5831	T5831	ROOT	transfected,transfected
R5036	T5832	T5831	agent	by,transfected
R5037	T5833	T5832	pobj	electroporation,by
R5038	T5834	T5831	advcl	using,transfected
R5039	T5835	T5837	det	a,apparatus
R5040	T5836	T5837	compound	Bio-Rad,apparatus
R5041	T5837	T5834	dobj	apparatus,using
R5042	T5838	T5837	punct	(,apparatus
R5043	T5839	T5840	compound	Bio-Rad,Laboratories
R5044	T5840	T5837	appos	Laboratories,apparatus
R5045	T5841	T5840	punct	",",Laboratories
R5046	T5842	T5840	conj	Hercules,Laboratories
R5047	T5843	T5842	punct	",",Hercules
R5048	T5844	T5842	conj	CA,Hercules
R5049	T5845	T5844	punct	",",CA
R5050	T5846	T5844	conj	USA,CA
R5051	T5847	T5837	punct	),apparatus
R5052	T5848	T5831	punct	.,transfected
R5053	T5849	T5859	advmod	Briefly,suspended
R5054	T5850	T5859	punct	",",suspended
R5055	T5851	T5859	nsubjpass	aliquots,suspended
R5056	T5852	T5851	punct	(,aliquots
R5057	T5853	T5856	nummod	5,cells
R5058	T5854	T5856	nummod	×,cells
R5059	T5855	T5856	nummod	106,cells
R5060	T5856	T5851	appos	cells,aliquots
R5061	T5857	T5851	punct	),aliquots
R5062	T5858	T5859	auxpass	were,suspended
R5063	T5859	T5859	ROOT	suspended,suspended
R5064	T5860	T5859	prep	in,suspended
R5065	T5861	T5862	nummod	0.3,ml
R5066	T5862	T5860	pobj	ml,in
R5067	T5863	T5862	prep	of,ml
R5068	T5864	T5865	compound	RPMI,1640
R5069	T5865	T5863	pobj	1640,of
R5070	T5866	T5859	conj	supplemented,suspended
R5071	T5867	T5866	prep	with,supplemented
R5072	T5868	T5869	nummod	20,%
R5073	T5869	T5872	compound	%,serum
R5074	T5870	T5871	amod	fetal,calf
R5075	T5871	T5872	compound	calf,serum
R5076	T5872	T5867	pobj	serum,with
R5077	T5873	T5872	cc	and,serum
R5078	T5874	T5859	conj	subjected,suspended
R5079	T5875	T5874	prep	to,subjected
R5080	T5876	T5879	det	a,pulse
R5081	T5877	T5879	amod	double,pulse
R5082	T5878	T5879	amod	electrical,pulse
R5083	T5879	T5875	pobj	pulse,to
R5084	T5880	T5882	punct	(,V
R5085	T5881	T5882	nummod	0.22,V
R5086	T5882	T5879	appos	V,pulse
R5087	T5883	T5882	punct	",",V
R5088	T5884	T5885	nummod	960,µF
R5089	T5885	T5882	appos	µF,V
R5090	T5886	T5885	punct	),µF
R5091	T5887	T5882	prep	in,V
R5092	T5888	T5889	det	the,presence
R5093	T5889	T5887	pobj	presence,in
R5094	T5890	T5889	prep	of,presence
R5095	T5891	T5892	compound	annealed,siRNA
R5096	T5892	T5890	pobj	siRNA,of
R5097	T5893	T5892	punct	(,siRNA
R5098	T5894	T5895	nummod	200,pmol
R5099	T5895	T5892	appos	pmol,siRNA
R5100	T5896	T5892	punct	),siRNA
R5101	T5897	T5882	punct	;,V
R5102	T5898	T5899	amod	electroporated,cells
R5103	T5899	T5901	nsubjpass	cells,washed
R5104	T5900	T5901	auxpass	were,washed
R5105	T5901	T5859	conj	washed,suspended
R5106	T5902	T5901	cc	and,washed
R5107	T5903	T5901	conj	cultured,washed
R5108	T5904	T5903	prep	in,cultured
R5109	T5905	T5906	amod	complete,medium
R5110	T5906	T5904	pobj	medium,in
R5111	T5907	T5901	punct	.,washed
R5112	T5908	T5909	compound	RNA,interference
R5113	T5909	T5911	nsubjpass	interference,performed
R5114	T5910	T5911	auxpass	was,performed
R5115	T5911	T5911	ROOT	performed,performed
R5116	T5912	T5911	prep	with,performed
R5117	T5913	T5912	punct	:,with
R5118	T5914	T5915	compound	siRNA,Tat
R5119	T5915	T5916	compound	Tat,sense
R5120	T5916	T5912	pobj	sense,with
R5121	T5917	T5916	punct	",",sense
R5122	T5918	T5916	conj	CUGCUUGUACCAAUUGCUAUU,sense
R5123	T5919	T5918	cc	and,CUGCUUGUACCAAUUGCUAUU
R5124	T5920	T5921	compound	siRNA,Tat
R5125	T5921	T5918	conj	Tat,CUGCUUGUACCAAUUGCUAUU
R5126	T5922	T5918	conj	antisense,CUGCUUGUACCAAUUGCUAUU
R5127	T5923	T5922	punct	",",antisense
R5128	T5924	T5922	conj	UAGCAAUUGGUACAAGCAGUU,antisense
R5129	T5925	T5928	punct	;,sense
R5130	T5926	T5928	compound	siRNA,sense
R5131	T5927	T5928	compound	control,sense
R5132	T5928	T5911	npadvmod	sense,performed
R5133	T5929	T5928	punct	",",sense
R5135	T5931	T5928	appos	AUUGCUAUU,sense
R5136	T5932	T5931	cc	and,AUUGCUAUU
R5137	T5933	T5931	conj	siRNA,AUUGCUAUU
R5138	T5934	T5935	compound	control,antisense
R5139	T5935	T5928	conj	antisense,sense
R5140	T5936	T5935	punct	",",antisense
R5141	T5937	T5928	appos	UAGCAAUUGUGACAAGCAGUU,sense
R5142	T5938	T5911	punct	.,performed
R5143	T5939	T5940	compound	RNA,interference
R5144	T5940	T5946	nsubjpass	interference,performed
R5145	T5941	T5940	prep	of,interference
R5146	T5942	T5941	pobj	p65,of
R5147	T5943	T5940	cc	and,interference
R5148	T5944	T5940	conj	IκB-α,interference
R5149	T5945	T5946	auxpass	was,performed
R5150	T5946	T5946	ROOT	performed,performed
R5151	T5947	T5946	prep	with,performed
R5152	T5948	T5949	compound	SMART,pool
R5153	T5949	T5950	compound	pool,siRNA
R5154	T5950	T5947	pobj	siRNA,with
R5155	T5951	T5950	nummod	p65,siRNA
R5156	T5952	T5950	cc	and,siRNA
R5157	T5953	T5950	conj	IκB-α,siRNA
R5158	T5954	T5955	punct	(,Dharmacon
R5159	T5955	T5950	conj	Dharmacon,siRNA
R5160	T5956	T5955	punct	",",Dharmacon
R5161	T5957	T5955	npadvmod	Chicago,Dharmacon
R5162	T5958	T5955	punct	",",Dharmacon
R5163	T5959	T5955	conj	IL,Dharmacon
R5164	T5960	T5959	punct	",",IL
R5165	T5961	T5959	conj	USA,IL
R5166	T5962	T5946	punct	),performed
R5167	T5963	T5946	punct	.,performed
R5255	T6139	T6140	compound	Pseudotyped,virions
R5256	T6140	T6153	nsubjpass	virions,transfected
R5257	T6141	T6140	cc	and,virions
R5258	T6142	T6146	amod	single,cells
R5259	T6143	T6146	amod	round,cells
R5260	T6144	T6146	nmod	infection,cells
R5261	T6145	T6146	amod	293T,cells
R5262	T6146	T6140	conj	cells,virions
R5263	T6147	T6148	punct	(,1
R5264	T6148	T6150	nummod	1,107
R5265	T6149	T6150	nummod	×,107
R5266	T6150	T6146	appos	107,cells
R5267	T6151	T6146	punct	),cells
R5268	T6152	T6153	auxpass	were,transfected
R5269	T6153	T6153	ROOT	transfected,transfected
R5270	T6154	T6153	prep	with,transfected
R5271	T6155	T6154	pobj	pNL4-3,with
R5272	T6156	T6153	punct	.,transfected
R5273	T6157	T6153	punct	Luc.R-E,transfected
R5274	T6158	T6157	punct	-,Luc.R-E
R5275	T6159	T6157	cc	or,Luc.R-E
R5276	T6160	T6157	conj	pNL4-3,Luc.R-E
R5277	T6161	T6153	punct	.,transfected
R5278	T6162	T6162	ROOT	FLAG-Tat,FLAG-Tat
R5279	T6163	T6162	punct	.,FLAG-Tat
R5280	T6164	T6162	punct	R-E,FLAG-Tat
R5281	T6165	T6164	punct	-,R-E
R5282	T6166	T6164	punct	(,R-E
R5283	T6167	T6168	nummod	10,µg
R5284	T6168	T6164	appos	µg,R-E
R5285	T6169	T6164	punct	),R-E
R5286	T6170	T6171	advmod	together,with
R5287	T6171	T6185	prep	with,collected
R5288	T6172	T6173	compound	pHXB2,Env
R5289	T6173	T6171	pobj	Env,with
R5290	T6174	T6176	punct	(,µg
R5291	T6175	T6176	nummod	10,µg
R5292	T6176	T6173	appos	µg,Env
R5293	T6177	T6176	punct	),µg
R5294	T6178	T6173	punct	",",Env
R5295	T6179	T6173	cc	and,Env
R5296	T6180	T6183	amod	48-h,supernatant
R5297	T6181	T6182	amod	post-transfection,cell
R5298	T6182	T6183	compound	cell,supernatant
R5299	T6183	T6185	nsubjpass	supernatant,collected
R5300	T6184	T6185	auxpass	was,collected
R5301	T6185	T6185	ROOT	collected,collected
R5302	T6186	T6185	punct	.,collected
R5303	T6187	T6189	amod	Enzyme-linked,assay
R5304	T6188	T6189	compound	immunosorbent,assay
R5305	T6189	T6189	ROOT	assay,assay
R5306	T6190	T6189	punct	(,assay
R5307	T6191	T6189	appos	ELISA,assay
R5308	T6192	T6189	punct	),assay
R5309	T6193	T6189	xcomp	using,assay
R5310	T6194	T6195	amod	anti-p24,antibody
R5311	T6195	T6193	dobj	antibody,using
R5312	T6196	T6198	amod	measured,concentration
R5313	T6197	T6198	compound	virion,concentration
R5314	T6198	T6193	dobj	concentration,using
R5315	T6199	T6189	punct	.,assay
R5316	T6200	T6212	nsubjpass	PBMCs,infected
R5317	T6201	T6200	punct	",",PBMCs
R5318	T6202	T6200	conj	Jurkat,PBMCs
R5319	T6203	T6202	cc	or,Jurkat
R5320	T6204	T6202	conj	U937,Jurkat
R5321	T6205	T6202	conj	cells,Jurkat
R5322	T6206	T6205	punct	(,cells
R5323	T6207	T6209	quantmod	5,107
R5324	T6208	T6209	quantmod	×,107
R5325	T6209	T6205	appos	107,cells
R5326	T6210	T6205	punct	),cells
R5327	T6211	T6212	auxpass	were,infected
R5328	T6212	T6212	ROOT	infected,infected
R5329	T6213	T6212	prep	with,infected
R5330	T6214	T6216	nmod	HXB2,virions
R5331	T6215	T6216	compound	Env-pseudotyped,virions
R5332	T6216	T6213	pobj	virions,with
R5333	T6217	T6219	punct	(,ng
R5334	T6218	T6219	nummod	500,ng
R5335	T6219	T6216	appos	ng,virions
R5336	T6220	T6219	prep	of,ng
R5337	T6221	T6220	pobj	p24,of
R5338	T6222	T6219	punct	),ng
R5339	T6223	T6219	prep	by,ng
R5340	T6224	T6223	pobj	spinoculation,by
R5341	T6225	T6219	punct	",",ng
R5342	T6226	T6228	mark	as,described
R5343	T6227	T6228	advmod	previously,described
R5344	T6228	T6219	advcl	described,ng
R5345	T6229	T6228	punct	(,described
R5346	T6230	T6228	npadvmod	50,described
R5347	T6231	T6219	punct	),ng
R5348	T6232	T6212	punct	.,infected
R5358	T6307	T6308	compound	Cell,extracts
R5359	T6308	T6326	nsubjpass	extracts,performed
R5360	T6309	T6308	punct	",",extracts
R5361	T6310	T6311	amod	western,blotting
R5362	T6311	T6326	nsubjpass	blotting,performed
R5363	T6312	T6311	punct	",",blotting
R5364	T6313	T6314	compound	IKK,activity
R5365	T6314	T6311	conj	activity,blotting
R5366	T6315	T6314	cc	and,activity
R5367	T6316	T6317	compound	NF-κB,DNA
R5368	T6317	T6314	conj	DNA,activity
R5369	T6318	T6319	amod	binding,Total
R5370	T6319	T6314	conj	Total,activity
R5371	T6320	T6319	punct	",",Total
R5372	T6321	T6324	amod	nuclear,extracts
R5373	T6322	T6321	cc	and,nuclear
R5374	T6323	T6321	conj	cytosolic,nuclear
R5375	T6324	T6314	conj	extracts,activity
R5376	T6325	T6326	auxpass	were,performed
R5377	T6326	T6336	ccomp	performed,reported
R5378	T6327	T6329	mark	as,described
R5379	T6328	T6329	advmod	previously,described
R5380	T6329	T6326	advcl	described,performed
R5381	T6330	T6329	punct	(,described
R5382	T6331	T6329	npadvmod	54,described
R5383	T6332	T6329	punct	),described
R5384	T6333	T6336	punct	;,reported
R5385	T6334	T6336	nsubjpass	details,reported
R5386	T6335	T6336	auxpass	are,reported
R5387	T6336	T6336	ROOT	reported,reported
R5388	T6337	T6336	prep	in,reported
R5389	T6338	T6339	compound	Supplementary,Data
R5390	T6339	T6337	pobj	Data,in
R5391	T6340	T6336	punct	.,reported
R5392	T6341	T6343	amod	Western,analysis
R5393	T6342	T6343	amod	blotting,analysis
R5394	T6343	T6345	nsubjpass	analysis,performed
R5395	T6344	T6345	auxpass	was,performed
R5396	T6345	T6345	ROOT	performed,performed
R5397	T6346	T6345	agent	by,performed
R5398	T6347	T6380	advcl	resuspending,resolved
R5399	T6348	T6349	compound	protein,aliquots
R5400	T6349	T6347	dobj	aliquots,resuspending
R5401	T6350	T6347	prep	in,resuspending
R5402	T6351	T6350	pcomp	loading,in
R5403	T6352	T6351	dobj	buffer,loading
R5404	T6353	T6352	punct	(,buffer
R5405	T6354	T6356	nummod	125,Tris
R5406	T6355	T6356	compound	mM,Tris
R5407	T6356	T6352	appos	Tris,buffer
R5408	T6357	T6380	nsubj	HCl,resolved
R5409	T6358	T6357	punct	",",HCl
R5410	T6359	T6357	appos	pH,HCl
R5411	T6360	T6359	nummod	6.8,pH
R5412	T6361	T6359	punct	",",pH
R5413	T6362	T6363	nummod	5,%
R5414	T6363	T6364	npadvmod	%,SDS
R5415	T6364	T6357	conj	SDS,HCl
R5416	T6365	T6364	punct	",",SDS
R5417	T6366	T6367	nummod	1,%
R5418	T6367	T6369	npadvmod	%,blue
R5419	T6368	T6369	compound	bromophenol,blue
R5420	T6369	T6373	amod	blue,β-mercaptoethanol
R5421	T6370	T6373	punct	",",β-mercaptoethanol
R5422	T6371	T6372	nummod	10,%
R5423	T6372	T6373	compound	%,β-mercaptoethanol
R5424	T6373	T6364	appos	β-mercaptoethanol,SDS
R5425	T6374	T6364	punct	",",SDS
R5426	T6375	T6376	nummod	25,%
R5427	T6376	T6377	compound	%,glycerol
R5428	T6377	T6364	appos	glycerol,SDS
R5429	T6378	T6364	punct	),SDS
R5430	T6379	T6364	punct	",",SDS
R5431	T6380	T6346	pcomp	resolved,by
R5432	T6381	T6380	prep	on,resolved
R5433	T6382	T6383	nummod	12,%
R5434	T6383	T6384	compound	%,SDS
R5435	T6384	T6381	pobj	SDS,on
R5436	T6385	T6384	appos	PAGE,SDS
R5437	T6386	T6384	punct	",",SDS
R5438	T6387	T6345	advcl	transferred,performed
R5439	T6388	T6389	aux	to,polyvinylidene
R5440	T6389	T6387	xcomp	polyvinylidene,transferred
R5441	T6390	T6391	compound	difluoride,membrane
R5442	T6391	T6389	dobj	membrane,polyvinylidene
R5443	T6392	T6393	punct	(,Millipore
R5444	T6393	T6391	appos	Millipore,membrane
R5445	T6394	T6393	punct	",",Millipore
R5446	T6395	T6393	conj	Bedford,Millipore
R5447	T6396	T6395	punct	",",Bedford
R5448	T6397	T6395	conj	MA,Bedford
R5449	T6398	T6397	punct	",",MA
R5450	T6399	T6397	conj	USA,MA
R5451	T6400	T6397	punct	),MA
R5452	T6401	T6387	cc	and,transferred
R5453	T6402	T6387	conj	incubated,transferred
R5454	T6403	T6402	prep	with,incubated
R5455	T6404	T6405	amod	primary,antibodies
R5456	T6405	T6403	pobj	antibodies,with
R5457	T6406	T6405	punct	(,antibodies
R5458	T6407	T6405	appos	1:1000,antibodies
R5459	T6408	T6405	punct	),antibodies
R5460	T6409	T6387	conj	followed,transferred
R5461	T6410	T6409	agent	by,followed
R5462	T6411	T6410	pobj	incubation,by
R5463	T6412	T6409	prep	with,followed
R5464	T6413	T6414	amod	horseradish-peroxidase-linked,mouse
R5465	T6414	T6417	nmod	mouse,IgG
R5466	T6415	T6414	cc	or,mouse
R5467	T6416	T6414	conj	rabbit,mouse
R5468	T6417	T6412	pobj	IgG,with
R5469	T6418	T6417	punct	(,IgG
R5470	T6419	T6417	appos	1:2000,IgG
R5471	T6420	T6417	punct	),IgG
R5472	T6421	T6424	punct	(,Amersham
R5473	T6422	T6424	compound	GE,Amersham
R5474	T6423	T6424	compound	Healthcare,Amersham
R5475	T6424	T6417	appos	Amersham,IgG
R5476	T6425	T6424	punct	",",Amersham
R5477	T6426	T6427	compound	Little,Chalfont
R5478	T6427	T6424	npadvmod	Chalfont,Amersham
R5479	T6428	T6427	punct	",",Chalfont
R5480	T6429	T6427	conj	Buckinghamshire,Chalfont
R5481	T6430	T6429	punct	",",Buckinghamshire
R5482	T6431	T6429	appos	UK,Buckinghamshire
R5483	T6432	T6429	punct	),Buckinghamshire
R5484	T6433	T6424	prep	in,Amersham
R5485	T6434	T6433	pobj	PBS,in
R5486	T6435	T6417	acl	containing,IgG
R5487	T6436	T6437	nummod	5,%
R5488	T6437	T6440	nmod	%,milk
R5489	T6438	T6440	amod	non-fat,milk
R5490	T6439	T6440	amod	dry,milk
R5491	T6440	T6435	dobj	milk,containing
R5492	T6441	T6443	punct	(,Laboratories
R5493	T6442	T6443	compound	Bio-Rad,Laboratories
R5494	T6443	T6440	appos	Laboratories,milk
R5495	T6444	T6440	punct	),milk
R5496	T6445	T6345	punct	.,performed
R5497	T6446	T6448	nsubjpass	Proteins,detected
R5498	T6447	T6448	auxpass	were,detected
R5499	T6448	T6448	ROOT	detected,detected
R5500	T6449	T6448	agent	by,detected
R5501	T6450	T6449	pobj	chemiluminescence,by
R5502	T6451	T6448	advcl	using,detected
R5503	T6452	T6454	det	the,System
R5504	T6453	T6454	compound	ECL,System
R5505	T6454	T6451	dobj	System,using
R5506	T6455	T6454	punct	(,System
R5507	T6456	T6458	compound	GE,Amersham
R5508	T6457	T6458	compound	Healthcare,Amersham
R5509	T6458	T6454	appos	Amersham,System
R5510	T6459	T6454	punct	),System
R5511	T6460	T6448	punct	.,detected
R5512	T6461	T6462	amod	Primary,antibodies
R5513	T6462	T6464	nsubjpass	antibodies,purchased
R5514	T6463	T6464	auxpass	were,purchased
R5515	T6464	T6464	ROOT	purchased,purchased
R5516	T6465	T6464	prep	from,purchased
R5517	T6466	T6465	punct	:,from
R5518	T6467	T6469	compound	Santa,Biotechnology
R5519	T6468	T6469	compound	Cruz,Biotechnology
R5520	T6469	T6465	pobj	Biotechnology,from
R5521	T6470	T6469	punct	",",Biotechnology
R5522	T6471	T6472	compound	Santa,Cruz
R5523	T6472	T6469	conj	Cruz,Biotechnology
R5524	T6473	T6472	punct	",",Cruz
R5525	T6474	T6472	conj	CA,Cruz
R5526	T6475	T6474	punct	",",CA
R5527	T6476	T6474	conj	USA,CA
R5528	T6477	T6479	punct	(,F7
R5529	T6478	T6479	compound	anti-HA,F7
R5530	T6479	T6474	conj	F7,CA
R5531	T6480	T6479	punct	",",F7
R5532	T6481	T6482	compound	anti-IκB-α,C15
R5533	T6482	T6479	conj	C15,F7
R5534	T6483	T6482	punct	",",C15
R5535	T6484	T6485	compound	anti-Histone,H1
R5536	T6485	T6482	conj	H1,C15
R5537	T6486	T6485	punct	",",H1
R5538	T6487	T6485	conj	anti-Hexokinase-II,H1
R5539	T6488	T6479	punct	),F7
R5540	T6489	T6464	punct	;,purchased
R5541	T6490	T6490	ROOT	Sigma-Aldrich,Sigma-Aldrich
R5542	T6491	T6493	punct	(,M2
R5543	T6492	T6493	amod	anti-FLAG,M2
R5544	T6493	T6490	appos	M2,Sigma-Aldrich
R5545	T6494	T6493	punct	",",M2
R5546	T6495	T6493	amod	anti-γ-Tubulin,M2
R5547	T6496	T6490	punct	),Sigma-Aldrich
R5548	T6497	T6490	punct	;,Sigma-Aldrich
R5549	T6498	T6490	conj	Upstate,Sigma-Aldrich
R5550	T6499	T6498	punct	",",Upstate
R5551	T6500	T6501	compound	Lake,Placid
R5552	T6501	T6498	conj	Placid,Upstate
R5553	T6502	T6501	punct	",",Placid
R5554	T6503	T6501	conj	NY,Placid
R5555	T6504	T6503	punct	",",NY
R5556	T6505	T6503	conj	USA,NY
R5557	T6506	T6507	punct	(,anti-p65
R5558	T6507	T6490	appos	anti-p65,Sigma-Aldrich
R5559	T6508	T6507	punct	),anti-p65
R5560	T6509	T6490	punct	.,Sigma-Aldrich
R5561	T6510	T6515	nsubjpass	Densitometry,analysed
R5562	T6511	T6510	prep	of,Densitometry
R5563	T6512	T6513	amod	single,bands
R5564	T6513	T6511	pobj	bands,of
R5565	T6514	T6515	auxpass	was,analysed
R5566	T6515	T6515	ROOT	analysed,analysed
R5567	T6516	T6515	agent	by,analysed
R5568	T6517	T6519	compound	ImageJ,package
R5569	T6518	T6519	compound	software,package
R5570	T6519	T6516	pobj	package,by
R5571	T6520	T6521	punct	(,NIH
R5572	T6521	T6519	amod	NIH,package
R5573	T6522	T6521	punct	",",NIH
R5574	T6523	T6521	appos	USA,NIH
R5575	T6524	T6521	punct	),NIH
R5576	T6525	T6515	punct	.,analysed
R5577	T6526	T6527	compound	IKK,activity
R5578	T6527	T6529	nsubjpass	activity,evaluated
R5579	T6528	T6529	auxpass	was,evaluated
R5580	T6529	T6529	ROOT	evaluated,evaluated
R5581	T6530	T6529	prep	in,evaluated
R5582	T6531	T6532	amod	cytosolic,extracts
R5583	T6532	T6530	pobj	extracts,in
R5584	T6533	T6529	advcl	using,evaluated
R5585	T6534	T6539	det	the,kit
R5586	T6535	T6539	compound	HTScan,kit
R5587	T6536	T6539	compound	IKK,kit
R5588	T6537	T6538	compound	kinase,assay
R5589	T6538	T6539	compound	assay,kit
R5590	T6539	T6533	dobj	kit,using
R5591	T6540	T6539	punct	(,kit
R5592	T6541	T6543	compound	Cell,Technology
R5593	T6542	T6543	compound	Signaling,Technology
R5594	T6543	T6539	appos	Technology,kit
R5595	T6544	T6543	punct	",",Technology
R5597	T6546	T6545	punct	",",Danvers
R5598	T6547	T6545	conj	MA,Danvers
R5599	T6548	T6547	punct	",",MA
R5600	T6549	T6547	conj	USA,MA
R5601	T6550	T6543	punct	),Technology
R5602	T6551	T6529	punct	.,evaluated
R5603	T6552	T6565	nsubjpass	Binding,measured
R5604	T6553	T6552	prep	of,Binding
R5605	T6554	T6553	pobj	p65,of
R5606	T6555	T6552	punct	",",Binding
R5607	T6556	T6552	appos	p50,Binding
R5608	T6557	T6556	cc	and,p50
R5609	T6558	T6556	conj	FLAG-Tat,p50
R5610	T6559	T6552	prep	to,Binding
R5611	T6560	T6563	det	the,oligonucleotide
R5612	T6561	T6563	amod	double-stranded,oligonucleotide
R5613	T6562	T6563	compound	NF-κB,oligonucleotide
R5614	T6563	T6559	pobj	oligonucleotide,to
R5615	T6564	T6565	auxpass	was,measured
R5616	T6565	T6565	ROOT	measured,measured
R5617	T6566	T6565	advcl	using,measured
R5618	T6567	T6568	compound	NF-κB,Combo
R5619	T6568	T6572	compound	Combo,kit
R5620	T6569	T6572	compound	Transcription,kit
R5621	T6570	T6572	compound	Factor,kit
R5622	T6571	T6572	compound	Assay,kit
R5623	T6572	T6566	dobj	kit,using
R5624	T6573	T6576	punct	(,Company
R5625	T6574	T6576	compound	Cayman,Company
R5626	T6575	T6576	compound	Chemical,Company
R5627	T6576	T6566	parataxis	Company,using
R5628	T6577	T6576	punct	",",Company
R5629	T6578	T6579	compound	Ann,Arbor
R5630	T6579	T6576	conj	Arbor,Company
R5631	T6580	T6579	punct	",",Arbor
R5632	T6581	T6579	conj	MI,Arbor
R5633	T6582	T6581	punct	",",MI
R5634	T6583	T6581	conj	USA,MI
R5635	T6584	T6576	punct	),Company
R5636	T6585	T6565	punct	.,measured
R5637	T6586	T6589	compound	Electrophoretic,Assay
R5638	T6587	T6588	compound	Mobility,Shift
R5639	T6588	T6589	compound	Shift,Assay
R5640	T6589	T6594	nsubjpass	Assay,performed
R5641	T6590	T6591	punct	(,EMSA
R5642	T6591	T6589	appos	EMSA,Assay
R5643	T6592	T6589	punct	),Assay
R5644	T6593	T6594	auxpass	was,performed
R5645	T6594	T6604	ccomp	performed,described
R5646	T6595	T6597	mark	as,described
R5647	T6596	T6597	advmod	previously,described
R5648	T6597	T6594	advcl	described,performed
R5649	T6598	T6597	punct	(,described
R5650	T6599	T6597	npadvmod	55,described
R5651	T6600	T6597	punct	),described
R5652	T6601	T6604	punct	;,described
R5653	T6602	T6604	nsubjpass	details,described
R5654	T6603	T6604	auxpass	are,described
R5655	T6604	T6604	ROOT	described,described
R5656	T6605	T6604	prep	in,described
R5657	T6606	T6607	compound	Supplementary,Data
R5658	T6607	T6605	pobj	Data,in
R5659	T6608	T6604	punct	.,described
R5748	T6765	T6774	prep	In,expressed
R5749	T6766	T6767	amod	vitro,translation
R5750	T6767	T6765	pobj	translation,In
R5751	T6768	T6774	nsubjpass	HA-IκB-α,expressed
R5752	T6769	T6768	punct	",",HA-IκB-α
R5753	T6770	T6768	conj	p65,HA-IκB-α
R5754	T6771	T6770	cc	and,p65
R5755	T6772	T6770	conj	Tat,p65
R5756	T6773	T6774	auxpass	were,expressed
R5757	T6774	T6774	ROOT	expressed,expressed
R5758	T6775	T6774	prep	under,expressed
R5759	T6776	T6778	det	the,promoter
R5760	T6777	T6778	nummod	T7,promoter
R5761	T6778	T6775	pobj	promoter,under
R5762	T6779	T6775	cc	and,under
R5763	T6780	T6775	conj	in,under
R5764	T6781	T6782	amod	vitro,translated
R5765	T6782	T6774	conj	translated,expressed
R5766	T6783	T6782	advcl	using,translated
R5767	T6784	T6789	det	the,system
R5768	T6785	T6789	nmod	TnT,system
R5769	T6786	T6789	amod	quick,system
R5770	T6787	T6789	amod	coupled,system
R5771	T6788	T6789	compound	transcription/translation,system
R5772	T6789	T6783	dobj	system,using
R5773	T6790	T6789	punct	(,system
R5774	T6791	T6789	appos	Promega,system
R5775	T6792	T6789	punct	),system
R5776	T6793	T6783	punct	",",using
R5777	T6794	T6796	mark	as,reported
R5778	T6795	T6796	advmod	previously,reported
R5779	T6796	T6783	advcl	reported,using
R5780	T6797	T6783	punct	(,using
R5781	T6798	T6783	npadvmod	50,using
R5782	T6799	T6783	punct	),using
R5783	T6800	T6774	punct	.,expressed
R5784	T6801	T6803	nsubjpass	Details,described
R5785	T6802	T6803	auxpass	are,described
R5786	T6803	T6803	ROOT	described,described
R5787	T6804	T6803	prep	in,described
R5788	T6805	T6806	compound	Supplementary,Data
R5789	T6806	T6804	pobj	Data,in
R5790	T6807	T6803	punct	.,described
R5860	T6916	T6917	compound	Immunoprecipitation,assay
R5861	T6917	T6934	nsubj	assay,strain
R5862	T6918	T6917	cc	and,assay
R5863	T6919	T6917	conj	GST-pull,assay
R5864	T6920	T6919	prt	down,GST-pull
R5865	T6921	T6919	conj	Immunoprecipitation,GST-pull
R5866	T6922	T6921	punct	",",Immunoprecipitation
R5867	T6923	T6921	conj	GST-pull,Immunoprecipitation
R5868	T6924	T6917	advmod	down,assay
R5869	T6925	T6917	punct	",",assay
R5870	T6926	T6917	cc	and,assay
R5871	T6927	T6917	conj	production,assay
R5872	T6928	T6917	prep	of,assay
R5873	T6929	T6930	compound	GST,proteins
R5874	T6930	T6928	pobj	proteins,of
R5875	T6931	T6917	prep	in,assay
R5876	T6932	T6933	compound	Escherichia,coli
R5877	T6933	T6931	pobj	coli,in
R5878	T6934	T6934	ROOT	strain,strain
R5879	T6935	T6937	nsubjpass	BL21,performed
R5880	T6936	T6937	auxpass	were,performed
R5881	T6937	T6934	ccomp	performed,strain
R5882	T6938	T6940	mark	as,reported
R5883	T6939	T6940	advmod	previously,reported
R5884	T6940	T6937	advcl	reported,performed
R5885	T6941	T6940	punct	(,reported
R5886	T6942	T6940	npadvmod	50,reported
R5887	T6943	T6940	punct	),reported
R5888	T6944	T6934	punct	.,strain
R5889	T6945	T6947	nsubjpass	Details,described
R5890	T6946	T6947	auxpass	are,described
R5891	T6947	T6947	ROOT	described,described
R5892	T6948	T6947	prep	in,described
R5893	T6949	T6950	compound	Supplementary,Data
R5894	T6950	T6948	pobj	Data,in
R5895	T6951	T6947	punct	.,described
R6071	T7201	T7204	amod	Real-time,RNA
R6072	T7202	T7204	compound	PCR,RNA
R6073	T7203	T7204	compound	Total,RNA
R6074	T7204	T7206	nsubjpass	RNA,extracted
R6075	T7205	T7206	auxpass	was,extracted
R6076	T7206	T7226	ccomp	extracted,transcribed
R6077	T7207	T7206	prep	from,extracted
R6078	T7208	T7207	pobj	cells,from
R6079	T7209	T7206	prep	by,extracted
R6080	T7210	T7209	pcomp	using,by
R6081	T7211	T7213	det	the,reagent
R6082	T7212	T7213	compound	TRIzol,reagent
R6083	T7213	T7210	dobj	reagent,using
R6084	T7214	T7213	punct	(,reagent
R6085	T7215	T7213	appos	Invitrogen,reagent
R6086	T7216	T7213	punct	),reagent
R6087	T7217	T7226	punct	;,transcribed
R6088	T7218	T7219	compound	RNA,aliquots
R6089	T7219	T7226	nsubjpass	aliquots,transcribed
R6090	T7220	T7219	punct	(,aliquots
R6091	T7221	T7222	nummod	200,ng
R6092	T7222	T7219	appos	ng,aliquots
R6093	T7223	T7219	punct	),aliquots
R6094	T7224	T7226	auxpass	were,transcribed
R6095	T7225	T7226	advmod	reverse,transcribed
R6096	T7226	T7226	ROOT	transcribed,transcribed
R6097	T7227	T7226	advcl	using,transcribed
R6098	T7228	T7229	compound	Random,Examers
R6099	T7229	T7227	dobj	Examers,using
R6100	T7230	T7229	punct	(,Examers
R6101	T7231	T7229	appos	Roche,Examers
R6102	T7232	T7229	punct	),Examers
R6103	T7233	T7229	cc	and,Examers
R6104	T7234	T7235	compound	Superscript,III
R6105	T7235	T7229	conj	III,Examers
R6106	T7236	T7227	xcomp	Reverse,using
R6107	T7237	T7236	npadvmod	Transcriptase,Reverse
R6108	T7238	T7237	punct	(,Transcriptase
R6109	T7239	T7237	appos	Invitrogen,Transcriptase
R6110	T7240	T7237	punct	),Transcriptase
R6111	T7241	T7236	punct	",",Reverse
R6112	T7242	T7226	prep	according,transcribed
R6113	T7243	T7242	prep	to,according
R6114	T7244	T7245	det	the,manufacturer
R6115	T7245	T7247	poss	manufacturer,protocol
R6116	T7246	T7245	case	's,manufacturer
R6117	T7247	T7243	pobj	protocol,to
R6118	T7248	T7226	punct	.,transcribed
R6119	T7249	T7250	compound	Real-time,PCR
R6120	T7250	T7252	nsubjpass	PCR,performed
R6121	T7251	T7252	auxpass	was,performed
R6122	T7252	T7252	ROOT	performed,performed
R6123	T7253	T7252	prep	with,performed
R6124	T7254	T7258	det	the,mix
R6125	T7255	T7257	compound	iQ,Super
R6126	T7256	T7257	compound	Green,Super
R6127	T7257	T7258	compound	Super,mix
R6128	T7258	T7253	pobj	mix,with
R6129	T7259	T7261	punct	(,Laboratories
R6161	T7291	T7292	compound	°,C
R6162	T7292	T7284	appos	C,C
R6163	T7293	T7292	punct	",",C
R6164	T7294	T7295	nsubj	10,s
R6165	T7295	T7292	appos	s,C
R6166	T7296	T7292	punct	;,C
R6167	T7297	T7298	nummod	60,°
R6168	T7298	T7299	compound	°,C
R6169	T7299	T7292	conj	C,C
R6170	T7300	T7299	punct	",",C
R6171	T7301	T7302	nummod	30,s
R6172	T7302	T7299	appos	s,C
R6173	T7303	T7299	punct	),C
R6174	T7304	T7305	advmod	×,40
R6175	T7305	T7292	appos	40,C
R6176	T7306	T7252	punct	.,performed
R6177	T7307	T7313	nsubjpass	Primers,listed
R6178	T7308	T7307	prep	for,Primers
R6179	T7309	T7308	pobj	Tat,for
R6180	T7310	T7309	cc	and,Tat
R6181	T7311	T7309	conj	MIP-1α,Tat
R6182	T7312	T7313	auxpass	are,listed
R6183	T7313	T7313	ROOT	listed,listed
R6184	T7314	T7313	prep	in,listed
R6185	T7315	T7316	compound	Supplementary,Data
R6186	T7316	T7314	pobj	Data,in
R6187	T7317	T7313	punct	.,listed
R6188	T7318	T7319	amod	Real-time,PCR
R6189	T7319	T7333	nsubjpass	PCR,performed
R6190	T7320	T7319	prep	of,PCR
R6191	T7321	T7320	pobj	CSF3,of
R6192	T7322	T7321	punct	",",CSF3
R6193	T7323	T7321	conj	LTA,CSF3
R6194	T7324	T7323	punct	",",LTA
R6195	T7325	T7323	conj	NFKBIA,LTA
R6196	T7326	T7325	punct	",",NFKBIA
R6197	T7327	T7325	conj	TLR2,NFKBIA
R6198	T7328	T7327	punct	",",TLR2
R6199	T7329	T7327	conj	GAPDH,TLR2
R6200	T7330	T7329	cc	and,GAPDH
R6201	T7331	T7329	conj	ACTB,GAPDH
R6202	T7332	T7333	auxpass	was,performed
R6203	T7333	T7333	ROOT	performed,performed
R6204	T7334	T7333	advcl	using,performed
R6205	T7335	T7337	det	the,profiler
R6206	T7336	T7337	compound	RT2,profiler
R6207	T7337	T7342	nmod	profiler,pathway
R6208	T7338	T7340	compound	PCR,NF-κB
R6209	T7339	T7340	compound	Array-Human,NF-κB
R6210	T7340	T7337	appos	NF-κB,profiler
R6211	T7341	T7342	compound	signaling,pathway
R6212	T7342	T7334	dobj	pathway,using
R6213	T7343	T7342	punct	(,pathway
R6214	T7344	T7345	compound	QIAGEN,Sciences
R6215	T7345	T7342	appos	Sciences,pathway
R6216	T7346	T7345	punct	",",Sciences
R6217	T7347	T7345	conj	MD,Sciences
R6218	T7348	T7345	punct	",",Sciences
R6219	T7349	T7345	appos	USA,Sciences
R6220	T7350	T7342	punct	),pathway
R6221	T7351	T7333	punct	.,performed
R6222	T7352	T7354	nsubjpass	Reactions,carried
R6223	T7353	T7354	auxpass	were,carried
R6224	T7354	T7354	ROOT	carried,carried
R6225	T7355	T7354	prt	out,carried
R6226	T7356	T7354	prep	in,carried
R6227	T7357	T7356	pobj	triplicate,in
R6228	T7358	T7354	punct	",",carried
R6229	T7359	T7354	cc	and,carried
R6230	T7360	T7361	compound	gene,expression
R6231	T7361	T7362	compound	expression,levels
R6232	T7362	T7364	nsubjpass	levels,calculated
R6233	T7363	T7364	auxpass	were,calculated
R6234	T7364	T7354	conj	calculated,carried
R6235	T7365	T7364	oprd	relative,calculated
R6236	T7366	T7365	prep	to,relative
R6237	T7367	T7369	compound	GAPDH,levels
R6238	T7368	T7369	compound	mRNA,levels
R6239	T7369	T7366	pobj	levels,to
R6240	T7370	T7365	prep	as,relative
R6241	T7371	T7372	amod	endogenous,control
R6242	T7372	T7370	pobj	control,as
R6243	T7373	T7364	punct	.,calculated
R6244	T7374	T7375	amod	Relative,expression
R6245	T7375	T7377	nsubjpass	expression,calculated
R6246	T7376	T7377	auxpass	was,calculated
R6247	T7377	T7377	ROOT	calculated,calculated
R6248	T7378	T7377	prep	as,calculated
R6249	T7379	T7382	nummod	2,gene
R6250	T7380	T7382	punct	(,gene
R6251	T7381	T7382	compound	Ct,gene
R6252	T7382	T7378	pobj	gene,as
R6253	T7383	T7382	prep	under,gene
R6254	T7384	T7383	pobj	investigation,under
R6255	T7385	T7387	compound	−,GAPDH
R6256	T7386	T7387	compound	Ct,GAPDH
R6257	T7387	T7382	appos	GAPDH,gene
R6258	T7388	T7382	punct	),gene
R6259	T7389	T7377	punct	.,calculated
R6267	T7546	T7549	amod	Chromatin,Cells
R6268	T7547	T7549	compound	immunoprecipitation,Cells
R6269	T7548	T7549	compound	assay,Cells
R6270	T7549	T7551	nsubjpass	Cells,fixed
R6271	T7550	T7551	auxpass	were,fixed
R6272	T7551	T7551	ROOT	fixed,fixed
R6273	T7552	T7551	prep	by,fixed
R6274	T7553	T7552	pcomp	adding,by
R6275	T7554	T7553	dobj	formaldehyde,adding
R6276	T7555	T7556	punct	(,Sigma-Aldrich
R6277	T7556	T7554	appos	Sigma-Aldrich,formaldehyde
R6278	T7557	T7556	punct	),Sigma-Aldrich
R6279	T7558	T7553	prep	at,adding
R6280	T7559	T7561	det	the,concentration
R6281	T7560	T7561	amod	final,concentration
R6282	T7561	T7558	pobj	concentration,at
R6283	T7562	T7561	prep	of,concentration
R6284	T7563	T7564	nummod	1,%
R6285	T7564	T7562	pobj	%,of
R6286	T7565	T7551	punct	.,fixed
R6287	T7566	T7577	prep	After,added
R6288	T7567	T7568	nummod	10,min
R6289	T7568	T7566	pobj	min,After
R6290	T7569	T7577	punct	",",added
R6291	T7570	T7571	amod	ice-cold,PBS
R6292	T7571	T7577	nsubjpass	PBS,added
R6293	T7572	T7571	cc	plus,PBS
R6294	T7573	T7575	nummod	0.125,glycine
R6295	T7574	T7575	compound	M,glycine
R6296	T7575	T7571	conj	glycine,PBS
R6297	T7576	T7577	auxpass	was,added
R6298	T7577	T7577	ROOT	added,added
R6299	T7578	T7577	punct	",",added
R6300	T7579	T7577	cc	and,added
R6301	T7580	T7582	nsubjpass	plates,transferred
R6302	T7581	T7582	auxpass	were,transferred
R6303	T7582	T7577	conj	transferred,added
R6304	T7583	T7582	prep	on,transferred
R6305	T7584	T7583	pobj	ice,on
R6306	T7585	T7582	punct	",",transferred
R6307	T7586	T7582	conj	washed,transferred
R6308	T7587	T7586	advmod	extensively,washed
R6309	T7588	T7586	prep	with,washed
R6310	T7589	T7588	pobj	PBS,with
R6311	T7590	T7586	punct	",",washed
R6312	T7591	T7586	cc	and,washed
R6313	T7592	T7586	conj	scraped,washed
R6314	T7593	T7577	punct	.,added
R6315	T7594	T7598	prep	After,were
R6316	T7595	T7594	pobj	centrifugation,After
R6317	T7596	T7598	punct	",",were
R6318	T7597	T7598	nsubj	cells,were
R6319	T7598	T7620	ccomp	were,supplemented
R6320	T7599	T7600	nummod	10,min
R6321	T7600	T7598	attr	min,were
R6322	T7601	T7600	amod	lysed,min
R6323	T7602	T7601	prep	in,lysed
R6324	T7603	T7604	compound	lysis,buffer
R6325	T7604	T7602	pobj	buffer,in
R6326	T7605	T7620	punct	(,supplemented
R6327	T7606	T7620	punct	5,supplemented
R6328	T7607	T7609	compound	mM,pH
R6329	T7608	T7609	compound	PIPES,pH
R6330	T7609	T7620	nsubj	pH,supplemented
R6331	T7610	T7609	nummod	8.0,pH
R6332	T7611	T7609	punct	",",pH
R6333	T7612	T7614	nummod	85,KCl
R6334	T7613	T7614	compound	mM,KCl
R6335	T7614	T7609	conj	KCl,pH
R6336	T7615	T7614	punct	",",KCl
R6337	T7616	T7617	nummod	0.5,%
R6338	T7617	T7618	compound	%,NP-40
R6339	T7618	T7614	appos	NP-40,KCl
R6340	T7619	T7620	punct	),supplemented
R6341	T7620	T7620	ROOT	supplemented,supplemented
R6342	T7621	T7620	prep	with,supplemented
R6343	T7622	T7623	nummod	1,×
R6344	T7623	T7626	nummod	×,Inhibitor
R6345	T7624	T7626	amod	Complete,Inhibitor
R6346	T7625	T7626	compound	Protease,Inhibitor
R6347	T7626	T7621	pobj	Inhibitor,with
R6348	T7627	T7630	punct	(,GmbH
R6349	T7628	T7630	compound	Roche,GmbH
R6350	T7629	T7630	compound	Diagnostic,GmbH
R6351	T7630	T7626	appos	GmbH,Inhibitor
R6352	T7631	T7630	punct	),GmbH
R6353	T7632	T7620	punct	.,supplemented
R6354	T7633	T7635	nsubjpass	Nuclei,pelletted
R6355	T7634	T7635	auxpass	were,pelletted
R6356	T7635	T7635	ROOT	pelletted,pelletted
R6357	T7636	T7635	punct	(,pelletted
R6358	T7637	T7638	nummod	1000,×
R6359	T7638	T7639	compound	×,g
R6360	T7639	T7644	dep	g,","
R6361	T7640	T7642	punct	",",min
R6362	T7641	T7642	nummod	5,min
R6363	T7642	T7639	appos	min,g
R6364	T7643	T7639	punct	),g
R6365	T7644	T7635	punct	",",pelletted
R6366	T7645	T7635	cc	and,pelletted
R6367	T7646	T7635	conj	resuspended,pelletted
R6368	T7647	T7646	prep	in,resuspended
R6369	T7648	T7649	compound	sonication,buffer
R6370	T7649	T7647	pobj	buffer,in
R6371	T7650	T7653	punct	(,Tris
R6372	T7651	T7653	nummod	50,Tris
R6373	T7652	T7653	compound	mM,Tris
R6374	T7653	T7646	dep	Tris,resuspended
R6375	T7654	T7655	compound	HCl,pH
R6376	T7655	T7653	appos	pH,Tris
R6377	T7656	T7655	nummod	8.0,pH
R6378	T7657	T7655	punct	",",pH
R6379	T7658	T7659	nummod	1,%
R6380	T7659	T7660	compound	%,SDS
R6381	T7660	T7655	appos	SDS,pH
R6382	T7661	T7660	punct	",",SDS
R6383	T7662	T7664	nummod	10,EDTA
R6384	T7663	T7664	compound	mM,EDTA
R6385	T7664	T7660	appos	EDTA,SDS
R6386	T7665	T7660	punct	),SDS
R6387	T7666	T7653	punct	.,Tris
R6388	T7667	T7669	nsubjpass	Chromatin,sonicated
R6389	T7668	T7669	auxpass	was,sonicated
R6390	T7669	T7669	ROOT	sonicated,sonicated
R6391	T7670	T7669	advcl	using,sonicated
R6392	T7671	T7673	compound	Bandelin,GM70
R6393	T7672	T7673	compound	Sonoplus,GM70
R6394	T7673	T7670	dobj	GM70,using
R6395	T7674	T7673	punct	(,GM70
R6396	T7675	T7676	compound	Bandelin,Electronic
R6397	T7676	T7673	appos	Electronic,GM70
R6398	T7677	T7676	punct	",",Electronic
R6399	T7678	T7676	conj	Berlin,Electronic
R6400	T7679	T7678	punct	",",Berlin
R6401	T7680	T7678	conj	Germany,Berlin
R6402	T7681	T7673	punct	),GM70
R6403	T7682	T7673	punct	",",GM70
R6404	T7683	T7673	amod	centrifuged,GM70
R6405	T7684	T7696	punct	(,was
R6406	T7685	T7686	nummod	14,000
R6407	T7686	T7688	nummod	000,g
R6408	T7687	T7688	compound	×,g
R6409	T7688	T7696	acomp	g,was
R6410	T7689	T7691	punct	",",min
R6411	T7690	T7691	nummod	15,min
R6412	T7691	T7688	appos	min,g
R6413	T7692	T7688	punct	),g
R6414	T7693	T7688	punct	",",g
R6415	T7694	T7688	cc	and,g
R6416	T7695	T7696	nsubj	supernatant,was
R6417	T7696	T7696	ROOT	was,was
R6418	T7697	T7698	advmod	10-fold,diluted
R6419	T7698	T7696	acomp	diluted,was
R6420	T7699	T7698	prep	in,diluted
R6421	T7700	T7701	compound	dilution,buffer
R6422	T7701	T7699	pobj	buffer,in
R6423	T7702	T7705	punct	(,SDS
R6424	T7703	T7704	nummod	0.01,%
R6425	T7704	T7705	npadvmod	%,SDS
R6426	T7705	T7696	parataxis	SDS,was
R6427	T7706	T7705	punct	",",SDS
R6428	T7707	T7709	nummod	16.7,Tris
R6429	T7708	T7709	compound	mM,Tris
R6430	T7709	T7705	conj	Tris,SDS
R6431	T7710	T7711	compound	HCl,pH
R6432	T7711	T7705	npadvmod	pH,SDS
R6433	T7712	T7711	nummod	8.0,pH
R6434	T7713	T7711	punct	",",pH
R6435	T7714	T7715	nummod	1.1,%
R6436	T7715	T7717	compound	%,X-100
R6437	T7716	T7717	compound	Triton,X-100
R6438	T7717	T7711	conj	X-100,pH
R6439	T7718	T7717	punct	",",X-100
R6440	T7719	T7721	nummod	167,NaCl
R6441	T7720	T7721	compound	mM,NaCl
R6442	T7721	T7717	conj	NaCl,X-100
R6443	T7722	T7721	punct	",",NaCl
R6444	T7723	T7725	nummod	1.2,EDTA
R6445	T7724	T7725	compound	mM,EDTA
R6446	T7725	T7721	appos	EDTA,NaCl
R6447	T7726	T7721	punct	),NaCl
R6448	T7727	T7705	punct	.,SDS
R6449	T7728	T7730	nsubjpass	Samples,pre-cleared
R6450	T7729	T7730	auxpass	were,pre-cleared
R6451	T7730	T7730	ROOT	pre-cleared,pre-cleared
R6452	T7731	T7730	agent	by,pre-cleared
R6453	T7732	T7733	amod	3-h,incubation
R6454	T7733	T7731	pobj	incubation,by
R6455	T7734	T7730	prep	with,pre-cleared
R6456	T7735	T7736	nummod	20,µl
R6457	T7736	T7734	pobj	µl,with
R6458	T7737	T7736	prep	of,µl
R6459	T7738	T7741	nmod	protein,beads
R6460	T7739	T7741	nmod	G,beads
R6461	T7740	T7741	amod	agarose,beads
R6462	T7741	T7737	pobj	beads,of
R6463	T7742	T7741	acl	followed,beads
R6464	T7743	T7742	agent	by,followed
R6465	T7744	T7743	pobj	incubation,by
R6466	T7745	T7742	prep	with,followed
R6467	T7746	T7745	pobj	antibodies,with
R6468	T7747	T7746	prep	against,antibodies
R6469	T7748	T7750	det	the,proteins
R6470	T7749	T7750	amod	analysed,proteins
R6471	T7750	T7747	pobj	proteins,against
R6472	T7751	T7730	punct	.,pre-cleared
R6473	T7752	T7753	amod	Primary,antibodies
R6474	T7753	T7754	nsubj	antibodies,were
R6475	T7754	T7754	ROOT	were,were
R6476	T7755	T7754	punct	:,were
R6477	T7756	T7767	amod	anti-p65,IgG
R6478	T7757	T7767	punct	(,IgG
R6479	T7758	T7767	amod	sc-372,IgG
R6480	T7759	T7758	punct	),sc-372
R6481	T7760	T7758	punct	",",sc-372
R6482	T7761	T7758	conj	anti-IκBα,sc-372
R6483	T7762	T7763	punct	(,sc-203
R6484	T7763	T7767	amod	sc-203,IgG
R6485	T7764	T7763	punct	),sc-203
R6486	T7765	T7763	cc	and,sc-203
R6487	T7766	T7763	conj	rabbit,sc-203
R6488	T7767	T7754	attr	IgG,were
R6489	T7768	T7769	punct	(,sc-2027
R6490	T7769	T7767	appos	sc-2027,IgG
R6491	T7770	T7769	punct	),sc-2027
R6492	T7771	T7769	prep	from,sc-2027
R6493	T7772	T7773	compound	Santa,Cruz
R6494	T7773	T7774	compound	Cruz,Biotechnology
R6495	T7774	T7771	pobj	Biotechnology,from
R6496	T7775	T7767	punct	;,IgG
R6497	T7776	T7777	compound	anti-FLAG,M2
R6498	T7777	T7767	conj	M2,IgG
R6499	T7778	T7767	prep	from,IgG
R6500	T7779	T7778	pobj	Sigma,from
R6501	T7780	T7767	conj	Aldrich,IgG
R6502	T7781	T7767	punct	.,IgG
R6503	T7782	T7784	nsubjpass	Immunoprecipitations,carried
R6504	T7783	T7784	auxpass	were,carried
R6505	T7784	T7784	ROOT	carried,carried
R6506	T7785	T7784	prt	out,carried
R6507	T7786	T7784	prep	at,carried
R6508	T7787	T7788	nummod	4,°
R6509	T7788	T7790	nummod	°,overnight
R6510	T7789	T7790	compound	C,overnight
R6511	T7790	T7786	pobj	overnight,at
R6512	T7791	T7790	cc	and,overnight
R6513	T7792	T7790	conj	immune,overnight
R6514	T7793	T7795	nsubjpass	complexes,collected
R6515	T7794	T7795	auxpass	were,collected
R6516	T7795	T7784	conj	collected,carried
R6517	T7796	T7795	prep	with,collected
R6518	T7797	T7800	nmod	protein,beads
R6519	T7798	T7800	nmod	G,beads
R6520	T7799	T7800	amod	agarose,beads
R6521	T7800	T7796	pobj	beads,with
R6522	T7801	T7802	punct	",",washed
R6523	T7802	T7784	conj	washed,carried
R6524	T7803	T7804	nummod	five,times
R6525	T7804	T7802	npadvmod	times,washed
R6526	T7805	T7802	prep	with,washed
R6527	T7806	T7808	amod	low,buffer
R6528	T7807	T7808	compound	salt,buffer
R6529	T7808	T7805	pobj	buffer,with
R6530	T7809	T7808	punct	(,buffer
R6531	T7810	T7812	nummod	20,Tris
R6532	T7811	T7812	compound	mM,Tris
R6533	T7812	T7808	appos	Tris,buffer
R6534	T7813	T7814	compound	HCl,pH
R6535	T7814	T7808	appos	pH,buffer
R6536	T7815	T7814	nummod	8.0,pH
R6537	T7816	T7814	punct	",",pH
R6538	T7817	T7818	nummod	0.1,%
R6539	T7818	T7819	npadvmod	%,SDS
R6540	T7819	T7814	appos	SDS,pH
R6541	T7820	T7819	punct	",",SDS
R6542	T7821	T7822	nummod	1,%
R6543	T7822	T7824	compound	%,X-100
R6544	T7823	T7824	compound	Triton,X-100
R6545	T7824	T7819	conj	X-100,SDS
R6546	T7825	T7824	punct	",",X-100
R6547	T7826	T7828	nummod	2,EDTA
R6548	T7827	T7828	compound	mM,EDTA
R6549	T7828	T7824	conj	EDTA,X-100
R6550	T7829	T7828	punct	",",EDTA
R6551	T7830	T7832	nummod	150,NaCl
R6552	T7831	T7832	compound	mM,NaCl
R6553	T7832	T7828	appos	NaCl,EDTA
R6554	T7833	T7828	punct	),EDTA
R6555	T7834	T7819	punct	",",SDS
R6556	T7835	T7836	nummod	four,times
R6557	T7836	T7819	npadvmod	times,SDS
R6558	T7837	T7819	prep	with,SDS
R6559	T7838	T7840	amod	high,buffer
R6560	T7839	T7840	compound	salt,buffer
R6561	T7840	T7837	pobj	buffer,with
R6562	T7841	T7844	punct	(,Tris
R6563	T7842	T7844	nummod	20,Tris
R6564	T7843	T7844	compound	mM,Tris
R6565	T7844	T7840	appos	Tris,buffer
R6566	T7845	T7846	compound	HCl,pH
R6567	T7846	T7885	nsubj	pH,extracted
R6568	T7847	T7846	nummod	8.0,pH
R6569	T7848	T7846	punct	",",pH
R6570	T7849	T7850	nummod	0.1,%
R6571	T7850	T7851	npadvmod	%,SDS
R6572	T7851	T7846	npadvmod	SDS,pH
R6573	T7852	T7851	punct	",",SDS
R6574	T7853	T7854	nummod	1,%
R6575	T7854	T7856	compound	%,X-100
R6576	T7855	T7856	compound	Triton,X-100
R6577	T7856	T7851	appos	X-100,SDS
R6578	T7857	T7856	punct	",",X-100
R6579	T7858	T7860	nummod	2,EDTA
R6580	T7859	T7860	compound	mM,EDTA
R6581	T7860	T7856	conj	EDTA,X-100
R6582	T7861	T7860	punct	",",EDTA
R6583	T7862	T7864	nummod	500,NaCl
R6584	T7863	T7864	compound	mM,NaCl
R6585	T7864	T7860	appos	NaCl,EDTA
R6586	T7865	T7860	punct	),EDTA
R6587	T7866	T7851	punct	",",SDS
R6588	T7867	T7868	advmod	once,with
R6589	T7868	T7851	prep	with,SDS
R6590	T7869	T7870	compound	TE,buffer
R6591	T7870	T7868	pobj	buffer,with
R6592	T7871	T7851	punct	(,SDS
R6593	T7872	T7874	nummod	10,Tris
R6594	T7873	T7874	compound	mM,Tris
R6595	T7874	T7851	appos	Tris,SDS
R6596	T7875	T7876	compound	HCl,pH
R6597	T7876	T7851	appos	pH,SDS
R6598	T7877	T7876	nummod	8.0,pH
R6599	T7878	T7876	punct	",",pH
R6600	T7879	T7881	nummod	1,EDTA
R6601	T7880	T7881	compound	mM,EDTA
R6602	T7881	T7876	appos	EDTA,pH
R6603	T7882	T7876	punct	),pH
R6604	T7883	T7851	punct	",",SDS
R6605	T7884	T7846	cc	and,pH
R6606	T7885	T7885	ROOT	extracted,extracted
R6607	T7886	T7885	prep	in,extracted
R6608	T7887	T7888	compound	TE,buffer
R6609	T7888	T7886	pobj	buffer,in
R6610	T7889	T7888	acl	containing,buffer
R6611	T7890	T7891	nummod	2,%
R6612	T7891	T7889	dobj	%,containing
R6613	T7892	T7889	npadvmod	SDS,containing
R6614	T7893	T7885	punct	.,extracted
R6615	T7894	T7894	ROOT	Protein,Protein
R6616	T7895	T7896	compound	DNA,cross-links
R6617	T7896	T7898	nsubjpass	cross-links,reverted
R6618	T7897	T7898	auxpass	were,reverted
R6619	T7898	T7898	ROOT	reverted,reverted
R6620	T7899	T7898	agent	by,reverted
R6621	T7900	T7899	pobj	heating,by
R6622	T7901	T7898	prep	at,reverted
R6623	T7902	T7904	nummod	65,C
R6624	T7903	T7904	nummod	°,C
R6625	T7904	T7905	compound	C,overnight
R6626	T7905	T7901	pobj	overnight,at
R6627	T7906	T7898	punct	.,reverted
R6628	T7907	T7910	nsubjpass	DNA,purified
R6629	T7908	T7910	auxpass	was,purified
R6630	T7909	T7910	advmod	further,purified
R6631	T7910	T7910	ROOT	purified,purified
R6650	T7929	T7935	nsubjpass	enrichment,measured
R6651	T7930	T7929	prep	in,enrichment
R6652	T7931	T7933	amod	NF-κB,sequences
R6653	T7932	T7933	compound	enhancer,sequences
R6654	T7933	T7930	pobj	sequences,in
R6655	T7934	T7935	auxpass	was,measured
R6656	T7935	T7935	ROOT	measured,measured
R6657	T7936	T7935	agent	by,measured
R6658	T7937	T7938	amod	real-time,PCR
R6659	T7938	T7936	pobj	PCR,by
R6660	T7939	T7938	prep	of,PCR
R6661	T7940	T7941	compound	chromatin,immunoprecipitation
R6662	T7941	T7939	pobj	immunoprecipitation,of
R6663	T7942	T7943	punct	(,ChIP
R6664	T7943	T7941	appos	ChIP,immunoprecipitation
R6665	T7944	T7941	punct	),immunoprecipitation
R6666	T7945	T7935	oprd	eluates,measured
R6667	T7946	T7945	advcl	using,eluates
R6668	T7947	T7950	compound	SYBR,Mix
R6669	T7948	T7950	compound	GreenER,Mix
R6670	T7949	T7950	compound	Master,Mix
R6671	T7950	T7946	dobj	Mix,using
R6672	T7951	T7950	punct	(,Mix
R6673	T7952	T7950	appos	Invitrogen,Mix
R6674	T7953	T7950	punct	),Mix
R6675	T7954	T7935	punct	.,measured
R6676	T7955	T7957	nsubjpass	Reactions,carried
R6677	T7956	T7957	auxpass	were,carried
R6678	T7957	T7957	ROOT	carried,carried
R6679	T7958	T7957	prt	out,carried
R6680	T7959	T7957	prep	with,carried
R6681	T7960	T7965	det	the,system
R6682	T7961	T7963	compound	iCycler,Real-Time
R6683	T7962	T7963	compound	iQ,Real-Time
R6684	T7963	T7965	compound	Real-Time,system
R6685	T7964	T7965	compound	detection,system
R6686	T7965	T7959	pobj	system,with
R6687	T7966	T7965	punct	(,system
R6688	T7967	T7968	compound	Bio-Rad,Laboratories
R6689	T7968	T7965	appos	Laboratories,system
R6690	T7969	T7965	punct	),system
R6691	T7970	T7957	advcl	using,carried
R6692	T7971	T7973	det	the,conditions
R6693	T7972	T7973	amod	following,conditions
R6694	T7973	T7970	dobj	conditions,using
R6695	T7974	T7973	punct	:,conditions
R6696	T7975	T7976	nummod	95,°
R6697	T7976	T7977	compound	°,C
R6698	T7977	T7973	appos	C,conditions
R6699	T7978	T7977	punct	",",C
R6700	T7979	T7980	nummod	1,min
R6701	T7980	T7977	appos	min,C
R6702	T7981	T7977	punct	;,C
R6703	T7982	T7985	punct	(,C
R6704	T7983	T7984	nummod	94,°
R6705	T7984	T7985	compound	°,C
R6706	T7985	T7977	appos	C,C
R6707	T7986	T7985	punct	",",C
R6708	T7987	T7988	nsubj	10,s
R6709	T7988	T7977	appos	s,C
R6710	T7989	T7977	punct	;,C
R6711	T7990	T7991	nummod	60,°
R6712	T7991	T7992	compound	°,C
R6713	T7992	T7977	conj	C,C
R6714	T7993	T7992	punct	",",C
R6715	T7994	T7995	nummod	30,s
R6716	T7995	T7992	appos	s,C
R6717	T7996	T7992	punct	),C
R6718	T7997	T7998	advmod	×,40
R6719	T7998	T7977	appos	40,C
R6720	T7999	T7957	punct	.,carried
R6721	T8000	T8009	nsubjpass	Primers,listed
R6722	T8001	T8000	acl	used,Primers
R6723	T8002	T8001	prep	for,used
R6724	T8003	T8002	pobj	MIP-1α,for
R6725	T8004	T8003	punct	",",MIP-1α
R6726	T8005	T8003	conj	GAPDH,MIP-1α
R6727	T8006	T8005	cc	and,GAPDH
R6728	T8007	T8005	conj	ACTB,GAPDH
R6729	T8008	T8009	auxpass	are,listed
R6730	T8009	T8009	ROOT	listed,listed
R6731	T8010	T8009	prep	in,listed
R6732	T8011	T8012	compound	Supplementary,Data
R6733	T8012	T8010	pobj	Data,in
R6734	T8013	T8009	punct	.,listed
R6735	T8014	T8015	amod	Real-time,PCR
R6736	T8015	T8025	nsubjpass	PCR,performed
R6737	T8016	T8015	prep	of,PCR
R6738	T8017	T8016	pobj	CSF3,of
R6739	T8018	T8017	punct	",",CSF3
R6740	T8019	T8017	conj	LTA,CSF3
R6741	T8020	T8019	punct	",",LTA
R6742	T8021	T8019	conj	NFKBIA,LTA
R6743	T8022	T8021	cc	and,NFKBIA
R6744	T8023	T8021	conj	TLR2,NFKBIA
R6745	T8024	T8025	auxpass	were,performed
R6746	T8025	T8025	ROOT	performed,performed
R6747	T8026	T8025	advcl	using,performed
R6748	T8027	T8030	det	the,array
R6749	T8028	T8030	compound	Custom,array
R6750	T8029	T8030	compound	ChIP,array
R6751	T8030	T8026	dobj	array,using
R6752	T8031	T8030	punct	(,array
R6753	T8032	T8030	appos	QIAGEN,array
R6754	T8033	T8030	punct	),array
R6755	T8034	T8025	punct	.,performed
R6756	T8035	T8040	prep	For,were
R6757	T8036	T8037	det	each,sample
R6758	T8037	T8035	pobj	sample,For
R6759	T8038	T8040	punct	",",were
R6760	T8039	T8040	nsubj	values,were
R6761	T8040	T8040	ROOT	were,were
R6762	T8041	T8040	acomp	normalized,were
R6763	T8042	T8041	prep	to,normalized
R6764	T8043	T8046	dep	input,reported
R6765	T8044	T8043	dobj	DNA,input
R6766	T8045	T8043	cc	and,input
R6767	T8046	T8040	conj	reported,were
R6768	T8047	T8048	mark	as,%
R6769	T8048	T8046	advcl	%,reported
R6770	T8049	T8048	prep	of,%
R6771	T8050	T8049	pobj	input,of
R6772	T8051	T8048	prep	over,%
R6773	T8052	T8053	det	the,rabbit
R6774	T8053	T8051	pobj	rabbit,over
R6775	T8054	T8055	compound	IgG,control
R6776	T8055	T8048	conj	control,%
R6777	T8056	T8046	punct	.,reported
R6815	T8195	T8196	compound	Statistical,analysis
R6816	T8196	T8198	compound	analysis,analysis
R6817	T8197	T8198	compound	Statistical,analysis
R6818	T8198	T8200	nsubjpass	analysis,performed
R6819	T8199	T8200	auxpass	was,performed
R6820	T8200	T8200	ROOT	performed,performed
R6821	T8201	T8200	agent	by,performed
R6822	T8202	T8204	compound	two-tail,Student
R6823	T8203	T8204	compound	unpaired,Student
R6824	T8204	T8201	pobj	Student,by
R6825	T8205	T8204	case	's,Student
R6826	T8206	T8204	amod	t-test,Student
R6827	T8207	T8200	punct	.,performed
R6828	T8208	T8210	nsubjpass	Data,reported
R6829	T8209	T8210	auxpass	were,reported
R6830	T8210	T8210	ROOT	reported,reported
R6831	T8211	T8210	prep	as,reported
R6832	T8212	T8211	pobj	means,as
R6833	T8213	T8214	compound	±,SE
R6834	T8214	T8212	dobj	SE,means
R6835	T8215	T8210	punct	.,reported
R6836	T8216	T8221	nsubjpass	Differences,considered
R6837	T8217	T8216	prep	between,Differences
R6838	T8218	T8219	det	the,means
R6839	T8219	T8217	pobj	means,between
R6840	T8220	T8221	auxpass	were,considered
R6841	T8221	T8221	ROOT	considered,considered
R6842	T8222	T8221	prep	as,considered
R6843	T8223	T8224	advmod	statistically,significant
R6844	T8224	T8222	amod	significant,as
R6845	T8225	T8224	prep	at,significant
R6846	T8226	T8229	det	the,level
R6847	T8227	T8228	nummod	95,%
R6848	T8228	T8229	compound	%,level
R6849	T8229	T8225	pobj	level,at
R6850	T8230	T8229	punct	(,level
R6851	T8231	T8234	nmod	P,)
R6852	T8232	T8233	compound	<,0.05
R6853	T8233	T8231	nummod	0.05,P
R6854	T8234	T8229	punct	),level
R6855	T8235	T8221	punct	.,considered
R7872	T9532	T9533	nsubj	Tat,enhances
R7873	T9533	T9533	ROOT	enhances,enhances
R7874	T9534	T9536	det	the,activity
R7875	T9535	T9536	amod	NF-κB,activity
R7876	T9536	T9533	dobj	activity,enhances
R7877	T9537	T9536	prep	by,activity
R7878	T9538	T9537	pcomp	hijacking,by
R7879	T9539	T9538	dobj	IκB-α,hijacking
R7880	T9540	T9538	cc	and,hijacking
R7881	T9541	T9538	conj	inhibiting,hijacking
R7882	T9542	T9544	det	the,turn
R7883	T9543	T9544	nsubj	post-activation,turn
R7884	T9544	T9541	dobj	turn,inhibiting
R7885	T9545	T9544	prt	off,turn
R7886	T9546	T9545	prep	of,off
R7887	T9547	T9546	pobj	NF-κB,of
R7888	T9548	T9549	nsubj	We,analysed
R7889	T9549	T9536	relcl	analysed,activity
R7890	T9550	T9551	det	the,kinetic
R7891	T9551	T9549	dobj	kinetic,analysed
R7892	T9552	T9551	prep	of,kinetic
R7893	T9553	T9554	amod	NF-κB,activation
R7894	T9554	T9552	pobj	activation,of
R7895	T9555	T9554	prep	in,activation
R7896	T9556	T9559	amod	single,infection
R7897	T9557	T9559	compound	round,infection
R7898	T9558	T9559	compound	HIV-1,infection
R7899	T9559	T9555	pobj	infection,in
R7900	T9560	T9549	prep	by,analysed
R7901	T9561	T9560	pcomp	modulating,by
R7902	T9562	T9563	det	the,expression
R7903	T9563	T9561	dobj	expression,modulating
R7904	T9564	T9563	prep	of,expression
R7905	T9565	T9564	pobj	Tat,of
R7906	T9566	T9561	prep	with,modulating
R7907	T9567	T9568	compound	RNA,interference
R7908	T9568	T9566	pobj	interference,with
R7909	T9569	T9533	punct	.,enhances
R7910	T9570	T9571	compound	Jurkat,cells
R7911	T9571	T9573	nsubjpass	cells,transfected
R7912	T9572	T9573	auxpass	were,transfected
R7913	T9573	T9573	ROOT	transfected,transfected
R7914	T9574	T9573	prep	with,transfected
R7915	T9575	T9576	compound	siRNA,Tat
R7916	T9576	T9574	pobj	Tat,with
R7917	T9577	T9573	punct	",",transfected
R7918	T9578	T9579	compound	siRNA,control
R7919	T9579	T9573	conj	control,transfected
R7920	T9580	T9579	punct	",",control
R7921	T9581	T9579	cc	or,control
R7922	T9582	T9573	conj	left,transfected
R7923	T9583	T9582	oprd	untransfected,left
R7924	T9584	T9582	punct	",",left
R7925	T9585	T9582	cc	and,left
R7926	T9586	T9589	nummod	24,cells
R7927	T9587	T9589	nmod	h,cells
R7928	T9588	T9589	amod	later,cells
R7929	T9589	T9591	nsubjpass	cells,infected
R7930	T9590	T9591	auxpass	were,infected
R7931	T9591	T9582	conj	infected,left
R7932	T9592	T9591	prep	with,infected
R7933	T9593	T9595	nummod	HXB2,NL4-3
R7934	T9594	T9595	amod	Env-pseudotyped,NL4-3
R7935	T9595	T9592	pobj	NL4-3,with
R7936	T9596	T9591	punct	.,infected
R7937	T9597	T9601	compound	Luc.R,virions
R7938	T9598	T9601	compound	−,virions
R7939	T9599	T9600	compound	E,−
R7940	T9600	T9601	compound	−,virions
R7941	T9601	T9601	ROOT	virions,virions
R7942	T9602	T9601	punct	.,virions
R7943	T9603	T9612	prep	By,was
R7944	T9604	T9605	amod	cytofluorimetric,analysis
R7945	T9605	T9603	pobj	analysis,By
R7946	T9606	T9612	punct	",",was
R7947	T9607	T9612	advmod	∼,was
R7948	T9608	T9609	nummod	40,%
R7949	T9609	T9612	nsubj	%,was
R7950	T9610	T9609	prep	of,%
R7951	T9611	T9610	pobj	cells,of
R7952	T9612	T9612	ROOT	was,was
R7953	T9613	T9612	acomp	siRNA-transfected,was
R7954	T9614	T9613	cc	and,siRNA-transfected
R7955	T9615	T9613	conj	HIV-1-infected,siRNA-transfected
R7956	T9616	T9612	prep	at,was
R7957	T9617	T9618	amod	12-h,post-infection
R7958	T9618	T9616	pobj	post-infection,at
R7959	T9619	T9622	punct	(,S1
R7960	T9620	T9622	compound	Supplementary,S1
R7961	T9621	T9622	compound	Figure,S1
R7962	T9622	T9618	appos	S1,post-infection
R7963	T9623	T9622	punct	),S1
R7964	T9624	T9612	punct	.,was
R7965	T9625	T9626	det	The,expression
R7966	T9626	T9630	nsubjpass	expression,detected
R7967	T9627	T9626	prep	of,expression
R7968	T9628	T9627	pobj	Tat,of
R7969	T9629	T9630	auxpass	was,detected
R7970	T9630	T9630	ROOT	detected,detected
R7971	T9631	T9630	prep	at,detected
R7972	T9632	T9633	compound	1-h,post-infection
R7973	T9633	T9631	pobj	post-infection,at
R7974	T9634	T9630	cc	and,detected
R7975	T9635	T9636	advmod	progressively,increased
R7976	T9636	T9630	conj	increased,detected
R7977	T9637	T9636	prt	up,increased
R7978	T9638	T9636	prep	to,increased
R7979	T9639	T9640	nummod	12,h
R7980	T9640	T9638	pobj	h,to
R7981	T9641	T9640	prep	in,h
R7982	T9642	T9646	amod	untransfected,cells
R7983	T9643	T9642	cc	and,untransfected
R7984	T9644	T9642	conj	siRNA,untransfected
R7985	T9645	T9646	amod	control-transfected,cells
R7986	T9646	T9641	pobj	cells,in
R7987	T9647	T9636	punct	",",increased
R7988	T9648	T9652	mark	while,detected
R7989	T9649	T9652	nsubjpass	it,detected
R7990	T9650	T9652	auxpass	was,detected
R7991	T9651	T9652	advmod	barely,detected
R7992	T9652	T9636	advcl	detected,increased
R7993	T9653	T9652	prep	in,detected
R7994	T9654	T9653	pobj	siRNA,in
R7995	T9655	T9656	amod	Tat-transfected,cells
R7996	T9656	T9652	dobj	cells,detected
R7997	T9657	T9658	punct	(,Figure
R7998	T9658	T9656	parataxis	Figure,cells
R7999	T9659	T9658	nummod	1A,Figure
R8000	T9660	T9658	punct	),Figure
R8001	T9661	T9630	punct	.,detected
R8002	T9662	T9665	det	The,expression
R8003	T9663	T9665	amod	LTR-dependent,expression
R8004	T9664	T9665	compound	luciferase,expression
R8005	T9665	T9669	nsubjpass	expression,induced
R8006	T9666	T9669	auxpass	was,induced
R8007	T9667	T9666	advmod	also,was
R8008	T9668	T9669	advmod	progressively,induced
R8009	T9669	T9669	ROOT	induced,induced
R8010	T9670	T9669	prep	at,induced
R8011	T9671	T9670	pobj	1,at
R8012	T9672	T9669	punct	-,induced
R8013	T9673	T9669	prep	to,induced
R8014	T9674	T9675	amod	12-h,post-infection
R8015	T9675	T9673	pobj	post-infection,to
R8016	T9676	T9675	prep	in,post-infection
R8017	T9677	T9681	amod	untransfected,cells
R8018	T9678	T9677	cc	and,untransfected
R8019	T9679	T9677	conj	siRNA,untransfected
R8020	T9680	T9681	amod	control-transfected,cells
R8021	T9681	T9676	pobj	cells,in
R8022	T9682	T9669	punct	",",induced
R8023	T9683	T9687	mark	while,observed
R8024	T9684	T9687	nsubjpass	it,observed
R8025	T9685	T9687	auxpass	was,observed
R8026	T9686	T9687	advmod	barely,observed
R8027	T9687	T9669	advcl	observed,induced
R8028	T9688	T9687	prep	in,observed
R8029	T9689	T9688	pobj	siRNA,in
R8030	T9690	T9691	amod	Tat-transfected,cells
R8031	T9691	T9687	dobj	cells,observed
R8032	T9692	T9687	punct	",",observed
R8033	T9693	T9694	mark	as,expected
R8034	T9694	T9687	advcl	expected,observed
R8035	T9695	T9694	prep	for,expected
R8036	T9696	T9698	det	the,transactivation
R8037	T9697	T9698	amod	Tat-dependent,transactivation
R8038	T9698	T9695	pobj	transactivation,for
R8039	T9699	T9698	prep	of,transactivation
R8040	T9700	T9702	det	the,LTR
R8041	T9701	T9702	compound	HIV-1,LTR
R8042	T9702	T9699	pobj	LTR,of
R8043	T9703	T9705	punct	(,1A
R8044	T9704	T9705	compound	Figure,1A
R8045	T9705	T9702	appos	1A,LTR
R8046	T9706	T9702	punct	),LTR
R8047	T9707	T9669	punct	.,induced
R8048	T9708	T9710	det	The,increase
R8049	T9709	T9710	amod	progressive,increase
R8050	T9710	T9741	nsubjpass	increase,observed
R8051	T9711	T9710	prep	in,increase
R8052	T9712	T9713	amod	NF-κB,activity
R8053	T9713	T9711	pobj	activity,in
R8054	T9714	T9710	punct	",",increase
R8055	T9715	T9716	mark	as,measured
R8056	T9716	T9710	advcl	measured,increase
R8057	T9717	T9716	agent	by,measured
R8058	T9718	T9717	pobj	p65,by
R8059	T9719	T9718	amod	p50,p65
R8060	T9720	T9718	amod	binding,p65
R8061	T9721	T9720	prep	to,binding
R8062	T9722	T9724	det	an,consensus
R8063	T9723	T9724	amod	NF-κB,consensus
R8064	T9724	T9721	pobj	consensus,to
R8065	T9725	T9724	appos	oligonucleotide,consensus
R8066	T9726	T9727	punct	(,Figure
R8067	T9727	T9728	nmod	Figure,1B
R8068	T9728	T9725	appos	1B,oligonucleotide
R8069	T9729	T9728	punct	),1B
R8070	T9730	T9728	cc	and,1B
R8071	T9731	T9732	amod	nuclear,p65
R8072	T9732	T9728	conj	p65,1B
R8073	T9733	T9734	punct	(,Figure
R8074	T9734	T9724	appos	Figure,consensus
R8075	T9735	T9734	nummod	1C,Figure
R8076	T9736	T9734	punct	",",Figure
R8077	T9737	T9734	appos	nucleus,Figure
R8078	T9738	T9734	punct	),Figure
R8079	T9739	T9710	punct	",",increase
R8080	T9740	T9741	auxpass	was,observed
R8081	T9741	T9775	ccomp	observed,binding
R8082	T9742	T9741	prep	at,observed
R8083	T9743	T9745	meta	1,to
R8084	T9744	T9745	punct	-,to
R8085	T9745	T9741	prep	to,observed
R8086	T9746	T9747	amod	3-h,post-infection
R8087	T9747	T9745	pobj	post-infection,to
R8088	T9748	T9747	prep	in,post-infection
R8089	T9749	T9750	det	both,Tat-positive
R8090	T9750	T9748	pobj	Tat-positive,in
R8091	T9751	T9750	punct	(,Tat-positive
R8092	T9752	T9753	det	no,siRNA
R8093	T9753	T9750	appos	siRNA,Tat-positive
R8094	T9754	T9753	cc	or,siRNA
R8095	T9755	T9756	compound	siRNA,control
R8096	T9756	T9753	conj	control,siRNA
R8097	T9757	T9753	punct	),siRNA
R8098	T9758	T9750	cc	and,Tat-positive
R8099	T9759	T9750	conj	Tat-negative,Tat-positive
R8100	T9760	T9762	punct	(,Tat
R8101	T9761	T9762	compound	siRNA,Tat
R8102	T9762	T9750	appos	Tat,Tat-positive
R8103	T9763	T9762	punct	),Tat
R8104	T9764	T9748	pobj	cells,in
R8105	T9765	T9775	punct	;,binding
R8106	T9766	T9775	advmod	however,binding
R8107	T9767	T9775	punct	",",binding
R8108	T9768	T9775	prep	at,binding
R8109	T9769	T9770	amod	12-h,post-infection
R8110	T9770	T9768	pobj	post-infection,at
R8111	T9771	T9775	punct	",",binding
R8112	T9772	T9774	det	the,DNA
R8113	T9773	T9774	nummod	p65,DNA
R8114	T9774	T9775	nsubj	DNA,binding
R8115	T9775	T9778	amod	binding,p65
R8116	T9776	T9775	cc	and,binding
R8117	T9777	T9775	conj	nuclear,binding
R8118	T9778	T9779	nsubj	p65,persisted
R8119	T9779	T9779	ROOT	persisted,persisted
R8120	T9780	T9779	conj	elevated,persisted
R8121	T9781	T9780	prep	in,elevated
R8122	T9782	T9781	pobj	presence,in
R8123	T9783	T9782	prep	of,presence
R8124	T9784	T9783	pobj	Tat,of
R8125	T9785	T9782	punct	(,presence
R8126	T9786	T9787	det	no,siRNA
R8127	T9787	T9782	appos	siRNA,presence
R8128	T9788	T9787	cc	and,siRNA
R8129	T9789	T9790	compound	siRNA,control
R8130	T9790	T9787	conj	control,siRNA
R8131	T9791	T9787	punct	),siRNA
R8132	T9792	T9780	punct	",",elevated
R8133	T9793	T9795	mark	while,dropped
R8134	T9794	T9795	nsubj	they,dropped
R8135	T9795	T9780	advcl	dropped,elevated
R8136	T9796	T9795	prep	in,dropped
R8137	T9797	T9796	pobj	absence,in
R8138	T9798	T9797	prep	of,absence
R8139	T9799	T9798	pobj	Tat,of
R8140	T9800	T9799	punct	(,Tat
R8141	T9801	T9802	compound	siRNA,Tat
R8142	T9802	T9799	appos	Tat,Tat
R8143	T9803	T9799	punct	),Tat

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