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PMC:3320587 / 13599-14748 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T6234 1147-1148 -RRB- denotes )
T6233 1141-1147 NNP denotes Pierce
T6232 1140-1141 -LRB- denotes (
T6231 1132-1139 NNP denotes Reagent
T6230 1115-1131 NNP denotes Chemiluminescent
T6229 1110-1114 NNP denotes Pico
T6228 1105-1109 NNP denotes West
T6227 1093-1104 NNP denotes SuperSignal
T6226 1088-1092 IN denotes with
T6225 1078-1087 VB denotes developed
T6224 1074-1077 CC denotes and
T6223 1069-1073 NN denotes TBST
T6222 1066-1068 IN denotes in
T6221 1062-1065 NN denotes min
T6220 1058-1061 CD denotes 3×5
T6219 1051-1057 VB denotes washed
T6218 1045-1050 RB denotes again
T6217 1041-1044 VB denotes was
T6216 1036-1040 NN denotes blot
T6215 1032-1035 DT denotes The
T6214 1029-1030 NN denotes h
T6213 1027-1028 CD denotes 1
T6212 1023-1026 IN denotes for
T6211 1011-1022 JJ denotes appropriate
T6210 1008-1010 IN denotes as
T6209 1006-1007 -RRB- denotes )
T6208 1000-1006 NN denotes BioRad
T6207 999-1000 -LRB- denotes (
T6206 983-998 NN denotes anti-rabbit-HRP
T6205 978-982 NN denotes goat
T6204 975-977 CC denotes or
T6203 973-974 -RRB- denotes )
T6202 960-973 NNP denotes Biotechnology
T6201 955-959 NNP denotes Cruz
T6200 949-954 NNP denotes Santa
T6199 948-949 -LRB- denotes (
T6198 934-947 NN denotes anti-goat-HRP
T6197 927-933 NN denotes donkey
T6196 920-926 CD denotes 1∶6000
T6195 917-919 IN denotes in
T6194 907-916 VB denotes incubated
T6193 903-906 CC denotes and
T6192 898-902 NN denotes TBST
T6191 895-897 IN denotes in
T6190 891-894 NN denotes min
T6189 887-890 CD denotes 3×5
T6188 880-886 VB denotes washed
T6187 876-879 VB denotes was
T6186 871-875 NN denotes blot
T6185 867-870 DT denotes The
T6184 854-865 NN denotes temperature
T6183 849-853 NN denotes room
T6182 846-848 IN denotes at
T6181 844-845 NN denotes h
T6180 842-843 CD denotes 2
T6179 838-841 IN denotes for
T6178 829-837 NN denotes BSA/TBST
T6177 826-828 IN denotes in
T6176 824-825 -RRB- denotes )
T6175 811-824 NNP denotes Biotechnology
T6174 806-810 NNP denotes Cruz
T6173 800-805 NNP denotes Santa
T6172 798-799 -COLON- denotes ;
T6171 791-798 NN denotes sc-6217
T6170 790-791 -LRB- denotes (
T6169 781-789 NN denotes antibody
T6168 767-780 NN denotes anti-Lamin-B1
T6167 762-766 NN denotes goat
T6166 759-761 IN denotes of
T6165 750-758 NN denotes dilution
T6164 744-749 CD denotes 1∶500
T6163 742-743 DT denotes a
T6162 738-741 CC denotes and
T6161 736-737 -RRB- denotes )
T6160 723-736 NNP denotes Biotechnology
T6159 718-722 NNP denotes Cruz
T6158 712-717 NNP denotes Santa
T6157 711-712 -LRB- denotes (
T6156 709-710 -RRB- denotes )
T6155 704-709 NN denotes H-300
T6154 703-704 -LRB- denotes (
T6153 694-702 NN denotes antibody
T6152 683-693 JJ denotes anti-NFAT5
T6151 676-682 NN denotes rabbit
T6150 673-675 IN denotes of
T6149 664-672 NN denotes dilution
T6148 657-663 NN denotes a1∶200
T6147 652-656 IN denotes with
T6146 648-651 RP denotes out
T6145 640-647 VB denotes carried
T6144 636-639 VB denotes was
T6143 625-635 NN denotes incubation
T6142 617-624 JJ denotes Primary
T6141 614-615 -RRB- denotes )
T6140 606-614 NN denotes BSA/TBST
T6139 605-606 -LRB- denotes (
T6138 601-604 CD denotes 7.6
T6137 598-600 NN denotes pH
T6136 595-597 IN denotes at
T6135 590-594 NN denotes NaCl
T6134 587-589 NN denotes mM
T6133 583-586 CD denotes 150
T6132 579-582 CC denotes and
T6131 574-578 NNP denotes Tris
T6130 571-573 NN denotes mM
T6129 568-570 CD denotes 50
T6128 557-567 VB denotes containing
T6127 550-556 NN denotes buffer
T6126 548-549 DT denotes a
T6125 545-547 IN denotes in
T6124 543-544 -RRB- denotes )
T6123 537-543 NN denotes BioRad
T6122 536-537 -LRB- denotes (
T6121 527-535 CD denotes Tween-20
T6120 525-526 NN denotes %
T6119 522-525 CD denotes 0.1
T6118 518-521 CC denotes and
T6117 516-517 -RRB- denotes )
T6116 511-516 NN denotes Sigma
T6115 510-511 -LRB- denotes (
T6114 506-509 NN denotes BSA
T6113 504-505 NN denotes %
T6104 474-475 CD denotes 1
T6103 470-473 IN denotes for
T6102 462-469 VB denotes blocked
T6101 457-461 RB denotes then
T6100 453-456 VB denotes was
T6099 448-452 NN denotes blot
T6098 444-447 DT denotes The
T6097 441-442 -RRB- denotes )
T6096 435-441 NNP denotes BioRad
T6095 434-435 -LRB- denotes (
T6094 425-433 NNP denotes Membrane
T6093 416-424 NNP denotes Transfer
T6092 405-415 NNP denotes Trans-Blot
T6091 390-404 NN denotes nitrocellulose
T6090 388-389 DT denotes a
T6089 385-387 TO denotes to
T6088 373-384 VB denotes transferred
T6087 369-372 VB denotes was
T6086 365-368 NN denotes gel
T6085 361-364 DT denotes The
T6084 351-359 NN denotes SDS-PAGE
T6083 348-350 IN denotes by
T6082 338-347 VB denotes separated
T6081 333-337 VB denotes were
T6080 324-332 NN denotes proteins
T6079 320-323 CC denotes and
T6078 302-319 NN denotes 2-mercaptoethanol
T6077 298-301 NN denotes v/v
T6076 296-297 NN denotes %
T6075 295-296 CD denotes 5
T6074 290-294 IN denotes with
T6073 283-289 NN denotes buffer
T6072 276-282 NN denotes sample
T6071 268-275 NNP denotes Laemmli
T6070 265-267 CD denotes 1×
T6069 262-264 IN denotes in
T6068 258-261 NN denotes min
T6067 256-257 CD denotes 5
T6066 252-255 IN denotes for
T6065 245-251 VB denotes boiled
T6064 240-244 VB denotes were
T6063 231-239 NN denotes Extracts
T6062 223-229 NN denotes buffer
T6061 220-222 IN denotes of
T6060 217-219 NN denotes ml
T6059 214-216 CD denotes 25
T6058 210-213 IN denotes per
T6057 208-209 -RRB- denotes )
T6056 203-208 NNP denotes Roche
T6055 202-203 -LRB- denotes (
T6054 193-201 NNP denotes Cocktail
T6053 183-192 NNP denotes Inhibitor
T6052 174-182 NNP denotes Protease
T6051 164-173 NNP denotes EDTA-free
T6050 155-163 NNP denotes Complete
T6049 152-154 IN denotes of
T6048 145-151 NN denotes tablet
T6047 143-144 CD denotes 1
T6046 139-142 CC denotes and
T6045 137-138 -COMMA- denotes ,
T6044 129-137 NN denotes glycerol
T6043 127-128 NN denotes %
T6042 125-127 CD denotes 10
T6041 123-124 -COMMA- denotes ,
T6040 117-123 NN denotes Triton
T6039 115-116 NN denotes %
T6038 114-115 CD denotes 1
T6037 112-113 -COMMA- denotes ,
T6036 109-112 CD denotes 7.5
T6035 106-108 NN denotes pH
T6034 104-105 -COMMA- denotes ,
T6033 96-104 NN denotes Tris–HCl
T6032 93-95 NN denotes mM
T6031 90-92 CD denotes 50
T6030 88-89 -COMMA- denotes ,
T6029 84-88 NN denotes NaCl
T6028 81-83 NN denotes mM
T6027 77-80 CD denotes 150
T6026 66-76 VB denotes containing
T6025 59-65 NN denotes buffer
T6024 53-58 NN denotes lysis
T6023 48-52 IN denotes with
T6022 38-47 VB denotes collected
T6021 33-37 VB denotes were
T6020 24-32 NN denotes extracts
T6019 19-23 NN denotes cell
T6018 13-18 JJ denotes Whole
T6017 8-12 NN denotes blot
T6016 0-7 NN denotes Western
T6112 503-504 CD denotes 4
T6111 500-502 IN denotes of
T6110 491-499 NN denotes solution
T6109 489-490 DT denotes a
T6108 486-488 IN denotes in
T6107 481-485 NN denotes 37°C
T6106 478-480 IN denotes at
T6105 476-477 NN denotes h
R4780 T6025 T6037 arg1Of buffer,","
R4792 T6038 T6039 arg1Of 1,%
R4793 T6040 T6038 arg1Of Triton,1
R4794 T6040 T6041 arg1Of Triton,","
R4795 T6041 T6046 arg1Of ",",and
R4796 T6043 T6042 arg1Of %,10
R4797 T6044 T6041 arg2Of glycerol,","
R4798 T6044 T6043 arg1Of glycerol,%
R4799 T6046 T6037 arg2Of and,","
R4800 T6046 T6045 arg1Of and,","
R4801 T6046 T6049 arg1Of and,of
R4802 T6048 T6046 arg2Of tablet,and
R4803 T6048 T6047 arg1Of tablet,1
R4804 T6054 T6050 arg1Of Cocktail,Complete
R4805 T6054 T6051 arg1Of Cocktail,EDTA-free
R4806 T6054 T6052 arg1Of Cocktail,Protease
R4807 T6054 T6053 arg1Of Cocktail,Inhibitor
R4808 T6054 T6055 arg1Of Cocktail,(
R4809 T6054 T6058 arg1Of Cocktail,per
R4810 T6056 T6055 arg2Of Roche,(
R4811 T6057 T6055 arg3Of ),(
R4812 T6060 T6058 arg2Of ml,per
R4813 T6060 T6059 arg1Of ml,25
R4814 T6062 T6049 arg2Of buffer,of
R4815 T6062 T6054 arg1Of buffer,Cocktail
R4816 T6062 T6061 arg1Of buffer,of
R4817 T6063 T6064 arg1Of Extracts,were
R4818 T6063 T6065 arg2Of Extracts,boiled
R4819 T6065 T6064 arg2Of boiled,were
R4820 T6065 T6066 arg1Of boiled,for
R4821 T6065 T6079 arg1Of boiled,and
R4822 T6068 T6066 arg2Of min,for
R4823 T6068 T6067 arg1Of min,5
R4824 T6068 T6069 arg1Of min,in
R4825 T6068 T6074 arg1Of min,with
R4826 T6073 T6069 arg2Of buffer,in
R4827 T6073 T6070 arg1Of buffer,1×
R4828 T6073 T6071 arg1Of buffer,Laemmli
R4829 T6073 T6072 arg1Of buffer,sample
R4830 T6075 T6076 arg1Of 5,%
R4831 T6078 T6074 arg2Of 2-mercaptoethanol,with
R4832 T6078 T6075 arg1Of 2-mercaptoethanol,5
R4833 T6078 T6077 arg1Of 2-mercaptoethanol,v/v
R4834 T6080 T6081 arg1Of proteins,were
R4835 T6080 T6082 arg2Of proteins,separated
R4836 T6082 T6079 arg2Of separated,and
R4837 T6082 T6081 arg2Of separated,were
R4838 T6084 T6082 arg1Of SDS-PAGE,separated
R4839 T6084 T6083 arg2Of SDS-PAGE,by
R4840 T6086 T6085 arg1Of gel,The
R4841 T6086 T6087 arg1Of gel,was
R4842 T6086 T6088 arg2Of gel,transferred
R4843 T6088 T6087 arg2Of transferred,was
R4844 T6088 T6089 arg1Of transferred,to
R4845 T6094 T6089 arg2Of Membrane,to
R4846 T6094 T6090 arg1Of Membrane,a
R4847 T6094 T6091 arg1Of Membrane,nitrocellulose
R4848 T6094 T6092 arg1Of Membrane,Trans-Blot
R4849 T6094 T6093 arg1Of Membrane,Transfer
R4850 T6094 T6095 arg1Of Membrane,(
R4851 T6096 T6095 arg2Of BioRad,(
R4852 T6097 T6095 arg3Of ),(
R4853 T6099 T6098 arg1Of blot,The
R4854 T6099 T6100 arg1Of blot,was
R4855 T6099 T6102 arg2Of blot,blocked
R4856 T6102 T6100 arg2Of blocked,was
R4857 T6102 T6101 arg1Of blocked,then
R4858 T6102 T6103 arg1Of blocked,for
R4859 T6102 T6106 arg1Of blocked,at
R4860 T6102 T6108 arg1Of blocked,in
R4861 T6105 T6103 arg2Of h,for
R4862 T6105 T6104 arg1Of h,1
R4863 T6107 T6106 arg2Of 37°C,at
R4864 T6110 T6108 arg2Of solution,in
R4865 T6110 T6109 arg1Of solution,a
R4866 T6110 T6111 arg1Of solution,of
R4867 T6112 T6113 arg1Of 4,%
R4868 T6114 T6112 arg1Of BSA,4
R4869 T6114 T6115 arg1Of BSA,(
R4870 T6114 T6118 arg1Of BSA,and
R4871 T6116 T6115 arg2Of Sigma,(
R4872 T6117 T6115 arg3Of ),(
R4873 T6118 T6111 arg2Of and,of
R4874 T6118 T6125 arg1Of and,in
R4875 T6120 T6118 arg2Of %,and
R4876 T6120 T6119 arg1Of %,0.1
R4877 T6120 T6121 arg1Of %,Tween-20
R4878 T6120 T6122 arg1Of %,(
R4879 T6123 T6122 arg2Of BioRad,(
R4880 T6124 T6122 arg3Of ),(
R4881 T6127 T6125 arg2Of buffer,in
R4882 T6127 T6126 arg1Of buffer,a
R4883 T6127 T6128 arg1Of buffer,containing
R4884 T6131 T6129 arg1Of Tris,50
R4885 T6131 T6130 arg1Of Tris,mM
R4886 T6131 T6132 arg1Of Tris,and
R4887 T6132 T6128 arg2Of and,containing
R4888 T6132 T6136 arg1Of and,at
R4889 T6133 T6134 arg1Of 150,mM
R4890 T6135 T6132 arg2Of NaCl,and
R4891 T6135 T6133 arg1Of NaCl,150
R4892 T6137 T6136 arg2Of pH,at
R4893 T6137 T6138 arg1Of pH,7.6
R4894 T6137 T6139 arg1Of pH,(
R4895 T6140 T6139 arg2Of BSA/TBST,(
R4896 T6141 T6139 arg3Of ),(
R4897 T6143 T6142 arg1Of incubation,Primary
R4898 T6143 T6144 arg1Of incubation,was
R4899 T6143 T6145 arg2Of incubation,carried
R4900 T6145 T6144 arg2Of carried,was
R4901 T6145 T6146 arg1Of carried,out
R4902 T6145 T6147 arg1Of carried,with
R4903 T6149 T6147 arg2Of dilution,with
R4904 T6149 T6148 arg1Of dilution,a1∶200
R4905 T6149 T6150 arg1Of dilution,of
R4906 T6153 T6151 arg1Of antibody,rabbit
R4907 T6153 T6152 arg1Of antibody,anti-NFAT5
R4908 T6153 T6154 arg1Of antibody,(
R4909 T6153 T6157 arg1Of antibody,(
R4910 T6153 T6162 arg1Of antibody,and
R4911 T6155 T6154 arg2Of H-300,(
R4912 T6156 T6154 arg3Of ),(
R4913 T6160 T6157 arg2Of Biotechnology,(
R4914 T6160 T6158 arg1Of Biotechnology,Santa
R4915 T6160 T6159 arg1Of Biotechnology,Cruz
R4916 T6161 T6157 arg3Of ),(
R4917 T6162 T6150 arg2Of and,of
R4918 T6165 T6162 arg2Of dilution,and
R4919 T6165 T6163 arg1Of dilution,a
R4920 T6165 T6164 arg1Of dilution,1∶500
R4921 T6165 T6166 arg1Of dilution,of
R4922 T6165 T6177 arg1Of dilution,in
R4923 T6169 T6166 arg2Of antibody,of
R4924 T6169 T6167 arg1Of antibody,goat
R4925 T6169 T6168 arg1Of antibody,anti-Lamin-B1
R4926 T6169 T6170 arg1Of antibody,(
R4927 T6171 T6170 arg2Of sc-6217,(
R4928 T6171 T6172 arg1Of sc-6217,;
R4929 T6175 T6172 arg2Of Biotechnology,;
R4930 T6175 T6173 arg1Of Biotechnology,Santa
R4931 T6175 T6174 arg1Of Biotechnology,Cruz
R4932 T6176 T6170 arg3Of ),(
R4933 T6178 T6177 arg2Of BSA/TBST,in
R4934 T6178 T6179 arg1Of BSA/TBST,for
R4935 T6181 T6179 arg2Of h,for
R4936 T6181 T6180 arg1Of h,2
R4937 T6181 T6182 arg1Of h,at
R4938 T6184 T6182 arg2Of temperature,at
R4939 T6184 T6183 arg1Of temperature,room
R4940 T6186 T6185 arg1Of blot,The
R4941 T6186 T6187 arg1Of blot,was
R4942 T6186 T6188 arg2Of blot,washed
R4943 T6186 T6194 arg2Of blot,incubated
R4944 T6188 T6191 arg1Of washed,in
R4945 T6188 T6193 arg1Of washed,and
R4946 T6190 T6188 arg3Of min,washed
R4947 T6190 T6189 arg1Of min,3×5
R4948 T6192 T6191 arg2Of TBST,in
R4949 T6193 T6187 arg2Of and,was
R4950 T6194 T6193 arg2Of incubated,and
R4951 T6194 T6195 arg1Of incubated,in
R4952 T6194 T6210 arg1Of incubated,as
R4953 T6198 T6196 arg1Of anti-goat-HRP,1∶6000
R4954 T6198 T6197 arg1Of anti-goat-HRP,donkey
R4955 T6198 T6199 arg1Of anti-goat-HRP,(
R4956 T6198 T6204 arg1Of anti-goat-HRP,or
R4957 T6202 T6199 arg2Of Biotechnology,(
R4958 T6202 T6200 arg1Of Biotechnology,Santa
R4959 T6202 T6201 arg1Of Biotechnology,Cruz
R4960 T6203 T6199 arg3Of ),(
R4961 T6204 T6195 arg2Of or,in
R4962 T6206 T6204 arg2Of anti-rabbit-HRP,or
R4963 T6206 T6205 arg1Of anti-rabbit-HRP,goat
R4964 T6206 T6207 arg1Of anti-rabbit-HRP,(
R4965 T6208 T6207 arg2Of BioRad,(
R4966 T6209 T6207 arg3Of ),(
R4967 T6211 T6210 arg2Of appropriate,as
R4968 T6211 T6212 arg1Of appropriate,for
R4969 T6214 T6212 arg2Of h,for
R4970 T6214 T6213 arg1Of h,1
R4971 T6216 T6215 arg1Of blot,The
R4972 T6216 T6217 arg1Of blot,was
R4973 T6216 T6219 arg2Of blot,washed
R4974 T6216 T6225 arg1Of blot,developed
R4975 T6219 T6217 arg2Of washed,was
R4976 T6219 T6218 arg1Of washed,again
R4977 T6219 T6222 arg1Of washed,in
R4978 T6219 T6224 arg1Of washed,and
R4979 T6221 T6219 arg3Of min,washed
R4980 T6221 T6220 arg1Of min,3×5
R4981 T6223 T6222 arg2Of TBST,in
R4982 T6225 T6224 arg2Of developed,and
R4983 T6225 T6226 arg1Of developed,with
R4984 T6228 T6226 arg2Of West,with
R4985 T6228 T6227 arg1Of West,SuperSignal
R4986 T6231 T6225 arg2Of Reagent,developed
R4987 T6231 T6229 arg1Of Reagent,Pico
R4988 T6231 T6230 arg1Of Reagent,Chemiluminescent
R4989 T6231 T6232 arg1Of Reagent,(
R4990 T6233 T6232 arg2Of Pierce,(
R4991 T6234 T6232 arg3Of ),(
R4771 T6017 T6016 arg1Of blot,Western
R4772 T6020 T6018 arg1Of extracts,Whole
R4773 T6020 T6019 arg1Of extracts,cell
R4774 T6020 T6021 arg1Of extracts,were
R4775 T6020 T6022 arg2Of extracts,collected
R4776 T6022 T6021 arg2Of collected,were
R4777 T6022 T6023 arg1Of collected,with
R4778 T6025 T6024 arg1Of buffer,lysis
R4779 T6025 T6026 arg1Of buffer,containing
R4781 T6027 T6028 arg1Of 150,mM
R4782 T6029 T6027 arg1Of NaCl,150
R4783 T6029 T6030 arg1Of NaCl,","
R4784 T6030 T6034 arg1Of ",",","
R4785 T6031 T6032 arg1Of 50,mM
R4786 T6033 T6030 arg2Of Tris–HCl,","
R4787 T6033 T6031 arg1Of Tris–HCl,50
R4788 T6034 T6026 arg2Of ",",containing
R4789 T6035 T6034 arg2Of pH,","
R4790 T6035 T6036 arg1Of pH,7.5
R4791 T6037 T6023 arg2Of ",",with

bionlp-st-ge-2016-test-proteins

Id Subject Object Predicate Lexical cue
T6004 829-832 Protein denotes BSA
T6003 506-509 Protein denotes BSA

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T6235 688-693 http://www.uniprot.org/uniprot/O94916 denotes NFAT5

GO-BP

Id Subject Object Predicate Lexical cue
T6005 53-58 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis

GO-MF

Id Subject Object Predicate Lexical cue
T6007 781-789 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T6006 694-702 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody

GO-CC

Id Subject Object Predicate Lexical cue
T6013 781-789 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T6012 694-702 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T6011 781-789 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T6010 694-702 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T6009 425-433 http://purl.obolibrary.org/obo/GO_0016020 denotes Membrane
T6008 19-23 http://purl.obolibrary.org/obo/GO_0005623 denotes cell

sentences

Id Subject Object Predicate Lexical cue
T6000 1032-1149 Sentence denotes The blot was again washed 3×5 min in TBST and developed with SuperSignal West Pico Chemiluminescent Reagent (Pierce).
T5999 867-1031 Sentence denotes The blot was washed 3×5 min in TBST and incubated in 1∶6000 donkey anti-goat-HRP (Santa Cruz Biotechnology) or goat anti-rabbit-HRP (BioRad) as appropriate for 1 h.
T5998 617-866 Sentence denotes Primary incubation was carried out with a1∶200 dilution of rabbit anti-NFAT5 antibody (H-300) (Santa Cruz Biotechnology) and a 1∶500 dilution of goat anti-Lamin-B1 antibody (sc-6217; Santa Cruz Biotechnology) in BSA/TBST for 2 h at room temperature.
T5997 444-616 Sentence denotes The blot was then blocked for 1 h at 37°C in a solution of 4% BSA (Sigma) and 0.1% Tween-20 (BioRad) in a buffer containing 50 mM Tris and 150 mM NaCl at pH 7.6 (BSA/TBST).
T5996 361-443 Sentence denotes The gel was transferred to a nitrocellulose Trans-Blot Transfer Membrane (BioRad).
T5995 231-360 Sentence denotes Extracts were boiled for 5 min in 1× Laemmli sample buffer with 5% v/v 2-mercaptoethanol and proteins were separated by SDS-PAGE.
T5994 13-230 Sentence denotes Whole cell extracts were collected with lysis buffer containing 150 mM NaCl, 50 mM Tris–HCl, pH 7.5, 1% Triton, 10% glycerol, and 1 tablet of Complete EDTA-free Protease Inhibitor Cocktail (Roche) per 25 ml of buffer.
T5993 0-12 Sentence denotes Western blot
T83 0-12 Sentence denotes Western blot
T84 13-230 Sentence denotes Whole cell extracts were collected with lysis buffer containing 150 mM NaCl, 50 mM Tris–HCl, pH 7.5, 1% Triton, 10% glycerol, and 1 tablet of Complete EDTA-free Protease Inhibitor Cocktail (Roche) per 25 ml of buffer.
T85 231-360 Sentence denotes Extracts were boiled for 5 min in 1× Laemmli sample buffer with 5% v/v 2-mercaptoethanol and proteins were separated by SDS-PAGE.
T86 361-443 Sentence denotes The gel was transferred to a nitrocellulose Trans-Blot Transfer Membrane (BioRad).
T87 444-616 Sentence denotes The blot was then blocked for 1 h at 37°C in a solution of 4% BSA (Sigma) and 0.1% Tween-20 (BioRad) in a buffer containing 50 mM Tris and 150 mM NaCl at pH 7.6 (BSA/TBST).
T88 617-866 Sentence denotes Primary incubation was carried out with a1∶200 dilution of rabbit anti-NFAT5 antibody (H-300) (Santa Cruz Biotechnology) and a 1∶500 dilution of goat anti-Lamin-B1 antibody (sc-6217; Santa Cruz Biotechnology) in BSA/TBST for 2 h at room temperature.
T89 867-1031 Sentence denotes The blot was washed 3×5 min in TBST and incubated in 1∶6000 donkey anti-goat-HRP (Santa Cruz Biotechnology) or goat anti-rabbit-HRP (BioRad) as appropriate for 1 h.
T90 1032-1149 Sentence denotes The blot was again washed 3×5 min in TBST and developed with SuperSignal West Pico Chemiluminescent Reagent (Pierce).

simple1

Id Subject Object Predicate Lexical cue
T6015 829-832 Protein denotes BSA
T6014 506-509 Protein denotes BSA

BioNLP16_DUT

Id Subject Object Predicate Lexical cue
T6489 829-832 Protein denotes BSA
T6488 506-509 Protein denotes BSA

BioNLP16_Messiy

Id Subject Object Predicate Lexical cue
T6243 829-832 Protein denotes BSA
T6242 506-509 Protein denotes BSA

DLUT931

Id Subject Object Predicate Lexical cue
T6241 829-832 Protein denotes BSA
T6240 506-509 Protein denotes BSA

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T6520 1093-1148 Entity denotes SuperSignal West Pico Chemiluminescent Reagent (Pierce)
T6519 489-499 Entity denotes a solution
T6518 759-789 Protein denotes of goat anti-Lamin-B1 antibody
T6517 842-865 Entity denotes 2 h at room temperature
T6516 231-239 Entity denotes Extracts
T6515 673-710 Entity denotes of rabbit anti-NFAT5 antibody (H-300)
T6514 934-974 Protein denotes anti-goat-HRP (Santa Cruz Biotechnology)
T6513 96-104 Entity denotes Tris–HCl
T6512 77-105 Entity denotes 150 mM NaCl, 50 mM Tris–HCl,
T6511 388-442 Entity denotes a nitrocellulose Trans-Blot Transfer Membrane (BioRad)
T6510 361-368 Entity denotes The gel
T6509 164-173 Entity denotes EDTA-free
T6508 712-717 Protein denotes Santa
T6507 568-578 Entity denotes 50 mM Tris
T6506 96-104 Entity denotes Tris–HCl
T6505 324-332 Protein denotes proteins
T6504 114-123 Entity denotes 1% Triton
T6503 583-594 Entity denotes 150 mM NaCl
T6502 298-319 Entity denotes v/v 2-mercaptoethanol
T6501 474-477 Entity denotes 1 h
T6500 155-182 Protein denotes Complete EDTA-free Protease
T6499 503-517 Protein denotes 4% BSA (Sigma)
T6498 800-805 Protein denotes Santa
T6497 101-104 Entity denotes HCl
T6496 511-516 Protein denotes Sigma
T6495 125-137 Entity denotes 10% glycerol
T6494 24-32 Entity denotes extracts
T6493 183-192 Entity denotes Inhibitor
T6492 388-442 Entity denotes a nitrocellulose Trans-Blot Transfer Membrane (BioRad)
T6491 606-609 Protein denotes BSA
T6490 405-410 Protein denotes Trans

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T6485 1148-1149 . denotes .
T6484 1147-1148 -RRB- denotes )
T6483 1141-1147 NNP denotes Pierce
T6482 1140-1141 -LRB- denotes (
T6481 1132-1139 NNP denotes Reagent
T6480 1115-1131 NNP denotes Chemiluminescent
T6479 1110-1114 NNP denotes Pico
T6478 1105-1109 NNP denotes West
T6477 1093-1104 NNP denotes SuperSignal
T6476 1088-1092 IN denotes with
T6475 1078-1087 VBN denotes developed
T6474 1074-1077 CC denotes and
T6473 1069-1073 NNP denotes TBST
T6472 1066-1068 IN denotes in
T6471 1062-1065 NN denotes min
T6470 1060-1061 CD denotes 5
T6469 1059-1060 NN denotes ×
T6468 1058-1059 CD denotes 3
T6467 1051-1057 VBN denotes washed
T6466 1045-1050 RB denotes again
T6465 1041-1044 VBD denotes was
T6464 1036-1040 NN denotes blot
T6463 1032-1035 DT denotes The
T6462 1030-1031 . denotes .
T6461 1029-1030 NN denotes h
T6460 1027-1028 CD denotes 1
T6459 1023-1026 IN denotes for
T6458 1011-1022 JJ denotes appropriate
T6457 1008-1010 IN denotes as
T6456 1006-1007 -RRB- denotes )
T6455 1000-1006 NNP denotes BioRad
T6454 999-1000 -LRB- denotes (
T6453 983-998 JJ denotes anti-rabbit-HRP
T6452 978-982 VB denotes goat
T6451 975-977 CC denotes or
T6450 973-974 -RRB- denotes )
T6449 960-973 NNP denotes Biotechnology
T6448 955-959 NNP denotes Cruz
T6447 949-954 NNP denotes Santa
T6446 948-949 -LRB- denotes (
T6445 934-947 JJ denotes anti-goat-HRP
T6444 927-933 NN denotes donkey
T6443 922-926 CD denotes 6000
T6442 921-922 CD denotes ∶
T6441 920-921 CD denotes 1
T6440 917-919 IN denotes in
T6439 907-916 VBN denotes incubated
T6438 903-906 CC denotes and
T6437 898-902 NNP denotes TBST
T6436 895-897 IN denotes in
T6435 891-894 NN denotes min
T6434 889-890 CD denotes 5
T6433 888-889 NN denotes ×
T6432 887-888 CD denotes 3
T6431 880-886 VBN denotes washed
T6430 876-879 VBD denotes was
T6429 871-875 NN denotes blot
T6428 867-870 DT denotes The
T6427 865-866 . denotes .
T6426 854-865 NN denotes temperature
T6425 849-853 NN denotes room
T6424 846-848 IN denotes at
T6423 844-845 NN denotes h
T6422 842-843 CD denotes 2
T6421 838-841 IN denotes for
T6420 829-837 NNP denotes BSA/TBST
T6419 826-828 IN denotes in
T6418 824-825 -RRB- denotes )
T6417 811-824 NNP denotes Biotechnology
T6416 806-810 NNP denotes Cruz
T6415 800-805 NNP denotes Santa
T6414 798-799 : denotes ;
T6413 791-798 JJ denotes sc-6217
T6412 790-791 -LRB- denotes (
T6411 781-789 NN denotes antibody
T6410 767-780 JJ denotes anti-Lamin-B1
T6409 762-766 NN denotes goat
T6408 759-761 IN denotes of
T6407 750-758 NN denotes dilution
T6406 746-749 CD denotes 500
T6405 745-746 NN denotes ∶
T6404 744-745 CD denotes 1
T6403 742-743 DT denotes a
T6402 738-741 CC denotes and
T6401 736-737 -RRB- denotes )
T6400 723-736 NNP denotes Biotechnology
T6399 718-722 NNP denotes Cruz
T6398 712-717 NNP denotes Santa
T6397 711-712 -LRB- denotes (
T6396 709-710 -RRB- denotes )
T6395 704-709 NN denotes H-300
T6394 703-704 -LRB- denotes (
T6393 694-702 NN denotes antibody
T6392 683-693 JJ denotes anti-NFAT5
T6391 676-682 NN denotes rabbit
T6390 673-675 IN denotes of
T6389 664-672 NN denotes dilution
T6388 660-663 CD denotes 200
T6387 659-660 CD denotes ∶
T6386 657-659 CD denotes a1
T6385 652-656 IN denotes with
T6384 648-651 RP denotes out
T6383 640-647 VBN denotes carried
T6382 636-639 VBD denotes was
T6381 625-635 NN denotes incubation
T6380 617-624 JJ denotes Primary
T6379 615-616 . denotes .
T6378 614-615 -RRB- denotes )
T6377 606-614 NNP denotes BSA/TBST
T6376 605-606 -LRB- denotes (
T6375 601-604 CD denotes 7.6
T6374 598-600 NNP denotes pH
T6373 595-597 IN denotes at
T6372 590-594 NNP denotes NaCl
T6371 587-589 NNP denotes mM
T6370 583-586 CD denotes 150
T6369 579-582 CC denotes and
T6368 574-578 NNP denotes Tris
T6367 571-573 NNP denotes mM
T6366 568-570 CD denotes 50
T6365 557-567 VBG denotes containing
T6364 550-556 NN denotes buffer
T6363 548-549 DT denotes a
T6362 545-547 IN denotes in
T6361 543-544 -RRB- denotes )
T6360 537-543 NNP denotes BioRad
T6359 536-537 -LRB- denotes (
T6358 527-535 NN denotes Tween-20
T6357 525-526 NN denotes %
T6356 522-525 CD denotes 0.1
T6355 518-521 CC denotes and
T6354 516-517 -RRB- denotes )
T6353 511-516 NNP denotes Sigma
T6352 510-511 -LRB- denotes (
T6351 506-509 NNP denotes BSA
T6350 504-505 NN denotes %
T6349 503-504 CD denotes 4
T6348 500-502 IN denotes of
T6347 491-499 NN denotes solution
T6346 489-490 DT denotes a
T6345 486-488 IN denotes in
T6344 484-485 NNP denotes C
T6343 483-484 CD denotes °
T6342 481-483 CD denotes 37
T6341 478-480 IN denotes at
T6340 476-477 NN denotes h
T6339 474-475 CD denotes 1
T6338 470-473 IN denotes for
T6337 462-469 VBN denotes blocked
T6336 457-461 RB denotes then
T6335 453-456 VBD denotes was
T6334 448-452 NN denotes blot
T6333 444-447 DT denotes The
T6332 442-443 . denotes .
T6331 441-442 -RRB- denotes )
T6328 425-433 NNP denotes Membrane
T6327 416-424 NNP denotes Transfer
T6326 405-415 NNP denotes Trans-Blot
T6325 390-404 JJ denotes nitrocellulose
T6324 388-389 DT denotes a
T6323 385-387 TO denotes to
T6322 373-384 VBN denotes transferred
T6321 369-372 VBD denotes was
T6320 365-368 NN denotes gel
T6319 361-364 DT denotes The
T6318 359-360 . denotes .
T6317 351-359 NNP denotes SDS-PAGE
T6316 348-350 IN denotes by
T6315 338-347 VBN denotes separated
T6314 333-337 VBD denotes were
T6313 324-332 NNS denotes proteins
T6312 320-323 CC denotes and
T6311 302-319 JJ denotes 2-mercaptoethanol
T6310 298-301 NN denotes v/v
T6309 296-297 NN denotes %
T6308 295-296 CD denotes 5
T6307 290-294 IN denotes with
T6306 283-289 NN denotes buffer
T6305 276-282 NN denotes sample
T6304 268-275 NNP denotes Laemmli
T6303 266-267 CD denotes ×
T6302 265-266 CD denotes 1
T6301 262-264 IN denotes in
T6300 258-261 NN denotes min
T6299 256-257 CD denotes 5
T6298 252-255 IN denotes for
T6297 245-251 VBN denotes boiled
T6296 240-244 VBD denotes were
T6295 231-239 NNS denotes Extracts
T6294 229-230 . denotes .
T6293 223-229 NN denotes buffer
T6292 220-222 IN denotes of
T6291 217-219 NN denotes ml
T6290 214-216 CD denotes 25
T6289 210-213 IN denotes per
T6288 208-209 -RRB- denotes )
T6287 203-208 NNP denotes Roche
T6286 202-203 -LRB- denotes (
T6285 193-201 NNP denotes Cocktail
T6284 183-192 NNP denotes Inhibitor
T6283 174-182 NNP denotes Protease
T6282 164-173 JJ denotes EDTA-free
T6281 155-163 JJ denotes Complete
T6280 152-154 IN denotes of
T6279 145-151 NN denotes tablet
T6278 143-144 CD denotes 1
T6277 139-142 CC denotes and
T6276 137-138 , denotes ,
T6275 129-137 NN denotes glycerol
T6274 127-128 NN denotes %
T6273 125-127 CD denotes 10
T6272 123-124 , denotes ,
T6271 117-123 NNP denotes Triton
T6270 115-116 NN denotes %
T6269 114-115 CD denotes 1
T6268 112-113 , denotes ,
T6267 109-112 CD denotes 7.5
T6266 106-108 NNP denotes pH
T6265 104-105 , denotes ,
T6264 101-104 NNP denotes HCl
T6263 96-100 NNP denotes Tris
T6262 93-95 NNP denotes mM
T6261 90-92 CD denotes 50
T6260 88-89 , denotes ,
T6259 84-88 NNP denotes NaCl
T6258 81-83 NNP denotes mM
T6257 77-80 CD denotes 150
T6256 66-76 VBG denotes containing
T6255 59-65 NN denotes buffer
T6254 53-58 NN denotes lysis
T6253 48-52 IN denotes with
T6252 38-47 VBN denotes collected
T6251 33-37 VBD denotes were
T6250 24-32 NNS denotes extracts
T6330 435-441 NNP denotes BioRad
T6329 434-435 -LRB- denotes (
T6249 19-23 NN denotes cell
T6248 13-18 NNP denotes Whole
T6247 8-12 NN denotes blot
T6246 0-7 JJ denotes Western
R4992 T6246 T6250 amod Western,extracts
R4993 T6247 T6250 amod blot,extracts
R4994 T6248 T6250 amod Whole,extracts
R4995 T6249 T6250 compound cell,extracts
R4996 T6250 T6252 nsubjpass extracts,collected
R4997 T6251 T6252 auxpass were,collected
R4998 T6252 T6252 ROOT collected,collected
R4999 T6253 T6252 prep with,collected
R5000 T6254 T6255 compound lysis,buffer
R5001 T6255 T6253 pobj buffer,with
R5002 T6256 T6255 acl containing,buffer
R5064 T6318 T6297 punct .,boiled
R5065 T6319 T6320 det The,gel
R5066 T6320 T6322 nsubjpass gel,transferred
R5067 T6321 T6322 auxpass was,transferred
R5068 T6322 T6322 ROOT transferred,transferred
R5069 T6323 T6322 prep to,transferred
R5070 T6324 T6328 det a,Membrane
R5071 T6325 T6328 amod nitrocellulose,Membrane
R5072 T6326 T6328 compound Trans-Blot,Membrane
R5073 T6327 T6328 compound Transfer,Membrane
R5074 T6328 T6323 pobj Membrane,to
R5075 T6329 T6328 punct (,Membrane
R5076 T6330 T6328 appos BioRad,Membrane
R5077 T6331 T6328 punct ),Membrane
R5078 T6332 T6322 punct .,transferred
R5079 T6333 T6334 det The,blot
R5080 T6334 T6337 nsubjpass blot,blocked
R5081 T6335 T6337 auxpass was,blocked
R5082 T6336 T6337 advmod then,blocked
R5083 T6337 T6337 ROOT blocked,blocked
R5084 T6338 T6337 prep for,blocked
R5085 T6339 T6340 nummod 1,h
R5086 T6340 T6338 pobj h,for
R5087 T6341 T6337 prep at,blocked
R5088 T6342 T6343 nummod 37,°
R5089 T6343 T6344 nummod °,C
R5090 T6344 T6341 pobj C,at
R5091 T6345 T6337 prep in,blocked
R5092 T6346 T6347 det a,solution
R5093 T6347 T6345 pobj solution,in
R5094 T6348 T6347 prep of,solution
R5095 T6349 T6350 nummod 4,%
R5096 T6350 T6348 pobj %,of
R5097 T6351 T6348 pobj BSA,of
R5098 T6352 T6353 punct (,Sigma
R5099 T6353 T6351 appos Sigma,BSA
R5100 T6354 T6353 punct ),Sigma
R5101 T6355 T6351 cc and,BSA
R5102 T6356 T6357 nummod 0.1,%
R5103 T6357 T6358 compound %,Tween-20
R5104 T6358 T6351 conj Tween-20,BSA
R5105 T6359 T6360 punct (,BioRad
R5106 T6360 T6358 appos BioRad,Tween-20
R5107 T6361 T6360 punct ),BioRad
R5108 T6362 T6347 prep in,solution
R5109 T6363 T6364 det a,buffer
R5110 T6364 T6362 pobj buffer,in
R5111 T6365 T6364 acl containing,buffer
R5112 T6366 T6368 nummod 50,Tris
R5113 T6367 T6368 compound mM,Tris
R5114 T6368 T6365 dobj Tris,containing
R5115 T6369 T6368 cc and,Tris
R5116 T6370 T6372 nummod 150,NaCl
R5117 T6371 T6372 compound mM,NaCl
R5118 T6372 T6368 conj NaCl,Tris
R5119 T6373 T6365 prep at,containing
R5120 T6374 T6373 pobj pH,at
R5121 T6375 T6374 nummod 7.6,pH
R5122 T6376 T6377 punct (,BSA/TBST
R5123 T6377 T6374 appos BSA/TBST,pH
R5124 T6378 T6374 punct ),pH
R5125 T6379 T6337 punct .,blocked
R5126 T6380 T6381 amod Primary,incubation
R5127 T6381 T6383 nsubjpass incubation,carried
R5128 T6382 T6383 auxpass was,carried
R5129 T6383 T6383 ROOT carried,carried
R5130 T6384 T6383 prt out,carried
R5131 T6385 T6383 prep with,carried
R5132 T6386 T6389 nummod a1,dilution
R5133 T6387 T6389 nummod ∶,dilution
R5134 T6388 T6389 nummod 200,dilution
R5135 T6389 T6385 pobj dilution,with
R5136 T6390 T6389 prep of,dilution
R5137 T6391 T6393 nmod rabbit,antibody
R5138 T6392 T6393 amod anti-NFAT5,antibody
R5139 T6393 T6390 pobj antibody,of
R5140 T6394 T6393 punct (,antibody
R5141 T6395 T6393 appos H-300,antibody
R5142 T6396 T6393 punct ),antibody
R5143 T6397 T6400 punct (,Biotechnology
R5144 T6398 T6399 compound Santa,Cruz
R5145 T6399 T6400 compound Cruz,Biotechnology
R5146 T6400 T6389 appos Biotechnology,dilution
R5147 T6401 T6400 punct ),Biotechnology
R5148 T6402 T6389 cc and,dilution
R5149 T6403 T6407 det a,dilution
R5150 T6404 T6407 nummod 1,dilution
R5151 T6405 T6407 nmod ∶,dilution
R5152 T6406 T6407 nummod 500,dilution
R5153 T6407 T6389 conj dilution,dilution
R5154 T6408 T6407 prep of,dilution
R5155 T6409 T6411 nmod goat,antibody
R5156 T6410 T6411 amod anti-Lamin-B1,antibody
R5157 T6411 T6408 pobj antibody,of
R5158 T6412 T6413 punct (,sc-6217
R5160 T6414 T6407 punct ;,dilution
R5161 T6415 T6416 compound Santa,Cruz
R5162 T6416 T6417 compound Cruz,Biotechnology
R5163 T6417 T6407 appos Biotechnology,dilution
R5164 T6418 T6407 punct ),dilution
R5165 T6419 T6407 prep in,dilution
R5166 T6420 T6419 pobj BSA/TBST,in
R5167 T6421 T6407 prep for,dilution
R5168 T6422 T6423 nummod 2,h
R5169 T6423 T6421 pobj h,for
R5170 T6424 T6423 prep at,h
R5171 T6425 T6426 compound room,temperature
R5172 T6426 T6424 pobj temperature,at
R5173 T6427 T6383 punct .,carried
R5174 T6428 T6429 det The,blot
R5175 T6429 T6431 nsubjpass blot,washed
R5176 T6430 T6431 auxpass was,washed
R5177 T6431 T6431 ROOT washed,washed
R5178 T6432 T6433 nummod 3,×
R5179 T6433 T6431 npadvmod ×,washed
R5180 T6434 T6435 nummod 5,min
R5181 T6435 T6433 appos min,×
R5182 T6436 T6435 prep in,min
R5183 T6437 T6436 pobj TBST,in
R5184 T6438 T6435 cc and,min
R5185 T6439 T6435 conj incubated,min
R5186 T6440 T6439 prep in,incubated
R5187 T6441 T6445 nummod 1,anti-goat-HRP
R5188 T6442 T6445 nummod ∶,anti-goat-HRP
R5189 T6443 T6445 nummod 6000,anti-goat-HRP
R5190 T6444 T6445 compound donkey,anti-goat-HRP
R5191 T6445 T6440 pobj anti-goat-HRP,in
R5192 T6446 T6449 punct (,Biotechnology
R5193 T6447 T6449 compound Santa,Biotechnology
R5194 T6448 T6449 compound Cruz,Biotechnology
R5195 T6449 T6445 appos Biotechnology,anti-goat-HRP
R5196 T6450 T6449 punct ),Biotechnology
R5197 T6451 T6445 cc or,anti-goat-HRP
R5198 T6452 T6453 compound goat,anti-rabbit-HRP
R5199 T6453 T6445 conj anti-rabbit-HRP,anti-goat-HRP
R5200 T6454 T6455 punct (,BioRad
R5201 T6455 T6453 appos BioRad,anti-rabbit-HRP
R5202 T6456 T6453 punct ),anti-rabbit-HRP
R5203 T6457 T6445 prep as,anti-goat-HRP
R5204 T6458 T6457 amod appropriate,as
R5205 T6459 T6439 prep for,incubated
R5206 T6460 T6461 nummod 1,h
R5207 T6461 T6459 pobj h,for
R5208 T6462 T6431 punct .,washed
R5209 T6463 T6464 det The,blot
R5210 T6464 T6467 nsubjpass blot,washed
R5211 T6465 T6467 auxpass was,washed
R5212 T6466 T6467 advmod again,washed
R5213 T6467 T6467 ROOT washed,washed
R5214 T6468 T6471 nummod 3,min
R5215 T6469 T6471 nmod ×,min
R5216 T6470 T6469 nummod 5,×
R5217 T6471 T6467 dobj min,washed
R5218 T6472 T6471 prep in,min
R5219 T6473 T6472 pobj TBST,in
R5220 T6474 T6467 cc and,washed
R5221 T6475 T6467 conj developed,washed
R5222 T6476 T6475 prep with,developed
R5223 T6477 T6481 compound SuperSignal,Reagent
R5224 T6478 T6479 compound West,Pico
R5225 T6479 T6481 compound Pico,Reagent
R5226 T6480 T6481 compound Chemiluminescent,Reagent
R5227 T6481 T6476 pobj Reagent,with
R5228 T6482 T6483 punct (,Pierce
R5229 T6483 T6481 appos Pierce,Reagent
R5230 T6484 T6481 punct ),Reagent
R5231 T6485 T6467 punct .,washed
R5003 T6257 T6259 nummod 150,NaCl
R5004 T6258 T6259 compound mM,NaCl
R5005 T6259 T6256 dobj NaCl,containing
R5006 T6260 T6259 punct ",",NaCl
R5007 T6261 T6263 nummod 50,Tris
R5008 T6262 T6263 compound mM,Tris
R5009 T6263 T6264 compound Tris,HCl
R5010 T6264 T6259 appos HCl,NaCl
R5011 T6265 T6264 punct ",",HCl
R5012 T6266 T6264 conj pH,HCl
R5013 T6267 T6266 nummod 7.5,pH
R5014 T6268 T6266 punct ",",pH
R5015 T6269 T6270 nummod 1,%
R5016 T6270 T6271 compound %,Triton
R5017 T6271 T6266 appos Triton,pH
R5018 T6272 T6271 punct ",",Triton
R5019 T6273 T6274 nummod 10,%
R5020 T6274 T6275 compound %,glycerol
R5021 T6275 T6271 conj glycerol,Triton
R5022 T6276 T6275 punct ",",glycerol
R5023 T6277 T6275 cc and,glycerol
R5024 T6278 T6279 nummod 1,tablet
R5025 T6279 T6275 conj tablet,glycerol
R5026 T6280 T6279 prep of,tablet
R5027 T6281 T6282 amod Complete,EDTA-free
R5028 T6282 T6285 amod EDTA-free,Cocktail
R5029 T6283 T6285 compound Protease,Cocktail
R5030 T6284 T6285 compound Inhibitor,Cocktail
R5031 T6285 T6280 pobj Cocktail,of
R5032 T6286 T6287 punct (,Roche
R5159 T6413 T6407 dep sc-6217,dilution
R5033 T6287 T6285 appos Roche,Cocktail
R5034 T6288 T6285 punct ),Cocktail
R5035 T6289 T6279 prep per,tablet
R5036 T6290 T6291 nummod 25,ml
R5037 T6291 T6289 pobj ml,per
R5038 T6292 T6291 prep of,ml
R5039 T6293 T6292 pobj buffer,of
R5040 T6294 T6252 punct .,collected
R5041 T6295 T6297 nsubjpass Extracts,boiled
R5042 T6296 T6297 auxpass were,boiled
R5043 T6297 T6297 ROOT boiled,boiled
R5044 T6298 T6297 prep for,boiled
R5045 T6299 T6300 nummod 5,min
R5046 T6300 T6298 pobj min,for
R5047 T6301 T6297 prep in,boiled
R5048 T6302 T6303 nummod 1,×
R5049 T6303 T6301 pobj ×,in
R5050 T6304 T6306 compound Laemmli,buffer
R5051 T6305 T6306 compound sample,buffer
R5052 T6306 T6297 advcl buffer,boiled
R5053 T6307 T6306 prep with,buffer
R5054 T6308 T6309 nummod 5,%
R5055 T6309 T6311 nmod %,2-mercaptoethanol
R5056 T6310 T6311 compound v/v,2-mercaptoethanol
R5057 T6311 T6307 pobj 2-mercaptoethanol,with
R5058 T6312 T6311 cc and,2-mercaptoethanol
R5059 T6313 T6315 nsubjpass proteins,separated
R5060 T6314 T6315 auxpass were,separated
R5061 T6315 T6311 conj separated,2-mercaptoethanol
R5062 T6316 T6315 agent by,separated
R5063 T6317 T6316 pobj SDS-PAGE,by

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T6245 688-693 Protein denotes NFAT5
T6244 174-192 Protein denotes Protease Inhibitor

testone

Id Subject Object Predicate Lexical cue
T5988 829-832 Protein denotes BSA
T5987 506-509 Protein denotes BSA

test3

Id Subject Object Predicate Lexical cue
T5992 829-832 Protein denotes BSA
T5991 506-509 Protein denotes BSA
T5990 829-832 Protein denotes BSA
T5989 506-509 Protein denotes BSA