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pmc-enju-pas

bionlp-st-ge-2016-test-proteins

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T4664","span":{"begin":746,"end":751},"obj":"Protein"},{"id":"T4663","span":{"begin":270,"end":273},"obj":"Protein"},{"id":"T4662","span":{"begin":243,"end":268},"obj":"Protein"},{"id":"T4661","span":{"begin":90,"end":95},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"An siRNA was constructed (Ambion Inc., www.ambion.com) to target a sequence unique to the NFAT5 transcript: 5′-CAACATGCCTGGAATTCAA-3′ (nt 335 to 353) [31]. As described, a control for non-specific siRNA effects, we used an siRNA targeting the green fluorescent protein (GFP), 5′- GGCTACGTCCAGGAGCGCACC-3′. MDM were transfected in 6-well plates using 1 µM of the indicated siRNA in siPORT NeoFX transfection reagent (Ambion Inc., www.ambion.com), prepared as recommended by the manufacturer, in a final volume of 750 µl in Macrophage-SFM medium plus 5% heat-inactivated human AB serum. The cultures were left at 37°C overnight after which cells were washed and incubated in fresh medium. MDM were transfected two times for efficient knock down of NFAT5 expression before infection experiments were performed [31]."}

bionlp-st-ge-2016-uniprot

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T4817","span":{"begin":270,"end":273},"obj":"http://www.uniprot.org/uniprot/Q9U6Y4"},{"id":"T4816","span":{"begin":746,"end":751},"obj":"http://www.uniprot.org/uniprot/O94916"},{"id":"T4815","span":{"begin":90,"end":95},"obj":"http://www.uniprot.org/uniprot/O94916"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"An siRNA was constructed (Ambion Inc., www.ambion.com) to target a sequence unique to the NFAT5 transcript: 5′-CAACATGCCTGGAATTCAA-3′ (nt 335 to 353) [31]. As described, a control for non-specific siRNA effects, we used an siRNA targeting the green fluorescent protein (GFP), 5′- GGCTACGTCCAGGAGCGCACC-3′. MDM were transfected in 6-well plates using 1 µM of the indicated siRNA in siPORT NeoFX transfection reagent (Ambion Inc., www.ambion.com), prepared as recommended by the manufacturer, in a final volume of 750 µl in Macrophage-SFM medium plus 5% heat-inactivated human AB serum. The cultures were left at 37°C overnight after which cells were washed and incubated in fresh medium. MDM were transfected two times for efficient knock down of NFAT5 expression before infection experiments were performed [31]."}

UBERON-AE

{"project":"UBERON-AE","denotations":[{"id":"T4645","span":{"begin":578,"end":583},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"text":"An siRNA was constructed (Ambion Inc., www.ambion.com) to target a sequence unique to the NFAT5 transcript: 5′-CAACATGCCTGGAATTCAA-3′ (nt 335 to 353) [31]. As described, a control for non-specific siRNA effects, we used an siRNA targeting the green fluorescent protein (GFP), 5′- GGCTACGTCCAGGAGCGCACC-3′. MDM were transfected in 6-well plates using 1 µM of the indicated siRNA in siPORT NeoFX transfection reagent (Ambion Inc., www.ambion.com), prepared as recommended by the manufacturer, in a final volume of 750 µl in Macrophage-SFM medium plus 5% heat-inactivated human AB serum. The cultures were left at 37°C overnight after which cells were washed and incubated in fresh medium. MDM were transfected two times for efficient knock down of NFAT5 expression before infection experiments were performed [31]."}

GO-CC

{"project":"GO-CC","denotations":[{"id":"T4665","span":{"begin":638,"end":643},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"An siRNA was constructed (Ambion Inc., www.ambion.com) to target a sequence unique to the NFAT5 transcript: 5′-CAACATGCCTGGAATTCAA-3′ (nt 335 to 353) [31]. As described, a control for non-specific siRNA effects, we used an siRNA targeting the green fluorescent protein (GFP), 5′- GGCTACGTCCAGGAGCGCACC-3′. MDM were transfected in 6-well plates using 1 µM of the indicated siRNA in siPORT NeoFX transfection reagent (Ambion Inc., www.ambion.com), prepared as recommended by the manufacturer, in a final volume of 750 µl in Macrophage-SFM medium plus 5% heat-inactivated human AB serum. The cultures were left at 37°C overnight after which cells were washed and incubated in fresh medium. MDM were transfected two times for efficient knock down of NFAT5 expression before infection experiments were performed [31]."}

sentences

{"project":"sentences","denotations":[{"id":"T4652","span":{"begin":687,"end":812},"obj":"Sentence"},{"id":"T4651","span":{"begin":585,"end":686},"obj":"Sentence"},{"id":"T4650","span":{"begin":306,"end":584},"obj":"Sentence"},{"id":"T4649","span":{"begin":156,"end":305},"obj":"Sentence"},{"id":"T4648","span":{"begin":108,"end":155},"obj":"Sentence"},{"id":"T4647","span":{"begin":0,"end":107},"obj":"Sentence"},{"id":"T65","span":{"begin":0,"end":107},"obj":"Sentence"},{"id":"T66","span":{"begin":108,"end":155},"obj":"Sentence"},{"id":"T67","span":{"begin":156,"end":305},"obj":"Sentence"},{"id":"T68","span":{"begin":306,"end":584},"obj":"Sentence"},{"id":"T69","span":{"begin":585,"end":686},"obj":"Sentence"},{"id":"T70","span":{"begin":687,"end":812},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"An siRNA was constructed (Ambion Inc., www.ambion.com) to target a sequence unique to the NFAT5 transcript: 5′-CAACATGCCTGGAATTCAA-3′ (nt 335 to 353) [31]. As described, a control for non-specific siRNA effects, we used an siRNA targeting the green fluorescent protein (GFP), 5′- GGCTACGTCCAGGAGCGCACC-3′. MDM were transfected in 6-well plates using 1 µM of the indicated siRNA in siPORT NeoFX transfection reagent (Ambion Inc., www.ambion.com), prepared as recommended by the manufacturer, in a final volume of 750 µl in Macrophage-SFM medium plus 5% heat-inactivated human AB serum. The cultures were left at 37°C overnight after which cells were washed and incubated in fresh medium. MDM were transfected two times for efficient knock down of NFAT5 expression before infection experiments were performed [31]."}

simple1

{"project":"simple1","denotations":[{"id":"T4669","span":{"begin":746,"end":751},"obj":"Protein"},{"id":"T4668","span":{"begin":270,"end":273},"obj":"Protein"},{"id":"T4667","span":{"begin":243,"end":268},"obj":"Protein"},{"id":"T4666","span":{"begin":90,"end":95},"obj":"Protein"}],"text":"An siRNA was constructed (Ambion Inc., www.ambion.com) to target a sequence unique to the NFAT5 transcript: 5′-CAACATGCCTGGAATTCAA-3′ (nt 335 to 353) [31]. As described, a control for non-specific siRNA effects, we used an siRNA targeting the green fluorescent protein (GFP), 5′- GGCTACGTCCAGGAGCGCACC-3′. MDM were transfected in 6-well plates using 1 µM of the indicated siRNA in siPORT NeoFX transfection reagent (Ambion Inc., www.ambion.com), prepared as recommended by the manufacturer, in a final volume of 750 µl in Macrophage-SFM medium plus 5% heat-inactivated human AB serum. The cultures were left at 37°C overnight after which cells were washed and incubated in fresh medium. MDM were transfected two times for efficient knock down of NFAT5 expression before infection experiments were performed [31]."}

BioNLP16_DUT

{"project":"BioNLP16_DUT","denotations":[{"id":"T5021","span":{"begin":752,"end":762},"obj":"Gene_expression"},{"id":"T5020","span":{"begin":229,"end":238},"obj":"Regulation"},{"id":"T5019","span":{"begin":746,"end":751},"obj":"Protein"},{"id":"T5018","span":{"begin":270,"end":273},"obj":"Protein"},{"id":"T5017","span":{"begin":243,"end":268},"obj":"Protein"},{"id":"T5016","span":{"begin":90,"end":95},"obj":"Protein"}],"relations":[{"id":"R3968","pred":"themeOf","subj":"T5017","obj":"T5020"},{"id":"R3969","pred":"themeOf","subj":"T5018","obj":"T5020"},{"id":"R3970","pred":"themeOf","subj":"T5019","obj":"T5021"}],"text":"An siRNA was constructed (Ambion Inc., www.ambion.com) to target a sequence unique to the NFAT5 transcript: 5′-CAACATGCCTGGAATTCAA-3′ (nt 335 to 353) [31]. As described, a control for non-specific siRNA effects, we used an siRNA targeting the green fluorescent protein (GFP), 5′- GGCTACGTCCAGGAGCGCACC-3′. MDM were transfected in 6-well plates using 1 µM of the indicated siRNA in siPORT NeoFX transfection reagent (Ambion Inc., www.ambion.com), prepared as recommended by the manufacturer, in a final volume of 750 µl in Macrophage-SFM medium plus 5% heat-inactivated human AB serum. The cultures were left at 37°C overnight after which cells were washed and incubated in fresh medium. MDM were transfected two times for efficient knock down of NFAT5 expression before infection experiments were performed [31]."}

BioNLP16_Messiy

{"project":"BioNLP16_Messiy","denotations":[{"id":"T4835","span":{"begin":229,"end":238},"obj":"Regulation"},{"id":"T4834","span":{"begin":223,"end":228},"obj":"Negative_regulation"},{"id":"T4833","span":{"begin":746,"end":751},"obj":"Protein"},{"id":"T4832","span":{"begin":270,"end":273},"obj":"Protein"},{"id":"T4831","span":{"begin":243,"end":268},"obj":"Protein"},{"id":"T4830","span":{"begin":90,"end":95},"obj":"Protein"},{"id":"T4837","span":{"begin":752,"end":762},"obj":"Gene_expression"},{"id":"T4836","span":{"begin":738,"end":742},"obj":"Negative_regulation"}],"relations":[{"id":"R3797","pred":"themeOf","subj":"T4831","obj":"T4835"},{"id":"R3798","pred":"themeOf","subj":"T4832","obj":"T4835"},{"id":"R3799","pred":"themeOf","subj":"T4833","obj":"T4837"},{"id":"R3800","pred":"themeOf","subj":"T4835","obj":"T4834"},{"id":"R3801","pred":"themeOf","subj":"T4835","obj":"T4834"},{"id":"R3802","pred":"themeOf","subj":"T4837","obj":"T4836"}],"text":"An siRNA was constructed (Ambion Inc., www.ambion.com) to target a sequence unique to the NFAT5 transcript: 5′-CAACATGCCTGGAATTCAA-3′ (nt 335 to 353) [31]. As described, a control for non-specific siRNA effects, we used an siRNA targeting the green fluorescent protein (GFP), 5′- GGCTACGTCCAGGAGCGCACC-3′. MDM were transfected in 6-well plates using 1 µM of the indicated siRNA in siPORT NeoFX transfection reagent (Ambion Inc., www.ambion.com), prepared as recommended by the manufacturer, in a final volume of 750 µl in Macrophage-SFM medium plus 5% heat-inactivated human AB serum. The cultures were left at 37°C overnight after which cells were washed and incubated in fresh medium. MDM were transfected two times for efficient knock down of NFAT5 expression before infection experiments were performed [31]."}

DLUT931

{"project":"DLUT931","denotations":[{"id":"T4829","span":{"begin":738,"end":742},"obj":"Negative_regulation"},{"id":"T4828","span":{"begin":752,"end":762},"obj":"Gene_expression"},{"id":"T4827","span":{"begin":229,"end":238},"obj":"Negative_regulation"},{"id":"T4826","span":{"begin":223,"end":228},"obj":"Negative_regulation"},{"id":"T4825","span":{"begin":746,"end":751},"obj":"Protein"},{"id":"T4824","span":{"begin":270,"end":273},"obj":"Protein"},{"id":"T4823","span":{"begin":243,"end":268},"obj":"Protein"},{"id":"T4822","span":{"begin":90,"end":95},"obj":"Protein"}],"relations":[{"id":"R3790","pred":"themeOf","subj":"T4823","obj":"T4826"},{"id":"R3791","pred":"themeOf","subj":"T4823","obj":"T4827"},{"id":"R3792","pred":"themeOf","subj":"T4824","obj":"T4827"},{"id":"R3793","pred":"themeOf","subj":"T4825","obj":"T4828"},{"id":"R3794","pred":"themeOf","subj":"T4827","obj":"T4826"},{"id":"R3795","pred":"themeOf","subj":"T4827","obj":"T4826"},{"id":"R3796","pred":"themeOf","subj":"T4828","obj":"T4829"}],"text":"An siRNA was constructed (Ambion Inc., www.ambion.com) to target a sequence unique to the NFAT5 transcript: 5′-CAACATGCCTGGAATTCAA-3′ (nt 335 to 353) [31]. As described, a control for non-specific siRNA effects, we used an siRNA targeting the green fluorescent protein (GFP), 5′- GGCTACGTCCAGGAGCGCACC-3′. MDM were transfected in 6-well plates using 1 µM of the indicated siRNA in siPORT NeoFX transfection reagent (Ambion Inc., www.ambion.com), prepared as recommended by the manufacturer, in a final volume of 750 µl in Macrophage-SFM medium plus 5% heat-inactivated human AB serum. The cultures were left at 37°C overnight after which cells were washed and incubated in fresh medium. MDM were transfected two times for efficient knock down of NFAT5 expression before infection experiments were performed [31]."}

bionlp-st-ge-2016-test-ihmc

{"project":"bionlp-st-ge-2016-test-ihmc","denotations":[{"id":"T5034","span":{"begin":746,"end":762},"obj":"Gene_expression"},{"id":"T5033","span":{"begin":638,"end":643},"obj":"Entity"},{"id":"T5032","span":{"begin":687,"end":690},"obj":"Protein"},{"id":"T5031","span":{"begin":381,"end":414},"obj":"Entity"},{"id":"T5030","span":{"begin":184,"end":210},"obj":"Protein"},{"id":"T5029","span":{"begin":746,"end":751},"obj":"Protein"},{"id":"T5028","span":{"begin":5,"end":8},"obj":"Protein"},{"id":"T5027","span":{"begin":270,"end":273},"obj":"Protein"},{"id":"T5026","span":{"begin":306,"end":309},"obj":"Protein"},{"id":"T5024","span":{"begin":243,"end":275},"obj":"Protein"},{"id":"T5023","span":{"begin":86,"end":106},"obj":"Protein"},{"id":"T5022","span":{"begin":90,"end":95},"obj":"Protein"}],"relations":[{"id":"R3971","pred":"themeOf","subj":"T5029","obj":"T5034"}],"text":"An siRNA was constructed (Ambion Inc., www.ambion.com) to target a sequence unique to the NFAT5 transcript: 5′-CAACATGCCTGGAATTCAA-3′ (nt 335 to 353) [31]. As described, a control for non-specific siRNA effects, we used an siRNA targeting the green fluorescent protein (GFP), 5′- GGCTACGTCCAGGAGCGCACC-3′. MDM were transfected in 6-well plates using 1 µM of the indicated siRNA in siPORT NeoFX transfection reagent (Ambion Inc., www.ambion.com), prepared as recommended by the manufacturer, in a final volume of 750 µl in Macrophage-SFM medium plus 5% heat-inactivated human AB serum. The cultures were left at 37°C overnight after which cells were washed and incubated in fresh medium. MDM were transfected two times for efficient knock down of NFAT5 expression before infection experiments were performed [31]."}

bionlp-st-ge-2016-spacy-parsed

bionlp-st-ge-2016-test-tees

{"project":"bionlp-st-ge-2016-test-tees","denotations":[{"id":"T4849","span":{"begin":738,"end":742},"obj":"Negative_regulation"},{"id":"T4848","span":{"begin":752,"end":762},"obj":"Gene_expression"},{"id":"T4847","span":{"begin":746,"end":751},"obj":"Protein"},{"id":"T4846","span":{"begin":381,"end":393},"obj":"Protein"},{"id":"T4845","span":{"begin":270,"end":273},"obj":"Protein"},{"id":"T4844","span":{"begin":243,"end":268},"obj":"Protein"},{"id":"T4843","span":{"begin":90,"end":106},"obj":"Protein"}],"relations":[{"id":"R3805","pred":"themeOf","subj":"T4847","obj":"T4848"},{"id":"R3806","pred":"themeOf","subj":"T4848","obj":"T4849"}],"text":"An siRNA was constructed (Ambion Inc., www.ambion.com) to target a sequence unique to the NFAT5 transcript: 5′-CAACATGCCTGGAATTCAA-3′ (nt 335 to 353) [31]. As described, a control for non-specific siRNA effects, we used an siRNA targeting the green fluorescent protein (GFP), 5′- GGCTACGTCCAGGAGCGCACC-3′. MDM were transfected in 6-well plates using 1 µM of the indicated siRNA in siPORT NeoFX transfection reagent (Ambion Inc., www.ambion.com), prepared as recommended by the manufacturer, in a final volume of 750 µl in Macrophage-SFM medium plus 5% heat-inactivated human AB serum. The cultures were left at 37°C overnight after which cells were washed and incubated in fresh medium. MDM were transfected two times for efficient knock down of NFAT5 expression before infection experiments were performed [31]."}

testone

{"project":"testone","denotations":[{"id":"T4636","span":{"begin":738,"end":742},"obj":"Negative_regulation"},{"id":"T4635","span":{"begin":746,"end":751},"obj":"Protein"},{"id":"T4634","span":{"begin":270,"end":273},"obj":"Protein"},{"id":"T4633","span":{"begin":243,"end":268},"obj":"Protein"},{"id":"T4632","span":{"begin":90,"end":95},"obj":"Protein"}],"relations":[{"id":"R3638","pred":"equivalentTo","subj":"T4634","obj":"T4633"}],"text":"An siRNA was constructed (Ambion Inc., www.ambion.com) to target a sequence unique to the NFAT5 transcript: 5′-CAACATGCCTGGAATTCAA-3′ (nt 335 to 353) [31]. As described, a control for non-specific siRNA effects, we used an siRNA targeting the green fluorescent protein (GFP), 5′- GGCTACGTCCAGGAGCGCACC-3′. MDM were transfected in 6-well plates using 1 µM of the indicated siRNA in siPORT NeoFX transfection reagent (Ambion Inc., www.ambion.com), prepared as recommended by the manufacturer, in a final volume of 750 µl in Macrophage-SFM medium plus 5% heat-inactivated human AB serum. The cultures were left at 37°C overnight after which cells were washed and incubated in fresh medium. MDM were transfected two times for efficient knock down of NFAT5 expression before infection experiments were performed [31]."}

test3

{"project":"test3","denotations":[{"id":"T4644","span":{"begin":746,"end":751},"obj":"Protein"},{"id":"T4643","span":{"begin":270,"end":273},"obj":"Protein"},{"id":"T4642","span":{"begin":243,"end":268},"obj":"Protein"},{"id":"T4641","span":{"begin":90,"end":95},"obj":"Protein"},{"id":"T4640","span":{"begin":746,"end":751},"obj":"Protein"},{"id":"T4639","span":{"begin":270,"end":273},"obj":"Protein"},{"id":"T4638","span":{"begin":243,"end":268},"obj":"Protein"},{"id":"T4637","span":{"begin":90,"end":95},"obj":"Protein"}],"relations":[{"id":"R3639","pred":"equivalentTo","subj":"T4643","obj":"T4642"}],"text":"An siRNA was constructed (Ambion Inc., www.ambion.com) to target a sequence unique to the NFAT5 transcript: 5′-CAACATGCCTGGAATTCAA-3′ (nt 335 to 353) [31]. As described, a control for non-specific siRNA effects, we used an siRNA targeting the green fluorescent protein (GFP), 5′- GGCTACGTCCAGGAGCGCACC-3′. MDM were transfected in 6-well plates using 1 µM of the indicated siRNA in siPORT NeoFX transfection reagent (Ambion Inc., www.ambion.com), prepared as recommended by the manufacturer, in a final volume of 750 µl in Macrophage-SFM medium plus 5% heat-inactivated human AB serum. The cultures were left at 37°C overnight after which cells were washed and incubated in fresh medium. MDM were transfected two times for efficient knock down of NFAT5 expression before infection experiments were performed [31]."}

PMC:3320587 / 10380-11192 JSONTXT

Annnotations TAB JSON ListView MergeView

PMC:3320587 / 10380-11192 JSON TXT