> top > docs > PMC:3320587 > spans > 10354-11192 > annotations

PMC:3320587 / 10354-11192 JSONTXT

Annnotations TAB JSON ListView MergeView

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T4814 836-837 -RRB- denotes ]
T4813 834-836 CD denotes 31
T4812 833-834 -LRB- denotes [
T4811 823-832 VB denotes performed
T4810 818-822 VB denotes were
T4809 806-817 NN denotes experiments
T4808 796-805 NN denotes infection
T4807 789-795 IN denotes before
T4806 778-788 NN denotes expression
T4805 772-777 NN denotes NFAT5
T4804 769-771 IN denotes of
T4803 764-768 RP denotes down
T4802 758-763 NN denotes knock
T4801 748-757 JJ denotes efficient
T4800 744-747 IN denotes for
T4799 738-743 NN denotes times
T4798 734-737 CD denotes two
T4797 722-733 VB denotes transfected
T4796 717-721 VB denotes were
T4795 713-716 NN denotes MDM
T4794 705-711 NN denotes medium
T4793 699-704 JJ denotes fresh
T4792 696-698 IN denotes in
T4791 686-695 VB denotes incubated
T4790 682-685 CC denotes and
T4789 675-681 VB denotes washed
T4788 670-674 VB denotes were
T4787 664-669 NN denotes cells
T4786 658-663 WDT denotes which
T4785 652-657 IN denotes after
T4784 642-651 RB denotes overnight
T4783 637-641 NN denotes 37°C
T4782 634-636 IN denotes at
T4781 629-633 VB denotes left
T4780 624-628 VB denotes were
T4779 615-623 NN denotes cultures
T4778 611-614 DT denotes The
T4777 604-609 NN denotes serum
T4776 601-603 NN denotes AB
T4775 595-600 JJ denotes human
T4774 578-594 JJ denotes heat-inactivated
T4773 576-577 NN denotes %
T4772 575-576 CD denotes 5
T4771 570-574 CC denotes plus
T4770 563-569 NN denotes medium
T4769 548-562 JJ denotes Macrophage-SFM
T4768 545-547 IN denotes in
T4767 542-544 NN denotes µl
T4766 538-541 CD denotes 750
T4765 535-537 IN denotes of
T4764 528-534 NN denotes volume
T4763 522-527 JJ denotes final
T4762 520-521 DT denotes a
T4761 517-519 IN denotes in
T4760 515-516 -COMMA- denotes ,
T4759 503-515 NN denotes manufacturer
T4758 499-502 DT denotes the
T4757 496-498 IN denotes by
T4756 484-495 VB denotes recommended
T4755 481-483 IN denotes as
T4754 472-480 VB denotes prepared
T4753 470-471 -COMMA- denotes ,
T4752 469-470 -RRB- denotes )
T4751 455-469 NN denotes www.ambion.com
T4750 453-454 -COMMA- denotes ,
T4749 449-453 NNP denotes Inc.
T4748 442-448 NNP denotes Ambion
T4747 441-442 -LRB- denotes (
T4746 433-440 NN denotes reagent
T4745 420-432 NN denotes transfection
T4744 414-419 NN denotes NeoFX
T4743 407-413 NN denotes siPORT
T4742 404-406 IN denotes in
T4741 398-403 NN denotes siRNA
T4740 388-397 VB denotes indicated
T4739 384-387 DT denotes the
T4738 381-383 IN denotes of
T4737 378-380 NN denotes µM
T4736 376-377 CD denotes 1
T4735 370-375 VB denotes using
T4734 363-369 NN denotes plates
T4733 356-362 JJ denotes 6-well
T4732 353-355 IN denotes in
T4731 341-352 VB denotes transfected
T4730 336-340 VB denotes were
T4729 332-335 NN denotes MDM
T4728 306-330 NN denotes GGCTACGTCCAGGAGCGCACC-3′
T4727 302-305 JJ denotes 5′-
T4726 300-301 -COMMA- denotes ,
T4725 299-300 -RRB- denotes )
T4724 296-299 NN denotes GFP
T4723 295-296 -LRB- denotes (
T4722 287-294 NN denotes protein
T4721 275-286 JJ denotes fluorescent
T4720 269-274 JJ denotes green
T4719 265-268 DT denotes the
T4718 255-264 VB denotes targeting
T4717 249-254 NN denotes siRNA
T4716 246-248 DT denotes an
T4715 241-245 VB denotes used
T4714 238-240 PRP denotes we
T4713 236-237 -COMMA- denotes ,
T4712 229-236 NN denotes effects
T4711 223-228 NN denotes siRNA
T4710 210-222 JJ denotes non-specific
T4709 206-209 IN denotes for
T4708 198-205 NN denotes control
T4707 196-197 DT denotes a
T4706 194-195 -COMMA- denotes ,
T4705 185-194 VB denotes described
T4704 182-184 IN denotes As
T4703 179-180 -RRB- denotes ]
T4702 177-179 CD denotes 31
T4701 176-177 -LRB- denotes [
T4700 174-175 -RRB- denotes )
T4699 171-174 CD denotes 353
T4698 168-170 TO denotes to
T4697 164-167 CD denotes 335
T4696 161-163 NN denotes nt
T4695 160-161 -LRB- denotes (
T4694 134-159 NN denotes 5′-CAACATGCCTGGAATTCAA-3′
T4693 132-133 -COLON- denotes :
T4692 122-132 NN denotes transcript
T4691 116-121 NN denotes NFAT5
T4690 112-115 DT denotes the
T4689 109-111 TO denotes to
T4688 102-108 JJ denotes unique
T4687 93-101 NN denotes sequence
T4686 91-92 DT denotes a
T4685 84-90 VB denotes target
T4684 81-83 TO denotes to
T4683 79-80 -RRB- denotes )
T4682 65-79 NN denotes www.ambion.com
T4681 63-64 -COMMA- denotes ,
T4680 59-63 NNP denotes Inc.
T4679 52-58 NNP denotes Ambion
T4678 51-52 -LRB- denotes (
T4677 39-50 VB denotes constructed
T4676 35-38 VB denotes was
T4675 29-34 NN denotes siRNA
T4674 26-28 DT denotes An
T4673 22-25 NN denotes MDM
T4672 19-21 IN denotes of
T4671 6-18 NN denotes transfection
T4670 0-5 NN denotes siRNA
R3645 T4671 T4670 arg1Of transfection,siRNA
R3646 T4671 T4672 arg1Of transfection,of
R3647 T4673 T4672 arg2Of MDM,of
R3648 T4675 T4674 arg1Of siRNA,An
R3649 T4675 T4676 arg1Of siRNA,was
R3650 T4675 T4677 arg2Of siRNA,constructed
R3651 T4677 T4676 arg2Of constructed,was
R3652 T4677 T4678 arg1Of constructed,(
R3653 T4677 T4684 modOf constructed,to
R3654 T4677 T4693 arg1Of constructed,:
R3655 T4680 T4678 arg2Of Inc.,(
R3656 T4680 T4679 arg1Of Inc.,Ambion
R3657 T4680 T4681 arg1Of Inc.,","
R3658 T4682 T4681 arg2Of www.ambion.com,","
R3659 T4683 T4678 arg3Of ),(
R3660 T4685 T4684 arg1Of target,to
R3661 T4687 T4685 arg2Of sequence,target
R3662 T4687 T4686 arg1Of sequence,a
R3663 T4687 T4688 arg1Of sequence,unique
R3664 T4688 T4689 arg1Of unique,to
R3665 T4692 T4689 arg2Of transcript,to
R3666 T4692 T4690 arg1Of transcript,the
R3667 T4692 T4691 arg1Of transcript,NFAT5
R3668 T4694 T4695 arg1Of 5′-CAACATGCCTGGAATTCAA-3′,(
R3669 T4694 T4701 arg1Of 5′-CAACATGCCTGGAATTCAA-3′,[
R3670 T4699 T4695 arg2Of 353,(
R3671 T4699 T4696 arg1Of 353,nt
R3672 T4699 T4697 arg1Of 353,335
R3673 T4699 T4698 arg1Of 353,to
R3674 T4700 T4695 arg3Of ),(
R3675 T4702 T4701 arg2Of 31,[
R3676 T4703 T4701 arg3Of ],[
R3677 T4705 T4704 arg2Of described,As
R3678 T4705 T4706 arg1Of described,","
R3679 T4708 T4706 arg2Of control,","
R3680 T4708 T4707 arg1Of control,a
R3681 T4708 T4709 arg1Of control,for
R3682 T4712 T4709 arg2Of effects,for
R3683 T4712 T4710 arg1Of effects,non-specific
R3684 T4712 T4711 arg1Of effects,siRNA
R3685 T4714 T4715 arg1Of we,used
R3686 T4715 T4704 arg1Of used,As
R3687 T4715 T4713 arg1Of used,","
R3688 T4717 T4715 arg2Of siRNA,used
R3689 T4717 T4716 arg1Of siRNA,an
R3690 T4717 T4718 arg1Of siRNA,targeting
R3691 T4722 T4718 arg2Of protein,targeting
R3692 T4722 T4719 arg1Of protein,the
R3693 T4722 T4720 arg1Of protein,green
R3694 T4722 T4721 arg1Of protein,fluorescent
R3695 T4722 T4723 arg1Of protein,(
R3696 T4722 T4726 arg1Of protein,","
R3697 T4724 T4723 arg2Of GFP,(
R3698 T4725 T4723 arg3Of ),(
R3699 T4728 T4726 arg2Of GGCTACGTCCAGGAGCGCACC-3′,","
R3700 T4728 T4727 arg1Of GGCTACGTCCAGGAGCGCACC-3′,5′-
R3701 T4729 T4730 arg1Of MDM,were
R3702 T4729 T4731 arg2Of MDM,transfected
R3703 T4731 T4730 arg2Of transfected,were
R3704 T4731 T4732 arg1Of transfected,in
R3705 T4731 T4760 arg1Of transfected,","
R3706 T4731 T4761 arg1Of transfected,in
R3707 T4734 T4732 arg2Of plates,in
R3708 T4734 T4733 arg1Of plates,6-well
R3709 T4734 T4735 arg1Of plates,using
R3710 T4734 T4753 arg1Of plates,","
R3711 T4734 T4754 arg2Of plates,prepared
R3712 T4737 T4735 arg2Of µM,using
R3713 T4737 T4736 arg1Of µM,1
R3714 T4737 T4738 arg1Of µM,of
R3715 T4737 T4742 arg1Of µM,in
R3716 T4741 T4738 arg2Of siRNA,of
R3717 T4741 T4739 arg1Of siRNA,the
R3718 T4741 T4740 arg2Of siRNA,indicated
R3719 T4746 T4742 arg2Of reagent,in
R3720 T4746 T4743 arg1Of reagent,siPORT
R3721 T4746 T4744 arg1Of reagent,NeoFX
R3722 T4746 T4745 arg1Of reagent,transfection
R3723 T4746 T4747 arg1Of reagent,(
R3724 T4749 T4747 arg2Of Inc.,(
R3725 T4749 T4748 arg1Of Inc.,Ambion
R3726 T4749 T4750 arg1Of Inc.,","
R3727 T4751 T4750 arg2Of www.ambion.com,","
R3728 T4752 T4747 arg3Of ),(
R3729 T4754 T4755 arg1Of prepared,as
R3730 T4756 T4755 arg2Of recommended,as
R3731 T4759 T4756 arg1Of manufacturer,recommended
R3732 T4759 T4757 arg2Of manufacturer,by
R3733 T4759 T4758 arg1Of manufacturer,the
R3734 T4764 T4761 arg2Of volume,in
R3735 T4764 T4762 arg1Of volume,a
R3736 T4764 T4763 arg1Of volume,final
R3737 T4764 T4765 arg1Of volume,of
R3738 T4764 T4768 arg1Of volume,in
R3739 T4767 T4765 arg2Of µl,of
R3740 T4767 T4766 arg1Of µl,750
R3741 T4770 T4769 arg1Of medium,Macrophage-SFM
R3742 T4770 T4771 arg1Of medium,plus
R3743 T4771 T4768 arg2Of plus,in
R3744 T4772 T4773 arg1Of 5,%
R3745 T4777 T4771 arg2Of serum,plus
R3746 T4777 T4772 arg1Of serum,5
R3747 T4777 T4774 arg1Of serum,heat-inactivated
R3748 T4777 T4775 arg1Of serum,human
R3749 T4777 T4776 arg1Of serum,AB
R3750 T4779 T4778 arg1Of cultures,The
R3751 T4779 T4780 arg1Of cultures,were
R3752 T4779 T4781 arg2Of cultures,left
R3753 T4781 T4780 arg2Of left,were
R3754 T4781 T4782 arg1Of left,at
R3755 T4781 T4784 arg1Of left,overnight
R3756 T4783 T4782 arg2Of 37°C,at
R3757 T4786 T4781 arg3Of which,left
R3758 T4786 T4785 arg2Of which,after
R3759 T4787 T4788 arg1Of cells,were
R3760 T4787 T4789 arg2Of cells,washed
R3761 T4787 T4791 arg2Of cells,incubated
R3762 T4789 T4790 arg1Of washed,and
R3763 T4790 T4785 arg1Of and,after
R3764 T4790 T4788 arg2Of and,were
R3765 T4790 T4792 arg1Of and,in
R3766 T4791 T4790 arg2Of incubated,and
R3767 T4794 T4792 arg2Of medium,in
R3768 T4794 T4793 arg1Of medium,fresh
R3769 T4795 T4796 arg1Of MDM,were
R3770 T4795 T4797 arg2Of MDM,transfected
R3771 T4797 T4796 arg2Of transfected,were
R3772 T4797 T4799 arg1Of transfected,times
R3773 T4797 T4800 arg1Of transfected,for
R3774 T4797 T4803 arg1Of transfected,down
R3775 T4797 T4807 arg1Of transfected,before
R3776 T4799 T4798 arg1Of times,two
R3777 T4802 T4800 arg2Of knock,for
R3778 T4802 T4801 arg1Of knock,efficient
R3779 T4803 T4804 arg1Of down,of
R3780 T4806 T4804 arg2Of expression,of
R3781 T4806 T4805 arg1Of expression,NFAT5
R3782 T4809 T4808 arg1Of experiments,infection
R3783 T4809 T4810 arg1Of experiments,were
R3784 T4809 T4811 arg2Of experiments,performed
R3785 T4811 T4807 arg2Of performed,before
R3786 T4811 T4810 arg2Of performed,were
R3787 T4811 T4812 arg1Of performed,[
R3788 T4813 T4812 arg2Of 31,[
R3789 T4814 T4812 arg3Of ],[

bionlp-st-ge-2016-test-proteins

Id Subject Object Predicate Lexical cue
T4664 772-777 Protein denotes NFAT5
T4663 296-299 Protein denotes GFP
T4662 269-294 Protein denotes green fluorescent protein
T4661 116-121 Protein denotes NFAT5

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T4817 296-299 http://www.uniprot.org/uniprot/Q9U6Y4 denotes GFP
T4816 772-777 http://www.uniprot.org/uniprot/O94916 denotes NFAT5
T4815 116-121 http://www.uniprot.org/uniprot/O94916 denotes NFAT5

UBERON-AE

Id Subject Object Predicate Lexical cue
T4645 604-609 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

GO-CC

Id Subject Object Predicate Lexical cue
T4665 664-669 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

sentences

Id Subject Object Predicate Lexical cue
T4652 713-838 Sentence denotes MDM were transfected two times for efficient knock down of NFAT5 expression before infection experiments were performed [31].
T4651 611-712 Sentence denotes The cultures were left at 37°C overnight after which cells were washed and incubated in fresh medium.
T4650 332-610 Sentence denotes MDM were transfected in 6-well plates using 1 µM of the indicated siRNA in siPORT NeoFX transfection reagent (Ambion Inc., www.ambion.com), prepared as recommended by the manufacturer, in a final volume of 750 µl in Macrophage-SFM medium plus 5% heat-inactivated human AB serum.
T4649 182-331 Sentence denotes As described, a control for non-specific siRNA effects, we used an siRNA targeting the green fluorescent protein (GFP), 5′- GGCTACGTCCAGGAGCGCACC-3′.
T4648 134-181 Sentence denotes 5′-CAACATGCCTGGAATTCAA-3′ (nt 335 to 353) [31].
T4647 26-133 Sentence denotes An siRNA was constructed (Ambion Inc., www.ambion.com) to target a sequence unique to the NFAT5 transcript:
T4646 0-25 Sentence denotes siRNA transfection of MDM
T64 0-25 Sentence denotes siRNA transfection of MDM
T65 26-133 Sentence denotes An siRNA was constructed (Ambion Inc., www.ambion.com) to target a sequence unique to the NFAT5 transcript:
T66 134-181 Sentence denotes 5′-CAACATGCCTGGAATTCAA-3′ (nt 335 to 353) [31].
T67 182-331 Sentence denotes As described, a control for non-specific siRNA effects, we used an siRNA targeting the green fluorescent protein (GFP), 5′- GGCTACGTCCAGGAGCGCACC-3′.
T68 332-610 Sentence denotes MDM were transfected in 6-well plates using 1 µM of the indicated siRNA in siPORT NeoFX transfection reagent (Ambion Inc., www.ambion.com), prepared as recommended by the manufacturer, in a final volume of 750 µl in Macrophage-SFM medium plus 5% heat-inactivated human AB serum.
T69 611-712 Sentence denotes The cultures were left at 37°C overnight after which cells were washed and incubated in fresh medium.
T70 713-838 Sentence denotes MDM were transfected two times for efficient knock down of NFAT5 expression before infection experiments were performed [31].

simple1

Id Subject Object Predicate Lexical cue
T4669 772-777 Protein denotes NFAT5
T4668 296-299 Protein denotes GFP
T4667 269-294 Protein denotes green fluorescent protein
T4666 116-121 Protein denotes NFAT5

BioNLP16_DUT

Id Subject Object Predicate Lexical cue
T5021 778-788 Gene_expression denotes expression
T5020 255-264 Regulation denotes targeting
T5019 772-777 Protein denotes NFAT5
T5018 296-299 Protein denotes GFP
T5017 269-294 Protein denotes green fluorescent protein
T5016 116-121 Protein denotes NFAT5
R3968 T5017 T5020 themeOf green fluorescent protein,targeting
R3969 T5018 T5020 themeOf GFP,targeting
R3970 T5019 T5021 themeOf NFAT5,expression

BioNLP16_Messiy

Id Subject Object Predicate Lexical cue
T4835 255-264 Regulation denotes targeting
T4834 249-254 Negative_regulation denotes siRNA
T4833 772-777 Protein denotes NFAT5
T4832 296-299 Protein denotes GFP
T4831 269-294 Protein denotes green fluorescent protein
T4830 116-121 Protein denotes NFAT5
T4837 778-788 Gene_expression denotes expression
T4836 764-768 Negative_regulation denotes down
R3797 T4831 T4835 themeOf green fluorescent protein,targeting
R3798 T4832 T4835 themeOf GFP,targeting
R3799 T4833 T4837 themeOf NFAT5,expression
R3800 T4835 T4834 themeOf targeting,siRNA
R3801 T4835 T4834 themeOf targeting,siRNA
R3802 T4837 T4836 themeOf expression,down

DLUT931

Id Subject Object Predicate Lexical cue
T4829 764-768 Negative_regulation denotes down
T4828 778-788 Gene_expression denotes expression
T4827 255-264 Negative_regulation denotes targeting
T4826 249-254 Negative_regulation denotes siRNA
T4825 772-777 Protein denotes NFAT5
T4824 296-299 Protein denotes GFP
T4823 269-294 Protein denotes green fluorescent protein
T4822 116-121 Protein denotes NFAT5
R3790 T4823 T4826 themeOf green fluorescent protein,siRNA
R3791 T4823 T4827 themeOf green fluorescent protein,targeting
R3792 T4824 T4827 themeOf GFP,targeting
R3793 T4825 T4828 themeOf NFAT5,expression
R3794 T4827 T4826 themeOf targeting,siRNA
R3795 T4827 T4826 themeOf targeting,siRNA
R3796 T4828 T4829 themeOf expression,down

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T5034 772-788 Gene_expression denotes NFAT5 expression
T5033 664-669 Entity denotes cells
T5032 713-716 Protein denotes MDM
T5031 407-440 Entity denotes siPORT NeoFX transfection reagent
T5030 210-236 Protein denotes non-specific siRNA effects
T5029 772-777 Protein denotes NFAT5
T5028 31-34 Protein denotes RNA
T5027 296-299 Protein denotes GFP
T5026 332-335 Protein denotes MDM
T5025 19-25 Protein denotes of MDM
T5024 269-301 Protein denotes green fluorescent protein (GFP),
T5023 112-132 Protein denotes the NFAT5 transcript
T5022 116-121 Protein denotes NFAT5
R3971 T5029 T5034 themeOf NFAT5,NFAT5 expression

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T5015 837-838 . denotes .
T5014 836-837 NNP denotes ]
T5013 834-836 CD denotes 31
T5012 833-834 NNP denotes [
T5011 823-832 VBN denotes performed
T5010 818-822 VBD denotes were
T5009 806-817 NNS denotes experiments
T5008 796-805 NN denotes infection
T5007 789-795 IN denotes before
T5006 778-788 NN denotes expression
T5005 772-777 CD denotes NFAT5
T5004 769-771 IN denotes of
T5003 764-768 IN denotes down
T5002 758-763 VB denotes knock
T5001 748-757 JJ denotes efficient
T5000 744-747 IN denotes for
T4999 738-743 NNS denotes times
T4998 734-737 CD denotes two
T4997 722-733 VBN denotes transfected
T4996 717-721 VBD denotes were
T4995 713-716 NNP denotes MDM
T4994 711-712 . denotes .
T4993 705-711 NN denotes medium
T4992 699-704 JJ denotes fresh
T4991 696-698 IN denotes in
T4990 686-695 VBN denotes incubated
T4989 682-685 CC denotes and
T4988 675-681 VBN denotes washed
T4987 670-674 VBD denotes were
T4986 664-669 NNS denotes cells
T4985 658-663 WDT denotes which
T4984 652-657 IN denotes after
T4983 642-651 RB denotes overnight
T4982 640-641 NNP denotes C
T4981 639-640 CD denotes °
T4980 637-639 CD denotes 37
T4979 634-636 IN denotes at
T4978 629-633 VBN denotes left
T4977 624-628 VBD denotes were
T4976 615-623 NNS denotes cultures
T4975 611-614 DT denotes The
T4974 609-610 . denotes .
T4973 604-609 NN denotes serum
T4972 601-603 NNP denotes AB
T4971 595-600 JJ denotes human
T4970 578-594 JJ denotes heat-inactivated
T4969 576-577 NN denotes %
T4968 575-576 CD denotes 5
T4967 570-574 CC denotes plus
T4966 563-569 NN denotes medium
T4965 548-562 NNP denotes Macrophage-SFM
T4964 545-547 IN denotes in
T4963 542-544 NN denotes µl
T4962 538-541 CD denotes 750
T4961 535-537 IN denotes of
T4960 528-534 NN denotes volume
T4959 522-527 JJ denotes final
T4958 520-521 DT denotes a
T4957 517-519 IN denotes in
T4956 515-516 , denotes ,
T4955 503-515 NN denotes manufacturer
T4954 499-502 DT denotes the
T4953 496-498 IN denotes by
T4952 484-495 VBN denotes recommended
T4951 481-483 IN denotes as
T4950 472-480 VBN denotes prepared
T4949 470-471 , denotes ,
T4948 469-470 -RRB- denotes )
T4947 455-469 NN denotes www.ambion.com
T4946 453-454 , denotes ,
T4945 449-453 NNP denotes Inc.
T4944 442-448 NNP denotes Ambion
T4943 441-442 -LRB- denotes (
T4888 164-167 CD denotes 335
T4887 161-163 JJ denotes nt
T4886 160-161 -LRB- denotes (
T4885 158-159 NN denotes ′
T4884 137-158 NN denotes CAACATGCCTGGAATTCAA-3
T4883 136-137 : denotes -
T4882 135-136 SYM denotes ′
T4881 134-135 CD denotes 5
T4880 132-133 : denotes :
T4879 122-132 NN denotes transcript
T4878 116-121 NNP denotes NFAT5
T4877 112-115 DT denotes the
T4876 109-111 TO denotes to
T4875 102-108 JJ denotes unique
T4874 93-101 NN denotes sequence
T4873 91-92 DT denotes a
T4872 84-90 VB denotes target
T4871 81-83 TO denotes to
T4870 79-80 -RRB- denotes )
T4869 65-79 NN denotes www.ambion.com
T4868 63-64 , denotes ,
T4867 59-63 NNP denotes Inc.
T4866 52-58 NNP denotes Ambion
T4865 51-52 -LRB- denotes (
T4864 39-50 VBN denotes constructed
T4863 35-38 VBD denotes was
T4862 29-34 NN denotes siRNA
T4861 26-28 DT denotes An
T4860 22-25 NNP denotes MDM
T4859 19-21 IN denotes of
T4858 6-18 NN denotes transfection
T4857 0-5 NNP denotes siRNA
T4942 433-440 NN denotes reagent
T4941 420-432 NN denotes transfection
T4940 414-419 NNP denotes NeoFX
T4939 407-413 NNP denotes siPORT
T4938 404-406 IN denotes in
T4937 398-403 NN denotes siRNA
T4936 388-397 VBN denotes indicated
T4935 384-387 DT denotes the
T4934 381-383 IN denotes of
T4933 378-380 NN denotes µM
T4932 376-377 CD denotes 1
T4931 370-375 VBG denotes using
T4930 363-369 NNS denotes plates
T4929 356-362 JJ denotes 6-well
T4928 353-355 IN denotes in
T4927 341-352 VBN denotes transfected
T4926 336-340 VBD denotes were
T4925 332-335 NNP denotes MDM
T4924 330-331 . denotes .
T4923 329-330 NN denotes ′
T4922 306-329 NN denotes GGCTACGTCCAGGAGCGCACC-3
T4921 304-305 : denotes -
T4920 303-304 SYM denotes ′
T4919 302-303 CD denotes 5
T4918 300-301 , denotes ,
T4917 299-300 -RRB- denotes )
T4916 296-299 NNP denotes GFP
T4915 295-296 -LRB- denotes (
T4914 287-294 NN denotes protein
T4913 275-286 JJ denotes fluorescent
T4912 269-274 JJ denotes green
T4911 265-268 DT denotes the
T4910 255-264 VBG denotes targeting
T4909 249-254 JJ denotes siRNA
T4908 246-248 DT denotes an
T4907 241-245 VBD denotes used
T4906 238-240 PRP denotes we
T4905 236-237 , denotes ,
T4904 229-236 NNS denotes effects
T4903 223-228 NNP denotes siRNA
T4902 210-222 JJ denotes non-specific
T4901 206-209 IN denotes for
T4900 198-205 NN denotes control
T4899 196-197 DT denotes a
T4898 194-195 , denotes ,
T4897 185-194 VBN denotes described
T4896 182-184 IN denotes As
T4895 180-181 . denotes .
T4894 179-180 NNP denotes ]
T4893 177-179 CD denotes 31
T4892 176-177 NNP denotes [
T4891 174-175 -RRB- denotes )
T4890 171-174 CD denotes 353
T4889 168-170 TO denotes to
R3809 T4857 T4858 compound siRNA,transfection
R3810 T4858 T4864 nsubjpass transfection,constructed
R3811 T4859 T4858 prep of,transfection
R3812 T4860 T4859 pobj MDM,of
R3813 T4861 T4862 det An,siRNA
R3814 T4862 T4864 nsubjpass siRNA,constructed
R3815 T4863 T4864 auxpass was,constructed
R3816 T4864 T4864 ROOT constructed,constructed
R3817 T4865 T4867 punct (,Inc.
R3818 T4866 T4867 compound Ambion,Inc.
R3819 T4867 T4864 oprd Inc.,constructed
R3820 T4868 T4867 punct ",",Inc.
R3821 T4869 T4867 appos www.ambion.com,Inc.
R3822 T4870 T4867 punct ),Inc.
R3823 T4871 T4872 aux to,target
R3824 T4872 T4864 xcomp target,constructed
R3825 T4873 T4874 det a,sequence
R3826 T4874 T4872 dobj sequence,target
R3827 T4875 T4874 amod unique,sequence
R3828 T4876 T4875 prep to,unique
R3829 T4877 T4879 det the,transcript
R3830 T4878 T4879 compound NFAT5,transcript
R3831 T4879 T4876 pobj transcript,to
R3832 T4880 T4879 punct :,transcript
R3833 T4881 T4885 nummod 5,′
R3834 T4882 T4884 punct ′,CAACATGCCTGGAATTCAA-3
R3835 T4883 T4884 punct -,CAACATGCCTGGAATTCAA-3
R3836 T4884 T4885 compound CAACATGCCTGGAATTCAA-3,′
R3837 T4885 T4879 appos ′,transcript
R3838 T4886 T4887 punct (,nt
R3839 T4887 T4885 appos nt,′
R3840 T4888 T4890 quantmod 335,353
R3841 T4889 T4890 quantmod to,353
R3842 T4890 T4887 npadvmod 353,nt
R3843 T4891 T4879 punct ),transcript
R3844 T4892 T4876 pobj [,to
R3845 T4893 T4894 nummod 31,]
R3846 T4894 T4892 dobj ],[
R3847 T4895 T4864 punct .,constructed
R3848 T4896 T4897 mark As,described
R3849 T4897 T4907 advcl described,used
R3850 T4898 T4907 punct ",",used
R3851 T4899 T4900 det a,control
R3852 T4900 T4907 npadvmod control,used
R3853 T4901 T4900 prep for,control
R3854 T4902 T4904 amod non-specific,effects
R3855 T4903 T4904 compound siRNA,effects
R3856 T4904 T4901 pobj effects,for
R3857 T4905 T4907 punct ",",used
R3858 T4906 T4907 nsubj we,used
R3859 T4907 T4907 ROOT used,used
R3860 T4908 T4909 det an,siRNA
R3861 T4909 T4907 dobj siRNA,used
R3862 T4910 T4909 acl targeting,siRNA
R3863 T4911 T4914 det the,protein
R3864 T4912 T4914 amod green,protein
R3865 T4913 T4914 amod fluorescent,protein
R3866 T4914 T4910 dobj protein,targeting
R3867 T4915 T4914 punct (,protein
R3868 T4916 T4914 appos GFP,protein
R3869 T4917 T4914 punct ),protein
R3870 T4918 T4914 punct ",",protein
R3871 T4919 T4922 nummod 5,GGCTACGTCCAGGAGCGCACC-3
R3872 T4920 T4922 punct ′,GGCTACGTCCAGGAGCGCACC-3
R3873 T4921 T4922 punct -,GGCTACGTCCAGGAGCGCACC-3
R3874 T4922 T4923 compound GGCTACGTCCAGGAGCGCACC-3,′
R3875 T4923 T4914 appos ′,protein
R3876 T4924 T4909 punct .,siRNA
R3877 T4925 T4927 nsubjpass MDM,transfected
R3878 T4926 T4927 auxpass were,transfected
R3879 T4927 T4927 ROOT transfected,transfected
R3880 T4928 T4927 prep in,transfected
R3881 T4929 T4930 amod 6-well,plates
R3882 T4930 T4928 pobj plates,in
R3883 T4931 T4927 advcl using,transfected
R3884 T4932 T4933 nummod 1,µM
R3885 T4933 T4931 dobj µM,using
R3886 T4934 T4933 prep of,µM
R3887 T4935 T4937 det the,siRNA
R3888 T4936 T4937 amod indicated,siRNA
R3889 T4937 T4934 pobj siRNA,of
R3890 T4938 T4937 prep in,siRNA
R3891 T4939 T4942 nmod siPORT,reagent
R3892 T4940 T4942 nmod NeoFX,reagent
R3893 T4941 T4942 compound transfection,reagent
R3894 T4942 T4945 nmod reagent,Inc.
R3895 T4943 T4945 punct (,Inc.
R3896 T4944 T4945 compound Ambion,Inc.
R3897 T4945 T4938 pobj Inc.,in
R3898 T4946 T4945 punct ",",Inc.
R3899 T4947 T4945 appos www.ambion.com,Inc.
R3900 T4948 T4945 punct ),Inc.
R3901 T4949 T4933 punct ",",µM
R3902 T4950 T4927 conj prepared,transfected
R3903 T4951 T4952 mark as,recommended
R3904 T4952 T4950 advcl recommended,prepared
R3905 T4953 T4952 agent by,recommended
R3906 T4954 T4955 det the,manufacturer
R3907 T4955 T4953 pobj manufacturer,by
R3908 T4956 T4950 punct ",",prepared
R3909 T4957 T4950 prep in,prepared
R3910 T4958 T4960 det a,volume
R3911 T4959 T4960 amod final,volume
R3912 T4960 T4957 pobj volume,in
R3913 T4961 T4960 prep of,volume
R3914 T4962 T4963 nummod 750,µl
R3915 T4963 T4961 pobj µl,of
R3916 T4964 T4963 prep in,µl
R3917 T4965 T4966 compound Macrophage-SFM,medium
R3918 T4966 T4964 pobj medium,in
R3919 T4967 T4927 cc plus,transfected
R3920 T4968 T4969 nummod 5,%
R3921 T4969 T4973 nmod %,serum
R3922 T4970 T4973 amod heat-inactivated,serum
R3923 T4971 T4972 compound human,AB
R3924 T4972 T4973 compound AB,serum
R3925 T4973 T4927 conj serum,transfected
R3926 T4974 T4927 punct .,transfected
R3927 T4975 T4976 det The,cultures
R3928 T4976 T4978 nsubjpass cultures,left
R3929 T4977 T4978 auxpass were,left
R3930 T4978 T4978 ROOT left,left
R3931 T4979 T4978 prep at,left
R3932 T4980 T4981 nummod 37,°
R3933 T4981 T4982 nummod °,C
R3934 T4982 T4979 pobj C,at
R3935 T4983 T4978 advmod overnight,left
R3936 T4984 T4988 prep after,washed
R3937 T4985 T4984 pobj which,after
R3938 T4986 T4988 nsubjpass cells,washed
R3939 T4987 T4988 auxpass were,washed
R3940 T4988 T4978 advcl washed,left
R3941 T4989 T4988 cc and,washed
R3942 T4990 T4988 conj incubated,washed
R3943 T4991 T4990 prep in,incubated
R3944 T4992 T4993 amod fresh,medium
R3945 T4993 T4991 pobj medium,in
R3946 T4994 T4978 punct .,left
R3947 T4995 T4997 nsubjpass MDM,transfected
R3948 T4996 T4997 auxpass were,transfected
R3949 T4997 T4997 ROOT transfected,transfected
R3950 T4998 T4999 nummod two,times
R3951 T4999 T4997 npadvmod times,transfected
R3952 T5000 T4999 prep for,times
R3953 T5001 T5002 nsubj efficient,knock
R3954 T5002 T4997 conj knock,transfected
R3955 T5003 T5002 advmod down,knock
R3956 T5004 T5003 prep of,down
R3957 T5005 T5006 nummod NFAT5,expression
R3958 T5006 T5004 pobj expression,of
R3959 T5007 T5011 mark before,performed
R3960 T5008 T5009 compound infection,experiments
R3961 T5009 T5011 nsubjpass experiments,performed
R3962 T5010 T5011 auxpass were,performed
R3963 T5011 T5002 advcl performed,knock
R3964 T5012 T5014 nmod [,]
R3965 T5013 T5014 nummod 31,]
R3966 T5014 T5011 dobj ],performed
R3967 T5015 T4997 punct .,transfected

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T4849 764-768 Negative_regulation denotes down
T4848 778-788 Gene_expression denotes expression
T4847 772-777 Protein denotes NFAT5
T4846 407-419 Protein denotes siPORT NeoFX
T4845 296-299 Protein denotes GFP
T4844 269-294 Protein denotes green fluorescent protein
T4843 116-132 Protein denotes NFAT5 transcript
R3805 T4847 T4848 themeOf NFAT5,expression
R3806 T4848 T4849 themeOf expression,down

testone

Id Subject Object Predicate Lexical cue
T4636 764-768 Negative_regulation denotes down
T4635 772-777 Protein denotes NFAT5
T4634 296-299 Protein denotes GFP
T4633 269-294 Protein denotes green fluorescent protein
T4632 116-121 Protein denotes NFAT5
R3638 T4634 T4633 equivalentTo GFP,green fluorescent protein

test3

Id Subject Object Predicate Lexical cue
T4644 772-777 Protein denotes NFAT5
T4643 296-299 Protein denotes GFP
T4642 269-294 Protein denotes green fluorescent protein
T4641 116-121 Protein denotes NFAT5
T4640 772-777 Protein denotes NFAT5
T4639 296-299 Protein denotes GFP
T4638 269-294 Protein denotes green fluorescent protein
T4637 116-121 Protein denotes NFAT5
R3639 T4643 T4642 equivalentTo GFP,green fluorescent protein