PMC:3320587 / 0-19038 JSON TXT

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2_test

Id	Subject	Object	Predicate	Lexical cue
22496647-17359235-98010766	2900-2901	17359235	denotes	3
22496647-1906500-98010767	3150-3151	1906500	denotes	4
22496647-11854362-98010768	3154-3155	11854362	denotes	6
22496647-17263638-98010769	3326-3327	17263638	denotes	7
22496647-21852682-98010770	3347-3349	21852682	denotes	10
22496647-1345800-98010771	3546-3548	1345800	denotes	11
22496647-20299961-98010772	3551-3553	20299961	denotes	15
22496647-16622205-98010773	3667-3669	16622205	denotes	16
22496647-10426995-98010774	3672-3674	10426995	denotes	20
22496647-10588727-98010775	3858-3860	10588727	denotes	21
22496647-9430229-98010776	3863-3865	9430229	denotes	23
22496647-21852682-98010777	3881-3883	21852682	denotes	10
22496647-14751757-98010778	3887-3889	14751757	denotes	24
22496647-8837778-98010779	4183-4185	8837778	denotes	22
22496647-9734363-98010780	4189-4191	9734363	denotes	25
22496647-7982981-98010781	4194-4196	7982981	denotes	27
22496647-21852682-98010782	4212-4214	21852682	denotes	10
22496647-21297424-98010783	4754-4756	21297424	denotes	28
22496647-12359721-98010784	5402-5404	12359721	denotes	32
22496647-14734781-98010785	7970-7972	14734781	denotes	33
22496647-11909956-98010786	8623-8625	11909956	denotes	34
22496647-15283847-98010787	15973-15975	15283847	denotes	35
22496647-19568431-98010788	15978-15980	19568431	denotes	37
22496647-10729149-98010789	16416-16418	10729149	denotes	38
22496647-10608753-98010790	16422-16424	10608753	denotes	39

pmc-enju-pas

Id	Subject	Object	Predicate	Lexical cue
T5158	11628-11631	CC	denotes	and
T21566	19028-19038	JJ	denotes	anti-NFAT5
T21565	19023-19027	IN	denotes	with
T21564	19018-19022	NN	denotes	blot
T21563	19010-19017	JJ	denotes	western
T21562	19007-19009	IN	denotes	by
T21561	18998-19006	VB	denotes	analyzed
T21560	18994-18997	CC	denotes	and
T21559	18984-18993	VB	denotes	collected
T21558	18979-18983	VB	denotes	were
T21557	18970-18978	NN	denotes	extracts
T21556	18965-18969	NN	denotes	cell
T21555	18959-18964	NNP	denotes	Whole
T21554	18953-18958	NNP	denotes	37°C.
T21553	18950-18952	IN	denotes	at
T21552	18944-18949	NN	denotes	hours
T21551	18941-18943	CD	denotes	24
T21550	18938-18940	CC	denotes	or
T21549	18936-18937	CD	denotes	8
T21548	18932-18935	IN	denotes	for
T21547	18925-18931	NN	denotes	lysate
T21546	18921-18924	NN	denotes	MTb
T21545	18915-18920	NN	denotes	µg/ml
T21544	18912-18914	CD	denotes	10
T21543	18909-18911	TO	denotes	to
T21542	18901-18908	VB	denotes	exposed
T21541	18898-18900	CC	denotes	or
T21540	18896-18897	-RRB-	denotes	)
T21539	18889-18896	NN	denotes	control
T21538	18888-18889	-LRB-	denotes	(
T21537	18878-18887	JJ	denotes	untreated
T21536	18873-18877	VB	denotes	left
T21535	18868-18872	VB	denotes	were
T21534	18862-18867	NN	denotes	cells
T21533	18856-18861	NN	denotes	THP-1
T21532	18849-18854	NN	denotes	cells
T21531	18839-18848	JJ	denotes	monocytic
T21530	18836-18838	IN	denotes	in
T21529	18829-18835	NN	denotes	levels
T21528	18821-18828	NN	denotes	protein
T21527	18815-18820	NN	denotes	NFAT5
T21526	18805-18814	VB	denotes	increases
T21525	18798-18804	NN	denotes	lysate
T21524	18794-18797	NN	denotes	MTb
T21523	18792-18793	-RRB-	denotes	)
T21522	18791-18792	NN	denotes	C
T21521	18790-18791	-LRB-	denotes	(
T21520	18782-18789	NN	denotes	figure.
T21519	18778-18781	DT	denotes	the
T21518	18775-18777	IN	denotes	of
T21517	18768-18774	NN	denotes	bottom
T21516	18764-18767	DT	denotes	the
T21515	18761-18763	IN	denotes	at
T21514	18755-18760	VB	denotes	shown
T21513	18751-18754	VB	denotes	are
T21512	18746-18750	NN	denotes	LTRs
T21511	18737-18745	NN	denotes	HIV-1Lai
T21510	18724-18736	JJ	denotes	site-mutated
T21509	18716-18723	NN	denotes	binding
T21508	18710-18715	NN	denotes	NFAT5
T21507	18706-18709	CC	denotes	and
T21506	18696-18705	JJ	denotes	wild-type
T21505	18692-18695	DT	denotes	the
T21504	18679-18691	VB	denotes	representing
T21503	18669-18678	NN	denotes	sequences
T21502	18658-18668	NN	denotes	Nucleotide
T21501	18655-18656	-RRB-	denotes	)
T21500	18651-18655	CD	denotes	0.01
T21499	18650-18651	SYM	denotes	<
T21498	18649-18650	NN	denotes	p
T21497	18647-18648	-COMMA-	denotes	,
T21496	18645-18647	SYM	denotes	**
T21495	18643-18644	-COLON-	denotes	;
T21494	18639-18643	CD	denotes	0.05
T21493	18638-18639	SYM	denotes	<
T21492	18637-18638	NN	denotes	p
T21491	18635-18636	-COMMA-	denotes	,
T21490	18634-18635	SYM	denotes	*
T21489	18633-18634	-LRB-	denotes	(
T21488	18622-18632	NN	denotes	expression
T21487	18614-18621	NN	denotes	control
T21486	18603-18613	NN	denotes	luciferase
T21485	18595-18602	NNP	denotes	Renilla
T21484	18592-18594	TO	denotes	to
T21483	18583-18591	VB	denotes	adjusted
T21482	18579-18582	CC	denotes	and
T21481	18569-18578	NN	denotes	duplicate
T21480	18566-18568	IN	denotes	in
T21479	18556-18565	VB	denotes	performed
T21478	18544-18555	NN	denotes	experiments
T21477	18532-18543	JJ	denotes	independent
T21476	18526-18531	CD	denotes	three
T21475	18521-18525	IN	denotes	from
T21474	18517-18520	VB	denotes	are
T21473	18509-18516	NN	denotes	Results
T21472	18506-18507	-RRB-	denotes	)
T21471	18502-18506	CD	denotes	0.05
T21470	18501-18502	SYM	denotes	<
T21469	18500-18501	NN	denotes	p
T21468	18499-18500	-LRB-	denotes	(
T21467	18492-18498	NN	denotes	extent
T21466	18485-18491	JJ	denotes	lesser
T21465	18483-18484	DT	denotes	a
T21464	18480-18482	TO	denotes	to
T21463	18476-18479	CC	denotes	but
T21462	18470-18475	NN	denotes	cells
T21461	18460-18469	JJ	denotes	untreated
T21460	18456-18459	DT	denotes	the
T21459	18453-18455	IN	denotes	in
T21458	18442-18452	VB	denotes	suppressed
T21457	18437-18441	RB	denotes	also
T21456	18433-18436	VB	denotes	was
T21455	18424-18432	NN	denotes	activity
T21454	18420-18423	NN	denotes	LTR
T21453	18417-18418	-RRB-	denotes	)
T21452	18413-18417	CD	denotes	0.01
T21451	18412-18413	SYM	denotes	<
T21450	18411-18412	NN	denotes	p
T21449	18410-18411	-LRB-	denotes	(
T21448	18403-18409	NN	denotes	lysate
T21447	18399-18402	NN	denotes	MTb
T21446	18394-18398	IN	denotes	with
T21445	18386-18393	VB	denotes	treated
T21444	18381-18385	VB	denotes	were
T21443	18375-18380	NN	denotes	cells
T21442	18370-18374	WRB	denotes	when
T21441	18366-18369	NN	denotes	LTR
T21440	18356-18365	JJ	denotes	wild-type
T21439	18352-18355	DT	denotes	the
T21438	18349-18351	TO	denotes	to
T21437	18338-18348	NN	denotes	comparison
T21436	18335-18337	IN	denotes	in
T21435	18324-18334	NN	denotes	expression
T21434	18319-18323	NN	denotes	gene
T21433	18310-18318	NN	denotes	reporter
T21432	18299-18309	JJ	denotes	LTR-driven
T21431	18288-18298	VB	denotes	suppressed
T21430	18274-18287	RB	denotes	significantly
T21429	18267-18273	NN	denotes	region
T21428	18258-18266	NN	denotes	enhancer
T21427	18254-18257	DT	denotes	the
T21426	18251-18253	TO	denotes	to
T21425	18243-18250	NN	denotes	binding
T21424	18237-18242	NN	denotes	NFAT5
T21423	18234-18236	IN	denotes	of
T21422	18223-18233	NNP	denotes	Disruption
T21421	18217-18222	NNP	denotes	37°C.
T21420	18214-18216	IN	denotes	at
T21419	18208-18213	NN	denotes	hours
T21418	18206-18207	CD	denotes	8
T21417	18202-18205	IN	denotes	for
T21416	18195-18201	NN	denotes	lysate
T21415	18191-18194	NN	denotes	MTb
T21414	18185-18190	NN	denotes	µg/ml
T21413	18182-18184	CD	denotes	10
T21412	18179-18181	TO	denotes	to
T21411	18171-18178	VB	denotes	exposed
T21410	18168-18170	CC	denotes	or
T21409	18158-18167	JJ	denotes	untreated
T21408	18153-18157	VB	denotes	left
T21407	18148-18152	VB	denotes	were
T21406	18142-18147	NN	denotes	cells
T21405	18138-18141	DT	denotes	the
T21404	18136-18137	-COMMA-	denotes	,
T21403	18131-18136	NN	denotes	hours
T21402	18128-18130	CD	denotes	16
T21401	18122-18127	IN	denotes	After
T21400	18119-18120	-RRB-	denotes	)
T21399	18113-18119	NN	denotes	N5-Mut
T21398	18112-18113	-LRB-	denotes	(
T21397	18102-18111	NN	denotes	mutations
T21396	18097-18101	NN	denotes	site
T21395	18089-18096	NN	denotes	binding
T21394	18083-18088	NN	denotes	NFAT5
T21393	18079-18082	DT	denotes	the
T21392	18068-18078	VB	denotes	containing
T21391	18064-18067	NN	denotes	LTR
T21390	18055-18063	NN	denotes	HIV-1Lai
T21389	18052-18054	DT	denotes	an
T21388	18048-18051	CC	denotes	and
T21387	18044-18047	NN	denotes	LTR
T21386	18035-18043	NN	denotes	HIV-1Lai
T21385	18025-18034	JJ	denotes	wild-type
T21384	18021-18024	DT	denotes	the
T21383	18018-18020	IN	denotes	of
T21382	18014-18017	CD	denotes	+64
T21360	17845-17853	NN	denotes	porter g
T21359	17841-17845	NN	denotes	R-re
T21358	17829-17841	NN	denotes	y reduces LT
T21357	17822-17829	VB	denotes	ficantl
T21356	17809-17822	RB	denotes	ng site signi
T21355	17805-17809	NN	denotes	indi
T21354	17798-17805	NN	denotes	NFAT5 b
T21353	17792-17797	NN	denotes	f the
T21352	17789-17792	DT	denotes	n o
T21351	17787-17789	IN	denotes	io
T21350	17777-17787	NN	denotes	ic disrupt
T21349	17769-17777	JJ	denotes	) Specif
T21348	17768-17769	-RRB-	denotes	B
T21347	17767-17768	NN	denotes	(
T21346	17765-17766	-LRB-	denotes	.
T21345	17764-17765	-RRB-	denotes	)
T21344	17756-17764	NN	denotes	cultures
T21343	17743-17755	JJ	denotes	unstimulated
T21342	17740-17742	TO	denotes	to
T21341	17731-17739	VB	denotes	compared
T21340	17728-17730	IN	denotes	as
T21339	17723-17727	CD	denotes	0.01
T21338	17722-17723	SYM	denotes	<
T21337	17721-17722	NN	denotes	p
T21336	17719-17720	-COMMA-	denotes	,
T21335	17717-17719	SYM	denotes	**
T21334	17715-17716	-COLON-	denotes	;
T21333	17711-17715	CD	denotes	0.05
T21332	17710-17711	SYM	denotes	<
T21331	17709-17710	NN	denotes	p
T21330	17707-17708	-COMMA-	denotes	,
T21329	17706-17707	SYM	denotes	*
T21328	17705-17706	-LRB-	denotes	(
T21327	17695-17704	NN	denotes	duplicate
T21326	17692-17694	IN	denotes	in
T21325	17682-17691	VB	denotes	performed
T21324	17670-17681	NN	denotes	experiments
T21323	17658-17669	JJ	denotes	independent
T21322	17652-17657	CD	denotes	three
T21321	17647-17651	IN	denotes	from
T21320	17643-17646	VB	denotes	are
T21319	17635-17642	NN	denotes	Results
T21318	17625-17633	NN	denotes	cultures
T21317	17615-17624	JJ	denotes	untreated
T21316	17612-17614	TO	denotes	to
T21315	17601-17611	NN	denotes	comparison
T21314	17598-17600	IN	denotes	in
T21313	17591-17597	NN	denotes	lysate
T21312	17587-17590	NN	denotes	MTb
T21311	17582-17586	IN	denotes	with
T21310	17574-17581	VB	denotes	treated
T21309	17565-17573	NN	denotes	cultures
T21308	17562-17564	IN	denotes	in
T21307	17552-17561	VB	denotes	increased
T21306	17538-17551	RB	denotes	significantly
T21305	17534-17537	VB	denotes	was
T21304	17527-17533	VB	denotes	tested
T21303	17522-17526	NN	denotes	LTRs
T21302	17507-17521	JJ	denotes	representative
T21301	17503-17506	DT	denotes	the
T21300	17500-17502	IN	denotes	of
T21299	17496-17499	DT	denotes	all
T21298	17492-17495	IN	denotes	for
T21297	17483-17491	NN	denotes	activity
T21296	17467-17482	JJ	denotes	transcriptional
T21295	17463-17466	NN	denotes	LTR
T21294	17455-17461	NN	denotes	lysate
T21293	17451-17454	NN	denotes	MTb
T21292	17446-17450	IN	denotes	with
T21291	17438-17445	VB	denotes	treated
T21290	17432-17437	NN	denotes	cells
T21289	17429-17431	IN	denotes	in
T21288	17421-17428	NN	denotes	subtype
T21287	17416-17420	DT	denotes	each
T21286	17411-17415	IN	denotes	from
T21285	17400-17410	NN	denotes	activities
T21284	17396-17399	NN	denotes	LTR
T21283	17393-17395	IN	denotes	of
T21282	17386-17392	NN	denotes	values
T21281	17381-17385	JJ	denotes	mean
T21280	17371-17380	VB	denotes	represent
T21279	17366-17370	NN	denotes	bars
T21278	17361-17365	JJ	denotes	grey
T21277	17356-17360	JJ	denotes	dark
T21276	17352-17355	CC	denotes	and
T21275	17350-17351	-COMMA-	denotes	,
T21274	17345-17350	NN	denotes	cells
T21273	17330-17344	JJ	denotes	lysate-treated
T21272	17326-17329	NN	denotes	MTb
T21271	17323-17325	IN	denotes	in
T21270	17312-17322	NN	denotes	activities
T21269	17308-17311	NN	denotes	LTR
T21268	17297-17307	JJ	denotes	individual
T21267	17287-17296	VB	denotes	represent
T21266	17282-17286	NN	denotes	bars
T21265	17276-17281	JJ	denotes	Black
T21264	17269-17274	NN	denotes	cells
T21263	17259-17268	JJ	denotes	untreated
T21262	17256-17258	IN	denotes	in
T21261	17248-17255	NN	denotes	subtype
T21260	17243-17247	DT	denotes	each
T21259	17238-17242	IN	denotes	from
T21258	17227-17237	NN	denotes	activities
T21257	17223-17226	NN	denotes	LTR
T21256	17220-17222	IN	denotes	of
T21255	17213-17219	NN	denotes	values
T21254	17208-17212	JJ	denotes	mean
T21253	17198-17207	VB	denotes	represent
T21252	17193-17197	NN	denotes	bars
T21251	17188-17192	JJ	denotes	grey
T21250	17182-17187	NN	denotes	Light
T21249	17175-17180	NN	denotes	cells
T21248	17165-17174	JJ	denotes	untreated
T21247	17162-17164	IN	denotes	in
T21246	17151-17161	NN	denotes	activities
T21245	17147-17150	NN	denotes	LTR
T21244	17136-17146	JJ	denotes	individual
T21243	17126-17135	VB	denotes	represent
T21242	17121-17125	NN	denotes	bars
T21241	17116-17120	JJ	denotes	open
T21240	17114-17115	-COMMA-	denotes	,
T21239	17105-17114	NN	denotes	histogram
T21238	17101-17104	DT	denotes	the
T21237	17098-17100	IN	denotes	In
T21236	17088-17096	NN	denotes	cultures
T21235	17084-17087	DT	denotes	the
T21234	17081-17083	IN	denotes	of
T21233	17069-17080	NN	denotes	termination
T21232	17062-17068	IN	denotes	before
T21231	17056-17061	NN	denotes	hours
T21230	17054-17055	CD	denotes	8
T21229	17050-17053	IN	denotes	for
T21228	17043-17049	NN	denotes	lysate
T21227	17039-17042	NN	denotes	MTb
T21226	17033-17038	NN	denotes	µg/ml
T21225	17030-17032	CD	denotes	10
T21224	17025-17029	IN	denotes	with
T21223	17017-17024	VB	denotes	treated
T21222	17014-17016	CC	denotes	or
T21221	17004-17013	JJ	denotes	untreated
T21220	16999-17003	JJ	denotes	left
T21219	16992-16998	CC	denotes	either
T21218	16987-16991	RB	denotes	then
T21217	16982-16986	VB	denotes	were
T21216	16976-16981	NN	denotes	Cells
T21215	16969-16974	NN	denotes	hours
T21214	16966-16968	CD	denotes	16
T21213	16962-16965	IN	denotes	for
T21212	16957-16961	NN	denotes	37°C
T21211	16954-16956	IN	denotes	at
T21210	16944-16953	VB	denotes	incubated
T21209	16940-16943	CC	denotes	and
T21208	16938-16939	-RRB-	denotes	)
T21207	16933-16938	NN	denotes	µg/ml
T21206	16928-16932	CD	denotes	0.03
T21205	16927-16928	-LRB-	denotes	(
T21204	16920-16926	NN	denotes	pRL-TK
T21203	16912-16919	NN	denotes	plasmid
T21202	16904-16911	NN	denotes	control
T21201	16893-16903	NN	denotes	luciferase
T21200	16885-16892	NNP	denotes	Renilla
T21199	16881-16884	DT	denotes	the
T21198	16876-16880	CC	denotes	plus
T21197	16874-16875	-RRB-	denotes	)
T21196	16869-16874	NN	denotes	µg/ml
T21195	16865-16868	CD	denotes	0.3
T21194	16864-16865	-LRB-	denotes	(
T21193	16856-16863	NN	denotes	plasmid
T21192	16847-16855	NN	denotes	reporter
T21191	16842-16846	DT	denotes	each
T21190	16837-16841	IN	denotes	with
T21189	16825-16836	VB	denotes	transfected
T21188	16820-16824	VB	denotes	were
T21187	16818-16819	-RRB-	denotes	)
T21186	16808-16818	NN	denotes	0.8×106/ml
T21185	16807-16808	-LRB-	denotes	(
T21184	16801-16806	NN	denotes	cells
T21183	16795-16800	NN	denotes	THP-1
T21182	16789-16793	NN	denotes	pGL3
T21181	16781-16788	NN	denotes	plasmid
T21180	16776-16780	IN	denotes	into
T21179	16769-16775	VB	denotes	cloned
T21178	16764-16768	VB	denotes	were
T21177	16755-16763	NN	denotes	isolates
T21176	16749-16754	JJ	denotes	viral
T21175	16747-16748	NN	denotes	E
T21174	16743-16746	CC	denotes	and
T21173	16741-16742	-COMMA-	denotes	,
T21172	16740-16741	NN	denotes	C
T21171	16738-16739	-COMMA-	denotes	,
T21170	16737-16738	NN	denotes	B
T21169	16729-16736	NN	denotes	subtype
T21168	16714-16728	JJ	denotes	representative
T21167	16709-16713	IN	denotes	from
T21166	16707-16708	-RRB-	denotes	)
T21165	16703-16707	NN	denotes	site
T21164	16697-16702	NN	denotes	start
T21163	16683-16696	NN	denotes	transcription
T21162	16679-16682	DT	denotes	the
T21161	16676-16678	TO	denotes	to
T21160	16667-16675	JJ	denotes	relative
T21159	16664-16666	NN	denotes	nt
T21158	16660-16663	CD	denotes	+64
T21157	16657-16659	TO	denotes	to
T21156	16652-16656	CD	denotes	−208
T21155	16651-16652	-LRB-	denotes	(
T21154	16646-16650	NN	denotes	LTRs
T21153	16640-16645	NN	denotes	HIV-1
T21152	16637-16639	NNP	denotes	E.
T21151	16633-16636	CC	denotes	and
T21150	16631-16632	-COMMA-	denotes	,
T21149	16630-16631	NN	denotes	C
T21148	16628-16629	-COMMA-	denotes	,
T21147	16627-16628	NN	denotes	B
T21146	16618-16626	NN	denotes	subtypes
T21145	16612-16617	NN	denotes	HIV-1
T21142	16594-16598	NN	denotes	LTRs
T21141	16591-16593	IN	denotes	of
T21140	16582-16590	NN	denotes	activity
T21139	16572-16581	VB	denotes	increases
T21138	16560-16571	NN	denotes	stimulation
T21137	16556-16559	NN	denotes	MTb
T21136	16554-16555	-RRB-	denotes	)
T21135	16553-16554	NN	denotes	A
T21134	16552-16553	-LRB-	denotes	(
T21133	16537-16550	NN	denotes	transcription
T21132	16531-16536	NN	denotes	HIV-1
T21131	16519-16530	JJ	denotes	MTb-induced
T21130	16515-16518	IN	denotes	for
T21129	16505-16514	JJ	denotes	important
T21128	16502-16504	VB	denotes	is
T21127	16498-16501	NN	denotes	LTR
T21126	16494-16497	DT	denotes	the
T21125	16489-16493	IN	denotes	with
T21124	16477-16488	NN	denotes	interaction
T21123	16471-16476	NN	denotes	NFAT5
T21381	18011-18013	TO	denotes	to
T21380	18007-18010	CD	denotes	208
T21379	17995-18006	NN	denotes	nucleotides
T21378	17986-17994	VB	denotes	encoding
T21377	17978-17985	NN	denotes	vectors
T21376	17967-17977	NN	denotes	expression
T21375	17956-17966	NN	denotes	luciferase
T21374	17951-17955	IN	denotes	with
T21373	17939-17950	VB	denotes	transfected
T21372	17934-17938	VB	denotes	were
T21371	17928-17933	NN	denotes	cells
T21370	17922-17927	NN	denotes	THP-1
T21369	17891-17900	NN	denotes	ponse to
T21368	17885-17891	NN	denotes	in res
T21367	17881-17884	NN	denotes	lls
T21366	17879-17881	TO	denotes	ce
T21365	17871-17879	NN	denotes	nocytic
T21364	17869-17871	IN	denotes	mo
T21363	17864-17869	NN	denotes	y in
T21362	17855-17864	JJ	denotes	e activit
T21361	17853-17855	IN	denotes	en
T21144	16607-16611	IN	denotes	from
T21143	16599-16606	VB	denotes	derived
T6993	16424-16425	-RRB-	denotes	]
T6992	16422-16424	CD	denotes	39
T6991	16421-16422	-LRB-	denotes	[
T6990	16419-16420	-COMMA-	denotes	,
T6989	16418-16419	-RRB-	denotes	]
T6988	16416-16418	CD	denotes	38
T6987	16415-16416	-LRB-	denotes	[
T6986	16406-16414	NN	denotes	stimulus
T6985	16404-16405	DT	denotes	a
T6984	16401-16403	IN	denotes	as
T6983	16397-16400	NN	denotes	TNF
T6982	16392-16396	VB	denotes	used
T6981	16387-16391	WDT	denotes	that
T6980	16378-16386	NN	denotes	activity
T6979	16374-16377	NN	denotes	LTR
T6978	16357-16373	JJ	denotes	subtype-specific
T6977	16343-16356	VB	denotes	demonstrating
T6976	16335-16342	NN	denotes	studies
T6975	16326-16334	JJ	denotes	previous
T6974	16321-16325	IN	denotes	with
T6973	16310-16320	JJ	denotes	consistent
T6972	16308-16309	-COMMA-	denotes	,
T6971	16307-16308	-RRB-	denotes	)
T6970	16305-16307	NN	denotes	1A
T6969	16298-16304	NN	denotes	Figure
T6968	16297-16298	-LRB-	denotes	(
T6967	16288-16296	NN	denotes	activity
T6966	16280-16287	JJ	denotes	weakest
T6965	16276-16279	DT	denotes	the
T6964	16269-16275	VB	denotes	showed
T6963	16256-16268	RB	denotes	consistently
T6962	16247-16255	NN	denotes	isolates
T6961	16245-16246	NN	denotes	E
T6960	16237-16244	NN	denotes	subtype
T6959	16232-16236	IN	denotes	from
T6958	16227-16231	NN	denotes	LTRs
T6957	16223-16226	DT	denotes	the
T6956	16217-16222	IN	denotes	while
T6955	16215-16216	-COMMA-	denotes	,
T6954	16207-16215	NN	denotes	activity
T6953	16197-16206	JJ	denotes	strongest
T6952	16193-16196	DT	denotes	the
T6951	16183-16192	VB	denotes	displayed
T6950	16178-16182	NN	denotes	LTRs
T6949	16176-16177	NN	denotes	C
T6948	16168-16175	NN	denotes	Subtype
T6947	16150-16166	JJ	denotes	subtype-specific
T6946	16146-16149	VB	denotes	was
T6945	16139-16145	NN	denotes	effect
T6944	16134-16138	DT	denotes	this
T6943	16131-16133	IN	denotes	of
T6942	16121-16130	NN	denotes	magnitude
T6941	16117-16120	DT	denotes	the
T6940	16113-16116	CC	denotes	and
T6939	16111-16112	-RRB-	denotes	)
T6938	16109-16111	NN	denotes	1A
T6937	16102-16108	NN	denotes	Figure
T6936	16101-16102	-LRB-	denotes	(
T6935	16092-16100	NN	denotes	activity
T6934	16081-16091	NN	denotes	luciferase
T6933	16078-16080	IN	denotes	in
T6932	16066-16077	NN	denotes	enhancement
T6931	16054-16065	JJ	denotes	significant
T6930	16052-16053	DT	denotes	a
T6929	16039-16051	VB	denotes	demonstrated
T6928	16029-16038	NN	denotes	reporters
T6927	16018-16028	JJ	denotes	LTR-driven
T6926	16016-16017	NN	denotes	E
T6925	16012-16015	CC	denotes	and
T6924	16010-16011	-COMMA-	denotes	,
T6691	15175-15178	NN	denotes	SEM
T6690	15173-15174	NN	denotes	±
T6689	15168-15172	JJ	denotes	mean
T6688	15165-15167	IN	denotes	as
T6687	15155-15164	VB	denotes	expressed
T6686	15151-15154	VB	denotes	are
T6685	15143-15150	NN	denotes	results
T6684	15141-15142	-COMMA-	denotes	,
T6683	15131-15141	JJ	denotes	applicable
T6682	15125-15130	WRB	denotes	Where
T6681	15116-15124	NN	denotes	analysis
T6680	15104-15115	JJ	denotes	Statistical
T6592	15095-15101	NN	denotes	values
T6591	15086-15094	JJ	denotes	relative
T6590	15083-15085	IN	denotes	as
T6589	15073-15082	VB	denotes	expressed
T6588	15069-15072	CC	denotes	and
T6587	15061-15068	NN	denotes	control
T6586	15052-15060	JJ	denotes	internal
T6585	15049-15051	DT	denotes	an
T6584	15046-15048	IN	denotes	as
T6583	15041-15045	NN	denotes	mRNA
T6582	15033-15040	NN	denotes	β-actin
T6581	15027-15032	VB	denotes	using
T6580	15016-15026	VB	denotes	normalized
T6579	15011-15015	VB	denotes	were
T6578	15003-15010	NN	denotes	results
T6577	14999-15002	DT	denotes	The
T6576	14994-14997	NN	denotes	min
T6575	14992-14993	CD	denotes	1
T6574	14988-14991	IN	denotes	for
T6573	14983-14987	NN	denotes	60°C
T6572	14979-14982	CC	denotes	and
T6571	14975-14978	NN	denotes	sec
T6570	14972-14974	CD	denotes	15
T6569	14968-14971	IN	denotes	for
T6568	14963-14967	NN	denotes	95°C
T6567	14960-14962	IN	denotes	of
T6566	14953-14959	NN	denotes	cycles
T6565	14950-14952	CD	denotes	40
T6564	14947-14949	IN	denotes	by
T6563	14938-14946	VB	denotes	followed
T6546	14818-14829	JJ	denotes	Green-based
T6545	14813-14817	NN	denotes	SYBR
T6544	14810-14812	IN	denotes	by
T6543	14799-14809	VB	denotes	determined
T6542	14794-14798	VB	denotes	were
T6541	14787-14793	NN	denotes	levels
T6540	14776-14786	NN	denotes	expression
T6539	14771-14775	NN	denotes	mRNA
T6538	14767-14770	DT	denotes	The
T6537	14763-14766	NN	denotes	PCR
T6536	14750-14762	JJ	denotes	Quantitative
T6923	16009-16010	NN	denotes	C
T6922	16007-16008	-COMMA-	denotes	,
T6921	16006-16007	NN	denotes	B
T6920	16002-16005	DT	denotes	the
T6919	16000-16001	-COMMA-	denotes	,
T6918	15989-16000	NN	denotes	stimulation
T6917	15984-15988	IN	denotes	Upon
T6916	15981-15982	-RRB-	denotes	)
T6915	15980-15981	-RRB-	denotes	]
T6914	15978-15980	CD	denotes	37
T6913	15977-15978	-LRB-	denotes	[
T6912	15976-15977	NN	denotes	–
T6911	15975-15976	-RRB-	denotes	]
T6910	15973-15975	CD	denotes	35
T6909	15972-15973	-LRB-	denotes	[
T6908	15970-15971	-COMMA-	denotes	,
T6907	15963-15970	NN	denotes	example
T6906	15959-15962	IN	denotes	for
T6905	15955-15958	VB	denotes	see
T6904	15954-15955	-LRB-	denotes	(
T6903	15950-15953	NN	denotes	MTb
T6902	15945-15949	VB	denotes	live
T6901	15942-15944	TO	denotes	to
T6900	15933-15941	NN	denotes	response
T6899	15930-15932	IN	denotes	in
T6898	15922-15929	VB	denotes	induced
T6897	15916-15921	DT	denotes	those
T6896	15907-15915	VB	denotes	resemble
T6895	15902-15906	WDT	denotes	that
T6894	15892-15901	NN	denotes	monocytes
T6893	15889-15891	IN	denotes	in
T6892	15879-15888	NN	denotes	responses
T6891	15866-15878	JJ	denotes	inflammatory
T6890	15858-15865	VB	denotes	induces
T6889	15851-15857	NN	denotes	lysate
T6888	15847-15850	NN	denotes	MTb
T6887	15842-15846	IN	denotes	that
T6886	15837-15841	VB	denotes	note
T6885	15834-15836	PRP	denotes	We
T6884	15831-15832	-RRB-	denotes	)
T6883	15826-15831	NN	denotes	H37Rv
T6882	15825-15826	-LRB-	denotes	(
T6881	15821-15824	NN	denotes	MTb
T6880	15818-15820	IN	denotes	of
T6879	15811-15817	NN	denotes	lysate
T6878	15806-15810	NN	denotes	cell
T6877	15800-15805	JJ	denotes	whole
T6876	15789-15799	JJ	denotes	irradiated
T6875	15786-15788	DT	denotes	an
T6874	15781-15785	IN	denotes	with
T6873	15770-15780	VB	denotes	stimulated
T6872	15765-15769	VB	denotes	were
T6871	15759-15764	NN	denotes	cells
T6870	15757-15758	-COMMA-	denotes	,
T6869	15749-15757	NN	denotes	plasmids
T6868	15743-15748	DT	denotes	these
T6867	15738-15742	IN	denotes	with
T6866	15733-15737	NN	denotes	line
T6865	15728-15732	NN	denotes	cell
T6864	15722-15727	NN	denotes	THP-1
T6863	15712-15721	JJ	denotes	monocytic
T6862	15708-15711	DT	denotes	the
T6861	15705-15707	IN	denotes	of
T6860	15692-15704	NN	denotes	transfection
T6859	15686-15691	IN	denotes	After
T6858	15680-15684	NN	denotes	gene
T6857	15671-15679	NN	denotes	reporter
T6856	15660-15670	NN	denotes	luciferase
T6855	15652-15659	NN	denotes	firefly
T6854	15648-15651	DT	denotes	the
T6853	15645-15647	TO	denotes	to
T6852	15638-15644	VB	denotes	linked
T6851	15636-15637	-RRB-	denotes	)
T6850	15632-15636	NN	denotes	site
T6849	15626-15631	NN	denotes	start
T6848	15612-15625	NN	denotes	transcription
T6847	15608-15611	DT	denotes	the
T6846	15605-15607	TO	denotes	to
T6845	15596-15604	JJ	denotes	relative
T6844	15593-15595	NN	denotes	nt
T6843	15590-15592	CD	denotes	64
T6842	15588-15589	SYM	denotes	+
T6841	15585-15587	TO	denotes	to
T6840	15580-15584	CD	denotes	−208
T6839	15579-15580	-LRB-	denotes	(
T6838	15574-15578	NN	denotes	LTRs
T6837	15572-15573	NN	denotes	E
T6836	15568-15571	CC	denotes	and
T6835	15566-15567	-COMMA-	denotes	,
T6834	15565-15566	NN	denotes	C
T6833	15563-15564	-COMMA-	denotes	,
T6832	15562-15563	NN	denotes	B
T6831	15554-15561	NN	denotes	subtype
T6830	15548-15553	JJ	denotes	viral
T6829	15537-15547	VB	denotes	containing
T6828	15528-15536	NN	denotes	plasmids
T6827	15519-15527	NN	denotes	reporter
T6826	15507-15518	VB	denotes	constructed
T6825	15501-15506	RB	denotes	first
T6824	15498-15500	PRP	denotes	we
T6823	15496-15497	-COMMA-	denotes	,
T6822	15488-15496	NN	denotes	activity
T6821	15484-15487	NN	denotes	LTR
T6820	15478-15483	NN	denotes	HIV-1
T6819	15461-15477	JJ	denotes	subtype-specific
T6818	15458-15460	IN	denotes	on
T6817	15446-15457	NN	denotes	stimulation
T6816	15442-15445	NN	denotes	MTb
T6815	15439-15441	IN	denotes	of
T6814	15432-15438	NN	denotes	impact
T6813	15421-15431	JJ	denotes	functional
T6812	15417-15420	DT	denotes	the
T6811	15409-15416	VB	denotes	compare
T6810	15406-15408	TO	denotes	To
T6809	15404-15405	NN	denotes	E
T6808	15400-15403	CC	denotes	and
T6807	15398-15399	-COMMA-	denotes	,
T6806	15397-15398	NN	denotes	C
T6805	15395-15396	-COMMA-	denotes	,
T6804	15394-15395	NN	denotes	B
T6803	15385-15393	NN	denotes	subtypes
T6802	15379-15384	NN	denotes	HIV-1
T6801	15376-15378	IN	denotes	of
T6800	15367-15375	NN	denotes	activity
T6799	15363-15366	NN	denotes	LTR
T6798	15357-15362	NN	denotes	HIV-1
T6797	15347-15356	VB	denotes	increases
T6796	15343-15346	NN	denotes	MTb
T6234	14746-14747	-RRB-	denotes	)
T6233	14740-14746	NNP	denotes	Pierce
T6232	14739-14740	-LRB-	denotes	(
T6231	14731-14738	NNP	denotes	Reagent
T6230	14714-14730	NNP	denotes	Chemiluminescent
T6229	14709-14713	NNP	denotes	Pico
T6228	14704-14708	NNP	denotes	West
T6227	14692-14703	NNP	denotes	SuperSignal
T6226	14687-14691	IN	denotes	with
T6225	14677-14686	VB	denotes	developed
T6224	14673-14676	CC	denotes	and
T6223	14668-14672	NN	denotes	TBST
T6222	14665-14667	IN	denotes	in
T6221	14661-14664	NN	denotes	min
T6220	14657-14660	CD	denotes	3×5
T6219	14650-14656	VB	denotes	washed
T6218	14644-14649	RB	denotes	again
T6217	14640-14643	VB	denotes	was
T6216	14635-14639	NN	denotes	blot
T6215	14631-14634	DT	denotes	The
T6214	14628-14629	NN	denotes	h
T6213	14626-14627	CD	denotes	1
T6212	14622-14625	IN	denotes	for
T6211	14610-14621	JJ	denotes	appropriate
T6210	14607-14609	IN	denotes	as
T6209	14605-14606	-RRB-	denotes	)
T6208	14599-14605	NN	denotes	BioRad
T6207	14598-14599	-LRB-	denotes	(
T6206	14582-14597	NN	denotes	anti-rabbit-HRP
T6205	14577-14581	NN	denotes	goat
T6204	14574-14576	CC	denotes	or
T6203	14572-14573	-RRB-	denotes	)
T6202	14559-14572	NNP	denotes	Biotechnology
T6201	14554-14558	NNP	denotes	Cruz
T6200	14548-14553	NNP	denotes	Santa
T6199	14547-14548	-LRB-	denotes	(
T6198	14533-14546	NN	denotes	anti-goat-HRP
T6197	14526-14532	NN	denotes	donkey
T6196	14519-14525	CD	denotes	1∶6000
T6195	14516-14518	IN	denotes	in
T6194	14506-14515	VB	denotes	incubated
T6193	14502-14505	CC	denotes	and
T6192	14497-14501	NN	denotes	TBST
T6191	14494-14496	IN	denotes	in
T6190	14490-14493	NN	denotes	min
T6189	14486-14489	CD	denotes	3×5
T6188	14479-14485	VB	denotes	washed
T6187	14475-14478	VB	denotes	was
T6186	14470-14474	NN	denotes	blot
T6185	14466-14469	DT	denotes	The
T6184	14453-14464	NN	denotes	temperature
T6183	14448-14452	NN	denotes	room
T6182	14445-14447	IN	denotes	at
T6181	14443-14444	NN	denotes	h
T6180	14441-14442	CD	denotes	2
T6179	14437-14440	IN	denotes	for
T6178	14428-14436	NN	denotes	BSA/TBST
T6177	14425-14427	IN	denotes	in
T6176	14423-14424	-RRB-	denotes	)
T6175	14410-14423	NNP	denotes	Biotechnology
T6174	14405-14409	NNP	denotes	Cruz
T6173	14399-14404	NNP	denotes	Santa
T6172	14397-14398	-COLON-	denotes	;
T6171	14390-14397	NN	denotes	sc-6217
T6170	14389-14390	-LRB-	denotes	(
T6169	14380-14388	NN	denotes	antibody
T6168	14366-14379	NN	denotes	anti-Lamin-B1
T6167	14361-14365	NN	denotes	goat
T6166	14358-14360	IN	denotes	of
T6165	14349-14357	NN	denotes	dilution
T6164	14343-14348	CD	denotes	1∶500
T6163	14341-14342	DT	denotes	a
T6162	14337-14340	CC	denotes	and
T6161	14335-14336	-RRB-	denotes	)
T6160	14322-14335	NNP	denotes	Biotechnology
T6159	14317-14321	NNP	denotes	Cruz
T6158	14311-14316	NNP	denotes	Santa
T6157	14310-14311	-LRB-	denotes	(
T6156	14308-14309	-RRB-	denotes	)
T6155	14303-14308	NN	denotes	H-300
T6154	14302-14303	-LRB-	denotes	(
T6153	14293-14301	NN	denotes	antibody
T6152	14282-14292	JJ	denotes	anti-NFAT5
T6151	14275-14281	NN	denotes	rabbit
T6150	14272-14274	IN	denotes	of
T6149	14263-14271	NN	denotes	dilution
T6148	14256-14262	NN	denotes	a1∶200
T6147	14251-14255	IN	denotes	with
T6146	14247-14250	RP	denotes	out
T6145	14239-14246	VB	denotes	carried
T6144	14235-14238	VB	denotes	was
T6143	14224-14234	NN	denotes	incubation
T6142	14216-14223	JJ	denotes	Primary
T6141	14213-14214	-RRB-	denotes	)
T6140	14205-14213	NN	denotes	BSA/TBST
T6139	14204-14205	-LRB-	denotes	(
T6562	14934-14937	NN	denotes	min
T6561	14931-14933	CD	denotes	10
T6560	14927-14930	IN	denotes	for
T6559	14922-14926	NN	denotes	95°C
T6558	14917-14921	VB	denotes	were
T6557	14906-14916	NN	denotes	conditions
T6556	14897-14905	NN	denotes	reaction
T6555	14893-14896	DT	denotes	The
T6554	14890-14891	-RRB-	denotes	)
T6553	14865-14890	NN	denotes	www.appliedbiosystems.com
T6552	14863-14864	-COMMA-	denotes	,
T6551	14853-14863	NNP	denotes	Biosystems
T6550	14845-14852	NNP	denotes	Applied
T6549	14844-14845	-LRB-	denotes	(
T6548	14840-14843	NN	denotes	PCR
T6547	14830-14839	JJ	denotes	real-time
T6138	14200-14203	CD	denotes	7.6
T6137	14197-14199	NN	denotes	pH
T6136	14194-14196	IN	denotes	at
T6135	14189-14193	NN	denotes	NaCl
T6134	14186-14188	NN	denotes	mM
T6133	14182-14185	CD	denotes	150
T6132	14178-14181	CC	denotes	and
T6131	14173-14177	NNP	denotes	Tris
T6130	14170-14172	NN	denotes	mM
T6129	14167-14169	CD	denotes	50
T6128	14156-14166	VB	denotes	containing
T6127	14149-14155	NN	denotes	buffer
T6126	14147-14148	DT	denotes	a
T6125	14144-14146	IN	denotes	in
T6124	14142-14143	-RRB-	denotes	)
T6123	14136-14142	NN	denotes	BioRad
T6122	14135-14136	-LRB-	denotes	(
T6121	14126-14134	CD	denotes	Tween-20
T6120	14124-14125	NN	denotes	%
T6119	14121-14124	CD	denotes	0.1
T6118	14117-14120	CC	denotes	and
T6117	14115-14116	-RRB-	denotes	)
T6116	14110-14115	NN	denotes	Sigma
T6115	14109-14110	-LRB-	denotes	(
T6114	14105-14108	NN	denotes	BSA
T6113	14103-14104	NN	denotes	%
T6104	14073-14074	CD	denotes	1
T6103	14069-14072	IN	denotes	for
T6102	14061-14068	VB	denotes	blocked
T6101	14056-14060	RB	denotes	then
T6100	14052-14055	VB	denotes	was
T6099	14047-14051	NN	denotes	blot
T6098	14043-14046	DT	denotes	The
T6097	14040-14041	-RRB-	denotes	)
T6096	14034-14040	NNP	denotes	BioRad
T6095	14033-14034	-LRB-	denotes	(
T6094	14024-14032	NNP	denotes	Membrane
T6093	14015-14023	NNP	denotes	Transfer
T6092	14004-14014	NNP	denotes	Trans-Blot
T6091	13989-14003	NN	denotes	nitrocellulose
T6090	13987-13988	DT	denotes	a
T6089	13984-13986	TO	denotes	to
T6088	13972-13983	VB	denotes	transferred
T6087	13968-13971	VB	denotes	was
T6086	13964-13967	NN	denotes	gel
T6085	13960-13963	DT	denotes	The
T6084	13950-13958	NN	denotes	SDS-PAGE
T6083	13947-13949	IN	denotes	by
T6082	13937-13946	VB	denotes	separated
T6081	13932-13936	VB	denotes	were
T6080	13923-13931	NN	denotes	proteins
T6079	13919-13922	CC	denotes	and
T6078	13901-13918	NN	denotes	2-mercaptoethanol
T6077	13897-13900	NN	denotes	v/v
T6076	13895-13896	NN	denotes	%
T6075	13894-13895	CD	denotes	5
T6074	13889-13893	IN	denotes	with
T6073	13882-13888	NN	denotes	buffer
T6072	13875-13881	NN	denotes	sample
T6071	13867-13874	NNP	denotes	Laemmli
T6070	13864-13866	CD	denotes	1×
T6069	13861-13863	IN	denotes	in
T6068	13857-13860	NN	denotes	min
T6067	13855-13856	CD	denotes	5
T6066	13851-13854	IN	denotes	for
T6065	13844-13850	VB	denotes	boiled
T6064	13839-13843	VB	denotes	were
T6063	13830-13838	NN	denotes	Extracts
T6062	13822-13828	NN	denotes	buffer
T6061	13819-13821	IN	denotes	of
T6060	13816-13818	NN	denotes	ml
T6059	13813-13815	CD	denotes	25
T6058	13809-13812	IN	denotes	per
T6057	13807-13808	-RRB-	denotes	)
T6056	13802-13807	NNP	denotes	Roche
T6055	13801-13802	-LRB-	denotes	(
T6054	13792-13800	NNP	denotes	Cocktail
T6053	13782-13791	NNP	denotes	Inhibitor
T6052	13773-13781	NNP	denotes	Protease
T6051	13763-13772	NNP	denotes	EDTA-free
T6050	13754-13762	NNP	denotes	Complete
T6049	13751-13753	IN	denotes	of
T6048	13744-13750	NN	denotes	tablet
T6047	13742-13743	CD	denotes	1
T6046	13738-13741	CC	denotes	and
T6045	13736-13737	-COMMA-	denotes	,
T6044	13728-13736	NN	denotes	glycerol
T6043	13726-13727	NN	denotes	%
T6042	13724-13726	CD	denotes	10
T6041	13722-13723	-COMMA-	denotes	,
T6040	13716-13722	NN	denotes	Triton
T6039	13714-13715	NN	denotes	%
T6038	13713-13714	CD	denotes	1
T6037	13711-13712	-COMMA-	denotes	,
T6036	13708-13711	CD	denotes	7.5
T6035	13705-13707	NN	denotes	pH
T6034	13703-13704	-COMMA-	denotes	,
T6033	13695-13703	NN	denotes	Tris–HCl
T6032	13692-13694	NN	denotes	mM
T6031	13689-13691	CD	denotes	50
T6030	13687-13688	-COMMA-	denotes	,
T6029	13683-13687	NN	denotes	NaCl
T6028	13680-13682	NN	denotes	mM
T6027	13676-13679	CD	denotes	150
T6026	13665-13675	VB	denotes	containing
T6025	13658-13664	NN	denotes	buffer
T6024	13652-13657	NN	denotes	lysis
T6023	13647-13651	IN	denotes	with
T6022	13637-13646	VB	denotes	collected
T6021	13632-13636	VB	denotes	were
T6020	13623-13631	NN	denotes	extracts
T6019	13618-13622	NN	denotes	cell
T6018	13612-13617	JJ	denotes	Whole
T6017	13607-13611	NN	denotes	blot
T6016	13599-13606	NN	denotes	Western
T5777	13593-13596	NN	denotes	MDM
T5776	13585-13592	JJ	denotes	primary
T5775	13582-13584	IN	denotes	of
T5774	13572-13581	NN	denotes	infection
T5773	13569-13571	IN	denotes	of
T5772	13562-13568	NN	denotes	course
T5771	13558-13561	DT	denotes	the
T5770	13553-13557	IN	denotes	over
T5769	13543-13552	VB	denotes	increased
T5768	13536-13542	NN	denotes	levels
T5767	13523-13535	NN	denotes	mycobacteria
T5766	13518-13522	IN	denotes	that
T5765	13507-13517	VB	denotes	confirming
T5764	13505-13506	-COMMA-	denotes	,
T5763	13493-13505	RB	denotes	respectively
T5762	13491-13492	-COMMA-	denotes	,
T5761	13486-13491	CD	denotes	5×104
T5760	13482-13485	CC	denotes	and
T5759	13476-13481	CD	denotes	6×103
T5758	13471-13475	VB	denotes	were
T5757	13464-13470	NN	denotes	counts
T5756	13460-13463	NNP	denotes	CFU
T5755	13452-13459	JJ	denotes	average
T5754	13448-13451	DT	denotes	the
T5753	13446-13447	CD	denotes	7
T5752	13442-13445	CC	denotes	and
T5751	13440-13441	CD	denotes	4
T5750	13435-13439	NN	denotes	days
T5749	13432-13434	IN	denotes	on
T5748	13428-13431	CC	denotes	and
T5747	13418-13427	VB	denotes	performed
T5746	13414-13417	VB	denotes	was
T5745	13405-13413	NN	denotes	analysis
T5744	13403-13404	-RRB-	denotes	)
T5743	13400-13403	NN	denotes	CFU
T5742	13399-13400	-LRB-	denotes	(
T5741	13394-13398	NN	denotes	unit
T5740	13379-13393	JJ	denotes	Colony-forming
T5739	13364-13377	NN	denotes	THP-1∶bacilli
T5738	13360-13363	CC	denotes	and
T5737	13352-13359	NN	denotes	bacilli
T5736	13347-13351	NN	denotes	MDM∶
T5735	13343-13346	CD	denotes	1∶1
T5734	13340-13342	CC	denotes	or
T5733	13332-13339	NN	denotes	bacilli
T5732	13326-13331	NN	denotes	PBMC∶
T5731	13321-13325	CD	denotes	10∶1
T5730	13318-13320	IN	denotes	at
T5729	13310-13317	NN	denotes	studies
T5728	13300-13309	NN	denotes	infection
T5727	13295-13299	NN	denotes	cell
T5726	13291-13294	IN	denotes	for
T5725	13284-13290	NN	denotes	medium
T5724	13279-13283	CD	denotes	1640
T5723	13274-13278	NN	denotes	RPMI
T5722	13269-13273	IN	denotes	with
T5721	13260-13268	NN	denotes	dilution
T5720	13252-13259	JJ	denotes	further
T5719	13245-13251	IN	denotes	before
T5718	13239-13244	NN	denotes	OD650
T5717	13236-13238	IN	denotes	of
T5716	13224-13235	NN	denotes	measurement
T5715	13221-13223	IN	denotes	by
T5714	13210-13220	VB	denotes	determined
T5713	13205-13209	VB	denotes	were
T5712	13197-13204	NN	denotes	numbers
T5711	13192-13196	NN	denotes	cell
T5710	13182-13191	JJ	denotes	Bacterial
T5709	13170-13180	NN	denotes	suspension
T5708	13165-13169	NN	denotes	cell
T5707	13158-13164	JJ	denotes	single
T5706	13156-13157	DT	denotes	a
T5705	13153-13155	IN	denotes	in
T5704	13148-13152	VB	denotes	were
T5703	13139-13147	NN	denotes	bacteria
T5702	13135-13138	DT	denotes	the
T5701	13130-13134	IN	denotes	that
T5700	13123-13129	VB	denotes	ensure
T5699	13120-13122	TO	denotes	to
T5698	13113-13119	NN	denotes	filter
T5697	13110-13112	NN	denotes	µm
T5696	13108-13109	CD	denotes	5
T5695	13106-13107	DT	denotes	a
T5694	13098-13105	IN	denotes	through
T5693	13091-13097	VB	denotes	passed
T5692	13087-13090	CC	denotes	and
T5691	13085-13086	-COMMA-	denotes	,
T5690	13082-13085	NNP	denotes	PBS
T5689	13079-13081	IN	denotes	in
T5688	13067-13078	VB	denotes	resuspended
T5687	13065-13066	-COMMA-	denotes	,
T5686	13062-13065	NNP	denotes	PBS
T5685	13057-13061	IN	denotes	with
T5684	13050-13056	VB	denotes	washed
T5683	13048-13049	-COMMA-	denotes	,
T5682	13042-13048	VB	denotes	plated
T5681	13037-13041	RB	denotes	then
T5680	13032-13036	VB	denotes	were
T5679	13023-13031	NN	denotes	Bacteria
T5678	13016-13021	NN	denotes	phase
T5677	13009-13015	NN	denotes	growth
T5676	12997-13008	JJ	denotes	logarithmic
T5675	12993-12996	DT	denotes	the
T5674	12990-12992	IN	denotes	in
T5673	12985-12989	VB	denotes	were
T5672	12980-12984	PRP	denotes	they
T5671	12975-12979	IN	denotes	that
T6112	14102-14103	CD	denotes	4
T6111	14099-14101	IN	denotes	of
T6110	14090-14098	NN	denotes	solution
T6109	14088-14089	DT	denotes	a
T6108	14085-14087	IN	denotes	in
T6107	14080-14084	NN	denotes	37°C
T6106	14077-14079	IN	denotes	at
T6105	14075-14076	NN	denotes	h
T5670	12968-12974	VB	denotes	ensure
T5669	12965-12967	TO	denotes	to
T5668	12960-12964	NN	denotes	37°C
T5667	12957-12959	IN	denotes	at
T5666	12953-12956	CD	denotes	0.4
T5665	12950-12952	IN	denotes	of
T5664	12944-12949	NN	denotes	OD650
T5663	12941-12943	DT	denotes	an
T5662	12938-12940	TO	denotes	to
T5661	12932-12937	VB	denotes	grown
T5660	12927-12931	VB	denotes	were
T5659	12918-12926	NN	denotes	cultures
T5658	12914-12917	DT	denotes	The
T5657	12911-12912	-RRB-	denotes	)
T5656	12891-12911	NN	denotes	www.sigmaaldrich.com
T5655	12889-12890	-COMMA-	denotes	,
T5654	12876-12889	JJ	denotes	Sigma-Aldrich
T5653	12875-12876	-LRB-	denotes	(
T5652	12872-12874	CD	denotes	80
T5651	12866-12871	CD	denotes	Tween
T5650	12864-12865	NN	denotes	%
T5649	12860-12864	CD	denotes	0.05
T5648	12856-12859	CC	denotes	and
T5647	12854-12855	-RRB-	denotes	)
T5646	12851-12854	NN	denotes	ADC
T5645	12850-12851	-LRB-	denotes	(
T5293	12636-12645	NN	denotes	puromycin
T5292	12630-12635	NN	denotes	µg/ml
T5291	12626-12629	CD	denotes	0.8
T5290	12621-12625	IN	denotes	with
T5289	12611-12620	NN	denotes	treatment
T5288	12608-12610	IN	denotes	by
T5287	12599-12607	VB	denotes	expanded
T5286	12595-12598	CC	denotes	and
T5285	12586-12594	VB	denotes	selected
T5284	12581-12585	VB	denotes	were
T5283	12575-12580	NN	denotes	cells
T5282	12564-12574	VB	denotes	transduced
T5281	12551-12563	RB	denotes	Successfully
T5280	12546-12549	NN	denotes	RPM
T5279	12541-12545	CD	denotes	2000
T5278	12538-12540	IN	denotes	at
T5277	12532-12537	NN	denotes	hours
T5276	12528-12531	CD	denotes	two
T5275	12524-12527	IN	denotes	for
T5274	12510-12523	NN	denotes	spinoculation
T5273	12506-12509	CC	denotes	and
T5272	12504-12505	-RRB-	denotes	)
T5271	12487-12504	NN	denotes	www.millipore.com
T5270	12485-12486	-COMMA-	denotes	,
T5269	12476-12485	NNP	denotes	Millipore
T5268	12475-12476	-LRB-	denotes	(
T5267	12465-12474	NN	denotes	polybrene
T5266	12459-12464	NN	denotes	µg/ml
T5265	12457-12458	CD	denotes	8
T5264	12454-12456	IN	denotes	of
T5263	12445-12453	NN	denotes	presence
T5262	12441-12444	DT	denotes	the
T5261	12438-12440	IN	denotes	in
T5260	12432-12437	NN	denotes	cells
T5259	12416-12431	JJ	denotes	virus-producing
T5258	12412-12415	DT	denotes	the
T5257	12407-12411	IN	denotes	from
T5256	12394-12406	NN	denotes	supernatants
T5255	12389-12393	IN	denotes	with
T5254	12383-12388	NN	denotes	cells
T5253	12379-12382	DT	denotes	the
T5252	12369-12378	VB	denotes	culturing
T5251	12366-12368	IN	denotes	by
T5250	12356-12365	NN	denotes	particles
T5157	11626-11627	-RRB-	denotes	)
T5156	11616-11626	VB	denotes	underlined
T5155	11613-11615	VB	denotes	is
T5154	11604-11612	NN	denotes	sequence
T5153	11597-11603	NN	denotes	target
T5152	11592-11596	NN	denotes	mRNA
T5151	11586-11591	NN	denotes	IRAK1
T5150	11585-11586	-LRB-	denotes	(
T5149	11580-11584	NN	denotes	mRNA
T5148	11518-11579	NN	denotes	5′-AATTCAAAAAGGAGCAGCTGTCCAGGTTTTATGAGACGAAACCTGGACAGCTGCT-3′
T5147	11511-11517	NN	denotes	primer
T5146	11503-11510	JJ	denotes	reverse
T5145	11501-11502	-COMMA-	denotes	,
T5144	11440-11501	NN	denotes	5′-CCGGAGCAGCTGTCCAGGTTTCGTCTCATAAAACCTGGACAGCTGCTCCTTTTTG-3′
T5143	11433-11439	NN	denotes	primer
T5142	11425-11432	JJ	denotes	forward
T5141	11424-11425	-LRB-	denotes	(
T5140	11418-11423	NN	denotes	IRAK1
T5139	11412-11417	JJ	denotes	human
T5138	11402-11411	VB	denotes	targeting
T5137	11396-11401	NN	denotes	shRNA
T5136	11384-11395	NN	denotes	laboratory.
T5135	11380-11383	PRP-DOLLAR-	denotes	our
T5134	11377-11379	IN	denotes	in
T5133	11367-11376	VB	denotes	validated
T5132	11363-11366	VB	denotes	was
T5131	11359-11362	CC	denotes	and
T5130	11357-11358	-RRB-	denotes	)
T5129	11335-11357	NN	denotes	www.openbiosystems.com
T5128	11334-11335	-LRB-	denotes	(
T5127	11323-11333	NNP	denotes	Biosystems
T5126	11318-11322	NNP	denotes	Open
T5125	11313-11317	IN	denotes	from
T5124	11303-11312	VB	denotes	purchased
T5123	11299-11302	VB	denotes	was
T5122	11293-11298	NN	denotes	MyD88
T5121	11287-11292	JJ	denotes	human
T5120	11277-11286	VB	denotes	targeting
T5119	11271-11276	NN	denotes	shRNA
T5118	11260-11270	VB	denotes	expressing
T5117	11253-11259	NN	denotes	pLKO.1
T5116	11245-11252	NN	denotes	plasmid
T5115	11234-11244	JJ	denotes	lentiviral
T5114	11230-11233	DT	denotes	The
T5113	11224-11229	NN	denotes	shRNA
T5112	11213-11223	VB	denotes	expressing
T5111	11207-11212	NN	denotes	cells
T5110	11201-11206	NN	denotes	THP-1
T5109	11194-11200	JJ	denotes	Stable
T5644	12842-12849	NN	denotes	complex
T5643	12833-12841	NN	denotes	dextrose
T5642	12825-12832	NN	denotes	albumin
T5641	12820-12824	IN	denotes	with
T5640	12807-12819	VB	denotes	supplemented
T5639	12805-12806	-RRB-	denotes	)
T5638	12795-12805	NN	denotes	www.bd.com
T5637	12793-12794	-COMMA-	denotes	,
T5636	12791-12793	NNP	denotes	BD
T5635	12785-12790	NNP	denotes	Difco
T5634	12784-12785	-LRB-	denotes	(
T5633	12777-12783	NN	denotes	medium
T5632	12773-12776	NN	denotes	7H9
T5631	12761-12772	NNP	denotes	Middlebrook
T5630	12758-12760	IN	denotes	of
T5629	12755-12757	NN	denotes	ml
T5628	12752-12754	CD	denotes	10
T5627	12747-12751	IN	denotes	into
T5626	12741-12746	NN	denotes	stock
T5625	12732-12740	NN	denotes	bacteria
T5624	12725-12731	VB	denotes	frozen
T5623	12722-12724	IN	denotes	of
T5622	12719-12721	NN	denotes	µl
T5621	12715-12718	CD	denotes	100
T5620	12708-12714	VB	denotes	adding
T5619	12705-12707	IN	denotes	by
T5618	12696-12704	VB	denotes	prepared
T5617	12692-12695	VB	denotes	was
T5616	12684-12691	NN	denotes	CDC1551
T5615	12677-12683	NN	denotes	strain
T5614	12668-12676	JJ	denotes	clinical
T5613	12664-12667	NN	denotes	MTb
T5612	12660-12663	DT	denotes	The
T5611	12652-12659	NN	denotes	culture
T5610	12648-12651	NN	denotes	MTb
T4814	11190-11191	-RRB-	denotes	]
T4813	11188-11190	CD	denotes	31
T4812	11187-11188	-LRB-	denotes	[
T4811	11177-11186	VB	denotes	performed
T4810	11172-11176	VB	denotes	were
T4809	11160-11171	NN	denotes	experiments
T4808	11150-11159	NN	denotes	infection
T4807	11143-11149	IN	denotes	before
T4806	11132-11142	NN	denotes	expression
T4805	11126-11131	NN	denotes	NFAT5
T4804	11123-11125	IN	denotes	of
T4803	11118-11122	RP	denotes	down
T4802	11112-11117	NN	denotes	knock
T4801	11102-11111	JJ	denotes	efficient
T4800	11098-11101	IN	denotes	for
T4799	11092-11097	NN	denotes	times
T4798	11088-11091	CD	denotes	two
T4797	11076-11087	VB	denotes	transfected
T4796	11071-11075	VB	denotes	were
T4795	11067-11070	NN	denotes	MDM
T4794	11059-11065	NN	denotes	medium
T4793	11053-11058	JJ	denotes	fresh
T4792	11050-11052	IN	denotes	in
T4791	11040-11049	VB	denotes	incubated
T4790	11036-11039	CC	denotes	and
T4789	11029-11035	VB	denotes	washed
T4788	11024-11028	VB	denotes	were
T4787	11018-11023	NN	denotes	cells
T4786	11012-11017	WDT	denotes	which
T4785	11006-11011	IN	denotes	after
T4784	10996-11005	RB	denotes	overnight
T4783	10991-10995	NN	denotes	37°C
T4782	10988-10990	IN	denotes	at
T4781	10983-10987	VB	denotes	left
T4780	10978-10982	VB	denotes	were
T4779	10969-10977	NN	denotes	cultures
T4778	10965-10968	DT	denotes	The
T4777	10958-10963	NN	denotes	serum
T4776	10955-10957	NN	denotes	AB
T4775	10949-10954	JJ	denotes	human
T4774	10932-10948	JJ	denotes	heat-inactivated
T4773	10930-10931	NN	denotes	%
T4772	10929-10930	CD	denotes	5
T4771	10924-10928	CC	denotes	plus
T4770	10917-10923	NN	denotes	medium
T4769	10902-10916	JJ	denotes	Macrophage-SFM
T4768	10899-10901	IN	denotes	in
T4767	10896-10898	NN	denotes	µl
T4766	10892-10895	CD	denotes	750
T4765	10889-10891	IN	denotes	of
T4764	10882-10888	NN	denotes	volume
T4763	10876-10881	JJ	denotes	final
T4762	10874-10875	DT	denotes	a
T4761	10871-10873	IN	denotes	in
T4760	10869-10870	-COMMA-	denotes	,
T4759	10857-10869	NN	denotes	manufacturer
T4758	10853-10856	DT	denotes	the
T4757	10850-10852	IN	denotes	by
T4756	10838-10849	VB	denotes	recommended
T4755	10835-10837	IN	denotes	as
T4754	10826-10834	VB	denotes	prepared
T4753	10824-10825	-COMMA-	denotes	,
T4752	10823-10824	-RRB-	denotes	)
T4751	10809-10823	NN	denotes	www.ambion.com
T4750	10807-10808	-COMMA-	denotes	,
T4749	10803-10807	NNP	denotes	Inc.
T4748	10796-10802	NNP	denotes	Ambion
T4747	10795-10796	-LRB-	denotes	(
T4746	10787-10794	NN	denotes	reagent
T4745	10774-10786	NN	denotes	transfection
T4744	10768-10773	NN	denotes	NeoFX
T4743	10761-10767	NN	denotes	siPORT
T4742	10758-10760	IN	denotes	in
T4741	10752-10757	NN	denotes	siRNA
T4740	10742-10751	VB	denotes	indicated
T4739	10738-10741	DT	denotes	the
T4738	10735-10737	IN	denotes	of
T4737	10732-10734	NN	denotes	µM
T4736	10730-10731	CD	denotes	1
T4735	10724-10729	VB	denotes	using
T4734	10717-10723	NN	denotes	plates
T4733	10710-10716	JJ	denotes	6-well
T4732	10707-10709	IN	denotes	in
T4731	10695-10706	VB	denotes	transfected
T4730	10690-10694	VB	denotes	were
T4729	10686-10689	NN	denotes	MDM
T4728	10660-10684	NN	denotes	GGCTACGTCCAGGAGCGCACC-3′
T4727	10656-10659	JJ	denotes	5′-
T4726	10654-10655	-COMMA-	denotes	,
T4725	10653-10654	-RRB-	denotes	)
T4724	10650-10653	NN	denotes	GFP
T4723	10649-10650	-LRB-	denotes	(
T4722	10641-10648	NN	denotes	protein
T4721	10629-10640	JJ	denotes	fluorescent
T4720	10623-10628	JJ	denotes	green
T4719	10619-10622	DT	denotes	the
T4718	10609-10618	VB	denotes	targeting
T4717	10603-10608	NN	denotes	siRNA
T4716	10600-10602	DT	denotes	an
T4715	10595-10599	VB	denotes	used
T4714	10592-10594	PRP	denotes	we
T4713	10590-10591	-COMMA-	denotes	,
T4712	10583-10590	NN	denotes	effects
T4711	10577-10582	NN	denotes	siRNA
T4710	10564-10576	JJ	denotes	non-specific
T4709	10560-10563	IN	denotes	for
T4708	10552-10559	NN	denotes	control
T4707	10550-10551	DT	denotes	a
T4706	10548-10549	-COMMA-	denotes	,
T4705	10539-10548	VB	denotes	described
T4704	10536-10538	IN	denotes	As
T4703	10533-10534	-RRB-	denotes	]
T4702	10531-10533	CD	denotes	31
T4701	10530-10531	-LRB-	denotes	[
T4700	10528-10529	-RRB-	denotes	)
T4699	10525-10528	CD	denotes	353
T5249	12345-12355	JJ	denotes	lentiviral
T5248	12341-12344	DT	denotes	the
T5247	12336-12340	IN	denotes	with
T5246	12325-12335	VB	denotes	transduced
T5245	12320-12324	VB	denotes	were
T5244	12314-12319	NN	denotes	cells
T5243	12308-12313	NN	denotes	THP-1
T5242	12301-12307	NN	denotes	−80°C.
T5241	12298-12300	IN	denotes	at
T5240	12291-12297	VB	denotes	stored
T5239	12287-12290	CC	denotes	and
T5238	12285-12286	-COMMA-	denotes	,
T5237	12271-12285	NN	denotes	centrifugation
T5236	12268-12270	IN	denotes	by
T5235	12258-12267	VB	denotes	clarified
T5234	12256-12257	-COMMA-	denotes	,
T5233	12239-12256	JJ	denotes	post-transfection
T5232	12233-12238	NN	denotes	hours
T5231	12230-12232	CD	denotes	48
T5230	12220-12229	VB	denotes	collected
T5229	12215-12219	VB	denotes	were
T5228	12202-12214	NN	denotes	Supernatants
T5227	12199-12200	-RRB-	denotes	)
T5226	12185-12199	NN	denotes	www.qiagen.com
T5225	12183-12184	-COMMA-	denotes	,
T5224	12177-12183	NN	denotes	Qiagen
T5223	12176-12177	-LRB-	denotes	(
T5222	12168-12175	NN	denotes	reagent
T5221	12155-12167	NN	denotes	transfection
T5220	12145-12154	NN	denotes	Effectene
T5219	12139-12144	VB	denotes	using
T5218	12132-12138	NN	denotes	pMD2.G
T5217	12124-12131	NN	denotes	plasmid
T5216	12115-12123	NN	denotes	envelope
T5215	12111-12114	DT	denotes	the
T5214	12107-12110	CC	denotes	and
T5213	12100-12106	NN	denotes	psPAX2
T5212	12092-12099	NN	denotes	plasmid
T5211	12082-12091	NN	denotes	packaging
T5210	12078-12081	DT	denotes	the
T5209	12073-12077	IN	denotes	with
T5208	12061-12072	NN	denotes	combination
T5207	12058-12060	IN	denotes	in
T5206	12049-12057	NN	denotes	plasmids
T5205	12042-12048	NN	denotes	pLKO.1
T5204	12027-12041	JJ	denotes	shRNA-encoding
T5203	12023-12026	DT	denotes	the
T5202	12018-12022	IN	denotes	with
T5201	12009-12017	NN	denotes	HEK-293T
T5200	12004-12008	NN	denotes	line
T5199	11999-12003	NN	denotes	cell
T5198	11989-11998	NN	denotes	packaging
T5197	11985-11988	DT	denotes	the
T5196	11972-11984	VB	denotes	transfecting
T5195	11969-11971	IN	denotes	by
T5194	11959-11968	VB	denotes	generated
T5193	11954-11958	VB	denotes	were
T5192	11944-11953	NN	denotes	sequences
T5191	11938-11943	NN	denotes	shRNA
T5190	11929-11937	VB	denotes	encoding
T5189	11916-11928	NN	denotes	Lentiviruses
T5188	11907-11914	NN	denotes	plasmid
T5187	11900-11906	NN	denotes	pLKO.1
T5186	11896-11899	DT	denotes	the
T5185	11891-11895	IN	denotes	into
T5184	11884-11890	VB	denotes	cloned
T5183	11879-11883	VB	denotes	were
T5182	11875-11878	CC	denotes	and
T5181	11864-11874	NN	denotes	laboratory
T5180	11860-11863	PRP-DOLLAR-	denotes	our
T5179	11857-11859	IN	denotes	in
T5178	11848-11856	VB	denotes	designed
T5177	11843-11847	VB	denotes	were
T5176	11841-11842	-RRB-	denotes	)
T5175	11831-11841	VB	denotes	underlined
T5174	11828-11830	VB	denotes	is
T5173	11819-11827	NN	denotes	sequence
T5172	11812-11818	NN	denotes	target
T5171	11807-11811	NN	denotes	mRNA
T5170	11801-11806	NN	denotes	TRAF6
T5169	11800-11801	-LRB-	denotes	(
T5168	11738-11799	NN	denotes	5′-AATTCAAAAAGGAGAAACCTGTTGTGATTTATGAGACGAATCACAACAGGTTTCT-3′
T5167	11731-11737	NN	denotes	primer
T5166	11723-11730	JJ	denotes	reverse
T5165	11721-11722	-COMMA-	denotes	,
T5164	11660-11721	NN	denotes	5′-CCGGAGAAACCTGTTGTGATTCGTCTCATAAATCACAACAGGTTTCTCCTTTTTG-3′
T5163	11653-11659	NN	denotes	primer
T5162	11645-11652	JJ	denotes	forward
T5161	11644-11645	-LRB-	denotes	(
T5160	11638-11643	NN	denotes	TRAF6
T5159	11632-11637	JJ	denotes	human
T4698	10522-10524	TO	denotes	to
T4697	10518-10521	CD	denotes	335
T4696	10515-10517	NN	denotes	nt
T4695	10514-10515	-LRB-	denotes	(
T4694	10488-10513	NN	denotes	5′-CAACATGCCTGGAATTCAA-3′
T4693	10486-10487	-COLON-	denotes	:
T4692	10476-10486	NN	denotes	transcript
T4691	10470-10475	NN	denotes	NFAT5
T4690	10466-10469	DT	denotes	the
T4689	10463-10465	TO	denotes	to
T4688	10456-10462	JJ	denotes	unique
T4687	10447-10455	NN	denotes	sequence
T4686	10445-10446	DT	denotes	a
T4685	10438-10444	VB	denotes	target
T4684	10435-10437	TO	denotes	to
T4683	10433-10434	-RRB-	denotes	)
T4682	10419-10433	NN	denotes	www.ambion.com
T4681	10417-10418	-COMMA-	denotes	,
T4680	10413-10417	NNP	denotes	Inc.
T4679	10406-10412	NNP	denotes	Ambion
T4678	10405-10406	-LRB-	denotes	(
T4677	10393-10404	VB	denotes	constructed
T4676	10389-10392	VB	denotes	was
T4675	10383-10388	NN	denotes	siRNA
T4674	10380-10382	DT	denotes	An
T4673	10376-10379	NN	denotes	MDM
T4450	10344-10351	NN	denotes	methods
T4449	10332-10343	NN	denotes	mutagenesis
T4448	10322-10331	JJ	denotes	PCR-based
T4447	10313-10321	JJ	denotes	standard
T4446	10307-10312	VB	denotes	using
T4445	10297-10306	NN	denotes	mutations
T4444	10291-10296	NN	denotes	point
T4443	10279-10290	VB	denotes	introducing
T4442	10276-10278	IN	denotes	by
T4441	10264-10275	VB	denotes	constructed
T4440	10259-10263	VB	denotes	were
T4439	10251-10258	NN	denotes	viruses
T4438	10244-10250	JJ	denotes	mutant
T4437	10232-10243	NN	denotes	HIV-198IN22
T4436	10228-10231	CC	denotes	and
T4435	10211-10227	NN	denotes	HIV-1Lai/Bal-Env
T4434	10198-10209	NN	denotes	HIV-198IN22
T4433	10195-10197	IN	denotes	of
T4432	10187-10194	NN	denotes	isolate
T4431	10179-10186	JJ	denotes	primary
T4430	10177-10178	DT	denotes	a
T4429	10172-10176	IN	denotes	with
T4428	10163-10171	VB	denotes	infected
T4427	10158-10162	VB	denotes	were
T4426	10153-10157	WDT	denotes	that
T4425	10148-10152	NN	denotes	PBMC
T4424	10143-10147	IN	denotes	from
T4423	10133-10142	VB	denotes	extracted
T4422	10129-10132	NN	denotes	DNA
T4421	10123-10128	VB	denotes	using
T4420	10111-10122	VB	denotes	constructed
T4419	10107-10110	VB	denotes	was
T4418	10095-10106	NN	denotes	HIV-198IN22
T4417	10092-10094	IN	denotes	of
T4416	10086-10091	NN	denotes	clone
T4415	10076-10085	JJ	denotes	molecular
T4414	10065-10075	JJ	denotes	infectious
T4413	10061-10064	DT	denotes	The
T4412	10051-10059	NN	denotes	receptor
T4411	10041-10050	JJ	denotes	secondary
T4410	10039-10040	DT	denotes	a
T4409	10036-10038	IN	denotes	as
T4408	10031-10035	NN	denotes	CCR5
T4407	10026-10030	VB	denotes	uses
T4406	10020-10025	NN	denotes	virus
T4405	10011-10019	JJ	denotes	chimeric
T4404	9994-10010	NN	denotes	HIV-1Lai/Bal-Env
T4403	9990-9993	DT	denotes	The
T4402	9987-9988	-RRB-	denotes	)
T4401	9983-9987	NN	denotes	CCR5
T4400	9974-9982	VB	denotes	utilizes
T4399	9969-9973	WDT	denotes	that
T4398	9961-9968	NN	denotes	subtype
T4397	9959-9960	NN	denotes	B
T4396	9958-9959	-LRB-	denotes	(
T4395	9949-9957	JJ	denotes	HIV-1Bal
T4394	9946-9948	IN	denotes	of
T4393	9939-9945	NN	denotes	region
T4392	9925-9938	JJ	denotes	corresponding
T4391	9921-9924	DT	denotes	the
T4390	9916-9920	IN	denotes	with
T4389	9910-9915	NN	denotes	clone
T4388	9900-9909	JJ	denotes	molecular
T4387	9898-9899	-RRB-	denotes	)
T4386	9893-9898	NN	denotes	CXCR4
T4385	9884-9892	VB	denotes	utilizes
T4384	9879-9883	WDT	denotes	that
T4383	9871-9878	NN	denotes	subtype
T4382	9869-9870	NN	denotes	B
T4381	9868-9869	-LRB-	denotes	(
T4380	9859-9867	NN	denotes	HIV-1Lai
T4379	9855-9858	DT	denotes	the
T4378	9852-9854	IN	denotes	of
T4377	9844-9851	CD	denotes	103–717
T4376	9838-9843	NN	denotes	acids
T4375	9832-9837	NN	denotes	amino
T4374	9826-9831	NN	denotes	gp160
T4373	9824-9825	-RRB-	denotes	)
T4372	9821-9824	NN	denotes	env
T4371	9820-9821	-LRB-	denotes	(
T4370	9811-9819	NN	denotes	envelope
T4369	9807-9810	DT	denotes	the
T4368	9797-9806	VB	denotes	replacing
T4367	9794-9796	IN	denotes	by
T4366	9782-9793	VB	denotes	constructed
T4365	9778-9781	VB	denotes	was
T4364	9772-9777	NN	denotes	clone
T4363	9762-9771	JJ	denotes	molecular
T4362	9751-9761	JJ	denotes	infectious
T4361	9734-9750	NN	denotes	HIV-1Lai/Bal-Env
T4360	9730-9733	DT	denotes	The
T4359	9721-9729	NNP	denotes	Program.
T4358	9711-9720	NNP	denotes	Reference
T4357	9707-9710	CC	denotes	and
T4356	9699-9706	NNP	denotes	Reagent
T4355	9694-9698	NNP	denotes	AIDS
T4354	9690-9693	NNP	denotes	NIH
T4353	9686-9689	DT	denotes	the
T4352	9681-9685	IN	denotes	from
T4351	9672-9680	VB	denotes	obtained
T4350	9668-9671	VB	denotes	was
T4349	9659-9667	NN	denotes	HIV-1Lai
T4348	9656-9658	IN	denotes	of
T4347	9650-9655	NN	denotes	clone
T4346	9640-9649	JJ	denotes	molecular
T4345	9629-9639	JJ	denotes	infectious
T4344	9617-9628	JJ	denotes	full-length
T4343	9613-9616	DT	denotes	the
T4342	9604-9612	VB	denotes	encoding
T4341	9596-9603	NN	denotes	plasmid
T4340	9592-9595	DT	denotes	The
T4339	9585-9591	NN	denotes	clones
T4338	9575-9584	JJ	denotes	molecular
T4337	9564-9574	JJ	denotes	infectious
T4336	9558-9563	NN	denotes	HIV-1
T4172	9554-9555	-RRB-	denotes	]
T4171	9552-9554	CD	denotes	31
T4170	9551-9552	-LRB-	denotes	[
T4169	9541-9550	VB	denotes	described
T4168	9530-9540	RB	denotes	previously
T4167	9527-9529	IN	denotes	as
T4166	9517-9526	VB	denotes	performed
T4165	9513-9516	VB	denotes	was
T4164	9500-9512	NN	denotes	footprinting
T4163	9498-9499	CD	denotes	I
T4162	9492-9497	NN	denotes	DNase
T4161	9479-9491	JJ	denotes	Quantitative
T4160	9476-9477	-RRB-	denotes	)
T4159	9457-9476	NN	denotes	www.activemotif.com
T4158	9455-9456	-COMMA-	denotes	,
T4157	9450-9455	NNP	denotes	Motif
T4156	9443-9449	JJ	denotes	Active
T4155	9442-9443	-LRB-	denotes	(
T4154	9432-9441	VB	denotes	purchased
T4153	9427-9431	VB	denotes	were
T4152	9423-9426	NN	denotes	p65
T4151	9419-9422	CC	denotes	and
T4150	9415-9418	NN	denotes	p50
T4149	9403-9414	JJ	denotes	Recombinant
T4148	9400-9401	-RRB-	denotes	)
T4147	9394-9400	NN	denotes	Qiagen
T4146	9393-9394	-LRB-	denotes	(
T4145	9385-9392	NN	denotes	agarose
T4144	9378-9384	NN	denotes	Ni-NTA
T4143	9372-9377	VB	denotes	using
T4142	9361-9371	NN	denotes	conditions
T4141	9354-9360	JJ	denotes	native
T4140	9348-9353	IN	denotes	under
T4139	9339-9347	VB	denotes	purified
T4138	9335-9338	CC	denotes	and
T4137	9333-9334	-RRB-	denotes	)
T4136	9315-9333	NN	denotes	www.stratagene.com
T4135	9313-9314	-COLON-	denotes	;
T4134	9303-9313	NN	denotes	Stratagene
T4133	9302-9303	-LRB-	denotes	(
T4132	9296-9301	NN	denotes	cells
T4131	9294-9295	-RRB-	denotes	)
T4130	9291-9294	NN	denotes	DE3
T4129	9290-9291	-LRB-	denotes	(
T4128	9286-9290	NN	denotes	BL21
T4127	9281-9285	FW	denotes	coli
T4126	9278-9280	FW	denotes	E.
T4125	9275-9277	IN	denotes	in
T4124	9265-9274	VB	denotes	expressed
T4123	9261-9264	VB	denotes	was
T4122	9257-9260	NN	denotes	tag
T4121	9253-9256	PRP-DOLLAR-	denotes	His
T4120	9250-9252	CD	denotes	6×
T4119	9239-9249	JJ	denotes	N-terminal
T4118	9236-9238	DT	denotes	an
T4117	9231-9235	IN	denotes	with
T4116	9229-9230	-RRB-	denotes	)
T4115	9222-9229	CD	denotes	175–471
T4114	9216-9221	NN	denotes	acids
T4113	9210-9215	NN	denotes	amino
T4112	9209-9210	-LRB-	denotes	(
T4111	9203-9208	NN	denotes	NFAT5
T4110	9191-9202	JJ	denotes	Recombinant
T4109	9178-9190	NN	denotes	footprinting
T4108	9176-9177	CD	denotes	I
T4107	9170-9175	NN	denotes	DNase
T4106	9157-9169	JJ	denotes	Quantitative
T4672	10373-10375	IN	denotes	of
T4671	10360-10372	NN	denotes	transfection
T4670	10354-10359	NN	denotes	siRNA
T3793	9128-9129	-LRB-	denotes	(
T3792	9119-9127	NN	denotes	protocol
T3791	9116-9118	POS	denotes	's
T3790	9104-9116	NN	denotes	manufacturer
T3789	9100-9103	DT	denotes	the
T3788	9097-9099	TO	denotes	to
T3787	9087-9096	VB	denotes	according
T3786	9081-9086	NN	denotes	assay
T3785	9072-9080	NN	denotes	reporter
T3784	9056-9071	NN	denotes	dual-luciferase
T3783	9053-9055	IN	denotes	by
T3782	9041-9052	VB	denotes	quantitated
T3781	9037-9040	VB	denotes	was
T3780	9026-9036	NN	denotes	expression
T3779	9021-9025	NN	denotes	gene
T3778	9012-9020	NN	denotes	Reporter
T3777	9005-9010	NN	denotes	hours
T3776	9003-9004	CD	denotes	8
T3775	8999-9002	IN	denotes	for
T3774	8986-8998	JJ	denotes	unstimulated
T3773	8981-8985	VB	denotes	left
T3772	8978-8980	CC	denotes	or
T3771	8971-8977	NN	denotes	lysate
T3770	8963-8970	NN	denotes	CDC1551
T3769	8959-8962	NN	denotes	MTb
T3768	8953-8958	NN	denotes	µg/ml
T3767	8950-8952	CD	denotes	10
T3766	8945-8949	IN	denotes	with
T3765	8934-8944	VB	denotes	stimulated
T3764	8929-8933	VB	denotes	were
T3763	8924-8928	PRP	denotes	they
T3762	8918-8923	WDT	denotes	which
T3761	8912-8917	IN	denotes	after
T3760	8906-8911	NN	denotes	hours
T3759	8903-8905	CD	denotes	16
T3758	8899-8902	IN	denotes	for
T3757	8894-8898	NN	denotes	37°C
T3756	8891-8893	IN	denotes	at
T3755	8881-8890	VB	denotes	incubated
T3754	8876-8880	VB	denotes	were
T3753	8870-8875	NN	denotes	Cells
T3752	8867-8868	-RRB-	denotes	)
T3751	8853-8867	NN	denotes	www.qiagen.com
T3750	8851-8852	-COLON-	denotes	;
T3749	8845-8851	NN	denotes	Qiagen
T3748	8844-8845	-LRB-	denotes	(
T3747	8836-8843	NN	denotes	reagent
T3746	8823-8835	NN	denotes	transfection
T3745	8813-8822	NN	denotes	Effectene
T3744	8807-8812	VB	denotes	using
T3743	8800-8806	NN	denotes	vector
T3742	8792-8799	NN	denotes	control
T3741	8790-8791	-RRB-	denotes	)
T3740	8784-8790	NN	denotes	pRL-TK
T3739	8783-8784	-LRB-	denotes	(
T3738	8772-8782	NN	denotes	luciferase
T3737	8764-8771	NNP	denotes	Renilla
T3736	8758-8763	NN	denotes	µg/ml
T3735	8753-8757	CD	denotes	0.03
T3734	8748-8752	IN	denotes	with
T3733	8736-8747	NN	denotes	combination
T3732	8733-8735	IN	denotes	in
T3731	8724-8732	NN	denotes	plasmids
T3730	8715-8723	NN	denotes	reporter
T3729	8707-8714	VB	denotes	mutated
T3728	8704-8706	CC	denotes	or
T3727	8702-8703	-RRB-	denotes	)
T3726	8700-8702	NN	denotes	WT
T3725	8699-8700	-LRB-	denotes	(
T3724	8689-8698	JJ	denotes	wild-type
T3723	8685-8688	NN	denotes	LTR
T3722	8679-8684	NN	denotes	µg/ml
T3721	8675-8678	CD	denotes	0.3
T3720	8670-8674	IN	denotes	with
T3719	8658-8669	VB	denotes	transfected
T3718	8653-8657	VB	denotes	were
T3717	8651-8652	-RRB-	denotes	)
T3716	8641-8651	NN	denotes	0.8×106/ml
T3715	8640-8641	-LRB-	denotes	(
T3714	8634-8639	NN	denotes	cells
T3713	8628-8633	NN	denotes	THP-1
T3712	8625-8626	-RRB-	denotes	]
T3711	8623-8625	CD	denotes	34
T3710	8622-8623	-LRB-	denotes	[
T3709	8614-8621	NN	denotes	methods
T3708	8602-8613	NN	denotes	mutagenesis
T3707	8592-8601	JJ	denotes	PCR-based
T3706	8583-8591	JJ	denotes	standard
T3705	8580-8582	IN	denotes	by
T3704	8572-8579	VB	denotes	created
T3703	8568-8571	VB	denotes	was
T3702	8560-8567	NN	denotes	plasmid
T3701	8551-8559	NN	denotes	reporter
T3700	8549-8550	-RRB-	denotes	)
T3699	8543-8549	NN	denotes	N5-Mut
T3698	8542-8543	-LRB-	denotes	(
T3697	8530-8541	JJ	denotes	site-mutant
T3696	8522-8529	NN	denotes	binding
T3695	8516-8521	NN	denotes	NFAT5
T3694	8507-8515	NN	denotes	HIV-1Lai
T3693	8503-8506	DT	denotes	The
T3692	8500-8501	-RRB-	denotes	)
T3691	8477-8500	NN	denotes	www.ebi.ac.uk/clustalw/
T3690	8476-8477	-LRB-	denotes	(
T3689	8474-8475	NNP	denotes	W
T3688	8466-8473	NN	denotes	CLUSTAL
T3687	8461-8465	IN	denotes	with
T3686	8452-8460	VB	denotes	analyzed
T3685	8448-8451	CC	denotes	and
T3684	8440-8447	VB	denotes	aligned
T3683	8435-8439	VB	denotes	were
T3682	8425-8434	NN	denotes	Sequences
T3681	8418-8423	NN	denotes	sites
T3680	8411-8417	NN	denotes	enzyme
T3679	8399-8410	NN	denotes	restriction
T3678	8395-8398	CD	denotes	III
T3677	8390-8394	NN	denotes	Hind
T3676	8386-8389	CC	denotes	and
T3675	8384-8385	CD	denotes	I
T3674	8380-8383	NN	denotes	Xho
T3673	8374-8379	VB	denotes	using
T3672	8372-8373	-RRB-	denotes	)
T3671	8357-8372	NN	denotes	www.promega.com
T3670	8355-8356	-COMMA-	denotes	,
T3669	8344-8355	NNP	denotes	BioSciences
T3668	8336-8343	NNP	denotes	Promega
T3667	8335-8336	-LRB-	denotes	(
T3666	8330-8334	NN	denotes	pGL3
T3665	8323-8329	NN	denotes	vector
T3664	8314-8322	NN	denotes	reporter
T3663	8310-8313	DT	denotes	the
T3662	8305-8309	IN	denotes	into
T3661	8303-8304	-RRB-	denotes	)
T3660	8296-8303	NN	denotes	subtype
T3659	8294-8295	NN	denotes	E
T3658	8293-8294	-LRB-	denotes	(
T3657	8283-8292	NN	denotes	HIV-1KR25
T3656	8279-8282	CC	denotes	and
T3655	8277-8278	-COMMA-	denotes	,
T3654	8266-8277	NN	denotes	HIV-192TH51
T3653	8264-8265	-COMMA-	denotes	,
T3652	8253-8264	NN	denotes	HIV-192TH53
T3651	8251-8252	-COMMA-	denotes	,
T3650	8240-8251	NN	denotes	HIV-193TH64
T3649	8238-8239	-COMMA-	denotes	,
T3648	8237-8238	-RRB-	denotes	)
T3647	8230-8237	NN	denotes	subtype
T3646	8228-8229	NN	denotes	C
T3645	8227-8228	-LRB-	denotes	(
T3644	8218-8226	NN	denotes	HIV-1CM9
T3643	8216-8217	-COMMA-	denotes	,
T3642	8205-8216	NN	denotes	HIV-198CH01
T3641	8203-8204	-COMMA-	denotes	,
T3640	8192-8203	NN	denotes	HIV-198IN22
T3639	8190-8191	-COMMA-	denotes	,
T3638	8179-8190	NN	denotes	HIV-198IN17
T3637	8177-8178	-COMMA-	denotes	,
T3636	8176-8177	-RRB-	denotes	)
T3635	8169-8176	NN	denotes	subtype
T3634	8167-8168	NN	denotes	B
T3633	8166-8167	-LRB-	denotes	(
T3632	8157-8165	JJ	denotes	HIV-1Bal
T3631	8155-8156	-COMMA-	denotes	,
T3630	8147-8155	NN	denotes	HIV-1Lai
T3629	8144-8146	IN	denotes	of
T3628	8139-8143	NN	denotes	site
T3627	8128-8138	NN	denotes	initiation
T3626	8112-8127	JJ	denotes	transcriptional
T3625	8108-8111	DT	denotes	the
T3624	8105-8107	TO	denotes	to
T3623	8096-8104	JJ	denotes	relative
T3622	8092-8095	CD	denotes	+64
T3621	8089-8091	TO	denotes	to
T3620	8084-8088	CD	denotes	−208
T3619	8072-8083	NN	denotes	nucleotides
T3618	8062-8071	VB	denotes	inserting
T3617	8059-8061	IN	denotes	by
T3616	8047-8058	VB	denotes	constructed
T3615	8042-8046	VB	denotes	were
T3614	8033-8041	NN	denotes	plasmids
T3613	8024-8032	NN	denotes	reporter
T3612	8020-8023	NN	denotes	LTR
T3611	8014-8019	NN	denotes	assay
T3610	8005-8013	NN	denotes	reporter
T3609	8001-8004	CC	denotes	and
T3608	7988-8000	NN	denotes	construction
T3607	7980-7987	NN	denotes	plasmid
T3606	7976-7979	NN	denotes	LTR
T3797	9153-9154	-RRB-	denotes	)
T3796	9138-9153	NN	denotes	www.promega.com
T3795	9136-9137	-COLON-	denotes	;
T3794	9129-9136	NNP	denotes	Promega
T3164	7663-7666	NN	denotes	FCS
T3163	7661-7662	NN	denotes	%
T3162	7659-7661	CD	denotes	10
T3161	7654-7658	IN	denotes	with
T3160	7641-7653	VB	denotes	supplemented
T3159	7639-7640	-RRB-	denotes	)
T3062	7086-7090	NN	denotes	cell
T3061	7082-7085	DT	denotes	The
T3060	7079-7080	-RRB-	denotes	)
T3059	7077-7079	NN	denotes	FL
T3058	7075-7076	-COMMA-	denotes	,
T3057	7070-7075	NNP	denotes	Raton
T3056	7065-7069	NNP	denotes	Boca
T3055	7063-7064	-COMMA-	denotes	,
T3054	7059-7063	NNP	denotes	Nabi
T3053	7058-7059	-LRB-	denotes	(
T3052	7052-7057	NN	denotes	serum
T3051	7049-7051	NN	denotes	AB
T3050	7043-7048	JJ	denotes	human
T3049	7026-7042	JJ	denotes	heat-inactivated
T3048	7024-7025	NN	denotes	%
T3047	7023-7024	CD	denotes	5
T3046	7019-7022	CC	denotes	and
T3045	7017-7018	-RRB-	denotes	)
T3044	6999-7017	NN	denotes	www.rndsystems.com
T3043	6997-6998	-COMMA-	denotes	,
T3042	6996-6997	NNP	denotes	D
T3041	6995-6996	CC	denotes	&
T3040	6994-6995	NN	denotes	R
T3039	6993-6994	-LRB-	denotes	(
T3038	6988-6992	NN	denotes	MCSF
T3037	6982-6987	JJ	denotes	human
T3036	6970-6981	JJ	denotes	recombinant
T3035	6964-6969	NN	denotes	ng/ml
T3034	6961-6963	CD	denotes	15
T3033	6956-6960	IN	denotes	with
T3032	6943-6955	VB	denotes	supplemented
T3031	6941-6942	-RRB-	denotes	)
T3030	6923-6941	NN	denotes	www.invitrogen.com
T3029	6921-6922	-COMMA-	denotes	,
T3028	6916-6921	NNP	denotes	Gibco
T3027	6915-6916	-LRB-	denotes	(
T3026	6908-6914	NN	denotes	medium
T3025	6893-6907	JJ	denotes	Macrophage-SFM
T3024	6890-6892	IN	denotes	in
T3023	6883-6889	NN	denotes	plates
T3022	6876-6882	JJ	denotes	6-well
T3021	6873-6875	IN	denotes	in
T3020	6868-6872	RB	denotes	well
T3019	6864-6867	IN	denotes	per
T3018	6858-6863	NN	denotes	cells
T3017	6852-6857	CD	denotes	1×106
T3016	6849-6851	IN	denotes	at
T3015	6840-6848	VB	denotes	cultured
T3014	6835-6839	VB	denotes	were
T3013	6831-6834	CC	denotes	and
T3012	6829-6830	-COMMA-	denotes	,
T3011	6817-6829	NN	denotes	manufacturer
T3010	6813-6816	DT	denotes	the
T3009	6810-6812	IN	denotes	by
T3008	6800-6809	VB	denotes	described
T3007	6797-6799	IN	denotes	as
T3006	6795-6796	-RRB-	denotes	)
T3005	6773-6795	NN	denotes	www.miltenyibiotec.com
T3004	6772-6773	-LRB-	denotes	(
T3003	6765-6771	NNP	denotes	Biotec
T3002	6756-6764	NNP	denotes	Miltenyi
T3001	6751-6755	IN	denotes	from
T3000	6740-6750	NN	denotes	microbeads
T2999	6735-6739	NN	denotes	CD14
T2998	6730-6734	IN	denotes	with
T2997	6720-6729	NN	denotes	selection
T2996	6711-6719	JJ	denotes	positive
T2995	6708-6710	IN	denotes	by
T2994	6695-6707	NN	denotes	preparations
T2993	6690-6694	NN	denotes	PBMC
T2992	6685-6689	IN	denotes	from
T2991	6676-6684	VB	denotes	isolated
T2990	6671-6675	VB	denotes	were
T2989	6661-6670	NN	denotes	monocytes
T2988	6655-6660	JJ	denotes	Human
T2987	6652-6653	-RRB-	denotes	)
T2986	6638-6652	NN	denotes	www.gembio.com
T2985	6636-6637	-COMMA-	denotes	,
T2984	6624-6636	NNP	denotes	Bio-Products
T2983	6617-6623	NNP	denotes	Gemini
T2982	6616-6617	-LRB-	denotes	(
T2981	6614-6615	-RRB-	denotes	)
T2980	6611-6614	NN	denotes	FCS
T2979	6610-6611	-LRB-	denotes	(
T2978	6604-6609	NN	denotes	serum
T2977	6599-6603	NN	denotes	calf
T2976	6593-6598	JJ	denotes	fetal
T2975	6576-6592	JJ	denotes	heat-inactivated
T2974	6574-6575	NN	denotes	%
T2973	6572-6574	CD	denotes	10
T2972	6567-6571	IN	denotes	with
T2971	6554-6566	VB	denotes	supplemented
T2970	6552-6553	-RRB-	denotes	)
T2969	6550-6552	NNP	denotes	MD
T2968	6548-6549	-COMMA-	denotes	,
T2967	6536-6548	NNP	denotes	Walkersville
T2966	6534-6535	-COMMA-	denotes	,
T2965	6530-6534	NNP	denotes	Inc.
T2964	6528-6529	-COMMA-	denotes	,
T2963	6516-6528	NNP	denotes	BioWhittaker
T2962	6515-6516	-LRB-	denotes	(
T2961	6503-6514	NN	denotes	L-glutamine
T2960	6500-6502	NN	denotes	mM
T2959	6498-6499	CD	denotes	2
T2958	6493-6497	IN	denotes	with
T2957	6486-6492	NN	denotes	medium
T2956	6481-6485	CD	denotes	1640
T2955	6476-6480	NN	denotes	RPMI
T2954	6473-6475	IN	denotes	in
T2953	6464-6472	VB	denotes	cultured
T2952	6459-6463	VB	denotes	were
T2951	6455-6458	CC	denotes	and
T2950	6440-6454	NN	denotes	centrifugation
T2949	6431-6439	NN	denotes	gradient
T2948	6423-6430	NN	denotes	density
T2947	6421-6422	-RRB-	denotes	)
T2946	6419-6421	NNP	denotes	NJ
T2945	6417-6418	-COMMA-	denotes	,
T2944	6410-6417	NNP	denotes	Peapack
T2943	6408-6409	-COMMA-	denotes	,
T2942	6397-6408	NNP	denotes	Corporation
T2941	6387-6396	NNP	denotes	Pharmacia
T2940	6386-6387	-LRB-	denotes	(
T2939	6371-6385	JJ	denotes	Ficoll-Hypaque
T2938	6368-6370	IN	denotes	by
T2937	6359-6367	VB	denotes	isolated
T2936	6354-6358	VB	denotes	were
T2935	6347-6353	NN	denotes	donors
T2934	6334-6346	JJ	denotes	unidentified
T2933	6327-6333	JJ	denotes	normal
T2932	6322-6326	IN	denotes	from
T2931	6317-6321	NN	denotes	PBMC
T2930	6309-6316	NN	denotes	culture
T2929	6304-6308	NN	denotes	Cell
T3517	7972-7973	-RRB-	denotes	]
T3516	7970-7972	CD	denotes	33
T3515	7969-7970	-LRB-	denotes	[
T3514	7962-7968	IN	denotes	before
T3513	7952-7961	VB	denotes	described
T3512	7949-7951	IN	denotes	as
T3511	7938-7948	NN	denotes	laboratory
T3510	7934-7937	PRP-DOLLAR-	denotes	our
T3509	7931-7933	IN	denotes	in
T3508	7922-7930	VB	denotes	isolated
T3507	7918-7921	VB	denotes	was
T3506	7908-7917	NN	denotes	HIV-1KR25
T3505	7899-7907	NNP	denotes	Kingdom.
T3504	7892-7898	NNP	denotes	United
T3503	7890-7891	-COMMA-	denotes	,
T3502	7889-7890	-RRB-	denotes	)
T3501	7884-7889	NN	denotes	NIBSC
T3500	7883-7884	-LRB-	denotes	(
T3499	7875-7882	NNP	denotes	Control
T3498	7871-7874	CC	denotes	and
T3497	7862-7870	NNP	denotes	Standard
T3496	7851-7861	NNP	denotes	Biological
T3495	7847-7850	IN	denotes	for
T3494	7837-7846	NNP	denotes	Institute
T3493	7828-7836	NNP	denotes	National
T3492	7826-7827	-COMMA-	denotes	,
T3491	7818-7826	NNP	denotes	Reagents
T3490	7813-7817	NNP	denotes	AIDS
T3489	7809-7812	IN	denotes	for
T3488	7800-7808	NNP	denotes	Facility
T3487	7788-7799	NNP	denotes	Centralized
T3486	7784-7787	DT	denotes	The
T3485	7779-7783	IN	denotes	from
T3484	7770-7778	VB	denotes	obtained
T3483	7765-7769	VB	denotes	were
T3482	7753-7764	NN	denotes	HIV-198IN22
T3481	7749-7752	CC	denotes	and
T3480	7747-7748	-COMMA-	denotes	,
T3479	7736-7747	NN	denotes	HIV-198CH01
T3478	7734-7735	-COMMA-	denotes	,
T3477	7723-7734	NN	denotes	HIV-192TH53
T3476	7721-7722	-COMMA-	denotes	,
T3475	7710-7721	NN	denotes	HIV-192TH51
T3474	7708-7709	-COMMA-	denotes	,
T3473	7697-7708	NN	denotes	HIV-193TH64
T3472	7695-7696	-COMMA-	denotes	,
T3471	7687-7695	NN	denotes	HIV-1Lai
T3470	7685-7686	-COMMA-	denotes	,
T3469	7677-7685	JJ	denotes	HIV-1Bal
T3468	7669-7676	NN	denotes	Viruses
T2847	6295-6301	NNP	denotes	Boston
T3158	7621-7639	NN	denotes	www.invitrogen.com
T3157	7619-7620	-COMMA-	denotes	,
T3156	7614-7619	NNP	denotes	Gibco
T3155	7613-7614	-LRB-	denotes	(
T3154	7611-7612	-RRB-	denotes	)
T3153	7607-7611	NN	denotes	DMEM
T3152	7606-7607	-LRB-	denotes	(
T3151	7599-7605	NN	denotes	medium
T3150	7596-7598	POS	denotes	's
T3149	7591-7596	NNP	denotes	Eagle
T3148	7582-7590	NNP	denotes	Modified
T3147	7579-7581	POS	denotes	's
T3146	7571-7579	NNP	denotes	Dulbecco
T3145	7568-7570	IN	denotes	in
T3144	7557-7567	VB	denotes	maintained
T3143	7552-7556	VB	denotes	were
T3142	7548-7551	CC	denotes	and
T3141	7546-7547	-RRB-	denotes	)
T3140	7534-7546	NN	denotes	www.atcc.org
T3139	7533-7534	-LRB-	denotes	(
T3138	7528-7532	NN	denotes	ATCC
T3137	7523-7527	IN	denotes	from
T3136	7514-7522	VB	denotes	obtained
T3135	7509-7513	VB	denotes	were
T3134	7503-7508	NN	denotes	cells
T3133	7498-7502	NN	denotes	293T
T3132	7495-7496	-RRB-	denotes	)
T3131	7479-7495	NN	denotes	www.lonzabio.com
T3130	7477-7478	-COMMA-	denotes	,
T3129	7465-7477	NNP	denotes	BioWhittaker
T3128	7464-7465	-LRB-	denotes	(
T3127	7460-7463	NN	denotes	FCS
T3126	7458-7459	NN	denotes	%
T3125	7456-7458	CD	denotes	10
T3124	7451-7455	IN	denotes	with
T3123	7438-7450	VB	denotes	supplemented
T3122	7431-7437	NN	denotes	medium
T3121	7426-7430	CD	denotes	1640
T3120	7421-7425	NN	denotes	RPMI
T3119	7418-7420	IN	denotes	in
T3118	7409-7417	VB	denotes	cultured
T3117	7405-7408	CC	denotes	and
T3116	7403-7404	-RRB-	denotes	)
T3115	7391-7403	NN	denotes	www.atcc.org
T3114	7390-7391	-LRB-	denotes	(
T3113	7385-7389	NN	denotes	ATCC
T3112	7380-7384	IN	denotes	from
T3111	7371-7379	VB	denotes	obtained
T3110	7366-7370	VB	denotes	were
T3109	7360-7365	NN	denotes	cells
T3108	7354-7359	NN	denotes	THP-1
T3107	7343-7352	NN	denotes	cytometry
T3106	7338-7342	NN	denotes	flow
T3105	7335-7337	IN	denotes	by
T3104	7326-7334	VB	denotes	verified
T3103	7323-7325	IN	denotes	as
T3102	7311-7322	NN	denotes	macrophages
T3101	7305-7310	JJ	denotes	CD14+
T3100	7300-7304	VB	denotes	were
T3099	7290-7299	NN	denotes	technique
T3098	7285-7289	DT	denotes	this
T3097	7280-7284	IN	denotes	with
T3096	7271-7279	VB	denotes	obtained
T3095	7265-7270	NN	denotes	cells
T3094	7256-7264	JJ	denotes	adherent
T3093	7252-7255	DT	denotes	the
T3092	7249-7251	IN	denotes	of
T3091	7247-7248	NN	denotes	%
T3090	7245-7247	CD	denotes	95
T3089	7240-7244	IN	denotes	than
T3088	7235-7239	JJ	denotes	More
T3087	7221-7233	NN	denotes	manipulation
T3086	7214-7220	IN	denotes	before
T3085	7209-7213	NN	denotes	MCSF
T3084	7201-7208	VB	denotes	lacking
T3083	7194-7200	NN	denotes	medium
T3082	7188-7193	JJ	denotes	fresh
T3081	7183-7187	IN	denotes	with
T3080	7174-7182	VB	denotes	replaced
T3079	7170-7173	VB	denotes	was
T3078	7158-7169	NN	denotes	supernatant
T3077	7152-7157	WDT	denotes	which
T3076	7146-7151	IN	denotes	after
T3075	7144-7145	-COMMA-	denotes	,
T3074	7140-7144	NN	denotes	days
T3073	7138-7139	CD	denotes	5
T3072	7134-7137	IN	denotes	for
T3071	7130-7133	NN	denotes	CO2
T3070	7128-7129	NN	denotes	%
T3069	7127-7128	CD	denotes	5
T3068	7123-7126	CC	denotes	and
T3067	7118-7122	NN	denotes	37°C
T3066	7115-7117	IN	denotes	at
T3065	7105-7114	VB	denotes	incubated
T3064	7100-7104	VB	denotes	were
T3063	7091-7099	NN	denotes	cultures
T2814	6106-6112	NN	denotes	Ethics
T1771	6069-6080	NN	denotes	replication
T1770	6063-6068	NN	denotes	HIV-1
T1769	6060-6062	IN	denotes	of
T1768	6048-6059	NN	denotes	enhancement
T1767	6036-6047	JJ	denotes	MTb-induced
T1766	6033-6035	IN	denotes	of
T1765	6023-6032	NN	denotes	modulator
T1764	6014-6022	JJ	denotes	critical
T1763	6012-6013	DT	denotes	a
T1762	6009-6011	VB	denotes	is
T1761	6003-6008	NN	denotes	NFAT5
T1760	5998-6002	IN	denotes	that
T1759	5986-5997	VB	denotes	demonstrate
T1758	5982-5985	CC	denotes	and
T1757	5980-5981	-COMMA-	denotes	,
T1756	5972-5980	NN	denotes	response
T1755	5965-5971	JJ	denotes	immune
T1754	5958-5964	JJ	denotes	innate
T1753	5945-5957	JJ	denotes	PRR-mediated
T1752	5941-5944	DT	denotes	the
T1751	5938-5940	TO	denotes	to
T1750	5927-5937	NN	denotes	expression
T1749	5921-5926	NN	denotes	NFAT5
T1748	5918-5920	IN	denotes	of
T1747	5910-5917	NN	denotes	stimuli
T1746	5904-5909	JJ	denotes	known
T1745	5900-5903	DT	denotes	the
T1744	5893-5899	VB	denotes	expand
T1743	5885-5892	NN	denotes	results
T1742	5879-5884	DT	denotes	these
T1741	5877-5878	-COMMA-	denotes	,
T1740	5873-5877	RB	denotes	Thus
T1739	5865-5871	NN	denotes	manner
T1738	5849-5864	JJ	denotes	MyD88-dependent
T1737	5847-5848	DT	denotes	a
T1736	5844-5846	IN	denotes	in
T1735	5834-5843	NN	denotes	monocytes
T1734	5828-5833	JJ	denotes	human
T1733	5825-5827	IN	denotes	in
T1732	5814-5824	NN	denotes	expression
T1731	5809-5813	NN	denotes	gene
T1730	5803-5808	NN	denotes	NFAT5
T1729	5793-5802	VB	denotes	increases
T1728	5783-5792	NN	denotes	infection
T1727	5779-5782	NN	denotes	MTb
T1726	5774-5778	IN	denotes	that
T1725	5762-5773	VB	denotes	demonstrate
T1724	5759-5761	PRP	denotes	we
T1723	5757-5758	-COMMA-	denotes	,
T1722	5749-5757	RB	denotes	Moreover
T1721	5743-5747	NN	denotes	PBMC
T1720	5739-5742	CC	denotes	and
T1719	5735-5738	NN	denotes	MDM
T1718	5729-5734	JJ	denotes	human
T1717	5726-5728	IN	denotes	in
T1716	5714-5725	NN	denotes	replication
T1715	5708-5713	NN	denotes	HIV-1
T1714	5705-5707	IN	denotes	of
T1713	5693-5704	NN	denotes	stimulation
T1712	5681-5692	JJ	denotes	MTb-induced
T1711	5671-5680	JJ	denotes	efficient
T1710	5667-5670	IN	denotes	for
T1709	5656-5666	NN	denotes	importance
T1708	5648-5655	JJ	denotes	crucial
T1707	5645-5647	IN	denotes	of
T1706	5641-5644	VB	denotes	are
T1705	5636-5640	NN	denotes	site
T1704	5628-5635	NN	denotes	binding
T1703	5620-5627	JJ	denotes	cognate
T1702	5616-5619	PRP-DOLLAR-	denotes	its
T1701	5612-5615	CC	denotes	and
T1700	5606-5611	NN	denotes	NFAT5
T1699	5601-5605	IN	denotes	that
T1698	5596-5600	VB	denotes	show
T1697	5593-5595	PRP	denotes	we
T1696	5591-5592	-COMMA-	denotes	,
T1695	5587-5591	RB	denotes	Here
T1694	5584-5585	-RRB-	denotes	)
T1693	5580-5584	NN	denotes	PBMC
T1692	5579-5580	-LRB-	denotes	(
T1691	5573-5578	NN	denotes	cells
T1690	5561-5572	JJ	denotes	mononuclear
T1689	5555-5560	NN	denotes	blood
T1688	5544-5554	JJ	denotes	peripheral
T1687	5540-5543	CC	denotes	and
T1686	5530-5539	NN	denotes	monocytes
T1685	5527-5529	IN	denotes	in
T1684	5519-5526	NN	denotes	pathway
T1683	5506-5518	JJ	denotes	TLR-mediated
T1682	5504-5505	DT	denotes	a
T1681	5500-5503	IN	denotes	via
T1680	5488-5499	NN	denotes	replication
T1679	5482-5487	NN	denotes	HIV-1
T1678	5479-5481	IN	denotes	of
T1677	5468-5478	NN	denotes	activation
T1676	5456-5467	JJ	denotes	MTb-induced
T1675	5453-5455	IN	denotes	in
T1674	5444-5452	VB	denotes	involved
T1673	5441-5443	VB	denotes	be
T1672	5436-5440	RB	denotes	also
T1671	5432-5435	MD	denotes	may
T1670	5426-5431	NN	denotes	NFAT5
T1669	5421-5425	IN	denotes	that
T1668	5410-5420	VB	denotes	speculated
T1667	5407-5409	PRP	denotes	we
T1666	5405-5406	-COMMA-	denotes	,
T1665	5404-5405	-RRB-	denotes	]
T1664	5402-5404	CD	denotes	32
T1663	5401-5402	-LRB-	denotes	[
T1662	5399-5400	-COMMA-	denotes	,
T1661	5390-5399	NN	denotes	signaling
T1660	5384-5389	NN	denotes	MyD88
T1659	5381-5383	IN	denotes	of
T1658	5370-5380	JJ	denotes	downstream
T1657	5367-5369	VB	denotes	is
T1656	5361-5366	WDT	denotes	which
T1655	5359-5360	-COMMA-	denotes	,
T1654	5356-5359	NN	denotes	p38
T1653	5349-5355	NN	denotes	kinase
T1652	5345-5348	NN	denotes	MAP
T1651	5341-5344	DT	denotes	the
T1650	5338-5340	IN	denotes	by
T1649	5328-5337	VB	denotes	activated
T2841	6264-6268	NNP	denotes	Bank
T2840	6258-6263	NNP	denotes	Blood
T2839	6254-6257	DT	denotes	the
T2838	6249-6253	IN	denotes	from
T2837	6240-6248	VB	denotes	obtained
T2836	6237-6239	PRP	denotes	we
T2835	6231-6236	WDT	denotes	which
T2834	6229-6230	-COMMA-	denotes	,
T2833	6228-6229	-RRB-	denotes	)
T2832	6224-6228	NN	denotes	PBMC
T2831	6222-6223	-COMMA-	denotes	,
T2830	6217-6222	NN	denotes	cells
T2829	6205-6216	JJ	denotes	mononuclear
T2828	6199-6204	NN	denotes	blood
T2827	6188-6198	JJ	denotes	peripheral
T2826	6187-6188	-LRB-	denotes	(
T2825	6181-6186	NN	denotes	cells
T2824	6175-6180	NN	denotes	blood
T2823	6165-6174	VB	denotes	discarded
T2822	6159-6164	JJ	denotes	human
T2821	6146-6158	JJ	denotes	unidentified
T2820	6141-6145	VB	denotes	used
T2819	6138-6140	PRP	denotes	we
T2818	6130-6137	NN	denotes	studies
T2817	6126-6129	PRP-DOLLAR-	denotes	our
T2816	6123-6125	IN	denotes	In
T2815	6113-6122	NN	denotes	statement
T1648	5310-5327	RB	denotes	transcriptionally
T1647	5307-5309	VB	denotes	be
T1646	5304-5306	TO	denotes	to
T1645	5298-5303	VB	denotes	shown
T1644	5293-5297	VB	denotes	been
T1643	5282-5292	RB	denotes	previously
T1642	5278-5281	VB	denotes	has
T1641	5272-5277	NN	denotes	NFAT5
T1640	5267-5271	IN	denotes	that
T1639	5261-5266	VB	denotes	Given
T1638	5258-5259	-RRB-	denotes	]
T1637	5256-5258	CD	denotes	31
T1636	5255-5256	-LRB-	denotes	[
T1635	5253-5254	-RRB-	denotes	)
T1634	5250-5253	NN	denotes	MDM
T1633	5249-5250	-LRB-	denotes	(
T1632	5237-5248	NN	denotes	macrophages
T1631	5220-5236	JJ	denotes	monocyte-derived
T1630	5212-5219	JJ	denotes	primary
T1629	5206-5211	JJ	denotes	human
T1628	5203-5205	IN	denotes	in
T1627	5194-5202	NN	denotes	isolates
T1626	5192-5193	NN	denotes	E
T1625	5188-5191	CC	denotes	and
T1624	5186-5187	-COMMA-	denotes	,
T1623	5185-5186	NN	denotes	C
T1541	4758-4759	-LRB-	denotes	[
T1540	4757-4758	CD	denotes	–
T1539	4756-4757	-RRB-	denotes	]
T1538	4754-4756	CD	denotes	28
T1537	4753-4754	-LRB-	denotes	[
T1536	4751-4752	-COMMA-	denotes	,
T1535	4750-4751	-RRB-	denotes	]
T1534	4749-4750	CD	denotes	1
T1533	4748-4749	-LRB-	denotes	[
T1532	4735-4747	RB	denotes	respectively
T1531	4733-4734	-COMMA-	denotes	,
T1530	4728-4733	NN	denotes	sites
T1529	4720-4727	NN	denotes	binding
T1528	4714-4719	NN	denotes	NF-κB
T1527	4710-4713	CD	denotes	one
T1526	4706-4709	CC	denotes	and
T1525	4700-4705	CD	denotes	three
T1524	4695-4699	VB	denotes	have
T1523	4693-4694	-COMMA-	denotes	,
T1522	4689-4693	NNP	denotes	Asia
T1521	4679-4688	JJ	denotes	southeast
T1520	4675-4678	CC	denotes	and
T1519	4668-4674	NNP	denotes	Africa
T1518	4656-4667	JJ	denotes	sub-Saharan
T1517	4644-4655	JJ	denotes	TB-burdened
T1516	4641-4643	IN	denotes	in
T1515	4622-4640	RB	denotes	disproportionately
T1514	4615-4621	VB	denotes	spread
T1513	4610-4614	VB	denotes	have
T1512	4604-4609	WDT	denotes	which
T1511	4602-4603	-COMMA-	denotes	,
T1510	4601-4602	NN	denotes	E
T1509	4597-4600	CC	denotes	and
T1508	4595-4596	NN	denotes	C
T1507	4586-4594	NN	denotes	subtypes
T1506	4580-4585	NN	denotes	HIV-1
T1505	4578-4579	-COMMA-	denotes	,
T1504	4570-4578	NN	denotes	contrast
T1503	4567-4569	IN	denotes	By
T1502	4562-4565	NN	denotes	LTR
T1501	4558-4561	PRP-DOLLAR-	denotes	its
T1500	4555-4557	IN	denotes	in
T1499	4548-4554	NN	denotes	motifs
T1498	4542-4547	NN	denotes	NF-κB
T1497	4535-4541	JJ	denotes	tandem
T1496	4531-4534	CD	denotes	two
T1495	4527-4530	VB	denotes	has
T1494	4525-4526	-COMMA-	denotes	,
T1493	4516-4525	NNP	denotes	Australia
T1492	4512-4515	CC	denotes	and
T1491	4510-4511	-COMMA-	denotes	,
T1490	4505-4510	NNP	denotes	Japan
T1489	4503-4504	-COMMA-	denotes	,
T1488	4497-4503	NNP	denotes	Europe
T1487	4495-4496	-COMMA-	denotes	,
T1486	4487-4495	NNP	denotes	Americas
T1485	4483-4486	DT	denotes	the
T1484	4480-4482	IN	denotes	in
T1483	4470-4479	NN	denotes	infection
T1482	4467-4469	IN	denotes	of
T1481	4461-4466	NN	denotes	cause
T1480	4453-4460	JJ	denotes	primary
T1479	4449-4452	DT	denotes	the
T1478	4445-4448	CC	denotes	and
T1477	4437-4444	NN	denotes	subtype
T1476	4431-4436	JJ	denotes	viral
T1475	4417-4430	VB	denotes	characterized
T1474	4410-4416	RB	denotes	highly
T1473	4405-4409	RB	denotes	most
T1472	4401-4404	DT	denotes	the
T1471	4399-4400	-COMMA-	denotes	,
T1470	4398-4399	NN	denotes	B
T1469	4390-4397	NN	denotes	subtype
T1468	4384-4389	NN	denotes	HIV-1
T1467	4382-4383	-COMMA-	denotes	,
T1466	4375-4382	NN	denotes	example
T1465	4371-4374	IN	denotes	For
T1464	4364-4369	NN	denotes	sites
T1463	4356-4363	NN	denotes	binding
T1462	4346-4355	NN	denotes	activator
T1461	4343-4345	IN	denotes	of
T1460	4330-4342	NN	denotes	organization
T1459	4326-4329	CC	denotes	and
T1458	4319-4325	NN	denotes	number
T1457	4315-4318	DT	denotes	the
T1456	4312-4314	TO	denotes	to
T1455	4304-4311	NN	denotes	respect
T1454	4299-4303	IN	denotes	with
T1453	4292-4298	JJ	denotes	unique
T1452	4289-4291	VB	denotes	is
T1451	4281-4288	NN	denotes	subtype
T1450	4276-4280	DT	denotes	each
T1449	4273-4275	IN	denotes	of
T1448	4269-4272	NN	denotes	LTR
T1447	4265-4268	DT	denotes	the
T1446	4261-4264	CC	denotes	and
T1445	4259-4260	-COMMA-	denotes	,
T1444	4251-4259	NN	denotes	subtypes
T1443	4243-4250	JJ	denotes	several
T1442	4233-4242	VB	denotes	comprises
T1441	4227-4232	NN	denotes	HIV-1
T1440	4225-4226	-COMMA-	denotes	,
T1439	4218-4225	RB	denotes	Notably
T1438	4215-4216	-RRB-	denotes	)
T1437	4214-4215	-RRB-	denotes	]
T1436	4212-4214	CD	denotes	10
T1435	4211-4212	-LRB-	denotes	[
T1434	4208-4210	IN	denotes	in
T1433	4199-4207	VB	denotes	reviewed
T1432	4197-4198	-COMMA-	denotes	,
T1431	4196-4197	-RRB-	denotes	]
T1430	4194-4196	CD	denotes	27
T1429	4193-4194	-LRB-	denotes	[
T1428	4192-4193	CD	denotes	–
T1427	4191-4192	-RRB-	denotes	]
T1426	4189-4191	CD	denotes	25
T1425	4188-4189	-LRB-	denotes	[
T1424	4186-4187	-COMMA-	denotes	,
T1423	4185-4186	-RRB-	denotes	]
T1422	4183-4185	CD	denotes	22
T1421	4182-4183	-LRB-	denotes	[
T1420	4181-4182	-LRB-	denotes	(
T1419	4167-4180	NN	denotes	transcription
T1418	4163-4166	PRP-DOLLAR-	denotes	its
T1417	4157-4162	VB	denotes	drive
T1416	4154-4156	TO	denotes	to
T1415	4150-4153	CC	denotes	and
T1414	4146-4149	NN	denotes	LTR
T1413	4140-4145	NN	denotes	HIV-1
T1412	4136-4139	DT	denotes	the
T1411	4131-4135	IN	denotes	with
T1410	4121-4130	VB	denotes	associate
T1409	4118-4120	TO	denotes	to
T1408	4112-4117	VB	denotes	shown
T1407	4107-4111	VB	denotes	been
T1406	4102-4106	VB	denotes	have
T1405	4096-4101	WDT	denotes	which
T1404	4094-4095	-COMMA-	denotes	,
T1403	4086-4094	NN	denotes	families
T1402	4081-4085	NN	denotes	AP-1
T1401	4077-4080	CC	denotes	and
T1400	4071-4076	NN	denotes	NF-κB
T1399	4067-4070	DT	denotes	the
T1398	4057-4066	VB	denotes	including
T1397	4055-4056	-COMMA-	denotes	,
T1396	4047-4055	NN	denotes	families
T1395	4040-4046	NN	denotes	factor
T1394	4026-4039	NN	denotes	transcription
T1393	4017-4025	JJ	denotes	specific
T1392	4014-4016	IN	denotes	of
T1391	4003-4013	NN	denotes	activation
T1390	3996-4002	VB	denotes	induce
T1389	3985-3995	RB	denotes	ultimately
T1388	3983-3984	-COMMA-	denotes	,
T1387	3979-3983	NN	denotes	turn
T1386	3976-3978	IN	denotes	in
T1385	3974-3975	-COMMA-	denotes	,
T1384	3970-3974	WDT	denotes	that
T1383	3962-3969	NN	denotes	kinases
T1382	3960-3961	-RRB-	denotes	)
T1381	3957-3960	NN	denotes	MAP
T1380	3956-3957	-LRB-	denotes	(
T1379	3948-3955	NN	denotes	protein
T1378	3930-3947	JJ	denotes	mitogen-activated
T1377	3926-3929	CC	denotes	and
T1376	3924-3925	-RRB-	denotes	)
T1375	3921-3924	NN	denotes	IKK
T1374	3920-3921	-LRB-	denotes	(
T1373	3913-3919	NN	denotes	kinase
T1372	3909-3912	NN	denotes	IκB
T1371	3899-3908	VB	denotes	activates
T1370	3893-3898	NN	denotes	TRAF6
T1369	3890-3891	-RRB-	denotes	)
T1368	3889-3890	-RRB-	denotes	]
T1367	3887-3889	CD	denotes	24
T1366	3886-3887	-LRB-	denotes	[
T1365	3884-3885	-COMMA-	denotes	,
T1364	3883-3884	-RRB-	denotes	]
T1363	3881-3883	CD	denotes	10
T1362	3880-3881	-LRB-	denotes	[
T1361	3877-3879	IN	denotes	in
T1360	3868-3876	VB	denotes	reviewed
T1359	3866-3867	-COMMA-	denotes	,
T1358	3865-3866	-RRB-	denotes	]
T1357	3863-3865	CD	denotes	23
T1356	3862-3863	-LRB-	denotes	[
T1355	3861-3862	NN	denotes	–
T1354	3860-3861	-RRB-	denotes	]
T1353	3858-3860	CD	denotes	21
T1352	3857-3858	-LRB-	denotes	[
T1351	3856-3857	-LRB-	denotes	(
T1350	3850-3855	NN	denotes	TRAF6
T1349	3843-3849	NN	denotes	ligase
T1348	3833-3842	NN	denotes	ubiquitin
T1347	3829-3832	DT	denotes	the
T1346	3825-3828	CC	denotes	and
T1345	3823-3824	-COMMA-	denotes	,
T1344	3818-3823	NN	denotes	IRAK4
T1343	3814-3817	CC	denotes	and
T1342	3808-3813	NN	denotes	IRAK1
T1341	3798-3807	VB	denotes	including
T1340	3796-3797	-COMMA-	denotes	,
T1339	3789-3796	NN	denotes	kinases
T1338	3781-3788	JJ	denotes	several
T1337	3778-3780	IN	denotes	of
T1336	3766-3777	NN	denotes	recruitment
T1335	3755-3765	JJ	denotes	subsequent
T1334	3751-3754	DT	denotes	the
T1333	3747-3750	CC	denotes	and
T1332	3741-3746	NN	denotes	MyD88
T1331	3733-3740	NN	denotes	protein
T1330	3725-3732	NN	denotes	adaptor
T1329	3721-3724	DT	denotes	the
T1328	3718-3720	IN	denotes	of
T1327	3707-3717	NN	denotes	engagement
T1326	3704-3706	IN	denotes	in
T1281	3531-3533	NN	denotes	TB
T1280	3523-3530	JJ	denotes	primary
T1279	3520-3522	TO	denotes	to
T1278	3505-3519	NN	denotes	susceptibility
T1277	3495-3504	VB	denotes	increased
T1276	3492-3494	IN	denotes	as
T1275	3487-3491	RB	denotes	well
T1274	3484-3486	RB	denotes	as
T1273	3482-3483	-COMMA-	denotes	,
T1272	3470-3482	NN	denotes	reactivation
T1271	3466-3469	NN	denotes	MTb
T1270	3459-3465	JJ	denotes	latent
T1269	3456-3458	IN	denotes	of
T1268	3450-3455	NN	denotes	cause
T1267	3444-3449	JJ	denotes	major
T1266	3442-3443	DT	denotes	a
T1265	3439-3441	VB	denotes	is
T1264	3432-3438	PRP	denotes	itself
T1263	3422-3431	NN	denotes	infection
T1262	3416-3421	NN	denotes	HIV-1
T1261	3411-3415	IN	denotes	with
T1260	3400-3410	VB	denotes	associated
T1259	3389-3399	NN	denotes	compromise
T1258	3382-3388	JJ	denotes	immune
T1257	3370-3381	JJ	denotes	progressive
T1256	3366-3369	DT	denotes	the
T1255	3364-3365	-COMMA-	denotes	,
T1254	3353-3364	RB	denotes	Furthermore
T1253	3350-3351	-RRB-	denotes	)
T1252	3349-3350	-RRB-	denotes	]
T1251	3347-3349	CD	denotes	10
T1250	3346-3347	-LRB-	denotes	[
T1249	3345-3346	NN	denotes	–
T1248	3344-3345	-RRB-	denotes	]
T1247	3343-3344	CD	denotes	8
T1246	3342-3343	-LRB-	denotes	[
T1245	3339-3341	IN	denotes	in
T1244	3330-3338	VB	denotes	reviewed
T1243	3328-3329	-COMMA-	denotes	,
T1242	3327-3328	-RRB-	denotes	]
T1241	3326-3327	CD	denotes	7
T1240	3325-3326	-LRB-	denotes	[
T1239	3324-3325	-LRB-	denotes	(
T1238	3319-3323	NN	denotes	AIDS
T1237	3316-3318	TO	denotes	to
T1236	3304-3315	NN	denotes	progression
T1235	3300-3303	CC	denotes	and
T1234	3295-3299	NN	denotes	loss
T1233	3290-3294	NN	denotes	cell
T1232	3288-3289	NN	denotes	T
T1231	3284-3287	NN	denotes	CD4
T1230	3274-3283	VB	denotes	expedited
T1229	3272-3273	-COMMA-	denotes	,
T1228	3268-3272	NN	denotes	turn
T1227	3265-3267	IN	denotes	in
T1226	3263-3264	-COMMA-	denotes	,
T1225	3260-3263	CC	denotes	and
T1224	3254-3259	NN	denotes	loads
T1223	3248-3253	JJ	denotes	viral
T1222	3241-3247	JJ	denotes	higher
T1221	3238-3240	TO	denotes	to
T1220	3232-3237	VB	denotes	leads
T1219	3221-3231	RB	denotes	ultimately
T1218	3212-3220	NN	denotes	pathways
T1217	3200-3211	JJ	denotes	MTb-induced
T1216	3194-3199	DT	denotes	these
T1215	3190-3193	IN	denotes	via
T1214	3178-3189	NN	denotes	replication
T1213	3172-3177	NN	denotes	HIV-1
T1212	3169-3171	IN	denotes	of
T1211	3158-3168	NN	denotes	Activation
T1210	3155-3156	-RRB-	denotes	]
T1209	3154-3155	CD	denotes	6
T1208	3153-3154	-LRB-	denotes	[
T1207	3152-3153	NN	denotes	–
T1206	3151-3152	-RRB-	denotes	]
T1205	3150-3151	CD	denotes	4
T1204	3149-3150	-LRB-	denotes	[
T1203	3145-3148	NN	denotes	LTR
T1202	3139-3144	NN	denotes	HIV-1
T1201	3135-3138	DT	denotes	the
T1200	3132-3134	IN	denotes	of
T1199	3121-3131	NN	denotes	activation
T1198	3105-3120	JJ	denotes	transcriptional
T1197	3102-3104	IN	denotes	in
T1196	3095-3101	VB	denotes	result
T1195	3090-3094	RB	denotes	also
T1194	3085-3089	WDT	denotes	that
T1193	3079-3084	NN	denotes	cells
T1192	3069-3078	JJ	denotes	monocytic
T1191	3065-3068	CC	denotes	and
T1190	3059-3064	NN	denotes	cells
T1189	3057-3058	NN	denotes	T
T1188	3053-3056	NN	denotes	CD4
T1187	3050-3052	IN	denotes	in
T1186	3041-3049	NN	denotes	pathways
T1185	3028-3040	NN	denotes	transduction
T1184	3021-3027	NN	denotes	signal
T1183	3012-3020	VB	denotes	activate
T1182	3002-3011	NN	denotes	infection
T1181	2998-3001	NN	denotes	MTb
T1180	2995-2997	TO	denotes	to
T1179	2986-2994	NN	denotes	response
T1178	2983-2985	IN	denotes	in
T1177	2978-2982	NN	denotes	host
T1176	2972-2977	JJ	denotes	human
T1175	2968-2971	DT	denotes	the
T1174	2965-2967	IN	denotes	by
T1173	2956-2964	VB	denotes	produced
T1172	2945-2955	NN	denotes	chemokines
T1171	2941-2944	CC	denotes	and
T1170	2931-2940	NN	denotes	cytokines
T1169	2918-2930	JJ	denotes	inflammatory
T1168	2916-2917	-COMMA-	denotes	,
T1167	2905-2916	RB	denotes	Furthermore
T1166	2902-2903	-RRB-	denotes	)
T1165	2901-2902	-RRB-	denotes	]
T1164	2900-2901	CD	denotes	3
T1163	2899-2900	-LRB-	denotes	[
T1162	2896-2898	IN	denotes	in
T1161	2887-2895	VB	denotes	reviewed
T1160	2886-2887	-LRB-	denotes	(
T1159	2882-2885	NN	denotes	LTR
T1158	2873-2881	JJ	denotes	proviral
T1157	2869-2872	DT	denotes	the
T1156	2864-2868	IN	denotes	from
T1155	2850-2863	NN	denotes	transcription
T1154	2843-2849	VB	denotes	induce
T1153	2834-2842	RB	denotes	directly
T1152	2829-2833	WDT	denotes	that
T1151	2820-2828	NN	denotes	cascades
T1150	2810-2819	NN	denotes	signaling
T1149	2800-2809	VB	denotes	activates
T1148	2789-2799	NN	denotes	phagocytes
T1147	2777-2788	JJ	denotes	mononuclear
T1146	2774-2776	IN	denotes	on
T1145	2770-2773	NN	denotes	MTb
T1144	2767-2769	IN	denotes	by
T1143	2765-2766	-RRB-	denotes	)
T1142	2761-2765	NN	denotes	PRRs
T1141	2760-2761	-LRB-	denotes	(
T1140	2750-2759	NN	denotes	receptors
T1139	2738-2749	NN	denotes	recognition
T1138	2729-2737	NN	denotes	pathogen
T1137	2726-2728	IN	denotes	of
T1136	2715-2725	NN	denotes	engagement
T1135	2708-2714	JJ	denotes	Direct
T1134	2705-2706	-RRB-	denotes	]
T1133	2704-2705	CD	denotes	2
T1132	2703-2704	-LRB-	denotes	[
T1131	2701-2702	-COMMA-	denotes	,
T1130	2700-2701	-RRB-	denotes	]
T1129	2699-2700	CD	denotes	1
T1128	2698-2699	-LRB-	denotes	[
T1127	2696-2697	-RRB-	denotes	)
T1126	2691-2696	NN	denotes	HIV-1
T1125	2690-2691	-LRB-	denotes	(
T1124	2688-2689	CD	denotes	1
T1123	2683-2687	NN	denotes	type
T1122	2677-2682	NN	denotes	virus
T1121	2660-2676	NN	denotes	immunodeficiency
T1120	2654-2659	JJ	denotes	human
T1119	2649-2653	IN	denotes	with
T1118	2640-2648	VB	denotes	infected
T1117	2631-2639	NN	denotes	patients
T1116	2628-2630	IN	denotes	in
T1115	2622-2627	NN	denotes	death
T1114	2619-2621	IN	denotes	of
T1113	2613-2618	NN	denotes	cause
T1112	2609-2612	CC	denotes	and
T1111	2596-2608	NN	denotes	co-infection
T1110	2589-2595	JJ	denotes	common
T1109	2584-2588	RB	denotes	most
T1108	2580-2583	DT	denotes	the
T1107	2577-2579	VB	denotes	is
T1106	2575-2576	-COMMA-	denotes	,
T1105	2574-2575	-RRB-	denotes	)
T1104	2572-2574	NN	denotes	TB
T1103	2571-2572	-LRB-	denotes	(
T1102	2558-2570	NN	denotes	tuberculosis
T1101	2555-2557	IN	denotes	of
T1100	2549-2554	NN	denotes	agent
T1099	2539-2548	JJ	denotes	causative
T1098	2535-2538	DT	denotes	the
T1097	2533-2534	-COMMA-	denotes	,
T1096	2532-2533	-RRB-	denotes	)
T1095	2529-2532	NN	denotes	MTb
T1094	2528-2529	-LRB-	denotes	(
T1093	2515-2527	NN	denotes	tuberculosis
T1092	2501-2514	NN	denotes	Mycobacterium
T2846	6292-6294	IN	denotes	in
T2845	6283-6291	NNP	denotes	Hospital
T2844	6280-6282	POS	denotes	's
T2843	6272-6280	NNP	denotes	Children
T2842	6269-6271	IN	denotes	of
T1622	5183-5184	-COMMA-	denotes	,
T1621	5182-5183	NN	denotes	B
T1620	5174-5181	NN	denotes	subtype
T1619	5168-5173	NN	denotes	HIV-1
T1618	5153-5167	JJ	denotes	representative
T1617	5150-5152	IN	denotes	of
T1616	5138-5149	NN	denotes	replication
T1615	5125-5137	JJ	denotes	constitutive
T1614	5121-5124	IN	denotes	for
T1613	5112-5120	VB	denotes	required
T1612	5109-5111	VB	denotes	is
T1611	5105-5108	CC	denotes	and
T1610	5101-5104	NN	denotes	LTR
T1609	5097-5100	DT	denotes	the
T1608	5094-5096	IN	denotes	in
T1607	5087-5093	NN	denotes	motifs
T1606	5079-5086	NN	denotes	binding
T1605	5073-5078	NN	denotes	NF-κB
T1604	5068-5072	NN	denotes	core
T1603	5064-5067	DT	denotes	the
T1602	5055-5063	VB	denotes	overlaps
T1601	5050-5054	NN	denotes	site
T1600	5044-5049	NN	denotes	NFAT5
T1599	5039-5043	DT	denotes	This
T1598	5033-5037	NN	denotes	LTRs
T1597	5029-5032	NN	denotes	SIV
T1596	5025-5028	CC	denotes	and
T1595	5019-5024	NN	denotes	HIV-2
T1594	5016-5018	IN	denotes	in
T1593	5006-5015	VB	denotes	conserved
T1592	5001-5005	RB	denotes	also
T1591	4998-5000	VB	denotes	is
T1590	4994-4997	CC	denotes	and
T1589	4992-4993	-COMMA-	denotes	,
T1588	4984-4992	NN	denotes	subtypes
T1587	4978-4983	NN	denotes	HIV-1
T1586	4974-4977	DT	denotes	all
T1585	4967-4973	IN	denotes	across
T1584	4957-4966	VB	denotes	conserved
T1583	4950-4956	RB	denotes	highly
T1582	4947-4949	VB	denotes	is
T1581	4942-4946	WDT	denotes	that
T1580	4938-4941	NN	denotes	LTR
T1579	4932-4937	NN	denotes	HIV-1
T1578	4928-4931	DT	denotes	the
T1577	4921-4927	IN	denotes	within
T1576	4916-4920	NN	denotes	site
T1575	4914-4915	DT	denotes	a
T1574	4911-4913	TO	denotes	to
T1573	4905-4910	VB	denotes	binds
T1572	4903-4904	-COMMA-	denotes	,
T1571	4902-4903	-RRB-	denotes	)
T1570	4896-4902	NN	denotes	TonEBP
T1569	4893-4895	IN	denotes	as
T1568	4887-4892	VB	denotes	known
T1567	4882-4886	RB	denotes	also
T1566	4881-4882	-LRB-	denotes	(
T1565	4875-4880	NN	denotes	NFAT5
T1564	4873-4874	-COMMA-	denotes	,
T1563	4867-4873	NN	denotes	family
T1562	4865-4866	-RRB-	denotes	)
T1561	4861-4865	NN	denotes	NFAT
T1560	4860-4861	-LRB-	denotes	(
T1559	4854-4859	NN	denotes	cells
T1558	4852-4853	NN	denotes	T
T1557	4842-4851	VB	denotes	activated
T1556	4839-4841	IN	denotes	of
T1555	4832-4838	NN	denotes	factor
T1554	4824-4831	JJ	denotes	nuclear
T1553	4820-4823	DT	denotes	the
T1552	4817-4819	IN	denotes	of
T1551	4810-4816	NN	denotes	member
T1550	4799-4809	JJ	denotes	primordial
T1549	4794-4798	RB	denotes	most
T1548	4790-4793	DT	denotes	the
T1547	4785-4789	IN	denotes	that
T1546	4778-4784	VB	denotes	showed
T1545	4767-4777	RB	denotes	previously
T1544	4764-4766	PRP	denotes	We
T1543	4761-4762	-RRB-	denotes	]
T1542	4759-4761	CD	denotes	30
T1325	3696-3703	VB	denotes	results
T1324	3691-3695	NN	denotes	TLR2
T1323	3688-3690	IN	denotes	of
T1322	3677-3687	NN	denotes	Engagement
T1321	3674-3675	-RRB-	denotes	]
T1320	3672-3674	CD	denotes	20
T1319	3671-3672	-LRB-	denotes	[
T1318	3670-3671	CD	denotes	–
T1317	3669-3670	-RRB-	denotes	]
T1316	3667-3669	CD	denotes	16
T1315	3666-3667	-LRB-	denotes	[
T1314	3664-3665	CD	denotes	2
T1313	3662-3663	-RRB-	denotes	)
T1312	3659-3662	NN	denotes	TLR
T1311	3658-3659	-LRB-	denotes	(
T1310	3649-3657	NN	denotes	receptor
T1309	3639-3648	JJ	denotes	toll-like
T1308	3636-3638	VB	denotes	is
T1307	3626-3635	NN	denotes	infection
T1306	3622-3625	NN	denotes	MTb
T1305	3619-3621	IN	denotes	by
T1304	3609-3618	VB	denotes	triggered
T1303	3603-3608	NN	denotes	cells
T1302	3593-3602	JJ	denotes	monocytic
T1301	3590-3592	IN	denotes	on
T1300	3586-3589	NN	denotes	PRR
T1299	3578-3585	JJ	denotes	primary
T1298	3574-3577	DT	denotes	The
T1297	3571-3572	-RRB-	denotes	)
T1296	3570-3571	-RRB-	denotes	]
T1295	3569-3570	CD	denotes	8
T1294	3568-3569	-LRB-	denotes	[
T1293	3565-3567	IN	denotes	in
T1292	3556-3564	VB	denotes	reviewed
T1291	3554-3555	-COMMA-	denotes	,
T1290	3553-3554	-RRB-	denotes	]
T1289	3551-3553	CD	denotes	15
T1288	3550-3551	-LRB-	denotes	[
T1287	3549-3550	CD	denotes	–
T1286	3548-3549	-RRB-	denotes	]
T1285	3546-3548	CD	denotes	11
T1284	3545-3546	-LRB-	denotes	[
T1283	3544-3545	-LRB-	denotes	(
T1282	3534-3543	NN	denotes	infection
T410	1500-1510	NN	denotes	expression
T409	1495-1499	NN	denotes	gene
T408	1492-1494	IN	denotes	of
T407	1484-1491	NN	denotes	control
T406	1471-1483	JJ	denotes	MTb-mediated
T405	1467-1470	CC	denotes	and
T404	1462-1466	NN	denotes	TLR-
T403	1459-1461	IN	denotes	in
T402	1453-1458	NN	denotes	NFAT5
T401	1449-1452	IN	denotes	for
T400	1444-1448	NN	denotes	role
T399	1436-1443	JJ	denotes	general
T398	1434-1435	DT	denotes	a
T397	1422-1433	VB	denotes	demonstrate
T396	1417-1421	RB	denotes	also
T395	1408-1416	NN	denotes	findings
T394	1402-1407	DT	denotes	These
T393	1392-1400	NN	denotes	promoter
T392	1386-1391	JJ	denotes	viral
T391	1382-1385	DT	denotes	the
T390	1377-1381	IN	denotes	with
T389	1365-1376	NN	denotes	interaction
T388	1358-1364	JJ	denotes	direct
T387	1356-1357	DT	denotes	a
T386	1352-1355	IN	denotes	via
T385	1340-1351	NN	denotes	replication
T384	1334-1339	NN	denotes	HIV-1
T383	1331-1333	IN	denotes	of
T382	1320-1330	NN	denotes	regulation
T381	1316-1319	NN	denotes	MTb
T380	1313-1315	IN	denotes	in
T379	1308-1312	NN	denotes	role
T378	1300-1307	JJ	denotes	crucial
T377	1298-1299	DT	denotes	a
T376	1292-1297	VB	denotes	plays
T375	1286-1291	NN	denotes	NFAT5
T374	1282-1285	CC	denotes	and
T373	1280-1281	-COMMA-	denotes	,
T372	1270-1280	NN	denotes	expression
T371	1262-1269	NN	denotes	protein
T370	1258-1261	CC	denotes	and
T369	1253-1257	NN	denotes	gene
T368	1247-1252	NN	denotes	NFAT5
T367	1239-1246	VB	denotes	induces
T366	1229-1238	NN	denotes	infection
T365	1225-1228	NN	denotes	MTb
T364	1222-1224	TO	denotes	to
T363	1213-1221	NN	denotes	response
T362	1206-1212	JJ	denotes	immune
T361	1199-1205	JJ	denotes	innate
T360	1195-1198	DT	denotes	the
T359	1193-1194	-COMMA-	denotes	,
T358	1189-1193	RB	denotes	Thus
T357	1177-1187	NN	denotes	expression
T356	1172-1176	NN	denotes	gene
T355	1166-1171	NN	denotes	NFAT5
T354	1154-1165	JJ	denotes	MTb-induced
T353	1145-1153	VB	denotes	inhibits
T352	1131-1144	RB	denotes	significantly
T351	1129-1130	-COMMA-	denotes	,
T350	1124-1129	NN	denotes	TRAF6
T349	1120-1123	CC	denotes	and
T348	1118-1119	-COMMA-	denotes	,
T347	1113-1118	NN	denotes	IRAK1
T346	1111-1112	-COMMA-	denotes	,
T345	1106-1111	NN	denotes	MyD88
T344	1096-1105	NN	denotes	molecules
T343	1086-1095	VB	denotes	signaling
T342	1075-1085	JJ	denotes	downstream
T341	1071-1074	DT	denotes	the
T340	1061-1070	VB	denotes	including
T339	1059-1060	-COMMA-	denotes	,
T338	1050-1059	NN	denotes	monocytes
T337	1044-1049	JJ	denotes	human
T336	1041-1043	IN	denotes	in
T335	1033-1040	NN	denotes	pathway
T334	1029-1032	NN	denotes	TLR
T333	1025-1028	DT	denotes	the
T332	1022-1024	IN	denotes	of
T331	1011-1021	NN	denotes	components
T330	1007-1010	JJ	denotes	key
T329	1004-1006	IN	denotes	of
T328	999-1003	NN	denotes	RNAi
T327	996-998	IN	denotes	by
T326	986-995	NN	denotes	silencing
T325	984-985	-COMMA-	denotes	,
T324	973-984	RB	denotes	Furthermore
T323	968-971	NN	denotes	MTb
T322	965-967	IN	denotes	by
T321	953-964	NN	denotes	replication
T320	947-952	NN	denotes	HIV-1
T319	944-946	IN	denotes	of
T318	934-943	NN	denotes	induction
T317	925-933	JJ	denotes	efficent
T316	921-924	IN	denotes	for
T315	912-920	VB	denotes	required
T314	909-911	VB	denotes	is
T313	902-908	NN	denotes	clones
T312	892-901	JJ	denotes	molecular
T311	890-891	NN	denotes	C
T310	882-889	NN	denotes	subtype
T309	878-881	CC	denotes	and
T308	876-877	NN	denotes	B
T307	868-875	NN	denotes	subtype
T306	862-867	NN	denotes	HIV-1
T305	852-861	JJ	denotes	R5-tropic
T304	849-851	IN	denotes	of
T303	840-848	NN	denotes	promoter
T302	834-839	JJ	denotes	viral
T301	830-833	DT	denotes	the
T300	827-829	IN	denotes	in
T299	822-826	NN	denotes	site
T298	814-821	NN	denotes	binding
T297	808-813	NN	denotes	NFAT5
T296	801-807	JJ	denotes	intact
T295	798-800	DT	denotes	an
T294	793-797	IN	denotes	that
T293	789-792	CC	denotes	and
T292	787-788	-COMMA-	denotes	,
T291	781-787	NN	denotes	ligand
T290	779-780	-RRB-	denotes	)
T289	776-779	NN	denotes	TLR
T288	775-776	-LRB-	denotes	(
T287	766-774	NN	denotes	receptor
T286	756-765	JJ	denotes	Toll-like
T285	754-755	DT	denotes	a
T284	751-753	VB	denotes	is
T283	745-750	WDT	denotes	which
T282	743-744	-COMMA-	denotes	,
T281	740-743	NN	denotes	MTb
T280	737-739	IN	denotes	by
T279	729-736	VB	denotes	induced
T278	720-728	RB	denotes	strongly
T277	716-719	VB	denotes	are
T276	705-715	NN	denotes	expression
T275	697-704	NN	denotes	protein
T274	693-696	CC	denotes	and
T273	688-692	NN	denotes	gene
T272	682-687	NN	denotes	NFAT5
T271	677-681	IN	denotes	that
T270	672-676	VB	denotes	show
T269	669-671	PRP	denotes	We
T268	656-667	NN	denotes	macrophages
T267	644-655	JJ	denotes	co-infected
T266	641-643	IN	denotes	in
T265	629-640	NN	denotes	replication
T264	623-628	NN	denotes	HIV-1
T263	611-622	JJ	denotes	-stimulated
T262	610-611	-RRB-	denotes	)
T261	607-610	NN	denotes	MTb
T260	606-607	-LRB-	denotes	(
T259	593-605	NN	denotes	tuberculosis
T258	579-592	NN	denotes	Mycobacterium
T257	570-578	VB	denotes	inhibits
T256	568-569	-RRB-	denotes	)
T255	564-568	NN	denotes	RNAi
T254	563-564	-LRB-	denotes	(
T253	550-562	NN	denotes	interference
T252	546-549	NN	denotes	RNA
T251	543-545	IN	denotes	by
T250	541-542	-RRB-	denotes	)
T249	536-541	NN	denotes	NFAT5
T248	535-536	-LRB-	denotes	(
T247	533-534	CD	denotes	5
T246	527-532	NN	denotes	cells
T245	525-526	NN	denotes	T
T244	515-524	VB	denotes	activated
T243	512-514	IN	denotes	of
T242	505-511	NN	denotes	factor
T241	497-504	JJ	denotes	nuclear
T240	490-496	NN	denotes	factor
T239	476-489	NN	denotes	transcription
T238	472-475	DT	denotes	the
T237	469-471	IN	denotes	of
T236	458-468	NN	denotes	expression
T235	454-457	DT	denotes	the
T234	451-453	IN	denotes	of
T233	441-450	NN	denotes	silencing
T232	436-440	IN	denotes	that
T231	424-435	VB	denotes	demonstrate
T230	421-423	PRP	denotes	we
T229	419-420	-COMMA-	denotes	,
T228	415-419	RB	denotes	Here
T227	409-413	NN	denotes	load
T226	403-408	JJ	denotes	viral
T225	400-402	IN	denotes	in
T224	391-399	NN	denotes	increase
T223	388-390	DT	denotes	an
T222	385-387	TO	denotes	to
T221	377-384	VB	denotes	leading
T220	375-376	-COMMA-	denotes	,
T219	364-375	NN	denotes	replication
T218	358-363	NN	denotes	HIV-1
T217	348-357	VB	denotes	stimulate
T216	343-347	WDT	denotes	that
T215	334-342	NN	denotes	cascades
T214	324-333	NN	denotes	signaling
T213	317-323	JJ	denotes	immune
T212	308-316	JJ	denotes	adaptive
T211	304-307	CC	denotes	and
T210	297-303	JJ	denotes	innate
T209	286-296	VB	denotes	activating
T181	28-50	JJ	denotes	tuberculosis-Dependent
T182	51-56	NN	denotes	HIV-1
T183	57-70	NN	denotes	Transcription
T184	71-78	VB	denotes	Reveals
T185	79-80	DT	denotes	a
T186	81-84	NNP	denotes	New
T187	85-89	NNP	denotes	Role
T188	90-93	IN	denotes	for
T189	94-99	NN	denotes	NFAT5
T190	100-102	IN	denotes	in
T191	103-106	DT	denotes	the
T192	107-116	JJ	denotes	Toll-Like
T193	117-125	NN	denotes	Receptor
T194	126-133	NN	denotes	Pathway
T179	0-10	NN	denotes	Regulation
T180	11-27	NN	denotes	of Mycobacterium
T195	194-206	NN	denotes	Tuberculosis
T196	207-208	-LRB-	denotes	(
T197	208-210	NN	denotes	TB
T198	210-211	-RRB-	denotes	)
T199	212-219	NN	denotes	disease
T200	220-222	IN	denotes	in
T201	223-226	NN	denotes	HIV
T202	227-238	JJ	denotes	co-infected
T203	239-247	NN	denotes	patients
T204	248-259	VB	denotes	contributes
T205	260-262	TO	denotes	to
T206	263-272	VB	denotes	increased
T207	273-282	NN	denotes	mortality
T208	283-285	IN	denotes	by
T6713	15320-15331	JJ	denotes	significant
T6712	15309-15319	VB	denotes	considered
T6711	15305-15308	VB	denotes	was
T6710	15298-15304	NN	denotes	p≤0.05
T6709	15288-15297	NN	denotes	software.
T6708	15282-15287	NNP	denotes	Excel
T6707	15272-15281	NNP	denotes	Microsoft
T6706	15269-15271	IN	denotes	of
T6705	15265-15268	NN	denotes	aid
T6704	15261-15264	DT	denotes	the
T6703	15256-15260	IN	denotes	with
T6702	15249-15255	NN	denotes	t-Test
T6701	15241-15248	NN	denotes	Student
T6700	15234-15240	JJ	denotes	paired
T6699	15230-15233	DT	denotes	the
T6698	15224-15229	VB	denotes	using
T6697	15214-15223	VB	denotes	performed
T6696	15210-15213	VB	denotes	was
T6695	15203-15209	NN	denotes	groups
T6694	15199-15202	CD	denotes	two
T6693	15191-15198	IN	denotes	between
T6692	15180-15190	NN	denotes	Comparison
R45	T215	T214	arg1Of	cascades,signaling
R46	T215	T216	arg1Of	cascades,that
R47	T215	T217	arg1Of	cascades,stimulate
R48	T217	T220	arg1Of	stimulate,","
R49	T217	T221	modOf	stimulate,leading
R50	T219	T217	arg2Of	replication,stimulate
R51	T219	T218	arg1Of	replication,HIV-1
R52	T221	T222	arg1Of	leading,to
R53	T224	T222	arg2Of	increase,to
R54	T224	T223	arg1Of	increase,an
R55	T224	T225	arg1Of	increase,in
R56	T227	T225	arg2Of	load,in
R57	T227	T226	arg1Of	load,viral
R58	T230	T231	arg1Of	we,demonstrate
R59	T231	T228	arg1Of	demonstrate,Here
R60	T231	T229	arg1Of	demonstrate,","
R61	T233	T234	arg1Of	silencing,of
R62	T233	T257	arg1Of	silencing,inhibits
R63	T236	T234	arg2Of	expression,of
R64	T236	T235	arg1Of	expression,the
R65	T236	T237	arg1Of	expression,of
R66	T242	T237	arg2Of	factor,of
R67	T242	T238	arg1Of	factor,the
R68	T242	T239	arg1Of	factor,transcription
R69	T242	T240	arg1Of	factor,factor
R70	T242	T241	arg1Of	factor,nuclear
R71	T242	T243	arg1Of	factor,of
R72	T242	T251	arg1Of	factor,by
R73	T247	T243	arg2Of	5,of
R74	T247	T244	arg2Of	5,activated
R75	T247	T245	arg1Of	5,T
R76	T247	T246	arg1Of	5,cells
R77	T247	T248	arg1Of	5,(
R78	T249	T248	arg2Of	NFAT5,(
R79	T250	T248	arg3Of	),(
R80	T253	T251	arg2Of	interference,by
R81	T253	T252	arg1Of	interference,RNA
R82	T253	T254	arg1Of	interference,(
R83	T255	T254	arg2Of	RNAi,(
R84	T256	T254	arg3Of	),(
R85	T257	T231	arg2Of	inhibits,demonstrate
R86	T257	T232	arg1Of	inhibits,that
R87	T261	T260	arg2Of	MTb,(
R88	T262	T260	arg3Of	),(
R89	T265	T257	arg2Of	replication,inhibits
R90	T265	T258	arg1Of	replication,Mycobacterium
R91	T265	T259	arg1Of	replication,tuberculosis
R92	T265	T260	arg1Of	replication,(
R93	T265	T263	arg1Of	replication,-stimulated
R94	T265	T264	arg1Of	replication,HIV-1
R95	T265	T266	arg1Of	replication,in
R96	T268	T266	arg2Of	macrophages,in
R97	T268	T267	arg1Of	macrophages,co-infected
R98	T269	T270	arg1Of	We,show
R99	T273	T272	arg1Of	gene,NFAT5
R100	T273	T274	arg1Of	gene,and
R101	T274	T277	arg1Of	and,are
R102	T274	T279	arg2Of	and,induced
R103	T276	T274	arg2Of	expression,and
R104	T276	T275	arg1Of	expression,protein
R105	T279	T271	arg1Of	induced,that
R106	T279	T277	arg2Of	induced,are
R107	T279	T278	arg1Of	induced,strongly
R108	T279	T293	arg1Of	induced,and
R109	T281	T279	arg1Of	MTb,induced
R110	T281	T280	arg2Of	MTb,by
R111	T281	T282	arg1Of	MTb,","
R112	T281	T283	arg1Of	MTb,which
R113	T281	T284	arg1Of	MTb,is
R114	T289	T288	arg2Of	TLR,(
R115	T290	T288	arg3Of	),(
R116	T291	T284	arg2Of	ligand,is
R117	T291	T285	arg1Of	ligand,a
R118	T291	T286	arg1Of	ligand,Toll-like
R119	T291	T287	arg1Of	ligand,receptor
R120	T291	T288	arg1Of	ligand,(
R121	T293	T270	arg2Of	and,show
R122	T293	T292	arg1Of	and,","
R123	T294	T293	arg2Of	that,and
R124	T299	T295	arg1Of	site,an
R125	T299	T296	arg1Of	site,intact
R126	T299	T297	arg1Of	site,NFAT5
R127	T299	T298	arg1Of	site,binding
R128	T299	T300	arg1Of	site,in
R129	T299	T314	arg1Of	site,is
R130	T299	T315	arg2Of	site,required
R131	T303	T300	arg2Of	promoter,in
R132	T303	T301	arg1Of	promoter,the
R133	T303	T302	arg1Of	promoter,viral
R134	T303	T304	arg1Of	promoter,of
R135	T308	T309	arg1Of	B,and
R136	T310	T309	arg2Of	subtype,and
R137	T313	T304	arg2Of	clones,of
R138	T313	T305	arg1Of	clones,R5-tropic
R139	T313	T306	arg1Of	clones,HIV-1
R140	T313	T307	arg1Of	clones,subtype
R141	T313	T308	arg1Of	clones,B
R142	T313	T310	arg1Of	clones,subtype
R143	T313	T311	arg1Of	clones,C
R144	T313	T312	arg1Of	clones,molecular
R145	T315	T294	arg2Of	required,that
R146	T315	T314	arg2Of	required,is
R147	T315	T316	arg1Of	required,for
R148	T318	T316	arg2Of	induction,for
R149	T318	T317	arg1Of	induction,efficent
R150	T318	T319	arg1Of	induction,of
R151	T318	T322	arg1Of	induction,by
R152	T321	T319	arg2Of	replication,of
R153	T321	T320	arg1Of	replication,HIV-1
R154	T323	T322	arg2Of	MTb,by
R155	T326	T327	arg1Of	silencing,by
R156	T326	T329	arg1Of	silencing,of
R157	T326	T353	arg1Of	silencing,inhibits
R158	T328	T327	arg2Of	RNAi,by
R159	T331	T329	arg2Of	components,of
R160	T331	T330	arg1Of	components,key
R161	T331	T332	arg1Of	components,of
R162	T335	T332	arg2Of	pathway,of
R163	T335	T333	arg1Of	pathway,the
R164	T335	T334	arg1Of	pathway,TLR
R165	T335	T336	arg1Of	pathway,in
R166	T338	T336	arg2Of	monocytes,in
R167	T338	T337	arg1Of	monocytes,human
R168	T338	T339	arg1Of	monocytes,","
R169	T338	T340	arg1Of	monocytes,including
R170	T344	T341	arg1Of	molecules,the
R171	T344	T342	arg1Of	molecules,downstream
R172	T344	T343	arg1Of	molecules,signaling
R173	T345	T346	arg1Of	MyD88,","
R174	T346	T349	arg1Of	",",and
R175	T347	T346	arg2Of	IRAK1,","
R176	T349	T340	arg2Of	and,including
R177	T349	T344	arg1Of	and,molecules
R178	T349	T348	arg1Of	and,","
R179	T350	T349	arg2Of	TRAF6,and
R180	T353	T324	arg1Of	inhibits,Furthermore
R181	T353	T325	arg1Of	inhibits,","
R182	T353	T351	arg1Of	inhibits,","
R183	T353	T352	arg1Of	inhibits,significantly
R184	T357	T353	arg2Of	expression,inhibits
R185	T357	T354	arg1Of	expression,MTb-induced
R186	T357	T355	arg1Of	expression,NFAT5
R187	T357	T356	arg1Of	expression,gene
R188	T363	T360	arg1Of	response,the
R189	T363	T361	arg1Of	response,innate
R190	T363	T362	arg1Of	response,immune
R191	T363	T364	arg1Of	response,to
R192	T363	T367	arg1Of	response,induces
R193	T366	T364	arg2Of	infection,to
R194	T366	T365	arg1Of	infection,MTb
R195	T367	T358	arg1Of	induces,Thus
R196	T367	T359	arg1Of	induces,","
R197	T367	T374	arg1Of	induces,and
R198	T369	T370	arg1Of	gene,and
R199	T371	T370	arg2Of	protein,and
R200	T372	T367	arg2Of	expression,induces
R201	T372	T368	arg1Of	expression,NFAT5
R202	T372	T369	arg1Of	expression,gene
R203	T372	T371	arg1Of	expression,protein
R204	T374	T373	arg1Of	and,","
R205	T375	T376	arg1Of	NFAT5,plays
R206	T376	T374	arg2Of	plays,and
R207	T376	T380	arg1Of	plays,in
R208	T376	T386	arg1Of	plays,via
R209	T379	T376	arg2Of	role,plays
R210	T379	T377	arg1Of	role,a
R211	T379	T378	arg1Of	role,crucial
R212	T382	T380	arg2Of	regulation,in
R213	T382	T381	arg1Of	regulation,MTb
R214	T382	T383	arg1Of	regulation,of
R215	T385	T383	arg2Of	replication,of
R216	T385	T384	arg1Of	replication,HIV-1
R217	T389	T386	arg2Of	interaction,via
R218	T389	T387	arg1Of	interaction,a
R219	T389	T388	arg1Of	interaction,direct
R220	T389	T390	arg1Of	interaction,with
R221	T393	T390	arg2Of	promoter,with
R222	T393	T391	arg1Of	promoter,the
R223	T393	T392	arg1Of	promoter,viral
R224	T395	T394	arg1Of	findings,These
R225	T395	T397	arg1Of	findings,demonstrate
R226	T397	T396	arg1Of	demonstrate,also
R227	T400	T397	arg2Of	role,demonstrate
R228	T400	T398	arg1Of	role,a
R229	T400	T399	arg1Of	role,general
R230	T400	T401	arg1Of	role,for
R231	T400	T403	arg1Of	role,in
R232	T402	T401	arg2Of	NFAT5,for
R233	T404	T405	arg1Of	TLR-,and
R234	T406	T405	arg2Of	MTb-mediated,and
R235	T407	T403	arg2Of	control,in
R236	T407	T404	arg1Of	control,TLR-
R237	T407	T406	arg1Of	control,MTb-mediated
R238	T407	T408	arg1Of	control,of
R239	T410	T408	arg2Of	expression,of
R240	T410	T409	arg1Of	expression,gene
R663	T1093	T1092	arg1Of	tuberculosis,Mycobacterium
R664	T1093	T1094	arg1Of	tuberculosis,(
R665	T1093	T1097	arg1Of	tuberculosis,","
R666	T1093	T1107	arg1Of	tuberculosis,is
R667	T1095	T1094	arg2Of	MTb,(
R668	T1096	T1094	arg3Of	),(
R669	T1100	T1097	arg2Of	agent,","
R670	T1100	T1098	arg1Of	agent,the
R671	T1100	T1099	arg1Of	agent,causative
R672	T1100	T1101	arg1Of	agent,of
R673	T1102	T1101	arg2Of	tuberculosis,of
R674	T1102	T1103	arg1Of	tuberculosis,(
R675	T1104	T1103	arg2Of	TB,(
R676	T1105	T1103	arg3Of	),(
R677	T1107	T1106	arg1Of	is,","
R678	T1110	T1109	arg1Of	common,most
R679	T1111	T1112	arg1Of	co-infection,and
R680	T1112	T1107	arg2Of	and,is
R681	T1112	T1108	arg1Of	and,the
R682	T1112	T1110	arg1Of	and,common
R683	T1112	T1114	arg1Of	and,of
R684	T1113	T1112	arg2Of	cause,and
R685	T1115	T1114	arg2Of	death,of
R686	T1115	T1116	arg1Of	death,in
R687	T1117	T1116	arg2Of	patients,in
R688	T1117	T1118	arg2Of	patients,infected
R689	T1118	T1119	arg1Of	infected,with
R690	T1123	T1119	arg2Of	type,with
R691	T1123	T1120	arg1Of	type,human
R692	T1123	T1121	arg1Of	type,immunodeficiency
R693	T1123	T1122	arg1Of	type,virus
R694	T1123	T1124	arg1Of	type,1
R695	T1123	T1125	arg1Of	type,(
R696	T1123	T1128	arg1Of	type,[
R697	T1123	T1131	arg1Of	type,","
R698	T1123	T1132	arg1Of	type,[
R699	T1126	T1125	arg2Of	HIV-1,(
R700	T1127	T1125	arg3Of	),(
R701	T1129	T1128	arg2Of	1,[
R702	T1130	T1128	arg3Of	],[
R703	T1133	T1132	arg2Of	2,[
R704	T1134	T1132	arg3Of	],[
R705	T1136	T1135	arg1Of	engagement,Direct
R706	T1136	T1137	arg1Of	engagement,of
R707	T1136	T1144	arg1Of	engagement,by
R708	T1136	T1146	arg1Of	engagement,on
R709	T1136	T1149	arg1Of	engagement,activates
R710	T1140	T1137	arg2Of	receptors,of
R711	T1140	T1138	arg1Of	receptors,pathogen
R712	T1140	T1139	arg1Of	receptors,recognition
R713	T1140	T1141	arg1Of	receptors,(
R714	T1142	T1141	arg2Of	PRRs,(
R715	T1143	T1141	arg3Of	),(
R716	T1145	T1144	arg2Of	MTb,by
R717	T1148	T1146	arg2Of	phagocytes,on
R718	T1148	T1147	arg1Of	phagocytes,mononuclear
R719	T1151	T1149	arg2Of	cascades,activates
R720	T1151	T1150	arg1Of	cascades,signaling
R721	T1151	T1152	arg1Of	cascades,that
R722	T1151	T1154	arg1Of	cascades,induce
R723	T1154	T1153	arg1Of	induce,directly
R724	T1155	T1154	arg2Of	transcription,induce
R725	T1155	T1156	arg1Of	transcription,from
R726	T1155	T1160	arg1Of	transcription,(
R727	T1159	T1156	arg2Of	LTR,from
R728	T1159	T1157	arg1Of	LTR,the
R729	T1159	T1158	arg1Of	LTR,proviral
R730	T1161	T1160	arg2Of	reviewed,(
R731	T1161	T1162	arg1Of	reviewed,in
R732	T1161	T1163	arg1Of	reviewed,[
R733	T1164	T1163	arg2Of	3,[
R734	T1165	T1163	arg3Of	],[
R735	T1166	T1160	arg3Of	),(
R736	T1170	T1171	arg1Of	cytokines,and
R737	T1171	T1169	arg1Of	and,inflammatory
R738	T1171	T1173	arg2Of	and,produced
R739	T1171	T1183	arg1Of	and,activate
R740	T1172	T1171	arg2Of	chemokines,and
R741	T1173	T1178	arg1Of	produced,in
R742	T1177	T1173	arg1Of	host,produced
R743	T1177	T1174	arg2Of	host,by
R744	T1177	T1175	arg1Of	host,the
R745	T1177	T1176	arg1Of	host,human
R746	T1178	T1180	arg1Of	in,to
R747	T1179	T1178	arg2Of	response,in
R748	T1182	T1178	arg3Of	infection,in
R749	T1182	T1181	arg1Of	infection,MTb
R750	T1183	T1167	arg1Of	activate,Furthermore
R751	T1183	T1168	arg1Of	activate,","
R752	T1186	T1183	arg2Of	pathways,activate
R753	T1186	T1184	arg1Of	pathways,signal
R754	T1186	T1185	arg1Of	pathways,transduction
R755	T1186	T1187	arg1Of	pathways,in
R756	T1186	T1194	arg1Of	pathways,that
R757	T1186	T1196	arg1Of	pathways,result
R758	T1190	T1188	arg1Of	cells,CD4
R759	T1190	T1189	arg1Of	cells,T
R760	T1190	T1191	arg1Of	cells,and
R761	T1191	T1187	arg2Of	and,in
R762	T1193	T1191	arg2Of	cells,and
R763	T1193	T1192	arg1Of	cells,monocytic
R764	T1196	T1195	arg1Of	result,also
R765	T1196	T1197	arg1Of	result,in
R766	T1196	T1208	arg1Of	result,[
R767	T1199	T1197	arg2Of	activation,in
R768	T1199	T1198	arg1Of	activation,transcriptional
R769	T1199	T1200	arg1Of	activation,of
R770	T1205	T1204	arg2Of	4,[
R771	T1206	T1204	arg3Of	],[
R772	T1207	T1200	arg2Of	–,of
R773	T1207	T1201	arg1Of	–,the
R774	T1207	T1202	arg1Of	–,HIV-1
R775	T1207	T1203	arg1Of	–,LTR
R776	T1207	T1204	arg1Of	–,[
R777	T1209	T1208	arg2Of	6,[
R778	T1210	T1208	arg3Of	],[
R779	T1211	T1212	arg1Of	Activation,of
R780	T1211	T1215	arg1Of	Activation,via
R781	T1211	T1220	arg1Of	Activation,leads
R782	T1211	T1230	arg1Of	Activation,expedited
R783	T1214	T1212	arg2Of	replication,of
R784	T1214	T1213	arg1Of	replication,HIV-1
R785	T1218	T1215	arg2Of	pathways,via
R786	T1218	T1216	arg1Of	pathways,these
R787	T1218	T1217	arg1Of	pathways,MTb-induced
R788	T1220	T1221	arg1Of	leads,to
R789	T1220	T1226	arg1Of	leads,","
R790	T1221	T1225	arg1Of	to,and
R791	T1224	T1221	arg2Of	loads,to
R792	T1224	T1222	arg1Of	loads,higher
R793	T1224	T1223	arg1Of	loads,viral
R794	T1226	T1219	arg1Of	",",ultimately
R795	T1228	T1227	arg2Of	turn,in
R796	T1230	T1226	arg2Of	expedited,","
R797	T1230	T1227	arg1Of	expedited,in
R798	T1230	T1229	arg1Of	expedited,","
R799	T1230	T1250	arg1Of	expedited,[
R800	T1230	T1253	arg1Of	expedited,)
R801	T1234	T1231	arg1Of	loss,CD4
R802	T1234	T1232	arg1Of	loss,T
R803	T1234	T1233	arg1Of	loss,cell
R804	T1234	T1235	arg1Of	loss,and
R805	T1235	T1230	arg2Of	and,expedited
R806	T1235	T1243	arg1Of	and,","
R807	T1235	T1244	arg2Of	and,reviewed
R808	T1236	T1235	arg2Of	progression,and
R809	T1236	T1237	arg1Of	progression,to
R810	T1238	T1237	arg2Of	AIDS,to
R811	T1238	T1239	arg1Of	AIDS,(
R812	T1241	T1239	arg2Of	7,(
R813	T1241	T1240	arg1Of	7,[
R814	T1242	T1239	arg3Of	],(
R815	T1244	T1245	arg1Of	reviewed,in
R816	T1247	T1246	arg2Of	8,[
R817	T1248	T1246	arg3Of	],[
R818	T1249	T1245	arg2Of	–,in
R819	T1249	T1246	arg1Of	–,[
R820	T1251	T1250	arg2Of	10,[
R821	T1252	T1250	arg3Of	],[
R822	T1259	T1256	arg1Of	compromise,the
R823	T1259	T1257	arg1Of	compromise,progressive
R824	T1259	T1258	arg1Of	compromise,immune
R825	T1259	T1260	arg2Of	compromise,associated
R826	T1259	T1265	arg1Of	compromise,is
R827	T1260	T1261	arg1Of	associated,with
R828	T1263	T1261	arg2Of	infection,with
R829	T1263	T1262	arg1Of	infection,HIV-1
R830	T1263	T1264	arg1Of	infection,itself
R831	T1265	T1254	arg1Of	is,Furthermore
R832	T1265	T1255	arg1Of	is,","
R833	T1268	T1265	arg2Of	cause,is
R834	T1268	T1266	arg1Of	cause,a
R835	T1268	T1267	arg1Of	cause,major
R836	T1268	T1269	arg1Of	cause,of
R837	T1272	T1270	arg1Of	reactivation,latent
R838	T1272	T1271	arg1Of	reactivation,MTb
R839	T1272	T1276	arg1Of	reactivation,as
R840	T1276	T1269	arg2Of	as,of
R841	T1276	T1273	arg1Of	as,","
R842	T1276	T1274	arg1Of	as,as
R843	T1276	T1275	arg1Of	as,well
R844	T1278	T1276	arg2Of	susceptibility,as
R845	T1278	T1277	arg2Of	susceptibility,increased
R846	T1278	T1279	arg1Of	susceptibility,to
R847	T1282	T1279	arg2Of	infection,to
R848	T1282	T1280	arg1Of	infection,primary
R849	T1282	T1281	arg1Of	infection,TB
R850	T1282	T1283	arg1Of	infection,(
R851	T1285	T1284	arg2Of	11,[
R852	T1286	T1284	arg3Of	],[
R853	T1287	T1283	arg2Of	–,(
R854	T1287	T1284	arg1Of	–,[
R855	T1287	T1288	arg1Of	–,[
R856	T1287	T1291	arg1Of	–,","
R857	T1287	T1292	arg2Of	–,reviewed
R858	T1289	T1288	arg2Of	15,[
R859	T1290	T1288	arg3Of	],[
R860	T1292	T1293	arg1Of	reviewed,in
R861	T1295	T1293	arg2Of	8,in
R862	T1295	T1294	arg2Of	8,[
R863	T1296	T1294	arg3Of	],[
R864	T1297	T1283	arg3Of	),(
R865	T1300	T1298	arg1Of	PRR,The
R866	T1300	T1299	arg1Of	PRR,primary
R867	T1300	T1301	arg1Of	PRR,on
R868	T1300	T1308	arg1Of	PRR,is
R869	T1303	T1301	arg2Of	cells,on
R870	T1303	T1302	arg1Of	cells,monocytic
R871	T1303	T1304	arg2Of	cells,triggered
R872	T1307	T1304	arg1Of	infection,triggered
R873	T1307	T1305	arg2Of	infection,by
R874	T1307	T1306	arg1Of	infection,MTb
R875	T1308	T1319	arg1Of	is,[
R876	T1310	T1309	arg1Of	receptor,toll-like
R877	T1310	T1311	arg1Of	receptor,(
R878	T1310	T1314	arg1Of	receptor,2
R879	T1312	T1311	arg2Of	TLR,(
R880	T1313	T1311	arg3Of	),(
R881	T1316	T1315	arg2Of	16,[
R882	T1317	T1315	arg3Of	],[
R883	T1318	T1308	arg2Of	–,is
R884	T1318	T1310	arg1Of	–,receptor
R885	T1318	T1315	arg1Of	–,[
R886	T1320	T1319	arg2Of	20,[
R887	T1321	T1319	arg3Of	],[
R888	T1322	T1323	arg1Of	Engagement,of
R889	T1322	T1325	arg1Of	Engagement,results
R890	T1324	T1323	arg2Of	TLR2,of
R891	T1325	T1326	arg1Of	results,in
R892	T1327	T1326	arg2Of	engagement,in
R893	T1327	T1328	arg1Of	engagement,of
R894	T1332	T1329	arg1Of	MyD88,the
R895	T1332	T1330	arg1Of	MyD88,adaptor
R896	T1332	T1331	arg1Of	MyD88,protein
R897	T1332	T1333	arg1Of	MyD88,and
R898	T1333	T1328	arg2Of	and,of
R899	T1336	T1333	arg2Of	recruitment,and
R900	T1336	T1334	arg1Of	recruitment,the
R901	T1336	T1335	arg1Of	recruitment,subsequent
R902	T1336	T1337	arg1Of	recruitment,of
R903	T1339	T1337	arg2Of	kinases,of
R904	T1339	T1338	arg1Of	kinases,several
R905	T1339	T1340	arg1Of	kinases,","
R906	T1339	T1341	arg1Of	kinases,including
R907	T1342	T1343	arg1Of	IRAK1,and
R908	T1343	T1346	arg1Of	and,and
R909	T1344	T1343	arg2Of	IRAK4,and
R910	T1346	T1341	arg2Of	and,including
R911	T1346	T1345	arg1Of	and,","
R912	T1350	T1346	arg2Of	TRAF6,and
R913	T1350	T1347	arg1Of	TRAF6,the
R914	T1350	T1348	arg1Of	TRAF6,ubiquitin
R915	T1350	T1349	arg1Of	TRAF6,ligase
R916	T1350	T1351	arg1Of	TRAF6,(
R917	T1353	T1352	arg2Of	21,[
R918	T1354	T1352	arg3Of	],[
R919	T1355	T1351	arg2Of	–,(
R920	T1355	T1352	arg1Of	–,[
R921	T1355	T1356	arg1Of	–,[
R922	T1355	T1359	arg1Of	–,","
R923	T1355	T1360	arg2Of	–,reviewed
R924	T1355	T1365	arg1Of	–,","
R925	T1355	T1366	arg1Of	–,[
R926	T1357	T1356	arg2Of	23,[
R927	T1358	T1356	arg3Of	],[
R928	T1360	T1361	arg1Of	reviewed,in
R929	T1363	T1361	arg2Of	10,in
R930	T1363	T1362	arg2Of	10,[
R931	T1364	T1362	arg3Of	],[
R932	T1367	T1366	arg2Of	24,[
R933	T1368	T1366	arg3Of	],[
R934	T1369	T1351	arg3Of	),(
R935	T1370	T1371	arg1Of	TRAF6,activates
R936	T1371	T1424	arg1Of	activates,","
R937	T1371	T1425	arg1Of	activates,[
R938	T1371	T1438	arg1Of	activates,)
R939	T1373	T1372	arg1Of	kinase,IκB
R940	T1373	T1374	arg1Of	kinase,(
R941	T1373	T1377	arg1Of	kinase,and
R942	T1375	T1374	arg2Of	IKK,(
R943	T1376	T1374	arg3Of	),(
R944	T1377	T1371	arg2Of	and,activates
R945	T1381	T1380	arg2Of	MAP,(
R946	T1382	T1380	arg3Of	),(
R947	T1383	T1377	arg2Of	kinases,and
R948	T1383	T1378	arg1Of	kinases,mitogen-activated
R949	T1383	T1379	arg1Of	kinases,protein
R950	T1383	T1380	arg1Of	kinases,(
R951	T1383	T1384	arg1Of	kinases,that
R952	T1383	T1390	arg1Of	kinases,induce
R953	T1387	T1386	arg2Of	turn,in
R954	T1390	T1385	arg1Of	induce,","
R955	T1390	T1386	arg1Of	induce,in
R956	T1390	T1388	arg1Of	induce,","
R957	T1390	T1389	arg1Of	induce,ultimately
R958	T1391	T1390	arg2Of	activation,induce
R959	T1391	T1392	arg1Of	activation,of
R960	T1396	T1392	arg2Of	families,of
R961	T1396	T1393	arg1Of	families,specific
R962	T1396	T1394	arg1Of	families,transcription
R963	T1396	T1395	arg1Of	families,factor
R964	T1396	T1397	arg1Of	families,","
R965	T1396	T1398	arg1Of	families,including
R966	T1400	T1401	arg1Of	NF-κB,and
R967	T1402	T1401	arg2Of	AP-1,and
R968	T1403	T1398	arg2Of	families,including
R969	T1403	T1399	arg1Of	families,the
R970	T1403	T1400	arg1Of	families,NF-κB
R971	T1403	T1402	arg1Of	families,AP-1
R972	T1403	T1404	arg1Of	families,","
R973	T1403	T1405	arg1Of	families,which
R974	T1403	T1406	arg1Of	families,have
R975	T1403	T1407	arg1Of	families,been
R976	T1403	T1408	arg2Of	families,shown
R977	T1403	T1410	arg1Of	families,associate
R978	T1403	T1417	arg1Of	families,drive
R979	T1408	T1406	arg2Of	shown,have
R980	T1408	T1407	arg2Of	shown,been
R981	T1410	T1409	arg1Of	associate,to
R982	T1410	T1411	arg1Of	associate,with
R983	T1410	T1415	arg1Of	associate,and
R984	T1414	T1411	arg2Of	LTR,with
R985	T1414	T1412	arg1Of	LTR,the
R986	T1414	T1413	arg1Of	LTR,HIV-1
R987	T1415	T1408	arg3Of	and,shown
R988	T1417	T1415	arg2Of	drive,and
R989	T1417	T1416	arg1Of	drive,to
R990	T1419	T1417	arg2Of	transcription,drive
R991	T1419	T1418	arg1Of	transcription,its
R992	T1419	T1420	arg1Of	transcription,(
R993	T1422	T1420	arg2Of	22,(
R994	T1422	T1421	arg1Of	22,[
R995	T1423	T1420	arg3Of	],(
R996	T1426	T1425	arg2Of	25,[
R997	T1427	T1425	arg3Of	],[
R998	T1428	T1371	arg3Of	–,activates
R999	T1428	T1429	arg1Of	–,[
R1000	T1428	T1432	arg1Of	–,","
R1001	T1428	T1433	arg2Of	–,reviewed
R1002	T1430	T1429	arg2Of	27,[
R1003	T1431	T1429	arg3Of	],[
R1004	T1433	T1434	arg1Of	reviewed,in
R1005	T1436	T1434	arg2Of	10,in
R1006	T1436	T1435	arg2Of	10,[
R1007	T1437	T1435	arg3Of	],[
R1008	T1441	T1442	arg1Of	HIV-1,comprises
R1009	T1442	T1446	arg1Of	comprises,and
R1010	T1444	T1442	arg2Of	subtypes,comprises
R1011	T1444	T1443	arg1Of	subtypes,several
R1012	T1446	T1439	arg1Of	and,Notably
R1013	T1446	T1440	arg1Of	and,","
R1014	T1446	T1445	arg1Of	and,","
R1015	T1448	T1447	arg1Of	LTR,the
R1016	T1448	T1449	arg1Of	LTR,of
R1017	T1448	T1452	arg1Of	LTR,is
R1018	T1448	T1453	arg1Of	LTR,unique
R1019	T1451	T1449	arg2Of	subtype,of
R1020	T1451	T1450	arg1Of	subtype,each
R1021	T1452	T1446	arg2Of	is,and
R1022	T1453	T1452	arg2Of	unique,is
R1023	T1453	T1454	arg1Of	unique,with
R1024	T1455	T1454	arg2Of	respect,with
R1025	T1455	T1456	arg1Of	respect,to
R1026	T1458	T1459	arg1Of	number,and
R1027	T1459	T1456	arg2Of	and,to
R1028	T1459	T1457	arg1Of	and,the
R1029	T1459	T1461	arg1Of	and,of
R1030	T1460	T1459	arg2Of	organization,and
R1031	T1464	T1461	arg2Of	sites,of
R1032	T1464	T1462	arg1Of	sites,activator
R1033	T1464	T1463	arg1Of	sites,binding
R1034	T1466	T1465	arg2Of	example,For
R1035	T1470	T1468	arg1Of	B,HIV-1
R1036	T1470	T1469	arg1Of	B,subtype
R1037	T1470	T1471	arg1Of	B,","
R1038	T1470	T1478	arg1Of	B,and
R1039	T1474	T1473	arg1Of	highly,most
R1040	T1475	T1474	arg1Of	characterized,highly
R1041	T1477	T1471	arg2Of	subtype,","
R1042	T1477	T1472	arg1Of	subtype,the
R1043	T1477	T1475	arg1Of	subtype,characterized
R1044	T1477	T1476	arg1Of	subtype,viral
R1045	T1478	T1495	arg1Of	and,has
R1046	T1481	T1479	arg1Of	cause,the
R1047	T1481	T1480	arg1Of	cause,primary
R1048	T1481	T1482	arg1Of	cause,of
R1049	T1481	T1484	arg1Of	cause,in
R1050	T1481	T1487	arg1Of	cause,","
R1051	T1483	T1482	arg2Of	infection,of
R1052	T1486	T1484	arg2Of	Americas,in
R1053	T1486	T1485	arg1Of	Americas,the
R1054	T1487	T1478	arg2Of	",",and
R1055	T1488	T1489	arg1Of	Europe,","
R1056	T1489	T1492	arg1Of	",",and
R1057	T1490	T1489	arg2Of	Japan,","
R1058	T1492	T1487	arg2Of	and,","
R1059	T1492	T1491	arg1Of	and,","
R1060	T1493	T1492	arg2Of	Australia,and
R1061	T1495	T1465	arg1Of	has,For
R1062	T1495	T1467	arg1Of	has,","
R1063	T1495	T1494	arg1Of	has,","
R1064	T1499	T1495	arg2Of	motifs,has
R1065	T1499	T1496	arg1Of	motifs,two
R1066	T1499	T1497	arg1Of	motifs,tandem
R1067	T1499	T1498	arg1Of	motifs,NF-κB
R1068	T1499	T1500	arg1Of	motifs,in
R1069	T1502	T1500	arg2Of	LTR,in
R1070	T1502	T1501	arg1Of	LTR,its
R1071	T1504	T1503	arg2Of	contrast,By
R1072	T1508	T1509	arg1Of	C,and
R1073	T1509	T1506	arg1Of	and,HIV-1
R1074	T1509	T1507	arg1Of	and,subtypes
R1075	T1509	T1511	arg1Of	and,","
R1076	T1509	T1512	arg1Of	and,which
R1077	T1509	T1513	arg1Of	and,have
R1078	T1509	T1514	arg1Of	and,spread
R1079	T1509	T1524	arg1Of	and,have
R1080	T1510	T1509	arg2Of	E,and
R1081	T1514	T1513	arg2Of	spread,have
R1082	T1514	T1515	arg1Of	spread,disproportionately
R1083	T1514	T1516	arg1Of	spread,in
R1084	T1519	T1520	arg1Of	Africa,and
R1085	T1520	T1516	arg2Of	and,in
R1086	T1520	T1517	arg1Of	and,TB-burdened
R1087	T1520	T1518	arg1Of	and,sub-Saharan
R1088	T1522	T1520	arg2Of	Asia,and
R1089	T1522	T1521	arg1Of	Asia,southeast
R1090	T1524	T1503	arg1Of	have,By
R1091	T1524	T1505	arg1Of	have,","
R1092	T1524	T1523	arg1Of	have,","
R1093	T1524	T1541	arg1Of	have,[
R1094	T1525	T1526	arg1Of	three,and
R1095	T1527	T1526	arg2Of	one,and
R1096	T1530	T1524	arg2Of	sites,have
R1097	T1530	T1525	arg1Of	sites,three
R1098	T1530	T1527	arg1Of	sites,one
R1099	T1530	T1528	arg1Of	sites,NF-κB
R1100	T1530	T1529	arg1Of	sites,binding
R1101	T1530	T1531	arg1Of	sites,","
R1102	T1530	T1532	arg1Of	sites,respectively
R1103	T1530	T1533	arg1Of	sites,[
R1104	T1530	T1536	arg1Of	sites,","
R1105	T1534	T1533	arg2Of	1,[
R1106	T1535	T1533	arg3Of	],[
R1107	T1538	T1537	arg2Of	28,[
R1108	T1539	T1537	arg3Of	],[
R1109	T1540	T1536	arg2Of	–,","
R1110	T1540	T1537	arg1Of	–,[
R1111	T1542	T1541	arg2Of	30,[
R1112	T1543	T1541	arg3Of	],[
R1113	T1544	T1546	arg1Of	We,showed
R1114	T1546	T1545	arg1Of	showed,previously
R1115	T1550	T1549	arg1Of	primordial,most
R1116	T1551	T1548	arg1Of	member,the
R1117	T1551	T1550	arg1Of	member,primordial
R1118	T1551	T1552	arg1Of	member,of
R1119	T1551	T1573	arg1Of	member,binds
R1120	T1561	T1560	arg2Of	NFAT,(
R1121	T1562	T1560	arg3Of	),(
R1122	T1563	T1552	arg2Of	family,of
R1123	T1563	T1553	arg1Of	family,the
R1124	T1563	T1554	arg1Of	family,nuclear
R1125	T1563	T1555	arg1Of	family,factor
R1126	T1563	T1556	arg1Of	family,of
R1127	T1563	T1557	arg2Of	family,activated
R1128	T1563	T1558	arg1Of	family,T
R1129	T1563	T1559	arg1Of	family,cells
R1130	T1563	T1560	arg1Of	family,(
R1131	T1563	T1564	arg1Of	family,","
R1132	T1565	T1564	arg2Of	NFAT5,","
R1133	T1565	T1566	arg1Of	NFAT5,(
R1134	T1568	T1566	arg2Of	known,(
R1135	T1568	T1567	arg1Of	known,also
R1136	T1568	T1569	arg1Of	known,as
R1137	T1570	T1569	arg2Of	TonEBP,as
R1138	T1571	T1566	arg3Of	),(
R1139	T1573	T1546	arg2Of	binds,showed
R1140	T1573	T1547	arg1Of	binds,that
R1141	T1573	T1572	arg1Of	binds,","
R1142	T1573	T1574	arg1Of	binds,to
R1143	T1576	T1574	arg2Of	site,to
R1144	T1576	T1575	arg1Of	site,a
R1145	T1576	T1577	arg1Of	site,within
R1146	T1576	T1581	arg1Of	site,that
R1147	T1576	T1582	arg1Of	site,is
R1148	T1576	T1584	arg2Of	site,conserved
R1149	T1576	T1591	arg1Of	site,is
R1150	T1576	T1593	arg2Of	site,conserved
R1151	T1580	T1577	arg2Of	LTR,within
R1152	T1580	T1578	arg1Of	LTR,the
R1153	T1580	T1579	arg1Of	LTR,HIV-1
R1154	T1584	T1582	arg2Of	conserved,is
R1155	T1584	T1583	arg1Of	conserved,highly
R1156	T1584	T1585	arg1Of	conserved,across
R1157	T1584	T1590	arg1Of	conserved,and
R1158	T1588	T1585	arg2Of	subtypes,across
R1159	T1588	T1586	arg1Of	subtypes,all
R1160	T1588	T1587	arg1Of	subtypes,HIV-1
R1161	T1590	T1589	arg1Of	and,","
R1162	T1593	T1590	arg2Of	conserved,and
R1163	T1593	T1591	arg2Of	conserved,is
R1164	T1593	T1592	arg1Of	conserved,also
R1165	T1593	T1594	arg1Of	conserved,in
R1166	T1595	T1596	arg1Of	HIV-2,and
R1167	T1597	T1596	arg2Of	SIV,and
R1168	T1598	T1594	arg2Of	LTRs,in
R1169	T1598	T1595	arg1Of	LTRs,HIV-2
R1170	T1598	T1597	arg1Of	LTRs,SIV
R1171	T1601	T1599	arg1Of	site,This
R1172	T1601	T1600	arg1Of	site,NFAT5
R1173	T1601	T1602	arg1Of	site,overlaps
R1174	T1601	T1612	arg1Of	site,is
R1175	T1601	T1613	arg2Of	site,required
R1176	T1602	T1608	arg1Of	overlaps,in
R1177	T1602	T1611	arg1Of	overlaps,and
R1178	T1607	T1602	arg2Of	motifs,overlaps
R1179	T1607	T1603	arg1Of	motifs,the
R1180	T1607	T1604	arg1Of	motifs,core
R1181	T1607	T1605	arg1Of	motifs,NF-κB
R1182	T1607	T1606	arg1Of	motifs,binding
R1183	T1610	T1608	arg2Of	LTR,in
R1184	T1610	T1609	arg1Of	LTR,the
R1185	T1613	T1611	arg2Of	required,and
R1186	T1613	T1612	arg2Of	required,is
R1187	T1613	T1614	arg1Of	required,for
R1188	T1613	T1636	arg1Of	required,[
R1189	T1616	T1614	arg2Of	replication,for
R1190	T1616	T1615	arg1Of	replication,constitutive
R1191	T1616	T1617	arg1Of	replication,of
R1192	T1616	T1628	arg1Of	replication,in
R1193	T1621	T1622	arg1Of	B,","
R1194	T1622	T1625	arg1Of	",",and
R1195	T1623	T1622	arg2Of	C,","
R1196	T1625	T1624	arg1Of	and,","
R1197	T1626	T1625	arg2Of	E,and
R1198	T1627	T1617	arg2Of	isolates,of
R1199	T1627	T1618	arg1Of	isolates,representative
R1200	T1627	T1619	arg1Of	isolates,HIV-1
R1201	T1627	T1620	arg1Of	isolates,subtype
R1202	T1627	T1621	arg1Of	isolates,B
R1203	T1627	T1623	arg1Of	isolates,C
R1204	T1627	T1626	arg1Of	isolates,E
R1205	T1632	T1628	arg2Of	macrophages,in
R1206	T1632	T1629	arg1Of	macrophages,human
R1207	T1632	T1630	arg1Of	macrophages,primary
R1208	T1632	T1631	arg1Of	macrophages,monocyte-derived
R1209	T1632	T1633	arg1Of	macrophages,(
R1210	T1634	T1633	arg2Of	MDM,(
R1211	T1635	T1633	arg3Of	),(
R1212	T1637	T1636	arg2Of	31,[
R1213	T1638	T1636	arg3Of	],[
R1214	T1641	T1639	arg2Of	NFAT5,Given
R1215	T1641	T1640	arg1Of	NFAT5,that
R1216	T1641	T1642	arg1Of	NFAT5,has
R1217	T1641	T1644	arg1Of	NFAT5,been
R1218	T1641	T1645	arg2Of	NFAT5,shown
R1219	T1641	T1647	arg1Of	NFAT5,be
R1220	T1641	T1649	arg2Of	NFAT5,activated
R1221	T1645	T1642	arg2Of	shown,has
R1222	T1645	T1643	arg1Of	shown,previously
R1223	T1645	T1644	arg2Of	shown,been
R1224	T1649	T1645	arg3Of	activated,shown
R1225	T1649	T1646	arg1Of	activated,to
R1226	T1649	T1647	arg2Of	activated,be
R1227	T1649	T1648	arg1Of	activated,transcriptionally
R1228	T1654	T1649	arg1Of	p38,activated
R1229	T1654	T1650	arg2Of	p38,by
R1230	T1654	T1651	arg1Of	p38,the
R1231	T1654	T1652	arg1Of	p38,MAP
R1232	T1654	T1653	arg1Of	p38,kinase
R1233	T1654	T1655	arg1Of	p38,","
R1234	T1654	T1656	arg1Of	p38,which
R1235	T1654	T1657	arg1Of	p38,is
R1236	T1654	T1658	arg1Of	p38,downstream
R1237	T1657	T1662	arg1Of	is,","
R1238	T1657	T1663	arg1Of	is,[
R1239	T1658	T1657	arg2Of	downstream,is
R1240	T1658	T1659	arg1Of	downstream,of
R1241	T1661	T1659	arg2Of	signaling,of
R1242	T1661	T1660	arg1Of	signaling,MyD88
R1243	T1664	T1663	arg2Of	32,[
R1244	T1665	T1663	arg3Of	],[
R1245	T1667	T1668	arg1Of	we,speculated
R1246	T1668	T1657	arg3Of	speculated,is
R1247	T1668	T1666	arg1Of	speculated,","
R1248	T1670	T1671	arg1Of	NFAT5,may
R1249	T1670	T1673	arg1Of	NFAT5,be
R1250	T1670	T1674	arg2Of	NFAT5,involved
R1251	T1674	T1668	arg2Of	involved,speculated
R1252	T1674	T1669	arg1Of	involved,that
R1253	T1674	T1671	arg2Of	involved,may
R1254	T1674	T1672	arg1Of	involved,also
R1255	T1674	T1673	arg2Of	involved,be
R1256	T1674	T1675	arg1Of	involved,in
R1257	T1674	T1681	arg1Of	involved,via
R1258	T1677	T1675	arg2Of	activation,in
R1259	T1677	T1676	arg1Of	activation,MTb-induced
R1260	T1677	T1678	arg1Of	activation,of
R1261	T1680	T1678	arg2Of	replication,of
R1262	T1680	T1679	arg1Of	replication,HIV-1
R1263	T1684	T1681	arg2Of	pathway,via
R1264	T1684	T1682	arg1Of	pathway,a
R1265	T1684	T1683	arg1Of	pathway,TLR-mediated
R1266	T1684	T1685	arg1Of	pathway,in
R1267	T1686	T1687	arg1Of	monocytes,and
R1268	T1687	T1685	arg2Of	and,in
R1269	T1691	T1687	arg2Of	cells,and
R1270	T1691	T1688	arg1Of	cells,peripheral
R1271	T1691	T1689	arg1Of	cells,blood
R1272	T1691	T1690	arg1Of	cells,mononuclear
R1273	T1691	T1692	arg1Of	cells,(
R1274	T1693	T1692	arg2Of	PBMC,(
R1275	T1694	T1692	arg3Of	),(
R1276	T1697	T1698	arg1Of	we,show
R1277	T1698	T1695	arg1Of	show,Here
R1278	T1698	T1696	arg1Of	show,","
R1279	T1700	T1701	arg1Of	NFAT5,and
R1280	T1701	T1706	arg1Of	and,are
R1281	T1701	T1707	arg1Of	and,of
R1282	T1705	T1701	arg2Of	site,and
R1283	T1705	T1702	arg1Of	site,its
R1284	T1705	T1703	arg1Of	site,cognate
R1285	T1705	T1704	arg1Of	site,binding
R1286	T1706	T1698	arg2Of	are,show
R1287	T1706	T1699	arg1Of	are,that
R1288	T1707	T1706	arg2Of	of,are
R1289	T1709	T1707	arg2Of	importance,of
R1290	T1709	T1708	arg1Of	importance,crucial
R1291	T1709	T1710	arg1Of	importance,for
R1292	T1713	T1710	arg2Of	stimulation,for
R1293	T1713	T1711	arg1Of	stimulation,efficient
R1294	T1713	T1712	arg1Of	stimulation,MTb-induced
R1295	T1713	T1714	arg1Of	stimulation,of
R1296	T1716	T1714	arg2Of	replication,of
R1297	T1716	T1715	arg1Of	replication,HIV-1
R1298	T1716	T1717	arg1Of	replication,in
R1299	T1719	T1720	arg1Of	MDM,and
R1300	T1720	T1717	arg2Of	and,in
R1301	T1720	T1718	arg1Of	and,human
R1302	T1721	T1720	arg2Of	PBMC,and
R1303	T1724	T1725	arg1Of	we,demonstrate
R1304	T1725	T1722	arg1Of	demonstrate,Moreover
R1305	T1725	T1723	arg1Of	demonstrate,","
R1306	T1728	T1727	arg1Of	infection,MTb
R1307	T1728	T1729	arg1Of	infection,increases
R1308	T1729	T1725	arg2Of	increases,demonstrate
R1309	T1729	T1726	arg1Of	increases,that
R1310	T1729	T1736	arg1Of	increases,in
R1311	T1732	T1729	arg2Of	expression,increases
R1312	T1732	T1730	arg1Of	expression,NFAT5
R1313	T1732	T1731	arg1Of	expression,gene
R1314	T1732	T1733	arg1Of	expression,in
R1315	T1735	T1733	arg2Of	monocytes,in
R1316	T1735	T1734	arg1Of	monocytes,human
R1317	T1739	T1736	arg2Of	manner,in
R1318	T1739	T1737	arg1Of	manner,a
R1319	T1739	T1738	arg1Of	manner,MyD88-dependent
R1320	T1743	T1742	arg1Of	results,these
R1321	T1743	T1744	arg1Of	results,expand
R1322	T1743	T1759	arg1Of	results,demonstrate
R1323	T1744	T1758	arg1Of	expand,and
R1324	T1747	T1744	arg2Of	stimuli,expand
R1325	T1747	T1745	arg1Of	stimuli,the
R1326	T1747	T1746	arg1Of	stimuli,known
R1327	T1747	T1748	arg1Of	stimuli,of
R1328	T1750	T1748	arg2Of	expression,of
R1329	T1750	T1749	arg1Of	expression,NFAT5
R1330	T1750	T1751	arg1Of	expression,to
R1331	T1756	T1751	arg2Of	response,to
R1332	T1756	T1752	arg1Of	response,the
R1333	T1756	T1753	arg1Of	response,PRR-mediated
R1334	T1756	T1754	arg1Of	response,innate
R1335	T1756	T1755	arg1Of	response,immune
R1336	T1758	T1740	arg1Of	and,Thus
R1337	T1758	T1741	arg1Of	and,","
R1338	T1758	T1757	arg1Of	and,","
R1339	T1759	T1758	arg2Of	demonstrate,and
R1340	T1761	T1762	arg1Of	NFAT5,is
R1341	T1762	T1759	arg2Of	is,demonstrate
R1342	T1762	T1760	arg1Of	is,that
R1343	T1765	T1762	arg2Of	modulator,is
R1344	T1765	T1763	arg1Of	modulator,a
R1345	T1765	T1764	arg1Of	modulator,critical
R1346	T1765	T1766	arg1Of	modulator,of
R1347	T1768	T1766	arg2Of	enhancement,of
R1348	T1768	T1767	arg1Of	enhancement,MTb-induced
R1349	T1768	T1769	arg1Of	enhancement,of
R1350	T1771	T1769	arg2Of	replication,of
R1351	T1771	T1770	arg1Of	replication,HIV-1
R2162	T2815	T2814	arg1Of	statement,Ethics
R2163	T2818	T2816	arg2Of	studies,In
R2164	T2818	T2817	arg1Of	studies,our
R2165	T2819	T2820	arg1Of	we,used
R2166	T2820	T2816	arg1Of	used,In
R2167	T2825	T2820	arg2Of	cells,used
R2168	T2825	T2821	arg1Of	cells,unidentified
R2169	T2825	T2822	arg1Of	cells,human
R2170	T2825	T2823	arg2Of	cells,discarded
R2171	T2825	T2824	arg1Of	cells,blood
R2172	T2825	T2826	arg1Of	cells,(
R2173	T2825	T2834	arg1Of	cells,","
R2174	T2825	T2835	arg1Of	cells,which
R2175	T2825	T2837	arg2Of	cells,obtained
R2176	T2830	T2826	arg2Of	cells,(
R2177	T2830	T2827	arg1Of	cells,peripheral
R2178	T2830	T2828	arg1Of	cells,blood
R2179	T2830	T2829	arg1Of	cells,mononuclear
R2180	T2830	T2831	arg1Of	cells,","
R2181	T2832	T2831	arg2Of	PBMC,","
R2182	T2833	T2826	arg3Of	),(
R2183	T2836	T2837	arg1Of	we,obtained
R2184	T2837	T2838	arg1Of	obtained,from
R2185	T2841	T2839	arg1Of	Bank,the
R2186	T2841	T2840	arg1Of	Bank,Blood
R2187	T2841	T2842	arg1Of	Bank,of
R2188	T2841	T2844	arg2Of	Bank,'s
R2189	T2843	T2842	arg2Of	Children,of
R2190	T2845	T2838	arg2Of	Hospital,from
R2191	T2845	T2844	arg1Of	Hospital,'s
R2192	T2845	T2846	arg1Of	Hospital,in
R2193	T2847	T2846	arg2Of	Boston,in
R2230	T2930	T2929	arg1Of	culture,Cell
R2231	T2931	T2932	arg1Of	PBMC,from
R2232	T2931	T2936	arg1Of	PBMC,were
R2233	T2931	T2937	arg2Of	PBMC,isolated
R2234	T2931	T2952	arg1Of	PBMC,were
R2235	T2931	T2953	arg2Of	PBMC,cultured
R2236	T2935	T2932	arg2Of	donors,from
R2237	T2935	T2933	arg1Of	donors,normal
R2238	T2935	T2934	arg1Of	donors,unidentified
R2239	T2937	T2936	arg2Of	isolated,were
R2240	T2937	T2938	arg1Of	isolated,by
R2241	T2937	T2951	arg1Of	isolated,and
R2242	T2942	T2940	arg2Of	Corporation,(
R2243	T2942	T2941	arg1Of	Corporation,Pharmacia
R2244	T2942	T2943	arg1Of	Corporation,","
R2245	T2944	T2943	arg2Of	Peapack,","
R2246	T2944	T2945	arg1Of	Peapack,","
R2247	T2944	T2946	arg1Of	Peapack,NJ
R2248	T2947	T2940	arg3Of	),(
R2249	T2950	T2938	arg2Of	centrifugation,by
R2250	T2950	T2939	arg1Of	centrifugation,Ficoll-Hypaque
R2251	T2950	T2940	arg1Of	centrifugation,(
R2252	T2950	T2948	arg1Of	centrifugation,density
R2253	T2950	T2949	arg1Of	centrifugation,gradient
R2254	T2953	T2951	arg2Of	cultured,and
R2255	T2953	T2952	arg2Of	cultured,were
R2256	T2953	T2954	arg1Of	cultured,in
R2257	T2957	T2954	arg2Of	medium,in
R2258	T2957	T2955	arg1Of	medium,RPMI
R2259	T2957	T2956	arg1Of	medium,1640
R2260	T2957	T2958	arg1Of	medium,with
R2261	T2959	T2960	arg1Of	2,mM
R2262	T2961	T2958	arg2Of	L-glutamine,with
R2263	T2961	T2959	arg1Of	L-glutamine,2
R2264	T2961	T2962	arg1Of	L-glutamine,(
R2265	T2961	T2971	arg2Of	L-glutamine,supplemented
R2266	T2963	T2964	arg1Of	BioWhittaker,","
R2267	T2964	T2966	arg1Of	",",","
R2268	T2965	T2964	arg2Of	Inc.,","
R2269	T2966	T2962	arg2Of	",",(
R2270	T2966	T2968	arg1Of	",",","
R2271	T2966	T2969	arg1Of	",",MD
R2272	T2967	T2966	arg2Of	Walkersville,","
R2273	T2970	T2962	arg3Of	),(
R2274	T2971	T2972	arg1Of	supplemented,with
R2275	T2973	T2974	arg1Of	10,%
R2276	T2978	T2972	arg2Of	serum,with
R2277	T2978	T2973	arg1Of	serum,10
R2278	T2978	T2975	arg1Of	serum,heat-inactivated
R2279	T2978	T2976	arg1Of	serum,fetal
R2280	T2978	T2977	arg1Of	serum,calf
R2281	T2978	T2979	arg1Of	serum,(
R2282	T2978	T2982	arg1Of	serum,(
R2283	T2980	T2979	arg2Of	FCS,(
R2284	T2981	T2979	arg3Of	),(
R2285	T2984	T2982	arg2Of	Bio-Products,(
R2286	T2984	T2983	arg1Of	Bio-Products,Gemini
R2287	T2984	T2985	arg1Of	Bio-Products,","
R2288	T2986	T2985	arg2Of	www.gembio.com,","
R2289	T2987	T2982	arg3Of	),(
R2290	T2989	T2988	arg1Of	monocytes,Human
R2291	T2989	T2990	arg1Of	monocytes,were
R2292	T2989	T2991	arg2Of	monocytes,isolated
R2293	T2989	T3014	arg1Of	monocytes,were
R2294	T2989	T3015	arg2Of	monocytes,cultured
R2295	T2991	T2990	arg2Of	isolated,were
R2296	T2991	T2992	arg1Of	isolated,from
R2297	T2991	T2995	arg1Of	isolated,by
R2298	T2991	T2998	arg1Of	isolated,with
R2299	T2991	T3007	arg1Of	isolated,as
R2300	T2991	T3013	arg1Of	isolated,and
R2301	T2994	T2992	arg2Of	preparations,from
R2302	T2994	T2993	arg1Of	preparations,PBMC
R2303	T2997	T2995	arg2Of	selection,by
R2304	T2997	T2996	arg1Of	selection,positive
R2305	T3000	T2998	arg2Of	microbeads,with
R2306	T3000	T2999	arg1Of	microbeads,CD14
R2307	T3000	T3001	arg1Of	microbeads,from
R2308	T3003	T3001	arg2Of	Biotec,from
R2309	T3003	T3002	arg1Of	Biotec,Miltenyi
R2310	T3003	T3004	arg1Of	Biotec,(
R2311	T3005	T3004	arg2Of	www.miltenyibiotec.com,(
R2312	T3006	T3004	arg3Of	),(
R2313	T3008	T3007	arg2Of	described,as
R2314	T3011	T3008	arg1Of	manufacturer,described
R2315	T3011	T3009	arg2Of	manufacturer,by
R2316	T3011	T3010	arg1Of	manufacturer,the
R2317	T3013	T3012	arg1Of	and,","
R2318	T3015	T3013	arg2Of	cultured,and
R2319	T3015	T3014	arg2Of	cultured,were
R2320	T3015	T3016	arg1Of	cultured,at
R2321	T3015	T3021	arg1Of	cultured,in
R2322	T3015	T3024	arg1Of	cultured,in
R2323	T3018	T3016	arg2Of	cells,at
R2324	T3018	T3017	arg1Of	cells,1×106
R2325	T3018	T3019	arg1Of	cells,per
R2326	T3020	T3019	arg2Of	well,per
R2327	T3023	T3021	arg2Of	plates,in
R2328	T3023	T3022	arg1Of	plates,6-well
R2329	T3026	T3024	arg2Of	medium,in
R2330	T3026	T3025	arg1Of	medium,Macrophage-SFM
R2331	T3026	T3027	arg1Of	medium,(
R2332	T3026	T3032	arg2Of	medium,supplemented
R2333	T3028	T3027	arg2Of	Gibco,(
R2334	T3028	T3029	arg1Of	Gibco,","
R2335	T3028	T3030	arg1Of	Gibco,www.invitrogen.com
R2336	T3031	T3027	arg3Of	),(
R2337	T3032	T3033	arg1Of	supplemented,with
R2338	T3032	T3053	arg1Of	supplemented,(
R2339	T3038	T3034	arg1Of	MCSF,15
R2340	T3038	T3035	arg1Of	MCSF,ng/ml
R2341	T3038	T3036	arg1Of	MCSF,recombinant
R2342	T3038	T3037	arg1Of	MCSF,human
R2343	T3038	T3039	arg1Of	MCSF,(
R2344	T3038	T3046	arg1Of	MCSF,and
R2345	T3042	T3039	arg2Of	D,(
R2346	T3042	T3040	arg1Of	D,R
R2347	T3042	T3041	arg1Of	D,&
R2348	T3042	T3043	arg1Of	D,","
R2349	T3044	T3043	arg2Of	www.rndsystems.com,","
R2350	T3045	T3039	arg3Of	),(
R2351	T3046	T3033	arg2Of	and,with
R2352	T3047	T3048	arg1Of	5,%
R2353	T3052	T3046	arg2Of	serum,and
R2354	T3052	T3047	arg1Of	serum,5
R2355	T3052	T3049	arg1Of	serum,heat-inactivated
R2356	T3052	T3050	arg1Of	serum,human
R2357	T3052	T3051	arg1Of	serum,AB
R2358	T3054	T3053	arg2Of	Nabi,(
R2359	T3054	T3055	arg1Of	Nabi,","
R2360	T3057	T3055	arg2Of	Raton,","
R2361	T3057	T3056	arg1Of	Raton,Boca
R2362	T3057	T3058	arg1Of	Raton,","
R2363	T3059	T3058	arg2Of	FL,","
R2364	T3060	T3053	arg3Of	),(
R2365	T3063	T3061	arg1Of	cultures,The
R2366	T3063	T3062	arg1Of	cultures,cell
R2367	T3063	T3064	arg1Of	cultures,were
R2368	T3063	T3065	arg2Of	cultures,incubated
R2369	T3065	T3064	arg2Of	incubated,were
R2370	T3065	T3066	arg1Of	incubated,at
R2371	T3065	T3072	arg1Of	incubated,for
R2372	T3067	T3068	arg1Of	37°C,and
R2373	T3070	T3068	arg2Of	%,and
R2374	T3070	T3069	arg1Of	%,5
R2375	T3071	T3066	arg2Of	CO2,at
R2376	T3071	T3067	arg1Of	CO2,37°C
R2377	T3071	T3070	arg1Of	CO2,%
R2378	T3074	T3072	arg2Of	days,for
R2379	T3074	T3073	arg1Of	days,5
R2380	T3074	T3075	arg1Of	days,","
R2381	T3074	T3076	arg2Of	days,after
R2382	T3074	T3077	arg1Of	days,which
R2383	T3078	T3079	arg1Of	supernatant,was
R2384	T3078	T3080	arg2Of	supernatant,replaced
R2385	T3080	T3076	arg1Of	replaced,after
R2386	T3080	T3079	arg2Of	replaced,was
R2387	T3080	T3081	arg1Of	replaced,with
R2388	T3083	T3081	arg2Of	medium,with
R2389	T3083	T3082	arg1Of	medium,fresh
R2390	T3083	T3084	arg1Of	medium,lacking
R2391	T3084	T3086	arg1Of	lacking,before
R2392	T3085	T3084	arg2Of	MCSF,lacking
R2393	T3087	T3086	arg2Of	manipulation,before
R2394	T3088	T3089	arg1Of	More,than
R2395	T3090	T3089	arg2Of	95,than
R2396	T3091	T3088	arg1Of	%,More
R2397	T3091	T3092	arg1Of	%,of
R2398	T3091	T3100	arg1Of	%,were
R2399	T3095	T3092	arg2Of	cells,of
R2400	T3095	T3093	arg1Of	cells,the
R2401	T3095	T3094	arg1Of	cells,adherent
R2402	T3095	T3096	arg2Of	cells,obtained
R2403	T3096	T3097	arg1Of	obtained,with
R2404	T3099	T3097	arg2Of	technique,with
R2405	T3099	T3098	arg1Of	technique,this
R2406	T3100	T3103	arg1Of	were,as
R2407	T3102	T3100	arg2Of	macrophages,were
R2408	T3102	T3101	arg1Of	macrophages,CD14+
R2409	T3104	T3103	arg2Of	verified,as
R2410	T3107	T3104	arg1Of	cytometry,verified
R2411	T3107	T3105	arg2Of	cytometry,by
R2412	T3107	T3106	arg1Of	cytometry,flow
R2413	T3109	T3108	arg1Of	cells,THP-1
R2414	T3109	T3110	arg1Of	cells,were
R2415	T3109	T3111	arg2Of	cells,obtained
R2416	T3109	T3118	arg2Of	cells,cultured
R2417	T3111	T3112	arg1Of	obtained,from
R2418	T3111	T3117	arg1Of	obtained,and
R2419	T3113	T3112	arg2Of	ATCC,from
R2420	T3113	T3114	arg1Of	ATCC,(
R2421	T3115	T3114	arg2Of	www.atcc.org,(
R2422	T3116	T3114	arg3Of	),(
R2423	T3117	T3110	arg2Of	and,were
R2424	T3117	T3128	arg1Of	and,(
R2425	T3118	T3117	arg2Of	cultured,and
R2426	T3118	T3119	arg1Of	cultured,in
R2427	T3122	T3119	arg2Of	medium,in
R2428	T3122	T3120	arg1Of	medium,RPMI
R2429	T3122	T3121	arg1Of	medium,1640
R2430	T3122	T3123	arg2Of	medium,supplemented
R2431	T3123	T3124	arg1Of	supplemented,with
R2432	T3125	T3126	arg1Of	10,%
R2433	T3127	T3124	arg2Of	FCS,with
R2434	T3127	T3125	arg1Of	FCS,10
R2435	T3129	T3128	arg2Of	BioWhittaker,(
R2436	T3129	T3130	arg1Of	BioWhittaker,","
R2437	T3129	T3131	arg1Of	BioWhittaker,www.lonzabio.com
R2438	T3132	T3128	arg3Of	),(
R2439	T3134	T3133	arg1Of	cells,293T
R2440	T3134	T3135	arg1Of	cells,were
R2441	T3134	T3136	arg2Of	cells,obtained
R2442	T3134	T3143	arg1Of	cells,were
R2443	T3134	T3144	arg2Of	cells,maintained
R2444	T3136	T3135	arg2Of	obtained,were
R2445	T3136	T3137	arg1Of	obtained,from
R2446	T3136	T3142	arg1Of	obtained,and
R2447	T3138	T3137	arg2Of	ATCC,from
R2448	T3138	T3139	arg1Of	ATCC,(
R2449	T3140	T3139	arg2Of	www.atcc.org,(
R2450	T3141	T3139	arg3Of	),(
R2451	T3144	T3142	arg2Of	maintained,and
R2452	T3144	T3143	arg2Of	maintained,were
R2453	T3144	T3145	arg1Of	maintained,in
R2454	T3146	T3147	arg2Of	Dulbecco,'s
R2455	T3149	T3147	arg1Of	Eagle,'s
R2456	T3149	T3148	arg1Of	Eagle,Modified
R2457	T3149	T3150	arg2Of	Eagle,'s
R2458	T3151	T3145	arg2Of	medium,in
R2459	T3151	T3150	arg1Of	medium,'s
R2460	T3151	T3152	arg1Of	medium,(
R2461	T3151	T3155	arg1Of	medium,(
R2462	T3151	T3160	arg2Of	medium,supplemented
R2463	T3153	T3152	arg2Of	DMEM,(
R2464	T3154	T3152	arg3Of	),(
R2465	T3156	T3155	arg2Of	Gibco,(
R2466	T3156	T3157	arg1Of	Gibco,","
R2467	T3156	T3158	arg1Of	Gibco,www.invitrogen.com
R2468	T3159	T3155	arg3Of	),(
R2469	T3160	T3161	arg1Of	supplemented,with
R2470	T3162	T3163	arg1Of	10,%
R2471	T3164	T3161	arg2Of	FCS,with
R2472	T3164	T3162	arg1Of	FCS,10
R2721	T3471	T3472	arg1Of	HIV-1Lai,","
R2722	T3472	T3474	arg1Of	",",","
R2723	T3473	T3472	arg2Of	HIV-193TH64,","
R2724	T3474	T3476	arg1Of	",",","
R2725	T3475	T3474	arg2Of	HIV-192TH51,","
R2726	T3476	T3478	arg1Of	",",","
R2727	T3477	T3476	arg2Of	HIV-192TH53,","
R2728	T3478	T3481	arg1Of	",",and
R2729	T3479	T3478	arg2Of	HIV-198CH01,","
R2730	T3481	T3480	arg1Of	and,","
R2731	T3481	T3483	arg1Of	and,were
R2732	T3481	T3484	arg2Of	and,obtained
R2733	T3482	T3481	arg2Of	HIV-198IN22,and
R2734	T3484	T3469	arg1Of	obtained,HIV-1Bal
R2735	T3484	T3470	arg1Of	obtained,","
R2736	T3484	T3483	arg2Of	obtained,were
R2737	T3484	T3485	arg1Of	obtained,from
R2738	T3484	T3503	arg1Of	obtained,","
R2739	T3488	T3487	arg1Of	Facility,Centralized
R2740	T3488	T3489	arg1Of	Facility,for
R2741	T3488	T3492	arg1Of	Facility,","
R2742	T3491	T3489	arg2Of	Reagents,for
R2743	T3491	T3490	arg1Of	Reagents,AIDS
R2744	T3492	T3498	arg1Of	",",and
R2745	T3494	T3492	arg2Of	Institute,","
R2746	T3494	T3493	arg1Of	Institute,National
R2747	T3494	T3495	arg1Of	Institute,for
R2748	T3497	T3495	arg2Of	Standard,for
R2749	T3497	T3496	arg1Of	Standard,Biological
R2750	T3498	T3485	arg2Of	and,from
R2751	T3498	T3486	arg1Of	and,The
R2752	T3499	T3498	arg2Of	Control,and
R2753	T3499	T3500	arg1Of	Control,(
R2754	T3501	T3500	arg2Of	NIBSC,(
R2755	T3502	T3500	arg3Of	),(
R2756	T3505	T3504	arg1Of	Kingdom.,United
R2757	T3506	T3505	arg1Of	HIV-1KR25,Kingdom.
R2758	T3506	T3507	arg1Of	HIV-1KR25,was
R2759	T3506	T3508	arg2Of	HIV-1KR25,isolated
R2760	T3508	T3503	arg2Of	isolated,","
R2761	T3508	T3507	arg2Of	isolated,was
R2762	T3508	T3509	arg1Of	isolated,in
R2763	T3511	T3509	arg2Of	laboratory,in
R2764	T3511	T3510	arg1Of	laboratory,our
R2765	T3511	T3512	arg1Of	laboratory,as
R2766	T3513	T3512	arg2Of	described,as
R2767	T3513	T3514	arg1Of	described,before
R2768	T3516	T3514	arg2Of	33,before
R2769	T3516	T3515	arg2Of	33,[
R2770	T3517	T3515	arg3Of	],[
R2825	T3608	T3609	arg1Of	construction,and
R2826	T3610	T3609	arg2Of	reporter,and
R2827	T3611	T3606	arg1Of	assay,LTR
R2828	T3611	T3607	arg1Of	assay,plasmid
R2829	T3611	T3608	arg1Of	assay,construction
R2830	T3611	T3610	arg1Of	assay,reporter
R2831	T3614	T3612	arg1Of	plasmids,LTR
R2832	T3614	T3613	arg1Of	plasmids,reporter
R2833	T3614	T3615	arg1Of	plasmids,were
R2834	T3614	T3616	arg2Of	plasmids,constructed
R2835	T3616	T3615	arg2Of	constructed,were
R2836	T3616	T3617	arg1Of	constructed,by
R2837	T3618	T3617	arg2Of	inserting,by
R2838	T3618	T3673	modOf	inserting,using
R2839	T3619	T3620	arg1Of	nucleotides,−208
R2840	T3619	T3637	arg1Of	nucleotides,","
R2841	T3620	T3621	arg1Of	−208,to
R2842	T3622	T3621	arg2Of	+64,to
R2843	T3622	T3623	arg1Of	+64,relative
R2844	T3623	T3624	arg1Of	relative,to
R2845	T3628	T3624	arg2Of	site,to
R2846	T3628	T3625	arg1Of	site,the
R2847	T3628	T3626	arg1Of	site,transcriptional
R2848	T3628	T3627	arg1Of	site,initiation
R2849	T3628	T3629	arg1Of	site,of
R2850	T3628	T3631	arg1Of	site,","
R2851	T3630	T3629	arg2Of	HIV-1Lai,of
R2852	T3632	T3631	arg2Of	HIV-1Bal,","
R2853	T3632	T3633	arg1Of	HIV-1Bal,(
R2854	T3635	T3633	arg2Of	subtype,(
R2855	T3635	T3634	arg1Of	subtype,B
R2856	T3636	T3633	arg3Of	),(
R2857	T3637	T3618	arg2Of	",",inserting
R2858	T3637	T3662	arg1Of	",",into
R2859	T3637	T3667	arg1Of	",",(
R2860	T3638	T3639	arg1Of	HIV-198IN17,","
R2861	T3639	T3641	arg1Of	",",","
R2862	T3640	T3639	arg2Of	HIV-198IN22,","
R2863	T3641	T3643	arg1Of	",",","
R2864	T3642	T3641	arg2Of	HIV-198CH01,","
R2865	T3643	T3649	arg1Of	",",","
R2866	T3644	T3643	arg2Of	HIV-1CM9,","
R2867	T3644	T3645	arg1Of	HIV-1CM9,(
R2868	T3647	T3645	arg2Of	subtype,(
R2869	T3647	T3646	arg1Of	subtype,C
R2870	T3648	T3645	arg3Of	),(
R2871	T3649	T3637	arg2Of	",",","
R2872	T3650	T3651	arg1Of	HIV-193TH64,","
R2873	T3651	T3653	arg1Of	",",","
R2874	T3652	T3651	arg2Of	HIV-192TH53,","
R2875	T3653	T3656	arg1Of	",",and
R2876	T3654	T3653	arg2Of	HIV-192TH51,","
R2877	T3656	T3649	arg2Of	and,","
R2878	T3656	T3655	arg1Of	and,","
R2879	T3657	T3656	arg2Of	HIV-1KR25,and
R2880	T3657	T3658	arg1Of	HIV-1KR25,(
R2881	T3660	T3658	arg2Of	subtype,(
R2882	T3660	T3659	arg1Of	subtype,E
R2883	T3661	T3658	arg3Of	),(
R2884	T3666	T3662	arg2Of	pGL3,into
R2885	T3666	T3663	arg1Of	pGL3,the
R2886	T3666	T3664	arg1Of	pGL3,reporter
R2887	T3666	T3665	arg1Of	pGL3,vector
R2888	T3669	T3667	arg2Of	BioSciences,(
R2889	T3669	T3668	arg1Of	BioSciences,Promega
R2890	T3669	T3670	arg1Of	BioSciences,","
R2891	T3671	T3670	arg2Of	www.promega.com,","
R2892	T3672	T3667	arg3Of	),(
R2893	T3674	T3675	arg1Of	Xho,I
R2894	T3674	T3676	arg1Of	Xho,and
R2895	T3676	T3673	arg2Of	and,using
R2896	T3681	T3676	arg2Of	sites,and
R2897	T3681	T3677	arg1Of	sites,Hind
R2898	T3681	T3678	arg1Of	sites,III
R2899	T3681	T3679	arg1Of	sites,restriction
R2900	T3681	T3680	arg1Of	sites,enzyme
R2901	T3682	T3683	arg1Of	Sequences,were
R2902	T3682	T3684	arg2Of	Sequences,aligned
R2903	T3682	T3686	arg2Of	Sequences,analyzed
R2904	T3684	T3685	arg1Of	aligned,and
R2905	T3685	T3683	arg2Of	and,were
R2906	T3686	T3685	arg2Of	analyzed,and
R2907	T3686	T3687	arg1Of	analyzed,with
R2908	T3689	T3687	arg2Of	W,with
R2909	T3689	T3688	arg1Of	W,CLUSTAL
R2910	T3689	T3690	arg1Of	W,(
R2911	T3691	T3690	arg2Of	www.ebi.ac.uk/clustalw/,(
R2912	T3692	T3690	arg3Of	),(
R2913	T3699	T3698	arg2Of	N5-Mut,(
R2914	T3700	T3698	arg3Of	),(
R2915	T3702	T3693	arg1Of	plasmid,The
R2916	T3702	T3694	arg1Of	plasmid,HIV-1Lai
R2917	T3702	T3695	arg1Of	plasmid,NFAT5
R2918	T3702	T3696	arg1Of	plasmid,binding
R2919	T3702	T3697	arg1Of	plasmid,site-mutant
R2920	T3702	T3698	arg1Of	plasmid,(
R2921	T3702	T3701	arg1Of	plasmid,reporter
R2922	T3702	T3703	arg1Of	plasmid,was
R2923	T3702	T3704	arg2Of	plasmid,created
R2924	T3704	T3703	arg2Of	created,was
R2925	T3709	T3704	arg1Of	methods,created
R2926	T3709	T3705	arg2Of	methods,by
R2927	T3709	T3706	arg1Of	methods,standard
R2928	T3709	T3707	arg1Of	methods,PCR-based
R2929	T3709	T3708	arg1Of	methods,mutagenesis
R2930	T3709	T3710	arg1Of	methods,[
R2931	T3711	T3710	arg2Of	34,[
R2932	T3712	T3710	arg3Of	],[
R2933	T3714	T3713	arg1Of	cells,THP-1
R2934	T3714	T3715	arg1Of	cells,(
R2935	T3714	T3718	arg1Of	cells,were
R2936	T3714	T3719	arg2Of	cells,transfected
R2937	T3714	T3744	arg1Of	cells,using
R2938	T3716	T3715	arg2Of	0.8×106/ml,(
R2939	T3717	T3715	arg3Of	),(
R2940	T3719	T3718	arg2Of	transfected,were
R2941	T3719	T3720	arg1Of	transfected,with
R2942	T3719	T3744	modOf	transfected,using
R2943	T3721	T3722	arg1Of	0.3,µg/ml
R2944	T3723	T3721	arg1Of	LTR,0.3
R2945	T3723	T3724	arg1Of	LTR,wild-type
R2946	T3723	T3725	arg1Of	LTR,(
R2947	T3723	T3728	arg1Of	LTR,or
R2948	T3726	T3725	arg2Of	WT,(
R2949	T3727	T3725	arg3Of	),(
R2950	T3728	T3720	arg2Of	or,with
R2951	T3728	T3732	arg1Of	or,in
R2952	T3731	T3728	arg2Of	plasmids,or
R2953	T3731	T3729	arg2Of	plasmids,mutated
R2954	T3731	T3730	arg1Of	plasmids,reporter
R2955	T3733	T3732	arg2Of	combination,in
R2956	T3733	T3734	arg1Of	combination,with
R2957	T3738	T3735	arg1Of	luciferase,0.03
R2958	T3738	T3736	arg1Of	luciferase,µg/ml
R2959	T3738	T3737	arg1Of	luciferase,Renilla
R2960	T3738	T3739	arg1Of	luciferase,(
R2961	T3740	T3739	arg2Of	pRL-TK,(
R2962	T3741	T3739	arg3Of	),(
R2963	T3743	T3734	arg2Of	vector,with
R2964	T3743	T3738	arg1Of	vector,luciferase
R2965	T3743	T3742	arg1Of	vector,control
R2966	T3747	T3744	arg2Of	reagent,using
R2967	T3747	T3745	arg1Of	reagent,Effectene
R2968	T3747	T3746	arg1Of	reagent,transfection
R2969	T3747	T3748	arg1Of	reagent,(
R2970	T3749	T3748	arg2Of	Qiagen,(
R2971	T3749	T3750	arg1Of	Qiagen,;
R2972	T3751	T3750	arg2Of	www.qiagen.com,;
R2973	T3752	T3748	arg3Of	),(
R2974	T3753	T3754	arg1Of	Cells,were
R2975	T3753	T3755	arg2Of	Cells,incubated
R2976	T3755	T3754	arg2Of	incubated,were
R2977	T3755	T3756	arg1Of	incubated,at
R2978	T3755	T3758	arg1Of	incubated,for
R2979	T3757	T3756	arg2Of	37°C,at
R2980	T3760	T3758	arg2Of	hours,for
R2981	T3760	T3759	arg1Of	hours,16
R2982	T3760	T3761	arg2Of	hours,after
R2983	T3760	T3762	arg1Of	hours,which
R2984	T3763	T3764	arg1Of	they,were
R2985	T3763	T3765	arg2Of	they,stimulated
R2986	T3763	T3773	arg1Of	they,left
R2987	T3765	T3764	arg2Of	stimulated,were
R2988	T3765	T3766	arg1Of	stimulated,with
R2989	T3765	T3772	arg1Of	stimulated,or
R2990	T3771	T3766	arg2Of	lysate,with
R2991	T3771	T3767	arg1Of	lysate,10
R2992	T3771	T3768	arg1Of	lysate,µg/ml
R2993	T3771	T3769	arg1Of	lysate,MTb
R2994	T3771	T3770	arg1Of	lysate,CDC1551
R2995	T3772	T3761	arg1Of	or,after
R2996	T3773	T3772	arg2Of	left,or
R2997	T3773	T3775	arg1Of	left,for
R2998	T3774	T3773	arg2Of	unstimulated,left
R2999	T3777	T3775	arg2Of	hours,for
R3000	T3777	T3776	arg1Of	hours,8
R3001	T3780	T3778	arg1Of	expression,Reporter
R3002	T3780	T3779	arg1Of	expression,gene
R3003	T3780	T3781	arg1Of	expression,was
R3004	T3780	T3782	arg2Of	expression,quantitated
R3005	T3782	T3781	arg2Of	quantitated,was
R3006	T3782	T3787	arg1Of	quantitated,according
R3007	T3786	T3782	arg1Of	assay,quantitated
R3008	T3786	T3783	arg2Of	assay,by
R3009	T3786	T3784	arg1Of	assay,dual-luciferase
R3010	T3786	T3785	arg1Of	assay,reporter
R3011	T3788	T3787	arg2Of	to,according
R3012	T3790	T3789	arg1Of	manufacturer,the
R3013	T3790	T3791	arg2Of	manufacturer,'s
R3014	T3792	T3788	arg2Of	protocol,to
R3015	T3792	T3791	arg1Of	protocol,'s
R3016	T3792	T3793	arg1Of	protocol,(
R3017	T3794	T3793	arg2Of	Promega,(
R3018	T3794	T3795	arg1Of	Promega,;
R3019	T3796	T3795	arg2Of	www.promega.com,;
R3020	T3797	T3793	arg3Of	),(
R3240	T4109	T4106	arg1Of	footprinting,Quantitative
R3241	T4109	T4107	arg1Of	footprinting,DNase
R3242	T4109	T4108	arg1Of	footprinting,I
R3243	T4111	T4110	arg1Of	NFAT5,Recombinant
R3244	T4111	T4112	arg1Of	NFAT5,(
R3245	T4111	T4117	arg1Of	NFAT5,with
R3246	T4111	T4143	arg1Of	NFAT5,using
R3247	T4114	T4112	arg2Of	acids,(
R3248	T4114	T4113	arg1Of	acids,amino
R3249	T4114	T4115	arg1Of	acids,175–471
R3250	T4116	T4112	arg3Of	),(
R3251	T4120	T4117	arg2Of	6×,with
R3252	T4120	T4118	arg1Of	6×,an
R3253	T4120	T4119	arg1Of	6×,N-terminal
R3254	T4122	T4121	arg1Of	tag,His
R3255	T4122	T4123	arg1Of	tag,was
R3256	T4122	T4124	arg2Of	tag,expressed
R3257	T4122	T4139	arg2Of	tag,purified
R3258	T4124	T4125	arg1Of	expressed,in
R3259	T4124	T4138	arg1Of	expressed,and
R3260	T4128	T4126	arg1Of	BL21,E.
R3261	T4128	T4127	arg1Of	BL21,coli
R3262	T4128	T4129	arg1Of	BL21,(
R3263	T4130	T4129	arg2Of	DE3,(
R3264	T4131	T4129	arg3Of	),(
R3265	T4132	T4125	arg2Of	cells,in
R3266	T4132	T4128	arg1Of	cells,BL21
R3267	T4132	T4133	arg1Of	cells,(
R3268	T4134	T4133	arg2Of	Stratagene,(
R3269	T4134	T4135	arg1Of	Stratagene,;
R3270	T4136	T4135	arg2Of	www.stratagene.com,;
R3271	T4137	T4133	arg3Of	),(
R3272	T4138	T4123	arg2Of	and,was
R3273	T4139	T4138	arg2Of	purified,and
R3274	T4139	T4140	arg1Of	purified,under
R3275	T4142	T4140	arg2Of	conditions,under
R3276	T4142	T4141	arg1Of	conditions,native
R3277	T4143	T4123	modOf	using,was
R3278	T4145	T4143	arg2Of	agarose,using
R3279	T4145	T4144	arg1Of	agarose,Ni-NTA
R3280	T4145	T4146	arg1Of	agarose,(
R3281	T4147	T4146	arg2Of	Qiagen,(
R3282	T4148	T4146	arg3Of	),(
R3283	T4150	T4151	arg1Of	p50,and
R3284	T4151	T4149	arg1Of	and,Recombinant
R3285	T4151	T4153	arg1Of	and,were
R3286	T4151	T4154	arg2Of	and,purchased
R3287	T4152	T4151	arg2Of	p65,and
R3288	T4154	T4153	arg2Of	purchased,were
R3289	T4154	T4155	arg1Of	purchased,(
R3290	T4157	T4155	arg2Of	Motif,(
R3291	T4157	T4156	arg1Of	Motif,Active
R3292	T4157	T4158	arg1Of	Motif,","
R3293	T4159	T4158	arg2Of	www.activemotif.com,","
R3294	T4160	T4155	arg3Of	),(
R3295	T4164	T4161	arg1Of	footprinting,Quantitative
R3296	T4164	T4162	arg1Of	footprinting,DNase
R3297	T4164	T4163	arg1Of	footprinting,I
R3298	T4164	T4165	arg1Of	footprinting,was
R3299	T4164	T4166	arg2Of	footprinting,performed
R3300	T4166	T4165	arg2Of	performed,was
R3301	T4166	T4167	arg1Of	performed,as
R3302	T4169	T4168	arg1Of	described,previously
R3303	T4171	T4167	arg2Of	31,as
R3304	T4171	T4169	arg1Of	31,described
R3305	T4171	T4170	arg2Of	31,[
R3306	T4172	T4170	arg3Of	],[
R3392	T4339	T4336	arg1Of	clones,HIV-1
R3393	T4339	T4337	arg1Of	clones,infectious
R3394	T4339	T4338	arg1Of	clones,molecular
R3395	T4341	T4340	arg1Of	plasmid,The
R3396	T4341	T4342	arg1Of	plasmid,encoding
R3397	T4341	T4350	arg1Of	plasmid,was
R3398	T4341	T4351	arg2Of	plasmid,obtained
R3399	T4347	T4342	arg2Of	clone,encoding
R3400	T4347	T4343	arg1Of	clone,the
R3401	T4347	T4344	arg1Of	clone,full-length
R3402	T4347	T4345	arg1Of	clone,infectious
R3403	T4347	T4346	arg1Of	clone,molecular
R3404	T4347	T4348	arg1Of	clone,of
R3405	T4349	T4348	arg2Of	HIV-1Lai,of
R3406	T4351	T4350	arg2Of	obtained,was
R3407	T4351	T4352	arg1Of	obtained,from
R3408	T4356	T4354	arg1Of	Reagent,NIH
R3409	T4356	T4355	arg1Of	Reagent,AIDS
R3410	T4356	T4357	arg1Of	Reagent,and
R3411	T4357	T4352	arg2Of	and,from
R3412	T4357	T4353	arg1Of	and,the
R3413	T4359	T4357	arg2Of	Program.,and
R3414	T4359	T4358	arg1Of	Program.,Reference
R3415	T4364	T4360	arg1Of	clone,The
R3416	T4364	T4361	arg1Of	clone,HIV-1Lai/Bal-Env
R3417	T4364	T4362	arg1Of	clone,infectious
R3418	T4364	T4363	arg1Of	clone,molecular
R3419	T4364	T4365	arg1Of	clone,was
R3420	T4364	T4366	arg2Of	clone,constructed
R3421	T4366	T4350	modOf	constructed,was
R3422	T4366	T4365	arg2Of	constructed,was
R3423	T4366	T4367	arg1Of	constructed,by
R3424	T4368	T4367	arg2Of	replacing,by
R3425	T4372	T4371	arg2Of	env,(
R3426	T4373	T4371	arg3Of	),(
R3427	T4376	T4368	arg2Of	acids,replacing
R3428	T4376	T4369	arg1Of	acids,the
R3429	T4376	T4370	arg1Of	acids,envelope
R3430	T4376	T4371	arg1Of	acids,(
R3431	T4376	T4374	arg1Of	acids,gp160
R3432	T4376	T4375	arg1Of	acids,amino
R3433	T4376	T4377	arg1Of	acids,103–717
R3434	T4376	T4378	arg1Of	acids,of
R3435	T4380	T4381	arg1Of	HIV-1Lai,(
R3436	T4383	T4381	arg2Of	subtype,(
R3437	T4383	T4382	arg1Of	subtype,B
R3438	T4383	T4384	arg1Of	subtype,that
R3439	T4383	T4385	arg1Of	subtype,utilizes
R3440	T4386	T4385	arg2Of	CXCR4,utilizes
R3441	T4387	T4381	arg3Of	),(
R3442	T4389	T4378	arg2Of	clone,of
R3443	T4389	T4379	arg1Of	clone,the
R3444	T4389	T4380	arg1Of	clone,HIV-1Lai
R3445	T4389	T4388	arg1Of	clone,molecular
R3446	T4389	T4390	arg1Of	clone,with
R3447	T4393	T4390	arg2Of	region,with
R3448	T4393	T4391	arg1Of	region,the
R3449	T4393	T4392	arg1Of	region,corresponding
R3450	T4393	T4394	arg1Of	region,of
R3451	T4395	T4394	arg2Of	HIV-1Bal,of
R3452	T4395	T4396	arg1Of	HIV-1Bal,(
R3453	T4398	T4396	arg2Of	subtype,(
R3454	T4398	T4397	arg1Of	subtype,B
R3455	T4398	T4399	arg1Of	subtype,that
R3456	T4398	T4400	arg1Of	subtype,utilizes
R3457	T4401	T4400	arg2Of	CCR5,utilizes
R3458	T4402	T4396	arg3Of	),(
R3459	T4406	T4403	arg1Of	virus,The
R3460	T4406	T4404	arg1Of	virus,HIV-1Lai/Bal-Env
R3461	T4406	T4405	arg1Of	virus,chimeric
R3462	T4406	T4407	arg1Of	virus,uses
R3463	T4407	T4409	arg1Of	uses,as
R3464	T4408	T4407	arg2Of	CCR5,uses
R3465	T4412	T4409	arg2Of	receptor,as
R3466	T4412	T4410	arg1Of	receptor,a
R3467	T4412	T4411	arg1Of	receptor,secondary
R3468	T4416	T4413	arg1Of	clone,The
R3469	T4416	T4414	arg1Of	clone,infectious
R3470	T4416	T4415	arg1Of	clone,molecular
R3471	T4416	T4417	arg1Of	clone,of
R3472	T4416	T4419	arg1Of	clone,was
R3473	T4416	T4420	arg2Of	clone,constructed
R3474	T4418	T4417	arg2Of	HIV-198IN22,of
R3475	T4420	T4419	arg2Of	constructed,was
R3476	T4420	T4421	modOf	constructed,using
R3477	T4422	T4421	arg2Of	DNA,using
R3478	T4422	T4423	arg2Of	DNA,extracted
R3479	T4422	T4426	arg1Of	DNA,that
R3480	T4422	T4427	arg1Of	DNA,were
R3481	T4422	T4428	arg2Of	DNA,infected
R3482	T4423	T4424	arg1Of	extracted,from
R3483	T4425	T4424	arg2Of	PBMC,from
R3484	T4428	T4427	arg2Of	infected,were
R3485	T4428	T4429	arg1Of	infected,with
R3486	T4432	T4429	arg2Of	isolate,with
R3487	T4432	T4430	arg1Of	isolate,a
R3488	T4432	T4431	arg1Of	isolate,primary
R3489	T4432	T4433	arg1Of	isolate,of
R3490	T4434	T4433	arg2Of	HIV-198IN22,of
R3491	T4435	T4436	arg1Of	HIV-1Lai/Bal-Env,and
R3492	T4437	T4436	arg2Of	HIV-198IN22,and
R3493	T4439	T4435	arg1Of	viruses,HIV-1Lai/Bal-Env
R3494	T4439	T4437	arg1Of	viruses,HIV-198IN22
R3495	T4439	T4438	arg1Of	viruses,mutant
R3496	T4439	T4440	arg1Of	viruses,were
R3497	T4439	T4441	arg2Of	viruses,constructed
R3498	T4441	T4440	arg2Of	constructed,were
R3499	T4441	T4442	arg1Of	constructed,by
R3500	T4443	T4442	arg2Of	introducing,by
R3501	T4445	T4443	arg2Of	mutations,introducing
R3502	T4445	T4444	arg1Of	mutations,point
R3503	T4445	T4446	arg1Of	mutations,using
R3504	T4450	T4446	arg2Of	methods,using
R3505	T4450	T4447	arg1Of	methods,standard
R3506	T4450	T4448	arg1Of	methods,PCR-based
R3507	T4450	T4449	arg1Of	methods,mutagenesis
R3645	T4671	T4670	arg1Of	transfection,siRNA
R3646	T4671	T4672	arg1Of	transfection,of
R3647	T4673	T4672	arg2Of	MDM,of
R3648	T4675	T4674	arg1Of	siRNA,An
R3649	T4675	T4676	arg1Of	siRNA,was
R3650	T4675	T4677	arg2Of	siRNA,constructed
R3651	T4677	T4676	arg2Of	constructed,was
R3652	T4677	T4678	arg1Of	constructed,(
R3653	T4677	T4684	modOf	constructed,to
R3654	T4677	T4693	arg1Of	constructed,:
R3655	T4680	T4678	arg2Of	Inc.,(
R3656	T4680	T4679	arg1Of	Inc.,Ambion
R3657	T4680	T4681	arg1Of	Inc.,","
R3658	T4682	T4681	arg2Of	www.ambion.com,","
R3659	T4683	T4678	arg3Of	),(
R3660	T4685	T4684	arg1Of	target,to
R3661	T4687	T4685	arg2Of	sequence,target
R3662	T4687	T4686	arg1Of	sequence,a
R3663	T4687	T4688	arg1Of	sequence,unique
R3664	T4688	T4689	arg1Of	unique,to
R3665	T4692	T4689	arg2Of	transcript,to
R3666	T4692	T4690	arg1Of	transcript,the
R3667	T4692	T4691	arg1Of	transcript,NFAT5
R3668	T4694	T4695	arg1Of	5′-CAACATGCCTGGAATTCAA-3′,(
R3669	T4694	T4701	arg1Of	5′-CAACATGCCTGGAATTCAA-3′,[
R3670	T4699	T4695	arg2Of	353,(
R3671	T4699	T4696	arg1Of	353,nt
R3672	T4699	T4697	arg1Of	353,335
R3673	T4699	T4698	arg1Of	353,to
R3674	T4700	T4695	arg3Of	),(
R3675	T4702	T4701	arg2Of	31,[
R3676	T4703	T4701	arg3Of	],[
R3677	T4705	T4704	arg2Of	described,As
R3678	T4705	T4706	arg1Of	described,","
R3679	T4708	T4706	arg2Of	control,","
R3680	T4708	T4707	arg1Of	control,a
R3681	T4708	T4709	arg1Of	control,for
R3682	T4712	T4709	arg2Of	effects,for
R3683	T4712	T4710	arg1Of	effects,non-specific
R3684	T4712	T4711	arg1Of	effects,siRNA
R3685	T4714	T4715	arg1Of	we,used
R3686	T4715	T4704	arg1Of	used,As
R3687	T4715	T4713	arg1Of	used,","
R3688	T4717	T4715	arg2Of	siRNA,used
R3689	T4717	T4716	arg1Of	siRNA,an
R3690	T4717	T4718	arg1Of	siRNA,targeting
R3691	T4722	T4718	arg2Of	protein,targeting
R3692	T4722	T4719	arg1Of	protein,the
R3693	T4722	T4720	arg1Of	protein,green
R3694	T4722	T4721	arg1Of	protein,fluorescent
R3695	T4722	T4723	arg1Of	protein,(
R3696	T4722	T4726	arg1Of	protein,","
R3697	T4724	T4723	arg2Of	GFP,(
R3698	T4725	T4723	arg3Of	),(
R3699	T4728	T4726	arg2Of	GGCTACGTCCAGGAGCGCACC-3′,","
R3700	T4728	T4727	arg1Of	GGCTACGTCCAGGAGCGCACC-3′,5′-
R3701	T4729	T4730	arg1Of	MDM,were
R3702	T4729	T4731	arg2Of	MDM,transfected
R3703	T4731	T4730	arg2Of	transfected,were
R3704	T4731	T4732	arg1Of	transfected,in
R3705	T4731	T4760	arg1Of	transfected,","
R3706	T4731	T4761	arg1Of	transfected,in
R3707	T4734	T4732	arg2Of	plates,in
R3708	T4734	T4733	arg1Of	plates,6-well
R3709	T4734	T4735	arg1Of	plates,using
R3710	T4734	T4753	arg1Of	plates,","
R3711	T4734	T4754	arg2Of	plates,prepared
R3712	T4737	T4735	arg2Of	µM,using
R3713	T4737	T4736	arg1Of	µM,1
R3714	T4737	T4738	arg1Of	µM,of
R3715	T4737	T4742	arg1Of	µM,in
R3716	T4741	T4738	arg2Of	siRNA,of
R3717	T4741	T4739	arg1Of	siRNA,the
R3718	T4741	T4740	arg2Of	siRNA,indicated
R3719	T4746	T4742	arg2Of	reagent,in
R3720	T4746	T4743	arg1Of	reagent,siPORT
R3721	T4746	T4744	arg1Of	reagent,NeoFX
R3722	T4746	T4745	arg1Of	reagent,transfection
R3723	T4746	T4747	arg1Of	reagent,(
R3724	T4749	T4747	arg2Of	Inc.,(
R3725	T4749	T4748	arg1Of	Inc.,Ambion
R3726	T4749	T4750	arg1Of	Inc.,","
R3727	T4751	T4750	arg2Of	www.ambion.com,","
R3728	T4752	T4747	arg3Of	),(
R3729	T4754	T4755	arg1Of	prepared,as
R3730	T4756	T4755	arg2Of	recommended,as
R3731	T4759	T4756	arg1Of	manufacturer,recommended
R3732	T4759	T4757	arg2Of	manufacturer,by
R3733	T4759	T4758	arg1Of	manufacturer,the
R3734	T4764	T4761	arg2Of	volume,in
R3735	T4764	T4762	arg1Of	volume,a
R3736	T4764	T4763	arg1Of	volume,final
R3737	T4764	T4765	arg1Of	volume,of
R3738	T4764	T4768	arg1Of	volume,in
R3739	T4767	T4765	arg2Of	µl,of
R3740	T4767	T4766	arg1Of	µl,750
R3741	T4770	T4769	arg1Of	medium,Macrophage-SFM
R3742	T4770	T4771	arg1Of	medium,plus
R3743	T4771	T4768	arg2Of	plus,in
R3744	T4772	T4773	arg1Of	5,%
R3745	T4777	T4771	arg2Of	serum,plus
R3746	T4777	T4772	arg1Of	serum,5
R3747	T4777	T4774	arg1Of	serum,heat-inactivated
R3748	T4777	T4775	arg1Of	serum,human
R3749	T4777	T4776	arg1Of	serum,AB
R3750	T4779	T4778	arg1Of	cultures,The
R3751	T4779	T4780	arg1Of	cultures,were
R3752	T4779	T4781	arg2Of	cultures,left
R3753	T4781	T4780	arg2Of	left,were
R3754	T4781	T4782	arg1Of	left,at
R3755	T4781	T4784	arg1Of	left,overnight
R3756	T4783	T4782	arg2Of	37°C,at
R3757	T4786	T4781	arg3Of	which,left
R3758	T4786	T4785	arg2Of	which,after
R3759	T4787	T4788	arg1Of	cells,were
R3760	T4787	T4789	arg2Of	cells,washed
R3761	T4787	T4791	arg2Of	cells,incubated
R3762	T4789	T4790	arg1Of	washed,and
R3763	T4790	T4785	arg1Of	and,after
R3764	T4790	T4788	arg2Of	and,were
R3765	T4790	T4792	arg1Of	and,in
R3766	T4791	T4790	arg2Of	incubated,and
R3767	T4794	T4792	arg2Of	medium,in
R3768	T4794	T4793	arg1Of	medium,fresh
R3769	T4795	T4796	arg1Of	MDM,were
R3770	T4795	T4797	arg2Of	MDM,transfected
R3771	T4797	T4796	arg2Of	transfected,were
R3772	T4797	T4799	arg1Of	transfected,times
R3773	T4797	T4800	arg1Of	transfected,for
R3774	T4797	T4803	arg1Of	transfected,down
R3775	T4797	T4807	arg1Of	transfected,before
R3776	T4799	T4798	arg1Of	times,two
R3777	T4802	T4800	arg2Of	knock,for
R3778	T4802	T4801	arg1Of	knock,efficient
R3779	T4803	T4804	arg1Of	down,of
R3780	T4806	T4804	arg2Of	expression,of
R3781	T4806	T4805	arg1Of	expression,NFAT5
R3782	T4809	T4808	arg1Of	experiments,infection
R3783	T4809	T4810	arg1Of	experiments,were
R3784	T4809	T4811	arg2Of	experiments,performed
R3785	T4811	T4807	arg2Of	performed,before
R3786	T4811	T4810	arg2Of	performed,were
R3787	T4811	T4812	arg1Of	performed,[
R3788	T4813	T4812	arg2Of	31,[
R3789	T4814	T4812	arg3Of	],[
R3983	T5111	T5109	arg1Of	cells,Stable
R3984	T5111	T5110	arg1Of	cells,THP-1
R3985	T5111	T5112	arg1Of	cells,expressing
R3986	T5113	T5112	arg2Of	shRNA,expressing
R3987	T5117	T5114	arg1Of	pLKO.1,The
R3988	T5117	T5115	arg1Of	pLKO.1,lentiviral
R3989	T5117	T5116	arg1Of	pLKO.1,plasmid
R3990	T5117	T5118	arg1Of	pLKO.1,expressing
R3991	T5117	T5123	arg1Of	pLKO.1,was
R3992	T5117	T5124	arg2Of	pLKO.1,purchased
R3993	T5117	T5132	arg1Of	pLKO.1,was
R3995	T5117	T5183	arg1Of	pLKO.1,were
R3996	T5117	T5184	arg2Of	pLKO.1,cloned
R3997	T5119	T5118	arg2Of	shRNA,expressing
R3998	T5119	T5120	arg1Of	shRNA,targeting
R3999	T5122	T5120	arg2Of	MyD88,targeting
R4000	T5122	T5121	arg1Of	MyD88,human
R4001	T5124	T5123	arg2Of	purchased,was
R4002	T5124	T5125	arg1Of	purchased,from
R4003	T5124	T5131	arg1Of	purchased,and
R4004	T5127	T5125	arg2Of	Biosystems,from
R4005	T5127	T5126	arg1Of	Biosystems,Open
R4006	T5127	T5128	arg1Of	Biosystems,(
R4007	T5129	T5128	arg2Of	www.openbiosystems.com,(
R4008	T5130	T5128	arg3Of	),(
R4009	T5133	T5132	arg2Of	validated,was
R4010	T5133	T5134	arg1Of	validated,in
R4011	T5133	T5182	arg1Of	validated,and
R4012	T5137	T5134	arg2Of	shRNA,in
R4013	T5137	T5135	arg1Of	shRNA,our
R4014	T5137	T5136	arg1Of	shRNA,laboratory.
R4015	T5137	T5138	arg1Of	shRNA,targeting
R4016	T5144	T5139	arg1Of	5′-CCGGAGCAGCTGTCCAGGTTTCGTCTCATAAAACCTGGACAGCTGCTCCTTTTTG-3′,human
R4017	T5144	T5140	arg1Of	5′-CCGGAGCAGCTGTCCAGGTTTCGTCTCATAAAACCTGGACAGCTGCTCCTTTTTG-3′,IRAK1
R4018	T5144	T5141	arg1Of	5′-CCGGAGCAGCTGTCCAGGTTTCGTCTCATAAAACCTGGACAGCTGCTCCTTTTTG-3′,(
R4019	T5144	T5142	arg1Of	5′-CCGGAGCAGCTGTCCAGGTTTCGTCTCATAAAACCTGGACAGCTGCTCCTTTTTG-3′,forward
R4020	T5144	T5143	arg1Of	5′-CCGGAGCAGCTGTCCAGGTTTCGTCTCATAAAACCTGGACAGCTGCTCCTTTTTG-3′,primer
R4021	T5144	T5145	arg1Of	5′-CCGGAGCAGCTGTCCAGGTTTCGTCTCATAAAACCTGGACAGCTGCTCCTTTTTG-3′,","
R4022	T5145	T5158	arg1Of	",",and
R4023	T5149	T5145	arg2Of	mRNA,","
R4024	T5149	T5146	arg1Of	mRNA,reverse
R4025	T5149	T5147	arg1Of	mRNA,primer
R4026	T5149	T5148	arg1Of	mRNA,5′-AATTCAAAAAGGAGCAGCTGTCCAGGTTTTATGAGACGAAACCTGGACAGCTGCT-3′
R4027	T5149	T5150	arg1Of	mRNA,(
R4028	T5154	T5151	arg1Of	sequence,IRAK1
R4029	T5154	T5152	arg1Of	sequence,mRNA
R4030	T5154	T5153	arg1Of	sequence,target
R4031	T5154	T5155	arg1Of	sequence,is
R4032	T5154	T5156	arg2Of	sequence,underlined
R4033	T5156	T5150	arg2Of	underlined,(
R4034	T5156	T5155	arg2Of	underlined,is
R4035	T5157	T5150	arg3Of	),(
R4036	T5158	T5138	arg2Of	and,targeting
R4037	T5158	T5177	modOf	and,were
R4038	T5164	T5158	arg2Of	5′-CCGGAGAAACCTGTTGTGATTCGTCTCATAAATCACAACAGGTTTCTCCTTTTTG-3′,and
R4039	T5164	T5159	arg1Of	5′-CCGGAGAAACCTGTTGTGATTCGTCTCATAAATCACAACAGGTTTCTCCTTTTTG-3′,human
R4040	T5164	T5160	arg1Of	5′-CCGGAGAAACCTGTTGTGATTCGTCTCATAAATCACAACAGGTTTCTCCTTTTTG-3′,TRAF6
R4041	T5164	T5161	arg1Of	5′-CCGGAGAAACCTGTTGTGATTCGTCTCATAAATCACAACAGGTTTCTCCTTTTTG-3′,(
R4042	T5164	T5162	arg1Of	5′-CCGGAGAAACCTGTTGTGATTCGTCTCATAAATCACAACAGGTTTCTCCTTTTTG-3′,forward
R4043	T5164	T5163	arg1Of	5′-CCGGAGAAACCTGTTGTGATTCGTCTCATAAATCACAACAGGTTTCTCCTTTTTG-3′,primer
R4044	T5168	T5166	arg1Of	5′-AATTCAAAAAGGAGAAACCTGTTGTGATTTATGAGACGAATCACAACAGGTTTCT-3′,reverse
R4045	T5168	T5167	arg1Of	5′-AATTCAAAAAGGAGAAACCTGTTGTGATTTATGAGACGAATCACAACAGGTTTCT-3′,primer
R4046	T5168	T5169	arg1Of	5′-AATTCAAAAAGGAGAAACCTGTTGTGATTTATGAGACGAATCACAACAGGTTTCT-3′,(
R4047	T5168	T5177	arg1Of	5′-AATTCAAAAAGGAGAAACCTGTTGTGATTTATGAGACGAATCACAACAGGTTTCT-3′,were
R4048	T5168	T5178	arg2Of	5′-AATTCAAAAAGGAGAAACCTGTTGTGATTTATGAGACGAATCACAACAGGTTTCT-3′,designed
R4049	T5173	T5170	arg1Of	sequence,TRAF6
R4050	T5173	T5171	arg1Of	sequence,mRNA
R4051	T5173	T5172	arg1Of	sequence,target
R4052	T5173	T5174	arg1Of	sequence,is
R4053	T5173	T5175	arg2Of	sequence,underlined
R4054	T5175	T5169	arg2Of	underlined,(
R4055	T5175	T5174	arg2Of	underlined,is
R4056	T5176	T5169	arg3Of	),(
R4057	T5178	T5165	arg1Of	designed,","
R4058	T5178	T5177	arg2Of	designed,were
R4059	T5178	T5179	arg1Of	designed,in
R4060	T5181	T5179	arg2Of	laboratory,in
R4061	T5181	T5180	arg1Of	laboratory,our
R4062	T5182	T5131	arg2Of	and,and
R4063	T5184	T5182	arg2Of	cloned,and
R4064	T5184	T5183	arg2Of	cloned,were
R4065	T5184	T5185	arg1Of	cloned,into
R4066	T5188	T5185	arg2Of	plasmid,into
R4067	T5188	T5186	arg1Of	plasmid,the
R4068	T5188	T5187	arg1Of	plasmid,pLKO.1
R4069	T5189	T5190	arg1Of	Lentiviruses,encoding
R4070	T5189	T5193	arg1Of	Lentiviruses,were
R4071	T5189	T5194	arg2Of	Lentiviruses,generated
R4072	T5192	T5190	arg2Of	sequences,encoding
R4073	T5192	T5191	arg1Of	sequences,shRNA
R4074	T5194	T5193	arg2Of	generated,were
R4075	T5194	T5195	arg1Of	generated,by
R4076	T5196	T5195	arg2Of	transfecting,by
R4077	T5196	T5202	arg1Of	transfecting,with
R4078	T5201	T5196	arg2Of	HEK-293T,transfecting
R4079	T5201	T5197	arg1Of	HEK-293T,the
R4080	T5201	T5198	arg1Of	HEK-293T,packaging
R4081	T5201	T5199	arg1Of	HEK-293T,cell
R4082	T5201	T5200	arg1Of	HEK-293T,line
R4083	T5206	T5202	arg2Of	plasmids,with
R4084	T5206	T5203	arg1Of	plasmids,the
R4085	T5206	T5204	arg1Of	plasmids,shRNA-encoding
R4086	T5206	T5205	arg1Of	plasmids,pLKO.1
R4087	T5206	T5207	arg1Of	plasmids,in
R4088	T5208	T5207	arg2Of	combination,in
R4089	T5208	T5209	arg1Of	combination,with
R4090	T5213	T5210	arg1Of	psPAX2,the
R4091	T5213	T5211	arg1Of	psPAX2,packaging
R4092	T5213	T5212	arg1Of	psPAX2,plasmid
R4093	T5213	T5214	arg1Of	psPAX2,and
R4094	T5214	T5209	arg2Of	and,with
R4095	T5218	T5214	arg2Of	pMD2.G,and
R4096	T5218	T5215	arg1Of	pMD2.G,the
R4097	T5218	T5216	arg1Of	pMD2.G,envelope
R4098	T5218	T5217	arg1Of	pMD2.G,plasmid
R4099	T5218	T5219	arg1Of	pMD2.G,using
R4100	T5222	T5219	arg2Of	reagent,using
R4101	T5222	T5220	arg1Of	reagent,Effectene
R4102	T5222	T5221	arg1Of	reagent,transfection
R4103	T5222	T5223	arg1Of	reagent,(
R4104	T5224	T5223	arg2Of	Qiagen,(
R4105	T5224	T5225	arg1Of	Qiagen,","
R4106	T5226	T5225	arg2Of	www.qiagen.com,","
R4107	T5227	T5223	arg3Of	),(
R4108	T5228	T5229	arg1Of	Supernatants,were
R4109	T5228	T5230	arg2Of	Supernatants,collected
R4110	T5228	T5235	arg2Of	Supernatants,clarified
R4111	T5230	T5229	arg2Of	collected,were
R4112	T5230	T5232	arg1Of	collected,hours
R4113	T5230	T5233	arg1Of	collected,post-transfection
R4114	T5230	T5234	arg1Of	collected,","
R4115	T5230	T5235	modOf	collected,clarified
R4116	T5230	T5239	arg1Of	collected,and
R4117	T5232	T5231	arg1Of	hours,48
R4118	T5237	T5235	arg1Of	centrifugation,clarified
R4119	T5237	T5236	arg2Of	centrifugation,by
R4120	T5239	T5238	arg1Of	and,","
R4121	T5240	T5241	arg1Of	stored,at
R4122	T5242	T5240	arg2Of	−80°C.,stored
R4123	T5242	T5245	modOf	−80°C.,were
R4124	T5244	T5243	arg1Of	cells,THP-1
R4125	T5244	T5245	arg1Of	cells,were
R4126	T5244	T5246	arg2Of	cells,transduced
R4127	T5244	T5252	arg1Of	cells,culturing
R4128	T5246	T5245	arg2Of	transduced,were
R4129	T5246	T5247	arg1Of	transduced,with
R4130	T5246	T5251	arg1Of	transduced,by
R4131	T5250	T5247	arg2Of	particles,with
R4132	T5250	T5248	arg1Of	particles,the
R4133	T5250	T5249	arg1Of	particles,lentiviral
R4134	T5252	T5251	arg2Of	culturing,by
R4135	T5252	T5255	arg1Of	culturing,with
R4136	T5252	T5261	arg1Of	culturing,in
R4137	T5254	T5252	arg2Of	cells,culturing
R4138	T5254	T5253	arg1Of	cells,the
R4139	T5256	T5255	arg2Of	supernatants,with
R4140	T5256	T5257	arg1Of	supernatants,from
R4141	T5260	T5257	arg2Of	cells,from
R4142	T5260	T5258	arg1Of	cells,the
R4143	T5260	T5259	arg1Of	cells,virus-producing
R4144	T5261	T5262	arg1Of	in,the
R4145	T5261	T5264	arg1Of	in,of
R4146	T5263	T5261	arg2Of	presence,in
R4147	T5267	T5265	arg1Of	polybrene,8
R4148	T5267	T5266	arg1Of	polybrene,µg/ml
R4149	T5267	T5268	arg1Of	polybrene,(
R4150	T5267	T5273	arg1Of	polybrene,and
R4151	T5269	T5268	arg2Of	Millipore,(
R4152	T5269	T5270	arg1Of	Millipore,","
R4153	T5271	T5270	arg2Of	www.millipore.com,","
R4154	T5272	T5268	arg3Of	),(
R4155	T5273	T5242	arg1Of	and,−80°C.
R4156	T5273	T5278	arg1Of	and,at
R4157	T5274	T5273	arg2Of	spinoculation,and
R4158	T5274	T5275	arg1Of	spinoculation,for
R4159	T5277	T5275	arg2Of	hours,for
R4160	T5277	T5276	arg1Of	hours,two
R4161	T5278	T5239	arg2Of	at,and
R4162	T5280	T5278	arg2Of	RPM,at
R4163	T5280	T5279	arg1Of	RPM,2000
R4164	T5282	T5281	arg1Of	transduced,Successfully
R4165	T5283	T5282	arg1Of	cells,transduced
R4166	T5283	T5284	arg1Of	cells,were
R4167	T5283	T5285	arg2Of	cells,selected
R4168	T5283	T5287	arg2Of	cells,expanded
R4169	T5285	T5286	arg1Of	selected,and
R4170	T5286	T5284	arg2Of	and,were
R4171	T5287	T5286	arg2Of	expanded,and
R4172	T5289	T5285	arg1Of	treatment,selected
R4173	T5289	T5287	arg1Of	treatment,expanded
R4174	T5289	T5288	arg2Of	treatment,by
R4175	T5289	T5290	arg1Of	treatment,with
R4176	T5291	T5292	arg1Of	0.8,µg/ml
R4177	T5293	T5290	arg2Of	puromycin,with
R4178	T5293	T5291	arg1Of	puromycin,0.8
R4410	T5611	T5610	arg1Of	culture,MTb
R4411	T5616	T5612	arg1Of	CDC1551,The
R4412	T5616	T5613	arg1Of	CDC1551,MTb
R4413	T5616	T5614	arg1Of	CDC1551,clinical
R4414	T5616	T5615	arg1Of	CDC1551,strain
R4415	T5616	T5617	arg1Of	CDC1551,was
R4416	T5616	T5618	arg2Of	CDC1551,prepared
R4417	T5618	T5617	arg2Of	prepared,was
R4418	T5618	T5619	arg1Of	prepared,by
R4419	T5620	T5619	arg2Of	adding,by
R4420	T5622	T5620	arg2Of	µl,adding
R4421	T5622	T5621	arg1Of	µl,100
R4422	T5622	T5623	arg1Of	µl,of
R4423	T5622	T5627	arg1Of	µl,into
R4424	T5622	T5640	arg2Of	µl,supplemented
R4425	T5626	T5623	arg2Of	stock,of
R4426	T5626	T5624	arg2Of	stock,frozen
R4427	T5626	T5625	arg1Of	stock,bacteria
R4428	T5629	T5627	arg2Of	ml,into
R4429	T5629	T5628	arg1Of	ml,10
R4430	T5629	T5630	arg1Of	ml,of
R4431	T5633	T5630	arg2Of	medium,of
R4432	T5633	T5631	arg1Of	medium,Middlebrook
R4433	T5633	T5632	arg1Of	medium,7H9
R4434	T5633	T5634	arg1Of	medium,(
R4435	T5636	T5634	arg2Of	BD,(
R4436	T5636	T5635	arg1Of	BD,Difco
R4437	T5636	T5637	arg1Of	BD,","
R4438	T5638	T5637	arg2Of	www.bd.com,","
R4439	T5639	T5634	arg3Of	),(
R4440	T5640	T5641	arg1Of	supplemented,with
R4441	T5644	T5642	arg1Of	complex,albumin
R4442	T5644	T5643	arg1Of	complex,dextrose
R4443	T5644	T5645	arg1Of	complex,(
R4444	T5644	T5648	arg1Of	complex,and
R4445	T5646	T5645	arg2Of	ADC,(
R4446	T5647	T5645	arg3Of	),(
R4447	T5648	T5641	arg2Of	and,with
R4448	T5650	T5649	arg1Of	%,0.05
R4449	T5652	T5648	arg2Of	80,and
R4450	T5652	T5650	arg1Of	80,%
R4451	T5652	T5651	arg1Of	80,Tween
R4452	T5652	T5653	arg1Of	80,(
R4453	T5654	T5653	arg2Of	Sigma-Aldrich,(
R4454	T5654	T5655	arg1Of	Sigma-Aldrich,","
R4455	T5656	T5655	arg2Of	www.sigmaaldrich.com,","
R4456	T5657	T5653	arg3Of	),(
R4457	T5659	T5658	arg1Of	cultures,The
R4458	T5659	T5660	arg1Of	cultures,were
R4459	T5659	T5661	arg2Of	cultures,grown
R4460	T5659	T5670	arg1Of	cultures,ensure
R4461	T5661	T5660	arg2Of	grown,were
R4462	T5661	T5662	arg1Of	grown,to
R4463	T5661	T5667	arg1Of	grown,at
R4464	T5661	T5669	modOf	grown,to
R4465	T5664	T5662	arg2Of	OD650,to
R4466	T5664	T5663	arg1Of	OD650,an
R4467	T5664	T5665	arg1Of	OD650,of
R4468	T5666	T5665	arg2Of	0.4,of
R4469	T5668	T5667	arg2Of	37°C,at
R4470	T5670	T5669	arg1Of	ensure,to
R4471	T5672	T5673	arg1Of	they,were
R4472	T5672	T5674	arg1Of	they,in
R4473	T5673	T5670	arg2Of	were,ensure
R4474	T5673	T5671	arg1Of	were,that
R4475	T5674	T5673	arg2Of	in,were
R4476	T5678	T5674	arg2Of	phase,in
R4477	T5678	T5675	arg1Of	phase,the
R4478	T5678	T5676	arg1Of	phase,logarithmic
R4479	T5678	T5677	arg1Of	phase,growth
R4480	T5679	T5680	arg1Of	Bacteria,were
R4481	T5679	T5682	arg2Of	Bacteria,plated
R4482	T5679	T5684	arg2Of	Bacteria,washed
R4483	T5682	T5683	arg1Of	plated,","
R4484	T5683	T5680	arg2Of	",",were
R4485	T5683	T5681	arg1Of	",",then
R4486	T5684	T5683	arg2Of	washed,","
R4487	T5684	T5685	arg1Of	washed,with
R4488	T5686	T5685	arg2Of	PBS,with
R4489	T5686	T5688	arg2Of	PBS,resuspended
R4490	T5686	T5693	arg2Of	PBS,passed
R4491	T5688	T5689	arg1Of	resuspended,in
R4492	T5688	T5692	arg1Of	resuspended,and
R4493	T5690	T5689	arg2Of	PBS,in
R4494	T5692	T5687	arg1Of	and,","
R4495	T5692	T5691	arg1Of	and,","
R4496	T5693	T5692	arg2Of	passed,and
R4497	T5693	T5694	arg1Of	passed,through
R4498	T5698	T5694	arg2Of	filter,through
R4499	T5698	T5695	arg1Of	filter,a
R4500	T5698	T5696	arg1Of	filter,5
R4501	T5698	T5697	arg1Of	filter,µm
R4502	T5698	T5699	modOf	filter,to
R4503	T5698	T5700	arg1Of	filter,ensure
R4504	T5700	T5699	arg1Of	ensure,to
R4505	T5703	T5702	arg1Of	bacteria,the
R4506	T5703	T5704	arg1Of	bacteria,were
R4507	T5703	T5705	arg1Of	bacteria,in
R4508	T5704	T5700	arg2Of	were,ensure
R4509	T5704	T5701	arg1Of	were,that
R4510	T5705	T5704	arg2Of	in,were
R4511	T5709	T5705	arg2Of	suspension,in
R4512	T5709	T5706	arg1Of	suspension,a
R4513	T5709	T5707	arg1Of	suspension,single
R4514	T5709	T5708	arg1Of	suspension,cell
R4515	T5712	T5710	arg1Of	numbers,Bacterial
R4516	T5712	T5711	arg1Of	numbers,cell
R4517	T5712	T5713	arg1Of	numbers,were
R4518	T5712	T5714	arg2Of	numbers,determined
R4519	T5714	T5713	arg2Of	determined,were
R4520	T5716	T5714	arg1Of	measurement,determined
R4521	T5716	T5715	arg2Of	measurement,by
R4522	T5716	T5717	arg1Of	measurement,of
R4523	T5718	T5717	arg2Of	OD650,of
R4524	T5718	T5719	arg1Of	OD650,before
R4525	T5721	T5719	arg2Of	dilution,before
R4526	T5721	T5720	arg1Of	dilution,further
R4527	T5721	T5722	arg1Of	dilution,with
R4528	T5725	T5722	arg2Of	medium,with
R4529	T5725	T5723	arg1Of	medium,RPMI
R4530	T5725	T5724	arg1Of	medium,1640
R4531	T5725	T5726	arg1Of	medium,for
R4532	T5725	T5730	arg1Of	medium,at
R4533	T5729	T5726	arg2Of	studies,for
R4534	T5729	T5727	arg1Of	studies,cell
R4535	T5729	T5728	arg1Of	studies,infection
R4536	T5733	T5731	arg1Of	bacilli,10∶1
R4537	T5733	T5732	arg1Of	bacilli,PBMC∶
R4538	T5733	T5734	arg1Of	bacilli,or
R4539	T5734	T5730	arg2Of	or,at
R4540	T5737	T5735	arg1Of	bacilli,1∶1
R4541	T5737	T5736	arg1Of	bacilli,MDM∶
R4542	T5737	T5738	arg1Of	bacilli,and
R4543	T5738	T5734	arg2Of	and,or
R4544	T5739	T5738	arg2Of	THP-1∶bacilli,and
R4545	T5743	T5742	arg2Of	CFU,(
R4546	T5744	T5742	arg3Of	),(
R4547	T5745	T5740	arg1Of	analysis,Colony-forming
R4548	T5745	T5741	arg1Of	analysis,unit
R4549	T5745	T5742	arg1Of	analysis,(
R4550	T5745	T5746	arg1Of	analysis,was
R4551	T5745	T5747	arg2Of	analysis,performed
R4552	T5747	T5746	arg2Of	performed,was
R4553	T5747	T5748	arg1Of	performed,and
R4554	T5747	T5749	arg1Of	performed,on
R4555	T5751	T5752	arg1Of	4,and
R4556	T5752	T5749	arg2Of	and,on
R4557	T5752	T5750	arg1Of	and,days
R4558	T5753	T5752	arg2Of	7,and
R4559	T5757	T5754	arg1Of	counts,the
R4560	T5757	T5755	arg1Of	counts,average
R4561	T5757	T5756	arg1Of	counts,CFU
R4562	T5757	T5758	arg1Of	counts,were
R4563	T5758	T5746	modOf	were,was
R4564	T5758	T5762	arg1Of	were,","
R4565	T5758	T5763	arg1Of	were,respectively
R4566	T5758	T5764	arg1Of	were,","
R4567	T5758	T5765	modOf	were,confirming
R4568	T5759	T5760	arg1Of	6×103,and
R4569	T5760	T5758	arg2Of	and,were
R4570	T5761	T5760	arg2Of	5×104,and
R4571	T5768	T5767	arg1Of	levels,mycobacteria
R4572	T5768	T5769	arg1Of	levels,increased
R4573	T5769	T5765	arg2Of	increased,confirming
R4574	T5769	T5766	arg1Of	increased,that
R4575	T5769	T5770	arg1Of	increased,over
R4576	T5772	T5770	arg2Of	course,over
R4577	T5772	T5771	arg1Of	course,the
R4578	T5772	T5773	arg1Of	course,of
R4579	T5774	T5773	arg2Of	infection,of
R4580	T5774	T5775	arg1Of	infection,of
R4581	T5777	T5775	arg2Of	MDM,of
R4582	T5777	T5776	arg1Of	MDM,primary
R4780	T6025	T6037	arg1Of	buffer,","
R4792	T6038	T6039	arg1Of	1,%
R4793	T6040	T6038	arg1Of	Triton,1
R4794	T6040	T6041	arg1Of	Triton,","
R4795	T6041	T6046	arg1Of	",",and
R4796	T6043	T6042	arg1Of	%,10
R4797	T6044	T6041	arg2Of	glycerol,","
R4798	T6044	T6043	arg1Of	glycerol,%
R4799	T6046	T6037	arg2Of	and,","
R4800	T6046	T6045	arg1Of	and,","
R4801	T6046	T6049	arg1Of	and,of
R4802	T6048	T6046	arg2Of	tablet,and
R4803	T6048	T6047	arg1Of	tablet,1
R4804	T6054	T6050	arg1Of	Cocktail,Complete
R4805	T6054	T6051	arg1Of	Cocktail,EDTA-free
R4806	T6054	T6052	arg1Of	Cocktail,Protease
R4807	T6054	T6053	arg1Of	Cocktail,Inhibitor
R4808	T6054	T6055	arg1Of	Cocktail,(
R4809	T6054	T6058	arg1Of	Cocktail,per
R4810	T6056	T6055	arg2Of	Roche,(
R4811	T6057	T6055	arg3Of	),(
R4812	T6060	T6058	arg2Of	ml,per
R4813	T6060	T6059	arg1Of	ml,25
R4814	T6062	T6049	arg2Of	buffer,of
R4815	T6062	T6054	arg1Of	buffer,Cocktail
R4816	T6062	T6061	arg1Of	buffer,of
R4817	T6063	T6064	arg1Of	Extracts,were
R4818	T6063	T6065	arg2Of	Extracts,boiled
R4819	T6065	T6064	arg2Of	boiled,were
R4820	T6065	T6066	arg1Of	boiled,for
R4821	T6065	T6079	arg1Of	boiled,and
R4822	T6068	T6066	arg2Of	min,for
R4823	T6068	T6067	arg1Of	min,5
R4824	T6068	T6069	arg1Of	min,in
R4825	T6068	T6074	arg1Of	min,with
R4826	T6073	T6069	arg2Of	buffer,in
R4827	T6073	T6070	arg1Of	buffer,1×
R4828	T6073	T6071	arg1Of	buffer,Laemmli
R4829	T6073	T6072	arg1Of	buffer,sample
R4830	T6075	T6076	arg1Of	5,%
R4831	T6078	T6074	arg2Of	2-mercaptoethanol,with
R4832	T6078	T6075	arg1Of	2-mercaptoethanol,5
R4833	T6078	T6077	arg1Of	2-mercaptoethanol,v/v
R4834	T6080	T6081	arg1Of	proteins,were
R4835	T6080	T6082	arg2Of	proteins,separated
R4836	T6082	T6079	arg2Of	separated,and
R4837	T6082	T6081	arg2Of	separated,were
R4838	T6084	T6082	arg1Of	SDS-PAGE,separated
R4839	T6084	T6083	arg2Of	SDS-PAGE,by
R4840	T6086	T6085	arg1Of	gel,The
R4841	T6086	T6087	arg1Of	gel,was
R4842	T6086	T6088	arg2Of	gel,transferred
R4843	T6088	T6087	arg2Of	transferred,was
R4844	T6088	T6089	arg1Of	transferred,to
R4845	T6094	T6089	arg2Of	Membrane,to
R4846	T6094	T6090	arg1Of	Membrane,a
R4847	T6094	T6091	arg1Of	Membrane,nitrocellulose
R4848	T6094	T6092	arg1Of	Membrane,Trans-Blot
R4849	T6094	T6093	arg1Of	Membrane,Transfer
R4850	T6094	T6095	arg1Of	Membrane,(
R4851	T6096	T6095	arg2Of	BioRad,(
R4852	T6097	T6095	arg3Of	),(
R4853	T6099	T6098	arg1Of	blot,The
R4854	T6099	T6100	arg1Of	blot,was
R4855	T6099	T6102	arg2Of	blot,blocked
R4856	T6102	T6100	arg2Of	blocked,was
R4857	T6102	T6101	arg1Of	blocked,then
R4858	T6102	T6103	arg1Of	blocked,for
R4859	T6102	T6106	arg1Of	blocked,at
R4860	T6102	T6108	arg1Of	blocked,in
R4861	T6105	T6103	arg2Of	h,for
R4862	T6105	T6104	arg1Of	h,1
R4863	T6107	T6106	arg2Of	37°C,at
R4864	T6110	T6108	arg2Of	solution,in
R4865	T6110	T6109	arg1Of	solution,a
R4866	T6110	T6111	arg1Of	solution,of
R4867	T6112	T6113	arg1Of	4,%
R4868	T6114	T6112	arg1Of	BSA,4
R4869	T6114	T6115	arg1Of	BSA,(
R4870	T6114	T6118	arg1Of	BSA,and
R4871	T6116	T6115	arg2Of	Sigma,(
R4872	T6117	T6115	arg3Of	),(
R4873	T6118	T6111	arg2Of	and,of
R4874	T6118	T6125	arg1Of	and,in
R4875	T6120	T6118	arg2Of	%,and
R4876	T6120	T6119	arg1Of	%,0.1
R4877	T6120	T6121	arg1Of	%,Tween-20
R4878	T6120	T6122	arg1Of	%,(
R4879	T6123	T6122	arg2Of	BioRad,(
R4880	T6124	T6122	arg3Of	),(
R4881	T6127	T6125	arg2Of	buffer,in
R4882	T6127	T6126	arg1Of	buffer,a
R4883	T6127	T6128	arg1Of	buffer,containing
R4884	T6131	T6129	arg1Of	Tris,50
R4885	T6131	T6130	arg1Of	Tris,mM
R4886	T6131	T6132	arg1Of	Tris,and
R4887	T6132	T6128	arg2Of	and,containing
R4888	T6132	T6136	arg1Of	and,at
R4889	T6133	T6134	arg1Of	150,mM
R4890	T6135	T6132	arg2Of	NaCl,and
R4891	T6135	T6133	arg1Of	NaCl,150
R4892	T6137	T6136	arg2Of	pH,at
R4893	T6137	T6138	arg1Of	pH,7.6
R4894	T6137	T6139	arg1Of	pH,(
R4895	T6140	T6139	arg2Of	BSA/TBST,(
R4896	T6141	T6139	arg3Of	),(
R4897	T6143	T6142	arg1Of	incubation,Primary
R4898	T6143	T6144	arg1Of	incubation,was
R4899	T6143	T6145	arg2Of	incubation,carried
R4900	T6145	T6144	arg2Of	carried,was
R4901	T6145	T6146	arg1Of	carried,out
R4902	T6145	T6147	arg1Of	carried,with
R4903	T6149	T6147	arg2Of	dilution,with
R4904	T6149	T6148	arg1Of	dilution,a1∶200
R4905	T6149	T6150	arg1Of	dilution,of
R4906	T6153	T6151	arg1Of	antibody,rabbit
R4907	T6153	T6152	arg1Of	antibody,anti-NFAT5
R4908	T6153	T6154	arg1Of	antibody,(
R4909	T6153	T6157	arg1Of	antibody,(
R4910	T6153	T6162	arg1Of	antibody,and
R4911	T6155	T6154	arg2Of	H-300,(
R4912	T6156	T6154	arg3Of	),(
R4913	T6160	T6157	arg2Of	Biotechnology,(
R4914	T6160	T6158	arg1Of	Biotechnology,Santa
R4915	T6160	T6159	arg1Of	Biotechnology,Cruz
R4916	T6161	T6157	arg3Of	),(
R4917	T6162	T6150	arg2Of	and,of
R4918	T6165	T6162	arg2Of	dilution,and
R4919	T6165	T6163	arg1Of	dilution,a
R4920	T6165	T6164	arg1Of	dilution,1∶500
R4921	T6165	T6166	arg1Of	dilution,of
R4922	T6165	T6177	arg1Of	dilution,in
R4923	T6169	T6166	arg2Of	antibody,of
R4924	T6169	T6167	arg1Of	antibody,goat
R4925	T6169	T6168	arg1Of	antibody,anti-Lamin-B1
R4926	T6169	T6170	arg1Of	antibody,(
R4927	T6171	T6170	arg2Of	sc-6217,(
R4928	T6171	T6172	arg1Of	sc-6217,;
R4929	T6175	T6172	arg2Of	Biotechnology,;
R4930	T6175	T6173	arg1Of	Biotechnology,Santa
R4931	T6175	T6174	arg1Of	Biotechnology,Cruz
R4932	T6176	T6170	arg3Of	),(
R4933	T6178	T6177	arg2Of	BSA/TBST,in
R4934	T6178	T6179	arg1Of	BSA/TBST,for
R4935	T6181	T6179	arg2Of	h,for
R4936	T6181	T6180	arg1Of	h,2
R4937	T6181	T6182	arg1Of	h,at
R4938	T6184	T6182	arg2Of	temperature,at
R4939	T6184	T6183	arg1Of	temperature,room
R4940	T6186	T6185	arg1Of	blot,The
R4941	T6186	T6187	arg1Of	blot,was
R4942	T6186	T6188	arg2Of	blot,washed
R4943	T6186	T6194	arg2Of	blot,incubated
R4944	T6188	T6191	arg1Of	washed,in
R4945	T6188	T6193	arg1Of	washed,and
R4946	T6190	T6188	arg3Of	min,washed
R4947	T6190	T6189	arg1Of	min,3×5
R4948	T6192	T6191	arg2Of	TBST,in
R4949	T6193	T6187	arg2Of	and,was
R4950	T6194	T6193	arg2Of	incubated,and
R4951	T6194	T6195	arg1Of	incubated,in
R4952	T6194	T6210	arg1Of	incubated,as
R4953	T6198	T6196	arg1Of	anti-goat-HRP,1∶6000
R4954	T6198	T6197	arg1Of	anti-goat-HRP,donkey
R4955	T6198	T6199	arg1Of	anti-goat-HRP,(
R4956	T6198	T6204	arg1Of	anti-goat-HRP,or
R4957	T6202	T6199	arg2Of	Biotechnology,(
R4958	T6202	T6200	arg1Of	Biotechnology,Santa
R4959	T6202	T6201	arg1Of	Biotechnology,Cruz
R4960	T6203	T6199	arg3Of	),(
R4961	T6204	T6195	arg2Of	or,in
R4962	T6206	T6204	arg2Of	anti-rabbit-HRP,or
R4963	T6206	T6205	arg1Of	anti-rabbit-HRP,goat
R4964	T6206	T6207	arg1Of	anti-rabbit-HRP,(
R4965	T6208	T6207	arg2Of	BioRad,(
R4966	T6209	T6207	arg3Of	),(
R4967	T6211	T6210	arg2Of	appropriate,as
R4968	T6211	T6212	arg1Of	appropriate,for
R4969	T6214	T6212	arg2Of	h,for
R4970	T6214	T6213	arg1Of	h,1
R4971	T6216	T6215	arg1Of	blot,The
R4972	T6216	T6217	arg1Of	blot,was
R4973	T6216	T6219	arg2Of	blot,washed
R4974	T6216	T6225	arg1Of	blot,developed
R4975	T6219	T6217	arg2Of	washed,was
R4976	T6219	T6218	arg1Of	washed,again
R4977	T6219	T6222	arg1Of	washed,in
R4978	T6219	T6224	arg1Of	washed,and
R4979	T6221	T6219	arg3Of	min,washed
R4980	T6221	T6220	arg1Of	min,3×5
R4981	T6223	T6222	arg2Of	TBST,in
R4982	T6225	T6224	arg2Of	developed,and
R4983	T6225	T6226	arg1Of	developed,with
R4984	T6228	T6226	arg2Of	West,with
R4985	T6228	T6227	arg1Of	West,SuperSignal
R4986	T6231	T6225	arg2Of	Reagent,developed
R4987	T6231	T6229	arg1Of	Reagent,Pico
R4988	T6231	T6230	arg1Of	Reagent,Chemiluminescent
R4989	T6231	T6232	arg1Of	Reagent,(
R4990	T6233	T6232	arg2Of	Pierce,(
R4991	T6234	T6232	arg3Of	),(
R5232	T6537	T6536	arg1Of	PCR,Quantitative
R5233	T6541	T6538	arg1Of	levels,The
R5234	T6541	T6539	arg1Of	levels,mRNA
R5235	T6541	T6540	arg1Of	levels,expression
R5236	T6541	T6542	arg1Of	levels,were
R5237	T6541	T6543	arg2Of	levels,determined
R5238	T6543	T6542	arg2Of	determined,were
R5239	T6543	T6544	arg1Of	determined,by
R5240	T6548	T6544	arg2Of	PCR,by
R5241	T6548	T6545	arg1Of	PCR,SYBR
R5242	T6548	T6546	arg1Of	PCR,Green-based
R5243	T6548	T6547	arg1Of	PCR,real-time
R5244	T6548	T6549	arg1Of	PCR,(
R5245	T6551	T6549	arg2Of	Biosystems,(
R5246	T6551	T6550	arg1Of	Biosystems,Applied
R5247	T6551	T6552	arg1Of	Biosystems,","
R5248	T6553	T6552	arg2Of	www.appliedbiosystems.com,","
R5249	T6554	T6549	arg3Of	),(
R5250	T6557	T6555	arg1Of	conditions,The
R5251	T6557	T6556	arg1Of	conditions,reaction
R5252	T6557	T6558	arg1Of	conditions,were
R5253	T6558	T6560	arg1Of	were,for
R5254	T6558	T6563	modOf	were,followed
R5255	T6559	T6558	arg2Of	95°C,were
R5256	T6562	T6560	arg2Of	min,for
R5257	T6562	T6561	arg1Of	min,10
R5258	T6566	T6563	arg1Of	cycles,followed
R5259	T6566	T6564	arg2Of	cycles,by
R5260	T6566	T6565	arg1Of	cycles,40
R5261	T6566	T6567	arg1Of	cycles,of
R5262	T6568	T6567	arg2Of	95°C,of
R5263	T6568	T6569	arg1Of	95°C,for
R5265	T6571	T6572	arg1Of	sec,and
R5266	T6572	T6569	arg2Of	and,for
R5267	T6572	T6570	arg1Of	and,15
R5268	T6573	T6572	arg2Of	60°C,and
R5286	T6592	T6590	arg2Of	values,as
R5287	T6592	T6591	arg1Of	values,relative
R5381	T6705	T6704	arg1Of	aid,the
R5382	T6705	T6706	arg1Of	aid,of
R5383	T6708	T6706	arg2Of	Excel,of
R5384	T6708	T6707	arg1Of	Excel,Microsoft
R5385	T6710	T6709	arg1Of	p≤0.05,software.
R5386	T6710	T6711	arg1Of	p≤0.05,was
R5387	T6710	T6712	arg2Of	p≤0.05,considered
R5388	T6710	T6713	arg1Of	p≤0.05,significant
R5389	T6712	T6696	modOf	considered,was
R5390	T6712	T6711	arg2Of	considered,was
R5391	T6713	T6712	arg3Of	significant,considered
R5432	T6796	T6797	arg1Of	MTb,increases
R5433	T6800	T6797	arg2Of	activity,increases
R5434	T6800	T6798	arg1Of	activity,HIV-1
R5435	T6800	T6799	arg1Of	activity,LTR
R5436	T6800	T6801	arg1Of	activity,of
R5437	T6804	T6805	arg1Of	B,","
R5438	T6805	T6808	arg1Of	",",and
R5439	T6806	T6805	arg2Of	C,","
R5440	T6808	T6801	arg2Of	and,of
R5441	T6808	T6802	arg1Of	and,HIV-1
R5442	T6808	T6803	arg1Of	and,subtypes
R5443	T6808	T6807	arg1Of	and,","
R5444	T6809	T6808	arg2Of	E,and
R5445	T6811	T6810	arg1Of	compare,To
R5446	T6814	T6811	arg2Of	impact,compare
R5447	T6814	T6812	arg1Of	impact,the
R5448	T6814	T6813	arg1Of	impact,functional
R5449	T6814	T6815	arg1Of	impact,of
R5450	T6814	T6818	arg1Of	impact,on
R5451	T6817	T6815	arg2Of	stimulation,of
R5452	T6817	T6816	arg1Of	stimulation,MTb
R5453	T6822	T6818	arg2Of	activity,on
R5454	T6822	T6819	arg1Of	activity,subtype-specific
R5455	T6822	T6820	arg1Of	activity,HIV-1
R5456	T6822	T6821	arg1Of	activity,LTR
R5457	T6824	T6811	arg1Of	we,compare
R5458	T6824	T6826	arg1Of	we,constructed
R5459	T6826	T6810	modOf	constructed,To
R5460	T6826	T6823	arg1Of	constructed,","
R5461	T6826	T6825	arg1Of	constructed,first
R5462	T6828	T6826	arg2Of	plasmids,constructed
R5463	T6828	T6827	arg1Of	plasmids,reporter
R5464	T6828	T6829	arg1Of	plasmids,containing
R5465	T6832	T6833	arg1Of	B,","
R5466	T6833	T6836	arg1Of	",",and
R5467	T6834	T6833	arg2Of	C,","
R5468	T6836	T6835	arg1Of	and,","
R5469	T6837	T6836	arg2Of	E,and
R5470	T6838	T6829	arg2Of	LTRs,containing
R5471	T6838	T6830	arg1Of	LTRs,viral
R5472	T6838	T6831	arg1Of	LTRs,subtype
R5473	T6838	T6832	arg1Of	LTRs,B
R5474	T6838	T6834	arg1Of	LTRs,C
R5475	T6838	T6837	arg1Of	LTRs,E
R5476	T6838	T6839	arg1Of	LTRs,(
R5477	T6838	T6852	arg2Of	LTRs,linked
R5478	T6840	T6839	arg2Of	−208,(
R5479	T6840	T6841	arg1Of	−208,to
R5480	T6840	T6845	arg1Of	−208,relative
R5481	T6843	T6842	arg1Of	64,+
R5482	T6844	T6841	arg2Of	nt,to
R5483	T6844	T6843	arg1Of	nt,64
R5484	T6845	T6846	arg1Of	relative,to
R5485	T6850	T6846	arg2Of	site,to
R5486	T6850	T6847	arg1Of	site,the
R5487	T6850	T6848	arg1Of	site,transcription
R5488	T6850	T6849	arg1Of	site,start
R5489	T6851	T6839	arg3Of	),(
R5490	T6852	T6853	arg1Of	linked,to
R5491	T6858	T6853	arg2Of	gene,to
R5492	T6858	T6854	arg1Of	gene,the
R5493	T6858	T6855	arg1Of	gene,firefly
R5494	T6858	T6856	arg1Of	gene,luciferase
R5495	T6858	T6857	arg1Of	gene,reporter
R5496	T6860	T6859	arg2Of	transfection,After
R5497	T6860	T6861	arg1Of	transfection,of
R5498	T6866	T6861	arg2Of	line,of
R5499	T6866	T6862	arg1Of	line,the
R5500	T6866	T6863	arg1Of	line,monocytic
R5501	T6866	T6864	arg1Of	line,THP-1
R5502	T6866	T6865	arg1Of	line,cell
R5503	T6866	T6867	arg1Of	line,with
R5504	T6869	T6867	arg2Of	plasmids,with
R5505	T6869	T6868	arg1Of	plasmids,these
R5506	T6871	T6872	arg1Of	cells,were
R5507	T6871	T6873	arg2Of	cells,stimulated
R5508	T6873	T6859	arg1Of	stimulated,After
R5509	T6873	T6870	arg1Of	stimulated,","
R5510	T6873	T6872	arg2Of	stimulated,were
R5511	T6873	T6874	arg1Of	stimulated,with
R5512	T6879	T6874	arg2Of	lysate,with
R5513	T6879	T6875	arg1Of	lysate,an
R5514	T6879	T6876	arg1Of	lysate,irradiated
R5515	T6879	T6877	arg1Of	lysate,whole
R5516	T6879	T6878	arg1Of	lysate,cell
R5517	T6879	T6880	arg1Of	lysate,of
R5518	T6881	T6880	arg2Of	MTb,of
R5519	T6881	T6882	arg1Of	MTb,(
R5520	T6883	T6882	arg2Of	H37Rv,(
R5521	T6884	T6882	arg3Of	),(
R5522	T6885	T6886	arg1Of	We,note
R5523	T6889	T6888	arg1Of	lysate,MTb
R5524	T6889	T6890	arg1Of	lysate,induces
R5525	T6890	T6886	arg2Of	induces,note
R5526	T6890	T6887	arg1Of	induces,that
R5527	T6890	T6904	arg1Of	induces,(
R5528	T6892	T6890	arg2Of	responses,induces
R5529	T6892	T6891	arg1Of	responses,inflammatory
R5530	T6892	T6893	arg1Of	responses,in
R5531	T6894	T6893	arg2Of	monocytes,in
R5532	T6894	T6895	arg1Of	monocytes,that
R5533	T6894	T6896	arg1Of	monocytes,resemble
R5534	T6897	T6896	arg2Of	those,resemble
R5535	T6897	T6898	arg2Of	those,induced
R5536	T6898	T6899	arg1Of	induced,in
R5537	T6900	T6899	arg2Of	response,in
R5538	T6900	T6901	modOf	response,to
R5539	T6902	T6901	arg1Of	live,to
R5540	T6903	T6902	arg2Of	MTb,live
R5541	T6905	T6904	arg2Of	see,(
R5542	T6905	T6906	arg1Of	see,for
R5543	T6905	T6908	arg1Of	see,","
R5544	T6907	T6906	arg2Of	example,for
R5545	T6910	T6909	arg2Of	35,[
R5546	T6911	T6909	arg3Of	],[
R5547	T6912	T6905	arg2Of	–,see
R5548	T6912	T6909	arg1Of	–,[
R5549	T6912	T6913	arg1Of	–,[
R5550	T6914	T6913	arg2Of	37,[
R5551	T6915	T6913	arg3Of	],[
R5552	T6916	T6904	arg3Of	),(
R5553	T6918	T6917	arg2Of	stimulation,Upon
R5554	T6921	T6922	arg1Of	B,","
R5555	T6922	T6925	arg1Of	",",and
R5556	T6923	T6922	arg2Of	C,","
R5557	T6925	T6924	arg1Of	and,","
R5558	T6926	T6925	arg2Of	E,and
R5559	T6928	T6920	arg1Of	reporters,the
R5560	T6928	T6921	arg1Of	reporters,B
R5561	T6928	T6923	arg1Of	reporters,C
R5562	T6928	T6926	arg1Of	reporters,E
R5563	T6928	T6927	arg1Of	reporters,LTR-driven
R5564	T6928	T6929	arg1Of	reporters,demonstrated
R5565	T6929	T6940	arg1Of	demonstrated,and
R5566	T6932	T6929	arg2Of	enhancement,demonstrated
R5567	T6932	T6930	arg1Of	enhancement,a
R5568	T6932	T6931	arg1Of	enhancement,significant
R5569	T6932	T6933	arg1Of	enhancement,in
R5570	T6935	T6933	arg2Of	activity,in
R5571	T6935	T6934	arg1Of	activity,luciferase
R5572	T6935	T6936	arg1Of	activity,(
R5573	T6938	T6936	arg2Of	1A,(
R5574	T6938	T6937	arg1Of	1A,Figure
R5575	T6939	T6936	arg3Of	),(
R5576	T6940	T6917	arg1Of	and,Upon
R5577	T6940	T6919	arg1Of	and,","
R5578	T6942	T6941	arg1Of	magnitude,the
R5579	T6942	T6943	arg1Of	magnitude,of
R5580	T6942	T6946	arg1Of	magnitude,was
R5581	T6942	T6947	arg1Of	magnitude,subtype-specific
R5582	T6945	T6943	arg2Of	effect,of
R5583	T6945	T6944	arg1Of	effect,this
R5584	T6946	T6940	arg2Of	was,and
R5585	T6947	T6946	arg2Of	subtype-specific,was
R5586	T6950	T6948	arg1Of	LTRs,Subtype
R5587	T6950	T6949	arg1Of	LTRs,C
R5588	T6950	T6951	arg1Of	LTRs,displayed
R5589	T6951	T6955	arg1Of	displayed,","
R5590	T6951	T6956	arg1Of	displayed,while
R5591	T6954	T6951	arg2Of	activity,displayed
R5592	T6954	T6952	arg1Of	activity,the
R5593	T6954	T6953	arg1Of	activity,strongest
R5594	T6958	T6957	arg1Of	LTRs,the
R5595	T6958	T6959	arg1Of	LTRs,from
R5596	T6958	T6964	arg1Of	LTRs,showed
R5597	T6962	T6959	arg2Of	isolates,from
R5598	T6962	T6960	arg1Of	isolates,subtype
R5599	T6962	T6961	arg1Of	isolates,E
R5600	T6964	T6956	arg2Of	showed,while
R5601	T6964	T6963	arg1Of	showed,consistently
R5602	T6964	T6972	arg1Of	showed,","
R5603	T6964	T6973	arg1Of	showed,consistent
R5604	T6967	T6964	arg2Of	activity,showed
R5605	T6967	T6965	arg1Of	activity,the
R5606	T6967	T6966	arg1Of	activity,weakest
R5607	T6967	T6968	arg1Of	activity,(
R5608	T6970	T6968	arg2Of	1A,(
R5609	T6970	T6969	arg1Of	1A,Figure
R5610	T6971	T6968	arg3Of	),(
R5611	T6973	T6974	arg1Of	consistent,with
R5612	T6976	T6974	arg2Of	studies,with
R5613	T6976	T6975	arg1Of	studies,previous
R5614	T6976	T6977	arg1Of	studies,demonstrating
R5615	T6980	T6977	arg2Of	activity,demonstrating
R5616	T6980	T6978	arg1Of	activity,subtype-specific
R5617	T6980	T6979	arg1Of	activity,LTR
R5618	T6980	T6981	arg1Of	activity,that
R5619	T6980	T6982	arg1Of	activity,used
R5620	T6982	T6984	arg1Of	used,as
R5621	T6982	T6990	arg1Of	used,","
R5622	T6982	T6991	arg1Of	used,[
R5623	T6983	T6982	arg2Of	TNF,used
R5624	T6986	T6984	arg2Of	stimulus,as
R5625	T6986	T6985	arg1Of	stimulus,a
R5626	T6986	T6987	arg1Of	stimulus,[
R5627	T6988	T6987	arg2Of	38,[
R5628	T6989	T6987	arg3Of	],[
R5629	T6992	T6991	arg2Of	39,[
R5630	T6993	T6991	arg3Of	],[
R15471	T21124	T21123	arg1Of	interaction,NFAT5
R15472	T21124	T21125	arg1Of	interaction,with
R15473	T21124	T21128	arg1Of	interaction,is
R15474	T21124	T21129	arg1Of	interaction,important
R15475	T21127	T21125	arg2Of	LTR,with
R15476	T21127	T21126	arg1Of	LTR,the
R15477	T21129	T21128	arg2Of	important,is
R15478	T21129	T21130	arg1Of	important,for
R15479	T21133	T21130	arg2Of	transcription,for
R15480	T21133	T21131	arg1Of	transcription,MTb-induced
R15481	T21133	T21132	arg1Of	transcription,HIV-1
R15482	T21135	T21134	arg2Of	A,(
R15483	T21136	T21134	arg3Of	),(
R15484	T21138	T21134	arg1Of	stimulation,(
R15485	T21138	T21137	arg1Of	stimulation,MTb
R15486	T21138	T21139	arg1Of	stimulation,increases
R15487	T21139	T21174	arg1Of	increases,and
R15488	T21140	T21139	arg2Of	activity,increases
R15489	T21140	T21141	arg1Of	activity,of
R15490	T21142	T21143	arg2Of	LTRs,derived
R15491	T21142	T21171	arg1Of	LTRs,","
R15492	T21143	T21144	arg1Of	derived,from
R15493	T21146	T21145	arg1Of	subtypes,HIV-1
R15494	T21147	T21148	arg1Of	B,","
R15495	T21148	T21146	arg1Of	",",subtypes
R15496	T21148	T21151	arg1Of	",",and
R15497	T21149	T21148	arg2Of	C,","
R15498	T21151	T21144	arg2Of	and,from
R15499	T21151	T21150	arg1Of	and,","
R15500	T21154	T21151	arg2Of	LTRs,and
R15501	T21154	T21152	arg1Of	LTRs,E.
R15502	T21154	T21153	arg1Of	LTRs,HIV-1
R15503	T21154	T21155	arg1Of	LTRs,(
R15504	T21154	T21167	arg1Of	LTRs,from
R15505	T21156	T21157	arg1Of	−208,to
R15506	T21158	T21157	arg2Of	+64,to
R15507	T21159	T21155	arg2Of	nt,(
R15508	T21159	T21156	arg1Of	nt,−208
R15509	T21159	T21160	arg1Of	nt,relative
R15510	T21160	T21161	arg1Of	relative,to
R15511	T21165	T21161	arg2Of	site,to
R15512	T21165	T21162	arg1Of	site,the
R15513	T21165	T21163	arg1Of	site,transcription
R15514	T21165	T21164	arg1Of	site,start
R15515	T21166	T21155	arg3Of	),(
R15516	T21170	T21167	arg2Of	B,from
R15517	T21170	T21168	arg1Of	B,representative
R15518	T21170	T21169	arg1Of	B,subtype
R15519	T21171	T21141	arg2Of	",",of
R15520	T21172	T21171	arg2Of	C,","
R15521	T21174	T21173	arg1Of	and,","
R15522	T21177	T21175	arg1Of	isolates,E
R15523	T21177	T21176	arg1Of	isolates,viral
R15524	T21177	T21178	arg1Of	isolates,were
R15525	T21177	T21179	arg2Of	isolates,cloned
R15526	T21179	T21174	arg2Of	cloned,and
R15527	T21179	T21178	arg2Of	cloned,were
R15528	T21179	T21180	arg1Of	cloned,into
R15529	T21182	T21180	arg2Of	pGL3,into
R15530	T21182	T21181	arg1Of	pGL3,plasmid
R15531	T21184	T21183	arg1Of	cells,THP-1
R15532	T21184	T21185	arg1Of	cells,(
R15533	T21184	T21188	arg1Of	cells,were
R15534	T21184	T21189	arg2Of	cells,transfected
R15535	T21184	T21210	arg2Of	cells,incubated
R15536	T21186	T21185	arg2Of	0.8×106/ml,(
R15537	T21187	T21185	arg3Of	),(
R15538	T21189	T21190	arg1Of	transfected,with
R15539	T21189	T21209	arg1Of	transfected,and
R15540	T21193	T21191	arg1Of	plasmid,each
R15541	T21193	T21192	arg1Of	plasmid,reporter
R15542	T21193	T21194	arg1Of	plasmid,(
R15543	T21193	T21198	arg1Of	plasmid,plus
R15544	T21196	T21194	arg2Of	µg/ml,(
R15545	T21196	T21195	arg1Of	µg/ml,0.3
R15546	T21197	T21194	arg3Of	),(
R15547	T21198	T21190	arg2Of	plus,with
R15548	T21204	T21198	arg2Of	pRL-TK,plus
R15549	T21204	T21199	arg1Of	pRL-TK,the
R15550	T21204	T21200	arg1Of	pRL-TK,Renilla
R15551	T21204	T21201	arg1Of	pRL-TK,luciferase
R15552	T21204	T21202	arg1Of	pRL-TK,control
R15553	T21204	T21203	arg1Of	pRL-TK,plasmid
R15554	T21204	T21205	arg1Of	pRL-TK,(
R15555	T21207	T21205	arg2Of	µg/ml,(
R15556	T21207	T21206	arg1Of	µg/ml,0.03
R15557	T21208	T21205	arg3Of	),(
R15558	T21209	T21188	arg2Of	and,were
R15559	T21210	T21209	arg2Of	incubated,and
R15560	T21210	T21211	arg1Of	incubated,at
R15561	T21210	T21213	arg1Of	incubated,for
R15562	T21212	T21211	arg2Of	37°C,at
R15563	T21215	T21213	arg2Of	hours,for
R15564	T21215	T21214	arg1Of	hours,16
R15565	T21216	T21217	arg1Of	Cells,were
R15566	T21216	T21221	arg1Of	Cells,untreated
R15567	T21216	T21223	arg2Of	Cells,treated
R15568	T21217	T21218	arg1Of	were,then
R15569	T21221	T21220	arg1Of	untreated,left
R15570	T21221	T21222	arg1Of	untreated,or
R15571	T21222	T21217	arg2Of	or,were
R15572	T21222	T21219	arg1Of	or,either
R15573	T21223	T21222	arg2Of	treated,or
R15574	T21223	T21224	arg1Of	treated,with
R15575	T21223	T21232	arg1Of	treated,before
R15576	T21228	T21224	arg2Of	lysate,with
R15577	T21228	T21225	arg1Of	lysate,10
R15578	T21228	T21226	arg1Of	lysate,µg/ml
R15579	T21228	T21227	arg1Of	lysate,MTb
R15580	T21228	T21229	arg1Of	lysate,for
R15581	T21231	T21229	arg2Of	hours,for
R15582	T21231	T21230	arg1Of	hours,8
R15583	T21233	T21232	arg2Of	termination,before
R15584	T21233	T21234	arg1Of	termination,of
R15585	T21236	T21234	arg2Of	cultures,of
R15586	T21236	T21235	arg1Of	cultures,the
R15587	T21239	T21237	arg2Of	histogram,In
R15588	T21239	T21238	arg1Of	histogram,the
R15589	T21242	T21241	arg1Of	bars,open
R15590	T21242	T21243	arg1Of	bars,represent
R15591	T21243	T21237	arg1Of	represent,In
R15592	T21243	T21240	arg1Of	represent,","
R15593	T21243	T21247	arg1Of	represent,in
R15594	T21246	T21243	arg2Of	activities,represent
R15595	T21246	T21244	arg1Of	activities,individual
R15596	T21246	T21245	arg1Of	activities,LTR
R15597	T21249	T21247	arg2Of	cells,in
R15598	T21249	T21248	arg1Of	cells,untreated
R15599	T21252	T21250	arg1Of	bars,Light
R15600	T21252	T21251	arg1Of	bars,grey
R15601	T21252	T21253	arg1Of	bars,represent
R15602	T21255	T21253	arg2Of	values,represent
R15603	T21255	T21254	arg1Of	values,mean
R15604	T21255	T21256	arg1Of	values,of
R15605	T21258	T21256	arg2Of	activities,of
R15606	T21258	T21257	arg1Of	activities,LTR
R15607	T21258	T21259	arg1Of	activities,from
R15608	T21261	T21259	arg2Of	subtype,from
R15609	T21261	T21260	arg1Of	subtype,each
R15610	T21261	T21262	arg1Of	subtype,in
R15611	T21264	T21262	arg2Of	cells,in
R15612	T21264	T21263	arg1Of	cells,untreated
R15613	T21266	T21265	arg1Of	bars,Black
R15614	T21266	T21267	arg1Of	bars,represent
R15615	T21267	T21276	arg1Of	represent,and
R15616	T21270	T21267	arg2Of	activities,represent
R15617	T21270	T21268	arg1Of	activities,individual
R15618	T21270	T21269	arg1Of	activities,LTR
R15619	T21270	T21271	arg1Of	activities,in
R15620	T21274	T21271	arg2Of	cells,in
R15621	T21274	T21272	arg1Of	cells,MTb
R15622	T21274	T21273	arg1Of	cells,lysate-treated
R15623	T21276	T21275	arg1Of	and,","
R15624	T21279	T21277	arg1Of	bars,dark
R15625	T21279	T21278	arg1Of	bars,grey
R15626	T21279	T21280	arg1Of	bars,represent
R15627	T21280	T21276	arg2Of	represent,and
R15628	T21280	T21289	arg1Of	represent,in
R15629	T21282	T21280	arg2Of	values,represent
R15630	T21282	T21281	arg1Of	values,mean
R15631	T21282	T21283	arg1Of	values,of
R15632	T21285	T21283	arg2Of	activities,of
R15633	T21285	T21284	arg1Of	activities,LTR
R15634	T21285	T21286	arg1Of	activities,from
R15635	T21288	T21286	arg2Of	subtype,from
R15636	T21288	T21287	arg1Of	subtype,each
R15637	T21290	T21289	arg2Of	cells,in
R15638	T21290	T21291	arg2Of	cells,treated
R15639	T21291	T21292	arg1Of	treated,with
R15640	T21294	T21292	arg2Of	lysate,with
R15641	T21294	T21293	arg1Of	lysate,MTb
R15642	T21297	T21295	arg1Of	activity,LTR
R15643	T21297	T21296	arg1Of	activity,transcriptional
R15644	T21297	T21298	arg1Of	activity,for
R15645	T21297	T21305	arg1Of	activity,was
R15646	T21297	T21307	arg2Of	activity,increased
R15647	T21299	T21298	arg2Of	all,for
R15648	T21299	T21300	arg1Of	all,of
R15649	T21303	T21300	arg2Of	LTRs,of
R15650	T21303	T21301	arg1Of	LTRs,the
R15651	T21303	T21302	arg1Of	LTRs,representative
R15652	T21303	T21304	arg2Of	LTRs,tested
R15653	T21307	T21305	arg2Of	increased,was
R15654	T21307	T21306	arg1Of	increased,significantly
R15655	T21307	T21308	arg1Of	increased,in
R15656	T21309	T21308	arg2Of	cultures,in
R15657	T21309	T21310	arg2Of	cultures,treated
R15658	T21310	T21311	arg1Of	treated,with
R15659	T21310	T21314	arg1Of	treated,in
R15660	T21313	T21311	arg2Of	lysate,with
R15661	T21313	T21312	arg1Of	lysate,MTb
R15662	T21315	T21314	arg2Of	comparison,in
R15663	T21315	T21316	arg1Of	comparison,to
R15664	T21318	T21316	arg2Of	cultures,to
R15665	T21318	T21317	arg1Of	cultures,untreated
R15666	T21319	T21320	arg1Of	Results,are
R15667	T21324	T21321	arg2Of	experiments,from
R15668	T21324	T21322	arg1Of	experiments,three
R15669	T21324	T21323	arg1Of	experiments,independent
R15670	T21324	T21325	arg2Of	experiments,performed
R15671	T21324	T21328	arg1Of	experiments,(
R15672	T21325	T21326	arg1Of	performed,in
R15673	T21327	T21326	arg2Of	duplicate,in
R15674	T21329	T21330	arg1Of	*,","
R15675	T21331	T21330	arg2Of	p,","
R15676	T21332	T21335	arg1Of	<,**
R15677	T21333	T21334	arg1Of	0.05,;
R15678	T21335	T21333	arg1Of	**,0.05
R15679	T21335	T21336	arg1Of	**,","
R15680	T21337	T21336	arg2Of	p,","
R15681	T21337	T21338	arg1Of	p,<
R15682	T21339	T21328	arg2Of	0.01,(
R15683	T21339	T21329	arg1Of	0.01,*
R15684	T21339	T21332	arg1Of	0.01,<
R15685	T21339	T21340	arg1Of	0.01,as
R15686	T21341	T21340	arg2Of	compared,as
R15687	T21341	T21342	arg1Of	compared,to
R15688	T21344	T21342	arg2Of	cultures,to
R15689	T21344	T21343	arg1Of	cultures,unstimulated
R15690	T21345	T21328	arg3Of	),(
R15691	T21347	T21346	arg2Of	(,.
R15692	T21348	T21346	arg3Of	B,.
R15693	T21350	T21321	arg1Of	ic disrupt,from
R15694	T21350	T21346	arg1Of	ic disrupt,.
R15695	T21350	T21349	arg1Of	ic disrupt,) Specif
R15696	T21350	T21351	arg1Of	ic disrupt,io
R15697	T21350	T21357	arg1Of	ic disrupt,ficantl
R15698	T21355	T21351	arg2Of	indi,io
R15699	T21355	T21352	arg1Of	indi,n o
R15700	T21355	T21353	arg1Of	indi,f the
R15701	T21355	T21354	arg1Of	indi,NFAT5 b
R15702	T21357	T21320	arg2Of	ficantl,are
R15703	T21357	T21356	arg1Of	ficantl,ng site signi
R15704	T21357	T21364	arg1Of	ficantl,mo
R15705	T21360	T21357	arg2Of	porter g,ficantl
R15706	T21360	T21358	arg1Of	porter g,y reduces LT
R15707	T21360	T21359	arg1Of	porter g,R-re
R15708	T21360	T21361	arg1Of	porter g,en
R15709	T21363	T21361	arg2Of	y in ,en
R15710	T21363	T21362	arg1Of	y in ,e activit
R15711	T21365	T21364	arg2Of	nocytic ,mo
R15712	T21365	T21366	arg1Of	nocytic ,ce
R15713	T21369	T21366	arg2Of	ponse to ,ce
R15714	T21369	T21367	arg1Of	ponse to ,lls
R15715	T21369	T21368	arg1Of	ponse to ,in res
R15716	T21371	T21370	arg1Of	cells,THP-1
R15717	T21371	T21372	arg1Of	cells,were
R15718	T21371	T21373	arg2Of	cells,transfected
R15719	T21373	T21372	arg2Of	transfected,were
R15720	T21373	T21374	arg1Of	transfected,with
R15721	T21377	T21374	arg2Of	vectors,with
R15722	T21377	T21375	arg1Of	vectors,luciferase
R15723	T21377	T21376	arg1Of	vectors,expression
R15724	T21377	T21378	arg1Of	vectors,encoding
R15725	T21382	T21379	arg1Of	+64,nucleotides
R15726	T21382	T21380	arg1Of	+64,208
R15727	T21382	T21381	arg1Of	+64,to
R15728	T21382	T21383	arg1Of	+64,of
R15729	T21382	T21388	arg1Of	+64,and
R15730	T21387	T21383	arg2Of	LTR,of
R15731	T21387	T21384	arg1Of	LTR,the
R15732	T21387	T21385	arg1Of	LTR,wild-type
R15733	T21387	T21386	arg1Of	LTR,HIV-1Lai
R15734	T21388	T21378	arg2Of	and,encoding
R15735	T21391	T21388	arg2Of	LTR,and
R15736	T21391	T21389	arg1Of	LTR,an
R15737	T21391	T21390	arg1Of	LTR,HIV-1Lai
R15738	T21391	T21392	arg1Of	LTR,containing
R15739	T21397	T21392	arg2Of	mutations,containing
R15740	T21397	T21393	arg1Of	mutations,the
R15741	T21397	T21394	arg1Of	mutations,NFAT5
R15742	T21397	T21395	arg1Of	mutations,binding
R15743	T21397	T21396	arg1Of	mutations,site
R15744	T21397	T21398	arg1Of	mutations,(
R15745	T21399	T21398	arg2Of	N5-Mut,(
R15746	T21400	T21398	arg3Of	),(
R15747	T21403	T21401	arg2Of	hours,After
R15748	T21403	T21402	arg1Of	hours,16
R15749	T21406	T21405	arg1Of	cells,the
R15750	T21406	T21407	arg1Of	cells,were
R15751	T21406	T21408	arg2Of	cells,left
R15752	T21406	T21409	arg1Of	cells,untreated
R15753	T21406	T21411	arg2Of	cells,exposed
R15754	T21408	T21410	arg1Of	left,or
R15755	T21409	T21408	arg3Of	untreated,left
R15756	T21410	T21401	arg1Of	or,After
R15757	T21410	T21404	arg1Of	or,","
R15758	T21410	T21407	arg2Of	or,were
R15759	T21411	T21410	arg2Of	exposed,or
R15760	T21411	T21412	arg1Of	exposed,to
R15761	T21411	T21417	arg1Of	exposed,for
R15762	T21411	T21431	arg3Of	exposed,suppressed
R15763	T21416	T21412	arg2Of	lysate,to
R15764	T21416	T21413	arg1Of	lysate,10
R15765	T21416	T21414	arg1Of	lysate,µg/ml
R15766	T21416	T21415	arg1Of	lysate,MTb
R15767	T21419	T21417	arg2Of	hours,for
R15768	T21419	T21418	arg1Of	hours,8
R15769	T21419	T21420	arg1Of	hours,at
R15770	T21422	T21420	arg2Of	Disruption,at
R15771	T21422	T21421	arg1Of	Disruption,37°C.
R15772	T21422	T21423	arg1Of	Disruption,of
R15773	T21424	T21423	arg2Of	NFAT5,of
R15774	T21425	T21426	arg1Of	binding,to
R15775	T21425	T21431	arg1Of	binding,suppressed
R15776	T21429	T21426	arg2Of	region,to
R15777	T21429	T21427	arg1Of	region,the
R15778	T21429	T21428	arg1Of	region,enhancer
R15779	T21431	T21430	arg1Of	suppressed,significantly
R15780	T21431	T21436	arg1Of	suppressed,in
R15781	T21431	T21442	arg1Of	suppressed,when
R15782	T21435	T21431	arg2Of	expression,suppressed
R15783	T21435	T21432	arg1Of	expression,LTR-driven
R15784	T21435	T21433	arg1Of	expression,reporter
R15785	T21435	T21434	arg1Of	expression,gene
R15786	T21436	T21438	arg1Of	in,to
R15787	T21437	T21436	arg2Of	comparison,in
R15788	T21441	T21436	arg3Of	LTR,in
R15789	T21441	T21439	arg1Of	LTR,the
R15790	T21441	T21440	arg1Of	LTR,wild-type
R15791	T21443	T21444	arg1Of	cells,were
R15792	T21443	T21445	arg2Of	cells,treated
R15793	T21445	T21442	arg2Of	treated,when
R15794	T21445	T21444	arg2Of	treated,were
R15795	T21445	T21446	arg1Of	treated,with
R15796	T21448	T21446	arg2Of	lysate,with
R15797	T21448	T21447	arg1Of	lysate,MTb
R15798	T21448	T21449	arg1Of	lysate,(
R15799	T21450	T21449	arg2Of	p,(
R15800	T21450	T21451	arg1Of	p,<
R15801	T21450	T21452	arg1Of	p,0.01
R15802	T21453	T21449	arg3Of	),(
R15803	T21455	T21454	arg1Of	activity,LTR
R15804	T21455	T21456	arg1Of	activity,was
R15805	T21455	T21458	arg2Of	activity,suppressed
R15806	T21458	T21456	arg2Of	suppressed,was
R15807	T21458	T21457	arg1Of	suppressed,also
R15808	T21458	T21459	arg1Of	suppressed,in
R15809	T21458	T21464	arg1Of	suppressed,to
R15810	T21459	T21463	arg1Of	in,but
R15811	T21462	T21459	arg2Of	cells,in
R15812	T21462	T21460	arg1Of	cells,the
R15813	T21462	T21461	arg1Of	cells,untreated
R15814	T21464	T21463	arg2Of	to,but
R15815	T21467	T21464	arg2Of	extent,to
R15816	T21467	T21465	arg1Of	extent,a
R15817	T21467	T21466	arg1Of	extent,lesser
R15818	T21467	T21468	arg1Of	extent,(
R15819	T21469	T21468	arg2Of	p,(
R15820	T21469	T21470	arg1Of	p,<
R15821	T21469	T21471	arg1Of	p,0.05
R15822	T21472	T21468	arg3Of	),(
R15823	T21473	T21474	arg1Of	Results,are
R15824	T21473	T21475	arg1Of	Results,from
R15825	T21475	T21474	arg2Of	from,are
R15826	T21478	T21475	arg2Of	experiments,from
R15827	T21478	T21476	arg1Of	experiments,three
R15828	T21478	T21477	arg1Of	experiments,independent
R15829	T21478	T21479	arg2Of	experiments,performed
R15830	T21478	T21483	arg2Of	experiments,adjusted
R15831	T21479	T21480	arg1Of	performed,in
R15832	T21479	T21482	arg1Of	performed,and
R15833	T21481	T21480	arg2Of	duplicate,in
R15834	T21483	T21482	arg2Of	adjusted,and
R15835	T21483	T21484	arg1Of	adjusted,to
R15836	T21488	T21484	arg2Of	expression,to
R15837	T21488	T21485	arg1Of	expression,Renilla
R15838	T21488	T21486	arg1Of	expression,luciferase
R15839	T21488	T21487	arg1Of	expression,control
R15840	T21488	T21489	arg1Of	expression,(
R15841	T21490	T21491	arg1Of	*,","
R15842	T21492	T21491	arg2Of	p,","
R15843	T21494	T21489	arg2Of	0.05,(
R15844	T21494	T21490	arg1Of	0.05,*
R15845	T21494	T21493	arg1Of	0.05,<
R15846	T21494	T21495	arg1Of	0.05,;
R15847	T21496	T21495	arg2Of	**,;
R15848	T21496	T21497	arg1Of	**,","
R15849	T21498	T21497	arg2Of	p,","
R15850	T21498	T21499	arg1Of	p,<
R15851	T21498	T21500	arg1Of	p,0.01
R15852	T21501	T21489	arg3Of	),(
R15853	T21503	T21502	arg1Of	sequences,Nucleotide
R15854	T21503	T21504	arg1Of	sequences,representing
R15855	T21503	T21513	arg1Of	sequences,are
R15856	T21503	T21514	arg2Of	sequences,shown
R15857	T21506	T21507	arg1Of	wild-type,and
R15858	T21507	T21504	arg2Of	and,representing
R15859	T21507	T21505	arg1Of	and,the
R15860	T21512	T21507	arg2Of	LTRs,and
R15861	T21512	T21508	arg1Of	LTRs,NFAT5
R15862	T21512	T21509	arg1Of	LTRs,binding
R15863	T21512	T21510	arg1Of	LTRs,site-mutated
R15864	T21512	T21511	arg1Of	LTRs,HIV-1Lai
R15865	T21514	T21513	arg2Of	shown,are
R15866	T21514	T21515	arg1Of	shown,at
R15867	T21517	T21515	arg2Of	bottom,at
R15868	T21517	T21516	arg1Of	bottom,the
R15869	T21517	T21518	arg1Of	bottom,of
R15870	T21520	T21518	arg2Of	figure.,of
R15871	T21520	T21519	arg1Of	figure.,the
R15872	T21522	T21521	arg2Of	C,(
R15873	T21523	T21521	arg3Of	),(
R15874	T21525	T21521	arg1Of	lysate,(
R15875	T21525	T21524	arg1Of	lysate,MTb
R15876	T21525	T21526	arg1Of	lysate,increases
R15877	T21526	T21513	modOf	increases,are
R15878	T21529	T21526	arg2Of	levels,increases
R15879	T21529	T21527	arg1Of	levels,NFAT5
R15880	T21529	T21528	arg1Of	levels,protein
R15881	T21529	T21530	arg1Of	levels,in
R15882	T21532	T21530	arg2Of	cells,in
R15883	T21532	T21531	arg1Of	cells,monocytic
R15884	T21534	T21533	arg1Of	cells,THP-1
R15885	T21534	T21535	arg1Of	cells,were
R15886	T21534	T21536	arg2Of	cells,left
R15887	T21534	T21537	arg1Of	cells,untreated
R15888	T21534	T21542	arg2Of	cells,exposed
R15889	T21536	T21541	arg1Of	left,or
R15890	T21537	T21536	arg3Of	untreated,left
R15891	T21537	T21538	arg1Of	untreated,(
R15892	T21539	T21538	arg2Of	control,(
R15893	T21540	T21538	arg3Of	),(
R15894	T21541	T21535	arg2Of	or,were
R15895	T21541	T21552	arg1Of	or,hours
R15896	T21542	T21541	arg2Of	exposed,or
R15897	T21542	T21543	arg1Of	exposed,to
R15898	T21547	T21543	arg2Of	lysate,to
R15899	T21547	T21544	arg1Of	lysate,10
R15900	T21547	T21545	arg1Of	lysate,µg/ml
R15901	T21547	T21546	arg1Of	lysate,MTb
R15902	T21549	T21550	arg1Of	8,or
R15903	T21551	T21550	arg2Of	24,or
R15904	T21552	T21548	arg1Of	hours,for
R15905	T21552	T21549	arg1Of	hours,8
R15906	T21552	T21551	arg1Of	hours,24
R15907	T21552	T21553	arg1Of	hours,at
R15908	T21552	T21558	modOf	hours,were
R15909	T21555	T21553	arg2Of	Whole,at
R15910	T21555	T21554	arg1Of	Whole,37°C.
R15911	T21557	T21556	arg1Of	extracts,cell
R15912	T21557	T21558	arg1Of	extracts,were
R15913	T21557	T21559	arg2Of	extracts,collected
R15914	T21557	T21561	arg2Of	extracts,analyzed
R15915	T21559	T21560	arg1Of	collected,and
R15916	T21560	T21558	arg2Of	and,were
R15917	T21561	T21560	arg2Of	analyzed,and
R15918	T21564	T21559	arg1Of	blot,collected
R15919	T21564	T21561	arg1Of	blot,analyzed
R15920	T21564	T21562	arg2Of	blot,by
R15921	T21564	T21563	arg1Of	blot,western
R15922	T21564	T21565	arg1Of	blot,with
R38	T209	T208	arg2Of	activating,by
R39	T210	T211	arg1Of	innate,and
R40	T212	T211	arg2Of	adaptive,and
R41	T215	T209	arg2Of	cascades,activating
R42	T215	T210	arg1Of	cascades,innate
R43	T215	T212	arg1Of	cascades,adaptive
R44	T215	T213	arg1Of	cascades,immune
R471	T183	T179	arg1Of	Transcription,Regulation
R472	T183	T180	arg1Of	Transcription,of Mycobacterium
R473	T183	T181	arg1Of	Transcription,tuberculosis-Dependent
R474	T183	T182	arg1Of	Transcription,HIV-1
R475	T183	T184	arg1Of	Transcription,Reveals
R476	T184	T190	arg1Of	Reveals,in
R477	T187	T184	arg2Of	Role,Reveals
R478	T187	T185	arg1Of	Role,a
R479	T187	T186	arg1Of	Role,New
R480	T187	T188	arg1Of	Role,for
R481	T189	T188	arg2Of	NFAT5,for
R482	T194	T190	arg2Of	Pathway,in
R483	T194	T191	arg1Of	Pathway,the
R484	T194	T192	arg1Of	Pathway,Toll-Like
R485	T194	T193	arg1Of	Pathway,Receptor
R486	T195	T196	arg1Of	Tuberculosis,(
R487	T197	T196	arg2Of	TB,(
R488	T198	T196	arg3Of	),(
R489	T199	T195	arg1Of	disease,Tuberculosis
R490	T199	T200	arg1Of	disease,in
R491	T199	T204	arg1Of	disease,contributes
R492	T199	T209	arg1Of	disease,activating
R493	T203	T200	arg2Of	patients,in
R494	T203	T201	arg1Of	patients,HIV
R495	T203	T202	arg1Of	patients,co-infected
R496	T204	T205	arg1Of	contributes,to
R497	T204	T208	arg1Of	contributes,by
R498	T207	T205	arg2Of	mortality,to
R499	T207	T206	arg2Of	mortality,increased
R4771	T6017	T6016	arg1Of	blot,Western
R4772	T6020	T6018	arg1Of	extracts,Whole
R4773	T6020	T6019	arg1Of	extracts,cell
R4774	T6020	T6021	arg1Of	extracts,were
R4775	T6020	T6022	arg2Of	extracts,collected
R4776	T6022	T6021	arg2Of	collected,were
R4777	T6022	T6023	arg1Of	collected,with
R4778	T6025	T6024	arg1Of	buffer,lysis
R4779	T6025	T6026	arg1Of	buffer,containing
R4781	T6027	T6028	arg1Of	150,mM
R4782	T6029	T6027	arg1Of	NaCl,150
R4783	T6029	T6030	arg1Of	NaCl,","
R4784	T6030	T6034	arg1Of	",",","
R4785	T6031	T6032	arg1Of	50,mM
R4786	T6033	T6030	arg2Of	Tris–HCl,","
R4787	T6033	T6031	arg1Of	Tris–HCl,50
R4788	T6034	T6026	arg2Of	",",containing
R4789	T6035	T6034	arg2Of	pH,","
R4790	T6035	T6036	arg1Of	pH,7.5
R4791	T6037	T6023	arg2Of	",",with
R5264	T6568	T6574	arg1Of	95°C,for
R5269	T6576	T6574	arg2Of	min,for
R5270	T6576	T6575	arg1Of	min,1
R5271	T6578	T6577	arg1Of	results,The
R5272	T6578	T6579	arg1Of	results,were
R5273	T6578	T6580	arg2Of	results,normalized
R5274	T6578	T6589	arg2Of	results,expressed
R5275	T6580	T6588	arg1Of	normalized,and
R5276	T6581	T6580	arg3Of	using,normalized
R5277	T6581	T6584	arg1Of	using,as
R5278	T6583	T6581	arg2Of	mRNA,using
R5279	T6583	T6582	arg1Of	mRNA,β-actin
R5280	T6587	T6584	arg2Of	control,as
R5281	T6587	T6585	arg1Of	control,an
R5282	T6587	T6586	arg1Of	control,internal
R5283	T6588	T6579	arg2Of	and,were
R5284	T6589	T6588	arg2Of	expressed,and
R5285	T6589	T6590	arg1Of	expressed,as
R3994	T5117	T5133	arg2Of	pLKO.1,validated
R5357	T6681	T6680	arg1Of	analysis,Statistical
R5358	T6685	T6686	arg1Of	results,are
R5359	T6685	T6687	arg2Of	results,expressed
R5360	T6687	T6682	arg1Of	expressed,Where
R5361	T6687	T6683	arg1Of	expressed,applicable
R5362	T6687	T6684	arg1Of	expressed,","
R5363	T6687	T6686	arg2Of	expressed,are
R5364	T6687	T6688	arg1Of	expressed,as
R5365	T6691	T6688	arg2Of	SEM,as
R5366	T6691	T6689	arg1Of	SEM,mean
R5367	T6691	T6690	arg1Of	SEM,±
R5368	T6692	T6693	arg1Of	Comparison,between
R5369	T6692	T6696	arg1Of	Comparison,was
R5370	T6692	T6697	arg2Of	Comparison,performed
R5371	T6695	T6693	arg2Of	groups,between
R5372	T6695	T6694	arg1Of	groups,two
R5373	T6697	T6696	arg2Of	performed,was
R5374	T6698	T6697	arg3Of	using,performed
R5375	T6698	T6703	arg1Of	using,with
R5376	T6702	T6698	arg2Of	t-Test,using
R5377	T6702	T6699	arg1Of	t-Test,the
R5378	T6702	T6700	arg1Of	t-Test,paired
R5379	T6702	T6701	arg1Of	t-Test,Student
R5380	T6705	T6703	arg2Of	aid,with

bionlp-st-ge-2016-test-proteins

Id	Subject	Object	Predicate	Lexical cue
T6778	16397-16400	Protein	denotes	TNF
T6777	16081-16091	Protein	denotes	luciferase
T6776	15660-15670	Protein	denotes	luciferase
T6534	15033-15040	Protein	denotes	β-actin
T6004	14428-14431	Protein	denotes	BSA
T6003	14105-14108	Protein	denotes	BSA
T2919	7305-7309	Protein	denotes	CD14
T2918	7209-7213	Protein	denotes	MCSF
T2917	6988-6992	Protein	denotes	MCSF
T2916	6735-6739	Protein	denotes	CD14
T997	6003-6008	Protein	denotes	NFAT5
T996	5921-5926	Protein	denotes	NFAT5
T995	5849-5854	Protein	denotes	MyD88
T994	5803-5808	Protein	denotes	NFAT5
T85	94-99	Protein	denotes	NFAT5
T4329	9983-9987	Protein	denotes	CCR5
T4328	9893-9898	Protein	denotes	CXCR4
T4327	9826-9831	Protein	denotes	gp160
T3598	9061-9071	Protein	denotes	luciferase
T3597	9012-9025	Protein	denotes	Reporter gene
T3596	8772-8782	Protein	denotes	luciferase
T86	497-534	Protein	denotes	nuclear factor of activated T cells 5
T87	536-541	Protein	denotes	NFAT5
T88	682-687	Protein	denotes	NFAT5
T89	1106-1111	Protein	denotes	MyD88
T90	1113-1118	Protein	denotes	IRAK1
T91	1124-1129	Protein	denotes	TRAF6
T92	1166-1171	Protein	denotes	NFAT5
T93	1247-1252	Protein	denotes	NFAT5
T94	1286-1291	Protein	denotes	NFAT5
T95	1453-1458	Protein	denotes	NFAT5
T993	5606-5611	Protein	denotes	NFAT5
T992	5426-5431	Protein	denotes	NFAT5
T991	5384-5389	Protein	denotes	MyD88
T990	5272-5277	Protein	denotes	NFAT5
T989	4896-4902	Protein	denotes	TonEBP
T988	4875-4880	Protein	denotes	NFAT5
T987	3893-3898	Protein	denotes	TRAF6
T986	3850-3855	Protein	denotes	TRAF6
T985	3818-3823	Protein	denotes	IRAK4
T984	3808-3813	Protein	denotes	IRAK1
T983	3741-3746	Protein	denotes	MyD88
T982	3691-3695	Protein	denotes	TLR2
T981	3639-3665	Protein	denotes	toll-like receptor (TLR) 2
T980	3284-3287	Protein	denotes	CD4
T979	3053-3056	Protein	denotes	CD4
T5095	11801-11806	Protein	denotes	TRAF6
T5094	11638-11643	Protein	denotes	TRAF6
T5093	11586-11591	Protein	denotes	IRAK1
T5092	11418-11423	Protein	denotes	IRAK1
T5091	11293-11298	Protein	denotes	MyD88
T5090	11271-11276	Protein	denotes	shRNA
T5089	11224-11229	Protein	denotes	shRNA
T4094	9492-9499	Protein	denotes	DNase I
T4093	9423-9426	Protein	denotes	p65
T4092	9415-9418	Protein	denotes	p50
T4091	9203-9208	Protein	denotes	NFAT5
T4090	9170-9177	Protein	denotes	DNase I
T4664	11126-11131	Protein	denotes	NFAT5
T4663	10650-10653	Protein	denotes	GFP
T4662	10623-10648	Protein	denotes	green fluorescent protein
T4661	10470-10475	Protein	denotes	NFAT5
T21076	18815-18820	Protein	denotes	NFAT5
T21075	18603-18613	Protein	denotes	luciferase
T21074	18237-18242	Protein	denotes	NFAT5
T21073	17956-17966	Protein	denotes	luciferase
T21072	16893-16903	Protein	denotes	luciferase
T21071	16471-16476	Protein	denotes	NFAT5

bionlp-st-ge-2016-uniprot

Id	Subject	Object	Predicate	Lexical cue
T21622	18603-18613	http://www.uniprot.org/uniprot/P08659	denotes	luciferase
T21621	17956-17966	http://www.uniprot.org/uniprot/P08659	denotes	luciferase
T21620	16893-16903	http://www.uniprot.org/uniprot/P08659	denotes	luciferase
T21618	19033-19038	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T21617	18815-18820	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T21616	18710-18715	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T21615	18237-18242	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T21614	18083-18088	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T21613	17798-17803	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T21612	16471-16476	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T6996	16397-16400	http://www.uniprot.org/uniprot/P01375	denotes	TNF
T6995	16081-16091	http://www.uniprot.org/uniprot/P08659	denotes	luciferase
T6994	15660-15670	http://www.uniprot.org/uniprot/P08659	denotes	luciferase
T5296	11801-11806	http://www.uniprot.org/uniprot/Q9Y4K3	denotes	TRAF6
T5295	11638-11643	http://www.uniprot.org/uniprot/Q9Y4K3	denotes	TRAF6
T5294	11293-11298	http://www.uniprot.org/uniprot/Q99836	denotes	MyD88
T4817	10650-10653	http://www.uniprot.org/uniprot/Q9U6Y4	denotes	GFP
T4816	11126-11131	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T4815	10470-10475	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T4453	10031-10035	http://www.uniprot.org/uniprot/P51681	denotes	CCR5
T4452	9983-9987	http://www.uniprot.org/uniprot/P51681	denotes	CCR5
T4451	9821-9824	http://www.uniprot.org/uniprot/P04578	denotes	env
T3800	9061-9071	http://www.uniprot.org/uniprot/P08659	denotes	luciferase
T3799	8772-8782	http://www.uniprot.org/uniprot/P08659	denotes	luciferase
T1794	5345-5359	http://www.uniprot.org/uniprot/Q15759	denotes	MAP kinase p38
T1793	5345-5359	http://www.uniprot.org/uniprot/P53778	denotes	MAP kinase p38
T1792	5345-5359	http://www.uniprot.org/uniprot/O15264	denotes	MAP kinase p38
T1791	5345-5359	http://www.uniprot.org/uniprot/Q16539	denotes	MAP kinase p38
T1790	4896-4902	http://www.uniprot.org/uniprot/O94916	denotes	TonEBP
T1789	5921-5926	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T1788	5803-5808	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T1787	5606-5611	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T1786	5426-5431	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T1785	5272-5277	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T1784	5044-5049	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T1783	4875-4880	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T1782	4081-4085	http://www.uniprot.org/uniprot/P05412	denotes	AP-1
T1781	3909-3919	http://www.uniprot.org/uniprot/Q60680-2	denotes	IκB kinase
T1780	3909-3919	http://www.uniprot.org/uniprot/O15111	denotes	IκB kinase
T1779	3893-3898	http://www.uniprot.org/uniprot/Q9Y4K3	denotes	TRAF6
T1778	3850-3855	http://www.uniprot.org/uniprot/Q9Y4K3	denotes	TRAF6
T1777	5849-5854	http://www.uniprot.org/uniprot/Q99836	denotes	MyD88
T1776	5384-5389	http://www.uniprot.org/uniprot/Q99836	denotes	MyD88
T1775	3741-3746	http://www.uniprot.org/uniprot/Q99836	denotes	MyD88
T1774	3691-3695	http://www.uniprot.org/uniprot/O60603	denotes	TLR2
T1773	3284-3287	http://www.uniprot.org/uniprot/P01730	denotes	CD4
T1772	3053-3056	http://www.uniprot.org/uniprot/P01730	denotes	CD4
T3798	8516-8521	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T412	536-541	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T413	682-687	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T414	808-813	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T415	1166-1171	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T416	1247-1252	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T417	1286-1291	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T418	1453-1458	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T419	1106-1111	http://www.uniprot.org/uniprot/Q99836	denotes	MyD88
T420	1124-1129	http://www.uniprot.org/uniprot/Q9Y4K3	denotes	TRAF6
T411	94-99	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T4176	9423-9426	http://www.uniprot.org/uniprot/P21579	denotes	p65
T4175	9423-9426	http://www.uniprot.org/uniprot/Q04206	denotes	p65
T4174	9415-9418	http://www.uniprot.org/uniprot/P19838	denotes	p50
T4173	9203-9208	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T3167	6988-6992	http://www.uniprot.org/uniprot/P09603	denotes	MCSF
T3166	7305-7309	http://www.uniprot.org/uniprot/P08571	denotes	CD14
T3165	6735-6739	http://www.uniprot.org/uniprot/P08571	denotes	CD14
T6593	15033-15040	http://www.uniprot.org/uniprot/P60709	denotes	β-actin
T6235	14287-14292	http://www.uniprot.org/uniprot/O94916	denotes	NFAT5
T3168	7209-7213	http://www.uniprot.org/uniprot/P09603	denotes	MCSF

UBERON-AE

Id	Subject	Object	Predicate	Lexical cue
T4645	10958-10963	http://purl.obolibrary.org/obo/UBERON_0001977	denotes	serum
T2903	7052-7057	http://purl.obolibrary.org/obo/UBERON_0001977	denotes	serum
T2902	6604-6609	http://purl.obolibrary.org/obo/UBERON_0001977	denotes	serum
T3465	7788-7799	http://purl.obolibrary.org/obo/UBERON_0012131	denotes	Centralized
T2809	6258-6263	http://purl.obolibrary.org/obo/UBERON_0000178	denotes	Blood
T2808	6199-6204	http://purl.obolibrary.org/obo/UBERON_0000178	denotes	blood
T2807	6175-6180	http://purl.obolibrary.org/obo/UBERON_0000178	denotes	blood
T924	5555-5560	http://purl.obolibrary.org/obo/UBERON_0000178	denotes	blood

GO-BP

Id	Subject	Object	Predicate	Lexical cue
T96	0-10	http://purl.obolibrary.org/obo/GO_0065007	denotes	Regulation
T98	57-70	http://purl.obolibrary.org/obo/GO_0006351	denotes	Transcription
T21082	18319-18334	http://purl.obolibrary.org/obo/GO_0010467	denotes	gene expression
T21081	17467-17482	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcriptional
T21080	16683-16696	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcription
T21079	16537-16550	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcription
T1012	3012-3040	http://purl.obolibrary.org/obo/GO_1904515	denotes	activate signal transduction
T1011	2978-2994	http://purl.obolibrary.org/obo/GO_0075136	denotes	host in response
T1010	5310-5327	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcriptionally
T1009	4167-4180	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcription
T1008	4026-4039	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcription
T1007	2850-2863	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcription
T1006	3021-3040	http://purl.obolibrary.org/obo/GO_0007165	denotes	signal transduction
T1005	2810-2828	http://purl.obolibrary.org/obo/GO_0007165	denotes	signaling cascades
T1004	5390-5399	http://purl.obolibrary.org/obo/GO_0023052	denotes	signaling
T1003	2810-2819	http://purl.obolibrary.org/obo/GO_0023052	denotes	signaling
T1002	3012-3040	http://purl.obolibrary.org/obo/GO_1903572	denotes	activate signal transduction
T1001	2800-2828	http://purl.obolibrary.org/obo/GO_1903572	denotes	activates signaling cascades
T1000	3012-3049	http://purl.obolibrary.org/obo/GO_1902533	denotes	activate signal transduction pathways
T999	2800-2828	http://purl.obolibrary.org/obo/GO_1902533	denotes	activates signaling cascades
T998	2622-2627	http://purl.obolibrary.org/obo/GO_0016265	denotes	death
T1016	3012-3049	http://purl.obolibrary.org/obo/GO_0038007	denotes	activate signal transduction pathways
T1015	3012-3040	http://purl.obolibrary.org/obo/GO_1902849	denotes	activate signal transduction
T1014	3012-3040	http://purl.obolibrary.org/obo/GO_0009967	denotes	activate signal transduction
T1013	3012-3040	http://purl.obolibrary.org/obo/GO_1904263	denotes	activate signal transduction
T111	1199-1221	http://purl.obolibrary.org/obo/GO_0002218	denotes	innate immune response
T110	1199-1221	http://purl.obolibrary.org/obo/GO_0045088	denotes	innate immune response
T109	1199-1221	http://purl.obolibrary.org/obo/GO_0002227	denotes	innate immune response
T108	1199-1221	http://purl.obolibrary.org/obo/GO_0045087	denotes	innate immune response
T107	1495-1510	http://purl.obolibrary.org/obo/GO_0010467	denotes	gene expression
T106	1172-1187	http://purl.obolibrary.org/obo/GO_0010467	denotes	gene expression
T105	999-1003	http://purl.obolibrary.org/obo/GO_0016246	denotes	RNAi
T104	564-568	http://purl.obolibrary.org/obo/GO_0016246	denotes	RNAi
T103	546-562	http://purl.obolibrary.org/obo/GO_0016246	denotes	RNA interference
T102	324-342	http://purl.obolibrary.org/obo/GO_0007165	denotes	signaling cascades
T101	1086-1095	http://purl.obolibrary.org/obo/GO_0023052	denotes	signaling
T100	324-333	http://purl.obolibrary.org/obo/GO_0023052	denotes	signaling
T99	476-489	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcription
T97	1320-1330	http://purl.obolibrary.org/obo/GO_0065007	denotes	regulation
T114	1206-1221	http://purl.obolibrary.org/obo/GO_0006955	denotes	immune response
T113	1199-1221	http://purl.obolibrary.org/obo/GO_0045824	denotes	innate immune response
T112	1199-1221	http://purl.obolibrary.org/obo/GO_0045089	denotes	innate immune response
T6785	16081-16100	http://purl.obolibrary.org/obo/GO_0050397	denotes	luciferase activity
T6784	16081-16100	http://purl.obolibrary.org/obo/GO_0050248	denotes	luciferase activity
T6783	16081-16100	http://purl.obolibrary.org/obo/GO_0047712	denotes	luciferase activity
T6782	16081-16100	http://purl.obolibrary.org/obo/GO_0047077	denotes	luciferase activity
T6781	16081-16100	http://purl.obolibrary.org/obo/GO_0045289	denotes	luciferase activity
T6780	15866-15888	http://purl.obolibrary.org/obo/GO_0006954	denotes	inflammatory responses
T6779	15612-15625	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcription
T6005	13652-13657	http://purl.obolibrary.org/obo/GO_0019835	denotes	lysis
T5606	13009-13015	http://purl.obolibrary.org/obo/GO_0040007	denotes	growth
T3600	9021-9036	http://purl.obolibrary.org/obo/GO_0010467	denotes	gene expression
T3599	8112-8127	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcriptional
T4330	9893-9898	http://purl.obolibrary.org/obo/GO_0038147	denotes	CXCR4
T1034	5965-5980	http://purl.obolibrary.org/obo/GO_0006955	denotes	immune response
T1033	5958-5980	http://purl.obolibrary.org/obo/GO_0045824	denotes	innate immune response
T1032	5958-5980	http://purl.obolibrary.org/obo/GO_0045089	denotes	innate immune response
T1031	5958-5980	http://purl.obolibrary.org/obo/GO_0002218	denotes	innate immune response
T1030	5958-5980	http://purl.obolibrary.org/obo/GO_0045088	denotes	innate immune response
T1029	5958-5980	http://purl.obolibrary.org/obo/GO_0002227	denotes	innate immune response
T1028	5958-5980	http://purl.obolibrary.org/obo/GO_0045087	denotes	innate immune response
T1027	5809-5824	http://purl.obolibrary.org/obo/GO_0010467	denotes	gene expression
T1026	5356-5359	http://purl.obolibrary.org/obo/GO_0004707	denotes	p38
T1025	5328-5355	http://purl.obolibrary.org/obo/GO_0000187	denotes	activated by the MAP kinase
T1024	4346-4363	http://purl.obolibrary.org/obo/GO_0051099	denotes	activator binding
T1023	4003-4039	http://purl.obolibrary.org/obo/GO_0045893	denotes	activation of specific transcription
T1022	3921-3924	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T1021	3028-3040	http://purl.obolibrary.org/obo/GO_0009293	denotes	transduction
T1020	3021-3049	http://purl.obolibrary.org/obo/GO_0035556	denotes	signal transduction pathways
T1019	3021-3049	http://purl.obolibrary.org/obo/GO_0036499	denotes	signal transduction pathways
T1018	3021-3049	http://purl.obolibrary.org/obo/GO_0036500	denotes	signal transduction pathways
T1017	3021-3049	http://purl.obolibrary.org/obo/GO_0036498	denotes	signal transduction pathways

GO-MF

Id	Subject	Object	Predicate	Lexical cue
T21087	18243-18266	http://purl.obolibrary.org/obo/GO_0035326	denotes	binding to the enhancer
T21086	18716-18723	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T21085	18243-18250	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T21084	18089-18096	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T21083	17804-17811	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T6786	16081-16100	http://purl.obolibrary.org/obo/GO_0045289	denotes	luciferase activity
T117	781-787	http://purl.obolibrary.org/obo/GO_0005488	denotes	ligand
T118	814-821	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T6790	16081-16100	http://purl.obolibrary.org/obo/GO_0050397	denotes	luciferase activity
T6789	16081-16100	http://purl.obolibrary.org/obo/GO_0050248	denotes	luciferase activity
T6788	16081-16100	http://purl.obolibrary.org/obo/GO_0047712	denotes	luciferase activity
T6787	16081-16100	http://purl.obolibrary.org/obo/GO_0047077	denotes	luciferase activity
T6007	14380-14388	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T6006	14293-14301	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T3601	8522-8529	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T1042	5079-5086	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T1041	4720-4727	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T1040	4356-4363	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding
T1039	3921-3924	http://purl.obolibrary.org/obo/GO_0008384	denotes	IKK
T1038	3833-3842	http://purl.obolibrary.org/obo/GO_0031386	denotes	ubiquitin
T4331	9893-9898	http://purl.obolibrary.org/obo/GO_0038147	denotes	CXCR4
T1046	5356-5359	http://purl.obolibrary.org/obo/GO_0004707	denotes	p38
T1045	5073-5086	http://purl.obolibrary.org/obo/GO_0051059	denotes	NF-κB binding
T1044	4714-4727	http://purl.obolibrary.org/obo/GO_0051059	denotes	NF-κB binding
T1043	5628-5635	http://purl.obolibrary.org/obo/GO_0005488	denotes	binding

GO-CC

Id	Subject	Object	Predicate	Lexical cue
T21102	18965-18969	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T21101	18862-18867	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T21100	18849-18854	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T21099	18470-18475	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T21098	18375-18380	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T21097	18142-18147	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T21096	17928-17933	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T21095	17879-17884	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T21094	17432-17437	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T21093	17345-17350	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T21092	17269-17274	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T21091	17175-17180	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T21090	16801-16806	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T6013	14380-14388	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T6012	14293-14301	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T6011	14380-14388	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T6010	14293-14301	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T6009	14024-14032	http://purl.obolibrary.org/obo/GO_0016020	denotes	Membrane
T6008	13618-13622	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T5101	12115-12123	http://purl.obolibrary.org/obo/GO_0009274	denotes	envelope
T5100	12575-12580	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T5099	12432-12437	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T5098	12383-12388	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T5097	12314-12319	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T5609	13295-13299	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T5608	13192-13196	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T5607	13165-13169	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T119	527-532	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T6792	15806-15810	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T6791	15728-15732	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T5096	11207-11212	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T4332	9811-9819	http://purl.obolibrary.org/obo/GO_0009274	denotes	envelope
T4095	9296-9301	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T4665	11018-11023	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T3602	8634-8639	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T2924	7503-7508	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T2923	7360-7365	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T2922	7265-7270	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T2921	6858-6863	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T2920	6304-6308	http://purl.obolibrary.org/obo/GO_0005623	denotes	Cell
T2813	6217-6222	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T2812	6181-6186	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T1053	5068-5072	http://purl.obolibrary.org/obo/GO_0019013	denotes	core
T1052	5573-5578	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T1051	4854-4859	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T1050	3603-3608	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T1049	3079-3084	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T1048	3059-3064	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T1047	2978-2982	http://purl.obolibrary.org/obo/GO_0018995	denotes	host

sentences

Id	Subject	Object	Predicate	Lexical cue
T57	0-133	Sentence	denotes	Regulation of Mycobacterium tuberculosis-Dependent HIV-1 Transcription Reveals a New Role for NFAT5 in the Toll-Like Receptor Pathway
T21053	18658-18855	Sentence	denotes	Nucleotide sequences representing the wild-type and NFAT5 binding site-mutated HIV-1Lai LTRs are shown at the bottom of the figure. (C) MTb lysate increases NFAT5 protein levels in monocytic cells.
T21052	18509-18657	Sentence	denotes	Results are from three independent experiments performed in duplicate and adjusted to Renilla luciferase control expression (, p<0.05; *, p<0.01).
T21051	18420-18508	Sentence	denotes	LTR activity was also suppressed in the untreated cells but to a lesser extent (p<0.05).
T21050	18122-18419	Sentence	denotes	After 16 hours, the cells were left untreated or exposed to 10 µg/ml MTb lysate for 8 hours at 37°C. Disruption of NFAT5 binding to the enhancer region significantly suppressed LTR-driven reporter gene expression in comparison to the wild-type LTR when cells were treated with MTb lysate (p<0.01).
T21049	17922-18121	Sentence	denotes	THP-1 cells were transfected with luciferase expression vectors encoding nucleotides 208 to +64 of the wild-type HIV-1Lai LTR and an HIV-1Lai LTR containing the NFAT5 binding site mutations (N5-Mut).
T21048	17635-17921	Sentence	denotes	Results are from three independent experiments performed in duplicate (, p<0.05; *, p<0.01 as compared to unstimulated cultures). (B) Specific disruption of the NFAT5 binding site significantly reduces LTR-reporter gene activity in monocytic cells in response to MTb lysate treatment.
T21047	17463-17634	Sentence	denotes	LTR transcriptional activity for all of the representative LTRs tested was significantly increased in cultures treated with MTb lysate in comparison to untreated cultures.
T21046	17276-17462	Sentence	denotes	Black bars represent individual LTR activities in MTb lysate-treated cells, and dark grey bars represent mean values of LTR activities from each subtype in cells treated with MTb lysate.
T21045	17182-17275	Sentence	denotes	Light grey bars represent mean values of LTR activities from each subtype in untreated cells.
T21044	17098-17181	Sentence	denotes	In the histogram, open bars represent individual LTR activities in untreated cells.
T21043	16976-17097	Sentence	denotes	Cells were then either left untreated or treated with 10 µg/ml MTb lysate for 8 hours before termination of the cultures.
T21042	16795-16975	Sentence	denotes	THP-1 cells (0.8×106/ml) were transfected with each reporter plasmid (0.3 µg/ml) plus the Renilla luciferase control plasmid pRL-TK (0.03 µg/ml) and incubated at 37°C for 16 hours.
T21041	16552-16794	Sentence	denotes	(A) MTb stimulation increases activity of LTRs derived from HIV-1 subtypes B, C, and E. HIV-1 LTRs (−208 to +64 nt relative to the transcription start site) from representative subtype B, C, and E viral isolates were cloned into plasmid pGL3.
T21040	16471-16551	Sentence	denotes	NFAT5 interaction with the LTR is important for MTb-induced HIV-1 transcription.
T6771	16168-16426	Sentence	denotes	Subtype C LTRs displayed the strongest activity, while the LTRs from subtype E isolates consistently showed the weakest activity (Figure 1A), consistent with previous studies demonstrating subtype-specific LTR activity that used TNF as a stimulus [38], [39].
T6770	15984-16167	Sentence	denotes	Upon stimulation, the B, C, and E LTR-driven reporters demonstrated a significant enhancement in luciferase activity (Figure 1A) and the magnitude of this effect was subtype-specific.
T6769	15834-15983	Sentence	denotes	We note that MTb lysate induces inflammatory responses in monocytes that resemble those induced in response to live MTb (see for example, [35]–[37]).
T6768	15686-15833	Sentence	denotes	After transfection of the monocytic THP-1 cell line with these plasmids, cells were stimulated with an irradiated whole cell lysate of MTb (H37Rv).
T6767	15406-15685	Sentence	denotes	To compare the functional impact of MTb stimulation on subtype-specific HIV-1 LTR activity, we first constructed reporter plasmids containing viral subtype B, C, and E LTRs (−208 to + 64 nt relative to the transcription start site) linked to the firefly luciferase reporter gene.
T6766	15343-15405	Sentence	denotes	MTb increases HIV-1 LTR activity of HIV-1 subtypes B, C, and E
T6679	15180-15332	Sentence	denotes	Comparison between two groups was performed using the paired Student t-Test with the aid of Microsoft Excel software. p≤0.05 was considered significant.
T6678	15125-15179	Sentence	denotes	Where applicable, results are expressed as mean ± SEM.
T6677	15104-15124	Sentence	denotes	Statistical analysis
T6532	14999-15102	Sentence	denotes	The results were normalized using β-actin mRNA as an internal control and expressed as relative values.
T6531	14893-14998	Sentence	denotes	The reaction conditions were 95°C for 10 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min.
T6530	14767-14892	Sentence	denotes	The mRNA expression levels were determined by SYBR Green-based real-time PCR (Applied Biosystems, www.appliedbiosystems.com).
T6529	14750-14766	Sentence	denotes	Quantitative PCR
T6000	14631-14748	Sentence	denotes	The blot was again washed 3×5 min in TBST and developed with SuperSignal West Pico Chemiluminescent Reagent (Pierce).
T5999	14466-14630	Sentence	denotes	The blot was washed 3×5 min in TBST and incubated in 1∶6000 donkey anti-goat-HRP (Santa Cruz Biotechnology) or goat anti-rabbit-HRP (BioRad) as appropriate for 1 h.
T5998	14216-14465	Sentence	denotes	Primary incubation was carried out with a1∶200 dilution of rabbit anti-NFAT5 antibody (H-300) (Santa Cruz Biotechnology) and a 1∶500 dilution of goat anti-Lamin-B1 antibody (sc-6217; Santa Cruz Biotechnology) in BSA/TBST for 2 h at room temperature.
T5997	14043-14215	Sentence	denotes	The blot was then blocked for 1 h at 37°C in a solution of 4% BSA (Sigma) and 0.1% Tween-20 (BioRad) in a buffer containing 50 mM Tris and 150 mM NaCl at pH 7.6 (BSA/TBST).
T5996	13960-14042	Sentence	denotes	The gel was transferred to a nitrocellulose Trans-Blot Transfer Membrane (BioRad).
T5995	13830-13959	Sentence	denotes	Extracts were boiled for 5 min in 1× Laemmli sample buffer with 5% v/v 2-mercaptoethanol and proteins were separated by SDS-PAGE.
T5994	13612-13829	Sentence	denotes	Whole cell extracts were collected with lysis buffer containing 150 mM NaCl, 50 mM Tris–HCl, pH 7.5, 1% Triton, 10% glycerol, and 1 tablet of Complete EDTA-free Protease Inhibitor Cocktail (Roche) per 25 ml of buffer.
T5993	13599-13611	Sentence	denotes	Western blot
T5072	12551-12646	Sentence	denotes	Successfully transduced cells were selected and expanded by treatment with 0.8 µg/ml puromycin.
T5071	12202-12550	Sentence	denotes	Supernatants were collected 48 hours post-transfection, clarified by centrifugation, and stored at −80°C. THP-1 cells were transduced with the lentiviral particles by culturing the cells with supernatants from the virus-producing cells in the presence of 8 µg/ml polybrene (Millipore, www.millipore.com) and spinoculation for two hours at 2000 RPM.
T5605	13379-13597	Sentence	denotes	Colony-forming unit (CFU) analysis was performed and on days 4 and 7 the average CFU counts were 6×103 and 5×104, respectively, confirming that mycobacteria levels increased over the course of infection of primary MDM.
T5604	13182-13378	Sentence	denotes	Bacterial cell numbers were determined by measurement of OD650 before further dilution with RPMI 1640 medium for cell infection studies at 10∶1 PBMC∶ bacilli or 1∶1 MDM∶ bacilli and THP-1∶bacilli.
T5603	13023-13181	Sentence	denotes	Bacteria were then plated, washed with PBS, resuspended in PBS, and passed through a 5 µm filter to ensure that the bacteria were in a single cell suspension.
T5602	12914-13022	Sentence	denotes	The cultures were grown to an OD650 of 0.4 at 37°C to ensure that they were in the logarithmic growth phase.
T5601	12660-12913	Sentence	denotes	The MTb clinical strain CDC1551 was prepared by adding 100 µl of frozen bacteria stock into 10 ml of Middlebrook 7H9 medium (Difco BD, www.bd.com) supplemented with albumin dextrose complex (ADC) and 0.05% Tween 80 (Sigma-Aldrich, www.sigmaaldrich.com).
T5600	12648-12659	Sentence	denotes	MTb culture
T4323	10211-10352	Sentence	denotes	HIV-1Lai/Bal-Env and HIV-198IN22 mutant viruses were constructed by introducing point mutations using standard PCR-based mutagenesis methods.
T4322	10061-10210	Sentence	denotes	The infectious molecular clone of HIV-198IN22 was constructed using DNA extracted from PBMC that were infected with a primary isolate of HIV-198IN22.
T4321	9990-10060	Sentence	denotes	The HIV-1Lai/Bal-Env chimeric virus uses CCR5 as a secondary receptor.
T4320	9592-9989	Sentence	denotes	The plasmid encoding the full-length infectious molecular clone of HIV-1Lai was obtained from the NIH AIDS Reagent and Reference Program. The HIV-1Lai/Bal-Env infectious molecular clone was constructed by replacing the envelope (env) gp160 amino acids 103–717 of the HIV-1Lai (B subtype that utilizes CXCR4) molecular clone with the corresponding region of HIV-1Bal (B subtype that utilizes CCR5).
T4319	9558-9591	Sentence	denotes	HIV-1 infectious molecular clones
T4083	9479-9556	Sentence	denotes	Quantitative DNase I footprinting was performed as previously described [31].
T4082	9403-9478	Sentence	denotes	Recombinant p50 and p65 were purchased (Active Motif, www.activemotif.com).
T4081	9191-9402	Sentence	denotes	Recombinant NFAT5 (amino acids 175–471) with an N-terminal 6× His tag was expressed in E. coli BL21(DE3) cells (Stratagene; www.stratagene.com) and purified under native conditions using Ni-NTA agarose (Qiagen).
T4080	9157-9190	Sentence	denotes	Quantitative DNase I footprinting
T4652	11067-11192	Sentence	denotes	MDM were transfected two times for efficient knock down of NFAT5 expression before infection experiments were performed [31].
T4651	10965-11066	Sentence	denotes	The cultures were left at 37°C overnight after which cells were washed and incubated in fresh medium.
T4650	10686-10964	Sentence	denotes	MDM were transfected in 6-well plates using 1 µM of the indicated siRNA in siPORT NeoFX transfection reagent (Ambion Inc., www.ambion.com), prepared as recommended by the manufacturer, in a final volume of 750 µl in Macrophage-SFM medium plus 5% heat-inactivated human AB serum.
T4649	10536-10685	Sentence	denotes	As described, a control for non-specific siRNA effects, we used an siRNA targeting the green fluorescent protein (GFP), 5′- GGCTACGTCCAGGAGCGCACC-3′.
T4648	10488-10535	Sentence	denotes	5′-CAACATGCCTGGAATTCAA-3′ (nt 335 to 353) [31].
T4647	10380-10487	Sentence	denotes	An siRNA was constructed (Ambion Inc., www.ambion.com) to target a sequence unique to the NFAT5 transcript:
T4646	10354-10379	Sentence	denotes	siRNA transfection of MDM
T3591	9012-9155	Sentence	denotes	Reporter gene expression was quantitated by dual-luciferase reporter assay according to the manufacturer's protocol (Promega; www.promega.com).
T3590	8870-9011	Sentence	denotes	Cells were incubated at 37°C for 16 hours after which they were stimulated with 10 µg/ml MTb CDC1551 lysate or left unstimulated for 8 hours.
T3589	8628-8869	Sentence	denotes	THP-1 cells (0.8×106/ml) were transfected with 0.3 µg/ml LTR wild-type (WT) or mutated reporter plasmids in combination with 0.03 µg/ml Renilla luciferase (pRL-TK) control vector using Effectene transfection reagent (Qiagen; www.qiagen.com).
T3588	8503-8627	Sentence	denotes	The HIV-1Lai NFAT5 binding site-mutant (N5-Mut) reporter plasmid was created by standard PCR-based mutagenesis methods [34].
T3587	8425-8502	Sentence	denotes	Sequences were aligned and analyzed with CLUSTAL W (www.ebi.ac.uk/clustalw/).
T3586	8020-8424	Sentence	denotes	LTR reporter plasmids were constructed by inserting nucleotides −208 to +64 relative to the transcriptional initiation site of HIV-1Lai, HIV-1Bal (B subtype), HIV-198IN17, HIV-198IN22, HIV-198CH01, HIV-1CM9 (C subtype), HIV-193TH64, HIV-192TH53, HIV-192TH51, and HIV-1KR25 (E subtype) into the reporter vector pGL3 (Promega BioSciences, www.promega.com) using Xho I and Hind III restriction enzyme sites.
T3585	7976-8019	Sentence	denotes	LTR plasmid construction and reporter assay
T2910	7498-7667	Sentence	denotes	293T cells were obtained from ATCC (www.atcc.org) and were maintained in Dulbecco's Modified Eagle's medium (DMEM) (Gibco, www.invitrogen.com) supplemented with 10% FCS.
T2909	7354-7497	Sentence	denotes	THP-1 cells were obtained from ATCC (www.atcc.org) and cultured in RPMI 1640 medium supplemented with 10% FCS (BioWhittaker, www.lonzabio.com).
T2908	7235-7353	Sentence	denotes	More than 95% of the adherent cells obtained with this technique were CD14+ macrophages as verified by flow cytometry.
T2907	7082-7234	Sentence	denotes	The cell cultures were incubated at 37°C and 5% CO2 for 5 days, after which supernatant was replaced with fresh medium lacking MCSF before manipulation.
T2906	6655-7081	Sentence	denotes	Human monocytes were isolated from PBMC preparations by positive selection with CD14 microbeads from Miltenyi Biotec (www.miltenyibiotec.com) as described by the manufacturer, and were cultured at 1×106 cells per well in 6-well plates in Macrophage-SFM medium (Gibco, www.invitrogen.com) supplemented with 15 ng/ml recombinant human MCSF (R&D, www.rndsystems.com) and 5% heat-inactivated human AB serum (Nabi, Boca Raton, FL).
T2905	6317-6654	Sentence	denotes	PBMC from normal unidentified donors were isolated by Ficoll-Hypaque (Pharmacia Corporation, Peapack, NJ) density gradient centrifugation and were cultured in RPMI 1640 medium with 2 mM L-glutamine (BioWhittaker, Inc., Walkersville, MD) supplemented with 10% heat-inactivated fetal calf serum (FCS) (Gemini Bio-Products, www.gembio.com).
T2904	6304-6316	Sentence	denotes	Cell culture
T3467	7677-7974	Sentence	denotes	HIV-1Bal, HIV-1Lai, HIV-193TH64, HIV-192TH51, HIV-192TH53, HIV-198CH01, and HIV-198IN22 were obtained from The Centralized Facility for AIDS Reagents, National Institute for Biological Standard and Control (NIBSC), United Kingdom. HIV-1KR25 was isolated in our laboratory as described before [33].
T3466	7669-7676	Sentence	denotes	Viruses
T2811	6123-6302	Sentence	denotes	In our studies we used unidentified human discarded blood cells (peripheral blood mononuclear cells, PBMC), which we obtained from the Blood Bank of Children's Hospital in Boston.
T2810	6106-6122	Sentence	denotes	Ethics statement
T941	5873-6081	Sentence	denotes	Thus, these results expand the known stimuli of NFAT5 expression to the PRR-mediated innate immune response, and demonstrate that NFAT5 is a critical modulator of MTb-induced enhancement of HIV-1 replication.
T940	5749-5872	Sentence	denotes	Moreover, we demonstrate that MTb infection increases NFAT5 gene expression in human monocytes in a MyD88-dependent manner.
T939	5587-5748	Sentence	denotes	Here, we show that NFAT5 and its cognate binding site are of crucial importance for efficient MTb-induced stimulation of HIV-1 replication in human MDM and PBMC.
T938	5261-5586	Sentence	denotes	Given that NFAT5 has previously been shown to be transcriptionally activated by the MAP kinase p38, which is downstream of MyD88 signaling, [32], we speculated that NFAT5 may also be involved in MTb-induced activation of HIV-1 replication via a TLR-mediated pathway in monocytes and peripheral blood mononuclear cells (PBMC).
T937	5039-5260	Sentence	denotes	This NFAT5 site overlaps the core NF-κB binding motifs in the LTR and is required for constitutive replication of representative HIV-1 subtype B, C, and E isolates in human primary monocyte-derived macrophages (MDM) [31].
T936	4764-5038	Sentence	denotes	We previously showed that the most primordial member of the nuclear factor of activated T cells (NFAT) family, NFAT5 (also known as TonEBP), binds to a site within the HIV-1 LTR that is highly conserved across all HIV-1 subtypes, and is also conserved in HIV-2 and SIV LTRs.
T935	4567-4763	Sentence	denotes	By contrast, HIV-1 subtypes C and E, which have spread disproportionately in TB-burdened sub-Saharan Africa and southeast Asia, have three and one NF-κB binding sites, respectively [1], [28]–[30].
T63	1402-1511	Sentence	denotes	These findings also demonstrate a general role for NFAT5 in TLR- and MTb-mediated control of gene expression.
T62	1189-1401	Sentence	denotes	Thus, the innate immune response to MTb infection induces NFAT5 gene and protein expression, and NFAT5 plays a crucial role in MTb regulation of HIV-1 replication via a direct interaction with the viral promoter.
T61	973-1188	Sentence	denotes	Furthermore, silencing by RNAi of key components of the TLR pathway in human monocytes, including the downstream signaling molecules MyD88, IRAK1, and TRAF6, significantly inhibits MTb-induced NFAT5 gene expression.
T60	669-972	Sentence	denotes	We show that NFAT5 gene and protein expression are strongly induced by MTb, which is a Toll-like receptor (TLR) ligand, and that an intact NFAT5 binding site in the viral promoter of R5-tropic HIV-1 subtype B and subtype C molecular clones is required for efficent induction of HIV-1 replication by MTb.
T59	415-668	Sentence	denotes	Here, we demonstrate that silencing of the expression of the transcription factor nuclear factor of activated T cells 5 (NFAT5) by RNA interference (RNAi) inhibits Mycobacterium tuberculosis (MTb)-stimulated HIV-1 replication in co-infected macrophages.
T58	194-414	Sentence	denotes	Tuberculosis (TB) disease in HIV co-infected patients contributes to increased mortality by activating innate and adaptive immune signaling cascades that stimulate HIV-1 replication, leading to an increase in viral load.
T5070	11916-12201	Sentence	denotes	Lentiviruses encoding shRNA sequences were generated by transfecting the packaging cell line HEK-293T with the shRNA-encoding pLKO.1 plasmids in combination with the packaging plasmid psPAX2 and the envelope plasmid pMD2.G using Effectene transfection reagent (Qiagen, www.qiagen.com).
T5069	11230-11915	Sentence	denotes	The lentiviral plasmid pLKO.1 expressing shRNA targeting human MyD88 was purchased from Open Biosystems (www.openbiosystems.com) and was validated in our laboratory. shRNA targeting human IRAK1 (forward primer 5′-CCGGAGCAGCTGTCCAGGTTTCGTCTCATAAAACCTGGACAGCTGCTCCTTTTTG-3′, reverse primer 5′-AATTCAAAAAGGAGCAGCTGTCCAGGTTTTATGAGACGAAACCTGGACAGCTGCT-3′ mRNA (IRAK1 mRNA target sequence is underlined) and human TRAF6 (forward primer 5′-CCGGAGAAACCTGTTGTGATTCGTCTCATAAATCACAACAGGTTTCTCCTTTTTG-3′, reverse primer 5′-AATTCAAAAAGGAGAAACCTGTTGTGATTTATGAGACGAATCACAACAGGTTTCT-3′ (TRAF6 mRNA target sequence is underlined) were designed in our laboratory and were cloned into the pLKO.1 plasmid.
T5068	11194-11229	Sentence	denotes	Stable THP-1 cells expressing shRNA
T934	4371-4566	Sentence	denotes	For example, HIV-1 subtype B, the most highly characterized viral subtype and the primary cause of infection in the Americas, Europe, Japan, and Australia, has two tandem NF-κB motifs in its LTR.
T933	4218-4370	Sentence	denotes	Notably, HIV-1 comprises several subtypes, and the LTR of each subtype is unique with respect to the number and organization of activator binding sites.
T932	3893-4217	Sentence	denotes	TRAF6 activates IκB kinase (IKK) and mitogen-activated protein (MAP) kinases that, in turn, ultimately induce activation of specific transcription factor families, including the NF-κB and AP-1 families, which have been shown to associate with the HIV-1 LTR and to drive its transcription ([22], [25]–[27], reviewed in [10]).
T931	3677-3892	Sentence	denotes	Engagement of TLR2 results in engagement of the adaptor protein MyD88 and the subsequent recruitment of several kinases, including IRAK1 and IRAK4, and the ubiquitin ligase TRAF6 ([21]–[23], reviewed in [10], [24]).
T930	3574-3676	Sentence	denotes	The primary PRR on monocytic cells triggered by MTb infection is toll-like receptor (TLR) 2 [16]–[20].
T929	3353-3573	Sentence	denotes	Furthermore, the progressive immune compromise associated with HIV-1 infection itself is a major cause of latent MTb reactivation, as well as increased susceptibility to primary TB infection ([11]–[15], reviewed in [8]).
T928	3158-3352	Sentence	denotes	Activation of HIV-1 replication via these MTb-induced pathways ultimately leads to higher viral loads and, in turn, expedited CD4 T cell loss and progression to AIDS ([7], reviewed in [8]–[10]).
T927	2905-3157	Sentence	denotes	Furthermore, inflammatory cytokines and chemokines produced by the human host in response to MTb infection activate signal transduction pathways in CD4 T cells and monocytic cells that also result in transcriptional activation of the HIV-1 LTR [4]–[6].
T926	2708-2904	Sentence	denotes	Direct engagement of pathogen recognition receptors (PRRs) by MTb on mononuclear phagocytes activates signaling cascades that directly induce transcription from the proviral LTR (reviewed in [3]).
T925	2501-2707	Sentence	denotes	Mycobacterium tuberculosis (MTb), the causative agent of tuberculosis (TB), is the most common co-infection and cause of death in patients infected with human immunodeficiency virus type 1 (HIV-1) [1], [2].
T1	0-133	Sentence	denotes	Regulation of Mycobacterium tuberculosis-Dependent HIV-1 Transcription Reveals a New Role for NFAT5 in the Toll-Like Receptor Pathway
T2	134-183	Sentence	denotes	NFAT5 Modulation of MTb-Induced HIV-1 Replication
T3	185-193	Sentence	denotes	Abstract
T4	194-414	Sentence	denotes	Tuberculosis (TB) disease in HIV co-infected patients contributes to increased mortality by activating innate and adaptive immune signaling cascades that stimulate HIV-1 replication, leading to an increase in viral load.
T5	415-668	Sentence	denotes	Here, we demonstrate that silencing of the expression of the transcription factor nuclear factor of activated T cells 5 (NFAT5) by RNA interference (RNAi) inhibits Mycobacterium tuberculosis (MTb)-stimulated HIV-1 replication in co-infected macrophages.
T6	669-972	Sentence	denotes	We show that NFAT5 gene and protein expression are strongly induced by MTb, which is a Toll-like receptor (TLR) ligand, and that an intact NFAT5 binding site in the viral promoter of R5-tropic HIV-1 subtype B and subtype C molecular clones is required for efficent induction of HIV-1 replication by MTb.
T7	973-1188	Sentence	denotes	Furthermore, silencing by RNAi of key components of the TLR pathway in human monocytes, including the downstream signaling molecules MyD88, IRAK1, and TRAF6, significantly inhibits MTb-induced NFAT5 gene expression.
T8	1189-1401	Sentence	denotes	Thus, the innate immune response to MTb infection induces NFAT5 gene and protein expression, and NFAT5 plays a crucial role in MTb regulation of HIV-1 replication via a direct interaction with the viral promoter.
T9	1402-1511	Sentence	denotes	These findings also demonstrate a general role for NFAT5 in TLR- and MTb-mediated control of gene expression.
T10	1513-1527	Sentence	denotes	Author Summary
T11	1528-1662	Sentence	denotes	The major cause of AIDS deaths globally has been tuberculosis (TB), which is caused by the bacterium Mycobacterium tuberculosis (MTb).
T12	1663-1844	Sentence	denotes	Co-infection with MTb exacerbates human immunodeficiency virus type1 (HIV-1) replication and disease progression via both innate and adaptive host immune responses to MTb infection.
T13	1845-2017	Sentence	denotes	In this report, we present evidence that the transcription factor NFAT5 plays a crucial role in MTb-induced HIV-1 replication in human peripheral blood cells and monocytes.
T14	2018-2238	Sentence	denotes	We also show that MTb infection itself stimulates NFAT5 gene expression in human monocytes and that its expression involves the TLR signalling pathway and requires the downstream adaptor proteins MyD88, IRAK1, and TRAF6.
T15	2239-2486	Sentence	denotes	This identification of a novel role for NFAT5 in TB/HIV-1 co-infection reveals that NFAT5 is a major mediator of TLR-dependent gene expression and thus provides a potential new therapeutic target for treatment of HIV-1 and possibly other diseases.
T16	2488-2500	Sentence	denotes	Introduction
T17	2501-2707	Sentence	denotes	Mycobacterium tuberculosis (MTb), the causative agent of tuberculosis (TB), is the most common co-infection and cause of death in patients infected with human immunodeficiency virus type 1 (HIV-1) [1], [2].
T18	2708-2904	Sentence	denotes	Direct engagement of pathogen recognition receptors (PRRs) by MTb on mononuclear phagocytes activates signaling cascades that directly induce transcription from the proviral LTR (reviewed in [3]).
T19	2905-3157	Sentence	denotes	Furthermore, inflammatory cytokines and chemokines produced by the human host in response to MTb infection activate signal transduction pathways in CD4 T cells and monocytic cells that also result in transcriptional activation of the HIV-1 LTR [4]–[6].
T20	3158-3352	Sentence	denotes	Activation of HIV-1 replication via these MTb-induced pathways ultimately leads to higher viral loads and, in turn, expedited CD4 T cell loss and progression to AIDS ([7], reviewed in [8]–[10]).
T21	3353-3573	Sentence	denotes	Furthermore, the progressive immune compromise associated with HIV-1 infection itself is a major cause of latent MTb reactivation, as well as increased susceptibility to primary TB infection ([11]–[15], reviewed in [8]).
T22	3574-3676	Sentence	denotes	The primary PRR on monocytic cells triggered by MTb infection is toll-like receptor (TLR) 2 [16]–[20].
T23	3677-3892	Sentence	denotes	Engagement of TLR2 results in engagement of the adaptor protein MyD88 and the subsequent recruitment of several kinases, including IRAK1 and IRAK4, and the ubiquitin ligase TRAF6 ([21]–[23], reviewed in [10], [24]).
T24	3893-4217	Sentence	denotes	TRAF6 activates IκB kinase (IKK) and mitogen-activated protein (MAP) kinases that, in turn, ultimately induce activation of specific transcription factor families, including the NF-κB and AP-1 families, which have been shown to associate with the HIV-1 LTR and to drive its transcription ([22], [25]–[27], reviewed in [10]).
T25	4218-4370	Sentence	denotes	Notably, HIV-1 comprises several subtypes, and the LTR of each subtype is unique with respect to the number and organization of activator binding sites.
T26	4371-4566	Sentence	denotes	For example, HIV-1 subtype B, the most highly characterized viral subtype and the primary cause of infection in the Americas, Europe, Japan, and Australia, has two tandem NF-κB motifs in its LTR.
T27	4567-4763	Sentence	denotes	By contrast, HIV-1 subtypes C and E, which have spread disproportionately in TB-burdened sub-Saharan Africa and southeast Asia, have three and one NF-κB binding sites, respectively [1], [28]–[30].
T28	4764-5038	Sentence	denotes	We previously showed that the most primordial member of the nuclear factor of activated T cells (NFAT) family, NFAT5 (also known as TonEBP), binds to a site within the HIV-1 LTR that is highly conserved across all HIV-1 subtypes, and is also conserved in HIV-2 and SIV LTRs.
T29	5039-5260	Sentence	denotes	This NFAT5 site overlaps the core NF-κB binding motifs in the LTR and is required for constitutive replication of representative HIV-1 subtype B, C, and E isolates in human primary monocyte-derived macrophages (MDM) [31].
T30	5261-5586	Sentence	denotes	Given that NFAT5 has previously been shown to be transcriptionally activated by the MAP kinase p38, which is downstream of MyD88 signaling, [32], we speculated that NFAT5 may also be involved in MTb-induced activation of HIV-1 replication via a TLR-mediated pathway in monocytes and peripheral blood mononuclear cells (PBMC).
T31	5587-5748	Sentence	denotes	Here, we show that NFAT5 and its cognate binding site are of crucial importance for efficient MTb-induced stimulation of HIV-1 replication in human MDM and PBMC.
T32	5749-5872	Sentence	denotes	Moreover, we demonstrate that MTb infection increases NFAT5 gene expression in human monocytes in a MyD88-dependent manner.
T33	5873-6081	Sentence	denotes	Thus, these results expand the known stimuli of NFAT5 expression to the PRR-mediated innate immune response, and demonstrate that NFAT5 is a critical modulator of MTb-induced enhancement of HIV-1 replication.
T34	6083-6104	Sentence	denotes	Materials and Methods
T35	6106-6122	Sentence	denotes	Ethics statement
T36	6123-6302	Sentence	denotes	In our studies we used unidentified human discarded blood cells (peripheral blood mononuclear cells, PBMC), which we obtained from the Blood Bank of Children's Hospital in Boston.
T37	6304-6316	Sentence	denotes	Cell culture
T38	6317-6654	Sentence	denotes	PBMC from normal unidentified donors were isolated by Ficoll-Hypaque (Pharmacia Corporation, Peapack, NJ) density gradient centrifugation and were cultured in RPMI 1640 medium with 2 mM L-glutamine (BioWhittaker, Inc., Walkersville, MD) supplemented with 10% heat-inactivated fetal calf serum (FCS) (Gemini Bio-Products, www.gembio.com).
T39	6655-7081	Sentence	denotes	Human monocytes were isolated from PBMC preparations by positive selection with CD14 microbeads from Miltenyi Biotec (www.miltenyibiotec.com) as described by the manufacturer, and were cultured at 1×106 cells per well in 6-well plates in Macrophage-SFM medium (Gibco, www.invitrogen.com) supplemented with 15 ng/ml recombinant human MCSF (R&D, www.rndsystems.com) and 5% heat-inactivated human AB serum (Nabi, Boca Raton, FL).
T40	7082-7234	Sentence	denotes	The cell cultures were incubated at 37°C and 5% CO2 for 5 days, after which supernatant was replaced with fresh medium lacking MCSF before manipulation.
T41	7235-7353	Sentence	denotes	More than 95% of the adherent cells obtained with this technique were CD14+ macrophages as verified by flow cytometry.
T42	7354-7497	Sentence	denotes	THP-1 cells were obtained from ATCC (www.atcc.org) and cultured in RPMI 1640 medium supplemented with 10% FCS (BioWhittaker, www.lonzabio.com).
T43	7498-7667	Sentence	denotes	293T cells were obtained from ATCC (www.atcc.org) and were maintained in Dulbecco's Modified Eagle's medium (DMEM) (Gibco, www.invitrogen.com) supplemented with 10% FCS.
T44	7669-7676	Sentence	denotes	Viruses
T45	7677-7907	Sentence	denotes	HIV-1Bal, HIV-1Lai, HIV-193TH64, HIV-192TH51, HIV-192TH53, HIV-198CH01, and HIV-198IN22 were obtained from The Centralized Facility for AIDS Reagents, National Institute for Biological Standard and Control (NIBSC), United Kingdom.
T46	7908-7974	Sentence	denotes	HIV-1KR25 was isolated in our laboratory as described before [33].
T47	7976-8019	Sentence	denotes	LTR plasmid construction and reporter assay
T48	8020-8424	Sentence	denotes	LTR reporter plasmids were constructed by inserting nucleotides −208 to +64 relative to the transcriptional initiation site of HIV-1Lai, HIV-1Bal (B subtype), HIV-198IN17, HIV-198IN22, HIV-198CH01, HIV-1CM9 (C subtype), HIV-193TH64, HIV-192TH53, HIV-192TH51, and HIV-1KR25 (E subtype) into the reporter vector pGL3 (Promega BioSciences, www.promega.com) using Xho I and Hind III restriction enzyme sites.
T49	8425-8502	Sentence	denotes	Sequences were aligned and analyzed with CLUSTAL W (www.ebi.ac.uk/clustalw/).
T50	8503-8627	Sentence	denotes	The HIV-1Lai NFAT5 binding site-mutant (N5-Mut) reporter plasmid was created by standard PCR-based mutagenesis methods [34].
T51	8628-8869	Sentence	denotes	THP-1 cells (0.8×106/ml) were transfected with 0.3 µg/ml LTR wild-type (WT) or mutated reporter plasmids in combination with 0.03 µg/ml Renilla luciferase (pRL-TK) control vector using Effectene transfection reagent (Qiagen; www.qiagen.com).
T52	8870-9011	Sentence	denotes	Cells were incubated at 37°C for 16 hours after which they were stimulated with 10 µg/ml MTb CDC1551 lysate or left unstimulated for 8 hours.
T53	9012-9155	Sentence	denotes	Reporter gene expression was quantitated by dual-luciferase reporter assay according to the manufacturer's protocol (Promega; www.promega.com).
T54	9157-9190	Sentence	denotes	Quantitative DNase I footprinting
T55	9191-9402	Sentence	denotes	Recombinant NFAT5 (amino acids 175–471) with an N-terminal 6× His tag was expressed in E. coli BL21(DE3) cells (Stratagene; www.stratagene.com) and purified under native conditions using Ni-NTA agarose (Qiagen).
T56	9403-9478	Sentence	denotes	Recombinant p50 and p65 were purchased (Active Motif, www.activemotif.com).
T57	9479-9556	Sentence	denotes	Quantitative DNase I footprinting was performed as previously described [31].
T58	9558-9591	Sentence	denotes	HIV-1 infectious molecular clones
T59	9592-9729	Sentence	denotes	The plasmid encoding the full-length infectious molecular clone of HIV-1Lai was obtained from the NIH AIDS Reagent and Reference Program.
T60	9730-9989	Sentence	denotes	The HIV-1Lai/Bal-Env infectious molecular clone was constructed by replacing the envelope (env) gp160 amino acids 103–717 of the HIV-1Lai (B subtype that utilizes CXCR4) molecular clone with the corresponding region of HIV-1Bal (B subtype that utilizes CCR5).
T61	9990-10060	Sentence	denotes	The HIV-1Lai/Bal-Env chimeric virus uses CCR5 as a secondary receptor.
T62	10061-10210	Sentence	denotes	The infectious molecular clone of HIV-198IN22 was constructed using DNA extracted from PBMC that were infected with a primary isolate of HIV-198IN22.
T63	10211-10352	Sentence	denotes	HIV-1Lai/Bal-Env and HIV-198IN22 mutant viruses were constructed by introducing point mutations using standard PCR-based mutagenesis methods.
T64	10354-10379	Sentence	denotes	siRNA transfection of MDM
T65	10380-10487	Sentence	denotes	An siRNA was constructed (Ambion Inc., www.ambion.com) to target a sequence unique to the NFAT5 transcript:
T66	10488-10535	Sentence	denotes	5′-CAACATGCCTGGAATTCAA-3′ (nt 335 to 353) [31].
T67	10536-10685	Sentence	denotes	As described, a control for non-specific siRNA effects, we used an siRNA targeting the green fluorescent protein (GFP), 5′- GGCTACGTCCAGGAGCGCACC-3′.
T68	10686-10964	Sentence	denotes	MDM were transfected in 6-well plates using 1 µM of the indicated siRNA in siPORT NeoFX transfection reagent (Ambion Inc., www.ambion.com), prepared as recommended by the manufacturer, in a final volume of 750 µl in Macrophage-SFM medium plus 5% heat-inactivated human AB serum.
T69	10965-11066	Sentence	denotes	The cultures were left at 37°C overnight after which cells were washed and incubated in fresh medium.
T70	11067-11192	Sentence	denotes	MDM were transfected two times for efficient knock down of NFAT5 expression before infection experiments were performed [31].
T71	11194-11229	Sentence	denotes	Stable THP-1 cells expressing shRNA
T72	11230-11915	Sentence	denotes	The lentiviral plasmid pLKO.1 expressing shRNA targeting human MyD88 was purchased from Open Biosystems (www.openbiosystems.com) and was validated in our laboratory. shRNA targeting human IRAK1 (forward primer 5′-CCGGAGCAGCTGTCCAGGTTTCGTCTCATAAAACCTGGACAGCTGCTCCTTTTTG-3′, reverse primer 5′-AATTCAAAAAGGAGCAGCTGTCCAGGTTTTATGAGACGAAACCTGGACAGCTGCT-3′ mRNA (IRAK1 mRNA target sequence is underlined) and human TRAF6 (forward primer 5′-CCGGAGAAACCTGTTGTGATTCGTCTCATAAATCACAACAGGTTTCTCCTTTTTG-3′, reverse primer 5′-AATTCAAAAAGGAGAAACCTGTTGTGATTTATGAGACGAATCACAACAGGTTTCT-3′ (TRAF6 mRNA target sequence is underlined) were designed in our laboratory and were cloned into the pLKO.1 plasmid.
T73	11916-12201	Sentence	denotes	Lentiviruses encoding shRNA sequences were generated by transfecting the packaging cell line HEK-293T with the shRNA-encoding pLKO.1 plasmids in combination with the packaging plasmid psPAX2 and the envelope plasmid pMD2.G using Effectene transfection reagent (Qiagen, www.qiagen.com).
T74	12202-12307	Sentence	denotes	Supernatants were collected 48 hours post-transfection, clarified by centrifugation, and stored at −80°C.
T75	12308-12550	Sentence	denotes	THP-1 cells were transduced with the lentiviral particles by culturing the cells with supernatants from the virus-producing cells in the presence of 8 µg/ml polybrene (Millipore, www.millipore.com) and spinoculation for two hours at 2000 RPM.
T76	12551-12646	Sentence	denotes	Successfully transduced cells were selected and expanded by treatment with 0.8 µg/ml puromycin.
T77	12648-12659	Sentence	denotes	MTb culture
T78	12660-12913	Sentence	denotes	The MTb clinical strain CDC1551 was prepared by adding 100 µl of frozen bacteria stock into 10 ml of Middlebrook 7H9 medium (Difco BD, www.bd.com) supplemented with albumin dextrose complex (ADC) and 0.05% Tween 80 (Sigma-Aldrich, www.sigmaaldrich.com).
T79	12914-13022	Sentence	denotes	The cultures were grown to an OD650 of 0.4 at 37°C to ensure that they were in the logarithmic growth phase.
T80	13023-13181	Sentence	denotes	Bacteria were then plated, washed with PBS, resuspended in PBS, and passed through a 5 µm filter to ensure that the bacteria were in a single cell suspension.
T81	13182-13378	Sentence	denotes	Bacterial cell numbers were determined by measurement of OD650 before further dilution with RPMI 1640 medium for cell infection studies at 10∶1 PBMC∶ bacilli or 1∶1 MDM∶ bacilli and THP-1∶bacilli.
T82	13379-13597	Sentence	denotes	Colony-forming unit (CFU) analysis was performed and on days 4 and 7 the average CFU counts were 6×103 and 5×104, respectively, confirming that mycobacteria levels increased over the course of infection of primary MDM.
T83	13599-13611	Sentence	denotes	Western blot
T84	13612-13829	Sentence	denotes	Whole cell extracts were collected with lysis buffer containing 150 mM NaCl, 50 mM Tris–HCl, pH 7.5, 1% Triton, 10% glycerol, and 1 tablet of Complete EDTA-free Protease Inhibitor Cocktail (Roche) per 25 ml of buffer.
T85	13830-13959	Sentence	denotes	Extracts were boiled for 5 min in 1× Laemmli sample buffer with 5% v/v 2-mercaptoethanol and proteins were separated by SDS-PAGE.
T86	13960-14042	Sentence	denotes	The gel was transferred to a nitrocellulose Trans-Blot Transfer Membrane (BioRad).
T87	14043-14215	Sentence	denotes	The blot was then blocked for 1 h at 37°C in a solution of 4% BSA (Sigma) and 0.1% Tween-20 (BioRad) in a buffer containing 50 mM Tris and 150 mM NaCl at pH 7.6 (BSA/TBST).
T88	14216-14465	Sentence	denotes	Primary incubation was carried out with a1∶200 dilution of rabbit anti-NFAT5 antibody (H-300) (Santa Cruz Biotechnology) and a 1∶500 dilution of goat anti-Lamin-B1 antibody (sc-6217; Santa Cruz Biotechnology) in BSA/TBST for 2 h at room temperature.
T89	14466-14630	Sentence	denotes	The blot was washed 3×5 min in TBST and incubated in 1∶6000 donkey anti-goat-HRP (Santa Cruz Biotechnology) or goat anti-rabbit-HRP (BioRad) as appropriate for 1 h.
T90	14631-14748	Sentence	denotes	The blot was again washed 3×5 min in TBST and developed with SuperSignal West Pico Chemiluminescent Reagent (Pierce).
T91	14750-14766	Sentence	denotes	Quantitative PCR
T92	14767-14892	Sentence	denotes	The mRNA expression levels were determined by SYBR Green-based real-time PCR (Applied Biosystems, www.appliedbiosystems.com).
T93	14893-14998	Sentence	denotes	The reaction conditions were 95°C for 10 min followed by 40 cycles of 95°C for 15 sec and 60°C for 1 min.
T94	14999-15102	Sentence	denotes	The results were normalized using β-actin mRNA as an internal control and expressed as relative values.
T95	15104-15124	Sentence	denotes	Statistical analysis
T96	15125-15179	Sentence	denotes	Where applicable, results are expressed as mean ± SEM.
T97	15180-15332	Sentence	denotes	Comparison between two groups was performed using the paired Student t-Test with the aid of Microsoft Excel software. p≤0.05 was considered significant.
T98	15334-15341	Sentence	denotes	Results
T99	15343-15405	Sentence	denotes	MTb increases HIV-1 LTR activity of HIV-1 subtypes B, C, and E
T100	15406-15685	Sentence	denotes	To compare the functional impact of MTb stimulation on subtype-specific HIV-1 LTR activity, we first constructed reporter plasmids containing viral subtype B, C, and E LTRs (−208 to + 64 nt relative to the transcription start site) linked to the firefly luciferase reporter gene.
T101	15686-15833	Sentence	denotes	After transfection of the monocytic THP-1 cell line with these plasmids, cells were stimulated with an irradiated whole cell lysate of MTb (H37Rv).
T102	15834-15983	Sentence	denotes	We note that MTb lysate induces inflammatory responses in monocytes that resemble those induced in response to live MTb (see for example, [35]–[37]).
T103	15984-16167	Sentence	denotes	Upon stimulation, the B, C, and E LTR-driven reporters demonstrated a significant enhancement in luciferase activity (Figure 1A) and the magnitude of this effect was subtype-specific.
T104	16168-16426	Sentence	denotes	Subtype C LTRs displayed the strongest activity, while the LTRs from subtype E isolates consistently showed the weakest activity (Figure 1A), consistent with previous studies demonstrating subtype-specific LTR activity that used TNF as a stimulus [38], [39].
T105	16427-16551	Sentence	denotes	10.1371/journal.ppat.1002620.g001 Figure 1 NFAT5 interaction with the LTR is important for MTb-induced HIV-1 transcription.
T106	16552-16639	Sentence	denotes	(A) MTb stimulation increases activity of LTRs derived from HIV-1 subtypes B, C, and E.
T107	16640-16794	Sentence	denotes	HIV-1 LTRs (−208 to +64 nt relative to the transcription start site) from representative subtype B, C, and E viral isolates were cloned into plasmid pGL3.
T108	16795-16975	Sentence	denotes	THP-1 cells (0.8×106/ml) were transfected with each reporter plasmid (0.3 µg/ml) plus the Renilla luciferase control plasmid pRL-TK (0.03 µg/ml) and incubated at 37°C for 16 hours.
T109	16976-17097	Sentence	denotes	Cells were then either left untreated or treated with 10 µg/ml MTb lysate for 8 hours before termination of the cultures.
T110	17098-17181	Sentence	denotes	In the histogram, open bars represent individual LTR activities in untreated cells.
T111	17182-17275	Sentence	denotes	Light grey bars represent mean values of LTR activities from each subtype in untreated cells.
T112	17276-17462	Sentence	denotes	Black bars represent individual LTR activities in MTb lysate-treated cells, and dark grey bars represent mean values of LTR activities from each subtype in cells treated with MTb lysate.
T113	17463-17634	Sentence	denotes	LTR transcriptional activity for all of the representative LTRs tested was significantly increased in cultures treated with MTb lysate in comparison to untreated cultures.
T114	17635-17921	Sentence	denotes	Results are from three independent experiments performed in duplicate (, p<0.05; *, p<0.01 as compared to unstimulated cultures). (B) Specific disruption of the NFAT5 binding site significantly reduces LTR-reporter gene activity in monocytic cells in response to MTb lysate treatment.
T115	17922-18121	Sentence	denotes	THP-1 cells were transfected with luciferase expression vectors encoding nucleotides 208 to +64 of the wild-type HIV-1Lai LTR and an HIV-1Lai LTR containing the NFAT5 binding site mutations (N5-Mut).
T116	18122-18222	Sentence	denotes	After 16 hours, the cells were left untreated or exposed to 10 µg/ml MTb lysate for 8 hours at 37°C.
T117	18223-18419	Sentence	denotes	Disruption of NFAT5 binding to the enhancer region significantly suppressed LTR-driven reporter gene expression in comparison to the wild-type LTR when cells were treated with MTb lysate (p<0.01).
T118	18420-18508	Sentence	denotes	LTR activity was also suppressed in the untreated cells but to a lesser extent (p<0.05).
T119	18509-18657	Sentence	denotes	Results are from three independent experiments performed in duplicate and adjusted to Renilla luciferase control expression (, p<0.05; *, p<0.01).
T120	18658-18855	Sentence	denotes	Nucleotide sequences representing the wild-type and NFAT5 binding site-mutated HIV-1Lai LTRs are shown at the bottom of the figure. (C) MTb lysate increases NFAT5 protein levels in monocytic cells.
T121	18856-18958	Sentence	denotes	THP-1 cells were left untreated (control) or exposed to 10 µg/ml MTb lysate for 8 or 24 hours at 37°C.

ICD10

Id	Subject	Object	Predicate	Lexical cue
T1035	2515-2527	http://purl.bioontology.org/ontology/ICD10/A15-A19.9	denotes	tuberculosis
T116	593-605	http://purl.bioontology.org/ontology/ICD10/A15-A19.9	denotes	tuberculosis
T115	28-40	http://purl.bioontology.org/ontology/ICD10/A15-A19.9	denotes	tuberculosis
T1037	2660-2676	http://purl.bioontology.org/ontology/ICD10/D84.9	denotes	immunodeficiency
T1036	2558-2570	http://purl.bioontology.org/ontology/ICD10/A15-A19.9	denotes	tuberculosis

simple1

Id	Subject	Object	Predicate	Lexical cue
T21120	18815-18820	Protein	denotes	NFAT5
T21119	18603-18613	Protein	denotes	luciferase
T21118	18237-18242	Protein	denotes	NFAT5
T21117	17956-17966	Protein	denotes	luciferase
T21116	16893-16903	Protein	denotes	luciferase
T21115	16471-16476	Protein	denotes	NFAT5
T6535	15033-15040	Protein	denotes	β-actin
T6015	14428-14431	Protein	denotes	BSA
T6014	14105-14108	Protein	denotes	BSA
T4335	9983-9987	Protein	denotes	CCR5
T4334	9893-9898	Protein	denotes	CXCR4
T4333	9826-9831	Protein	denotes	gp160
T4105	9492-9499	Protein	denotes	DNase I
T4104	9423-9426	Protein	denotes	p65
T4103	9415-9418	Protein	denotes	p50
T4102	9203-9208	Protein	denotes	NFAT5
T4101	9170-9177	Protein	denotes	DNase I
T2928	7305-7309	Protein	denotes	CD14
T2927	7209-7213	Protein	denotes	MCSF
T2926	6988-6992	Protein	denotes	MCSF
T2925	6735-6739	Protein	denotes	CD14
T1091	6003-6008	Protein	denotes	NFAT5
T1090	5921-5926	Protein	denotes	NFAT5
T1089	5849-5854	Protein	denotes	MyD88
T1088	5803-5808	Protein	denotes	NFAT5
T1087	5606-5611	Protein	denotes	NFAT5
T168	94-99	Protein	denotes	NFAT5
T6795	16397-16400	Protein	denotes	TNF
T6794	16081-16091	Protein	denotes	luciferase
T6793	15660-15670	Protein	denotes	luciferase
T5108	11801-11806	Protein	denotes	TRAF6
T5107	11638-11643	Protein	denotes	TRAF6
T5106	11586-11591	Protein	denotes	IRAK1
T5105	11418-11423	Protein	denotes	IRAK1
T5104	11293-11298	Protein	denotes	MyD88
T5103	11271-11276	Protein	denotes	shRNA
T5102	11224-11229	Protein	denotes	shRNA
T4669	11126-11131	Protein	denotes	NFAT5
T4668	10650-10653	Protein	denotes	GFP
T4667	10623-10648	Protein	denotes	green fluorescent protein
T4666	10470-10475	Protein	denotes	NFAT5
T3605	9061-9071	Protein	denotes	luciferase
T3604	9012-9025	Protein	denotes	Reporter gene
T3603	8772-8782	Protein	denotes	luciferase
T169	497-534	Protein	denotes	nuclear factor of activated T cells 5
T170	536-541	Protein	denotes	NFAT5
T171	682-687	Protein	denotes	NFAT5
T172	1106-1111	Protein	denotes	MyD88
T173	1113-1118	Protein	denotes	IRAK1
T174	1124-1129	Protein	denotes	TRAF6
T175	1166-1171	Protein	denotes	NFAT5
T176	1247-1252	Protein	denotes	NFAT5
T177	1286-1291	Protein	denotes	NFAT5
T178	1453-1458	Protein	denotes	NFAT5
T1086	5426-5431	Protein	denotes	NFAT5
T1085	5384-5389	Protein	denotes	MyD88
T1084	5272-5277	Protein	denotes	NFAT5
T1083	4896-4902	Protein	denotes	TonEBP
T1082	4875-4880	Protein	denotes	NFAT5
T1081	3893-3898	Protein	denotes	TRAF6
T1080	3850-3855	Protein	denotes	TRAF6
T1079	3818-3823	Protein	denotes	IRAK4
T1078	3808-3813	Protein	denotes	IRAK1
T1077	3741-3746	Protein	denotes	MyD88
T1076	3691-3695	Protein	denotes	TLR2
T1075	3639-3665	Protein	denotes	toll-like receptor (TLR) 2
T1074	3284-3287	Protein	denotes	CD4
T1073	3053-3056	Protein	denotes	CD4

BioNLP16_DUT

Id	Subject	Object	Predicate	Lexical cue
T22253	18805-18814	Positive_regulation	denotes	increases
T22252	18622-18632	Gene_expression	denotes	expression
T22251	18223-18233	Negative_regulation	denotes	Disruption
T22250	18243-18250	Binding	denotes	binding
T22249	17967-17977	Gene_expression	denotes	expression
T22248	16477-16488	Binding	denotes	interaction
T22245	18815-18820	Protein	denotes	NFAT5
T22244	18603-18613	Protein	denotes	luciferase
T22243	18237-18242	Protein	denotes	NFAT5
T22242	17956-17966	Protein	denotes	luciferase
T22241	16893-16903	Protein	denotes	luciferase
T22240	16471-16476	Protein	denotes	NFAT5
T6667	15033-15040	Protein	denotes	β-actin
T7238	16066-16077	Positive_regulation	denotes	enhancement
T7237	16397-16400	Protein	denotes	TNF
T7236	16081-16091	Protein	denotes	luciferase
T7235	15660-15670	Protein	denotes	luciferase
T6489	14428-14431	Protein	denotes	BSA
T6488	14105-14108	Protein	denotes	BSA
T5021	11132-11142	Gene_expression	denotes	expression
T5020	10609-10618	Regulation	denotes	targeting
T5019	11126-11131	Protein	denotes	NFAT5
T5018	10650-10653	Protein	denotes	GFP
T5017	10623-10648	Protein	denotes	green fluorescent protein
T5016	10470-10475	Protein	denotes	NFAT5
T5566	11260-11270	Gene_expression	denotes	expressing
T5565	11213-11223	Gene_expression	denotes	expressing
T5564	11801-11806	Protein	denotes	TRAF6
T5563	11638-11643	Protein	denotes	TRAF6
T5562	11586-11591	Protein	denotes	IRAK1
T5561	11418-11423	Protein	denotes	IRAK1
T5560	11293-11298	Protein	denotes	MyD88
T5559	11271-11276	Protein	denotes	shRNA
T5558	11224-11229	Protein	denotes	shRNA
T4044	9026-9036	Gene_expression	denotes	expression
T4043	9061-9071	Protein	denotes	luciferase
T4042	9012-9025	Protein	denotes	Reporter gene
T4041	8772-8782	Protein	denotes	luciferase
T4614	9983-9987	Protein	denotes	CCR5
T4613	9893-9898	Protein	denotes	CXCR4
T4612	9826-9831	Protein	denotes	gp160
T4290	9265-9274	Gene_expression	denotes	expressed
T4289	9492-9499	Protein	denotes	DNase I
T4288	9423-9426	Protein	denotes	p65
T4287	9415-9418	Protein	denotes	p50
T4286	9203-9208	Protein	denotes	NFAT5
T4285	9170-9177	Protein	denotes	DNase I
T3440	7201-7208	Negative_regulation	denotes	lacking
T3439	7305-7309	Protein	denotes	CD14
T3438	7209-7213	Protein	denotes	MCSF
T3437	6988-6992	Protein	denotes	MCSF
T3436	6735-6739	Protein	denotes	CD14
T2693	5927-5937	Gene_expression	denotes	expression
T2692	5793-5802	Positive_regulation	denotes	increases
T2691	5814-5824	Gene_expression	denotes	expression
T2690	5328-5337	Positive_regulation	denotes	activated
T2689	4905-4910	Binding	denotes	binds
T2688	3707-3717	Binding	denotes	engagement
T2687	3677-3687	Binding	denotes	Engagement
T2686	3766-3777	Binding	denotes	recruitment
T2685	6003-6008	Protein	denotes	NFAT5
T2684	5921-5926	Protein	denotes	NFAT5
T2683	5849-5854	Protein	denotes	MyD88
T2682	5803-5808	Protein	denotes	NFAT5
T2681	5606-5611	Protein	denotes	NFAT5
T2680	5426-5431	Protein	denotes	NFAT5
T2679	5384-5389	Protein	denotes	MyD88
T755	94-99	Protein	denotes	NFAT5
T766	85-89	Regulation	denotes	Role
T2678	5272-5277	Protein	denotes	NFAT5
T2677	4896-4902	Protein	denotes	TonEBP
T2676	4875-4880	Protein	denotes	NFAT5
T2675	3893-3898	Protein	denotes	TRAF6
T2674	3850-3855	Protein	denotes	TRAF6
T2673	3818-3823	Protein	denotes	IRAK4
T2672	3808-3813	Protein	denotes	IRAK1
T2671	3741-3746	Protein	denotes	MyD88
T2670	3691-3695	Protein	denotes	TLR2
T2669	3639-3665	Protein	denotes	toll-like receptor (TLR) 2
T2668	3284-3287	Protein	denotes	CD4
T2667	3053-3056	Protein	denotes	CD4
T756	497-534	Protein	denotes	nuclear factor of activated T cells 5
T757	536-541	Protein	denotes	NFAT5
T758	682-687	Protein	denotes	NFAT5
T759	1106-1111	Protein	denotes	MyD88
T760	1113-1118	Protein	denotes	IRAK1
T761	1124-1129	Protein	denotes	TRAF6
T762	1166-1171	Protein	denotes	NFAT5
T763	1286-1291	Protein	denotes	NFAT5
T764	1247-1252	Protein	denotes	NFAT5
T765	1453-1458	Protein	denotes	NFAT5
T767	441-450	Negative_regulation	denotes	silencing
T768	458-468	Gene_expression	denotes	expression
T769	729-736	Positive_regulation	denotes	induced
T770	705-715	Gene_expression	denotes	expression
T771	986-995	Negative_regulation	denotes	silencing
T772	1154-1165	Positive_regulation	denotes	MTb-induced
T773	1145-1153	Negative_regulation	denotes	inhibits
T774	1177-1187	Gene_expression	denotes	expression
T775	1239-1246	Positive_regulation	denotes	induces
T776	1270-1280	Gene_expression	denotes	expression
T777	1365-1376	Binding	denotes	interaction
R2052	T2671	T2688	themeOf	MyD88,engagement
R2062	T2672	T2688	themeOf	IRAK1,engagement
R2720	T3438	T3440	themeOf	MCSF,lacking
R3236	T4042	T4044	themeOf	Reporter gene,expression
R3389	T4286	T4290	themeOf	NFAT5,expressed
R3968	T5017	T5020	themeOf	green fluorescent protein,targeting
R3969	T5018	T5020	themeOf	GFP,targeting
R3970	T5019	T5021	themeOf	NFAT5,expression
R4405	T5558	T5565	themeOf	shRNA,expressing
R4406	T5559	T5566	themeOf	shRNA,expressing
R4407	T5560	T5566	themeOf	MyD88,expressing
R5839	T7236	T7238	themeOf	luciferase,enhancement
R16537	T22240	T22248	themeOf	NFAT5,interaction
R16538	T22242	T22249	themeOf	luciferase,expression
R16539	T22243	T22251	themeOf	NFAT5,Disruption
R16540	T22243	T22250	themeOf	NFAT5,binding
R16541	T22244	T22252	themeOf	luciferase,expression
R16542	T22245	T22253	themeOf	NFAT5,increases
R16543	T22250	T22251	themeOf	binding,Disruption
R581	T755	T766	themeOf	NFAT5,Role
R582	T757	T768	themeOf	NFAT5,expression
R583	T758	T769	themeOf	NFAT5,induced
R584	T758	T770	themeOf	NFAT5,expression
R585	T759	T771	themeOf	MyD88,silencing
R586	T760	T771	themeOf	IRAK1,silencing
R587	T762	T774	themeOf	NFAT5,expression
R588	T763	T777	themeOf	NFAT5,interaction
R589	T764	T775	themeOf	NFAT5,induces
R590	T764	T776	themeOf	NFAT5,expression
R591	T768	T767	themeOf	expression,silencing
R592	T770	T769	themeOf	expression,induced
R593	T774	T773	themeOf	expression,inhibits
R594	T774	T772	themeOf	expression,MTb-induced
R595	T776	T775	themeOf	expression,induces
R2034	T2670	T2687	themeOf	TLR2,Engagement
R2043	T2671	T2686	themeOf	MyD88,recruitment
R2047	T2671	T2686	themeOf	MyD88,recruitment
R2050	T2671	T2686	themeOf	MyD88,recruitment
R2057	T2672	T2686	themeOf	IRAK1,recruitment
R2064	T2673	T2686	themeOf	IRAK4,recruitment
R2069	T2673	T2688	themeOf	IRAK4,engagement
R2075	T2674	T2686	themeOf	TRAF6,recruitment
R2076	T2676	T2689	themeOf	NFAT5,binds
R2077	T2677	T2689	themeOf	TonEBP,binds
R2080	T2678	T2690	themeOf	NFAT5,activated
R2084	T2682	T2691	themeOf	NFAT5,expression
R2090	T2684	T2693	themeOf	NFAT5,expression
R2096	T2691	T2692	themeOf	expression,increases

BioNLP16_Messiy

Id	Subject	Object	Predicate	Lexical cue
T7006	16397-16400	Protein	denotes	TNF
T7005	16081-16091	Protein	denotes	luciferase
T7004	15660-15670	Protein	denotes	luciferase
T6597	15033-15040	Protein	denotes	β-actin
T6243	14428-14431	Protein	denotes	BSA
T6242	14105-14108	Protein	denotes	BSA
T5321	11260-11270	Gene_expression	denotes	expressing
T3808	8772-8782	Protein	denotes	luciferase
T3182	7201-7208	Negative_regulation	denotes	lacking
T3181	7305-7309	Protein	denotes	CD14
T3180	7209-7213	Protein	denotes	MCSF
T3179	6988-6992	Protein	denotes	MCSF
T3178	6735-6739	Protein	denotes	CD14
T1874	5927-5937	Gene_expression	denotes	expression
T1873	5793-5802	Positive_regulation	denotes	increases
T1872	5814-5824	Gene_expression	denotes	expression
T1871	5628-5635	Binding	denotes	binding
T1870	5328-5337	Positive_regulation	denotes	activated
T1869	5444-5452	Positive_regulation	denotes	involved
T1868	4905-4910	Binding	denotes	binds
T1867	3707-3717	Binding	denotes	engagement
T1866	3677-3687	Binding	denotes	Engagement
T1865	3766-3777	Binding	denotes	recruitment
T1864	6003-6008	Protein	denotes	NFAT5
T1863	5921-5926	Protein	denotes	NFAT5
T1862	5849-5854	Protein	denotes	MyD88
T1861	5803-5808	Protein	denotes	NFAT5
T1860	5606-5611	Protein	denotes	NFAT5
T1859	5426-5431	Protein	denotes	NFAT5
T1858	5384-5389	Protein	denotes	MyD88
T1857	5272-5277	Protein	denotes	NFAT5
T1856	4896-4902	Protein	denotes	TonEBP
T1853	3850-3855	Protein	denotes	TRAF6
T441	94-99	Protein	denotes	NFAT5
T1855	4875-4880	Protein	denotes	NFAT5
T1854	3893-3898	Protein	denotes	TRAF6
T1852	3818-3823	Protein	denotes	IRAK4
T1851	3808-3813	Protein	denotes	IRAK1
T1850	3741-3746	Protein	denotes	MyD88
T1849	3691-3695	Protein	denotes	TLR2
T1848	3639-3665	Protein	denotes	toll-like receptor (TLR) 2
T1847	3284-3287	Protein	denotes	CD4
T1846	3053-3056	Protein	denotes	CD4
T442	497-534	Protein	denotes	nuclear factor of activated T cells 5
T443	536-541	Protein	denotes	NFAT5
T444	682-687	Protein	denotes	NFAT5
T445	1106-1111	Protein	denotes	MyD88
T446	1113-1118	Protein	denotes	IRAK1
T447	1124-1129	Protein	denotes	TRAF6
T448	1166-1171	Protein	denotes	NFAT5
T449	1286-1291	Protein	denotes	NFAT5
T450	1247-1252	Protein	denotes	NFAT5
T451	1453-1458	Protein	denotes	NFAT5
T452	458-468	Gene_expression	denotes	expression
T453	441-450	Negative_regulation	denotes	silencing
T454	729-736	Positive_regulation	denotes	induced
T455	705-715	Gene_expression	denotes	expression
T456	912-920	Positive_regulation	denotes	required
T457	934-943	Positive_regulation	denotes	induction
T458	1177-1187	Gene_expression	denotes	expression
T459	1145-1153	Negative_regulation	denotes	inhibits
T460	1154-1165	Positive_regulation	denotes	MTb-induced
T461	1270-1280	Gene_expression	denotes	expression
T462	1365-1376	Binding	denotes	interaction
T463	1239-1246	Positive_regulation	denotes	induces
T4835	10609-10618	Regulation	denotes	targeting
T4834	10603-10608	Negative_regulation	denotes	siRNA
T4833	11126-11131	Protein	denotes	NFAT5
T4832	10650-10653	Protein	denotes	GFP
T4831	10623-10648	Protein	denotes	green fluorescent protein
T4830	10470-10475	Protein	denotes	NFAT5
T4463	9983-9987	Protein	denotes	CCR5
T4462	9893-9898	Protein	denotes	CXCR4
T4461	9826-9831	Protein	denotes	gp160
T4188	9265-9274	Gene_expression	denotes	expressed
T4187	9492-9499	Protein	denotes	DNase I
T4186	9423-9426	Protein	denotes	p65
T4185	9415-9418	Protein	denotes	p50
T4184	9203-9208	Protein	denotes	NFAT5
T4183	9170-9177	Protein	denotes	DNase I
T3811	9026-9036	Gene_expression	denotes	expression
T3810	9061-9071	Protein	denotes	luciferase
T3809	9012-9025	Protein	denotes	Reporter gene
T5320	11213-11223	Gene_expression	denotes	expressing
T5319	11801-11806	Protein	denotes	TRAF6
T5318	11638-11643	Protein	denotes	TRAF6
T5317	11586-11591	Protein	denotes	IRAK1
T5316	11418-11423	Protein	denotes	IRAK1
T5315	11293-11298	Protein	denotes	MyD88
T5314	11271-11276	Protein	denotes	shRNA
T5313	11224-11229	Protein	denotes	shRNA
T4837	11132-11142	Gene_expression	denotes	expression
T4836	11118-11122	Negative_regulation	denotes	down
T21662	18805-18814	Positive_regulation	denotes	increases
T21661	18622-18632	Gene_expression	denotes	expression
T21660	18243-18250	Binding	denotes	binding
T21659	18223-18233	Negative_regulation	denotes	Disruption
T21658	17967-17977	Gene_expression	denotes	expression
T21657	16477-16488	Binding	denotes	interaction
T21654	18815-18820	Protein	denotes	NFAT5
T21653	18603-18613	Protein	denotes	luciferase
T21652	18237-18242	Protein	denotes	NFAT5
T21651	17956-17966	Protein	denotes	luciferase
T21650	16893-16903	Protein	denotes	luciferase
T21649	16471-16476	Protein	denotes	NFAT5
R1375	T1849	T1866	themeOf	TLR2,Engagement
R1376	T1850	T1865	themeOf	MyD88,recruitment
R1377	T1850	T1865	themeOf	MyD88,recruitment
R1378	T1850	T1865	themeOf	MyD88,recruitment
R1379	T1850	T1867	themeOf	MyD88,engagement
R1380	T1851	T1865	themeOf	IRAK1,recruitment
R1381	T1852	T1865	themeOf	IRAK4,recruitment
R1382	T1853	T1865	themeOf	TRAF6,recruitment
R1388	T1861	T1872	themeOf	NFAT5,expression
R1389	T1863	T1874	themeOf	NFAT5,expression
R1390	T1872	T1873	themeOf	expression,increases
R2474	T3180	T3182	themeOf	MCSF,lacking
R3022	T3809	T3811	themeOf	Reporter gene,expression
R3308	T4184	T4188	themeOf	NFAT5,expressed
R3797	T4831	T4835	themeOf	green fluorescent protein,targeting
R3798	T4832	T4835	themeOf	GFP,targeting
R3799	T4833	T4837	themeOf	NFAT5,expression
R3800	T4835	T4834	themeOf	targeting,siRNA
R3801	T4835	T4834	themeOf	targeting,siRNA
R3802	T4837	T4836	themeOf	expression,down
R4182	T5313	T5320	themeOf	shRNA,expressing
R4183	T5315	T5321	themeOf	MyD88,expressing
R15979	T21649	T21657	themeOf	NFAT5,interaction
R15980	T21651	T21658	themeOf	luciferase,expression
R15981	T21652	T21659	themeOf	NFAT5,Disruption
R15982	T21652	T21660	themeOf	NFAT5,binding
R15983	T21653	T21661	themeOf	luciferase,expression
R15984	T21654	T21662	themeOf	NFAT5,increases
R15985	T21660	T21659	themeOf	binding,Disruption
R508	T443	T452	themeOf	NFAT5,expression
R509	T444	T454	themeOf	NFAT5,induced
R510	T444	T457	themeOf	NFAT5,induction
R511	T444	T455	themeOf	NFAT5,expression
R512	T448	T458	themeOf	NFAT5,expression
R513	T449	T462	themeOf	NFAT5,interaction
R514	T450	T463	themeOf	NFAT5,induces
R515	T450	T461	themeOf	NFAT5,expression
R516	T450	T462	themeOf	NFAT5,interaction
R517	T452	T453	themeOf	expression,silencing
R518	T455	T454	themeOf	expression,induced
R519	T457	T456	themeOf	induction,required
R520	T458	T459	themeOf	expression,inhibits
R521	T458	T460	themeOf	expression,MTb-induced
R522	T461	T463	themeOf	expression,induces
R1383	T1855	T1868	themeOf	NFAT5,binds
R1384	T1856	T1868	themeOf	TonEBP,binds
R1385	T1857	T1870	themeOf	NFAT5,activated
R1386	T1859	T1869	themeOf	NFAT5,involved
R1387	T1860	T1871	themeOf	NFAT5,binding

DLUT931

Id	Subject	Object	Predicate	Lexical cue
T21636	18805-18814	Positive_regulation	denotes	increases
T21635	18622-18632	Gene_expression	denotes	expression
T21634	18243-18250	Binding	denotes	binding
T21633	18223-18233	Negative_regulation	denotes	Disruption
T21632	17967-17977	Gene_expression	denotes	expression
T21631	16477-16488	Binding	denotes	interaction
T21628	18815-18820	Protein	denotes	NFAT5
T21627	18603-18613	Protein	denotes	luciferase
T21626	18237-18242	Protein	denotes	NFAT5
T21625	17956-17966	Protein	denotes	luciferase
T21624	16893-16903	Protein	denotes	luciferase
T21623	16471-16476	Protein	denotes	NFAT5
T7003	16066-16077	Positive_regulation	denotes	enhancement
T7002	16397-16400	Protein	denotes	TNF
T7001	16081-16091	Protein	denotes	luciferase
T7000	15660-15670	Protein	denotes	luciferase
T6596	15041-15045	Transcription	denotes	mRNA
T6595	15033-15040	Protein	denotes	β-actin
T6241	14428-14431	Protein	denotes	BSA
T6240	14105-14108	Protein	denotes	BSA
T5312	11260-11270	Gene_expression	denotes	expressing
T5311	11213-11223	Gene_expression	denotes	expressing
T5310	11801-11806	Protein	denotes	TRAF6
T5309	11638-11643	Protein	denotes	TRAF6
T5308	11586-11591	Protein	denotes	IRAK1
T5307	11418-11423	Protein	denotes	IRAK1
T5306	11293-11298	Protein	denotes	MyD88
T5305	11271-11276	Protein	denotes	shRNA
T5304	11224-11229	Protein	denotes	shRNA
T4829	11118-11122	Negative_regulation	denotes	down
T4828	11132-11142	Gene_expression	denotes	expression
T4827	10609-10618	Negative_regulation	denotes	targeting
T4826	10603-10608	Negative_regulation	denotes	siRNA
T4825	11126-11131	Protein	denotes	NFAT5
T4824	10650-10653	Protein	denotes	GFP
T4823	10623-10648	Protein	denotes	green fluorescent protein
T4822	10470-10475	Protein	denotes	NFAT5
T4460	9797-9806	Negative_regulation	denotes	replacing
T4459	9983-9987	Protein	denotes	CCR5
T4458	9893-9898	Protein	denotes	CXCR4
T4457	9826-9831	Protein	denotes	gp160
T4182	9265-9274	Gene_expression	denotes	expressed
T4181	9492-9499	Protein	denotes	DNase I
T4180	9423-9426	Protein	denotes	p65
T4179	9415-9418	Protein	denotes	p50
T4178	9203-9208	Protein	denotes	NFAT5
T4177	9170-9177	Protein	denotes	DNase I
T3807	9026-9036	Gene_expression	denotes	expression
T3806	9061-9071	Protein	denotes	luciferase
T3805	9012-9025	Protein	denotes	Reporter gene
T3804	8772-8782	Protein	denotes	luciferase
T3177	7201-7208	Negative_regulation	denotes	lacking
T3176	7305-7309	Protein	denotes	CD14
T3175	7209-7213	Protein	denotes	MCSF
T3174	6988-6992	Protein	denotes	MCSF
T3173	6735-6739	Protein	denotes	CD14
T1823	5927-5937	Gene_expression	denotes	expression
T1822	5793-5802	Positive_regulation	denotes	increases
T1821	5814-5824	Gene_expression	denotes	expression
T1820	5628-5635	Binding	denotes	binding
T1819	5444-5452	Positive_regulation	denotes	involved
T1818	5328-5337	Positive_regulation	denotes	activated
T1817	4905-4910	Binding	denotes	binds
T1816	3766-3777	Binding	denotes	recruitment
T1815	3707-3717	Binding	denotes	engagement
T1814	3677-3687	Binding	denotes	Engagement
T1813	6003-6008	Protein	denotes	NFAT5
T1812	5921-5926	Protein	denotes	NFAT5
T1811	5849-5854	Protein	denotes	MyD88
T1810	5803-5808	Protein	denotes	NFAT5
T1809	5606-5611	Protein	denotes	NFAT5
T1808	5426-5431	Protein	denotes	NFAT5
T1807	5384-5389	Protein	denotes	MyD88
T1806	5272-5277	Protein	denotes	NFAT5
T1805	4896-4902	Protein	denotes	TonEBP
T1804	4875-4880	Protein	denotes	NFAT5
T1803	3893-3898	Protein	denotes	TRAF6
T1802	3850-3855	Protein	denotes	TRAF6
T1801	3818-3823	Protein	denotes	IRAK4
T1800	3808-3813	Protein	denotes	IRAK1
T1799	3741-3746	Protein	denotes	MyD88
T1798	3691-3695	Protein	denotes	TLR2
T1797	3639-3665	Protein	denotes	toll-like receptor (TLR) 2
T1796	3284-3287	Protein	denotes	CD4
T1795	3053-3056	Protein	denotes	CD4
T440	1239-1246	Positive_regulation	denotes	induces
T439	1365-1376	Binding	denotes	interaction
T438	1270-1280	Gene_expression	denotes	expression
T437	1145-1153	Negative_regulation	denotes	inhibits
T436	1154-1165	Positive_regulation	denotes	MTb-induced
T435	1177-1187	Gene_expression	denotes	expression
T434	729-736	Positive_regulation	denotes	induced
T421	94-99	Protein	denotes	NFAT5
T422	497-534	Protein	denotes	nuclear factor of activated T cells 5
T423	536-541	Protein	denotes	NFAT5
T424	682-687	Protein	denotes	NFAT5
T425	1106-1111	Protein	denotes	MyD88
T426	1113-1118	Protein	denotes	IRAK1
T427	1124-1129	Protein	denotes	TRAF6
T428	1166-1171	Protein	denotes	NFAT5
T429	1286-1291	Protein	denotes	NFAT5
T430	1247-1252	Protein	denotes	NFAT5
T431	1453-1458	Protein	denotes	NFAT5
T432	458-468	Gene_expression	denotes	expression
T433	705-715	Gene_expression	denotes	expression
R1367	T1821	T1822	themeOf	expression,increases
R2473	T3175	T3177	themeOf	MCSF,lacking
R3021	T3805	T3807	themeOf	Reporter gene,expression
R3307	T4178	T4182	themeOf	NFAT5,expressed
R3508	T4457	T4460	themeOf	gp160,replacing
R241	T435	T436	themeOf	expression,MTb-induced
R242	T436	T437	themeOf	MTb-induced,inhibits
R243	T438	T440	themeOf	expression,induces
R500	T422	T432	themeOf	nuclear factor of activated T cells 5,expression
R501	T423	T432	themeOf	NFAT5,expression
R502	T424	T433	themeOf	NFAT5,expression
R503	T424	T434	themeOf	NFAT5,induced
R504	T428	T435	themeOf	NFAT5,expression
R505	T429	T439	themeOf	NFAT5,interaction
R506	T430	T438	themeOf	NFAT5,expression
R507	T433	T434	themeOf	expression,induced
R1352	T1798	T1814	themeOf	TLR2,Engagement
R1353	T1799	T1815	themeOf	MyD88,engagement
R1354	T1799	T1816	themeOf	MyD88,recruitment
R1355	T1799	T1816	themeOf	MyD88,recruitment
R1356	T1799	T1816	themeOf	MyD88,recruitment
R1357	T1800	T1816	themeOf	IRAK1,recruitment
R1358	T1801	T1816	themeOf	IRAK4,recruitment
R1359	T1802	T1816	themeOf	TRAF6,recruitment
R1360	T1804	T1817	themeOf	NFAT5,binds
R1361	T1805	T1817	themeOf	TonEBP,binds
R1362	T1806	T1818	themeOf	NFAT5,activated
R1363	T1808	T1819	themeOf	NFAT5,involved
R1364	T1809	T1820	themeOf	NFAT5,binding
R1365	T1810	T1821	themeOf	NFAT5,expression
R1366	T1812	T1823	themeOf	NFAT5,expression
R3790	T4823	T4826	themeOf	green fluorescent protein,siRNA
R3791	T4823	T4827	themeOf	green fluorescent protein,targeting
R3792	T4824	T4827	themeOf	GFP,targeting
R3793	T4825	T4828	themeOf	NFAT5,expression
R3794	T4827	T4826	themeOf	targeting,siRNA
R3795	T4827	T4826	themeOf	targeting,siRNA
R3796	T4828	T4829	themeOf	expression,down
R4179	T5304	T5311	themeOf	shRNA,expressing
R4180	T5305	T5312	themeOf	shRNA,expressing
R4181	T5306	T5312	themeOf	MyD88,expressing
R5288	T6595	T6596	themeOf	β-actin,mRNA
R5631	T7001	T7003	themeOf	luciferase,enhancement
R15968	T21623	T21631	themeOf	NFAT5,interaction
R15969	T21625	T21632	themeOf	luciferase,expression
R15970	T21626	T21633	themeOf	NFAT5,Disruption
R15971	T21626	T21634	themeOf	NFAT5,binding
R15972	T21627	T21635	themeOf	luciferase,expression
R15973	T21628	T21636	themeOf	NFAT5,increases
R15974	T21634	T21633	themeOf	binding,Disruption

bionlp-st-ge-2016-test-ihmc

Id	Subject	Object	Predicate	Lexical cue
T22309	18079-18120	Binding	denotes	the NFAT5 binding site mutations (N5-Mut)
T22308	18223-18250	Negative_regulation	denotes	Disruption of NFAT5 binding
T22307	17791-17816	Binding	denotes	of the NFAT5 binding site
T22306	16556-16571	Positive_regulation	denotes	MTb stimulation
T22305	17771-17920	Negative_regulation	denotes	Specific disruption of the NFAT5 binding site significantly reduces LTR-reporter gene activity in monocytic cells in response to MTb lysate treatment
T22304	18595-18632	Regulation	denotes	Renilla luciferase control expression
T22303	16471-16501	Binding	denotes	NFAT5 interaction with the LTR
T22302	16519-16530	Protein	denotes	MTb-induced
T22301	18411-18413	Entity	denotes	p<
T22300	17956-17966	Protein	denotes	luciferase
T22298	17326-17351	Entity	denotes	MTb lysate-treated cells,
T22297	18456-18475	Entity	denotes	the untreated cells
T22296	17995-18006	Entity	denotes	nucleotides
T22295	18815-18820	Protein	denotes	NFAT5
T22294	18921-18924	Protein	denotes	MTb
T22293	17039-17042	Protein	denotes	MTb
T22292	18113-18119	Entity	denotes	N5-Mut
T22291	18138-18147	Entity	denotes	the cells
T22290	17432-17461	Entity	denotes	cells treated with MTb lysate
T22289	16781-16793	Protein	denotes	plasmid pGL3
T22288	18658-18668	Entity	denotes	Nucleotide
T22286	16881-16903	Protein	denotes	the Renilla luciferase
T22285	18182-18194	Protein	denotes	10 µg/ml MTb
T22284	16556-16559	Protein	denotes	MTb
T22283	17451-17454	Protein	denotes	MTb
T22282	18375-18380	Entity	denotes	cells
T22281	17869-17884	Entity	denotes	monocytic cells
T22280	17259-17274	Entity	denotes	untreated cells
T22279	18079-18120	Entity	denotes	the NFAT5 binding site mutations (N5-Mut)
T22278	18603-18613	Protein	denotes	luciferase
T22277	18839-18854	Entity	denotes	monocytic cells
T22276	18083-18088	Protein	denotes	NFAT5
T22275	17798-17803	Protein	denotes	NFAT5
T22273	18234-18242	Protein	denotes	of NFAT5
T22272	17900-17903	Protein	denotes	MTb
T22270	17165-17180	Entity	denotes	untreated cells
T22268	18815-18854	Protein	denotes	NFAT5 protein levels in monocytic cells
T22267	18710-18715	Protein	denotes	NFAT5
T22266	18113-18119	Protein	denotes	N5-Mut
T22265	17852-17856	Protein	denotes	gene
T22264	18959-18978	Entity	denotes	Whole cell extracts
T22263	16703-16707	Entity	denotes	site
T22262	17587-17590	Protein	denotes	MTb
T22261	16471-16476	Protein	denotes	NFAT5
T22260	17791-17816	Entity	denotes	of the NFAT5 binding site
T22259	18399-18402	Protein	denotes	MTb
T22258	18794-18797	Protein	denotes	MTb
T22257	17709-17727	Entity	denotes	p<0.05; **, p<0.01
T22256	16912-16939	Protein	denotes	plasmid pRL-TK (0.03 µg/ml)
T22255	17326-17329	Protein	denotes	MTb
T22254	16976-16981	Entity	denotes	Cells
T6674	14767-14793	Transcription	denotes	The mRNA expression levels
T6753	15230-15296	Entity	denotes	the paired Student t-Test with the aid of Microsoft Excel software
T6673	14767-14793	Gene_expression	denotes	The mRNA expression levels
T6672	14771-14775	Protein	denotes	mRNA
T6671	14750-14766	Protein	denotes	Quantitative PCR
T6670	14810-14891	Protein	denotes	by SYBR Green-based real-time PCR (Applied Biosystems, www.appliedbiosystems.com)
T6669	15033-15045	Protein	denotes	β-actin mRNA
T6668	15033-15045	Protein	denotes	β-actin mRNA
T7251	15439-15457	Positive_regulation	denotes	of MTb stimulation
T7250	15638-15684	Binding	denotes	linked to the firefly luciferase reporter gene
T7249	15442-15445	Protein	denotes	MTb
T7248	15343-15346	Protein	denotes	MTb
T7247	15950-15953	Protein	denotes	MTb
T7246	16081-16091	Protein	denotes	luciferase
T7245	15847-15850	Protein	denotes	MTb
T7244	15892-15901	Entity	denotes	monocytes
T7243	15821-15832	Protein	denotes	MTb (H37Rv)
T7242	15645-15684	Protein	denotes	to the firefly luciferase reporter gene
T7241	16397-16400	Protein	denotes	TNF
T7240	15826-15829	Entity	denotes	H37
T7239	15652-15670	Protein	denotes	firefly luciferase
T6520	14692-14747	Entity	denotes	SuperSignal West Pico Chemiluminescent Reagent (Pierce)
T6519	14088-14098	Entity	denotes	a solution
T6518	14358-14388	Protein	denotes	of goat anti-Lamin-B1 antibody
T6517	14441-14464	Entity	denotes	2 h at room temperature
T6516	13830-13838	Entity	denotes	Extracts
T6515	14272-14309	Entity	denotes	of rabbit anti-NFAT5 antibody (H-300)
T6514	14533-14573	Protein	denotes	anti-goat-HRP (Santa Cruz Biotechnology)
T6513	13695-13703	Entity	denotes	Tris–HCl
T6512	13676-13704	Entity	denotes	150 mM NaCl, 50 mM Tris–HCl,
T6511	13987-14041	Entity	denotes	a nitrocellulose Trans-Blot Transfer Membrane (BioRad)
T6510	13960-13967	Entity	denotes	The gel
T6509	13763-13772	Entity	denotes	EDTA-free
T6508	14311-14316	Protein	denotes	Santa
T6507	14167-14177	Entity	denotes	50 mM Tris
T6506	13695-13703	Entity	denotes	Tris–HCl
T6505	13923-13931	Protein	denotes	proteins
T6504	13713-13722	Entity	denotes	1% Triton
T6503	14182-14193	Entity	denotes	150 mM NaCl
T6502	13897-13918	Entity	denotes	v/v 2-mercaptoethanol
T6501	14073-14076	Entity	denotes	1 h
T6500	13754-13781	Protein	denotes	Complete EDTA-free Protease
T6499	14102-14116	Protein	denotes	4% BSA (Sigma)
T6498	14399-14404	Protein	denotes	Santa
T6497	13700-13703	Entity	denotes	HCl
T6496	14110-14115	Protein	denotes	Sigma
T6495	13724-13736	Entity	denotes	10% glycerol
T6494	13623-13631	Entity	denotes	extracts
T6493	13782-13791	Entity	denotes	Inhibitor
T6492	13987-14041	Entity	denotes	a nitrocellulose Trans-Blot Transfer Membrane (BioRad)
T6491	14205-14208	Protein	denotes	BSA
T6490	14004-14009	Protein	denotes	Trans
T5984	13572-13596	Regulation	denotes	infection of primary MDM
T5983	12825-12855	Entity	denotes	albumin dextrose complex (ADC)
T5982	12825-12855	Protein	denotes	albumin dextrose complex (ADC)
T5981	13582-13596	Protein	denotes	of primary MDM
T5980	12876-12881	Protein	denotes	Sigma
T5979	12825-12855	Entity	denotes	albumin dextrose complex (ADC)
T5978	12851-12854	Entity	denotes	ADC
T5977	13156-13180	Entity	denotes	a single cell suspension
T5976	13295-13299	Entity	denotes	cell
T5975	12664-12667	Protein	denotes	MTb
T5974	12785-12788	Protein	denotes	Dif
T5973	13252-13299	Entity	denotes	further dilution with RPMI 1640 medium for cell
T5972	12785-12790	Entity	denotes	Difco
T5971	12648-12651	Protein	denotes	MTb
T5034	11126-11142	Gene_expression	denotes	NFAT5 expression
T5033	11018-11023	Entity	denotes	cells
T5032	11067-11070	Protein	denotes	MDM
T5031	10761-10794	Entity	denotes	siPORT NeoFX transfection reagent
T5030	10564-10590	Protein	denotes	non-specific siRNA effects
T5029	11126-11131	Protein	denotes	NFAT5
T5028	10385-10388	Protein	denotes	RNA
T5027	10650-10653	Protein	denotes	GFP
T5026	10686-10689	Protein	denotes	MDM
T5025	10373-10379	Protein	denotes	of MDM
T5024	10623-10655	Protein	denotes	green fluorescent protein (GFP),
T5023	10466-10486	Protein	denotes	the NFAT5 transcript
T5022	10470-10475	Protein	denotes	NFAT5
T5576	11632-11722	Protein	denotes	human TRAF6 (forward primer 5′-CCGGAGAAACCTGTTGTGATTCGTCTCATAAATCACAACAGGTTTCTCCTTTTTG-3′,
T5575	11271-11298	Protein	denotes	shRNA targeting human MyD88
T5574	12626-12645	Entity	denotes	0.8 µg/ml puromycin
T5573	11287-11298	Protein	denotes	human MyD88
T5572	11801-11806	Protein	denotes	TRAF6
T5571	11511-11584	Protein	denotes	primer 5′-AATTCAAAAAGGAGCAGCTGTCCAGGTTTTATGAGACGAAACCTGGACAGCTGCT-3′ mRNA
T5570	12155-12175	Entity	denotes	transfection reagent
T5569	11224-11229	Protein	denotes	shRNA
T5568	11586-11591	Protein	denotes	IRAK1
T5567	12575-12580	Entity	denotes	cells
T4055	8950-8962	Protein	denotes	10 µg/ml MTb
T4054	8784-8790	Protein	denotes	pRL-TK
T4053	8813-8843	Entity	denotes	Effectene transfection reagent
T4052	8543-8549	Entity	denotes	N5-Mut
T4051	8516-8550	Entity	denotes	NFAT5 binding site-mutant (N5-Mut)
T4050	8399-8417	Protein	denotes	restriction enzyme
T4049	8764-8791	Protein	denotes	Renilla luciferase (pRL-TK)
T4048	8543-8549	Protein	denotes	N5-Mut
T4047	8390-8423	Entity	denotes	Hind III restriction enzyme sites
T4046	8072-8083	Entity	denotes	nucleotides
T4045	8870-8875	Entity	denotes	Cells
T4624	9983-9987	Protein	denotes	CCR5
T4623	10031-10035	Protein	denotes	CCR5
T4622	10148-10152	Entity	denotes	PBMC
T4621	9893-9898	Protein	denotes	CXCR4
T4620	9826-9843	Entity	denotes	gp160 amino acids
T4619	9686-9706	Entity	denotes	the NIH AIDS Reagent
T4618	9826-9831	Protein	denotes	gp160
T4617	10039-10059	Protein	denotes	a secondary receptor
T4616	9821-9824	Protein	denotes	env
T4615	10129-10142	Entity	denotes	DNA extracted
T4301	9403-9441	Binding	denotes	Recombinant p50 and p65 were purchased
T4300	9378-9401	Entity	denotes	Ni-NTA agarose (Qiagen)
T4299	9170-9177	Protein	denotes	DNase I
T4298	9191-9208	Protein	denotes	Recombinant NFAT5
T4297	9381-9384	Entity	denotes	NTA
T4296	9423-9426	Protein	denotes	p65
T4295	9403-9418	Protein	denotes	Recombinant p50
T4294	9479-9555	Protein	denotes	Quantitative DNase I footprinting was performed as previously described [31]
T4293	9236-9249	Entity	denotes	an N-terminal
T4292	9210-9221	Entity	denotes	amino acids
T4291	9278-9301	Entity	denotes	E. coli BL21(DE3) cells
T2885	6188-6229	Entity	denotes	peripheral blood mononuclear cells, PBMC)
T2884	6224-6228	Entity	denotes	PBMC
T2883	6264-6268	Protein	denotes	Bank
T3460	7249-7299	Binding	denotes	of the adherent cells obtained with this technique
T3459	7498-7547	Binding	denotes	293T cells were obtained from ATCC (www.atcc.org)
T3458	7247-7299	Entity	denotes	% of the adherent cells obtained with this technique
T3457	7158-7169	Entity	denotes	supernatant
T3456	6655-6670	Entity	denotes	Human monocytes
T3455	6498-6529	Entity	denotes	2 mM L-glutamine (BioWhittaker,
T3454	7305-7322	Entity	denotes	CD14+ macrophages
T3453	6685-6707	Entity	denotes	from PBMC preparations
T3452	6317-6353	Entity	denotes	PBMC from normal unidentified donors
T3451	7249-7299	Entity	denotes	of the adherent cells obtained with this technique
T3450	6852-6863	Entity	denotes	1×106 cells
T3449	6735-6739	Protein	denotes	CD14
T3448	7059-7079	Entity	denotes	Nabi, Boca Raton, FL
T3447	6964-6992	Protein	denotes	ng/ml recombinant human MCSF
T3446	7082-7099	Entity	denotes	The cell cultures
T3445	7209-7213	Protein	denotes	MCSF
T3444	7498-7508	Entity	denotes	293T cells
T3443	6756-6796	Protein	denotes	Miltenyi Biotec (www.miltenyibiotec.com)
T3442	6617-6653	Entity	denotes	Gemini Bio-Products, www.gembio.com)
T3441	6690-6694	Entity	denotes	PBMC
T3572	7915-7917	Entity	denotes	25
T3571	7813-7826	Entity	denotes	AIDS Reagents
T3570	7677-7685	Protein	denotes	HIV-1Bal
T2787	3930-3961	Positive_regulation	denotes	mitogen-activated protein (MAP)
T2786	2998-3011	Regulation	denotes	MTb infection
T2785	3574-3636	Localization	denotes	The primary PRR on monocytic cells triggered by MTb infection
T2784	3751-3855	Binding	denotes	the subsequent recruitment of several kinases, including IRAK1 and IRAK4, and the ubiquitin ligase TRAF6
T2783	4714-4757	Binding	denotes	NF-κB binding sites, respectively [1], [28]
T2782	3893-3969	Positive_regulation	denotes	TRAF6 activates IκB kinase (IKK) and mitogen-activated protein (MAP) kinases
T2781	3456-3483	Positive_regulation	denotes	of latent MTb reactivation,
T2780	5779-5792	Regulation	denotes	MTb infection
T2779	5064-5105	Localization	denotes	the core NF-κB binding motifs in the LTR
T2778	3619-3635	Regulation	denotes	by MTb infection
T2777	3574-3635	Positive_regulation	denotes	The primary PRR on monocytic cells triggered by MTb infection
T2776	4003-4046	Positive_regulation	denotes	activation of specific transcription factor
T2775	5384-5406	Positive_regulation	denotes	MyD88 signaling, [32],
T2774	5803-5843	Gene_expression	denotes	NFAT5 gene expression in human monocytes
T2773	5918-5937	Gene_expression	denotes	of NFAT5 expression
T2772	5759-5871	Positive_regulation	denotes	we demonstrate that MTb infection increases NFAT5 gene expression in human monocytes in a MyD88-dependent manner
T2771	4343-4369	Entity	denotes	of activator binding sites
T2770	4728-4733	Entity	denotes	sites
T2769	3574-3635	Protein	denotes	The primary PRR on monocytic cells triggered by MTb infection
T2768	2761-2765	Protein	denotes	PRRs
T2767	5803-5808	Protein	denotes	NFAT5
T2766	5456-5467	Protein	denotes	MTb-induced
T2765	2931-2940	Protein	denotes	cytokines
T2764	3829-3855	Protein	denotes	the ubiquitin ligase TRAF6
T2763	5743-5747	Entity	denotes	PBMC
T2762	2738-2766	Protein	denotes	recognition receptors (PRRs)
T2761	3466-3469	Protein	denotes	MTb
T2760	5064-5104	Protein	denotes	the core NF-κB binding motifs in the LTR
T2759	2998-3001	Protein	denotes	MTb
T2758	3284-3287	Protein	denotes	CD4
T2757	5044-5049	Protein	denotes	NFAT5
T2756	6036-6047	Protein	denotes	MTb-induced
T2755	5426-5431	Protein	denotes	NFAT5
T2754	3053-3056	Protein	denotes	CD4
T2753	3930-3961	Protein	denotes	mitogen-activated protein (MAP)
T2752	5601-5611	Protein	denotes	that NFAT5
T2751	3733-3855	Protein	denotes	protein MyD88 and the subsequent recruitment of several kinases, including IRAK1 and IRAK4, and the ubiquitin ligase TRAF6
T2750	4679-4693	Entity	denotes	southeast Asia
T2749	4081-4085	Protein	denotes	AP-1
T2748	3053-3064	Entity	denotes	CD4 T cells
T2747	4071-4076	Protein	denotes	NF-κB
T2746	2777-2799	Entity	denotes	mononuclear phagocytes
T2745	4911-4920	Entity	denotes	to a site
T2744	5250-5253	Protein	denotes	MDM
T2743	5544-5578	Entity	denotes	peripheral blood mononuclear cells
T2742	4714-4757	Protein	denotes	NF-κB binding sites, respectively [1], [28]
T2741	5849-5864	Protein	denotes	MyD88-dependent
T2740	5671-5692	Protein	denotes	efficient MTb-induced
T2739	3893-3898	Protein	denotes	TRAF6
T2738	3833-3842	Protein	denotes	ubiquitin
T2737	3691-3695	Protein	denotes	TLR2
T2736	4896-4902	Protein	denotes	TonEBP
T2735	5212-5228	Entity	denotes	primary monocyte
T2734	4364-4369	Entity	denotes	sites
T2733	5735-5738	Protein	denotes	MDM
T2732	5530-5539	Entity	denotes	monocytes
T2731	5272-5277	Protein	denotes	NFAT5
T2730	3781-3824	Protein	denotes	several kinases, including IRAK1 and IRAK4,
T2729	4014-4046	Protein	denotes	of specific transcription factor
T2728	3818-3823	Protein	denotes	IRAK4
T2727	2770-2773	Protein	denotes	MTb
T2726	5384-5389	Protein	denotes	MyD88
T2725	5068-5072	Entity	denotes	core
T2724	5338-5360	Protein	denotes	by the MAP kinase p38,
T2723	5921-5926	Protein	denotes	NFAT5
T2722	5039-5054	Entity	denotes	This NFAT5 site
T2721	5779-5782	Protein	denotes	MTb
T2720	6003-6008	Protein	denotes	NFAT5
T2719	3639-3670	Protein	denotes	toll-like receptor (TLR) 2 [16]
T2718	3284-3294	Entity	denotes	CD4 T cell
T2717	5636-5640	Entity	denotes	site
T2716	3079-3084	Entity	denotes	cells
T2715	3909-3925	Protein	denotes	IκB kinase (IKK)
T2714	3622-3625	Protein	denotes	MTb
T2713	2529-2532	Protein	denotes	MTb
T2712	5828-5843	Entity	denotes	human monocytes
T2711	5237-5254	Entity	denotes	macrophages (MDM)
T2710	3808-3813	Protein	denotes	IRAK1
T2709	2654-2697	Protein	denotes	human immunodeficiency virus type 1 (HIV-1)
T2708	3829-3855	Protein	denotes	the ubiquitin ligase TRAF6
T2707	3930-3947	Entity	denotes	mitogen-activated
T2706	5345-5348	Protein	denotes	MAP
T2705	2945-2964	Protein	denotes	chemokines produced
T2704	4852-4866	Entity	denotes	T cells (NFAT)
T2703	3909-3969	Protein	denotes	IκB kinase (IKK) and mitogen-activated protein (MAP) kinases
T2702	3957-3960	Protein	denotes	MAP
T2701	4875-4880	Protein	denotes	NFAT5
T2700	5338-5360	Protein	denotes	by the MAP kinase p38,
T2699	3741-3746	Protein	denotes	MyD88
T2698	3200-3211	Protein	denotes	MTb-induced
T2697	3593-3608	Entity	denotes	monocytic cells
T2696	4542-4547	Protein	denotes	NF-κB
T2695	5945-5957	Protein	denotes	PRR-mediated
T2694	5338-5360	Protein	denotes	by the MAP kinase p38,
T823	798-826	Binding	denotes	an intact NFAT5 binding site
T822	1225-1238	Regulation	denotes	MTb infection
T821	469-578	Negative_regulation	denotes	of the transcription factor nuclear factor of activated T cells 5 (NFAT5) by RNA interference (RNAi) inhibits
T820	1106-1187	Negative_regulation	denotes	MyD88, IRAK1, and TRAF6, significantly inhibits MTb-induced NFAT5 gene expression
T819	1471-1510	Regulation	denotes	MTb-mediated control of gene expression
T818	1262-1280	Gene_expression	denotes	protein expression
T817	1154-1187	Gene_expression	denotes	MTb-induced NFAT5 gene expression
T816	697-715	Gene_expression	denotes	protein expression
T815	1365-1400	Binding	denotes	interaction with the viral promoter
T814	1313-1400	Regulation	denotes	in MTb regulation of HIV-1 replication via a direct interaction with the viral promoter
T813	421-578	Negative_regulation	denotes	we demonstrate that silencing of the expression of the transcription factor nuclear factor of activated T cells 5 (NFAT5) by RNA interference (RNAi) inhibits
T812	451-578	Gene_expression	denotes	of the expression of the transcription factor nuclear factor of activated T cells 5 (NFAT5) by RNA interference (RNAi) inhibits
T811	1471-1510	Regulation	denotes	MTb-mediated control of gene expression
T810	737-743	Protein	denotes	by MTb
T809	682-692	Protein	denotes	NFAT5 gene
T808	1096-1105	Entity	denotes	molecules
T805	94-99	Protein	denotes	NFAT5
T798	103-125	Protein	denotes	the Toll-Like Receptor
T778	1247-1252	Protein	denotes	NFAT5
T779	1225-1228	Protein	denotes	MTb
T780	756-788	Entity	denotes	Toll-like receptor (TLR) ligand,
T781	1106-1130	Protein	denotes	MyD88, IRAK1, and TRAF6,
T782	830-875	Entity	denotes	the viral promoter of R5-tropic HIV-1 subtype
T783	1316-1319	Protein	denotes	MTb
T784	1247-1257	Protein	denotes	NFAT5 gene
T785	1377-1400	Entity	denotes	with the viral promoter
T786	644-667	Entity	denotes	co-infected macrophages
T787	965-971	Protein	denotes	by MTb
T788	808-813	Protein	denotes	NFAT5
T789	1471-1483	Protein	denotes	MTb-mediated
T790	798-826	Entity	denotes	an intact NFAT5 binding site
T791	1044-1060	Entity	denotes	human monocytes,
T792	1113-1119	Protein	denotes	IRAK1,
T793	1262-1269	Protein	denotes	protein
T794	1166-1171	Protein	denotes	NFAT5
T795	469-578	Protein	denotes	of the transcription factor nuclear factor of activated T cells 5 (NFAT5) by RNA interference (RNAi) inhibits
T796	756-780	Protein	denotes	Toll-like receptor (TLR)
T797	497-542	Protein	denotes	nuclear factor of activated T cells 5 (NFAT5)
T799	1286-1291	Protein	denotes	NFAT5
T800	682-687	Protein	denotes	NFAT5
T801	1154-1165	Protein	denotes	MTb-induced
T802	1124-1129	Protein	denotes	TRAF6
T803	536-541	Protein	denotes	NFAT5
T804	607-610	Protein	denotes	MTb
T806	697-704	Protein	denotes	protein
T807	1453-1458	Protein	denotes	NFAT5
T5592	11194-11229	Gene_expression	denotes	Stable THP-1 cells expressing shRNA
T5591	11194-11229	Transcription	denotes	Stable THP-1 cells expressing shRNA
T5590	11592-11596	Protein	denotes	mRNA
T5589	11938-11943	Protein	denotes	shRNA
T5588	12394-12421	Entity	denotes	supernatants from the virus
T5587	12341-12365	Entity	denotes	the lentiviral particles
T5586	12102-12106	Protein	denotes	PAX2
T5585	11396-11401	Protein	denotes	shRNA
T5584	12465-12474	Entity	denotes	polybrene
T5583	12379-12388	Entity	denotes	the cells
T5582	11396-11502	Protein	denotes	shRNA targeting human IRAK1 (forward primer 5′-CCGGAGCAGCTGTCCAGGTTTCGTCTCATAAAACCTGGACAGCTGCTCCTTTTTG-3′,
T5581	11194-11229	Entity	denotes	Stable THP-1 cells expressing shRNA
T5580	12202-12214	Entity	denotes	Supernatants
T5579	12432-12437	Entity	denotes	cells
T5578	12023-12041	Protein	denotes	the shRNA-encoding
T5577	11807-11811	Protein	denotes	mRNA
R603	T795	T812	themeOf	of the transcription factor nuclear factor of activated T cells 5 (NFAT5) by RNA interference (RNAi) inhibits,of the expression of the transcription factor nuclear factor of activated T cells 5 (NFAT5) by RNA interference (RNAi) inhibits
R604	T795	T821	themeOf	of the transcription factor nuclear factor of activated T cells 5 (NFAT5) by RNA interference (RNAi) inhibits,of the transcription factor nuclear factor of activated T cells 5 (NFAT5) by RNA interference (RNAi) inhibits
R605	T797	T821	causeOf	nuclear factor of activated T cells 5 (NFAT5),of the transcription factor nuclear factor of activated T cells 5 (NFAT5) by RNA interference (RNAi) inhibits
R606	T806	T816	themeOf	protein,protein expression
R2107	T2697	T2785	locationOf	monocytic cells,The primary PRR on monocytic cells triggered by MTb infection
R2120	T2703	T2782	themeOf	IκB kinase (IKK) and mitogen-activated protein (MAP) kinases,TRAF6 activates IκB kinase (IKK) and mitogen-activated protein (MAP) kinases
R2134	T2707	T2787	causeOf	mitogen-activated,mitogen-activated protein (MAP)
R2142	T2714	T2778	themeOf	MTb,by MTb infection
R2143	T2721	T2780	themeOf	MTb,MTb infection
R2144	T2723	T2773	themeOf	NFAT5,of NFAT5 expression
R2145	T2725	T2779	locationOf	core,the core NF-κB binding motifs in the LTR
R2146	T2726	T2775	causeOf	MyD88,"MyD88 signaling, [32],"
R2147	T2729	T2776	themeOf	of specific transcription factor,activation of specific transcription factor
R2148	T2730	T2784	themeOf	"several kinases, including IRAK1 and IRAK4,","the subsequent recruitment of several kinases, including IRAK1 and IRAK4, and the ubiquitin ligase TRAF6"
R2149	T2739	T2782	causeOf	TRAF6,TRAF6 activates IκB kinase (IKK) and mitogen-activated protein (MAP) kinases
R2150	T2742	T2783	themeOf	"NF-κB binding sites, respectively [1], [28]","NF-κB binding sites, respectively [1], [28]"
R2151	T2753	T2787	themeOf	mitogen-activated protein (MAP),mitogen-activated protein (MAP)
R2152	T2759	T2786	themeOf	MTb,MTb infection
R2153	T2760	T2779	themeOf	the core NF-κB binding motifs in the LTR,the core NF-κB binding motifs in the LTR
R2154	T2761	T2781	themeOf	MTb,"of latent MTb reactivation,"
R2155	T2764	T2784	themeOf	the ubiquitin ligase TRAF6,"the subsequent recruitment of several kinases, including IRAK1 and IRAK4, and the ubiquitin ligase TRAF6"
R2156	T2767	T2774	themeOf	NFAT5,NFAT5 gene expression in human monocytes
R2157	T2769	T2777	themeOf	The primary PRR on monocytic cells triggered by MTb infection,The primary PRR on monocytic cells triggered by MTb infection
R2158	T2769	T2785	themeOf	The primary PRR on monocytic cells triggered by MTb infection,The primary PRR on monocytic cells triggered by MTb infection
R2159	T2774	T2772	themeOf	NFAT5 gene expression in human monocytes,we demonstrate that MTb infection increases NFAT5 gene expression in human monocytes in a MyD88-dependent manner
R2160	T2778	T2777	causeOf	by MTb infection,The primary PRR on monocytic cells triggered by MTb infection
R2161	T2780	T2772	causeOf	MTb infection,we demonstrate that MTb infection increases NFAT5 gene expression in human monocytes in a MyD88-dependent manner
R3390	T4295	T4301	themeOf	Recombinant p50,Recombinant p50 and p65 were purchased
R3391	T4296	T4301	themeOf	p65,Recombinant p50 and p65 were purchased
R3971	T5029	T5034	themeOf	NFAT5,NFAT5 expression
R4408	T5569	T5591	themeOf	shRNA,Stable THP-1 cells expressing shRNA
R4409	T5569	T5592	themeOf	shRNA,Stable THP-1 cells expressing shRNA
R4770	T5981	T5984	themeOf	of primary MDM,infection of primary MDM
R5355	T6672	T6673	themeOf	mRNA,The mRNA expression levels
R5356	T6672	T6674	themeOf	mRNA,The mRNA expression levels
R5840	T7242	T7250	themeOf	to the firefly luciferase reporter gene,linked to the firefly luciferase reporter gene
R5841	T7249	T7251	themeOf	MTb,of MTb stimulation
R16544	T22260	T22307	themeOf	of the NFAT5 binding site,of the NFAT5 binding site
R16545	T22261	T22303	themeOf	NFAT5,NFAT5 interaction with the LTR
R16546	T22265	T22305	themeOf	gene,Specific disruption of the NFAT5 binding site significantly reduces LTR-reporter gene activity in monocytic cells in response to MTb lysate treatment
R16547	T22273	T22308	themeOf	of NFAT5,Disruption of NFAT5 binding
R16548	T22278	T22304	themeOf	luciferase,Renilla luciferase control expression
R16549	T22284	T22306	themeOf	MTb,MTb stimulation
R454	T811	T819	themeOf	MTb-mediated control of gene expression,MTb-mediated control of gene expression
R455	T812	T813	themeOf	of the expression of the transcription factor nuclear factor of activated T cells 5 (NFAT5) by RNA interference (RNAi) inhibits,we demonstrate that silencing of the expression of the transcription factor nuclear factor of activated T cells 5 (NFAT5) by RNA interference (RNAi) inhibits
R456	T815	T814	themeOf	interaction with the viral promoter,in MTb regulation of HIV-1 replication via a direct interaction with the viral promoter
R457	T817	T820	themeOf	MTb-induced NFAT5 gene expression,"MyD88, IRAK1, and TRAF6, significantly inhibits MTb-induced NFAT5 gene expression"
R596	T779	T822	themeOf	MTb,MTb infection
R597	T781	T820	causeOf	"MyD88, IRAK1, and TRAF6,","MyD88, IRAK1, and TRAF6, significantly inhibits MTb-induced NFAT5 gene expression"
R598	T785	T815	themeOf	with the viral promoter,interaction with the viral promoter
R599	T789	T819	causeOf	MTb-mediated,MTb-mediated control of gene expression
R600	T790	T823	themeOf	an intact NFAT5 binding site,an intact NFAT5 binding site
R601	T793	T818	themeOf	protein,protein expression
R602	T794	T817	themeOf	NFAT5,MTb-induced NFAT5 gene expression

bionlp-st-ge-2016-spacy-parsed

Id	Subject	Object	Predicate	Lexical cue
T515	0-10	NN	denotes	Regulation
T516	11-27	NNP	denotes	of Mycobacterium
T517	28-50	NNP	denotes	tuberculosis-Dependent
T518	51-56	NNP	denotes	HIV-1
T519	57-70	NNP	denotes	Transcription
T520	71-78	VBZ	denotes	Reveals
T521	79-80	DT	denotes	a
T522	81-84	NNP	denotes	New
T523	85-89	NNP	denotes	Role
T524	90-93	IN	denotes	for
T525	94-99	NNP	denotes	NFAT5
T526	100-102	IN	denotes	in
T527	103-106	DT	denotes	the
T528	107-116	NNP	denotes	Toll-Like
T529	117-125	NNP	denotes	Receptor
T530	126-133	NNP	denotes	Pathway
T22162	18898-18900	CC	denotes	or
T22161	18896-18897	-RRB-	denotes	)
T22160	18889-18896	NN	denotes	control
T22159	18888-18889	-LRB-	denotes	(
T22158	18878-18887	JJ	denotes	untreated
T22157	18873-18877	VBN	denotes	left
T22156	18868-18872	VBD	denotes	were
T22155	18862-18867	NNS	denotes	cells
T22154	18856-18861	CD	denotes	THP-1
T22153	18854-18855	.	denotes	.
T22152	18849-18854	NNS	denotes	cells
T22151	18839-18848	JJ	denotes	monocytic
T22150	18836-18838	IN	denotes	in
T22149	18829-18835	NNS	denotes	levels
T22148	18821-18828	NN	denotes	protein
T22147	18815-18820	CD	denotes	NFAT5
T22146	18805-18814	VBZ	denotes	increases
T22145	18798-18804	NN	denotes	lysate
T22144	18794-18797	NNP	denotes	MTb
T22143	18792-18793	-RRB-	denotes	)
T22142	18791-18792	NNP	denotes	C
T22141	18790-18791	-LRB-	denotes	(
T22140	18788-18789	.	denotes	.
T22139	18782-18788	NN	denotes	figure
T22138	18778-18781	DT	denotes	the
T22137	18775-18777	IN	denotes	of
T22136	18768-18774	NN	denotes	bottom
T22135	18764-18767	DT	denotes	the
T22134	18761-18763	IN	denotes	at
T22133	18755-18760	VBN	denotes	shown
T22132	18751-18754	VBP	denotes	are
T22131	18746-18750	NNS	denotes	LTRs
T22130	18737-18745	JJ	denotes	HIV-1Lai
T22129	18724-18736	JJ	denotes	site-mutated
T22128	18716-18723	JJ	denotes	binding
T22127	18710-18715	CD	denotes	NFAT5
T22126	18706-18709	CC	denotes	and
T22125	18696-18705	JJ	denotes	wild-type
T22124	18692-18695	DT	denotes	the
T22123	18679-18691	VBG	denotes	representing
T22122	18669-18678	NNS	denotes	sequences
T22121	18658-18668	NNP	denotes	Nucleotide
T22120	18656-18657	.	denotes	.
T22119	18655-18656	-RRB-	denotes	)
T22118	18651-18655	CD	denotes	0.01
T22117	18650-18651	NN	denotes	<
T22116	18649-18650	JJ	denotes	p
T22115	18647-18648	,	denotes	,
T22114	18645-18647	SYM	denotes	**
T22113	18643-18644	:	denotes	;
T22112	18639-18643	CD	denotes	0.05
T22111	18638-18639	RB	denotes	<
T22110	18637-18638	VB	denotes	p
T22109	18635-18636	,	denotes	,
T22108	18634-18635	SYM	denotes	*
T22107	18633-18634	-LRB-	denotes	(
T22106	18622-18632	NN	denotes	expression
T22105	18614-18621	NN	denotes	control
T22104	18603-18613	NN	denotes	luciferase
T22103	18595-18602	NNP	denotes	Renilla
T22102	18592-18594	TO	denotes	to
T22101	18583-18591	VBN	denotes	adjusted
T22100	18579-18582	CC	denotes	and
T22099	18569-18578	VB	denotes	duplicate
T22098	18566-18568	IN	denotes	in
T22097	18556-18565	VBN	denotes	performed
T22096	18544-18555	NNS	denotes	experiments
T22095	18532-18543	JJ	denotes	independent
T22094	18526-18531	CD	denotes	three
T22093	18521-18525	IN	denotes	from
T22092	18517-18520	VBP	denotes	are
T22091	18509-18516	NNS	denotes	Results
T22090	18507-18508	.	denotes	.
T22089	18506-18507	-RRB-	denotes	)
T22088	18502-18506	CD	denotes	0.05
T22087	18501-18502	FW	denotes	<
T22086	18500-18501	FW	denotes	p
T22085	18499-18500	-LRB-	denotes	(
T22084	18492-18498	NN	denotes	extent
T22083	18485-18491	JJR	denotes	lesser
T22082	18483-18484	DT	denotes	a
T22081	18480-18482	TO	denotes	to
T22080	18476-18479	CC	denotes	but
T22079	18470-18475	NNS	denotes	cells
T22078	18460-18469	JJ	denotes	untreated
T22077	18456-18459	DT	denotes	the
T22076	18453-18455	IN	denotes	in
T22075	18442-18452	VBN	denotes	suppressed
T22074	18437-18441	RB	denotes	also
T22073	18433-18436	VBD	denotes	was
T22072	18424-18432	NN	denotes	activity
T22071	18420-18423	NNP	denotes	LTR
T22070	18418-18419	.	denotes	.
T22069	18417-18418	-RRB-	denotes	)
T22068	18413-18417	CD	denotes	0.01
T22067	18412-18413	FW	denotes	<
T22066	18411-18412	FW	denotes	p
T22065	18410-18411	-LRB-	denotes	(
T22064	18403-18409	NN	denotes	lysate
T22063	18399-18402	NNP	denotes	MTb
T22062	18394-18398	IN	denotes	with
T22061	18386-18393	VBN	denotes	treated
T22060	18381-18385	VBD	denotes	were
T22059	18375-18380	NNS	denotes	cells
T22058	18370-18374	WRB	denotes	when
T22057	18366-18369	NNP	denotes	LTR
T22056	18356-18365	JJ	denotes	wild-type
T22055	18352-18355	DT	denotes	the
T22054	18349-18351	TO	denotes	to
T22053	18338-18348	NN	denotes	comparison
T22052	18335-18337	IN	denotes	in
T22051	18324-18334	NN	denotes	expression
T22050	18319-18323	NN	denotes	gene
T22049	18310-18318	NN	denotes	reporter
T22048	18299-18309	JJ	denotes	LTR-driven
T22047	18288-18298	VBD	denotes	suppressed
T22046	18274-18287	RB	denotes	significantly
T22045	18267-18273	NN	denotes	region
T22044	18258-18266	NN	denotes	enhancer
T22043	18254-18257	DT	denotes	the
T22042	18251-18253	TO	denotes	to
T22041	18243-18250	JJ	denotes	binding
T22040	18237-18242	NNP	denotes	NFAT5
T22039	18234-18236	IN	denotes	of
T22038	18223-18233	NNP	denotes	Disruption
T22037	18220-18222	NNP	denotes	C.
T22036	18219-18220	CD	denotes	°
T22035	18217-18219	CD	denotes	37
T22034	18214-18216	IN	denotes	at
T22033	18208-18213	NNS	denotes	hours
T22032	18206-18207	CD	denotes	8
T22031	18202-18205	IN	denotes	for
T22030	18195-18201	NN	denotes	lysate
T22029	18191-18194	NNP	denotes	MTb
T22028	18188-18190	NN	denotes	ml
T22027	18187-18188	NN	denotes	/
T22026	18185-18187	NN	denotes	µg
T22025	18182-18184	CD	denotes	10
T22024	18179-18181	TO	denotes	to
T22023	18171-18178	VBN	denotes	exposed
T22022	18168-18170	CC	denotes	or
T22021	18158-18167	JJ	denotes	untreated
T22020	18153-18157	VBN	denotes	left
T22019	18148-18152	VBD	denotes	were
T22018	18142-18147	NNS	denotes	cells
T22017	18138-18141	DT	denotes	the
T22016	18136-18137	,	denotes	,
T22015	18131-18136	NNS	denotes	hours
T22014	18128-18130	CD	denotes	16
T22013	18122-18127	IN	denotes	After
T22012	18120-18121	.	denotes	.
T22011	18119-18120	-RRB-	denotes	)
T22010	18113-18119	NN	denotes	N5-Mut
T22009	18112-18113	-LRB-	denotes	(
T22008	18102-18111	NNS	denotes	mutations
T22007	18097-18101	NN	denotes	site
T22006	18089-18096	JJ	denotes	binding
T22005	18083-18088	NNP	denotes	NFAT5
T22004	18079-18082	DT	denotes	the
T22003	18068-18078	VBG	denotes	containing
T22002	18064-18067	NNP	denotes	LTR
T22001	18055-18063	JJ	denotes	HIV-1Lai
T22000	18052-18054	DT	denotes	an
T21999	18048-18051	CC	denotes	and
T21998	18044-18047	NNP	denotes	LTR
T21997	18035-18043	JJ	denotes	HIV-1Lai
T21996	18025-18034	JJ	denotes	wild-type
T21995	18021-18024	DT	denotes	the
T21994	18018-18020	IN	denotes	of
T21993	18014-18017	CD	denotes	+64
T21992	18011-18013	TO	denotes	to
T21991	18007-18010	CD	denotes	208
T21990	17995-18006	NNS	denotes	nucleotides
T21989	17986-17994	VBG	denotes	encoding
T21988	17978-17985	NNS	denotes	vectors
T21987	17967-17977	NN	denotes	expression
T21986	17956-17966	NN	denotes	luciferase
T21985	17951-17955	IN	denotes	with
T21984	17939-17950	VBN	denotes	transfected
T21983	17934-17938	VBD	denotes	were
T21982	17928-17933	NNS	denotes	cells
T21981	17922-17927	CD	denotes	THP-1
T21980	17920-17921	.	denotes	.
T21979	17911-17920	NN	denotes	treatment
T21978	17904-17910	NN	denotes	lysate
T21977	17900-17903	NNP	denotes	MTb
T21976	17897-17899	TO	denotes	to
T21975	17888-17896	NN	denotes	response
T21974	17885-17887	IN	denotes	in
T21973	17879-17884	NNS	denotes	cells
T21972	17869-17878	JJ	denotes	monocytic
T21971	17866-17868	IN	denotes	in
T21970	17857-17865	NN	denotes	activity
T21969	17852-17856	NN	denotes	gene
T21968	17839-17851	JJ	denotes	LTR-reporter
T21967	17831-17838	VBZ	denotes	reduces
T21966	17817-17830	RB	denotes	significantly
T21965	17812-17816	NN	denotes	site
T21964	17804-17811	JJ	denotes	binding
T21963	17798-17803	NNP	denotes	NFAT5
T21962	17794-17797	DT	denotes	the
T21961	17791-17793	IN	denotes	of
T21960	17780-17790	NN	denotes	disruption
T21959	17771-17779	JJ	denotes	Specific
T21958	17769-17770	-RRB-	denotes	)
T21957	17768-17769	NNP	denotes	B
T21956	17767-17768	-LRB-	denotes	(
T21955	17765-17766	.	denotes	.
T21954	17764-17765	-RRB-	denotes	)
T21953	17756-17764	NNS	denotes	cultures
T21952	17743-17755	JJ	denotes	unstimulated
T21951	17740-17742	TO	denotes	to
T21950	17731-17739	VBN	denotes	compared
T21949	17728-17730	IN	denotes	as
T21948	17723-17727	CD	denotes	0.01
T21947	17722-17723	NN	denotes	<
T21929	17643-17646	VBP	denotes	are
T21928	17635-17642	NNS	denotes	Results
T21927	17633-17634	.	denotes	.
T21926	17625-17633	NNS	denotes	cultures
T21925	17615-17624	JJ	denotes	untreated
T21924	17612-17614	TO	denotes	to
T21923	17601-17611	NN	denotes	comparison
T21922	17598-17600	IN	denotes	in
T21921	17591-17597	VBP	denotes	lysate
T21920	17587-17590	NNP	denotes	MTb
T21919	17582-17586	IN	denotes	with
T21918	17574-17581	VBN	denotes	treated
T21917	17565-17573	NNS	denotes	cultures
T21916	17562-17564	IN	denotes	in
T21915	17552-17561	VBN	denotes	increased
T21914	17538-17551	RB	denotes	significantly
T21913	17534-17537	VBD	denotes	was
T21912	17527-17533	VBN	denotes	tested
T21911	17522-17526	VBZ	denotes	LTRs
T21910	17507-17521	NN	denotes	representative
T21909	17503-17506	DT	denotes	the
T21908	17500-17502	IN	denotes	of
T21907	17496-17499	DT	denotes	all
T21906	17492-17495	IN	denotes	for
T21905	17483-17491	NN	denotes	activity
T21904	17467-17482	JJ	denotes	transcriptional
T21903	17463-17466	NNP	denotes	LTR
T21902	17461-17462	.	denotes	.
T21901	17455-17461	VBP	denotes	lysate
T21900	17451-17454	NNP	denotes	MTb
T21899	17446-17450	IN	denotes	with
T21898	17438-17445	VBN	denotes	treated
T21897	17432-17437	NNS	denotes	cells
T21896	17429-17431	IN	denotes	in
T21895	17421-17428	NN	denotes	subtype
T21894	17416-17420	DT	denotes	each
T21893	17411-17415	IN	denotes	from
T21892	17400-17410	NNS	denotes	activities
T21891	17396-17399	NNP	denotes	LTR
T21890	17393-17395	IN	denotes	of
T21889	17386-17392	NNS	denotes	values
T21888	17381-17385	JJ	denotes	mean
T21887	17371-17380	VBP	denotes	represent
T21886	17366-17370	NNS	denotes	bars
T21885	17361-17365	NN	denotes	grey
T21884	17356-17360	JJ	denotes	dark
T21883	17352-17355	CC	denotes	and
T21882	17350-17351	,	denotes	,
T21881	17345-17350	NNS	denotes	cells
T21880	17330-17344	JJ	denotes	lysate-treated
T21879	17326-17329	NNP	denotes	MTb
T21878	17323-17325	IN	denotes	in
T21877	17312-17322	NNS	denotes	activities
T21876	17308-17311	NNP	denotes	LTR
T21875	17297-17307	JJ	denotes	individual
T21874	17287-17296	VBP	denotes	represent
T21873	17282-17286	NNS	denotes	bars
T21872	17276-17281	NNP	denotes	Black
T21871	17274-17275	.	denotes	.
T21870	17269-17274	NNS	denotes	cells
T21869	17259-17268	JJ	denotes	untreated
T21868	17256-17258	IN	denotes	in
T21867	17248-17255	NN	denotes	subtype
T21866	17243-17247	DT	denotes	each
T21865	17238-17242	IN	denotes	from
T21864	17227-17237	NNS	denotes	activities
T21863	17223-17226	NNP	denotes	LTR
T21862	17220-17222	IN	denotes	of
T21861	17213-17219	NNS	denotes	values
T21860	17208-17212	JJ	denotes	mean
T21859	17198-17207	VBP	denotes	represent
T21858	17193-17197	NNS	denotes	bars
T21857	17188-17192	NN	denotes	grey
T21856	17182-17187	NN	denotes	Light
T21855	17180-17181	.	denotes	.
T21854	17175-17180	NNS	denotes	cells
T21853	17165-17174	JJ	denotes	untreated
T21852	17162-17164	IN	denotes	in
T21851	17151-17161	NNS	denotes	activities
T21850	17147-17150	NNP	denotes	LTR
T21849	17136-17146	JJ	denotes	individual
T21848	17126-17135	VBP	denotes	represent
T21847	17121-17125	NNS	denotes	bars
T21846	17116-17120	JJ	denotes	open
T21845	17114-17115	,	denotes	,
T21844	17105-17114	NN	denotes	histogram
T21843	17101-17104	DT	denotes	the
T21842	17098-17100	IN	denotes	In
T21841	17096-17097	.	denotes	.
T21840	17088-17096	NNS	denotes	cultures
T21839	17084-17087	DT	denotes	the
T21838	17081-17083	IN	denotes	of
T21837	17069-17080	NN	denotes	termination
T21836	17062-17068	IN	denotes	before
T21835	17056-17061	NNS	denotes	hours
T21834	17054-17055	CD	denotes	8
T21833	17050-17053	IN	denotes	for
T21832	17043-17049	NN	denotes	lysate
T21831	17039-17042	NNP	denotes	MTb
T21830	17036-17038	NN	denotes	ml
T21829	17035-17036	NN	denotes	/
T21828	17033-17035	NN	denotes	µg
T21827	17030-17032	CD	denotes	10
T21826	17025-17029	IN	denotes	with
T21825	17017-17024	VBN	denotes	treated
T21824	17014-17016	CC	denotes	or
T21823	17004-17013	JJ	denotes	untreated
T21822	16999-17003	VBD	denotes	left
T21821	16992-16998	RB	denotes	either
T21820	16987-16991	RB	denotes	then
T21819	16982-16986	VBD	denotes	were
T21818	16976-16981	NNS	denotes	Cells
T21817	16974-16975	.	denotes	.
T21816	16969-16974	NNS	denotes	hours
T21815	16966-16968	CD	denotes	16
T21814	16962-16965	IN	denotes	for
T21813	16960-16961	NNP	denotes	C
T21812	16959-16960	CD	denotes	°
T21811	16957-16959	CD	denotes	37
T21810	16954-16956	IN	denotes	at
T21809	16944-16953	VBD	denotes	incubated
T21808	16940-16943	CC	denotes	and
T21807	16938-16939	-RRB-	denotes	)
T21806	16936-16938	NN	denotes	ml
T21805	16935-16936	NN	denotes	/
T21804	16933-16935	NN	denotes	µg
T21803	16928-16932	CD	denotes	0.03
T21802	16927-16928	-LRB-	denotes	(
T21801	16920-16926	NNP	denotes	pRL-TK
T21800	16912-16919	VBD	denotes	plasmid
T21799	16904-16911	NN	denotes	control
T21798	16893-16903	NN	denotes	luciferase
T21797	16885-16892	NNP	denotes	Renilla
T21796	16881-16884	DT	denotes	the
T21795	16876-16880	CC	denotes	plus
T21794	16874-16875	-RRB-	denotes	)
T21793	16872-16874	NN	denotes	ml
T21792	16871-16872	NN	denotes	/
T21791	16869-16871	NN	denotes	µg
T21790	16865-16868	CD	denotes	0.3
T21789	16864-16865	-LRB-	denotes	(
T21788	16856-16863	NN	denotes	plasmid
T21787	16847-16855	NN	denotes	reporter
T21786	16842-16846	DT	denotes	each
T21785	16837-16841	IN	denotes	with
T21784	16825-16836	VBN	denotes	transfected
T21783	16820-16824	VBD	denotes	were
T21782	16818-16819	-RRB-	denotes	)
T21781	16812-16818	CD	denotes	106/ml
T21780	16811-16812	NN	denotes	×
T21779	16808-16811	CD	denotes	0.8
T21778	16807-16808	-LRB-	denotes	(
T21777	16801-16806	NNS	denotes	cells
T21776	16795-16800	CD	denotes	THP-1
T21775	16793-16794	.	denotes	.
T21774	16789-16793	NN	denotes	pGL3
T21773	16781-16788	JJ	denotes	plasmid
T21772	16776-16780	IN	denotes	into
T21771	16769-16775	VBN	denotes	cloned
T21770	16764-16768	VBD	denotes	were
T21769	16755-16763	VBZ	denotes	isolates
T21768	16749-16754	JJ	denotes	viral
T21767	16747-16748	NNP	denotes	E
T21766	16743-16746	CC	denotes	and
T21765	16741-16742	,	denotes	,
T21764	16740-16741	NNP	denotes	C
T21763	16738-16739	,	denotes	,
T21762	16737-16738	NNP	denotes	B
T21761	16729-16736	NN	denotes	subtype
T21760	16714-16728	NN	denotes	representative
T21759	16709-16713	IN	denotes	from
T21758	16707-16708	-RRB-	denotes	)
T21757	16703-16707	NN	denotes	site
T21756	16697-16702	VB	denotes	start
T21755	16683-16696	NN	denotes	transcription
T21754	16679-16682	DT	denotes	the
T21753	16676-16678	TO	denotes	to
T21752	16667-16675	JJ	denotes	relative
T21751	16664-16666	NN	denotes	nt
T21750	16660-16663	CD	denotes	+64
T21749	16657-16659	TO	denotes	to
T21748	16653-16656	CD	denotes	208
T21747	16652-16653	FW	denotes	−
T21746	16651-16652	-LRB-	denotes	(
T21745	16646-16650	NNP	denotes	LTRs
T21744	16640-16645	NNP	denotes	HIV-1
T21743	16637-16639	NNP	denotes	E.
T21742	16633-16636	CC	denotes	and
T21741	16631-16632	,	denotes	,
T21740	16630-16631	NNP	denotes	C
T22191	19028-19038	JJ	denotes	anti-NFAT5
T22190	19023-19027	IN	denotes	with
T22189	19018-19022	NN	denotes	blot
T22188	19010-19017	JJ	denotes	western
T22187	19007-19009	IN	denotes	by
T22186	18998-19006	VBN	denotes	analyzed
T22185	18994-18997	CC	denotes	and
T22184	18984-18993	VBN	denotes	collected
T22183	18979-18983	VBD	denotes	were
T22182	18970-18978	NNS	denotes	extracts
T22181	18965-18969	NN	denotes	cell
T22180	18959-18964	NNP	denotes	Whole
T22179	18956-18958	NNP	denotes	C.
T22178	18955-18956	CD	denotes	°
T22177	18953-18955	CD	denotes	37
T22176	18950-18952	IN	denotes	at
T22175	18944-18949	NNS	denotes	hours
T22174	18941-18943	CD	denotes	24
T22173	18938-18940	CC	denotes	or
T22172	18936-18937	CD	denotes	8
T22171	18932-18935	IN	denotes	for
T22170	18925-18931	NN	denotes	lysate
T22169	18921-18924	NNP	denotes	MTb
T22168	18918-18920	NN	denotes	ml
T22167	18917-18918	NN	denotes	/
T22166	18915-18917	NN	denotes	µg
T22165	18912-18914	CD	denotes	10
T22164	18909-18911	TO	denotes	to
T22163	18901-18908	VBN	denotes	exposed
T21739	16628-16629	,	denotes	,
T21738	16627-16628	NNP	denotes	B
T21737	16618-16626	NNS	denotes	subtypes
T21736	16612-16617	JJ	denotes	HIV-1
T21735	16607-16611	IN	denotes	from
T21734	16599-16606	VBN	denotes	derived
T21733	16594-16598	NNS	denotes	LTRs
T21732	16591-16593	IN	denotes	of
T21731	16582-16590	NN	denotes	activity
T21730	16572-16581	VBZ	denotes	increases
T21729	16560-16571	NN	denotes	stimulation
T21728	16556-16559	NNP	denotes	MTb
T21727	16554-16555	-RRB-	denotes	)
T21726	16553-16554	NNP	denotes	A
T21725	16552-16553	-LRB-	denotes	(
T21724	16550-16551	.	denotes	.
T21723	16537-16550	NN	denotes	transcription
T21722	16531-16536	NN	denotes	HIV-1
T21721	16519-16530	JJ	denotes	MTb-induced
T21720	16515-16518	IN	denotes	for
T21719	16505-16514	JJ	denotes	important
T21718	16502-16504	VBZ	denotes	is
T21717	16498-16501	NNP	denotes	LTR
T21716	16494-16497	DT	denotes	the
T21715	16489-16493	IN	denotes	with
T21714	16477-16488	NN	denotes	interaction
T21946	17721-17722	JJ	denotes	p
T21945	17719-17720	,	denotes	,
T21944	17717-17719	SYM	denotes	**
T21943	17715-17716	:	denotes	;
T21942	17711-17715	CD	denotes	0.05
T21941	17710-17711	RB	denotes	<
T21940	17709-17710	VB	denotes	p
T21939	17707-17708	,	denotes	,
T21938	17706-17707	SYM	denotes	*
T21937	17705-17706	-LRB-	denotes	(
T21936	17695-17704	VB	denotes	duplicate
T21935	17692-17694	IN	denotes	in
T21934	17682-17691	VBN	denotes	performed
T21933	17670-17681	NNS	denotes	experiments
T21932	17658-17669	JJ	denotes	independent
T21931	17652-17657	CD	denotes	three
T21930	17647-17651	IN	denotes	from
T21713	16471-16476	CD	denotes	NFAT5
T7139	15933-15941	NN	denotes	response
T7138	15930-15932	IN	denotes	in
T7137	15922-15929	VBN	denotes	induced
T7136	15916-15921	DT	denotes	those
T7135	15907-15915	VBP	denotes	resemble
T7134	15902-15906	WDT	denotes	that
T7133	15892-15901	NNS	denotes	monocytes
T7132	15889-15891	IN	denotes	in
T7131	15879-15888	NNS	denotes	responses
T7130	15866-15878	JJ	denotes	inflammatory
T7129	15858-15865	VBZ	denotes	induces
T7128	15851-15857	VBP	denotes	lysate
T7127	15847-15850	NNP	denotes	MTb
T7126	15842-15846	IN	denotes	that
T7125	15837-15841	VBP	denotes	note
T7124	15834-15836	PRP	denotes	We
T7123	15832-15833	.	denotes	.
T7122	15831-15832	-RRB-	denotes	)
T7121	15826-15831	NNP	denotes	H37Rv
T7120	15825-15826	-LRB-	denotes	(
T7119	15821-15824	NNP	denotes	MTb
T7118	15818-15820	IN	denotes	of
T7117	15811-15817	NN	denotes	lysate
T7116	15806-15810	NN	denotes	cell
T7115	15800-15805	JJ	denotes	whole
T7114	15789-15799	JJ	denotes	irradiated
T7113	15786-15788	DT	denotes	an
T7112	15781-15785	IN	denotes	with
T7111	15770-15780	VBN	denotes	stimulated
T7110	15765-15769	VBD	denotes	were
T7109	15759-15764	NNS	denotes	cells
T7108	15757-15758	,	denotes	,
T7107	15749-15757	NNS	denotes	plasmids
T7106	15743-15748	DT	denotes	these
T7105	15738-15742	IN	denotes	with
T7104	15733-15737	NN	denotes	line
T7103	15728-15732	NN	denotes	cell
T7102	15722-15727	CD	denotes	THP-1
T7101	15712-15721	JJ	denotes	monocytic
T7100	15708-15711	DT	denotes	the
T7099	15705-15707	IN	denotes	of
T7098	15692-15704	NN	denotes	transfection
T7097	15686-15691	IN	denotes	After
T7096	15684-15685	.	denotes	.
T7095	15680-15684	NN	denotes	gene
T7094	15671-15679	NN	denotes	reporter
T7093	15660-15670	NN	denotes	luciferase
T7092	15652-15659	JJ	denotes	firefly
T7091	15648-15651	DT	denotes	the
T7090	15645-15647	TO	denotes	to
T7089	15638-15644	VBN	denotes	linked
T7088	15636-15637	-RRB-	denotes	)
T7087	15632-15636	NN	denotes	site
T7086	15626-15631	VB	denotes	start
T7085	15612-15625	NN	denotes	transcription
T7084	15608-15611	DT	denotes	the
T7083	15605-15607	TO	denotes	to
T7082	15596-15604	JJ	denotes	relative
T7081	15593-15595	NN	denotes	nt
T7080	15590-15592	CD	denotes	64
T7079	15588-15589	CD	denotes	+
T7078	15585-15587	TO	denotes	to
T7077	15581-15584	CD	denotes	208
T7076	15580-15581	FW	denotes	−
T7075	15579-15580	-LRB-	denotes	(
T7074	15574-15578	NNP	denotes	LTRs
T7073	15572-15573	NNP	denotes	E
T7072	15568-15571	CC	denotes	and
T7071	15566-15567	,	denotes	,
T7070	15565-15566	NNP	denotes	C
T7069	15563-15564	,	denotes	,
T7068	15562-15563	NNP	denotes	B
T7067	15554-15561	NN	denotes	subtype
T7066	15548-15553	JJ	denotes	viral
T7065	15537-15547	VBG	denotes	containing
T7064	15528-15536	NNS	denotes	plasmids
T7063	15519-15527	NN	denotes	reporter
T7062	15507-15518	VBD	denotes	constructed
T7061	15501-15506	RB	denotes	first
T7060	15498-15500	PRP	denotes	we
T7059	15496-15497	,	denotes	,
T7058	15488-15496	NN	denotes	activity
T7057	15484-15487	NNP	denotes	LTR
T7056	15478-15483	NNP	denotes	HIV-1
T7055	15461-15477	JJ	denotes	subtype-specific
T7054	15458-15460	IN	denotes	on
T7053	15446-15457	NN	denotes	stimulation
T7052	15442-15445	NNP	denotes	MTb
T7051	15439-15441	IN	denotes	of
T7050	15432-15438	NN	denotes	impact
T7049	15421-15431	JJ	denotes	functional
T7048	15417-15420	DT	denotes	the
T7047	15409-15416	VB	denotes	compare
T7046	15406-15408	TO	denotes	To
T7045	15404-15405	NNP	denotes	E
T7044	15400-15403	CC	denotes	and
T7043	15398-15399	,	denotes	,
T7042	15397-15398	NNP	denotes	C
T7041	15395-15396	,	denotes	,
T7040	15394-15395	NNP	denotes	B
T7039	15385-15393	NNS	denotes	subtypes
T7038	15379-15384	JJ	denotes	HIV-1
T7037	15376-15378	IN	denotes	of
T7036	15367-15375	NN	denotes	activity
T7035	15363-15366	NNP	denotes	LTR
T7034	15357-15362	NNP	denotes	HIV-1
T7033	15347-15356	VBZ	denotes	increases
T7032	15343-15346	NNP	denotes	MTb
T6752	15331-15332	.	denotes	.
T6751	15320-15331	JJ	denotes	significant
T6750	15309-15319	VBN	denotes	considered
T6749	15305-15308	VBD	denotes	was
T6748	15300-15304	CD	denotes	0.05
T6747	15299-15300	VBD	denotes	≤
T6746	15298-15299	NN	denotes	p
T6745	15296-15297	.	denotes	.
T6744	15288-15296	NN	denotes	software
T6743	15282-15287	NNP	denotes	Excel
T6742	15272-15281	NNP	denotes	Microsoft
T6741	15269-15271	IN	denotes	of
T6740	15265-15268	NN	denotes	aid
T6739	15261-15264	DT	denotes	the
T6738	15256-15260	IN	denotes	with
T6737	15249-15255	NN	denotes	t-Test
T6736	15241-15248	NN	denotes	Student
T6735	15234-15240	VBN	denotes	paired
T6734	15230-15233	DT	denotes	the
T6733	15224-15229	VBG	denotes	using
T6732	15214-15223	VBN	denotes	performed
T6731	15210-15213	VBD	denotes	was
T6730	15203-15209	NNS	denotes	groups
T6729	15199-15202	CD	denotes	two
T6728	15191-15198	IN	denotes	between
T6727	15180-15190	NN	denotes	Comparison
T6726	15178-15179	.	denotes	.
T6725	15175-15178	NNP	denotes	SEM
T6724	15173-15174	NN	denotes	±
T6723	15168-15172	JJ	denotes	mean
T6722	15165-15167	IN	denotes	as
T6721	15155-15164	VBN	denotes	expressed
T6720	15151-15154	VBP	denotes	are
T6719	15143-15150	NNS	denotes	results
T6718	15141-15142	,	denotes	,
T6717	15131-15141	JJ	denotes	applicable
T6716	15125-15130	WRB	denotes	Where
T6715	15116-15124	NN	denotes	analysis
T6714	15104-15115	NNP	denotes	Statistical
T6666	15101-15102	.	denotes	.
T6665	15095-15101	NNS	denotes	values
T6664	15086-15094	JJ	denotes	relative
T6663	15083-15085	IN	denotes	as
T6662	15073-15082	VBD	denotes	expressed
T6661	15069-15072	CC	denotes	and
T6660	15061-15068	NN	denotes	control
T6659	15052-15060	JJ	denotes	internal
T6658	15049-15051	DT	denotes	an
T6657	15046-15048	IN	denotes	as
T6656	15041-15045	NNP	denotes	mRNA
T6655	15033-15040	JJ	denotes	β-actin
T6654	15027-15032	VBG	denotes	using
T6653	15016-15026	VBN	denotes	normalized
T6652	15011-15015	VBD	denotes	were
T6651	15003-15010	NNS	denotes	results
T6650	14999-15002	DT	denotes	The
T6649	14997-14998	.	denotes	.
T6648	14994-14997	NN	denotes	min
T6647	14992-14993	CD	denotes	1
T6646	14988-14991	IN	denotes	for
T6645	14986-14987	NNP	denotes	C
T6644	14985-14986	NN	denotes	°
T6643	14983-14985	CD	denotes	60
T6642	14979-14982	CC	denotes	and
T6641	14975-14978	NN	denotes	sec
T6640	14972-14974	CD	denotes	15
T6639	14968-14971	IN	denotes	for
T6638	14966-14967	NNP	denotes	C
T6637	14965-14966	CD	denotes	°
T6636	14963-14965	CD	denotes	95
T6635	14960-14962	IN	denotes	of
T6634	14953-14959	NNS	denotes	cycles
T6633	14950-14952	CD	denotes	40
T6632	14947-14949	IN	denotes	by
T6631	14938-14946	VBN	denotes	followed
T6630	14934-14937	NN	denotes	min
T6629	14931-14933	CD	denotes	10
T6628	14927-14930	IN	denotes	for
T6627	14925-14926	NNP	denotes	C
T6626	14924-14925	NN	denotes	°
T6625	14922-14924	CD	denotes	95
T6624	14917-14921	VBD	denotes	were
T6623	14906-14916	NNS	denotes	conditions
T6622	14897-14905	NN	denotes	reaction
T6621	14893-14896	DT	denotes	The
T6620	14891-14892	.	denotes	.
T6619	14890-14891	-RRB-	denotes	)
T6618	14865-14890	NN	denotes	www.appliedbiosystems.com
T6617	14863-14864	,	denotes	,
T6616	14853-14863	NNPS	denotes	Biosystems
T6615	14845-14852	NNP	denotes	Applied
T6614	14844-14845	-LRB-	denotes	(
T6613	14840-14843	NNP	denotes	PCR
T6612	14830-14839	JJ	denotes	real-time
T6611	14818-14829	JJ	denotes	Green-based
T6610	14813-14817	NNP	denotes	SYBR
T6609	14810-14812	IN	denotes	by
T6608	14799-14809	VBN	denotes	determined
T6607	14794-14798	VBD	denotes	were
T6606	14787-14793	NNS	denotes	levels
T6605	14776-14786	NN	denotes	expression
T6604	14771-14775	NNP	denotes	mRNA
T6603	14767-14770	DT	denotes	The
T6602	14763-14766	NNP	denotes	PCR
T6601	14750-14762	JJ	denotes	Quantitative
T7153	15980-15981	NNP	denotes	]
T7152	15978-15980	CD	denotes	37
T7151	15977-15978	NNP	denotes	[
T7150	15975-15976	NNP	denotes	]
T7149	15973-15975	CD	denotes	35
T7148	15972-15973	NNP	denotes	[
T7147	15970-15971	,	denotes	,
T7146	15963-15970	NN	denotes	example
T7145	15959-15962	IN	denotes	for
T7144	15955-15958	VB	denotes	see
T7143	15954-15955	-LRB-	denotes	(
T7142	15950-15953	NNP	denotes	MTb
T7141	15945-15949	VB	denotes	live
T7140	15942-15944	TO	denotes	to
T6485	14747-14748	.	denotes	.
T6484	14746-14747	-RRB-	denotes	)
T6483	14740-14746	NNP	denotes	Pierce
T6482	14739-14740	-LRB-	denotes	(
T6481	14731-14738	NNP	denotes	Reagent
T6480	14714-14730	NNP	denotes	Chemiluminescent
T6479	14709-14713	NNP	denotes	Pico
T6478	14704-14708	NNP	denotes	West
T6477	14692-14703	NNP	denotes	SuperSignal
T6476	14687-14691	IN	denotes	with
T6475	14677-14686	VBN	denotes	developed
T6474	14673-14676	CC	denotes	and
T6473	14668-14672	NNP	denotes	TBST
T6472	14665-14667	IN	denotes	in
T6471	14661-14664	NN	denotes	min
T6470	14659-14660	CD	denotes	5
T6469	14658-14659	NN	denotes	×
T6468	14657-14658	CD	denotes	3
T6467	14650-14656	VBN	denotes	washed
T6466	14644-14649	RB	denotes	again
T6465	14640-14643	VBD	denotes	was
T6464	14635-14639	NN	denotes	blot
T6463	14631-14634	DT	denotes	The
T6462	14629-14630	.	denotes	.
T6461	14628-14629	NN	denotes	h
T6460	14626-14627	CD	denotes	1
T6459	14622-14625	IN	denotes	for
T6458	14610-14621	JJ	denotes	appropriate
T6457	14607-14609	IN	denotes	as
T6456	14605-14606	-RRB-	denotes	)
T6455	14599-14605	NNP	denotes	BioRad
T6454	14598-14599	-LRB-	denotes	(
T6453	14582-14597	JJ	denotes	anti-rabbit-HRP
T6452	14577-14581	VB	denotes	goat
T6451	14574-14576	CC	denotes	or
T6450	14572-14573	-RRB-	denotes	)
T6449	14559-14572	NNP	denotes	Biotechnology
T6448	14554-14558	NNP	denotes	Cruz
T6447	14548-14553	NNP	denotes	Santa
T6446	14547-14548	-LRB-	denotes	(
T6445	14533-14546	JJ	denotes	anti-goat-HRP
T6444	14526-14532	NN	denotes	donkey
T6443	14521-14525	CD	denotes	6000
T6442	14520-14521	CD	denotes	∶
T6441	14519-14520	CD	denotes	1
T6440	14516-14518	IN	denotes	in
T6439	14506-14515	VBN	denotes	incubated
T6438	14502-14505	CC	denotes	and
T6437	14497-14501	NNP	denotes	TBST
T6436	14494-14496	IN	denotes	in
T6435	14490-14493	NN	denotes	min
T6434	14488-14489	CD	denotes	5
T6433	14487-14488	NN	denotes	×
T6432	14486-14487	CD	denotes	3
T6431	14479-14485	VBN	denotes	washed
T6430	14475-14478	VBD	denotes	was
T6429	14470-14474	NN	denotes	blot
T6428	14466-14469	DT	denotes	The
T6427	14464-14465	.	denotes	.
T6426	14453-14464	NN	denotes	temperature
T6425	14448-14452	NN	denotes	room
T6424	14445-14447	IN	denotes	at
T6423	14443-14444	NN	denotes	h
T6422	14441-14442	CD	denotes	2
T6421	14437-14440	IN	denotes	for
T6420	14428-14436	NNP	denotes	BSA/TBST
T6419	14425-14427	IN	denotes	in
T6418	14423-14424	-RRB-	denotes	)
T6417	14410-14423	NNP	denotes	Biotechnology
T6416	14405-14409	NNP	denotes	Cruz
T6415	14399-14404	NNP	denotes	Santa
T6414	14397-14398	:	denotes	;
T6413	14390-14397	JJ	denotes	sc-6217
T6412	14389-14390	-LRB-	denotes	(
T6411	14380-14388	NN	denotes	antibody
T6410	14366-14379	JJ	denotes	anti-Lamin-B1
T6409	14361-14365	NN	denotes	goat
T6408	14358-14360	IN	denotes	of
T6407	14349-14357	NN	denotes	dilution
T6406	14345-14348	CD	denotes	500
T6405	14344-14345	NN	denotes	∶
T6404	14343-14344	CD	denotes	1
T6403	14341-14342	DT	denotes	a
T6402	14337-14340	CC	denotes	and
T6401	14335-14336	-RRB-	denotes	)
T6400	14322-14335	NNP	denotes	Biotechnology
T6399	14317-14321	NNP	denotes	Cruz
T6398	14311-14316	NNP	denotes	Santa
T6397	14310-14311	-LRB-	denotes	(
T6396	14308-14309	-RRB-	denotes	)
T6395	14303-14308	NN	denotes	H-300
T6394	14302-14303	-LRB-	denotes	(
T6393	14293-14301	NN	denotes	antibody
T6392	14282-14292	JJ	denotes	anti-NFAT5
T6391	14275-14281	NN	denotes	rabbit
T6390	14272-14274	IN	denotes	of
T6389	14263-14271	NN	denotes	dilution
T6388	14259-14262	CD	denotes	200
T6387	14258-14259	CD	denotes	∶
T6386	14256-14258	CD	denotes	a1
T6385	14251-14255	IN	denotes	with
T6384	14247-14250	RP	denotes	out
T6383	14239-14246	VBN	denotes	carried
T6382	14235-14238	VBD	denotes	was
T6381	14224-14234	NN	denotes	incubation
T6380	14216-14223	JJ	denotes	Primary
T6379	14214-14215	.	denotes	.
T6378	14213-14214	-RRB-	denotes	)
T6377	14205-14213	NNP	denotes	BSA/TBST
T6376	14204-14205	-LRB-	denotes	(
T6375	14200-14203	CD	denotes	7.6
T6374	14197-14199	NNP	denotes	pH
T6373	14194-14196	IN	denotes	at
T6372	14189-14193	NNP	denotes	NaCl
T6371	14186-14188	NNP	denotes	mM
T6370	14182-14185	CD	denotes	150
T6369	14178-14181	CC	denotes	and
T6368	14173-14177	NNP	denotes	Tris
T6367	14170-14172	NNP	denotes	mM
T6366	14167-14169	CD	denotes	50
T6365	14156-14166	VBG	denotes	containing
T6364	14149-14155	NN	denotes	buffer
T6363	14147-14148	DT	denotes	a
T6362	14144-14146	IN	denotes	in
T6361	14142-14143	-RRB-	denotes	)
T6360	14136-14142	NNP	denotes	BioRad
T6359	14135-14136	-LRB-	denotes	(
T6358	14126-14134	NN	denotes	Tween-20
T6357	14124-14125	NN	denotes	%
T6356	14121-14124	CD	denotes	0.1
T6355	14117-14120	CC	denotes	and
T6354	14115-14116	-RRB-	denotes	)
T6353	14110-14115	NNP	denotes	Sigma
T6352	14109-14110	-LRB-	denotes	(
T6351	14105-14108	NNP	denotes	BSA
T6350	14103-14104	NN	denotes	%
T6349	14102-14103	CD	denotes	4
T6348	14099-14101	IN	denotes	of
T6347	14090-14098	NN	denotes	solution
T6346	14088-14089	DT	denotes	a
T6345	14085-14087	IN	denotes	in
T6344	14083-14084	NNP	denotes	C
T6343	14082-14083	CD	denotes	°
T6342	14080-14082	CD	denotes	37
T6341	14077-14079	IN	denotes	at
T6340	14075-14076	NN	denotes	h
T6339	14073-14074	CD	denotes	1
T6338	14069-14072	IN	denotes	for
T6337	14061-14068	VBN	denotes	blocked
T6336	14056-14060	RB	denotes	then
T6335	14052-14055	VBD	denotes	was
T6334	14047-14051	NN	denotes	blot
T6333	14043-14046	DT	denotes	The
T6332	14041-14042	.	denotes	.
T6331	14040-14041	-RRB-	denotes	)
T6328	14024-14032	NNP	denotes	Membrane
T6327	14015-14023	NNP	denotes	Transfer
T6326	14004-14014	NNP	denotes	Trans-Blot
T6325	13989-14003	JJ	denotes	nitrocellulose
T6324	13987-13988	DT	denotes	a
T6323	13984-13986	TO	denotes	to
T6322	13972-13983	VBN	denotes	transferred
T6321	13968-13971	VBD	denotes	was
T6320	13964-13967	NN	denotes	gel
T6319	13960-13963	DT	denotes	The
T6318	13958-13959	.	denotes	.
T6317	13950-13958	NNP	denotes	SDS-PAGE
T6316	13947-13949	IN	denotes	by
T6315	13937-13946	VBN	denotes	separated
T6314	13932-13936	VBD	denotes	were
T6313	13923-13931	NNS	denotes	proteins
T6312	13919-13922	CC	denotes	and
T6311	13901-13918	JJ	denotes	2-mercaptoethanol
T6310	13897-13900	NN	denotes	v/v
T6309	13895-13896	NN	denotes	%
T6308	13894-13895	CD	denotes	5
T6307	13889-13893	IN	denotes	with
T6306	13882-13888	NN	denotes	buffer
T6305	13875-13881	NN	denotes	sample
T6304	13867-13874	NNP	denotes	Laemmli
T6303	13865-13866	CD	denotes	×
T6302	13864-13865	CD	denotes	1
T6301	13861-13863	IN	denotes	in
T6300	13857-13860	NN	denotes	min
T6299	13855-13856	CD	denotes	5
T6298	13851-13854	IN	denotes	for
T6297	13844-13850	VBN	denotes	boiled
T6296	13839-13843	VBD	denotes	were
T6295	13830-13838	NNS	denotes	Extracts
T6294	13828-13829	.	denotes	.
T6293	13822-13828	NN	denotes	buffer
T6292	13819-13821	IN	denotes	of
T6291	13816-13818	NN	denotes	ml
T6290	13813-13815	CD	denotes	25
T6289	13809-13812	IN	denotes	per
T6288	13807-13808	-RRB-	denotes	)
T6287	13802-13807	NNP	denotes	Roche
T6286	13801-13802	-LRB-	denotes	(
T6285	13792-13800	NNP	denotes	Cocktail
T6284	13782-13791	NNP	denotes	Inhibitor
T6283	13773-13781	NNP	denotes	Protease
T6282	13763-13772	JJ	denotes	EDTA-free
T6281	13754-13762	JJ	denotes	Complete
T6280	13751-13753	IN	denotes	of
T6279	13744-13750	NN	denotes	tablet
T6278	13742-13743	CD	denotes	1
T6277	13738-13741	CC	denotes	and
T6276	13736-13737	,	denotes	,
T6275	13728-13736	NN	denotes	glycerol
T6274	13726-13727	NN	denotes	%
T6273	13724-13726	CD	denotes	10
T6272	13722-13723	,	denotes	,
T6271	13716-13722	NNP	denotes	Triton
T6270	13714-13715	NN	denotes	%
T6269	13713-13714	CD	denotes	1
T6268	13711-13712	,	denotes	,
T6267	13708-13711	CD	denotes	7.5
T6266	13705-13707	NNP	denotes	pH
T6265	13703-13704	,	denotes	,
T6264	13700-13703	NNP	denotes	HCl
T6263	13695-13699	NNP	denotes	Tris
T6262	13692-13694	NNP	denotes	mM
T6261	13689-13691	CD	denotes	50
T6260	13687-13688	,	denotes	,
T6259	13683-13687	NNP	denotes	NaCl
T6258	13680-13682	NNP	denotes	mM
T6257	13676-13679	CD	denotes	150
T6256	13665-13675	VBG	denotes	containing
T6255	13658-13664	NN	denotes	buffer
T6254	13652-13657	NN	denotes	lysis
T6253	13647-13651	IN	denotes	with
T6252	13637-13646	VBN	denotes	collected
T6251	13632-13636	VBD	denotes	were
T6250	13623-13631	NNS	denotes	extracts
T5970	13596-13597	.	denotes	.
T5969	13593-13596	NNP	denotes	MDM
T5968	13585-13592	JJ	denotes	primary
T5967	13582-13584	IN	denotes	of
T5966	13572-13581	NN	denotes	infection
T5965	13569-13571	IN	denotes	of
T5964	13562-13568	NN	denotes	course
T5963	13558-13561	DT	denotes	the
T5962	13553-13557	IN	denotes	over
T5961	13543-13552	VBD	denotes	increased
T5960	13536-13542	NNS	denotes	levels
T5959	13523-13535	NN	denotes	mycobacteria
T5958	13518-13522	IN	denotes	that
T5957	13507-13517	VBG	denotes	confirming
T5956	13505-13506	,	denotes	,
T5955	13493-13505	RB	denotes	respectively
T5954	13491-13492	,	denotes	,
T5953	13488-13491	CD	denotes	104
T5952	13487-13488	CD	denotes	×
T5951	13486-13487	CD	denotes	5
T5950	13482-13485	CC	denotes	and
T5949	13478-13481	CD	denotes	103
T5948	13477-13478	NN	denotes	×
T5947	13476-13477	CD	denotes	6
T5946	13471-13475	VBD	denotes	were
T5945	13464-13470	NNS	denotes	counts
T5944	13460-13463	NNP	denotes	CFU
T5943	13452-13459	JJ	denotes	average
T5942	13448-13451	DT	denotes	the
T5941	13446-13447	CD	denotes	7
T5940	13442-13445	CC	denotes	and
T5939	13440-13441	CD	denotes	4
T5938	13435-13439	NNS	denotes	days
T5937	13432-13434	IN	denotes	on
T5936	13428-13431	CC	denotes	and
T5935	13418-13427	VBN	denotes	performed
T5934	13414-13417	VBD	denotes	was
T5933	13405-13413	NN	denotes	analysis
T5932	13403-13404	-RRB-	denotes	)
T5931	13400-13403	NNP	denotes	CFU
T5930	13399-13400	-LRB-	denotes	(
T5929	13394-13398	NN	denotes	unit
T5928	13379-13393	JJ	denotes	Colony-forming
T5927	13377-13378	.	denotes	.
T5926	13370-13377	NNS	denotes	bacilli
T5925	13369-13370	NN	denotes	∶
T5924	13364-13369	CD	denotes	THP-1
T5923	13360-13363	CC	denotes	and
T5922	13352-13359	NNS	denotes	bacilli
T5921	13350-13351	NN	denotes	∶
T5920	13347-13350	NNP	denotes	MDM
T5919	13345-13346	CD	denotes	1
T5918	13344-13345	CD	denotes	∶
T5917	13343-13344	CD	denotes	1
T5916	13340-13342	CC	denotes	or
T5915	13332-13339	NNS	denotes	bacilli
T6330	14034-14040	NNP	denotes	BioRad
T6329	14033-14034	-LRB-	denotes	(
T5914	13330-13331	NN	denotes	∶
T5913	13326-13330	NNP	denotes	PBMC
T5912	13324-13325	CD	denotes	1
T5911	13323-13324	NN	denotes	∶
T5910	13321-13323	CD	denotes	10
T5909	13318-13320	IN	denotes	at
T5908	13310-13317	NNS	denotes	studies
T5907	13300-13309	NN	denotes	infection
T5906	13295-13299	NN	denotes	cell
T5905	13291-13294	IN	denotes	for
T5904	13284-13290	NN	denotes	medium
T5903	13279-13283	CD	denotes	1640
T5902	13274-13278	NNP	denotes	RPMI
T5901	13269-13273	IN	denotes	with
T5900	13260-13268	NN	denotes	dilution
T5899	13252-13259	JJ	denotes	further
T5898	13245-13251	IN	denotes	before
T5897	13239-13244	CD	denotes	OD650
T5896	13236-13238	IN	denotes	of
T5895	13224-13235	NN	denotes	measurement
T5894	13221-13223	IN	denotes	by
T5893	13210-13220	VBN	denotes	determined
T5892	13205-13209	VBD	denotes	were
T5891	13197-13204	NNS	denotes	numbers
T5890	13192-13196	NN	denotes	cell
T5889	13182-13191	JJ	denotes	Bacterial
T5860	13042-13048	VBN	denotes	plated
T5859	13037-13041	RB	denotes	then
T5858	13032-13036	VBD	denotes	were
T5857	13023-13031	NN	denotes	Bacteria
T5856	13021-13022	.	denotes	.
T5855	13016-13021	NN	denotes	phase
T5854	13009-13015	NN	denotes	growth
T5853	12997-13008	JJ	denotes	logarithmic
T5852	12993-12996	DT	denotes	the
T5851	12990-12992	IN	denotes	in
T5850	12985-12989	VBD	denotes	were
T5849	12980-12984	PRP	denotes	they
T5848	12975-12979	IN	denotes	that
T5847	12968-12974	VB	denotes	ensure
T5846	12965-12967	TO	denotes	to
T5845	12963-12964	NNP	denotes	C
T5844	12962-12963	CD	denotes	°
T5843	12960-12962	CD	denotes	37
T5842	12957-12959	IN	denotes	at
T5841	12953-12956	CD	denotes	0.4
T5840	12950-12952	IN	denotes	of
T5839	12944-12949	NNP	denotes	OD650
T5838	12941-12943	DT	denotes	an
T5837	12938-12940	TO	denotes	to
T5836	12932-12937	VBN	denotes	grown
T5835	12927-12931	VBD	denotes	were
T5834	12918-12926	NNS	denotes	cultures
T5833	12914-12917	DT	denotes	The
T5832	12912-12913	.	denotes	.
T5831	12911-12912	-RRB-	denotes	)
T5830	12891-12911	NN	denotes	www.sigmaaldrich.com
T5829	12889-12890	,	denotes	,
T5828	12876-12889	NNP	denotes	Sigma-Aldrich
T5827	12875-12876	-LRB-	denotes	(
T5826	12872-12874	CD	denotes	80
T5825	12866-12871	CD	denotes	Tween
T5824	12864-12865	NN	denotes	%
T5823	12860-12864	CD	denotes	0.05
T5822	12856-12859	CC	denotes	and
T5821	12854-12855	-RRB-	denotes	)
T5820	12851-12854	NNP	denotes	ADC
T5819	12850-12851	-LRB-	denotes	(
T5818	12842-12849	NN	denotes	complex
T5817	12833-12841	NN	denotes	dextrose
T5816	12825-12832	NN	denotes	albumin
T5815	12820-12824	IN	denotes	with
T5814	12807-12819	VBN	denotes	supplemented
T5813	12805-12806	-RRB-	denotes	)
T5812	12795-12805	NN	denotes	www.bd.com
T5811	12793-12794	,	denotes	,
T5810	12791-12793	NNP	denotes	BD
T5809	12785-12790	NNP	denotes	Difco
T5808	12784-12785	-LRB-	denotes	(
T5807	12777-12783	NN	denotes	medium
T5806	12773-12776	NNP	denotes	7H9
T5805	12761-12772	NNP	denotes	Middlebrook
T5804	12758-12760	IN	denotes	of
T5803	12755-12757	NN	denotes	ml
T5802	12752-12754	CD	denotes	10
T5801	12747-12751	IN	denotes	into
T5800	12741-12746	NN	denotes	stock
T5799	12732-12740	NNS	denotes	bacteria
T5798	12725-12731	JJ	denotes	frozen
T5797	12722-12724	IN	denotes	of
T5796	12719-12721	NN	denotes	µl
T5795	12715-12718	CD	denotes	100
T5794	12708-12714	VBG	denotes	adding
T5793	12705-12707	IN	denotes	by
T5792	12696-12704	VBN	denotes	prepared
T5791	12692-12695	VBD	denotes	was
T5790	12684-12691	NNP	denotes	CDC1551
T5789	12677-12683	NN	denotes	strain
T5788	12668-12676	JJ	denotes	clinical
T5787	12664-12667	NNP	denotes	MTb
T5786	12660-12663	DT	denotes	The
T5785	12652-12659	NN	denotes	culture
T5784	12648-12651	JJ	denotes	MTb
T5465	12082-12091	NN	denotes	packaging
T5464	12078-12081	DT	denotes	the
T5463	12073-12077	IN	denotes	with
T5462	12061-12072	NN	denotes	combination
T5461	12058-12060	IN	denotes	in
T5460	12049-12057	NNS	denotes	plasmids
T5459	12046-12048	CD	denotes	.1
T5458	12042-12046	NNP	denotes	pLKO
T5457	12027-12041	JJ	denotes	shRNA-encoding
T5456	12023-12026	DT	denotes	the
T5455	12018-12022	IN	denotes	with
T5454	12009-12017	NN	denotes	HEK-293T
T5453	12004-12008	NN	denotes	line
T5452	11999-12003	NN	denotes	cell
T5451	11989-11998	NN	denotes	packaging
T5450	11985-11988	DT	denotes	the
T5449	11972-11984	VBG	denotes	transfecting
T5448	11969-11971	IN	denotes	by
T5447	11959-11968	VBN	denotes	generated
T5446	11954-11958	VBD	denotes	were
T5445	11944-11953	NNS	denotes	sequences
T5444	11938-11943	NNP	denotes	shRNA
T5443	11929-11937	VBG	denotes	encoding
T5442	11916-11928	NNP	denotes	Lentiviruses
T5441	11914-11915	.	denotes	.
T5440	11907-11914	NN	denotes	plasmid
T5439	11904-11906	CD	denotes	.1
T5438	11900-11904	NNP	denotes	pLKO
T5437	11896-11899	DT	denotes	the
T5436	11891-11895	IN	denotes	into
T5435	11884-11890	VBN	denotes	cloned
T5434	11879-11883	VBD	denotes	were
T5433	11875-11878	CC	denotes	and
T5432	11864-11874	NN	denotes	laboratory
T5431	11860-11863	PRP$	denotes	our
T5430	11857-11859	IN	denotes	in
T5429	11848-11856	VBN	denotes	designed
T5428	11843-11847	VBD	denotes	were
T5427	11841-11842	-RRB-	denotes	)
T5426	11831-11841	VBN	denotes	underlined
T5425	11828-11830	VBZ	denotes	is
T5424	11819-11827	NN	denotes	sequence
T5423	11812-11818	NN	denotes	target
T5422	11807-11811	NNP	denotes	mRNA
T5421	11801-11806	CD	denotes	TRAF6
T5420	11800-11801	-LRB-	denotes	(
T5419	11798-11799	NN	denotes	′
T5418	11741-11798	NN	denotes	AATTCAAAAAGGAGAAACCTGTTGTGATTTATGAGACGAATCACAACAGGTTTCT-3
T5417	11740-11741	:	denotes	-
T5416	11739-11740	SYM	denotes	′
T5415	11738-11739	CD	denotes	5
T5414	11731-11737	NN	denotes	primer
T5413	11723-11730	VB	denotes	reverse
T5412	11721-11722	,	denotes	,
T5411	11720-11721	NN	denotes	′
T5410	11663-11720	NN	denotes	CCGGAGAAACCTGTTGTGATTCGTCTCATAAATCACAACAGGTTTCTCCTTTTTG-3
T5409	11662-11663	:	denotes	-
T5408	11661-11662	SYM	denotes	′
T5407	11660-11661	CD	denotes	5
T5406	11653-11659	JJR	denotes	primer
T5405	11645-11652	RB	denotes	forward
T5404	11644-11645	-LRB-	denotes	(
T5403	11638-11643	NNP	denotes	TRAF6
T5402	11632-11637	JJ	denotes	human
T5401	11628-11631	CC	denotes	and
T5400	11626-11627	-RRB-	denotes	)
T5399	11616-11626	VBN	denotes	underlined
T5398	11613-11615	VBZ	denotes	is
T5397	11604-11612	NN	denotes	sequence
T5396	11597-11603	NN	denotes	target
T5395	11592-11596	NNP	denotes	mRNA
T5394	11586-11591	NNP	denotes	IRAK1
T5393	11585-11586	-LRB-	denotes	(
T5392	11580-11584	NNP	denotes	mRNA
T5391	11578-11579	NN	denotes	′
T5390	11521-11578	JJ	denotes	AATTCAAAAAGGAGCAGCTGTCCAGGTTTTATGAGACGAAACCTGGACAGCTGCT-3
T5389	11520-11521	:	denotes	-
T5388	11519-11520	SYM	denotes	′
T5387	11518-11519	CD	denotes	5
T5386	11511-11517	NN	denotes	primer
T5385	11503-11510	VB	denotes	reverse
T5384	11501-11502	,	denotes	,
T5383	11500-11501	NN	denotes	′
T5382	11443-11500	NN	denotes	CCGGAGCAGCTGTCCAGGTTTCGTCTCATAAAACCTGGACAGCTGCTCCTTTTTG-3
T5381	11442-11443	:	denotes	-
T5380	11441-11442	SYM	denotes	′
T5379	11440-11441	CD	denotes	5
T5378	11433-11439	JJR	denotes	primer
T5377	11425-11432	RB	denotes	forward
T5376	11424-11425	-LRB-	denotes	(
T5375	11418-11423	NNP	denotes	IRAK1
T5374	11412-11417	JJ	denotes	human
T5373	11402-11411	VBG	denotes	targeting
T5372	11396-11401	NNP	denotes	shRNA
T5371	11394-11395	.	denotes	.
T5370	11384-11394	NN	denotes	laboratory
T5369	11380-11383	PRP$	denotes	our
T5366	11363-11366	VBD	denotes	was
T5365	11359-11362	CC	denotes	and
T5364	11357-11358	-RRB-	denotes	)
T5363	11335-11357	NN	denotes	www.openbiosystems.com
T5362	11334-11335	-LRB-	denotes	(
T5361	11323-11333	NNPS	denotes	Biosystems
T5360	11318-11322	NNP	denotes	Open
T5359	11313-11317	IN	denotes	from
T5358	11303-11312	VBN	denotes	purchased
T5357	11299-11302	VBD	denotes	was
T5356	11293-11298	NNP	denotes	MyD88
T5355	11287-11292	JJ	denotes	human
T5354	11277-11286	VBG	denotes	targeting
T5353	11271-11276	NNP	denotes	shRNA
T5352	11260-11270	VBG	denotes	expressing
T5351	11257-11259	CD	denotes	.1
T5350	11253-11257	NNP	denotes	pLKO
T5349	11245-11252	NN	denotes	plasmid
T5348	11234-11244	JJ	denotes	lentiviral
T5347	11230-11233	DT	denotes	The
T5346	11224-11229	NNP	denotes	shRNA
T5345	11213-11223	VBG	denotes	expressing
T5344	11207-11212	NNS	denotes	cells
T5343	11201-11206	NN	denotes	THP-1
T5342	11194-11200	JJ	denotes	Stable
T5888	13180-13181	.	denotes	.
T5887	13170-13180	NN	denotes	suspension
T5886	13165-13169	NN	denotes	cell
T5885	13158-13164	JJ	denotes	single
T5884	13156-13157	DT	denotes	a
T5883	13153-13155	IN	denotes	in
T5882	13148-13152	VBD	denotes	were
T5881	13139-13147	NNS	denotes	bacteria
T5880	13135-13138	DT	denotes	the
T5879	13130-13134	IN	denotes	that
T5878	13123-13129	VB	denotes	ensure
T5877	13120-13122	TO	denotes	to
T5876	13113-13119	NN	denotes	filter
T5875	13110-13112	NN	denotes	µm
T5874	13108-13109	CD	denotes	5
T5873	13106-13107	DT	denotes	a
T5872	13098-13105	IN	denotes	through
T5871	13091-13097	VBD	denotes	passed
T5870	13087-13090	CC	denotes	and
T5869	13085-13086	,	denotes	,
T5868	13082-13085	NNP	denotes	PBS
T5867	13079-13081	IN	denotes	in
T5866	13067-13078	VBD	denotes	resuspended
T5865	13065-13066	,	denotes	,
T5864	13062-13065	NNP	denotes	PBS
T5863	13057-13061	IN	denotes	with
T5862	13050-13056	VBN	denotes	washed
T5861	13048-13049	,	denotes	,
T5015	11191-11192	.	denotes	.
T5014	11190-11191	NNP	denotes	]
T5013	11188-11190	CD	denotes	31
T5012	11187-11188	NNP	denotes	[
T5011	11177-11186	VBN	denotes	performed
T5010	11172-11176	VBD	denotes	were
T5009	11160-11171	NNS	denotes	experiments
T5008	11150-11159	NN	denotes	infection
T5007	11143-11149	IN	denotes	before
T5006	11132-11142	NN	denotes	expression
T5005	11126-11131	CD	denotes	NFAT5
T5004	11123-11125	IN	denotes	of
T5003	11118-11122	IN	denotes	down
T5002	11112-11117	VB	denotes	knock
T5001	11102-11111	JJ	denotes	efficient
T5000	11098-11101	IN	denotes	for
T4999	11092-11097	NNS	denotes	times
T4998	11088-11091	CD	denotes	two
T4997	11076-11087	VBN	denotes	transfected
T4996	11071-11075	VBD	denotes	were
T4995	11067-11070	NNP	denotes	MDM
T4994	11065-11066	.	denotes	.
T4993	11059-11065	NN	denotes	medium
T4992	11053-11058	JJ	denotes	fresh
T4991	11050-11052	IN	denotes	in
T4990	11040-11049	VBN	denotes	incubated
T4989	11036-11039	CC	denotes	and
T4988	11029-11035	VBN	denotes	washed
T4987	11024-11028	VBD	denotes	were
T4986	11018-11023	NNS	denotes	cells
T4985	11012-11017	WDT	denotes	which
T4984	11006-11011	IN	denotes	after
T4983	10996-11005	RB	denotes	overnight
T4982	10994-10995	NNP	denotes	C
T4981	10993-10994	CD	denotes	°
T4980	10991-10993	CD	denotes	37
T4979	10988-10990	IN	denotes	at
T4978	10983-10987	VBN	denotes	left
T4977	10978-10982	VBD	denotes	were
T4976	10969-10977	NNS	denotes	cultures
T4975	10965-10968	DT	denotes	The
T4974	10963-10964	.	denotes	.
T4973	10958-10963	NN	denotes	serum
T4972	10955-10957	NNP	denotes	AB
T4971	10949-10954	JJ	denotes	human
T4970	10932-10948	JJ	denotes	heat-inactivated
T4969	10930-10931	NN	denotes	%
T5557	12645-12646	.	denotes	.
T5556	12636-12645	NN	denotes	puromycin
T5555	12633-12635	NN	denotes	ml
T5554	12632-12633	NN	denotes	/
T5553	12630-12632	NN	denotes	µg
T5552	12626-12629	CD	denotes	0.8
T5551	12621-12625	IN	denotes	with
T5550	12611-12620	NN	denotes	treatment
T5549	12608-12610	IN	denotes	by
T5548	12599-12607	VBN	denotes	expanded
T5547	12595-12598	CC	denotes	and
T5546	12586-12594	VBN	denotes	selected
T5545	12581-12585	VBD	denotes	were
T5544	12575-12580	NNS	denotes	cells
T5543	12564-12574	VBN	denotes	transduced
T5542	12551-12563	RB	denotes	Successfully
T5541	12549-12550	.	denotes	.
T5540	12546-12549	NNP	denotes	RPM
T5539	12541-12545	CD	denotes	2000
T5538	12538-12540	IN	denotes	at
T5537	12532-12537	NNS	denotes	hours
T5536	12528-12531	CD	denotes	two
T5535	12524-12527	IN	denotes	for
T5534	12510-12523	NN	denotes	spinoculation
T5533	12506-12509	CC	denotes	and
T5532	12504-12505	-RRB-	denotes	)
T5531	12487-12504	NN	denotes	www.millipore.com
T5530	12485-12486	,	denotes	,
T5529	12476-12485	NNP	denotes	Millipore
T5528	12475-12476	-LRB-	denotes	(
T5527	12465-12474	NN	denotes	polybrene
T5526	12462-12464	NN	denotes	ml
T5525	12461-12462	NN	denotes	/
T5524	12459-12461	NN	denotes	µg
T5523	12457-12458	CD	denotes	8
T5522	12454-12456	IN	denotes	of
T5521	12445-12453	NN	denotes	presence
T5520	12441-12444	DT	denotes	the
T5519	12438-12440	IN	denotes	in
T5518	12432-12437	NNS	denotes	cells
T5517	12416-12431	JJ	denotes	virus-producing
T5516	12412-12415	DT	denotes	the
T5515	12407-12411	IN	denotes	from
T5514	12394-12406	NNS	denotes	supernatants
T5513	12389-12393	IN	denotes	with
T5512	12383-12388	NNS	denotes	cells
T5511	12379-12382	DT	denotes	the
T5510	12369-12378	VBG	denotes	culturing
T5509	12366-12368	IN	denotes	by
T5508	12356-12365	NNS	denotes	particles
T5507	12345-12355	JJ	denotes	lentiviral
T5506	12341-12344	DT	denotes	the
T5505	12336-12340	IN	denotes	with
T5504	12325-12335	VBN	denotes	transduced
T5503	12320-12324	VBD	denotes	were
T5502	12314-12319	NNS	denotes	cells
T5501	12308-12313	NNP	denotes	THP-1
T5500	12305-12307	NNP	denotes	C.
T5499	12304-12305	NNP	denotes	°
T5498	12302-12304	CD	denotes	80
T5497	12301-12302	CD	denotes	−
T5496	12298-12300	IN	denotes	at
T5495	12291-12297	VBD	denotes	stored
T5494	12287-12290	CC	denotes	and
T5493	12285-12286	,	denotes	,
T5492	12271-12285	NN	denotes	centrifugation
T5491	12268-12270	IN	denotes	by
T5490	12258-12267	VBN	denotes	clarified
T5489	12256-12257	,	denotes	,
T5488	12239-12256	JJ	denotes	post-transfection
T5487	12233-12238	NNS	denotes	hours
T5486	12230-12232	CD	denotes	48
T5485	12220-12229	VBN	denotes	collected
T5484	12215-12219	VBD	denotes	were
T5483	12202-12214	NNS	denotes	Supernatants
T5482	12200-12201	.	denotes	.
T5481	12199-12200	-RRB-	denotes	)
T5480	12185-12199	NN	denotes	www.qiagen.com
T5479	12183-12184	,	denotes	,
T5478	12177-12183	NNP	denotes	Qiagen
T5477	12176-12177	-LRB-	denotes	(
T5476	12168-12175	NN	denotes	reagent
T5475	12155-12167	NN	denotes	transfection
T5474	12145-12154	NNP	denotes	Effectene
T5473	12139-12144	VBG	denotes	using
T5472	12132-12138	JJ	denotes	pMD2.G
T5471	12124-12131	VBD	denotes	plasmid
T5470	12115-12123	NN	denotes	envelope
T5469	12111-12114	DT	denotes	the
T5468	12107-12110	CC	denotes	and
T5467	12100-12106	NNP	denotes	psPAX2
T5466	12092-12099	VBD	denotes	plasmid
T4968	10929-10930	CD	denotes	5
T4967	10924-10928	CC	denotes	plus
T4966	10917-10923	NN	denotes	medium
T4965	10902-10916	NNP	denotes	Macrophage-SFM
T4964	10899-10901	IN	denotes	in
T4963	10896-10898	NN	denotes	µl
T4962	10892-10895	CD	denotes	750
T4961	10889-10891	IN	denotes	of
T4960	10882-10888	NN	denotes	volume
T4959	10876-10881	JJ	denotes	final
T4958	10874-10875	DT	denotes	a
T4957	10871-10873	IN	denotes	in
T4956	10869-10870	,	denotes	,
T4955	10857-10869	NN	denotes	manufacturer
T4954	10853-10856	DT	denotes	the
T4953	10850-10852	IN	denotes	by
T4952	10838-10849	VBN	denotes	recommended
T4951	10835-10837	IN	denotes	as
T4950	10826-10834	VBN	denotes	prepared
T4949	10824-10825	,	denotes	,
T4948	10823-10824	-RRB-	denotes	)
T4947	10809-10823	NN	denotes	www.ambion.com
T4946	10807-10808	,	denotes	,
T4945	10803-10807	NNP	denotes	Inc.
T4944	10796-10802	NNP	denotes	Ambion
T4943	10795-10796	-LRB-	denotes	(
T4888	10518-10521	CD	denotes	335
T4887	10515-10517	JJ	denotes	nt
T4886	10514-10515	-LRB-	denotes	(
T4885	10512-10513	NN	denotes	′
T4884	10491-10512	NN	denotes	CAACATGCCTGGAATTCAA-3
T4883	10490-10491	:	denotes	-
T4882	10489-10490	SYM	denotes	′
T4881	10488-10489	CD	denotes	5
T4880	10486-10487	:	denotes	:
T4879	10476-10486	NN	denotes	transcript
T4878	10470-10475	NNP	denotes	NFAT5
T4877	10466-10469	DT	denotes	the
T4876	10463-10465	TO	denotes	to
T4875	10456-10462	JJ	denotes	unique
T4874	10447-10455	NN	denotes	sequence
T4873	10445-10446	DT	denotes	a
T4872	10438-10444	VB	denotes	target
T4871	10435-10437	TO	denotes	to
T4870	10433-10434	-RRB-	denotes	)
T4869	10419-10433	NN	denotes	www.ambion.com
T4868	10417-10418	,	denotes	,
T4867	10413-10417	NNP	denotes	Inc.
T4866	10406-10412	NNP	denotes	Ambion
T4865	10405-10406	-LRB-	denotes	(
T4864	10393-10404	VBN	denotes	constructed
T4863	10389-10392	VBD	denotes	was
T4862	10383-10388	NN	denotes	siRNA
T4861	10380-10382	DT	denotes	An
T4860	10376-10379	NNP	denotes	MDM
T4859	10373-10375	IN	denotes	of
T4858	10360-10372	NN	denotes	transfection
T4857	10354-10359	NNP	denotes	siRNA
T4531	9852-9854	IN	denotes	of
T4530	9848-9851	CD	denotes	717
T4529	9844-9847	CD	denotes	103
T4528	9838-9843	NNS	denotes	acids
T4527	9832-9837	JJ	denotes	amino
T4526	9826-9831	CD	denotes	gp160
T4525	9824-9825	-RRB-	denotes	)
T4524	9821-9824	FW	denotes	env
T4523	9820-9821	-LRB-	denotes	(
T4522	9811-9819	NN	denotes	envelope
T4521	9807-9810	DT	denotes	the
T4520	9797-9806	VBG	denotes	replacing
T4519	9794-9796	IN	denotes	by
T4518	9782-9793	VBN	denotes	constructed
T4517	9778-9781	VBD	denotes	was
T4516	9772-9777	NN	denotes	clone
T4515	9762-9771	JJ	denotes	molecular
T4514	9751-9761	JJ	denotes	infectious
T4513	9743-9750	NNP	denotes	Bal-Env
T4512	9742-9743	NN	denotes	/
T4511	9734-9742	JJ	denotes	HIV-1Lai
T4510	9730-9733	DT	denotes	The
T4509	9728-9729	.	denotes	.
T4508	9721-9728	NNP	denotes	Program
T4507	9711-9720	NNP	denotes	Reference
T4506	9707-9710	CC	denotes	and
T4505	9699-9706	NNP	denotes	Reagent
T4504	9694-9698	NNP	denotes	AIDS
T4503	9690-9693	NNP	denotes	NIH
T4502	9686-9689	DT	denotes	the
T4501	9681-9685	IN	denotes	from
T4500	9672-9680	VBN	denotes	obtained
T4499	9668-9671	VBD	denotes	was
T4498	9659-9667	NN	denotes	HIV-1Lai
T4497	9656-9658	IN	denotes	of
T4496	9650-9655	NN	denotes	clone
T4495	9640-9649	JJ	denotes	molecular
T4494	9629-9639	JJ	denotes	infectious
T4493	9617-9628	JJ	denotes	full-length
T4492	9613-9616	DT	denotes	the
T4491	9604-9612	VBG	denotes	encoding
T4490	9596-9603	JJ	denotes	plasmid
T4489	9592-9595	DT	denotes	The
T4488	9585-9591	NNS	denotes	clones
T4487	9575-9584	JJ	denotes	molecular
T4486	9564-9574	JJ	denotes	infectious
T4485	9558-9563	CD	denotes	HIV-1
T4942	10787-10794	NN	denotes	reagent
T4941	10774-10786	NN	denotes	transfection
T4940	10768-10773	NNP	denotes	NeoFX
T4939	10761-10767	NNP	denotes	siPORT
T4938	10758-10760	IN	denotes	in
T4937	10752-10757	NN	denotes	siRNA
T4936	10742-10751	VBN	denotes	indicated
T4935	10738-10741	DT	denotes	the
T4934	10735-10737	IN	denotes	of
T4933	10732-10734	NN	denotes	µM
T4932	10730-10731	CD	denotes	1
T4931	10724-10729	VBG	denotes	using
T4930	10717-10723	NNS	denotes	plates
T4929	10710-10716	JJ	denotes	6-well
T4928	10707-10709	IN	denotes	in
T4927	10695-10706	VBN	denotes	transfected
T4926	10690-10694	VBD	denotes	were
T4925	10686-10689	NNP	denotes	MDM
T4924	10684-10685	.	denotes	.
T4923	10683-10684	NN	denotes	′
T4922	10660-10683	NN	denotes	GGCTACGTCCAGGAGCGCACC-3
T4921	10658-10659	:	denotes	-
T4920	10657-10658	SYM	denotes	′
T4919	10656-10657	CD	denotes	5
T4918	10654-10655	,	denotes	,
T4917	10653-10654	-RRB-	denotes	)
T4916	10650-10653	NNP	denotes	GFP
T4915	10649-10650	-LRB-	denotes	(
T4914	10641-10648	NN	denotes	protein
T4913	10629-10640	JJ	denotes	fluorescent
T4912	10623-10628	JJ	denotes	green
T4911	10619-10622	DT	denotes	the
T4910	10609-10618	VBG	denotes	targeting
T4909	10603-10608	JJ	denotes	siRNA
T4908	10600-10602	DT	denotes	an
T4907	10595-10599	VBD	denotes	used
T4906	10592-10594	PRP	denotes	we
T4905	10590-10591	,	denotes	,
T4904	10583-10590	NNS	denotes	effects
T4903	10577-10582	NNP	denotes	siRNA
T4902	10564-10576	JJ	denotes	non-specific
T4901	10560-10563	IN	denotes	for
T4900	10552-10559	NN	denotes	control
T4899	10550-10551	DT	denotes	a
T4898	10548-10549	,	denotes	,
T4897	10539-10548	VBN	denotes	described
T4896	10536-10538	IN	denotes	As
T4895	10534-10535	.	denotes	.
T4894	10533-10534	NNP	denotes	]
T4893	10531-10533	CD	denotes	31
T4892	10530-10531	NNP	denotes	[
T4891	10528-10529	-RRB-	denotes	)
T4890	10525-10528	CD	denotes	353
T4889	10522-10524	TO	denotes	to
T4216	9178-9190	VBG	denotes	footprinting
T4215	9176-9177	PRP	denotes	I
T4214	9170-9175	NNP	denotes	DNase
T4213	9157-9169	JJ	denotes	Quantitative
T4040	9154-9155	.	denotes	.
T4039	9153-9154	-RRB-	denotes	)
T4038	9138-9153	NN	denotes	www.promega.com
T4037	9136-9137	:	denotes	;
T4036	9129-9136	JJ	denotes	Promega
T4035	9128-9129	-LRB-	denotes	(
T4034	9119-9127	NN	denotes	protocol
T4033	9116-9118	POS	denotes	's
T4032	9104-9116	NN	denotes	manufacturer
T4031	9100-9103	DT	denotes	the
T4030	9097-9099	TO	denotes	to
T4029	9087-9096	VBG	denotes	according
T4028	9081-9086	NN	denotes	assay
T4027	9072-9080	NN	denotes	reporter
T4611	10351-10352	.	denotes	.
T4610	10344-10351	NNS	denotes	methods
T4609	10332-10343	NN	denotes	mutagenesis
T4608	10322-10331	JJ	denotes	PCR-based
T4607	10313-10321	JJ	denotes	standard
T4606	10307-10312	VBG	denotes	using
T4605	10297-10306	NNS	denotes	mutations
T4604	10291-10296	NN	denotes	point
T4603	10279-10290	VBG	denotes	introducing
T4602	10276-10278	IN	denotes	by
T4601	10264-10275	VBN	denotes	constructed
T4600	10259-10263	VBD	denotes	were
T4599	10251-10258	NNS	denotes	viruses
T4598	10244-10250	JJ	denotes	mutant
T4597	10232-10243	NNP	denotes	HIV-198IN22
T4596	10228-10231	CC	denotes	and
T4595	10220-10227	NNP	denotes	Bal-Env
T4594	10219-10220	NN	denotes	/
T4593	10211-10219	JJ	denotes	HIV-1Lai
T4592	10209-10210	.	denotes	.
T4579	10129-10132	NNP	denotes	DNA
T4578	10123-10128	VBG	denotes	using
T4577	10111-10122	VBN	denotes	constructed
T4576	10107-10110	VBD	denotes	was
T4575	10095-10106	NN	denotes	HIV-198IN22
T4574	10092-10094	IN	denotes	of
T4573	10086-10091	NN	denotes	clone
T4572	10076-10085	JJ	denotes	molecular
T4571	10065-10075	JJ	denotes	infectious
T4570	10061-10064	DT	denotes	The
T4569	10059-10060	.	denotes	.
T4568	10051-10059	NN	denotes	receptor
T4567	10041-10050	JJ	denotes	secondary
T4566	10039-10040	DT	denotes	a
T4565	10036-10038	IN	denotes	as
T4564	10031-10035	CD	denotes	CCR5
T4563	10026-10030	VBZ	denotes	uses
T4562	10020-10025	NN	denotes	virus
T4561	10011-10019	JJ	denotes	chimeric
T4560	10003-10010	NNP	denotes	Bal-Env
T4559	10002-10003	NN	denotes	/
T4558	9994-10002	JJ	denotes	HIV-1Lai
T4557	9990-9993	DT	denotes	The
T4556	9988-9989	.	denotes	.
T4555	9987-9988	-RRB-	denotes	)
T4554	9983-9987	NNP	denotes	CCR5
T4553	9974-9982	VBZ	denotes	utilizes
T4552	9969-9973	WDT	denotes	that
T4551	9961-9968	NN	denotes	subtype
T4550	9959-9960	NNP	denotes	B
T4549	9958-9959	-LRB-	denotes	(
T4548	9949-9957	JJ	denotes	HIV-1Bal
T4547	9946-9948	IN	denotes	of
T4546	9939-9945	NN	denotes	region
T4545	9925-9938	JJ	denotes	corresponding
T4544	9921-9924	DT	denotes	the
T4543	9916-9920	IN	denotes	with
T4542	9910-9915	NN	denotes	clone
T4541	9900-9909	JJ	denotes	molecular
T4540	9898-9899	-RRB-	denotes	)
T4539	9893-9898	NNP	denotes	CXCR4
T4538	9884-9892	VBZ	denotes	utilizes
T4537	9879-9883	WDT	denotes	that
T4536	9871-9878	NN	denotes	subtype
T4535	9869-9870	NNP	denotes	B
T4534	9868-9869	-LRB-	denotes	(
T4533	9859-9867	NNP	denotes	HIV-1Lai
T4532	9855-9858	DT	denotes	the
T4026	9056-9071	JJ	denotes	dual-luciferase
T4025	9053-9055	IN	denotes	by
T4024	9041-9052	VBN	denotes	quantitated
T4023	9037-9040	VBD	denotes	was
T4022	9026-9036	NN	denotes	expression
T4021	9021-9025	NN	denotes	gene
T4020	9012-9020	NN	denotes	Reporter
T4019	9010-9011	.	denotes	.
T4018	9005-9010	NNS	denotes	hours
T4017	9003-9004	CD	denotes	8
T4016	8999-9002	IN	denotes	for
T4015	8986-8998	JJ	denotes	unstimulated
T4014	8981-8985	VBD	denotes	left
T4013	8978-8980	CC	denotes	or
T4012	8971-8977	VBP	denotes	lysate
T4011	8963-8970	NNP	denotes	CDC1551
T4010	8959-8962	NNP	denotes	MTb
T4009	8956-8958	NN	denotes	ml
T4008	8955-8956	NN	denotes	/
T4007	8953-8955	NN	denotes	µg
T4006	8950-8952	CD	denotes	10
T4005	8945-8949	IN	denotes	with
T4004	8934-8944	VBN	denotes	stimulated
T4003	8929-8933	VBD	denotes	were
T4002	8924-8928	PRP	denotes	they
T4001	8918-8923	WDT	denotes	which
T4237	9286-9290	NNP	denotes	BL21
T4236	9281-9285	NNS	denotes	coli
T4235	9278-9280	NNP	denotes	E.
T4234	9275-9277	IN	denotes	in
T4233	9265-9274	VBN	denotes	expressed
T4232	9261-9264	VBD	denotes	was
T4231	9257-9260	NN	denotes	tag
T4230	9253-9256	PRP$	denotes	His
T4229	9251-9252	NN	denotes	×
T4228	9250-9251	CD	denotes	6
T4227	9239-9249	JJ	denotes	N-terminal
T4226	9236-9238	DT	denotes	an
T4225	9231-9235	IN	denotes	with
T4224	9229-9230	-RRB-	denotes	)
T4223	9226-9229	CD	denotes	471
T4222	9222-9225	CD	denotes	175
T4221	9216-9221	NNS	denotes	acids
T4220	9210-9215	JJ	denotes	amino
T4219	9209-9210	-LRB-	denotes	(
T4218	9203-9208	NNS	denotes	NFAT5
T4217	9191-9202	JJ	denotes	Recombinant
T3374	7338-7342	NN	denotes	flow
T3373	7335-7337	IN	denotes	by
T3372	7326-7334	VBN	denotes	verified
T3371	7323-7325	IN	denotes	as
T3370	7311-7322	NNS	denotes	macrophages
T3369	7309-7310	NN	denotes	+
T3368	7305-7309	CD	denotes	CD14
T3367	7300-7304	VBD	denotes	were
T3366	7290-7299	NN	denotes	technique
T3365	7285-7289	DT	denotes	this
T3364	7280-7284	IN	denotes	with
T3363	7271-7279	VBN	denotes	obtained
T3362	7265-7270	NNS	denotes	cells
T3361	7256-7264	JJ	denotes	adherent
T3360	7252-7255	DT	denotes	the
T3359	7249-7251	IN	denotes	of
T3358	7247-7248	NN	denotes	%
T3357	7245-7247	CD	denotes	95
T3356	7240-7244	IN	denotes	than
T3355	7235-7239	JJR	denotes	More
T3354	7233-7234	.	denotes	.
T3353	7221-7233	NN	denotes	manipulation
T3352	7214-7220	IN	denotes	before
T3351	7209-7213	NNP	denotes	MCSF
T3350	7201-7208	VBG	denotes	lacking
T3349	7194-7200	NN	denotes	medium
T3348	7188-7193	JJ	denotes	fresh
T3347	7183-7187	IN	denotes	with
T3346	7174-7182	VBN	denotes	replaced
T3345	7170-7173	VBD	denotes	was
T3344	7158-7169	NN	denotes	supernatant
T3343	7152-7157	WDT	denotes	which
T3342	7146-7151	IN	denotes	after
T3341	7144-7145	,	denotes	,
T3340	7140-7144	NNS	denotes	days
T3339	7138-7139	CD	denotes	5
T3338	7134-7137	IN	denotes	for
T3337	7130-7133	CD	denotes	CO2
T3336	7128-7129	NN	denotes	%
T3335	7127-7128	CD	denotes	5
T3334	7123-7126	CC	denotes	and
T3333	7121-7122	NNP	denotes	C
T3332	7120-7121	CD	denotes	°
T3331	7118-7120	CD	denotes	37
T3330	7115-7117	IN	denotes	at
T3329	7105-7114	VBN	denotes	incubated
T3328	7100-7104	VBD	denotes	were
T3327	7091-7099	NNS	denotes	cultures
T3326	7086-7090	NN	denotes	cell
T3325	7082-7085	DT	denotes	The
T3324	7080-7081	.	denotes	.
T3323	7079-7080	-RRB-	denotes	)
T3322	7077-7079	NNP	denotes	FL
T3321	7075-7076	,	denotes	,
T3320	7070-7075	NNP	denotes	Raton
T3319	7065-7069	NNP	denotes	Boca
T3318	7063-7064	,	denotes	,
T3317	7059-7063	NNP	denotes	Nabi
T3316	7058-7059	-LRB-	denotes	(
T3315	7052-7057	NN	denotes	serum
T3314	7049-7051	NNP	denotes	AB
T3313	7043-7048	JJ	denotes	human
T3312	7026-7042	JJ	denotes	heat-inactivated
T3311	7024-7025	NN	denotes	%
T3310	7023-7024	CD	denotes	5
T3309	7019-7022	CC	denotes	and
T3308	7017-7018	-RRB-	denotes	)
T3307	6999-7017	NN	denotes	www.rndsystems.com
T3306	6997-6998	,	denotes	,
T3305	6994-6997	NNP	denotes	R&D
T3304	6993-6994	-LRB-	denotes	(
T3303	6988-6992	NNP	denotes	MCSF
T3302	6982-6987	JJ	denotes	human
T3301	6970-6981	JJ	denotes	recombinant
T3300	6964-6969	NN	denotes	ng/ml
T3299	6961-6963	CD	denotes	15
T3298	6956-6960	IN	denotes	with
T3297	6943-6955	VBN	denotes	supplemented
T3296	6941-6942	-RRB-	denotes	)
T3295	6923-6941	NN	denotes	www.invitrogen.com
T3294	6921-6922	,	denotes	,
T3293	6916-6921	NNP	denotes	Gibco
T3292	6915-6916	-LRB-	denotes	(
T3291	6908-6914	NN	denotes	medium
T3290	6893-6907	NNP	denotes	Macrophage-SFM
T3289	6890-6892	IN	denotes	in
T3288	6883-6889	NNS	denotes	plates
T3287	6876-6882	JJ	denotes	6-well
T3286	6873-6875	IN	denotes	in
T3285	6868-6872	RB	denotes	well
T3284	6864-6867	IN	denotes	per
T3283	6858-6863	NNS	denotes	cells
T3282	6854-6857	CD	denotes	106
T3281	6853-6854	CD	denotes	×
T3280	6852-6853	CD	denotes	1
T3279	6849-6851	IN	denotes	at
T3278	6840-6848	VBN	denotes	cultured
T3277	6835-6839	VBD	denotes	were
T3276	6831-6834	CC	denotes	and
T3275	6829-6830	,	denotes	,
T3274	6817-6829	NN	denotes	manufacturer
T3273	6813-6816	DT	denotes	the
T3272	6810-6812	IN	denotes	by
T3271	6800-6809	VBN	denotes	described
T3270	6797-6799	IN	denotes	as
T3269	6795-6796	-RRB-	denotes	)
T3268	6773-6795	NN	denotes	www.miltenyibiotec.com
T3267	6772-6773	-LRB-	denotes	(
T3266	6765-6771	NNP	denotes	Biotec
T3265	6756-6764	NNP	denotes	Miltenyi
T3264	6751-6755	IN	denotes	from
T3263	6740-6750	NNS	denotes	microbeads
T3262	6735-6739	CD	denotes	CD14
T3261	6730-6734	IN	denotes	with
T3260	6720-6729	NN	denotes	selection
T3259	6711-6719	JJ	denotes	positive
T3258	6708-6710	IN	denotes	by
T3257	6695-6707	NNS	denotes	preparations
T3256	6690-6694	NNP	denotes	PBMC
T3255	6685-6689	IN	denotes	from
T3254	6676-6684	VBN	denotes	isolated
T3253	6671-6675	VBD	denotes	were
T3252	6661-6670	NNS	denotes	monocytes
T3251	6655-6660	JJ	denotes	Human
T3250	6653-6654	.	denotes	.
T3249	6652-6653	-RRB-	denotes	)
T3248	6638-6652	NN	denotes	www.gembio.com
T3247	6636-6637	,	denotes	,
T3246	6624-6636	NNP	denotes	Bio-Products
T3245	6617-6623	NNP	denotes	Gemini
T3244	6616-6617	-LRB-	denotes	(
T3243	6614-6615	-RRB-	denotes	)
T3242	6611-6614	NNP	denotes	FCS
T3241	6610-6611	-LRB-	denotes	(
T3240	6604-6609	NN	denotes	serum
T3239	6599-6603	NN	denotes	calf
T3238	6593-6598	JJ	denotes	fetal
T3237	6576-6592	JJ	denotes	heat-inactivated
T3236	6574-6575	NN	denotes	%
T3235	6572-6574	CD	denotes	10
T3234	6567-6571	IN	denotes	with
T3233	6554-6566	VBN	denotes	supplemented
T3232	6552-6553	-RRB-	denotes	)
T3231	6550-6552	NNP	denotes	MD
T3230	6548-6549	,	denotes	,
T3229	6536-6548	NNP	denotes	Walkersville
T3228	6534-6535	,	denotes	,
T3227	6530-6534	NNP	denotes	Inc.
T3226	6528-6529	,	denotes	,
T3225	6516-6528	NNP	denotes	BioWhittaker
T3224	6515-6516	-LRB-	denotes	(
T3223	6503-6514	NNP	denotes	L-glutamine
T3222	6500-6502	NNP	denotes	mM
T3221	6498-6499	CD	denotes	2
T3220	6493-6497	IN	denotes	with
T3219	6486-6492	NN	denotes	medium
T3218	6481-6485	CD	denotes	1640
T3217	6476-6480	NNP	denotes	RPMI
T3216	6473-6475	IN	denotes	in
T3215	6464-6472	VBN	denotes	cultured
T3214	6459-6463	VBD	denotes	were
T3213	6455-6458	CC	denotes	and
T3212	6440-6454	NN	denotes	centrifugation
T3211	6431-6439	NN	denotes	gradient
T3210	6423-6430	NN	denotes	density
T3209	6421-6422	-RRB-	denotes	)
T3208	6419-6421	NNP	denotes	NJ
T3983	8836-8843	NN	denotes	reagent
T3982	8823-8835	NN	denotes	transfection
T3981	8813-8822	NNP	denotes	Effectene
T3980	8807-8812	VBG	denotes	using
T3979	8800-8806	NN	denotes	vector
T3978	8792-8799	NN	denotes	control
T3977	8790-8791	-RRB-	denotes	)
T3976	8784-8790	JJ	denotes	pRL-TK
T3975	8783-8784	-LRB-	denotes	(
T3974	8772-8782	NN	denotes	luciferase
T3973	8764-8771	NN	denotes	Renilla
T3972	8761-8763	NN	denotes	ml
T3971	8760-8761	NN	denotes	/
T3970	8758-8760	NN	denotes	µg
T3969	8753-8757	CD	denotes	0.03
T3968	8748-8752	IN	denotes	with
T3967	8736-8747	NN	denotes	combination
T3966	8733-8735	IN	denotes	in
T3965	8724-8732	NNS	denotes	plasmids
T3964	8715-8723	NN	denotes	reporter
T3963	8707-8714	VBN	denotes	mutated
T3962	8704-8706	CC	denotes	or
T3961	8702-8703	-RRB-	denotes	)
T3960	8700-8702	NNP	denotes	WT
T3959	8699-8700	-LRB-	denotes	(
T3958	8689-8698	JJ	denotes	wild-type
T3957	8685-8688	NNP	denotes	LTR
T3956	8682-8684	NN	denotes	ml
T3955	8681-8682	NN	denotes	/
T3954	8679-8681	NN	denotes	µg
T3953	8675-8678	CD	denotes	0.3
T3952	8670-8674	IN	denotes	with
T3951	8658-8669	VBN	denotes	transfected
T3950	8653-8657	VBD	denotes	were
T3949	8651-8652	-RRB-	denotes	)
T3948	8645-8651	CD	denotes	106/ml
T3947	8644-8645	NN	denotes	×
T3946	8641-8644	CD	denotes	0.8
T3945	8640-8641	-LRB-	denotes	(
T3944	8634-8639	NNS	denotes	cells
T3943	8628-8633	CD	denotes	THP-1
T3942	8626-8627	.	denotes	.
T3941	8625-8626	NNP	denotes	]
T3940	8623-8625	CD	denotes	34
T3939	8622-8623	NNP	denotes	[
T3938	8614-8621	NNS	denotes	methods
T3937	8602-8613	NN	denotes	mutagenesis
T3936	8592-8601	JJ	denotes	PCR-based
T3935	8583-8591	JJ	denotes	standard
T3934	8580-8582	IN	denotes	by
T3933	8572-8579	VBN	denotes	created
T3932	8568-8571	VBD	denotes	was
T3931	8560-8567	NN	denotes	plasmid
T3930	8551-8559	NN	denotes	reporter
T3929	8549-8550	-RRB-	denotes	)
T3928	8543-8549	JJ	denotes	N5-Mut
T3927	8542-8543	-LRB-	denotes	(
T3926	8530-8541	JJ	denotes	site-mutant
T3925	8522-8529	JJ	denotes	binding
T3924	8516-8521	CD	denotes	NFAT5
T3923	8507-8515	JJ	denotes	HIV-1Lai
T3922	8503-8506	DT	denotes	The
T3921	8501-8502	.	denotes	.
T3920	8500-8501	-RRB-	denotes	)
T3919	8477-8500	NNP	denotes	www.ebi.ac.uk/clustalw/
T3918	8476-8477	-LRB-	denotes	(
T3917	8474-8475	NNP	denotes	W
T3916	8466-8473	NNP	denotes	CLUSTAL
T3915	8461-8465	IN	denotes	with
T3914	8452-8460	VBN	denotes	analyzed
T3913	8448-8451	CC	denotes	and
T3912	8440-8447	VBN	denotes	aligned
T3911	8435-8439	VBD	denotes	were
T3910	8425-8434	NNS	denotes	Sequences
T3909	8423-8424	.	denotes	.
T3908	8418-8423	NNS	denotes	sites
T3907	8411-8417	NN	denotes	enzyme
T3906	8399-8410	NN	denotes	restriction
T3905	8395-8398	NNP	denotes	III
T3904	8390-8394	NNP	denotes	Hind
T3903	8386-8389	CC	denotes	and
T3902	8384-8385	PRP	denotes	I
T3901	8380-8383	NNP	denotes	Xho
T3900	8374-8379	VBG	denotes	using
T3899	8372-8373	-RRB-	denotes	)
T3898	8357-8372	NN	denotes	www.promega.com
T3897	8355-8356	,	denotes	,
T3896	8344-8355	NNP	denotes	BioSciences
T3895	8336-8343	NNP	denotes	Promega
T3894	8335-8336	-LRB-	denotes	(
T3893	8330-8334	NNP	denotes	pGL3
T3892	8323-8329	NN	denotes	vector
T3891	8314-8322	NN	denotes	reporter
T3890	8310-8313	DT	denotes	the
T3889	8305-8309	IN	denotes	into
T3888	8303-8304	-RRB-	denotes	)
T3887	8296-8303	NN	denotes	subtype
T3886	8294-8295	NNP	denotes	E
T3885	8293-8294	-LRB-	denotes	(
T3884	8283-8292	NNP	denotes	HIV-1KR25
T3883	8279-8282	CC	denotes	and
T3882	8277-8278	,	denotes	,
T3881	8266-8277	NNP	denotes	HIV-192TH51
T3880	8264-8265	,	denotes	,
T3879	8253-8264	NNP	denotes	HIV-192TH53
T3878	8251-8252	,	denotes	,
T3877	8240-8251	NNP	denotes	HIV-193TH64
T3876	8238-8239	,	denotes	,
T3875	8237-8238	-RRB-	denotes	)
T3874	8230-8237	NN	denotes	subtype
T3873	8228-8229	NNP	denotes	C
T3872	8227-8228	-LRB-	denotes	(
T2882	6301-6302	.	denotes	.
T2881	6295-6301	NNP	denotes	Boston
T2880	6292-6294	IN	denotes	in
T2879	6283-6291	NN	denotes	Hospital
T2878	6280-6282	POS	denotes	's
T2877	6272-6280	NNP	denotes	Children
T2876	6269-6271	IN	denotes	of
T2875	6264-6268	NNP	denotes	Bank
T2874	6258-6263	NNP	denotes	Blood
T2873	6254-6257	DT	denotes	the
T3435	7666-7667	.	denotes	.
T3434	7663-7666	NNP	denotes	FCS
T3433	7661-7662	NN	denotes	%
T3432	7659-7661	CD	denotes	10
T3431	7654-7658	IN	denotes	with
T3430	7641-7653	VBN	denotes	supplemented
T3429	7639-7640	-RRB-	denotes	)
T3428	7621-7639	NN	denotes	www.invitrogen.com
T3427	7619-7620	,	denotes	,
T3426	7614-7619	NNP	denotes	Gibco
T3425	7613-7614	-LRB-	denotes	(
T3424	7611-7612	-RRB-	denotes	)
T3423	7607-7611	NNP	denotes	DMEM
T3422	7606-7607	-LRB-	denotes	(
T3421	7599-7605	NN	denotes	medium
T3420	7596-7598	POS	denotes	's
T3419	7591-7596	NNP	denotes	Eagle
T3418	7582-7590	NNP	denotes	Modified
T3417	7579-7581	POS	denotes	's
T3416	7571-7579	NNP	denotes	Dulbecco
T3415	7568-7570	IN	denotes	in
T3414	7557-7567	VBN	denotes	maintained
T3413	7552-7556	VBD	denotes	were
T3412	7548-7551	CC	denotes	and
T3411	7546-7547	-RRB-	denotes	)
T3410	7534-7546	NNP	denotes	www.atcc.org
T3409	7533-7534	-LRB-	denotes	(
T3408	7528-7532	NNP	denotes	ATCC
T3407	7523-7527	IN	denotes	from
T3406	7514-7522	VBN	denotes	obtained
T3405	7509-7513	VBD	denotes	were
T3404	7503-7508	NNS	denotes	cells
T3403	7498-7502	CD	denotes	293T
T3402	7496-7497	.	denotes	.
T3401	7495-7496	-RRB-	denotes	)
T3400	7479-7495	NN	denotes	www.lonzabio.com
T3399	7477-7478	,	denotes	,
T3398	7465-7477	NNP	denotes	BioWhittaker
T3397	7464-7465	-LRB-	denotes	(
T3396	7460-7463	NNP	denotes	FCS
T3395	7458-7459	NN	denotes	%
T3394	7456-7458	CD	denotes	10
T3393	7451-7455	IN	denotes	with
T3392	7438-7450	VBN	denotes	supplemented
T3391	7431-7437	JJ	denotes	medium
T3390	7426-7430	CD	denotes	1640
T3389	7421-7425	NNP	denotes	RPMI
T3388	7418-7420	IN	denotes	in
T3387	7409-7417	VBN	denotes	cultured
T3386	7405-7408	CC	denotes	and
T3385	7403-7404	-RRB-	denotes	)
T3384	7391-7403	NNP	denotes	www.atcc.org
T3383	7390-7391	-LRB-	denotes	(
T3382	7385-7389	NNP	denotes	ATCC
T3381	7380-7384	IN	denotes	from
T3380	7371-7379	VBN	denotes	obtained
T3379	7366-7370	VBD	denotes	were
T3378	7360-7365	NNS	denotes	cells
T3377	7354-7359	CD	denotes	THP-1
T3376	7352-7353	.	denotes	.
T3375	7343-7352	NN	denotes	cytometry
T2872	6249-6253	IN	denotes	from
T2871	6240-6248	VBD	denotes	obtained
T2870	6237-6239	PRP	denotes	we
T2869	6231-6236	WDT	denotes	which
T2868	6229-6230	,	denotes	,
T2867	6228-6229	-RRB-	denotes	)
T2866	6224-6228	NNP	denotes	PBMC
T2865	6222-6223	,	denotes	,
T2864	6217-6222	NNS	denotes	cells
T2863	6205-6216	NN	denotes	mononuclear
T2862	6199-6204	NN	denotes	blood
T2861	6188-6198	JJ	denotes	peripheral
T2860	6187-6188	-LRB-	denotes	(
T2859	6181-6186	NNS	denotes	cells
T2858	6175-6180	NN	denotes	blood
T2857	6165-6174	VBN	denotes	discarded
T2856	6159-6164	JJ	denotes	human
T2855	6146-6158	JJ	denotes	unidentified
T2854	6141-6145	VBD	denotes	used
T2853	6138-6140	PRP	denotes	we
T2852	6130-6137	NNS	denotes	studies
T2851	6126-6129	PRP$	denotes	our
T2850	6123-6125	IN	denotes	In
T2849	6113-6122	NN	denotes	statement
T2848	6106-6112	NNS	denotes	Ethics
T2624	5809-5813	NN	denotes	gene
T2623	5803-5808	NNP	denotes	NFAT5
T2622	5793-5802	VBZ	denotes	increases
T2621	5783-5792	NN	denotes	infection
T2620	5779-5782	NNP	denotes	MTb
T2619	5774-5778	IN	denotes	that
T2618	5762-5773	VBP	denotes	demonstrate
T2617	5759-5761	PRP	denotes	we
T2616	5757-5758	,	denotes	,
T2615	5749-5757	RB	denotes	Moreover
T2614	5747-5748	.	denotes	.
T2613	5743-5747	NNP	denotes	PBMC
T2612	5739-5742	CC	denotes	and
T2611	5735-5738	NNP	denotes	MDM
T2610	5729-5734	JJ	denotes	human
T2609	5726-5728	IN	denotes	in
T2608	5714-5725	NN	denotes	replication
T2607	5708-5713	JJ	denotes	HIV-1
T2606	5705-5707	IN	denotes	of
T2605	5693-5704	NN	denotes	stimulation
T2604	5681-5692	JJ	denotes	MTb-induced
T2603	5671-5680	JJ	denotes	efficient
T2602	5667-5670	IN	denotes	for
T2601	5656-5666	NN	denotes	importance
T2600	5648-5655	JJ	denotes	crucial
T2599	5645-5647	IN	denotes	of
T2598	5641-5644	VBP	denotes	are
T2597	5636-5640	NN	denotes	site
T2596	5628-5635	JJ	denotes	binding
T2595	5620-5627	JJ	denotes	cognate
T2594	5616-5619	PRP$	denotes	its
T2593	5612-5615	CC	denotes	and
T2592	5606-5611	CD	denotes	NFAT5
T2591	5601-5605	IN	denotes	that
T2590	5596-5600	VBP	denotes	show
T2589	5593-5595	PRP	denotes	we
T2588	5591-5592	,	denotes	,
T2587	5587-5591	RB	denotes	Here
T2586	5585-5586	.	denotes	.
T2585	5584-5585	-RRB-	denotes	)
T2584	5580-5584	NNP	denotes	PBMC
T2583	5579-5580	-LRB-	denotes	(
T2582	5573-5578	NNS	denotes	cells
T2581	5561-5572	NN	denotes	mononuclear
T2580	5555-5560	NN	denotes	blood
T2579	5544-5554	JJ	denotes	peripheral
T2578	5540-5543	CC	denotes	and
T2577	5530-5539	NNS	denotes	monocytes
T2576	5527-5529	IN	denotes	in
T2575	5519-5526	NN	denotes	pathway
T2574	5506-5518	JJ	denotes	TLR-mediated
T2573	5504-5505	DT	denotes	a
T2572	5500-5503	IN	denotes	via
T2571	5488-5499	NN	denotes	replication
T2570	5482-5487	JJ	denotes	HIV-1
T2569	5479-5481	IN	denotes	of
T2568	5468-5478	NN	denotes	activation
T2479	5259-5260	.	denotes	.
T2478	5258-5259	NNP	denotes	]
T2477	5256-5258	CD	denotes	31
T2476	5255-5256	NNP	denotes	[
T2475	5253-5254	-RRB-	denotes	)
T2474	5250-5253	NNP	denotes	MDM
T2473	5249-5250	-LRB-	denotes	(
T2472	5237-5248	NNS	denotes	macrophages
T2471	5220-5236	JJ	denotes	monocyte-derived
T2470	5212-5219	JJ	denotes	primary
T2469	5206-5211	JJ	denotes	human
T2468	5203-5205	IN	denotes	in
T2467	5194-5202	VBZ	denotes	isolates
T2466	5192-5193	NNP	denotes	E
T2465	5188-5191	CC	denotes	and
T2464	5186-5187	,	denotes	,
T2463	5185-5186	NNP	denotes	C
T2462	5183-5184	,	denotes	,
T2461	5182-5183	NNP	denotes	B
T2460	5174-5181	NN	denotes	subtype
T2459	5168-5173	NN	denotes	HIV-1
T2458	5153-5167	JJ	denotes	representative
T2457	5150-5152	IN	denotes	of
T2456	5138-5149	NN	denotes	replication
T2455	5125-5137	JJ	denotes	constitutive
T2454	5121-5124	IN	denotes	for
T2453	5112-5120	VBN	denotes	required
T2452	5109-5111	VBZ	denotes	is
T2451	5105-5108	CC	denotes	and
T2450	5101-5104	NNP	denotes	LTR
T2449	5097-5100	DT	denotes	the
T2448	5094-5096	IN	denotes	in
T2447	5087-5093	NNS	denotes	motifs
T2446	5079-5086	JJ	denotes	binding
T2445	5073-5078	NN	denotes	NF-κB
T2444	5068-5072	NN	denotes	core
T2443	5064-5067	DT	denotes	the
T2442	5055-5063	VBZ	denotes	overlaps
T2441	5050-5054	NN	denotes	site
T2440	5044-5049	CD	denotes	NFAT5
T2439	5039-5043	DT	denotes	This
T2438	5037-5038	.	denotes	.
T2437	5033-5037	NNPS	denotes	LTRs
T2436	5029-5032	NNP	denotes	SIV
T2435	5025-5028	CC	denotes	and
T4000	8912-8917	IN	denotes	after
T3999	8906-8911	NNS	denotes	hours
T3998	8903-8905	CD	denotes	16
T3997	8899-8902	IN	denotes	for
T3996	8897-8898	NNP	denotes	C
T3995	8896-8897	CD	denotes	°
T3994	8894-8896	CD	denotes	37
T3993	8891-8893	IN	denotes	at
T3992	8881-8890	VBN	denotes	incubated
T3991	8876-8880	VBD	denotes	were
T3990	8870-8875	NNS	denotes	Cells
T3989	8868-8869	.	denotes	.
T3988	8867-8868	-RRB-	denotes	)
T3987	8853-8867	NN	denotes	www.qiagen.com
T3986	8851-8852	:	denotes	;
T3985	8845-8851	NNP	denotes	Qiagen
T3984	8844-8845	-LRB-	denotes	(
T2434	5019-5024	NNP	denotes	HIV-2
T2433	5016-5018	IN	denotes	in
T2432	5006-5015	VBN	denotes	conserved
T2431	5001-5005	RB	denotes	also
T2430	4998-5000	VBZ	denotes	is
T2429	4994-4997	CC	denotes	and
T2428	4992-4993	,	denotes	,
T2427	4984-4992	NNS	denotes	subtypes
T2426	4978-4983	JJ	denotes	HIV-1
T2425	4974-4977	DT	denotes	all
T2424	4967-4973	IN	denotes	across
T2423	4957-4966	VBN	denotes	conserved
T2422	4950-4956	RB	denotes	highly
T2421	4947-4949	VBZ	denotes	is
T2420	4942-4946	WDT	denotes	that
T2419	4938-4941	NNP	denotes	LTR
T2418	4932-4937	NNP	denotes	HIV-1
T2417	4928-4931	DT	denotes	the
T2416	4921-4927	IN	denotes	within
T2415	4916-4920	NN	denotes	site
T2414	4914-4915	DT	denotes	a
T2413	4911-4913	TO	denotes	to
T2412	4905-4910	VBZ	denotes	binds
T2411	4903-4904	,	denotes	,
T2410	4902-4903	-RRB-	denotes	)
T2409	4896-4902	NNP	denotes	TonEBP
T2245	4112-4117	VBN	denotes	shown
T2244	4107-4111	VBN	denotes	been
T2243	4102-4106	VBP	denotes	have
T2242	4096-4101	WDT	denotes	which
T2241	4094-4095	,	denotes	,
T2240	4086-4094	NNS	denotes	families
T2239	4081-4085	JJ	denotes	AP-1
T2238	4077-4080	CC	denotes	and
T2237	4071-4076	NN	denotes	NF-κB
T2236	4067-4070	DT	denotes	the
T2235	4057-4066	VBG	denotes	including
T2234	4055-4056	,	denotes	,
T2233	4047-4055	NNS	denotes	families
T2232	4040-4046	NN	denotes	factor
T2231	4026-4039	NN	denotes	transcription
T2230	4017-4025	JJ	denotes	specific
T2229	4014-4016	IN	denotes	of
T2228	4003-4013	NN	denotes	activation
T2227	3996-4002	VB	denotes	induce
T2226	3985-3995	RB	denotes	ultimately
T2225	3983-3984	,	denotes	,
T2224	3979-3983	NN	denotes	turn
T2223	3976-3978	IN	denotes	in
T2222	3974-3975	,	denotes	,
T2221	3970-3974	IN	denotes	that
T2220	3962-3969	VBZ	denotes	kinases
T2219	3960-3961	-RRB-	denotes	)
T2218	3957-3960	NNP	denotes	MAP
T2217	3956-3957	-LRB-	denotes	(
T2216	3948-3955	NN	denotes	protein
T2215	3930-3947	JJ	denotes	mitogen-activated
T2214	3926-3929	CC	denotes	and
T2213	3924-3925	-RRB-	denotes	)
T2212	3921-3924	NNP	denotes	IKK
T2211	3920-3921	-LRB-	denotes	(
T2210	3913-3919	NN	denotes	kinase
T2209	3909-3912	NNP	denotes	IκB
T2208	3899-3908	NNS	denotes	activates
T2207	3893-3898	CD	denotes	TRAF6
T2206	3891-3892	.	denotes	.
T2205	3890-3891	-RRB-	denotes	)
T2204	3889-3890	NNP	denotes	]
T2203	3887-3889	CD	denotes	24
T2202	3886-3887	NNP	denotes	[
T2201	3884-3885	,	denotes	,
T2200	3883-3884	NNP	denotes	]
T2199	3881-3883	CD	denotes	10
T2198	3880-3881	NNP	denotes	[
T2197	3877-3879	IN	denotes	in
T2196	3868-3876	VBN	denotes	reviewed
T2195	3866-3867	,	denotes	,
T2194	3865-3866	NNP	denotes	]
T2193	3863-3865	CD	denotes	23
T2192	3862-3863	NNP	denotes	[
T2191	3860-3861	NNP	denotes	]
T2190	3858-3860	CD	denotes	21
T2189	3857-3858	NNP	denotes	[
T2188	3856-3857	-LRB-	denotes	(
T2187	3850-3855	NNP	denotes	TRAF6
T2186	3843-3849	NN	denotes	ligase
T2185	3833-3842	NN	denotes	ubiquitin
T2184	3829-3832	DT	denotes	the
T2183	3825-3828	CC	denotes	and
T2182	3823-3824	,	denotes	,
T2181	3818-3823	NNP	denotes	IRAK4
T2180	3814-3817	CC	denotes	and
T2179	3808-3813	NNP	denotes	IRAK1
T2178	3798-3807	VBG	denotes	including
T2177	3796-3797	,	denotes	,
T2176	3789-3796	NNS	denotes	kinases
T2175	3781-3788	JJ	denotes	several
T2174	3778-3780	IN	denotes	of
T2173	3766-3777	NN	denotes	recruitment
T2172	3755-3765	JJ	denotes	subsequent
T2171	3751-3754	DT	denotes	the
T2170	3747-3750	CC	denotes	and
T2169	3741-3746	NNP	denotes	MyD88
T2168	3733-3740	NN	denotes	protein
T2167	3725-3732	NN	denotes	adaptor
T2166	3721-3724	DT	denotes	the
T2165	3718-3720	IN	denotes	of
T2164	3707-3717	NN	denotes	engagement
T2163	3704-3706	IN	denotes	in
T2162	3696-3703	NNS	denotes	results
T2161	3691-3695	CD	denotes	TLR2
T2160	3688-3690	IN	denotes	of
T2159	3677-3687	NNP	denotes	Engagement
T2158	3675-3676	.	denotes	.
T2157	3674-3675	NNP	denotes	]
T2156	3672-3674	CD	denotes	20
T2155	3671-3672	NNP	denotes	[
T2154	3669-3670	NNP	denotes	]
T2153	3667-3669	CD	denotes	16
T2152	3666-3667	NNP	denotes	[
T2151	3664-3665	CD	denotes	2
T2150	3662-3663	-RRB-	denotes	)
T2149	3659-3662	NNP	denotes	TLR
T2148	3658-3659	-LRB-	denotes	(
T2147	3649-3657	NN	denotes	receptor
T2146	3639-3648	JJ	denotes	toll-like
T2145	3636-3638	VBZ	denotes	is
T2144	3626-3635	NN	denotes	infection
T2143	3622-3625	NNP	denotes	MTb
T2142	3619-3621	IN	denotes	by
T2141	3609-3618	VBN	denotes	triggered
T2140	3603-3608	NNS	denotes	cells
T2139	3593-3602	JJ	denotes	monocytic
T2138	3590-3592	IN	denotes	on
T2137	3586-3589	NNP	denotes	PRR
T2136	3578-3585	JJ	denotes	primary
T2135	3574-3577	DT	denotes	The
T2134	3572-3573	.	denotes	.
T2133	3571-3572	-RRB-	denotes	)
T2132	3570-3571	NNP	denotes	]
T2131	3569-3570	CD	denotes	8
T2130	3568-3569	NN	denotes	[
T2129	3565-3567	IN	denotes	in
T2128	3556-3564	VBN	denotes	reviewed
T2127	3554-3555	,	denotes	,
T2126	3553-3554	NNP	denotes	]
T2125	3551-3553	CD	denotes	15
T2124	3550-3551	NNP	denotes	[
T2123	3548-3549	NNP	denotes	]
T2122	3546-3548	CD	denotes	11
T2121	3545-3546	NNP	denotes	[
T2120	3544-3545	-LRB-	denotes	(
T2119	3534-3543	NN	denotes	infection
T2118	3531-3533	NN	denotes	TB
T2117	3523-3530	JJ	denotes	primary
T2116	3520-3522	TO	denotes	to
T2115	3505-3519	NN	denotes	susceptibility
T2114	3495-3504	VBN	denotes	increased
T2113	3492-3494	IN	denotes	as
T2112	3487-3491	RB	denotes	well
T2111	3484-3486	RB	denotes	as
T2110	3482-3483	,	denotes	,
T2109	3470-3482	NN	denotes	reactivation
T2108	3466-3469	NNP	denotes	MTb
T2107	3459-3465	NN	denotes	latent
T2106	3456-3458	IN	denotes	of
T2105	3450-3455	NN	denotes	cause
T2104	3444-3449	JJ	denotes	major
T2103	3442-3443	DT	denotes	a
T2102	3439-3441	VBZ	denotes	is
T2101	3432-3438	PRP	denotes	itself
T2100	3422-3431	NN	denotes	infection
T2099	3416-3421	JJ	denotes	HIV-1
T2098	3411-3415	IN	denotes	with
T2097	3400-3410	VBN	denotes	associated
T2096	3389-3399	NN	denotes	compromise
T2095	3382-3388	JJ	denotes	immune
T2094	3370-3381	JJ	denotes	progressive
T2093	3366-3369	DT	denotes	the
T2092	3364-3365	,	denotes	,
T2091	3353-3364	RB	denotes	Furthermore
T2090	3351-3352	.	denotes	.
T2089	3350-3351	-RRB-	denotes	)
T2088	3349-3350	NNP	denotes	]
T2087	3347-3349	CD	denotes	10
T2086	3346-3347	NNP	denotes	[
T2085	3344-3345	NNP	denotes	]
T2084	3343-3344	CD	denotes	8
T2083	3342-3343	NN	denotes	[
T2082	3339-3341	IN	denotes	in
T2081	3330-3338	VBN	denotes	reviewed
T2080	3328-3329	,	denotes	,
T2079	3327-3328	NNP	denotes	]
T2078	3326-3327	CD	denotes	7
T2077	3325-3326	NNP	denotes	[
T2076	3324-3325	-LRB-	denotes	(
T2075	3319-3323	NNP	denotes	AIDS
T2074	3316-3318	TO	denotes	to
T2073	3304-3315	NN	denotes	progression
T2072	3300-3303	CC	denotes	and
T2071	3295-3299	NN	denotes	loss
T2070	3290-3294	NN	denotes	cell
T2069	3288-3289	NNP	denotes	T
T2068	3284-3287	NNP	denotes	CD4
T2067	3274-3283	VBN	denotes	expedited
T2066	3272-3273	,	denotes	,
T2065	3268-3272	NN	denotes	turn
T2064	3265-3267	IN	denotes	in
T2063	3263-3264	,	denotes	,
T2062	3260-3263	CC	denotes	and
T2061	3254-3259	NNS	denotes	loads
T2060	3248-3253	JJ	denotes	viral
T2059	3241-3247	JJR	denotes	higher
T2058	3238-3240	TO	denotes	to
T2057	3232-3237	VBZ	denotes	leads
T2056	3221-3231	RB	denotes	ultimately
T2666	6080-6081	.	denotes	.
T2665	6069-6080	NN	denotes	replication
T2664	6063-6068	JJ	denotes	HIV-1
T2663	6060-6062	IN	denotes	of
T2662	6048-6059	NN	denotes	enhancement
T2661	6036-6047	JJ	denotes	MTb-induced
T2660	6033-6035	IN	denotes	of
T2659	6023-6032	NN	denotes	modulator
T2658	6014-6022	JJ	denotes	critical
T2657	6012-6013	DT	denotes	a
T2656	6009-6011	VBZ	denotes	is
T2655	6003-6008	NNP	denotes	NFAT5
T2654	5998-6002	DT	denotes	that
T2653	5986-5997	VB	denotes	demonstrate
T2652	5982-5985	CC	denotes	and
T2651	5980-5981	,	denotes	,
T2650	5972-5980	NN	denotes	response
T2649	5965-5971	JJ	denotes	immune
T2648	5958-5964	NN	denotes	innate
T2647	5945-5957	JJ	denotes	PRR-mediated
T2646	5941-5944	DT	denotes	the
T2645	5938-5940	TO	denotes	to
T2644	5927-5937	NN	denotes	expression
T2643	5921-5926	CD	denotes	NFAT5
T2642	5918-5920	IN	denotes	of
T2641	5910-5917	NNS	denotes	stimuli
T2640	5904-5909	VBN	denotes	known
T2639	5900-5903	DT	denotes	the
T2638	5893-5899	VBP	denotes	expand
T2637	5885-5892	NNS	denotes	results
T2636	5879-5884	DT	denotes	these
T2635	5877-5878	,	denotes	,
T2634	5873-5877	RB	denotes	Thus
T2633	5871-5872	.	denotes	.
T2632	5865-5871	NN	denotes	manner
T2631	5849-5864	JJ	denotes	MyD88-dependent
T2630	5847-5848	DT	denotes	a
T2629	5844-5846	IN	denotes	in
T2628	5834-5843	NNS	denotes	monocytes
T2627	5828-5833	JJ	denotes	human
T2626	5825-5827	IN	denotes	in
T2625	5814-5824	NN	denotes	expression
T2055	3212-3220	NNS	denotes	pathways
T2054	3200-3211	JJ	denotes	MTb-induced
T2053	3194-3199	DT	denotes	these
T2052	3190-3193	IN	denotes	via
T2051	3178-3189	NN	denotes	replication
T2050	3172-3177	NNP	denotes	HIV-1
T2049	3169-3171	IN	denotes	of
T2048	3158-3168	NNP	denotes	Activation
T2047	3156-3157	.	denotes	.
T2046	3155-3156	NNP	denotes	]
T2045	3154-3155	CD	denotes	6
T2044	3153-3154	NN	denotes	[
T2043	3151-3152	NNP	denotes	]
T2042	3150-3151	CD	denotes	4
T2041	3149-3150	NNP	denotes	[
T2040	3145-3148	NNP	denotes	LTR
T2039	3139-3144	NNP	denotes	HIV-1
T2038	3135-3138	DT	denotes	the
T2037	3132-3134	IN	denotes	of
T2036	3121-3131	NN	denotes	activation
T2035	3105-3120	JJ	denotes	transcriptional
T2034	3102-3104	IN	denotes	in
T2033	3095-3101	VBP	denotes	result
T2032	3090-3094	RB	denotes	also
T2031	3085-3089	WDT	denotes	that
T2030	3079-3084	NNS	denotes	cells
T2408	4893-4895	IN	denotes	as
T2407	4887-4892	VBN	denotes	known
T2406	4882-4886	RB	denotes	also
T2405	4881-4882	-LRB-	denotes	(
T2404	4875-4880	NNP	denotes	NFAT5
T2403	4873-4874	,	denotes	,
T2402	4867-4873	NN	denotes	family
T2401	4865-4866	-RRB-	denotes	)
T2400	4861-4865	NNP	denotes	NFAT
T2399	4860-4861	-LRB-	denotes	(
T2398	4854-4859	NNS	denotes	cells
T2397	4852-4853	NNP	denotes	T
T2396	4842-4851	VBN	denotes	activated
T2395	4839-4841	IN	denotes	of
T2394	4832-4838	NN	denotes	factor
T2393	4824-4831	JJ	denotes	nuclear
T2392	4820-4823	DT	denotes	the
T2391	4817-4819	IN	denotes	of
T2390	4810-4816	NN	denotes	member
T2389	4799-4809	JJ	denotes	primordial
T2388	4794-4798	RBS	denotes	most
T2387	4790-4793	DT	denotes	the
T2386	4785-4789	IN	denotes	that
T2385	4778-4784	VBD	denotes	showed
T2384	4767-4777	RB	denotes	previously
T2383	4764-4766	PRP	denotes	We
T2382	4762-4763	.	denotes	.
T2381	4761-4762	NNP	denotes	]
T2380	4759-4761	CD	denotes	30
T2379	4758-4759	NNP	denotes	[
T2378	4756-4757	NNP	denotes	]
T2377	4754-4756	CD	denotes	28
T2376	4753-4754	NNP	denotes	[
T2375	4751-4752	,	denotes	,
T2374	4750-4751	NNP	denotes	]
T2373	4749-4750	CD	denotes	1
T2372	4748-4749	NNP	denotes	[
T2371	4735-4747	RB	denotes	respectively
T2370	4733-4734	,	denotes	,
T2369	4728-4733	NNS	denotes	sites
T2368	4720-4727	JJ	denotes	binding
T2367	4714-4719	JJ	denotes	NF-κB
T2366	4710-4713	CD	denotes	one
T2365	4706-4709	CC	denotes	and
T2364	4700-4705	CD	denotes	three
T2363	4695-4699	VBP	denotes	have
T2362	4693-4694	,	denotes	,
T2361	4689-4693	NNP	denotes	Asia
T2360	4679-4688	JJ	denotes	southeast
T2359	4675-4678	CC	denotes	and
T2358	4668-4674	NNP	denotes	Africa
T2357	4656-4667	JJ	denotes	sub-Saharan
T2356	4644-4655	JJ	denotes	TB-burdened
T2355	4641-4643	IN	denotes	in
T2354	4622-4640	RB	denotes	disproportionately
T2353	4615-4621	VBN	denotes	spread
T2352	4610-4614	VBP	denotes	have
T2351	4604-4609	WDT	denotes	which
T2350	4602-4603	,	denotes	,
T2349	4601-4602	NNP	denotes	E
T2348	4597-4600	CC	denotes	and
T2347	4595-4596	NNP	denotes	C
T2346	4586-4594	VBZ	denotes	subtypes
T2345	4580-4585	NNP	denotes	HIV-1
T2344	4578-4579	,	denotes	,
T2343	4570-4578	NN	denotes	contrast
T2342	4567-4569	IN	denotes	By
T2341	4565-4566	.	denotes	.
T2340	4562-4565	NNP	denotes	LTR
T2339	4558-4561	PRP$	denotes	its
T2338	4555-4557	IN	denotes	in
T2337	4548-4554	NNS	denotes	motifs
T2336	4542-4547	NN	denotes	NF-κB
T2335	4535-4541	NN	denotes	tandem
T2334	4531-4534	CD	denotes	two
T2333	4527-4530	VBZ	denotes	has
T2332	4525-4526	,	denotes	,
T2331	4516-4525	NNP	denotes	Australia
T2330	4512-4515	CC	denotes	and
T2329	4510-4511	,	denotes	,
T2328	4505-4510	NNP	denotes	Japan
T2327	4503-4504	,	denotes	,
T2326	4497-4503	NNP	denotes	Europe
T2325	4495-4496	,	denotes	,
T2324	4487-4495	NNPS	denotes	Americas
T2323	4483-4486	DT	denotes	the
T2322	4480-4482	IN	denotes	in
T2321	4470-4479	NN	denotes	infection
T2320	4467-4469	IN	denotes	of
T2319	4461-4466	NN	denotes	cause
T2318	4453-4460	JJ	denotes	primary
T2317	4449-4452	DT	denotes	the
T2316	4445-4448	CC	denotes	and
T2315	4437-4444	NN	denotes	subtype
T2314	4431-4436	JJ	denotes	viral
T2313	4417-4430	VBN	denotes	characterized
T2312	4410-4416	RB	denotes	highly
T2311	4405-4409	RBS	denotes	most
T2310	4401-4404	DT	denotes	the
T2309	4399-4400	,	denotes	,
T2308	4398-4399	NNP	denotes	B
T2307	4390-4397	NN	denotes	subtype
T2306	4384-4389	JJ	denotes	HIV-1
T2305	4382-4383	,	denotes	,
T2304	4375-4382	NN	denotes	example
T2303	4371-4374	IN	denotes	For
T2302	4369-4370	.	denotes	.
T2301	4364-4369	NNS	denotes	sites
T2300	4356-4363	JJ	denotes	binding
T2299	4346-4355	NN	denotes	activator
T2298	4343-4345	IN	denotes	of
T2297	4330-4342	NN	denotes	organization
T2296	4326-4329	CC	denotes	and
T2295	4319-4325	NN	denotes	number
T2294	4315-4318	DT	denotes	the
T2293	4312-4314	TO	denotes	to
T2292	4304-4311	NN	denotes	respect
T2291	4299-4303	IN	denotes	with
T2290	4292-4298	JJ	denotes	unique
T2289	4289-4291	VBZ	denotes	is
T2288	4281-4288	NN	denotes	subtype
T2287	4276-4280	DT	denotes	each
T2286	4273-4275	IN	denotes	of
T2285	4269-4272	NNP	denotes	LTR
T2284	4265-4268	DT	denotes	the
T2283	4261-4264	CC	denotes	and
T2282	4259-4260	,	denotes	,
T2281	4251-4259	NNS	denotes	subtypes
T2280	4243-4250	JJ	denotes	several
T2279	4233-4242	VBZ	denotes	comprises
T2278	4227-4232	JJ	denotes	HIV-1
T2277	4225-4226	,	denotes	,
T2276	4218-4225	RB	denotes	Notably
T2275	4216-4217	.	denotes	.
T2274	4215-4216	-RRB-	denotes	)
T2273	4214-4215	NNP	denotes	]
T2272	4212-4214	CD	denotes	10
T2271	4211-4212	NNP	denotes	[
T2270	4208-4210	IN	denotes	in
T2269	4199-4207	VBN	denotes	reviewed
T2268	4197-4198	,	denotes	,
T2267	4196-4197	NNP	denotes	]
T2266	4194-4196	CD	denotes	27
T2265	4193-4194	NNP	denotes	[
T2264	4191-4192	NNP	denotes	]
T2263	4189-4191	CD	denotes	25
T2262	4188-4189	NNP	denotes	[
T2261	4186-4187	,	denotes	,
T2260	4185-4186	NNP	denotes	]
T2259	4183-4185	CD	denotes	22
T2258	4182-4183	NNP	denotes	[
T2257	4181-4182	-LRB-	denotes	(
T2256	4167-4180	NN	denotes	transcription
T2255	4163-4166	PRP$	denotes	its
T2254	4157-4162	VB	denotes	drive
T2253	4154-4156	TO	denotes	to
T2252	4150-4153	CC	denotes	and
T2251	4146-4149	NNP	denotes	LTR
T2250	4140-4145	NNP	denotes	HIV-1
T2249	4136-4139	DT	denotes	the
T2248	4131-4135	IN	denotes	with
T2247	4121-4130	VB	denotes	associate
T2246	4118-4120	TO	denotes	to
T2028	3065-3068	CC	denotes	and
T2027	3059-3064	NNS	denotes	cells
T2026	3057-3058	NNP	denotes	T
T2025	3053-3056	NNP	denotes	CD4
T2024	3050-3052	IN	denotes	in
T2023	3041-3049	NNS	denotes	pathways
T2022	3028-3040	NN	denotes	transduction
T2021	3021-3027	NN	denotes	signal
T2020	3012-3020	VBP	denotes	activate
T2019	3002-3011	NN	denotes	infection
T2018	2998-3001	NNP	denotes	MTb
T2017	2995-2997	TO	denotes	to
T2016	2986-2994	NN	denotes	response
T2015	2983-2985	IN	denotes	in
T2014	2978-2982	NN	denotes	host
T2013	2972-2977	JJ	denotes	human
T2012	2968-2971	DT	denotes	the
T2011	2965-2967	IN	denotes	by
T2010	2956-2964	VBN	denotes	produced
T2009	2945-2955	NNS	denotes	chemokines
T2008	2941-2944	CC	denotes	and
T2007	2931-2940	NNS	denotes	cytokines
T2006	2918-2930	JJ	denotes	inflammatory
T2005	2916-2917	,	denotes	,
T2004	2905-2916	RB	denotes	Furthermore
T2003	2903-2904	.	denotes	.
T2002	2902-2903	-RRB-	denotes	)
T2001	2901-2902	NNP	denotes	]
T2000	2900-2901	CD	denotes	3
T1999	2899-2900	NNP	denotes	[
T1998	2896-2898	IN	denotes	in
T1997	2887-2895	VBN	denotes	reviewed
T1996	2886-2887	-LRB-	denotes	(
T1995	2882-2885	NNP	denotes	LTR
T1994	2873-2881	JJ	denotes	proviral
T1993	2869-2872	DT	denotes	the
T1992	2864-2868	IN	denotes	from
T1991	2850-2863	NN	denotes	transcription
T1990	2843-2849	VBP	denotes	induce
T1989	2834-2842	RB	denotes	directly
T1988	2829-2833	WDT	denotes	that
T1987	2820-2828	NNS	denotes	cascades
T1986	2810-2819	VBG	denotes	signaling
T1985	2800-2809	VBZ	denotes	activates
T1984	2789-2799	NNS	denotes	phagocytes
T1983	2777-2788	NN	denotes	mononuclear
T1982	2774-2776	IN	denotes	on
T1981	2770-2773	NNP	denotes	MTb
T1980	2767-2769	IN	denotes	by
T1979	2765-2766	-RRB-	denotes	)
T1978	2761-2765	NNS	denotes	PRRs
T1977	2760-2761	-LRB-	denotes	(
T1976	2750-2759	NNS	denotes	receptors
T1975	2738-2749	NN	denotes	recognition
T1974	2729-2737	NN	denotes	pathogen
T1973	2726-2728	IN	denotes	of
T1972	2715-2725	NN	denotes	engagement
T1971	2708-2714	NNP	denotes	Direct
T1970	2706-2707	.	denotes	.
T1969	2705-2706	NNP	denotes	]
T1968	2704-2705	CD	denotes	2
T1967	2703-2704	NNP	denotes	[
T1966	2701-2702	,	denotes	,
T1965	2700-2701	NNP	denotes	]
T1964	2699-2700	CD	denotes	1
T1963	2698-2699	NN	denotes	[
T1962	2696-2697	-RRB-	denotes	)
T1961	2691-2696	NN	denotes	HIV-1
T1960	2690-2691	-LRB-	denotes	(
T1959	2688-2689	CD	denotes	1
T1958	2683-2687	NN	denotes	type
T1957	2677-2682	NN	denotes	virus
T1956	2660-2676	NN	denotes	immunodeficiency
T1955	2654-2659	JJ	denotes	human
T1954	2649-2653	IN	denotes	with
T1953	2640-2648	VBN	denotes	infected
T1952	2631-2639	NNS	denotes	patients
T1951	2628-2630	IN	denotes	in
T1950	2622-2627	NN	denotes	death
T1949	2619-2621	IN	denotes	of
T1948	2613-2618	NN	denotes	cause
T1947	2609-2612	CC	denotes	and
T1946	2596-2608	NN	denotes	co-infection
T1945	2589-2595	JJ	denotes	common
T1944	2584-2588	RBS	denotes	most
T1943	2580-2583	DT	denotes	the
T1942	2577-2579	VBZ	denotes	is
T1941	2575-2576	,	denotes	,
T1940	2574-2575	-RRB-	denotes	)
T1939	2572-2574	NN	denotes	TB
T1938	2571-2572	-LRB-	denotes	(
T1937	2558-2570	NN	denotes	tuberculosis
T1936	2555-2557	IN	denotes	of
T1935	2549-2554	NN	denotes	agent
T1934	2539-2548	JJ	denotes	causative
T1933	2535-2538	DT	denotes	the
T1932	2533-2534	,	denotes	,
T1931	2532-2533	-RRB-	denotes	)
T1930	2529-2532	NNP	denotes	MTb
T1929	2528-2529	-LRB-	denotes	(
T1928	2515-2527	NN	denotes	tuberculosis
T1927	2501-2514	NNP	denotes	Mycobacterium
T2029	3069-3078	JJ	denotes	monocytic
T754	1510-1511	.	denotes	.
T753	1500-1510	NN	denotes	expression
T752	1495-1499	NN	denotes	gene
T751	1492-1494	IN	denotes	of
T750	1484-1491	NN	denotes	control
T749	1471-1483	JJ	denotes	MTb-mediated
T748	1467-1470	CC	denotes	and
T747	1465-1466	:	denotes	-
T746	1462-1465	NNP	denotes	TLR
T745	1459-1461	IN	denotes	in
T744	1453-1458	NNP	denotes	NFAT5
T743	1449-1452	IN	denotes	for
T742	1444-1448	NN	denotes	role
T741	1436-1443	JJ	denotes	general
T740	1434-1435	DT	denotes	a
T739	1422-1433	VBP	denotes	demonstrate
T738	1417-1421	RB	denotes	also
T737	1408-1416	NNS	denotes	findings
T736	1402-1407	DT	denotes	These
T735	1400-1401	.	denotes	.
T734	1392-1400	NN	denotes	promoter
T733	1386-1391	JJ	denotes	viral
T732	1382-1385	DT	denotes	the
T731	1377-1381	IN	denotes	with
T730	1365-1376	NN	denotes	interaction
T729	1358-1364	JJ	denotes	direct
T728	1356-1357	DT	denotes	a
T727	1352-1355	IN	denotes	via
T726	1340-1351	NN	denotes	replication
T725	1334-1339	JJ	denotes	HIV-1
T724	1331-1333	IN	denotes	of
T723	1320-1330	NN	denotes	regulation
T722	1316-1319	NNP	denotes	MTb
T721	1313-1315	IN	denotes	in
T720	1308-1312	NN	denotes	role
T719	1300-1307	JJ	denotes	crucial
T718	1298-1299	DT	denotes	a
T717	1292-1297	VBZ	denotes	plays
T716	1286-1291	NNP	denotes	NFAT5
T715	1282-1285	CC	denotes	and
T714	1280-1281	,	denotes	,
T713	1270-1280	NN	denotes	expression
T712	1262-1269	NN	denotes	protein
T711	1258-1261	CC	denotes	and
T710	1253-1257	NN	denotes	gene
T709	1247-1252	NNP	denotes	NFAT5
T708	1239-1246	VBZ	denotes	induces
T707	1229-1238	NN	denotes	infection
T706	1225-1228	NNP	denotes	MTb
T705	1222-1224	TO	denotes	to
T704	1213-1221	NN	denotes	response
T703	1206-1212	JJ	denotes	immune
T702	1199-1205	NN	denotes	innate
T701	1195-1198	DT	denotes	the
T700	1193-1194	,	denotes	,
T699	1189-1193	RB	denotes	Thus
T698	1187-1188	.	denotes	.
T697	1177-1187	NN	denotes	expression
T696	1172-1176	NN	denotes	gene
T695	1166-1171	CD	denotes	NFAT5
T694	1154-1165	JJ	denotes	MTb-induced
T693	1145-1153	VBZ	denotes	inhibits
T692	1131-1144	RB	denotes	significantly
T691	1129-1130	,	denotes	,
T690	1124-1129	NNP	denotes	TRAF6
T689	1120-1123	CC	denotes	and
T688	1118-1119	,	denotes	,
T687	1113-1118	NNP	denotes	IRAK1
T686	1111-1112	,	denotes	,
T685	1106-1111	NNP	denotes	MyD88
T684	1096-1105	NNS	denotes	molecules
T683	1086-1095	VBG	denotes	signaling
T647	876-877	NNP	denotes	B
T646	868-875	NN	denotes	subtype
T645	862-867	NN	denotes	HIV-1
T644	852-861	JJ	denotes	R5-tropic
T643	849-851	IN	denotes	of
T642	840-848	NN	denotes	promoter
T641	834-839	JJ	denotes	viral
T640	830-833	DT	denotes	the
T639	827-829	IN	denotes	in
T638	822-826	NN	denotes	site
T637	814-821	JJ	denotes	binding
T636	808-813	NNP	denotes	NFAT5
T635	801-807	JJ	denotes	intact
T634	798-800	DT	denotes	an
T633	793-797	IN	denotes	that
T632	789-792	CC	denotes	and
T631	787-788	,	denotes	,
T630	781-787	NN	denotes	ligand
T629	779-780	-RRB-	denotes	)
T628	776-779	NNP	denotes	TLR
T627	775-776	-LRB-	denotes	(
T626	766-774	NN	denotes	receptor
T625	756-765	JJ	denotes	Toll-like
T624	754-755	DT	denotes	a
T623	751-753	VBZ	denotes	is
T622	745-750	WDT	denotes	which
T621	743-744	,	denotes	,
T620	740-743	NNP	denotes	MTb
T619	737-739	IN	denotes	by
T618	729-736	VBN	denotes	induced
T617	720-728	RB	denotes	strongly
T616	716-719	VBP	denotes	are
T615	705-715	NN	denotes	expression
T614	697-704	NN	denotes	protein
T613	693-696	CC	denotes	and
T612	688-692	NN	denotes	gene
T611	682-687	CD	denotes	NFAT5
T610	677-681	IN	denotes	that
T609	672-676	VBP	denotes	show
T608	669-671	PRP	denotes	We
T607	667-668	.	denotes	.
T606	656-667	NNS	denotes	macrophages
T605	644-655	JJ	denotes	co-infected
T604	641-643	IN	denotes	in
T603	629-640	NN	denotes	replication
T602	623-628	NNP	denotes	HIV-1
T601	612-622	VBN	denotes	stimulated
T600	611-612	:	denotes	-
T599	610-611	-RRB-	denotes	)
T598	607-610	NNP	denotes	MTb
T597	606-607	-LRB-	denotes	(
T596	593-605	NN	denotes	tuberculosis
T595	579-592	NNP	denotes	Mycobacterium
T594	570-578	VBZ	denotes	inhibits
T593	568-569	-RRB-	denotes	)
T592	564-568	NNS	denotes	RNAi
T591	563-564	-LRB-	denotes	(
T590	550-562	NN	denotes	interference
T589	546-549	NNP	denotes	RNA
T588	543-545	IN	denotes	by
T568	424-435	VBP	denotes	demonstrate
T567	421-423	PRP	denotes	we
T566	419-420	,	denotes	,
T565	415-419	RB	denotes	Here
T564	413-414	.	denotes	.
T563	409-413	NN	denotes	load
T562	403-408	JJ	denotes	viral
T561	400-402	IN	denotes	in
T560	391-399	NN	denotes	increase
T559	388-390	DT	denotes	an
T558	385-387	TO	denotes	to
T557	377-384	VBG	denotes	leading
T556	375-376	,	denotes	,
T555	364-375	NN	denotes	replication
T554	358-363	JJ	denotes	HIV-1
T553	348-357	VBP	denotes	stimulate
T552	343-347	WDT	denotes	that
T551	334-342	NNS	denotes	cascades
T550	324-333	VBG	denotes	signaling
T549	317-323	JJ	denotes	immune
T548	308-316	JJ	denotes	adaptive
T547	304-307	CC	denotes	and
T546	297-303	NN	denotes	innate
T545	286-296	VBG	denotes	activating
T544	283-285	IN	denotes	by
T543	273-282	NN	denotes	mortality
T542	263-272	VBN	denotes	increased
T541	260-262	TO	denotes	to
T540	248-259	VBZ	denotes	contributes
T539	239-247	NNS	denotes	patients
T538	227-238	VBD	denotes	co-infected
T537	223-226	NNP	denotes	HIV
T536	220-222	IN	denotes	in
T535	212-219	NN	denotes	disease
T534	210-211	-RRB-	denotes	)
T533	208-210	NN	denotes	TB
T532	207-208	-LRB-	denotes	(
T531	194-206	NNP	denotes	Tuberculosis
T3538	7800-7808	NN	denotes	Facility
T3537	7788-7799	NNP	denotes	Centralized
T3536	7784-7787	DT	denotes	The
T3535	7779-7783	IN	denotes	from
T3534	7770-7778	VBN	denotes	obtained
T3533	7765-7769	VBD	denotes	were
T3532	7753-7764	NNP	denotes	HIV-198IN22
T3529	7736-7747	NNP	denotes	HIV-198CH01
T3528	7734-7735	,	denotes	,
T3527	7723-7734	NNP	denotes	HIV-192TH53
T3526	7721-7722	,	denotes	,
T3525	7710-7721	NNP	denotes	HIV-192TH51
T3524	7708-7709	,	denotes	,
T3523	7697-7708	NNP	denotes	HIV-193TH64
T3522	7695-7696	,	denotes	,
T3521	7687-7695	NNP	denotes	HIV-1Lai
T3520	7685-7686	,	denotes	,
T3519	7677-7685	NNP	denotes	HIV-1Bal
T3518	7669-7676	NNPS	denotes	Viruses
T6249	13618-13622	NN	denotes	cell
T6248	13612-13617	NNP	denotes	Whole
T6247	13607-13611	NN	denotes	blot
T6246	13599-13606	JJ	denotes	Western
T3564	7952-7961	VBN	denotes	described
T3563	7949-7951	IN	denotes	as
T3562	7938-7948	NN	denotes	laboratory
T3561	7934-7937	PRP$	denotes	our
T3560	7931-7933	IN	denotes	in
T3559	7922-7930	VBN	denotes	isolated
T3558	7918-7921	VBD	denotes	was
T3557	7908-7917	CD	denotes	HIV-1KR25
T3556	7906-7907	.	denotes	.
T3555	7899-7906	NNP	denotes	Kingdom
T3554	7892-7898	NNP	denotes	United
T3553	7890-7891	,	denotes	,
T3552	7889-7890	-RRB-	denotes	)
T3551	7884-7889	NNP	denotes	NIBSC
T3550	7883-7884	-LRB-	denotes	(
T3549	7875-7882	NNP	denotes	Control
T3548	7871-7874	CC	denotes	and
T3547	7862-7870	NNP	denotes	Standard
T3546	7851-7861	NNP	denotes	Biological
T3545	7847-7850	IN	denotes	for
T3544	7837-7846	NNP	denotes	Institute
T3543	7828-7836	NNP	denotes	National
T3542	7826-7827	,	denotes	,
T3541	7818-7826	NNPS	denotes	Reagents
T3540	7813-7817	NNP	denotes	AIDS
T3539	7809-7812	IN	denotes	for
T3531	7749-7752	CC	denotes	and
T3530	7747-7748	,	denotes	,
T3569	7973-7974	.	denotes	.
T3568	7972-7973	NNP	denotes	]
T3567	7970-7972	CD	denotes	33
T3566	7969-7970	NNP	denotes	[
T3565	7962-7968	IN	denotes	before
T2509	5410-5420	VBD	denotes	speculated
T2508	5407-5409	PRP	denotes	we
T2507	5405-5406	,	denotes	,
T2506	5404-5405	NNP	denotes	]
T2505	5402-5404	CD	denotes	32
T2504	5401-5402	NNP	denotes	[
T2503	5399-5400	,	denotes	,
T2502	5390-5399	VBG	denotes	signaling
T2501	5384-5389	NNP	denotes	MyD88
T2500	5381-5383	IN	denotes	of
T2499	5370-5380	JJ	denotes	downstream
T2498	5367-5369	VBZ	denotes	is
T2497	5361-5366	WDT	denotes	which
T2496	5359-5360	,	denotes	,
T2495	5356-5359	CD	denotes	p38
T2494	5349-5355	NN	denotes	kinase
T2493	5345-5348	NNP	denotes	MAP
T2492	5341-5344	DT	denotes	the
T2491	5338-5340	IN	denotes	by
T2490	5328-5337	VBN	denotes	activated
T2489	5310-5327	RB	denotes	transcriptionally
T2488	5307-5309	VB	denotes	be
T2487	5304-5306	TO	denotes	to
T2486	5298-5303	VBN	denotes	shown
T2485	5293-5297	VBN	denotes	been
T2484	5282-5292	RB	denotes	previously
T2483	5278-5281	VBZ	denotes	has
T2482	5272-5277	NNP	denotes	NFAT5
T2481	5267-5271	IN	denotes	that
T2480	5261-5266	VBN	denotes	Given
T2567	5456-5467	JJ	denotes	MTb-induced
T2566	5453-5455	IN	denotes	in
T2565	5444-5452	VBN	denotes	involved
T2564	5441-5443	VB	denotes	be
T2563	5436-5440	RB	denotes	also
T2562	5432-5435	MD	denotes	may
T2561	5426-5431	NNP	denotes	NFAT5
T2560	5421-5425	IN	denotes	that
T4591	10198-10209	NN	denotes	HIV-198IN22
T4590	10195-10197	IN	denotes	of
T4589	10187-10194	VB	denotes	isolate
T4588	10179-10186	JJ	denotes	primary
T4587	10177-10178	DT	denotes	a
T4586	10172-10176	IN	denotes	with
T4585	10163-10171	VBN	denotes	infected
T4584	10158-10162	VBD	denotes	were
T4583	10153-10157	WDT	denotes	that
T4582	10148-10152	NNP	denotes	PBMC
T4581	10143-10147	IN	denotes	from
T4580	10133-10142	VBN	denotes	extracted
T3871	8218-8226	NNP	denotes	HIV-1CM9
T3870	8216-8217	,	denotes	,
T3869	8205-8216	NNP	denotes	HIV-198CH01
T3868	8203-8204	,	denotes	,
T3867	8192-8203	NNP	denotes	HIV-198IN22
T3866	8190-8191	,	denotes	,
T3865	8179-8190	NNP	denotes	HIV-198IN17
T3864	8177-8178	,	denotes	,
T3863	8176-8177	-RRB-	denotes	)
T3862	8169-8176	NN	denotes	subtype
T3861	8167-8168	NNP	denotes	B
T3860	8166-8167	-LRB-	denotes	(
T3859	8157-8165	JJ	denotes	HIV-1Bal
T3858	8155-8156	,	denotes	,
T3857	8147-8155	JJ	denotes	HIV-1Lai
T3856	8144-8146	IN	denotes	of
T3855	8139-8143	NN	denotes	site
T3854	8128-8138	NN	denotes	initiation
T3853	8112-8127	JJ	denotes	transcriptional
T3852	8108-8111	DT	denotes	the
T3851	8105-8107	TO	denotes	to
T3850	8096-8104	JJ	denotes	relative
T3849	8092-8095	CD	denotes	+64
T3848	8089-8091	TO	denotes	to
T3847	8085-8088	CD	denotes	208
T3846	8084-8085	VBP	denotes	−
T3845	8072-8083	NNS	denotes	nucleotides
T3844	8062-8071	VBG	denotes	inserting
T3843	8059-8061	IN	denotes	by
T3842	8047-8058	VBN	denotes	constructed
T3841	8042-8046	VBD	denotes	were
T3840	8033-8041	NNS	denotes	plasmids
T3839	8024-8032	NN	denotes	reporter
T3838	8020-8023	NNP	denotes	LTR
T3837	8014-8019	NN	denotes	assay
T3836	8005-8013	NN	denotes	reporter
T3835	8001-8004	CC	denotes	and
T3834	7988-8000	NN	denotes	construction
T3833	7980-7987	JJ	denotes	plasmid
T3832	7976-7979	NNP	denotes	LTR
T7201	16245-16246	NNP	denotes	E
T7200	16237-16244	JJ	denotes	subtype
T7199	16232-16236	IN	denotes	from
T7198	16227-16231	NNS	denotes	LTRs
T7197	16223-16226	DT	denotes	the
T7196	16217-16222	IN	denotes	while
T7195	16215-16216	,	denotes	,
T7194	16207-16215	NN	denotes	activity
T7193	16197-16206	JJS	denotes	strongest
T7192	16193-16196	DT	denotes	the
T7191	16183-16192	VBD	denotes	displayed
T7190	16178-16182	NNPS	denotes	LTRs
T7189	16176-16177	NNP	denotes	C
T7188	16168-16175	NNP	denotes	Subtype
T7187	16166-16167	.	denotes	.
T7186	16150-16166	JJ	denotes	subtype-specific
T7185	16146-16149	VBD	denotes	was
T7184	16139-16145	NN	denotes	effect
T7183	16134-16138	DT	denotes	this
T7182	16131-16133	IN	denotes	of
T7181	16121-16130	NN	denotes	magnitude
T7180	16117-16120	DT	denotes	the
T7179	16113-16116	CC	denotes	and
T7178	16111-16112	-RRB-	denotes	)
T7177	16109-16111	CD	denotes	1A
T7176	16102-16108	NN	denotes	Figure
T7175	16101-16102	-LRB-	denotes	(
T7174	16092-16100	NN	denotes	activity
T7173	16081-16091	NN	denotes	luciferase
T7172	16078-16080	IN	denotes	in
T7171	16066-16077	NN	denotes	enhancement
T7170	16054-16065	JJ	denotes	significant
T7169	16052-16053	DT	denotes	a
T7168	16039-16051	VBD	denotes	demonstrated
T7167	16029-16038	NNS	denotes	reporters
T7166	16018-16028	NNP	denotes	LTR-driven
T7165	16016-16017	NNP	denotes	E
T7164	16012-16015	CC	denotes	and
T7163	16010-16011	,	denotes	,
T7162	16009-16010	NNP	denotes	C
T7161	16007-16008	,	denotes	,
T7160	16006-16007	NNP	denotes	B
T7159	16002-16005	DT	denotes	the
T7158	16000-16001	,	denotes	,
T7157	15989-16000	NN	denotes	stimulation
T7156	15984-15988	IN	denotes	Upon
T7155	15982-15983	.	denotes	.
T7154	15981-15982	-RRB-	denotes	)
T7234	16425-16426	.	denotes	.
T7233	16424-16425	NNP	denotes	]
T7232	16422-16424	CD	denotes	39
T7231	16421-16422	NNP	denotes	[
T7230	16419-16420	,	denotes	,
T7229	16418-16419	NNP	denotes	]
T7228	16416-16418	CD	denotes	38
T7227	16415-16416	NNP	denotes	[
T7226	16406-16414	NN	denotes	stimulus
T7225	16404-16405	DT	denotes	a
T7224	16401-16403	IN	denotes	as
T7223	16397-16400	NNP	denotes	TNF
T7222	16392-16396	VBD	denotes	used
T7221	16387-16391	WDT	denotes	that
T7220	16378-16386	NN	denotes	activity
T7219	16374-16377	NNP	denotes	LTR
T7218	16357-16373	JJ	denotes	subtype-specific
T7217	16343-16356	VBG	denotes	demonstrating
T7216	16335-16342	NNS	denotes	studies
T7215	16326-16334	JJ	denotes	previous
T7214	16321-16325	IN	denotes	with
T7213	16310-16320	JJ	denotes	consistent
T7212	16308-16309	,	denotes	,
T7211	16307-16308	-RRB-	denotes	)
T7210	16305-16307	CD	denotes	1A
T7209	16298-16304	NN	denotes	Figure
T7208	16297-16298	-LRB-	denotes	(
T7207	16288-16296	NN	denotes	activity
T7206	16280-16287	JJS	denotes	weakest
T7205	16276-16279	DT	denotes	the
T7204	16269-16275	VBD	denotes	showed
T7203	16256-16268	RB	denotes	consistently
T7202	16247-16255	VBZ	denotes	isolates
T3207	6417-6418	,	denotes	,
T3206	6410-6417	NNP	denotes	Peapack
T3205	6408-6409	,	denotes	,
T3204	6397-6408	NNP	denotes	Corporation
T3203	6387-6396	NNP	denotes	Pharmacia
T3202	6386-6387	-LRB-	denotes	(
T3201	6371-6385	NNP	denotes	Ficoll-Hypaque
T3200	6368-6370	IN	denotes	by
T3199	6359-6367	VBN	denotes	isolated
T3198	6354-6358	VBD	denotes	were
T3197	6347-6353	NNS	denotes	donors
T3196	6334-6346	JJ	denotes	unidentified
T3195	6327-6333	JJ	denotes	normal
T3194	6322-6326	IN	denotes	from
T3193	6317-6321	NNP	denotes	PBMC
T3192	6309-6316	NN	denotes	culture
T3191	6304-6308	NNP	denotes	Cell
T5368	11377-11379	IN	denotes	in
T5367	11367-11376	VBN	denotes	validated
T569	436-440	IN	denotes	that
T570	441-450	VBG	denotes	silencing
T571	451-453	IN	denotes	of
T572	454-457	DT	denotes	the
T573	458-468	NN	denotes	expression
T574	469-471	IN	denotes	of
T575	472-475	DT	denotes	the
T576	476-489	NN	denotes	transcription
T577	490-496	NN	denotes	factor
T578	497-504	JJ	denotes	nuclear
T579	505-511	NN	denotes	factor
T580	512-514	IN	denotes	of
T581	515-524	VBN	denotes	activated
T582	525-526	NNP	denotes	T
T583	527-532	NNS	denotes	cells
T584	533-534	CD	denotes	5
T585	535-536	-LRB-	denotes	(
T586	536-541	CD	denotes	NFAT5
T587	541-542	-RRB-	denotes	)
T648	878-881	CC	denotes	and
T649	882-889	JJ	denotes	subtype
T650	890-891	NNP	denotes	C
T651	892-901	JJ	denotes	molecular
T4275	9500-9512	VBG	denotes	footprinting
T652	902-908	NNS	denotes	clones
T653	909-911	VBZ	denotes	is
T654	912-920	VBN	denotes	required
T655	921-924	IN	denotes	for
T656	925-933	JJ	denotes	efficent
T657	934-943	NN	denotes	induction
T658	944-946	IN	denotes	of
T659	947-952	JJ	denotes	HIV-1
T660	953-964	NN	denotes	replication
T661	965-967	IN	denotes	by
T662	968-971	NNP	denotes	MTb
T663	971-972	.	denotes	.
T664	973-984	RB	denotes	Furthermore
T665	984-985	,	denotes	,
T666	986-995	VBG	denotes	silencing
T667	996-998	IN	denotes	by
T668	999-1003	NNS	denotes	RNAi
T669	1004-1006	IN	denotes	of
T670	1007-1010	JJ	denotes	key
T671	1011-1021	NNS	denotes	components
T672	1022-1024	IN	denotes	of
T673	1025-1028	DT	denotes	the
T674	1029-1032	NNP	denotes	TLR
T675	1033-1040	NN	denotes	pathway
T676	1041-1043	IN	denotes	in
T677	1044-1049	JJ	denotes	human
T678	1050-1059	NNS	denotes	monocytes
T679	1059-1060	,	denotes	,
T680	1061-1070	VBG	denotes	including
T681	1071-1074	DT	denotes	the
T682	1075-1085	JJ	denotes	downstream
T4274	9498-9499	PRP	denotes	I
T4284	9555-9556	.	denotes	.
T4283	9554-9555	NNP	denotes	]
T4282	9552-9554	CD	denotes	31
T4281	9551-9552	NNP	denotes	[
T4280	9541-9550	VBN	denotes	described
T4279	9530-9540	RB	denotes	previously
T4278	9527-9529	IN	denotes	as
T4277	9517-9526	VBN	denotes	performed
T4276	9513-9516	VBD	denotes	was
T4273	9492-9497	NNP	denotes	DNase
T4272	9479-9491	JJ	denotes	Quantitative
T4271	9477-9478	.	denotes	.
T4270	9476-9477	-RRB-	denotes	)
T4269	9457-9476	NN	denotes	www.activemotif.com
T4268	9455-9456	,	denotes	,
T4267	9450-9455	NN	denotes	Motif
T4266	9443-9449	JJ	denotes	Active
T4265	9442-9443	-LRB-	denotes	(
T4264	9432-9441	VBN	denotes	purchased
T4263	9427-9431	VBD	denotes	were
T4262	9423-9426	NNS	denotes	p65
T4261	9419-9422	CC	denotes	and
T4260	9415-9418	NN	denotes	p50
T4259	9403-9414	JJ	denotes	Recombinant
T4258	9401-9402	.	denotes	.
T4257	9400-9401	-RRB-	denotes	)
T4256	9394-9400	NNP	denotes	Qiagen
T4255	9393-9394	-LRB-	denotes	(
T4254	9385-9392	NN	denotes	agarose
T4253	9378-9384	JJ	denotes	Ni-NTA
T4252	9372-9377	VBG	denotes	using
T4251	9361-9371	NNS	denotes	conditions
T4250	9354-9360	JJ	denotes	native
T4249	9348-9353	IN	denotes	under
T4248	9339-9347	VBN	denotes	purified
T4247	9335-9338	CC	denotes	and
T4246	9333-9334	-RRB-	denotes	)
T4245	9315-9333	NN	denotes	www.stratagene.com
T4244	9313-9314	:	denotes	;
T4243	9303-9313	NNP	denotes	Stratagene
T4242	9302-9303	-LRB-	denotes	(
T4241	9296-9301	NNS	denotes	cells
T4240	9294-9295	-RRB-	denotes	)
T4239	9291-9294	NNP	denotes	DE3
T4238	9290-9291	-LRB-	denotes	(
R268	T515	T517	compound	Regulation,tuberculosis-Dependent
R269	T516	T517	compound	of Mycobacterium,tuberculosis-Dependent
R270	T517	T520	nsubj	tuberculosis-Dependent,Reveals
R271	T518	T519	compound	HIV-1,Transcription
R272	T519	T520	nsubj	Transcription,Reveals
R273	T520	T520	ROOT	Reveals,Reveals
R274	T521	T523	det	a,Role
R275	T522	T523	compound	New,Role
R276	T523	T520	dobj	Role,Reveals
R277	T524	T523	prep	for,Role
R278	T525	T524	pobj	NFAT5,for
R279	T526	T523	prep	in,Role
R280	T527	T531	det	the,Tuberculosis
R281	T528	T531	compound	Toll-Like,Tuberculosis
R282	T529	T531	compound	Receptor,Tuberculosis
R283	T530	T531	compound	Pathway,Tuberculosis
R284	T531	T526	pobj	Tuberculosis,in
R285	T532	T531	punct	(,Tuberculosis
R286	T533	T531	appos	TB,Tuberculosis
R287	T534	T531	punct	),Tuberculosis
R288	T535	T523	conj	disease,Role
R289	T536	T535	prep	in,disease
R290	T537	T536	pobj	HIV,in
R291	T538	T539	amod	co-infected,patients
R292	T539	T523	conj	patients,Role
R293	T540	T520	conj	contributes,Reveals
R294	T541	T540	prep	to,contributes
R295	T542	T543	amod	increased,mortality
R296	T543	T541	pobj	mortality,to
R297	T544	T540	prep	by,contributes
R298	T545	T544	pcomp	activating,by
R299	T546	T545	dobj	innate,activating
R300	T547	T546	cc	and,innate
R301	T548	T550	amod	adaptive,signaling
R302	T549	T550	amod	immune,signaling
R303	T550	T545	conj	signaling,activating
R304	T551	T550	dobj	cascades,signaling
R305	T552	T553	nsubj	that,stimulate
R306	T553	T551	relcl	stimulate,cascades
R307	T554	T555	compound	HIV-1,replication
R308	T555	T553	dobj	replication,stimulate
R309	T556	T550	punct	",",signaling
R310	T557	T550	conj	leading,signaling
R311	T558	T557	prep	to,leading
R312	T559	T560	det	an,increase
R313	T560	T558	pobj	increase,to
R314	T561	T560	prep	in,increase
R315	T562	T563	amod	viral,load
R316	T563	T561	pobj	load,in
R317	T564	T520	punct	.,Reveals
R318	T565	T568	advmod	Here,demonstrate
R319	T566	T568	punct	",",demonstrate
R320	T567	T568	nsubj	we,demonstrate
R321	T568	T568	ROOT	demonstrate,demonstrate
R322	T588	T570	prep	by,silencing
R323	T589	T590	compound	RNA,interference
R324	T590	T588	pobj	interference,by
R325	T591	T592	punct	(,RNAi
R326	T592	T570	appos	RNAi,silencing
R327	T593	T570	punct	),silencing
R328	T594	T568	ccomp	inhibits,demonstrate
R329	T595	T596	compound	Mycobacterium,tuberculosis
R330	T596	T594	dobj	tuberculosis,inhibits
R331	T597	T596	punct	(,tuberculosis
R332	T598	T596	appos	MTb,tuberculosis
R333	T599	T596	punct	),tuberculosis
R334	T600	T596	punct	-,tuberculosis
R335	T601	T568	advcl	stimulated,demonstrate
R336	T602	T603	compound	HIV-1,replication
R337	T603	T601	dobj	replication,stimulated
R338	T604	T603	prep	in,replication
R339	T605	T606	amod	co-infected,macrophages
R340	T606	T604	pobj	macrophages,in
R341	T607	T568	punct	.,demonstrate
R342	T608	T609	nsubj	We,show
R343	T609	T609	ROOT	show,show
R344	T610	T618	mark	that,induced
R345	T611	T618	nsubjpass	NFAT5,induced
R346	T612	T618	nsubjpass	gene,induced
R347	T613	T612	cc	and,gene
R348	T614	T615	compound	protein,expression
R349	T615	T612	conj	expression,gene
R350	T616	T618	auxpass	are,induced
R351	T617	T618	advmod	strongly,induced
R352	T618	T609	ccomp	induced,show
R353	T619	T618	agent	by,induced
R354	T620	T619	pobj	MTb,by
R355	T621	T620	punct	",",MTb
R356	T622	T623	nsubj	which,is
R357	T623	T620	relcl	is,MTb
R358	T624	T626	det	a,receptor
R359	T625	T626	amod	Toll-like,receptor
R360	T626	T623	attr	receptor,is
R361	T627	T628	punct	(,TLR
R362	T628	T623	npadvmod	TLR,is
R363	T629	T618	punct	),induced
R364	T630	T618	conj	ligand,induced
R365	T631	T630	punct	",",ligand
R366	T632	T618	cc	and,induced
R367	T633	T654	mark	that,required
R368	T634	T638	det	an,site
R369	T635	T638	amod	intact,site
R370	T636	T638	nmod	NFAT5,site
R371	T637	T638	amod	binding,site
R372	T638	T654	nsubjpass	site,required
R373	T639	T638	prep	in,site
R374	T640	T642	det	the,promoter
R375	T641	T642	amod	viral,promoter
R376	T642	T639	pobj	promoter,in
R377	T643	T642	prep	of,promoter
R378	T644	T647	nmod	R5-tropic,B
R379	T645	T647	nmod	HIV-1,B
R380	T646	T647	compound	subtype,B
R381	T647	T652	nmod	B,clones
R382	T683	T684	amod	signaling,molecules
R383	T684	T680	pobj	molecules,including
R384	T685	T680	pobj	MyD88,including
R385	T686	T685	punct	",",MyD88
R386	T687	T685	conj	IRAK1,MyD88
R387	T688	T687	punct	",",IRAK1
R388	T689	T687	cc	and,IRAK1
R389	T690	T687	conj	TRAF6,IRAK1
R390	T691	T693	punct	",",inhibits
R391	T692	T693	advmod	significantly,inhibits
R392	T693	T693	ROOT	inhibits,inhibits
R393	T694	T697	amod	MTb-induced,expression
R394	T695	T697	nummod	NFAT5,expression
R395	T696	T697	compound	gene,expression
R396	T697	T693	dobj	expression,inhibits
R397	T698	T693	punct	.,inhibits
R398	T699	T708	advmod	Thus,induces
R399	T700	T708	punct	",",induces
R400	T701	T704	det	the,response
R401	T702	T704	amod	innate,response
R402	T703	T704	amod	immune,response
R403	T704	T708	nsubj	response,induces
R404	T705	T704	prep	to,response
R405	T706	T707	compound	MTb,infection
R406	T707	T705	pobj	infection,to
R407	T708	T708	ROOT	induces,induces
R408	T709	T710	compound	NFAT5,gene
R409	T710	T708	dobj	gene,induces
R410	T711	T710	cc	and,gene
R411	T712	T713	compound	protein,expression
R412	T713	T710	conj	expression,gene
R413	T714	T708	punct	",",induces
R414	T715	T708	cc	and,induces
R415	T716	T717	nsubj	NFAT5,plays
R416	T717	T708	conj	plays,induces
R417	T718	T720	det	a,role
R418	T719	T720	amod	crucial,role
R419	T720	T717	dobj	role,plays
R420	T721	T720	prep	in,role
R421	T722	T723	compound	MTb,regulation
R422	T723	T721	pobj	regulation,in
R423	T724	T723	prep	of,regulation
R424	T725	T726	compound	HIV-1,replication
R425	T726	T724	pobj	replication,of
R426	T727	T717	prep	via,plays
R427	T728	T730	det	a,interaction
R428	T729	T730	amod	direct,interaction
R429	T730	T727	pobj	interaction,via
R430	T731	T730	prep	with,interaction
R431	T732	T734	det	the,promoter
R432	T733	T734	amod	viral,promoter
R433	T734	T731	pobj	promoter,with
R434	T735	T717	punct	.,plays
R435	T736	T737	det	These,findings
R436	T737	T739	nsubj	findings,demonstrate
R437	T738	T739	advmod	also,demonstrate
R438	T739	T739	ROOT	demonstrate,demonstrate
R439	T740	T742	det	a,role
R440	T741	T742	amod	general,role
R441	T742	T739	dobj	role,demonstrate
R442	T743	T739	prep	for,demonstrate
R443	T744	T743	pobj	NFAT5,for
R444	T745	T744	prep	in,NFAT5
R445	T746	T745	pobj	TLR,in
R446	T747	T744	punct	-,NFAT5
R447	T748	T744	cc	and,NFAT5
R448	T749	T750	amod	MTb-mediated,control
R449	T750	T744	conj	control,NFAT5
R450	T751	T750	prep	of,control
R451	T752	T753	compound	gene,expression
R452	T753	T751	pobj	expression,of
R453	T754	T739	punct	.,demonstrate
R527	T569	T594	mark	that,inhibits
R528	T570	T594	csubj	silencing,inhibits
R529	T571	T570	prep	of,silencing
R530	T572	T573	det	the,expression
R531	T573	T571	pobj	expression,of
R532	T574	T573	prep	of,expression
R533	T575	T577	det	the,factor
R534	T576	T577	compound	transcription,factor
R535	T577	T574	pobj	factor,of
R536	T578	T579	amod	nuclear,factor
R537	T579	T573	conj	factor,expression
R538	T580	T573	prep	of,expression
R539	T581	T583	amod	activated,cells
R540	T582	T583	compound	T,cells
R541	T583	T580	pobj	cells,of
R542	T584	T570	nummod	5,silencing
R543	T585	T586	punct	(,NFAT5
R544	T586	T584	prep	NFAT5,5
R545	T587	T584	punct	),5
R546	T648	T647	cc	and,B
R547	T649	T652	amod	subtype,clones
R548	T650	T652	nmod	C,clones
R549	T651	T652	amod	molecular,clones
R550	T652	T643	pobj	clones,of
R551	T653	T654	auxpass	is,required
R552	T654	T618	conj	required,induced
R553	T655	T654	prep	for,required
R554	T656	T657	amod	efficent,induction
R555	T657	T655	pobj	induction,for
R556	T658	T657	prep	of,induction
R557	T659	T660	compound	HIV-1,replication
R558	T660	T658	pobj	replication,of
R559	T661	T657	prep	by,induction
R560	T662	T661	pobj	MTb,by
R561	T663	T609	punct	.,show
R562	T664	T693	advmod	Furthermore,inhibits
R563	T665	T693	punct	",",inhibits
R564	T666	T693	advcl	silencing,inhibits
R565	T667	T666	prep	by,silencing
R566	T668	T667	pobj	RNAi,by
R567	T669	T668	prep	of,RNAi
R568	T670	T671	amod	key,components
R569	T671	T669	pobj	components,of
R570	T672	T671	prep	of,components
R571	T673	T675	det	the,pathway
R572	T674	T675	compound	TLR,pathway
R573	T675	T672	pobj	pathway,of
R574	T676	T675	prep	in,pathway
R575	T677	T678	amod	human,monocytes
R576	T678	T676	pobj	monocytes,in
R577	T679	T678	punct	",",monocytes
R578	T680	T678	prep	including,monocytes
R579	T681	T684	det	the,molecules
R580	T682	T684	amod	downstream,molecules
R1404	T1927	T1928	compound	Mycobacterium,tuberculosis
R1405	T1928	T1942	nsubj	tuberculosis,is
R1407	T1929	T1930	punct	(,MTb
R1411	T1930	T1928	appos	MTb,tuberculosis
R1413	T1931	T1930	punct	),MTb
R1452	T2010	T2009	acl	produced,chemokines
R1454	T2011	T2010	agent	by,produced
R1455	T2012	T2014	det	the,host
R1457	T2013	T2014	amod	human,host
R1458	T2014	T2011	pobj	host,by
R1459	T2015	T2010	prep	in,produced
R1461	T2016	T2015	pobj	response,in
R1462	T2017	T2016	prep	to,response
R1463	T2018	T2020	dep	MTb,activate
R1465	T2019	T2020	compound	infection,activate
R1466	T2020	T2020	ROOT	activate,activate
R1467	T2021	T2023	nmod	signal,pathways
R1468	T2022	T2023	compound	transduction,pathways
R1469	T2023	T2020	dobj	pathways,activate
R1470	T2024	T2023	prep	in,pathways
R1472	T2025	T2026	compound	CD4,T
R1473	T2026	T2027	compound	T,cells
R1474	T2027	T2024	pobj	cells,in
R1476	T2028	T2027	cc	and,cells
R1477	T2029	T2030	amod	monocytic,cells
R1478	T2030	T2027	conj	cells,cells
R1480	T2031	T2033	nsubj	that,result
R1481	T2032	T2033	advmod	also,result
R1482	T2033	T2023	relcl	result,pathways
R1483	T2034	T2033	prep	in,result
R1485	T2035	T2036	amod	transcriptional,activation
R1486	T2036	T2034	pobj	activation,in
R1488	T2037	T2036	prep	of,activation
R1489	T2038	T2046	det	the,]
R1490	T2039	T2041	compound	HIV-1,[
R1491	T2040	T2041	compound	LTR,[
R1493	T2041	T2046	compound	[,]
R1494	T2042	T2043	nummod	4,]
R1495	T2043	T2044	nmod	],[
R1497	T2044	T2046	nmod	[,]
R1498	T2045	T2044	nummod	6,[
R1499	T2046	T2037	pobj	],of
R1501	T2047	T2020	punct	.,activate
R1502	T2048	T2057	nsubj	Activation,leads
R1503	T2049	T2048	prep	of,Activation
R1505	T2050	T2049	pobj	HIV-1,of
R1506	T2051	T2048	conj	replication,Activation
R1507	T2052	T2048	prep	via,Activation
R1509	T2053	T2055	det	these,pathways
R1511	T2054	T2055	amod	MTb-induced,pathways
R1512	T2055	T2052	pobj	pathways,via
R1513	T2056	T2057	advmod	ultimately,leads
R1514	T2057	T2057	ROOT	leads,leads
R1516	T2058	T2057	prep	to,leads
R1517	T2059	T2061	amod	higher,loads
R1518	T2060	T2061	amod	viral,loads
R1519	T2061	T2058	pobj	loads,to
R1520	T2062	T2061	cc	and,loads
R1521	T2063	T2081	punct	",",reviewed
R1523	T2064	T2081	prep	in,reviewed
R1524	T2065	T2064	pobj	turn,in
R1525	T2066	T2081	punct	",",reviewed
R1526	T2067	T2071	amod	expedited,loss
R1528	T2068	T2069	compound	CD4,T
R1529	T2069	T2071	compound	T,loss
R1530	T2070	T2071	compound	cell,loss
R1532	T2071	T2081	nsubj	loss,reviewed
R1533	T2072	T2071	cc	and,loss
R1535	T2073	T2071	conj	progression,loss
R1536	T2074	T2071	prep	to,loss
R1537	T2075	T2074	pobj	AIDS,to
R1538	T2076	T2075	punct	(,AIDS
R1540	T2077	T2075	appos	[,AIDS
R1541	T2078	T2079	nummod	7,]
R1543	T2079	T2075	conj	],AIDS
R1544	T2080	T2071	punct	",",loss
R1545	T2081	T2057	advcl	reviewed,leads
R1547	T2082	T2081	prep	in,reviewed
R1548	T2083	T2085	amod	[,]
R1549	T2084	T2085	nummod	8,]
R1551	T2085	T2082	pobj	],in
R1552	T2086	T2085	appos	[,]
R1553	T2087	T2088	nummod	10,]
R1555	T2088	T2085	appos	],]
R1557	T1978	T1976	appos	PRRs,receptors
R1559	T2090	T2057	punct	.,leads
R1560	T2091	T2102	advmod	Furthermore,is
R1561	T1979	T1976	punct	),receptors
R1562	T1980	T1972	prep	by,engagement
R1563	T1981	T1980	pobj	MTb,by
R1564	T1982	T1972	prep	on,engagement
R1565	T1983	T1984	compound	mononuclear,phagocytes
R1566	T2092	T2102	punct	",",is
R1567	T2093	T2096	det	the,compromise
R1568	T2094	T2096	amod	progressive,compromise
R1569	T2095	T2096	amod	immune,compromise
R1570	T2096	T2102	nsubj	compromise,is
R1571	T1984	T1982	pobj	phagocytes,on
R1572	T2097	T2096	acl	associated,compromise
R1573	T2098	T2097	prep	with,associated
R1574	T1985	T1985	ROOT	activates,activates
R1575	T2099	T2100	compound	HIV-1,infection
R1576	T2100	T2098	pobj	infection,with
R1577	T1986	T1985	xcomp	signaling,activates
R1578	T2101	T2100	appos	itself,infection
R1579	T2102	T2102	ROOT	is,is
R1580	T2103	T2105	det	a,cause
R1581	T1987	T1986	dobj	cascades,signaling
R1582	T2104	T2105	amod	major,cause
R1583	T2105	T2102	attr	cause,is
R1584	T2106	T2105	prep	of,cause
R1585	T1988	T1990	nsubj	that,induce
R1586	T2107	T2109	amod	latent,reactivation
R1587	T2108	T2109	compound	MTb,reactivation
R1588	T2109	T2106	pobj	reactivation,of
R1589	T1989	T1990	advmod	directly,induce
R1590	T2110	T2105	punct	",",cause
R1591	T2111	T2113	advmod	as,as
R1592	T2112	T2113	advmod	well,as
R1593	T2113	T2105	cc	as,cause
R1594	T1990	T1987	relcl	induce,cascades
R1595	T2114	T2115	amod	increased,susceptibility
R1596	T2115	T2105	conj	susceptibility,cause
R1597	T2116	T2115	prep	to,susceptibility
R1598	T1991	T1990	dobj	transcription,induce
R1599	T2117	T2119	amod	primary,infection
R1600	T2118	T2119	compound	TB,infection
R1601	T1992	T1990	prep	from,induce
R1602	T2119	T2116	pobj	infection,to
R1603	T2120	T2116	punct	(,to
R1604	T2121	T2128	nsubj	[,reviewed
R1605	T1993	T1995	det	the,LTR
R1606	T2122	T2123	compound	11,]
R1607	T2123	T2121	dobj	],[
R1608	T2124	T2126	nmod	[,]
R1609	T2125	T2126	nummod	15,]
R1610	T1994	T1995	compound	proviral,LTR
R1611	T2126	T2121	conj	],[
R1612	T2127	T2126	punct	",",]
R1613	T2189	T2187	appos	[,TRAF6
R1614	T2128	T2102	advcl	reviewed,is
R1615	T2129	T2128	prep	in,reviewed
R1616	T2130	T2132	compound	[,]
R1617	T2131	T2132	nummod	8,]
R1618	T2132	T2129	pobj	],in
R1619	T2133	T2128	punct	),reviewed
R1620	T2134	T2102	punct	.,is
R1621	T2190	T2191	nummod	21,]
R1622	T2135	T2137	det	The,PRR
R1623	T2136	T2137	amod	primary,PRR
R1624	T2137	T2145	nsubj	PRR,is
R1625	T2138	T2137	prep	on,PRR
R1626	T2191	T2187	appos	],TRAF6
R1627	T2139	T2140	amod	monocytic,cells
R1628	T2140	T2138	pobj	cells,on
R1629	T2192	T2187	appos	[,TRAF6
R1630	T2141	T2140	acl	triggered,cells
R1631	T2142	T2141	agent	by,triggered
R1632	T2143	T2144	compound	MTb,infection
R1633	T2193	T2194	nummod	23,]
R1634	T2144	T2142	pobj	infection,by
R1635	T2145	T2145	ROOT	is,is
R1636	T2146	T2147	amod	toll-like,receptor
R1637	T2194	T2187	appos	],TRAF6
R1638	T2147	T2145	attr	receptor,is
R1639	T2148	T2149	punct	(,TLR
R1640	T2149	T2147	appos	TLR,receptor
R1641	T2195	T2196	punct	",",reviewed
R1642	T2150	T2147	punct	),receptor
R1643	T2151	T2154	nummod	2,]
R1644	T2196	T2196	ROOT	reviewed,reviewed
R1645	T2152	T2154	nmod	[,]
R1646	T2153	T2154	nummod	16,]
R1647	T2154	T2155	nmod	],[
R1648	T2155	T2157	nmod	[,]
R1649	T2197	T2196	prep	in,reviewed
R1650	T2156	T2157	nummod	20,]
R1651	T2157	T2145	npadvmod	],is
R1652	T2158	T2145	punct	.,is
R1653	T2198	T2197	pobj	[,in
R1654	T2159	T2196	nsubj	Engagement,reviewed
R1655	T2160	T2159	prep	of,Engagement
R1656	T2161	T2162	nummod	TLR2,results
R1657	T2199	T2200	nummod	10,]
R1658	T2162	T2160	pobj	results,of
R1659	T2163	T2162	prep	in,results
R1660	T2164	T2163	pobj	engagement,in
R1661	T2200	T2196	npadvmod	],reviewed
R1662	T2165	T2164	prep	of,engagement
R1663	T2201	T2200	punct	",",]
R1664	T2166	T2168	det	the,protein
R1665	T2202	T2200	conj	[,]
R1666	T2167	T2168	compound	adaptor,protein
R1667	T2168	T2165	pobj	protein,of
R1668	T2169	T2168	appos	MyD88,protein
R1669	T2170	T2164	cc	and,engagement
R1670	T2203	T2204	nummod	24,]
R1671	T2171	T2173	det	the,recruitment
R1672	T2172	T2173	amod	subsequent,recruitment
R1673	T2173	T2164	conj	recruitment,engagement
R1674	T2174	T2173	prep	of,recruitment
R1675	T2204	T2204	ROOT	],]
R1676	T2175	T2176	amod	several,kinases
R1677	T2176	T2174	pobj	kinases,of
R1678	T2205	T2202	punct	),[
R1679	T2177	T2176	punct	",",kinases
R1680	T2178	T2176	prep	including,kinases
R1681	T2206	T2196	punct	.,reviewed
R1682	T2179	T2178	pobj	IRAK1,including
R1683	T2180	T2179	cc	and,IRAK1
R1684	T2181	T2179	conj	IRAK4,IRAK1
R1685	T2207	T2208	nsubj	TRAF6,activates
R1686	T2182	T2181	punct	",",IRAK4
R1687	T2183	T2181	cc	and,IRAK4
R1688	T2184	T2196	det	the,reviewed
R1689	T2208	T2208	ROOT	activates,activates
R1690	T2185	T2196	npadvmod	ubiquitin,reviewed
R1691	T2186	T2187	compound	ligase,TRAF6
R1692	T2187	T2185	appos	TRAF6,ubiquitin
R1693	T2188	T2189	punct	(,[
R1694	T2209	T2210	compound	IκB,kinase
R1695	T2210	T2208	dobj	kinase,activates
R1696	T2211	T2212	punct	(,IKK
R1697	T2212	T2210	appos	IKK,kinase
R1698	T2213	T2212	punct	),IKK
R1699	T2214	T2210	cc	and,kinase
R1700	T2286	T2285	prep	of,LTR
R1701	T2287	T2288	det	each,subtype
R1702	T2215	T2216	amod	mitogen-activated,protein
R1703	T2288	T2286	pobj	subtype,of
R1704	T2289	T2279	conj	is,comprises
R1705	T2216	T2210	conj	protein,kinase
R1706	T2290	T2289	acomp	unique,is
R1707	T2291	T2290	prep	with,unique
R1708	T2292	T2291	pobj	respect,with
R1709	T2217	T2218	punct	(,MAP
R1710	T2293	T2292	prep	to,respect
R1711	T2294	T2295	det	the,number
R1712	T2295	T2293	pobj	number,to
R1713	T2218	T2216	appos	MAP,protein
R1714	T2296	T2295	cc	and,number
R1715	T2297	T2295	conj	organization,number
R1716	T2298	T2295	prep	of,number
R1717	T2219	T2216	punct	),protein
R1718	T2299	T2301	amod	activator,sites
R1719	T2300	T2301	amod	binding,sites
R1720	T2301	T2298	pobj	sites,of
R1721	T2302	T2289	punct	.,is
R1722	T2303	T2333	prep	For,has
R1723	T2220	T2208	conj	kinases,activates
R1724	T2304	T2303	pobj	example,For
R1725	T2305	T2333	punct	",",has
R1726	T2221	T2227	mark	that,induce
R1727	T2306	T2308	compound	HIV-1,B
R1728	T2307	T2308	compound	subtype,B
R1729	T2308	T2333	nsubj	B,has
R1730	T2222	T2227	punct	",",induce
R1731	T2309	T2308	punct	",",B
R1732	T2310	T2315	det	the,subtype
R1733	T2311	T2313	advmod	most,characterized
R1734	T2223	T2227	prep	in,induce
R1735	T2312	T2313	advmod	highly,characterized
R1736	T2313	T2315	amod	characterized,subtype
R1737	T2314	T2315	amod	viral,subtype
R1738	T2224	T2223	pobj	turn,in
R1739	T2315	T2308	appos	subtype,B
R1740	T2316	T2315	cc	and,subtype
R1741	T2317	T2319	det	the,cause
R1742	T2318	T2319	amod	primary,cause
R1743	T2225	T2227	punct	",",induce
R1744	T2319	T2315	conj	cause,subtype
R1745	T2320	T2319	prep	of,cause
R1746	T2321	T2320	pobj	infection,of
R1747	T2226	T2227	advmod	ultimately,induce
R1748	T2322	T2321	prep	in,infection
R1749	T2227	T2220	ccomp	induce,kinases
R1750	T2323	T2324	det	the,Americas
R1751	T2228	T2227	dobj	activation,induce
R1752	T2324	T2322	pobj	Americas,in
R1753	T2325	T2324	punct	",",Americas
R1754	T2229	T2228	prep	of,activation
R1755	T2326	T2324	conj	Europe,Americas
R1756	T2327	T2326	punct	",",Europe
R1757	T2328	T2326	conj	Japan,Europe
R1758	T2230	T2233	amod	specific,families
R1759	T2329	T2328	punct	",",Japan
R1760	T2330	T2328	cc	and,Japan
R1761	T2331	T2328	conj	Australia,Japan
R1762	T2231	T2232	compound	transcription,factor
R1763	T2332	T2333	punct	",",has
R1764	T2333	T2333	ROOT	has,has
R1765	T2232	T2233	compound	factor,families
R1766	T2334	T2337	nummod	two,motifs
R1767	T2335	T2337	nmod	tandem,motifs
R1768	T2336	T2337	compound	NF-κB,motifs
R1769	T2337	T2333	dobj	motifs,has
R1770	T2338	T2333	prep	in,has
R1771	T2233	T2229	pobj	families,of
R1772	T2339	T2340	poss	its,LTR
R1773	T2340	T2338	pobj	LTR,in
R1774	T2341	T2333	punct	.,has
R1775	T2234	T2233	punct	",",families
R1776	T2342	T2346	prep	By,subtypes
R1777	T2343	T2342	pobj	contrast,By
R1778	T2344	T2346	punct	",",subtypes
R1779	T2235	T2233	prep	including,families
R1780	T2345	T2346	nsubj	HIV-1,subtypes
R1781	T2346	T2346	ROOT	subtypes,subtypes
R1782	T2347	T2346	dobj	C,subtypes
R1783	T2236	T2237	det	the,NF-κB
R1784	T2348	T2347	cc	and,C
R1785	T2349	T2347	conj	E,C
R1786	T2237	T2235	pobj	NF-κB,including
R1787	T2350	T2347	punct	",",C
R1788	T2351	T2353	nsubj	which,spread
R1789	T2352	T2353	aux	have,spread
R1790	T2238	T2237	cc	and,NF-κB
R1791	T2353	T2347	relcl	spread,C
R1792	T2354	T2353	advmod	disproportionately,spread
R1793	T2355	T2353	prep	in,spread
R1794	T2239	T2240	amod	AP-1,families
R1795	T2356	T2358	amod	TB-burdened,Africa
R1796	T2357	T2358	compound	sub-Saharan,Africa
R1797	T2358	T2355	pobj	Africa,in
R1798	T2359	T2358	cc	and,Africa
R1799	T2240	T2237	conj	families,NF-κB
R1800	T2360	T2361	amod	southeast,Asia
R1801	T2361	T2358	conj	Asia,Africa
R1802	T2362	T2363	punct	",",have
R1803	T2241	T2237	punct	",",NF-κB
R1804	T2363	T2346	conj	have,subtypes
R1805	T2364	T2369	nummod	three,sites
R1806	T2365	T2364	cc	and,three
R1807	T2242	T2245	nsubjpass	which,shown
R1808	T2366	T2364	conj	one,three
R1809	T2243	T2245	aux	have,shown
R1810	T2244	T2245	auxpass	been,shown
R1811	T2367	T2369	amod	NF-κB,sites
R1812	T2245	T2237	relcl	shown,NF-κB
R1813	T2368	T2369	amod	binding,sites
R1814	T2369	T2363	dobj	sites,have
R1815	T2370	T2369	punct	",",sites
R1816	T2246	T2247	aux	to,associate
R1817	T2371	T2374	advmod	respectively,]
R1818	T2372	T2374	nmod	[,]
R1819	T2373	T2374	nummod	1,]
R1820	T2374	T2381	nmod	],]
R1821	T2375	T2374	punct	",",]
R1822	T2247	T2245	xcomp	associate,shown
R1823	T2376	T2378	compound	[,]
R1824	T2377	T2378	compound	28,]
R1825	T2378	T2374	appos	],]
R1826	T2379	T2374	appos	[,]
R1827	T2248	T2247	prep	with,associate
R1828	T2380	T2381	nummod	30,]
R1829	T2249	T2251	det	the,LTR
R1830	T2381	T2363	npadvmod	],have
R1831	T2382	T2363	punct	.,have
R1832	T2250	T2251	compound	HIV-1,LTR
R1833	T2251	T2248	pobj	LTR,with
R1834	T2252	T2247	cc	and,associate
R1835	T2383	T2385	nsubj	We,showed
R1836	T2384	T2385	advmod	previously,showed
R1837	T2385	T2385	ROOT	showed,showed
R1838	T2253	T2254	aux	to,drive
R1839	T2386	T2412	mark	that,binds
R1840	T2387	T2390	det	the,member
R1841	T2388	T2389	advmod	most,primordial
R1842	T2254	T2247	conj	drive,associate
R1843	T2389	T2390	amod	primordial,member
R1844	T2390	T2412	nsubj	member,binds
R1845	T2391	T2390	prep	of,member
R1846	T2255	T2256	poss	its,transcription
R1847	T2392	T2394	det	the,factor
R1848	T2393	T2394	amod	nuclear,factor
R1849	T2394	T2391	pobj	factor,of
R1850	T2256	T2254	dobj	transcription,drive
R1851	T2395	T2394	prep	of,factor
R1852	T2396	T2398	amod	activated,cells
R1853	T2397	T2398	compound	T,cells
R1854	T2257	T2256	punct	(,transcription
R1855	T2398	T2395	pobj	cells,of
R1856	T2399	T2400	punct	(,NFAT
R1857	T2400	T2398	appos	NFAT,cells
R1858	T2258	T2260	nmod	[,]
R1859	T2259	T2260	nummod	22,]
R1861	T2260	T2269	nsubj	],reviewed
R1866	T2261	T2260	punct	",",]
R1872	T2262	T2264	nmod	[,]
R1876	T2263	T2264	nummod	25,]
R1880	T2264	T2260	conj	],]
R1882	T2265	T2267	nmod	[,]
R1886	T2266	T2267	nummod	27,]
R1891	T2267	T2260	conj	],]
R1895	T2268	T2267	punct	",",]
R1899	T2269	T2256	parataxis	reviewed,transcription
R1900	T2270	T2269	prep	in,reviewed
R1902	T2271	T2270	pobj	[,in
R1906	T2272	T2273	nummod	10,]
R1910	T2273	T2271	appos	],[
R1914	T2274	T2271	punct	),[
R1917	T2275	T2208	punct	.,activates
R1925	T2276	T2279	advmod	Notably,comprises
R1929	T2277	T2279	punct	",",comprises
R1933	T2278	T2279	nsubj	HIV-1,comprises
R1936	T2458	T2461	amod	representative,B
R1937	T2279	T2279	ROOT	comprises,comprises
R1938	T2459	T2461	compound	HIV-1,B
R1939	T2460	T2461	compound	subtype,B
R1940	T2461	T2457	pobj	B,of
R1941	T2280	T2281	amod	several,subtypes
R1942	T2462	T2461	punct	",",B
R1943	T2463	T2461	conj	C,B
R1944	T2464	T2453	punct	",",required
R1945	T2281	T2279	dobj	subtypes,comprises
R1946	T2465	T2453	cc	and,required
R1947	T2282	T2281	punct	",",subtypes
R1948	T2466	T2467	nsubj	E,isolates
R1949	T2467	T2453	conj	isolates,required
R1950	T2283	T2281	cc	and,subtypes
R1951	T2468	T2467	prep	in,isolates
R1952	T2469	T2472	amod	human,macrophages
R1953	T2470	T2472	amod	primary,macrophages
R1954	T2284	T2285	det	the,LTR
R1955	T2471	T2472	amod	monocyte-derived,macrophages
R1956	T2472	T2468	pobj	macrophages,in
R1957	T2473	T2476	punct	(,[
R1958	T2285	T2289	nsubj	LTR,is
R1959	T2474	T2476	nmod	MDM,[
R1960	T2475	T2476	punct	),[
R1961	T2476	T2478	nmod	[,]
R1962	T2480	T2509	prep	Given,speculated
R1963	T2477	T2478	nummod	31,]
R1964	T2478	T2467	dobj	],isolates
R1965	T2481	T2486	mark	that,shown
R1966	T2479	T2467	punct	.,isolates
R1967	T2482	T2486	nsubj	NFAT5,shown
R1968	T2483	T2486	aux	has,shown
R1969	T2484	T2486	advmod	previously,shown
R1970	T2485	T2486	auxpass	been,shown
R1971	T2486	T2480	pcomp	shown,Given
R1972	T2487	T2490	aux	to,activated
R1973	T2488	T2490	auxpass	be,activated
R1974	T2489	T2490	advmod	transcriptionally,activated
R1977	T2490	T2486	xcomp	activated,shown
R1978	T2491	T2490	agent	by,activated
R1980	T2492	T2494	det	the,kinase
R1981	T2493	T2494	compound	MAP,kinase
R1985	T2494	T2491	pobj	kinase,by
R1986	T2495	T2494	appos	p38,kinase
R1987	T2496	T2494	punct	",",kinase
R1989	T2497	T2498	nsubj	which,is
R1992	T2498	T2494	relcl	is,kinase
R1995	T2499	T2498	acomp	downstream,is
R1996	T2500	T2499	prep	of,downstream
R1998	T2501	T2500	pobj	MyD88,of
R2000	T2502	T2498	advcl	signaling,is
R2002	T2503	T2502	punct	",",signaling
R2003	T2504	T2506	nmod	[,]
R2007	T2505	T2506	nummod	32,]
R2009	T2506	T2502	appos	],signaling
R2010	T2507	T2509	punct	",",speculated
R2011	T2508	T2509	nsubj	we,speculated
R2012	T2509	T2509	ROOT	speculated,speculated
R2013	T2560	T2565	mark	that,involved
R2014	T2561	T2565	nsubjpass	NFAT5,involved
R2015	T2562	T2565	aux	may,involved
R2018	T2563	T2564	advmod	also,be
R2020	T2564	T2565	auxpass	be,involved
R2021	T2565	T2509	ccomp	involved,speculated
R2022	T2566	T2565	prep	in,involved
R2023	T2567	T2568	amod	MTb-induced,activation
R2024	T2568	T2566	pobj	activation,in
R2025	T2665	T2663	pobj	replication,of
R2026	T2569	T2568	prep	of,activation
R2027	T2570	T2571	compound	HIV-1,replication
R2028	T2571	T2569	pobj	replication,of
R2029	T2572	T2565	prep	via,involved
R2030	T2666	T2638	punct	.,expand
R2031	T2573	T2575	det	a,pathway
R2032	T2574	T2575	amod	TLR-mediated,pathway
R2033	T2575	T2572	pobj	pathway,via
R2035	T2576	T2575	prep	in,pathway
R2036	T2577	T2576	pobj	monocytes,in
R2037	T2578	T2577	cc	and,monocytes
R2038	T2579	T2582	amod	peripheral,cells
R2039	T2580	T2581	compound	blood,mononuclear
R2040	T2581	T2582	compound	mononuclear,cells
R2041	T2582	T2577	conj	cells,monocytes
R2042	T2583	T2584	punct	(,PBMC
R2044	T2584	T2582	appos	PBMC,cells
R2045	T2585	T2565	punct	),involved
R2046	T2586	T2509	punct	.,speculated
R2048	T2587	T2590	advmod	Here,show
R2049	T2588	T2590	punct	",",show
R2051	T2589	T2590	nsubj	we,show
R2053	T2590	T2590	ROOT	show,show
R2054	T2591	T2598	mark	that,are
R2055	T2592	T2598	nsubj	NFAT5,are
R2056	T2593	T2592	cc	and,NFAT5
R2058	T2594	T2597	poss	its,site
R2059	T2595	T2597	amod	cognate,site
R2060	T2596	T2597	amod	binding,site
R2061	T2597	T2592	conj	site,NFAT5
R2063	T2598	T2590	ccomp	are,show
R2065	T2599	T2598	prep	of,are
R2066	T2600	T2601	amod	crucial,importance
R2067	T2601	T2599	pobj	importance,of
R2068	T2602	T2598	prep	for,are
R2070	T2603	T2605	amod	efficient,stimulation
R2071	T2604	T2605	amod	MTb-induced,stimulation
R2072	T2605	T2602	pobj	stimulation,for
R2073	T2606	T2605	prep	of,stimulation
R2074	T2607	T2608	compound	HIV-1,replication
R2078	T2608	T2606	pobj	replication,of
R2079	T2609	T2605	prep	in,stimulation
R2081	T2610	T2611	amod	human,MDM
R2082	T2611	T2609	pobj	MDM,in
R2083	T2612	T2611	cc	and,MDM
R2085	T2613	T2611	conj	PBMC,MDM
R2086	T2614	T2590	punct	.,show
R2087	T2615	T2618	advmod	Moreover,demonstrate
R2088	T2616	T2618	punct	",",demonstrate
R2089	T2617	T2618	nsubj	we,demonstrate
R2091	T2618	T2618	ROOT	demonstrate,demonstrate
R2092	T2619	T2622	mark	that,increases
R2093	T2620	T2622	nsubj	MTb,increases
R2094	T2621	T2622	nsubj	infection,increases
R2095	T2622	T2618	ccomp	increases,demonstrate
R2097	T2623	T2625	compound	NFAT5,expression
R2098	T2624	T2625	compound	gene,expression
R2099	T2625	T2622	dobj	expression,increases
R2100	T2626	T2625	prep	in,expression
R2101	T2627	T2628	amod	human,monocytes
R2102	T2628	T2626	pobj	monocytes,in
R2103	T2629	T2622	prep	in,increases
R2104	T2630	T2632	det	a,manner
R2105	T2631	T2632	amod	MyD88-dependent,manner
R2106	T2632	T2629	pobj	manner,in
R2108	T2633	T2618	punct	.,demonstrate
R2109	T2634	T2638	advmod	Thus,expand
R2110	T2635	T2638	punct	",",expand
R2111	T2636	T2637	det	these,results
R2112	T2637	T2638	nsubj	results,expand
R2113	T2638	T2638	ROOT	expand,expand
R2114	T2639	T2641	det	the,stimuli
R2115	T2640	T2641	amod	known,stimuli
R2116	T2641	T2638	dobj	stimuli,expand
R2117	T2642	T2641	prep	of,stimuli
R2118	T2643	T2644	nummod	NFAT5,expression
R2119	T2644	T2642	pobj	expression,of
R2121	T2645	T2644	prep	to,expression
R2122	T2646	T2650	det	the,response
R2123	T2647	T2650	amod	PRR-mediated,response
R2124	T2648	T2650	amod	innate,response
R2125	T2649	T2650	amod	immune,response
R2126	T2650	T2645	pobj	response,to
R2127	T2651	T2638	punct	",",expand
R2128	T2652	T2638	cc	and,expand
R2129	T2653	T2638	conj	demonstrate,expand
R2130	T2654	T2656	mark	that,is
R2131	T2655	T2656	nsubj	NFAT5,is
R2132	T2656	T2653	ccomp	is,demonstrate
R2133	T2657	T2659	det	a,modulator
R2135	T2658	T2659	amod	critical,modulator
R2136	T2659	T2656	attr	modulator,is
R2137	T2660	T2659	prep	of,modulator
R2138	T2661	T2662	amod	MTb-induced,enhancement
R2139	T2662	T2660	pobj	enhancement,of
R2140	T2663	T2662	prep	of,enhancement
R2141	T2664	T2665	amod	HIV-1,replication
R2194	T2848	T2849	compound	Ethics,statement
R2195	T2849	T2849	ROOT	statement,statement
R2196	T2850	T2854	prep	In,used
R2197	T2851	T2852	poss	our,studies
R2198	T2852	T2850	pobj	studies,In
R2199	T2853	T2854	nsubj	we,used
R2200	T2854	T2854	ROOT	used,used
R2201	T2855	T2859	amod	unidentified,cells
R2202	T2856	T2857	amod	human,discarded
R2203	T2857	T2859	amod	discarded,cells
R2204	T2858	T2859	compound	blood,cells
R2205	T2859	T2854	dobj	cells,used
R2206	T2860	T2859	punct	(,cells
R2207	T2861	T2862	amod	peripheral,blood
R2208	T2862	T2864	compound	blood,cells
R2209	T2863	T2864	compound	mononuclear,cells
R2210	T2864	T2859	appos	cells,cells
R2211	T2865	T2859	punct	",",cells
R2212	T2866	T2859	appos	PBMC,cells
R2213	T2867	T2866	punct	),PBMC
R2214	T2868	T2859	punct	",",cells
R2215	T2869	T2871	dobj	which,obtained
R2216	T2870	T2871	nsubj	we,obtained
R2217	T2871	T2859	relcl	obtained,cells
R2218	T2872	T2871	prep	from,obtained
R2219	T2873	T2875	det	the,Bank
R2220	T2874	T2875	compound	Blood,Bank
R2221	T2875	T2872	pobj	Bank,from
R2222	T2876	T2875	prep	of,Bank
R2223	T2877	T2879	poss	Children,Hospital
R2224	T2878	T2877	case	's,Children
R2225	T2879	T2876	pobj	Hospital,of
R2226	T2880	T2879	prep	in,Hospital
R2227	T2881	T2880	pobj	Boston,in
R2228	T2882	T2854	punct	.,used
R2475	T3191	T3192	compound	Cell,culture
R2476	T3192	T3193	compound	culture,PBMC
R2477	T3193	T3199	nsubjpass	PBMC,isolated
R2478	T3194	T3193	prep	from,PBMC
R2479	T3195	T3197	amod	normal,donors
R2480	T3196	T3197	amod	unidentified,donors
R2481	T3197	T3194	pobj	donors,from
R2482	T3198	T3199	auxpass	were,isolated
R2483	T3199	T3199	ROOT	isolated,isolated
R2484	T3200	T3199	agent	by,isolated
R2485	T3201	T3204	nmod	Ficoll-Hypaque,Corporation
R2486	T3202	T3204	punct	(,Corporation
R2487	T3203	T3204	compound	Pharmacia,Corporation
R2488	T3204	T3200	pobj	Corporation,by
R2489	T3205	T3204	punct	",",Corporation
R2490	T3206	T3204	conj	Peapack,Corporation
R2491	T3207	T3206	punct	",",Peapack
R2492	T3208	T3206	conj	NJ,Peapack
R2493	T3209	T3208	punct	),NJ
R2494	T3210	T3212	compound	density,centrifugation
R2495	T3211	T3212	compound	gradient,centrifugation
R2496	T3212	T3199	npadvmod	centrifugation,isolated
R2497	T3213	T3199	cc	and,isolated
R2498	T3214	T3199	conj	were,isolated
R2499	T3215	T3214	acomp	cultured,were
R2500	T3216	T3215	prep	in,cultured
R2501	T3217	T3219	compound	RPMI,medium
R2502	T3218	T3219	compound	1640,medium
R2503	T3219	T3216	pobj	medium,in
R2504	T3220	T3215	prep	with,cultured
R2505	T3221	T3223	nummod	2,L-glutamine
R2506	T3222	T3223	compound	mM,L-glutamine
R2507	T3223	T3227	nmod	L-glutamine,Inc.
R2508	T3224	T3227	punct	(,Inc.
R2509	T3225	T3227	nmod	BioWhittaker,Inc.
R2510	T3226	T3227	punct	",",Inc.
R2511	T3227	T3220	pobj	Inc.,with
R2512	T3228	T3227	punct	",",Inc.
R2513	T3229	T3227	conj	Walkersville,Inc.
R2514	T3230	T3229	punct	",",Walkersville
R2515	T3231	T3229	appos	MD,Walkersville
R2516	T3232	T3215	punct	),cultured
R2517	T3233	T3214	conj	supplemented,were
R2518	T3234	T3233	prep	with,supplemented
R2519	T3235	T3236	nummod	10,%
R2520	T3236	T3240	nmod	%,serum
R2521	T3237	T3240	amod	heat-inactivated,serum
R2522	T3238	T3239	amod	fetal,calf
R2523	T3239	T3240	compound	calf,serum
R2524	T3240	T3234	pobj	serum,with
R2525	T3241	T3240	punct	(,serum
R2526	T3242	T3240	appos	FCS,serum
R2527	T3243	T3240	punct	),serum
R2528	T3244	T3246	punct	(,Bio-Products
R2529	T3245	T3246	compound	Gemini,Bio-Products
R2530	T3246	T3240	appos	Bio-Products,serum
R2531	T3247	T3246	punct	",",Bio-Products
R2532	T3248	T3246	appos	www.gembio.com,Bio-Products
R2533	T3249	T3246	punct	),Bio-Products
R2534	T3250	T3199	punct	.,isolated
R2535	T3251	T3252	compound	Human,monocytes
R2536	T3252	T3253	nsubj	monocytes,were
R2537	T3253	T3253	ROOT	were,were
R2538	T3254	T3253	acomp	isolated,were
R2539	T3255	T3254	prep	from,isolated
R2540	T3256	T3257	compound	PBMC,preparations
R2541	T3257	T3255	pobj	preparations,from
R2542	T3258	T3254	prep	by,isolated
R2543	T3259	T3260	amod	positive,selection
R2544	T3260	T3258	pobj	selection,by
R2545	T3261	T3254	prep	with,isolated
R2546	T3262	T3263	nummod	CD14,microbeads
R2547	T3263	T3261	pobj	microbeads,with
R2548	T3264	T3263	prep	from,microbeads
R2549	T3265	T3266	compound	Miltenyi,Biotec
R2550	T3266	T3264	pobj	Biotec,from
R2551	T3267	T3268	punct	(,www.miltenyibiotec.com
R2552	T3268	T3266	appos	www.miltenyibiotec.com,Biotec
R2553	T3269	T3266	punct	),Biotec
R2554	T3270	T3271	mark	as,described
R2555	T3271	T3254	advcl	described,isolated
R2556	T3272	T3271	agent	by,described
R2557	T3273	T3274	det	the,manufacturer
R2558	T3274	T3272	pobj	manufacturer,by
R2559	T3275	T3254	punct	",",isolated
R2560	T3276	T3254	cc	and,isolated
R2561	T3277	T3278	auxpass	were,cultured
R2562	T3278	T3253	conj	cultured,were
R2563	T3279	T3278	prep	at,cultured
R2564	T3280	T3283	nummod	1,cells
R2565	T3281	T3283	nummod	×,cells
R2566	T3282	T3283	nummod	106,cells
R2567	T3283	T3279	pobj	cells,at
R2568	T3284	T3283	prep	per,cells
R2569	T3285	T3286	advmod	well,in
R2570	T3286	T3284	prep	in,per
R2571	T3287	T3288	amod	6-well,plates
R2572	T3288	T3286	pobj	plates,in
R2573	T3289	T3283	prep	in,cells
R2574	T3290	T3291	compound	Macrophage-SFM,medium
R2575	T3291	T3289	pobj	medium,in
R2576	T3292	T3293	punct	(,Gibco
R2577	T3293	T3291	appos	Gibco,medium
R2578	T3294	T3293	punct	",",Gibco
R2579	T3295	T3293	appos	www.invitrogen.com,Gibco
R2580	T3296	T3293	punct	),Gibco
R2581	T3297	T3278	conj	supplemented,cultured
R2582	T3298	T3297	prep	with,supplemented
R2583	T3299	T3300	nummod	15,ng/ml
R2584	T3300	T3305	nmod	ng/ml,R&D
R2585	T3301	T3303	amod	recombinant,MCSF
R2586	T3302	T3303	compound	human,MCSF
R2587	T3303	T3300	appos	MCSF,ng/ml
R2588	T3304	T3305	punct	(,R&D
R2589	T3305	T3298	pobj	R&D,with
R2590	T3306	T3305	punct	",",R&D
R2591	T3307	T3305	appos	www.rndsystems.com,R&D
R2592	T3308	T3307	punct	),www.rndsystems.com
R2593	T3309	T3305	cc	and,R&D
R2594	T3310	T3311	nummod	5,%
R2595	T3311	T3315	nmod	%,serum
R2596	T3312	T3315	amod	heat-inactivated,serum
R2597	T3313	T3314	compound	human,AB
R2598	T3314	T3315	compound	AB,serum
R2599	T3315	T3305	conj	serum,R&D
R2600	T3316	T3317	punct	(,Nabi
R2601	T3317	T3315	appos	Nabi,serum
R2602	T3318	T3317	punct	",",Nabi
R2603	T3319	T3320	compound	Boca,Raton
R2604	T3320	T3317	conj	Raton,Nabi
R2605	T3321	T3320	punct	",",Raton
R2606	T3322	T3317	conj	FL,Nabi
R2607	T3323	T3317	punct	),Nabi
R2608	T3324	T3253	punct	.,were
R2609	T3325	T3327	det	The,cultures
R2610	T3326	T3327	compound	cell,cultures
R2611	T3327	T3329	nsubjpass	cultures,incubated
R2612	T3328	T3329	auxpass	were,incubated
R2613	T3329	T3329	ROOT	incubated,incubated
R2614	T3330	T3329	prep	at,incubated
R2615	T3331	T3332	nummod	37,°
R2616	T3332	T3333	nummod	°,C
R2617	T3333	T3330	pobj	C,at
R2618	T3334	T3329	cc	and,incubated
R2619	T3335	T3336	nummod	5,%
R2620	T3336	T3329	conj	%,incubated
R2621	T3337	T3346	nsubjpass	CO2,replaced
R2622	T3338	T3337	prep	for,CO2
R2623	T3339	T3340	nummod	5,days
R2624	T3340	T3338	pobj	days,for
R2625	T3341	T3337	punct	",",CO2
R2626	T3342	T3346	prep	after,replaced
R2627	T3343	T3342	pobj	which,after
R2628	T3344	T3346	nsubjpass	supernatant,replaced
R2629	T3345	T3346	auxpass	was,replaced
R2630	T3346	T3329	conj	replaced,incubated
R2631	T3347	T3346	prep	with,replaced
R2632	T3348	T3349	amod	fresh,medium
R2633	T3349	T3350	nsubj	medium,lacking
R2634	T3350	T3347	pcomp	lacking,with
R2635	T3351	T3350	dobj	MCSF,lacking
R2636	T3352	T3350	prep	before,lacking
R2637	T3353	T3352	pobj	manipulation,before
R2638	T3354	T3346	punct	.,replaced
R2639	T3355	T3357	amod	More,95
R2640	T3356	T3357	quantmod	than,95
R2641	T3357	T3358	nummod	95,%
R2642	T3358	T3363	nsubj	%,obtained
R2643	T3359	T3358	prep	of,%
R2644	T3360	T3362	det	the,cells
R2645	T3361	T3362	compound	adherent,cells
R2646	T3362	T3359	pobj	cells,of
R2647	T3363	T3363	ROOT	obtained,obtained
R2648	T3364	T3363	prep	with,obtained
R2649	T3365	T3366	det	this,technique
R2650	T3366	T3364	pobj	technique,with
R2651	T3367	T3363	auxpass	were,obtained
R2652	T3368	T3370	nummod	CD14,macrophages
R2653	T3369	T3370	compound	+,macrophages
R2654	T3370	T3363	nsubjpass	macrophages,obtained
R2655	T3371	T3372	mark	as,verified
R2656	T3372	T3370	advcl	verified,macrophages
R2657	T3373	T3372	agent	by,verified
R2658	T3374	T3373	pobj	flow,by
R2659	T3375	T3370	conj	cytometry,macrophages
R2660	T3376	T3363	punct	.,obtained
R2661	T3377	T3378	nummod	THP-1,cells
R2662	T3378	T3380	nsubjpass	cells,obtained
R2663	T3379	T3380	auxpass	were,obtained
R2664	T3380	T3380	ROOT	obtained,obtained
R2665	T3381	T3380	prep	from,obtained
R2666	T3382	T3381	pobj	ATCC,from
R2667	T3383	T3382	punct	(,ATCC
R2668	T3384	T3382	appos	www.atcc.org,ATCC
R2669	T3385	T3382	punct	),ATCC
R2670	T3386	T3380	cc	and,obtained
R2671	T3387	T3380	conj	cultured,obtained
R2672	T3388	T3387	prep	in,cultured
R2673	T3389	T3392	nsubj	RPMI,supplemented
R2674	T3390	T3391	nummod	1640,medium
R2675	T3391	T3392	nsubj	medium,supplemented
R2676	T3392	T3380	conj	supplemented,obtained
R2677	T3393	T3392	prep	with,supplemented
R2678	T3394	T3395	nummod	10,%
R2679	T3395	T3398	nmod	%,BioWhittaker
R2680	T3396	T3398	nmod	FCS,BioWhittaker
R2681	T3397	T3398	punct	(,BioWhittaker
R2682	T3398	T3393	pobj	BioWhittaker,with
R2683	T3399	T3398	punct	",",BioWhittaker
R2684	T3400	T3398	appos	www.lonzabio.com,BioWhittaker
R2685	T3401	T3398	punct	),BioWhittaker
R2686	T3402	T3392	punct	.,supplemented
R2687	T3403	T3404	nummod	293T,cells
R2688	T3404	T3406	nsubjpass	cells,obtained
R2689	T3405	T3406	auxpass	were,obtained
R2690	T3406	T3406	ROOT	obtained,obtained
R2691	T3407	T3406	prep	from,obtained
R2692	T3408	T3407	pobj	ATCC,from
R2693	T3409	T3408	punct	(,ATCC
R2694	T3410	T3408	appos	www.atcc.org,ATCC
R2695	T3411	T3408	punct	),ATCC
R2696	T3412	T3406	cc	and,obtained
R2697	T3413	T3414	auxpass	were,maintained
R2698	T3414	T3406	conj	maintained,obtained
R2699	T3415	T3414	prep	in,maintained
R2700	T3416	T3419	poss	Dulbecco,Eagle
R2701	T3417	T3416	case	's,Dulbecco
R2702	T3418	T3419	compound	Modified,Eagle
R2703	T3419	T3430	poss	Eagle,supplemented
R2704	T3420	T3419	case	's,Eagle
R2705	T3421	T3430	nsubj	medium,supplemented
R2706	T3422	T3423	punct	(,DMEM
R2707	T3423	T3421	appos	DMEM,medium
R2708	T3424	T3423	punct	),DMEM
R2709	T3425	T3426	punct	(,Gibco
R2710	T3426	T3423	appos	Gibco,DMEM
R2711	T3427	T3426	punct	",",Gibco
R2712	T3428	T3426	appos	www.invitrogen.com,Gibco
R2713	T3429	T3423	punct	),DMEM
R2714	T3430	T3406	conj	supplemented,obtained
R2715	T3431	T3430	prep	with,supplemented
R2716	T3432	T3433	nummod	10,%
R2717	T3433	T3434	compound	%,FCS
R2718	T3434	T3431	pobj	FCS,with
R2719	T3435	T3406	punct	.,obtained
R2787	T3534	T3534	ROOT	obtained,obtained
R2788	T3535	T3534	prep	from,obtained
R2789	T3536	T3538	det	The,Facility
R2790	T3537	T3538	compound	Centralized,Facility
R2791	T3538	T3535	pobj	Facility,from
R2792	T3539	T3538	prep	for,Facility
R2793	T3540	T3541	compound	AIDS,Reagents
R2794	T3541	T3539	pobj	Reagents,for
R2795	T3542	T3538	punct	",",Facility
R2796	T3543	T3544	compound	National,Institute
R2797	T3544	T3538	appos	Institute,Facility
R2798	T3545	T3544	prep	for,Institute
R2799	T3546	T3547	compound	Biological,Standard
R2800	T3547	T3545	pobj	Standard,for
R2801	T3548	T3547	cc	and,Standard
R2802	T3549	T3547	conj	Control,Standard
R2803	T3550	T3551	punct	(,NIBSC
R2804	T3551	T3547	appos	NIBSC,Standard
R2805	T3552	T3544	punct	),Institute
R2806	T3553	T3544	punct	",",Institute
R2807	T3554	T3555	compound	United,Kingdom
R2808	T3555	T3544	appos	Kingdom,Institute
R2809	T3556	T3534	punct	.,obtained
R2810	T3557	T3558	nsubj	HIV-1KR25,was
R2811	T3558	T3558	ROOT	was,was
R2812	T3559	T3558	acomp	isolated,was
R2813	T3560	T3559	prep	in,isolated
R2814	T3561	T3562	poss	our,laboratory
R2815	T3562	T3560	pobj	laboratory,in
R2816	T3563	T3564	mark	as,described
R2817	T3564	T3558	advcl	described,was
R2818	T3565	T3564	prep	before,described
R2819	T3566	T3565	pobj	[,before
R2820	T3567	T3568	nummod	33,]
R2821	T3568	T3566	appos	],[
R2822	T3569	T3558	punct	.,was
R3027	T3832	T3842	nsubjpass	LTR,constructed
R3028	T3833	T3834	compound	plasmid,construction
R3029	T3834	T3832	dobj	construction,LTR
R3030	T3835	T3834	cc	and,construction
R3031	T3836	T3837	compound	reporter,assay
R3032	T3837	T3840	compound	assay,plasmids
R3033	T3838	T3839	compound	LTR,reporter
R3034	T3839	T3840	compound	reporter,plasmids
R3035	T3840	T3834	conj	plasmids,construction
R3036	T3841	T3842	auxpass	were,constructed
R3037	T3842	T3842	ROOT	constructed,constructed
R3038	T3843	T3842	prep	by,constructed
R3039	T3844	T3843	pcomp	inserting,by
R3040	T3845	T3844	dobj	nucleotides,inserting
R3041	T3846	T3849	quantmod	−,+64
R3042	T3847	T3849	quantmod	208,+64
R3043	T3848	T3849	quantmod	to,+64
R3044	T3849	T3850	npadvmod	+64,relative
R3045	T3850	T3842	advmod	relative,constructed
R3046	T3851	T3850	prep	to,relative
R3047	T3852	T3855	det	the,site
R3048	T3853	T3855	amod	transcriptional,site
R3049	T3854	T3855	compound	initiation,site
R3050	T3855	T3851	pobj	site,to
R3051	T3856	T3855	prep	of,site
R3052	T3857	T3900	nsubj	HIV-1Lai,using
R3053	T3858	T3857	punct	",",HIV-1Lai
R3054	T3859	T3857	conj	HIV-1Bal,HIV-1Lai
R3055	T3860	T3862	punct	(,subtype
R3056	T3861	T3862	compound	B,subtype
R3057	T3862	T3859	appos	subtype,HIV-1Bal
R3058	T3863	T3862	punct	),subtype
R3059	T3864	T3859	punct	",",HIV-1Bal
R3060	T3865	T3859	conj	HIV-198IN17,HIV-1Bal
R3061	T3866	T3865	punct	",",HIV-198IN17
R3062	T3867	T3865	conj	HIV-198IN22,HIV-198IN17
R3063	T3868	T3867	punct	",",HIV-198IN22
R3064	T3869	T3867	conj	HIV-198CH01,HIV-198IN22
R3065	T3870	T3869	punct	",",HIV-198CH01
R3066	T3871	T3869	conj	HIV-1CM9,HIV-198CH01
R3067	T3872	T3874	punct	(,subtype
R3068	T3873	T3874	compound	C,subtype
R3069	T3874	T3871	parataxis	subtype,HIV-1CM9
R3070	T3875	T3874	punct	),subtype
R3071	T3876	T3871	punct	",",HIV-1CM9
R3072	T3877	T3871	conj	HIV-193TH64,HIV-1CM9
R3073	T3878	T3877	punct	",",HIV-193TH64
R3074	T3879	T3877	conj	HIV-192TH53,HIV-193TH64
R3075	T3880	T3879	punct	",",HIV-192TH53
R3076	T3881	T3879	conj	HIV-192TH51,HIV-192TH53
R3077	T3882	T3881	punct	",",HIV-192TH51
R3078	T3883	T3881	cc	and,HIV-192TH51
R3079	T3884	T3881	conj	HIV-1KR25,HIV-192TH51
R3080	T3885	T3887	punct	(,subtype
R3081	T3886	T3887	compound	E,subtype
R3082	T3887	T3884	appos	subtype,HIV-1KR25
R3083	T3888	T3887	punct	),subtype
R3084	T3889	T3857	prep	into,HIV-1Lai
R3085	T3890	T3892	det	the,vector
R3086	T3891	T3892	compound	reporter,vector
R3087	T3892	T3889	pobj	vector,into
R3088	T3893	T3892	appos	pGL3,vector
R3089	T3894	T3896	punct	(,BioSciences
R3090	T3895	T3896	compound	Promega,BioSciences
R3091	T3896	T3892	appos	BioSciences,vector
R3092	T3897	T3896	punct	",",BioSciences
R3093	T3898	T3896	appos	www.promega.com,BioSciences
R3094	T3899	T3896	punct	),BioSciences
R3095	T3900	T3856	pcomp	using,of
R3096	T3901	T3900	dobj	Xho,using
R3097	T3902	T3900	npadvmod	I,using
R3098	T3903	T3902	cc	and,I
R3099	T3904	T3905	compound	Hind,III
R3100	T3905	T3906	compound	III,restriction
R3101	T3906	T3908	compound	restriction,sites
R3102	T3907	T3908	compound	enzyme,sites
R3103	T3908	T3902	conj	sites,I
R3104	T3909	T3842	punct	.,constructed
R3105	T3910	T3912	nsubjpass	Sequences,aligned
R3106	T3911	T3912	auxpass	were,aligned
R3107	T3912	T3912	ROOT	aligned,aligned
R3108	T3913	T3912	cc	and,aligned
R3109	T3914	T3912	conj	analyzed,aligned
R3110	T3915	T3914	prep	with,analyzed
R3111	T3916	T3917	compound	CLUSTAL,W
R3112	T3917	T3915	pobj	W,with
R3113	T3918	T3917	punct	(,W
R3114	T3919	T3917	appos	www.ebi.ac.uk/clustalw/,W
R3115	T3920	T3917	punct	),W
R3116	T3921	T3912	punct	.,aligned
R3117	T3922	T3931	det	The,plasmid
R3118	T3923	T3931	amod	HIV-1Lai,plasmid
R3119	T3924	T3926	nummod	NFAT5,site-mutant
R3120	T3925	T3926	amod	binding,site-mutant
R3121	T3926	T3931	amod	site-mutant,plasmid
R3122	T3927	T3930	punct	(,reporter
R3123	T3928	T3930	amod	N5-Mut,reporter
R3124	T3929	T3930	punct	),reporter
R3125	T3930	T3931	compound	reporter,plasmid
R3126	T3931	T3933	nsubjpass	plasmid,created
R3127	T3932	T3933	auxpass	was,created
R3128	T3933	T3933	ROOT	created,created
R3129	T3934	T3933	agent	by,created
R3130	T3935	T3938	amod	standard,methods
R3131	T3936	T3938	amod	PCR-based,methods
R3132	T3937	T3938	compound	mutagenesis,methods
R3133	T3938	T3934	pobj	methods,by
R3134	T3939	T3941	nmod	[,]
R3135	T3940	T3941	nummod	34,]
R3136	T3941	T3938	appos	],methods
R3137	T3942	T3933	punct	.,created
R3138	T3943	T3944	nummod	THP-1,cells
R3139	T3944	T3951	nsubjpass	cells,transfected
R3140	T3945	T3947	punct	(,×
R3141	T3946	T3947	nummod	0.8,×
R3142	T3947	T3944	appos	×,cells
R3143	T3948	T3947	nummod	106/ml,×
R3144	T3949	T3947	punct	),×
R3145	T3950	T3951	auxpass	were,transfected
R3146	T3951	T3951	ROOT	transfected,transfected
R3147	T3952	T3951	prep	with,transfected
R3148	T3953	T3954	nummod	0.3,µg
R3149	T3954	T3987	amod	µg,www.qiagen.com
R3150	T3955	T3987	ccomp	/,www.qiagen.com
R3151	T3956	T3987	dep	ml,www.qiagen.com
R3152	T3957	T3958	compound	LTR,wild-type
R3153	T3958	T3956	appos	wild-type,ml
R3154	T3959	T3960	punct	(,WT
R3155	T3960	T3958	appos	WT,wild-type
R3156	T3961	T3960	punct	),WT
R3157	T3962	T3958	cc	or,wild-type
R3158	T3963	T3958	conj	mutated,wild-type
R3159	T3964	T3965	compound	reporter,plasmids
R3160	T3965	T3963	dobj	plasmids,mutated
R3161	T3966	T3963	prep	in,mutated
R3162	T3967	T3966	pobj	combination,in
R3163	T3968	T3963	prep	with,mutated
R3164	T3969	T3970	nummod	0.03,µg
R3165	T3970	T3979	nmod	µg,vector
R3166	T3971	T3974	nmod	/,luciferase
R3167	T3972	T3974	nmod	ml,luciferase
R3168	T3973	T3974	compound	Renilla,luciferase
R3169	T3974	T3979	nmod	luciferase,vector
R3170	T3975	T3976	punct	(,pRL-TK
R3171	T3976	T3974	appos	pRL-TK,luciferase
R3172	T3977	T3976	punct	),pRL-TK
R3173	T3978	T3979	compound	control,vector
R3174	T3979	T3980	nsubj	vector,using
R3175	T3980	T3968	pcomp	using,with
R3176	T3981	T3983	nmod	Effectene,reagent
R3177	T3982	T3983	compound	transfection,reagent
R3178	T3983	T3980	dobj	reagent,using
R3179	T3984	T3985	punct	(,Qiagen
R3180	T3985	T3983	appos	Qiagen,reagent
R3181	T3986	T3987	punct	;,www.qiagen.com
R3182	T3987	T3952	pobj	www.qiagen.com,with
R3183	T3988	T3987	punct	),www.qiagen.com
R3184	T3989	T3951	punct	.,transfected
R3185	T3990	T3992	nsubjpass	Cells,incubated
R3186	T3991	T3992	auxpass	were,incubated
R3187	T3992	T3992	ROOT	incubated,incubated
R3188	T3993	T3992	prep	at,incubated
R3189	T3994	T3995	nummod	37,°
R3190	T3995	T3996	nummod	°,C
R3191	T3996	T3993	pobj	C,at
R3192	T3997	T3992	prep	for,incubated
R3193	T3998	T3999	nummod	16,hours
R3194	T3999	T3997	pobj	hours,for
R3195	T4000	T4004	prep	after,stimulated
R3196	T4001	T4000	pobj	which,after
R3197	T4002	T4004	nsubjpass	they,stimulated
R3198	T4003	T4004	auxpass	were,stimulated
R3199	T4004	T3992	advcl	stimulated,incubated
R3200	T4005	T4004	prep	with,stimulated
R3201	T4006	T4007	nummod	10,µg
R3202	T4007	T4011	compound	µg,CDC1551
R3203	T4008	T4009	compound	/,ml
R3204	T4009	T4011	compound	ml,CDC1551
R3205	T4010	T4011	compound	MTb,CDC1551
R3206	T4011	T4005	pobj	CDC1551,with
R3207	T4012	T4004	advmod	lysate,stimulated
R3208	T4013	T4004	cc	or,stimulated
R3209	T4014	T4004	conj	left,stimulated
R3210	T4015	T4014	oprd	unstimulated,left
R3211	T4016	T4015	prep	for,unstimulated
R3212	T4017	T4018	nummod	8,hours
R3213	T4018	T4016	pobj	hours,for
R3214	T4019	T3992	punct	.,incubated
R3215	T4020	T4022	compound	Reporter,expression
R3216	T4021	T4022	compound	gene,expression
R3217	T4022	T4024	nsubjpass	expression,quantitated
R3218	T4023	T4024	auxpass	was,quantitated
R3219	T4024	T4024	ROOT	quantitated,quantitated
R3220	T4025	T4024	agent	by,quantitated
R3221	T4026	T4028	amod	dual-luciferase,assay
R3222	T4027	T4028	compound	reporter,assay
R3223	T4028	T4025	pobj	assay,by
R3224	T4029	T4024	prep	according,quantitated
R3225	T4030	T4029	prep	to,according
R3226	T4031	T4032	det	the,manufacturer
R3227	T4032	T4034	poss	manufacturer,protocol
R3228	T4033	T4032	case	's,manufacturer
R3229	T4034	T4030	pobj	protocol,to
R3230	T4035	T4036	punct	(,Promega
R3231	T4036	T4034	appos	Promega,protocol
R3232	T4037	T4038	punct	;,www.promega.com
R3233	T4038	T4038	ROOT	www.promega.com,www.promega.com
R3234	T4039	T4038	punct	),www.promega.com
R3235	T4040	T4024	punct	.,quantitated
R3317	T4213	T4214	compound	Quantitative,DNase
R3318	T4214	T4214	ROOT	DNase,DNase
R3320	T4216	T4214	acl	footprinting,DNase
R3321	T4217	T4218	amod	Recombinant,NFAT5
R3322	T4218	T4216	dobj	NFAT5,footprinting
R3323	T4219	T4218	punct	(,NFAT5
R3324	T4220	T4221	compound	amino,acids
R3325	T4221	T4218	appos	acids,NFAT5
R3326	T4222	T4218	parataxis	175,NFAT5
R3327	T4223	T4218	appos	471,NFAT5
R3328	T4224	T4218	punct	),NFAT5
R3329	T4225	T4216	prep	with,footprinting
R3330	T4226	T4229	det	an,×
R3331	T4227	T4229	amod	N-terminal,×
R3332	T4228	T4229	nummod	6,×
R3333	T4229	T4225	pobj	×,with
R3334	T4230	T4231	poss	His,tag
R3335	T4231	T4233	nsubjpass	tag,expressed
R3336	T4232	T4233	auxpass	was,expressed
R3337	T4233	T4233	ROOT	expressed,expressed
R3338	T4234	T4233	prep	in,expressed
R3339	T4235	T4237	compound	E.,BL21
R3340	T4236	T4237	compound	coli,BL21
R3341	T4237	T4234	pobj	BL21,in
R3342	T4238	T4237	punct	(,BL21
R3343	T4239	T4237	appos	DE3,BL21
R3344	T4240	T4237	punct	),BL21
R3345	T4241	T4234	pobj	cells,in
R3346	T4242	T4243	punct	(,Stratagene
R3347	T4243	T4241	appos	Stratagene,cells
R3348	T4244	T4245	punct	;,www.stratagene.com
R3349	T4245	T4241	conj	www.stratagene.com,cells
R3350	T4246	T4245	punct	),www.stratagene.com
R3351	T4247	T4245	cc	and,www.stratagene.com
R3352	T4248	T4241	acl	purified,cells
R3353	T4249	T4248	prep	under,purified
R3354	T4250	T4251	amod	native,conditions
R3355	T4251	T4249	pobj	conditions,under
R3356	T4252	T4251	acl	using,conditions
R3357	T4253	T4254	amod	Ni-NTA,agarose
R3358	T4254	T4252	dobj	agarose,using
R3359	T4255	T4254	punct	(,agarose
R3360	T4256	T4254	appos	Qiagen,agarose
R3361	T4257	T4254	punct	),agarose
R3362	T4258	T4233	punct	.,expressed
R3363	T4259	T4260	amod	Recombinant,p50
R3364	T4260	T4264	nsubjpass	p50,purchased
R3365	T4261	T4260	cc	and,p50
R3366	T4262	T4260	conj	p65,p50
R3367	T4263	T4264	auxpass	were,purchased
R3368	T4264	T4264	ROOT	purchased,purchased
R3369	T4265	T4269	punct	(,www.activemotif.com
R3370	T4266	T4267	amod	Active,Motif
R3371	T4267	T4269	nmod	Motif,www.activemotif.com
R3372	T4268	T4269	punct	",",www.activemotif.com
R3373	T4269	T4264	parataxis	www.activemotif.com,purchased
R3374	T4270	T4269	punct	),www.activemotif.com
R3375	T4271	T4264	punct	.,purchased
R3376	T4272	T4273	compound	Quantitative,DNase
R3377	T4273	T4277	nsubjpass	DNase,performed
R3378	T4274	T4277	nsubjpass	I,performed
R3379	T4275	T4277	nsubjpass	footprinting,performed
R3380	T4276	T4277	auxpass	was,performed
R3381	T4277	T4277	ROOT	performed,performed
R3382	T4278	T4280	mark	as,described
R3383	T4279	T4280	advmod	previously,described
R3384	T4280	T4277	advcl	described,performed
R3385	T4281	T4283	nmod	[,]
R3386	T4282	T4283	nummod	31,]
R3387	T4283	T4280	dobj	],described
R3388	T4284	T4277	punct	.,performed
R3511	T4485	T4488	nummod	HIV-1,clones
R3512	T4486	T4488	amod	infectious,clones
R3513	T4487	T4488	amod	molecular,clones
R3514	T4488	T4500	nsubjpass	clones,obtained
R3515	T4489	T4490	det	The,plasmid
R3516	T4490	T4500	nsubjpass	plasmid,obtained
R3517	T4491	T4490	acl	encoding,plasmid
R3518	T4492	T4496	det	the,clone
R3519	T4493	T4496	amod	full-length,clone
R3520	T4494	T4496	amod	infectious,clone
R3521	T4495	T4496	amod	molecular,clone
R3522	T4496	T4491	dobj	clone,encoding
R3523	T4497	T4496	prep	of,clone
R3524	T4498	T4497	pobj	HIV-1Lai,of
R3525	T4499	T4500	auxpass	was,obtained
R3526	T4500	T4500	ROOT	obtained,obtained
R3527	T4501	T4500	prep	from,obtained
R3528	T4502	T4505	det	the,Reagent
R3529	T4503	T4505	compound	NIH,Reagent
R3530	T4504	T4505	compound	AIDS,Reagent
R3531	T4505	T4501	pobj	Reagent,from
R3532	T4506	T4505	cc	and,Reagent
R3533	T4507	T4508	compound	Reference,Program
R3534	T4508	T4505	conj	Program,Reagent
R3535	T4509	T4500	punct	.,obtained
R3536	T4510	T4516	det	The,clone
R3537	T4511	T4516	amod	HIV-1Lai,clone
R3538	T4512	T4513	nsubj	/,Bal-Env
R3539	T4513	T4516	nmod	Bal-Env,clone
R3540	T4514	T4516	amod	infectious,clone
R3541	T4515	T4516	amod	molecular,clone
R3542	T4516	T4518	nsubjpass	clone,constructed
R3543	T4517	T4518	auxpass	was,constructed
R3544	T4518	T4518	ROOT	constructed,constructed
R3545	T4519	T4518	prep	by,constructed
R3546	T4520	T4519	pcomp	replacing,by
R3547	T4521	T4522	det	the,envelope
R3548	T4522	T4520	dobj	envelope,replacing
R3549	T4523	T4522	punct	(,envelope
R3550	T4524	T4522	appos	env,envelope
R3551	T4525	T4522	punct	),envelope
R3552	T4526	T4528	nummod	gp160,acids
R3553	T4527	T4528	compound	amino,acids
R3554	T4528	T4520	dobj	acids,replacing
R3555	T4529	T4520	npadvmod	103,replacing
R3556	T4530	T4520	dobj	717,replacing
R3557	T4531	T4530	prep	of,717
R3558	T4532	T4533	det	the,HIV-1Lai
R3559	T4533	T4542	nmod	HIV-1Lai,clone
R3560	T4534	T4536	punct	(,subtype
R3561	T4535	T4536	compound	B,subtype
R3562	T4536	T4533	appos	subtype,HIV-1Lai
R3563	T4537	T4538	nsubj	that,utilizes
R3564	T4538	T4536	relcl	utilizes,subtype
R3565	T4539	T4538	dobj	CXCR4,utilizes
R3566	T4540	T4536	punct	),subtype
R3567	T4541	T4542	amod	molecular,clone
R3568	T4542	T4531	pobj	clone,of
R3569	T4543	T4520	prep	with,replacing
R3570	T4544	T4546	det	the,region
R3571	T4545	T4546	amod	corresponding,region
R3572	T4546	T4543	pobj	region,with
R3573	T4547	T4546	prep	of,region
R3574	T4548	T4551	amod	HIV-1Bal,subtype
R3575	T4549	T4551	punct	(,subtype
R3576	T4550	T4551	compound	B,subtype
R3577	T4551	T4547	pobj	subtype,of
R3578	T4552	T4553	nsubj	that,utilizes
R3579	T4553	T4551	relcl	utilizes,subtype
R3580	T4554	T4553	dobj	CCR5,utilizes
R3581	T4555	T4551	punct	),subtype
R3582	T4556	T4518	punct	.,constructed
R3583	T4557	T4562	det	The,virus
R3584	T4558	T4562	amod	HIV-1Lai,virus
R3585	T4559	T4562	nmod	/,virus
R3586	T4560	T4562	nmod	Bal-Env,virus
R3587	T4561	T4562	amod	chimeric,virus
R3588	T4562	T4563	nsubj	virus,uses
R3589	T4563	T4563	ROOT	uses,uses
R3590	T4564	T4563	dobj	CCR5,uses
R3591	T4565	T4563	prep	as,uses
R3592	T4566	T4568	det	a,receptor
R3593	T4567	T4568	amod	secondary,receptor
R3594	T4568	T4565	pobj	receptor,as
R3595	T4569	T4563	punct	.,uses
R3596	T4570	T4573	det	The,clone
R3597	T4571	T4573	amod	infectious,clone
R3598	T4572	T4573	amod	molecular,clone
R3599	T4573	T4577	nsubjpass	clone,constructed
R3600	T4574	T4573	prep	of,clone
R3601	T4575	T4574	pobj	HIV-198IN22,of
R3602	T4576	T4577	auxpass	was,constructed
R3603	T4577	T4577	ROOT	constructed,constructed
R3604	T4578	T4577	advcl	using,constructed
R3605	T4579	T4578	dobj	DNA,using
R3606	T4580	T4579	acl	extracted,DNA
R3607	T4581	T4580	prep	from,extracted
R3608	T4582	T4581	pobj	PBMC,from
R3609	T4583	T4585	nsubjpass	that,infected
R3610	T4584	T4585	auxpass	were,infected
R3611	T4585	T4577	conj	infected,constructed
R3612	T4586	T4585	prep	with,infected
R3613	T4587	T4589	det	a,isolate
R3614	T4588	T4589	amod	primary,isolate
R3615	T4589	T4586	pobj	isolate,with
R3616	T4590	T4589	prep	of,isolate
R3617	T4591	T4590	pobj	HIV-198IN22,of
R3618	T4592	T4577	punct	.,constructed
R3619	T4593	T4595	amod	HIV-1Lai,Bal-Env
R3620	T4594	T4595	compound	/,Bal-Env
R3621	T4595	T4599	nmod	Bal-Env,viruses
R3622	T4596	T4595	cc	and,Bal-Env
R3623	T4597	T4595	conj	HIV-198IN22,Bal-Env
R3624	T4598	T4599	amod	mutant,viruses
R3625	T4599	T4601	nsubjpass	viruses,constructed
R3626	T4600	T4601	auxpass	were,constructed
R3627	T4601	T4601	ROOT	constructed,constructed
R3628	T4602	T4601	prep	by,constructed
R3629	T4603	T4602	pcomp	introducing,by
R3630	T4604	T4605	compound	point,mutations
R3631	T4605	T4603	dobj	mutations,introducing
R3632	T4606	T4603	advcl	using,introducing
R3633	T4607	T4610	amod	standard,methods
R3634	T4608	T4610	amod	PCR-based,methods
R3635	T4609	T4610	compound	mutagenesis,methods
R3636	T4610	T4606	dobj	methods,using
R3637	T4611	T4601	punct	.,constructed
R3809	T4857	T4858	compound	siRNA,transfection
R3810	T4858	T4864	nsubjpass	transfection,constructed
R3811	T4859	T4858	prep	of,transfection
R3812	T4860	T4859	pobj	MDM,of
R3813	T4861	T4862	det	An,siRNA
R3814	T4862	T4864	nsubjpass	siRNA,constructed
R3815	T4863	T4864	auxpass	was,constructed
R3816	T4864	T4864	ROOT	constructed,constructed
R3817	T4865	T4867	punct	(,Inc.
R3818	T4866	T4867	compound	Ambion,Inc.
R3819	T4867	T4864	oprd	Inc.,constructed
R3820	T4868	T4867	punct	",",Inc.
R3821	T4869	T4867	appos	www.ambion.com,Inc.
R3822	T4870	T4867	punct	),Inc.
R3823	T4871	T4872	aux	to,target
R3824	T4872	T4864	xcomp	target,constructed
R3825	T4873	T4874	det	a,sequence
R3826	T4874	T4872	dobj	sequence,target
R3827	T4875	T4874	amod	unique,sequence
R3828	T4876	T4875	prep	to,unique
R3829	T4877	T4879	det	the,transcript
R3830	T4878	T4879	compound	NFAT5,transcript
R3831	T4879	T4876	pobj	transcript,to
R3832	T4880	T4879	punct	:,transcript
R3833	T4881	T4885	nummod	5,′
R3834	T4882	T4884	punct	′,CAACATGCCTGGAATTCAA-3
R3835	T4883	T4884	punct	-,CAACATGCCTGGAATTCAA-3
R3836	T4884	T4885	compound	CAACATGCCTGGAATTCAA-3,′
R3837	T4885	T4879	appos	′,transcript
R3838	T4886	T4887	punct	(,nt
R3839	T4887	T4885	appos	nt,′
R3840	T4888	T4890	quantmod	335,353
R3841	T4889	T4890	quantmod	to,353
R3842	T4890	T4887	npadvmod	353,nt
R3843	T4891	T4879	punct	),transcript
R3844	T4892	T4876	pobj	[,to
R3845	T4893	T4894	nummod	31,]
R3846	T4894	T4892	dobj	],[
R3847	T4895	T4864	punct	.,constructed
R3848	T4896	T4897	mark	As,described
R3849	T4897	T4907	advcl	described,used
R3850	T4898	T4907	punct	",",used
R3851	T4899	T4900	det	a,control
R3852	T4900	T4907	npadvmod	control,used
R3853	T4901	T4900	prep	for,control
R3854	T4902	T4904	amod	non-specific,effects
R3855	T4903	T4904	compound	siRNA,effects
R3856	T4904	T4901	pobj	effects,for
R3857	T4905	T4907	punct	",",used
R3858	T4906	T4907	nsubj	we,used
R3859	T4907	T4907	ROOT	used,used
R3860	T4908	T4909	det	an,siRNA
R3861	T4909	T4907	dobj	siRNA,used
R3862	T4910	T4909	acl	targeting,siRNA
R3863	T4911	T4914	det	the,protein
R3864	T4912	T4914	amod	green,protein
R3865	T4913	T4914	amod	fluorescent,protein
R3866	T4914	T4910	dobj	protein,targeting
R3867	T4915	T4914	punct	(,protein
R3868	T4916	T4914	appos	GFP,protein
R3869	T4917	T4914	punct	),protein
R3870	T4918	T4914	punct	",",protein
R3871	T4919	T4922	nummod	5,GGCTACGTCCAGGAGCGCACC-3
R3872	T4920	T4922	punct	′,GGCTACGTCCAGGAGCGCACC-3
R3873	T4921	T4922	punct	-,GGCTACGTCCAGGAGCGCACC-3
R3874	T4922	T4923	compound	GGCTACGTCCAGGAGCGCACC-3,′
R3875	T4923	T4914	appos	′,protein
R3876	T4924	T4909	punct	.,siRNA
R3877	T4925	T4927	nsubjpass	MDM,transfected
R3878	T4926	T4927	auxpass	were,transfected
R3879	T4927	T4927	ROOT	transfected,transfected
R3880	T4928	T4927	prep	in,transfected
R3881	T4929	T4930	amod	6-well,plates
R3882	T4930	T4928	pobj	plates,in
R3883	T4931	T4927	advcl	using,transfected
R3884	T4932	T4933	nummod	1,µM
R3885	T4933	T4931	dobj	µM,using
R3886	T4934	T4933	prep	of,µM
R3887	T4935	T4937	det	the,siRNA
R3888	T4936	T4937	amod	indicated,siRNA
R3889	T4937	T4934	pobj	siRNA,of
R3890	T4938	T4937	prep	in,siRNA
R3891	T4939	T4942	nmod	siPORT,reagent
R3892	T4940	T4942	nmod	NeoFX,reagent
R3893	T4941	T4942	compound	transfection,reagent
R3894	T4942	T4945	nmod	reagent,Inc.
R3895	T4943	T4945	punct	(,Inc.
R3896	T4944	T4945	compound	Ambion,Inc.
R3897	T4945	T4938	pobj	Inc.,in
R3898	T4946	T4945	punct	",",Inc.
R3899	T4947	T4945	appos	www.ambion.com,Inc.
R3900	T4948	T4945	punct	),Inc.
R3901	T4949	T4933	punct	",",µM
R3902	T4950	T4927	conj	prepared,transfected
R3903	T4951	T4952	mark	as,recommended
R3904	T4952	T4950	advcl	recommended,prepared
R3905	T4953	T4952	agent	by,recommended
R3906	T4954	T4955	det	the,manufacturer
R3907	T4955	T4953	pobj	manufacturer,by
R3908	T4956	T4950	punct	",",prepared
R3909	T4957	T4950	prep	in,prepared
R3910	T4958	T4960	det	a,volume
R3911	T4959	T4960	amod	final,volume
R3912	T4960	T4957	pobj	volume,in
R3913	T4961	T4960	prep	of,volume
R3914	T4962	T4963	nummod	750,µl
R3915	T4963	T4961	pobj	µl,of
R3916	T4964	T4963	prep	in,µl
R3917	T4965	T4966	compound	Macrophage-SFM,medium
R3918	T4966	T4964	pobj	medium,in
R3919	T4967	T4927	cc	plus,transfected
R3920	T4968	T4969	nummod	5,%
R3921	T4969	T4973	nmod	%,serum
R3922	T4970	T4973	amod	heat-inactivated,serum
R3923	T4971	T4972	compound	human,AB
R3924	T4972	T4973	compound	AB,serum
R3925	T4973	T4927	conj	serum,transfected
R3926	T4974	T4927	punct	.,transfected
R3927	T4975	T4976	det	The,cultures
R3928	T4976	T4978	nsubjpass	cultures,left
R3929	T4977	T4978	auxpass	were,left
R3930	T4978	T4978	ROOT	left,left
R3931	T4979	T4978	prep	at,left
R3932	T4980	T4981	nummod	37,°
R3933	T4981	T4982	nummod	°,C
R3934	T4982	T4979	pobj	C,at
R3935	T4983	T4978	advmod	overnight,left
R3936	T4984	T4988	prep	after,washed
R3937	T4985	T4984	pobj	which,after
R3938	T4986	T4988	nsubjpass	cells,washed
R3939	T4987	T4988	auxpass	were,washed
R3940	T4988	T4978	advcl	washed,left
R3941	T4989	T4988	cc	and,washed
R3942	T4990	T4988	conj	incubated,washed
R3943	T4991	T4990	prep	in,incubated
R3944	T4992	T4993	amod	fresh,medium
R3945	T4993	T4991	pobj	medium,in
R3946	T4994	T4978	punct	.,left
R3947	T4995	T4997	nsubjpass	MDM,transfected
R3948	T4996	T4997	auxpass	were,transfected
R3949	T4997	T4997	ROOT	transfected,transfected
R3950	T4998	T4999	nummod	two,times
R3951	T4999	T4997	npadvmod	times,transfected
R3952	T5000	T4999	prep	for,times
R3953	T5001	T5002	nsubj	efficient,knock
R3954	T5002	T4997	conj	knock,transfected
R3955	T5003	T5002	advmod	down,knock
R3956	T5004	T5003	prep	of,down
R3957	T5005	T5006	nummod	NFAT5,expression
R3958	T5006	T5004	pobj	expression,of
R3959	T5007	T5011	mark	before,performed
R3960	T5008	T5009	compound	infection,experiments
R3961	T5009	T5011	nsubjpass	experiments,performed
R3962	T5010	T5011	auxpass	were,performed
R3963	T5011	T5002	advcl	performed,knock
R3964	T5012	T5014	nmod	[,]
R3965	T5013	T5014	nummod	31,]
R3966	T5014	T5011	dobj	],performed
R3967	T5015	T4997	punct	.,transfected
R4189	T5342	T5344	amod	Stable,cells
R4190	T5343	T5344	compound	THP-1,cells
R4191	T5344	T5344	ROOT	cells,cells
R4192	T5345	T5344	acl	expressing,cells
R4193	T5346	T5345	dobj	shRNA,expressing
R4194	T5347	T5349	det	The,plasmid
R4195	T5348	T5349	amod	lentiviral,plasmid
R4196	T5349	T5358	nsubjpass	plasmid,purchased
R4197	T5350	T5351	compound	pLKO,.1
R4198	T5351	T5358	nsubjpass	.1,purchased
R4199	T5352	T5351	advcl	expressing,.1
R4200	T5353	T5354	nmod	shRNA,targeting
R4201	T5354	T5358	nsubjpass	targeting,purchased
R4202	T5355	T5356	compound	human,MyD88
R4203	T5356	T5358	nsubjpass	MyD88,purchased
R4204	T5357	T5358	auxpass	was,purchased
R4205	T5358	T5358	ROOT	purchased,purchased
R4206	T5359	T5358	prep	from,purchased
R4207	T5360	T5361	compound	Open,Biosystems
R4208	T5361	T5359	pobj	Biosystems,from
R4209	T5362	T5361	punct	(,Biosystems
R4210	T5363	T5361	appos	www.openbiosystems.com,Biosystems
R4211	T5364	T5361	punct	),Biosystems
R4212	T5365	T5358	cc	and,purchased
R4213	T5366	T5367	auxpass	was,validated
R4214	T5367	T5358	conj	validated,purchased
R4215	T5368	T5367	prep	in,validated
R4216	T5369	T5370	poss	our,laboratory
R4217	T5370	T5368	pobj	laboratory,in
R4218	T5371	T5358	punct	.,purchased
R4219	T5372	T5429	nsubjpass	shRNA,designed
R4220	T5373	T5372	acl	targeting,shRNA
R4221	T5374	T5375	compound	human,IRAK1
R4222	T5375	T5373	dobj	IRAK1,targeting
R4223	T5376	T5378	punct	(,primer
R4224	T5377	T5378	advmod	forward,primer
R4225	T5378	T5373	parataxis	primer,targeting
R4226	T5379	T5382	nummod	5,CCGGAGCAGCTGTCCAGGTTTCGTCTCATAAAACCTGGACAGCTGCTCCTTTTTG-3
R4227	T5380	T5382	punct	′,CCGGAGCAGCTGTCCAGGTTTCGTCTCATAAAACCTGGACAGCTGCTCCTTTTTG-3
R4228	T5381	T5382	punct	-,CCGGAGCAGCTGTCCAGGTTTCGTCTCATAAAACCTGGACAGCTGCTCCTTTTTG-3
R4229	T5382	T5383	compound	CCGGAGCAGCTGTCCAGGTTTCGTCTCATAAAACCTGGACAGCTGCTCCTTTTTG-3,′
R4230	T5383	T5378	dobj	′,primer
R4231	T5384	T5378	punct	",",primer
R4232	T5385	T5390	ccomp	reverse,AATTCAAAAAGGAGCAGCTGTCCAGGTTTTATGAGACGAAACCTGGACAGCTGCT-3
R4233	T5386	T5385	dobj	primer,reverse
R4234	T5387	T5386	nummod	5,primer
R4235	T5388	T5390	punct	′,AATTCAAAAAGGAGCAGCTGTCCAGGTTTTATGAGACGAAACCTGGACAGCTGCT-3
R4236	T5389	T5390	punct	-,AATTCAAAAAGGAGCAGCTGTCCAGGTTTTATGAGACGAAACCTGGACAGCTGCT-3
R4237	T5390	T5392	amod	AATTCAAAAAGGAGCAGCTGTCCAGGTTTTATGAGACGAAACCTGGACAGCTGCT-3,mRNA
R4238	T5391	T5392	compound	′,mRNA
R4239	T5392	T5411	nmod	mRNA,′
R4240	T5393	T5399	punct	(,underlined
R4241	T5394	T5395	compound	IRAK1,mRNA
R4242	T5395	T5397	compound	mRNA,sequence
R4243	T5396	T5397	compound	target,sequence
R4244	T5397	T5399	nsubjpass	sequence,underlined
R4245	T5398	T5399	auxpass	is,underlined
R4246	T5399	T5392	parataxis	underlined,mRNA
R4247	T5400	T5399	punct	),underlined
R4248	T5401	T5399	cc	and,underlined
R4249	T5402	T5403	amod	human,TRAF6
R4250	T5403	T5392	conj	TRAF6,mRNA
R4251	T5404	T5406	punct	(,primer
R4252	T5405	T5406	advmod	forward,primer
R4253	T5406	T5392	appos	primer,mRNA
R4254	T5407	T5410	nummod	5,CCGGAGAAACCTGTTGTGATTCGTCTCATAAATCACAACAGGTTTCTCCTTTTTG-3
R4255	T5408	T5410	punct	′,CCGGAGAAACCTGTTGTGATTCGTCTCATAAATCACAACAGGTTTCTCCTTTTTG-3
R4256	T5409	T5410	punct	-,CCGGAGAAACCTGTTGTGATTCGTCTCATAAATCACAACAGGTTTCTCCTTTTTG-3
R4257	T5410	T5411	compound	CCGGAGAAACCTGTTGTGATTCGTCTCATAAATCACAACAGGTTTCTCCTTTTTG-3,′
R4258	T5411	T5378	dep	′,primer
R4259	T5412	T5378	punct	",",primer
R4260	T5413	T5429	nsubjpass	reverse,designed
R4261	T5414	T5419	nmod	primer,′
R4262	T5415	T5414	nummod	5,primer
R4263	T5416	T5418	punct	′,AATTCAAAAAGGAGAAACCTGTTGTGATTTATGAGACGAATCACAACAGGTTTCT-3
R4264	T5417	T5418	punct	-,AATTCAAAAAGGAGAAACCTGTTGTGATTTATGAGACGAATCACAACAGGTTTCT-3
R4265	T5418	T5419	compound	AATTCAAAAAGGAGAAACCTGTTGTGATTTATGAGACGAATCACAACAGGTTTCT-3,′
R4266	T5419	T5413	dobj	′,reverse
R4267	T5420	T5426	punct	(,underlined
R4268	T5421	T5424	nummod	TRAF6,sequence
R4269	T5422	T5424	compound	mRNA,sequence
R4270	T5423	T5424	compound	target,sequence
R4271	T5424	T5426	nsubjpass	sequence,underlined
R4272	T5425	T5426	auxpass	is,underlined
R4273	T5426	T5413	parataxis	underlined,reverse
R4274	T5427	T5426	punct	),underlined
R4275	T5428	T5429	auxpass	were,designed
R4276	T5429	T5429	ROOT	designed,designed
R4277	T5430	T5429	prep	in,designed
R4278	T5431	T5432	poss	our,laboratory
R4279	T5432	T5430	pobj	laboratory,in
R4280	T5433	T5429	cc	and,designed
R4281	T5434	T5435	auxpass	were,cloned
R4282	T5435	T5429	conj	cloned,designed
R4283	T5436	T5435	prep	into,cloned
R4284	T5437	T5440	det	the,plasmid
R4285	T5438	T5440	nmod	pLKO,plasmid
R4286	T5439	T5440	nummod	.1,plasmid
R4287	T5440	T5436	pobj	plasmid,into
R4288	T5441	T5429	punct	.,designed
R4289	T5442	T5447	nsubjpass	Lentiviruses,generated
R4290	T5443	T5445	amod	encoding,sequences
R4291	T5444	T5445	compound	shRNA,sequences
R4292	T5445	T5447	nsubjpass	sequences,generated
R4293	T5446	T5447	auxpass	were,generated
R4294	T5447	T5447	ROOT	generated,generated
R4295	T5448	T5447	prep	by,generated
R4296	T5449	T5448	pcomp	transfecting,by
R4297	T5450	T5454	det	the,HEK-293T
R4298	T5451	T5452	compound	packaging,cell
R4299	T5452	T5453	compound	cell,line
R4300	T5453	T5454	compound	line,HEK-293T
R4301	T5454	T5449	dobj	HEK-293T,transfecting
R4302	T5455	T5449	prep	with,transfecting
R4303	T5456	T5460	det	the,plasmids
R4304	T5457	T5460	amod	shRNA-encoding,plasmids
R4305	T5458	T5460	compound	pLKO,plasmids
R4306	T5459	T5460	nummod	.1,plasmids
R4307	T5460	T5455	pobj	plasmids,with
R4308	T5461	T5460	prep	in,plasmids
R4309	T5462	T5461	pobj	combination,in
R4310	T5463	T5460	prep	with,plasmids
R4311	T5464	T5465	det	the,packaging
R4312	T5465	T5466	compound	packaging,plasmid
R4313	T5466	T5467	compound	plasmid,psPAX2
R4314	T5467	T5463	pobj	psPAX2,with
R4315	T5468	T5467	cc	and,psPAX2
R4316	T5469	T5470	det	the,envelope
R4317	T5470	T5467	conj	envelope,psPAX2
R4318	T5471	T5448	pobj	plasmid,by
R4319	T5472	T5473	acomp	pMD2.G,using
R4320	T5473	T5471	advcl	using,plasmid
R4321	T5474	T5476	nmod	Effectene,reagent
R4322	T5475	T5476	compound	transfection,reagent
R4323	T5476	T5473	dobj	reagent,using
R4324	T5477	T5478	punct	(,Qiagen
R4325	T5478	T5476	appos	Qiagen,reagent
R4326	T5479	T5478	punct	",",Qiagen
R4327	T5480	T5478	appos	www.qiagen.com,Qiagen
R4328	T5481	T5478	punct	),Qiagen
R4329	T5482	T5447	punct	.,generated
R4330	T5483	T5485	nsubjpass	Supernatants,collected
R4331	T5484	T5485	auxpass	were,collected
R4332	T5485	T5485	ROOT	collected,collected
R4333	T5486	T5487	nummod	48,hours
R4334	T5487	T5488	compound	hours,post-transfection
R4335	T5488	T5485	oprd	post-transfection,collected
R4336	T5489	T5488	punct	",",post-transfection
R4337	T5490	T5488	acl	clarified,post-transfection
R4338	T5491	T5490	agent	by,clarified
R4339	T5492	T5491	pobj	centrifugation,by
R4340	T5493	T5485	punct	",",collected
R4341	T5494	T5485	cc	and,collected
R4342	T5495	T5485	conj	stored,collected
R4343	T5496	T5495	prep	at,stored
R4344	T5497	T5496	pobj	−,at
R4345	T5498	T5502	nummod	80,cells
R4346	T5499	T5501	compound	°,THP-1
R4347	T5500	T5501	compound	C.,THP-1
R4348	T5501	T5502	compound	THP-1,cells
R4349	T5502	T5504	nsubjpass	cells,transduced
R4350	T5503	T5504	auxpass	were,transduced
R4351	T5504	T5485	conj	transduced,collected
R4352	T5505	T5504	prep	with,transduced
R4353	T5506	T5508	det	the,particles
R4354	T5507	T5508	amod	lentiviral,particles
R4355	T5508	T5505	pobj	particles,with
R4356	T5509	T5504	prep	by,transduced
R4357	T5510	T5509	pcomp	culturing,by
R4358	T5511	T5512	det	the,cells
R4359	T5512	T5510	dobj	cells,culturing
R4360	T5513	T5510	prep	with,culturing
R4361	T5514	T5513	pobj	supernatants,with
R4362	T5515	T5514	prep	from,supernatants
R4363	T5516	T5518	det	the,cells
R4364	T5517	T5518	amod	virus-producing,cells
R4365	T5518	T5515	pobj	cells,from
R4366	T5519	T5518	prep	in,cells
R4367	T5520	T5521	det	the,presence
R4368	T5521	T5519	pobj	presence,in
R4369	T5522	T5521	prep	of,presence
R4370	T5523	T5522	pobj	8,of
R4371	T5524	T5535	meta	µg,for
R4372	T5525	T5527	nmod	/,polybrene
R4373	T5526	T5527	compound	ml,polybrene
R4374	T5527	T5524	appos	polybrene,µg
R4375	T5528	T5529	punct	(,Millipore
R4376	T5529	T5527	appos	Millipore,polybrene
R4377	T5530	T5529	punct	",",Millipore
R4378	T5531	T5529	appos	www.millipore.com,Millipore
R4379	T5532	T5531	punct	),www.millipore.com
R4380	T5533	T5529	cc	and,Millipore
R4381	T5534	T5535	meta	spinoculation,for
R4382	T5535	T5504	prep	for,transduced
R4383	T5536	T5537	nummod	two,hours
R4384	T5537	T5535	pobj	hours,for
R4385	T5538	T5537	prep	at,hours
R4386	T5539	T5540	nummod	2000,RPM
R4387	T5540	T5538	pobj	RPM,at
R4388	T5541	T5504	punct	.,transduced
R4389	T5542	T5543	advmod	Successfully,transduced
R4390	T5543	T5544	amod	transduced,cells
R4391	T5544	T5546	nsubjpass	cells,selected
R4392	T5545	T5546	auxpass	were,selected
R4393	T5546	T5546	ROOT	selected,selected
R4394	T5547	T5546	cc	and,selected
R4395	T5548	T5546	conj	expanded,selected
R4396	T5549	T5548	prep	by,expanded
R4397	T5550	T5549	pobj	treatment,by
R4398	T5551	T5548	prep	with,expanded
R4399	T5552	T5553	nummod	0.8,µg
R4400	T5553	T5556	nmod	µg,puromycin
R4401	T5554	T5556	nmod	/,puromycin
R4402	T5555	T5556	compound	ml,puromycin
R4403	T5556	T5551	pobj	puromycin,with
R4404	T5557	T5546	punct	.,selected
R4583	T5784	T5785	amod	MTb,culture
R4584	T5785	T5785	ROOT	culture,culture
R4585	T5786	T5789	det	The,strain
R4586	T5787	T5789	nmod	MTb,strain
R4587	T5788	T5789	amod	clinical,strain
R4588	T5789	T5792	nsubjpass	strain,prepared
R4589	T5790	T5792	nsubjpass	CDC1551,prepared
R4590	T5791	T5792	auxpass	was,prepared
R4591	T5792	T5792	ROOT	prepared,prepared
R4592	T5793	T5792	prep	by,prepared
R4593	T5794	T5793	pcomp	adding,by
R4594	T5795	T5796	nummod	100,µl
R4595	T5796	T5794	dobj	µl,adding
R4596	T5797	T5796	prep	of,µl
R4597	T5798	T5799	amod	frozen,bacteria
R4598	T5799	T5797	pobj	bacteria,of
R4599	T5800	T5796	conj	stock,µl
R4600	T5801	T5794	prep	into,adding
R4601	T5802	T5803	nummod	10,ml
R4602	T5803	T5801	pobj	ml,into
R4603	T5804	T5803	prep	of,ml
R4604	T5805	T5806	compound	Middlebrook,7H9
R4605	T5806	T5807	compound	7H9,medium
R4606	T5807	T5804	pobj	medium,of
R4607	T5808	T5810	punct	(,BD
R4608	T5809	T5810	compound	Difco,BD
R4609	T5810	T5803	appos	BD,ml
R4610	T5811	T5810	punct	",",BD
R4611	T5812	T5810	appos	www.bd.com,BD
R4612	T5813	T5810	punct	),BD
R4613	T5814	T5792	conj	supplemented,prepared
R4614	T5815	T5814	prep	with,supplemented
R4615	T5816	T5818	nmod	albumin,complex
R4616	T5817	T5818	compound	dextrose,complex
R4617	T5818	T5815	pobj	complex,with
R4618	T5819	T5820	punct	(,ADC
R4619	T5820	T5818	appos	ADC,complex
R4620	T5821	T5820	punct	),ADC
R4621	T5822	T5818	cc	and,complex
R4622	T5823	T5824	nummod	0.05,%
R4623	T5824	T5814	npadvmod	%,supplemented
R4624	T5825	T5826	nummod	Tween,80
R4625	T5826	T5828	nummod	80,Sigma-Aldrich
R4626	T5827	T5828	punct	(,Sigma-Aldrich
R4627	T5828	T5814	appos	Sigma-Aldrich,supplemented
R4628	T5829	T5828	punct	",",Sigma-Aldrich
R4629	T5830	T5828	appos	www.sigmaaldrich.com,Sigma-Aldrich
R4630	T5831	T5828	punct	),Sigma-Aldrich
R4631	T5832	T5792	punct	.,prepared
R4632	T5833	T5834	det	The,cultures
R4633	T5834	T5836	nsubjpass	cultures,grown
R4634	T5835	T5836	auxpass	were,grown
R4635	T5836	T5836	ROOT	grown,grown
R4636	T5837	T5836	prep	to,grown
R4637	T5838	T5839	det	an,OD650
R4638	T5839	T5837	pobj	OD650,to
R4639	T5840	T5839	prep	of,OD650
R4640	T5841	T5840	pobj	0.4,of
R4641	T5842	T5836	prep	at,grown
R4642	T5843	T5844	nummod	37,°
R4643	T5844	T5845	nummod	°,C
R4644	T5845	T5842	pobj	C,at
R4645	T5846	T5847	aux	to,ensure
R4646	T5847	T5836	advcl	ensure,grown
R4647	T5848	T5850	mark	that,were
R4648	T5849	T5850	nsubj	they,were
R4649	T5850	T5847	ccomp	were,ensure
R4650	T5851	T5850	prep	in,were
R4651	T5852	T5855	det	the,phase
R4652	T5853	T5855	amod	logarithmic,phase
R4653	T5854	T5855	compound	growth,phase
R4654	T5855	T5851	pobj	phase,in
R4655	T5856	T5836	punct	.,grown
R4656	T5857	T5860	nsubjpass	Bacteria,plated
R4657	T5858	T5860	auxpass	were,plated
R4658	T5859	T5860	advmod	then,plated
R4659	T5860	T5860	ROOT	plated,plated
R4660	T5861	T5860	punct	",",plated
R4661	T5862	T5860	conj	washed,plated
R4662	T5863	T5862	prep	with,washed
R4663	T5864	T5863	pobj	PBS,with
R4664	T5865	T5862	punct	",",washed
R4665	T5866	T5862	conj	resuspended,washed
R4666	T5867	T5866	prep	in,resuspended
R4667	T5868	T5867	pobj	PBS,in
R4668	T5869	T5866	punct	",",resuspended
R4669	T5870	T5866	cc	and,resuspended
R4670	T5871	T5866	conj	passed,resuspended
R4671	T5872	T5871	prep	through,passed
R4672	T5873	T5876	det	a,filter
R4673	T5874	T5875	nummod	5,µm
R4674	T5875	T5876	compound	µm,filter
R4675	T5876	T5872	pobj	filter,through
R4676	T5877	T5878	aux	to,ensure
R4677	T5878	T5871	advcl	ensure,passed
R4678	T5879	T5882	mark	that,were
R4679	T5880	T5881	det	the,bacteria
R4680	T5881	T5882	nsubj	bacteria,were
R4681	T5882	T5878	ccomp	were,ensure
R4682	T5883	T5882	prep	in,were
R4683	T5884	T5887	det	a,suspension
R4684	T5885	T5887	amod	single,suspension
R4685	T5886	T5887	compound	cell,suspension
R4686	T5887	T5883	pobj	suspension,in
R4687	T5888	T5860	punct	.,plated
R4688	T5889	T5891	amod	Bacterial,numbers
R4689	T5890	T5891	compound	cell,numbers
R4690	T5891	T5893	nsubjpass	numbers,determined
R4691	T5892	T5893	auxpass	were,determined
R4692	T5893	T5893	ROOT	determined,determined
R4693	T5894	T5893	agent	by,determined
R4694	T5895	T5894	pobj	measurement,by
R4695	T5896	T5895	prep	of,measurement
R4696	T5897	T5896	pobj	OD650,of
R4697	T5898	T5893	prep	before,determined
R4698	T5899	T5900	amod	further,dilution
R4699	T5900	T5898	pobj	dilution,before
R4700	T5901	T5900	prep	with,dilution
R4701	T5902	T5904	compound	RPMI,medium
R4702	T5903	T5904	compound	1640,medium
R4703	T5904	T5901	pobj	medium,with
R4704	T5905	T5904	prep	for,medium
R4705	T5906	T5907	compound	cell,infection
R4706	T5907	T5908	compound	infection,studies
R4707	T5908	T5905	pobj	studies,for
R4708	T5909	T5900	prep	at,dilution
R4709	T5910	T5911	nummod	10,∶
R4710	T5911	T5909	pobj	∶,at
R4711	T5912	T5915	nummod	1,bacilli
R4712	T5913	T5915	compound	PBMC,bacilli
R4713	T5914	T5915	compound	∶,bacilli
R4714	T5915	T5900	conj	bacilli,dilution
R4715	T5916	T5915	cc	or,bacilli
R4716	T5917	T5922	nummod	1,bacilli
R4717	T5918	T5922	nummod	∶,bacilli
R4718	T5919	T5922	nummod	1,bacilli
R4719	T5920	T5922	nmod	MDM,bacilli
R4720	T5921	T5922	compound	∶,bacilli
R4721	T5922	T5915	conj	bacilli,bacilli
R4722	T5923	T5922	cc	and,bacilli
R4723	T5924	T5926	nummod	THP-1,bacilli
R4724	T5925	T5926	compound	∶,bacilli
R4725	T5926	T5922	conj	bacilli,bacilli
R4726	T5927	T5893	punct	.,determined
R4727	T5928	T5929	amod	Colony-forming,unit
R4728	T5929	T5935	nsubjpass	unit,performed
R4729	T5930	T5929	punct	(,unit
R4730	T5931	T5929	appos	CFU,unit
R4731	T5932	T5933	punct	),analysis
R4732	T5933	T5935	nsubjpass	analysis,performed
R4733	T5934	T5935	auxpass	was,performed
R4734	T5935	T5935	ROOT	performed,performed
R4735	T5936	T5935	cc	and,performed
R4736	T5937	T5941	prep	on,7
R4737	T5938	T5937	pobj	days,on
R4738	T5939	T5938	nummod	4,days
R4739	T5940	T5938	cc	and,days
R4740	T5941	T5935	conj	7,performed
R4741	T5942	T5945	det	the,counts
R4742	T5943	T5945	amod	average,counts
R4743	T5944	T5945	compound	CFU,counts
R4744	T5945	T5946	nsubj	counts,were
R4745	T5946	T5935	conj	were,performed
R4746	T5947	T5948	nummod	6,×
R4747	T5948	T5946	attr	×,were
R4748	T5949	T5948	nummod	103,×
R4749	T5950	T5948	cc	and,×
R4750	T5951	T5953	nummod	5,104
R4751	T5952	T5953	nummod	×,104
R4752	T5953	T5948	conj	104,×
R4753	T5954	T5948	punct	",",×
R4754	T5955	T5948	advmod	respectively,×
R4755	T5956	T5946	punct	",",were
R4756	T5957	T5946	advcl	confirming,were
R4757	T5958	T5961	mark	that,increased
R4758	T5959	T5960	compound	mycobacteria,levels
R4759	T5960	T5961	nsubj	levels,increased
R4760	T5961	T5957	ccomp	increased,confirming
R4761	T5962	T5961	prep	over,increased
R4762	T5963	T5964	det	the,course
R4763	T5964	T5962	pobj	course,over
R4764	T5965	T5964	prep	of,course
R4765	T5966	T5965	pobj	infection,of
R4766	T5967	T5966	prep	of,infection
R4767	T5968	T5969	amod	primary,MDM
R4768	T5969	T5967	pobj	MDM,of
R4769	T5970	T5946	punct	.,were
R4992	T6246	T6250	amod	Western,extracts
R4993	T6247	T6250	amod	blot,extracts
R4994	T6248	T6250	amod	Whole,extracts
R4995	T6249	T6250	compound	cell,extracts
R4996	T6250	T6252	nsubjpass	extracts,collected
R4997	T6251	T6252	auxpass	were,collected
R4998	T6252	T6252	ROOT	collected,collected
R4999	T6253	T6252	prep	with,collected
R5000	T6254	T6255	compound	lysis,buffer
R5001	T6255	T6253	pobj	buffer,with
R5002	T6256	T6255	acl	containing,buffer
R5064	T6318	T6297	punct	.,boiled
R5065	T6319	T6320	det	The,gel
R5066	T6320	T6322	nsubjpass	gel,transferred
R5067	T6321	T6322	auxpass	was,transferred
R5068	T6322	T6322	ROOT	transferred,transferred
R5069	T6323	T6322	prep	to,transferred
R5070	T6324	T6328	det	a,Membrane
R5071	T6325	T6328	amod	nitrocellulose,Membrane
R5072	T6326	T6328	compound	Trans-Blot,Membrane
R5073	T6327	T6328	compound	Transfer,Membrane
R5074	T6328	T6323	pobj	Membrane,to
R5075	T6329	T6328	punct	(,Membrane
R5076	T6330	T6328	appos	BioRad,Membrane
R5077	T6331	T6328	punct	),Membrane
R5078	T6332	T6322	punct	.,transferred
R5079	T6333	T6334	det	The,blot
R5080	T6334	T6337	nsubjpass	blot,blocked
R5081	T6335	T6337	auxpass	was,blocked
R5082	T6336	T6337	advmod	then,blocked
R5083	T6337	T6337	ROOT	blocked,blocked
R5084	T6338	T6337	prep	for,blocked
R5085	T6339	T6340	nummod	1,h
R5086	T6340	T6338	pobj	h,for
R5087	T6341	T6337	prep	at,blocked
R5088	T6342	T6343	nummod	37,°
R5089	T6343	T6344	nummod	°,C
R5090	T6344	T6341	pobj	C,at
R5091	T6345	T6337	prep	in,blocked
R5092	T6346	T6347	det	a,solution
R5093	T6347	T6345	pobj	solution,in
R5094	T6348	T6347	prep	of,solution
R5095	T6349	T6350	nummod	4,%
R5096	T6350	T6348	pobj	%,of
R5097	T6351	T6348	pobj	BSA,of
R5098	T6352	T6353	punct	(,Sigma
R5099	T6353	T6351	appos	Sigma,BSA
R5100	T6354	T6353	punct	),Sigma
R5101	T6355	T6351	cc	and,BSA
R5102	T6356	T6357	nummod	0.1,%
R5103	T6357	T6358	compound	%,Tween-20
R5104	T6358	T6351	conj	Tween-20,BSA
R5105	T6359	T6360	punct	(,BioRad
R5106	T6360	T6358	appos	BioRad,Tween-20
R5107	T6361	T6360	punct	),BioRad
R5108	T6362	T6347	prep	in,solution
R5109	T6363	T6364	det	a,buffer
R5110	T6364	T6362	pobj	buffer,in
R5111	T6365	T6364	acl	containing,buffer
R5112	T6366	T6368	nummod	50,Tris
R5113	T6367	T6368	compound	mM,Tris
R5114	T6368	T6365	dobj	Tris,containing
R5115	T6369	T6368	cc	and,Tris
R5116	T6370	T6372	nummod	150,NaCl
R5117	T6371	T6372	compound	mM,NaCl
R5118	T6372	T6368	conj	NaCl,Tris
R5119	T6373	T6365	prep	at,containing
R5120	T6374	T6373	pobj	pH,at
R5121	T6375	T6374	nummod	7.6,pH
R5122	T6376	T6377	punct	(,BSA/TBST
R5123	T6377	T6374	appos	BSA/TBST,pH
R5124	T6378	T6374	punct	),pH
R5125	T6379	T6337	punct	.,blocked
R5126	T6380	T6381	amod	Primary,incubation
R5127	T6381	T6383	nsubjpass	incubation,carried
R5128	T6382	T6383	auxpass	was,carried
R5129	T6383	T6383	ROOT	carried,carried
R5130	T6384	T6383	prt	out,carried
R5131	T6385	T6383	prep	with,carried
R5132	T6386	T6389	nummod	a1,dilution
R5133	T6387	T6389	nummod	∶,dilution
R5134	T6388	T6389	nummod	200,dilution
R5135	T6389	T6385	pobj	dilution,with
R5136	T6390	T6389	prep	of,dilution
R5137	T6391	T6393	nmod	rabbit,antibody
R5138	T6392	T6393	amod	anti-NFAT5,antibody
R5139	T6393	T6390	pobj	antibody,of
R5140	T6394	T6393	punct	(,antibody
R5141	T6395	T6393	appos	H-300,antibody
R5142	T6396	T6393	punct	),antibody
R5143	T6397	T6400	punct	(,Biotechnology
R5144	T6398	T6399	compound	Santa,Cruz
R5145	T6399	T6400	compound	Cruz,Biotechnology
R5146	T6400	T6389	appos	Biotechnology,dilution
R5147	T6401	T6400	punct	),Biotechnology
R5148	T6402	T6389	cc	and,dilution
R5149	T6403	T6407	det	a,dilution
R5150	T6404	T6407	nummod	1,dilution
R5151	T6405	T6407	nmod	∶,dilution
R5152	T6406	T6407	nummod	500,dilution
R5153	T6407	T6389	conj	dilution,dilution
R5154	T6408	T6407	prep	of,dilution
R5155	T6409	T6411	nmod	goat,antibody
R5156	T6410	T6411	amod	anti-Lamin-B1,antibody
R5157	T6411	T6408	pobj	antibody,of
R5158	T6412	T6413	punct	(,sc-6217
R5160	T6414	T6407	punct	;,dilution
R5161	T6415	T6416	compound	Santa,Cruz
R5162	T6416	T6417	compound	Cruz,Biotechnology
R5163	T6417	T6407	appos	Biotechnology,dilution
R5164	T6418	T6407	punct	),dilution
R5165	T6419	T6407	prep	in,dilution
R5166	T6420	T6419	pobj	BSA/TBST,in
R5167	T6421	T6407	prep	for,dilution
R5168	T6422	T6423	nummod	2,h
R5169	T6423	T6421	pobj	h,for
R5170	T6424	T6423	prep	at,h
R5171	T6425	T6426	compound	room,temperature
R5172	T6426	T6424	pobj	temperature,at
R5173	T6427	T6383	punct	.,carried
R5174	T6428	T6429	det	The,blot
R5175	T6429	T6431	nsubjpass	blot,washed
R5176	T6430	T6431	auxpass	was,washed
R5177	T6431	T6431	ROOT	washed,washed
R5178	T6432	T6433	nummod	3,×
R5179	T6433	T6431	npadvmod	×,washed
R5180	T6434	T6435	nummod	5,min
R5181	T6435	T6433	appos	min,×
R5182	T6436	T6435	prep	in,min
R5183	T6437	T6436	pobj	TBST,in
R5184	T6438	T6435	cc	and,min
R5185	T6439	T6435	conj	incubated,min
R5186	T6440	T6439	prep	in,incubated
R5187	T6441	T6445	nummod	1,anti-goat-HRP
R5188	T6442	T6445	nummod	∶,anti-goat-HRP
R5189	T6443	T6445	nummod	6000,anti-goat-HRP
R5190	T6444	T6445	compound	donkey,anti-goat-HRP
R5191	T6445	T6440	pobj	anti-goat-HRP,in
R5192	T6446	T6449	punct	(,Biotechnology
R5193	T6447	T6449	compound	Santa,Biotechnology
R5194	T6448	T6449	compound	Cruz,Biotechnology
R5195	T6449	T6445	appos	Biotechnology,anti-goat-HRP
R5196	T6450	T6449	punct	),Biotechnology
R5197	T6451	T6445	cc	or,anti-goat-HRP
R5198	T6452	T6453	compound	goat,anti-rabbit-HRP
R5199	T6453	T6445	conj	anti-rabbit-HRP,anti-goat-HRP
R5200	T6454	T6455	punct	(,BioRad
R5201	T6455	T6453	appos	BioRad,anti-rabbit-HRP
R5202	T6456	T6453	punct	),anti-rabbit-HRP
R5203	T6457	T6445	prep	as,anti-goat-HRP
R5204	T6458	T6457	amod	appropriate,as
R5205	T6459	T6439	prep	for,incubated
R5206	T6460	T6461	nummod	1,h
R5207	T6461	T6459	pobj	h,for
R5208	T6462	T6431	punct	.,washed
R5209	T6463	T6464	det	The,blot
R5210	T6464	T6467	nsubjpass	blot,washed
R5211	T6465	T6467	auxpass	was,washed
R5212	T6466	T6467	advmod	again,washed
R5213	T6467	T6467	ROOT	washed,washed
R5214	T6468	T6471	nummod	3,min
R5215	T6469	T6471	nmod	×,min
R5216	T6470	T6469	nummod	5,×
R5217	T6471	T6467	dobj	min,washed
R5218	T6472	T6471	prep	in,min
R5219	T6473	T6472	pobj	TBST,in
R5220	T6474	T6467	cc	and,washed
R5221	T6475	T6467	conj	developed,washed
R5222	T6476	T6475	prep	with,developed
R5223	T6477	T6481	compound	SuperSignal,Reagent
R5224	T6478	T6479	compound	West,Pico
R5225	T6479	T6481	compound	Pico,Reagent
R5226	T6480	T6481	compound	Chemiluminescent,Reagent
R5227	T6481	T6476	pobj	Reagent,with
R5228	T6482	T6483	punct	(,Pierce
R5229	T6483	T6481	appos	Pierce,Reagent
R5230	T6484	T6481	punct	),Reagent
R5231	T6485	T6467	punct	.,washed
R5289	T6601	T6602	compound	Quantitative,PCR
R5290	T6602	T6602	ROOT	PCR,PCR
R5291	T6603	T6606	det	The,levels
R5292	T6604	T6606	compound	mRNA,levels
R5293	T6605	T6606	compound	expression,levels
R5294	T6606	T6608	nsubjpass	levels,determined
R5295	T6607	T6608	auxpass	were,determined
R5296	T6608	T6608	ROOT	determined,determined
R5297	T6609	T6608	agent	by,determined
R5298	T6610	T6613	nmod	SYBR,PCR
R5299	T6611	T6613	nmod	Green-based,PCR
R5300	T6612	T6613	amod	real-time,PCR
R5301	T6613	T6609	pobj	PCR,by
R5302	T6614	T6616	punct	(,Biosystems
R5303	T6615	T6616	compound	Applied,Biosystems
R5304	T6616	T6613	parataxis	Biosystems,PCR
R5305	T6617	T6616	punct	",",Biosystems
R5306	T6618	T6616	appos	www.appliedbiosystems.com,Biosystems
R5307	T6619	T6616	punct	),Biosystems
R5308	T6620	T6608	punct	.,determined
R5309	T6621	T6623	det	The,conditions
R5310	T6622	T6623	compound	reaction,conditions
R5311	T6623	T6624	nsubj	conditions,were
R5312	T6624	T6631	auxpass	were,followed
R5313	T6625	T6626	nummod	95,°
R5314	T6626	T6627	compound	°,C
R5315	T6627	T6631	npadvmod	C,followed
R5316	T6628	T6627	prep	for,C
R5317	T6629	T6630	nummod	10,min
R5318	T6630	T6628	pobj	min,for
R5319	T6631	T6631	ROOT	followed,followed
R5320	T6632	T6631	agent	by,followed
R5321	T6633	T6634	nummod	40,cycles
R5322	T6634	T6632	pobj	cycles,by
R5323	T6635	T6634	prep	of,cycles
R5324	T6636	T6638	nummod	95,C
R5325	T6637	T6638	compound	°,C
R5326	T6638	T6635	pobj	C,of
R5327	T6639	T6631	prep	for,followed
R5328	T6640	T6641	nummod	15,sec
R5329	T6641	T6639	pobj	sec,for
R5330	T6642	T6641	cc	and,sec
R5331	T6643	T6644	nummod	60,°
R5332	T6644	T6645	compound	°,C
R5333	T6645	T6641	conj	C,sec
R5334	T6646	T6641	prep	for,sec
R5335	T6647	T6648	nummod	1,min
R5336	T6648	T6646	pobj	min,for
R5337	T6649	T6631	punct	.,followed
R5338	T6650	T6651	det	The,results
R5339	T6651	T6652	nsubj	results,were
R5340	T6652	T6652	ROOT	were,were
R5341	T6653	T6652	acomp	normalized,were
R5342	T6654	T6653	advcl	using,normalized
R5343	T6655	T6656	compound	β-actin,mRNA
R5344	T6656	T6654	dobj	mRNA,using
R5345	T6657	T6654	prep	as,using
R5346	T6658	T6660	det	an,control
R5347	T6659	T6660	amod	internal,control
R5348	T6660	T6657	pobj	control,as
R5349	T6661	T6652	cc	and,were
R5350	T6662	T6652	conj	expressed,were
R5351	T6663	T6662	prep	as,expressed
R5352	T6664	T6665	amod	relative,values
R5353	T6665	T6663	pobj	values,as
R5354	T6666	T6652	punct	.,were
R5392	T6714	T6715	compound	Statistical,analysis
R5393	T6715	T6721	nsubj	analysis,expressed
R5394	T6716	T6717	advmod	Where,applicable
R5395	T6717	T6721	advcl	applicable,expressed
R5396	T6718	T6721	punct	",",expressed
R5397	T6719	T6721	nsubjpass	results,expressed
R5398	T6720	T6721	auxpass	are,expressed
R5399	T6721	T6721	ROOT	expressed,expressed
R5400	T6722	T6721	prep	as,expressed
R5401	T6723	T6725	amod	mean,SEM
R5402	T6724	T6725	compound	±,SEM
R5403	T6725	T6722	pobj	SEM,as
R5404	T6726	T6721	punct	.,expressed
R5405	T6727	T6732	nsubjpass	Comparison,performed
R5406	T6728	T6727	prep	between,Comparison
R5407	T6729	T6730	nummod	two,groups
R5408	T6730	T6728	pobj	groups,between
R5409	T6731	T6732	auxpass	was,performed
R5410	T6732	T6732	ROOT	performed,performed
R5411	T6733	T6732	advcl	using,performed
R5412	T6734	T6737	det	the,t-Test
R5413	T6735	T6737	amod	paired,t-Test
R5414	T6736	T6737	compound	Student,t-Test
R5415	T6737	T6733	dobj	t-Test,using
R5416	T6738	T6733	prep	with,using
R5417	T6739	T6740	det	the,aid
R5418	T6740	T6738	pobj	aid,with
R5419	T6741	T6740	prep	of,aid
R5420	T6742	T6743	compound	Microsoft,Excel
R5421	T6743	T6744	compound	Excel,software
R5422	T6744	T6741	pobj	software,of
R5423	T6745	T6732	punct	.,performed
R5424	T6746	T6747	nsubj	p,≤
R5425	T6747	T6747	ROOT	≤,≤
R5426	T6748	T6750	nsubjpass	0.05,considered
R5427	T6749	T6750	auxpass	was,considered
R5428	T6750	T6747	ccomp	considered,≤
R5429	T6751	T6750	oprd	significant,considered
R5430	T6752	T6747	punct	.,≤
R5636	T7032	T7033	nsubj	MTb,increases
R5637	T7033	T7033	ROOT	increases,increases
R5638	T7034	T7035	compound	HIV-1,LTR
R5639	T7035	T7036	compound	LTR,activity
R5640	T7036	T7033	dobj	activity,increases
R5641	T7037	T7036	prep	of,activity
R5642	T7038	T7040	compound	HIV-1,B
R5643	T7039	T7040	compound	subtypes,B
R5644	T7040	T7037	pobj	B,of
R5645	T7041	T7040	punct	",",B
R5646	T7042	T7040	conj	C,B
R5647	T7043	T7042	punct	",",C
R5648	T7044	T7042	cc	and,C
R5649	T7045	T7042	conj	E,C
R5650	T7046	T7047	aux	To,compare
R5651	T7047	T7033	advcl	compare,increases
R5652	T7048	T7050	det	the,impact
R5653	T7049	T7050	amod	functional,impact
R5654	T7050	T7047	dobj	impact,compare
R5655	T7051	T7050	prep	of,impact
R5656	T7052	T7053	compound	MTb,stimulation
R5657	T7053	T7051	pobj	stimulation,of
R5658	T7054	T7050	prep	on,impact
R5659	T7055	T7058	amod	subtype-specific,activity
R5660	T7056	T7057	compound	HIV-1,LTR
R5661	T7057	T7058	compound	LTR,activity
R5662	T7058	T7054	pobj	activity,on
R5663	T7059	T7062	punct	",",constructed
R5664	T7060	T7062	nsubj	we,constructed
R5665	T7061	T7062	advmod	first,constructed
R5666	T7062	T7089	parataxis	constructed,linked
R5667	T7063	T7064	compound	reporter,plasmids
R5668	T7064	T7062	dobj	plasmids,constructed
R5669	T7065	T7064	acl	containing,plasmids
R5670	T7066	T7068	amod	viral,B
R5671	T7067	T7068	compound	subtype,B
R5672	T7068	T7065	dobj	B,containing
R5673	T7069	T7068	punct	",",B
R5674	T7070	T7068	conj	C,B
R5675	T7071	T7070	punct	",",C
R5676	T7072	T7070	cc	and,C
R5677	T7073	T7074	compound	E,LTRs
R5678	T7074	T7070	conj	LTRs,C
R5679	T7075	T7074	punct	(,LTRs
R5680	T7076	T7074	nmod	−,LTRs
R5681	T7077	T7079	quantmod	208,+
R5682	T7078	T7079	quantmod	to,+
R5683	T7079	T7074	appos	+,LTRs
R5684	T7080	T7081	nummod	64,nt
R5685	T7081	T7086	dep	nt,start
R5686	T7082	T7081	amod	relative,nt
R5687	T7083	T7086	aux	to,start
R5688	T7084	T7085	det	the,transcription
R5689	T7085	T7086	nsubj	transcription,start
R5690	T7086	T7079	xcomp	start,+
R5691	T7087	T7086	dobj	site,start
R5692	T7088	T7062	punct	),constructed
R5693	T7089	T7089	ROOT	linked,linked
R5694	T7090	T7089	prep	to,linked
R5695	T7091	T7095	det	the,gene
R5696	T7092	T7095	amod	firefly,gene
R5697	T7093	T7095	compound	luciferase,gene
R5698	T7094	T7095	compound	reporter,gene
R5699	T7095	T7090	pobj	gene,to
R5700	T7096	T7033	punct	.,increases
R5701	T7097	T7111	prep	After,stimulated
R5702	T7098	T7097	pobj	transfection,After
R5703	T7099	T7098	prep	of,transfection
R5704	T7100	T7104	det	the,line
R5705	T7101	T7104	amod	monocytic,line
R5706	T7102	T7104	nummod	THP-1,line
R5707	T7103	T7104	compound	cell,line
R5708	T7104	T7099	pobj	line,of
R5709	T7105	T7104	prep	with,line
R5710	T7106	T7107	det	these,plasmids
R5711	T7107	T7105	pobj	plasmids,with
R5712	T7108	T7111	punct	",",stimulated
R5713	T7109	T7111	nsubjpass	cells,stimulated
R5714	T7110	T7111	auxpass	were,stimulated
R5715	T7111	T7111	ROOT	stimulated,stimulated
R5716	T7112	T7111	prep	with,stimulated
R5717	T7113	T7117	det	an,lysate
R5718	T7114	T7117	amod	irradiated,lysate
R5719	T7115	T7116	amod	whole,cell
R5720	T7116	T7117	compound	cell,lysate
R5721	T7117	T7112	pobj	lysate,with
R5722	T7118	T7117	prep	of,lysate
R5723	T7119	T7118	pobj	MTb,of
R5724	T7120	T7121	punct	(,H37Rv
R5725	T7121	T7119	appos	H37Rv,MTb
R5726	T7122	T7119	punct	),MTb
R5727	T7123	T7111	punct	.,stimulated
R5728	T7124	T7125	nsubj	We,note
R5729	T7125	T7125	ROOT	note,note
R5730	T7126	T7129	mark	that,induces
R5731	T7127	T7128	compound	MTb,lysate
R5732	T7128	T7129	nsubj	lysate,induces
R5733	T7129	T7125	ccomp	induces,note
R5734	T7130	T7131	amod	inflammatory,responses
R5735	T7131	T7129	dobj	responses,induces
R5736	T7132	T7131	prep	in,responses
R5737	T7133	T7132	pobj	monocytes,in
R5738	T7134	T7135	nsubj	that,resemble
R5739	T7135	T7133	relcl	resemble,monocytes
R5740	T7136	T7135	dobj	those,resemble
R5741	T7137	T7136	acl	induced,those
R5742	T7138	T7137	prep	in,induced
R5743	T7139	T7138	pobj	response,in
R5744	T7140	T7141	aux	to,live
R5745	T7141	T7139	acl	live,response
R5746	T7142	T7141	dobj	MTb,live
R5747	T7143	T7144	punct	(,see
R5748	T7144	T7125	parataxis	see,note
R5749	T7145	T7144	prep	for,see
R5750	T7146	T7145	pobj	example,for
R5751	T7147	T7144	punct	",",see
R5752	T7148	T7150	nmod	[,]
R5753	T7149	T7150	nummod	35,]
R5754	T7150	T7151	meta	],[
R5755	T7151	T7154	nmod	[,)
R5756	T7152	T7153	nummod	37,]
R5757	T7153	T7151	appos	],[
R5758	T7154	T7144	punct	),see
R5759	T7155	T7125	punct	.,note
R5760	T7156	T7168	prep	Upon,demonstrated
R5761	T7157	T7156	pobj	stimulation,Upon
R5762	T7158	T7168	punct	",",demonstrated
R5763	T7159	T7160	det	the,B
R5764	T7160	T7167	nmod	B,reporters
R5765	T7161	T7162	punct	",",C
R5766	T7162	T7160	conj	C,B
R5767	T7163	T7162	punct	",",C
R5768	T7164	T7162	cc	and,C
R5769	T7165	T7166	compound	E,LTR-driven
R5770	T7166	T7162	conj	LTR-driven,C
R5771	T7167	T7168	nsubj	reporters,demonstrated
R5772	T7168	T7168	ROOT	demonstrated,demonstrated
R5773	T7169	T7171	det	a,enhancement
R5774	T7170	T7171	amod	significant,enhancement
R5775	T7171	T7168	dobj	enhancement,demonstrated
R5776	T7172	T7171	prep	in,enhancement
R5777	T7173	T7174	compound	luciferase,activity
R5778	T7174	T7172	pobj	activity,in
R5779	T7175	T7176	punct	(,Figure
R5780	T7176	T7171	appos	Figure,enhancement
R5781	T7177	T7176	nummod	1A,Figure
R5782	T7178	T7176	punct	),Figure
R5783	T7179	T7171	cc	and,enhancement
R5784	T7180	T7181	det	the,magnitude
R5785	T7181	T7185	nsubj	magnitude,was
R5786	T7182	T7181	prep	of,magnitude
R5787	T7183	T7184	det	this,effect
R5788	T7184	T7182	pobj	effect,of
R5789	T7185	T7168	ccomp	was,demonstrated
R5790	T7186	T7185	acomp	subtype-specific,was
R5791	T7187	T7168	punct	.,demonstrated
R5792	T7188	T7190	compound	Subtype,LTRs
R5793	T7189	T7190	compound	C,LTRs
R5794	T7190	T7191	nsubj	LTRs,displayed
R5795	T7191	T7191	ROOT	displayed,displayed
R5796	T7192	T7194	det	the,activity
R5797	T7193	T7194	amod	strongest,activity
R5798	T7194	T7191	dobj	activity,displayed
R5799	T7195	T7191	punct	",",displayed
R5800	T7196	T7202	mark	while,isolates
R5801	T7197	T7198	det	the,LTRs
R5802	T7198	T7202	nsubj	LTRs,isolates
R5803	T7199	T7198	prep	from,LTRs
R5804	T7200	T7202	amod	subtype,isolates
R5805	T7201	T7202	compound	E,isolates
R5806	T7202	T7191	advcl	isolates,displayed
R5807	T7203	T7204	advmod	consistently,showed
R5808	T7204	T7202	ccomp	showed,isolates
R5809	T7205	T7207	det	the,activity
R5810	T7206	T7207	amod	weakest,activity
R5811	T7207	T7204	dobj	activity,showed
R5812	T7208	T7209	punct	(,Figure
R5813	T7209	T7207	appos	Figure,activity
R5814	T7210	T7209	nummod	1A,Figure
R5815	T7211	T7209	punct	),Figure
R5816	T7212	T7207	punct	",",activity
R5817	T7213	T7207	amod	consistent,activity
R5818	T7214	T7213	prep	with,consistent
R5819	T7215	T7216	amod	previous,studies
R5820	T7216	T7217	nsubj	studies,demonstrating
R5821	T7217	T7214	pcomp	demonstrating,with
R5822	T7218	T7220	amod	subtype-specific,activity
R5823	T7219	T7220	compound	LTR,activity
R5824	T7220	T7217	dobj	activity,demonstrating
R5825	T7221	T7222	nsubj	that,used
R5826	T7222	T7220	relcl	used,activity
R5827	T7223	T7222	dobj	TNF,used
R5828	T7224	T7222	prep	as,used
R5829	T7225	T7226	det	a,stimulus
R5830	T7226	T7224	pobj	stimulus,as
R5831	T7227	T7229	nmod	[,]
R5832	T7228	T7229	nummod	38,]
R5833	T7229	T7226	appos	],stimulus
R5834	T7230	T7229	punct	",",]
R5835	T7231	T7233	nmod	[,]
R5836	T7232	T7233	nummod	39,]
R5837	T7233	T7229	appos	],]
R5838	T7234	T7191	punct	.,displayed
R16014	T21717	T21715	pobj	LTR,with
R16015	T21718	T21718	ROOT	is,is
R16016	T21719	T21718	acomp	important,is
R16017	T21720	T21719	prep	for,important
R16018	T21721	T21723	amod	MTb-induced,transcription
R16019	T21722	T21723	compound	HIV-1,transcription
R16020	T21723	T21720	pobj	transcription,for
R16021	T21724	T21718	punct	.,is
R16022	T21725	T21730	punct	(,increases
R16023	T21726	T21730	advmod	A,increases
R16024	T21727	T21728	punct	),MTb
R16025	T21728	T21729	compound	MTb,stimulation
R16026	T21729	T21730	nsubj	stimulation,increases
R16027	T21730	T21730	ROOT	increases,increases
R16028	T21731	T21730	dobj	activity,increases
R16029	T21732	T21731	prep	of,activity
R16030	T21733	T21732	pobj	LTRs,of
R16031	T21734	T21733	acl	derived,LTRs
R16032	T21735	T21734	prep	from,derived
R16033	T21736	T21738	compound	HIV-1,B
R16034	T21737	T21738	compound	subtypes,B
R16035	T21738	T21735	pobj	B,from
R16036	T21739	T21740	punct	",",C
R16037	T21740	T21738	conj	C,B
R16038	T21741	T21740	punct	",",C
R16039	T21742	T21740	cc	and,C
R16040	T21743	T21745	compound	E.,LTRs
R16041	T21744	T21745	compound	HIV-1,LTRs
R16042	T21745	T21740	conj	LTRs,C
R16043	T21746	T21745	punct	(,LTRs
R16044	T21747	T21751	nmod	−,nt
R16045	T21748	T21750	quantmod	208,+64
R16046	T21749	T21750	quantmod	to,+64
R16047	T21750	T21751	nummod	+64,nt
R16048	T21751	T21771	nsubjpass	nt,cloned
R16049	T21752	T21751	amod	relative,nt
R16050	T21753	T21756	aux	to,start
R16051	T21754	T21755	det	the,transcription
R16052	T21755	T21756	nsubj	transcription,start
R16053	T21756	T21751	acl	start,nt
R16054	T21757	T21756	dobj	site,start
R16055	T21758	T21756	punct	),start
R16056	T21759	T21756	prep	from,start
R16057	T21760	T21762	compound	representative,B
R16058	T21761	T21762	compound	subtype,B
R16059	T21762	T21759	pobj	B,from
R16060	T21763	T21764	punct	",",C
R16061	T21764	T21762	appos	C,B
R16062	T21765	T21764	punct	",",C
R16063	T21766	T21764	cc	and,C
R16064	T21767	T21768	compound	E,viral
R16066	T21769	T21762	relcl	isolates,B
R16067	T21770	T21771	auxpass	were,cloned
R16068	T21771	T21771	ROOT	cloned,cloned
R16069	T21772	T21771	prep	into,cloned
R16070	T21773	T21774	amod	plasmid,pGL3
R16071	T21774	T21772	pobj	pGL3,into
R16072	T21775	T21771	punct	.,cloned
R16073	T21776	T21777	nummod	THP-1,cells
R16074	T21777	T21784	nsubjpass	cells,transfected
R16075	T21778	T21780	punct	(,×
R16076	T21779	T21780	nummod	0.8,×
R16077	T21780	T21777	appos	×,cells
R16078	T21781	T21780	nummod	106/ml,×
R16079	T21782	T21780	punct	),×
R16080	T21783	T21784	auxpass	were,transfected
R16081	T21784	T21784	ROOT	transfected,transfected
R16082	T21785	T21784	prep	with,transfected
R16083	T21786	T21788	det	each,plasmid
R16084	T21787	T21788	compound	reporter,plasmid
R16085	T21788	T21785	pobj	plasmid,with
R16086	T21789	T21788	punct	(,plasmid
R16087	T21790	T21791	nummod	0.3,µg
R16088	T21791	T21793	nmod	µg,ml
R16089	T21792	T21793	compound	/,ml
R16090	T21793	T21788	appos	ml,plasmid
R16091	T21794	T21793	punct	),ml
R16092	T21795	T21788	cc	plus,plasmid
R16093	T21796	T21799	det	the,control
R16094	T21797	T21799	nmod	Renilla,control
R16095	T21798	T21799	compound	luciferase,control
R16096	T21799	T21788	conj	control,plasmid
R16097	T21800	T21801	compound	plasmid,pRL-TK
R16098	T21801	T21784	npadvmod	pRL-TK,transfected
R16099	T21802	T21806	punct	(,ml
R16100	T21803	T21804	nummod	0.03,µg
R16101	T21804	T21806	nmod	µg,ml
R16102	T21805	T21806	compound	/,ml
R16103	T21806	T21801	appos	ml,pRL-TK
R16104	T21807	T21806	punct	),ml
R16105	T21808	T21784	cc	and,transfected
R16106	T21809	T21784	conj	incubated,transfected
R16107	T21810	T21809	prep	at,incubated
R16108	T21811	T21812	nummod	37,°
R16109	T21812	T21810	pobj	°,at
R16110	T21813	T21809	conj	C,incubated
R16111	T21814	T21813	prep	for,C
R16112	T21815	T21816	nummod	16,hours
R16113	T21816	T21814	pobj	hours,for
R16114	T21817	T21784	punct	.,transfected
R16115	T21818	T21819	nsubj	Cells,were
R16116	T21819	T21819	ROOT	were,were
R16117	T21820	T21819	advmod	then,were
R16118	T21821	T21822	preconj	either,left
R16119	T21822	T21819	conj	left,were
R16120	T21823	T21822	oprd	untreated,left
R16121	T21824	T21823	cc	or,untreated
R16122	T21825	T21823	conj	treated,untreated
R16123	T21826	T21825	prep	with,treated
R16124	T21827	T21828	nummod	10,µg
R16125	T21828	T21832	nmod	µg,lysate
R16126	T21829	T21832	nmod	/,lysate
R16127	T21830	T21832	compound	ml,lysate
R16128	T21831	T21832	compound	MTb,lysate
R16129	T21832	T21826	pobj	lysate,with
R16130	T21833	T21832	prep	for,lysate
R16131	T21834	T21835	nummod	8,hours
R16132	T21835	T21833	pobj	hours,for
R16133	T21836	T21823	prep	before,untreated
R16134	T21837	T21836	pobj	termination,before
R16135	T21838	T21837	prep	of,termination
R16136	T21839	T21840	det	the,cultures
R16137	T21840	T21838	pobj	cultures,of
R16138	T21841	T21819	punct	.,were
R16139	T21842	T21848	prep	In,represent
R16140	T21843	T21844	det	the,histogram
R16141	T21844	T21842	pobj	histogram,In
R16142	T21845	T21848	punct	",",represent
R16143	T21846	T21847	amod	open,bars
R16144	T21847	T21848	nsubj	bars,represent
R16145	T21848	T21848	ROOT	represent,represent
R16146	T21849	T21851	amod	individual,activities
R16147	T21850	T21851	compound	LTR,activities
R16148	T21851	T21848	dobj	activities,represent
R16149	T21852	T21851	prep	in,activities
R16150	T21853	T21854	amod	untreated,cells
R16151	T21854	T21852	pobj	cells,in
R16152	T21855	T21848	punct	.,represent
R16153	T21856	T21858	amod	Light,bars
R16154	T21857	T21858	amod	grey,bars
R16155	T21858	T21859	nsubj	bars,represent
R16156	T21859	T21859	ROOT	represent,represent
R16157	T21860	T21861	amod	mean,values
R16158	T21861	T21859	dobj	values,represent
R16159	T21862	T21861	prep	of,values
R16160	T21863	T21864	compound	LTR,activities
R16161	T21864	T21862	pobj	activities,of
R16162	T21865	T21861	prep	from,values
R16163	T21866	T21867	det	each,subtype
R16164	T21867	T21865	pobj	subtype,from
R16165	T21868	T21867	prep	in,subtype
R16166	T21869	T21870	amod	untreated,cells
R16167	T21870	T21868	pobj	cells,in
R16168	T21871	T21859	punct	.,represent
R16169	T21872	T21873	amod	Black,bars
R16170	T21873	T21874	nsubj	bars,represent
R16171	T21874	T21874	ROOT	represent,represent
R16172	T21875	T21877	amod	individual,activities
R16173	T21876	T21877	compound	LTR,activities
R16174	T21877	T21874	dobj	activities,represent
R16175	T21878	T21877	prep	in,activities
R16176	T21879	T21881	nmod	MTb,cells
R16177	T21880	T21881	amod	lysate-treated,cells
R16178	T21881	T21878	pobj	cells,in
R16179	T21882	T21877	punct	",",activities
R16180	T21883	T21877	cc	and,activities
R16181	T21884	T21886	amod	dark,bars
R16182	T21885	T21886	amod	grey,bars
R16183	T21886	T21877	conj	bars,activities
R16184	T21887	T21874	conj	represent,represent
R16185	T21888	T21889	amod	mean,values
R16186	T21889	T21887	dobj	values,represent
R16187	T21890	T21889	prep	of,values
R16188	T21891	T21892	compound	LTR,activities
R16189	T21892	T21890	pobj	activities,of
R16190	T21893	T21887	prep	from,represent
R16191	T21894	T21895	det	each,subtype
R16192	T21895	T21893	pobj	subtype,from
R16193	T21896	T21895	prep	in,subtype
R16194	T21897	T21896	pobj	cells,in
R16195	T21898	T21897	acl	treated,cells
R16196	T21899	T21898	prep	with,treated
R16197	T21900	T21899	pobj	MTb,with
R16198	T21901	T21893	pobj	lysate,from
R16199	T21902	T21874	punct	.,represent
R16200	T21903	T21915	nsubjpass	LTR,increased
R16201	T21904	T21905	amod	transcriptional,activity
R16202	T21905	T21903	dobj	activity,LTR
R16203	T21906	T21905	prep	for,activity
R16204	T21907	T21906	pobj	all,for
R16205	T21908	T21907	prep	of,all
R16206	T21909	T21910	det	the,representative
R16207	T21910	T21908	pobj	representative,of
R16208	T21911	T21912	amod	LTRs,tested
R16209	T21912	T21912	ROOT	tested,tested
R16210	T21913	T21915	auxpass	was,increased
R16211	T21914	T21915	advmod	significantly,increased
R16212	T21915	T21915	ROOT	increased,increased
R16213	T21916	T21915	prep	in,increased
R16214	T21917	T21916	pobj	cultures,in
R16215	T21918	T21917	acl	treated,cultures
R16216	T21919	T21918	prep	with,treated
R16217	T21920	T21919	pobj	MTb,with
R16218	T21921	T21915	conj	lysate,increased
R16219	T21922	T21921	prep	in,lysate
R16220	T21923	T21922	pobj	comparison,in
R16221	T21924	T21923	prep	to,comparison
R16222	T21925	T21926	amod	untreated,cultures
R16223	T21926	T21924	pobj	cultures,to
R16224	T21927	T21915	punct	.,increased
R16225	T21928	T21929	nsubj	Results,are
R16226	T21929	T21929	ROOT	are,are
R16227	T21930	T21929	prep	from,are
R16228	T21931	T21933	nummod	three,experiments
R16229	T21932	T21933	amod	independent,experiments
R16230	T21933	T21930	pobj	experiments,from
R16231	T21934	T21933	acl	performed,experiments
R16232	T21935	T21934	prep	in,performed
R16233	T21936	T21935	pobj	duplicate,in
R16234	T21937	T21940	punct	(,p
R16235	T21938	T21940	punct	*,p
R16236	T21939	T21940	punct	",",p
R16237	T21940	T21929	parataxis	p,are
R16238	T21941	T21940	dobj	<,p
R16239	T21942	T21941	nummod	0.05,<
R16240	T21943	T21940	punct	;,p
R16241	T21944	T21940	punct	**,p
R16242	T21945	T21947	punct	",",<
R16243	T21946	T21947	compound	p,<
R16244	T21947	T21948	compound	<,0.01
R16245	T21948	T21944	npadvmod	0.01,**
R16246	T21949	T21950	mark	as,compared
R16247	T21950	T21944	advcl	compared,**
R16248	T21951	T21950	prep	to,compared
R16249	T21952	T21953	compound	unstimulated,cultures
R16250	T21953	T21951	pobj	cultures,to
R16251	T21954	T21951	punct	),to
R16252	T21955	T21944	punct	.,**
R16253	T21956	T21957	punct	(,B
R16254	T21957	T21957	ROOT	B,B
R16255	T21958	T21957	punct	),B
R16256	T21959	T21960	amod	Specific,disruption
R16257	T21960	T21967	nsubj	disruption,reduces
R16258	T21961	T21960	prep	of,disruption
R16259	T21962	T21965	det	the,site
R16260	T21963	T21965	nmod	NFAT5,site
R16261	T21964	T21965	amod	binding,site
R16262	T21965	T21961	pobj	site,of
R16263	T21966	T21967	advmod	significantly,reduces
R16264	T21967	T21967	ROOT	reduces,reduces
R16265	T21968	T21970	amod	LTR-reporter,activity
R16266	T21969	T21970	compound	gene,activity
R16267	T21970	T21967	dobj	activity,reduces
R16268	T21971	T21970	prep	in,activity
R16269	T21972	T21973	amod	monocytic,cells
R16270	T21973	T21971	pobj	cells,in
R16271	T21974	T21967	prep	in,reduces
R16272	T21975	T21974	pobj	response,in
R16273	T21976	T21975	prep	to,response
R16274	T21977	T21979	nmod	MTb,treatment
R16275	T21978	T21979	amod	lysate,treatment
R16276	T21979	T21976	pobj	treatment,to
R16277	T21980	T21967	punct	.,reduces
R16278	T21981	T21982	nummod	THP-1,cells
R16279	T21982	T21984	nsubjpass	cells,transfected
R16280	T21983	T21984	auxpass	were,transfected
R16281	T21984	T21984	ROOT	transfected,transfected
R16282	T21985	T21984	prep	with,transfected
R16283	T21986	T21987	amod	luciferase,expression
R16284	T21987	T21988	compound	expression,vectors
R16285	T21988	T21989	nsubj	vectors,encoding
R16286	T21989	T21990	compound	encoding,nucleotides
R16287	T21990	T21985	pobj	nucleotides,with
R16288	T21991	T21984	advmod	208,transfected
R16289	T21992	T21991	prep	to,208
R16290	T21993	T21992	pobj	+64,to
R16291	T21994	T21993	prep	of,+64
R16292	T21995	T21998	det	the,LTR
R16293	T21996	T21998	amod	wild-type,LTR
R16294	T21997	T21998	compound	HIV-1Lai,LTR
R16295	T21998	T21994	pobj	LTR,of
R16296	T21999	T21993	cc	and,+64
R16297	T22000	T22002	det	an,LTR
R16298	T22001	T22002	compound	HIV-1Lai,LTR
R16299	T22002	T21991	appos	LTR,208
R16300	T22003	T22002	acl	containing,LTR
R16301	T22004	T22008	det	the,mutations
R16302	T22005	T22007	nmod	NFAT5,site
R16303	T22006	T22007	amod	binding,site
R16304	T22007	T22008	compound	site,mutations
R16305	T22008	T22003	dobj	mutations,containing
R16306	T22009	T22008	punct	(,mutations
R16307	T22010	T22008	appos	N5-Mut,mutations
R16308	T22011	T22008	punct	),mutations
R16309	T22012	T21984	punct	.,transfected
R16310	T22013	T22020	prep	After,left
R16311	T22014	T22015	nummod	16,hours
R16312	T22015	T22013	pobj	hours,After
R16313	T22016	T22020	punct	",",left
R16314	T22017	T22018	det	the,cells
R16315	T22018	T22020	nsubjpass	cells,left
R16316	T22019	T22020	auxpass	were,left
R16317	T22020	T22020	ROOT	left,left
R16318	T22021	T22020	oprd	untreated,left
R16319	T22022	T22020	cc	or,left
R16320	T22023	T22020	conj	exposed,left
R16321	T22024	T22023	prep	to,exposed
R16322	T22025	T22026	nummod	10,µg
R16323	T22026	T22030	nmod	µg,lysate
R16324	T22027	T22030	nmod	/,lysate
R16325	T22028	T22030	compound	ml,lysate
R16326	T22029	T22030	compound	MTb,lysate
R16327	T22030	T22024	pobj	lysate,to
R16328	T22031	T22023	prep	for,exposed
R16329	T22032	T22033	nummod	8,hours
R16330	T22033	T22031	pobj	hours,for
R16331	T22034	T22023	prep	at,exposed
R16332	T22035	T22038	nummod	37,Disruption
R16333	T22036	T22038	nummod	°,Disruption
R16334	T22037	T22038	compound	C.,Disruption
R16335	T22038	T22034	pobj	Disruption,at
R16336	T22039	T22038	prep	of,Disruption
R16337	T22040	T22039	pobj	NFAT5,of
R16338	T22041	T22023	conj	binding,exposed
R16339	T22042	T22041	prep	to,binding
R16340	T22043	T22045	det	the,region
R16341	T22044	T22045	compound	enhancer,region
R16342	T22045	T22042	pobj	region,to
R16343	T22046	T22047	advmod	significantly,suppressed
R16344	T22047	T22020	conj	suppressed,left
R16345	T22048	T22051	amod	LTR-driven,expression
R16346	T22049	T22050	compound	reporter,gene
R16347	T22050	T22051	compound	gene,expression
R16348	T22051	T22047	dobj	expression,suppressed
R16349	T22052	T22047	prep	in,suppressed
R16350	T22053	T22052	pobj	comparison,in
R16351	T22054	T22053	prep	to,comparison
R16352	T22055	T22057	det	the,LTR
R16353	T22056	T22057	amod	wild-type,LTR
R16354	T22057	T22054	pobj	LTR,to
R16355	T22058	T22061	advmod	when,treated
R16356	T22059	T22061	nsubjpass	cells,treated
R16357	T22060	T22061	auxpass	were,treated
R16358	T22061	T22057	relcl	treated,LTR
R16359	T22062	T22061	prep	with,treated
R16360	T22063	T22064	compound	MTb,lysate
R16361	T22064	T22062	pobj	lysate,with
R16362	T22065	T22067	punct	(,<
R16363	T22066	T22067	nmod	p,<
R16364	T22067	T22068	nmod	<,0.01
R16365	T22068	T22064	npadvmod	0.01,lysate
R16366	T22069	T22064	punct	),lysate
R16367	T22070	T22020	punct	.,left
R16368	T22071	T22072	compound	LTR,activity
R16369	T22072	T22075	nsubjpass	activity,suppressed
R16370	T22073	T22075	auxpass	was,suppressed
R16371	T22074	T22075	advmod	also,suppressed
R16372	T22075	T22075	ROOT	suppressed,suppressed
R16373	T22076	T22075	prep	in,suppressed
R16374	T22077	T22079	det	the,cells
R16375	T22078	T22079	amod	untreated,cells
R16376	T22079	T22076	pobj	cells,in
R16377	T22080	T22075	cc	but,suppressed
R16378	T22081	T22075	conj	to,suppressed
R16379	T22082	T22084	det	a,extent
R16380	T22083	T22084	amod	lesser,extent
R16381	T22084	T22081	pobj	extent,to
R16382	T22085	T22087	punct	(,<
R16383	T22086	T22087	nmod	p,<
R16384	T22087	T22088	nmod	<,0.05
R16385	T22088	T22084	appos	0.05,extent
R16386	T22089	T22084	punct	),extent
R16387	T22090	T22075	punct	.,suppressed
R16388	T22091	T22092	nsubj	Results,are
R16389	T22092	T22117	ccomp	are,<
R16390	T22093	T22092	prep	from,are
R16391	T22094	T22096	nummod	three,experiments
R16392	T22095	T22096	amod	independent,experiments
R16393	T22096	T22093	pobj	experiments,from
R16394	T22097	T22096	acl	performed,experiments
R16395	T22098	T22097	prep	in,performed
R16396	T22099	T22098	pobj	duplicate,in
R16397	T22100	T22097	cc	and,performed
R16398	T22101	T22097	conj	adjusted,performed
R16399	T22102	T22101	prep	to,adjusted
R16400	T22103	T22106	compound	Renilla,expression
R16401	T22104	T22105	compound	luciferase,control
R16402	T22105	T22106	compound	control,expression
R16403	T22106	T22102	pobj	expression,to
R16404	T22107	T22106	punct	(,expression
R16405	T22108	T22106	appos	*,expression
R16406	T22109	T22106	punct	",",expression
R16407	T22110	T22117	ccomp	p,<
R16408	T22111	T22112	compound	<,0.05
R16409	T22112	T22110	nummod	0.05,p
R16410	T22113	T22117	punct	;,<
R16411	T22114	T22117	punct	**,<
R16412	T22115	T22117	punct	",",<
R16413	T22116	T22117	amod	p,<
R16414	T22117	T22117	ROOT	<,<
R16415	T22118	T22117	nummod	0.01,<
R16416	T22119	T22117	punct	),<
R16417	T22120	T22117	punct	.,<
R16418	T22121	T22122	compound	Nucleotide,sequences
R16419	T22122	T22133	nsubjpass	sequences,shown
R16420	T22123	T22122	acl	representing,sequences
R16421	T22124	T22125	det	the,wild-type
R16422	T22125	T22123	dobj	wild-type,representing
R16423	T22126	T22125	cc	and,wild-type
R16424	T22127	T22131	nummod	NFAT5,LTRs
R16425	T22128	T22131	amod	binding,LTRs
R16426	T22129	T22131	amod	site-mutated,LTRs
R16427	T22130	T22131	amod	HIV-1Lai,LTRs
R16428	T22131	T22125	conj	LTRs,wild-type
R16429	T22132	T22133	auxpass	are,shown
R16430	T22133	T22133	ROOT	shown,shown
R16431	T22134	T22133	prep	at,shown
R16432	T22135	T22136	det	the,bottom
R16433	T22136	T22134	pobj	bottom,at
R16434	T22137	T22136	prep	of,bottom
R16435	T22138	T22139	det	the,figure
R16436	T22139	T22137	pobj	figure,of
R16437	T22140	T22133	punct	.,shown
R16438	T22141	T22142	punct	(,C
R16439	T22142	T22142	ROOT	C,C
R16440	T22143	T22142	punct	),C
R16441	T22144	T22145	compound	MTb,lysate
R16442	T22145	T22146	nsubj	lysate,increases
R16443	T22146	T22146	ROOT	increases,increases
R16444	T22147	T22149	nummod	NFAT5,levels
R16445	T22148	T22149	compound	protein,levels
R16446	T22149	T22146	dobj	levels,increases
R16447	T22150	T22149	prep	in,levels
R16448	T22151	T22152	amod	monocytic,cells
R16449	T22152	T22150	pobj	cells,in
R16450	T22153	T22146	punct	.,increases
R16451	T22154	T22155	nummod	THP-1,cells
R16452	T22155	T22157	nsubjpass	cells,left
R16453	T22156	T22157	auxpass	were,left
R16454	T22157	T22157	ROOT	left,left
R16455	T22158	T22157	oprd	untreated,left
R16456	T22159	T22160	punct	(,control
R16457	T22160	T22157	parataxis	control,left
R16458	T22161	T22160	punct	),control
R16459	T22162	T22160	cc	or,control
R16460	T22163	T22157	conj	exposed,left
R16461	T22164	T22163	prep	to,exposed
R16462	T22165	T22170	nummod	10,lysate
R16463	T22166	T22170	nmod	µg,lysate
R16464	T22167	T22170	nmod	/,lysate
R16465	T22168	T22170	compound	ml,lysate
R16466	T22169	T22170	compound	MTb,lysate
R16467	T22170	T22164	pobj	lysate,to
R16468	T22171	T22163	prep	for,exposed
R16469	T22172	T22175	nummod	8,hours
R16470	T22173	T22172	cc	or,8
R16471	T22174	T22172	conj	24,8
R16472	T22175	T22171	pobj	hours,for
R16473	T22176	T22175	prep	at,hours
R16474	T22177	T22182	nummod	37,extracts
R16475	T22178	T22182	nummod	°,extracts
R16476	T22179	T22180	compound	C.,Whole
R16477	T22180	T22182	compound	Whole,extracts
R16478	T22181	T22182	compound	cell,extracts
R16479	T22182	T22176	pobj	extracts,at
R16480	T22183	T22184	auxpass	were,collected
R16481	T22184	T22157	conj	collected,left
R16482	T22185	T22184	cc	and,collected
R16483	T22186	T22184	conj	analyzed,collected
R16484	T22187	T22186	agent	by,analyzed
R16485	T22188	T22189	amod	western,blot
R16486	T22189	T22187	pobj	blot,by
R16487	T22190	T22189	prep	with,blot
R2771	T3518	T3519	compound	Viruses,HIV-1Bal
R2772	T3519	T3534	nsubjpass	HIV-1Bal,obtained
R2773	T3520	T3519	punct	",",HIV-1Bal
R2774	T3521	T3519	conj	HIV-1Lai,HIV-1Bal
R2775	T3522	T3521	punct	",",HIV-1Lai
R2776	T3523	T3521	conj	HIV-193TH64,HIV-1Lai
R2777	T3524	T3523	punct	",",HIV-193TH64
R2778	T3525	T3523	conj	HIV-192TH51,HIV-193TH64
R2779	T3526	T3525	punct	",",HIV-192TH51
R2780	T3527	T3525	conj	HIV-192TH53,HIV-192TH51
R2781	T3528	T3527	punct	",",HIV-192TH53
R2782	T3529	T3527	conj	HIV-198CH01,HIV-192TH53
R2783	T3530	T3529	punct	",",HIV-198CH01
R2784	T3531	T3529	cc	and,HIV-198CH01
R2785	T3532	T3529	conj	HIV-198IN22,HIV-198CH01
R2786	T3533	T3534	auxpass	were,obtained
R5003	T6257	T6259	nummod	150,NaCl
R5004	T6258	T6259	compound	mM,NaCl
R5005	T6259	T6256	dobj	NaCl,containing
R5006	T6260	T6259	punct	",",NaCl
R5007	T6261	T6263	nummod	50,Tris
R5008	T6262	T6263	compound	mM,Tris
R5009	T6263	T6264	compound	Tris,HCl
R5010	T6264	T6259	appos	HCl,NaCl
R5011	T6265	T6264	punct	",",HCl
R5012	T6266	T6264	conj	pH,HCl
R5013	T6267	T6266	nummod	7.5,pH
R5014	T6268	T6266	punct	",",pH
R5015	T6269	T6270	nummod	1,%
R5016	T6270	T6271	compound	%,Triton
R5017	T6271	T6266	appos	Triton,pH
R5018	T6272	T6271	punct	",",Triton
R5019	T6273	T6274	nummod	10,%
R5020	T6274	T6275	compound	%,glycerol
R5021	T6275	T6271	conj	glycerol,Triton
R5022	T6276	T6275	punct	",",glycerol
R5023	T6277	T6275	cc	and,glycerol
R5024	T6278	T6279	nummod	1,tablet
R5025	T6279	T6275	conj	tablet,glycerol
R5026	T6280	T6279	prep	of,tablet
R5027	T6281	T6282	amod	Complete,EDTA-free
R5028	T6282	T6285	amod	EDTA-free,Cocktail
R5029	T6283	T6285	compound	Protease,Cocktail
R5030	T6284	T6285	compound	Inhibitor,Cocktail
R5031	T6285	T6280	pobj	Cocktail,of
R5032	T6286	T6287	punct	(,Roche
R5159	T6413	T6407	dep	sc-6217,dilution
R5033	T6287	T6285	appos	Roche,Cocktail
R5034	T6288	T6285	punct	),Cocktail
R5035	T6289	T6279	prep	per,tablet
R5036	T6290	T6291	nummod	25,ml
R5037	T6291	T6289	pobj	ml,per
R5038	T6292	T6291	prep	of,ml
R5039	T6293	T6292	pobj	buffer,of
R5040	T6294	T6252	punct	.,collected
R5041	T6295	T6297	nsubjpass	Extracts,boiled
R5042	T6296	T6297	auxpass	were,boiled
R5043	T6297	T6297	ROOT	boiled,boiled
R5044	T6298	T6297	prep	for,boiled
R5045	T6299	T6300	nummod	5,min
R5046	T6300	T6298	pobj	min,for
R5047	T6301	T6297	prep	in,boiled
R5048	T6302	T6303	nummod	1,×
R5049	T6303	T6301	pobj	×,in
R5050	T6304	T6306	compound	Laemmli,buffer
R5051	T6305	T6306	compound	sample,buffer
R5052	T6306	T6297	advcl	buffer,boiled
R5053	T6307	T6306	prep	with,buffer
R5054	T6308	T6309	nummod	5,%
R5055	T6309	T6311	nmod	%,2-mercaptoethanol
R5056	T6310	T6311	compound	v/v,2-mercaptoethanol
R5057	T6311	T6307	pobj	2-mercaptoethanol,with
R5058	T6312	T6311	cc	and,2-mercaptoethanol
R5059	T6313	T6315	nsubjpass	proteins,separated
R5060	T6314	T6315	auxpass	were,separated
R5061	T6315	T6311	conj	separated,2-mercaptoethanol
R5062	T6316	T6315	agent	by,separated
R5063	T6317	T6316	pobj	SDS-PAGE,by
R1414	T1932	T1928	punct	",",tuberculosis
R1417	T1933	T1935	det	the,agent
R1419	T1934	T1935	compound	causative,agent
R1420	T1935	T1928	appos	agent,tuberculosis
R1421	T1936	T1935	prep	of,agent
R1422	T1937	T1936	pobj	tuberculosis,of
R1423	T1938	T1937	punct	(,tuberculosis
R1424	T1939	T1937	appos	TB,tuberculosis
R1425	T1940	T1937	punct	),tuberculosis
R1426	T1941	T1928	punct	",",tuberculosis
R1427	T1942	T1942	ROOT	is,is
R1429	T1943	T1946	det	the,co-infection
R1431	T1944	T1945	advmod	most,common
R1435	T1945	T1946	amod	common,co-infection
R1437	T1946	T1942	attr	co-infection,is
R1439	T1947	T1946	cc	and,co-infection
R1442	T1948	T1946	conj	cause,co-infection
R1445	T1949	T1948	prep	of,cause
R1449	T1950	T1949	pobj	death,of
R1451	T1951	T1942	prep	in,is
R1453	T1952	T1951	pobj	patients,in
R1456	T1953	T1952	acl	infected,patients
R1460	T1954	T1953	prep	with,infected
R1464	T1955	T1956	amod	human,immunodeficiency
R1471	T1956	T1957	compound	immunodeficiency,virus
R1475	T1957	T1958	compound	virus,type
R1479	T1958	T1954	pobj	type,with
R1484	T1959	T1958	nummod	1,type
R1487	T1960	T1958	punct	(,type
R1492	T1961	T1958	appos	HIV-1,type
R1428	T1995	T1992	pobj	LTR,from
R1430	T1996	T1995	punct	(,LTR
R1432	T1997	T1995	acl	reviewed,LTR
R1433	T1998	T1997	prep	in,reviewed
R1434	T1999	T1998	pobj	[,in
R1436	T2000	T2001	nummod	3,]
R1438	T2001	T1999	appos	],[
R1440	T2002	T1999	punct	),[
R1441	T2003	T1985	punct	.,activates
R1443	T2004	T2020	advmod	Furthermore,activate
R1444	T2005	T2020	punct	",",activate
R1446	T2006	T2007	amod	inflammatory,cytokines
R1447	T2007	T2020	nsubj	cytokines,activate
R1448	T2008	T2007	cc	and,cytokines
R1450	T2009	T2007	conj	chemokines,cytokines
R1496	T1962	T1958	punct	),type
R1500	T1963	T1965	nmod	[,]
R1504	T1964	T1965	nummod	1,]
R1508	T1965	T1942	npadvmod	],is
R1510	T1966	T1965	punct	",",]
R1515	T1967	T1969	nmod	[,]
R1522	T1968	T1969	nummod	2,]
R1527	T1969	T1965	appos	],]
R1531	T1970	T1942	punct	.,is
R1534	T1971	T1972	amod	Direct,engagement
R1539	T1972	T1985	nsubj	engagement,activates
R1542	T1973	T1972	prep	of,engagement
R1546	T1974	T1975	compound	pathogen,recognition
R1550	T1975	T1976	compound	recognition,receptors
R1554	T1976	T1973	pobj	receptors,of
R1556	T1977	T1976	punct	(,receptors
R1558	T2089	T2085	punct	),]
R1860	T2401	T2400	punct	),NFAT
R1862	T2402	T2398	conj	family,cells
R1863	T2403	T2402	punct	",",family
R1864	T2404	T2390	appos	NFAT5,member
R1865	T2405	T2407	punct	(,known
R1867	T2406	T2407	advmod	also,known
R1868	T2407	T2390	acl	known,member
R1869	T2408	T2407	prep	as,known
R1870	T2409	T2408	pobj	TonEBP,as
R1871	T2410	T2390	punct	),member
R1873	T2411	T2412	punct	",",binds
R1874	T2412	T2385	ccomp	binds,showed
R1875	T2413	T2412	prep	to,binds
R1877	T2414	T2415	det	a,site
R1878	T2415	T2413	pobj	site,to
R1879	T2416	T2415	prep	within,site
R1881	T2417	T2419	det	the,LTR
R1883	T2418	T2419	compound	HIV-1,LTR
R1884	T2419	T2416	pobj	LTR,within
R1885	T2420	T2423	nsubjpass	that,conserved
R1887	T2421	T2423	auxpass	is,conserved
R1888	T2422	T2423	advmod	highly,conserved
R1889	T2423	T2415	relcl	conserved,site
R1890	T2424	T2423	prep	across,conserved
R1892	T2425	T2427	det	all,subtypes
R1893	T2426	T2427	compound	HIV-1,subtypes
R1894	T2427	T2424	pobj	subtypes,across
R1896	T2428	T2412	punct	",",binds
R1897	T2429	T2412	cc	and,binds
R1898	T2430	T2432	auxpass	is,conserved
R1901	T2431	T2432	advmod	also,conserved
R1903	T2432	T2412	conj	conserved,binds
R1904	T2433	T2432	prep	in,conserved
R1905	T2434	T2433	pobj	HIV-2,in
R1907	T2435	T2434	cc	and,HIV-2
R1908	T2436	T2437	compound	SIV,LTRs
R1909	T2437	T2434	conj	LTRs,HIV-2
R1911	T2438	T2385	punct	.,showed
R1912	T2439	T2441	det	This,site
R1913	T2440	T2441	nummod	NFAT5,site
R1915	T2441	T2442	nsubj	site,overlaps
R1916	T2442	T2442	ROOT	overlaps,overlaps
R1918	T2443	T2447	det	the,motifs
R1919	T2444	T2447	amod	core,motifs
R1920	T2445	T2447	amod	NF-κB,motifs
R1921	T2446	T2447	amod	binding,motifs
R1922	T2447	T2442	dobj	motifs,overlaps
R1923	T2448	T2447	prep	in,motifs
R1924	T2449	T2450	det	the,LTR
R1926	T2450	T2448	pobj	LTR,in
R1927	T2451	T2442	cc	and,overlaps
R1928	T2452	T2453	auxpass	is,required
R1930	T2453	T2442	conj	required,overlaps
R1931	T2454	T2453	prep	for,required
R1932	T2455	T2456	amod	constitutive,replication
R1934	T2456	T2454	pobj	replication,for
R1935	T2457	T2456	prep	of,replication
R16010	T21713	T21714	nummod	NFAT5,interaction
R16011	T21714	T21718	nsubj	interaction,is
R16012	T21715	T21714	prep	with,interaction
R16013	T21716	T21717	det	the,LTR
R16065	T21768	T21769	compound	viral,isolates
R3319	T4215	T4216	nsubj	I,footprinting

bionlp-st-ge-2016-test-tees

Id	Subject	Object	Predicate	Lexical cue
T21685	18805-18814	Positive_regulation	denotes	increases
T21684	18815-18828	Protein	denotes	NFAT5 protein
T21683	18710-18715	Protein	denotes	NFAT5
T21682	18622-18632	Gene_expression	denotes	expression
T21681	18595-18613	Protein	denotes	Renilla luciferase
T21680	18288-18298	Negative_regulation	denotes	suppressed
T21679	18324-18334	Gene_expression	denotes	expression
T21678	18243-18250	Binding	denotes	binding
T21677	18299-18323	Protein	denotes	LTR-driven reporter gene
T21676	18237-18242	Protein	denotes	NFAT5
T21675	17967-17977	Gene_expression	denotes	expression
T21674	17967-17977	Gene_expression	denotes	expression
T21673	17967-17977	Gene_expression	denotes	expression
T21672	17967-17977	Gene_expression	denotes	expression
T21671	18116-18119	Protein	denotes	Mut
T21670	18113-18115	Protein	denotes	N5
T21669	18083-18111	Protein	denotes	NFAT5 binding site mutations
T21668	17956-17966	Protein	denotes	luciferase
T21667	17780-17790	Transcription	denotes	disruption
T21666	17798-17816	Protein	denotes	NFAT5 binding site
T21665	16885-16903	Protein	denotes	Renilla luciferase
T21664	16477-16488	Binding	denotes	interaction
T21663	16471-16476	Protein	denotes	NFAT5
T7020	16397-16400	Protein	denotes	TNF
T7019	16168-16182	Protein	denotes	Subtype C LTRs
T7018	16066-16077	Positive_regulation	denotes	enhancement
T7017	16081-16091	Protein	denotes	luciferase
T7016	16016-16021	Protein	denotes	E LTR
T7015	15660-15684	Protein	denotes	luciferase reporter gene
T7014	15572-15578	Protein	denotes	E LTRs
T7013	15478-15487	Protein	denotes	HIV-1 LTR
T7012	15442-15445	Protein	denotes	MTb
T7011	15347-15356	Positive_regulation	denotes	increases
T7010	15357-15366	Protein	denotes	HIV-1 LTR
T4475	10224-10227	Protein	denotes	Env
T4474	10031-10035	Protein	denotes	CCR5
T4473	10007-10010	Protein	denotes	Env
T4472	9884-9892	Positive_regulation	denotes	utilizes
T4471	9983-9987	Protein	denotes	CCR5
T4470	9893-9898	Protein	denotes	CXCR4
T4469	9826-9831	Protein	denotes	gp160
T4468	9821-9824	Protein	denotes	env
T4467	9811-9819	Protein	denotes	envelope
T4203	9492-9499	Protein	denotes	DNase I
T4202	9403-9426	Protein	denotes	Recombinant p50 and p65
T4201	9265-9274	Gene_expression	denotes	expressed
T4200	9265-9274	Gene_expression	denotes	expressed
T4199	9257-9260	Protein	denotes	tag
T4198	9250-9252	Protein	denotes	6×
T4195	9170-9177	Protein	denotes	DNase I
T1926	5927-5937	Gene_expression	denotes	expression
T1925	6003-6008	Protein	denotes	NFAT5
T1924	5945-5948	Protein	denotes	PRR
T1923	5921-5926	Protein	denotes	NFAT5
T1922	5793-5802	Positive_regulation	denotes	increases
T1921	5814-5824	Gene_expression	denotes	expression
T1920	5849-5854	Protein	denotes	MyD88
T1919	5803-5813	Protein	denotes	NFAT5 gene
T1918	5606-5611	Protein	denotes	NFAT5
T1917	5426-5431	Protein	denotes	NFAT5
T1916	5384-5389	Protein	denotes	MyD88
T1915	5345-5359	Protein	denotes	MAP kinase p38
T1914	5272-5277	Protein	denotes	NFAT5
T1913	5055-5063	Binding	denotes	overlaps
T1912	5055-5063	Binding	denotes	overlaps
T1911	5068-5093	Protein	denotes	core NF-κB binding motifs
T1910	5044-5054	Protein	denotes	NFAT5 site
T156	1462-1465	Protein	denotes	TLR
T155	1453-1458	Protein	denotes	NFAT5
T154	1239-1246	Positive_regulation	denotes	induces
T153	1365-1376	Binding	denotes	interaction
T152	1270-1280	Gene_expression	denotes	expression
T151	1316-1319	Protein	denotes	MTb
T150	1286-1291	Protein	denotes	NFAT5
T149	1247-1257	Protein	denotes	NFAT5 gene
T148	1145-1153	Negative_regulation	denotes	inhibits
T147	1145-1153	Negative_regulation	denotes	inhibits
T146	1145-1153	Negative_regulation	denotes	inhibits
T145	1154-1165	Positive_regulation	denotes	MTb-induced
T144	1177-1187	Gene_expression	denotes	expression
T143	986-995	Negative_regulation	denotes	silencing
T142	986-995	Negative_regulation	denotes	silencing
T141	986-995	Negative_regulation	denotes	silencing
T140	1166-1176	Protein	denotes	NFAT5 gene
T139	1124-1129	Protein	denotes	TRAF6
T138	1113-1118	Protein	denotes	IRAK1
T137	1106-1111	Protein	denotes	MyD88
T136	1029-1032	Protein	denotes	TLR
T135	729-736	Positive_regulation	denotes	induced
T134	729-736	Positive_regulation	denotes	induced
T133	705-715	Gene_expression	denotes	expression
T132	808-826	Protein	denotes	NFAT5 binding site
T131	682-692	Protein	denotes	NFAT5 gene
T130	441-450	Negative_regulation	denotes	silencing
T129	441-450	Negative_regulation	denotes	silencing
T128	441-450	Negative_regulation	denotes	silencing
T127	458-468	Gene_expression	denotes	expression
T120	94-99	Protein	denotes	NFAT5
T121	107-125	Protein	denotes	Toll-Like Receptor
T122	476-511	Protein	denotes	transcription factor nuclear factor
T123	515-534	Protein	denotes	activated T cells 5
T124	536-541	Protein	denotes	NFAT5
T125	458-468	Gene_expression	denotes	expression
T126	458-468	Gene_expression	denotes	expression
T1905	4875-4880	Protein	denotes	NFAT5
T1904	4861-4865	Protein	denotes	NFAT
T1903	4714-4719	Protein	denotes	NF-κB
T1902	4527-4530	Binding	denotes	has
T1901	4535-4554	Protein	denotes	tandem NF-κB motifs
T1900	4121-4130	Binding	denotes	associate
T1899	3899-3908	Positive_regulation	denotes	activates
T1898	3899-3908	Positive_regulation	denotes	activates
T1897	3899-3908	Positive_regulation	denotes	activates
T1896	4140-4149	Protein	denotes	HIV-1 LTR
T1895	4081-4094	Protein	denotes	AP-1 families
T1894	4071-4076	Protein	denotes	NF-κB
T1893	3930-3969	Protein	denotes	mitogen-activated protein (MAP) kinases
T1892	3921-3924	Protein	denotes	IKK
T1891	3909-3919	Protein	denotes	IκB kinase
T1890	3893-3898	Protein	denotes	TRAF6
T1889	3766-3777	Binding	denotes	recruitment
T1888	3707-3717	Binding	denotes	engagement
T1887	3707-3717	Binding	denotes	engagement
T1886	3833-3849	Protein	denotes	ubiquitin ligase
T1885	3818-3823	Protein	denotes	IRAK4
T1884	3808-3813	Protein	denotes	IRAK1
T1883	3741-3746	Protein	denotes	MyD88
T1882	3284-3289	Protein	denotes	CD4 T
T1881	3053-3058	Protein	denotes	CD4 T
T1880	2715-2725	Binding	denotes	engagement
T1879	2715-2725	Binding	denotes	engagement
T1878	2761-2765	Protein	denotes	PRRs
T1877	2729-2759	Protein	denotes	pathogen recognition receptors
T1909	4905-4910	Binding	denotes	binds
T1908	4824-4859	Protein	denotes	nuclear factor of activated T cells
T1907	4932-4941	Protein	denotes	HIV-1 LTR
T1906	4896-4902	Protein	denotes	TonEBP
T4197	9191-9208	Protein	denotes	Recombinant NFAT5
T4196	9178-9190	Localization	denotes	footprinting
T5335	11801-11806	Protein	denotes	TRAF6
T5334	11632-11643	Protein	denotes	human TRAF6
T5333	11586-11591	Protein	denotes	IRAK1
T5332	11412-11423	Protein	denotes	human IRAK1
T5331	11260-11270	Gene_expression	denotes	expressing
T5330	11293-11298	Protein	denotes	MyD88
T4849	11118-11122	Negative_regulation	denotes	down
T4848	11132-11142	Gene_expression	denotes	expression
T4847	11126-11131	Protein	denotes	NFAT5
T4846	10761-10773	Protein	denotes	siPORT NeoFX
T4845	10650-10653	Protein	denotes	GFP
T4844	10623-10648	Protein	denotes	green fluorescent protein
T4843	10470-10486	Protein	denotes	NFAT5 transcript
T3823	9061-9071	Protein	denotes	luciferase
T3822	8788-8790	Protein	denotes	TK
T3821	8784-8787	Protein	denotes	pRL
T3820	8764-8782	Protein	denotes	Renilla luciferase
T3819	8516-8521	Protein	denotes	NFAT5
T3818	8374-8379	Positive_regulation	denotes	using
T3817	8390-8398	Protein	denotes	Hind III
T3816	8380-8385	Protein	denotes	Xho I
T6599	15033-15045	Protein	denotes	β-actin mRNA
T6245	14287-14292	Protein	denotes	NFAT5
T6244	13773-13791	Protein	denotes	Protease Inhibitor
T5780	12876-12911	Protein	denotes	Sigma-Aldrich, www.sigmaaldrich.com
T5779	12851-12854	Protein	denotes	ADC
T5778	12825-12849	Protein	denotes	albumin dextrose complex
T3188	7305-7310	Protein	denotes	CD14+
T3187	6735-6739	Protein	denotes	CD14
R22	T133	T135	themeOf	expression,induced
R3025	T3816	T3818	themeOf	Xho I,using
R3805	T4847	T4848	themeOf	NFAT5,expression
R3806	T4848	T4849	themeOf	expression,down
R4187	T5330	T5331	themeOf	MyD88,expressing
R15989	T21669	T21673	themeOf	NFAT5 binding site mutations,expression
R15990	T21670	T21674	themeOf	N5,expression
R19	T127	T130	themeOf	expression,silencing
R20	T131	T133	themeOf	NFAT5 gene,expression
R21	T131	T134	themeOf	NFAT5 gene,induced
R466	T122	T125	themeOf	transcription factor nuclear factor,expression
R467	T123	T126	themeOf	activated T cells 5,expression
R468	T124	T127	themeOf	NFAT5,expression
R469	T125	T128	themeOf	expression,silencing
R470	T126	T129	themeOf	expression,silencing
R23	T137	T141	themeOf	MyD88,silencing
R24	T138	T142	themeOf	IRAK1,silencing
R25	T139	T143	themeOf	TRAF6,silencing
R26	T140	T144	themeOf	NFAT5 gene,expression
R27	T141	T146	causeOf	silencing,inhibits
R28	T142	T147	causeOf	silencing,inhibits
R30	T144	T145	themeOf	expression,MTb-induced
R31	T145	T146	themeOf	MTb-induced,inhibits
R32	T145	T147	themeOf	MTb-induced,inhibits
R29	T143	T148	causeOf	silencing,inhibits
R33	T145	T148	themeOf	MTb-induced,inhibits
R34	T149	T152	themeOf	NFAT5 gene,expression
R35	T150	T153	themeOf	NFAT5,interaction
R36	T151	T153	themeOf	MTb,interaction
R37	T152	T154	themeOf	expression,induces
R1391	T1877	T1879	themeOf	pathogen recognition receptors,engagement
R1392	T1878	T1880	themeOf	PRRs,engagement
R1393	T1883	T1887	themeOf	MyD88,engagement
R1394	T1886	T1888	themeOf	ubiquitin ligase,engagement
R1395	T1886	T1889	themeOf	ubiquitin ligase,recruitment
R1396	T1891	T1897	themeOf	IκB kinase,activates
R1397	T1892	T1898	themeOf	IKK,activates
R1398	T1893	T1899	themeOf	mitogen-activated protein (MAP) kinases,activates
R1399	T1896	T1900	themeOf	HIV-1 LTR,associate
R1400	T1897	T1890	Cause	activates,TRAF6
R1401	T1898	T1890	Cause	activates,TRAF6
R1402	T1899	T1890	Cause	activates,TRAF6
R1403	T1901	T1902	themeOf	tandem NF-κB motifs,has
R1406	T1907	T1909	themeOf	HIV-1 LTR,binds
R1408	T1908	T1909	themeOf	nuclear factor of activated T cells,binds
R1409	T1910	T1912	themeOf	NFAT5 site,overlaps
R1410	T1910	T1913	themeOf	NFAT5 site,overlaps
R1412	T1911	T1913	themeOf	core NF-κB binding motifs,overlaps
R1415	T1919	T1921	themeOf	NFAT5 gene,expression
R1416	T1921	T1922	themeOf	expression,increases
R1418	T1923	T1926	themeOf	NFAT5,expression
R3311	T4195	T4196	themeOf	DNase I,footprinting
R3312	T4197	T4200	themeOf	Recombinant NFAT5,expressed
R3313	T4199	T4201	themeOf	tag,expressed
R3509	T4470	T4472	themeOf	CXCR4,utilizes
R5632	T7010	T7011	themeOf	HIV-1 LTR,increases
R5633	T7017	T7018	themeOf	luciferase,enhancement
R15986	T21663	T21664	themeOf	NFAT5,interaction
R15987	T21666	T21667	themeOf	NFAT5 binding site,disruption
R15988	T21668	T21672	themeOf	luciferase,expression
R15991	T21671	T21675	themeOf	Mut,expression
R15992	T21676	T21678	themeOf	NFAT5,binding
R15993	T21677	T21679	themeOf	LTR-driven reporter gene,expression
R15994	T21678	T21680	causeOf	binding,suppressed
R15995	T21679	T21680	themeOf	expression,suppressed
R15996	T21681	T21682	themeOf	Renilla luciferase,expression
R15997	T21684	T21685	themeOf	NFAT5 protein,increases

testone

Id	Subject	Object	Predicate	Lexical cue
T12	94-99	Protein	denotes	NFAT5
T21019	18805-18814	Positive_regulation	denotes	increases
T21018	18622-18632	Gene_expression	denotes	expression
T21017	18243-18250	Binding	denotes	binding
T21016	18223-18233	Negative_regulation	denotes	Disruption
T21015	17967-17977	Gene_expression	denotes	expression
T21014	16523-16530	Positive_regulation	denotes	induced
T21013	16477-16488	Binding	denotes	interaction
T21010	18815-18820	Protein	denotes	NFAT5
T21009	18603-18613	Protein	denotes	luciferase
T21008	18237-18242	Protein	denotes	NFAT5
T21007	17956-17966	Protein	denotes	luciferase
T21006	16893-16903	Protein	denotes	luciferase
T21005	16471-16476	Protein	denotes	NFAT5
T6759	16397-16400	Protein	denotes	TNF
T6758	16081-16091	Protein	denotes	luciferase
T6757	15660-15670	Protein	denotes	luciferase
T6676	15155-15164	Gene_expression	denotes	expressed
T6525	14771-14786	Transcription	denotes	mRNA expression
T6524	15033-15040	Protein	denotes	β-actin
T5988	14428-14431	Protein	denotes	BSA
T5987	14105-14108	Protein	denotes	BSA
T5052	11801-11806	Protein	denotes	TRAF6
T5051	11638-11643	Protein	denotes	TRAF6
T5050	11586-11591	Protein	denotes	IRAK1
T5049	11418-11423	Protein	denotes	IRAK1
T5048	11293-11298	Protein	denotes	MyD88
T5047	11271-11276	Protein	denotes	shRNA
T5046	11224-11229	Protein	denotes	shRNA
T4069	9265-9274	Gene_expression	denotes	expressed
T4068	9492-9499	Protein	denotes	DNase I
T4067	9423-9426	Protein	denotes	p65
T4066	9415-9418	Protein	denotes	p50
T4065	9203-9208	Protein	denotes	NFAT5
T4064	9170-9177	Protein	denotes	DNase I
T4636	11118-11122	Negative_regulation	denotes	down
T4635	11126-11131	Protein	denotes	NFAT5
T4634	10650-10653	Protein	denotes	GFP
T4633	10623-10648	Protein	denotes	green fluorescent protein
T4632	10470-10475	Protein	denotes	NFAT5
T4312	9983-9987	Protein	denotes	CCR5
T4311	9893-9898	Protein	denotes	CXCR4
T4310	9826-9831	Protein	denotes	gp160
T3578	9061-9071	Protein	denotes	luciferase
T2893	7305-7309	Protein	denotes	CD14
T2892	7209-7213	Protein	denotes	MCSF
T2891	6988-6992	Protein	denotes	MCSF
T2890	6735-6739	Protein	denotes	CD14
T3577	9012-9025	Protein	denotes	Reporter gene
T3576	8772-8782	Protein	denotes	luciferase
T879	5927-5937	Gene_expression	denotes	expression
T878	5793-5802	Positive_regulation	denotes	increases
T877	4905-4910	Binding	denotes	binds
T876	4121-4130	Binding	denotes	associate
T875	3766-3777	Binding	denotes	recruitment
T874	3707-3717	Binding	denotes	engagement
T873	3677-3687	Binding	denotes	Engagement
T872	6003-6008	Protein	denotes	NFAT5
T871	5921-5926	Protein	denotes	NFAT5
T870	5849-5854	Protein	denotes	MyD88
T869	5803-5808	Protein	denotes	NFAT5
T868	5606-5611	Protein	denotes	NFAT5
T867	5426-5431	Protein	denotes	NFAT5
T866	5384-5389	Protein	denotes	MyD88
T865	5272-5277	Protein	denotes	NFAT5
T864	4896-4902	Protein	denotes	TonEBP
T863	4875-4880	Protein	denotes	NFAT5
T862	3893-3898	Protein	denotes	TRAF6
T861	3850-3855	Protein	denotes	TRAF6
T860	3818-3823	Protein	denotes	IRAK4
T859	3808-3813	Protein	denotes	IRAK1
T858	3741-3746	Protein	denotes	MyD88
T857	3691-3695	Protein	denotes	TLR2
T856	3639-3665	Protein	denotes	toll-like receptor (TLR) 2
T855	3284-3287	Protein	denotes	CD4
T854	3053-3056	Protein	denotes	CD4
T31	1365-1376	Binding	denotes	interaction
T30	1270-1280	Gene_expression	denotes	expression
T29	1239-1246	Positive_regulation	denotes	induces
T28	1177-1187	Gene_expression	denotes	expression
T27	1158-1165	Positive_regulation	denotes	induced
T26	1145-1153	Negative_regulation	denotes	inhibits
T25	986-995	Negative_regulation	denotes	silencing
T24	729-736	Positive_regulation	denotes	induced
T23	441-450	Negative_regulation	denotes	silencing
T22	1453-1458	Protein	denotes	NFAT5
T21	1286-1291	Protein	denotes	NFAT5
T20	1247-1252	Protein	denotes	NFAT5
T19	1166-1171	Protein	denotes	NFAT5
T18	1124-1129	Protein	denotes	TRAF6
T17	1113-1118	Protein	denotes	IRAK1
T16	1106-1111	Protein	denotes	MyD88
T15	682-687	Protein	denotes	NFAT5
T14	536-541	Protein	denotes	NFAT5
T13	497-534	Protein	denotes	nuclear factor of activated T cells 5
R3	T18	T26	causeOf	TRAF6,inhibits
R4	T19	T28	themeOf	NFAT5,expression
R5	T20	T30	themeOf	NFAT5,expression
R6	T21	T31	themeOf	NFAT5,interaction
R7	T21	T29	themeOf	NFAT5,induces
R8	T27	T26	themeOf	induced,inhibits
R9	T28	T27	themeOf	expression,induced
R10	T30	T29	themeOf	expression,induces
R607	T857	T873	themeOf	TLR2,Engagement
R608	T857	T874	themeOf	TLR2,engagement
R609	T858	T874	themeOf	MyD88,engagement
R610	T859	T875	themeOf	IRAK1,recruitment
R611	T860	T875	themeOf	IRAK4,recruitment
R612	T861	T875	themeOf	TRAF6,recruitment
R613	T862	T876	themeOf	TRAF6,associate
R614	T863	T877	themeOf	NFAT5,binds
R615	T864	T877	themeOf	TonEBP,binds
R616	T871	T879	themeOf	NFAT5,expression
R3237	T4065	T4069	themeOf	NFAT5,expressed
R3638	T4634	T4633	equivalentTo	GFP,green fluorescent protein
R15456	T21005	T21013	themeOf	NFAT5,interaction
R15457	T21007	T21015	themeOf	luciferase,expression
R15458	T21008	T21017	themeOf	NFAT5,binding
R15459	T21009	T21018	themeOf	luciferase,expression
R15460	T21010	T21019	themeOf	NFAT5,increases
R15461	T21017	T21016	themeOf	binding,Disruption
R1	T14	T13	equivalentTo	NFAT5,nuclear factor of activated T cells 5
R2	T17	T26	causeOf	IRAK1,inhibits

test3

Id	Subject	Object	Predicate	Lexical cue
T32	94-99	Protein	denotes	NFAT5
T43	94-99	Protein	denotes	NFAT5
T21037	18815-18820	Protein	denotes	NFAT5
T21036	18622-18632	Gene_expression	denotes	expression
T21035	18603-18613	Protein	denotes	luciferase
T21034	18258-18266	Entity	denotes	enhancer
T21033	18243-18250	Binding	denotes	binding
T21032	18237-18242	Protein	denotes	NFAT5
T21031	17967-17977	Gene_expression	denotes	expression
T21030	17956-17966	Protein	denotes	luciferase
T21029	16893-16903	Protein	denotes	luciferase
T21028	16471-16476	Protein	denotes	NFAT5
T21025	18815-18820	Protein	denotes	NFAT5
T21024	18603-18613	Protein	denotes	luciferase
T21023	18237-18242	Protein	denotes	NFAT5
T21022	17956-17966	Protein	denotes	luciferase
T21021	16893-16903	Protein	denotes	luciferase
T21020	16471-16476	Protein	denotes	NFAT5
T6765	16397-16400	Protein	denotes	TNF
T6764	16081-16091	Protein	denotes	luciferase
T6763	15660-15670	Protein	denotes	luciferase
T6762	16397-16400	Protein	denotes	TNF
T6761	16081-16091	Protein	denotes	luciferase
T6760	15660-15670	Protein	denotes	luciferase
T6528	15033-15040	Protein	denotes	β-actin
T6527	14771-14786	Transcription	denotes	mRNA expression
T6526	15033-15040	Protein	denotes	β-actin
T5992	14428-14431	Protein	denotes	BSA
T5991	14105-14108	Protein	denotes	BSA
T5990	14428-14431	Protein	denotes	BSA
T5989	14105-14108	Protein	denotes	BSA
T5067	11801-11806	Protein	denotes	TRAF6
T5066	11638-11643	Protein	denotes	TRAF6
T5065	11586-11591	Protein	denotes	IRAK1
T5064	11418-11423	Protein	denotes	IRAK1
T5063	11293-11298	Protein	denotes	MyD88
T5062	11271-11276	Protein	denotes	shRNA
T5061	11224-11229	Protein	denotes	shRNA
T5060	11213-11223	Gene_expression	denotes	expressing
T5059	11801-11806	Protein	denotes	TRAF6
T5058	11638-11643	Protein	denotes	TRAF6
T5057	11586-11591	Protein	denotes	IRAK1
T5056	11418-11423	Protein	denotes	IRAK1
T5055	11293-11298	Protein	denotes	MyD88
T5054	11271-11276	Protein	denotes	shRNA
T5053	11224-11229	Protein	denotes	shRNA
T4318	9983-9987	Protein	denotes	CCR5
T4317	9893-9898	Protein	denotes	CXCR4
T4316	9826-9831	Protein	denotes	gp160
T4079	9492-9499	Protein	denotes	DNase I
T4078	9423-9426	Protein	denotes	p65
T4077	9415-9418	Protein	denotes	p50
T4076	9203-9208	Protein	denotes	NFAT5
T4075	9170-9177	Protein	denotes	DNase I
T4074	9492-9499	Protein	denotes	DNase I
T4073	9423-9426	Protein	denotes	p65
T4072	9415-9418	Protein	denotes	p50
T4071	9203-9208	Protein	denotes	NFAT5
T4070	9170-9177	Protein	denotes	DNase I
T4644	11126-11131	Protein	denotes	NFAT5
T4643	10650-10653	Protein	denotes	GFP
T4642	10623-10648	Protein	denotes	green fluorescent protein
T4641	10470-10475	Protein	denotes	NFAT5
T4640	11126-11131	Protein	denotes	NFAT5
T4639	10650-10653	Protein	denotes	GFP
T4638	10623-10648	Protein	denotes	green fluorescent protein
T4637	10470-10475	Protein	denotes	NFAT5
T4315	9983-9987	Protein	denotes	CCR5
T4314	9893-9898	Protein	denotes	CXCR4
T4313	9826-9831	Protein	denotes	gp160
T3584	9061-9071	Protein	denotes	luciferase
T3583	9012-9025	Protein	denotes	Reporter gene
T3582	8772-8782	Protein	denotes	luciferase
T3581	9061-9071	Protein	denotes	luciferase
T3580	9012-9025	Protein	denotes	Reporter gene
T3579	8772-8782	Protein	denotes	luciferase
T2901	7305-7309	Protein	denotes	CD14
T2900	7209-7213	Protein	denotes	MCSF
T2899	6988-6992	Protein	denotes	MCSF
T2898	6735-6739	Protein	denotes	CD14
T2897	7305-7309	Protein	denotes	CD14
T2896	7209-7213	Protein	denotes	MCSF
T2895	6988-6992	Protein	denotes	MCSF
T2894	6735-6739	Protein	denotes	CD14
T923	6003-6008	Protein	denotes	NFAT5
T922	5927-5937	Gene_expression	denotes	expression
T921	5921-5926	Protein	denotes	NFAT5
T920	5849-5854	Protein	denotes	MyD88
T919	5803-5808	Protein	denotes	NFAT5
T918	5606-5611	Protein	denotes	NFAT5
T917	5426-5431	Protein	denotes	NFAT5
T916	5384-5389	Protein	denotes	MyD88
T915	5328-5337	Positive_regulation	denotes	activated
T914	5272-5277	Protein	denotes	NFAT5
T913	4905-4910	Binding	denotes	binds
T912	4896-4902	Protein	denotes	TonEBP
T911	4875-4880	Protein	denotes	NFAT5
T910	3893-3898	Protein	denotes	TRAF6
T909	3850-3855	Protein	denotes	TRAF6
T908	3818-3823	Protein	denotes	IRAK4
T907	3808-3813	Protein	denotes	IRAK1
T906	3766-3777	Binding	denotes	recruitment
T905	3741-3746	Protein	denotes	MyD88
T904	3707-3717	Binding	denotes	engagement
T903	3691-3695	Protein	denotes	TLR2
T902	3677-3687	Binding	denotes	Engagement
T901	3639-3665	Protein	denotes	toll-like receptor (TLR) 2
T900	3284-3287	Protein	denotes	CD4
T899	3053-3056	Protein	denotes	CD4
T898	6003-6008	Protein	denotes	NFAT5
T897	5921-5926	Protein	denotes	NFAT5
T896	5849-5854	Protein	denotes	MyD88
T895	5803-5808	Protein	denotes	NFAT5
T894	5606-5611	Protein	denotes	NFAT5
T893	5426-5431	Protein	denotes	NFAT5
T892	5384-5389	Protein	denotes	MyD88
T891	5272-5277	Protein	denotes	NFAT5
T890	4896-4902	Protein	denotes	TonEBP
T889	4875-4880	Protein	denotes	NFAT5
T888	3893-3898	Protein	denotes	TRAF6
T887	3850-3855	Protein	denotes	TRAF6
T886	3818-3823	Protein	denotes	IRAK4
T885	3808-3813	Protein	denotes	IRAK1
T884	3741-3746	Protein	denotes	MyD88
T883	3691-3695	Protein	denotes	TLR2
T882	3639-3665	Protein	denotes	toll-like receptor (TLR) 2
T881	3284-3287	Protein	denotes	CD4
T880	3053-3056	Protein	denotes	CD4
T45	536-541	Protein	denotes	NFAT5
T44	497-534	Protein	denotes	nuclear factor of activated T cells 5
T42	1453-1458	Protein	denotes	NFAT5
T41	1286-1291	Protein	denotes	NFAT5
T40	1247-1252	Protein	denotes	NFAT5
T39	1166-1171	Protein	denotes	NFAT5
T38	1124-1129	Protein	denotes	TRAF6
T37	1113-1118	Protein	denotes	IRAK1
T36	1106-1111	Protein	denotes	MyD88
T35	682-687	Protein	denotes	NFAT5
T34	536-541	Protein	denotes	NFAT5
T33	497-534	Protein	denotes	nuclear factor of activated T cells 5
T56	1453-1458	Protein	denotes	NFAT5
T55	1365-1376	Binding	denotes	interaction
T54	1286-1291	Protein	denotes	NFAT5
T53	1270-1280	Gene_expression	denotes	expression
T52	1247-1252	Protein	denotes	NFAT5
T51	1166-1171	Protein	denotes	NFAT5
T50	1124-1129	Protein	denotes	TRAF6
T49	1113-1118	Protein	denotes	IRAK1
T48	1106-1111	Protein	denotes	MyD88
T47	729-736	Positive_regulation	denotes	induced
T46	682-687	Protein	denotes	NFAT5
R617	T903	T902	themeOf	TLR2,Engagement
R619	T905	T904	themeOf	MyD88,engagement
R620	T907	T906	themeOf	IRAK1,recruitment
R621	T908	T906	themeOf	IRAK4,recruitment
R622	T909	T906	themeOf	TRAF6,recruitment
R623	T911	T913	themeOf	NFAT5,binds
R624	T912	T913	themeOf	TonEBP,binds
R625	T914	T915	themeOf	NFAT5,activated
R626	T921	T922	themeOf	NFAT5,expression
R11	T45	T44	equivalentTo	NFAT5,nuclear factor of activated T cells 5
R12	T46	T47	themeOf	NFAT5,induced
R13	T52	T55	themeOf	NFAT5,interaction
R14	T52	T53	themeOf	NFAT5,expression
R15	T54	T55	themeOf	NFAT5,interaction
R618	T905	T906	themeOf	MyD88,recruitment
R3639	T4643	T4642	equivalentTo	GFP,green fluorescent protein
R3972	T5061	T5060	themeOf	shRNA,expressing
R15462	T21030	T21031	themeOf	luciferase,expression
R15463	T21032	T21033	themeOf	NFAT5,binding
R15464	T21035	T21036	themeOf	luciferase,expression

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