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PMC:3317373 / 7878-9366 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T3671 1479-1487 NN denotes standard
T3670 1470-1478 JJ denotes internal
T3669 1467-1469 IN denotes as
T3668 1462-1466 VB denotes used
T3667 1458-1461 VB denotes was
T3666 1450-1457 NN denotes β-actin
T3665 1447-1449 IN denotes of
T3664 1441-1446 NN denotes level
T3663 1437-1440 DT denotes The
T3662 1430-1435 NN denotes blots
T3661 1426-1429 DT denotes the
T3660 1423-1425 IN denotes of
T3659 1413-1422 NN denotes intensity
T3658 1409-1412 DT denotes the
T3657 1401-1408 VB denotes analyze
T3656 1398-1400 TO denotes to
T3655 1393-1397 VB denotes used
T3654 1389-1392 VB denotes was
T3653 1382-1388 NN denotes ImageJ
T3652 1379-1380 -RRB- denotes )
T3651 1374-1379 NNP denotes Japan
T3650 1372-1373 -COMMA- denotes ,
T3649 1367-1372 NNP denotes Tokyo
T3648 1365-1366 -COMMA- denotes ,
T3647 1356-1365 NNP denotes Hyperfilm
T3646 1351-1355 NNP denotes Fuji
T3645 1350-1351 -LRB- denotes (
T3644 1345-1349 NN denotes film
T3643 1332-1344 JJ denotes radiographic
T3642 1329-1331 TO denotes to
T3641 1321-1328 VB denotes exposed
T3640 1317-1320 CC denotes and
T3639 1315-1316 -RRB- denotes )
T3638 1313-1315 NNP denotes UK
T3637 1311-1312 -COMMA- denotes ,
T3636 1306-1311 NNP denotes Bucks
T3635 1304-1305 -COMMA- denotes ,
T3634 1293-1304 NNP denotes Biosciences
T3633 1284-1292 NNP denotes Amersham
T3632 1283-1284 -LRB- denotes (
T3631 1276-1282 NN denotes system
T3630 1266-1275 NN denotes detection
T3629 1264-1265 -RRB- denotes )
T3628 1261-1264 NN denotes ECL
T3627 1260-1261 -LRB- denotes (
T3626 1242-1259 NN denotes chemiluminescence
T3625 1233-1241 VB denotes enhanced
T3624 1228-1232 IN denotes with
T3623 1218-1227 VB denotes incubated
T3622 1213-1217 VB denotes were
T3621 1207-1212 NN denotes Blots
T3620 1204-1205 NN denotes h
T3619 1202-1203 CD denotes 1
T3618 1198-1201 IN denotes for
T3617 1189-1197 NN denotes dilution
T3616 1184-1188 CD denotes 2000
T3615 1182-1183 -COLON- denotes :
T3614 1180-1181 CD denotes 1
T3613 1178-1179 DT denotes a
T3612 1175-1177 IN denotes at
T3611 1166-1174 NN denotes antibody
T3610 1156-1165 JJ denotes secondary
T3609 1138-1155 JJ denotes peroxidase-linked
T3608 1126-1137 JJ denotes horseradish
T3607 1122-1125 DT denotes the
T3606 1117-1121 IN denotes with
T3605 1109-1116 VB denotes covered
T3604 1105-1108 CC denotes and
T3603 1098-1104 NN denotes buffer
T3602 1090-1097 VB denotes washing
T3601 1086-1089 DT denotes the
T3600 1083-1085 IN denotes in
T3599 1077-1082 NN denotes times
T3598 1071-1076 CD denotes three
T3597 1064-1070 VB denotes washed
T3596 1059-1063 VB denotes were
T3595 1053-1058 NN denotes blots
T3594 1049-1052 DT denotes The
T3593 1036-1047 NN denotes temperature
T3592 1031-1035 NN denotes room
T3591 1028-1030 IN denotes at
T3590 1026-1027 NN denotes h
T3589 1024-1025 CD denotes 1
T3588 1020-1023 IN denotes for
T3587 1014-1019 NN denotes blots
T3586 1010-1013 DT denotes the
T3585 1007-1009 TO denotes to
T3584 1001-1006 VB denotes added
T3583 996-1000 RB denotes then
T3582 992-995 CC denotes and
T3581 985-991 NN denotes buffer
T3580 976-984 VB denotes blocking
T3579 973-975 IN denotes in
T3578 959-972 RB denotes appropriately
T3577 951-958 VB denotes diluted
T3576 947-950 VB denotes was
T3575 938-946 NN denotes antibody
T3574 930-937 JJ denotes primary
T3573 925-929 DT denotes Each
T3572 922-923 -RRB- denotes )
T3571 919-922 NN denotes kDa
T3570 916-918 CD denotes 92
T3569 914-915 -COMMA- denotes ,
T3568 911-914 NNP denotes USA
T3567 909-910 -COMMA- denotes ,
T3566 907-909 NNP denotes CA
T3565 905-906 -COMMA- denotes ,
T3564 892-905 NNP denotes Biotechnology
T3563 887-891 NNP denotes Cruz
T3562 881-886 NNP denotes Santa
T3561 879-880 -COMMA- denotes ,
T3560 872-879 NN denotes sc-6841
T3559 871-872 -LRB- denotes (
T3558 869-870 -RRB- denotes )
T3557 865-869 NN denotes MMP9
T3556 864-865 -LRB- denotes (
T3555 862-863 CD denotes 9
T3554 845-861 NN denotes metallopeptidase
T3553 833-844 JJ denotes anti-matrix
T3552 829-832 CC denotes and
T3551 827-828 -RRB- denotes )
T3550 824-827 NN denotes kDa
T3549 821-823 CD denotes 43
T3548 819-820 -COMMA- denotes ,
T3547 816-819 NNP denotes USA
T3546 814-815 -COMMA- denotes ,
T3545 812-814 NNP denotes CA
T3544 810-811 -COMMA- denotes ,
T3543 797-810 NNP denotes Biotechnology
T3542 792-796 NNP denotes Cruz
T3541 786-791 NNP denotes Santa
T3540 784-785 -COMMA- denotes ,
T3539 775-784 NN denotes sc-130657
T3538 774-775 -LRB- denotes (
T3537 761-773 JJ denotes anti-β-actin
T3536 759-760 -COLON- denotes :
T3535 755-759 VB denotes used
T3534 750-754 VB denotes were
T3533 739-749 NN denotes antibodies
T3532 729-738 VB denotes following
T3531 725-728 DT denotes the
T3530 721-724 CC denotes and
T3529 719-720 -COMMA- denotes ,
T3528 716-719 NN denotes 4°C
T3527 713-715 IN denotes at
T3526 703-712 RB denotes overnight
T3525 696-702 NN denotes buffer
T3524 689-695 NN denotes saline
T3523 686-688 IN denotes in
T3522 681-685 NN denotes milk
T3521 677-680 JJ denotes dry
T3520 670-676 JJ denotes nonfat
T3519 668-669 NN denotes %
T3518 667-668 CD denotes 5
T3517 662-666 IN denotes with
T3516 654-661 VB denotes blocked
T3515 649-653 VB denotes were
T3514 639-648 NN denotes membranes
T3513 637-638 -COMMA- denotes ,
T3512 623-637 NN denotes immunoblotting
T3511 619-622 IN denotes For
T3510 609-617 NN denotes membrane
T3509 594-608 NN denotes nitrocellulose
T3508 592-593 DT denotes a
T3507 587-591 IN denotes onto
T3506 572-586 VB denotes electroblotted
T3505 568-571 CC denotes and
T3504 566-567 -COMMA- denotes ,
T3503 551-566 NN denotes electrophoresis
T3502 547-550 NN denotes gel
T3501 532-546 NN denotes polyacrylamide
T3500 524-531 NN denotes sulfate
T3499 516-523 NN denotes dodecyl
T3498 509-515 NN denotes sodium
T3497 507-508 NN denotes %
T3496 505-507 CD denotes 10
T3495 502-504 IN denotes by
T3494 492-501 VB denotes separated
T3493 490-491 -COMMA- denotes ,
T3492 484-490 NN denotes buffer
T3491 476-483 NN denotes loading
T3490 469-475 NN denotes sample
T3489 461-468 NNP denotes Laemmli
T3479 383-393 NN denotes micrograms
T3478 377-382 CD denotes Fifty
T3477 374-375 -RRB- denotes )
T3476 371-374 NNP denotes USA
T3475 369-370 -COMMA- denotes ,
T3474 367-369 NNP denotes IL
T3473 365-366 -COMMA- denotes ,
T3472 359-365 NNP denotes Pierce
T3471 358-359 -LRB- denotes (
T3470 350-357 NNP denotes Reagent
T3469 344-349 NNP denotes Assay
T3468 336-343 NNP denotes Protein
T3467 331-335 NNP denotes Plus
T3466 321-330 NNP denotes Coomassie
T3465 318-320 IN denotes by
T3464 308-317 VB denotes estimated
T3463 303-307 VB denotes were
T3462 288-302 NN denotes concentrations
T3461 280-287 NN denotes Protein
T3460 275-278 NN denotes 4°C
T3459 272-274 IN denotes at
T3458 268-271 NN denotes min
T3457 265-267 CD denotes 15
T3456 261-264 IN denotes for
T3455 259-260 NN denotes g
T3454 252-258 CD denotes 12,000
T3453 249-251 IN denotes at
T3452 237-248 VB denotes centrifuged
T3451 233-236 CC denotes and
T3450 231-232 -RRB- denotes )
T3449 228-231 CD denotes 7.4
T3448 225-227 NN denotes pH
T3447 221-224 NNP denotes PBS
T3446 218-220 IN denotes in
T3445 209-217 NN denotes cocktail
T3444 199-208 NN denotes inhibitor
T3443 190-198 NN denotes protease
T3442 186-189 CC denotes and
T3441 184-185 -COMMA- denotes ,
T3440 180-184 NN denotes PMSF
T3439 177-179 NN denotes mM
T3438 175-176 CD denotes 1
T3437 173-174 -COMMA- denotes ,
T3436 170-173 NN denotes DTT
T3435 167-169 NN denotes mM
T3434 163-166 CD denotes 0.5
T3433 161-162 -COMMA- denotes ,
T3432 158-161 NN denotes NaF
T3431 155-157 NN denotes mM
T3430 152-154 CD denotes 20
T3429 150-151 -COMMA- denotes ,
T3428 144-150 NN denotes Na3VO4
T3427 141-143 NN denotes mM
T3426 139-140 CD denotes 1
T3425 137-138 -COMMA- denotes ,
T3424 133-137 NN denotes EGTA
T3423 130-132 NN denotes mM
T3422 128-129 CD denotes 1
T3421 126-127 -COMMA- denotes ,
T3420 122-126 NN denotes EDTA
T3419 119-121 NN denotes mM
T3418 117-118 CD denotes 1
T3417 115-116 -COMMA- denotes ,
T3416 112-115 NN denotes SDS
T3415 110-111 NN denotes %
T3414 107-110 CD denotes 0.1
T3413 105-106 -COMMA- denotes ,
T3412 93-105 NN denotes deoxycholate
T3411 86-92 NN denotes sodium
T3410 84-85 NN denotes %
T3409 81-84 CD denotes 0.5
T3408 79-80 -COMMA- denotes ,
T3407 75-79 NN denotes NP40
T3406 73-74 NN denotes %
T3405 72-73 CD denotes 1
T3404 71-72 -LRB- denotes (
T3403 64-70 NN denotes buffer
T3402 59-63 NN denotes RIPA
T3401 56-58 IN denotes in
T3400 44-55 VB denotes homogenized
T3399 39-43 VB denotes were
T3398 33-38 NN denotes cells
T3397 31-32 -COMMA- denotes ,
T3396 24-31 NN denotes lysates
T3395 16-23 NN denotes protein
T3394 10-15 JJ denotes total
T3393 3-9 VB denotes obtain
T3392 0-2 TO denotes To
T3488 458-460 IN denotes in
T3487 443-457 JJ denotes heat-denatured
T3486 438-442 VB denotes were
T3485 429-437 NN denotes extracts
T3484 421-428 NN denotes protein
T3483 411-420 JJ denotes cytosolic
T3482 401-410 VB denotes resulting
T3481 397-400 DT denotes the
T3480 394-396 IN denotes of
R2900 T3393 T3392 arg1Of obtain,To
R2901 T3396 T3393 arg2Of lysates,obtain
R2902 T3396 T3394 arg1Of lysates,total
R2903 T3396 T3395 arg1Of lysates,protein
R2904 T3398 T3393 arg1Of cells,obtain
R2905 T3398 T3399 arg1Of cells,were
R2906 T3398 T3400 arg2Of cells,homogenized
R2907 T3398 T3452 arg2Of cells,centrifuged
R2908 T3400 T3401 arg1Of homogenized,in
R2909 T3400 T3451 arg1Of homogenized,and
R2910 T3403 T3401 arg2Of buffer,in
R2911 T3403 T3402 arg1Of buffer,RIPA
R2912 T3403 T3404 arg1Of buffer,(
R2913 T3405 T3406 arg1Of 1,%
R2914 T3407 T3405 arg1Of NP40,1
R2915 T3407 T3408 arg1Of NP40,","
R2916 T3408 T3413 arg1Of ",",","
R2917 T3409 T3410 arg1Of 0.5,%
R2918 T3412 T3408 arg2Of deoxycholate,","
R2919 T3412 T3409 arg1Of deoxycholate,0.5
R2920 T3412 T3411 arg1Of deoxycholate,sodium
R2921 T3413 T3417 arg1Of ",",","
R2922 T3414 T3415 arg1Of 0.1,%
R2923 T3416 T3413 arg2Of SDS,","
R2924 T3416 T3414 arg1Of SDS,0.1
R2925 T3417 T3421 arg1Of ",",","
R2926 T3418 T3419 arg1Of 1,mM
R2927 T3420 T3417 arg2Of EDTA,","
R2928 T3420 T3418 arg1Of EDTA,1
R2929 T3421 T3404 arg2Of ",",(
R2930 T3421 T3425 arg1Of ",",","
R2931 T3422 T3423 arg1Of 1,mM
R2932 T3424 T3421 arg2Of EGTA,","
R2933 T3424 T3422 arg1Of EGTA,1
R2934 T3426 T3427 arg1Of 1,mM
R2935 T3428 T3426 arg1Of Na3VO4,1
R2936 T3428 T3429 arg1Of Na3VO4,","
R2937 T3429 T3433 arg1Of ",",","
R2938 T3430 T3431 arg1Of 20,mM
R2939 T3432 T3429 arg2Of NaF,","
R2940 T3432 T3430 arg1Of NaF,20
R2941 T3433 T3437 arg1Of ",",","
R2942 T3434 T3435 arg1Of 0.5,mM
R2943 T3436 T3433 arg2Of DTT,","
R2944 T3436 T3434 arg1Of DTT,0.5
R2945 T3437 T3442 arg1Of ",",and
R2946 T3438 T3439 arg1Of 1,mM
R2947 T3440 T3437 arg2Of PMSF,","
R2948 T3440 T3438 arg1Of PMSF,1
R2949 T3442 T3425 arg2Of and,","
R2950 T3442 T3441 arg1Of and,","
R2951 T3445 T3442 arg2Of cocktail,and
R2952 T3445 T3443 arg1Of cocktail,protease
R2953 T3445 T3444 arg1Of cocktail,inhibitor
R2954 T3445 T3446 arg1Of cocktail,in
R2955 T3448 T3446 arg2Of pH,in
R2956 T3448 T3447 arg1Of pH,PBS
R2957 T3448 T3449 arg1Of pH,7.4
R2958 T3450 T3404 arg3Of ),(
R2959 T3451 T3392 modOf and,To
R2960 T3451 T3397 arg1Of and,","
R2961 T3451 T3399 arg2Of and,were
R2962 T3452 T3451 arg2Of centrifuged,and
R2963 T3452 T3453 arg1Of centrifuged,at
R2964 T3452 T3456 arg1Of centrifuged,for
R2965 T3455 T3453 arg2Of g,at
R2966 T3455 T3454 arg1Of g,"12,000"
R2967 T3458 T3456 arg2Of min,for
R2968 T3458 T3457 arg1Of min,15
R2969 T3458 T3459 arg1Of min,at
R2970 T3460 T3459 arg2Of 4°C,at
R2971 T3462 T3461 arg1Of concentrations,Protein
R2972 T3462 T3463 arg1Of concentrations,were
R2973 T3462 T3464 arg2Of concentrations,estimated
R2974 T3464 T3463 arg2Of estimated,were
R2975 T3470 T3464 arg1Of Reagent,estimated
R2976 T3470 T3465 arg2Of Reagent,by
R2977 T3470 T3466 arg1Of Reagent,Coomassie
R2978 T3470 T3467 arg1Of Reagent,Plus
R2979 T3470 T3468 arg1Of Reagent,Protein
R2980 T3470 T3469 arg1Of Reagent,Assay
R2981 T3470 T3471 arg1Of Reagent,(
R2982 T3472 T3473 arg1Of Pierce,","
R2983 T3473 T3471 arg2Of ",",(
R2984 T3473 T3475 arg1Of ",",","
R2985 T3474 T3473 arg2Of IL,","
R2986 T3476 T3475 arg2Of USA,","
R2987 T3477 T3471 arg3Of ),(
R2988 T3479 T3478 arg1Of micrograms,Fifty
R2989 T3479 T3480 arg1Of micrograms,of
R2990 T3479 T3486 arg1Of micrograms,were
R2991 T3479 T3487 arg1Of micrograms,heat-denatured
R2992 T3479 T3506 arg1Of micrograms,electroblotted
R2993 T3485 T3480 arg2Of extracts,of
R2994 T3485 T3481 arg1Of extracts,the
R2995 T3485 T3482 arg1Of extracts,resulting
R2996 T3485 T3483 arg1Of extracts,cytosolic
R2997 T3485 T3484 arg1Of extracts,protein
R2998 T3486 T3488 arg1Of were,in
R2999 T3486 T3505 arg1Of were,and
R3000 T3487 T3486 arg2Of heat-denatured,were
R3001 T3492 T3488 arg2Of buffer,in
R3002 T3492 T3489 arg1Of buffer,Laemmli
R3003 T3492 T3490 arg1Of buffer,sample
R3004 T3492 T3491 arg1Of buffer,loading
R3005 T3492 T3493 arg1Of buffer,","
R3006 T3492 T3494 arg2Of buffer,separated
R3007 T3496 T3497 arg1Of 10,%
R3008 T3503 T3494 arg1Of electrophoresis,separated
R3009 T3503 T3495 arg2Of electrophoresis,by
R3010 T3503 T3496 arg1Of electrophoresis,10
R3011 T3503 T3498 arg1Of electrophoresis,sodium
R3012 T3503 T3499 arg1Of electrophoresis,dodecyl
R3013 T3503 T3500 arg1Of electrophoresis,sulfate
R3014 T3503 T3501 arg1Of electrophoresis,polyacrylamide
R3015 T3503 T3502 arg1Of electrophoresis,gel
R3016 T3505 T3504 arg1Of and,","
R3017 T3506 T3505 arg2Of electroblotted,and
R3018 T3506 T3507 arg1Of electroblotted,onto
R3019 T3510 T3507 arg2Of membrane,onto
R3020 T3510 T3508 arg1Of membrane,a
R3021 T3510 T3509 arg1Of membrane,nitrocellulose
R3022 T3512 T3511 arg2Of immunoblotting,For
R3023 T3514 T3515 arg1Of membranes,were
R3024 T3514 T3516 arg2Of membranes,blocked
R3025 T3516 T3511 arg1Of blocked,For
R3026 T3516 T3513 arg1Of blocked,","
R3027 T3516 T3515 arg2Of blocked,were
R3028 T3516 T3517 arg1Of blocked,with
R3029 T3516 T3526 arg1Of blocked,overnight
R3030 T3516 T3527 arg1Of blocked,at
R3031 T3516 T3530 arg1Of blocked,and
R3032 T3518 T3519 arg1Of 5,%
R3033 T3522 T3517 arg2Of milk,with
R3034 T3522 T3518 arg1Of milk,5
R3035 T3522 T3520 arg1Of milk,nonfat
R3036 T3522 T3521 arg1Of milk,dry
R3037 T3522 T3523 arg1Of milk,in
R3038 T3525 T3523 arg2Of buffer,in
R3039 T3525 T3524 arg1Of buffer,saline
R3040 T3528 T3527 arg2Of 4°C,at
R3041 T3530 T3529 arg1Of and,","
R3042 T3530 T3536 arg1Of and,:
R3043 T3530 T3537 arg1Of and,anti-β-actin
R3044 T3530 T3554 arg1Of and,metallopeptidase
R3045 T3533 T3531 arg1Of antibodies,the
R3046 T3533 T3532 arg1Of antibodies,following
R3047 T3533 T3534 arg1Of antibodies,were
R3048 T3533 T3535 arg2Of antibodies,used
R3049 T3535 T3530 arg2Of used,and
R3050 T3535 T3534 arg2Of used,were
R3051 T3537 T3538 arg1Of anti-β-actin,(
R3052 T3537 T3552 arg1Of anti-β-actin,and
R3053 T3539 T3540 arg1Of sc-130657,","
R3054 T3539 T3544 arg1Of sc-130657,","
R3055 T3543 T3540 arg2Of Biotechnology,","
R3056 T3543 T3541 arg1Of Biotechnology,Santa
R3057 T3543 T3542 arg1Of Biotechnology,Cruz
R3058 T3544 T3538 arg2Of ",",(
R3059 T3545 T3546 arg1Of CA,","
R3060 T3546 T3548 arg1Of ",",","
R3061 T3547 T3546 arg2Of USA,","
R3062 T3549 T3548 arg2Of 43,","
R3063 T3550 T3544 arg2Of kDa,","
R3064 T3550 T3545 arg1Of kDa,CA
R3065 T3550 T3547 arg1Of kDa,USA
R3066 T3550 T3549 arg1Of kDa,43
R3067 T3551 T3538 arg3Of ),(
R3068 T3552 T3559 arg1Of and,(
R3069 T3554 T3552 arg2Of metallopeptidase,and
R3070 T3554 T3553 arg1Of metallopeptidase,anti-matrix
R3071 T3554 T3555 arg1Of metallopeptidase,9
R3072 T3554 T3556 arg1Of metallopeptidase,(
R3073 T3557 T3556 arg2Of MMP9,(
R3074 T3558 T3556 arg3Of ),(
R3075 T3560 T3561 arg1Of sc-6841,","
R3076 T3560 T3565 arg1Of sc-6841,","
R3077 T3564 T3561 arg2Of Biotechnology,","
R3078 T3564 T3562 arg1Of Biotechnology,Santa
R3079 T3564 T3563 arg1Of Biotechnology,Cruz
R3080 T3565 T3559 arg2Of ",",(
R3081 T3566 T3567 arg1Of CA,","
R3082 T3567 T3569 arg1Of ",",","
R3083 T3568 T3567 arg2Of USA,","
R3084 T3570 T3569 arg2Of 92,","
R3085 T3571 T3565 arg2Of kDa,","
R3086 T3571 T3566 arg1Of kDa,CA
R3087 T3571 T3568 arg1Of kDa,USA
R3088 T3571 T3570 arg1Of kDa,92
R3089 T3572 T3559 arg3Of ),(
R3090 T3575 T3573 arg1Of antibody,Each
R3091 T3575 T3574 arg1Of antibody,primary
R3092 T3575 T3576 arg1Of antibody,was
R3093 T3575 T3577 arg2Of antibody,diluted
R3094 T3575 T3584 arg1Of antibody,added
R3095 T3577 T3576 arg2Of diluted,was
R3096 T3577 T3578 arg1Of diluted,appropriately
R3097 T3577 T3579 arg1Of diluted,in
R3098 T3577 T3582 arg1Of diluted,and
R3099 T3580 T3579 arg2Of blocking,in
R3100 T3581 T3580 arg2Of buffer,blocking
R3101 T3584 T3582 arg2Of added,and
R3102 T3584 T3583 arg1Of added,then
R3103 T3584 T3585 arg1Of added,to
R3104 T3584 T3588 arg1Of added,for
R3105 T3587 T3585 arg2Of blots,to
R3106 T3587 T3586 arg1Of blots,the
R3107 T3590 T3588 arg2Of h,for
R3108 T3590 T3589 arg1Of h,1
R3109 T3590 T3591 arg1Of h,at
R3110 T3593 T3591 arg2Of temperature,at
R3111 T3593 T3592 arg1Of temperature,room
R3112 T3595 T3594 arg1Of blots,The
R3113 T3595 T3596 arg1Of blots,were
R3114 T3595 T3597 arg2Of blots,washed
R3115 T3595 T3605 arg2Of blots,covered
R3116 T3597 T3599 arg1Of washed,times
R3117 T3597 T3600 arg1Of washed,in
R3118 T3597 T3604 arg1Of washed,and
R3119 T3599 T3598 arg1Of times,three
R3120 T3603 T3600 arg2Of buffer,in
R3121 T3603 T3601 arg1Of buffer,the
R3122 T3603 T3602 arg1Of buffer,washing
R3123 T3604 T3596 arg2Of and,were
R3124 T3605 T3604 arg2Of covered,and
R3125 T3605 T3606 arg1Of covered,with
R3126 T3605 T3612 arg1Of covered,at
R3127 T3611 T3606 arg2Of antibody,with
R3128 T3611 T3607 arg1Of antibody,the
R3129 T3611 T3608 arg1Of antibody,horseradish
R3130 T3611 T3609 arg1Of antibody,peroxidase-linked
R3131 T3611 T3610 arg1Of antibody,secondary
R3132 T3614 T3612 arg2Of 1,at
R3133 T3614 T3613 arg1Of 1,a
R3134 T3614 T3615 arg1Of 1,:
R3135 T3617 T3616 arg1Of dilution,2000
R3136 T3617 T3618 arg1Of dilution,for
R3137 T3620 T3618 arg2Of h,for
R3138 T3620 T3619 arg1Of h,1
R3139 T3621 T3622 arg1Of Blots,were
R3140 T3621 T3623 arg2Of Blots,incubated
R3141 T3621 T3641 arg2Of Blots,exposed
R3142 T3623 T3624 arg1Of incubated,with
R3143 T3623 T3640 arg1Of incubated,and
R3144 T3628 T3627 arg2Of ECL,(
R3145 T3629 T3627 arg3Of ),(
R3146 T3631 T3624 arg2Of system,with
R3147 T3631 T3625 arg2Of system,enhanced
R3148 T3631 T3626 arg1Of system,chemiluminescence
R3149 T3631 T3627 arg1Of system,(
R3150 T3631 T3630 arg1Of system,detection
R3151 T3631 T3632 arg1Of system,(
R3152 T3634 T3632 arg2Of Biosciences,(
R3153 T3634 T3633 arg1Of Biosciences,Amersham
R3154 T3634 T3635 arg1Of Biosciences,","
R3155 T3636 T3635 arg2Of Bucks,","
R3156 T3636 T3637 arg1Of Bucks,","
R3157 T3638 T3637 arg2Of UK,","
R3158 T3639 T3632 arg3Of ),(
R3159 T3640 T3622 arg2Of and,were
R3160 T3641 T3640 arg2Of exposed,and
R3161 T3641 T3642 arg1Of exposed,to
R3162 T3644 T3642 arg2Of film,to
R3163 T3644 T3643 arg1Of film,radiographic
R3164 T3644 T3645 arg1Of film,(
R3165 T3647 T3645 arg2Of Hyperfilm,(
R3166 T3647 T3646 arg1Of Hyperfilm,Fuji
R3167 T3647 T3648 arg1Of Hyperfilm,","
R3168 T3649 T3648 arg2Of Tokyo,","
R3169 T3649 T3650 arg1Of Tokyo,","
R3170 T3651 T3650 arg2Of Japan,","
R3171 T3652 T3645 arg3Of ),(
R3172 T3653 T3654 arg1Of ImageJ,was
R3173 T3653 T3655 arg2Of ImageJ,used
R3174 T3655 T3654 arg2Of used,was
R3175 T3657 T3655 arg3Of analyze,used
R3176 T3657 T3656 arg1Of analyze,to
R3177 T3659 T3657 arg2Of intensity,analyze
R3178 T3659 T3658 arg1Of intensity,the
R3179 T3659 T3660 arg1Of intensity,of
R3180 T3662 T3660 arg2Of blots,of
R3181 T3662 T3661 arg1Of blots,the
R3182 T3664 T3663 arg1Of level,The
R3183 T3664 T3665 arg1Of level,of
R3184 T3664 T3667 arg1Of level,was
R3185 T3664 T3668 arg2Of level,used
R3186 T3666 T3665 arg2Of β-actin,of
R3187 T3668 T3667 arg2Of used,was
R3188 T3668 T3669 arg1Of used,as
R3189 T3671 T3669 arg2Of standard,as
R3190 T3671 T3670 arg1Of standard,internal

bionlp-st-ge-2016-test-proteins

Id Subject Object Predicate Lexical cue
T3385 865-869 Protein denotes MMP9

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T3688 1450-1457 http://www.uniprot.org/uniprot/P60709 denotes β-actin
T3687 766-773 http://www.uniprot.org/uniprot/P60709 denotes β-actin

UBERON-AE

Id Subject Object Predicate Lexical cue
T3373 681-685 http://purl.obolibrary.org/obo/UBERON_0001913 denotes milk

GO-BP

Id Subject Object Predicate Lexical cue
T3388 907-909 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T3387 812-814 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T3386 524-531 http://purl.obolibrary.org/obo/GO_0051923 denotes sulfate

GO-MF

Id Subject Object Predicate Lexical cue
T3674 1166-1174 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T3673 938-946 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T3672 739-749 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T3676 907-909 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T3675 812-814 http://purl.obolibrary.org/obo/GO_0033968 denotes CA

GO-CC

Id Subject Object Predicate Lexical cue
T3685 1166-1174 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T3684 938-946 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T3683 739-749 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T3682 1166-1174 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T3681 938-946 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T3680 739-749 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T3679 639-648 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T3678 609-617 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T3677 33-38 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

sentences

Id Subject Object Predicate Lexical cue
T3383 1437-1488 Sentence denotes The level of β-actin was used as internal standard.
T3382 1382-1436 Sentence denotes ImageJ was used to analyze the intensity of the blots.
T3381 1207-1381 Sentence denotes Blots were incubated with enhanced chemiluminescence (ECL) detection system (Amersham Biosciences, Bucks, UK) and exposed to radiographic film (Fuji Hyperfilm, Tokyo, Japan).
T3380 1049-1206 Sentence denotes The blots were washed three times in the washing buffer and covered with the horseradish peroxidase-linked secondary antibody at a 1 : 2000 dilution for 1 h.
T3379 925-1048 Sentence denotes Each primary antibody was diluted appropriately in blocking buffer and then added to the blots for 1 h at room temperature.
T3378 619-924 Sentence denotes For immunoblotting, membranes were blocked with 5% nonfat dry milk in saline buffer overnight at 4°C, and the following antibodies were used: anti-β-actin (sc-130657, Santa Cruz Biotechnology, CA, USA, 43 kDa) and anti-matrix metallopeptidase 9 (MMP9) (sc-6841, Santa Cruz Biotechnology, CA, USA, 92 kDa).
T3377 377-618 Sentence denotes Fifty micrograms of the resulting cytosolic protein extracts were heat-denatured in Laemmli sample loading buffer, separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis, and electroblotted onto a nitrocellulose membrane.
T3376 0-376 Sentence denotes To obtain total protein lysates, cells were homogenized in RIPA buffer (1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, 20 mM NaF, 0.5 mM DTT, 1 mM PMSF, and protease inhibitor cocktail in PBS pH 7.4) and centrifuged at 12,000 g for 15 min at 4°C. Protein concentrations were estimated by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA).
T85 0-279 Sentence denotes To obtain total protein lysates, cells were homogenized in RIPA buffer (1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, 20 mM NaF, 0.5 mM DTT, 1 mM PMSF, and protease inhibitor cocktail in PBS pH 7.4) and centrifuged at 12,000 g for 15 min at 4°C.
T86 280-376 Sentence denotes Protein concentrations were estimated by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA).
T87 377-618 Sentence denotes Fifty micrograms of the resulting cytosolic protein extracts were heat-denatured in Laemmli sample loading buffer, separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis, and electroblotted onto a nitrocellulose membrane.
T88 619-924 Sentence denotes For immunoblotting, membranes were blocked with 5% nonfat dry milk in saline buffer overnight at 4°C, and the following antibodies were used: anti-β-actin (sc-130657, Santa Cruz Biotechnology, CA, USA, 43 kDa) and anti-matrix metallopeptidase 9 (MMP9) (sc-6841, Santa Cruz Biotechnology, CA, USA, 92 kDa).
T89 925-1048 Sentence denotes Each primary antibody was diluted appropriately in blocking buffer and then added to the blots for 1 h at room temperature.
T90 1049-1206 Sentence denotes The blots were washed three times in the washing buffer and covered with the horseradish peroxidase-linked secondary antibody at a 1 : 2000 dilution for 1 h.
T91 1207-1381 Sentence denotes Blots were incubated with enhanced chemiluminescence (ECL) detection system (Amersham Biosciences, Bucks, UK) and exposed to radiographic film (Fuji Hyperfilm, Tokyo, Japan).
T92 1382-1436 Sentence denotes ImageJ was used to analyze the intensity of the blots.
T93 1437-1488 Sentence denotes The level of β-actin was used as internal standard.

simple1

Id Subject Object Predicate Lexical cue
T3686 865-869 Protein denotes MMP9

BioNLP16_DUT

Id Subject Object Predicate Lexical cue
T3997 865-869 Protein denotes MMP9

BioNLP16_Messiy

Id Subject Object Predicate Lexical cue
T3690 865-869 Protein denotes MMP9

DLUT931

Id Subject Object Predicate Lexical cue
T3689 865-869 Protein denotes MMP9

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T4033 1306-1315 Protein denotes Bucks, UK
T4032 359-369 Entity denotes Pierce, IL
T4031 277-287 Protein denotes C. Protein
T4030 1261-1264 Protein denotes ECL
T4029 1450-1457 Protein denotes β-actin
T4028 429-437 Entity denotes extracts
T4027 689-695 Entity denotes saline
T4026 594-617 Entity denotes nitrocellulose membrane
T4025 152-161 Entity denotes 20 mM NaF
T4024 725-749 Entity denotes the following antibodies
T4023 117-138 Entity denotes 1 mM EDTA, 1 mM EGTA,
T4022 175-184 Entity denotes 1 mM PMSF
T4021 865-869 Protein denotes MMP9
T4020 833-870 Protein denotes anti-matrix metallopeptidase 9 (MMP9)
T4019 594-608 Entity denotes nitrocellulose
T4018 190-198 Protein denotes protease
T4017 421-428 Protein denotes protein
T4016 502-566 Entity denotes by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis
T4015 930-946 Protein denotes primary antibody
T4014 33-38 Entity denotes cells
T4013 10-32 Protein denotes total protein lysates,
T4012 107-115 Protein denotes 0.1% SDS
T4011 1156-1174 Protein denotes secondary antibody
T4010 350-370 Entity denotes Reagent (Pierce, IL,
T4009 502-566 Entity denotes by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis
T4008 81-116 Entity denotes 0.5% sodium deoxycholate, 0.1% SDS,
T4007 755-785 Protein denotes used: anti-β-actin (sc-130657,
T4006 639-648 Entity denotes membranes
T4005 336-343 Entity denotes Protein
T4004 1109-1148 Protein denotes covered with the horseradish peroxidase
T4003 532-546 Entity denotes polyacrylamide
T4002 190-217 Entity denotes protease inhibitor cocktail
T4001 881-886 Entity denotes Santa
T4000 786-810 Entity denotes Santa Cruz Biotechnology
T3999 128-137 Entity denotes 1 mM EGTA
T3998 163-173 Entity denotes 0.5 mM DTT

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T3991 1487-1488 . denotes .
T3990 1479-1487 NN denotes standard
T3989 1470-1478 JJ denotes internal
T3988 1467-1469 IN denotes as
T3987 1462-1466 VBN denotes used
T3986 1458-1461 VBD denotes was
T3985 1450-1457 NN denotes β-actin
T3984 1447-1449 IN denotes of
T3983 1441-1446 NN denotes level
T3982 1437-1440 DT denotes The
T3981 1435-1436 . denotes .
T3980 1430-1435 NNS denotes blots
T3979 1426-1429 DT denotes the
T3978 1423-1425 IN denotes of
T3977 1413-1422 NN denotes intensity
T3976 1409-1412 DT denotes the
T3975 1401-1408 VB denotes analyze
T3974 1398-1400 TO denotes to
T3973 1393-1397 VBN denotes used
T3972 1389-1392 VBD denotes was
T3971 1382-1388 NNP denotes ImageJ
T3970 1380-1381 . denotes .
T3969 1379-1380 -RRB- denotes )
T3704 16-23 NN denotes protein
T3703 10-15 JJ denotes total
T3702 3-9 VB denotes obtain
T3701 0-2 TO denotes To
T3894 959-972 RB denotes appropriately
T3893 951-958 VBN denotes diluted
T3892 947-950 VBD denotes was
T3891 938-946 NN denotes antibody
T3865 824-827 NN denotes kDa
T3864 821-823 CD denotes 43
T3863 819-820 , denotes ,
T3862 816-819 NNP denotes USA
T3861 814-815 , denotes ,
T3860 812-814 NNP denotes CA
T3859 810-811 , denotes ,
T3858 797-810 NNP denotes Biotechnology
T3857 792-796 NNP denotes Cruz
T3856 786-791 NNP denotes Santa
T3855 784-785 , denotes ,
T3854 775-784 JJ denotes sc-130657
T3853 774-775 -LRB- denotes (
T3852 761-773 JJ denotes anti-β-actin
T3851 759-760 : denotes :
T3850 755-759 VBN denotes used
T3849 750-754 VBD denotes were
T3848 739-749 NNS denotes antibodies
T3847 729-738 VBG denotes following
T3846 725-728 DT denotes the
T3845 721-724 CC denotes and
T3844 719-720 , denotes ,
T3843 718-719 NNP denotes C
T3842 717-718 CD denotes °
T3841 716-717 CD denotes 4
T3840 713-715 IN denotes at
T3839 703-712 JJ denotes overnight
T3838 696-702 NN denotes buffer
T3837 689-695 NN denotes saline
T3836 686-688 IN denotes in
T3835 681-685 NN denotes milk
T3834 677-680 JJ denotes dry
T3833 670-676 JJ denotes nonfat
T3832 668-669 NN denotes %
T3831 667-668 CD denotes 5
T3830 662-666 IN denotes with
T3829 654-661 VBN denotes blocked
T3828 649-653 VBD denotes were
T3827 639-648 NNS denotes membranes
T3826 637-638 , denotes ,
T3825 623-637 VBG denotes immunoblotting
T3824 619-622 IN denotes For
T3823 617-618 . denotes .
T3822 609-617 NN denotes membrane
T3821 594-608 JJ denotes nitrocellulose
T3820 592-593 DT denotes a
T3819 587-591 IN denotes onto
T3818 572-586 VBD denotes electroblotted
T3817 568-571 CC denotes and
T3816 566-567 , denotes ,
T3815 551-566 NN denotes electrophoresis
T3814 547-550 NN denotes gel
T3813 532-546 NN denotes polyacrylamide
T3812 524-531 NN denotes sulfate
T3811 516-523 NN denotes dodecyl
T3810 509-515 NN denotes sodium
T3809 507-508 NN denotes %
T3808 505-507 CD denotes 10
T3787 371-374 NNP denotes USA
T3786 369-370 , denotes ,
T3785 367-369 NNP denotes IL
T3784 365-366 , denotes ,
T3783 359-365 NNP denotes Pierce
T3782 358-359 -LRB- denotes (
T3781 350-357 NNP denotes Reagent
T3780 344-349 NNP denotes Assay
T3779 336-343 NNP denotes Protein
T3778 331-335 NNP denotes Plus
T3777 321-330 NNP denotes Coomassie
T3776 318-320 IN denotes by
T3775 308-317 VBN denotes estimated
T3774 303-307 VBD denotes were
T3773 288-302 NNS denotes concentrations
T3772 280-287 NNP denotes Protein
T3771 277-279 NNP denotes C.
T3770 276-277 CD denotes °
T3769 275-276 CD denotes 4
T3768 272-274 IN denotes at
T3767 268-271 NN denotes min
T3766 265-267 CD denotes 15
T3765 261-264 IN denotes for
T3764 259-260 NN denotes g
T3763 252-258 CD denotes 12,000
T3762 249-251 IN denotes at
T3761 237-248 VBN denotes centrifuged
T3760 233-236 CC denotes and
T3759 231-232 -RRB- denotes )
T3758 228-231 CD denotes 7.4
T3757 225-227 NNP denotes pH
T3756 221-224 NNP denotes PBS
T3755 218-220 IN denotes in
T3754 209-217 NN denotes cocktail
T3753 199-208 NN denotes inhibitor
T3752 190-198 NN denotes protease
T3751 186-189 CC denotes and
T3750 184-185 , denotes ,
T3749 180-184 NNP denotes PMSF
T3748 177-179 NNP denotes mM
T3747 175-176 CD denotes 1
T3746 173-174 , denotes ,
T3745 170-173 NNP denotes DTT
T3744 167-169 NNP denotes mM
T3743 163-166 CD denotes 0.5
T3742 161-162 , denotes ,
T3741 158-161 NNP denotes NaF
T3740 155-157 NNP denotes mM
T3739 152-154 CD denotes 20
T3738 150-151 , denotes ,
T3737 144-150 NNP denotes Na3VO4
T3736 141-143 NNP denotes mM
T3735 139-140 CD denotes 1
T3734 137-138 , denotes ,
T3733 133-137 NNP denotes EGTA
T3732 130-132 NNP denotes mM
T3731 128-129 CD denotes 1
T3730 126-127 , denotes ,
T3729 122-126 NNP denotes EDTA
T3728 119-121 NNP denotes mM
T3727 117-118 CD denotes 1
T3726 115-116 , denotes ,
T3725 112-115 NNP denotes SDS
T3724 110-111 NN denotes %
T3723 107-110 CD denotes 0.1
T3722 105-106 , denotes ,
T3721 93-105 NN denotes deoxycholate
T3720 86-92 NN denotes sodium
T3719 84-85 NN denotes %
T3718 81-84 CD denotes 0.5
T3717 79-80 , denotes ,
T3716 75-79 CD denotes NP40
T3715 73-74 NN denotes %
T3714 72-73 CD denotes 1
T3713 71-72 -LRB- denotes (
T3712 64-70 NN denotes buffer
T3711 59-63 NNP denotes RIPA
T3710 56-58 IN denotes in
T3709 44-55 VBN denotes homogenized
T3708 39-43 VBD denotes were
T3707 33-38 NNS denotes cells
T3706 31-32 , denotes ,
T3705 24-31 VBZ denotes lysates
T3968 1374-1379 NNP denotes Japan
T3967 1372-1373 , denotes ,
T3966 1367-1372 NNP denotes Tokyo
T3965 1365-1366 , denotes ,
T3964 1356-1365 NNP denotes Hyperfilm
T3963 1351-1355 NNP denotes Fuji
T3962 1350-1351 -LRB- denotes (
T3961 1345-1349 NN denotes film
T3960 1332-1344 JJ denotes radiographic
T3959 1329-1331 TO denotes to
T3958 1321-1328 VBN denotes exposed
T3957 1317-1320 CC denotes and
T3956 1315-1316 -RRB- denotes )
T3955 1313-1315 NNP denotes UK
T3954 1311-1312 , denotes ,
T3953 1306-1311 NNP denotes Bucks
T3952 1304-1305 , denotes ,
T3951 1293-1304 NNP denotes Biosciences
T3950 1284-1292 NNP denotes Amersham
T3949 1283-1284 -LRB- denotes (
T3948 1276-1282 NN denotes system
T3947 1266-1275 NN denotes detection
T3946 1264-1265 -RRB- denotes )
T3945 1261-1264 NNP denotes ECL
T3944 1260-1261 -LRB- denotes (
T3943 1242-1259 NN denotes chemiluminescence
T3942 1233-1241 JJ denotes enhanced
T3941 1228-1232 IN denotes with
T3940 1218-1227 VBN denotes incubated
T3939 1213-1217 VBD denotes were
T3938 1207-1212 NNS denotes Blots
T3937 1204-1206 NN denotes h.
T3936 1202-1203 CD denotes 1
T3935 1198-1201 IN denotes for
T3934 1189-1197 NN denotes dilution
T3933 1184-1188 CD denotes 2000
T3932 1182-1183 : denotes :
T3931 1180-1181 CD denotes 1
T3930 1178-1179 DT denotes a
T3929 1175-1177 IN denotes at
T3928 1166-1174 NN denotes antibody
T3927 1156-1165 JJ denotes secondary
T3926 1138-1155 JJ denotes peroxidase-linked
T3925 1126-1137 JJ denotes horseradish
T3924 1122-1125 DT denotes the
T3923 1117-1121 IN denotes with
T3922 1109-1116 VBN denotes covered
T3921 1105-1108 CC denotes and
T3920 1098-1104 NN denotes buffer
T3919 1090-1097 VBG denotes washing
T3918 1086-1089 DT denotes the
T3917 1083-1085 IN denotes in
T3916 1077-1082 NNS denotes times
T3915 1071-1076 CD denotes three
T3914 1064-1070 VBN denotes washed
T3913 1059-1063 VBD denotes were
T3912 1053-1058 NNS denotes blots
T3911 1049-1052 DT denotes The
T3910 1047-1048 . denotes .
T3909 1036-1047 NN denotes temperature
T3908 1031-1035 NN denotes room
T3907 1028-1030 IN denotes at
T3906 1026-1027 NN denotes h
T3905 1024-1025 CD denotes 1
T3904 1020-1023 IN denotes for
T3903 1014-1019 NNS denotes blots
T3902 1010-1013 DT denotes the
T3901 1007-1009 TO denotes to
T3900 1001-1006 VBD denotes added
T3899 996-1000 RB denotes then
T3898 992-995 CC denotes and
T3897 985-991 NN denotes buffer
T3896 976-984 VBG denotes blocking
T3895 973-975 IN denotes in
T3800 458-460 IN denotes in
T3799 443-457 JJ denotes heat-denatured
T3798 438-442 VBD denotes were
T3797 429-437 NNS denotes extracts
T3796 421-428 NN denotes protein
T3795 411-420 JJ denotes cytosolic
T3794 401-410 VBG denotes resulting
T3793 397-400 DT denotes the
T3792 394-396 IN denotes of
T3791 383-393 NNS denotes micrograms
T3790 377-382 NNP denotes Fifty
T3789 375-376 . denotes .
T3788 374-375 -RRB- denotes )
T3807 502-504 IN denotes by
T3806 492-501 VBN denotes separated
T3805 490-491 , denotes ,
T3804 484-490 NN denotes buffer
T3803 476-483 VBG denotes loading
T3802 469-475 NN denotes sample
T3801 461-468 NNP denotes Laemmli
T3890 930-937 JJ denotes primary
T3889 925-929 DT denotes Each
T3888 923-924 . denotes .
T3887 922-923 -RRB- denotes )
T3886 919-922 NN denotes kDa
T3885 916-918 CD denotes 92
T3884 914-915 , denotes ,
T3883 911-914 NNP denotes USA
T3882 909-910 , denotes ,
T3881 907-909 NNP denotes CA
T3880 905-906 , denotes ,
T3879 892-905 NNP denotes Biotechnology
T3878 887-891 NNP denotes Cruz
T3877 881-886 NNP denotes Santa
T3876 879-880 , denotes ,
T3875 872-879 JJ denotes sc-6841
T3874 871-872 -LRB- denotes (
T3873 869-870 -RRB- denotes )
T3872 865-869 NNP denotes MMP9
T3871 864-865 -LRB- denotes (
T3870 862-863 CD denotes 9
T3869 845-861 NN denotes metallopeptidase
T3868 833-844 JJ denotes anti-matrix
T3867 829-832 CC denotes and
T3866 827-828 -RRB- denotes )
R3195 T3701 T3702 aux To,obtain
R3196 T3702 T3702 ROOT obtain,obtain
R3197 T3703 T3704 amod total,protein
R3198 T3704 T3702 dobj protein,obtain
R3199 T3705 T3709 advmod lysates,homogenized
R3200 T3706 T3709 punct ",",homogenized
R3201 T3707 T3709 nsubjpass cells,homogenized
R3202 T3708 T3709 auxpass were,homogenized
R3203 T3709 T3702 advcl homogenized,obtain
R3204 T3710 T3709 prep in,homogenized
R3205 T3711 T3712 compound RIPA,buffer
R3206 T3712 T3710 pobj buffer,in
R3207 T3713 T3715 punct (,%
R3208 T3714 T3715 nummod 1,%
R3209 T3715 T3775 nsubjpass %,estimated
R3210 T3716 T3721 nummod NP40,deoxycholate
R3211 T3717 T3716 punct ",",NP40
R3212 T3718 T3719 nummod 0.5,%
R3213 T3719 T3721 nmod %,deoxycholate
R3214 T3720 T3721 compound sodium,deoxycholate
R3215 T3721 T3715 appos deoxycholate,%
R3216 T3722 T3721 punct ",",deoxycholate
R3217 T3723 T3724 nummod 0.1,%
R3218 T3724 T3715 appos %,%
R3219 T3725 T3715 npadvmod SDS,%
R3220 T3726 T3725 punct ",",SDS
R3221 T3727 T3729 nummod 1,EDTA
R3222 T3728 T3729 compound mM,EDTA
R3223 T3729 T3725 appos EDTA,SDS
R3224 T3730 T3725 punct ",",SDS
R3225 T3731 T3733 nummod 1,EGTA
R3226 T3732 T3733 compound mM,EGTA
R3227 T3733 T3725 appos EGTA,SDS
R3228 T3734 T3725 punct ",",SDS
R3229 T3735 T3737 nummod 1,Na3VO4
R3230 T3736 T3737 compound mM,Na3VO4
R3231 T3737 T3725 appos Na3VO4,SDS
R3232 T3738 T3725 punct ",",SDS
R3233 T3739 T3741 nummod 20,NaF
R3234 T3740 T3741 compound mM,NaF
R3235 T3741 T3725 appos NaF,SDS
R3236 T3742 T3725 punct ",",SDS
R3237 T3743 T3745 nummod 0.5,DTT
R3238 T3744 T3745 compound mM,DTT
R3239 T3745 T3725 appos DTT,SDS
R3240 T3746 T3725 punct ",",SDS
R3241 T3747 T3749 nummod 1,PMSF
R3242 T3748 T3749 compound mM,PMSF
R3243 T3749 T3725 appos PMSF,SDS
R3244 T3750 T3725 punct ",",SDS
R3245 T3751 T3715 cc and,%
R3246 T3752 T3753 compound protease,inhibitor
R3247 T3753 T3754 compound inhibitor,cocktail
R3248 T3754 T3715 conj cocktail,%
R3297 T3803 T3804 compound loading,buffer
R3298 T3804 T3800 pobj buffer,in
R3299 T3805 T3799 punct ",",heat-denatured
R3300 T3806 T3798 advcl separated,were
R3301 T3807 T3806 agent by,separated
R3302 T3808 T3809 nummod 10,%
R3303 T3809 T3815 compound %,electrophoresis
R3304 T3810 T3812 compound sodium,sulfate
R3305 T3811 T3812 compound dodecyl,sulfate
R3306 T3812 T3814 compound sulfate,gel
R3307 T3813 T3814 compound polyacrylamide,gel
R3308 T3814 T3815 compound gel,electrophoresis
R3309 T3815 T3807 pobj electrophoresis,by
R3310 T3816 T3806 punct ",",separated
R3311 T3817 T3806 cc and,separated
R3312 T3818 T3806 conj electroblotted,separated
R3313 T3819 T3818 prep onto,electroblotted
R3314 T3820 T3822 det a,membrane
R3315 T3821 T3822 amod nitrocellulose,membrane
R3316 T3822 T3819 pobj membrane,onto
R3317 T3823 T3798 punct .,were
R3318 T3824 T3829 prep For,blocked
R3319 T3825 T3824 pobj immunoblotting,For
R3320 T3826 T3829 punct ",",blocked
R3321 T3827 T3829 nsubjpass membranes,blocked
R3322 T3828 T3829 auxpass were,blocked
R3323 T3829 T3829 ROOT blocked,blocked
R3324 T3830 T3829 prep with,blocked
R3325 T3831 T3832 nummod 5,%
R3326 T3832 T3835 nmod %,milk
R3327 T3833 T3835 amod nonfat,milk
R3328 T3834 T3835 amod dry,milk
R3329 T3835 T3830 pobj milk,with
R3330 T3836 T3835 prep in,milk
R3331 T3837 T3838 compound saline,buffer
R3332 T3838 T3839 compound buffer,overnight
R3333 T3839 T3836 pobj overnight,in
R3355 T3861 T3860 punct ",",CA
R3356 T3862 T3860 conj USA,CA
R3357 T3863 T3860 punct ",",CA
R3358 T3864 T3865 nummod 43,kDa
R3359 T3865 T3860 appos kDa,CA
R3360 T3866 T3865 punct ),kDa
R3361 T3867 T3865 cc and,kDa
R3362 T3868 T3869 amod anti-matrix,metallopeptidase
R3363 T3869 T3865 conj metallopeptidase,kDa
R3364 T3870 T3869 nummod 9,metallopeptidase
R3365 T3871 T3872 punct (,MMP9
R3366 T3872 T3869 appos MMP9,metallopeptidase
R3367 T3873 T3872 punct ),MMP9
R3368 T3874 T3875 punct (,sc-6841
R3369 T3875 T3869 appos sc-6841,metallopeptidase
R3370 T3876 T3875 punct ",",sc-6841
R3371 T3877 T3879 compound Santa,Biotechnology
R3372 T3878 T3879 compound Cruz,Biotechnology
R3373 T3879 T3875 appos Biotechnology,sc-6841
R3374 T3880 T3879 punct ",",Biotechnology
R3375 T3881 T3879 conj CA,Biotechnology
R3376 T3882 T3881 punct ",",CA
R3377 T3883 T3881 conj USA,CA
R3378 T3884 T3881 punct ",",CA
R3379 T3885 T3886 nummod 92,kDa
R3380 T3886 T3881 appos kDa,CA
R3381 T3887 T3875 punct ),sc-6841
R3382 T3888 T3854 punct .,sc-130657
R3383 T3889 T3891 det Each,antibody
R3384 T3890 T3891 amod primary,antibody
R3385 T3891 T3893 nsubjpass antibody,diluted
R3386 T3892 T3893 auxpass was,diluted
R3387 T3893 T3893 ROOT diluted,diluted
R3388 T3894 T3893 advmod appropriately,diluted
R3389 T3895 T3893 prep in,diluted
R3390 T3896 T3895 pcomp blocking,in
R3391 T3897 T3896 dobj buffer,blocking
R3392 T3898 T3893 cc and,diluted
R3393 T3899 T3900 advmod then,added
R3394 T3900 T3893 conj added,diluted
R3395 T3901 T3900 prep to,added
R3396 T3902 T3903 det the,blots
R3397 T3903 T3901 pobj blots,to
R3398 T3904 T3900 prep for,added
R3399 T3905 T3906 nummod 1,h
R3400 T3906 T3904 pobj h,for
R3401 T3907 T3906 prep at,h
R3402 T3908 T3909 compound room,temperature
R3403 T3909 T3907 pobj temperature,at
R3404 T3910 T3900 punct .,added
R3405 T3911 T3912 det The,blots
R3406 T3912 T3914 nsubjpass blots,washed
R3407 T3913 T3914 auxpass were,washed
R3408 T3914 T3914 ROOT washed,washed
R3409 T3915 T3916 nummod three,times
R3410 T3916 T3914 npadvmod times,washed
R3433 T3939 T3940 auxpass were,incubated
R3434 T3940 T3914 conj incubated,washed
R3435 T3941 T3940 prep with,incubated
R3436 T3942 T3943 amod enhanced,chemiluminescence
R3437 T3943 T3941 pobj chemiluminescence,with
R3438 T3944 T3945 punct (,ECL
R3439 T3945 T3943 appos ECL,chemiluminescence
R3440 T3946 T3945 punct ),ECL
R3441 T3947 T3948 compound detection,system
R3442 T3948 T3943 conj system,chemiluminescence
R3443 T3949 T3948 punct (,system
R3444 T3950 T3951 compound Amersham,Biosciences
R3445 T3951 T3948 appos Biosciences,system
R3446 T3952 T3951 punct ",",Biosciences
R3447 T3953 T3951 conj Bucks,Biosciences
R3448 T3954 T3953 punct ",",Bucks
R3449 T3955 T3953 conj UK,Bucks
R3450 T3956 T3953 punct ),Bucks
R3451 T3957 T3940 cc and,incubated
R3452 T3958 T3940 conj exposed,incubated
R3453 T3959 T3958 prep to,exposed
R3454 T3960 T3961 amod radiographic,film
R3455 T3961 T3959 pobj film,to
R3456 T3962 T3964 punct (,Hyperfilm
R3457 T3963 T3964 compound Fuji,Hyperfilm
R3458 T3964 T3961 appos Hyperfilm,film
R3459 T3965 T3964 punct ",",Hyperfilm
R3460 T3966 T3964 conj Tokyo,Hyperfilm
R3461 T3967 T3966 punct ",",Tokyo
R3462 T3968 T3966 conj Japan,Tokyo
R3463 T3969 T3964 punct ),Hyperfilm
R3464 T3970 T3940 punct .,incubated
R3465 T3971 T3973 nsubjpass ImageJ,used
R3466 T3972 T3973 auxpass was,used
R3467 T3973 T3973 ROOT used,used
R3468 T3974 T3975 aux to,analyze
R3469 T3975 T3973 xcomp analyze,used
R3470 T3976 T3977 det the,intensity
R3471 T3977 T3975 dobj intensity,analyze
R3472 T3978 T3977 prep of,intensity
R3473 T3979 T3980 det the,blots
R3474 T3980 T3978 pobj blots,of
R3475 T3981 T3973 punct .,used
R3476 T3982 T3983 det The,level
R3477 T3983 T3987 nsubjpass level,used
R3478 T3984 T3983 prep of,level
R3479 T3985 T3984 pobj β-actin,of
R3480 T3986 T3987 auxpass was,used
R3481 T3987 T3987 ROOT used,used
R3482 T3988 T3987 prep as,used
R3483 T3989 T3990 amod internal,standard
R3484 T3990 T3988 pobj standard,as
R3485 T3991 T3987 punct .,used
R3249 T3755 T3715 prep in,%
R3250 T3756 T3755 pobj PBS,in
R3251 T3757 T3758 quantmod pH,7.4
R3252 T3758 T3715 dep 7.4,%
R3253 T3759 T3758 punct ),7.4
R3254 T3760 T3715 cc and,%
R3255 T3761 T3715 conj centrifuged,%
R3256 T3762 T3761 prep at,centrifuged
R3257 T3763 T3764 nummod "12,000",g
R3258 T3764 T3762 pobj g,at
R3259 T3765 T3761 prep for,centrifuged
R3260 T3766 T3767 nummod 15,min
R3261 T3767 T3765 pobj min,for
R3262 T3768 T3767 prep at,min
R3263 T3769 T3770 nummod 4,°
R3264 T3770 T3773 nummod °,concentrations
R3265 T3771 T3773 nmod C.,concentrations
R3266 T3772 T3773 compound Protein,concentrations
R3267 T3773 T3768 pobj concentrations,at
R3268 T3774 T3775 auxpass were,estimated
R3269 T3775 T3702 conj estimated,obtain
R3270 T3776 T3775 agent by,estimated
R3271 T3777 T3781 compound Coomassie,Reagent
R3272 T3778 T3779 compound Plus,Protein
R3273 T3779 T3781 compound Protein,Reagent
R3274 T3780 T3781 compound Assay,Reagent
R3275 T3781 T3776 pobj Reagent,by
R3276 T3782 T3783 punct (,Pierce
R3277 T3783 T3781 appos Pierce,Reagent
R3278 T3784 T3783 punct ",",Pierce
R3279 T3785 T3783 prep IL,Pierce
R3280 T3786 T3785 punct ",",IL
R3281 T3787 T3785 npadvmod USA,IL
R3282 T3788 T3783 punct ),Pierce
R3283 T3789 T3775 punct .,estimated
R3284 T3790 T3791 nummod Fifty,micrograms
R3285 T3791 T3798 nsubj micrograms,were
R3286 T3792 T3791 prep of,micrograms
R3287 T3793 T3797 det the,extracts
R3288 T3794 T3797 amod resulting,extracts
R3289 T3795 T3797 amod cytosolic,extracts
R3290 T3796 T3797 compound protein,extracts
R3291 T3797 T3792 pobj extracts,of
R3292 T3798 T3798 ROOT were,were
R3293 T3799 T3798 acomp heat-denatured,were
R3294 T3800 T3799 prep in,heat-denatured
R3295 T3801 T3804 compound Laemmli,buffer
R3296 T3802 T3804 compound sample,buffer
R3334 T3840 T3829 prep at,blocked
R3335 T3841 T3842 nummod 4,°
R3336 T3842 T3843 nummod °,C
R3337 T3843 T3840 pobj C,at
R3338 T3844 T3829 punct ",",blocked
R3339 T3845 T3829 cc and,blocked
R3340 T3846 T3848 det the,antibodies
R3341 T3847 T3848 amod following,antibodies
R3342 T3848 T3850 nsubjpass antibodies,used
R3343 T3849 T3850 auxpass were,used
R3344 T3850 T3829 conj used,blocked
R3345 T3851 T3852 punct :,anti-β-actin
R3346 T3852 T3850 prep anti-β-actin,used
R3347 T3853 T3854 punct (,sc-130657
R3348 T3854 T3852 dep sc-130657,anti-β-actin
R3349 T3855 T3854 punct ",",sc-130657
R3350 T3856 T3858 compound Santa,Biotechnology
R3351 T3857 T3858 compound Cruz,Biotechnology
R3352 T3858 T3854 conj Biotechnology,sc-130657
R3353 T3859 T3858 punct ",",Biotechnology
R3354 T3860 T3858 conj CA,Biotechnology
R3411 T3917 T3914 prep in,washed
R3412 T3918 T3920 det the,buffer
R3413 T3919 T3920 amod washing,buffer
R3414 T3920 T3917 pobj buffer,in
R3415 T3921 T3914 cc and,washed
R3416 T3922 T3914 conj covered,washed
R3417 T3923 T3922 prep with,covered
R3418 T3924 T3928 det the,antibody
R3419 T3925 T3928 amod horseradish,antibody
R3420 T3926 T3928 amod peroxidase-linked,antibody
R3421 T3927 T3928 amod secondary,antibody
R3422 T3928 T3923 pobj antibody,with
R3423 T3929 T3922 prep at,covered
R3424 T3930 T3931 det a,1
R3425 T3931 T3929 pobj 1,at
R3426 T3932 T3934 punct :,dilution
R3427 T3933 T3934 nummod 2000,dilution
R3428 T3934 T3940 nsubjpass dilution,incubated
R3429 T3935 T3934 prep for,dilution
R3430 T3936 T3938 nummod 1,Blots
R3431 T3937 T3938 compound h.,Blots
R3432 T3938 T3935 pobj Blots,for

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T3693 833-863 Protein denotes anti-matrix metallopeptidase 9
T3692 768-773 Protein denotes actin
T3696 1450-1457 Protein denotes β-actin
T3695 1126-1148 Protein denotes horseradish peroxidase
T3694 865-869 Protein denotes MMP9

testone

Id Subject Object Predicate Lexical cue
T3370 865-869 Protein denotes MMP9

test3

Id Subject Object Predicate Lexical cue
T3372 865-869 Protein denotes MMP9
T3371 865-869 Protein denotes MMP9