PMC:3317373 / 7259-7858 JSONTXT

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{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3317373","sourcedb":"PMC","sourceid":"3317373","source_url":"http://www.ncbi.nlm.nih.gov/pmc/3317373","text":"2.5. RT-PCR\nTotal RNA was isolated with Trizol (Invitrogen, CA, USA), and single-stranded cDNA was synthesized from 2 μg of total RNA with BU-Script RT-Kit (Biunique, Jiangsu, China) according to the manufacturer's protocol. The cDNA was stored in −20°C. Reverse transcription was conducted with GoTaq Green Master Mix (Promega, WI, USA) according to the manufacturer's protocol. Table 1 shows the primers and PCR parameters. PCR products were detected by agarose gel electrophoresis. The intensity of the bands was analyzed by ImageJ program. 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