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PMC:3317373 / 5456-7257 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T2662 1794-1800 NN denotes ImageJ
T2661 1785-1793 NN denotes software
T2660 1782-1784 IN denotes by
T2659 1771-1781 VB denotes quantified
T2658 1766-1770 VB denotes were
T2657 1757-1765 NN denotes activity
T2656 1749-1756 NN denotes binding
T2655 1745-1748 NN denotes DNA
T2654 1739-1744 NN denotes NF-κB
T2653 1736-1738 IN denotes of
T2652 1729-1735 NN denotes Levels
T2651 1726-1727 -RRB- denotes )
T2650 1721-1726 NNP denotes Japan
T2649 1719-1720 -COMMA- denotes ,
T2648 1714-1719 NNP denotes Tokyo
T2647 1712-1713 -COMMA- denotes ,
T2646 1703-1712 NNP denotes Hyperfilm
T2645 1698-1702 NNP denotes Fuji
T2644 1697-1698 -LRB- denotes (
T2643 1692-1696 NN denotes film
T2642 1686-1691 NN denotes X-ray
T2641 1683-1685 TO denotes to
T2640 1675-1682 VB denotes exposed
T2639 1671-1674 CC denotes and
T2638 1665-1670 VB denotes dried
T2637 1661-1664 VB denotes was
T2636 1657-1660 NN denotes gel
T2635 1653-1656 DT denotes the
T2634 1651-1652 -COMMA- denotes ,
T2633 1636-1651 NN denotes electrophoresis
T2632 1630-1635 IN denotes After
T2631 1625-1628 NN denotes 4°C
T2630 1622-1624 IN denotes at
T2629 1617-1621 NN denotes hour
T2628 1615-1616 CD denotes 1
T2627 1611-1614 IN denotes for
T2626 1609-1610 NN denotes V
T2625 1605-1608 CD denotes 390
T2624 1602-1604 IN denotes at
T2623 1600-1601 -RRB- denotes )
T2622 1584-1600 NN denotes tris-borate-EDTA
T2621 1583-1584 -LRB- denotes (
T2620 1576-1582 NN denotes buffer
T2619 1572-1575 NN denotes TBE
T2618 1570-1571 SYM denotes ×
T2617 1566-1569 CD denotes 0.5
T2616 1563-1565 IN denotes in
T2615 1559-1562 NN denotes gel
T2614 1544-1558 NN denotes polyacrylamide
T2613 1530-1543 JJ denotes nondenaturing
T2612 1528-1529 NN denotes %
T2611 1527-1528 CD denotes 4
T2610 1524-1526 IN denotes on
T2609 1508-1523 NN denotes electrophoresis
T2608 1505-1507 IN denotes by
T2607 1495-1504 VB denotes separated
T2606 1490-1494 VB denotes were
T2605 1480-1489 NN denotes complexes
T2604 1468-1479 JJ denotes DNA-protein
T2603 1464-1467 DT denotes The
T2602 1459-1462 NN denotes ice
T2601 1456-1458 IN denotes on
T2600 1448-1455 NN denotes minutes
T2599 1445-1447 CD denotes 20
T2598 1441-1444 IN denotes for
T2597 1431-1440 VB denotes continued
T2596 1427-1430 VB denotes was
T2595 1416-1426 NN denotes incubation
T2594 1412-1415 DT denotes the
T2593 1410-1411 -COMMA- denotes ,
T2592 1405-1410 NN denotes probe
T2591 1389-1404 NN denotes oligonucleotide
T2590 1378-1388 JJ denotes 32P-labled
T2589 1375-1377 NN denotes μL
T2588 1373-1374 CD denotes 1
T2587 1369-1372 DT denotes the
T2586 1366-1368 IN denotes of
T2585 1357-1365 NN denotes addition
T2584 1351-1356 IN denotes After
T2583 1346-1349 NN denotes ice
T2582 1343-1345 IN denotes on
T2581 1335-1342 NN denotes minutes
T2580 1332-1334 CD denotes 20
T2579 1328-1331 IN denotes for
T2578 1322-1327 NN denotes dI-dC
T2577 1317-1321 NN denotes poly
T2576 1314-1316 NN denotes μg
T2575 1312-1313 CD denotes 2
T2574 1308-1311 CC denotes and
T2573 1306-1307 -COMMA- denotes ,
T2572 1303-1306 NN denotes DTT
T2571 1300-1302 NN denotes mM
T2570 1296-1299 CD denotes 0.5
T2569 1294-1295 -COMMA- denotes ,
T2568 1290-1294 NN denotes EDTA
T2567 1287-1289 NN denotes mM
T2566 1283-1286 CD denotes 0.5
T2565 1281-1282 -COMMA- denotes ,
T2564 1273-1281 NN denotes glycerol
T2563 1271-1272 NN denotes %
T2562 1270-1271 CD denotes 4
T2561 1268-1269 -COMMA- denotes ,
T2560 1264-1268 NN denotes NaCl
T2559 1261-1263 NN denotes mM
T2558 1257-1260 CD denotes 0.5
T2557 1255-1256 -COMMA- denotes ,
T2493 928-933 VB denotes using
T2492 918-927 VB denotes performed
T2491 914-917 VB denotes was
T2490 909-913 NN denotes EMSA
T2489 906-907 -RRB- denotes )
T2488 903-906 NNP denotes USA
T2487 901-902 -COMMA- denotes ,
T2486 899-901 NNP denotes IL
T2485 897-898 -COMMA- denotes ,
T2484 889-897 NNP denotes Rockford
T2483 887-888 -COMMA- denotes ,
T2482 875-887 NNP denotes Biochemicals
T2481 868-874 NNP denotes Pierce
T2480 867-868 -LRB- denotes (
T2479 858-866 JJ denotes standard
T2478 854-857 DT denotes the
T2477 851-853 IN denotes as
T2476 843-850 NN denotes albumin
T2475 837-842 NN denotes serum
T2474 830-836 JJ denotes bovine
T2473 825-829 IN denotes with
T2472 821-824 NN denotes kit
T2471 815-820 NN denotes assay
T2470 810-814 NN denotes acid
T2469 796-809 JJ denotes bicinchoninic
T2468 794-795 DT denotes a
T2467 788-793 VB denotes using
T2466 777-787 VB denotes determined
T2465 773-776 VB denotes was
T2464 759-772 NN denotes concentration
T2463 751-758 NN denotes Protein
T2462 746-749 NN denotes use
T2461 740-745 IN denotes until
T2460 734-739 NN denotes –80°C
T2459 731-733 IN denotes at
T2458 724-730 VB denotes stored
T2457 720-723 VB denotes was
T2456 711-719 NN denotes proteins
T2455 703-710 JJ denotes nuclear
T2454 699-702 DT denotes the
T2453 688-698 VB denotes containing
T2452 676-687 NN denotes supernatant
T2451 672-675 DT denotes the
T2450 668-671 CC denotes and
T2449 666-667 -COMMA- denotes ,
T2448 663-666 NN denotes rpm
T2447 657-662 CD denotes 14000
T2446 654-656 IN denotes at
T2445 639-653 NN denotes centrifugation
T2444 636-638 IN denotes by
T2443 628-635 VB denotes removed
T2442 624-627 VB denotes was
T2441 615-623 NN denotes material
T2440 605-614 JJ denotes Insoluble
T2439 595-603 NN denotes fluoride
T2438 574-594 NN denotes phenylmethylsulfonyl
T2437 571-573 NN denotes mM
T2436 567-570 CD denotes 1.0
T2435 563-566 CC denotes and
T2434 561-562 -COMMA- denotes ,
T2433 558-561 NN denotes DTT
T2432 555-557 NN denotes mM
T2431 551-554 CD denotes 1.0
T2430 549-550 -COMMA- denotes ,
T2429 545-549 NN denotes EGTA
T2428 542-544 NN denotes mM
T2427 538-541 CD denotes 1.0
T2426 536-537 -COMMA- denotes ,
T2425 532-536 NN denotes EDTA
T2424 529-531 NN denotes mM
T2423 525-528 CD denotes 1.0
T2422 523-524 -COMMA- denotes ,
T2421 519-523 NN denotes NaCl
T2420 516-518 NN denotes mM
T2419 512-515 CD denotes 0.4
T2418 510-511 -COMMA- denotes ,
T2417 509-510 -RRB- denotes )
T2416 506-509 CD denotes 7.9
T2415 503-505 NN denotes pH
T2414 502-503 -LRB- denotes (
T2413 496-501 NN denotes HEPES
T2412 493-495 NN denotes mM
T2411 490-492 CD denotes 20
T2410 479-489 VB denotes containing
T2409 472-478 NN denotes buffer
T2408 467-471 IN denotes with
T2407 457-466 VB denotes extracted
T2406 452-456 VB denotes were
T2405 443-451 NN denotes proteins
T2404 435-442 JJ denotes nuclear
T2403 431-434 DT denotes The
T2402 415-429 NN denotes centrifugation
T2401 412-414 IN denotes by
T2400 403-411 VB denotes pelleted
T2399 398-402 VB denotes were
T2398 391-397 NN denotes nuclei
T2397 387-390 DT denotes the
T2396 383-386 CC denotes and
T2395 381-382 -COMMA- denotes ,
T2394 374-381 NN denotes briefly
T2393 365-373 VB denotes vortexed
T2392 361-364 CC denotes and
T2391 355-360 VB denotes added
T2390 351-354 VB denotes was
T2389 346-350 NN denotes P-40
T2388 338-345 NN denotes Nonidet
T2387 336-337 NN denotes %
T2386 334-336 CD denotes 10
T2385 329-333 RB denotes Then
T2384 319-327 NN denotes fluoride
T2383 298-318 NN denotes phenylmethylsulfonyl
T2382 295-297 NN denotes mM
T2381 291-294 CD denotes 0.5
T2380 287-290 CC denotes and
T2379 285-286 -COMMA- denotes ,
T2378 282-285 NN denotes DTT
T2377 279-281 NN denotes mM
T2376 275-278 CD denotes 1.0
T2375 273-274 -COMMA- denotes ,
T2374 269-273 NN denotes EGTA
T2373 266-268 NN denotes mM
T2372 262-265 CD denotes 0.1
T2371 260-261 -COMMA- denotes ,
T2370 256-260 NN denotes EDTA
T2369 253-255 NN denotes mM
T2368 249-252 CD denotes 0.1
T2367 247-248 -COMMA- denotes ,
T2366 244-247 NN denotes KCl
T2365 241-243 NN denotes mM
T2364 238-240 CD denotes 10
T2363 236-237 -COMMA- denotes ,
T2362 235-236 -RRB- denotes )
T2361 232-235 CD denotes 7.9
T2360 229-231 NN denotes pH
T2359 228-229 -LRB- denotes (
T2358 222-227 NN denotes HEPES
T2357 219-221 NN denotes mM
T2356 216-218 CD denotes 10
T2355 205-215 VB denotes containing
T2354 198-204 NN denotes buffer
T2353 195-197 IN denotes in
T2352 183-194 VB denotes resuspended
T2351 179-182 VB denotes was
T2350 164-178 NN denotes centrifugation
T2349 161-163 IN denotes by
T2348 152-160 VB denotes obtained
T2347 145-151 NN denotes pellet
T2346 140-144 NN denotes cell
T2345 136-139 DT denotes The
T2344 131-134 NNP denotes PBS
T2343 126-130 JJ denotes cold
T2342 121-125 IN denotes into
T2341 112-120 VB denotes scraping
T2340 109-111 IN denotes by
T2339 99-108 VB denotes harvested
T2338 95-98 CC denotes and
T2337 91-94 NNP denotes PBS
T2336 86-90 IN denotes with
T2335 79-85 VB denotes washed
T2334 74-78 VB denotes were
T2333 63-73 NN denotes astrocytes
T2332 60-62 IN denotes of
T2331 49-59 NN denotes Monolayers
T2330 47-48 -RRB- denotes )
T2329 43-47 NN denotes EMSA
T2328 42-43 -LRB- denotes (
T2327 36-41 NN denotes Assay
T2326 30-35 NN denotes Shift
T2325 21-29 NN denotes Mobility
T2324 5-20 JJ denotes Electrophoretic
T2323 0-3 CD denotes 2.4
T2556 1250-1255 NN denotes MgCl2
T2555 1247-1249 NN denotes mM
T2554 1245-1246 CD denotes 1
T2553 1243-1244 -COMMA- denotes ,
T2552 1242-1243 -RRB- denotes )
T2551 1239-1242 CD denotes 7.5
T2550 1236-1238 NN denotes PH
T2549 1235-1236 -LRB- denotes (
T2548 1226-1234 NN denotes Tris-HCl
T2547 1223-1225 NN denotes mM
T2546 1220-1222 CD denotes 10
T2545 1209-1219 VB denotes containing
T2544 1202-1208 NN denotes buffer
T2543 1194-1201 NN denotes binding
T2542 1191-1193 NN denotes μL
T2541 1188-1190 CD denotes 20
T2540 1185-1187 IN denotes in
T2539 1172-1184 VB denotes preincubated
T2538 1168-1171 VB denotes was
T2537 1166-1167 -RRB- denotes )
T2536 1164-1166 NN denotes μg
T2535 1161-1163 CD denotes 20
T2534 1160-1161 -LRB- denotes (
T2533 1152-1159 NN denotes protein
T2532 1144-1151 JJ denotes Nuclear
T2531 1141-1142 -RRB- denotes )
T2530 1136-1141 NNP denotes China
T2529 1134-1135 -COMMA- denotes ,
T2528 1127-1134 NNP denotes Beijing
T2527 1125-1126 -COMMA- denotes ,
T2526 1117-1125 NNP denotes Biotech.
T2525 1112-1116 NNP denotes Free
T2524 1111-1112 -LRB- denotes (
T2523 1106-1110 NN denotes -ATP
T2522 1105-1106 -RRB- denotes ]
T2521 1100-1105 NN denotes γ-32P
T2520 1099-1100 -LRB- denotes [
T2519 1093-1098 VB denotes using
T2518 1086-1092 NN denotes kinase
T2517 1068-1085 NN denotes T4-polynucleotide
T2516 1065-1067 IN denotes by
T2515 1053-1064 VB denotes end-labeled
T2514 1049-1052 VB denotes was
T2513 1047-1048 -RRB- denotes )
T2512 1046-1047 -DQE- denotes ′
T2511 1019-1046 NN denotes 5′-AGTTGAGGGGACTTTCCCAGGG-3
T2510 1018-1019 -LRB- denotes (
T2509 1012-1017 NN denotes probe
T2508 996-1011 NN denotes oligonucleotide
T2507 986-995 NNP denotes Consensus
T2506 983-984 -RRB- denotes )
T2505 980-983 NNP denotes USA
T2504 978-979 -COMMA- denotes ,
T2503 976-978 NNP denotes WI
T2502 974-975 -COMMA- denotes ,
T2501 967-974 NNP denotes Madison
T2500 965-966 -COMMA- denotes ,
T2499 958-965 NNP denotes Promega
T2498 957-958 -LRB- denotes (
T2497 950-956 NN denotes system
T2496 944-949 NN denotes assay
T2495 938-943 NN denotes shift
T2494 934-937 NN denotes gel
R1968 T2327 T2324 arg1Of Assay,Electrophoretic
R1969 T2327 T2325 arg1Of Assay,Mobility
R1970 T2327 T2326 arg1Of Assay,Shift
R1971 T2327 T2328 arg1Of Assay,(
R1972 T2329 T2328 arg2Of EMSA,(
R1973 T2330 T2328 arg3Of ),(
R1974 T2331 T2332 arg1Of Monolayers,of
R1975 T2331 T2334 arg1Of Monolayers,were
R1976 T2331 T2335 arg2Of Monolayers,washed
R1977 T2331 T2339 arg2Of Monolayers,harvested
R1978 T2333 T2332 arg2Of astrocytes,of
R1979 T2335 T2336 arg1Of washed,with
R1980 T2335 T2338 arg1Of washed,and
R1981 T2337 T2336 arg2Of PBS,with
R1982 T2338 T2334 arg2Of and,were
R1983 T2339 T2338 arg2Of harvested,and
R1984 T2339 T2340 arg1Of harvested,by
R1985 T2341 T2340 arg2Of scraping,by
R1986 T2341 T2342 arg1Of scraping,into
R1987 T2344 T2342 arg2Of PBS,into
R1988 T2344 T2343 arg1Of PBS,cold
R1989 T2347 T2345 arg1Of pellet,The
R1990 T2347 T2346 arg1Of pellet,cell
R1991 T2347 T2348 arg2Of pellet,obtained
R1992 T2347 T2351 arg1Of pellet,was
R1993 T2347 T2352 arg2Of pellet,resuspended
R1994 T2350 T2348 arg1Of centrifugation,obtained
R1995 T2350 T2349 arg2Of centrifugation,by
R1996 T2352 T2351 arg2Of resuspended,was
R1997 T2352 T2353 arg1Of resuspended,in
R1998 T2354 T2355 arg1Of buffer,containing
R1999 T2354 T2367 arg1Of buffer,","
R2000 T2356 T2357 arg1Of 10,mM
R2001 T2358 T2356 arg1Of HEPES,10
R2002 T2358 T2359 arg1Of HEPES,(
R2003 T2358 T2363 arg1Of HEPES,","
R2004 T2360 T2359 arg2Of pH,(
R2005 T2360 T2361 arg1Of pH,7.9
R2006 T2362 T2359 arg3Of ),(
R2007 T2363 T2355 arg2Of ",",containing
R2008 T2364 T2365 arg1Of 10,mM
R2009 T2366 T2363 arg2Of KCl,","
R2010 T2366 T2364 arg1Of KCl,10
R2011 T2367 T2353 arg2Of ",",in
R2012 T2368 T2369 arg1Of 0.1,mM
R2013 T2370 T2368 arg1Of EDTA,0.1
R2014 T2370 T2371 arg1Of EDTA,","
R2015 T2371 T2375 arg1Of ",",","
R2016 T2372 T2373 arg1Of 0.1,mM
R2017 T2374 T2371 arg2Of EGTA,","
R2018 T2374 T2372 arg1Of EGTA,0.1
R2019 T2375 T2380 arg1Of ",",and
R2020 T2376 T2377 arg1Of 1.0,mM
R2021 T2378 T2375 arg2Of DTT,","
R2022 T2378 T2376 arg1Of DTT,1.0
R2023 T2380 T2367 arg2Of and,","
R2024 T2380 T2379 arg1Of and,","
R2025 T2381 T2382 arg1Of 0.5,mM
R2026 T2384 T2380 arg2Of fluoride,and
R2027 T2384 T2381 arg1Of fluoride,0.5
R2028 T2384 T2383 arg1Of fluoride,phenylmethylsulfonyl
R2029 T2386 T2385 arg1Of 10,Then
R2030 T2386 T2387 arg1Of 10,%
R2031 T2389 T2386 arg1Of P-40,10
R2032 T2389 T2388 arg1Of P-40,Nonidet
R2033 T2389 T2390 arg1Of P-40,was
R2034 T2389 T2391 arg2Of P-40,added
R2035 T2389 T2393 arg2Of P-40,vortexed
R2036 T2391 T2392 arg1Of added,and
R2037 T2392 T2390 arg2Of and,was
R2038 T2392 T2396 arg1Of and,and
R2039 T2393 T2392 arg2Of vortexed,and
R2040 T2394 T2393 arg3Of briefly,vortexed
R2041 T2396 T2395 arg1Of and,","
R2042 T2398 T2397 arg1Of nuclei,the
R2043 T2398 T2399 arg1Of nuclei,were
R2044 T2398 T2400 arg2Of nuclei,pelleted
R2045 T2400 T2396 arg2Of pelleted,and
R2046 T2400 T2399 arg2Of pelleted,were
R2047 T2402 T2400 arg1Of centrifugation,pelleted
R2048 T2402 T2401 arg2Of centrifugation,by
R2049 T2405 T2403 arg1Of proteins,The
R2050 T2405 T2404 arg1Of proteins,nuclear
R2051 T2405 T2406 arg1Of proteins,were
R2052 T2405 T2407 arg2Of proteins,extracted
R2053 T2407 T2406 arg2Of extracted,were
R2054 T2407 T2408 arg1Of extracted,with
R2055 T2409 T2410 arg1Of buffer,containing
R2056 T2409 T2422 arg1Of buffer,","
R2057 T2411 T2412 arg1Of 20,mM
R2058 T2413 T2411 arg1Of HEPES,20
R2059 T2413 T2414 arg1Of HEPES,(
R2060 T2413 T2418 arg1Of HEPES,","
R2061 T2415 T2414 arg2Of pH,(
R2062 T2415 T2416 arg1Of pH,7.9
R2063 T2417 T2414 arg3Of ),(
R2064 T2418 T2410 arg2Of ",",containing
R2065 T2419 T2420 arg1Of 0.4,mM
R2066 T2421 T2418 arg2Of NaCl,","
R2067 T2421 T2419 arg1Of NaCl,0.4
R2068 T2422 T2408 arg2Of ",",with
R2069 T2423 T2424 arg1Of 1.0,mM
R2070 T2425 T2423 arg1Of EDTA,1.0
R2071 T2425 T2426 arg1Of EDTA,","
R2072 T2426 T2430 arg1Of ",",","
R2073 T2427 T2428 arg1Of 1.0,mM
R2074 T2429 T2426 arg2Of EGTA,","
R2075 T2429 T2427 arg1Of EGTA,1.0
R2076 T2430 T2435 arg1Of ",",and
R2077 T2431 T2432 arg1Of 1.0,mM
R2078 T2433 T2430 arg2Of DTT,","
R2079 T2433 T2431 arg1Of DTT,1.0
R2080 T2435 T2422 arg2Of and,","
R2081 T2435 T2434 arg1Of and,","
R2082 T2436 T2437 arg1Of 1.0,mM
R2083 T2439 T2435 arg2Of fluoride,and
R2084 T2439 T2436 arg1Of fluoride,1.0
R2085 T2439 T2438 arg1Of fluoride,phenylmethylsulfonyl
R2086 T2441 T2440 arg1Of material,Insoluble
R2087 T2441 T2442 arg1Of material,was
R2088 T2441 T2443 arg2Of material,removed
R2089 T2443 T2442 arg2Of removed,was
R2090 T2443 T2446 arg1Of removed,at
R2091 T2443 T2450 arg1Of removed,and
R2092 T2445 T2443 arg1Of centrifugation,removed
R2093 T2445 T2444 arg2Of centrifugation,by
R2094 T2448 T2446 arg2Of rpm,at
R2095 T2448 T2447 arg1Of rpm,14000
R2096 T2450 T2449 arg1Of and,","
R2097 T2452 T2451 arg1Of supernatant,the
R2098 T2452 T2453 arg1Of supernatant,containing
R2099 T2452 T2457 arg1Of supernatant,was
R2100 T2452 T2458 arg2Of supernatant,stored
R2101 T2456 T2453 arg2Of proteins,containing
R2102 T2456 T2454 arg1Of proteins,the
R2103 T2456 T2455 arg1Of proteins,nuclear
R2104 T2458 T2450 arg2Of stored,and
R2105 T2458 T2457 arg2Of stored,was
R2106 T2458 T2459 arg1Of stored,at
R2107 T2458 T2461 arg1Of stored,until
R2108 T2460 T2459 arg2Of –80°C,at
R2109 T2462 T2461 arg2Of use,until
R2110 T2464 T2463 arg1Of concentration,Protein
R2111 T2464 T2465 arg1Of concentration,was
R2112 T2464 T2466 arg2Of concentration,determined
R2113 T2466 T2465 arg2Of determined,was
R2114 T2467 T2466 arg3Of using,determined
R2115 T2467 T2473 arg1Of using,with
R2116 T2472 T2467 arg2Of kit,using
R2117 T2472 T2468 arg1Of kit,a
R2118 T2472 T2469 arg1Of kit,bicinchoninic
R2119 T2472 T2470 arg1Of kit,acid
R2120 T2472 T2471 arg1Of kit,assay
R2121 T2476 T2473 arg2Of albumin,with
R2122 T2476 T2474 arg1Of albumin,bovine
R2123 T2476 T2475 arg1Of albumin,serum
R2124 T2476 T2477 arg1Of albumin,as
R2125 T2482 T2481 arg1Of Biochemicals,Pierce
R2126 T2482 T2483 arg1Of Biochemicals,","
R2127 T2483 T2485 arg1Of ",",","
R2128 T2484 T2483 arg2Of Rockford,","
R2129 T2485 T2477 arg2Of ",",as
R2130 T2485 T2478 arg1Of ",",the
R2131 T2485 T2479 arg1Of ",",standard
R2132 T2485 T2480 arg2Of ",",(
R2133 T2485 T2487 arg1Of ",",","
R2134 T2485 T2488 arg1Of ",",USA
R2135 T2486 T2485 arg2Of IL,","
R2136 T2489 T2480 arg3Of ),(
R2137 T2490 T2491 arg1Of EMSA,was
R2138 T2490 T2492 arg2Of EMSA,performed
R2139 T2492 T2491 arg2Of performed,was
R2140 T2493 T2492 arg3Of using,performed
R2141 T2497 T2493 arg2Of system,using
R2142 T2497 T2494 arg1Of system,gel
R2143 T2497 T2495 arg1Of system,shift
R2144 T2497 T2496 arg1Of system,assay
R2145 T2497 T2498 arg1Of system,(
R2146 T2499 T2500 arg1Of Promega,","
R2147 T2500 T2502 arg1Of ",",","
R2148 T2501 T2500 arg2Of Madison,","
R2149 T2502 T2498 arg2Of ",",(
R2150 T2502 T2504 arg1Of ",",","
R2151 T2502 T2505 arg1Of ",",USA
R2152 T2503 T2502 arg2Of WI,","
R2153 T2506 T2498 arg3Of ),(
R2154 T2509 T2507 arg1Of probe,Consensus
R2155 T2509 T2508 arg1Of probe,oligonucleotide
R2156 T2509 T2510 arg1Of probe,(
R2157 T2509 T2514 arg1Of probe,was
R2158 T2509 T2515 arg2Of probe,end-labeled
R2159 T2509 T2519 arg1Of probe,using
R2160 T2511 T2510 arg2Of 5′-AGTTGAGGGGACTTTCCCAGGG-3,(
R2161 T2511 T2512 arg1Of 5′-AGTTGAGGGGACTTTCCCAGGG-3,′
R2162 T2513 T2510 arg3Of ),(
R2163 T2515 T2514 arg2Of end-labeled,was
R2164 T2515 T2519 modOf end-labeled,using
R2165 T2518 T2515 arg1Of kinase,end-labeled
R2166 T2518 T2516 arg2Of kinase,by
R2167 T2518 T2517 arg1Of kinase,T4-polynucleotide
R2168 T2521 T2520 arg2Of γ-32P,[
R2169 T2522 T2520 arg3Of ],[
R2170 T2523 T2519 arg2Of -ATP,using
R2171 T2523 T2520 arg1Of -ATP,[
R2172 T2523 T2524 arg1Of -ATP,(
R2173 T2526 T2524 arg2Of Biotech.,(
R2174 T2526 T2525 arg1Of Biotech.,Free
R2175 T2526 T2527 arg1Of Biotech.,","
R2176 T2528 T2527 arg2Of Beijing,","
R2177 T2528 T2529 arg1Of Beijing,","
R2178 T2530 T2529 arg2Of China,","
R2179 T2531 T2524 arg3Of ),(
R2180 T2533 T2532 arg1Of protein,Nuclear
R2181 T2533 T2534 arg1Of protein,(
R2182 T2533 T2538 arg1Of protein,was
R2183 T2533 T2539 arg2Of protein,preincubated
R2184 T2536 T2534 arg2Of μg,(
R2185 T2536 T2535 arg1Of μg,20
R2186 T2537 T2534 arg3Of ),(
R2187 T2539 T2538 arg2Of preincubated,was
R2188 T2539 T2540 arg1Of preincubated,in
R2189 T2544 T2540 arg2Of buffer,in
R2190 T2544 T2541 arg1Of buffer,20
R2191 T2544 T2542 arg1Of buffer,μL
R2192 T2544 T2543 arg1Of buffer,binding
R2193 T2544 T2545 arg1Of buffer,containing
R2194 T2546 T2547 arg1Of 10,mM
R2195 T2548 T2546 arg1Of Tris-HCl,10
R2196 T2548 T2549 arg1Of Tris-HCl,(
R2197 T2548 T2553 arg1Of Tris-HCl,","
R2198 T2550 T2549 arg2Of PH,(
R2199 T2550 T2551 arg1Of PH,7.5
R2200 T2552 T2549 arg3Of ),(
R2201 T2553 T2557 arg1Of ",",","
R2202 T2554 T2555 arg1Of 1,mM
R2203 T2556 T2553 arg2Of MgCl2,","
R2204 T2556 T2554 arg1Of MgCl2,1
R2205 T2557 T2561 arg1Of ",",","
R2206 T2558 T2559 arg1Of 0.5,mM
R2207 T2560 T2557 arg2Of NaCl,","
R2208 T2560 T2558 arg1Of NaCl,0.5
R2209 T2561 T2545 arg2Of ",",containing
R2210 T2562 T2563 arg1Of 4,%
R2211 T2564 T2562 arg1Of glycerol,4
R2212 T2564 T2565 arg1Of glycerol,","
R2213 T2565 T2569 arg1Of ",",","
R2214 T2566 T2567 arg1Of 0.5,mM
R2215 T2568 T2565 arg2Of EDTA,","
R2216 T2568 T2566 arg1Of EDTA,0.5
R2217 T2569 T2574 arg1Of ",",and
R2218 T2570 T2571 arg1Of 0.5,mM
R2219 T2572 T2569 arg2Of DTT,","
R2220 T2572 T2570 arg1Of DTT,0.5
R2221 T2574 T2561 arg2Of and,","
R2222 T2574 T2573 arg1Of and,","
R2223 T2578 T2574 arg2Of dI-dC,and
R2224 T2578 T2575 arg1Of dI-dC,2
R2225 T2578 T2576 arg1Of dI-dC,μg
R2226 T2578 T2577 arg1Of dI-dC,poly
R2227 T2578 T2579 arg1Of dI-dC,for
R2228 T2581 T2579 arg2Of minutes,for
R2229 T2581 T2580 arg1Of minutes,20
R2230 T2581 T2582 arg1Of minutes,on
R2231 T2583 T2582 arg2Of ice,on
R2232 T2585 T2584 arg2Of addition,After
R2233 T2585 T2586 arg1Of addition,of
R2234 T2592 T2586 arg2Of probe,of
R2235 T2592 T2587 arg1Of probe,the
R2236 T2592 T2588 arg1Of probe,1
R2237 T2592 T2589 arg1Of probe,μL
R2238 T2592 T2590 arg1Of probe,32P-labled
R2239 T2592 T2591 arg1Of probe,oligonucleotide
R2240 T2595 T2594 arg1Of incubation,the
R2241 T2595 T2596 arg1Of incubation,was
R2242 T2595 T2597 arg2Of incubation,continued
R2243 T2597 T2584 arg1Of continued,After
R2244 T2597 T2593 arg1Of continued,","
R2245 T2597 T2596 arg2Of continued,was
R2246 T2597 T2598 arg1Of continued,for
R2247 T2600 T2598 arg2Of minutes,for
R2248 T2600 T2599 arg1Of minutes,20
R2249 T2600 T2601 arg1Of minutes,on
R2250 T2602 T2601 arg2Of ice,on
R2251 T2605 T2603 arg1Of complexes,The
R2252 T2605 T2604 arg1Of complexes,DNA-protein
R2253 T2605 T2606 arg1Of complexes,were
R2254 T2605 T2607 arg2Of complexes,separated
R2255 T2607 T2606 arg2Of separated,were
R2256 T2607 T2610 arg1Of separated,on
R2257 T2607 T2616 arg1Of separated,in
R2258 T2609 T2607 arg1Of electrophoresis,separated
R2259 T2609 T2608 arg2Of electrophoresis,by
R2260 T2611 T2612 arg1Of 4,%
R2261 T2615 T2610 arg2Of gel,on
R2262 T2615 T2611 arg1Of gel,4
R2263 T2615 T2613 arg1Of gel,nondenaturing
R2264 T2615 T2614 arg1Of gel,polyacrylamide
R2265 T2620 T2616 arg2Of buffer,in
R2266 T2620 T2617 arg1Of buffer,0.5
R2267 T2620 T2618 arg1Of buffer,×
R2268 T2620 T2619 arg1Of buffer,TBE
R2269 T2620 T2621 arg1Of buffer,(
R2270 T2620 T2624 arg1Of buffer,at
R2271 T2622 T2621 arg2Of tris-borate-EDTA,(
R2272 T2623 T2621 arg3Of ),(
R2273 T2626 T2624 arg2Of V,at
R2274 T2626 T2625 arg1Of V,390
R2275 T2626 T2627 arg1Of V,for
R2276 T2629 T2627 arg2Of hour,for
R2277 T2629 T2628 arg1Of hour,1
R2278 T2629 T2630 arg1Of hour,at
R2279 T2631 T2630 arg2Of 4°C,at
R2280 T2633 T2632 arg2Of electrophoresis,After
R2281 T2636 T2635 arg1Of gel,the
R2282 T2636 T2637 arg1Of gel,was
R2283 T2636 T2638 arg2Of gel,dried
R2284 T2636 T2640 arg2Of gel,exposed
R2285 T2638 T2639 arg1Of dried,and
R2286 T2639 T2632 arg1Of and,After
R2287 T2639 T2634 arg1Of and,","
R2288 T2639 T2637 arg2Of and,was
R2289 T2640 T2639 arg2Of exposed,and
R2290 T2640 T2641 arg1Of exposed,to
R2291 T2643 T2641 arg2Of film,to
R2292 T2643 T2642 arg1Of film,X-ray
R2293 T2643 T2644 arg1Of film,(
R2294 T2646 T2644 arg2Of Hyperfilm,(
R2295 T2646 T2645 arg1Of Hyperfilm,Fuji
R2296 T2646 T2647 arg1Of Hyperfilm,","
R2297 T2648 T2647 arg2Of Tokyo,","
R2298 T2648 T2649 arg1Of Tokyo,","
R2299 T2650 T2649 arg2Of Japan,","
R2300 T2651 T2644 arg3Of ),(
R2301 T2652 T2653 arg1Of Levels,of
R2302 T2652 T2658 arg1Of Levels,were
R2303 T2652 T2659 arg2Of Levels,quantified
R2304 T2657 T2653 arg2Of activity,of
R2305 T2657 T2654 arg1Of activity,NF-κB
R2306 T2657 T2655 arg1Of activity,DNA
R2307 T2657 T2656 arg1Of activity,binding
R2308 T2659 T2658 arg2Of quantified,were
R2309 T2662 T2659 arg1Of ImageJ,quantified
R2310 T2662 T2660 arg2Of ImageJ,by
R2311 T2662 T2661 arg1Of ImageJ,software

UBERON-AE

Id Subject Object Predicate Lexical cue
T2308 837-842 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

GO-MF

Id Subject Object Predicate Lexical cue
T2665 1745-1756 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA binding
T2664 1749-1756 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T2663 1194-1201 http://purl.obolibrary.org/obo/GO_0005488 denotes binding

GO-CC

Id Subject Object Predicate Lexical cue
T2670 1472-1489 http://purl.obolibrary.org/obo/GO_0043234 denotes protein complexes
T2669 1468-1489 http://purl.obolibrary.org/obo/GO_0097522 denotes DNA-protein complexes
T2668 1468-1489 http://purl.obolibrary.org/obo/GO_0032993 denotes DNA-protein complexes
T2667 1468-1489 http://purl.obolibrary.org/obo/GO_0001114 denotes DNA-protein complexes
T2666 140-144 http://purl.obolibrary.org/obo/GO_0005623 denotes cell

sentences

Id Subject Object Predicate Lexical cue
T2322 1729-1801 Sentence denotes Levels of NF-κB DNA binding activity were quantified by software ImageJ.
T2321 1464-1728 Sentence denotes The DNA-protein complexes were separated by electrophoresis on 4% nondenaturing polyacrylamide gel in 0.5 × TBE buffer (tris-borate-EDTA) at 390 V for 1 hour at 4°C. After electrophoresis, the gel was dried and exposed to X-ray film (Fuji Hyperfilm, Tokyo, Japan).
T2320 1351-1463 Sentence denotes After addition of the 1 μL 32P-labled oligonucleotide probe, the incubation was continued for 20 minutes on ice.
T2319 1144-1350 Sentence denotes Nuclear protein (20 μg) was preincubated in 20 μL binding buffer containing 10 mM Tris-HCl (PH 7.5), 1 mM MgCl2, 0.5 mM NaCl, 4% glycerol, 0.5 mM EDTA, 0.5 mM DTT, and 2 μg poly dI-dC for 20 minutes on ice.
T2318 986-1143 Sentence denotes Consensus oligonucleotide probe (5′-AGTTGAGGGGACTTTCCCAGGG-3′) was end-labeled by T4-polynucleotide kinase using [γ-32P]-ATP (Free Biotech., Beijing, China).
T2317 909-985 Sentence denotes EMSA was performed using gel shift assay system (Promega, Madison, WI, USA).
T2316 751-908 Sentence denotes Protein concentration was determined using a bicinchoninic acid assay kit with bovine serum albumin as the standard (Pierce Biochemicals, Rockford, IL, USA).
T2315 605-750 Sentence denotes Insoluble material was removed by centrifugation at 14000 rpm, and the supernatant containing the nuclear proteins was stored at –80°C until use.
T2314 431-604 Sentence denotes The nuclear proteins were extracted with buffer containing 20 mM HEPES (pH 7.9), 0.4 mM NaCl, 1.0 mM EDTA, 1.0 mM EGTA, 1.0 mM DTT, and 1.0 mM phenylmethylsulfonyl fluoride.
T2313 329-430 Sentence denotes Then 10% Nonidet P-40 was added and vortexed briefly, and the nuclei were pelleted by centrifugation.
T2312 136-328 Sentence denotes The cell pellet obtained by centrifugation was resuspended in buffer containing 10 mM HEPES (pH 7.9), 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 1.0 mM DTT, and 0.5 mM phenylmethylsulfonyl fluoride.
T2311 49-135 Sentence denotes Monolayers of astrocytes were washed with PBS and harvested by scraping into cold PBS.
T2310 5-48 Sentence denotes Electrophoretic Mobility Shift Assay (EMSA)
T2309 0-4 Sentence denotes 2.4.
T59 0-4 Sentence denotes 2.4.
T60 5-48 Sentence denotes Electrophoretic Mobility Shift Assay (EMSA)
T61 49-135 Sentence denotes Monolayers of astrocytes were washed with PBS and harvested by scraping into cold PBS.
T62 136-328 Sentence denotes The cell pellet obtained by centrifugation was resuspended in buffer containing 10 mM HEPES (pH 7.9), 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 1.0 mM DTT, and 0.5 mM phenylmethylsulfonyl fluoride.
T63 329-430 Sentence denotes Then 10% Nonidet P-40 was added and vortexed briefly, and the nuclei were pelleted by centrifugation.
T64 431-604 Sentence denotes The nuclear proteins were extracted with buffer containing 20 mM HEPES (pH 7.9), 0.4 mM NaCl, 1.0 mM EDTA, 1.0 mM EGTA, 1.0 mM DTT, and 1.0 mM phenylmethylsulfonyl fluoride.
T65 605-750 Sentence denotes Insoluble material was removed by centrifugation at 14000 rpm, and the supernatant containing the nuclear proteins was stored at –80°C until use.
T66 751-908 Sentence denotes Protein concentration was determined using a bicinchoninic acid assay kit with bovine serum albumin as the standard (Pierce Biochemicals, Rockford, IL, USA).
T67 909-985 Sentence denotes EMSA was performed using gel shift assay system (Promega, Madison, WI, USA).
T68 986-1143 Sentence denotes Consensus oligonucleotide probe (5′-AGTTGAGGGGACTTTCCCAGGG-3′) was end-labeled by T4-polynucleotide kinase using [γ-32P]-ATP (Free Biotech., Beijing, China).
T69 1144-1350 Sentence denotes Nuclear protein (20 μg) was preincubated in 20 μL binding buffer containing 10 mM Tris-HCl (PH 7.5), 1 mM MgCl2, 0.5 mM NaCl, 4% glycerol, 0.5 mM EDTA, 0.5 mM DTT, and 2 μg poly dI-dC for 20 minutes on ice.
T70 1351-1463 Sentence denotes After addition of the 1 μL 32P-labled oligonucleotide probe, the incubation was continued for 20 minutes on ice.
T71 1464-1629 Sentence denotes The DNA-protein complexes were separated by electrophoresis on 4% nondenaturing polyacrylamide gel in 0.5 × TBE buffer (tris-borate-EDTA) at 390 V for 1 hour at 4°C.
T72 1630-1728 Sentence denotes After electrophoresis, the gel was dried and exposed to X-ray film (Fuji Hyperfilm, Tokyo, Japan).
T73 1729-1801 Sentence denotes Levels of NF-κB DNA binding activity were quantified by software ImageJ.

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T3095 431-501 Negative_regulation denotes The nuclear proteins were extracted with buffer containing 20 mM HEPES
T3094 275-286 Entity denotes 1.0 mM DTT,
T3093 216-227 Entity denotes 10 mM HEPES
T3092 796-814 Entity denotes bicinchoninic acid
T3091 256-261 Entity denotes EDTA,
T3090 889-907 Entity denotes Rockford, IL, USA)
T3089 1220-1234 Entity denotes 10 mM Tris-HCl
T3088 1317-1321 Entity denotes poly
T3087 1296-1306 Entity denotes 0.5 mM DTT
T3086 751-776 Entity denotes Protein concentration was
T3085 1464-1489 Entity denotes The DNA-protein complexes
T3084 1257-1282 Entity denotes 0.5 mM NaCl, 4% glycerol,
T3083 244-248 Entity denotes KCl,
T3082 830-850 Protein denotes bovine serum albumin
T3081 387-397 Entity denotes the nuclei
T3080 229-231 Protein denotes pH
T3079 1527-1562 Entity denotes 4% nondenaturing polyacrylamide gel
T3078 1283-1307 Entity denotes 0.5 mM EDTA, 0.5 mM DTT,
T3077 1653-1660 Entity denotes the gel
T3076 291-327 Entity denotes 0.5 mM phenylmethylsulfonyl fluoride
T3075 538-562 Entity denotes 1.0 mM EGTA, 1.0 mM DTT,
T3074 1068-1092 Entity denotes T4-polynucleotide kinase
T3062 699-719 Protein denotes the nuclear proteins
T3061 868-887 Entity denotes Pierce Biochemicals
T3060 365-373 Entity denotes vortexed
T3059 431-451 Protein denotes The nuclear proteins
T3058 1071-1092 Protein denotes polynucleotide kinase
T3057 794-824 Entity denotes a bicinchoninic acid assay kit
T3056 1068-1092 Entity denotes T4-polynucleotide kinase
T3055 1106-1126 Entity denotes -ATP (Free Biotech.,
T3054 334-350 Entity denotes 10% Nonidet P-40
T3053 1332-1349 Entity denotes 20 minutes on ice
T3052 1739-1744 Protein denotes NF-κB
T3051 1584-1588 Entity denotes tris
T3050 934-937 Entity denotes gel
T3049 1544-1558 Entity denotes polyacrylamide
T3048 1584-1600 Entity denotes tris-borate-EDTA
T3047 1589-1595 Entity denotes borate
T3046 1220-1234 Entity denotes 10 mM Tris-HCl
T3045 1445-1462 Entity denotes 20 minutes on ice
T3044 136-182 Entity denotes The cell pellet obtained by centrifugation was
T3043 238-273 Entity denotes 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA
T3042 525-536 Entity denotes 1.0 mM EDTA
T3041 551-561 Entity denotes 1.0 mM DTT
T3040 490-501 Entity denotes 20 mM HEPES
T3039 63-73 Entity denotes astrocytes
T3073 1236-1238 Protein denotes PH
T3072 672-719 Entity denotes the supernatant containing the nuclear proteins
T3071 1270-1281 Entity denotes 4% glycerol
T3070 567-603 Entity denotes 1.0 mM phenylmethylsulfonyl fluoride
T3069 1572-1601 Entity denotes TBE buffer (tris-borate-EDTA)
T3068 503-505 Protein denotes pH
T3067 1144-1159 Protein denotes Nuclear protein
T3066 1584-1600 Entity denotes tris-borate-EDTA
T3065 986-1048 Entity denotes Consensus oligonucleotide probe (5′-AGTTGAGGGGACTTTCCCAGGG-3′)
T3064 1366-1410 Entity denotes of the 1 μL 32P-labled oligonucleotide probe
T3063 512-537 Entity denotes 0.4 mM NaCl, 1.0 mM EDTA,
R2676 T3059 T3095 themeOf The nuclear proteins,The nuclear proteins were extracted with buffer containing 20 mM HEPES

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T3035 1800-1801 . denotes .
T3034 1794-1800 NNP denotes ImageJ
T3033 1785-1793 NN denotes software
T3032 1782-1784 IN denotes by
T3031 1771-1781 VBN denotes quantified
T3030 1766-1770 VBD denotes were
T3029 1757-1765 NN denotes activity
T3028 1749-1756 JJ denotes binding
T3027 1745-1748 NNP denotes DNA
T3026 1739-1744 NNP denotes NF-κB
T3025 1736-1738 IN denotes of
T3024 1729-1735 NNS denotes Levels
T3023 1727-1728 . denotes .
T3022 1726-1727 -RRB- denotes )
T3021 1721-1726 NNP denotes Japan
T3020 1719-1720 , denotes ,
T3019 1714-1719 NNP denotes Tokyo
T3018 1712-1713 , denotes ,
T3017 1703-1712 NNP denotes Hyperfilm
T3016 1698-1702 NNP denotes Fuji
T3015 1697-1698 -LRB- denotes (
T3014 1692-1696 NN denotes film
T3013 1686-1691 NN denotes X-ray
T3012 1683-1685 TO denotes to
T3011 1675-1682 VBN denotes exposed
T3010 1671-1674 CC denotes and
T3009 1665-1670 VBN denotes dried
T3008 1661-1664 VBD denotes was
T3007 1657-1660 NN denotes gel
T3006 1653-1656 DT denotes the
T3005 1651-1652 , denotes ,
T3004 1636-1651 NN denotes electrophoresis
T3003 1630-1635 IN denotes After
T3002 1628-1629 . denotes .
T3001 1627-1628 NNP denotes C
T3000 1626-1627 CD denotes °
T2999 1625-1626 CD denotes 4
T2998 1622-1624 IN denotes at
T2997 1617-1621 NN denotes hour
T2996 1615-1616 CD denotes 1
T2995 1611-1614 IN denotes for
T2994 1609-1610 NN denotes V
T2993 1605-1608 CD denotes 390
T2992 1602-1604 IN denotes at
T2991 1600-1601 -RRB- denotes )
T2990 1584-1600 NNP denotes tris-borate-EDTA
T2989 1583-1584 -LRB- denotes (
T2988 1576-1582 NN denotes buffer
T2987 1572-1575 NNP denotes TBE
T2986 1570-1571 CD denotes ×
T2985 1566-1569 CD denotes 0.5
T2984 1563-1565 IN denotes in
T2983 1559-1562 NN denotes gel
T2982 1544-1558 NN denotes polyacrylamide
T2981 1530-1543 JJ denotes nondenaturing
T2980 1528-1529 NN denotes %
T2979 1527-1528 CD denotes 4
T2978 1524-1526 IN denotes on
T2977 1508-1523 NN denotes electrophoresis
T2976 1505-1507 IN denotes by
T2975 1495-1504 VBN denotes separated
T2974 1490-1494 VBD denotes were
T2973 1480-1489 NNS denotes complexes
T2972 1468-1479 JJ denotes DNA-protein
T2971 1464-1467 DT denotes The
T2970 1462-1463 . denotes .
T2969 1459-1462 NN denotes ice
T2968 1456-1458 IN denotes on
T2967 1448-1455 NNS denotes minutes
T2966 1445-1447 CD denotes 20
T2965 1441-1444 IN denotes for
T2964 1431-1440 VBN denotes continued
T2963 1427-1430 VBD denotes was
T2962 1416-1426 NN denotes incubation
T2961 1412-1415 DT denotes the
T2960 1410-1411 , denotes ,
T2959 1405-1410 NN denotes probe
T2958 1389-1404 NN denotes oligonucleotide
T2957 1378-1388 JJ denotes 32P-labled
T2956 1375-1377 NNP denotes μL
T2955 1373-1374 CD denotes 1
T2954 1369-1372 DT denotes the
T2953 1366-1368 IN denotes of
T2952 1357-1365 NN denotes addition
T2951 1351-1356 IN denotes After
T2950 1349-1350 . denotes .
T2949 1346-1349 NN denotes ice
T2948 1343-1345 IN denotes on
T2947 1335-1342 NNS denotes minutes
T2946 1332-1334 CD denotes 20
T2945 1328-1331 IN denotes for
T2944 1322-1327 NN denotes dI-dC
T2943 1317-1321 NN denotes poly
T2942 1314-1316 NN denotes μg
T2941 1312-1313 CD denotes 2
T2940 1308-1311 CC denotes and
T2939 1306-1307 , denotes ,
T2938 1303-1306 NNP denotes DTT
T2937 1300-1302 NNP denotes mM
T2936 1296-1299 CD denotes 0.5
T2935 1294-1295 , denotes ,
T2934 1290-1294 NNP denotes EDTA
T2933 1287-1289 NNP denotes mM
T2932 1283-1286 CD denotes 0.5
T2931 1281-1282 , denotes ,
T2930 1273-1281 NN denotes glycerol
T2929 1271-1272 NN denotes %
T2928 1270-1271 CD denotes 4
T2927 1268-1269 , denotes ,
T2926 1264-1268 NNP denotes NaCl
T2925 1261-1263 NNP denotes mM
T2924 1257-1260 CD denotes 0.5
T2923 1255-1256 , denotes ,
T2922 1250-1255 NNP denotes MgCl2
T2921 1247-1249 NNP denotes mM
T2920 1245-1246 CD denotes 1
T2919 1243-1244 , denotes ,
T2918 1242-1243 -RRB- denotes )
T2917 1239-1242 CD denotes 7.5
T2916 1236-1238 NNP denotes PH
T2915 1235-1236 -LRB- denotes (
T2914 1226-1234 NNP denotes Tris-HCl
T2913 1223-1225 NNP denotes mM
T2912 1220-1222 CD denotes 10
T2911 1209-1219 VBG denotes containing
T2910 1202-1208 NN denotes buffer
T2909 1194-1201 JJ denotes binding
T2908 1191-1193 NN denotes μL
T2907 1188-1190 CD denotes 20
T2906 1185-1187 IN denotes in
T2905 1172-1184 VBN denotes preincubated
T2904 1168-1171 VBD denotes was
T2903 1166-1167 -RRB- denotes )
T2902 1164-1166 NN denotes μg
T2901 1161-1163 CD denotes 20
T2900 1160-1161 -LRB- denotes (
T2899 1152-1159 NN denotes protein
T2898 1144-1151 NNP denotes Nuclear
T2897 1142-1143 . denotes .
T2896 1141-1142 -RRB- denotes )
T2895 1136-1141 NNP denotes China
T2894 1134-1135 , denotes ,
T2893 1127-1134 NNP denotes Beijing
T2892 1125-1126 , denotes ,
T2891 1117-1125 NNP denotes Biotech.
T2890 1112-1116 NNP denotes Free
T2889 1111-1112 -LRB- denotes (
T2888 1107-1110 NNP denotes ATP
T2887 1106-1107 : denotes -
T2886 1105-1106 NNP denotes ]
T2885 1100-1105 NNP denotes γ-32P
T2884 1099-1100 NNP denotes [
T2883 1093-1098 VBG denotes using
T2882 1086-1092 NN denotes kinase
T2881 1068-1085 NN denotes T4-polynucleotide
T2880 1065-1067 IN denotes by
T2879 1053-1064 VBN denotes end-labeled
T2878 1049-1052 VBD denotes was
T2877 1047-1048 -RRB- denotes )
T2876 1046-1047 NN denotes ′
T2875 1022-1046 NN denotes AGTTGAGGGGACTTTCCCAGGG-3
T2874 1021-1022 : denotes -
T2873 1020-1021 SYM denotes ′
T2872 1019-1020 CD denotes 5
T2871 1018-1019 -LRB- denotes (
T2870 1012-1017 NN denotes probe
T2869 996-1011 NN denotes oligonucleotide
T2868 986-995 NNP denotes Consensus
T2867 984-985 . denotes .
T2866 983-984 -RRB- denotes )
T2865 980-983 NNP denotes USA
T2864 978-979 , denotes ,
T2863 976-978 NNP denotes WI
T2862 974-975 , denotes ,
T2861 967-974 NNP denotes Madison
T2860 965-966 , denotes ,
T2859 958-965 NNP denotes Promega
T2858 957-958 -LRB- denotes (
T2857 950-956 NN denotes system
T2856 944-949 NN denotes assay
T2855 938-943 NN denotes shift
T2854 934-937 NN denotes gel
T2853 928-933 VBG denotes using
T2852 918-927 VBN denotes performed
T2851 914-917 VBD denotes was
T2850 909-913 NNP denotes EMSA
T2849 907-908 . denotes .
T2848 906-907 -RRB- denotes )
T2847 903-906 NNP denotes USA
T2772 509-510 -RRB- denotes )
T2771 506-509 CD denotes 7.9
T2770 503-505 NNP denotes pH
T2769 502-503 -LRB- denotes (
T2768 496-501 NNP denotes HEPES
T2767 493-495 NNP denotes mM
T2766 490-492 CD denotes 20
T2765 479-489 VBG denotes containing
T2764 472-478 NN denotes buffer
T2763 467-471 IN denotes with
T2762 457-466 VBN denotes extracted
T2761 452-456 VBD denotes were
T2760 443-451 NNS denotes proteins
T2759 435-442 JJ denotes nuclear
T2758 431-434 DT denotes The
T2757 429-430 . denotes .
T2756 415-429 NN denotes centrifugation
T2755 412-414 IN denotes by
T2754 403-411 VBN denotes pelleted
T2753 398-402 VBD denotes were
T2752 391-397 NNS denotes nuclei
T2751 387-390 DT denotes the
T2750 383-386 CC denotes and
T2749 381-382 , denotes ,
T2748 374-381 NN denotes briefly
T2747 365-373 VBN denotes vortexed
T2746 361-364 CC denotes and
T2745 355-360 VBN denotes added
T2744 351-354 VBD denotes was
T2743 346-350 NNP denotes P-40
T2742 338-345 NNP denotes Nonidet
T2741 336-337 NN denotes %
T2740 334-336 CD denotes 10
T2739 329-333 RB denotes Then
T2738 327-328 . denotes .
T2737 319-327 NN denotes fluoride
T2736 298-318 NN denotes phenylmethylsulfonyl
T2735 295-297 NNP denotes mM
T2734 291-294 CD denotes 0.5
T2733 287-290 CC denotes and
T2732 285-286 , denotes ,
T2731 282-285 NNP denotes DTT
T2730 279-281 NNP denotes mM
T2729 275-278 CD denotes 1.0
T2728 273-274 , denotes ,
T2727 269-273 NNP denotes EGTA
T2726 266-268 NNP denotes mM
T2725 262-265 CD denotes 0.1
T2724 260-261 , denotes ,
T2723 256-260 NNP denotes EDTA
T2722 253-255 NNP denotes mM
T2721 249-252 CD denotes 0.1
T2720 247-248 , denotes ,
T2719 244-247 NNP denotes KCl
T2718 241-243 NNP denotes mM
T2717 238-240 CD denotes 10
T2716 236-237 , denotes ,
T2715 235-236 -RRB- denotes )
T2714 232-235 CD denotes 7.9
T2713 229-231 NNP denotes pH
T2712 228-229 -LRB- denotes (
T2711 222-227 NNP denotes HEPES
T2710 219-221 NNP denotes mM
T2709 216-218 CD denotes 10
T2708 205-215 VBG denotes containing
T2707 198-204 NN denotes buffer
T2706 195-197 IN denotes in
T2705 183-194 VBN denotes resuspended
T2704 179-182 VBD denotes was
T2703 164-178 NN denotes centrifugation
T2702 161-163 IN denotes by
T2701 152-160 VBN denotes obtained
T2700 145-151 NN denotes pellet
T2699 140-144 NN denotes cell
T2698 136-139 DT denotes The
T2697 134-135 . denotes .
T2696 131-134 NNP denotes PBS
T2695 126-130 JJ denotes cold
T2694 121-125 IN denotes into
T2693 112-120 VBG denotes scraping
T2692 109-111 IN denotes by
T2691 99-108 VBN denotes harvested
T2690 95-98 CC denotes and
T2689 91-94 NNP denotes PBS
T2688 86-90 IN denotes with
T2687 79-85 VBN denotes washed
T2686 74-78 VBD denotes were
T2685 63-73 NNS denotes astrocytes
T2684 60-62 IN denotes of
T2683 49-59 NNPS denotes Monolayers
T2682 47-48 -RRB- denotes )
T2681 43-47 NNP denotes EMSA
T2680 42-43 -LRB- denotes (
T2679 36-41 NNP denotes Assay
T2678 30-35 NNP denotes Shift
T2677 21-29 NNP denotes Mobility
T2676 5-20 NNP denotes Electrophoretic
T2675 3-4 . denotes .
T2674 0-3 CD denotes 2.4
T2846 901-902 , denotes ,
T2845 899-901 NNP denotes IL
T2844 897-898 , denotes ,
T2843 889-897 NNP denotes Rockford
T2842 887-888 , denotes ,
T2841 875-887 NNP denotes Biochemicals
T2840 868-874 NNP denotes Pierce
T2839 867-868 -LRB- denotes (
T2838 858-866 NN denotes standard
T2837 854-857 DT denotes the
T2836 851-853 IN denotes as
T2835 843-850 NN denotes albumin
T2834 837-842 NN denotes serum
T2833 830-836 JJ denotes bovine
T2832 825-829 IN denotes with
T2831 821-824 NN denotes kit
T2830 815-820 NN denotes assay
T2829 810-814 NN denotes acid
T2828 796-809 JJ denotes bicinchoninic
T2827 794-795 DT denotes a
T2826 788-793 VBG denotes using
T2825 777-787 VBN denotes determined
T2824 773-776 VBD denotes was
T2823 759-772 NN denotes concentration
T2822 751-758 NN denotes Protein
T2821 749-750 . denotes .
T2820 746-749 NN denotes use
T2819 740-745 IN denotes until
T2818 738-739 NNP denotes C
T2817 737-738 CD denotes °
T2816 735-737 CD denotes 80
T2815 731-733 IN denotes at
T2814 724-730 VBN denotes stored
T2813 720-723 VBD denotes was
T2812 711-719 NNS denotes proteins
T2811 703-710 JJ denotes nuclear
T2810 699-702 DT denotes the
T2809 688-698 VBG denotes containing
T2808 676-687 JJ denotes supernatant
T2807 672-675 DT denotes the
T2806 668-671 CC denotes and
T2805 666-667 , denotes ,
T2804 663-666 NN denotes rpm
T2803 657-662 CD denotes 14000
T2802 654-656 IN denotes at
T2801 639-653 NN denotes centrifugation
T2800 636-638 IN denotes by
T2799 628-635 VBN denotes removed
T2798 624-627 VBD denotes was
T2797 615-623 NN denotes material
T2796 605-614 NNP denotes Insoluble
T2795 603-604 . denotes .
T2794 595-603 NN denotes fluoride
T2793 574-594 NN denotes phenylmethylsulfonyl
T2792 571-573 NNP denotes mM
T2791 567-570 CD denotes 1.0
T2790 563-566 CC denotes and
T2789 561-562 , denotes ,
T2788 558-561 NNP denotes DTT
T2787 555-557 NNP denotes mM
T2786 551-554 CD denotes 1.0
T2785 549-550 , denotes ,
T2784 545-549 NNP denotes EGTA
T2783 542-544 NNP denotes mM
T2782 538-541 CD denotes 1.0
T2781 536-537 , denotes ,
T2780 532-536 NNP denotes EDTA
T2779 529-531 NNP denotes mM
T2778 525-528 CD denotes 1.0
T2777 523-524 , denotes ,
T2776 519-523 NNP denotes NaCl
T2775 516-518 NNP denotes mM
T2774 512-515 CD denotes 0.4
T2773 510-511 , denotes ,
R2313 T2674 T2674 ROOT 2.4,2.4
R2314 T2675 T2674 punct .,2.4
R2315 T2676 T2679 compound Electrophoretic,Assay
R2316 T2677 T2678 compound Mobility,Shift
R2317 T2678 T2679 compound Shift,Assay
R2318 T2679 T2687 nsubjpass Assay,washed
R2319 T2680 T2681 punct (,EMSA
R2320 T2681 T2679 appos EMSA,Assay
R2321 T2682 T2681 punct ),EMSA
R2322 T2683 T2679 appos Monolayers,Assay
R2323 T2684 T2683 prep of,Monolayers
R2324 T2685 T2684 pobj astrocytes,of
R2325 T2686 T2687 auxpass were,washed
R2326 T2687 T2687 ROOT washed,washed
R2327 T2688 T2687 prep with,washed
R2328 T2689 T2688 pobj PBS,with
R2329 T2690 T2687 cc and,washed
R2330 T2691 T2687 conj harvested,washed
R2331 T2692 T2691 prep by,harvested
R2332 T2693 T2692 pcomp scraping,by
R2333 T2694 T2693 prep into,scraping
R2334 T2695 T2696 amod cold,PBS
R2335 T2696 T2694 pobj PBS,into
R2336 T2697 T2687 punct .,washed
R2337 T2698 T2700 det The,pellet
R2338 T2699 T2700 compound cell,pellet
R2339 T2700 T2704 nsubj pellet,was
R2340 T2701 T2700 acl obtained,pellet
R2341 T2702 T2701 agent by,obtained
R2342 T2703 T2702 pobj centrifugation,by
R2343 T2704 T2705 auxpass was,resuspended
R2344 T2705 T2705 ROOT resuspended,resuspended
R2345 T2706 T2705 prep in,resuspended
R2346 T2707 T2706 pobj buffer,in
R2347 T2708 T2707 acl containing,buffer
R2348 T2709 T2711 nummod 10,HEPES
R2349 T2710 T2711 compound mM,HEPES
R2350 T2711 T2708 dobj HEPES,containing
R2351 T2712 T2713 punct (,pH
R2352 T2713 T2711 appos pH,HEPES
R2353 T2714 T2713 nummod 7.9,pH
R2359 T2720 T2719 punct ",",KCl
R2360 T2721 T2723 compound 0.1,EDTA
R2361 T2722 T2723 compound mM,EDTA
R2362 T2723 T2719 conj EDTA,KCl
R2363 T2724 T2723 punct ",",EDTA
R2364 T2725 T2727 nummod 0.1,EGTA
R2365 T2726 T2727 compound mM,EGTA
R2366 T2727 T2723 conj EGTA,EDTA
R2367 T2728 T2727 punct ",",EGTA
R2368 T2729 T2731 nummod 1.0,DTT
R2369 T2730 T2731 compound mM,DTT
R2370 T2731 T2727 conj DTT,EGTA
R2371 T2732 T2731 punct ",",DTT
R2372 T2733 T2731 cc and,DTT
R2373 T2734 T2737 nummod 0.5,fluoride
R2374 T2735 T2737 nmod mM,fluoride
R2375 T2736 T2737 compound phenylmethylsulfonyl,fluoride
R2376 T2737 T2731 conj fluoride,DTT
R2377 T2738 T2705 punct .,resuspended
R2378 T2739 T2745 advmod Then,added
R2379 T2740 T2741 nummod 10,%
R2380 T2741 T2743 compound %,P-40
R2381 T2742 T2743 compound Nonidet,P-40
R2382 T2743 T2745 nsubjpass P-40,added
R2383 T2744 T2745 auxpass was,added
R2384 T2745 T2745 ROOT added,added
R2385 T2746 T2745 cc and,added
R2386 T2747 T2745 conj vortexed,added
R2387 T2748 T2747 advmod briefly,vortexed
R2388 T2749 T2747 punct ",",vortexed
R2389 T2750 T2747 cc and,vortexed
R2390 T2751 T2752 det the,nuclei
R2391 T2752 T2754 nsubjpass nuclei,pelleted
R2392 T2753 T2754 auxpass were,pelleted
R2393 T2754 T2747 conj pelleted,vortexed
R2394 T2755 T2754 agent by,pelleted
R2395 T2756 T2755 pobj centrifugation,by
R2396 T2757 T2754 punct .,pelleted
R2397 T2758 T2760 det The,proteins
R2398 T2759 T2760 amod nuclear,proteins
R2399 T2760 T2762 nsubjpass proteins,extracted
R2400 T2761 T2762 auxpass were,extracted
R2401 T2762 T2762 ROOT extracted,extracted
R2402 T2763 T2762 prep with,extracted
R2403 T2764 T2763 pobj buffer,with
R2404 T2765 T2764 acl containing,buffer
R2405 T2766 T2768 nummod 20,HEPES
R2406 T2767 T2768 compound mM,HEPES
R2407 T2768 T2765 dobj HEPES,containing
R2408 T2769 T2770 punct (,pH
R2409 T2770 T2768 appos pH,HEPES
R2410 T2771 T2770 nummod 7.9,pH
R2411 T2772 T2770 punct ),pH
R2412 T2773 T2768 punct ",",HEPES
R2413 T2774 T2776 nummod 0.4,NaCl
R2414 T2775 T2776 compound mM,NaCl
R2415 T2776 T2768 appos NaCl,HEPES
R2416 T2777 T2776 punct ",",NaCl
R2417 T2778 T2780 nummod 1.0,EDTA
R2418 T2779 T2780 compound mM,EDTA
R2419 T2780 T2776 conj EDTA,NaCl
R2420 T2781 T2780 punct ",",EDTA
R2421 T2782 T2784 nummod 1.0,EGTA
R2422 T2783 T2784 compound mM,EGTA
R2423 T2784 T2780 conj EGTA,EDTA
R2424 T2785 T2784 punct ",",EGTA
R2425 T2786 T2788 nummod 1.0,DTT
R2452 T2813 T2814 auxpass was,stored
R2453 T2814 T2799 conj stored,removed
R2454 T2815 T2814 prep at,stored
R2455 T2816 T2818 nummod 80,C
R2456 T2817 T2818 nummod °,C
R2457 T2818 T2815 pobj C,at
R2458 T2819 T2814 prep until,stored
R2459 T2820 T2819 pobj use,until
R2460 T2821 T2814 punct .,stored
R2461 T2822 T2823 compound Protein,concentration
R2462 T2823 T2825 nsubjpass concentration,determined
R2463 T2824 T2825 auxpass was,determined
R2464 T2825 T2825 ROOT determined,determined
R2465 T2826 T2825 advcl using,determined
R2466 T2827 T2831 det a,kit
R2467 T2828 T2831 amod bicinchoninic,kit
R2468 T2829 T2830 compound acid,assay
R2469 T2830 T2831 compound assay,kit
R2470 T2831 T2826 dobj kit,using
R2471 T2832 T2831 prep with,kit
R2472 T2833 T2834 amod bovine,serum
R2473 T2834 T2832 pobj serum,with
R2474 T2835 T2831 amod albumin,kit
R2475 T2836 T2826 prep as,using
R2476 T2837 T2838 det the,standard
R2477 T2838 T2836 pobj standard,as
R2478 T2839 T2841 punct (,Biochemicals
R2479 T2840 T2841 compound Pierce,Biochemicals
R2480 T2841 T2826 parataxis Biochemicals,using
R2481 T2842 T2841 punct ",",Biochemicals
R2482 T2843 T2841 npadvmod Rockford,Biochemicals
R2483 T2844 T2843 punct ",",Rockford
R2484 T2845 T2843 conj IL,Rockford
R2485 T2846 T2845 punct ",",IL
R2486 T2847 T2843 npadvmod USA,Rockford
R2487 T2848 T2843 punct ),Rockford
R2488 T2849 T2841 punct .,Biochemicals
R2489 T2850 T2852 nsubjpass EMSA,performed
R2490 T2851 T2852 auxpass was,performed
R2491 T2852 T2852 ROOT performed,performed
R2492 T2853 T2852 advcl using,performed
R2493 T2854 T2855 compound gel,shift
R2494 T2855 T2857 compound shift,system
R2495 T2856 T2857 compound assay,system
R2496 T2857 T2853 dobj system,using
R2497 T2858 T2857 punct (,system
R2498 T2859 T2857 appos Promega,system
R2499 T2860 T2859 punct ",",Promega
R2500 T2861 T2859 conj Madison,Promega
R2501 T2862 T2861 punct ",",Madison
R2502 T2863 T2861 conj WI,Madison
R2503 T2864 T2863 punct ",",WI
R2518 T2879 T2879 ROOT end-labeled,end-labeled
R2519 T2880 T2879 agent by,end-labeled
R2520 T2881 T2882 compound T4-polynucleotide,kinase
R2521 T2882 T2880 pobj kinase,by
R2522 T2883 T2879 advcl using,end-labeled
R2523 T2884 T2888 compound [,ATP
R2524 T2885 T2886 compound γ-32P,]
R2525 T2886 T2888 compound ],ATP
R2526 T2887 T2888 punct -,ATP
R2527 T2888 T2883 dobj ATP,using
R2528 T2889 T2891 punct (,Biotech.
R2529 T2890 T2891 compound Free,Biotech.
R2530 T2891 T2888 appos Biotech.,ATP
R2531 T2892 T2891 punct ",",Biotech.
R2532 T2893 T2891 npadvmod Beijing,Biotech.
R2533 T2894 T2893 punct ",",Beijing
R2534 T2895 T2891 npadvmod China,Biotech.
R2535 T2896 T2891 punct ),Biotech.
R2536 T2897 T2879 punct .,end-labeled
R2537 T2898 T2899 compound Nuclear,protein
R2538 T2899 T2904 nsubj protein,was
R2539 T2900 T2899 punct (,protein
R2540 T2901 T2902 nummod 20,μg
R2541 T2902 T2899 appos μg,protein
R2542 T2903 T2899 punct ),protein
R2543 T2904 T2905 auxpass was,preincubated
R2544 T2905 T2905 ROOT preincubated,preincubated
R2545 T2906 T2905 prep in,preincubated
R2546 T2907 T2908 nummod 20,μL
R2547 T2908 T2910 nmod μL,buffer
R2548 T2909 T2910 amod binding,buffer
R2549 T2910 T2906 pobj buffer,in
R2550 T2911 T2910 acl containing,buffer
R2551 T2912 T2914 nummod 10,Tris-HCl
R2552 T2913 T2914 compound mM,Tris-HCl
R2553 T2914 T2911 dobj Tris-HCl,containing
R2554 T2915 T2916 punct (,PH
R2555 T2916 T2914 appos PH,Tris-HCl
R2556 T2917 T2916 nummod 7.5,PH
R2557 T2918 T2916 punct ),PH
R2558 T2919 T2914 punct ",",Tris-HCl
R2559 T2920 T2922 nummod 1,MgCl2
R2560 T2921 T2922 compound mM,MgCl2
R2561 T2922 T2914 conj MgCl2,Tris-HCl
R2562 T2923 T2922 punct ",",MgCl2
R2563 T2924 T2922 npadvmod 0.5,MgCl2
R2564 T2925 T2926 compound mM,NaCl
R2565 T2926 T2926 ROOT NaCl,NaCl
R2566 T2927 T2926 punct ",",NaCl
R2567 T2928 T2929 nummod 4,%
R2568 T2929 T2930 compound %,glycerol
R2569 T2930 T2926 appos glycerol,NaCl
R2570 T2931 T2930 punct ",",glycerol
R2571 T2932 T2934 nummod 0.5,EDTA
R2572 T2933 T2934 compound mM,EDTA
R2573 T2934 T2926 conj EDTA,NaCl
R2574 T2935 T2934 punct ",",EDTA
R2575 T2936 T2938 nummod 0.5,DTT
R2576 T2937 T2938 compound mM,DTT
R2577 T2938 T2934 conj DTT,EDTA
R2578 T2939 T2938 punct ",",DTT
R2579 T2940 T2938 cc and,DTT
R2580 T2941 T2942 nummod 2,μg
R2581 T2942 T2944 compound μg,dI-dC
R2582 T2943 T2944 compound poly,dI-dC
R2583 T2944 T2934 appos dI-dC,EDTA
R2584 T2945 T2944 prep for,dI-dC
R2585 T2946 T2947 nummod 20,minutes
R2586 T2947 T2945 pobj minutes,for
R2587 T2948 T2947 prep on,minutes
R2588 T2949 T2948 pobj ice,on
R2589 T2950 T2926 punct .,NaCl
R2590 T2951 T2964 prep After,continued
R2591 T2952 T2951 pobj addition,After
R2592 T2953 T2952 prep of,addition
R2593 T2954 T2959 det the,probe
R2594 T2955 T2956 nummod 1,μL
R2611 T2972 T2973 amod DNA-protein,complexes
R2612 T2973 T2975 nsubjpass complexes,separated
R2613 T2974 T2975 auxpass were,separated
R2614 T2975 T2975 ROOT separated,separated
R2615 T2976 T2975 agent by,separated
R2616 T2977 T2976 pobj electrophoresis,by
R2617 T2978 T2975 prep on,separated
R2618 T2979 T2980 nummod 4,%
R2619 T2980 T2983 nmod %,gel
R2620 T2981 T2983 amod nondenaturing,gel
R2621 T2982 T2983 compound polyacrylamide,gel
R2622 T2983 T2978 pobj gel,on
R2623 T2984 T2975 prep in,separated
R2624 T2985 T2988 nummod 0.5,buffer
R2625 T2986 T2988 nummod ×,buffer
R2626 T2987 T2988 compound TBE,buffer
R2627 T2988 T2984 pobj buffer,in
R2628 T2989 T2988 punct (,buffer
R2629 T2990 T2988 appos tris-borate-EDTA,buffer
R2630 T2991 T2988 punct ),buffer
R2631 T2992 T2975 prep at,separated
R2632 T2993 T2994 nummod 390,V
R2633 T2994 T2992 pobj V,at
R2634 T2995 T2975 prep for,separated
R2635 T2996 T2997 nummod 1,hour
R2636 T2997 T2995 pobj hour,for
R2637 T2998 T2975 prep at,separated
R2638 T2999 T3000 nummod 4,°
R2664 T3025 T3024 prep of,Levels
R2665 T3026 T3027 compound NF-κB,DNA
R2666 T3027 T3025 pobj DNA,of
R2667 T3028 T3029 amod binding,activity
R2668 T3029 T3025 pobj activity,of
R2669 T3030 T3031 auxpass were,quantified
R2670 T3031 T3031 ROOT quantified,quantified
R2671 T3032 T3031 agent by,quantified
R2672 T3033 T3034 compound software,ImageJ
R2673 T3034 T3032 pobj ImageJ,by
R2674 T3035 T3031 punct .,quantified
R2354 T2715 T2713 punct ),pH
R2355 T2716 T2711 punct ",",HEPES
R2356 T2717 T2719 nummod 10,KCl
R2357 T2718 T2719 compound mM,KCl
R2358 T2719 T2711 conj KCl,HEPES
R2426 T2787 T2788 compound mM,DTT
R2427 T2788 T2784 conj DTT,EGTA
R2428 T2789 T2788 punct ",",DTT
R2429 T2790 T2788 cc and,DTT
R2430 T2791 T2794 nummod 1.0,fluoride
R2431 T2792 T2794 compound mM,fluoride
R2432 T2793 T2794 compound phenylmethylsulfonyl,fluoride
R2433 T2794 T2788 conj fluoride,DTT
R2434 T2795 T2762 punct .,extracted
R2435 T2796 T2797 amod Insoluble,material
R2436 T2797 T2799 nsubjpass material,removed
R2437 T2798 T2799 auxpass was,removed
R2438 T2799 T2799 ROOT removed,removed
R2439 T2800 T2799 agent by,removed
R2440 T2801 T2800 pobj centrifugation,by
R2441 T2802 T2799 prep at,removed
R2442 T2803 T2804 nummod 14000,rpm
R2443 T2804 T2802 pobj rpm,at
R2444 T2805 T2799 punct ",",removed
R2445 T2806 T2799 cc and,removed
R2446 T2807 T2808 det the,supernatant
R2447 T2808 T2814 nsubjpass supernatant,stored
R2448 T2809 T2808 acl containing,supernatant
R2449 T2810 T2812 det the,proteins
R2450 T2811 T2812 amod nuclear,proteins
R2451 T2812 T2809 dobj proteins,containing
R2504 T2865 T2863 conj USA,WI
R2505 T2866 T2857 punct ),system
R2506 T2867 T2852 punct .,performed
R2507 T2868 T2870 compound Consensus,probe
R2508 T2869 T2870 compound oligonucleotide,probe
R2509 T2870 T2879 nsubjpass probe,end-labeled
R2510 T2871 T2870 punct (,probe
R2511 T2872 T2875 nummod 5,AGTTGAGGGGACTTTCCCAGGG-3
R2512 T2873 T2875 punct ′,AGTTGAGGGGACTTTCCCAGGG-3
R2513 T2874 T2875 punct -,AGTTGAGGGGACTTTCCCAGGG-3
R2514 T2875 T2870 appos AGTTGAGGGGACTTTCCCAGGG-3,probe
R2515 T2876 T2875 appos ′,AGTTGAGGGGACTTTCCCAGGG-3
R2516 T2877 T2875 punct ),AGTTGAGGGGACTTTCCCAGGG-3
R2517 T2878 T2879 auxpass was,end-labeled
R2595 T2956 T2959 nmod μL,probe
R2596 T2957 T2959 amod 32P-labled,probe
R2597 T2958 T2959 compound oligonucleotide,probe
R2598 T2959 T2953 pobj probe,of
R2599 T2960 T2964 punct ",",continued
R2600 T2961 T2962 det the,incubation
R2601 T2962 T2964 nsubjpass incubation,continued
R2602 T2963 T2964 auxpass was,continued
R2603 T2964 T2964 ROOT continued,continued
R2604 T2965 T2964 prep for,continued
R2605 T2966 T2967 nummod 20,minutes
R2606 T2967 T2965 pobj minutes,for
R2607 T2968 T2967 prep on,minutes
R2608 T2969 T2968 pobj ice,on
R2609 T2970 T2964 punct .,continued
R2610 T2971 T2973 det The,complexes
R2639 T3000 T2998 pobj °,at
R2640 T3001 T2975 npadvmod C,separated
R2641 T3002 T2975 punct .,separated
R2642 T3003 T3009 prep After,dried
R2643 T3004 T3003 pobj electrophoresis,After
R2644 T3005 T3009 punct ",",dried
R2645 T3006 T3007 det the,gel
R2646 T3007 T3009 nsubjpass gel,dried
R2647 T3008 T3009 auxpass was,dried
R2648 T3009 T3009 ROOT dried,dried
R2649 T3010 T3009 cc and,dried
R2650 T3011 T3009 conj exposed,dried
R2651 T3012 T3011 prep to,exposed
R2652 T3013 T3014 compound X-ray,film
R2653 T3014 T3012 pobj film,to
R2654 T3015 T3017 punct (,Hyperfilm
R2655 T3016 T3017 compound Fuji,Hyperfilm
R2656 T3017 T3014 appos Hyperfilm,film
R2657 T3018 T3017 punct ",",Hyperfilm
R2658 T3019 T3017 npadvmod Tokyo,Hyperfilm
R2659 T3020 T3019 punct ",",Tokyo
R2660 T3021 T3019 conj Japan,Tokyo
R2661 T3022 T3017 punct ),Hyperfilm
R2662 T3023 T3009 punct .,dried
R2663 T3024 T3031 nsubjpass Levels,quantified

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T2673 1749-1756 Binding denotes binding
T2672 1739-1744 Protein denotes NF-κB
T2671 830-850 Protein denotes bovine serum albumin
R2312 T2672 T2673 themeOf NF-κB,binding