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2_test

Id Subject Object Predicate Lexical cue
22529521-8455207-67992085 1577-1578 8455207 denotes 7

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T1878 1782-1789 NN denotes scratch
T4798 8796-8804 NN denotes software
T4797 8791-8795 CD denotes 18.0
T4796 8786-8790 NN denotes SPSS
T4795 8780-8785 VB denotes using
T4794 8777-8779 IN denotes by
T4793 8767-8776 VB denotes performed
T4792 8762-8766 VB denotes were
T4791 8753-8761 NN denotes analyses
T4790 8749-8752 DT denotes All
T4789 8736-8747 JJ denotes significant
T4788 8733-8735 VB denotes be
T4787 8730-8732 TO denotes to
T4786 8719-8729 VB denotes considered
T4785 8714-8718 VB denotes were
T4784 8709-8713 CD denotes 0.05
T4783 8708-8709 SYM denotes <
T4782 8701-8707 NN denotes values
T4781 8699-8700 NN denotes P
T4780 8693-8697 NN denotes test
T4779 8682-8692 NN denotes comparison
T4778 8673-8681 JJ denotes multiple
T4777 8669-8672 NN denotes LSD
T4776 8665-8668 CC denotes and
T4775 8659-8664 NN denotes ANOVA
T4774 8656-8658 IN denotes by
T4773 8646-8655 VB denotes evaluated
T4772 8642-8645 CC denotes and
T4771 8639-8641 NN denotes SD
T4770 8637-8638 SYM denotes ±
T4769 8632-8636 NN denotes mean
T4768 8629-8631 IN denotes as
T4767 8619-8628 VB denotes expressed
T4766 8614-8618 VB denotes were
T4765 8609-8613 NN denotes Data
T4764 8600-8608 NN denotes Analysis
T4763 8588-8599 JJ denotes Statistical
T4762 8583-8586 CD denotes 2.9
T4496 8263-8265 TO denotes to
T4495 8258-8262 NN denotes MMP9
T4494 8254-8257 DT denotes the
T4493 8247-8253 VB denotes enable
T4492 8244-8246 TO denotes to
T4491 8239-8243 NN denotes 37°C
T4490 8236-8238 IN denotes at
T4489 8234-8235 NN denotes h
T4488 8231-8233 CD denotes 40
T4487 8227-8230 IN denotes for
T4486 8220-8226 NN denotes buffer
T4485 8210-8219 NN denotes substrate
T4484 8207-8209 IN denotes in
T4483 8197-8206 VB denotes incubated
T4482 8192-8196 RB denotes then
T4481 8188-8191 CC denotes and
T4480 8182-8187 NN denotes X-100
T4479 8175-8181 NN denotes Triton
T4478 8173-8174 NN denotes %
T4477 8170-8173 CD denotes 2.5
T4476 8167-8169 IN denotes in
T4475 8156-8166 NN denotes incubation
T4474 8153-8155 IN denotes by
T4473 8143-8152 VB denotes renatured
T4472 8138-8142 VB denotes were
T4471 8129-8137 NN denotes proteins
T4470 8125-8128 DT denotes the
T4469 8120-8124 RB denotes Then
T4468 8103-8118 NN denotes electrophoresis
T4467 8100-8102 IN denotes by
T4466 8090-8099 VB denotes separated
T4465 8085-8089 VB denotes were
T4464 8076-8084 NN denotes extracts
T4463 8068-8075 NN denotes protein
T4462 8058-8067 JJ denotes cytosolic
T4461 8055-8057 NN denotes μg
T4460 8052-8054 CD denotes 40
T4459 8050-8051 -COMMA- denotes ,
T4458 8043-8050 RB denotes Briefly
T4457 8040-8041 -RRB- denotes )
T4456 8037-8040 NNP denotes USA
T4455 8035-8036 -COMMA- denotes ,
T4454 8033-8035 NNP denotes MA
T4453 8031-8032 -COMMA- denotes ,
T4452 8028-8031 NNP denotes Inc
T4451 8016-8027 NNP denotes Scientifics
T4450 8009-8015 NNP denotes Genmed
T4449 8008-8009 -LRB- denotes (
T4448 7995-8007 NN denotes instructions
T4447 7992-7994 POS denotes 's
T4446 7980-7992 NN denotes manufacturer
T4445 7976-7979 DT denotes the
T4444 7973-7975 TO denotes to
T4443 7963-7972 VB denotes according
T4442 7953-7962 VB denotes performed
T4441 7949-7952 VB denotes was
T4440 7938-7948 NN denotes zymography
T4439 7930-7937 NN denotes Gelatin
T4438 7923-7928 NN denotes –20°C
T4437 7920-7922 IN denotes at
T4436 7913-7919 VB denotes stored
T4435 7909-7912 CC denotes and
T4434 7894-7908 NN denotes centrifugation
T4433 7891-7893 IN denotes by
T4432 7881-7890 VB denotes separated
T4431 7876-7880 VB denotes were
T4430 7867-7875 NN denotes extracts
T4429 7859-7866 NNP denotes Soluble
T4428 7851-7858 NNP denotes Triton.
T4427 7849-7850 NN denotes %
T4426 7845-7849 CD denotes 0.01
T4425 7841-7844 CC denotes and
T4424 7839-7840 -COMMA- denotes ,
T4423 7835-7839 NN denotes NaN3
T4422 7832-7834 NN denotes mM
T4421 7828-7831 CD denotes 0.2
T4420 7826-7827 -COMMA- denotes ,
T4419 7821-7826 NN denotes CaCl2
T4418 7818-7820 NN denotes mM
T4417 7816-7817 CD denotes 5
T4416 7814-7815 -COMMA- denotes ,
T4415 7810-7814 NN denotes NaCl
T4414 7807-7809 NN denotes mM
T4413 7803-7806 CD denotes 150
T4412 7801-7802 -COMMA- denotes ,
T4411 7800-7801 -RRB- denotes )
T4410 7797-7800 CD denotes 7.4
T4409 7794-7796 NN denotes pH
T4408 7793-7794 -LRB- denotes (
T4407 7784-7792 NN denotes Tris-HCl
T4406 7781-7783 NN denotes mM
T4405 7778-7780 CD denotes 50
T4404 7767-7777 VB denotes containing
T4403 7760-7766 NN denotes buffer
T4402 7754-7759 NN denotes lysis
T4401 7751-7753 IN denotes in
T4400 7739-7750 VB denotes homogenized
T4399 7734-7738 VB denotes were
T4398 7727-7733 NN denotes groups
T4397 7722-7726 CD denotes four
T4396 7719-7721 IN denotes of
T4395 7713-7718 NN denotes Cells
T4394 7702-7712 NN denotes Zymography
T4393 7693-7701 NN denotes Gelatine
T4392 7688-7691 CD denotes 2.8
T4281 7678-7685 NN denotes protein
T4184 7123-7132 VB denotes microwell
T4183 7120-7122 TO denotes to
T4182 7114-7119 VB denotes added
T4181 7109-7113 VB denotes were
T4180 7093-7108 NN denotes biotinconjugate
T4179 7089-7092 CC denotes and
T4178 7081-7088 NN denotes samples
T4177 7076-7080 RB denotes Then
T4176 7069-7074 NN denotes curve
T4175 7060-7068 JJ denotes standard
T4174 7051-7059 VB denotes building
T4173 7047-7050 IN denotes for
T4172 7038-7046 NN denotes dilution
T4171 7029-7037 JJ denotes standard
T4170 7025-7028 DT denotes the
T4169 7017-7024 VB denotes created
T4168 7013-7016 CC denotes and
T4167 7004-7012 NN denotes standard
T4166 7000-7003 DT denotes the
T4165 6991-6999 VB denotes prepared
T4164 6989-6990 -COMMA- denotes ,
T4163 6982-6989 NNP denotes Briefly
T4162 6979-6980 -RRB- denotes )
T4161 6976-6979 NNP denotes USA
T4160 6974-6975 -COMMA- denotes ,
T4159 6972-6974 NNP denotes CA
T4158 6967-6971 NNP denotes Inc.
T4157 6956-6966 NNP denotes MedSystems
T4156 6949-6955 NNP denotes Bender
T4155 6948-6949 -LRB- denotes (
T4154 6935-6947 NN denotes instructions
T4153 6932-6934 POS denotes 's
T4152 6920-6932 NN denotes manufacturer
T4151 6916-6919 DT denotes the
T4150 6913-6915 TO denotes to
T4149 6903-6912 VB denotes according
T4148 6897-6902 NN denotes mouse
T4147 6893-6896 IN denotes for
T4146 6884-6892 JJ denotes specific
T4145 6879-6883 NN denotes kits
T4144 6873-6878 NN denotes ELISA
T4143 6867-6872 VB denotes using
T4142 6856-6866 VB denotes quantified
T4141 6851-6855 VB denotes were
T4140 6843-6850 NN denotes protein
T4139 6841-6842 -RRB- denotes )
T4138 6837-6841 NN denotes IL-6
T4137 6836-6837 -LRB- denotes (
T4136 6822-6835 NN denotes interleukin-6
T4135 6818-6821 CC denotes and
T4134 6816-6817 -COMMA- denotes ,
T4133 6815-6816 -RRB- denotes )
T4132 6810-6815 NN denotes IL-1β
T4131 6809-6810 -LRB- denotes (
T4130 6804-6808 NN denotes beta
T4129 6790-6803 NN denotes interleukin-1
T4128 6788-6789 -COMMA- denotes ,
T4127 6787-6788 -RRB- denotes )
T4126 6782-6787 NN denotes TNF-α
T4125 6781-6782 -LRB- denotes (
T4124 6768-6780 NN denotes factor-alpha
T4123 6759-6767 NN denotes necrosis
T4122 6753-6758 NN denotes tumor
T4121 6750-6752 IN denotes of
T4120 6743-6749 NN denotes Levels
T4119 6740-6741 -RRB- denotes )
T4118 6737-6740 NNP denotes USA
T4117 6735-6736 -COMMA- denotes ,
T4116 6733-6735 NNP denotes IL
T4115 6731-6732 -COMMA- denotes ,
T4114 6725-6731 NNP denotes Pierce
T4113 6724-6725 -LRB- denotes (
T4112 6716-6723 NNP denotes Reagent
T4111 6710-6715 NNP denotes Assay
T4110 6702-6709 NNP denotes Protein
T4109 6697-6701 NNP denotes Plus
T4108 6687-6696 NNP denotes Coomassie
T4107 6684-6686 IN denotes by
T4106 6673-6683 VB denotes determined
T4105 6669-6672 VB denotes was
T4104 6661-6668 NN denotes protein
T4103 6655-6660 JJ denotes total
T4102 6651-6654 CC denotes and
T4101 6649-6650 -COMMA- denotes ,
T4549 8574-8580 NN denotes ImageJ
T4548 8566-8573 NN denotes program
T4547 8557-8565 NN denotes analysis
T4546 8551-8556 NN denotes image
T4545 8546-8550 IN denotes with
T4544 8536-8545 VB denotes performed
T4543 8532-8535 VB denotes was
T4542 8524-8531 NN denotes density
T4541 8519-8523 NN denotes band
T4540 8514-8518 NN denotes MMP9
T4539 8511-8513 IN denotes of
T4538 8496-8510 NN denotes Quantification
T4537 8485-8494 NN denotes substrate
T4536 8481-8484 DT denotes the
T4535 8478-8480 IN denotes of
T4534 8472-8477 NN denotes lysis
T4533 8468-8471 DT denotes the
T4532 8458-8467 VB denotes indicates
T4531 8452-8457 WDT denotes which
T4530 8448-8451 NN denotes gel
T4529 8443-8447 JJ denotes blue
T4528 8440-8442 IN denotes in
T4527 8434-8439 NN denotes bands
T4526 8428-8433 JJ denotes clear
T4525 8425-8427 IN denotes by
T4524 8418-8424 VB denotes showed
T4523 8413-8417 VB denotes were
T4522 8402-8412 NN denotes activities
T4521 8390-8401 JJ denotes Proteolytic
T4520 8380-8388 NN denotes methanol
T4519 8378-8379 NN denotes %
T4518 8376-8378 CD denotes 50
T4517 8373-8375 IN denotes in
T4516 8363-8372 VB denotes destained
T4515 8359-8362 CC denotes and
T4514 8357-8358 NN denotes h
T4513 8355-8356 CD denotes 1
T4512 8351-8354 IN denotes for
T4511 8346-8350 JJ denotes blue
T4510 8336-8345 NNP denotes Coomassie
T4509 8331-8335 IN denotes with
T4508 8323-8330 VB denotes stained
T4507 8319-8322 VB denotes was
T4506 8315-8318 NN denotes gel
T4505 8310-8314 DT denotes each
T4504 8308-8309 -COMMA- denotes ,
T4503 8303-8308 NN denotes water
T4502 8300-8302 IN denotes in
T4501 8292-8299 VB denotes rinsing
T4500 8286-8291 IN denotes After
T4499 8277-8284 NN denotes gelatin
T4498 8273-8276 DT denotes the
T4497 8266-8272 VB denotes cleave
T3671 6504-6512 NN denotes standard
T3670 6495-6503 JJ denotes internal
T3669 6492-6494 IN denotes as
T3668 6487-6491 VB denotes used
T3667 6483-6486 VB denotes was
T3666 6475-6482 NN denotes β-actin
T3665 6472-6474 IN denotes of
T3664 6466-6471 NN denotes level
T3663 6462-6465 DT denotes The
T3662 6455-6460 NN denotes blots
T3661 6451-6454 DT denotes the
T3660 6448-6450 IN denotes of
T3659 6438-6447 NN denotes intensity
T3658 6434-6437 DT denotes the
T3657 6426-6433 VB denotes analyze
T3656 6423-6425 TO denotes to
T3655 6418-6422 VB denotes used
T3654 6414-6417 VB denotes was
T3653 6407-6413 NN denotes ImageJ
T3652 6404-6405 -RRB- denotes )
T3651 6399-6404 NNP denotes Japan
T3650 6397-6398 -COMMA- denotes ,
T3649 6392-6397 NNP denotes Tokyo
T3648 6390-6391 -COMMA- denotes ,
T3647 6381-6390 NNP denotes Hyperfilm
T3646 6376-6380 NNP denotes Fuji
T3645 6375-6376 -LRB- denotes (
T3644 6370-6374 NN denotes film
T3643 6357-6369 JJ denotes radiographic
T3642 6354-6356 TO denotes to
T3641 6346-6353 VB denotes exposed
T3640 6342-6345 CC denotes and
T3639 6340-6341 -RRB- denotes )
T3638 6338-6340 NNP denotes UK
T3637 6336-6337 -COMMA- denotes ,
T3636 6331-6336 NNP denotes Bucks
T3635 6329-6330 -COMMA- denotes ,
T3634 6318-6329 NNP denotes Biosciences
T3633 6309-6317 NNP denotes Amersham
T3632 6308-6309 -LRB- denotes (
T3631 6301-6307 NN denotes system
T3630 6291-6300 NN denotes detection
T3629 6289-6290 -RRB- denotes )
T3628 6286-6289 NN denotes ECL
T3627 6285-6286 -LRB- denotes (
T3626 6267-6284 NN denotes chemiluminescence
T3625 6258-6266 VB denotes enhanced
T3624 6253-6257 IN denotes with
T3623 6243-6252 VB denotes incubated
T3622 6238-6242 VB denotes were
T3621 6232-6237 NN denotes Blots
T3620 6229-6230 NN denotes h
T3619 6227-6228 CD denotes 1
T3618 6223-6226 IN denotes for
T3617 6214-6222 NN denotes dilution
T3616 6209-6213 CD denotes 2000
T3615 6207-6208 -COLON- denotes :
T3614 6205-6206 CD denotes 1
T3613 6203-6204 DT denotes a
T3612 6200-6202 IN denotes at
T3611 6191-6199 NN denotes antibody
T3610 6181-6190 JJ denotes secondary
T3609 6163-6180 JJ denotes peroxidase-linked
T3608 6151-6162 JJ denotes horseradish
T3607 6147-6150 DT denotes the
T3606 6142-6146 IN denotes with
T3605 6134-6141 VB denotes covered
T3604 6130-6133 CC denotes and
T3603 6123-6129 NN denotes buffer
T3602 6115-6122 VB denotes washing
T3601 6111-6114 DT denotes the
T3600 6108-6110 IN denotes in
T3599 6102-6107 NN denotes times
T3598 6096-6101 CD denotes three
T3597 6089-6095 VB denotes washed
T3596 6084-6088 VB denotes were
T3595 6078-6083 NN denotes blots
T3594 6074-6077 DT denotes The
T3593 6061-6072 NN denotes temperature
T3592 6056-6060 NN denotes room
T3591 6053-6055 IN denotes at
T3590 6051-6052 NN denotes h
T3589 6049-6050 CD denotes 1
T3588 6045-6048 IN denotes for
T3587 6039-6044 NN denotes blots
T3586 6035-6038 DT denotes the
T3585 6032-6034 TO denotes to
T3584 6026-6031 VB denotes added
T3583 6021-6025 RB denotes then
T3582 6017-6020 CC denotes and
T3581 6010-6016 NN denotes buffer
T3580 6001-6009 VB denotes blocking
T3579 5998-6000 IN denotes in
T3578 5984-5997 RB denotes appropriately
T3577 5976-5983 VB denotes diluted
T3576 5972-5975 VB denotes was
T3575 5963-5971 NN denotes antibody
T3574 5955-5962 JJ denotes primary
T3573 5950-5954 DT denotes Each
T3572 5947-5948 -RRB- denotes )
T3571 5944-5947 NN denotes kDa
T3570 5941-5943 CD denotes 92
T3569 5939-5940 -COMMA- denotes ,
T3568 5936-5939 NNP denotes USA
T3567 5934-5935 -COMMA- denotes ,
T3566 5932-5934 NNP denotes CA
T3565 5930-5931 -COMMA- denotes ,
T3564 5917-5930 NNP denotes Biotechnology
T3563 5912-5916 NNP denotes Cruz
T3562 5906-5911 NNP denotes Santa
T3561 5904-5905 -COMMA- denotes ,
T3560 5897-5904 NN denotes sc-6841
T3559 5896-5897 -LRB- denotes (
T3558 5894-5895 -RRB- denotes )
T3557 5890-5894 NN denotes MMP9
T3556 5889-5890 -LRB- denotes (
T3555 5887-5888 CD denotes 9
T3554 5870-5886 NN denotes metallopeptidase
T3553 5858-5869 JJ denotes anti-matrix
T3552 5854-5857 CC denotes and
T3551 5852-5853 -RRB- denotes )
T3550 5849-5852 NN denotes kDa
T3549 5846-5848 CD denotes 43
T3548 5844-5845 -COMMA- denotes ,
T3547 5841-5844 NNP denotes USA
T3546 5839-5840 -COMMA- denotes ,
T3545 5837-5839 NNP denotes CA
T3544 5835-5836 -COMMA- denotes ,
T3543 5822-5835 NNP denotes Biotechnology
T3542 5817-5821 NNP denotes Cruz
T3541 5811-5816 NNP denotes Santa
T3540 5809-5810 -COMMA- denotes ,
T3539 5800-5809 NN denotes sc-130657
T3538 5799-5800 -LRB- denotes (
T3537 5786-5798 JJ denotes anti-β-actin
T3536 5784-5785 -COLON- denotes :
T3535 5780-5784 VB denotes used
T3534 5775-5779 VB denotes were
T3533 5764-5774 NN denotes antibodies
T3532 5754-5763 VB denotes following
T3531 5750-5753 DT denotes the
T3530 5746-5749 CC denotes and
T3529 5744-5745 -COMMA- denotes ,
T3528 5741-5744 NN denotes 4°C
T3527 5738-5740 IN denotes at
T3526 5728-5737 RB denotes overnight
T3525 5721-5727 NN denotes buffer
T3524 5714-5720 NN denotes saline
T3523 5711-5713 IN denotes in
T3522 5706-5710 NN denotes milk
T3521 5702-5705 JJ denotes dry
T3520 5695-5701 JJ denotes nonfat
T3519 5693-5694 NN denotes %
T3518 5692-5693 CD denotes 5
T3517 5687-5691 IN denotes with
T3516 5679-5686 VB denotes blocked
T3515 5674-5678 VB denotes were
T4280 7672-7677 JJ denotes total
T4279 7669-7671 IN denotes of
T4278 7663-7668 NN denotes pg/mg
T4277 7660-7662 IN denotes as
T4276 7650-7659 VB denotes expressed
T4275 7646-7649 CC denotes and
T4274 7640-7645 NN denotes curve
T4273 7631-7639 JJ denotes standard
T4272 7627-7630 DT denotes the
T4271 7624-7626 TO denotes to
T4270 7614-7623 VB denotes according
T4269 7603-7613 VB denotes determined
T4268 7599-7602 VB denotes was
T4267 7591-7598 NN denotes protein
T4266 7588-7590 IN denotes of
T4265 7574-7587 NN denotes concentration
T4264 7570-7573 DT denotes The
T4263 7567-7568 -RRB- denotes )
T4262 7564-7567 NNP denotes USA
T4261 7562-7563 -COMMA- denotes ,
T4260 7560-7562 NNP denotes CA
T4259 7558-7559 -COMMA- denotes ,
T4258 7546-7558 NNP denotes Laboratories
T4257 7538-7545 NNP denotes Bio-Rad
T4256 7537-7538 -LRB- denotes (
T4255 7530-7536 NN denotes reader
T4254 7519-7529 NN denotes microplate
T4253 7513-7518 NN denotes ELISA
T4252 7505-7512 JJ denotes Bio-Rad
T4251 7503-7504 DT denotes a
T4250 7497-7502 VB denotes using
T4249 7494-7496 NN denotes nm
T4248 7490-7493 CD denotes 450
T4247 7487-7489 IN denotes at
T4246 7478-7486 VB denotes measured
T4245 7474-7477 VB denotes was
T4244 7464-7473 NN denotes intensity
T4243 7457-7463 NN denotes colour
T4242 7453-7456 DT denotes The
T4241 7446-7451 VB denotes added
T4240 7442-7445 VB denotes was
T4239 7433-7441 NN denotes solution
T4238 7428-7432 NN denotes stop
T4237 7424-7427 DT denotes the
T4236 7422-7423 -COMMA- denotes ,
T4235 7419-7422 NN denotes min
T4234 7413-7418 CD denotes 10–30
T4233 7407-7412 RB denotes about
T4232 7403-7406 IN denotes for
T4231 7393-7402 VB denotes incubated
T4230 7387-7392 IN denotes After
T4229 7376-7385 NN denotes substrate
T4228 7372-7375 NN denotes TMB
T4227 7365-7371 VB denotes adding
T4226 7362-7364 IN denotes by
T4225 7353-7361 VB denotes followed
T4224 7347-7352 NN denotes times
T4223 7345-7346 CD denotes 3
T4222 7338-7344 VB denotes washed
T4221 7333-7337 VB denotes were
T4220 7326-7332 NN denotes strips
T4219 7316-7325 NN denotes microwell
T4218 7314-7315 -COMMA- denotes ,
T4217 7313-7314 NN denotes h
T4216 7311-7312 CD denotes 1
T4215 7307-7310 IN denotes for
T4214 7297-7306 VB denotes incubated
T4213 7291-7296 IN denotes After
T4212 7284-7289 NN denotes wells
T4211 7280-7283 DT denotes all
T4210 7277-7279 TO denotes to
T4209 7271-7276 VB denotes added
T4208 7266-7270 VB denotes were
T4207 7249-7265 NN denotes streptavidin-HRP
T4206 7245-7248 CC denotes and
T4205 7243-7244 -COMMA- denotes ,
T4204 7237-7243 NN denotes buffer
T4203 7232-7236 NN denotes wash
T4202 7227-7231 IN denotes with
T4201 7221-7226 NN denotes times
T4200 7219-7220 CD denotes 3
T4199 7212-7218 VB denotes washed
T4198 7207-7211 VB denotes were
T4197 7200-7206 NN denotes strips
T4196 7190-7199 NN denotes microwell
T4195 7186-7189 DT denotes the
T4194 7184-7185 -COMMA- denotes ,
T4193 7173-7184 NN denotes temperature
T4192 7168-7172 NN denotes room
T4191 7165-7167 IN denotes at
T4190 7163-7164 NN denotes h
T4189 7161-7162 CD denotes 2
T4188 7157-7160 IN denotes for
T4187 7147-7156 VB denotes incubated
T4186 7141-7146 IN denotes After
T4185 7133-7139 NN denotes strips
T3514 5664-5673 NN denotes membranes
T3513 5662-5663 -COMMA- denotes ,
T3512 5648-5662 NN denotes immunoblotting
T3511 5644-5647 IN denotes For
T3510 5634-5642 NN denotes membrane
T3509 5619-5633 NN denotes nitrocellulose
T3508 5617-5618 DT denotes a
T3507 5612-5616 IN denotes onto
T3506 5597-5611 VB denotes electroblotted
T3505 5593-5596 CC denotes and
T3504 5591-5592 -COMMA- denotes ,
T3503 5576-5591 NN denotes electrophoresis
T3502 5572-5575 NN denotes gel
T3501 5557-5571 NN denotes polyacrylamide
T3500 5549-5556 NN denotes sulfate
T3499 5541-5548 NN denotes dodecyl
T3498 5534-5540 NN denotes sodium
T3497 5532-5533 NN denotes %
T3496 5530-5532 CD denotes 10
T3495 5527-5529 IN denotes by
T3494 5517-5526 VB denotes separated
T3493 5515-5516 -COMMA- denotes ,
T3492 5509-5515 NN denotes buffer
T3491 5501-5508 NN denotes loading
T3490 5494-5500 NN denotes sample
T3489 5486-5493 NNP denotes Laemmli
T3221 4996-5004 NN denotes standard
T3220 4987-4995 JJ denotes internal
T3219 4984-4986 DT denotes an
T3218 4981-4983 IN denotes as
T3217 4976-4980 VB denotes used
T3216 4972-4975 VB denotes was
T3215 4964-4971 NN denotes β-actin
T3214 4961-4963 IN denotes of
T3213 4955-4960 NN denotes level
T3212 4951-4954 DT denotes The
T3211 4942-4949 NN denotes program
T3210 4935-4941 NN denotes ImageJ
T3209 4932-4934 IN denotes by
T3208 4923-4931 VB denotes analyzed
T3207 4919-4922 VB denotes was
T3206 4913-4918 NN denotes bands
T3205 4909-4912 DT denotes the
T3204 4906-4908 IN denotes of
T3203 4896-4905 NN denotes intensity
T3202 4892-4895 DT denotes The
T3201 4875-4890 NN denotes electrophoresis
T3200 4871-4874 NN denotes gel
T3199 4863-4870 NN denotes agarose
T3198 4860-4862 IN denotes by
T3197 4851-4859 VB denotes detected
T3196 4846-4850 VB denotes were
T3195 4837-4845 NN denotes products
T3194 4833-4836 NN denotes PCR
T3193 4821-4831 NN denotes parameters
T3192 4817-4820 NN denotes PCR
T3191 4813-4816 CC denotes and
T3190 4805-4812 NN denotes primers
T3189 4801-4804 DT denotes the
T3188 4795-4800 VB denotes shows
T3187 4793-4794 CD denotes 1
T3186 4787-4792 JJ denotes Table
T3185 4776-4784 NN denotes protocol
T3184 4773-4775 POS denotes 's
T3183 4761-4773 NN denotes manufacturer
T3182 4757-4760 DT denotes the
T3181 4754-4756 TO denotes to
T3180 4744-4753 VB denotes according
T3179 4742-4743 -RRB- denotes )
T3178 4739-4742 NNP denotes USA
T3177 4737-4738 -COMMA- denotes ,
T3176 4735-4737 NNP denotes WI
T3175 4733-4734 -COMMA- denotes ,
T3174 4726-4733 NNP denotes Promega
T3173 4725-4726 -LRB- denotes (
T3172 4721-4724 NNP denotes Mix
T3171 4714-4720 NNP denotes Master
T3170 4708-4713 NNP denotes Green
T3169 4702-4707 NNP denotes GoTaq
T3168 4697-4701 IN denotes with
T3167 4687-4696 VB denotes conducted
T3166 4683-4686 VB denotes was
T3165 4669-4682 NN denotes transcription
T3164 4661-4668 JJ denotes Reverse
T3163 4654-4659 NN denotes −20°C
T3162 4651-4653 IN denotes in
T3161 4644-4650 VB denotes stored
T3160 4640-4643 VB denotes was
T3159 4635-4639 NN denotes cDNA
T3158 4631-4634 DT denotes The
T3157 4621-4629 NN denotes protocol
T3156 4618-4620 POS denotes 's
T3155 4606-4618 NN denotes manufacturer
T3154 4602-4605 DT denotes the
T3153 4599-4601 TO denotes to
T3152 4589-4598 VB denotes according
T3151 4587-4588 -RRB- denotes )
T3150 4582-4587 NNP denotes China
T3149 4580-4581 -COMMA- denotes ,
T3148 4573-4580 NNP denotes Jiangsu
T3147 4571-4572 -COMMA- denotes ,
T3146 4563-4571 NNP denotes Biunique
T3145 4562-4563 -LRB- denotes (
T3144 4555-4561 NN denotes RT-Kit
T3143 4545-4554 JJ denotes BU-Script
T3142 4540-4544 IN denotes with
T3141 4536-4539 NN denotes RNA
T3140 4530-4535 JJ denotes total
T3139 4527-4529 IN denotes of
T3138 4524-4526 NN denotes μg
T3137 4522-4523 CD denotes 2
T3136 4517-4521 IN denotes from
T3135 4505-4516 VB denotes synthesized
T3134 4501-4504 VB denotes was
T3133 4496-4500 NN denotes cDNA
T3132 4480-4495 JJ denotes single-stranded
T3131 4476-4479 CC denotes and
T3130 4474-4475 -COMMA- denotes ,
T3129 4473-4474 -RRB- denotes )
T3128 4470-4473 NNP denotes USA
T3127 4468-4469 -COMMA- denotes ,
T3126 4466-4468 NNP denotes CA
T3125 4464-4465 -COMMA- denotes ,
T3124 4454-4464 NN denotes Invitrogen
T3123 4453-4454 -LRB- denotes (
T3122 4446-4452 NN denotes Trizol
T3121 4441-4445 IN denotes with
T3120 4432-4440 VB denotes isolated
T3119 4428-4431 VB denotes was
T3118 4424-4427 NN denotes RNA
T3117 4418-4423 JJ denotes Total
T3116 4411-4417 NN denotes RT-PCR
T3115 4406-4409 CD denotes 2.5
T3479 5408-5418 NN denotes micrograms
T3478 5402-5407 CD denotes Fifty
T3477 5399-5400 -RRB- denotes )
T3476 5396-5399 NNP denotes USA
T3475 5394-5395 -COMMA- denotes ,
T3474 5392-5394 NNP denotes IL
T3473 5390-5391 -COMMA- denotes ,
T3472 5384-5390 NNP denotes Pierce
T3471 5383-5384 -LRB- denotes (
T3470 5375-5382 NNP denotes Reagent
T3469 5369-5374 NNP denotes Assay
T3468 5361-5368 NNP denotes Protein
T3467 5356-5360 NNP denotes Plus
T3466 5346-5355 NNP denotes Coomassie
T3465 5343-5345 IN denotes by
T3464 5333-5342 VB denotes estimated
T3463 5328-5332 VB denotes were
T3462 5313-5327 NN denotes concentrations
T3461 5305-5312 NN denotes Protein
T3460 5300-5303 NN denotes 4°C
T3459 5297-5299 IN denotes at
T3458 5293-5296 NN denotes min
T3457 5290-5292 CD denotes 15
T3456 5286-5289 IN denotes for
T3455 5284-5285 NN denotes g
T3454 5277-5283 CD denotes 12,000
T3453 5274-5276 IN denotes at
T3452 5262-5273 VB denotes centrifuged
T3451 5258-5261 CC denotes and
T3450 5256-5257 -RRB- denotes )
T3449 5253-5256 CD denotes 7.4
T3448 5250-5252 NN denotes pH
T3447 5246-5249 NNP denotes PBS
T3446 5243-5245 IN denotes in
T3445 5234-5242 NN denotes cocktail
T3444 5224-5233 NN denotes inhibitor
T3443 5215-5223 NN denotes protease
T3442 5211-5214 CC denotes and
T3441 5209-5210 -COMMA- denotes ,
T3440 5205-5209 NN denotes PMSF
T3439 5202-5204 NN denotes mM
T3438 5200-5201 CD denotes 1
T3437 5198-5199 -COMMA- denotes ,
T3436 5195-5198 NN denotes DTT
T3435 5192-5194 NN denotes mM
T3434 5188-5191 CD denotes 0.5
T3433 5186-5187 -COMMA- denotes ,
T3432 5183-5186 NN denotes NaF
T3431 5180-5182 NN denotes mM
T3430 5177-5179 CD denotes 20
T3429 5175-5176 -COMMA- denotes ,
T3428 5169-5175 NN denotes Na3VO4
T3427 5166-5168 NN denotes mM
T3426 5164-5165 CD denotes 1
T3425 5162-5163 -COMMA- denotes ,
T3424 5158-5162 NN denotes EGTA
T3423 5155-5157 NN denotes mM
T3422 5153-5154 CD denotes 1
T3421 5151-5152 -COMMA- denotes ,
T3420 5147-5151 NN denotes EDTA
T3419 5144-5146 NN denotes mM
T3418 5142-5143 CD denotes 1
T3417 5140-5141 -COMMA- denotes ,
T3416 5137-5140 NN denotes SDS
T3415 5135-5136 NN denotes %
T3414 5132-5135 CD denotes 0.1
T3413 5130-5131 -COMMA- denotes ,
T3412 5118-5130 NN denotes deoxycholate
T3411 5111-5117 NN denotes sodium
T3410 5109-5110 NN denotes %
T3409 5106-5109 CD denotes 0.5
T3408 5104-5105 -COMMA- denotes ,
T3407 5100-5104 NN denotes NP40
T3406 5098-5099 NN denotes %
T3405 5097-5098 CD denotes 1
T3404 5096-5097 -LRB- denotes (
T3403 5089-5095 NN denotes buffer
T3402 5084-5088 NN denotes RIPA
T3401 5081-5083 IN denotes in
T3400 5069-5080 VB denotes homogenized
T3399 5064-5068 VB denotes were
T3398 5058-5063 NN denotes cells
T3397 5056-5057 -COMMA- denotes ,
T3396 5049-5056 NN denotes lysates
T3395 5041-5048 NN denotes protein
T3394 5035-5040 JJ denotes total
T3393 5028-5034 VB denotes obtain
T3392 5025-5027 TO denotes To
T3391 5020-5024 NNP denotes Blot
T3390 5012-5019 NNP denotes Western
T3389 5007-5010 CD denotes 2.6
T2662 4397-4403 NN denotes ImageJ
T2661 4388-4396 NN denotes software
T2660 4385-4387 IN denotes by
T2659 4374-4384 VB denotes quantified
T2658 4369-4373 VB denotes were
T2657 4360-4368 NN denotes activity
T2656 4352-4359 NN denotes binding
T2655 4348-4351 NN denotes DNA
T2654 4342-4347 NN denotes NF-κB
T2653 4339-4341 IN denotes of
T2652 4332-4338 NN denotes Levels
T2651 4329-4330 -RRB- denotes )
T2650 4324-4329 NNP denotes Japan
T2649 4322-4323 -COMMA- denotes ,
T2648 4317-4322 NNP denotes Tokyo
T2647 4315-4316 -COMMA- denotes ,
T2646 4306-4315 NNP denotes Hyperfilm
T2645 4301-4305 NNP denotes Fuji
T2644 4300-4301 -LRB- denotes (
T2643 4295-4299 NN denotes film
T2642 4289-4294 NN denotes X-ray
T2641 4286-4288 TO denotes to
T2640 4278-4285 VB denotes exposed
T2639 4274-4277 CC denotes and
T2638 4268-4273 VB denotes dried
T2637 4264-4267 VB denotes was
T2636 4260-4263 NN denotes gel
T2635 4256-4259 DT denotes the
T2634 4254-4255 -COMMA- denotes ,
T2633 4239-4254 NN denotes electrophoresis
T2632 4233-4238 IN denotes After
T2631 4228-4231 NN denotes 4°C
T2630 4225-4227 IN denotes at
T2629 4220-4224 NN denotes hour
T2628 4218-4219 CD denotes 1
T2627 4214-4217 IN denotes for
T2626 4212-4213 NN denotes V
T2625 4208-4211 CD denotes 390
T2624 4205-4207 IN denotes at
T2623 4203-4204 -RRB- denotes )
T2622 4187-4203 NN denotes tris-borate-EDTA
T2621 4186-4187 -LRB- denotes (
T2620 4179-4185 NN denotes buffer
T2619 4175-4178 NN denotes TBE
T2618 4173-4174 SYM denotes ×
T2617 4169-4172 CD denotes 0.5
T2616 4166-4168 IN denotes in
T2615 4162-4165 NN denotes gel
T2614 4147-4161 NN denotes polyacrylamide
T2613 4133-4146 JJ denotes nondenaturing
T2612 4131-4132 NN denotes %
T2611 4130-4131 CD denotes 4
T2610 4127-4129 IN denotes on
T2609 4111-4126 NN denotes electrophoresis
T2608 4108-4110 IN denotes by
T2607 4098-4107 VB denotes separated
T2606 4093-4097 VB denotes were
T2605 4083-4092 NN denotes complexes
T2604 4071-4082 JJ denotes DNA-protein
T2603 4067-4070 DT denotes The
T2602 4062-4065 NN denotes ice
T2601 4059-4061 IN denotes on
T2600 4051-4058 NN denotes minutes
T2599 4048-4050 CD denotes 20
T2598 4044-4047 IN denotes for
T2597 4034-4043 VB denotes continued
T2596 4030-4033 VB denotes was
T2595 4019-4029 NN denotes incubation
T2594 4015-4018 DT denotes the
T2593 4013-4014 -COMMA- denotes ,
T2592 4008-4013 NN denotes probe
T2591 3992-4007 NN denotes oligonucleotide
T2590 3981-3991 JJ denotes 32P-labled
T2589 3978-3980 NN denotes μL
T2588 3976-3977 CD denotes 1
T2587 3972-3975 DT denotes the
T2586 3969-3971 IN denotes of
T2585 3960-3968 NN denotes addition
T2584 3954-3959 IN denotes After
T2583 3949-3952 NN denotes ice
T3488 5483-5485 IN denotes in
T3487 5468-5482 JJ denotes heat-denatured
T3486 5463-5467 VB denotes were
T3485 5454-5462 NN denotes extracts
T3484 5446-5453 NN denotes protein
T3483 5436-5445 JJ denotes cytosolic
T3482 5426-5435 VB denotes resulting
T3481 5422-5425 DT denotes the
T3480 5419-5421 IN denotes of
T2582 3946-3948 IN denotes on
T2581 3938-3945 NN denotes minutes
T2580 3935-3937 CD denotes 20
T2579 3931-3934 IN denotes for
T2578 3925-3930 NN denotes dI-dC
T2577 3920-3924 NN denotes poly
T2576 3917-3919 NN denotes μg
T2575 3915-3916 CD denotes 2
T2574 3911-3914 CC denotes and
T2573 3909-3910 -COMMA- denotes ,
T2572 3906-3909 NN denotes DTT
T2571 3903-3905 NN denotes mM
T2570 3899-3902 CD denotes 0.5
T2569 3897-3898 -COMMA- denotes ,
T2568 3893-3897 NN denotes EDTA
T2567 3890-3892 NN denotes mM
T2566 3886-3889 CD denotes 0.5
T2565 3884-3885 -COMMA- denotes ,
T2564 3876-3884 NN denotes glycerol
T2563 3874-3875 NN denotes %
T2562 3873-3874 CD denotes 4
T2561 3871-3872 -COMMA- denotes ,
T2560 3867-3871 NN denotes NaCl
T2559 3864-3866 NN denotes mM
T2558 3860-3863 CD denotes 0.5
T2557 3858-3859 -COMMA- denotes ,
T2493 3531-3536 VB denotes using
T2492 3521-3530 VB denotes performed
T2491 3517-3520 VB denotes was
T2490 3512-3516 NN denotes EMSA
T2489 3509-3510 -RRB- denotes )
T2488 3506-3509 NNP denotes USA
T2487 3504-3505 -COMMA- denotes ,
T2486 3502-3504 NNP denotes IL
T2485 3500-3501 -COMMA- denotes ,
T2484 3492-3500 NNP denotes Rockford
T2483 3490-3491 -COMMA- denotes ,
T2482 3478-3490 NNP denotes Biochemicals
T2481 3471-3477 NNP denotes Pierce
T2480 3470-3471 -LRB- denotes (
T2479 3461-3469 JJ denotes standard
T2478 3457-3460 DT denotes the
T2477 3454-3456 IN denotes as
T2476 3446-3453 NN denotes albumin
T2475 3440-3445 NN denotes serum
T2474 3433-3439 JJ denotes bovine
T2473 3428-3432 IN denotes with
T2472 3424-3427 NN denotes kit
T2471 3418-3423 NN denotes assay
T2470 3413-3417 NN denotes acid
T2469 3399-3412 JJ denotes bicinchoninic
T2468 3397-3398 DT denotes a
T2467 3391-3396 VB denotes using
T2466 3380-3390 VB denotes determined
T2465 3376-3379 VB denotes was
T2464 3362-3375 NN denotes concentration
T2463 3354-3361 NN denotes Protein
T2462 3349-3352 NN denotes use
T2461 3343-3348 IN denotes until
T2460 3337-3342 NN denotes –80°C
T2459 3334-3336 IN denotes at
T2458 3327-3333 VB denotes stored
T2457 3323-3326 VB denotes was
T2456 3314-3322 NN denotes proteins
T2455 3306-3313 JJ denotes nuclear
T2454 3302-3305 DT denotes the
T2453 3291-3301 VB denotes containing
T2452 3279-3290 NN denotes supernatant
T2451 3275-3278 DT denotes the
T2450 3271-3274 CC denotes and
T2449 3269-3270 -COMMA- denotes ,
T2448 3266-3269 NN denotes rpm
T2447 3260-3265 CD denotes 14000
T2446 3257-3259 IN denotes at
T2445 3242-3256 NN denotes centrifugation
T2444 3239-3241 IN denotes by
T2443 3231-3238 VB denotes removed
T2442 3227-3230 VB denotes was
T2441 3218-3226 NN denotes material
T2440 3208-3217 JJ denotes Insoluble
T2439 3198-3206 NN denotes fluoride
T2438 3177-3197 NN denotes phenylmethylsulfonyl
T2437 3174-3176 NN denotes mM
T2436 3170-3173 CD denotes 1.0
T2435 3166-3169 CC denotes and
T2434 3164-3165 -COMMA- denotes ,
T2433 3161-3164 NN denotes DTT
T2432 3158-3160 NN denotes mM
T2431 3154-3157 CD denotes 1.0
T2430 3152-3153 -COMMA- denotes ,
T2429 3148-3152 NN denotes EGTA
T2428 3145-3147 NN denotes mM
T2427 3141-3144 CD denotes 1.0
T2426 3139-3140 -COMMA- denotes ,
T2425 3135-3139 NN denotes EDTA
T2424 3132-3134 NN denotes mM
T2423 3128-3131 CD denotes 1.0
T2422 3126-3127 -COMMA- denotes ,
T2421 3122-3126 NN denotes NaCl
T2420 3119-3121 NN denotes mM
T2419 3115-3118 CD denotes 0.4
T2418 3113-3114 -COMMA- denotes ,
T2417 3112-3113 -RRB- denotes )
T2416 3109-3112 CD denotes 7.9
T2415 3106-3108 NN denotes pH
T2414 3105-3106 -LRB- denotes (
T2413 3099-3104 NN denotes HEPES
T2412 3096-3098 NN denotes mM
T2411 3093-3095 CD denotes 20
T2410 3082-3092 VB denotes containing
T2409 3075-3081 NN denotes buffer
T2408 3070-3074 IN denotes with
T2407 3060-3069 VB denotes extracted
T2406 3055-3059 VB denotes were
T2405 3046-3054 NN denotes proteins
T2404 3038-3045 JJ denotes nuclear
T2403 3034-3037 DT denotes The
T2402 3018-3032 NN denotes centrifugation
T2401 3015-3017 IN denotes by
T2400 3006-3014 VB denotes pelleted
T2399 3001-3005 VB denotes were
T2398 2994-3000 NN denotes nuclei
T2397 2990-2993 DT denotes the
T2396 2986-2989 CC denotes and
T2395 2984-2985 -COMMA- denotes ,
T2394 2977-2984 NN denotes briefly
T2393 2968-2976 VB denotes vortexed
T2392 2964-2967 CC denotes and
T2391 2958-2963 VB denotes added
T2390 2954-2957 VB denotes was
T2389 2949-2953 NN denotes P-40
T2388 2941-2948 NN denotes Nonidet
T2387 2939-2940 NN denotes %
T2386 2937-2939 CD denotes 10
T2385 2932-2936 RB denotes Then
T2384 2922-2930 NN denotes fluoride
T2383 2901-2921 NN denotes phenylmethylsulfonyl
T2382 2898-2900 NN denotes mM
T2381 2894-2897 CD denotes 0.5
T2380 2890-2893 CC denotes and
T2379 2888-2889 -COMMA- denotes ,
T2378 2885-2888 NN denotes DTT
T2377 2882-2884 NN denotes mM
T2376 2878-2881 CD denotes 1.0
T2375 2876-2877 -COMMA- denotes ,
T2374 2872-2876 NN denotes EGTA
T2373 2869-2871 NN denotes mM
T2372 2865-2868 CD denotes 0.1
T2371 2863-2864 -COMMA- denotes ,
T2370 2859-2863 NN denotes EDTA
T2369 2856-2858 NN denotes mM
T2368 2852-2855 CD denotes 0.1
T2367 2850-2851 -COMMA- denotes ,
T2366 2847-2850 NN denotes KCl
T2365 2844-2846 NN denotes mM
T2364 2841-2843 CD denotes 10
T2363 2839-2840 -COMMA- denotes ,
T2362 2838-2839 -RRB- denotes )
T2361 2835-2838 CD denotes 7.9
T2360 2832-2834 NN denotes pH
T2359 2831-2832 -LRB- denotes (
T2358 2825-2830 NN denotes HEPES
T2357 2822-2824 NN denotes mM
T2356 2819-2821 CD denotes 10
T2355 2808-2818 VB denotes containing
T2354 2801-2807 NN denotes buffer
T2353 2798-2800 IN denotes in
T2352 2786-2797 VB denotes resuspended
T2351 2782-2785 VB denotes was
T2350 2767-2781 NN denotes centrifugation
T2349 2764-2766 IN denotes by
T2348 2755-2763 VB denotes obtained
T2347 2748-2754 NN denotes pellet
T2346 2743-2747 NN denotes cell
T2345 2739-2742 DT denotes The
T2344 2734-2737 NNP denotes PBS
T2343 2729-2733 JJ denotes cold
T2342 2724-2728 IN denotes into
T2341 2715-2723 VB denotes scraping
T2340 2712-2714 IN denotes by
T2339 2702-2711 VB denotes harvested
T2338 2698-2701 CC denotes and
T2337 2694-2697 NNP denotes PBS
T2336 2689-2693 IN denotes with
T2335 2682-2688 VB denotes washed
T2334 2677-2681 VB denotes were
T2333 2666-2676 NN denotes astrocytes
T2332 2663-2665 IN denotes of
T2331 2652-2662 NN denotes Monolayers
T2330 2650-2651 -RRB- denotes )
T2329 2646-2650 NN denotes EMSA
T2328 2645-2646 -LRB- denotes (
T2327 2639-2644 NN denotes Assay
T2326 2633-2638 NN denotes Shift
T2325 2624-2632 NN denotes Mobility
T2324 2608-2623 JJ denotes Electrophoretic
T2323 2603-2606 CD denotes 2.4
T2162 2598-2600 NN denotes SD
T2161 2596-2597 SYM denotes ±
T2160 2593-2595 NN denotes nm
T2159 2589-2592 CD denotes 450
T2158 2586-2588 IN denotes at
T2157 2580-2585 NN denotes value
T2156 2577-2579 NN denotes OD
T2155 2574-2576 IN denotes of
T2154 2569-2573 NN denotes mean
T2153 2566-2568 IN denotes as
T2152 2556-2565 VB denotes expressed
T2151 2551-2555 VB denotes were
T2150 2543-2550 NN denotes Results
T2149 2529-2541 NN denotes sextuplicate
T2148 2526-2528 IN denotes in
T2147 2516-2525 VB denotes performed
T2146 2511-2515 VB denotes were
T2145 2498-2510 NN denotes measurements
T2144 2494-2497 DT denotes All
T2143 2491-2492 -RRB- denotes )
T2142 2488-2491 NNP denotes USA
T2141 2486-2487 -COMMA- denotes ,
T2140 2484-2486 NNP denotes CA
T2139 2482-2483 -COMMA- denotes ,
T2138 2470-2482 NNP denotes Laboratories
T2137 2462-2469 NNP denotes Bio-Rad
T2136 2461-2462 -LRB- denotes (
T2135 2454-2460 NN denotes reader
T2134 2443-2453 NN denotes microplate
T2133 2437-2442 NN denotes ELISA
T2132 2429-2436 JJ denotes Bio-Rad
T2131 2427-2428 DT denotes a
T2130 2421-2426 VB denotes using
T2129 2418-2420 NN denotes nm
T2128 2414-2417 CD denotes 450
T2127 2411-2413 IN denotes at
T2126 2406-2410 VB denotes read
T2125 2402-2405 VB denotes was
T2124 2396-2401 NN denotes value
T2123 2393-2395 NN denotes OD
T2122 2388-2392 RB denotes Then
T2121 2385-2386 NN denotes h
T2120 2383-2384 CD denotes 1
T2119 2379-2382 IN denotes for
T2118 2369-2378 VB denotes incubated
T2117 2365-2368 CC denotes and
T2116 2360-2364 RB denotes well
T2115 2354-2359 DT denotes every
T2114 2349-2353 IN denotes into
T2113 2343-2348 VB denotes added
T2112 2339-2342 VB denotes was
T2111 2333-2338 NN denotes CCK-8
T2110 2330-2332 NN denotes μL
T2109 2327-2329 CD denotes 10
T2108 2325-2326 -COMMA- denotes ,
T2107 2318-2325 NNP denotes Briefly
T2106 2308-2316 NN denotes protocol
T2105 2305-2307 POS denotes 's
T2104 2293-2305 NN denotes manufacturer
T2103 2289-2292 DT denotes the
T2102 2286-2288 TO denotes to
T2101 2276-2285 VB denotes according
T2100 2268-2275 NN denotes scratch
T2099 2262-2267 IN denotes after
T2098 2260-2261 NN denotes h
T2097 2257-2259 CD denotes 24
T2096 2251-2256 NN denotes assay
T2095 2249-2250 -RRB- denotes )
T2094 2244-2249 NNP denotes Japan
T2093 2242-2243 -COMMA- denotes ,
T2092 2235-2242 NNP denotes Dojindo
T2091 2234-2235 -LRB- denotes (
T2090 2232-2233 -RRB- denotes )
T2089 2227-2232 NN denotes CCK-8
T2088 2226-2227 -LRB- denotes (
T2087 2220-2225 NN denotes kit-8
T2086 2211-2219 NN denotes counting
T2085 2206-2210 NN denotes cell
T2084 2203-2205 IN denotes by
T2083 2194-2202 VB denotes assessed
T2082 2190-2193 VB denotes was
T2081 2184-2189 NN denotes death
T2080 2179-2183 NN denotes cell
T2079 2169-2178 NN denotes Astrocyte
T2078 2162-2167 IN denotes above
T2077 2152-2161 VB denotes mentioned
T2076 2149-2151 IN denotes as
T2075 2141-2148 VB denotes scrated
T2074 2137-2140 VB denotes was
T2073 2132-2136 NN denotes cell
T2072 2128-2131 DT denotes the
T2071 2123-2127 RB denotes Then
T2556 3853-3858 NN denotes MgCl2
T2555 3850-3852 NN denotes mM
T2554 3848-3849 CD denotes 1
T2553 3846-3847 -COMMA- denotes ,
T2552 3845-3846 -RRB- denotes )
T2551 3842-3845 CD denotes 7.5
T2550 3839-3841 NN denotes PH
T2549 3838-3839 -LRB- denotes (
T2548 3829-3837 NN denotes Tris-HCl
T2547 3826-3828 NN denotes mM
T2546 3823-3825 CD denotes 10
T2545 3812-3822 VB denotes containing
T2544 3805-3811 NN denotes buffer
T2543 3797-3804 NN denotes binding
T2542 3794-3796 NN denotes μL
T2541 3791-3793 CD denotes 20
T2540 3788-3790 IN denotes in
T2539 3775-3787 VB denotes preincubated
T2538 3771-3774 VB denotes was
T2537 3769-3770 -RRB- denotes )
T2536 3767-3769 NN denotes μg
T2535 3764-3766 CD denotes 20
T2534 3763-3764 -LRB- denotes (
T2533 3755-3762 NN denotes protein
T2532 3747-3754 JJ denotes Nuclear
T2531 3744-3745 -RRB- denotes )
T2530 3739-3744 NNP denotes China
T2529 3737-3738 -COMMA- denotes ,
T2528 3730-3737 NNP denotes Beijing
T2527 3728-3729 -COMMA- denotes ,
T2526 3720-3728 NNP denotes Biotech.
T2525 3715-3719 NNP denotes Free
T2524 3714-3715 -LRB- denotes (
T2523 3709-3713 NN denotes -ATP
T2522 3708-3709 -RRB- denotes ]
T2521 3703-3708 NN denotes γ-32P
T2520 3702-3703 -LRB- denotes [
T2519 3696-3701 VB denotes using
T2518 3689-3695 NN denotes kinase
T2517 3671-3688 NN denotes T4-polynucleotide
T2516 3668-3670 IN denotes by
T2515 3656-3667 VB denotes end-labeled
T2514 3652-3655 VB denotes was
T2513 3650-3651 -RRB- denotes )
T2512 3649-3650 -DQE- denotes ′
T2511 3622-3649 NN denotes 5′-AGTTGAGGGGACTTTCCCAGGG-3
T2510 3621-3622 -LRB- denotes (
T2509 3615-3620 NN denotes probe
T2508 3599-3614 NN denotes oligonucleotide
T2507 3589-3598 NNP denotes Consensus
T2506 3586-3587 -RRB- denotes )
T2505 3583-3586 NNP denotes USA
T2504 3581-3582 -COMMA- denotes ,
T2503 3579-3581 NNP denotes WI
T2502 3577-3578 -COMMA- denotes ,
T2501 3570-3577 NNP denotes Madison
T2500 3568-3569 -COMMA- denotes ,
T2499 3561-3568 NNP denotes Promega
T2498 3560-3561 -LRB- denotes (
T2497 3553-3559 NN denotes system
T2496 3547-3552 NN denotes assay
T2495 3541-3546 NN denotes shift
T2494 3537-3540 NN denotes gel
T2070 2115-2121 VB denotes adhere
T2069 2112-2114 TO denotes to
T2068 2110-2111 NN denotes h
T2067 2107-2109 CD denotes 24
T2066 2103-2106 IN denotes for
T2065 2092-2102 VB denotes cultivated
T2064 2088-2091 CC denotes and
T2063 2081-2087 NN denotes plates
T2062 2073-2080 NN denotes culture
T2061 2065-2072 JJ denotes 96-well
T2060 2062-2064 TO denotes to
T2059 2055-2061 VB denotes seeded
T2058 2050-2054 VB denotes were
T2057 2044-2049 NN denotes cells
T2056 2035-2043 NN denotes 103/well
T2055 2033-2034 SYM denotes ×
T2054 2031-2032 CD denotes 1
T2053 2025-2030 NN denotes Assay
T2052 2019-2024 NN denotes CCK-8
T2051 2016-2018 IN denotes by
T2050 2004-2015 NNP denotes Measurement
T2049 1998-2003 NNP denotes Death
T2048 1993-1997 NNP denotes Cell
T2047 1988-1991 CD denotes 2.3
T1499 1446-1453 NN denotes studies
T1498 1436-1445 VB denotes following
T1497 1432-1435 DT denotes the
T1496 1429-1431 IN denotes in
T1495 1424-1428 VB denotes used
T1494 1419-1423 VB denotes were
T1493 1407-1418 NN denotes generations
T1492 1403-1406 CD denotes 2-3
T1491 1399-1402 IN denotes for
T1490 1390-1398 VB denotes passaged
T1489 1384-1389 NN denotes Cells
T1488 1381-1382 -RRB- denotes )
T1487 1378-1381 NNP denotes USA
T1486 1376-1377 -COMMA- denotes ,
T1485 1374-1376 NNP denotes CA
T1484 1372-1373 -COMMA- denotes ,
T1483 1359-1372 NNP denotes Biotechnology
T1482 1354-1358 NNP denotes Cruz
T1481 1348-1353 NNP denotes Santa
T1480 1346-1347 -COMMA- denotes ,
T1479 1337-1346 NN denotes sc-166481
T1478 1336-1337 -LRB- denotes (
T1477 1331-1335 NN denotes GFAP
T1476 1327-1330 IN denotes for
T1475 1310-1326 NN denotes immunoreactivity
T1474 1307-1309 IN denotes by
T1473 1296-1306 VB denotes determined
T1472 1291-1295 VB denotes were
T1471 1285-1290 WDT denotes which
T1470 1283-1284 -COMMA- denotes ,
T1469 1273-1283 NN denotes astrocytes
T1468 1266-1272 RB denotes mainly
T1467 1261-1265 VB denotes were
T1466 1252-1260 NN denotes cultures
T1465 1242-1251 VB denotes resulting
T1464 1238-1241 DT denotes the
T1463 1236-1237 -COMMA- denotes ,
T1462 1229-1236 VB denotes shaking
T1461 1223-1228 IN denotes After
T1460 1213-1221 NN denotes cultures
T1459 1209-1212 DT denotes the
T1458 1204-1208 IN denotes from
T1457 1198-1203 NN denotes cells
T1456 1189-1197 JJ denotes adherent
T1455 1184-1188 RB denotes less
T1454 1180-1183 CC denotes and
T1453 1170-1179 NN denotes microglia
T1452 1166-1169 DT denotes the
T1451 1158-1165 VB denotes deplete
T1450 1155-1157 TO denotes to
T1449 1153-1154 NN denotes h
T1448 1151-1152 CD denotes 4
T1447 1147-1150 IN denotes for
T1446 1143-1146 NN denotes rpm
T1445 1139-1142 CD denotes 150
T1444 1136-1138 IN denotes at
T1443 1129-1135 VB denotes shaken
T1442 1124-1128 VB denotes were
T1441 1117-1123 NN denotes dishes
T1440 1114-1116 CC denotes or
T1439 1107-1113 NN denotes flasks
T1438 1103-1106 DT denotes the
T1437 1098-1102 RB denotes Then
T1436 1086-1096 NN denotes confluence
T1435 1080-1085 VB denotes reach
T1434 1077-1079 TO denotes to
T1433 1072-1076 NN denotes days
T1432 1070-1071 CD denotes 7
T1431 1064-1069 IN denotes about
T1430 1060-1063 IN denotes for
T1429 1051-1059 VB denotes expanded
T1428 1040-1050 NN denotes Astrocytes
T1427 1034-1038 NN denotes week
T1426 1032-1033 DT denotes a
T1425 1026-1031 RB denotes twice
T1424 1013-1025 JJ denotes half-changed
T1423 1009-1012 VB denotes was
T1422 1002-1008 NN denotes medium
T1421 994-1001 NN denotes culture
T1420 985-993 JJ denotes complete
T1419 981-984 DT denotes the
T1418 977-980 CC denotes And
T1417 972-975 NN denotes CO2
T1416 970-971 NN denotes %
T1415 969-970 CD denotes 5
T1414 964-968 IN denotes with
T1413 959-963 NN denotes 37°C
T1412 956-958 IN denotes at
T1411 945-955 VB denotes cultivated
T1410 940-944 VB denotes were
T1409 934-939 NN denotes cells
T1408 930-933 DT denotes the
T1407 925-929 RB denotes Then
T1406 918-923 NN denotes cells
T1405 907-917 JJ denotes epithelial
T1404 898-906 JJ denotes residual
T1403 894-897 DT denotes the
T1402 884-893 VB denotes depleting
T1401 880-883 IN denotes for
T1400 878-879 -RRB- denotes )
T1399 875-878 NNP denotes USA
T1398 873-874 -COMMA- denotes ,
T1397 867-873 NNP denotes Costar
T1396 866-867 -LRB- denotes (
T1395 861-865 JJ denotes dish
T1394 858-860 CC denotes or
T1393 852-857 NN denotes flask
T1392 848-851 JJ denotes new
T1391 846-847 DT denotes a
T1390 843-845 TO denotes to
T1389 831-842 VB denotes transferred
T1388 827-830 VB denotes was
T1387 815-826 NN denotes supernatant
T1386 811-814 DT denotes the
T1385 809-810 -COMMA- denotes ,
T1384 808-809 NN denotes h
T1383 806-807 CD denotes 1
T1382 802-805 IN denotes for
T1381 791-801 NN denotes incubation
T1380 785-790 IN denotes After
T1379 782-783 -RRB- denotes )
T1378 776-782 NN denotes medium
T1377 768-775 NN denotes culture
T1376 759-767 JJ denotes complete
T1375 758-759 -LRB- denotes (
T1374 745-757 NN denotes streptomycin
T1373 739-744 NN denotes μg/mL
T1372 737-738 CD denotes 5
T1371 735-736 -COMMA- denotes ,
T1370 725-735 NN denotes penicillin
T1369 716-724 NN denotes units/mL
T1368 714-715 CD denotes 5
T1367 710-713 CC denotes and
T1366 706-709 NN denotes FBS
T1365 704-705 NN denotes %
T1364 702-704 CD denotes 20
T1363 697-701 IN denotes with
T1362 692-696 NN denotes DMEM
T1361 689-691 IN denotes in
T1360 676-688 NN denotes dissociation
T1359 665-675 JJ denotes mechanical
T1358 663-664 DT denotes a
T1357 660-662 IN denotes by
T1356 651-659 VB denotes followed
T1355 649-650 -COMMA- denotes ,
T1354 646-649 NNP denotes FBS
T1353 644-645 NN denotes %
T1352 642-644 CD denotes 10
T1351 637-641 IN denotes with
T1350 632-636 NN denotes DMEM
T1349 629-631 IN denotes in
T1348 623-628 RB denotes twice
T1347 616-622 VB denotes rinsed
T1346 612-615 VB denotes was
T1345 605-611 NN denotes tissue
T1344 601-604 DT denotes the
T1343 599-600 -COMMA- denotes ,
T1342 590-599 NN denotes digestion
T1341 584-589 IN denotes After
T1340 575-582 NN denotes minutes
T1339 571-574 JJ denotes few
T1338 565-570 DT denotes every
T1337 556-564 VB denotes agitated
T1336 552-555 CC denotes and
T1335 550-551 -COMMA- denotes ,
T1334 546-550 NN denotes 37°C
T1333 543-545 IN denotes at
T1332 535-542 NN denotes minutes
T1331 532-534 CD denotes 10
T1330 528-531 IN denotes for
T1329 520-527 NN denotes trypsin
T1328 518-519 NN denotes %
T1327 515-518 CD denotes 0.5
T1326 512-514 IN denotes in
T1325 502-511 VB denotes incubated
T1324 500-501 -COMMA- denotes ,
T1323 495-500 NN denotes blade
T1322 487-494 NN denotes scalpel
T1321 485-486 DT denotes a
T1320 480-484 IN denotes with
T1916 1980-1985 NN denotes group
T1915 1975-1979 NN denotes sham
T1914 1972-1974 IN denotes as
T1913 1966-1971 VB denotes named
T1912 1962-1965 CC denotes and
T1911 1954-1961 NN denotes control
T1910 1948-1953 JJ denotes blank
T1909 1945-1947 IN denotes as
T1908 1940-1944 VB denotes used
T1907 1935-1939 VB denotes were
T1906 1933-1934 -COMMA- denotes ,
T1905 1926-1933 NN denotes scratch
T1904 1917-1925 VB denotes received
T1903 1913-1916 RB denotes not
T1902 1909-1912 VB denotes did
T1901 1903-1908 WDT denotes which
T1900 1901-1902 -COMMA- denotes ,
T1899 1896-1901 NN denotes Cells
T1898 1889-1894 NN denotes group
T1897 1885-1888 NN denotes TBI
T1896 1882-1884 IN denotes as
T1895 1876-1881 VB denotes named
T1894 1872-1875 CC denotes and
T1893 1870-1871 -COMMA- denotes ,
T1892 1864-1870 NN denotes medium
T1891 1856-1863 NN denotes culture
T1890 1847-1855 JJ denotes complete
T1889 1842-1846 IN denotes with
T1888 1833-1841 VB denotes cultured
T1887 1831-1832 -COMMA- denotes ,
T1886 1828-1831 NNP denotes PBS
T1885 1820-1827 JJ denotes sterile
T1884 1815-1819 IN denotes with
T1883 1809-1814 RB denotes twice
T1882 1802-1808 VB denotes washed
T1881 1797-1801 VB denotes were
T1880 1791-1796 NN denotes cells
T1879 1789-1790 -COMMA- denotes ,
T1877 1776-1781 IN denotes after
T1876 1764-1775 RB denotes Immediately
T1875 1759-1762 NN denotes gap
T1874 1754-1758 JJ denotes wide
T1873 1751-1753 NN denotes mm
T1872 1747-1750 CD denotes 0.5
T1871 1745-1746 DT denotes a
T1870 1742-1744 IN denotes of
T1869 1732-1741 NN denotes formation
T1868 1728-1731 DT denotes the
T1867 1725-1727 IN denotes in
T1866 1715-1724 VB denotes resulting
T1865 1713-1714 -COMMA- denotes ,
T1864 1707-1713 NN denotes needle
T1863 1699-1706 NN denotes syringe
T1862 1695-1698 NN denotes 26G
T1861 1687-1694 JJ denotes sterile
T1860 1685-1686 DT denotes a
T1859 1680-1684 IN denotes with
T1858 1670-1679 VB denotes scratched
T1857 1666-1669 VB denotes was
T1856 1656-1665 NN denotes monolayer
T1855 1651-1655 NN denotes cell
T1854 1647-1650 DT denotes The
T1853 1635-1645 VB denotes confluence
T1852 1632-1634 TO denotes to
T1851 1626-1631 VB denotes grown
T1850 1622-1625 CC denotes and
T1849 1615-1621 NN denotes plates
T1848 1608-1614 JJ denotes 6-well
T1847 1605-1607 IN denotes in
T1846 1597-1604 VB denotes planted
T1845 1592-1596 VB denotes were
T1844 1581-1591 NN denotes Astrocytes
T1843 1578-1579 -RRB- denotes ]
T1842 1577-1578 CD denotes 7
T1841 1576-1577 -LRB- denotes [
T1840 1569-1575 NN denotes report
T1839 1560-1568 JJ denotes previous
T1838 1558-1559 DT denotes a
T1837 1555-1557 IN denotes in
T1836 1552-1554 IN denotes as
T1835 1542-1551 VB denotes performed
T1834 1538-1541 VB denotes was
T1833 1531-1537 NN denotes injury
T1832 1523-1530 NN denotes scratch
T1831 1513-1522 NN denotes Astrocyte
T1830 1506-1512 NNP denotes Injury
T1829 1498-1505 NNP denotes Scratch
T1828 1495-1497 IN denotes by
T1827 1483-1494 VB denotes Established
T1826 1479-1482 NNP denotes TBI
T1825 1476-1478 IN denotes of
T1824 1470-1475 NNP denotes Model
T1823 1464-1469 NNP denotes Vitro
T1822 1461-1463 IN denotes In
T1821 1456-1459 CD denotes 2.2
T1319 472-479 VB denotes chopped
T1318 464-471 RB denotes roughly
T1317 460-463 VB denotes was
T1316 453-459 NN denotes tissue
T1315 449-452 DT denotes The
T1314 440-447 VB denotes removed
T1313 435-439 VB denotes were
T1312 427-434 NN denotes vessels
T1311 421-426 NN denotes blood
T1310 410-420 VB denotes associated
T1309 406-409 CC denotes and
T1308 397-405 NN denotes meninges
T1307 393-396 DT denotes the
T1306 389-392 CC denotes and
T1305 387-388 -COMMA- denotes ,
T1304 384-387 RP denotes out
T1303 374-383 VB denotes dissected
T1302 369-373 VB denotes were
T1301 360-368 NN denotes cortices
T1300 356-359 DT denotes the
T1299 354-355 -COMMA- denotes ,
T1298 342-354 NN denotes decapitation
T1297 332-341 VB denotes Following
T1296 329-330 -RRB- denotes )
T1295 321-329 NN denotes genotype
T1294 316-320 DT denotes each
T1248 86-90 NN denotes Nrf2
T1247 75-85 NN denotes Astrocytes
T1246 69-74 NN denotes Mouse
T1245 66-68 IN denotes of
T1244 51-65 NN denotes Identification
T1243 47-50 CC denotes and
T1242 39-46 NN denotes Culture
T1241 31-38 JJ denotes Primary
T1240 26-29 CD denotes 2.1
T1293 312-315 IN denotes for
T1292 307-311 NN denotes mice
T1291 305-306 CD denotes 6
T1290 304-305 -LRB- denotes (
T1289 299-303 NN denotes mice
T1288 297-298 -RRB- denotes )
T1287 295-297 NN denotes KO
T1286 294-295 -LRB- denotes (
T1285 285-293 NN denotes knockout
T1284 281-284 CC denotes and
T1283 279-280 -RRB- denotes )
T1282 277-279 NN denotes WT
T1281 276-277 -LRB- denotes (
T1280 271-275 NN denotes type
T1279 266-270 JJ denotes wide
T1278 261-265 NN denotes Nrf2
T1277 251-260 JJ denotes 2-day-old
T1276 241-250 JJ denotes postnatal
T1275 236-240 IN denotes from
T1274 227-235 VB denotes obtained
T1273 222-226 VB denotes were
T1272 211-221 NN denotes astrocytes
T1271 203-210 JJ denotes Primary
T1270 200-201 -RRB- denotes )
T1269 197-200 NNP denotes USA
T1268 195-196 -COMMA- denotes ,
T1267 193-195 NNP denotes MD
T1266 191-192 -COMMA- denotes ,
T1265 182-191 NNP denotes Baltimore
T1264 180-181 -COMMA- denotes ,
T1263 170-180 NNP denotes University
T1262 162-169 NNP denotes Hopkins
T1261 156-161 NNP denotes Johns
T1260 155-156 -LRB- denotes (
T1259 147-154 NNP denotes Kensler
T1258 144-146 NNP denotes W.
T1257 137-143 NNP denotes Thomas
T1256 133-136 NNP denotes Dr.
T1255 130-132 IN denotes by
T1254 121-129 VB denotes provided
T1253 114-120 RB denotes kindly
T1252 109-113 VB denotes were
T1251 104-108 NN denotes mice
T1250 100-103 NN denotes ICR
T1249 91-99 NN denotes knockout
T4100 6640-6649 VB denotes collected
T4099 6636-6639 VB denotes was
T4098 6624-6635 NN denotes supernatant
T4097 6620-6623 DT denotes The
T4096 6613-6618 IN denotes above
T4095 6603-6612 VB denotes mentioned
T4094 6600-6602 IN denotes as
T4093 6588-6599 VB denotes homogenized
T4092 6583-6587 VB denotes were
T4091 6576-6582 NN denotes groups
T4090 6571-6575 CD denotes four
T4089 6568-6570 IN denotes of
T4088 6562-6567 NN denotes Cells
T4087 6560-6561 -RRB- denotes )
T4086 6555-6560 NN denotes ELISA
T4085 6554-6555 -LRB- denotes (
T4084 6548-6553 NN denotes Assay
T4083 6534-6547 JJ denotes Immunosorbent
T4082 6520-6533 JJ denotes Enzyme-Linked
T4081 6515-6518 CD denotes 2.7
R985 T1242 T1243 arg1Of Culture,and
R986 T1243 T1241 arg1Of and,Primary
R987 T1243 T1245 arg1Of and,of
R988 T1244 T1243 arg2Of Identification,and
R989 T1247 T1245 arg2Of Astrocytes,of
R990 T1247 T1246 arg1Of Astrocytes,Mouse
R991 T1251 T1248 arg1Of mice,Nrf2
R992 T1251 T1249 arg1Of mice,knockout
R993 T1251 T1250 arg1Of mice,ICR
R994 T1251 T1252 arg1Of mice,were
R995 T1251 T1254 arg2Of mice,provided
R996 T1254 T1252 arg2Of provided,were
R997 T1254 T1253 arg1Of provided,kindly
R998 T1259 T1254 arg1Of Kensler,provided
R999 T1259 T1255 arg2Of Kensler,by
R1000 T1259 T1256 arg1Of Kensler,Dr.
R1001 T1259 T1257 arg1Of Kensler,Thomas
R1002 T1259 T1258 arg1Of Kensler,W.
R1003 T1259 T1260 arg1Of Kensler,(
R1004 T1263 T1260 arg2Of University,(
R1005 T1263 T1261 arg1Of University,Johns
R1006 T1263 T1262 arg1Of University,Hopkins
R1007 T1263 T1264 arg1Of University,","
R1008 T1263 T1268 arg1Of University,","
R1009 T1263 T1269 arg1Of University,USA
R1010 T1265 T1264 arg2Of Baltimore,","
R1011 T1265 T1266 arg1Of Baltimore,","
R1012 T1267 T1266 arg2Of MD,","
R1013 T1270 T1260 arg3Of ),(
R1014 T1272 T1271 arg1Of astrocytes,Primary
R1015 T1272 T1273 arg1Of astrocytes,were
R1016 T1272 T1274 arg2Of astrocytes,obtained
R1017 T1274 T1273 arg2Of obtained,were
R1018 T1274 T1275 arg1Of obtained,from
R1019 T1280 T1276 arg1Of type,postnatal
R1020 T1280 T1277 arg1Of type,2-day-old
R1021 T1280 T1278 arg1Of type,Nrf2
R1022 T1280 T1279 arg1Of type,wide
R1023 T1280 T1281 arg1Of type,(
R1024 T1280 T1284 arg1Of type,and
R1025 T1282 T1281 arg2Of WT,(
R1026 T1283 T1281 arg3Of ),(
R1027 T1284 T1275 arg2Of and,from
R1028 T1287 T1286 arg2Of KO,(
R1029 T1288 T1286 arg3Of ),(
R1030 T1289 T1284 arg2Of mice,and
R1031 T1289 T1285 arg1Of mice,knockout
R1032 T1289 T1286 arg1Of mice,(
R1033 T1289 T1290 arg1Of mice,(
R1034 T1292 T1290 arg2Of mice,(
R1035 T1292 T1291 arg1Of mice,6
R1036 T1292 T1293 arg1Of mice,for
R1037 T1295 T1293 arg2Of genotype,for
R1038 T1295 T1294 arg1Of genotype,each
R1039 T1296 T1290 arg3Of ),(
R1040 T1298 T1297 arg2Of decapitation,Following
R1041 T1301 T1300 arg1Of cortices,the
R1042 T1301 T1302 arg1Of cortices,were
R1043 T1301 T1303 arg2Of cortices,dissected
R1044 T1303 T1302 arg2Of dissected,were
R1045 T1303 T1304 arg1Of dissected,out
R1046 T1303 T1306 arg1Of dissected,and
R1047 T1306 T1297 arg1Of and,Following
R1048 T1306 T1299 arg1Of and,","
R1049 T1306 T1305 arg1Of and,","
R1050 T1308 T1309 arg1Of meninges,and
R1051 T1309 T1307 arg1Of and,the
R1052 T1309 T1313 arg1Of and,were
R1053 T1309 T1314 arg2Of and,removed
R1054 T1312 T1309 arg2Of vessels,and
R1055 T1312 T1310 arg2Of vessels,associated
R1056 T1312 T1311 arg1Of vessels,blood
R1057 T1314 T1306 arg2Of removed,and
R1058 T1314 T1313 arg2Of removed,were
R1059 T1316 T1315 arg1Of tissue,The
R1060 T1316 T1317 arg1Of tissue,was
R1061 T1316 T1319 arg2Of tissue,chopped
R1062 T1316 T1337 arg1Of tissue,agitated
R1063 T1319 T1317 arg2Of chopped,was
R1064 T1319 T1318 arg1Of chopped,roughly
R1065 T1319 T1320 arg1Of chopped,with
R1066 T1319 T1336 arg1Of chopped,and
R1067 T1323 T1320 arg2Of blade,with
R1068 T1323 T1321 arg1Of blade,a
R1069 T1323 T1322 arg1Of blade,scalpel
R1070 T1323 T1324 arg1Of blade,","
R1071 T1323 T1325 arg2Of blade,incubated
R1072 T1325 T1326 arg1Of incubated,in
R1073 T1327 T1328 arg1Of 0.5,%
R1074 T1329 T1326 arg2Of trypsin,in
R1075 T1329 T1327 arg1Of trypsin,0.5
R1076 T1329 T1330 arg1Of trypsin,for
R1077 T1329 T1333 arg1Of trypsin,at
R1078 T1332 T1330 arg2Of minutes,for
R1079 T1332 T1331 arg1Of minutes,10
R1080 T1334 T1333 arg2Of 37°C,at
R1081 T1336 T1335 arg1Of and,","
R1082 T1337 T1336 arg2Of agitated,and
R1083 T1340 T1337 arg2Of minutes,agitated
R1084 T1340 T1338 arg1Of minutes,every
R1085 T1340 T1339 arg1Of minutes,few
R1086 T1342 T1341 arg2Of digestion,After
R1087 T1345 T1344 arg1Of tissue,the
R1088 T1345 T1346 arg1Of tissue,was
R1089 T1345 T1347 arg2Of tissue,rinsed
R1090 T1347 T1341 arg1Of rinsed,After
R1091 T1347 T1343 arg1Of rinsed,","
R1092 T1347 T1346 arg2Of rinsed,was
R1093 T1347 T1348 arg1Of rinsed,twice
R1094 T1347 T1349 arg1Of rinsed,in
R1095 T1350 T1349 arg2Of DMEM,in
R1096 T1350 T1351 arg1Of DMEM,with
R1097 T1352 T1353 arg1Of 10,%
R1098 T1354 T1352 arg1Of FBS,10
R1099 T1354 T1355 arg1Of FBS,","
R1100 T1354 T1356 arg2Of FBS,followed
R1101 T1354 T1371 arg1Of FBS,","
R1102 T1360 T1356 arg1Of dissociation,followed
R1103 T1360 T1357 arg2Of dissociation,by
R1104 T1360 T1358 arg1Of dissociation,a
R1105 T1360 T1359 arg1Of dissociation,mechanical
R1106 T1360 T1361 arg1Of dissociation,in
R1107 T1362 T1361 arg2Of DMEM,in
R1108 T1362 T1363 arg1Of DMEM,with
R1109 T1366 T1364 arg1Of FBS,20
R1110 T1366 T1365 arg1Of FBS,%
R1111 T1366 T1367 arg1Of FBS,and
R1112 T1367 T1363 arg2Of and,with
R1113 T1370 T1367 arg2Of penicillin,and
R1114 T1370 T1368 arg1Of penicillin,5
R1115 T1370 T1369 arg1Of penicillin,units/mL
R1116 T1371 T1351 arg2Of ",",with
R1117 T1374 T1371 arg2Of streptomycin,","
R1118 T1374 T1372 arg1Of streptomycin,5
R1119 T1374 T1373 arg1Of streptomycin,μg/mL
R1120 T1374 T1375 arg1Of streptomycin,(
R1121 T1378 T1375 arg2Of medium,(
R1122 T1378 T1376 arg1Of medium,complete
R1123 T1378 T1377 arg1Of medium,culture
R1124 T1379 T1375 arg3Of ),(
R1125 T1381 T1380 arg2Of incubation,After
R1126 T1381 T1382 arg1Of incubation,for
R1127 T1384 T1382 arg2Of h,for
R1128 T1384 T1383 arg1Of h,1
R1129 T1387 T1386 arg1Of supernatant,the
R1130 T1387 T1388 arg1Of supernatant,was
R1131 T1387 T1389 arg2Of supernatant,transferred
R1132 T1389 T1380 arg1Of transferred,After
R1133 T1389 T1385 arg1Of transferred,","
R1134 T1389 T1388 arg2Of transferred,was
R1135 T1389 T1390 arg1Of transferred,to
R1136 T1389 T1396 arg1Of transferred,(
R1137 T1389 T1401 arg1Of transferred,for
R1138 T1393 T1394 arg1Of flask,or
R1139 T1394 T1390 arg2Of or,to
R1140 T1394 T1391 arg1Of or,a
R1141 T1394 T1392 arg1Of or,new
R1142 T1395 T1394 arg2Of dish,or
R1143 T1397 T1396 arg2Of Costar,(
R1144 T1397 T1398 arg1Of Costar,","
R1145 T1397 T1399 arg1Of Costar,USA
R1146 T1400 T1396 arg3Of ),(
R1147 T1402 T1401 arg2Of depleting,for
R1148 T1406 T1402 arg2Of cells,depleting
R1149 T1406 T1403 arg1Of cells,the
R1150 T1406 T1404 arg1Of cells,residual
R1151 T1406 T1405 arg1Of cells,epithelial
R1152 T1409 T1408 arg1Of cells,the
R1153 T1409 T1410 arg1Of cells,were
R1154 T1409 T1411 arg2Of cells,cultivated
R1155 T1411 T1407 arg1Of cultivated,Then
R1156 T1411 T1410 arg2Of cultivated,were
R1157 T1411 T1412 arg1Of cultivated,at
R1158 T1413 T1412 arg2Of 37°C,at
R1159 T1413 T1414 arg1Of 37°C,with
R1160 T1415 T1416 arg1Of 5,%
R1161 T1417 T1414 arg2Of CO2,with
R1162 T1417 T1415 arg1Of CO2,5
R1163 T1422 T1419 arg1Of medium,the
R1164 T1422 T1420 arg1Of medium,complete
R1165 T1422 T1421 arg1Of medium,culture
R1166 T1422 T1423 arg1Of medium,was
R1167 T1422 T1424 arg1Of medium,half-changed
R1168 T1423 T1418 arg1Of was,And
R1169 T1423 T1425 arg1Of was,twice
R1170 T1423 T1427 arg1Of was,week
R1171 T1424 T1423 arg2Of half-changed,was
R1172 T1427 T1426 arg1Of week,a
R1173 T1428 T1429 arg2Of Astrocytes,expanded
R1174 T1428 T1435 arg1Of Astrocytes,reach
R1175 T1429 T1430 arg1Of expanded,for
R1176 T1429 T1434 modOf expanded,to
R1177 T1432 T1431 arg1Of 7,about
R1178 T1433 T1430 arg2Of days,for
R1179 T1433 T1432 arg1Of days,7
R1180 T1435 T1434 arg1Of reach,to
R1181 T1436 T1435 arg2Of confluence,reach
R1182 T1439 T1440 arg1Of flasks,or
R1183 T1440 T1438 arg1Of or,the
R1184 T1440 T1442 arg1Of or,were
R1185 T1440 T1443 arg2Of or,shaken
R1186 T1441 T1440 arg2Of dishes,or
R1187 T1443 T1437 arg1Of shaken,Then
R1188 T1443 T1442 arg2Of shaken,were
R1189 T1443 T1444 arg1Of shaken,at
R1190 T1446 T1444 arg2Of rpm,at
R1191 T1446 T1445 arg1Of rpm,150
R1192 T1446 T1447 arg1Of rpm,for
R1193 T1449 T1447 arg2Of h,for
R1194 T1449 T1448 arg1Of h,4
R1195 T1449 T1451 arg1Of h,deplete
R1196 T1451 T1447 arg3Of deplete,for
R1197 T1451 T1450 arg1Of deplete,to
R1198 T1451 T1458 arg1Of deplete,from
R1199 T1453 T1454 arg1Of microglia,and
R1200 T1454 T1451 arg2Of and,deplete
R1201 T1454 T1452 arg1Of and,the
R1202 T1456 T1455 arg1Of adherent,less
R1203 T1457 T1454 arg2Of cells,and
R1204 T1457 T1456 arg1Of cells,adherent
R1205 T1460 T1458 arg2Of cultures,from
R1206 T1460 T1459 arg1Of cultures,the
R1207 T1462 T1461 arg2Of shaking,After
R1208 T1466 T1462 arg1Of cultures,shaking
R1209 T1466 T1464 arg1Of cultures,the
R1210 T1466 T1465 arg1Of cultures,resulting
R1211 T1466 T1467 arg1Of cultures,were
R1212 T1467 T1461 arg1Of were,After
R1213 T1467 T1463 arg1Of were,","
R1214 T1469 T1467 arg2Of astrocytes,were
R1215 T1469 T1468 arg1Of astrocytes,mainly
R1216 T1469 T1470 arg1Of astrocytes,","
R1217 T1469 T1471 arg1Of astrocytes,which
R1218 T1469 T1472 arg1Of astrocytes,were
R1219 T1469 T1473 arg2Of astrocytes,determined
R1220 T1473 T1472 arg2Of determined,were
R1221 T1473 T1474 arg1Of determined,by
R1222 T1475 T1474 arg2Of immunoreactivity,by
R1223 T1475 T1476 arg1Of immunoreactivity,for
R1224 T1477 T1476 arg2Of GFAP,for
R1225 T1477 T1478 arg1Of GFAP,(
R1226 T1479 T1480 arg1Of sc-166481,","
R1227 T1479 T1484 arg1Of sc-166481,","
R1228 T1483 T1480 arg2Of Biotechnology,","
R1229 T1483 T1481 arg1Of Biotechnology,Santa
R1230 T1483 T1482 arg1Of Biotechnology,Cruz
R1231 T1484 T1478 arg2Of ",",(
R1232 T1485 T1484 arg2Of CA,","
R1233 T1485 T1486 arg1Of CA,","
R1234 T1487 T1486 arg2Of USA,","
R1235 T1488 T1478 arg3Of ),(
R1236 T1489 T1490 arg2Of Cells,passaged
R1237 T1489 T1494 arg1Of Cells,were
R1238 T1489 T1495 arg2Of Cells,used
R1239 T1490 T1491 arg1Of passaged,for
R1240 T1493 T1491 arg2Of generations,for
R1241 T1493 T1492 arg1Of generations,2-3
R1242 T1495 T1494 arg2Of used,were
R1243 T1495 T1496 arg1Of used,in
R1244 T1499 T1496 arg2Of studies,in
R1245 T1499 T1497 arg1Of studies,the
R1246 T1499 T1498 arg1Of studies,following
R1526 T1824 T1822 arg2Of Model,In
R1527 T1824 T1823 arg1Of Model,Vitro
R1528 T1824 T1825 arg1Of Model,of
R1529 T1824 T1827 arg2Of Model,Established
R1530 T1826 T1825 arg2Of TBI,of
R1531 T1830 T1827 arg1Of Injury,Established
R1532 T1830 T1828 arg2Of Injury,by
R1533 T1830 T1829 arg1Of Injury,Scratch
R1534 T1833 T1831 arg1Of injury,Astrocyte
R1535 T1833 T1832 arg1Of injury,scratch
R1536 T1833 T1834 arg1Of injury,was
R1537 T1833 T1835 arg2Of injury,performed
R1538 T1835 T1834 arg2Of performed,was
R1539 T1835 T1837 arg1Of performed,in
R1540 T1835 T1841 arg1Of performed,[
R1541 T1837 T1836 arg1Of in,as
R1542 T1840 T1837 arg2Of report,in
R1543 T1840 T1838 arg1Of report,a
R1544 T1840 T1839 arg1Of report,previous
R1545 T1842 T1841 arg2Of 7,[
R1546 T1843 T1841 arg3Of ],[
R1547 T1844 T1845 arg1Of Astrocytes,were
R1548 T1844 T1846 arg2Of Astrocytes,planted
R1549 T1844 T1851 arg2Of Astrocytes,grown
R1550 T1846 T1847 arg1Of planted,in
R1551 T1846 T1850 arg1Of planted,and
R1552 T1849 T1847 arg2Of plates,in
R1553 T1849 T1848 arg1Of plates,6-well
R1554 T1850 T1845 arg2Of and,were
R1555 T1850 T1852 modOf and,to
R1556 T1851 T1850 arg2Of grown,and
R1557 T1853 T1852 arg1Of confluence,to
R1558 T1856 T1854 arg1Of monolayer,The
R1559 T1856 T1855 arg1Of monolayer,cell
R1560 T1856 T1857 arg1Of monolayer,was
R1561 T1856 T1858 arg2Of monolayer,scratched
R1562 T1858 T1857 arg2Of scratched,was
R1563 T1858 T1859 arg1Of scratched,with
R1564 T1858 T1865 arg1Of scratched,","
R1565 T1858 T1866 modOf scratched,resulting
R1566 T1864 T1859 arg2Of needle,with
R1567 T1864 T1860 arg1Of needle,a
R1568 T1864 T1861 arg1Of needle,sterile
R1569 T1864 T1862 arg1Of needle,26G
R1570 T1864 T1863 arg1Of needle,syringe
R1571 T1866 T1867 arg1Of resulting,in
R1572 T1869 T1867 arg2Of formation,in
R1573 T1869 T1868 arg1Of formation,the
R1574 T1869 T1870 arg1Of formation,of
R1575 T1875 T1870 arg2Of gap,of
R1576 T1875 T1871 arg1Of gap,a
R1577 T1875 T1872 arg1Of gap,0.5
R1578 T1875 T1873 arg1Of gap,mm
R1579 T1875 T1874 arg1Of gap,wide
R1580 T1877 T1876 arg1Of after,Immediately
R1581 T1878 T1877 arg2Of scratch,after
R1582 T1880 T1881 arg1Of cells,were
R1583 T1880 T1882 arg2Of cells,washed
R1584 T1880 T1895 arg2Of cells,named
R1585 T1882 T1883 arg1Of washed,twice
R1586 T1882 T1884 arg1Of washed,with
R1587 T1882 T1894 arg1Of washed,and
R1588 T1886 T1884 arg2Of PBS,with
R1589 T1886 T1885 arg1Of PBS,sterile
R1590 T1886 T1887 arg1Of PBS,","
R1591 T1886 T1888 arg2Of PBS,cultured
R1592 T1888 T1889 arg1Of cultured,with
R1593 T1892 T1889 arg2Of medium,with
R1594 T1892 T1890 arg1Of medium,complete
R1595 T1892 T1891 arg1Of medium,culture
R1596 T1894 T1877 arg1Of and,after
R1597 T1894 T1879 arg1Of and,","
R1598 T1894 T1881 arg2Of and,were
R1599 T1894 T1893 arg1Of and,","
R1600 T1895 T1894 arg2Of named,and
R1601 T1895 T1896 arg1Of named,as
R1602 T1898 T1896 arg2Of group,as
R1603 T1898 T1897 arg1Of group,TBI
R1604 T1899 T1900 arg1Of Cells,","
R1605 T1899 T1901 arg1Of Cells,which
R1606 T1899 T1902 arg1Of Cells,did
R1607 T1899 T1907 arg1Of Cells,were
R1608 T1899 T1908 arg2Of Cells,used
R1609 T1899 T1913 arg2Of Cells,named
R1610 T1902 T1903 arg1Of did,not
R1611 T1905 T1902 arg2Of scratch,did
R1612 T1905 T1904 arg2Of scratch,received
R1613 T1908 T1909 arg1Of used,as
R1614 T1908 T1912 arg1Of used,and
R1615 T1911 T1909 arg2Of control,as
R1616 T1911 T1910 arg1Of control,blank
R1617 T1912 T1906 arg1Of and,","
R1618 T1912 T1907 arg2Of and,were
R1619 T1913 T1912 arg2Of named,and
R1620 T1913 T1914 arg1Of named,as
R1621 T1916 T1914 arg2Of group,as
R1622 T1916 T1915 arg1Of group,sham
R1726 T2050 T2048 arg1Of Measurement,Cell
R1727 T2050 T2049 arg1Of Measurement,Death
R1728 T2050 T2051 arg1Of Measurement,by
R1729 T2053 T2051 arg2Of Assay,by
R1730 T2053 T2052 arg1Of Assay,CCK-8
R1731 T2057 T2054 arg1Of cells,1
R1732 T2057 T2055 arg1Of cells,×
R1733 T2057 T2056 arg1Of cells,103/well
R1734 T2057 T2058 arg1Of cells,were
R1735 T2057 T2059 arg2Of cells,seeded
R1736 T2057 T2065 arg2Of cells,cultivated
R1737 T2059 T2060 arg1Of seeded,to
R1738 T2059 T2064 arg1Of seeded,and
R1739 T2063 T2060 arg2Of plates,to
R1740 T2063 T2061 arg1Of plates,96-well
R1741 T2063 T2062 arg1Of plates,culture
R1742 T2064 T2058 arg2Of and,were
R1743 T2065 T2064 arg2Of cultivated,and
R1744 T2065 T2066 arg1Of cultivated,for
R1745 T2068 T2066 arg2Of h,for
R1746 T2068 T2067 arg1Of h,24
R1747 T2068 T2070 arg1Of h,adhere
R1748 T2070 T2066 arg3Of adhere,for
R1749 T2070 T2069 arg1Of adhere,to
R1750 T2073 T2072 arg1Of cell,the
R1751 T2073 T2074 arg1Of cell,was
R1752 T2073 T2075 arg2Of cell,scrated
R1753 T2075 T2071 arg1Of scrated,Then
R1754 T2075 T2074 arg2Of scrated,was
R1755 T2075 T2076 arg1Of scrated,as
R1756 T2077 T2076 arg2Of mentioned,as
R1757 T2077 T2078 arg1Of mentioned,above
R1758 T2081 T2079 arg1Of death,Astrocyte
R1759 T2081 T2080 arg1Of death,cell
R1760 T2081 T2082 arg1Of death,was
R1761 T2081 T2083 arg2Of death,assessed
R1762 T2083 T2082 arg2Of assessed,was
R1763 T2083 T2091 arg1Of assessed,(
R1764 T2083 T2101 arg1Of assessed,according
R1765 T2087 T2083 arg1Of kit-8,assessed
R1766 T2087 T2084 arg2Of kit-8,by
R1767 T2087 T2085 arg1Of kit-8,cell
R1768 T2087 T2086 arg1Of kit-8,counting
R1769 T2087 T2088 arg1Of kit-8,(
R1770 T2089 T2088 arg2Of CCK-8,(
R1771 T2090 T2088 arg3Of ),(
R1772 T2092 T2091 arg2Of Dojindo,(
R1773 T2092 T2093 arg1Of Dojindo,","
R1774 T2094 T2093 arg2Of Japan,","
R1775 T2095 T2091 arg3Of ),(
R1776 T2098 T2096 arg1Of h,assay
R1777 T2098 T2097 arg1Of h,24
R1778 T2098 T2099 arg1Of h,after
R1779 T2100 T2099 arg2Of scratch,after
R1780 T2101 T2098 arg1Of according,h
R1781 T2102 T2101 arg2Of to,according
R1782 T2104 T2103 arg1Of manufacturer,the
R1783 T2104 T2105 arg2Of manufacturer,'s
R1784 T2106 T2102 arg2Of protocol,to
R1785 T2106 T2105 arg1Of protocol,'s
R1786 T2107 T2108 arg1Of Briefly,","
R1787 T2107 T2112 arg1Of Briefly,was
R1788 T2107 T2113 arg2Of Briefly,added
R1789 T2107 T2118 arg2Of Briefly,incubated
R1790 T2111 T2108 arg2Of CCK-8,","
R1791 T2111 T2109 arg1Of CCK-8,10
R1792 T2111 T2110 arg1Of CCK-8,μL
R1793 T2113 T2114 arg1Of added,into
R1794 T2113 T2117 arg1Of added,and
R1795 T2116 T2114 arg2Of well,into
R1796 T2116 T2115 arg1Of well,every
R1797 T2117 T2112 arg2Of and,was
R1798 T2118 T2117 arg2Of incubated,and
R1799 T2118 T2119 arg1Of incubated,for
R1800 T2121 T2119 arg2Of h,for
R1801 T2121 T2120 arg1Of h,1
R1802 T2124 T2123 arg1Of value,OD
R1803 T2124 T2125 arg1Of value,was
R1804 T2124 T2126 arg2Of value,read
R1805 T2124 T2130 arg1Of value,using
R1806 T2126 T2122 arg1Of read,Then
R1807 T2126 T2125 arg2Of read,was
R1808 T2126 T2127 arg1Of read,at
R1809 T2126 T2130 modOf read,using
R1810 T2129 T2127 arg2Of nm,at
R1811 T2129 T2128 arg1Of nm,450
R1812 T2130 T2136 arg1Of using,(
R1813 T2135 T2130 arg2Of reader,using
R1814 T2135 T2131 arg1Of reader,a
R1815 T2135 T2132 arg1Of reader,Bio-Rad
R1816 T2135 T2133 arg1Of reader,ELISA
R1817 T2135 T2134 arg1Of reader,microplate
R1818 T2138 T2136 arg2Of Laboratories,(
R1819 T2138 T2137 arg1Of Laboratories,Bio-Rad
R1820 T2138 T2139 arg1Of Laboratories,","
R1821 T2140 T2139 arg2Of CA,","
R1822 T2140 T2141 arg1Of CA,","
R1823 T2142 T2141 arg2Of USA,","
R1824 T2143 T2136 arg3Of ),(
R1825 T2145 T2144 arg1Of measurements,All
R1826 T2145 T2146 arg1Of measurements,were
R1827 T2145 T2147 arg2Of measurements,performed
R1828 T2147 T2146 arg2Of performed,were
R1829 T2147 T2148 arg1Of performed,in
R1830 T2149 T2148 arg2Of sextuplicate,in
R1831 T2150 T2151 arg1Of Results,were
R1832 T2150 T2152 arg2Of Results,expressed
R1833 T2152 T2151 arg2Of expressed,were
R1834 T2152 T2153 arg1Of expressed,as
R1835 T2152 T2158 arg1Of expressed,at
R1836 T2154 T2153 arg2Of mean,as
R1837 T2154 T2155 arg1Of mean,of
R1838 T2157 T2155 arg2Of value,of
R1839 T2157 T2156 arg1Of value,OD
R1840 T2159 T2160 arg1Of 450,nm
R1841 T2162 T2158 arg2Of SD,at
R1842 T2162 T2159 arg1Of SD,450
R1843 T2162 T2161 arg1Of SD,±
R1968 T2327 T2324 arg1Of Assay,Electrophoretic
R1969 T2327 T2325 arg1Of Assay,Mobility
R1970 T2327 T2326 arg1Of Assay,Shift
R1971 T2327 T2328 arg1Of Assay,(
R1972 T2329 T2328 arg2Of EMSA,(
R1973 T2330 T2328 arg3Of ),(
R1974 T2331 T2332 arg1Of Monolayers,of
R1975 T2331 T2334 arg1Of Monolayers,were
R1976 T2331 T2335 arg2Of Monolayers,washed
R1977 T2331 T2339 arg2Of Monolayers,harvested
R1978 T2333 T2332 arg2Of astrocytes,of
R1979 T2335 T2336 arg1Of washed,with
R1980 T2335 T2338 arg1Of washed,and
R1981 T2337 T2336 arg2Of PBS,with
R1982 T2338 T2334 arg2Of and,were
R1983 T2339 T2338 arg2Of harvested,and
R1984 T2339 T2340 arg1Of harvested,by
R1985 T2341 T2340 arg2Of scraping,by
R1986 T2341 T2342 arg1Of scraping,into
R1987 T2344 T2342 arg2Of PBS,into
R1988 T2344 T2343 arg1Of PBS,cold
R1989 T2347 T2345 arg1Of pellet,The
R1990 T2347 T2346 arg1Of pellet,cell
R1991 T2347 T2348 arg2Of pellet,obtained
R1992 T2347 T2351 arg1Of pellet,was
R1993 T2347 T2352 arg2Of pellet,resuspended
R1994 T2350 T2348 arg1Of centrifugation,obtained
R1995 T2350 T2349 arg2Of centrifugation,by
R1996 T2352 T2351 arg2Of resuspended,was
R1997 T2352 T2353 arg1Of resuspended,in
R1998 T2354 T2355 arg1Of buffer,containing
R1999 T2354 T2367 arg1Of buffer,","
R2000 T2356 T2357 arg1Of 10,mM
R2001 T2358 T2356 arg1Of HEPES,10
R2002 T2358 T2359 arg1Of HEPES,(
R2003 T2358 T2363 arg1Of HEPES,","
R2004 T2360 T2359 arg2Of pH,(
R2005 T2360 T2361 arg1Of pH,7.9
R2006 T2362 T2359 arg3Of ),(
R2007 T2363 T2355 arg2Of ",",containing
R2008 T2364 T2365 arg1Of 10,mM
R2009 T2366 T2363 arg2Of KCl,","
R2010 T2366 T2364 arg1Of KCl,10
R2011 T2367 T2353 arg2Of ",",in
R2012 T2368 T2369 arg1Of 0.1,mM
R2013 T2370 T2368 arg1Of EDTA,0.1
R2014 T2370 T2371 arg1Of EDTA,","
R2015 T2371 T2375 arg1Of ",",","
R2016 T2372 T2373 arg1Of 0.1,mM
R2017 T2374 T2371 arg2Of EGTA,","
R2018 T2374 T2372 arg1Of EGTA,0.1
R2019 T2375 T2380 arg1Of ",",and
R2020 T2376 T2377 arg1Of 1.0,mM
R2021 T2378 T2375 arg2Of DTT,","
R2022 T2378 T2376 arg1Of DTT,1.0
R2023 T2380 T2367 arg2Of and,","
R2024 T2380 T2379 arg1Of and,","
R2025 T2381 T2382 arg1Of 0.5,mM
R2026 T2384 T2380 arg2Of fluoride,and
R2027 T2384 T2381 arg1Of fluoride,0.5
R2028 T2384 T2383 arg1Of fluoride,phenylmethylsulfonyl
R2029 T2386 T2385 arg1Of 10,Then
R2030 T2386 T2387 arg1Of 10,%
R2031 T2389 T2386 arg1Of P-40,10
R2032 T2389 T2388 arg1Of P-40,Nonidet
R2033 T2389 T2390 arg1Of P-40,was
R2034 T2389 T2391 arg2Of P-40,added
R2035 T2389 T2393 arg2Of P-40,vortexed
R2036 T2391 T2392 arg1Of added,and
R2037 T2392 T2390 arg2Of and,was
R2038 T2392 T2396 arg1Of and,and
R2039 T2393 T2392 arg2Of vortexed,and
R2040 T2394 T2393 arg3Of briefly,vortexed
R2041 T2396 T2395 arg1Of and,","
R2042 T2398 T2397 arg1Of nuclei,the
R2043 T2398 T2399 arg1Of nuclei,were
R2044 T2398 T2400 arg2Of nuclei,pelleted
R2045 T2400 T2396 arg2Of pelleted,and
R2046 T2400 T2399 arg2Of pelleted,were
R2047 T2402 T2400 arg1Of centrifugation,pelleted
R2048 T2402 T2401 arg2Of centrifugation,by
R2049 T2405 T2403 arg1Of proteins,The
R2050 T2405 T2404 arg1Of proteins,nuclear
R2051 T2405 T2406 arg1Of proteins,were
R2052 T2405 T2407 arg2Of proteins,extracted
R2053 T2407 T2406 arg2Of extracted,were
R2054 T2407 T2408 arg1Of extracted,with
R2055 T2409 T2410 arg1Of buffer,containing
R2056 T2409 T2422 arg1Of buffer,","
R2057 T2411 T2412 arg1Of 20,mM
R2058 T2413 T2411 arg1Of HEPES,20
R2059 T2413 T2414 arg1Of HEPES,(
R2060 T2413 T2418 arg1Of HEPES,","
R2061 T2415 T2414 arg2Of pH,(
R2062 T2415 T2416 arg1Of pH,7.9
R2063 T2417 T2414 arg3Of ),(
R2064 T2418 T2410 arg2Of ",",containing
R2065 T2419 T2420 arg1Of 0.4,mM
R2066 T2421 T2418 arg2Of NaCl,","
R2067 T2421 T2419 arg1Of NaCl,0.4
R2068 T2422 T2408 arg2Of ",",with
R2069 T2423 T2424 arg1Of 1.0,mM
R2070 T2425 T2423 arg1Of EDTA,1.0
R2071 T2425 T2426 arg1Of EDTA,","
R2072 T2426 T2430 arg1Of ",",","
R2073 T2427 T2428 arg1Of 1.0,mM
R2074 T2429 T2426 arg2Of EGTA,","
R2075 T2429 T2427 arg1Of EGTA,1.0
R2076 T2430 T2435 arg1Of ",",and
R2077 T2431 T2432 arg1Of 1.0,mM
R2078 T2433 T2430 arg2Of DTT,","
R2079 T2433 T2431 arg1Of DTT,1.0
R2080 T2435 T2422 arg2Of and,","
R2081 T2435 T2434 arg1Of and,","
R2082 T2436 T2437 arg1Of 1.0,mM
R2083 T2439 T2435 arg2Of fluoride,and
R2084 T2439 T2436 arg1Of fluoride,1.0
R2085 T2439 T2438 arg1Of fluoride,phenylmethylsulfonyl
R2086 T2441 T2440 arg1Of material,Insoluble
R2087 T2441 T2442 arg1Of material,was
R2088 T2441 T2443 arg2Of material,removed
R2089 T2443 T2442 arg2Of removed,was
R2090 T2443 T2446 arg1Of removed,at
R2091 T2443 T2450 arg1Of removed,and
R2092 T2445 T2443 arg1Of centrifugation,removed
R2093 T2445 T2444 arg2Of centrifugation,by
R2094 T2448 T2446 arg2Of rpm,at
R2095 T2448 T2447 arg1Of rpm,14000
R2096 T2450 T2449 arg1Of and,","
R2097 T2452 T2451 arg1Of supernatant,the
R2098 T2452 T2453 arg1Of supernatant,containing
R2099 T2452 T2457 arg1Of supernatant,was
R2100 T2452 T2458 arg2Of supernatant,stored
R2101 T2456 T2453 arg2Of proteins,containing
R2102 T2456 T2454 arg1Of proteins,the
R2103 T2456 T2455 arg1Of proteins,nuclear
R2104 T2458 T2450 arg2Of stored,and
R2105 T2458 T2457 arg2Of stored,was
R2106 T2458 T2459 arg1Of stored,at
R2107 T2458 T2461 arg1Of stored,until
R2108 T2460 T2459 arg2Of –80°C,at
R2109 T2462 T2461 arg2Of use,until
R2110 T2464 T2463 arg1Of concentration,Protein
R2111 T2464 T2465 arg1Of concentration,was
R2112 T2464 T2466 arg2Of concentration,determined
R2113 T2466 T2465 arg2Of determined,was
R2114 T2467 T2466 arg3Of using,determined
R2115 T2467 T2473 arg1Of using,with
R2116 T2472 T2467 arg2Of kit,using
R2117 T2472 T2468 arg1Of kit,a
R2118 T2472 T2469 arg1Of kit,bicinchoninic
R2119 T2472 T2470 arg1Of kit,acid
R2120 T2472 T2471 arg1Of kit,assay
R2121 T2476 T2473 arg2Of albumin,with
R2122 T2476 T2474 arg1Of albumin,bovine
R2123 T2476 T2475 arg1Of albumin,serum
R2124 T2476 T2477 arg1Of albumin,as
R2125 T2482 T2481 arg1Of Biochemicals,Pierce
R2126 T2482 T2483 arg1Of Biochemicals,","
R2127 T2483 T2485 arg1Of ",",","
R2128 T2484 T2483 arg2Of Rockford,","
R2129 T2485 T2477 arg2Of ",",as
R2130 T2485 T2478 arg1Of ",",the
R2131 T2485 T2479 arg1Of ",",standard
R2132 T2485 T2480 arg2Of ",",(
R2133 T2485 T2487 arg1Of ",",","
R2134 T2485 T2488 arg1Of ",",USA
R2135 T2486 T2485 arg2Of IL,","
R2136 T2489 T2480 arg3Of ),(
R2137 T2490 T2491 arg1Of EMSA,was
R2138 T2490 T2492 arg2Of EMSA,performed
R2139 T2492 T2491 arg2Of performed,was
R2140 T2493 T2492 arg3Of using,performed
R2141 T2497 T2493 arg2Of system,using
R2142 T2497 T2494 arg1Of system,gel
R2143 T2497 T2495 arg1Of system,shift
R2144 T2497 T2496 arg1Of system,assay
R2145 T2497 T2498 arg1Of system,(
R2146 T2499 T2500 arg1Of Promega,","
R2147 T2500 T2502 arg1Of ",",","
R2148 T2501 T2500 arg2Of Madison,","
R2149 T2502 T2498 arg2Of ",",(
R2150 T2502 T2504 arg1Of ",",","
R2151 T2502 T2505 arg1Of ",",USA
R2152 T2503 T2502 arg2Of WI,","
R2153 T2506 T2498 arg3Of ),(
R2154 T2509 T2507 arg1Of probe,Consensus
R2155 T2509 T2508 arg1Of probe,oligonucleotide
R2156 T2509 T2510 arg1Of probe,(
R2157 T2509 T2514 arg1Of probe,was
R2158 T2509 T2515 arg2Of probe,end-labeled
R2159 T2509 T2519 arg1Of probe,using
R2160 T2511 T2510 arg2Of 5′-AGTTGAGGGGACTTTCCCAGGG-3,(
R2161 T2511 T2512 arg1Of 5′-AGTTGAGGGGACTTTCCCAGGG-3,′
R2162 T2513 T2510 arg3Of ),(
R2163 T2515 T2514 arg2Of end-labeled,was
R2164 T2515 T2519 modOf end-labeled,using
R2165 T2518 T2515 arg1Of kinase,end-labeled
R2166 T2518 T2516 arg2Of kinase,by
R2167 T2518 T2517 arg1Of kinase,T4-polynucleotide
R2168 T2521 T2520 arg2Of γ-32P,[
R2169 T2522 T2520 arg3Of ],[
R2170 T2523 T2519 arg2Of -ATP,using
R2171 T2523 T2520 arg1Of -ATP,[
R2172 T2523 T2524 arg1Of -ATP,(
R2173 T2526 T2524 arg2Of Biotech.,(
R2174 T2526 T2525 arg1Of Biotech.,Free
R2175 T2526 T2527 arg1Of Biotech.,","
R2176 T2528 T2527 arg2Of Beijing,","
R2177 T2528 T2529 arg1Of Beijing,","
R2178 T2530 T2529 arg2Of China,","
R2179 T2531 T2524 arg3Of ),(
R2180 T2533 T2532 arg1Of protein,Nuclear
R2181 T2533 T2534 arg1Of protein,(
R2182 T2533 T2538 arg1Of protein,was
R2183 T2533 T2539 arg2Of protein,preincubated
R2184 T2536 T2534 arg2Of μg,(
R2185 T2536 T2535 arg1Of μg,20
R2186 T2537 T2534 arg3Of ),(
R2187 T2539 T2538 arg2Of preincubated,was
R2188 T2539 T2540 arg1Of preincubated,in
R2189 T2544 T2540 arg2Of buffer,in
R2190 T2544 T2541 arg1Of buffer,20
R2191 T2544 T2542 arg1Of buffer,μL
R2192 T2544 T2543 arg1Of buffer,binding
R2193 T2544 T2545 arg1Of buffer,containing
R2194 T2546 T2547 arg1Of 10,mM
R2195 T2548 T2546 arg1Of Tris-HCl,10
R2196 T2548 T2549 arg1Of Tris-HCl,(
R2197 T2548 T2553 arg1Of Tris-HCl,","
R2198 T2550 T2549 arg2Of PH,(
R2199 T2550 T2551 arg1Of PH,7.5
R2200 T2552 T2549 arg3Of ),(
R2201 T2553 T2557 arg1Of ",",","
R2202 T2554 T2555 arg1Of 1,mM
R2203 T2556 T2553 arg2Of MgCl2,","
R2204 T2556 T2554 arg1Of MgCl2,1
R2205 T2557 T2561 arg1Of ",",","
R2206 T2558 T2559 arg1Of 0.5,mM
R2207 T2560 T2557 arg2Of NaCl,","
R2208 T2560 T2558 arg1Of NaCl,0.5
R2209 T2561 T2545 arg2Of ",",containing
R2210 T2562 T2563 arg1Of 4,%
R2211 T2564 T2562 arg1Of glycerol,4
R2212 T2564 T2565 arg1Of glycerol,","
R2213 T2565 T2569 arg1Of ",",","
R2214 T2566 T2567 arg1Of 0.5,mM
R2215 T2568 T2565 arg2Of EDTA,","
R2216 T2568 T2566 arg1Of EDTA,0.5
R2217 T2569 T2574 arg1Of ",",and
R2218 T2570 T2571 arg1Of 0.5,mM
R2219 T2572 T2569 arg2Of DTT,","
R2220 T2572 T2570 arg1Of DTT,0.5
R2221 T2574 T2561 arg2Of and,","
R2222 T2574 T2573 arg1Of and,","
R2223 T2578 T2574 arg2Of dI-dC,and
R2224 T2578 T2575 arg1Of dI-dC,2
R2225 T2578 T2576 arg1Of dI-dC,μg
R2226 T2578 T2577 arg1Of dI-dC,poly
R2227 T2578 T2579 arg1Of dI-dC,for
R2228 T2581 T2579 arg2Of minutes,for
R2229 T2581 T2580 arg1Of minutes,20
R2230 T2581 T2582 arg1Of minutes,on
R2231 T2583 T2582 arg2Of ice,on
R2232 T2585 T2584 arg2Of addition,After
R2233 T2585 T2586 arg1Of addition,of
R2234 T2592 T2586 arg2Of probe,of
R2235 T2592 T2587 arg1Of probe,the
R2236 T2592 T2588 arg1Of probe,1
R2237 T2592 T2589 arg1Of probe,μL
R2238 T2592 T2590 arg1Of probe,32P-labled
R2239 T2592 T2591 arg1Of probe,oligonucleotide
R2240 T2595 T2594 arg1Of incubation,the
R2241 T2595 T2596 arg1Of incubation,was
R2242 T2595 T2597 arg2Of incubation,continued
R2243 T2597 T2584 arg1Of continued,After
R2244 T2597 T2593 arg1Of continued,","
R2245 T2597 T2596 arg2Of continued,was
R2246 T2597 T2598 arg1Of continued,for
R2247 T2600 T2598 arg2Of minutes,for
R2248 T2600 T2599 arg1Of minutes,20
R2249 T2600 T2601 arg1Of minutes,on
R2250 T2602 T2601 arg2Of ice,on
R2251 T2605 T2603 arg1Of complexes,The
R2252 T2605 T2604 arg1Of complexes,DNA-protein
R2253 T2605 T2606 arg1Of complexes,were
R2254 T2605 T2607 arg2Of complexes,separated
R2255 T2607 T2606 arg2Of separated,were
R2256 T2607 T2610 arg1Of separated,on
R2257 T2607 T2616 arg1Of separated,in
R2258 T2609 T2607 arg1Of electrophoresis,separated
R2259 T2609 T2608 arg2Of electrophoresis,by
R2260 T2611 T2612 arg1Of 4,%
R2261 T2615 T2610 arg2Of gel,on
R2262 T2615 T2611 arg1Of gel,4
R2263 T2615 T2613 arg1Of gel,nondenaturing
R2264 T2615 T2614 arg1Of gel,polyacrylamide
R2265 T2620 T2616 arg2Of buffer,in
R2266 T2620 T2617 arg1Of buffer,0.5
R2267 T2620 T2618 arg1Of buffer,×
R2268 T2620 T2619 arg1Of buffer,TBE
R2269 T2620 T2621 arg1Of buffer,(
R2270 T2620 T2624 arg1Of buffer,at
R2271 T2622 T2621 arg2Of tris-borate-EDTA,(
R2272 T2623 T2621 arg3Of ),(
R2273 T2626 T2624 arg2Of V,at
R2274 T2626 T2625 arg1Of V,390
R2275 T2626 T2627 arg1Of V,for
R2276 T2629 T2627 arg2Of hour,for
R2277 T2629 T2628 arg1Of hour,1
R2278 T2629 T2630 arg1Of hour,at
R2279 T2631 T2630 arg2Of 4°C,at
R2280 T2633 T2632 arg2Of electrophoresis,After
R2281 T2636 T2635 arg1Of gel,the
R2282 T2636 T2637 arg1Of gel,was
R2283 T2636 T2638 arg2Of gel,dried
R2284 T2636 T2640 arg2Of gel,exposed
R2285 T2638 T2639 arg1Of dried,and
R2286 T2639 T2632 arg1Of and,After
R2287 T2639 T2634 arg1Of and,","
R2288 T2639 T2637 arg2Of and,was
R2289 T2640 T2639 arg2Of exposed,and
R2290 T2640 T2641 arg1Of exposed,to
R2291 T2643 T2641 arg2Of film,to
R2292 T2643 T2642 arg1Of film,X-ray
R2293 T2643 T2644 arg1Of film,(
R2294 T2646 T2644 arg2Of Hyperfilm,(
R2295 T2646 T2645 arg1Of Hyperfilm,Fuji
R2296 T2646 T2647 arg1Of Hyperfilm,","
R2297 T2648 T2647 arg2Of Tokyo,","
R2298 T2648 T2649 arg1Of Tokyo,","
R2299 T2650 T2649 arg2Of Japan,","
R2300 T2651 T2644 arg3Of ),(
R2301 T2652 T2653 arg1Of Levels,of
R2302 T2652 T2658 arg1Of Levels,were
R2303 T2652 T2659 arg2Of Levels,quantified
R2304 T2657 T2653 arg2Of activity,of
R2305 T2657 T2654 arg1Of activity,NF-κB
R2306 T2657 T2655 arg1Of activity,DNA
R2307 T2657 T2656 arg1Of activity,binding
R2308 T2659 T2658 arg2Of quantified,were
R2309 T2662 T2659 arg1Of ImageJ,quantified
R2310 T2662 T2660 arg2Of ImageJ,by
R2311 T2662 T2661 arg1Of ImageJ,software
R2677 T3118 T3117 arg1Of RNA,Total
R2678 T3118 T3119 arg1Of RNA,was
R2679 T3118 T3120 arg2Of RNA,isolated
R2680 T3120 T3119 arg2Of isolated,was
R2681 T3120 T3121 arg1Of isolated,with
R2682 T3120 T3131 arg1Of isolated,and
R2683 T3122 T3121 arg2Of Trizol,with
R2684 T3122 T3123 arg1Of Trizol,(
R2685 T3124 T3123 arg2Of Invitrogen,(
R2686 T3124 T3125 arg1Of Invitrogen,","
R2687 T3124 T3127 arg1Of Invitrogen,","
R2688 T3124 T3128 arg1Of Invitrogen,USA
R2689 T3126 T3125 arg2Of CA,","
R2690 T3129 T3123 arg3Of ),(
R2691 T3131 T3130 arg1Of and,","
R2692 T3131 T3145 arg1Of and,(
R2693 T3131 T3152 arg1Of and,according
R2694 T3133 T3132 arg1Of cDNA,single-stranded
R2695 T3133 T3134 arg1Of cDNA,was
R2696 T3133 T3135 arg2Of cDNA,synthesized
R2697 T3135 T3131 arg2Of synthesized,and
R2698 T3135 T3134 arg2Of synthesized,was
R2699 T3135 T3136 arg1Of synthesized,from
R2700 T3138 T3136 arg2Of μg,from
R2701 T3138 T3137 arg1Of μg,2
R2702 T3138 T3139 arg1Of μg,of
R2703 T3141 T3139 arg2Of RNA,of
R2704 T3141 T3140 arg1Of RNA,total
R2705 T3141 T3142 arg1Of RNA,with
R2706 T3144 T3142 arg2Of RT-Kit,with
R2707 T3144 T3143 arg1Of RT-Kit,BU-Script
R2708 T3146 T3147 arg1Of Biunique,","
R2709 T3147 T3145 arg2Of ",",(
R2710 T3147 T3149 arg1Of ",",","
R2711 T3148 T3147 arg2Of Jiangsu,","
R2712 T3150 T3149 arg2Of China,","
R2713 T3151 T3145 arg3Of ),(
R2714 T3153 T3152 arg2Of to,according
R2715 T3155 T3154 arg1Of manufacturer,the
R2716 T3155 T3156 arg2Of manufacturer,'s
R2717 T3157 T3153 arg2Of protocol,to
R2718 T3157 T3156 arg1Of protocol,'s
R2719 T3159 T3158 arg1Of cDNA,The
R2720 T3159 T3160 arg1Of cDNA,was
R2721 T3159 T3161 arg2Of cDNA,stored
R2722 T3161 T3160 arg2Of stored,was
R2723 T3161 T3162 arg1Of stored,in
R2724 T3163 T3162 arg2Of −20°C,in
R2725 T3165 T3164 arg1Of transcription,Reverse
R2726 T3165 T3166 arg1Of transcription,was
R2727 T3165 T3167 arg2Of transcription,conducted
R2728 T3167 T3166 arg2Of conducted,was
R2729 T3167 T3168 arg1Of conducted,with
R2730 T3167 T3180 arg1Of conducted,according
R2731 T3172 T3168 arg2Of Mix,with
R2732 T3172 T3169 arg1Of Mix,GoTaq
R2733 T3172 T3170 arg1Of Mix,Green
R2734 T3172 T3171 arg1Of Mix,Master
R2735 T3172 T3173 arg1Of Mix,(
R2736 T3174 T3175 arg1Of Promega,","
R2737 T3175 T3173 arg2Of ",",(
R2738 T3175 T3177 arg1Of ",",","
R2739 T3175 T3178 arg1Of ",",USA
R2740 T3176 T3175 arg2Of WI,","
R2741 T3179 T3173 arg3Of ),(
R2742 T3181 T3180 arg2Of to,according
R2743 T3183 T3182 arg1Of manufacturer,the
R2744 T3183 T3184 arg2Of manufacturer,'s
R2745 T3185 T3181 arg2Of protocol,to
R2746 T3185 T3184 arg1Of protocol,'s
R2747 T3187 T3186 arg1Of 1,Table
R2748 T3187 T3188 arg1Of 1,shows
R2749 T3190 T3191 arg1Of primers,and
R2750 T3191 T3188 arg2Of and,shows
R2751 T3191 T3189 arg1Of and,the
R2752 T3193 T3191 arg2Of parameters,and
R2753 T3193 T3192 arg1Of parameters,PCR
R2754 T3195 T3194 arg1Of products,PCR
R2755 T3195 T3196 arg1Of products,were
R2756 T3195 T3197 arg2Of products,detected
R2757 T3197 T3196 arg2Of detected,were
R2758 T3201 T3197 arg1Of electrophoresis,detected
R2759 T3201 T3198 arg2Of electrophoresis,by
R2760 T3201 T3199 arg1Of electrophoresis,agarose
R2761 T3201 T3200 arg1Of electrophoresis,gel
R2762 T3203 T3202 arg1Of intensity,The
R2763 T3203 T3204 arg1Of intensity,of
R2764 T3203 T3207 arg1Of intensity,was
R2765 T3203 T3208 arg2Of intensity,analyzed
R2766 T3206 T3204 arg2Of bands,of
R2767 T3206 T3205 arg1Of bands,the
R2768 T3208 T3207 arg2Of analyzed,was
R2769 T3211 T3208 arg1Of program,analyzed
R2770 T3211 T3209 arg2Of program,by
R2771 T3211 T3210 arg1Of program,ImageJ
R2772 T3213 T3212 arg1Of level,The
R2773 T3213 T3214 arg1Of level,of
R2774 T3213 T3216 arg1Of level,was
R2775 T3213 T3217 arg2Of level,used
R2776 T3215 T3214 arg2Of β-actin,of
R2777 T3217 T3216 arg2Of used,was
R2778 T3217 T3218 arg1Of used,as
R2779 T3221 T3218 arg2Of standard,as
R2780 T3221 T3219 arg1Of standard,an
R2781 T3221 T3220 arg1Of standard,internal
R2899 T3391 T3390 arg1Of Blot,Western
R2900 T3393 T3392 arg1Of obtain,To
R2901 T3396 T3393 arg2Of lysates,obtain
R2902 T3396 T3394 arg1Of lysates,total
R2903 T3396 T3395 arg1Of lysates,protein
R2904 T3398 T3393 arg1Of cells,obtain
R2905 T3398 T3399 arg1Of cells,were
R2906 T3398 T3400 arg2Of cells,homogenized
R2907 T3398 T3452 arg2Of cells,centrifuged
R2908 T3400 T3401 arg1Of homogenized,in
R2909 T3400 T3451 arg1Of homogenized,and
R2910 T3403 T3401 arg2Of buffer,in
R2911 T3403 T3402 arg1Of buffer,RIPA
R2912 T3403 T3404 arg1Of buffer,(
R2913 T3405 T3406 arg1Of 1,%
R2914 T3407 T3405 arg1Of NP40,1
R2915 T3407 T3408 arg1Of NP40,","
R2916 T3408 T3413 arg1Of ",",","
R2917 T3409 T3410 arg1Of 0.5,%
R2918 T3412 T3408 arg2Of deoxycholate,","
R2919 T3412 T3409 arg1Of deoxycholate,0.5
R2920 T3412 T3411 arg1Of deoxycholate,sodium
R2921 T3413 T3417 arg1Of ",",","
R2922 T3414 T3415 arg1Of 0.1,%
R2923 T3416 T3413 arg2Of SDS,","
R2924 T3416 T3414 arg1Of SDS,0.1
R2925 T3417 T3421 arg1Of ",",","
R2926 T3418 T3419 arg1Of 1,mM
R2927 T3420 T3417 arg2Of EDTA,","
R2928 T3420 T3418 arg1Of EDTA,1
R2929 T3421 T3404 arg2Of ",",(
R2930 T3421 T3425 arg1Of ",",","
R2931 T3422 T3423 arg1Of 1,mM
R2932 T3424 T3421 arg2Of EGTA,","
R2933 T3424 T3422 arg1Of EGTA,1
R2934 T3426 T3427 arg1Of 1,mM
R2935 T3428 T3426 arg1Of Na3VO4,1
R2936 T3428 T3429 arg1Of Na3VO4,","
R2937 T3429 T3433 arg1Of ",",","
R2938 T3430 T3431 arg1Of 20,mM
R2939 T3432 T3429 arg2Of NaF,","
R2940 T3432 T3430 arg1Of NaF,20
R2941 T3433 T3437 arg1Of ",",","
R2942 T3434 T3435 arg1Of 0.5,mM
R2943 T3436 T3433 arg2Of DTT,","
R2944 T3436 T3434 arg1Of DTT,0.5
R2945 T3437 T3442 arg1Of ",",and
R2946 T3438 T3439 arg1Of 1,mM
R2947 T3440 T3437 arg2Of PMSF,","
R2948 T3440 T3438 arg1Of PMSF,1
R2949 T3442 T3425 arg2Of and,","
R2950 T3442 T3441 arg1Of and,","
R2951 T3445 T3442 arg2Of cocktail,and
R2952 T3445 T3443 arg1Of cocktail,protease
R2953 T3445 T3444 arg1Of cocktail,inhibitor
R2954 T3445 T3446 arg1Of cocktail,in
R2955 T3448 T3446 arg2Of pH,in
R2956 T3448 T3447 arg1Of pH,PBS
R2957 T3448 T3449 arg1Of pH,7.4
R2958 T3450 T3404 arg3Of ),(
R2959 T3451 T3392 modOf and,To
R2960 T3451 T3397 arg1Of and,","
R2961 T3451 T3399 arg2Of and,were
R2962 T3452 T3451 arg2Of centrifuged,and
R2963 T3452 T3453 arg1Of centrifuged,at
R2964 T3452 T3456 arg1Of centrifuged,for
R2965 T3455 T3453 arg2Of g,at
R2966 T3455 T3454 arg1Of g,"12,000"
R2967 T3458 T3456 arg2Of min,for
R2968 T3458 T3457 arg1Of min,15
R2969 T3458 T3459 arg1Of min,at
R2970 T3460 T3459 arg2Of 4°C,at
R2971 T3462 T3461 arg1Of concentrations,Protein
R2972 T3462 T3463 arg1Of concentrations,were
R2973 T3462 T3464 arg2Of concentrations,estimated
R2974 T3464 T3463 arg2Of estimated,were
R2975 T3470 T3464 arg1Of Reagent,estimated
R2976 T3470 T3465 arg2Of Reagent,by
R2977 T3470 T3466 arg1Of Reagent,Coomassie
R2978 T3470 T3467 arg1Of Reagent,Plus
R2979 T3470 T3468 arg1Of Reagent,Protein
R2980 T3470 T3469 arg1Of Reagent,Assay
R2981 T3470 T3471 arg1Of Reagent,(
R2982 T3472 T3473 arg1Of Pierce,","
R2983 T3473 T3471 arg2Of ",",(
R2984 T3473 T3475 arg1Of ",",","
R2985 T3474 T3473 arg2Of IL,","
R2986 T3476 T3475 arg2Of USA,","
R2987 T3477 T3471 arg3Of ),(
R2988 T3479 T3478 arg1Of micrograms,Fifty
R2989 T3479 T3480 arg1Of micrograms,of
R2990 T3479 T3486 arg1Of micrograms,were
R2991 T3479 T3487 arg1Of micrograms,heat-denatured
R2992 T3479 T3506 arg1Of micrograms,electroblotted
R2993 T3485 T3480 arg2Of extracts,of
R2994 T3485 T3481 arg1Of extracts,the
R2995 T3485 T3482 arg1Of extracts,resulting
R2996 T3485 T3483 arg1Of extracts,cytosolic
R2997 T3485 T3484 arg1Of extracts,protein
R2998 T3486 T3488 arg1Of were,in
R2999 T3486 T3505 arg1Of were,and
R3000 T3487 T3486 arg2Of heat-denatured,were
R3001 T3492 T3488 arg2Of buffer,in
R3002 T3492 T3489 arg1Of buffer,Laemmli
R3003 T3492 T3490 arg1Of buffer,sample
R3004 T3492 T3491 arg1Of buffer,loading
R3005 T3492 T3493 arg1Of buffer,","
R3006 T3492 T3494 arg2Of buffer,separated
R3007 T3496 T3497 arg1Of 10,%
R3008 T3503 T3494 arg1Of electrophoresis,separated
R3009 T3503 T3495 arg2Of electrophoresis,by
R3010 T3503 T3496 arg1Of electrophoresis,10
R3011 T3503 T3498 arg1Of electrophoresis,sodium
R3012 T3503 T3499 arg1Of electrophoresis,dodecyl
R3013 T3503 T3500 arg1Of electrophoresis,sulfate
R3014 T3503 T3501 arg1Of electrophoresis,polyacrylamide
R3015 T3503 T3502 arg1Of electrophoresis,gel
R3016 T3505 T3504 arg1Of and,","
R3017 T3506 T3505 arg2Of electroblotted,and
R3018 T3506 T3507 arg1Of electroblotted,onto
R3019 T3510 T3507 arg2Of membrane,onto
R3020 T3510 T3508 arg1Of membrane,a
R3021 T3510 T3509 arg1Of membrane,nitrocellulose
R3022 T3512 T3511 arg2Of immunoblotting,For
R3023 T3514 T3515 arg1Of membranes,were
R3024 T3514 T3516 arg2Of membranes,blocked
R3025 T3516 T3511 arg1Of blocked,For
R3026 T3516 T3513 arg1Of blocked,","
R3027 T3516 T3515 arg2Of blocked,were
R3028 T3516 T3517 arg1Of blocked,with
R3029 T3516 T3526 arg1Of blocked,overnight
R3030 T3516 T3527 arg1Of blocked,at
R3031 T3516 T3530 arg1Of blocked,and
R3032 T3518 T3519 arg1Of 5,%
R3033 T3522 T3517 arg2Of milk,with
R3034 T3522 T3518 arg1Of milk,5
R3035 T3522 T3520 arg1Of milk,nonfat
R3036 T3522 T3521 arg1Of milk,dry
R3037 T3522 T3523 arg1Of milk,in
R3038 T3525 T3523 arg2Of buffer,in
R3039 T3525 T3524 arg1Of buffer,saline
R3040 T3528 T3527 arg2Of 4°C,at
R3041 T3530 T3529 arg1Of and,","
R3042 T3530 T3536 arg1Of and,:
R3043 T3530 T3537 arg1Of and,anti-β-actin
R3044 T3530 T3554 arg1Of and,metallopeptidase
R3045 T3533 T3531 arg1Of antibodies,the
R3046 T3533 T3532 arg1Of antibodies,following
R3047 T3533 T3534 arg1Of antibodies,were
R3048 T3533 T3535 arg2Of antibodies,used
R3049 T3535 T3530 arg2Of used,and
R3050 T3535 T3534 arg2Of used,were
R3051 T3537 T3538 arg1Of anti-β-actin,(
R3052 T3537 T3552 arg1Of anti-β-actin,and
R3053 T3539 T3540 arg1Of sc-130657,","
R3054 T3539 T3544 arg1Of sc-130657,","
R3055 T3543 T3540 arg2Of Biotechnology,","
R3056 T3543 T3541 arg1Of Biotechnology,Santa
R3057 T3543 T3542 arg1Of Biotechnology,Cruz
R3058 T3544 T3538 arg2Of ",",(
R3059 T3545 T3546 arg1Of CA,","
R3060 T3546 T3548 arg1Of ",",","
R3061 T3547 T3546 arg2Of USA,","
R3062 T3549 T3548 arg2Of 43,","
R3063 T3550 T3544 arg2Of kDa,","
R3064 T3550 T3545 arg1Of kDa,CA
R3065 T3550 T3547 arg1Of kDa,USA
R3066 T3550 T3549 arg1Of kDa,43
R3067 T3551 T3538 arg3Of ),(
R3068 T3552 T3559 arg1Of and,(
R3069 T3554 T3552 arg2Of metallopeptidase,and
R3070 T3554 T3553 arg1Of metallopeptidase,anti-matrix
R3071 T3554 T3555 arg1Of metallopeptidase,9
R3072 T3554 T3556 arg1Of metallopeptidase,(
R3073 T3557 T3556 arg2Of MMP9,(
R3074 T3558 T3556 arg3Of ),(
R3075 T3560 T3561 arg1Of sc-6841,","
R3076 T3560 T3565 arg1Of sc-6841,","
R3077 T3564 T3561 arg2Of Biotechnology,","
R3078 T3564 T3562 arg1Of Biotechnology,Santa
R3079 T3564 T3563 arg1Of Biotechnology,Cruz
R3080 T3565 T3559 arg2Of ",",(
R3081 T3566 T3567 arg1Of CA,","
R3082 T3567 T3569 arg1Of ",",","
R3083 T3568 T3567 arg2Of USA,","
R3084 T3570 T3569 arg2Of 92,","
R3085 T3571 T3565 arg2Of kDa,","
R3086 T3571 T3566 arg1Of kDa,CA
R3087 T3571 T3568 arg1Of kDa,USA
R3088 T3571 T3570 arg1Of kDa,92
R3089 T3572 T3559 arg3Of ),(
R3090 T3575 T3573 arg1Of antibody,Each
R3091 T3575 T3574 arg1Of antibody,primary
R3092 T3575 T3576 arg1Of antibody,was
R3093 T3575 T3577 arg2Of antibody,diluted
R3094 T3575 T3584 arg1Of antibody,added
R3095 T3577 T3576 arg2Of diluted,was
R3096 T3577 T3578 arg1Of diluted,appropriately
R3097 T3577 T3579 arg1Of diluted,in
R3098 T3577 T3582 arg1Of diluted,and
R3099 T3580 T3579 arg2Of blocking,in
R3100 T3581 T3580 arg2Of buffer,blocking
R3101 T3584 T3582 arg2Of added,and
R3102 T3584 T3583 arg1Of added,then
R3103 T3584 T3585 arg1Of added,to
R3104 T3584 T3588 arg1Of added,for
R3105 T3587 T3585 arg2Of blots,to
R3106 T3587 T3586 arg1Of blots,the
R3107 T3590 T3588 arg2Of h,for
R3108 T3590 T3589 arg1Of h,1
R3109 T3590 T3591 arg1Of h,at
R3110 T3593 T3591 arg2Of temperature,at
R3111 T3593 T3592 arg1Of temperature,room
R3112 T3595 T3594 arg1Of blots,The
R3113 T3595 T3596 arg1Of blots,were
R3114 T3595 T3597 arg2Of blots,washed
R3115 T3595 T3605 arg2Of blots,covered
R3116 T3597 T3599 arg1Of washed,times
R3117 T3597 T3600 arg1Of washed,in
R3118 T3597 T3604 arg1Of washed,and
R3119 T3599 T3598 arg1Of times,three
R3120 T3603 T3600 arg2Of buffer,in
R3121 T3603 T3601 arg1Of buffer,the
R3122 T3603 T3602 arg1Of buffer,washing
R3123 T3604 T3596 arg2Of and,were
R3124 T3605 T3604 arg2Of covered,and
R3125 T3605 T3606 arg1Of covered,with
R3126 T3605 T3612 arg1Of covered,at
R3127 T3611 T3606 arg2Of antibody,with
R3128 T3611 T3607 arg1Of antibody,the
R3129 T3611 T3608 arg1Of antibody,horseradish
R3130 T3611 T3609 arg1Of antibody,peroxidase-linked
R3131 T3611 T3610 arg1Of antibody,secondary
R3132 T3614 T3612 arg2Of 1,at
R3133 T3614 T3613 arg1Of 1,a
R3134 T3614 T3615 arg1Of 1,:
R3135 T3617 T3616 arg1Of dilution,2000
R3136 T3617 T3618 arg1Of dilution,for
R3137 T3620 T3618 arg2Of h,for
R3138 T3620 T3619 arg1Of h,1
R3139 T3621 T3622 arg1Of Blots,were
R3140 T3621 T3623 arg2Of Blots,incubated
R3141 T3621 T3641 arg2Of Blots,exposed
R3142 T3623 T3624 arg1Of incubated,with
R3143 T3623 T3640 arg1Of incubated,and
R3144 T3628 T3627 arg2Of ECL,(
R3145 T3629 T3627 arg3Of ),(
R3146 T3631 T3624 arg2Of system,with
R3147 T3631 T3625 arg2Of system,enhanced
R3148 T3631 T3626 arg1Of system,chemiluminescence
R3149 T3631 T3627 arg1Of system,(
R3150 T3631 T3630 arg1Of system,detection
R3151 T3631 T3632 arg1Of system,(
R3152 T3634 T3632 arg2Of Biosciences,(
R3153 T3634 T3633 arg1Of Biosciences,Amersham
R3154 T3634 T3635 arg1Of Biosciences,","
R3155 T3636 T3635 arg2Of Bucks,","
R3156 T3636 T3637 arg1Of Bucks,","
R3157 T3638 T3637 arg2Of UK,","
R3158 T3639 T3632 arg3Of ),(
R3159 T3640 T3622 arg2Of and,were
R3160 T3641 T3640 arg2Of exposed,and
R3161 T3641 T3642 arg1Of exposed,to
R3162 T3644 T3642 arg2Of film,to
R3163 T3644 T3643 arg1Of film,radiographic
R3164 T3644 T3645 arg1Of film,(
R3165 T3647 T3645 arg2Of Hyperfilm,(
R3166 T3647 T3646 arg1Of Hyperfilm,Fuji
R3167 T3647 T3648 arg1Of Hyperfilm,","
R3168 T3649 T3648 arg2Of Tokyo,","
R3169 T3649 T3650 arg1Of Tokyo,","
R3170 T3651 T3650 arg2Of Japan,","
R3171 T3652 T3645 arg3Of ),(
R3172 T3653 T3654 arg1Of ImageJ,was
R3173 T3653 T3655 arg2Of ImageJ,used
R3174 T3655 T3654 arg2Of used,was
R3175 T3657 T3655 arg3Of analyze,used
R3176 T3657 T3656 arg1Of analyze,to
R3177 T3659 T3657 arg2Of intensity,analyze
R3178 T3659 T3658 arg1Of intensity,the
R3179 T3659 T3660 arg1Of intensity,of
R3180 T3662 T3660 arg2Of blots,of
R3181 T3662 T3661 arg1Of blots,the
R3182 T3664 T3663 arg1Of level,The
R3183 T3664 T3665 arg1Of level,of
R3184 T3664 T3667 arg1Of level,was
R3185 T3664 T3668 arg2Of level,used
R3186 T3666 T3665 arg2Of β-actin,of
R3187 T3668 T3667 arg2Of used,was
R3188 T3668 T3669 arg1Of used,as
R3189 T3671 T3669 arg2Of standard,as
R3190 T3671 T3670 arg1Of standard,internal
R3492 T4084 T4082 arg1Of Assay,Enzyme-Linked
R3493 T4084 T4083 arg1Of Assay,Immunosorbent
R3494 T4084 T4085 arg1Of Assay,(
R3495 T4086 T4085 arg2Of ELISA,(
R3496 T4087 T4085 arg3Of ),(
R3497 T4088 T4089 arg1Of Cells,of
R3498 T4088 T4092 arg1Of Cells,were
R3499 T4088 T4093 arg2Of Cells,homogenized
R3500 T4091 T4089 arg2Of groups,of
R3501 T4091 T4090 arg1Of groups,four
R3502 T4093 T4092 arg2Of homogenized,were
R3503 T4093 T4094 arg1Of homogenized,as
R3504 T4095 T4094 arg2Of mentioned,as
R3505 T4095 T4096 arg1Of mentioned,above
R3506 T4098 T4097 arg1Of supernatant,The
R3507 T4098 T4099 arg1Of supernatant,was
R3508 T4098 T4100 arg2Of supernatant,collected
R3509 T4100 T4099 arg2Of collected,was
R3510 T4100 T4102 arg1Of collected,and
R3511 T4102 T4101 arg1Of and,","
R3512 T4104 T4103 arg1Of protein,total
R3513 T4104 T4105 arg1Of protein,was
R3514 T4104 T4106 arg2Of protein,determined
R3515 T4106 T4102 arg2Of determined,and
R3516 T4106 T4105 arg2Of determined,was
R3517 T4112 T4106 arg1Of Reagent,determined
R3518 T4112 T4107 arg2Of Reagent,by
R3519 T4112 T4108 arg1Of Reagent,Coomassie
R3520 T4112 T4109 arg1Of Reagent,Plus
R3521 T4112 T4110 arg1Of Reagent,Protein
R3522 T4112 T4111 arg1Of Reagent,Assay
R3523 T4112 T4113 arg1Of Reagent,(
R3524 T4114 T4115 arg1Of Pierce,","
R3525 T4115 T4113 arg2Of ",",(
R3526 T4115 T4117 arg1Of ",",","
R3527 T4116 T4115 arg2Of IL,","
R3528 T4118 T4117 arg2Of USA,","
R3529 T4119 T4113 arg3Of ),(
R3530 T4120 T4121 arg1Of Levels,of
R3531 T4120 T4135 arg1Of Levels,and
R3532 T4124 T4122 arg1Of factor-alpha,tumor
R3533 T4124 T4123 arg1Of factor-alpha,necrosis
R3534 T4124 T4125 arg1Of factor-alpha,(
R3535 T4124 T4128 arg1Of factor-alpha,","
R3536 T4126 T4125 arg2Of TNF-α,(
R3537 T4127 T4125 arg3Of ),(
R3538 T4128 T4121 arg2Of ",",of
R3539 T4130 T4128 arg2Of beta,","
R3540 T4130 T4129 arg1Of beta,interleukin-1
R3541 T4130 T4131 arg1Of beta,(
R3542 T4132 T4131 arg2Of IL-1β,(
R3543 T4133 T4131 arg3Of ),(
R3544 T4135 T4134 arg1Of and,","
R3545 T4136 T4135 arg2Of interleukin-6,and
R3546 T4136 T4137 arg1Of interleukin-6,(
R3547 T4136 T4141 modOf interleukin-6,were
R3548 T4138 T4137 arg2Of IL-6,(
R3549 T4139 T4137 arg3Of ),(
R3550 T4140 T4141 arg1Of protein,were
R3551 T4140 T4142 arg2Of protein,quantified
R3552 T4142 T4141 arg2Of quantified,were
R3553 T4143 T4142 arg3Of using,quantified
R3554 T4143 T4149 arg1Of using,according
R3555 T4145 T4143 arg2Of kits,using
R3556 T4145 T4144 arg1Of kits,ELISA
R3557 T4145 T4146 arg1Of kits,specific
R3558 T4146 T4147 arg1Of specific,for
R3559 T4148 T4147 arg2Of mouse,for
R3560 T4150 T4149 arg2Of to,according
R3561 T4152 T4151 arg1Of manufacturer,the
R3562 T4152 T4153 arg2Of manufacturer,'s
R3563 T4154 T4150 arg2Of instructions,to
R3564 T4154 T4153 arg1Of instructions,'s
R3565 T4154 T4155 arg1Of instructions,(
R3566 T4159 T4155 arg2Of CA,(
R3567 T4159 T4156 arg1Of CA,Bender
R3568 T4159 T4157 arg1Of CA,MedSystems
R3569 T4159 T4158 arg1Of CA,Inc.
R3570 T4159 T4160 arg1Of CA,","
R3571 T4159 T4161 arg1Of CA,USA
R3572 T4162 T4155 arg3Of ),(
R3573 T4163 T4165 arg1Of Briefly,prepared
R3574 T4163 T4169 arg1Of Briefly,created
R3575 T4165 T4168 arg1Of prepared,and
R3576 T4167 T4165 arg2Of standard,prepared
R3577 T4167 T4166 arg1Of standard,the
R3578 T4168 T4164 arg1Of and,","
R3579 T4169 T4168 arg2Of created,and
R3580 T4172 T4169 arg2Of dilution,created
R3581 T4172 T4170 arg1Of dilution,the
R3582 T4172 T4171 arg1Of dilution,standard
R3583 T4172 T4173 arg1Of dilution,for
R3584 T4174 T4173 arg2Of building,for
R3585 T4176 T4174 arg2Of curve,building
R3586 T4176 T4175 arg1Of curve,standard
R3587 T4178 T4179 arg1Of samples,and
R3588 T4179 T4177 arg1Of and,Then
R3589 T4179 T4181 arg1Of and,were
R3590 T4179 T4182 arg2Of and,added
R3591 T4180 T4179 arg2Of biotinconjugate,and
R3592 T4182 T4181 arg2Of added,were
R3593 T4182 T4183 modOf added,to
R3594 T4184 T4183 arg1Of microwell,to
R3595 T4185 T4184 arg2Of strips,microwell
R3596 T4187 T4188 arg1Of incubated,for
R3597 T4190 T4188 arg2Of h,for
R3598 T4190 T4189 arg1Of h,2
R3599 T4190 T4191 arg1Of h,at
R3600 T4192 T4186 arg1Of room,After
R3601 T4192 T4187 arg2Of room,incubated
R3602 T4193 T4192 arg1Of temperature,room
R3603 T4193 T4194 arg1Of temperature,","
R3604 T4193 T4198 arg1Of temperature,were
R3605 T4193 T4199 arg2Of temperature,washed
R3606 T4197 T4194 arg2Of strips,","
R3607 T4197 T4195 arg1Of strips,the
R3608 T4197 T4196 arg1Of strips,microwell
R3609 T4199 T4198 arg2Of washed,were
R3610 T4199 T4202 arg1Of washed,with
R3611 T4199 T4206 arg1Of washed,and
R3612 T4201 T4199 arg3Of times,washed
R3613 T4201 T4200 arg1Of times,3
R3614 T4204 T4202 arg2Of buffer,with
R3615 T4204 T4203 arg1Of buffer,wash
R3616 T4206 T4205 arg1Of and,","
R3617 T4207 T4208 arg1Of streptavidin-HRP,were
R3618 T4207 T4209 arg2Of streptavidin-HRP,added
R3619 T4209 T4206 arg2Of added,and
R3620 T4209 T4208 arg2Of added,were
R3621 T4209 T4210 arg1Of added,to
R3622 T4212 T4210 arg2Of wells,to
R3623 T4212 T4211 arg1Of wells,all
R3624 T4214 T4215 arg1Of incubated,for
R3625 T4217 T4215 arg2Of h,for
R3626 T4217 T4216 arg1Of h,1
R3627 T4220 T4213 arg1Of strips,After
R3628 T4220 T4214 arg2Of strips,incubated
R3629 T4220 T4218 arg1Of strips,","
R3630 T4220 T4219 arg1Of strips,microwell
R3631 T4220 T4221 arg1Of strips,were
R3632 T4220 T4222 arg2Of strips,washed
R3633 T4222 T4221 arg2Of washed,were
R3634 T4224 T4222 arg3Of times,washed
R3635 T4224 T4223 arg1Of times,3
R3636 T4224 T4225 arg2Of times,followed
R3637 T4225 T4226 arg1Of followed,by
R3638 T4227 T4226 arg2Of adding,by
R3639 T4229 T4227 arg2Of substrate,adding
R3640 T4229 T4228 arg1Of substrate,TMB
R3641 T4231 T4232 arg1Of incubated,for
R3642 T4234 T4230 arg1Of 10–30,After
R3643 T4234 T4231 arg2Of 10–30,incubated
R3644 T4234 T4233 arg1Of 10–30,about
R3645 T4235 T4234 arg1Of min,10–30
R3646 T4235 T4236 arg1Of min,","
R3647 T4235 T4240 arg1Of min,was
R3648 T4235 T4241 arg2Of min,added
R3649 T4239 T4236 arg2Of solution,","
R3650 T4239 T4237 arg1Of solution,the
R3651 T4239 T4238 arg1Of solution,stop
R3652 T4241 T4240 arg2Of added,was
R3653 T4244 T4242 arg1Of intensity,The
R3654 T4244 T4243 arg1Of intensity,colour
R3655 T4244 T4245 arg1Of intensity,was
R3656 T4244 T4246 arg2Of intensity,measured
R3657 T4244 T4250 arg1Of intensity,using
R3658 T4246 T4245 arg2Of measured,was
R3659 T4246 T4247 arg1Of measured,at
R3660 T4246 T4250 modOf measured,using
R3661 T4249 T4247 arg2Of nm,at
R3662 T4249 T4248 arg1Of nm,450
R3663 T4250 T4256 arg1Of using,(
R3664 T4255 T4250 arg2Of reader,using
R3665 T4255 T4251 arg1Of reader,a
R3666 T4255 T4252 arg1Of reader,Bio-Rad
R3667 T4255 T4253 arg1Of reader,ELISA
R3668 T4255 T4254 arg1Of reader,microplate
R3669 T4258 T4256 arg2Of Laboratories,(
R3670 T4258 T4257 arg1Of Laboratories,Bio-Rad
R3671 T4258 T4259 arg1Of Laboratories,","
R3672 T4260 T4259 arg2Of CA,","
R3673 T4260 T4261 arg1Of CA,","
R3674 T4262 T4261 arg2Of USA,","
R3675 T4263 T4256 arg3Of ),(
R3676 T4265 T4264 arg1Of concentration,The
R3677 T4265 T4266 arg1Of concentration,of
R3678 T4265 T4268 arg1Of concentration,was
R3679 T4265 T4269 arg2Of concentration,determined
R3680 T4265 T4276 arg2Of concentration,expressed
R3681 T4267 T4266 arg2Of protein,of
R3682 T4269 T4270 arg1Of determined,according
R3683 T4269 T4275 arg1Of determined,and
R3684 T4271 T4270 arg2Of to,according
R3685 T4274 T4271 arg2Of curve,to
R3686 T4274 T4272 arg1Of curve,the
R3687 T4274 T4273 arg1Of curve,standard
R3688 T4275 T4268 arg2Of and,was
R3689 T4276 T4275 arg2Of expressed,and
R3690 T4276 T4277 arg1Of expressed,as
R3691 T4278 T4277 arg2Of pg/mg,as
R3692 T4278 T4279 arg1Of pg/mg,of
R3693 T4281 T4279 arg2Of protein,of
R3694 T4281 T4280 arg1Of protein,total
R3709 T4394 T4393 arg1Of Zymography,Gelatine
R3710 T4395 T4396 arg1Of Cells,of
R3711 T4395 T4399 arg1Of Cells,were
R3712 T4395 T4400 arg2Of Cells,homogenized
R3713 T4398 T4396 arg2Of groups,of
R3714 T4398 T4397 arg1Of groups,four
R3715 T4400 T4399 arg2Of homogenized,were
R3716 T4400 T4401 arg1Of homogenized,in
R3717 T4400 T4425 arg1Of homogenized,and
R3718 T4403 T4401 arg2Of buffer,in
R3719 T4403 T4402 arg1Of buffer,lysis
R3720 T4403 T4404 arg1Of buffer,containing
R3721 T4405 T4406 arg1Of 50,mM
R3722 T4407 T4405 arg1Of Tris-HCl,50
R3723 T4407 T4408 arg1Of Tris-HCl,(
R3724 T4407 T4412 arg1Of Tris-HCl,","
R3725 T4409 T4408 arg2Of pH,(
R3726 T4409 T4410 arg1Of pH,7.4
R3727 T4411 T4408 arg3Of ),(
R3728 T4412 T4416 arg1Of ",",","
R3729 T4413 T4414 arg1Of 150,mM
R3730 T4415 T4412 arg2Of NaCl,","
R3731 T4415 T4413 arg1Of NaCl,150
R3732 T4416 T4420 arg1Of ",",","
R3733 T4417 T4418 arg1Of 5,mM
R3734 T4419 T4416 arg2Of CaCl2,","
R3735 T4419 T4417 arg1Of CaCl2,5
R3736 T4420 T4404 arg2Of ",",containing
R3737 T4421 T4422 arg1Of 0.2,mM
R3738 T4423 T4420 arg2Of NaN3,","
R3739 T4423 T4421 arg1Of NaN3,0.2
R3740 T4425 T4424 arg1Of and,","
R3741 T4427 T4426 arg1Of %,0.01
R3742 T4429 T4428 arg1Of Soluble,Triton.
R3743 T4430 T4429 arg1Of extracts,Soluble
R3744 T4430 T4431 arg1Of extracts,were
R3745 T4430 T4432 arg2Of extracts,separated
R3746 T4430 T4436 arg2Of extracts,stored
R3747 T4432 T4435 arg1Of separated,and
R3748 T4434 T4432 arg1Of centrifugation,separated
R3749 T4434 T4433 arg2Of centrifugation,by
R3750 T4435 T4425 arg2Of and,and
R3751 T4435 T4427 arg1Of and,%
R3752 T4435 T4431 arg2Of and,were
R3753 T4436 T4435 arg2Of stored,and
R3754 T4436 T4437 arg1Of stored,at
R3755 T4438 T4437 arg2Of –20°C,at
R3756 T4440 T4439 arg1Of zymography,Gelatin
R3757 T4440 T4441 arg1Of zymography,was
R3758 T4440 T4442 arg2Of zymography,performed
R3759 T4442 T4441 arg2Of performed,was
R3760 T4442 T4443 arg1Of performed,according
R3761 T4444 T4443 arg2Of to,according
R3762 T4446 T4445 arg1Of manufacturer,the
R3763 T4446 T4447 arg2Of manufacturer,'s
R3764 T4448 T4444 arg2Of instructions,to
R3765 T4448 T4447 arg1Of instructions,'s
R3766 T4448 T4449 arg1Of instructions,(
R3767 T4452 T4449 arg2Of Inc,(
R3768 T4452 T4450 arg1Of Inc,Genmed
R3769 T4452 T4451 arg1Of Inc,Scientifics
R3770 T4452 T4453 arg1Of Inc,","
R3771 T4454 T4453 arg2Of MA,","
R3772 T4454 T4455 arg1Of MA,","
R3773 T4456 T4455 arg2Of USA,","
R3774 T4457 T4449 arg3Of ),(
R3775 T4464 T4460 arg1Of extracts,40
R3776 T4464 T4461 arg1Of extracts,μg
R3777 T4464 T4462 arg1Of extracts,cytosolic
R3778 T4464 T4463 arg1Of extracts,protein
R3779 T4464 T4465 arg1Of extracts,were
R3780 T4464 T4466 arg2Of extracts,separated
R3781 T4466 T4458 arg1Of separated,Briefly
R3782 T4466 T4459 arg1Of separated,","
R3783 T4466 T4465 arg2Of separated,were
R3784 T4468 T4466 arg1Of electrophoresis,separated
R3785 T4468 T4467 arg2Of electrophoresis,by
R3786 T4471 T4470 arg1Of proteins,the
R3787 T4471 T4472 arg1Of proteins,were
R3788 T4471 T4473 arg2Of proteins,renatured
R3789 T4471 T4483 arg2Of proteins,incubated
R3790 T4473 T4474 arg1Of renatured,by
R3791 T4473 T4481 arg1Of renatured,and
R3792 T4475 T4474 arg2Of incubation,by
R3793 T4475 T4476 arg1Of incubation,in
R3794 T4477 T4478 arg1Of 2.5,%
R3795 T4480 T4476 arg2Of X-100,in
R3796 T4480 T4477 arg1Of X-100,2.5
R3797 T4480 T4479 arg1Of X-100,Triton
R3798 T4481 T4469 arg1Of and,Then
R3799 T4481 T4472 arg2Of and,were
R3800 T4483 T4481 arg2Of incubated,and
R3801 T4483 T4482 arg1Of incubated,then
R3802 T4483 T4484 arg1Of incubated,in
R3803 T4483 T4487 arg1Of incubated,for
R3804 T4483 T4490 arg1Of incubated,at
R3805 T4483 T4492 modOf incubated,to
R3806 T4486 T4484 arg2Of buffer,in
R3807 T4486 T4485 arg1Of buffer,substrate
R3808 T4489 T4487 arg2Of h,for
R3809 T4489 T4488 arg1Of h,40
R3810 T4491 T4490 arg2Of 37°C,at
R3811 T4493 T4492 arg1Of enable,to
R3812 T4495 T4493 arg2Of MMP9,enable
R3813 T4495 T4494 arg1Of MMP9,the
R3814 T4495 T4497 arg1Of MMP9,cleave
R3815 T4497 T4493 arg3Of cleave,enable
R3816 T4497 T4496 arg1Of cleave,to
R3817 T4499 T4497 arg2Of gelatin,cleave
R3818 T4499 T4498 arg1Of gelatin,the
R3819 T4501 T4500 arg2Of rinsing,After
R3820 T4501 T4502 arg1Of rinsing,in
R3821 T4503 T4502 arg2Of water,in
R3822 T4506 T4501 arg1Of gel,rinsing
R3823 T4506 T4505 arg1Of gel,each
R3824 T4506 T4507 arg1Of gel,was
R3825 T4506 T4508 arg2Of gel,stained
R3826 T4506 T4511 arg1Of gel,blue
R3827 T4506 T4516 arg2Of gel,destained
R3828 T4508 T4509 arg1Of stained,with
R3829 T4508 T4515 arg1Of stained,and
R3830 T4510 T4509 arg2Of Coomassie,with
R3831 T4511 T4508 arg3Of blue,stained
R3832 T4511 T4512 arg1Of blue,for
R3833 T4514 T4512 arg2Of h,for
R3834 T4514 T4513 arg1Of h,1
R3835 T4515 T4500 arg1Of and,After
R3836 T4515 T4504 arg1Of and,","
R3837 T4515 T4507 arg2Of and,was
R3838 T4516 T4515 arg2Of destained,and
R3839 T4516 T4517 arg1Of destained,in
R3840 T4518 T4519 arg1Of 50,%
R3841 T4520 T4517 arg2Of methanol,in
R3842 T4520 T4518 arg1Of methanol,50
R3843 T4522 T4521 arg1Of activities,Proteolytic
R3844 T4522 T4523 arg1Of activities,were
R3845 T4522 T4524 arg2Of activities,showed
R3846 T4524 T4523 arg2Of showed,were
R3847 T4527 T4524 arg1Of bands,showed
R3848 T4527 T4525 arg2Of bands,by
R3849 T4527 T4526 arg1Of bands,clear
R3850 T4527 T4528 arg1Of bands,in
R3851 T4530 T4528 arg2Of gel,in
R3852 T4530 T4529 arg1Of gel,blue
R3853 T4530 T4531 arg1Of gel,which
R3854 T4530 T4532 arg1Of gel,indicates
R3855 T4534 T4532 arg2Of lysis,indicates
R3856 T4534 T4533 arg1Of lysis,the
R3857 T4534 T4535 arg1Of lysis,of
R3858 T4537 T4535 arg2Of substrate,of
R3859 T4537 T4536 arg1Of substrate,the
R3860 T4538 T4539 arg1Of Quantification,of
R3861 T4538 T4543 arg1Of Quantification,was
R3862 T4538 T4544 arg2Of Quantification,performed
R3863 T4542 T4539 arg2Of density,of
R3864 T4542 T4540 arg1Of density,MMP9
R3865 T4542 T4541 arg1Of density,band
R3866 T4544 T4543 arg2Of performed,was
R3867 T4544 T4545 arg1Of performed,with
R3868 T4549 T4545 arg2Of ImageJ,with
R3869 T4549 T4546 arg1Of ImageJ,image
R3870 T4549 T4547 arg1Of ImageJ,analysis
R3871 T4549 T4548 arg1Of ImageJ,program
R4043 T4764 T4763 arg1Of Analysis,Statistical
R4044 T4765 T4766 arg1Of Data,were
R4045 T4765 T4767 arg2Of Data,expressed
R4046 T4765 T4773 arg2Of Data,evaluated
R4047 T4767 T4768 arg1Of expressed,as
R4048 T4767 T4772 arg1Of expressed,and
R4049 T4771 T4768 arg2Of SD,as
R4050 T4771 T4769 arg1Of SD,mean
R4051 T4771 T4770 arg1Of SD,±
R4052 T4772 T4766 arg2Of and,were
R4053 T4773 T4772 arg2Of evaluated,and
R4054 T4775 T4776 arg1Of ANOVA,and
R4055 T4777 T4776 arg2Of LSD,and
R4056 T4780 T4773 arg1Of test,evaluated
R4057 T4780 T4774 arg2Of test,by
R4058 T4780 T4775 arg1Of test,ANOVA
R4059 T4780 T4777 arg1Of test,LSD
R4060 T4780 T4778 arg1Of test,multiple
R4061 T4780 T4779 arg1Of test,comparison
R4062 T4782 T4781 arg1Of values,P
R4063 T4782 T4783 arg1Of values,<
R4064 T4782 T4784 arg1Of values,0.05
R4065 T4782 T4785 arg1Of values,were
R4066 T4782 T4786 arg2Of values,considered
R4067 T4782 T4788 arg1Of values,be
R4068 T4782 T4789 arg1Of values,significant
R4069 T4786 T4785 arg2Of considered,were
R4070 T4788 T4786 arg3Of be,considered
R4071 T4788 T4787 arg1Of be,to
R4072 T4789 T4788 arg2Of significant,be
R4073 T4791 T4790 arg1Of analyses,All
R4074 T4791 T4792 arg1Of analyses,were
R4075 T4791 T4793 arg2Of analyses,performed
R4076 T4793 T4792 arg2Of performed,were
R4077 T4793 T4794 arg1Of performed,by
R4078 T4795 T4794 arg2Of using,by
R4079 T4798 T4795 arg2Of software,using
R4080 T4798 T4796 arg1Of software,SPSS
R4081 T4798 T4797 arg1Of software,18.0

bionlp-st-ge-2016-test-proteins

Id Subject Object Predicate Lexical cue
T3109 4964-4971 Protein denotes β-actin
T4389 8514-8518 Protein denotes MMP9
T4388 8258-8262 Protein denotes MMP9
T4071 6837-6841 Protein denotes IL-6
T4070 6822-6835 Protein denotes interleukin-6
T4069 6810-6815 Protein denotes IL-1β
T4068 6790-6808 Protein denotes interleukin-1 beta
T3385 5890-5894 Protein denotes MMP9

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T4291 6834-6839 http://www.uniprot.org/uniprot/P05231 denotes 6 (IL
T4290 6810-6815 http://www.uniprot.org/uniprot/P01584 denotes IL-1β
T4289 6782-6785 http://www.uniprot.org/uniprot/P01375 denotes TNF
T3688 6475-6482 http://www.uniprot.org/uniprot/P60709 denotes β-actin
T3687 5791-5798 http://www.uniprot.org/uniprot/P60709 denotes β-actin
T3226 4964-4971 http://www.uniprot.org/uniprot/P60709 denotes β-actin

UBERON-AE

Id Subject Object Predicate Lexical cue
T3373 5706-5710 http://purl.obolibrary.org/obo/UBERON_0001913 denotes milk
T2308 3440-3445 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T1223 605-611 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissue
T1222 453-459 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissue
T1221 427-434 http://purl.obolibrary.org/obo/UBERON_0000055 denotes vessels
T1220 421-434 http://purl.obolibrary.org/obo/UBERON_0001981 denotes blood vessels
T1219 421-426 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood

GO-BP

Id Subject Object Predicate Lexical cue
T3114 4669-4682 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T3113 4661-4682 http://purl.obolibrary.org/obo/GO_0001171 denotes Reverse transcription
T3112 4466-4468 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T3111 4555-4557 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T3110 4411-4413 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T2046 2484-2486 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T2045 2184-2189 http://purl.obolibrary.org/obo/GO_0016265 denotes death
T1820 1732-1741 http://purl.obolibrary.org/obo/GO_0009058 denotes formation
T2044 1998-2003 http://purl.obolibrary.org/obo/GO_0016265 denotes Death
T2043 2179-2189 http://purl.obolibrary.org/obo/GO_0008219 denotes cell death
T2042 1993-2003 http://purl.obolibrary.org/obo/GO_0008219 denotes Cell Death
T4391 8472-8477 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T4390 7754-7759 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T4080 7560-7562 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T4079 6972-6974 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T4078 6802-6812 http://purl.obolibrary.org/obo/GO_0032611 denotes 1 beta (IL
T4077 6790-6808 http://purl.obolibrary.org/obo/GO_0050702 denotes interleukin-1 beta
T4076 6790-6808 http://purl.obolibrary.org/obo/GO_0050720 denotes interleukin-1 beta
T4075 6759-6767 http://purl.obolibrary.org/obo/GO_0070265 denotes necrosis
T4074 6759-6767 http://purl.obolibrary.org/obo/GO_0019835 denotes necrosis
T4073 6759-6767 http://purl.obolibrary.org/obo/GO_0008219 denotes necrosis
T4072 6759-6767 http://purl.obolibrary.org/obo/GO_0001906 denotes necrosis
T3388 5932-5934 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T3387 5837-5839 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T3386 5549-5556 http://purl.obolibrary.org/obo/GO_0051923 denotes sulfate
T1239 1374-1376 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T1238 590-599 http://purl.obolibrary.org/obo/GO_0007586 denotes digestion

GO-MF

Id Subject Object Predicate Lexical cue
T4284 7560-7562 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T4283 6972-6974 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T4282 6834-6839 http://purl.obolibrary.org/obo/GO_0005138 denotes 6 (IL
T3674 6191-6199 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T3673 5963-5971 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T3672 5764-5774 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T2665 4348-4359 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA binding
T2664 4352-4359 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T2663 3797-3804 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T3676 5932-5934 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T3675 5837-5839 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T1500 1374-1376 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T3224 4466-4468 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T3223 4555-4557 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T3222 4411-4413 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T2163 2484-2486 http://purl.obolibrary.org/obo/GO_0033968 denotes CA

GO-CC

Id Subject Object Predicate Lexical cue
T2168 2206-2210 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T2167 2179-2183 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T3685 6191-6199 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T3684 5963-5971 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T3683 5764-5774 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T3682 6191-6199 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T3681 5963-5971 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T3680 5764-5774 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T3679 5664-5673 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T3678 5634-5642 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T3677 5058-5063 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2670 4075-4092 http://purl.obolibrary.org/obo/GO_0043234 denotes protein complexes
T2669 4071-4092 http://purl.obolibrary.org/obo/GO_0097522 denotes DNA-protein complexes
T2668 4071-4092 http://purl.obolibrary.org/obo/GO_0032993 denotes DNA-protein complexes
T2667 4071-4092 http://purl.obolibrary.org/obo/GO_0001114 denotes DNA-protein complexes
T2666 2743-2747 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T1503 1198-1203 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T1502 934-939 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T1501 918-923 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2166 2132-2136 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T2165 2044-2049 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2164 1993-1997 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T1920 1791-1796 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T1919 1651-1655 http://purl.obolibrary.org/obo/GO_0005623 denotes cell

sentences

Id Subject Object Predicate Lexical cue
T4761 8749-8805 Sentence denotes All analyses were performed by using SPSS 18.0 software.
T4760 8699-8748 Sentence denotes P values <0.05 were considered to be significant.
T4759 8609-8698 Sentence denotes Data were expressed as mean ± SD and evaluated by ANOVA and LSD multiple comparison test.
T4758 8588-8608 Sentence denotes Statistical Analysis
T4757 8583-8587 Sentence denotes 2.9.
T4385 8496-8581 Sentence denotes Quantification of MMP9 band density was performed with image analysis program ImageJ.
T4384 8390-8495 Sentence denotes Proteolytic activities were showed by clear bands in blue gel which indicates the lysis of the substrate.
T4383 8286-8389 Sentence denotes After rinsing in water, each gel was stained with Coomassie blue for 1 h and destained in 50% methanol.
T4382 8120-8285 Sentence denotes Then the proteins were renatured by incubation in 2.5% Triton X-100 and then incubated in substrate buffer for 40 h at 37°C to enable the MMP9 to cleave the gelatin.
T4381 8043-8119 Sentence denotes Briefly, 40 μg cytosolic protein extracts were separated by electrophoresis.
T4380 7713-8042 Sentence denotes Cells of four groups were homogenized in lysis buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 5 mM CaCl2, 0.2 mM NaN3, and 0.01% Triton. Soluble extracts were separated by centrifugation and stored at –20°C. Gelatin zymography was performed according to the manufacturer's instructions (Genmed Scientifics Inc, MA, USA).
T4379 7693-7712 Sentence denotes Gelatine Zymography
T4378 7688-7692 Sentence denotes 2.8.
T4063 7570-7686 Sentence denotes The concentration of protein was determined according to the standard curve and expressed as pg/mg of total protein.
T4062 7453-7569 Sentence denotes The colour intensity was measured at 450 nm using a Bio-Rad ELISA microplate reader (Bio-Rad Laboratories, CA, USA).
T4061 7387-7452 Sentence denotes After incubated for about 10–30 min, the stop solution was added.
T4060 7291-7386 Sentence denotes After incubated for 1 h, microwell strips were washed 3 times followed by adding TMB substrate.
T4059 7141-7290 Sentence denotes After incubated for 2 h at room temperature, the microwell strips were washed 3 times with wash buffer, and streptavidin-HRP were added to all wells.
T4058 7076-7140 Sentence denotes Then samples and biotinconjugate were added to microwell strips.
T4057 6982-7075 Sentence denotes Briefly, prepared the standard and created the standard dilution for building standard curve.
T4056 6743-6981 Sentence denotes Levels of tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6) protein were quantified using ELISA kits specific for mouse according to the manufacturer's instructions (Bender MedSystems Inc. CA, USA).
T4055 6620-6742 Sentence denotes The supernatant was collected, and total protein was determined by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA).
T4054 6562-6619 Sentence denotes Cells of four groups were homogenized as mentioned above.
T4053 6520-6561 Sentence denotes Enzyme-Linked Immunosorbent Assay (ELISA)
T4052 6515-6519 Sentence denotes 2.7.
T3107 4951-5005 Sentence denotes The level of β-actin was used as an internal standard.
T3106 4892-4950 Sentence denotes The intensity of the bands was analyzed by ImageJ program.
T3105 4833-4891 Sentence denotes PCR products were detected by agarose gel electrophoresis.
T3104 4787-4832 Sentence denotes Table 1 shows the primers and PCR parameters.
T3103 4631-4785 Sentence denotes The cDNA was stored in −20°C. Reverse transcription was conducted with GoTaq Green Master Mix (Promega, WI, USA) according to the manufacturer's protocol.
T3102 4418-4630 Sentence denotes Total RNA was isolated with Trizol (Invitrogen, CA, USA), and single-stranded cDNA was synthesized from 2 μg of total RNA with BU-Script RT-Kit (Biunique, Jiangsu, China) according to the manufacturer's protocol.
T3101 4411-4417 Sentence denotes RT-PCR
T3100 4406-4410 Sentence denotes 2.5.
T3383 6462-6513 Sentence denotes The level of β-actin was used as internal standard.
T3382 6407-6461 Sentence denotes ImageJ was used to analyze the intensity of the blots.
T3381 6232-6406 Sentence denotes Blots were incubated with enhanced chemiluminescence (ECL) detection system (Amersham Biosciences, Bucks, UK) and exposed to radiographic film (Fuji Hyperfilm, Tokyo, Japan).
T3380 6074-6231 Sentence denotes The blots were washed three times in the washing buffer and covered with the horseradish peroxidase-linked secondary antibody at a 1 : 2000 dilution for 1 h.
T3379 5950-6073 Sentence denotes Each primary antibody was diluted appropriately in blocking buffer and then added to the blots for 1 h at room temperature.
T2322 4332-4404 Sentence denotes Levels of NF-κB DNA binding activity were quantified by software ImageJ.
T2321 4067-4331 Sentence denotes The DNA-protein complexes were separated by electrophoresis on 4% nondenaturing polyacrylamide gel in 0.5 × TBE buffer (tris-borate-EDTA) at 390 V for 1 hour at 4°C. After electrophoresis, the gel was dried and exposed to X-ray film (Fuji Hyperfilm, Tokyo, Japan).
T2320 3954-4066 Sentence denotes After addition of the 1 μL 32P-labled oligonucleotide probe, the incubation was continued for 20 minutes on ice.
T2319 3747-3953 Sentence denotes Nuclear protein (20 μg) was preincubated in 20 μL binding buffer containing 10 mM Tris-HCl (PH 7.5), 1 mM MgCl2, 0.5 mM NaCl, 4% glycerol, 0.5 mM EDTA, 0.5 mM DTT, and 2 μg poly dI-dC for 20 minutes on ice.
T2318 3589-3746 Sentence denotes Consensus oligonucleotide probe (5′-AGTTGAGGGGACTTTCCCAGGG-3′) was end-labeled by T4-polynucleotide kinase using [γ-32P]-ATP (Free Biotech., Beijing, China).
T2317 3512-3588 Sentence denotes EMSA was performed using gel shift assay system (Promega, Madison, WI, USA).
T2316 3354-3511 Sentence denotes Protein concentration was determined using a bicinchoninic acid assay kit with bovine serum albumin as the standard (Pierce Biochemicals, Rockford, IL, USA).
T2315 3208-3353 Sentence denotes Insoluble material was removed by centrifugation at 14000 rpm, and the supernatant containing the nuclear proteins was stored at –80°C until use.
T2314 3034-3207 Sentence denotes The nuclear proteins were extracted with buffer containing 20 mM HEPES (pH 7.9), 0.4 mM NaCl, 1.0 mM EDTA, 1.0 mM EGTA, 1.0 mM DTT, and 1.0 mM phenylmethylsulfonyl fluoride.
T2313 2932-3033 Sentence denotes Then 10% Nonidet P-40 was added and vortexed briefly, and the nuclei were pelleted by centrifugation.
T2312 2739-2931 Sentence denotes The cell pellet obtained by centrifugation was resuspended in buffer containing 10 mM HEPES (pH 7.9), 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 1.0 mM DTT, and 0.5 mM phenylmethylsulfonyl fluoride.
T2311 2652-2738 Sentence denotes Monolayers of astrocytes were washed with PBS and harvested by scraping into cold PBS.
T2310 2608-2651 Sentence denotes Electrophoretic Mobility Shift Assay (EMSA)
T2309 2603-2607 Sentence denotes 2.4.
T1819 1896-1986 Sentence denotes Cells, which did not received scratch, were used as blank control and named as sham group.
T1818 1764-1895 Sentence denotes Immediately after scratch, cells were washed twice with sterile PBS, cultured with complete culture medium, and named as TBI group.
T1817 1647-1763 Sentence denotes The cell monolayer was scratched with a sterile 26G syringe needle, resulting in the formation of a 0.5 mm wide gap.
T1816 1581-1646 Sentence denotes Astrocytes were planted in 6-well plates and grown to confluence.
T1815 1513-1580 Sentence denotes Astrocyte scratch injury was performed as in a previous report [7].
T1814 1461-1512 Sentence denotes In Vitro Model of TBI Established by Scratch Injury
T1813 1456-1460 Sentence denotes 2.2.
T2041 2543-2601 Sentence denotes Results were expressed as mean of OD value at 450 nm ± SD.
T2040 2494-2542 Sentence denotes All measurements were performed in sextuplicate.
T2039 2388-2493 Sentence denotes Then OD value was read at 450 nm using a Bio-Rad ELISA microplate reader (Bio-Rad Laboratories, CA, USA).
T2038 2318-2387 Sentence denotes Briefly, 10 μL CCK-8 was added into every well and incubated for 1 h.
T2037 2169-2317 Sentence denotes Astrocyte cell death was assessed by cell counting kit-8 (CCK-8) (Dojindo, Japan) assay 24 h after scratch according to the manufacturer's protocol.
T2036 2123-2168 Sentence denotes Then the cell was scrated as mentioned above.
T2035 2031-2122 Sentence denotes 1 × 103/well cells were seeded to 96-well culture plates and cultivated for 24 h to adhere.
T2034 1993-2030 Sentence denotes Cell Death Measurement by CCK-8 Assay
T2033 1988-1992 Sentence denotes 2.3.
T3378 5644-5949 Sentence denotes For immunoblotting, membranes were blocked with 5% nonfat dry milk in saline buffer overnight at 4°C, and the following antibodies were used: anti-β-actin (sc-130657, Santa Cruz Biotechnology, CA, USA, 43 kDa) and anti-matrix metallopeptidase 9 (MMP9) (sc-6841, Santa Cruz Biotechnology, CA, USA, 92 kDa).
T3377 5402-5643 Sentence denotes Fifty micrograms of the resulting cytosolic protein extracts were heat-denatured in Laemmli sample loading buffer, separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis, and electroblotted onto a nitrocellulose membrane.
T3376 5025-5401 Sentence denotes To obtain total protein lysates, cells were homogenized in RIPA buffer (1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, 20 mM NaF, 0.5 mM DTT, 1 mM PMSF, and protease inhibitor cocktail in PBS pH 7.4) and centrifuged at 12,000 g for 15 min at 4°C. Protein concentrations were estimated by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA).
T3375 5012-5024 Sentence denotes Western Blot
T3374 5007-5011 Sentence denotes 2.6.
T1237 1384-1454 Sentence denotes Cells passaged for 2-3 generations were used in the following studies.
T1236 1223-1383 Sentence denotes After shaking, the resulting cultures were mainly astrocytes, which were determined by immunoreactivity for GFAP (sc-166481, Santa Cruz Biotechnology, CA, USA).
T1235 1098-1222 Sentence denotes Then the flasks or dishes were shaken at 150 rpm for 4 h to deplete the microglia and less adherent cells from the cultures.
T1234 1040-1097 Sentence denotes Astrocytes expanded for about 7 days to reach confluence.
T1233 977-1039 Sentence denotes And the complete culture medium was half-changed twice a week.
T1232 925-976 Sentence denotes Then the cells were cultivated at 37°C with 5% CO2.
T1231 785-924 Sentence denotes After incubation for 1 h, the supernatant was transferred to a new flask or dish (Costar, USA) for depleting the residual epithelial cells.
T1230 584-784 Sentence denotes After digestion, the tissue was rinsed twice in DMEM with 10% FBS, followed by a mechanical dissociation in DMEM with 20% FBS and 5 units/mL penicillin, 5 μg/mL streptomycin (complete culture medium).
T1229 449-583 Sentence denotes The tissue was roughly chopped with a scalpel blade, incubated in 0.5% trypsin for 10 minutes at 37°C, and agitated every few minutes.
T1228 332-448 Sentence denotes Following decapitation, the cortices were dissected out, and the meninges and associated blood vessels were removed.
T1227 203-331 Sentence denotes Primary astrocytes were obtained from postnatal 2-day-old Nrf2 wide type (WT) and knockout (KO) mice (6 mice for each genotype).
T1226 86-202 Sentence denotes Nrf2 knockout ICR mice were kindly provided by Dr. Thomas W. Kensler (Johns Hopkins University, Baltimore, MD, USA).
T1225 31-85 Sentence denotes Primary Culture and Identification of Mouse Astrocytes
T1224 26-30 Sentence denotes 2.1.
T26 0-2 Sentence denotes 2.
T27 3-24 Sentence denotes Methods and Materials
T28 26-30 Sentence denotes 2.1.
T29 31-85 Sentence denotes Primary Culture and Identification of Mouse Astrocytes
T30 86-146 Sentence denotes Nrf2 knockout ICR mice were kindly provided by Dr. Thomas W.
T31 147-202 Sentence denotes Kensler (Johns Hopkins University, Baltimore, MD, USA).
T32 203-331 Sentence denotes Primary astrocytes were obtained from postnatal 2-day-old Nrf2 wide type (WT) and knockout (KO) mice (6 mice for each genotype).
T33 332-448 Sentence denotes Following decapitation, the cortices were dissected out, and the meninges and associated blood vessels were removed.
T34 449-583 Sentence denotes The tissue was roughly chopped with a scalpel blade, incubated in 0.5% trypsin for 10 minutes at 37°C, and agitated every few minutes.
T35 584-784 Sentence denotes After digestion, the tissue was rinsed twice in DMEM with 10% FBS, followed by a mechanical dissociation in DMEM with 20% FBS and 5 units/mL penicillin, 5 μg/mL streptomycin (complete culture medium).
T36 785-924 Sentence denotes After incubation for 1 h, the supernatant was transferred to a new flask or dish (Costar, USA) for depleting the residual epithelial cells.
T37 925-976 Sentence denotes Then the cells were cultivated at 37°C with 5% CO2.
T38 977-1039 Sentence denotes And the complete culture medium was half-changed twice a week.
T39 1040-1097 Sentence denotes Astrocytes expanded for about 7 days to reach confluence.
T40 1098-1222 Sentence denotes Then the flasks or dishes were shaken at 150 rpm for 4 h to deplete the microglia and less adherent cells from the cultures.
T41 1223-1383 Sentence denotes After shaking, the resulting cultures were mainly astrocytes, which were determined by immunoreactivity for GFAP (sc-166481, Santa Cruz Biotechnology, CA, USA).
T42 1384-1454 Sentence denotes Cells passaged for 2-3 generations were used in the following studies.
T43 1456-1460 Sentence denotes 2.2.
T44 1461-1512 Sentence denotes In Vitro Model of TBI Established by Scratch Injury
T45 1513-1580 Sentence denotes Astrocyte scratch injury was performed as in a previous report [7].
T46 1581-1646 Sentence denotes Astrocytes were planted in 6-well plates and grown to confluence.
T47 1647-1763 Sentence denotes The cell monolayer was scratched with a sterile 26G syringe needle, resulting in the formation of a 0.5 mm wide gap.
T48 1764-1895 Sentence denotes Immediately after scratch, cells were washed twice with sterile PBS, cultured with complete culture medium, and named as TBI group.
T49 1896-1986 Sentence denotes Cells, which did not received scratch, were used as blank control and named as sham group.
T50 1988-1992 Sentence denotes 2.3.
T51 1993-2030 Sentence denotes Cell Death Measurement by CCK-8 Assay
T52 2031-2122 Sentence denotes 1 × 103/well cells were seeded to 96-well culture plates and cultivated for 24 h to adhere.
T53 2123-2168 Sentence denotes Then the cell was scrated as mentioned above.
T54 2169-2317 Sentence denotes Astrocyte cell death was assessed by cell counting kit-8 (CCK-8) (Dojindo, Japan) assay 24 h after scratch according to the manufacturer's protocol.
T55 2318-2387 Sentence denotes Briefly, 10 μL CCK-8 was added into every well and incubated for 1 h.
T56 2388-2493 Sentence denotes Then OD value was read at 450 nm using a Bio-Rad ELISA microplate reader (Bio-Rad Laboratories, CA, USA).
T57 2494-2542 Sentence denotes All measurements were performed in sextuplicate.
T58 2543-2601 Sentence denotes Results were expressed as mean of OD value at 450 nm ± SD.
T59 2603-2607 Sentence denotes 2.4.
T60 2608-2651 Sentence denotes Electrophoretic Mobility Shift Assay (EMSA)
T61 2652-2738 Sentence denotes Monolayers of astrocytes were washed with PBS and harvested by scraping into cold PBS.
T62 2739-2931 Sentence denotes The cell pellet obtained by centrifugation was resuspended in buffer containing 10 mM HEPES (pH 7.9), 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 1.0 mM DTT, and 0.5 mM phenylmethylsulfonyl fluoride.
T63 2932-3033 Sentence denotes Then 10% Nonidet P-40 was added and vortexed briefly, and the nuclei were pelleted by centrifugation.
T64 3034-3207 Sentence denotes The nuclear proteins were extracted with buffer containing 20 mM HEPES (pH 7.9), 0.4 mM NaCl, 1.0 mM EDTA, 1.0 mM EGTA, 1.0 mM DTT, and 1.0 mM phenylmethylsulfonyl fluoride.
T65 3208-3353 Sentence denotes Insoluble material was removed by centrifugation at 14000 rpm, and the supernatant containing the nuclear proteins was stored at –80°C until use.
T66 3354-3511 Sentence denotes Protein concentration was determined using a bicinchoninic acid assay kit with bovine serum albumin as the standard (Pierce Biochemicals, Rockford, IL, USA).
T67 3512-3588 Sentence denotes EMSA was performed using gel shift assay system (Promega, Madison, WI, USA).
T68 3589-3746 Sentence denotes Consensus oligonucleotide probe (5′-AGTTGAGGGGACTTTCCCAGGG-3′) was end-labeled by T4-polynucleotide kinase using [γ-32P]-ATP (Free Biotech., Beijing, China).
T69 3747-3953 Sentence denotes Nuclear protein (20 μg) was preincubated in 20 μL binding buffer containing 10 mM Tris-HCl (PH 7.5), 1 mM MgCl2, 0.5 mM NaCl, 4% glycerol, 0.5 mM EDTA, 0.5 mM DTT, and 2 μg poly dI-dC for 20 minutes on ice.
T70 3954-4066 Sentence denotes After addition of the 1 μL 32P-labled oligonucleotide probe, the incubation was continued for 20 minutes on ice.
T71 4067-4232 Sentence denotes The DNA-protein complexes were separated by electrophoresis on 4% nondenaturing polyacrylamide gel in 0.5 × TBE buffer (tris-borate-EDTA) at 390 V for 1 hour at 4°C.
T72 4233-4331 Sentence denotes After electrophoresis, the gel was dried and exposed to X-ray film (Fuji Hyperfilm, Tokyo, Japan).
T73 4332-4404 Sentence denotes Levels of NF-κB DNA binding activity were quantified by software ImageJ.
T74 4406-4410 Sentence denotes 2.5.
T75 4411-4417 Sentence denotes RT-PCR
T76 4418-4630 Sentence denotes Total RNA was isolated with Trizol (Invitrogen, CA, USA), and single-stranded cDNA was synthesized from 2 μg of total RNA with BU-Script RT-Kit (Biunique, Jiangsu, China) according to the manufacturer's protocol.
T77 4631-4660 Sentence denotes The cDNA was stored in −20°C.
T78 4661-4785 Sentence denotes Reverse transcription was conducted with GoTaq Green Master Mix (Promega, WI, USA) according to the manufacturer's protocol.
T79 4787-4832 Sentence denotes Table 1 shows the primers and PCR parameters.
T80 4833-4891 Sentence denotes PCR products were detected by agarose gel electrophoresis.
T81 4892-4950 Sentence denotes The intensity of the bands was analyzed by ImageJ program.
T82 4951-5005 Sentence denotes The level of β-actin was used as an internal standard.
T83 5007-5011 Sentence denotes 2.6.
T84 5012-5024 Sentence denotes Western Blot
T85 5025-5304 Sentence denotes To obtain total protein lysates, cells were homogenized in RIPA buffer (1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, 20 mM NaF, 0.5 mM DTT, 1 mM PMSF, and protease inhibitor cocktail in PBS pH 7.4) and centrifuged at 12,000 g for 15 min at 4°C.
T86 5305-5401 Sentence denotes Protein concentrations were estimated by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA).
T87 5402-5643 Sentence denotes Fifty micrograms of the resulting cytosolic protein extracts were heat-denatured in Laemmli sample loading buffer, separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis, and electroblotted onto a nitrocellulose membrane.
T88 5644-5949 Sentence denotes For immunoblotting, membranes were blocked with 5% nonfat dry milk in saline buffer overnight at 4°C, and the following antibodies were used: anti-β-actin (sc-130657, Santa Cruz Biotechnology, CA, USA, 43 kDa) and anti-matrix metallopeptidase 9 (MMP9) (sc-6841, Santa Cruz Biotechnology, CA, USA, 92 kDa).
T89 5950-6073 Sentence denotes Each primary antibody was diluted appropriately in blocking buffer and then added to the blots for 1 h at room temperature.
T90 6074-6231 Sentence denotes The blots were washed three times in the washing buffer and covered with the horseradish peroxidase-linked secondary antibody at a 1 : 2000 dilution for 1 h.
T91 6232-6406 Sentence denotes Blots were incubated with enhanced chemiluminescence (ECL) detection system (Amersham Biosciences, Bucks, UK) and exposed to radiographic film (Fuji Hyperfilm, Tokyo, Japan).
T92 6407-6461 Sentence denotes ImageJ was used to analyze the intensity of the blots.
T93 6462-6513 Sentence denotes The level of β-actin was used as internal standard.
T94 6515-6519 Sentence denotes 2.7.
T95 6520-6561 Sentence denotes Enzyme-Linked Immunosorbent Assay (ELISA)
T96 6562-6619 Sentence denotes Cells of four groups were homogenized as mentioned above.
T97 6620-6742 Sentence denotes The supernatant was collected, and total protein was determined by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA).
T98 6743-6971 Sentence denotes Levels of tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6) protein were quantified using ELISA kits specific for mouse according to the manufacturer's instructions (Bender MedSystems Inc.
T99 6972-6981 Sentence denotes CA, USA).
T100 6982-7075 Sentence denotes Briefly, prepared the standard and created the standard dilution for building standard curve.
T101 7076-7140 Sentence denotes Then samples and biotinconjugate were added to microwell strips.
T102 7141-7290 Sentence denotes After incubated for 2 h at room temperature, the microwell strips were washed 3 times with wash buffer, and streptavidin-HRP were added to all wells.
T103 7291-7386 Sentence denotes After incubated for 1 h, microwell strips were washed 3 times followed by adding TMB substrate.
T104 7387-7452 Sentence denotes After incubated for about 10–30 min, the stop solution was added.
T105 7453-7569 Sentence denotes The colour intensity was measured at 450 nm using a Bio-Rad ELISA microplate reader (Bio-Rad Laboratories, CA, USA).
T106 7570-7686 Sentence denotes The concentration of protein was determined according to the standard curve and expressed as pg/mg of total protein.
T107 7688-7692 Sentence denotes 2.8.
T108 7693-7712 Sentence denotes Gelatine Zymography
T109 7713-7858 Sentence denotes Cells of four groups were homogenized in lysis buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 5 mM CaCl2, 0.2 mM NaN3, and 0.01% Triton.
T110 7859-7929 Sentence denotes Soluble extracts were separated by centrifugation and stored at –20°C.
T111 7930-8042 Sentence denotes Gelatin zymography was performed according to the manufacturer's instructions (Genmed Scientifics Inc, MA, USA).
T112 8043-8119 Sentence denotes Briefly, 40 μg cytosolic protein extracts were separated by electrophoresis.
T113 8120-8285 Sentence denotes Then the proteins were renatured by incubation in 2.5% Triton X-100 and then incubated in substrate buffer for 40 h at 37°C to enable the MMP9 to cleave the gelatin.
T114 8286-8389 Sentence denotes After rinsing in water, each gel was stained with Coomassie blue for 1 h and destained in 50% methanol.
T115 8390-8495 Sentence denotes Proteolytic activities were showed by clear bands in blue gel which indicates the lysis of the substrate.
T116 8496-8581 Sentence denotes Quantification of MMP9 band density was performed with image analysis program ImageJ.
T117 8583-8587 Sentence denotes 2.9.
T118 8588-8608 Sentence denotes Statistical Analysis
T119 8609-8698 Sentence denotes Data were expressed as mean ± SD and evaluated by ANOVA and LSD multiple comparison test.
T120 8699-8748 Sentence denotes P values <0.05 were considered to be significant.
T121 8749-8805 Sentence denotes All analyses were performed by using SPSS 18.0 software.

ICD10

Id Subject Object Predicate Lexical cue
T1918 1531-1537 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T1917 1506-1512 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes Injury

simple1

Id Subject Object Predicate Lexical cue
T4551 8514-8518 Protein denotes MMP9
T4550 8258-8262 Protein denotes MMP9
T3225 4964-4971 Protein denotes β-actin
T4288 6837-6841 Protein denotes IL-6
T4287 6822-6835 Protein denotes interleukin-6
T4286 6810-6815 Protein denotes IL-1β
T4285 6790-6808 Protein denotes interleukin-1 beta
T3686 5890-5894 Protein denotes MMP9

BioNLP16_DUT

Id Subject Object Predicate Lexical cue
T4732 8514-8518 Protein denotes MMP9
T4731 8258-8262 Protein denotes MMP9
T4337 6837-6841 Protein denotes IL-6
T4336 6822-6835 Protein denotes interleukin-6
T4335 6810-6815 Protein denotes IL-1β
T4334 6790-6808 Protein denotes interleukin-1 beta
T3997 5890-5894 Protein denotes MMP9
T3353 4964-4971 Protein denotes β-actin

BioNLP16_Messiy

Id Subject Object Predicate Lexical cue
T4299 6837-6841 Protein denotes IL-6
T4298 6822-6835 Protein denotes interleukin-6
T4297 6810-6815 Protein denotes IL-1β
T4296 6790-6808 Protein denotes interleukin-1 beta
T4556 8266-8272 Protein_catabolism denotes cleave
T4555 8514-8518 Protein denotes MMP9
T4554 8258-8262 Protein denotes MMP9
T3690 5890-5894 Protein denotes MMP9
T3228 4964-4971 Protein denotes β-actin
R3872 T4554 T4556 themeOf MMP9,cleave

DLUT931

Id Subject Object Predicate Lexical cue
T4295 6837-6841 Protein denotes IL-6
T4294 6822-6835 Protein denotes interleukin-6
T4293 6810-6815 Protein denotes IL-1β
T4292 6790-6808 Protein denotes interleukin-1 beta
T4553 8514-8518 Protein denotes MMP9
T4552 8258-8262 Protein denotes MMP9
T3689 5890-5894 Protein denotes MMP9
T3227 4964-4971 Protein denotes β-actin

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T4842 8669-8672 Protein denotes LSD
T4841 8632-8641 Entity denotes mean ± SD
T4840 8659-8664 Protein denotes ANOVA
T4752 7693-7701 Entity denotes Gelatine
T4751 8210-8219 Entity denotes substrate
T4750 8052-8084 Entity denotes 40 μg cytosolic protein extracts
T4749 7713-7733 Entity denotes Cells of four groups
T4748 8355-8358 Entity denotes 1 h
T4747 8170-8187 Entity denotes 2.5% Triton X-100
T4746 7859-7875 Entity denotes Soluble extracts
T4745 8254-8262 Protein denotes the MMP9
T4744 7828-7857 Entity denotes 0.2 mM NaN3, and 0.01% Triton
T4743 7845-7857 Entity denotes 0.01% Triton
T4742 7778-7792 Entity denotes 50 mM Tris-HCl
T4741 8443-8494 Entity denotes blue gel which indicates the lysis of the substrate
T4740 7803-7814 Entity denotes 150 mM NaCl
T4739 8310-8318 Entity denotes each gel
T4738 8514-8518 Protein denotes MMP9
T4737 8052-8084 Protein denotes 40 μg cytosolic protein extracts
T4736 7930-7937 Entity denotes Gelatin
T4735 8125-8137 Protein denotes the proteins
T4734 7778-7792 Entity denotes 50 mM Tris-HCl
T4733 8376-8388 Entity denotes 50% methanol
T4361 6843-6850 Protein denotes protein
T4360 7161-7185 Entity denotes 2 h at room temperature,
T4359 6810-6815 Protein denotes IL-1β
T4358 7249-7265 Protein denotes streptavidin-HRP
T4357 6702-6723 Entity denotes Protein Assay Reagent
T4356 6837-6841 Protein denotes IL-6
T4355 6782-6787 Protein denotes TNF-α
T4354 7249-7265 Protein denotes streptavidin-HRP
T4353 6620-6635 Entity denotes The supernatant
T4352 7262-7265 Protein denotes HRP
T4351 7588-7598 Protein denotes of protein
T4350 6822-6835 Protein denotes interleukin-6
T4349 6655-6668 Protein denotes total protein
T4348 7311-7315 Entity denotes 1 h,
T4347 6733-6735 Entity denotes IL
T4346 6750-6788 Protein denotes of tumor necrosis factor-alpha (TNF-α)
T4345 6562-6618 Entity denotes Cells of four groups were homogenized as mentioned above
T4344 6702-6723 Entity denotes Protein Assay Reagent
T4343 7372-7385 Entity denotes TMB substrate
T4342 7424-7441 Entity denotes the stop solution
T4341 6702-6723 Entity denotes Protein Assay Reagent
T4340 6949-6971 Entity denotes Bender MedSystems Inc.
T4339 7672-7685 Protein denotes total protein
T4338 6790-6817 Protein denotes interleukin-1 beta (IL-1β),
T4033 6331-6340 Protein denotes Bucks, UK
T4032 5384-5394 Entity denotes Pierce, IL
T4031 5302-5312 Protein denotes C. Protein
T4030 6286-6289 Protein denotes ECL
T4029 6475-6482 Protein denotes β-actin
T4028 5454-5462 Entity denotes extracts
T4027 5714-5720 Entity denotes saline
T4026 5619-5642 Entity denotes nitrocellulose membrane
T4025 5177-5186 Entity denotes 20 mM NaF
T4024 5750-5774 Entity denotes the following antibodies
T4023 5142-5163 Entity denotes 1 mM EDTA, 1 mM EGTA,
T4022 5200-5209 Entity denotes 1 mM PMSF
T4021 5890-5894 Protein denotes MMP9
T4020 5858-5895 Protein denotes anti-matrix metallopeptidase 9 (MMP9)
T4019 5619-5633 Entity denotes nitrocellulose
T4018 5215-5223 Protein denotes protease
T4017 5446-5453 Protein denotes protein
T4016 5527-5591 Entity denotes by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis
T4015 5955-5971 Protein denotes primary antibody
T4014 5058-5063 Entity denotes cells
T4013 5035-5057 Protein denotes total protein lysates,
T4012 5132-5140 Protein denotes 0.1% SDS
T4011 6181-6199 Protein denotes secondary antibody
T4010 5375-5395 Entity denotes Reagent (Pierce, IL,
T4009 5527-5591 Entity denotes by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis
T4008 5106-5141 Entity denotes 0.5% sodium deoxycholate, 0.1% SDS,
T4007 5780-5810 Protein denotes used: anti-β-actin (sc-130657,
T4006 5664-5673 Entity denotes membranes
T4005 5361-5368 Entity denotes Protein
T4004 6134-6173 Protein denotes covered with the horseradish peroxidase
T4003 5557-5571 Entity denotes polyacrylamide
T4002 5215-5242 Entity denotes protease inhibitor cocktail
T4001 5906-5911 Entity denotes Santa
T4000 5811-5835 Entity denotes Santa Cruz Biotechnology
T3999 5153-5162 Entity denotes 1 mM EGTA
T3998 5188-5198 Entity denotes 0.5 mM DTT
T3095 3034-3104 Negative_regulation denotes The nuclear proteins were extracted with buffer containing 20 mM HEPES
T3094 2878-2889 Entity denotes 1.0 mM DTT,
T3093 2819-2830 Entity denotes 10 mM HEPES
T3092 3399-3417 Entity denotes bicinchoninic acid
T3091 2859-2864 Entity denotes EDTA,
T3090 3492-3510 Entity denotes Rockford, IL, USA)
T3089 3823-3837 Entity denotes 10 mM Tris-HCl
T3088 3920-3924 Entity denotes poly
T3087 3899-3909 Entity denotes 0.5 mM DTT
T3086 3354-3379 Entity denotes Protein concentration was
T3085 4067-4092 Entity denotes The DNA-protein complexes
T3084 3860-3885 Entity denotes 0.5 mM NaCl, 4% glycerol,
T3083 2847-2851 Entity denotes KCl,
T3082 3433-3453 Protein denotes bovine serum albumin
T3081 2990-3000 Entity denotes the nuclei
T3080 2832-2834 Protein denotes pH
T3079 4130-4165 Entity denotes 4% nondenaturing polyacrylamide gel
T3078 3886-3910 Entity denotes 0.5 mM EDTA, 0.5 mM DTT,
T3077 4256-4263 Entity denotes the gel
T3076 2894-2930 Entity denotes 0.5 mM phenylmethylsulfonyl fluoride
T3075 3141-3165 Entity denotes 1.0 mM EGTA, 1.0 mM DTT,
T3074 3671-3695 Entity denotes T4-polynucleotide kinase
T3062 3302-3322 Protein denotes the nuclear proteins
T3061 3471-3490 Entity denotes Pierce Biochemicals
T3060 2968-2976 Entity denotes vortexed
T3059 3034-3054 Protein denotes The nuclear proteins
T3058 3674-3695 Protein denotes polynucleotide kinase
T3057 3397-3427 Entity denotes a bicinchoninic acid assay kit
T3056 3671-3695 Entity denotes T4-polynucleotide kinase
T3055 3709-3729 Entity denotes -ATP (Free Biotech.,
T3054 2937-2953 Entity denotes 10% Nonidet P-40
T3053 3935-3952 Entity denotes 20 minutes on ice
T3052 4342-4347 Protein denotes NF-κB
T3051 4187-4191 Entity denotes tris
T3050 3537-3540 Entity denotes gel
T3049 4147-4161 Entity denotes polyacrylamide
T3048 4187-4203 Entity denotes tris-borate-EDTA
T3047 4192-4198 Entity denotes borate
T3046 3823-3837 Entity denotes 10 mM Tris-HCl
T3045 4048-4065 Entity denotes 20 minutes on ice
T3044 2739-2785 Entity denotes The cell pellet obtained by centrifugation was
T3043 2841-2876 Entity denotes 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA
T3042 3128-3139 Entity denotes 1.0 mM EDTA
T3041 3154-3164 Entity denotes 1.0 mM DTT
T3040 3093-3104 Entity denotes 20 mM HEPES
T3039 2666-2676 Entity denotes astrocytes
T3366 4418-4427 Protein denotes Total RNA
T3365 4833-4836 Protein denotes PCR
T3364 4480-4500 Entity denotes single-stranded cDNA
T3363 4631-4639 Entity denotes The cDNA
T3362 4527-4539 Protein denotes of total RNA
T3361 4446-4465 Entity denotes Trizol (Invitrogen,
T3360 4545-4588 Protein denotes BU-Script RT-Kit (Biunique, Jiangsu, China)
T3359 4522-4539 Protein denotes 2 μg of total RNA
T3358 4411-4417 Protein denotes RT-PCR
T3357 4863-4890 Entity denotes agarose gel electrophoresis
T3356 4863-4870 Entity denotes agarose
T3355 4817-4820 Protein denotes PCR
T3354 4964-4971 Protein denotes β-actin
T3073 3839-3841 Protein denotes PH
T3072 3275-3322 Entity denotes the supernatant containing the nuclear proteins
T3071 3873-3884 Entity denotes 4% glycerol
T3070 3170-3206 Entity denotes 1.0 mM phenylmethylsulfonyl fluoride
T3069 4175-4204 Entity denotes TBE buffer (tris-borate-EDTA)
T3068 3106-3108 Protein denotes pH
T3067 3747-3762 Protein denotes Nuclear protein
T3066 4187-4203 Entity denotes tris-borate-EDTA
T3065 3589-3651 Entity denotes Consensus oligonucleotide probe (5′-AGTTGAGGGGACTTTCCCAGGG-3′)
T3064 3969-4013 Entity denotes of the 1 μL 32P-labled oligonucleotide probe
T3063 3115-3140 Entity denotes 0.4 mM NaCl, 1.0 mM EDTA,
T2307 2333-2338 Entity denotes CCK-8
T2306 2227-2232 Entity denotes CCK-8
T2305 2383-2386 Entity denotes 1 h
T2304 2110-2121 Entity denotes h to adhere
T2303 2169-2178 Entity denotes Astrocyte
T2302 2206-2210 Entity denotes cell
T2301 2257-2275 Entity denotes 24 h after scratch
T2300 2019-2024 Entity denotes CCK-8
T2299 2039-2049 Entity denotes well cells
T2298 2128-2136 Entity denotes the cell
T2297 1993-1997 Entity denotes Cell
T1812 203-298 Binding denotes Primary astrocytes were obtained from postnatal 2-day-old Nrf2 wide type (WT) and knockout (KO)
T1811 1040-1050 Entity denotes Astrocytes
T1810 299-330 Protein denotes mice (6 mice for each genotype)
T1809 203-221 Entity denotes Primary astrocytes
T1808 66-85 Entity denotes of Mouse Astrocytes
T1807 305-329 Protein denotes 6 mice for each genotype
T1806 261-265 Protein denotes Nrf2
T1805 1166-1179 Entity denotes the microglia
T1804 1151-1154 Entity denotes 4 h
T1803 894-923 Entity denotes the residual epithelial cells
T1802 642-650 Protein denotes 10% FBS,
T1801 86-90 Protein denotes Nrf2
T1800 1331-1347 Protein denotes GFAP (sc-166481,
T1799 737-783 Entity denotes 5 μg/mL streptomycin (complete culture medium)
T1798 716-735 Entity denotes units/mL penicillin
T1797 1348-1373 Protein denotes Santa Cruz Biotechnology,
T1796 1384-1389 Entity denotes Cells
T1795 1184-1203 Entity denotes less adherent cells
T1794 702-709 Protein denotes 20% FBS
T1793 1273-1284 Entity denotes astrocytes,
T1792 811-826 Entity denotes the supernatant
T1791 295-297 Protein denotes KO
T1790 515-550 Entity denotes 0.5% trypsin for 10 minutes at 37°C
T1789 806-809 Entity denotes 1 h
T2031 1820-1870 Regulation denotes sterile PBS, cultured with complete culture medium
T2030 1791-1796 Entity denotes cells
T2029 1647-1655 Entity denotes The cell
T2028 1513-1579 Entity denotes Astrocyte scratch injury was performed as in a previous report [7]
T2027 1581-1591 Entity denotes Astrocytes
T2026 1820-1870 Protein denotes sterile PBS, cultured with complete culture medium
T2025 1896-1916 Entity denotes Cells, which did not
T2024 1975-1979 Entity denotes sham
T2023 1687-1694 Protein denotes sterile
R1725 T2026 T2031 themeOf "sterile PBS, cultured with complete culture medium","sterile PBS, cultured with complete culture medium"
R2676 T3059 T3095 themeOf The nuclear proteins,The nuclear proteins were extracted with buffer containing 20 mM HEPES

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T4839 8804-8805 . denotes .
T4838 8796-8804 NN denotes software
T4837 8791-8795 CD denotes 18.0
T4836 8786-8790 NNP denotes SPSS
T4835 8780-8785 VBG denotes using
T4834 8777-8779 IN denotes by
T4833 8767-8776 VBN denotes performed
T4832 8762-8766 VBD denotes were
T4831 8753-8761 NNS denotes analyses
T4830 8749-8752 DT denotes All
T4829 8747-8748 . denotes .
T4828 8736-8747 JJ denotes significant
T4827 8733-8735 VB denotes be
T4826 8730-8732 TO denotes to
T4825 8719-8729 VBN denotes considered
T4824 8714-8718 VBD denotes were
T4823 8709-8713 CD denotes 0.05
T4822 8708-8709 VBP denotes <
T4821 8701-8707 NNS denotes values
T4820 8699-8700 NN denotes P
T4819 8697-8698 . denotes .
T4818 8693-8697 NN denotes test
T4817 8682-8692 NN denotes comparison
T4816 8673-8681 JJ denotes multiple
T4815 8669-8672 NNP denotes LSD
T4814 8665-8668 CC denotes and
T4813 8659-8664 NNP denotes ANOVA
T4812 8656-8658 IN denotes by
T4811 8646-8655 VBN denotes evaluated
T4810 8642-8645 CC denotes and
T4809 8639-8641 NNP denotes SD
T4808 8637-8638 NN denotes ±
T4807 8632-8636 JJ denotes mean
T4806 8629-8631 IN denotes as
T4805 8619-8628 VBN denotes expressed
T4804 8614-8618 VBD denotes were
T4803 8609-8613 NNP denotes Data
T4802 8600-8608 NNP denotes Analysis
T4801 8588-8599 NNP denotes Statistical
T4800 8586-8587 . denotes .
T4799 8583-8586 CD denotes 2.9
T4729 8580-8581 . denotes .
T4728 8574-8580 NNP denotes ImageJ
T4727 8566-8573 NN denotes program
T4726 8557-8565 NN denotes analysis
T4725 8551-8556 NN denotes image
T4723 8536-8545 VBN denotes performed
T4722 8532-8535 VBD denotes was
T4721 8524-8531 NN denotes density
T4720 8519-8523 NN denotes band
T4719 8514-8518 NNP denotes MMP9
T4718 8511-8513 IN denotes of
T4717 8496-8510 NN denotes Quantification
T4716 8494-8495 . denotes .
T4715 8485-8494 NN denotes substrate
T4714 8481-8484 DT denotes the
T4713 8478-8480 IN denotes of
T4712 8472-8477 NN denotes lysis
T4711 8468-8471 DT denotes the
T4710 8458-8467 VBZ denotes indicates
T4709 8452-8457 WDT denotes which
T4708 8448-8451 NN denotes gel
T4707 8443-8447 JJ denotes blue
T4706 8440-8442 IN denotes in
T4705 8434-8439 NNS denotes bands
T4704 8428-8433 JJ denotes clear
T4703 8425-8427 IN denotes by
T4702 8418-8424 VBN denotes showed
T4701 8413-8417 VBD denotes were
T4700 8402-8412 NNS denotes activities
T4699 8390-8401 JJ denotes Proteolytic
T4698 8388-8389 . denotes .
T4697 8380-8388 NN denotes methanol
T4696 8378-8379 NN denotes %
T4695 8376-8378 CD denotes 50
T4694 8373-8375 IN denotes in
T4693 8363-8372 VBN denotes destained
T4692 8359-8362 CC denotes and
T4686 8331-8335 IN denotes with
T4685 8323-8330 VBN denotes stained
T4684 8319-8322 VBD denotes was
T4683 8315-8318 NN denotes gel
T4682 8310-8314 DT denotes each
T4652 8173-8174 NN denotes %
T4651 8170-8173 CD denotes 2.5
T4650 8167-8169 IN denotes in
T4649 8156-8166 NN denotes incubation
T4648 8153-8155 IN denotes by
T4647 8143-8152 VBN denotes renatured
T4646 8138-8142 VBD denotes were
T4645 8129-8137 NNS denotes proteins
T4644 8125-8128 DT denotes the
T4643 8120-8124 RB denotes Then
T4642 8118-8119 . denotes .
T4641 8103-8118 NN denotes electrophoresis
T4640 8100-8102 IN denotes by
T4639 8090-8099 VBN denotes separated
T4638 8085-8089 VBD denotes were
T4637 8076-8084 NNS denotes extracts
T4636 8068-8075 NN denotes protein
T4635 8058-8067 JJ denotes cytosolic
T4634 8055-8057 NN denotes μg
T4633 8052-8054 CD denotes 40
T4632 8050-8051 , denotes ,
T4631 8043-8050 RB denotes Briefly
T4630 8041-8042 . denotes .
T4629 8040-8041 -RRB- denotes )
T4628 8037-8040 NNP denotes USA
T4627 8035-8036 , denotes ,
T4626 8033-8035 NNP denotes MA
T4625 8031-8032 , denotes ,
T4624 8028-8031 NNP denotes Inc
T4623 8016-8027 NNP denotes Scientifics
T4622 8009-8015 NNP denotes Genmed
T4621 8008-8009 -LRB- denotes (
T4620 7995-8007 NNS denotes instructions
T4619 7992-7994 POS denotes 's
T4618 7980-7992 NN denotes manufacturer
T4617 7976-7979 DT denotes the
T4616 7973-7975 TO denotes to
T4615 7963-7972 VBG denotes according
T4614 7953-7962 VBN denotes performed
T4613 7949-7952 VBD denotes was
T4612 7938-7948 NN denotes zymography
T4611 7930-7937 NNP denotes Gelatin
T4610 7927-7929 NNP denotes C.
T4609 7926-7927 NN denotes °
T4608 7924-7926 CD denotes 20
T4607 7920-7922 IN denotes at
T4606 7913-7919 VBD denotes stored
T4605 7909-7912 CC denotes and
T4604 7894-7908 NN denotes centrifugation
T4603 7891-7893 IN denotes by
T4602 7881-7890 VBN denotes separated
T4601 7876-7880 VBD denotes were
T4600 7867-7875 NNS denotes extracts
T4599 7859-7866 JJ denotes Soluble
T4598 7857-7858 . denotes .
T4597 7851-7857 NNP denotes Triton
T4596 7849-7850 NN denotes %
T4595 7845-7849 CD denotes 0.01
T4594 7841-7844 CC denotes and
T4593 7839-7840 , denotes ,
T4592 7835-7839 NNP denotes NaN3
T4591 7832-7834 NNP denotes mM
T4590 7828-7831 CD denotes 0.2
T4589 7826-7827 , denotes ,
T4588 7821-7826 NNP denotes CaCl2
T4587 7818-7820 NNP denotes mM
T4586 7816-7817 CD denotes 5
T4585 7814-7815 , denotes ,
T4584 7810-7814 NNP denotes NaCl
T4583 7807-7809 NNP denotes mM
T4582 7803-7806 CD denotes 150
T4581 7801-7802 , denotes ,
T4580 7800-7801 -RRB- denotes )
T4579 7797-7800 CD denotes 7.4
T4578 7794-7796 NNP denotes pH
T4577 7793-7794 -LRB- denotes (
T4576 7784-7792 NNP denotes Tris-HCl
T4575 7781-7783 NNP denotes mM
T4574 7778-7780 CD denotes 50
T4573 7767-7777 VBG denotes containing
T4572 7760-7766 NN denotes buffer
T4571 7754-7759 NN denotes lysis
T4570 7751-7753 IN denotes in
T4569 7739-7750 VBN denotes homogenized
T4568 7734-7738 VBD denotes were
T4567 7727-7733 NNS denotes groups
T4566 7722-7726 CD denotes four
T4565 7719-7721 IN denotes of
T4564 7713-7718 NNP denotes Cells
T4563 7702-7712 NNP denotes Zymography
T4562 7693-7701 NNP denotes Gelatine
T4561 7691-7692 . denotes .
T4560 7688-7691 CD denotes 2.8
T3991 6512-6513 . denotes .
T3990 6504-6512 NN denotes standard
T3989 6495-6503 JJ denotes internal
T3988 6492-6494 IN denotes as
T3987 6487-6491 VBN denotes used
T3986 6483-6486 VBD denotes was
T3985 6475-6482 NN denotes β-actin
T3984 6472-6474 IN denotes of
T3983 6466-6471 NN denotes level
T3982 6462-6465 DT denotes The
T3981 6460-6461 . denotes .
T3980 6455-6460 NNS denotes blots
T3979 6451-6454 DT denotes the
T3978 6448-6450 IN denotes of
T3977 6438-6447 NN denotes intensity
T3976 6434-6437 DT denotes the
T3975 6426-6433 VB denotes analyze
T3974 6423-6425 TO denotes to
T3973 6418-6422 VBN denotes used
T3972 6414-6417 VBD denotes was
T3971 6407-6413 NNP denotes ImageJ
T3970 6405-6406 . denotes .
T3969 6404-6405 -RRB- denotes )
T3704 5041-5048 NN denotes protein
T3703 5035-5040 JJ denotes total
T3702 5028-5034 VB denotes obtain
T3701 5025-5027 TO denotes To
T3700 5020-5024 NNP denotes Blot
T3699 5012-5019 JJ denotes Western
T3698 5010-5011 . denotes .
T3697 5007-5010 CD denotes 2.6
T4691 8357-8358 NN denotes h
T4690 8355-8356 CD denotes 1
T4689 8351-8354 IN denotes for
T4688 8346-8350 JJ denotes blue
T4687 8336-8345 NNP denotes Coomassie
T3289 4683-4686 VBD denotes was
T3288 4669-4682 NN denotes transcription
T3287 4661-4668 VB denotes Reverse
T3286 4658-4660 NNP denotes C.
T3285 4657-4658 NN denotes °
T3284 4655-4657 CD denotes 20
T3283 4654-4655 CD denotes −
T3282 4651-4653 IN denotes in
T3281 4644-4650 VBN denotes stored
T3280 4640-4643 VBD denotes was
T3279 4635-4639 NNP denotes cDNA
T3278 4631-4634 DT denotes The
T3277 4629-4630 . denotes .
T3276 4621-4629 NN denotes protocol
T3275 4618-4620 POS denotes 's
T3274 4606-4618 NN denotes manufacturer
T3273 4602-4605 DT denotes the
T3272 4599-4601 TO denotes to
T3271 4589-4598 VBG denotes according
T3270 4587-4588 -RRB- denotes )
T3269 4582-4587 NNP denotes China
T3268 4580-4581 , denotes ,
T3267 4573-4580 NNP denotes Jiangsu
T3266 4571-4572 , denotes ,
T3265 4563-4571 NNP denotes Biunique
T3264 4562-4563 -LRB- denotes (
T3263 4555-4561 NN denotes RT-Kit
T3262 4545-4554 JJ denotes BU-Script
T3261 4540-4544 IN denotes with
T3260 4536-4539 NNP denotes RNA
T3259 4530-4535 JJ denotes total
T3258 4527-4529 IN denotes of
T3257 4524-4526 NN denotes μg
T3256 4522-4523 CD denotes 2
T3255 4517-4521 IN denotes from
T3254 4505-4516 VBN denotes synthesized
T3253 4501-4504 VBD denotes was
T3252 4496-4500 NNP denotes cDNA
T3251 4480-4495 JJ denotes single-stranded
T3250 4476-4479 CC denotes and
T3249 4474-4475 , denotes ,
T3248 4473-4474 -RRB- denotes )
T3247 4470-4473 NNP denotes USA
T3246 4468-4469 , denotes ,
T3245 4466-4468 NNP denotes CA
T3244 4464-4465 , denotes ,
T3243 4454-4464 NNP denotes Invitrogen
T3242 4453-4454 -LRB- denotes (
T3241 4446-4452 NNP denotes Trizol
T3240 4441-4445 IN denotes with
T3239 4432-4440 VBN denotes isolated
T3238 4428-4431 VBD denotes was
T3237 4424-4427 NNP denotes RNA
T3236 4418-4423 NNP denotes Total
T3235 4411-4417 NNP denotes RT-PCR
T3234 4409-4410 . denotes .
T3233 4406-4409 CD denotes 2.5
T3894 5984-5997 RB denotes appropriately
T3893 5976-5983 VBN denotes diluted
T3892 5972-5975 VBD denotes was
T3891 5963-5971 NN denotes antibody
T3865 5849-5852 NN denotes kDa
T3864 5846-5848 CD denotes 43
T3863 5844-5845 , denotes ,
T3862 5841-5844 NNP denotes USA
T3861 5839-5840 , denotes ,
T3860 5837-5839 NNP denotes CA
T3859 5835-5836 , denotes ,
T3858 5822-5835 NNP denotes Biotechnology
T3857 5817-5821 NNP denotes Cruz
T3856 5811-5816 NNP denotes Santa
T3855 5809-5810 , denotes ,
T3854 5800-5809 JJ denotes sc-130657
T3853 5799-5800 -LRB- denotes (
T3852 5786-5798 JJ denotes anti-β-actin
T3851 5784-5785 : denotes :
T3850 5780-5784 VBN denotes used
T3849 5775-5779 VBD denotes were
T3848 5764-5774 NNS denotes antibodies
T3847 5754-5763 VBG denotes following
T3846 5750-5753 DT denotes the
T3845 5746-5749 CC denotes and
T3844 5744-5745 , denotes ,
T3843 5743-5744 NNP denotes C
T3842 5742-5743 CD denotes °
T3841 5741-5742 CD denotes 4
T3840 5738-5740 IN denotes at
T3839 5728-5737 JJ denotes overnight
T3838 5721-5727 NN denotes buffer
T3837 5714-5720 NN denotes saline
T3836 5711-5713 IN denotes in
T3835 5706-5710 NN denotes milk
T3834 5702-5705 JJ denotes dry
T3833 5695-5701 JJ denotes nonfat
T3832 5693-5694 NN denotes %
T3831 5692-5693 CD denotes 5
T3830 5687-5691 IN denotes with
T3829 5679-5686 VBN denotes blocked
T3828 5674-5678 VBD denotes were
T3827 5664-5673 NNS denotes membranes
T3826 5662-5663 , denotes ,
T3825 5648-5662 VBG denotes immunoblotting
T3824 5644-5647 IN denotes For
T3823 5642-5643 . denotes .
T3822 5634-5642 NN denotes membrane
T3821 5619-5633 JJ denotes nitrocellulose
T3820 5617-5618 DT denotes a
T3819 5612-5616 IN denotes onto
T3818 5597-5611 VBD denotes electroblotted
T3817 5593-5596 CC denotes and
T3816 5591-5592 , denotes ,
T3815 5576-5591 NN denotes electrophoresis
T3814 5572-5575 NN denotes gel
T3813 5557-5571 NN denotes polyacrylamide
T3812 5549-5556 NN denotes sulfate
T3811 5541-5548 NN denotes dodecyl
T3810 5534-5540 NN denotes sodium
T3809 5532-5533 NN denotes %
T3808 5530-5532 CD denotes 10
T3787 5396-5399 NNP denotes USA
T3786 5394-5395 , denotes ,
T3785 5392-5394 NNP denotes IL
T3784 5390-5391 , denotes ,
T3783 5384-5390 NNP denotes Pierce
T3782 5383-5384 -LRB- denotes (
T3781 5375-5382 NNP denotes Reagent
T3780 5369-5374 NNP denotes Assay
T3779 5361-5368 NNP denotes Protein
T3778 5356-5360 NNP denotes Plus
T3777 5346-5355 NNP denotes Coomassie
T3776 5343-5345 IN denotes by
T3775 5333-5342 VBN denotes estimated
T3774 5328-5332 VBD denotes were
T3773 5313-5327 NNS denotes concentrations
T3772 5305-5312 NNP denotes Protein
T3771 5302-5304 NNP denotes C.
T3770 5301-5302 CD denotes °
T3769 5300-5301 CD denotes 4
T3768 5297-5299 IN denotes at
T3767 5293-5296 NN denotes min
T3766 5290-5292 CD denotes 15
T3765 5286-5289 IN denotes for
T3764 5284-5285 NN denotes g
T3763 5277-5283 CD denotes 12,000
T3762 5274-5276 IN denotes at
T3761 5262-5273 VBN denotes centrifuged
T3760 5258-5261 CC denotes and
T3759 5256-5257 -RRB- denotes )
T3758 5253-5256 CD denotes 7.4
T3757 5250-5252 NNP denotes pH
T3756 5246-5249 NNP denotes PBS
T3755 5243-5245 IN denotes in
T3754 5234-5242 NN denotes cocktail
T3753 5224-5233 NN denotes inhibitor
T3752 5215-5223 NN denotes protease
T3751 5211-5214 CC denotes and
T3750 5209-5210 , denotes ,
T3749 5205-5209 NNP denotes PMSF
T3748 5202-5204 NNP denotes mM
T3747 5200-5201 CD denotes 1
T3746 5198-5199 , denotes ,
T3745 5195-5198 NNP denotes DTT
T3744 5192-5194 NNP denotes mM
T3743 5188-5191 CD denotes 0.5
T3742 5186-5187 , denotes ,
T3741 5183-5186 NNP denotes NaF
T3740 5180-5182 NNP denotes mM
T3739 5177-5179 CD denotes 20
T3738 5175-5176 , denotes ,
T3737 5169-5175 NNP denotes Na3VO4
T3736 5166-5168 NNP denotes mM
T3735 5164-5165 CD denotes 1
T3734 5162-5163 , denotes ,
T3733 5158-5162 NNP denotes EGTA
T3732 5155-5157 NNP denotes mM
T3731 5153-5154 CD denotes 1
T3730 5151-5152 , denotes ,
T3729 5147-5151 NNP denotes EDTA
T3728 5144-5146 NNP denotes mM
T3727 5142-5143 CD denotes 1
T3726 5140-5141 , denotes ,
T3725 5137-5140 NNP denotes SDS
T3724 5135-5136 NN denotes %
T3723 5132-5135 CD denotes 0.1
T3722 5130-5131 , denotes ,
T3721 5118-5130 NN denotes deoxycholate
T3720 5111-5117 NN denotes sodium
T3719 5109-5110 NN denotes %
T3718 5106-5109 CD denotes 0.5
T3717 5104-5105 , denotes ,
T3716 5100-5104 CD denotes NP40
T3715 5098-5099 NN denotes %
T3714 5097-5098 CD denotes 1
T3713 5096-5097 -LRB- denotes (
T3712 5089-5095 NN denotes buffer
T3711 5084-5088 NNP denotes RIPA
T3710 5081-5083 IN denotes in
T3709 5069-5080 VBN denotes homogenized
T3708 5064-5068 VBD denotes were
T3707 5058-5063 NNS denotes cells
T3706 5056-5057 , denotes ,
T3705 5049-5056 VBZ denotes lysates
T3035 4403-4404 . denotes .
T3034 4397-4403 NNP denotes ImageJ
T3033 4388-4396 NN denotes software
T3032 4385-4387 IN denotes by
T3031 4374-4384 VBN denotes quantified
T3030 4369-4373 VBD denotes were
T3029 4360-4368 NN denotes activity
T3028 4352-4359 JJ denotes binding
T3027 4348-4351 NNP denotes DNA
T3026 4342-4347 NNP denotes NF-κB
T3025 4339-4341 IN denotes of
T3024 4332-4338 NNS denotes Levels
T3023 4330-4331 . denotes .
T3022 4329-4330 -RRB- denotes )
T3021 4324-4329 NNP denotes Japan
T3020 4322-4323 , denotes ,
T3019 4317-4322 NNP denotes Tokyo
T3018 4315-4316 , denotes ,
T3017 4306-4315 NNP denotes Hyperfilm
T3016 4301-4305 NNP denotes Fuji
T3015 4300-4301 -LRB- denotes (
T3014 4295-4299 NN denotes film
T3013 4289-4294 NN denotes X-ray
T3012 4286-4288 TO denotes to
T3011 4278-4285 VBN denotes exposed
T3010 4274-4277 CC denotes and
T3009 4268-4273 VBN denotes dried
T3008 4264-4267 VBD denotes was
T3007 4260-4263 NN denotes gel
T3006 4256-4259 DT denotes the
T3005 4254-4255 , denotes ,
T3004 4239-4254 NN denotes electrophoresis
T3003 4233-4238 IN denotes After
T3002 4231-4232 . denotes .
T3001 4230-4231 NNP denotes C
T3000 4229-4230 CD denotes °
T2999 4228-4229 CD denotes 4
T2998 4225-4227 IN denotes at
T2997 4220-4224 NN denotes hour
T2996 4218-4219 CD denotes 1
T2995 4214-4217 IN denotes for
T2994 4212-4213 NN denotes V
T2993 4208-4211 CD denotes 390
T2992 4205-4207 IN denotes at
T2991 4203-4204 -RRB- denotes )
T2990 4187-4203 NNP denotes tris-borate-EDTA
T2989 4186-4187 -LRB- denotes (
T2988 4179-4185 NN denotes buffer
T2987 4175-4178 NNP denotes TBE
T2986 4173-4174 CD denotes ×
T2985 4169-4172 CD denotes 0.5
T2984 4166-4168 IN denotes in
T2983 4162-4165 NN denotes gel
T2982 4147-4161 NN denotes polyacrylamide
T2981 4133-4146 JJ denotes nondenaturing
T2980 4131-4132 NN denotes %
T2979 4130-4131 CD denotes 4
T2978 4127-4129 IN denotes on
T2977 4111-4126 NN denotes electrophoresis
T2976 4108-4110 IN denotes by
T2975 4098-4107 VBN denotes separated
T2974 4093-4097 VBD denotes were
T2973 4083-4092 NNS denotes complexes
T2972 4071-4082 JJ denotes DNA-protein
T2971 4067-4070 DT denotes The
T2970 4065-4066 . denotes .
T2969 4062-4065 NN denotes ice
T2968 4059-4061 IN denotes on
T2967 4051-4058 NNS denotes minutes
T2966 4048-4050 CD denotes 20
T2965 4044-4047 IN denotes for
T2964 4034-4043 VBN denotes continued
T2963 4030-4033 VBD denotes was
T2962 4019-4029 NN denotes incubation
T2961 4015-4018 DT denotes the
T2960 4013-4014 , denotes ,
T2959 4008-4013 NN denotes probe
T2958 3992-4007 NN denotes oligonucleotide
T2957 3981-3991 JJ denotes 32P-labled
T2956 3978-3980 NNP denotes μL
T2955 3976-3977 CD denotes 1
T2954 3972-3975 DT denotes the
T2953 3969-3971 IN denotes of
T2952 3960-3968 NN denotes addition
T2951 3954-3959 IN denotes After
T2950 3952-3953 . denotes .
T2949 3949-3952 NN denotes ice
T2948 3946-3948 IN denotes on
T2947 3938-3945 NNS denotes minutes
T2946 3935-3937 CD denotes 20
T2945 3931-3934 IN denotes for
T2944 3925-3930 NN denotes dI-dC
T2943 3920-3924 NN denotes poly
T2942 3917-3919 NN denotes μg
T2941 3915-3916 CD denotes 2
T2940 3911-3914 CC denotes and
T2939 3909-3910 , denotes ,
T2938 3906-3909 NNP denotes DTT
T2937 3903-3905 NNP denotes mM
T2936 3899-3902 CD denotes 0.5
T2935 3897-3898 , denotes ,
T2934 3893-3897 NNP denotes EDTA
T2933 3890-3892 NNP denotes mM
T2932 3886-3889 CD denotes 0.5
T2931 3884-3885 , denotes ,
T2930 3876-3884 NN denotes glycerol
T2929 3874-3875 NN denotes %
T2928 3873-3874 CD denotes 4
T2927 3871-3872 , denotes ,
T2926 3867-3871 NNP denotes NaCl
T2925 3864-3866 NNP denotes mM
T2924 3860-3863 CD denotes 0.5
T2923 3858-3859 , denotes ,
T2922 3853-3858 NNP denotes MgCl2
T2921 3850-3852 NNP denotes mM
T2920 3848-3849 CD denotes 1
T2919 3846-3847 , denotes ,
T2918 3845-3846 -RRB- denotes )
T2917 3842-3845 CD denotes 7.5
T2916 3839-3841 NNP denotes PH
T2915 3838-3839 -LRB- denotes (
T2914 3829-3837 NNP denotes Tris-HCl
T2913 3826-3828 NNP denotes mM
T2912 3823-3825 CD denotes 10
T2911 3812-3822 VBG denotes containing
T2910 3805-3811 NN denotes buffer
T2909 3797-3804 JJ denotes binding
T2908 3794-3796 NN denotes μL
T2907 3791-3793 CD denotes 20
T2906 3788-3790 IN denotes in
T2905 3775-3787 VBN denotes preincubated
T2904 3771-3774 VBD denotes was
T2903 3769-3770 -RRB- denotes )
T2902 3767-3769 NN denotes μg
T2901 3764-3766 CD denotes 20
T2900 3763-3764 -LRB- denotes (
T2899 3755-3762 NN denotes protein
T2898 3747-3754 NNP denotes Nuclear
T2897 3745-3746 . denotes .
T2896 3744-3745 -RRB- denotes )
T2895 3739-3744 NNP denotes China
T2894 3737-3738 , denotes ,
T2893 3730-3737 NNP denotes Beijing
T2892 3728-3729 , denotes ,
T2891 3720-3728 NNP denotes Biotech.
T2890 3715-3719 NNP denotes Free
T2889 3714-3715 -LRB- denotes (
T2888 3710-3713 NNP denotes ATP
T2887 3709-3710 : denotes -
T2886 3708-3709 NNP denotes ]
T2885 3703-3708 NNP denotes γ-32P
T2884 3702-3703 NNP denotes [
T2883 3696-3701 VBG denotes using
T2882 3689-3695 NN denotes kinase
T2881 3671-3688 NN denotes T4-polynucleotide
T2880 3668-3670 IN denotes by
T2879 3656-3667 VBN denotes end-labeled
T2878 3652-3655 VBD denotes was
T2877 3650-3651 -RRB- denotes )
T2876 3649-3650 NN denotes ′
T2875 3625-3649 NN denotes AGTTGAGGGGACTTTCCCAGGG-3
T2874 3624-3625 : denotes -
T2873 3623-3624 SYM denotes ′
T3968 6399-6404 NNP denotes Japan
T3967 6397-6398 , denotes ,
T3966 6392-6397 NNP denotes Tokyo
T3349 5004-5005 . denotes .
T3348 4996-5004 NN denotes standard
T3347 4987-4995 JJ denotes internal
T3346 4984-4986 DT denotes an
T3345 4981-4983 IN denotes as
T3344 4976-4980 VBN denotes used
T3343 4972-4975 VBD denotes was
T3342 4964-4971 NN denotes β-actin
T3341 4961-4963 IN denotes of
T3340 4955-4960 NN denotes level
T3339 4951-4954 DT denotes The
T3338 4949-4950 . denotes .
T3337 4942-4949 NN denotes program
T3336 4935-4941 NNP denotes ImageJ
T3335 4932-4934 IN denotes by
T3334 4923-4931 VBN denotes analyzed
T3333 4919-4922 VBD denotes was
T3332 4913-4918 NNS denotes bands
T3331 4909-4912 DT denotes the
T3330 4906-4908 IN denotes of
T3329 4896-4905 NN denotes intensity
T3328 4892-4895 DT denotes The
T3327 4890-4891 . denotes .
T3326 4875-4890 NN denotes electrophoresis
T3325 4871-4874 NN denotes gel
T3324 4863-4870 JJ denotes agarose
T3323 4860-4862 IN denotes by
T3322 4851-4859 VBN denotes detected
T3321 4846-4850 VBD denotes were
T3320 4837-4845 NNS denotes products
T3319 4833-4836 NNP denotes PCR
T3318 4831-4832 . denotes .
T3317 4821-4831 NNS denotes parameters
T3316 4817-4820 NNP denotes PCR
T3315 4813-4816 CC denotes and
T3314 4805-4812 NNS denotes primers
T3313 4801-4804 DT denotes the
T3312 4795-4800 VBZ denotes shows
T3311 4793-4794 CD denotes 1
T3310 4787-4792 NNP denotes Table
T3309 4784-4785 . denotes .
T3308 4776-4784 NN denotes protocol
T3307 4773-4775 POS denotes 's
T3306 4761-4773 NN denotes manufacturer
T3305 4757-4760 DT denotes the
T3304 4754-4756 TO denotes to
T3303 4744-4753 VBG denotes according
T3302 4742-4743 -RRB- denotes )
T3301 4739-4742 NNP denotes USA
T3300 4737-4738 , denotes ,
T3299 4735-4737 NNP denotes WI
T3298 4733-4734 , denotes ,
T3297 4726-4733 NNP denotes Promega
T3296 4725-4726 -LRB- denotes (
T3295 4721-4724 NNP denotes Mix
T3294 4714-4720 NNP denotes Master
T3293 4708-4713 NNP denotes Green
T3292 4702-4707 NNP denotes GoTaq
T3291 4697-4701 IN denotes with
T3290 4687-4696 VBN denotes conducted
T2872 3622-3623 CD denotes 5
T2871 3621-3622 -LRB- denotes (
T2870 3615-3620 NN denotes probe
T2869 3599-3614 NN denotes oligonucleotide
T2868 3589-3598 NNP denotes Consensus
T2867 3587-3588 . denotes .
T2866 3586-3587 -RRB- denotes )
T2865 3583-3586 NNP denotes USA
T2864 3581-3582 , denotes ,
T2863 3579-3581 NNP denotes WI
T2862 3577-3578 , denotes ,
T2861 3570-3577 NNP denotes Madison
T2860 3568-3569 , denotes ,
T2859 3561-3568 NNP denotes Promega
T2858 3560-3561 -LRB- denotes (
T2857 3553-3559 NN denotes system
T2856 3547-3552 NN denotes assay
T2855 3541-3546 NN denotes shift
T2854 3537-3540 NN denotes gel
T2853 3531-3536 VBG denotes using
T2852 3521-3530 VBN denotes performed
T2851 3517-3520 VBD denotes was
T2850 3512-3516 NNP denotes EMSA
T2849 3510-3511 . denotes .
T2848 3509-3510 -RRB- denotes )
T2847 3506-3509 NNP denotes USA
T2772 3112-3113 -RRB- denotes )
T2771 3109-3112 CD denotes 7.9
T2770 3106-3108 NNP denotes pH
T2769 3105-3106 -LRB- denotes (
T2768 3099-3104 NNP denotes HEPES
T2767 3096-3098 NNP denotes mM
T2766 3093-3095 CD denotes 20
T2765 3082-3092 VBG denotes containing
T2764 3075-3081 NN denotes buffer
T2763 3070-3074 IN denotes with
T2762 3060-3069 VBN denotes extracted
T2761 3055-3059 VBD denotes were
T2760 3046-3054 NNS denotes proteins
T2759 3038-3045 JJ denotes nuclear
T2758 3034-3037 DT denotes The
T2757 3032-3033 . denotes .
T2756 3018-3032 NN denotes centrifugation
T2755 3015-3017 IN denotes by
T2754 3006-3014 VBN denotes pelleted
T2753 3001-3005 VBD denotes were
T2752 2994-3000 NNS denotes nuclei
T2751 2990-2993 DT denotes the
T2750 2986-2989 CC denotes and
T2749 2984-2985 , denotes ,
T2748 2977-2984 NN denotes briefly
T2747 2968-2976 VBN denotes vortexed
T2746 2964-2967 CC denotes and
T2745 2958-2963 VBN denotes added
T2744 2954-2957 VBD denotes was
T2743 2949-2953 NNP denotes P-40
T2742 2941-2948 NNP denotes Nonidet
T2741 2939-2940 NN denotes %
T2740 2937-2939 CD denotes 10
T2739 2932-2936 RB denotes Then
T2738 2930-2931 . denotes .
T2737 2922-2930 NN denotes fluoride
T2736 2901-2921 NN denotes phenylmethylsulfonyl
T2735 2898-2900 NNP denotes mM
T2734 2894-2897 CD denotes 0.5
T2733 2890-2893 CC denotes and
T2732 2888-2889 , denotes ,
T2731 2885-2888 NNP denotes DTT
T2730 2882-2884 NNP denotes mM
T2729 2878-2881 CD denotes 1.0
T2728 2876-2877 , denotes ,
T2727 2872-2876 NNP denotes EGTA
T2726 2869-2871 NNP denotes mM
T2725 2865-2868 CD denotes 0.1
T2724 2863-2864 , denotes ,
T2723 2859-2863 NNP denotes EDTA
T2722 2856-2858 NNP denotes mM
T2721 2852-2855 CD denotes 0.1
T2720 2850-2851 , denotes ,
T2719 2847-2850 NNP denotes KCl
T2718 2844-2846 NNP denotes mM
T2717 2841-2843 CD denotes 10
T2716 2839-2840 , denotes ,
T2715 2838-2839 -RRB- denotes )
T2714 2835-2838 CD denotes 7.9
T2713 2832-2834 NNP denotes pH
T2712 2831-2832 -LRB- denotes (
T2711 2825-2830 NNP denotes HEPES
T2710 2822-2824 NNP denotes mM
T2709 2819-2821 CD denotes 10
T2708 2808-2818 VBG denotes containing
T2707 2801-2807 NN denotes buffer
T2706 2798-2800 IN denotes in
T2705 2786-2797 VBN denotes resuspended
T2704 2782-2785 VBD denotes was
T2703 2767-2781 NN denotes centrifugation
T2702 2764-2766 IN denotes by
T2701 2755-2763 VBN denotes obtained
T2700 2748-2754 NN denotes pellet
T2699 2743-2747 NN denotes cell
T2698 2739-2742 DT denotes The
T2697 2737-2738 . denotes .
T2696 2734-2737 NNP denotes PBS
T2695 2729-2733 JJ denotes cold
T2694 2724-2728 IN denotes into
T2693 2715-2723 VBG denotes scraping
T2692 2712-2714 IN denotes by
T2691 2702-2711 VBN denotes harvested
T2690 2698-2701 CC denotes and
T2689 2694-2697 NNP denotes PBS
T2688 2689-2693 IN denotes with
T2687 2682-2688 VBN denotes washed
T2686 2677-2681 VBD denotes were
T2685 2666-2676 NNS denotes astrocytes
T2684 2663-2665 IN denotes of
T2683 2652-2662 NNPS denotes Monolayers
T2682 2650-2651 -RRB- denotes )
T2681 2646-2650 NNP denotes EMSA
T2680 2645-2646 -LRB- denotes (
T2679 2639-2644 NNP denotes Assay
T2678 2633-2638 NNP denotes Shift
T2677 2624-2632 NNP denotes Mobility
T2676 2608-2623 NNP denotes Electrophoretic
T2675 2606-2607 . denotes .
T2674 2603-2606 CD denotes 2.4
T3965 6390-6391 , denotes ,
T3964 6381-6390 NNP denotes Hyperfilm
T3963 6376-6380 NNP denotes Fuji
T3962 6375-6376 -LRB- denotes (
T3961 6370-6374 NN denotes film
T3960 6357-6369 JJ denotes radiographic
T3959 6354-6356 TO denotes to
T3958 6346-6353 VBN denotes exposed
T3957 6342-6345 CC denotes and
T3956 6340-6341 -RRB- denotes )
T3955 6338-6340 NNP denotes UK
T3954 6336-6337 , denotes ,
T3953 6331-6336 NNP denotes Bucks
T3952 6329-6330 , denotes ,
T3951 6318-6329 NNP denotes Biosciences
T3950 6309-6317 NNP denotes Amersham
T3949 6308-6309 -LRB- denotes (
T3948 6301-6307 NN denotes system
T3947 6291-6300 NN denotes detection
T3946 6289-6290 -RRB- denotes )
T3945 6286-6289 NNP denotes ECL
T3944 6285-6286 -LRB- denotes (
T3943 6267-6284 NN denotes chemiluminescence
T3942 6258-6266 JJ denotes enhanced
T3941 6253-6257 IN denotes with
T3940 6243-6252 VBN denotes incubated
T3939 6238-6242 VBD denotes were
T3938 6232-6237 NNS denotes Blots
T3937 6229-6231 NN denotes h.
T3936 6227-6228 CD denotes 1
T3935 6223-6226 IN denotes for
T3934 6214-6222 NN denotes dilution
T3933 6209-6213 CD denotes 2000
T3932 6207-6208 : denotes :
T3931 6205-6206 CD denotes 1
T3930 6203-6204 DT denotes a
T3929 6200-6202 IN denotes at
T3928 6191-6199 NN denotes antibody
T3927 6181-6190 JJ denotes secondary
T3926 6163-6180 JJ denotes peroxidase-linked
T3925 6151-6162 JJ denotes horseradish
T3924 6147-6150 DT denotes the
T3923 6142-6146 IN denotes with
T3922 6134-6141 VBN denotes covered
T3921 6130-6133 CC denotes and
T3920 6123-6129 NN denotes buffer
T3919 6115-6122 VBG denotes washing
T3918 6111-6114 DT denotes the
T3917 6108-6110 IN denotes in
T3916 6102-6107 NNS denotes times
T3915 6096-6101 CD denotes three
T3914 6089-6095 VBN denotes washed
T3913 6084-6088 VBD denotes were
T3912 6078-6083 NNS denotes blots
T3911 6074-6077 DT denotes The
T3910 6072-6073 . denotes .
T3909 6061-6072 NN denotes temperature
T3908 6056-6060 NN denotes room
T3907 6053-6055 IN denotes at
T3906 6051-6052 NN denotes h
T3905 6049-6050 CD denotes 1
T3904 6045-6048 IN denotes for
T3903 6039-6044 NNS denotes blots
T3902 6035-6038 DT denotes the
T3901 6032-6034 TO denotes to
T3900 6026-6031 VBD denotes added
T3899 6021-6025 RB denotes then
T3898 6017-6020 CC denotes and
T3897 6010-6016 NN denotes buffer
T3896 6001-6009 VBG denotes blocking
T3895 5998-6000 IN denotes in
T2846 3504-3505 , denotes ,
T2845 3502-3504 NNP denotes IL
T2844 3500-3501 , denotes ,
T2843 3492-3500 NNP denotes Rockford
T2842 3490-3491 , denotes ,
T2841 3478-3490 NNP denotes Biochemicals
T2840 3471-3477 NNP denotes Pierce
T2839 3470-3471 -LRB- denotes (
T2838 3461-3469 NN denotes standard
T2837 3457-3460 DT denotes the
T2836 3454-3456 IN denotes as
T2835 3446-3453 NN denotes albumin
T2834 3440-3445 NN denotes serum
T2833 3433-3439 JJ denotes bovine
T2832 3428-3432 IN denotes with
T2831 3424-3427 NN denotes kit
T2830 3418-3423 NN denotes assay
T2829 3413-3417 NN denotes acid
T2828 3399-3412 JJ denotes bicinchoninic
T2827 3397-3398 DT denotes a
T2826 3391-3396 VBG denotes using
T2825 3380-3390 VBN denotes determined
T2824 3376-3379 VBD denotes was
T2823 3362-3375 NN denotes concentration
T2822 3354-3361 NN denotes Protein
T2821 3352-3353 . denotes .
T2820 3349-3352 NN denotes use
T2819 3343-3348 IN denotes until
T2818 3341-3342 NNP denotes C
T2817 3340-3341 CD denotes °
T2816 3338-3340 CD denotes 80
T2815 3334-3336 IN denotes at
T2814 3327-3333 VBN denotes stored
T2813 3323-3326 VBD denotes was
T2812 3314-3322 NNS denotes proteins
T2811 3306-3313 JJ denotes nuclear
T2810 3302-3305 DT denotes the
T2809 3291-3301 VBG denotes containing
T2808 3279-3290 JJ denotes supernatant
T2807 3275-3278 DT denotes the
T2806 3271-3274 CC denotes and
T2805 3269-3270 , denotes ,
T2804 3266-3269 NN denotes rpm
T2803 3260-3265 CD denotes 14000
T2802 3257-3259 IN denotes at
T2801 3242-3256 NN denotes centrifugation
T2800 3239-3241 IN denotes by
T2799 3231-3238 VBN denotes removed
T2798 3227-3230 VBD denotes was
T2797 3218-3226 NN denotes material
T2796 3208-3217 NNP denotes Insoluble
T2795 3206-3207 . denotes .
T2794 3198-3206 NN denotes fluoride
T2793 3177-3197 NN denotes phenylmethylsulfonyl
T2792 3174-3176 NNP denotes mM
T2791 3170-3173 CD denotes 1.0
T2790 3166-3169 CC denotes and
T2789 3164-3165 , denotes ,
T2788 3161-3164 NNP denotes DTT
T2787 3158-3160 NNP denotes mM
T2786 3154-3157 CD denotes 1.0
T2785 3152-3153 , denotes ,
T2784 3148-3152 NNP denotes EGTA
T2783 3145-3147 NNP denotes mM
T2782 3141-3144 CD denotes 1.0
T2781 3139-3140 , denotes ,
T2780 3135-3139 NNP denotes EDTA
T2779 3132-3134 NNP denotes mM
T2778 3128-3131 CD denotes 1.0
T2777 3126-3127 , denotes ,
T2776 3122-3126 NNP denotes NaCl
T2775 3119-3121 NNP denotes mM
T2774 3115-3118 CD denotes 0.4
T2773 3113-3114 , denotes ,
T2294 2600-2601 . denotes .
T2293 2598-2600 NNP denotes SD
T2292 2596-2597 NN denotes ±
T2291 2593-2595 NN denotes nm
T2290 2589-2592 CD denotes 450
T2289 2586-2588 IN denotes at
T2288 2580-2585 NN denotes value
T2287 2577-2579 NNP denotes OD
T2286 2574-2576 IN denotes of
T2285 2569-2573 NN denotes mean
T2284 2566-2568 IN denotes as
T2283 2556-2565 VBN denotes expressed
T2282 2551-2555 VBD denotes were
T2281 2543-2550 NNS denotes Results
T2280 2541-2542 . denotes .
T2279 2529-2541 NN denotes sextuplicate
T2278 2526-2528 IN denotes in
T2260 2427-2428 DT denotes a
T2259 2421-2426 VBG denotes using
T2258 2418-2420 NN denotes nm
T2257 2414-2417 CD denotes 450
T2256 2411-2413 IN denotes at
T2253 2396-2401 NN denotes value
T2252 2393-2395 NNP denotes OD
T2251 2388-2392 RB denotes Then
T2250 2386-2387 . denotes .
T2249 2385-2386 NN denotes h
T2248 2383-2384 CD denotes 1
T2247 2379-2382 IN denotes for
T2246 2369-2378 VBN denotes incubated
T2245 2365-2368 CC denotes and
T2244 2360-2364 NN denotes well
T2243 2354-2359 DT denotes every
T2242 2349-2353 IN denotes into
T2241 2343-2348 VBN denotes added
T2240 2339-2342 VBD denotes was
T2239 2333-2338 NNP denotes CCK-8
T2238 2330-2332 NNP denotes μL
T2237 2327-2329 CD denotes 10
T2236 2325-2326 , denotes ,
T2235 2318-2325 RB denotes Briefly
T2234 2316-2317 . denotes .
T2233 2308-2316 NN denotes protocol
T2232 2305-2307 POS denotes 's
T2231 2293-2305 NN denotes manufacturer
T2230 2289-2292 DT denotes the
T2229 2286-2288 TO denotes to
T2228 2276-2285 VBG denotes according
T2227 2268-2275 NN denotes scratch
T2226 2262-2267 IN denotes after
T2225 2260-2261 NN denotes h
T2224 2257-2259 CD denotes 24
T2223 2251-2256 NN denotes assay
T2222 2249-2250 -RRB- denotes )
T2221 2244-2249 NNP denotes Japan
T2220 2242-2243 , denotes ,
T2219 2235-2242 NNP denotes Dojindo
T2218 2234-2235 -LRB- denotes (
T2217 2232-2233 -RRB- denotes )
T2216 2227-2232 NN denotes CCK-8
T2215 2226-2227 -LRB- denotes (
T2214 2220-2225 JJ denotes kit-8
T2213 2211-2219 VBG denotes counting
T2212 2206-2210 NN denotes cell
T2211 2203-2205 IN denotes by
T2210 2194-2202 VBN denotes assessed
T2209 2190-2193 VBD denotes was
T2208 2184-2189 NN denotes death
T2207 2179-2183 NN denotes cell
T2206 2169-2178 NNP denotes Astrocyte
T2205 2167-2168 . denotes .
T2204 2162-2167 IN denotes above
T2203 2152-2161 VBN denotes mentioned
T2202 2149-2151 IN denotes as
T2201 2141-2148 VBN denotes scrated
T2200 2137-2140 VBD denotes was
T2199 2132-2136 NN denotes cell
T2198 2128-2131 DT denotes the
T2197 2123-2127 RB denotes Then
T2196 2121-2122 . denotes .
T2195 2115-2121 VB denotes adhere
T2194 2112-2114 TO denotes to
T2193 2110-2111 NN denotes h
T2192 2107-2109 CD denotes 24
T2191 2103-2106 IN denotes for
T2190 2092-2102 VBN denotes cultivated
T2189 2088-2091 CC denotes and
T2188 2081-2087 NNS denotes plates
T2187 2073-2080 NN denotes culture
T2186 2065-2072 JJ denotes 96-well
T2185 2062-2064 TO denotes to
T2184 2055-2061 VBN denotes seeded
T2183 2050-2054 VBD denotes were
T2182 2044-2049 NNS denotes cells
T2181 2035-2043 JJ denotes 103/well
T2180 2033-2034 CD denotes ×
T2179 2031-2032 CD denotes 1
T2178 2025-2030 NNP denotes Assay
T2177 2019-2024 NNP denotes CCK-8
T2176 2016-2018 IN denotes by
T2175 2004-2015 NNP denotes Measurement
T2174 1998-2003 NNP denotes Death
T2173 1993-1997 NNP denotes Cell
T2172 1991-1992 . denotes .
T2171 1988-1991 CD denotes 2.3
T1737 1180-1183 CC denotes and
T1736 1170-1179 NN denotes microglia
T1735 1166-1169 DT denotes the
T1734 1158-1165 VB denotes deplete
T1733 1155-1157 TO denotes to
T1732 1153-1154 NN denotes h
T1731 1151-1152 CD denotes 4
T1730 1147-1150 IN denotes for
T1729 1143-1146 NN denotes rpm
T1728 1139-1142 CD denotes 150
T1727 1136-1138 IN denotes at
T1726 1129-1135 VBN denotes shaken
T1725 1124-1128 VBD denotes were
T1724 1117-1123 NNS denotes dishes
T1723 1114-1116 CC denotes or
T1722 1107-1113 NNS denotes flasks
T1721 1103-1106 DT denotes the
T1720 1098-1102 RB denotes Then
T1719 1096-1097 . denotes .
T1718 1086-1096 NN denotes confluence
T1717 1080-1085 VB denotes reach
T1716 1077-1079 TO denotes to
T1715 1072-1076 NNS denotes days
T1714 1070-1071 CD denotes 7
T1713 1064-1069 IN denotes about
T1712 1060-1063 IN denotes for
T1711 1051-1059 VBD denotes expanded
T1710 1040-1050 NNP denotes Astrocytes
T1709 1038-1039 . denotes .
T1708 1034-1038 NN denotes week
T1707 1032-1033 DT denotes a
T1706 1026-1031 RB denotes twice
T1705 1013-1025 JJ denotes half-changed
T1704 1009-1012 VBD denotes was
T1703 1002-1008 NN denotes medium
T1702 994-1001 NN denotes culture
T1701 985-993 JJ denotes complete
T1700 981-984 DT denotes the
T1699 977-980 CC denotes And
T1698 975-976 . denotes .
T1697 972-975 CD denotes CO2
T1696 970-971 NN denotes %
T1695 969-970 CD denotes 5
T1694 964-968 IN denotes with
T1693 962-963 NNP denotes C
T1692 961-962 CD denotes °
T1691 959-961 CD denotes 37
T1690 956-958 IN denotes at
T1689 945-955 VBN denotes cultivated
T1688 940-944 VBD denotes were
T1687 934-939 NNS denotes cells
T1686 930-933 DT denotes the
T1685 925-929 RB denotes Then
T1684 923-924 . denotes .
T1683 918-923 NNS denotes cells
T1682 907-917 JJ denotes epithelial
T1681 898-906 JJ denotes residual
T1680 894-897 DT denotes the
T1679 884-893 VBG denotes depleting
T1678 880-883 IN denotes for
T1677 878-879 -RRB- denotes )
T1676 875-878 NNP denotes USA
T1675 873-874 , denotes ,
T1674 867-873 NNP denotes Costar
T1673 866-867 -LRB- denotes (
T1672 861-865 NN denotes dish
T1671 858-860 CC denotes or
T1670 852-857 NN denotes flask
T1669 848-851 JJ denotes new
T1668 846-847 DT denotes a
T1667 843-845 TO denotes to
T1666 831-842 VBN denotes transferred
T1665 827-830 VBD denotes was
T1664 815-826 NN denotes supernatant
T1663 811-814 DT denotes the
T1662 809-810 , denotes ,
T1661 808-809 NN denotes h
T1660 806-807 CD denotes 1
T1659 802-805 IN denotes for
T1658 791-801 NN denotes incubation
T1657 785-790 IN denotes After
T1656 783-784 . denotes .
T1655 782-783 -RRB- denotes )
T1654 776-782 NN denotes medium
T1653 768-775 NN denotes culture
T1652 759-767 JJ denotes complete
T1651 758-759 -LRB- denotes (
T1650 745-757 NNP denotes streptomycin
T1649 742-744 NNP denotes mL
T1648 741-742 NN denotes /
T1647 739-741 NN denotes μg
T1646 737-738 CD denotes 5
T1645 735-736 , denotes ,
T1644 725-735 NN denotes penicillin
T1643 716-724 JJ denotes units/mL
T1642 714-715 CD denotes 5
T1641 710-713 CC denotes and
T1640 706-709 NNP denotes FBS
T1639 704-705 NN denotes %
T1638 702-704 CD denotes 20
T1637 697-701 IN denotes with
T1636 692-696 NNP denotes DMEM
T1635 689-691 IN denotes in
T1634 676-688 NN denotes dissociation
T1633 665-675 JJ denotes mechanical
T1632 663-664 DT denotes a
T1631 660-662 IN denotes by
T1630 651-659 VBN denotes followed
T1629 649-650 , denotes ,
T1628 646-649 NNP denotes FBS
T1627 644-645 NN denotes %
T1626 642-644 CD denotes 10
T1625 637-641 IN denotes with
T1624 632-636 NNP denotes DMEM
T1623 629-631 IN denotes in
T1622 623-628 RB denotes twice
T1621 616-622 VBN denotes rinsed
T1620 612-615 VBD denotes was
T1619 605-611 NN denotes tissue
T1618 601-604 DT denotes the
T1617 599-600 , denotes ,
T1616 590-599 NN denotes digestion
T1615 584-589 IN denotes After
T1614 582-583 . denotes .
T1613 575-582 NNS denotes minutes
T1612 571-574 JJ denotes few
T1611 565-570 DT denotes every
T1610 556-564 VBD denotes agitated
T1609 552-555 CC denotes and
T1608 550-551 , denotes ,
T1607 549-550 NNP denotes C
T1606 548-549 CD denotes °
T1605 546-548 CD denotes 37
T1604 543-545 IN denotes at
T1603 535-542 NNS denotes minutes
T1602 532-534 CD denotes 10
T1601 528-531 IN denotes for
T1600 520-527 NN denotes trypsin
T1599 518-519 NN denotes %
T1598 515-518 CD denotes 0.5
T1597 512-514 IN denotes in
T1596 502-511 VBN denotes incubated
T1595 500-501 , denotes ,
T1594 495-500 NN denotes blade
T1593 487-494 NN denotes scalpel
T1592 485-486 DT denotes a
T1591 480-484 IN denotes with
T1574 384-387 RP denotes out
T1573 374-383 VBN denotes dissected
T1572 369-373 VBD denotes were
T1571 360-368 NNS denotes cortices
T1570 356-359 DT denotes the
T1569 354-355 , denotes ,
T1568 342-354 NN denotes decapitation
T1567 332-341 VBG denotes Following
T1566 330-331 . denotes .
T1565 329-330 -RRB- denotes )
T1564 321-329 NN denotes genotype
T1563 316-320 DT denotes each
T1562 312-315 IN denotes for
T1561 307-311 NNS denotes mice
T1560 305-306 CD denotes 6
T1559 304-305 -LRB- denotes (
T1558 299-303 NNS denotes mice
T1557 297-298 -RRB- denotes )
T1556 295-297 NNP denotes KO
T1555 294-295 -LRB- denotes (
T1554 285-293 NN denotes knockout
T1553 281-284 CC denotes and
T1552 279-280 -RRB- denotes )
T1551 277-279 NNP denotes WT
T1550 276-277 -LRB- denotes (
T1549 271-275 NN denotes type
T1548 266-270 JJ denotes wide
T2277 2516-2525 VBN denotes performed
T2276 2511-2515 VBD denotes were
T2275 2498-2510 NNS denotes measurements
T2274 2494-2497 DT denotes All
T2273 2492-2493 . denotes .
T2272 2491-2492 -RRB- denotes )
T2271 2488-2491 NNP denotes USA
T2270 2486-2487 , denotes ,
T2269 2484-2486 NNP denotes CA
T2268 2482-2483 , denotes ,
T2267 2470-2482 NNPS denotes Laboratories
T2266 2462-2469 NNP denotes Bio-Rad
T2265 2461-2462 -LRB- denotes (
T2264 2454-2460 NN denotes reader
T2263 2443-2453 NN denotes microplate
T2262 2437-2442 NNP denotes ELISA
T2261 2429-2436 NNP denotes Bio-Rad
T1547 261-265 JJ denotes Nrf2
T1546 251-260 JJ denotes 2-day-old
T1545 241-250 JJ denotes postnatal
T1544 236-240 IN denotes from
T1543 227-235 VBN denotes obtained
T1542 222-226 VBD denotes were
T1541 211-221 NNS denotes astrocytes
T1540 203-210 JJ denotes Primary
T1539 201-202 . denotes .
T1538 200-201 -RRB- denotes )
T1537 197-200 NNP denotes USA
T1536 195-196 , denotes ,
T1535 193-195 NNP denotes MD
T1534 191-192 , denotes ,
T1533 182-191 NNP denotes Baltimore
T1532 180-181 , denotes ,
T1531 170-180 NNP denotes University
T1530 162-169 NNP denotes Hopkins
T1529 156-161 NNP denotes Johns
T1528 155-156 -LRB- denotes (
T1527 147-154 NNP denotes Kensler
T1526 144-146 NNP denotes W.
T1525 137-143 NNP denotes Thomas
T1524 133-136 NNP denotes Dr.
T1523 130-132 IN denotes by
T1927 1479-1482 NNP denotes TBI
T1926 1476-1478 IN denotes of
T1925 1470-1475 NNP denotes Model
T1924 1464-1469 NNP denotes Vitro
T1923 1461-1463 IN denotes In
T1922 1459-1460 . denotes .
T1921 1456-1459 CD denotes 2.2
T1785 1453-1454 . denotes .
T1784 1446-1453 NNS denotes studies
T1783 1436-1445 JJ denotes following
T1782 1432-1435 DT denotes the
T1781 1429-1431 IN denotes in
T1780 1424-1428 VBN denotes used
T1779 1419-1423 VBD denotes were
T1778 1407-1418 NNS denotes generations
T1777 1403-1406 CD denotes 2-3
T1776 1399-1402 IN denotes for
T1775 1390-1398 VBD denotes passaged
T1774 1384-1389 NNS denotes Cells
T1773 1382-1383 . denotes .
T1772 1381-1382 -RRB- denotes )
T1771 1378-1381 NNP denotes USA
T1770 1376-1377 , denotes ,
T1769 1374-1376 NNP denotes CA
T1768 1372-1373 , denotes ,
T1767 1359-1372 NNP denotes Biotechnology
T1766 1354-1358 NNP denotes Cruz
T1765 1348-1353 NNP denotes Santa
T1764 1346-1347 , denotes ,
T1763 1337-1346 JJ denotes sc-166481
T1762 1336-1337 -LRB- denotes (
T1761 1331-1335 NNP denotes GFAP
T1760 1327-1330 IN denotes for
T1759 1310-1326 NN denotes immunoreactivity
T1758 1307-1309 IN denotes by
T1757 1296-1306 VBN denotes determined
T1756 1291-1295 VBD denotes were
T1755 1285-1290 WDT denotes which
T1754 1283-1284 , denotes ,
T1753 1273-1283 NNS denotes astrocytes
T1752 1266-1272 RB denotes mainly
T1751 1261-1265 VBD denotes were
T1750 1252-1260 NNS denotes cultures
T1749 1242-1251 VBG denotes resulting
T1748 1238-1241 DT denotes the
T1747 1236-1237 , denotes ,
T1746 1229-1236 VBG denotes shaking
T1745 1223-1228 IN denotes After
T1744 1221-1222 . denotes .
T1743 1213-1221 NNS denotes cultures
T1742 1209-1212 DT denotes the
T1741 1204-1208 IN denotes from
T1740 1198-1203 NNS denotes cells
T1739 1189-1197 JJ denotes adherent
T1738 1184-1188 JJR denotes less
T2022 1985-1986 . denotes .
T2021 1980-1985 NN denotes group
T2020 1975-1979 NN denotes sham
T2019 1972-1974 IN denotes as
T2018 1966-1971 VBN denotes named
T2017 1962-1965 CC denotes and
T2016 1954-1961 NN denotes control
T2015 1948-1953 JJ denotes blank
T2014 1945-1947 IN denotes as
T2013 1940-1944 VBN denotes used
T2012 1935-1939 VBD denotes were
T2011 1933-1934 , denotes ,
T2010 1926-1933 NN denotes scratch
T2009 1917-1925 VBN denotes received
T2008 1913-1916 RB denotes not
T2007 1909-1912 VBD denotes did
T2006 1903-1908 WDT denotes which
T2005 1901-1902 , denotes ,
T2004 1896-1901 NNS denotes Cells
T2003 1894-1895 . denotes .
T2002 1889-1894 NN denotes group
T2001 1885-1888 NNP denotes TBI
T2000 1882-1884 IN denotes as
T1999 1876-1881 VBN denotes named
T1998 1872-1875 CC denotes and
T1997 1870-1871 , denotes ,
T1996 1864-1870 NN denotes medium
T1995 1856-1863 NN denotes culture
T1994 1847-1855 JJ denotes complete
T1993 1842-1846 IN denotes with
T1992 1833-1841 VBN denotes cultured
T1991 1831-1832 , denotes ,
T1990 1828-1831 NNP denotes PBS
T1989 1820-1827 JJ denotes sterile
T1988 1815-1819 IN denotes with
T1987 1809-1814 RB denotes twice
T1986 1802-1808 VBN denotes washed
T1985 1797-1801 VBD denotes were
T1984 1791-1796 NNS denotes cells
T1983 1789-1790 , denotes ,
T1982 1782-1789 NN denotes scratch
T1981 1776-1781 IN denotes after
T1980 1764-1775 RB denotes Immediately
T1979 1762-1763 . denotes .
T1978 1759-1762 NN denotes gap
T1977 1754-1758 JJ denotes wide
T1976 1751-1753 NN denotes mm
T1975 1747-1750 CD denotes 0.5
T1974 1745-1746 DT denotes a
T1973 1742-1744 IN denotes of
T1972 1732-1741 NN denotes formation
T1971 1728-1731 DT denotes the
T1970 1725-1727 IN denotes in
T1969 1715-1724 VBG denotes resulting
T1968 1713-1714 , denotes ,
T1967 1707-1713 NN denotes needle
T1966 1699-1706 NN denotes syringe
T1965 1695-1698 JJ denotes 26G
T1964 1687-1694 JJ denotes sterile
T1963 1685-1686 DT denotes a
T1962 1680-1684 IN denotes with
T1961 1670-1679 VBN denotes scratched
T1960 1666-1669 VBD denotes was
T1959 1656-1665 NN denotes monolayer
T1958 1651-1655 NN denotes cell
T1957 1647-1650 DT denotes The
T1956 1645-1646 . denotes .
T1955 1635-1645 NN denotes confluence
T1954 1632-1634 TO denotes to
T1953 1626-1631 VBN denotes grown
T1952 1622-1625 CC denotes and
T1951 1615-1621 NNS denotes plates
T1950 1608-1614 JJ denotes 6-well
T1949 1605-1607 IN denotes in
T1948 1597-1604 VBN denotes planted
T1947 1592-1596 VBD denotes were
T1946 1581-1591 NNS denotes Astrocytes
T1945 1579-1580 . denotes .
T1944 1578-1579 NNP denotes ]
T1943 1577-1578 CD denotes 7
T1942 1576-1577 NN denotes [
T1941 1569-1575 NN denotes report
T1940 1560-1568 JJ denotes previous
T1939 1558-1559 DT denotes a
T1938 1555-1557 IN denotes in
T1937 1552-1554 IN denotes as
T1936 1542-1551 VBN denotes performed
T1935 1538-1541 VBD denotes was
T1934 1531-1537 NN denotes injury
T1933 1523-1530 NN denotes scratch
T1932 1513-1522 NNP denotes Astrocyte
T1931 1506-1512 NNP denotes Injury
T1930 1498-1505 NNP denotes Scratch
T1929 1495-1497 IN denotes by
T1928 1483-1494 VBN denotes Established
T3800 5483-5485 IN denotes in
T3799 5468-5482 JJ denotes heat-denatured
T3798 5463-5467 VBD denotes were
T3797 5454-5462 NNS denotes extracts
T3796 5446-5453 NN denotes protein
T3795 5436-5445 JJ denotes cytosolic
T3794 5426-5435 VBG denotes resulting
T3793 5422-5425 DT denotes the
T3792 5419-5421 IN denotes of
T3791 5408-5418 NNS denotes micrograms
T3790 5402-5407 NNP denotes Fifty
T3789 5400-5401 . denotes .
T3788 5399-5400 -RRB- denotes )
T1522 121-129 VBN denotes provided
T1509 31-38 JJ denotes Primary
T1508 29-30 . denotes .
T1507 26-29 CD denotes 2.1
T1521 114-120 RB denotes kindly
T1520 109-113 VBD denotes were
T1519 104-108 NNS denotes mice
T1518 100-103 NNP denotes ICR
T1517 91-99 NN denotes knockout
T1516 86-90 NNP denotes Nrf2
T1515 75-85 NNP denotes Astrocytes
T1514 69-74 NNP denotes Mouse
T1513 66-68 IN denotes of
T1512 51-65 NNP denotes Identification
T1511 47-50 CC denotes and
T1510 39-46 NNP denotes Culture
T1590 472-479 VBN denotes chopped
T1589 464-471 RB denotes roughly
T1588 460-463 VBD denotes was
T1587 453-459 NN denotes tissue
T1586 449-452 DT denotes The
T1585 447-448 . denotes .
T1584 440-447 VBN denotes removed
T1583 435-439 VBD denotes were
T1582 427-434 NNS denotes vessels
T1581 421-426 NN denotes blood
T1580 410-420 VBN denotes associated
T1579 406-409 CC denotes and
T1578 397-405 NNS denotes meninges
T1577 393-396 DT denotes the
T1576 389-392 CC denotes and
T1575 387-388 , denotes ,
T3807 5527-5529 IN denotes by
T3806 5517-5526 VBN denotes separated
T3805 5515-5516 , denotes ,
T3804 5509-5515 NN denotes buffer
T3803 5501-5508 VBG denotes loading
T3802 5494-5500 NN denotes sample
T3801 5486-5493 NNP denotes Laemmli
T3890 5955-5962 JJ denotes primary
T3889 5950-5954 DT denotes Each
T3888 5948-5949 . denotes .
T3887 5947-5948 -RRB- denotes )
T3886 5944-5947 NN denotes kDa
T3885 5941-5943 CD denotes 92
T3884 5939-5940 , denotes ,
T3883 5936-5939 NNP denotes USA
T3882 5934-5935 , denotes ,
T3881 5932-5934 NNP denotes CA
T3880 5930-5931 , denotes ,
T3879 5917-5930 NNP denotes Biotechnology
T3878 5912-5916 NNP denotes Cruz
T3877 5906-5911 NNP denotes Santa
T3876 5904-5905 , denotes ,
T3875 5897-5904 JJ denotes sc-6841
T3874 5896-5897 -LRB- denotes (
T3873 5894-5895 -RRB- denotes )
T3872 5890-5894 NNP denotes MMP9
T3871 5889-5890 -LRB- denotes (
T3870 5887-5888 CD denotes 9
T3869 5870-5886 NN denotes metallopeptidase
T3868 5858-5869 JJ denotes anti-matrix
T3867 5854-5857 CC denotes and
T3866 5852-5853 -RRB- denotes )
T4681 8308-8309 , denotes ,
T4680 8303-8308 NN denotes water
T4679 8300-8302 IN denotes in
T4678 8292-8299 VBG denotes rinsing
T4677 8286-8291 IN denotes After
T4676 8284-8285 . denotes .
T4675 8277-8284 NN denotes gelatin
T4674 8273-8276 DT denotes the
T4673 8266-8272 VB denotes cleave
T4672 8263-8265 TO denotes to
T4671 8258-8262 NNP denotes MMP9
T4670 8254-8257 DT denotes the
T4669 8247-8253 VB denotes enable
T4668 8244-8246 TO denotes to
T4667 8242-8243 NNP denotes C
T4666 8241-8242 CD denotes °
T4665 8239-8241 CD denotes 37
T4664 8236-8238 IN denotes at
T4663 8234-8235 NN denotes h
T4662 8231-8233 CD denotes 40
T4661 8227-8230 IN denotes for
T4660 8220-8226 NN denotes buffer
T4659 8210-8219 JJ denotes substrate
T4658 8207-8209 IN denotes in
T4657 8197-8206 VBD denotes incubated
T4656 8192-8196 RB denotes then
T4655 8188-8191 CC denotes and
T4654 8182-8187 NNP denotes X-100
T4653 8175-8181 NNP denotes Triton
T4724 8546-8550 IN denotes with
T2255 2406-2410 VBN denotes read
T2254 2402-2405 VBD denotes was
R1247 T1507 T1507 ROOT 2.1,2.1
R1248 T1508 T1507 punct .,2.1
R1253 T1513 T1510 prep of,Culture
R1287 T1547 T1549 amod Nrf2,type
R1288 T1548 T1549 amod wide,type
R1289 T1549 T1558 nmod type,mice
R1290 T1550 T1549 punct (,type
R1291 T1551 T1549 appos WT,type
R1292 T1552 T1551 punct ),WT
R1293 T1553 T1549 cc and,type
R1294 T1554 T1549 conj knockout,type
R1295 T1555 T1556 punct (,KO
R1296 T1556 T1554 appos KO,knockout
R1297 T1557 T1554 punct ),knockout
R1298 T1558 T1544 pobj mice,from
R1299 T1559 T1558 punct (,mice
R1310 T1570 T1571 det the,cortices
R1311 T1571 T1573 nsubjpass cortices,dissected
R1312 T1572 T1573 auxpass were,dissected
R1313 T1573 T1573 ROOT dissected,dissected
R1314 T1574 T1573 prt out,dissected
R1315 T1575 T1573 punct ",",dissected
R1316 T1576 T1573 cc and,dissected
R1317 T1577 T1578 det the,meninges
R1318 T1578 T1580 nsubj meninges,associated
R1319 T1579 T1578 cc and,meninges
R1320 T1580 T1582 amod associated,vessels
R1321 T1581 T1582 compound blood,vessels
R1322 T1582 T1584 nsubjpass vessels,removed
R1323 T1583 T1584 auxpass were,removed
R1324 T1584 T1573 conj removed,dissected
R1325 T1585 T1584 punct .,removed
R1326 T1586 T1587 det The,tissue
R1327 T1587 T1590 nsubjpass tissue,chopped
R1328 T1588 T1590 auxpass was,chopped
R1329 T1589 T1590 advmod roughly,chopped
R1330 T1590 T1590 ROOT chopped,chopped
R1331 T1591 T1590 prep with,chopped
R1332 T1592 T1594 det a,blade
R1333 T1593 T1594 compound scalpel,blade
R1334 T1594 T1591 pobj blade,with
R1335 T1595 T1590 punct ",",chopped
R1336 T1596 T1590 conj incubated,chopped
R1337 T1597 T1596 prep in,incubated
R1338 T1598 T1599 nummod 0.5,%
R1339 T1599 T1600 compound %,trypsin
R1340 T1600 T1597 pobj trypsin,in
R1341 T1601 T1596 prep for,incubated
R1342 T1602 T1603 nummod 10,minutes
R1343 T1603 T1601 pobj minutes,for
R1344 T1604 T1596 prep at,incubated
R1345 T1605 T1606 nummod 37,°
R1346 T1606 T1607 nummod °,C
R1347 T1607 T1604 pobj C,at
R1348 T1608 T1596 punct ",",incubated
R1349 T1609 T1596 cc and,incubated
R1350 T1610 T1596 conj agitated,incubated
R1351 T1611 T1613 det every,minutes
R1352 T1612 T1613 amod few,minutes
R1353 T1613 T1590 npadvmod minutes,chopped
R1354 T1614 T1590 punct .,chopped
R1355 T1615 T1621 prep After,rinsed
R1356 T1616 T1615 pobj digestion,After
R1357 T1617 T1621 punct ",",rinsed
R1358 T1618 T1619 det the,tissue
R1359 T1619 T1621 nsubjpass tissue,rinsed
R1360 T1620 T1621 auxpass was,rinsed
R1361 T1621 T1621 ROOT rinsed,rinsed
R1362 T1622 T1621 advmod twice,rinsed
R1363 T1623 T1621 prep in,rinsed
R1364 T1624 T1623 pobj DMEM,in
R1365 T1625 T1621 prep with,rinsed
R1366 T1626 T1627 nummod 10,%
R1367 T1627 T1628 compound %,FBS
R1368 T1628 T1625 pobj FBS,with
R1369 T1629 T1628 punct ",",FBS
R1370 T1630 T1628 acl followed,FBS
R1371 T1631 T1630 agent by,followed
R1372 T1632 T1634 det a,dissociation
R1373 T1633 T1634 amod mechanical,dissociation
R1374 T1634 T1631 pobj dissociation,by
R1375 T1635 T1634 prep in,dissociation
R1376 T1636 T1635 pobj DMEM,in
R1386 T1646 T1647 nummod 5,μg
R1387 T1647 T1648 compound μg,/
R1388 T1648 T1650 compound /,streptomycin
R1389 T1649 T1650 compound mL,streptomycin
R1390 T1650 T1644 appos streptomycin,penicillin
R1391 T1651 T1654 punct (,medium
R1392 T1652 T1654 amod complete,medium
R1393 T1653 T1654 compound culture,medium
R1394 T1654 T1650 appos medium,streptomycin
R1395 T1655 T1650 punct ),streptomycin
R1396 T1656 T1621 punct .,rinsed
R1397 T1657 T1666 prep After,transferred
R1398 T1658 T1657 pobj incubation,After
R1399 T1659 T1658 prep for,incubation
R1400 T1660 T1661 nummod 1,h
R1401 T1661 T1659 pobj h,for
R1402 T1662 T1666 punct ",",transferred
R1403 T1663 T1664 det the,supernatant
R1404 T1664 T1666 nsubjpass supernatant,transferred
R1405 T1665 T1666 auxpass was,transferred
R1406 T1666 T1666 ROOT transferred,transferred
R1407 T1667 T1666 prep to,transferred
R1408 T1668 T1670 det a,flask
R1409 T1669 T1670 amod new,flask
R1410 T1670 T1667 pobj flask,to
R1411 T1671 T1670 cc or,flask
R1412 T1672 T1670 conj dish,flask
R1413 T1673 T1674 punct (,Costar
R1414 T1674 T1670 appos Costar,flask
R1415 T1675 T1674 punct ",",Costar
R1416 T1676 T1674 appos USA,Costar
R1417 T1677 T1674 punct ),Costar
R1418 T1678 T1666 prep for,transferred
R1419 T1679 T1678 pcomp depleting,for
R1420 T1680 T1683 det the,cells
R1421 T1681 T1683 amod residual,cells
R1422 T1682 T1683 amod epithelial,cells
R1423 T1683 T1679 dobj cells,depleting
R1424 T1684 T1666 punct .,transferred
R1425 T1685 T1689 advmod Then,cultivated
R1426 T1686 T1687 det the,cells
R1427 T1687 T1689 nsubjpass cells,cultivated
R1428 T1688 T1689 auxpass were,cultivated
R1429 T1689 T1689 ROOT cultivated,cultivated
R1430 T1690 T1689 prep at,cultivated
R1431 T1691 T1692 nummod 37,°
R1432 T1692 T1690 pobj °,at
R1433 T1693 T1689 npadvmod C,cultivated
R1434 T1694 T1689 prep with,cultivated
R1435 T1695 T1696 nummod 5,%
R1436 T1696 T1697 compound %,CO2
R1437 T1697 T1694 pobj CO2,with
R1438 T1698 T1689 punct .,cultivated
R1439 T1699 T1704 cc And,was
R1440 T1700 T1703 det the,medium
R1441 T1701 T1703 amod complete,medium
R1442 T1702 T1703 compound culture,medium
R1443 T1703 T1704 nsubj medium,was
R1444 T1704 T1704 ROOT was,was
R1445 T1705 T1704 acomp half-changed,was
R1446 T1706 T1704 advmod twice,was
R1447 T1707 T1708 det a,week
R1448 T1708 T1706 npadvmod week,twice
R1449 T1709 T1704 punct .,was
R1450 T1710 T1711 nsubj Astrocytes,expanded
R1451 T1711 T1711 ROOT expanded,expanded
R1452 T1712 T1711 prep for,expanded
R1453 T1713 T1714 advmod about,7
R1461 T1721 T1722 det the,flasks
R1462 T1722 T1726 nsubjpass flasks,shaken
R1463 T1723 T1722 cc or,flasks
R1464 T1724 T1722 conj dishes,flasks
R1465 T1725 T1726 auxpass were,shaken
R1466 T1726 T1726 ROOT shaken,shaken
R1467 T1727 T1726 prep at,shaken
R1468 T1728 T1729 nummod 150,rpm
R1469 T1729 T1727 pobj rpm,at
R1470 T1730 T1726 prep for,shaken
R1471 T1731 T1732 nummod 4,h
R1472 T1732 T1730 pobj h,for
R1473 T1733 T1734 aux to,deplete
R1474 T1734 T1726 advcl deplete,shaken
R1475 T1735 T1736 det the,microglia
R1476 T1736 T1734 dobj microglia,deplete
R1477 T1737 T1736 cc and,microglia
R1478 T1738 T1739 advmod less,adherent
R1479 T1739 T1740 amod adherent,cells
R1480 T1740 T1736 conj cells,microglia
R1481 T1741 T1734 prep from,deplete
R1482 T1742 T1743 det the,cultures
R1483 T1743 T1741 pobj cultures,from
R1484 T1744 T1726 punct .,shaken
R1485 T1745 T1751 prep After,were
R1486 T1746 T1745 pcomp shaking,After
R1487 T1747 T1751 punct ",",were
R1488 T1748 T1750 det the,cultures
R1489 T1749 T1750 amod resulting,cultures
R1490 T1750 T1751 nsubj cultures,were
R1491 T1751 T1751 ROOT were,were
R1492 T1752 T1753 advmod mainly,astrocytes
R1493 T1753 T1751 attr astrocytes,were
R1494 T1754 T1753 punct ",",astrocytes
R1495 T1755 T1757 nsubjpass which,determined
R1496 T1756 T1757 auxpass were,determined
R1497 T1757 T1753 relcl determined,astrocytes
R1498 T1758 T1757 agent by,determined
R1499 T1759 T1758 pobj immunoreactivity,by
R1500 T1760 T1757 prep for,determined
R1501 T1761 T1760 pobj GFAP,for
R1502 T1762 T1767 punct (,Biotechnology
R1503 T1763 T1767 amod sc-166481,Biotechnology
R1504 T1764 T1767 punct ",",Biotechnology
R1505 T1765 T1766 compound Santa,Cruz
R1506 T1766 T1767 compound Cruz,Biotechnology
R1507 T1767 T1761 appos Biotechnology,GFAP
R1508 T1768 T1767 punct ",",Biotechnology
R1509 T1769 T1767 conj CA,Biotechnology
R1510 T1770 T1769 punct ",",CA
R1511 T1771 T1767 meta USA,Biotechnology
R1512 T1772 T1767 punct ),Biotechnology
R1513 T1773 T1751 punct .,were
R1514 T1774 T1775 nsubj Cells,passaged
R1515 T1775 T1775 ROOT passaged,passaged
R1516 T1776 T1775 prep for,passaged
R1517 T1777 T1778 nummod 2-3,generations
R1518 T1778 T1776 pobj generations,for
R1519 T1779 T1780 auxpass were,used
R1520 T1780 T1775 conj used,passaged
R1521 T1781 T1780 prep in,used
R1522 T1782 T1784 det the,studies
R1523 T1783 T1784 amod following,studies
R1627 T1925 T1923 pobj Model,In
R1628 T1926 T1925 prep of,Model
R1629 T1927 T1926 pobj TBI,of
R1630 T1928 T1936 nsubjpass Established,performed
R1631 T1929 T1928 agent by,Established
R1632 T1930 T1934 compound Scratch,injury
R1633 T1931 T1932 compound Injury,Astrocyte
R1634 T1932 T1934 compound Astrocyte,injury
R1635 T1933 T1934 compound scratch,injury
R1636 T1934 T1929 pobj injury,by
R1637 T1935 T1936 auxpass was,performed
R1638 T1936 T1936 ROOT performed,performed
R1639 T1937 T1936 prep as,performed
R1640 T1938 T1937 prep in,as
R1641 T1939 T1941 det a,report
R1642 T1940 T1941 amod previous,report
R1643 T1941 T1938 pobj report,in
R1644 T1942 T1937 pobj [,as
R1645 T1943 T1942 nummod 7,[
R1646 T1944 T1942 conj ],[
R1647 T1945 T1936 punct .,performed
R1648 T1946 T1948 nsubjpass Astrocytes,planted
R1649 T1947 T1948 auxpass were,planted
R1650 T1948 T1948 ROOT planted,planted
R1651 T1949 T1948 prep in,planted
R1652 T1950 T1951 amod 6-well,plates
R1653 T1951 T1949 pobj plates,in
R1654 T1952 T1948 cc and,planted
R1655 T1953 T1948 conj grown,planted
R1656 T1954 T1953 prep to,grown
R1657 T1955 T1954 pobj confluence,to
R1658 T1956 T1948 punct .,planted
R1659 T1957 T1959 det The,monolayer
R1660 T1958 T1959 compound cell,monolayer
R1661 T1959 T1961 nsubjpass monolayer,scratched
R1662 T1960 T1961 auxpass was,scratched
R1663 T1961 T1961 ROOT scratched,scratched
R1664 T1962 T1961 prep with,scratched
R1665 T1963 T1967 det a,needle
R1666 T1964 T1967 amod sterile,needle
R1667 T1965 T1967 compound 26G,needle
R1668 T1966 T1967 compound syringe,needle
R1669 T1967 T1962 pobj needle,with
R1670 T1968 T1961 punct ",",scratched
R1671 T1969 T1961 advcl resulting,scratched
R1672 T1970 T1969 prep in,resulting
R1673 T1971 T1972 det the,formation
R1674 T1972 T1970 pobj formation,in
R1675 T1973 T1972 prep of,formation
R1676 T1974 T1978 det a,gap
R1677 T1975 T1976 nummod 0.5,mm
R1678 T1976 T1978 nmod mm,gap
R1679 T1977 T1978 amod wide,gap
R1680 T1978 T1973 pobj gap,of
R1681 T1979 T1961 punct .,scratched
R1682 T1980 T1981 advmod Immediately,after
R1683 T1981 T1986 prep after,washed
R1684 T1982 T1981 pobj scratch,after
R1685 T1983 T1986 punct ",",washed
R1686 T1984 T1986 nsubjpass cells,washed
R1687 T1985 T1986 auxpass were,washed
R1688 T1986 T1986 ROOT washed,washed
R1689 T1987 T1986 advmod twice,washed
R1690 T1988 T1986 prep with,washed
R1691 T1989 T1990 amod sterile,PBS
R1692 T1990 T1988 pobj PBS,with
R1693 T1991 T1986 punct ",",washed
R1694 T1992 T1986 conj cultured,washed
R1695 T1993 T1992 prep with,cultured
R1696 T1994 T1996 amod complete,medium
R1697 T1995 T1996 compound culture,medium
R1698 T1996 T1993 pobj medium,with
R1699 T1997 T1992 punct ",",cultured
R1700 T1998 T1992 cc and,cultured
R1701 T1999 T1992 conj named,cultured
R1702 T2000 T1999 prep as,named
R1703 T2001 T2002 compound TBI,group
R1704 T2002 T2000 pobj group,as
R1705 T2003 T1986 punct .,washed
R1706 T2004 T2013 nsubjpass Cells,used
R1707 T2005 T2004 punct ",",Cells
R1708 T2006 T2009 nsubjpass which,received
R1709 T2007 T2009 aux did,received
R1710 T2008 T2009 neg not,received
R1711 T2009 T2004 relcl received,Cells
R1712 T2010 T2009 dobj scratch,received
R1713 T2011 T2013 punct ",",used
R1714 T2012 T2013 auxpass were,used
R1715 T2013 T2013 ROOT used,used
R1716 T2014 T2013 prep as,used
R1717 T2015 T2016 amod blank,control
R1718 T2016 T2014 pobj control,as
R1719 T2017 T2013 cc and,used
R1720 T2018 T2013 conj named,used
R1721 T2019 T2018 prep as,named
R1722 T2020 T2021 amod sham,group
R1723 T2021 T2019 pobj group,as
R1724 T2022 T2013 punct .,used
R1844 T2171 T2171 ROOT 2.3,2.3
R1845 T2172 T2171 punct .,2.3
R1846 T2173 T2175 compound Cell,Measurement
R1847 T2174 T2175 compound Death,Measurement
R1848 T2175 T2184 nsubjpass Measurement,seeded
R1849 T2176 T2175 prep by,Measurement
R1850 T2177 T2178 compound CCK-8,Assay
R1851 T2178 T2176 pobj Assay,by
R1852 T2179 T2180 nummod 1,×
R1853 T2180 T2182 nummod ×,cells
R1854 T2181 T2182 amod 103/well,cells
R1855 T2182 T2184 nsubjpass cells,seeded
R1856 T2183 T2184 auxpass were,seeded
R1857 T2184 T2184 ROOT seeded,seeded
R1858 T2185 T2195 aux to,adhere
R1859 T2186 T2195 advmod 96-well,adhere
R1860 T2187 T2188 compound culture,plates
R1861 T2188 T2186 dobj plates,96-well
R1862 T2189 T2186 cc and,96-well
R1863 T2190 T2186 conj cultivated,96-well
R1864 T2191 T2195 mark for,adhere
R1865 T2192 T2193 nummod 24,h
R1866 T2193 T2195 nsubj h,adhere
R1867 T2194 T2195 aux to,adhere
R1868 T2195 T2184 xcomp adhere,seeded
R1869 T2196 T2184 punct .,seeded
R1870 T2197 T2201 advmod Then,scrated
R1871 T2198 T2199 det the,cell
R1872 T2199 T2201 nsubjpass cell,scrated
R1873 T2200 T2201 auxpass was,scrated
R1874 T2201 T2201 ROOT scrated,scrated
R1875 T2202 T2203 mark as,mentioned
R1876 T2203 T2201 advcl mentioned,scrated
R1877 T2204 T2203 advmod above,mentioned
R1878 T2205 T2201 punct .,scrated
R1879 T2206 T2208 compound Astrocyte,death
R1880 T2207 T2208 compound cell,death
R1881 T2208 T2210 nsubjpass death,assessed
R1882 T2209 T2210 auxpass was,assessed
R1883 T2210 T2210 ROOT assessed,assessed
R1884 T2211 T2210 agent by,assessed
R1885 T2212 T2211 pobj cell,by
R1886 T2213 T2210 advcl counting,assessed
R1887 T2214 T2216 nmod kit-8,CCK-8
R1888 T2215 T2216 punct (,CCK-8
R1889 T2216 T2223 nmod CCK-8,assay
R1890 T2217 T2216 punct ),CCK-8
R1891 T2218 T2223 punct (,assay
R1892 T2219 T2223 nmod Dojindo,assay
R1893 T2220 T2219 punct ",",Dojindo
R1894 T2221 T2223 nmod Japan,assay
R1895 T2222 T2223 punct ),assay
R1896 T2223 T2228 nsubj assay,according
R1897 T2224 T2225 nummod 24,h
R1898 T2225 T2226 npadvmod h,after
R1899 T2226 T2223 prep after,assay
R1900 T2227 T2226 pobj scratch,after
R1901 T2228 T2210 prep according,assessed
R1902 T2229 T2228 prep to,according
R1903 T2230 T2231 det the,manufacturer
R1904 T2231 T2233 poss manufacturer,protocol
R1905 T2232 T2231 case 's,manufacturer
R1906 T2233 T2229 pobj protocol,to
R1907 T2234 T2210 punct .,assessed
R1908 T2235 T2241 advmod Briefly,added
R1909 T2236 T2241 punct ",",added
R1910 T2237 T2239 nummod 10,CCK-8
R1911 T2238 T2239 compound μL,CCK-8
R1912 T2239 T2241 nsubjpass CCK-8,added
R1913 T2240 T2241 auxpass was,added
R1914 T2241 T2241 ROOT added,added
R1916 T2243 T2244 det every,well
R1917 T2244 T2242 pobj well,into
R1918 T2245 T2241 cc and,added
R1919 T2246 T2241 conj incubated,added
R1920 T2247 T2246 prep for,incubated
R1921 T2248 T2249 nummod 1,h
R1922 T2249 T2247 pobj h,for
R1923 T2250 T2241 punct .,added
R1924 T2251 T2255 advmod Then,read
R1925 T2252 T2253 compound OD,value
R1926 T2253 T2255 nsubjpass value,read
R1927 T2254 T2255 auxpass was,read
R1928 T2255 T2255 ROOT read,read
R1929 T2256 T2255 prep at,read
R1930 T2257 T2258 nummod 450,nm
R1931 T2258 T2256 pobj nm,at
R1932 T2259 T2255 advcl using,read
R1933 T2260 T2264 det a,reader
R1934 T2261 T2264 compound Bio-Rad,reader
R1935 T2262 T2264 compound ELISA,reader
R1936 T2263 T2264 compound microplate,reader
R1937 T2264 T2259 dobj reader,using
R1938 T2265 T2267 punct (,Laboratories
R1939 T2266 T2267 compound Bio-Rad,Laboratories
R1940 T2267 T2264 appos Laboratories,reader
R1941 T2268 T2267 punct ",",Laboratories
R1942 T2269 T2267 conj CA,Laboratories
R1943 T2270 T2269 punct ",",CA
R1944 T2271 T2269 conj USA,CA
R1945 T2272 T2267 punct ),Laboratories
R1946 T2273 T2255 punct .,read
R1947 T2274 T2275 det All,measurements
R1948 T2275 T2277 nsubjpass measurements,performed
R1949 T2276 T2277 auxpass were,performed
R1950 T2277 T2277 ROOT performed,performed
R1951 T2278 T2277 prep in,performed
R1952 T2279 T2278 pobj sextuplicate,in
R1953 T2280 T2277 punct .,performed
R1954 T2281 T2283 nsubjpass Results,expressed
R1955 T2282 T2283 auxpass were,expressed
R1956 T2283 T2283 ROOT expressed,expressed
R1957 T2284 T2283 prep as,expressed
R1958 T2285 T2284 pobj mean,as
R1959 T2286 T2285 prep of,mean
R1960 T2287 T2288 compound OD,value
R1961 T2288 T2286 pobj value,of
R1962 T2289 T2283 prep at,expressed
R1963 T2290 T2292 nummod 450,±
R1964 T2291 T2292 compound nm,±
R1965 T2292 T2293 compound ±,SD
R1966 T2293 T2289 pobj SD,at
R1967 T2294 T2283 punct .,expressed
R2313 T2674 T2674 ROOT 2.4,2.4
R2314 T2675 T2674 punct .,2.4
R2315 T2676 T2679 compound Electrophoretic,Assay
R2316 T2677 T2678 compound Mobility,Shift
R2317 T2678 T2679 compound Shift,Assay
R2318 T2679 T2687 nsubjpass Assay,washed
R2319 T2680 T2681 punct (,EMSA
R2320 T2681 T2679 appos EMSA,Assay
R2321 T2682 T2681 punct ),EMSA
R2322 T2683 T2679 appos Monolayers,Assay
R2323 T2684 T2683 prep of,Monolayers
R2324 T2685 T2684 pobj astrocytes,of
R2325 T2686 T2687 auxpass were,washed
R2326 T2687 T2687 ROOT washed,washed
R2327 T2688 T2687 prep with,washed
R2328 T2689 T2688 pobj PBS,with
R2329 T2690 T2687 cc and,washed
R2330 T2691 T2687 conj harvested,washed
R2331 T2692 T2691 prep by,harvested
R2332 T2693 T2692 pcomp scraping,by
R2333 T2694 T2693 prep into,scraping
R2334 T2695 T2696 amod cold,PBS
R2335 T2696 T2694 pobj PBS,into
R2336 T2697 T2687 punct .,washed
R2337 T2698 T2700 det The,pellet
R2338 T2699 T2700 compound cell,pellet
R2339 T2700 T2704 nsubj pellet,was
R2340 T2701 T2700 acl obtained,pellet
R2341 T2702 T2701 agent by,obtained
R2342 T2703 T2702 pobj centrifugation,by
R2343 T2704 T2705 auxpass was,resuspended
R2344 T2705 T2705 ROOT resuspended,resuspended
R2345 T2706 T2705 prep in,resuspended
R2346 T2707 T2706 pobj buffer,in
R2347 T2708 T2707 acl containing,buffer
R2348 T2709 T2711 nummod 10,HEPES
R2349 T2710 T2711 compound mM,HEPES
R2350 T2711 T2708 dobj HEPES,containing
R2351 T2712 T2713 punct (,pH
R2352 T2713 T2711 appos pH,HEPES
R2353 T2714 T2713 nummod 7.9,pH
R2359 T2720 T2719 punct ",",KCl
R2360 T2721 T2723 compound 0.1,EDTA
R2361 T2722 T2723 compound mM,EDTA
R2362 T2723 T2719 conj EDTA,KCl
R2363 T2724 T2723 punct ",",EDTA
R2364 T2725 T2727 nummod 0.1,EGTA
R2365 T2726 T2727 compound mM,EGTA
R2366 T2727 T2723 conj EGTA,EDTA
R2367 T2728 T2727 punct ",",EGTA
R2368 T2729 T2731 nummod 1.0,DTT
R2369 T2730 T2731 compound mM,DTT
R2370 T2731 T2727 conj DTT,EGTA
R2371 T2732 T2731 punct ",",DTT
R2372 T2733 T2731 cc and,DTT
R2373 T2734 T2737 nummod 0.5,fluoride
R2374 T2735 T2737 nmod mM,fluoride
R2375 T2736 T2737 compound phenylmethylsulfonyl,fluoride
R2376 T2737 T2731 conj fluoride,DTT
R2377 T2738 T2705 punct .,resuspended
R2378 T2739 T2745 advmod Then,added
R2379 T2740 T2741 nummod 10,%
R2380 T2741 T2743 compound %,P-40
R2381 T2742 T2743 compound Nonidet,P-40
R2382 T2743 T2745 nsubjpass P-40,added
R2383 T2744 T2745 auxpass was,added
R2384 T2745 T2745 ROOT added,added
R2385 T2746 T2745 cc and,added
R2386 T2747 T2745 conj vortexed,added
R2387 T2748 T2747 advmod briefly,vortexed
R2388 T2749 T2747 punct ",",vortexed
R2389 T2750 T2747 cc and,vortexed
R2390 T2751 T2752 det the,nuclei
R2391 T2752 T2754 nsubjpass nuclei,pelleted
R2392 T2753 T2754 auxpass were,pelleted
R2393 T2754 T2747 conj pelleted,vortexed
R2394 T2755 T2754 agent by,pelleted
R2395 T2756 T2755 pobj centrifugation,by
R2396 T2757 T2754 punct .,pelleted
R2397 T2758 T2760 det The,proteins
R2398 T2759 T2760 amod nuclear,proteins
R2399 T2760 T2762 nsubjpass proteins,extracted
R2400 T2761 T2762 auxpass were,extracted
R2401 T2762 T2762 ROOT extracted,extracted
R2402 T2763 T2762 prep with,extracted
R2403 T2764 T2763 pobj buffer,with
R2404 T2765 T2764 acl containing,buffer
R2405 T2766 T2768 nummod 20,HEPES
R2406 T2767 T2768 compound mM,HEPES
R2407 T2768 T2765 dobj HEPES,containing
R2408 T2769 T2770 punct (,pH
R2409 T2770 T2768 appos pH,HEPES
R2410 T2771 T2770 nummod 7.9,pH
R2411 T2772 T2770 punct ),pH
R2412 T2773 T2768 punct ",",HEPES
R2413 T2774 T2776 nummod 0.4,NaCl
R2414 T2775 T2776 compound mM,NaCl
R2415 T2776 T2768 appos NaCl,HEPES
R2416 T2777 T2776 punct ",",NaCl
R2417 T2778 T2780 nummod 1.0,EDTA
R2418 T2779 T2780 compound mM,EDTA
R2419 T2780 T2776 conj EDTA,NaCl
R2420 T2781 T2780 punct ",",EDTA
R2421 T2782 T2784 nummod 1.0,EGTA
R2422 T2783 T2784 compound mM,EGTA
R2423 T2784 T2780 conj EGTA,EDTA
R2424 T2785 T2784 punct ",",EGTA
R2425 T2786 T2788 nummod 1.0,DTT
R2452 T2813 T2814 auxpass was,stored
R2453 T2814 T2799 conj stored,removed
R2454 T2815 T2814 prep at,stored
R2455 T2816 T2818 nummod 80,C
R2456 T2817 T2818 nummod °,C
R2457 T2818 T2815 pobj C,at
R2458 T2819 T2814 prep until,stored
R2459 T2820 T2819 pobj use,until
R2460 T2821 T2814 punct .,stored
R2461 T2822 T2823 compound Protein,concentration
R2462 T2823 T2825 nsubjpass concentration,determined
R2463 T2824 T2825 auxpass was,determined
R2464 T2825 T2825 ROOT determined,determined
R2465 T2826 T2825 advcl using,determined
R2466 T2827 T2831 det a,kit
R2467 T2828 T2831 amod bicinchoninic,kit
R2468 T2829 T2830 compound acid,assay
R2469 T2830 T2831 compound assay,kit
R2470 T2831 T2826 dobj kit,using
R2471 T2832 T2831 prep with,kit
R2472 T2833 T2834 amod bovine,serum
R2473 T2834 T2832 pobj serum,with
R2474 T2835 T2831 amod albumin,kit
R2475 T2836 T2826 prep as,using
R2476 T2837 T2838 det the,standard
R2477 T2838 T2836 pobj standard,as
R2478 T2839 T2841 punct (,Biochemicals
R2479 T2840 T2841 compound Pierce,Biochemicals
R2480 T2841 T2826 parataxis Biochemicals,using
R2481 T2842 T2841 punct ",",Biochemicals
R2482 T2843 T2841 npadvmod Rockford,Biochemicals
R2483 T2844 T2843 punct ",",Rockford
R2484 T2845 T2843 conj IL,Rockford
R2485 T2846 T2845 punct ",",IL
R2486 T2847 T2843 npadvmod USA,Rockford
R2487 T2848 T2843 punct ),Rockford
R2488 T2849 T2841 punct .,Biochemicals
R2489 T2850 T2852 nsubjpass EMSA,performed
R2490 T2851 T2852 auxpass was,performed
R2491 T2852 T2852 ROOT performed,performed
R2492 T2853 T2852 advcl using,performed
R2493 T2854 T2855 compound gel,shift
R2494 T2855 T2857 compound shift,system
R2495 T2856 T2857 compound assay,system
R2496 T2857 T2853 dobj system,using
R2497 T2858 T2857 punct (,system
R2498 T2859 T2857 appos Promega,system
R2499 T2860 T2859 punct ",",Promega
R2500 T2861 T2859 conj Madison,Promega
R2501 T2862 T2861 punct ",",Madison
R2502 T2863 T2861 conj WI,Madison
R2503 T2864 T2863 punct ",",WI
R2518 T2879 T2879 ROOT end-labeled,end-labeled
R2519 T2880 T2879 agent by,end-labeled
R2520 T2881 T2882 compound T4-polynucleotide,kinase
R2521 T2882 T2880 pobj kinase,by
R2522 T2883 T2879 advcl using,end-labeled
R2523 T2884 T2888 compound [,ATP
R2524 T2885 T2886 compound γ-32P,]
R2525 T2886 T2888 compound ],ATP
R2526 T2887 T2888 punct -,ATP
R2527 T2888 T2883 dobj ATP,using
R2528 T2889 T2891 punct (,Biotech.
R2529 T2890 T2891 compound Free,Biotech.
R2530 T2891 T2888 appos Biotech.,ATP
R2531 T2892 T2891 punct ",",Biotech.
R2532 T2893 T2891 npadvmod Beijing,Biotech.
R2533 T2894 T2893 punct ",",Beijing
R2534 T2895 T2891 npadvmod China,Biotech.
R2535 T2896 T2891 punct ),Biotech.
R2536 T2897 T2879 punct .,end-labeled
R2537 T2898 T2899 compound Nuclear,protein
R2538 T2899 T2904 nsubj protein,was
R2539 T2900 T2899 punct (,protein
R2540 T2901 T2902 nummod 20,μg
R2541 T2902 T2899 appos μg,protein
R2542 T2903 T2899 punct ),protein
R2543 T2904 T2905 auxpass was,preincubated
R2544 T2905 T2905 ROOT preincubated,preincubated
R2545 T2906 T2905 prep in,preincubated
R2546 T2907 T2908 nummod 20,μL
R2547 T2908 T2910 nmod μL,buffer
R2548 T2909 T2910 amod binding,buffer
R2549 T2910 T2906 pobj buffer,in
R2550 T2911 T2910 acl containing,buffer
R2551 T2912 T2914 nummod 10,Tris-HCl
R2552 T2913 T2914 compound mM,Tris-HCl
R2553 T2914 T2911 dobj Tris-HCl,containing
R2554 T2915 T2916 punct (,PH
R2555 T2916 T2914 appos PH,Tris-HCl
R2556 T2917 T2916 nummod 7.5,PH
R2557 T2918 T2916 punct ),PH
R2558 T2919 T2914 punct ",",Tris-HCl
R2559 T2920 T2922 nummod 1,MgCl2
R2560 T2921 T2922 compound mM,MgCl2
R2561 T2922 T2914 conj MgCl2,Tris-HCl
R2562 T2923 T2922 punct ",",MgCl2
R2563 T2924 T2922 npadvmod 0.5,MgCl2
R2564 T2925 T2926 compound mM,NaCl
R2565 T2926 T2926 ROOT NaCl,NaCl
R2566 T2927 T2926 punct ",",NaCl
R2567 T2928 T2929 nummod 4,%
R2568 T2929 T2930 compound %,glycerol
R2569 T2930 T2926 appos glycerol,NaCl
R2570 T2931 T2930 punct ",",glycerol
R2571 T2932 T2934 nummod 0.5,EDTA
R2572 T2933 T2934 compound mM,EDTA
R2573 T2934 T2926 conj EDTA,NaCl
R2574 T2935 T2934 punct ",",EDTA
R2575 T2936 T2938 nummod 0.5,DTT
R2576 T2937 T2938 compound mM,DTT
R2577 T2938 T2934 conj DTT,EDTA
R2578 T2939 T2938 punct ",",DTT
R2579 T2940 T2938 cc and,DTT
R2580 T2941 T2942 nummod 2,μg
R2581 T2942 T2944 compound μg,dI-dC
R2582 T2943 T2944 compound poly,dI-dC
R2583 T2944 T2934 appos dI-dC,EDTA
R2584 T2945 T2944 prep for,dI-dC
R2585 T2946 T2947 nummod 20,minutes
R2586 T2947 T2945 pobj minutes,for
R2587 T2948 T2947 prep on,minutes
R2588 T2949 T2948 pobj ice,on
R2589 T2950 T2926 punct .,NaCl
R2590 T2951 T2964 prep After,continued
R2591 T2952 T2951 pobj addition,After
R2592 T2953 T2952 prep of,addition
R2593 T2954 T2959 det the,probe
R2594 T2955 T2956 nummod 1,μL
R2611 T2972 T2973 amod DNA-protein,complexes
R2612 T2973 T2975 nsubjpass complexes,separated
R2613 T2974 T2975 auxpass were,separated
R2614 T2975 T2975 ROOT separated,separated
R2615 T2976 T2975 agent by,separated
R2616 T2977 T2976 pobj electrophoresis,by
R2617 T2978 T2975 prep on,separated
R2618 T2979 T2980 nummod 4,%
R2619 T2980 T2983 nmod %,gel
R2620 T2981 T2983 amod nondenaturing,gel
R2621 T2982 T2983 compound polyacrylamide,gel
R2622 T2983 T2978 pobj gel,on
R2623 T2984 T2975 prep in,separated
R2624 T2985 T2988 nummod 0.5,buffer
R2625 T2986 T2988 nummod ×,buffer
R2626 T2987 T2988 compound TBE,buffer
R2627 T2988 T2984 pobj buffer,in
R2628 T2989 T2988 punct (,buffer
R2629 T2990 T2988 appos tris-borate-EDTA,buffer
R2630 T2991 T2988 punct ),buffer
R2631 T2992 T2975 prep at,separated
R2632 T2993 T2994 nummod 390,V
R2633 T2994 T2992 pobj V,at
R2634 T2995 T2975 prep for,separated
R2635 T2996 T2997 nummod 1,hour
R2636 T2997 T2995 pobj hour,for
R2637 T2998 T2975 prep at,separated
R2638 T2999 T3000 nummod 4,°
R2664 T3025 T3024 prep of,Levels
R2665 T3026 T3027 compound NF-κB,DNA
R2666 T3027 T3025 pobj DNA,of
R2667 T3028 T3029 amod binding,activity
R2668 T3029 T3025 pobj activity,of
R2669 T3030 T3031 auxpass were,quantified
R2670 T3031 T3031 ROOT quantified,quantified
R2671 T3032 T3031 agent by,quantified
R2672 T3033 T3034 compound software,ImageJ
R2673 T3034 T3032 pobj ImageJ,by
R2674 T3035 T3031 punct .,quantified
R2782 T3233 T3233 ROOT 2.5,2.5
R2783 T3234 T3233 punct .,2.5
R2784 T3235 T3237 compound RT-PCR,RNA
R2785 T3236 T3237 compound Total,RNA
R2786 T3237 T3238 nsubj RNA,was
R2787 T3238 T3238 ROOT was,was
R2788 T3239 T3238 acomp isolated,was
R2789 T3240 T3239 prep with,isolated
R2790 T3241 T3240 pobj Trizol,with
R2791 T3242 T3254 punct (,synthesized
R2792 T3243 T3252 nmod Invitrogen,cDNA
R2793 T3244 T3243 punct ",",Invitrogen
R2794 T3245 T3243 conj CA,Invitrogen
R2795 T3246 T3245 punct ",",CA
R2796 T3247 T3245 conj USA,CA
R2797 T3248 T3245 punct ),CA
R2798 T3249 T3245 punct ",",CA
R2799 T3250 T3245 cc and,CA
R2800 T3251 T3252 compound single-stranded,cDNA
R2801 T3252 T3254 nsubjpass cDNA,synthesized
R2802 T3253 T3254 auxpass was,synthesized
R2803 T3254 T3238 parataxis synthesized,was
R2804 T3255 T3254 prep from,synthesized
R2805 T3256 T3257 nummod 2,μg
R2806 T3257 T3255 pobj μg,from
R2807 T3258 T3257 prep of,μg
R2808 T3259 T3260 amod total,RNA
R2809 T3260 T3258 pobj RNA,of
R2810 T3261 T3257 prep with,μg
R2811 T3262 T3263 amod BU-Script,RT-Kit
R2812 T3263 T3261 pobj RT-Kit,with
R2813 T3264 T3265 punct (,Biunique
R2814 T3265 T3263 appos Biunique,RT-Kit
R2815 T3266 T3265 punct ",",Biunique
R2816 T3267 T3265 conj Jiangsu,Biunique
R2817 T3268 T3267 punct ",",Jiangsu
R2818 T3269 T3267 conj China,Jiangsu
R2819 T3270 T3254 punct ),synthesized
R2820 T3271 T3238 prep according,was
R2821 T3272 T3271 prep to,according
R2822 T3273 T3274 det the,manufacturer
R2823 T3274 T3276 poss manufacturer,protocol
R2824 T3275 T3274 case 's,manufacturer
R2825 T3276 T3272 pobj protocol,to
R2826 T3277 T3238 punct .,was
R2827 T3278 T3279 det The,cDNA
R2828 T3279 T3281 nsubjpass cDNA,stored
R2829 T3280 T3281 auxpass was,stored
R2830 T3281 T3281 ROOT stored,stored
R2831 T3282 T3281 prep in,stored
R2832 T3283 T3286 nummod −,C.
R2833 T3284 T3285 nummod 20,°
R2834 T3285 T3286 compound °,C.
R2835 T3286 T3282 pobj C.,in
R2836 T3287 T3288 amod Reverse,transcription
R2837 T3288 T3290 nsubjpass transcription,conducted
R2838 T3289 T3290 auxpass was,conducted
R2839 T3290 T3281 conj conducted,stored
R2840 T3291 T3290 prep with,conducted
R2841 T3292 T3295 compound GoTaq,Mix
R2842 T3293 T3295 compound Green,Mix
R2843 T3294 T3295 compound Master,Mix
R2844 T3295 T3291 pobj Mix,with
R2845 T3296 T3295 punct (,Mix
R2846 T3297 T3295 appos Promega,Mix
R2847 T3298 T3297 punct ",",Promega
R2848 T3299 T3297 conj WI,Promega
R2849 T3300 T3299 punct ",",WI
R2850 T3301 T3299 appos USA,WI
R2851 T3302 T3295 punct ),Mix
R2852 T3303 T3290 prep according,conducted
R2853 T3304 T3303 prep to,according
R2854 T3305 T3306 det the,manufacturer
R2855 T3306 T3308 poss manufacturer,protocol
R2856 T3307 T3306 case 's,manufacturer
R2857 T3308 T3304 pobj protocol,to
R2858 T3309 T3290 punct .,conducted
R2859 T3310 T3312 nsubj Table,shows
R2860 T3311 T3310 nummod 1,Table
R2861 T3312 T3312 ROOT shows,shows
R2862 T3313 T3314 det the,primers
R2863 T3314 T3312 dobj primers,shows
R2864 T3315 T3314 cc and,primers
R2865 T3316 T3317 compound PCR,parameters
R2866 T3317 T3314 conj parameters,primers
R2867 T3318 T3312 punct .,shows
R2868 T3319 T3320 compound PCR,products
R2869 T3320 T3322 nsubjpass products,detected
R2870 T3321 T3322 auxpass were,detected
R2871 T3322 T3322 ROOT detected,detected
R2872 T3323 T3322 agent by,detected
R2873 T3324 T3326 amod agarose,electrophoresis
R2874 T3325 T3326 compound gel,electrophoresis
R2875 T3326 T3323 pobj electrophoresis,by
R2876 T3327 T3322 punct .,detected
R2877 T3328 T3329 det The,intensity
R2878 T3329 T3334 nsubjpass intensity,analyzed
R2879 T3330 T3329 prep of,intensity
R2880 T3331 T3332 det the,bands
R2881 T3332 T3330 pobj bands,of
R2882 T3333 T3334 auxpass was,analyzed
R2883 T3334 T3334 ROOT analyzed,analyzed
R2884 T3335 T3334 agent by,analyzed
R2885 T3336 T3337 compound ImageJ,program
R2886 T3337 T3335 pobj program,by
R2887 T3338 T3334 punct .,analyzed
R2888 T3339 T3340 det The,level
R2889 T3340 T3344 nsubjpass level,used
R2890 T3341 T3340 prep of,level
R2891 T3342 T3341 pobj β-actin,of
R2892 T3343 T3344 auxpass was,used
R2893 T3344 T3344 ROOT used,used
R2894 T3345 T3344 prep as,used
R2895 T3346 T3348 det an,standard
R2896 T3347 T3348 amod internal,standard
R2897 T3348 T3345 pobj standard,as
R2898 T3349 T3344 punct .,used
R3191 T3697 T3697 ROOT 2.6,2.6
R3192 T3698 T3697 punct .,2.6
R3193 T3699 T3700 compound Western,Blot
R3194 T3700 T3702 nsubj Blot,obtain
R3195 T3701 T3702 aux To,obtain
R3196 T3702 T3702 ROOT obtain,obtain
R3197 T3703 T3704 amod total,protein
R3198 T3704 T3702 dobj protein,obtain
R3199 T3705 T3709 advmod lysates,homogenized
R3200 T3706 T3709 punct ",",homogenized
R3201 T3707 T3709 nsubjpass cells,homogenized
R3202 T3708 T3709 auxpass were,homogenized
R3203 T3709 T3702 advcl homogenized,obtain
R3204 T3710 T3709 prep in,homogenized
R3205 T3711 T3712 compound RIPA,buffer
R3206 T3712 T3710 pobj buffer,in
R3207 T3713 T3715 punct (,%
R3208 T3714 T3715 nummod 1,%
R3209 T3715 T3775 nsubjpass %,estimated
R3210 T3716 T3721 nummod NP40,deoxycholate
R3211 T3717 T3716 punct ",",NP40
R3212 T3718 T3719 nummod 0.5,%
R3213 T3719 T3721 nmod %,deoxycholate
R3214 T3720 T3721 compound sodium,deoxycholate
R3215 T3721 T3715 appos deoxycholate,%
R3216 T3722 T3721 punct ",",deoxycholate
R3217 T3723 T3724 nummod 0.1,%
R3218 T3724 T3715 appos %,%
R3219 T3725 T3715 npadvmod SDS,%
R3220 T3726 T3725 punct ",",SDS
R3221 T3727 T3729 nummod 1,EDTA
R3222 T3728 T3729 compound mM,EDTA
R3223 T3729 T3725 appos EDTA,SDS
R3224 T3730 T3725 punct ",",SDS
R3225 T3731 T3733 nummod 1,EGTA
R3226 T3732 T3733 compound mM,EGTA
R3227 T3733 T3725 appos EGTA,SDS
R3228 T3734 T3725 punct ",",SDS
R3229 T3735 T3737 nummod 1,Na3VO4
R3230 T3736 T3737 compound mM,Na3VO4
R3231 T3737 T3725 appos Na3VO4,SDS
R3232 T3738 T3725 punct ",",SDS
R3233 T3739 T3741 nummod 20,NaF
R3234 T3740 T3741 compound mM,NaF
R3235 T3741 T3725 appos NaF,SDS
R3236 T3742 T3725 punct ",",SDS
R3237 T3743 T3745 nummod 0.5,DTT
R3238 T3744 T3745 compound mM,DTT
R3239 T3745 T3725 appos DTT,SDS
R3240 T3746 T3725 punct ",",SDS
R3241 T3747 T3749 nummod 1,PMSF
R3242 T3748 T3749 compound mM,PMSF
R3243 T3749 T3725 appos PMSF,SDS
R3244 T3750 T3725 punct ",",SDS
R3245 T3751 T3715 cc and,%
R3246 T3752 T3753 compound protease,inhibitor
R3247 T3753 T3754 compound inhibitor,cocktail
R3248 T3754 T3715 conj cocktail,%
R3297 T3803 T3804 compound loading,buffer
R3298 T3804 T3800 pobj buffer,in
R3299 T3805 T3799 punct ",",heat-denatured
R3300 T3806 T3798 advcl separated,were
R3301 T3807 T3806 agent by,separated
R3302 T3808 T3809 nummod 10,%
R3303 T3809 T3815 compound %,electrophoresis
R3304 T3810 T3812 compound sodium,sulfate
R3305 T3811 T3812 compound dodecyl,sulfate
R3306 T3812 T3814 compound sulfate,gel
R3307 T3813 T3814 compound polyacrylamide,gel
R3308 T3814 T3815 compound gel,electrophoresis
R3309 T3815 T3807 pobj electrophoresis,by
R3310 T3816 T3806 punct ",",separated
R3311 T3817 T3806 cc and,separated
R3312 T3818 T3806 conj electroblotted,separated
R3313 T3819 T3818 prep onto,electroblotted
R3314 T3820 T3822 det a,membrane
R3315 T3821 T3822 amod nitrocellulose,membrane
R3316 T3822 T3819 pobj membrane,onto
R3317 T3823 T3798 punct .,were
R3318 T3824 T3829 prep For,blocked
R3319 T3825 T3824 pobj immunoblotting,For
R3320 T3826 T3829 punct ",",blocked
R3321 T3827 T3829 nsubjpass membranes,blocked
R3322 T3828 T3829 auxpass were,blocked
R3323 T3829 T3829 ROOT blocked,blocked
R3324 T3830 T3829 prep with,blocked
R3325 T3831 T3832 nummod 5,%
R3326 T3832 T3835 nmod %,milk
R3327 T3833 T3835 amod nonfat,milk
R3328 T3834 T3835 amod dry,milk
R3329 T3835 T3830 pobj milk,with
R3330 T3836 T3835 prep in,milk
R3331 T3837 T3838 compound saline,buffer
R3332 T3838 T3839 compound buffer,overnight
R3333 T3839 T3836 pobj overnight,in
R3355 T3861 T3860 punct ",",CA
R3356 T3862 T3860 conj USA,CA
R3357 T3863 T3860 punct ",",CA
R3358 T3864 T3865 nummod 43,kDa
R3359 T3865 T3860 appos kDa,CA
R3360 T3866 T3865 punct ),kDa
R3361 T3867 T3865 cc and,kDa
R3362 T3868 T3869 amod anti-matrix,metallopeptidase
R3363 T3869 T3865 conj metallopeptidase,kDa
R3364 T3870 T3869 nummod 9,metallopeptidase
R3365 T3871 T3872 punct (,MMP9
R3366 T3872 T3869 appos MMP9,metallopeptidase
R3367 T3873 T3872 punct ),MMP9
R3368 T3874 T3875 punct (,sc-6841
R3369 T3875 T3869 appos sc-6841,metallopeptidase
R3370 T3876 T3875 punct ",",sc-6841
R3371 T3877 T3879 compound Santa,Biotechnology
R3372 T3878 T3879 compound Cruz,Biotechnology
R3373 T3879 T3875 appos Biotechnology,sc-6841
R3374 T3880 T3879 punct ",",Biotechnology
R3375 T3881 T3879 conj CA,Biotechnology
R3376 T3882 T3881 punct ",",CA
R3377 T3883 T3881 conj USA,CA
R3378 T3884 T3881 punct ",",CA
R3379 T3885 T3886 nummod 92,kDa
R3380 T3886 T3881 appos kDa,CA
R3381 T3887 T3875 punct ),sc-6841
R3382 T3888 T3854 punct .,sc-130657
R3383 T3889 T3891 det Each,antibody
R3384 T3890 T3891 amod primary,antibody
R3385 T3891 T3893 nsubjpass antibody,diluted
R3386 T3892 T3893 auxpass was,diluted
R3387 T3893 T3893 ROOT diluted,diluted
R3388 T3894 T3893 advmod appropriately,diluted
R3389 T3895 T3893 prep in,diluted
R3390 T3896 T3895 pcomp blocking,in
R3391 T3897 T3896 dobj buffer,blocking
R3392 T3898 T3893 cc and,diluted
R3393 T3899 T3900 advmod then,added
R3394 T3900 T3893 conj added,diluted
R3395 T3901 T3900 prep to,added
R3396 T3902 T3903 det the,blots
R3397 T3903 T3901 pobj blots,to
R3398 T3904 T3900 prep for,added
R3399 T3905 T3906 nummod 1,h
R3400 T3906 T3904 pobj h,for
R3401 T3907 T3906 prep at,h
R3402 T3908 T3909 compound room,temperature
R3403 T3909 T3907 pobj temperature,at
R3404 T3910 T3900 punct .,added
R3405 T3911 T3912 det The,blots
R3406 T3912 T3914 nsubjpass blots,washed
R3407 T3913 T3914 auxpass were,washed
R3408 T3914 T3914 ROOT washed,washed
R3409 T3915 T3916 nummod three,times
R3410 T3916 T3914 npadvmod times,washed
R3433 T3939 T3940 auxpass were,incubated
R3434 T3940 T3914 conj incubated,washed
R3435 T3941 T3940 prep with,incubated
R3436 T3942 T3943 amod enhanced,chemiluminescence
R3437 T3943 T3941 pobj chemiluminescence,with
R3438 T3944 T3945 punct (,ECL
R3439 T3945 T3943 appos ECL,chemiluminescence
R3440 T3946 T3945 punct ),ECL
R3441 T3947 T3948 compound detection,system
R3442 T3948 T3943 conj system,chemiluminescence
R3443 T3949 T3948 punct (,system
R3444 T3950 T3951 compound Amersham,Biosciences
R3445 T3951 T3948 appos Biosciences,system
R3446 T3952 T3951 punct ",",Biosciences
R3447 T3953 T3951 conj Bucks,Biosciences
R3448 T3954 T3953 punct ",",Bucks
R3449 T3955 T3953 conj UK,Bucks
R3450 T3956 T3953 punct ),Bucks
R3451 T3957 T3940 cc and,incubated
R3452 T3958 T3940 conj exposed,incubated
R3453 T3959 T3958 prep to,exposed
R3454 T3960 T3961 amod radiographic,film
R3455 T3961 T3959 pobj film,to
R3456 T3962 T3964 punct (,Hyperfilm
R3457 T3963 T3964 compound Fuji,Hyperfilm
R3458 T3964 T3961 appos Hyperfilm,film
R3459 T3965 T3964 punct ",",Hyperfilm
R3460 T3966 T3964 conj Tokyo,Hyperfilm
R3461 T3967 T3966 punct ",",Tokyo
R3462 T3968 T3966 conj Japan,Tokyo
R3463 T3969 T3964 punct ),Hyperfilm
R3464 T3970 T3940 punct .,incubated
R3465 T3971 T3973 nsubjpass ImageJ,used
R3466 T3972 T3973 auxpass was,used
R3467 T3973 T3973 ROOT used,used
R3468 T3974 T3975 aux to,analyze
R3469 T3975 T3973 xcomp analyze,used
R3470 T3976 T3977 det the,intensity
R3471 T3977 T3975 dobj intensity,analyze
R3472 T3978 T3977 prep of,intensity
R3473 T3979 T3980 det the,blots
R3474 T3980 T3978 pobj blots,of
R3475 T3981 T3973 punct .,used
R3476 T3982 T3983 det The,level
R3477 T3983 T3987 nsubjpass level,used
R3478 T3984 T3983 prep of,level
R3479 T3985 T3984 pobj β-actin,of
R3480 T3986 T3987 auxpass was,used
R3481 T3987 T3987 ROOT used,used
R3482 T3988 T3987 prep as,used
R3483 T3989 T3990 amod internal,standard
R3484 T3990 T3988 pobj standard,as
R3485 T3991 T3987 punct .,used
R3873 T4560 T4560 ROOT 2.8,2.8
R3874 T4561 T4560 punct .,2.8
R3875 T4562 T4564 compound Gelatine,Cells
R3887 T4574 T4576 nummod 50,Tris-HCl
R3888 T4575 T4576 compound mM,Tris-HCl
R3889 T4576 T4573 dobj Tris-HCl,containing
R3890 T4577 T4578 punct (,pH
R3891 T4578 T4576 appos pH,Tris-HCl
R3892 T4579 T4578 nummod 7.4,pH
R3893 T4580 T4578 punct ),pH
R3894 T4581 T4576 punct ",",Tris-HCl
R3895 T4582 T4584 nummod 150,NaCl
R3896 T4583 T4584 compound mM,NaCl
R3897 T4584 T4576 conj NaCl,Tris-HCl
R3898 T4585 T4584 punct ",",NaCl
R3899 T4586 T4588 nummod 5,CaCl2
R3900 T4587 T4588 compound mM,CaCl2
R3901 T4588 T4584 conj CaCl2,NaCl
R3902 T4589 T4588 punct ",",CaCl2
R3903 T4590 T4592 nummod 0.2,NaN3
R3904 T4591 T4592 compound mM,NaN3
R3905 T4592 T4588 conj NaN3,CaCl2
R3906 T4593 T4592 punct ",",NaN3
R3907 T4594 T4592 cc and,NaN3
R3908 T4595 T4596 nummod 0.01,%
R3909 T4596 T4597 compound %,Triton
R3910 T4597 T4592 conj Triton,NaN3
R3911 T4598 T4569 punct .,homogenized
R3912 T4599 T4600 amod Soluble,extracts
R3913 T4600 T4602 nsubjpass extracts,separated
R3914 T4601 T4602 auxpass were,separated
R3915 T4602 T4602 ROOT separated,separated
R3916 T4603 T4602 agent by,separated
R3917 T4604 T4603 pobj centrifugation,by
R3918 T4605 T4602 cc and,separated
R3919 T4606 T4614 nsubjpass stored,performed
R3920 T4607 T4606 prep at,stored
R3921 T4608 T4609 nummod 20,°
R3922 T4609 T4612 compound °,zymography
R3923 T4610 T4612 compound C.,zymography
R3924 T4611 T4612 compound Gelatin,zymography
R3925 T4612 T4607 pobj zymography,at
R3926 T4613 T4614 auxpass was,performed
R3927 T4614 T4602 conj performed,separated
R3928 T4615 T4614 xcomp according,performed
R3929 T4616 T4615 prep to,according
R3930 T4617 T4618 det the,manufacturer
R3931 T4618 T4620 poss manufacturer,instructions
R3932 T4619 T4618 case 's,manufacturer
R3933 T4620 T4616 pobj instructions,to
R3934 T4621 T4624 punct (,Inc
R3935 T4622 T4624 compound Genmed,Inc
R3936 T4623 T4624 compound Scientifics,Inc
R3937 T4624 T4620 appos Inc,instructions
R3938 T4625 T4624 punct ",",Inc
R3939 T4626 T4624 conj MA,Inc
R3940 T4627 T4626 punct ",",MA
R3941 T4628 T4626 appos USA,MA
R3942 T4629 T4626 punct ),MA
R3943 T4630 T4614 punct .,performed
R3944 T4631 T4639 advmod Briefly,separated
R3945 T4632 T4639 punct ",",separated
R3946 T4633 T4637 nummod 40,extracts
R3947 T4634 T4637 compound μg,extracts
R3948 T4635 T4636 compound cytosolic,protein
R3949 T4636 T4637 compound protein,extracts
R3950 T4637 T4639 nsubjpass extracts,separated
R3951 T4638 T4639 auxpass were,separated
R3952 T4639 T4639 ROOT separated,separated
R3953 T4640 T4639 prep by,separated
R3954 T4641 T4640 pobj electrophoresis,by
R3955 T4642 T4639 punct .,separated
R3956 T4643 T4647 advmod Then,renatured
R3957 T4644 T4645 det the,proteins
R3958 T4645 T4647 nsubjpass proteins,renatured
R3959 T4646 T4647 auxpass were,renatured
R3960 T4647 T4647 ROOT renatured,renatured
R3961 T4648 T4647 agent by,renatured
R3962 T4649 T4648 pobj incubation,by
R3963 T4650 T4647 prep in,renatured
R3975 T4662 T4663 nummod 40,h
R3976 T4663 T4661 pobj h,for
R3977 T4664 T4657 prep at,incubated
R3978 T4665 T4666 nummod 37,°
R3979 T4666 T4664 pobj °,at
R3980 T4667 T4657 conj C,incubated
R3981 T4668 T4669 aux to,enable
R3982 T4669 T4657 advcl enable,incubated
R3983 T4670 T4671 det the,MMP9
R3984 T4671 T4669 dobj MMP9,enable
R3985 T4672 T4673 aux to,cleave
R3986 T4673 T4669 xcomp cleave,enable
R3987 T4674 T4675 det the,gelatin
R3988 T4675 T4673 dobj gelatin,cleave
R3989 T4676 T4647 punct .,renatured
R3990 T4677 T4685 prep After,stained
R3991 T4678 T4677 pcomp rinsing,After
R3992 T4679 T4678 prep in,rinsing
R3993 T4680 T4679 pobj water,in
R3994 T4681 T4685 punct ",",stained
R3995 T4682 T4683 det each,gel
R3996 T4683 T4685 nsubjpass gel,stained
R3997 T4684 T4685 auxpass was,stained
R3998 T4685 T4685 ROOT stained,stained
R3999 T4686 T4685 prep with,stained
R4000 T4687 T4688 compound Coomassie,blue
R4001 T4688 T4686 pobj blue,with
R4002 T4689 T4688 prep for,blue
R4003 T4690 T4691 nummod 1,h
R4004 T4691 T4689 pobj h,for
R4005 T4692 T4691 cc and,h
R4006 T4693 T4688 conj destained,blue
R4007 T4694 T4693 prep in,destained
R4008 T4695 T4696 nummod 50,%
R4009 T4696 T4697 compound %,methanol
R4010 T4697 T4694 pobj methanol,in
R4011 T4698 T4685 punct .,stained
R4012 T4699 T4700 amod Proteolytic,activities
R4013 T4700 T4702 nsubjpass activities,showed
R4014 T4701 T4702 auxpass were,showed
R4015 T4702 T4702 ROOT showed,showed
R4016 T4703 T4702 agent by,showed
R4017 T4704 T4705 amod clear,bands
R4018 T4705 T4703 pobj bands,by
R4019 T4706 T4705 prep in,bands
R4020 T4707 T4708 amod blue,gel
R4021 T4708 T4706 pobj gel,in
R4022 T4709 T4710 nsubj which,indicates
R4023 T4710 T4702 advcl indicates,showed
R4024 T4711 T4712 det the,lysis
R4025 T4712 T4710 dobj lysis,indicates
R4026 T4713 T4712 prep of,lysis
R4027 T4714 T4715 det the,substrate
R4028 T4715 T4713 pobj substrate,of
R4029 T4716 T4702 punct .,showed
R4030 T4717 T4723 nsubjpass Quantification,performed
R4031 T4718 T4717 prep of,Quantification
R4082 T4799 T4799 ROOT 2.9,2.9
R4083 T4800 T4799 punct .,2.9
R4084 T4801 T4802 compound Statistical,Analysis
R4085 T4802 T4803 compound Analysis,Data
R4086 T4803 T4805 nsubjpass Data,expressed
R4087 T4804 T4805 auxpass were,expressed
R4088 T4805 T4805 ROOT expressed,expressed
R4089 T4806 T4811 mark as,evaluated
R4090 T4807 T4809 amod mean,SD
R4091 T4808 T4809 compound ±,SD
R4092 T4809 T4811 nsubj SD,evaluated
R4093 T4810 T4809 cc and,SD
R4094 T4811 T4805 advcl evaluated,expressed
R4095 T4812 T4811 agent by,evaluated
R4096 T4813 T4812 pobj ANOVA,by
R4097 T4814 T4813 cc and,ANOVA
R4098 T4815 T4813 conj LSD,ANOVA
R4099 T4816 T4818 amod multiple,test
R4100 T4817 T4818 compound comparison,test
R4101 T4818 T4811 dobj test,evaluated
R4102 T4819 T4805 punct .,expressed
R4103 T4820 T4821 compound P,values
R4104 T4821 T4822 nsubj values,<
R4105 T4822 T4825 dep <,considered
R4106 T4823 T4825 nsubjpass 0.05,considered
R4107 T4824 T4825 auxpass were,considered
R4108 T4825 T4825 ROOT considered,considered
R4109 T4826 T4827 aux to,be
R4110 T4827 T4825 xcomp be,considered
R4111 T4828 T4827 acomp significant,be
R4112 T4829 T4825 punct .,considered
R4113 T4830 T4831 det All,analyses
R4114 T4831 T4833 nsubjpass analyses,performed
R4115 T4832 T4833 auxpass were,performed
R4116 T4833 T4833 ROOT performed,performed
R4117 T4834 T4833 prep by,performed
R4118 T4835 T4834 pcomp using,by
R4119 T4836 T4838 nmod SPSS,software
R4120 T4837 T4836 nummod 18.0,SPSS
R4121 T4838 T4835 dobj software,using
R4122 T4839 T4833 punct .,performed
R1249 T1509 T1510 amod Primary,Culture
R1250 T1510 T1522 nsubjpass Culture,provided
R1251 T1511 T1510 cc and,Culture
R1252 T1512 T1510 conj Identification,Culture
R1254 T1514 T1516 compound Mouse,Nrf2
R1255 T1515 T1516 compound Astrocytes,Nrf2
R1256 T1516 T1518 compound Nrf2,ICR
R1257 T1517 T1518 compound knockout,ICR
R1258 T1518 T1519 compound ICR,mice
R1259 T1519 T1513 pobj mice,of
R1260 T1520 T1522 auxpass were,provided
R1261 T1521 T1522 advmod kindly,provided
R1262 T1522 T1522 ROOT provided,provided
R1263 T1523 T1522 agent by,provided
R1264 T1524 T1527 compound Dr.,Kensler
R1265 T1525 T1527 compound Thomas,Kensler
R1266 T1526 T1527 compound W.,Kensler
R1267 T1527 T1523 pobj Kensler,by
R1268 T1528 T1527 punct (,Kensler
R1269 T1529 T1530 compound Johns,Hopkins
R1270 T1530 T1531 compound Hopkins,University
R1271 T1531 T1527 appos University,Kensler
R1272 T1532 T1531 punct ",",University
R1273 T1533 T1531 conj Baltimore,University
R1274 T1534 T1533 punct ",",Baltimore
R1275 T1535 T1533 conj MD,Baltimore
R1276 T1536 T1535 punct ",",MD
R1277 T1537 T1535 conj USA,MD
R1278 T1538 T1527 punct ),Kensler
R1279 T1539 T1522 punct .,provided
R1280 T1540 T1541 amod Primary,astrocytes
R1281 T1541 T1543 nsubjpass astrocytes,obtained
R1282 T1542 T1543 auxpass were,obtained
R1283 T1543 T1543 ROOT obtained,obtained
R1284 T1544 T1543 prep from,obtained
R1285 T1545 T1549 amod postnatal,type
R1286 T1546 T1549 amod 2-day-old,type
R1300 T1560 T1561 nummod 6,mice
R1301 T1561 T1558 appos mice,mice
R1302 T1562 T1561 prep for,mice
R1303 T1563 T1564 det each,genotype
R1304 T1564 T1562 pobj genotype,for
R1305 T1565 T1558 punct ),mice
R1306 T1566 T1543 punct .,obtained
R1307 T1567 T1573 prep Following,dissected
R1308 T1568 T1567 pobj decapitation,Following
R1309 T1569 T1573 punct ",",dissected
R1377 T1637 T1634 prep with,dissociation
R1378 T1638 T1639 nummod 20,%
R1379 T1639 T1640 compound %,FBS
R1380 T1640 T1637 pobj FBS,with
R1381 T1641 T1640 cc and,FBS
R1382 T1642 T1644 nummod 5,penicillin
R1383 T1643 T1644 amod units/mL,penicillin
R1384 T1644 T1640 conj penicillin,FBS
R1385 T1645 T1644 punct ",",penicillin
R1454 T1714 T1715 nummod 7,days
R1455 T1715 T1712 pobj days,for
R1456 T1716 T1717 aux to,reach
R1457 T1717 T1711 advcl reach,expanded
R1458 T1718 T1717 dobj confluence,reach
R1459 T1719 T1711 punct .,expanded
R1460 T1720 T1726 advmod Then,shaken
R1524 T1784 T1781 pobj studies,in
R1525 T1785 T1775 punct .,passaged
R1623 T1921 T1921 ROOT 2.2,2.2
R1624 T1922 T1921 punct .,2.2
R1625 T1923 T1936 prep In,performed
R1626 T1924 T1925 compound Vitro,Model
R3249 T3755 T3715 prep in,%
R3250 T3756 T3755 pobj PBS,in
R3251 T3757 T3758 quantmod pH,7.4
R3252 T3758 T3715 dep 7.4,%
R3253 T3759 T3758 punct ),7.4
R3254 T3760 T3715 cc and,%
R3255 T3761 T3715 conj centrifuged,%
R3256 T3762 T3761 prep at,centrifuged
R3257 T3763 T3764 nummod "12,000",g
R3258 T3764 T3762 pobj g,at
R3259 T3765 T3761 prep for,centrifuged
R3260 T3766 T3767 nummod 15,min
R3261 T3767 T3765 pobj min,for
R3262 T3768 T3767 prep at,min
R3263 T3769 T3770 nummod 4,°
R3264 T3770 T3773 nummod °,concentrations
R3265 T3771 T3773 nmod C.,concentrations
R3266 T3772 T3773 compound Protein,concentrations
R3267 T3773 T3768 pobj concentrations,at
R3268 T3774 T3775 auxpass were,estimated
R3269 T3775 T3702 conj estimated,obtain
R3270 T3776 T3775 agent by,estimated
R3271 T3777 T3781 compound Coomassie,Reagent
R3272 T3778 T3779 compound Plus,Protein
R3273 T3779 T3781 compound Protein,Reagent
R3274 T3780 T3781 compound Assay,Reagent
R3275 T3781 T3776 pobj Reagent,by
R3276 T3782 T3783 punct (,Pierce
R3277 T3783 T3781 appos Pierce,Reagent
R3278 T3784 T3783 punct ",",Pierce
R3279 T3785 T3783 prep IL,Pierce
R3280 T3786 T3785 punct ",",IL
R3281 T3787 T3785 npadvmod USA,IL
R3282 T3788 T3783 punct ),Pierce
R3283 T3789 T3775 punct .,estimated
R3284 T3790 T3791 nummod Fifty,micrograms
R3285 T3791 T3798 nsubj micrograms,were
R3286 T3792 T3791 prep of,micrograms
R3287 T3793 T3797 det the,extracts
R3288 T3794 T3797 amod resulting,extracts
R3289 T3795 T3797 amod cytosolic,extracts
R3290 T3796 T3797 compound protein,extracts
R3291 T3797 T3792 pobj extracts,of
R3292 T3798 T3798 ROOT were,were
R3293 T3799 T3798 acomp heat-denatured,were
R3294 T3800 T3799 prep in,heat-denatured
R3295 T3801 T3804 compound Laemmli,buffer
R3296 T3802 T3804 compound sample,buffer
R3334 T3840 T3829 prep at,blocked
R3335 T3841 T3842 nummod 4,°
R3336 T3842 T3843 nummod °,C
R3337 T3843 T3840 pobj C,at
R3338 T3844 T3829 punct ",",blocked
R3339 T3845 T3829 cc and,blocked
R3340 T3846 T3848 det the,antibodies
R3341 T3847 T3848 amod following,antibodies
R3342 T3848 T3850 nsubjpass antibodies,used
R3343 T3849 T3850 auxpass were,used
R3344 T3850 T3829 conj used,blocked
R3345 T3851 T3852 punct :,anti-β-actin
R3346 T3852 T3850 prep anti-β-actin,used
R3347 T3853 T3854 punct (,sc-130657
R3348 T3854 T3852 dep sc-130657,anti-β-actin
R3349 T3855 T3854 punct ",",sc-130657
R3350 T3856 T3858 compound Santa,Biotechnology
R3351 T3857 T3858 compound Cruz,Biotechnology
R3352 T3858 T3854 conj Biotechnology,sc-130657
R3353 T3859 T3858 punct ",",Biotechnology
R3354 T3860 T3858 conj CA,Biotechnology
R3411 T3917 T3914 prep in,washed
R3412 T3918 T3920 det the,buffer
R3413 T3919 T3920 amod washing,buffer
R3414 T3920 T3917 pobj buffer,in
R3415 T3921 T3914 cc and,washed
R3416 T3922 T3914 conj covered,washed
R3417 T3923 T3922 prep with,covered
R3418 T3924 T3928 det the,antibody
R3419 T3925 T3928 amod horseradish,antibody
R3420 T3926 T3928 amod peroxidase-linked,antibody
R3421 T3927 T3928 amod secondary,antibody
R3422 T3928 T3923 pobj antibody,with
R3423 T3929 T3922 prep at,covered
R3424 T3930 T3931 det a,1
R3425 T3931 T3929 pobj 1,at
R3426 T3932 T3934 punct :,dilution
R3427 T3933 T3934 nummod 2000,dilution
R3428 T3934 T3940 nsubjpass dilution,incubated
R3429 T3935 T3934 prep for,dilution
R3430 T3936 T3938 nummod 1,Blots
R3431 T3937 T3938 compound h.,Blots
R3432 T3938 T3935 pobj Blots,for
R3876 T4563 T4564 compound Zymography,Cells
R3877 T4564 T4569 nsubjpass Cells,homogenized
R3878 T4565 T4564 prep of,Cells
R3879 T4566 T4567 nummod four,groups
R3880 T4567 T4565 pobj groups,of
R3881 T4568 T4569 auxpass were,homogenized
R3882 T4569 T4569 ROOT homogenized,homogenized
R3883 T4570 T4569 prep in,homogenized
R3884 T4571 T4572 compound lysis,buffer
R3885 T4572 T4570 pobj buffer,in
R3886 T4573 T4572 acl containing,buffer
R3964 T4651 T4652 nummod 2.5,%
R3965 T4652 T4654 compound %,X-100
R3966 T4653 T4654 compound Triton,X-100
R3967 T4654 T4650 pobj X-100,in
R3968 T4655 T4647 cc and,renatured
R3969 T4656 T4657 advmod then,incubated
R3970 T4657 T4647 conj incubated,renatured
R3971 T4658 T4657 prep in,incubated
R3972 T4659 T4660 compound substrate,buffer
R3973 T4660 T4658 pobj buffer,in
R3974 T4661 T4660 prep for,buffer
R4032 T4719 T4721 compound MMP9,density
R4033 T4720 T4721 compound band,density
R4034 T4721 T4718 pobj density,of
R4035 T4722 T4723 auxpass was,performed
R4036 T4723 T4723 ROOT performed,performed
R4037 T4724 T4723 prep with,performed
R4038 T4725 T4726 compound image,analysis
R4039 T4726 T4727 compound analysis,program
R4040 T4727 T4728 compound program,ImageJ
R4041 T4728 T4724 pobj ImageJ,with
R4042 T4729 T4723 punct .,performed
R2354 T2715 T2713 punct ),pH
R2355 T2716 T2711 punct ",",HEPES
R2356 T2717 T2719 nummod 10,KCl
R2357 T2718 T2719 compound mM,KCl
R2358 T2719 T2711 conj KCl,HEPES
R2426 T2787 T2788 compound mM,DTT
R2427 T2788 T2784 conj DTT,EGTA
R2428 T2789 T2788 punct ",",DTT
R2429 T2790 T2788 cc and,DTT
R2430 T2791 T2794 nummod 1.0,fluoride
R2431 T2792 T2794 compound mM,fluoride
R2432 T2793 T2794 compound phenylmethylsulfonyl,fluoride
R2433 T2794 T2788 conj fluoride,DTT
R2434 T2795 T2762 punct .,extracted
R2435 T2796 T2797 amod Insoluble,material
R2436 T2797 T2799 nsubjpass material,removed
R2437 T2798 T2799 auxpass was,removed
R2438 T2799 T2799 ROOT removed,removed
R2439 T2800 T2799 agent by,removed
R2440 T2801 T2800 pobj centrifugation,by
R2441 T2802 T2799 prep at,removed
R2442 T2803 T2804 nummod 14000,rpm
R2443 T2804 T2802 pobj rpm,at
R2444 T2805 T2799 punct ",",removed
R2445 T2806 T2799 cc and,removed
R2446 T2807 T2808 det the,supernatant
R2447 T2808 T2814 nsubjpass supernatant,stored
R2448 T2809 T2808 acl containing,supernatant
R2449 T2810 T2812 det the,proteins
R2450 T2811 T2812 amod nuclear,proteins
R2451 T2812 T2809 dobj proteins,containing
R2504 T2865 T2863 conj USA,WI
R2505 T2866 T2857 punct ),system
R2506 T2867 T2852 punct .,performed
R2507 T2868 T2870 compound Consensus,probe
R2508 T2869 T2870 compound oligonucleotide,probe
R2509 T2870 T2879 nsubjpass probe,end-labeled
R2510 T2871 T2870 punct (,probe
R2511 T2872 T2875 nummod 5,AGTTGAGGGGACTTTCCCAGGG-3
R2512 T2873 T2875 punct ′,AGTTGAGGGGACTTTCCCAGGG-3
R2513 T2874 T2875 punct -,AGTTGAGGGGACTTTCCCAGGG-3
R2514 T2875 T2870 appos AGTTGAGGGGACTTTCCCAGGG-3,probe
R2515 T2876 T2875 appos ′,AGTTGAGGGGACTTTCCCAGGG-3
R2516 T2877 T2875 punct ),AGTTGAGGGGACTTTCCCAGGG-3
R2517 T2878 T2879 auxpass was,end-labeled
R2595 T2956 T2959 nmod μL,probe
R2596 T2957 T2959 amod 32P-labled,probe
R2597 T2958 T2959 compound oligonucleotide,probe
R2598 T2959 T2953 pobj probe,of
R2599 T2960 T2964 punct ",",continued
R2600 T2961 T2962 det the,incubation
R2601 T2962 T2964 nsubjpass incubation,continued
R2602 T2963 T2964 auxpass was,continued
R2603 T2964 T2964 ROOT continued,continued
R2604 T2965 T2964 prep for,continued
R2605 T2966 T2967 nummod 20,minutes
R2606 T2967 T2965 pobj minutes,for
R2607 T2968 T2967 prep on,minutes
R2608 T2969 T2968 pobj ice,on
R2609 T2970 T2964 punct .,continued
R2610 T2971 T2973 det The,complexes
R2639 T3000 T2998 pobj °,at
R2640 T3001 T2975 npadvmod C,separated
R2641 T3002 T2975 punct .,separated
R2642 T3003 T3009 prep After,dried
R2643 T3004 T3003 pobj electrophoresis,After
R2644 T3005 T3009 punct ",",dried
R2645 T3006 T3007 det the,gel
R2646 T3007 T3009 nsubjpass gel,dried
R2647 T3008 T3009 auxpass was,dried
R2648 T3009 T3009 ROOT dried,dried
R2649 T3010 T3009 cc and,dried
R2650 T3011 T3009 conj exposed,dried
R2651 T3012 T3011 prep to,exposed
R2652 T3013 T3014 compound X-ray,film
R2653 T3014 T3012 pobj film,to
R2654 T3015 T3017 punct (,Hyperfilm
R2655 T3016 T3017 compound Fuji,Hyperfilm
R2656 T3017 T3014 appos Hyperfilm,film
R2657 T3018 T3017 punct ",",Hyperfilm
R2658 T3019 T3017 npadvmod Tokyo,Hyperfilm
R2659 T3020 T3019 punct ",",Tokyo
R2660 T3021 T3019 conj Japan,Tokyo
R2661 T3022 T3017 punct ),Hyperfilm
R2662 T3023 T3009 punct .,dried
R2663 T3024 T3031 nsubjpass Levels,quantified
R1915 T2242 T2241 prep into,added

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T4314 7542-7545 Protein denotes Rad
T4313 7262-7265 Protein denotes HRP
T3693 5858-5888 Protein denotes anti-matrix metallopeptidase 9
T3692 5793-5798 Protein denotes actin
T2673 4352-4359 Binding denotes binding
T2672 4342-4347 Protein denotes NF-κB
T2671 3433-3453 Protein denotes bovine serum albumin
T4312 7249-7261 Protein denotes streptavidin
T4311 6743-6749 Gene_expression denotes Levels
T4310 6743-6749 Gene_expression denotes Levels
T4309 6743-6749 Gene_expression denotes Levels
T4308 6743-6749 Gene_expression denotes Levels
T4307 6743-6749 Gene_expression denotes Levels
T4306 6743-6749 Gene_expression denotes Levels
T4305 6837-6841 Protein denotes IL-6
T4304 6822-6835 Protein denotes interleukin-6
T4303 6810-6815 Protein denotes IL-1β
T4302 6790-6808 Protein denotes interleukin-1 beta
T4301 6782-6787 Protein denotes TNF-α
T4300 6753-6780 Protein denotes tumor necrosis factor-alpha
T3696 6475-6482 Protein denotes β-actin
T3695 6151-6173 Protein denotes horseradish peroxidase
T3694 5890-5894 Protein denotes MMP9
T4559 8258-8262 Protein denotes MMP9
T3231 4964-4971 Protein denotes β-actin
T3230 4935-4949 Protein denotes ImageJ program
T3229 4702-4724 Protein denotes GoTaq Green Master Mix
T2170 2466-2469 Protein denotes Rad
T2169 2330-2338 Protein denotes μL CCK-8
T1506 1331-1335 Protein denotes GFAP
T1505 261-265 Protein denotes Nrf2
T1504 86-90 Protein denotes Nrf2
R2312 T2672 T2673 themeOf NF-κB,binding
R3695 T4300 T4306 themeOf tumor necrosis factor-alpha,Levels
R3696 T4301 T4307 themeOf TNF-α,Levels
R3697 T4302 T4308 themeOf interleukin-1 beta,Levels
R3698 T4303 T4309 themeOf IL-1β,Levels
R3699 T4304 T4310 themeOf interleukin-6,Levels
R3700 T4305 T4311 themeOf IL-6,Levels

testone

Id Subject Object Predicate Lexical cue
T4373 8514-8518 Protein denotes MMP9
T4372 8258-8262 Protein denotes MMP9
T4043 6837-6841 Protein denotes IL-6
T4042 6822-6835 Protein denotes interleukin-6
T4041 6810-6815 Protein denotes IL-1β
T4040 6790-6808 Protein denotes interleukin-1 beta
T3097 4964-4971 Protein denotes β-actin
T3370 5890-5894 Protein denotes MMP9
T2032 2556-2565 Gene_expression denotes expressed
R3486 T4041 T4040 equivalentTo IL-1β,interleukin-1 beta
R3487 T4043 T4042 equivalentTo IL-6,interleukin-6

test3

Id Subject Object Predicate Lexical cue
T4377 8514-8518 Protein denotes MMP9
T4376 8258-8262 Protein denotes MMP9
T4375 8514-8518 Protein denotes MMP9
T4374 8258-8262 Protein denotes MMP9
T4051 6837-6841 Protein denotes IL-6
T4050 6822-6835 Protein denotes interleukin-6
T4049 6810-6815 Protein denotes IL-1β
T4048 6790-6808 Protein denotes interleukin-1 beta
T4047 6837-6841 Protein denotes IL-6
T4046 6822-6835 Protein denotes interleukin-6
T4045 6810-6815 Protein denotes IL-1β
T4044 6790-6808 Protein denotes interleukin-1 beta
T3099 4964-4971 Protein denotes β-actin
T3098 4964-4971 Protein denotes β-actin
T3372 5890-5894 Protein denotes MMP9
T3371 5890-5894 Protein denotes MMP9
R3488 T4049 T4048 equivalentTo IL-1β,interleukin-1 beta
R3489 T4051 T4050 equivalentTo IL-6,interleukin-6