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PMC:3317373 / 10541-11434 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T4496 575-577 TO denotes to
T4495 570-574 NN denotes MMP9
T4494 566-569 DT denotes the
T4493 559-565 VB denotes enable
T4492 556-558 TO denotes to
T4491 551-555 NN denotes 37°C
T4490 548-550 IN denotes at
T4489 546-547 NN denotes h
T4488 543-545 CD denotes 40
T4487 539-542 IN denotes for
T4486 532-538 NN denotes buffer
T4485 522-531 NN denotes substrate
T4484 519-521 IN denotes in
T4483 509-518 VB denotes incubated
T4482 504-508 RB denotes then
T4481 500-503 CC denotes and
T4480 494-499 NN denotes X-100
T4479 487-493 NN denotes Triton
T4478 485-486 NN denotes %
T4477 482-485 CD denotes 2.5
T4476 479-481 IN denotes in
T4475 468-478 NN denotes incubation
T4474 465-467 IN denotes by
T4473 455-464 VB denotes renatured
T4472 450-454 VB denotes were
T4471 441-449 NN denotes proteins
T4470 437-440 DT denotes the
T4469 432-436 RB denotes Then
T4468 415-430 NN denotes electrophoresis
T4467 412-414 IN denotes by
T4466 402-411 VB denotes separated
T4465 397-401 VB denotes were
T4464 388-396 NN denotes extracts
T4463 380-387 NN denotes protein
T4462 370-379 JJ denotes cytosolic
T4461 367-369 NN denotes μg
T4460 364-366 CD denotes 40
T4459 362-363 -COMMA- denotes ,
T4458 355-362 RB denotes Briefly
T4457 352-353 -RRB- denotes )
T4456 349-352 NNP denotes USA
T4455 347-348 -COMMA- denotes ,
T4454 345-347 NNP denotes MA
T4453 343-344 -COMMA- denotes ,
T4452 340-343 NNP denotes Inc
T4451 328-339 NNP denotes Scientifics
T4450 321-327 NNP denotes Genmed
T4449 320-321 -LRB- denotes (
T4448 307-319 NN denotes instructions
T4447 304-306 POS denotes 's
T4446 292-304 NN denotes manufacturer
T4445 288-291 DT denotes the
T4444 285-287 TO denotes to
T4443 275-284 VB denotes according
T4442 265-274 VB denotes performed
T4441 261-264 VB denotes was
T4440 250-260 NN denotes zymography
T4439 242-249 NN denotes Gelatin
T4438 235-240 NN denotes –20°C
T4437 232-234 IN denotes at
T4436 225-231 VB denotes stored
T4435 221-224 CC denotes and
T4434 206-220 NN denotes centrifugation
T4433 203-205 IN denotes by
T4432 193-202 VB denotes separated
T4431 188-192 VB denotes were
T4430 179-187 NN denotes extracts
T4429 171-178 NNP denotes Soluble
T4428 163-170 NNP denotes Triton.
T4427 161-162 NN denotes %
T4426 157-161 CD denotes 0.01
T4425 153-156 CC denotes and
T4424 151-152 -COMMA- denotes ,
T4423 147-151 NN denotes NaN3
T4422 144-146 NN denotes mM
T4421 140-143 CD denotes 0.2
T4420 138-139 -COMMA- denotes ,
T4419 133-138 NN denotes CaCl2
T4418 130-132 NN denotes mM
T4417 128-129 CD denotes 5
T4416 126-127 -COMMA- denotes ,
T4415 122-126 NN denotes NaCl
T4414 119-121 NN denotes mM
T4413 115-118 CD denotes 150
T4412 113-114 -COMMA- denotes ,
T4411 112-113 -RRB- denotes )
T4410 109-112 CD denotes 7.4
T4409 106-108 NN denotes pH
T4408 105-106 -LRB- denotes (
T4407 96-104 NN denotes Tris-HCl
T4406 93-95 NN denotes mM
T4405 90-92 CD denotes 50
T4404 79-89 VB denotes containing
T4403 72-78 NN denotes buffer
T4402 66-71 NN denotes lysis
T4401 63-65 IN denotes in
T4400 51-62 VB denotes homogenized
T4399 46-50 VB denotes were
T4398 39-45 NN denotes groups
T4397 34-38 CD denotes four
T4396 31-33 IN denotes of
T4395 25-30 NN denotes Cells
T4394 14-24 NN denotes Zymography
T4393 5-13 NN denotes Gelatine
T4392 0-3 CD denotes 2.8
T4549 886-892 NN denotes ImageJ
T4548 878-885 NN denotes program
T4547 869-877 NN denotes analysis
T4546 863-868 NN denotes image
T4545 858-862 IN denotes with
T4544 848-857 VB denotes performed
T4543 844-847 VB denotes was
T4542 836-843 NN denotes density
T4541 831-835 NN denotes band
T4540 826-830 NN denotes MMP9
T4539 823-825 IN denotes of
T4538 808-822 NN denotes Quantification
T4537 797-806 NN denotes substrate
T4536 793-796 DT denotes the
T4535 790-792 IN denotes of
T4534 784-789 NN denotes lysis
T4533 780-783 DT denotes the
T4532 770-779 VB denotes indicates
T4531 764-769 WDT denotes which
T4530 760-763 NN denotes gel
T4529 755-759 JJ denotes blue
T4528 752-754 IN denotes in
T4527 746-751 NN denotes bands
T4526 740-745 JJ denotes clear
T4525 737-739 IN denotes by
T4524 730-736 VB denotes showed
T4523 725-729 VB denotes were
T4522 714-724 NN denotes activities
T4521 702-713 JJ denotes Proteolytic
T4520 692-700 NN denotes methanol
T4519 690-691 NN denotes %
T4518 688-690 CD denotes 50
T4517 685-687 IN denotes in
T4516 675-684 VB denotes destained
T4515 671-674 CC denotes and
T4514 669-670 NN denotes h
T4513 667-668 CD denotes 1
T4512 663-666 IN denotes for
T4511 658-662 JJ denotes blue
T4510 648-657 NNP denotes Coomassie
T4509 643-647 IN denotes with
T4508 635-642 VB denotes stained
T4507 631-634 VB denotes was
T4506 627-630 NN denotes gel
T4505 622-626 DT denotes each
T4504 620-621 -COMMA- denotes ,
T4503 615-620 NN denotes water
T4502 612-614 IN denotes in
T4501 604-611 VB denotes rinsing
T4500 598-603 IN denotes After
T4499 589-596 NN denotes gelatin
T4498 585-588 DT denotes the
T4497 578-584 VB denotes cleave
R3709 T4394 T4393 arg1Of Zymography,Gelatine
R3710 T4395 T4396 arg1Of Cells,of
R3711 T4395 T4399 arg1Of Cells,were
R3712 T4395 T4400 arg2Of Cells,homogenized
R3713 T4398 T4396 arg2Of groups,of
R3714 T4398 T4397 arg1Of groups,four
R3715 T4400 T4399 arg2Of homogenized,were
R3716 T4400 T4401 arg1Of homogenized,in
R3717 T4400 T4425 arg1Of homogenized,and
R3718 T4403 T4401 arg2Of buffer,in
R3719 T4403 T4402 arg1Of buffer,lysis
R3720 T4403 T4404 arg1Of buffer,containing
R3721 T4405 T4406 arg1Of 50,mM
R3722 T4407 T4405 arg1Of Tris-HCl,50
R3723 T4407 T4408 arg1Of Tris-HCl,(
R3724 T4407 T4412 arg1Of Tris-HCl,","
R3725 T4409 T4408 arg2Of pH,(
R3726 T4409 T4410 arg1Of pH,7.4
R3727 T4411 T4408 arg3Of ),(
R3728 T4412 T4416 arg1Of ",",","
R3729 T4413 T4414 arg1Of 150,mM
R3730 T4415 T4412 arg2Of NaCl,","
R3731 T4415 T4413 arg1Of NaCl,150
R3732 T4416 T4420 arg1Of ",",","
R3733 T4417 T4418 arg1Of 5,mM
R3734 T4419 T4416 arg2Of CaCl2,","
R3735 T4419 T4417 arg1Of CaCl2,5
R3736 T4420 T4404 arg2Of ",",containing
R3737 T4421 T4422 arg1Of 0.2,mM
R3738 T4423 T4420 arg2Of NaN3,","
R3739 T4423 T4421 arg1Of NaN3,0.2
R3740 T4425 T4424 arg1Of and,","
R3741 T4427 T4426 arg1Of %,0.01
R3742 T4429 T4428 arg1Of Soluble,Triton.
R3743 T4430 T4429 arg1Of extracts,Soluble
R3744 T4430 T4431 arg1Of extracts,were
R3745 T4430 T4432 arg2Of extracts,separated
R3746 T4430 T4436 arg2Of extracts,stored
R3747 T4432 T4435 arg1Of separated,and
R3748 T4434 T4432 arg1Of centrifugation,separated
R3749 T4434 T4433 arg2Of centrifugation,by
R3750 T4435 T4425 arg2Of and,and
R3751 T4435 T4427 arg1Of and,%
R3752 T4435 T4431 arg2Of and,were
R3753 T4436 T4435 arg2Of stored,and
R3754 T4436 T4437 arg1Of stored,at
R3755 T4438 T4437 arg2Of –20°C,at
R3756 T4440 T4439 arg1Of zymography,Gelatin
R3757 T4440 T4441 arg1Of zymography,was
R3758 T4440 T4442 arg2Of zymography,performed
R3759 T4442 T4441 arg2Of performed,was
R3760 T4442 T4443 arg1Of performed,according
R3761 T4444 T4443 arg2Of to,according
R3762 T4446 T4445 arg1Of manufacturer,the
R3763 T4446 T4447 arg2Of manufacturer,'s
R3764 T4448 T4444 arg2Of instructions,to
R3765 T4448 T4447 arg1Of instructions,'s
R3766 T4448 T4449 arg1Of instructions,(
R3767 T4452 T4449 arg2Of Inc,(
R3768 T4452 T4450 arg1Of Inc,Genmed
R3769 T4452 T4451 arg1Of Inc,Scientifics
R3770 T4452 T4453 arg1Of Inc,","
R3771 T4454 T4453 arg2Of MA,","
R3772 T4454 T4455 arg1Of MA,","
R3773 T4456 T4455 arg2Of USA,","
R3774 T4457 T4449 arg3Of ),(
R3775 T4464 T4460 arg1Of extracts,40
R3776 T4464 T4461 arg1Of extracts,μg
R3777 T4464 T4462 arg1Of extracts,cytosolic
R3778 T4464 T4463 arg1Of extracts,protein
R3779 T4464 T4465 arg1Of extracts,were
R3780 T4464 T4466 arg2Of extracts,separated
R3781 T4466 T4458 arg1Of separated,Briefly
R3782 T4466 T4459 arg1Of separated,","
R3783 T4466 T4465 arg2Of separated,were
R3784 T4468 T4466 arg1Of electrophoresis,separated
R3785 T4468 T4467 arg2Of electrophoresis,by
R3786 T4471 T4470 arg1Of proteins,the
R3787 T4471 T4472 arg1Of proteins,were
R3788 T4471 T4473 arg2Of proteins,renatured
R3789 T4471 T4483 arg2Of proteins,incubated
R3790 T4473 T4474 arg1Of renatured,by
R3791 T4473 T4481 arg1Of renatured,and
R3792 T4475 T4474 arg2Of incubation,by
R3793 T4475 T4476 arg1Of incubation,in
R3794 T4477 T4478 arg1Of 2.5,%
R3795 T4480 T4476 arg2Of X-100,in
R3796 T4480 T4477 arg1Of X-100,2.5
R3797 T4480 T4479 arg1Of X-100,Triton
R3798 T4481 T4469 arg1Of and,Then
R3799 T4481 T4472 arg2Of and,were
R3800 T4483 T4481 arg2Of incubated,and
R3801 T4483 T4482 arg1Of incubated,then
R3802 T4483 T4484 arg1Of incubated,in
R3803 T4483 T4487 arg1Of incubated,for
R3804 T4483 T4490 arg1Of incubated,at
R3805 T4483 T4492 modOf incubated,to
R3806 T4486 T4484 arg2Of buffer,in
R3807 T4486 T4485 arg1Of buffer,substrate
R3808 T4489 T4487 arg2Of h,for
R3809 T4489 T4488 arg1Of h,40
R3810 T4491 T4490 arg2Of 37°C,at
R3811 T4493 T4492 arg1Of enable,to
R3812 T4495 T4493 arg2Of MMP9,enable
R3813 T4495 T4494 arg1Of MMP9,the
R3814 T4495 T4497 arg1Of MMP9,cleave
R3815 T4497 T4493 arg3Of cleave,enable
R3816 T4497 T4496 arg1Of cleave,to
R3817 T4499 T4497 arg2Of gelatin,cleave
R3818 T4499 T4498 arg1Of gelatin,the
R3819 T4501 T4500 arg2Of rinsing,After
R3820 T4501 T4502 arg1Of rinsing,in
R3821 T4503 T4502 arg2Of water,in
R3822 T4506 T4501 arg1Of gel,rinsing
R3823 T4506 T4505 arg1Of gel,each
R3824 T4506 T4507 arg1Of gel,was
R3825 T4506 T4508 arg2Of gel,stained
R3826 T4506 T4511 arg1Of gel,blue
R3827 T4506 T4516 arg2Of gel,destained
R3828 T4508 T4509 arg1Of stained,with
R3829 T4508 T4515 arg1Of stained,and
R3830 T4510 T4509 arg2Of Coomassie,with
R3831 T4511 T4508 arg3Of blue,stained
R3832 T4511 T4512 arg1Of blue,for
R3833 T4514 T4512 arg2Of h,for
R3834 T4514 T4513 arg1Of h,1
R3835 T4515 T4500 arg1Of and,After
R3836 T4515 T4504 arg1Of and,","
R3837 T4515 T4507 arg2Of and,was
R3838 T4516 T4515 arg2Of destained,and
R3839 T4516 T4517 arg1Of destained,in
R3840 T4518 T4519 arg1Of 50,%
R3841 T4520 T4517 arg2Of methanol,in
R3842 T4520 T4518 arg1Of methanol,50
R3843 T4522 T4521 arg1Of activities,Proteolytic
R3844 T4522 T4523 arg1Of activities,were
R3845 T4522 T4524 arg2Of activities,showed
R3846 T4524 T4523 arg2Of showed,were
R3847 T4527 T4524 arg1Of bands,showed
R3848 T4527 T4525 arg2Of bands,by
R3849 T4527 T4526 arg1Of bands,clear
R3850 T4527 T4528 arg1Of bands,in
R3851 T4530 T4528 arg2Of gel,in
R3852 T4530 T4529 arg1Of gel,blue
R3853 T4530 T4531 arg1Of gel,which
R3854 T4530 T4532 arg1Of gel,indicates
R3855 T4534 T4532 arg2Of lysis,indicates
R3856 T4534 T4533 arg1Of lysis,the
R3857 T4534 T4535 arg1Of lysis,of
R3858 T4537 T4535 arg2Of substrate,of
R3859 T4537 T4536 arg1Of substrate,the
R3860 T4538 T4539 arg1Of Quantification,of
R3861 T4538 T4543 arg1Of Quantification,was
R3862 T4538 T4544 arg2Of Quantification,performed
R3863 T4542 T4539 arg2Of density,of
R3864 T4542 T4540 arg1Of density,MMP9
R3865 T4542 T4541 arg1Of density,band
R3866 T4544 T4543 arg2Of performed,was
R3867 T4544 T4545 arg1Of performed,with
R3868 T4549 T4545 arg2Of ImageJ,with
R3869 T4549 T4546 arg1Of ImageJ,image
R3870 T4549 T4547 arg1Of ImageJ,analysis
R3871 T4549 T4548 arg1Of ImageJ,program

bionlp-st-ge-2016-test-proteins

Id Subject Object Predicate Lexical cue
T4389 826-830 Protein denotes MMP9
T4388 570-574 Protein denotes MMP9

GO-BP

Id Subject Object Predicate Lexical cue
T4391 784-789 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T4390 66-71 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis

sentences

Id Subject Object Predicate Lexical cue
T4385 808-893 Sentence denotes Quantification of MMP9 band density was performed with image analysis program ImageJ.
T4384 702-807 Sentence denotes Proteolytic activities were showed by clear bands in blue gel which indicates the lysis of the substrate.
T4383 598-701 Sentence denotes After rinsing in water, each gel was stained with Coomassie blue for 1 h and destained in 50% methanol.
T4382 432-597 Sentence denotes Then the proteins were renatured by incubation in 2.5% Triton X-100 and then incubated in substrate buffer for 40 h at 37°C to enable the MMP9 to cleave the gelatin.
T4381 355-431 Sentence denotes Briefly, 40 μg cytosolic protein extracts were separated by electrophoresis.
T4380 25-354 Sentence denotes Cells of four groups were homogenized in lysis buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 5 mM CaCl2, 0.2 mM NaN3, and 0.01% Triton. Soluble extracts were separated by centrifugation and stored at –20°C. Gelatin zymography was performed according to the manufacturer's instructions (Genmed Scientifics Inc, MA, USA).
T4379 5-24 Sentence denotes Gelatine Zymography
T4378 0-4 Sentence denotes 2.8.
T107 0-4 Sentence denotes 2.8.
T108 5-24 Sentence denotes Gelatine Zymography
T109 25-170 Sentence denotes Cells of four groups were homogenized in lysis buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 5 mM CaCl2, 0.2 mM NaN3, and 0.01% Triton.
T110 171-241 Sentence denotes Soluble extracts were separated by centrifugation and stored at –20°C.
T111 242-354 Sentence denotes Gelatin zymography was performed according to the manufacturer's instructions (Genmed Scientifics Inc, MA, USA).
T112 355-431 Sentence denotes Briefly, 40 μg cytosolic protein extracts were separated by electrophoresis.
T113 432-597 Sentence denotes Then the proteins were renatured by incubation in 2.5% Triton X-100 and then incubated in substrate buffer for 40 h at 37°C to enable the MMP9 to cleave the gelatin.
T114 598-701 Sentence denotes After rinsing in water, each gel was stained with Coomassie blue for 1 h and destained in 50% methanol.
T115 702-807 Sentence denotes Proteolytic activities were showed by clear bands in blue gel which indicates the lysis of the substrate.
T116 808-893 Sentence denotes Quantification of MMP9 band density was performed with image analysis program ImageJ.

simple1

Id Subject Object Predicate Lexical cue
T4551 826-830 Protein denotes MMP9
T4550 570-574 Protein denotes MMP9

BioNLP16_DUT

Id Subject Object Predicate Lexical cue
T4732 826-830 Protein denotes MMP9
T4731 570-574 Protein denotes MMP9

BioNLP16_Messiy

Id Subject Object Predicate Lexical cue
T4556 578-584 Protein_catabolism denotes cleave
T4555 826-830 Protein denotes MMP9
T4554 570-574 Protein denotes MMP9
R3872 T4554 T4556 themeOf MMP9,cleave

DLUT931

Id Subject Object Predicate Lexical cue
T4553 826-830 Protein denotes MMP9
T4552 570-574 Protein denotes MMP9

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T4752 5-13 Entity denotes Gelatine
T4751 522-531 Entity denotes substrate
T4750 364-396 Entity denotes 40 μg cytosolic protein extracts
T4749 25-45 Entity denotes Cells of four groups
T4748 667-670 Entity denotes 1 h
T4747 482-499 Entity denotes 2.5% Triton X-100
T4746 171-187 Entity denotes Soluble extracts
T4745 566-574 Protein denotes the MMP9
T4744 140-169 Entity denotes 0.2 mM NaN3, and 0.01% Triton
T4743 157-169 Entity denotes 0.01% Triton
T4742 90-104 Entity denotes 50 mM Tris-HCl
T4741 755-806 Entity denotes blue gel which indicates the lysis of the substrate
T4740 115-126 Entity denotes 150 mM NaCl
T4739 622-630 Entity denotes each gel
T4738 826-830 Protein denotes MMP9
T4737 364-396 Protein denotes 40 μg cytosolic protein extracts
T4736 242-249 Entity denotes Gelatin
T4735 437-449 Protein denotes the proteins
T4734 90-104 Entity denotes 50 mM Tris-HCl
T4733 688-700 Entity denotes 50% methanol

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T4729 892-893 . denotes .
T4728 886-892 NNP denotes ImageJ
T4727 878-885 NN denotes program
T4726 869-877 NN denotes analysis
T4725 863-868 NN denotes image
T4723 848-857 VBN denotes performed
T4722 844-847 VBD denotes was
T4721 836-843 NN denotes density
T4720 831-835 NN denotes band
T4719 826-830 NNP denotes MMP9
T4718 823-825 IN denotes of
T4717 808-822 NN denotes Quantification
T4716 806-807 . denotes .
T4715 797-806 NN denotes substrate
T4714 793-796 DT denotes the
T4713 790-792 IN denotes of
T4712 784-789 NN denotes lysis
T4711 780-783 DT denotes the
T4710 770-779 VBZ denotes indicates
T4709 764-769 WDT denotes which
T4708 760-763 NN denotes gel
T4707 755-759 JJ denotes blue
T4706 752-754 IN denotes in
T4705 746-751 NNS denotes bands
T4704 740-745 JJ denotes clear
T4703 737-739 IN denotes by
T4702 730-736 VBN denotes showed
T4701 725-729 VBD denotes were
T4700 714-724 NNS denotes activities
T4699 702-713 JJ denotes Proteolytic
T4698 700-701 . denotes .
T4697 692-700 NN denotes methanol
T4696 690-691 NN denotes %
T4695 688-690 CD denotes 50
T4694 685-687 IN denotes in
T4693 675-684 VBN denotes destained
T4692 671-674 CC denotes and
T4686 643-647 IN denotes with
T4685 635-642 VBN denotes stained
T4684 631-634 VBD denotes was
T4683 627-630 NN denotes gel
T4682 622-626 DT denotes each
T4652 485-486 NN denotes %
T4651 482-485 CD denotes 2.5
T4650 479-481 IN denotes in
T4649 468-478 NN denotes incubation
T4648 465-467 IN denotes by
T4647 455-464 VBN denotes renatured
T4646 450-454 VBD denotes were
T4645 441-449 NNS denotes proteins
T4644 437-440 DT denotes the
T4643 432-436 RB denotes Then
T4642 430-431 . denotes .
T4641 415-430 NN denotes electrophoresis
T4640 412-414 IN denotes by
T4639 402-411 VBN denotes separated
T4638 397-401 VBD denotes were
T4637 388-396 NNS denotes extracts
T4636 380-387 NN denotes protein
T4635 370-379 JJ denotes cytosolic
T4634 367-369 NN denotes μg
T4633 364-366 CD denotes 40
T4632 362-363 , denotes ,
T4631 355-362 RB denotes Briefly
T4630 353-354 . denotes .
T4629 352-353 -RRB- denotes )
T4628 349-352 NNP denotes USA
T4627 347-348 , denotes ,
T4626 345-347 NNP denotes MA
T4625 343-344 , denotes ,
T4624 340-343 NNP denotes Inc
T4623 328-339 NNP denotes Scientifics
T4622 321-327 NNP denotes Genmed
T4621 320-321 -LRB- denotes (
T4620 307-319 NNS denotes instructions
T4619 304-306 POS denotes 's
T4618 292-304 NN denotes manufacturer
T4617 288-291 DT denotes the
T4616 285-287 TO denotes to
T4615 275-284 VBG denotes according
T4614 265-274 VBN denotes performed
T4613 261-264 VBD denotes was
T4612 250-260 NN denotes zymography
T4611 242-249 NNP denotes Gelatin
T4610 239-241 NNP denotes C.
T4609 238-239 NN denotes °
T4608 236-238 CD denotes 20
T4607 232-234 IN denotes at
T4606 225-231 VBD denotes stored
T4605 221-224 CC denotes and
T4604 206-220 NN denotes centrifugation
T4603 203-205 IN denotes by
T4602 193-202 VBN denotes separated
T4601 188-192 VBD denotes were
T4600 179-187 NNS denotes extracts
T4599 171-178 JJ denotes Soluble
T4598 169-170 . denotes .
T4597 163-169 NNP denotes Triton
T4596 161-162 NN denotes %
T4595 157-161 CD denotes 0.01
T4594 153-156 CC denotes and
T4593 151-152 , denotes ,
T4592 147-151 NNP denotes NaN3
T4591 144-146 NNP denotes mM
T4590 140-143 CD denotes 0.2
T4589 138-139 , denotes ,
T4588 133-138 NNP denotes CaCl2
T4587 130-132 NNP denotes mM
T4586 128-129 CD denotes 5
T4585 126-127 , denotes ,
T4584 122-126 NNP denotes NaCl
T4583 119-121 NNP denotes mM
T4582 115-118 CD denotes 150
T4581 113-114 , denotes ,
T4580 112-113 -RRB- denotes )
T4579 109-112 CD denotes 7.4
T4578 106-108 NNP denotes pH
T4577 105-106 -LRB- denotes (
T4576 96-104 NNP denotes Tris-HCl
T4575 93-95 NNP denotes mM
T4574 90-92 CD denotes 50
T4573 79-89 VBG denotes containing
T4572 72-78 NN denotes buffer
T4571 66-71 NN denotes lysis
T4570 63-65 IN denotes in
T4569 51-62 VBN denotes homogenized
T4568 46-50 VBD denotes were
T4567 39-45 NNS denotes groups
T4566 34-38 CD denotes four
T4565 31-33 IN denotes of
T4564 25-30 NNP denotes Cells
T4563 14-24 NNP denotes Zymography
T4562 5-13 NNP denotes Gelatine
T4561 3-4 . denotes .
T4560 0-3 CD denotes 2.8
T4691 669-670 NN denotes h
T4690 667-668 CD denotes 1
T4689 663-666 IN denotes for
T4688 658-662 JJ denotes blue
T4687 648-657 NNP denotes Coomassie
T4681 620-621 , denotes ,
T4680 615-620 NN denotes water
T4679 612-614 IN denotes in
T4678 604-611 VBG denotes rinsing
T4677 598-603 IN denotes After
T4676 596-597 . denotes .
T4675 589-596 NN denotes gelatin
T4674 585-588 DT denotes the
T4673 578-584 VB denotes cleave
T4672 575-577 TO denotes to
T4671 570-574 NNP denotes MMP9
T4670 566-569 DT denotes the
T4669 559-565 VB denotes enable
T4668 556-558 TO denotes to
T4667 554-555 NNP denotes C
T4666 553-554 CD denotes °
T4665 551-553 CD denotes 37
T4664 548-550 IN denotes at
T4663 546-547 NN denotes h
T4662 543-545 CD denotes 40
T4661 539-542 IN denotes for
T4660 532-538 NN denotes buffer
T4659 522-531 JJ denotes substrate
T4658 519-521 IN denotes in
T4657 509-518 VBD denotes incubated
T4656 504-508 RB denotes then
T4655 500-503 CC denotes and
T4654 494-499 NNP denotes X-100
T4653 487-493 NNP denotes Triton
T4724 858-862 IN denotes with
R3873 T4560 T4560 ROOT 2.8,2.8
R3874 T4561 T4560 punct .,2.8
R3875 T4562 T4564 compound Gelatine,Cells
R3887 T4574 T4576 nummod 50,Tris-HCl
R3888 T4575 T4576 compound mM,Tris-HCl
R3889 T4576 T4573 dobj Tris-HCl,containing
R3890 T4577 T4578 punct (,pH
R3891 T4578 T4576 appos pH,Tris-HCl
R3892 T4579 T4578 nummod 7.4,pH
R3893 T4580 T4578 punct ),pH
R3894 T4581 T4576 punct ",",Tris-HCl
R3895 T4582 T4584 nummod 150,NaCl
R3896 T4583 T4584 compound mM,NaCl
R3897 T4584 T4576 conj NaCl,Tris-HCl
R3898 T4585 T4584 punct ",",NaCl
R3899 T4586 T4588 nummod 5,CaCl2
R3900 T4587 T4588 compound mM,CaCl2
R3901 T4588 T4584 conj CaCl2,NaCl
R3902 T4589 T4588 punct ",",CaCl2
R3903 T4590 T4592 nummod 0.2,NaN3
R3904 T4591 T4592 compound mM,NaN3
R3905 T4592 T4588 conj NaN3,CaCl2
R3906 T4593 T4592 punct ",",NaN3
R3907 T4594 T4592 cc and,NaN3
R3908 T4595 T4596 nummod 0.01,%
R3909 T4596 T4597 compound %,Triton
R3910 T4597 T4592 conj Triton,NaN3
R3911 T4598 T4569 punct .,homogenized
R3912 T4599 T4600 amod Soluble,extracts
R3913 T4600 T4602 nsubjpass extracts,separated
R3914 T4601 T4602 auxpass were,separated
R3915 T4602 T4602 ROOT separated,separated
R3916 T4603 T4602 agent by,separated
R3917 T4604 T4603 pobj centrifugation,by
R3918 T4605 T4602 cc and,separated
R3919 T4606 T4614 nsubjpass stored,performed
R3920 T4607 T4606 prep at,stored
R3921 T4608 T4609 nummod 20,°
R3922 T4609 T4612 compound °,zymography
R3923 T4610 T4612 compound C.,zymography
R3924 T4611 T4612 compound Gelatin,zymography
R3925 T4612 T4607 pobj zymography,at
R3926 T4613 T4614 auxpass was,performed
R3927 T4614 T4602 conj performed,separated
R3928 T4615 T4614 xcomp according,performed
R3929 T4616 T4615 prep to,according
R3930 T4617 T4618 det the,manufacturer
R3931 T4618 T4620 poss manufacturer,instructions
R3932 T4619 T4618 case 's,manufacturer
R3933 T4620 T4616 pobj instructions,to
R3934 T4621 T4624 punct (,Inc
R3935 T4622 T4624 compound Genmed,Inc
R3936 T4623 T4624 compound Scientifics,Inc
R3937 T4624 T4620 appos Inc,instructions
R3938 T4625 T4624 punct ",",Inc
R3939 T4626 T4624 conj MA,Inc
R3940 T4627 T4626 punct ",",MA
R3941 T4628 T4626 appos USA,MA
R3942 T4629 T4626 punct ),MA
R3943 T4630 T4614 punct .,performed
R3944 T4631 T4639 advmod Briefly,separated
R3945 T4632 T4639 punct ",",separated
R3946 T4633 T4637 nummod 40,extracts
R3947 T4634 T4637 compound μg,extracts
R3948 T4635 T4636 compound cytosolic,protein
R3949 T4636 T4637 compound protein,extracts
R3950 T4637 T4639 nsubjpass extracts,separated
R3951 T4638 T4639 auxpass were,separated
R3952 T4639 T4639 ROOT separated,separated
R3953 T4640 T4639 prep by,separated
R3954 T4641 T4640 pobj electrophoresis,by
R3955 T4642 T4639 punct .,separated
R3956 T4643 T4647 advmod Then,renatured
R3957 T4644 T4645 det the,proteins
R3958 T4645 T4647 nsubjpass proteins,renatured
R3959 T4646 T4647 auxpass were,renatured
R3960 T4647 T4647 ROOT renatured,renatured
R3961 T4648 T4647 agent by,renatured
R3962 T4649 T4648 pobj incubation,by
R3963 T4650 T4647 prep in,renatured
R3975 T4662 T4663 nummod 40,h
R3976 T4663 T4661 pobj h,for
R3977 T4664 T4657 prep at,incubated
R3978 T4665 T4666 nummod 37,°
R3979 T4666 T4664 pobj °,at
R3980 T4667 T4657 conj C,incubated
R3981 T4668 T4669 aux to,enable
R3982 T4669 T4657 advcl enable,incubated
R3983 T4670 T4671 det the,MMP9
R3984 T4671 T4669 dobj MMP9,enable
R3985 T4672 T4673 aux to,cleave
R3986 T4673 T4669 xcomp cleave,enable
R3987 T4674 T4675 det the,gelatin
R3988 T4675 T4673 dobj gelatin,cleave
R3989 T4676 T4647 punct .,renatured
R3990 T4677 T4685 prep After,stained
R3991 T4678 T4677 pcomp rinsing,After
R3992 T4679 T4678 prep in,rinsing
R3993 T4680 T4679 pobj water,in
R3994 T4681 T4685 punct ",",stained
R3995 T4682 T4683 det each,gel
R3996 T4683 T4685 nsubjpass gel,stained
R3997 T4684 T4685 auxpass was,stained
R3998 T4685 T4685 ROOT stained,stained
R3999 T4686 T4685 prep with,stained
R4000 T4687 T4688 compound Coomassie,blue
R4001 T4688 T4686 pobj blue,with
R4002 T4689 T4688 prep for,blue
R4003 T4690 T4691 nummod 1,h
R4004 T4691 T4689 pobj h,for
R4005 T4692 T4691 cc and,h
R4006 T4693 T4688 conj destained,blue
R4007 T4694 T4693 prep in,destained
R4008 T4695 T4696 nummod 50,%
R4009 T4696 T4697 compound %,methanol
R4010 T4697 T4694 pobj methanol,in
R4011 T4698 T4685 punct .,stained
R4012 T4699 T4700 amod Proteolytic,activities
R4013 T4700 T4702 nsubjpass activities,showed
R4014 T4701 T4702 auxpass were,showed
R4015 T4702 T4702 ROOT showed,showed
R4016 T4703 T4702 agent by,showed
R4017 T4704 T4705 amod clear,bands
R4018 T4705 T4703 pobj bands,by
R4019 T4706 T4705 prep in,bands
R4020 T4707 T4708 amod blue,gel
R4021 T4708 T4706 pobj gel,in
R4022 T4709 T4710 nsubj which,indicates
R4023 T4710 T4702 advcl indicates,showed
R4024 T4711 T4712 det the,lysis
R4025 T4712 T4710 dobj lysis,indicates
R4026 T4713 T4712 prep of,lysis
R4027 T4714 T4715 det the,substrate
R4028 T4715 T4713 pobj substrate,of
R4029 T4716 T4702 punct .,showed
R4030 T4717 T4723 nsubjpass Quantification,performed
R4031 T4718 T4717 prep of,Quantification
R3876 T4563 T4564 compound Zymography,Cells
R3877 T4564 T4569 nsubjpass Cells,homogenized
R3878 T4565 T4564 prep of,Cells
R3879 T4566 T4567 nummod four,groups
R3880 T4567 T4565 pobj groups,of
R3881 T4568 T4569 auxpass were,homogenized
R3882 T4569 T4569 ROOT homogenized,homogenized
R3883 T4570 T4569 prep in,homogenized
R3884 T4571 T4572 compound lysis,buffer
R3885 T4572 T4570 pobj buffer,in
R3886 T4573 T4572 acl containing,buffer
R3964 T4651 T4652 nummod 2.5,%
R3965 T4652 T4654 compound %,X-100
R3966 T4653 T4654 compound Triton,X-100
R3967 T4654 T4650 pobj X-100,in
R3968 T4655 T4647 cc and,renatured
R3969 T4656 T4657 advmod then,incubated
R3970 T4657 T4647 conj incubated,renatured
R3971 T4658 T4657 prep in,incubated
R3972 T4659 T4660 compound substrate,buffer
R3973 T4660 T4658 pobj buffer,in
R3974 T4661 T4660 prep for,buffer
R4032 T4719 T4721 compound MMP9,density
R4033 T4720 T4721 compound band,density
R4034 T4721 T4718 pobj density,of
R4035 T4722 T4723 auxpass was,performed
R4036 T4723 T4723 ROOT performed,performed
R4037 T4724 T4723 prep with,performed
R4038 T4725 T4726 compound image,analysis
R4039 T4726 T4727 compound analysis,program
R4040 T4727 T4728 compound program,ImageJ
R4041 T4728 T4724 pobj ImageJ,with
R4042 T4729 T4723 punct .,performed

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T4559 570-574 Protein denotes MMP9

testone

Id Subject Object Predicate Lexical cue
T4373 826-830 Protein denotes MMP9
T4372 570-574 Protein denotes MMP9

test3

Id Subject Object Predicate Lexical cue
T4377 826-830 Protein denotes MMP9
T4376 570-574 Protein denotes MMP9
T4375 826-830 Protein denotes MMP9
T4374 570-574 Protein denotes MMP9