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2_test

Id Subject Object Predicate Lexical cue
22529521-10698075-67992081 1782-1783 10698075 denotes 1
22529521-16230799-67992082 1888-1889 16230799 denotes 2
22529521-15680377-67992082 1888-1889 15680377 denotes 2
22529521-18715849-67992082 1888-1889 18715849 denotes 2
22529521-19125683-67992083 2247-2248 19125683 denotes 5
22529521-20953205-67992084 2385-2386 20953205 denotes 6
22529521-8455207-67992085 4430-4431 8455207 denotes 7
22529521-16230799-67992086 12394-12395 16230799 denotes 2
22529521-16230799-67992087 13046-13047 16230799 denotes 2
22529521-17993453-67992088 13049-13050 17993453 denotes 8
22529521-19125683-67992089 13201-13202 19125683 denotes 5
22529521-16230799-67992090 15924-15925 16230799 denotes 2
22529521-9547234-67992091 15927-15928 9547234 denotes 9
22529521-15680377-67992092 16138-16139 15680377 denotes 3
22529521-19574840-67992093 16141-16143 19574840 denotes 10
22529521-19577550-67992094 16145-16147 19577550 denotes 11
22529521-8945720-67992095 16516-16518 8945720 denotes 12
22529521-8719627-67992096 16520-16522 8719627 denotes 13
22529521-21114487-67992097 16597-16599 21114487 denotes 14
22529521-15269258-67992098 16659-16661 15269258 denotes 15
22529521-10340762-67992099 16663-16665 10340762 denotes 16
22529521-19430250-67992100 17152-17154 19430250 denotes 17
22529521-18162315-67992101 17369-17371 18162315 denotes 18
22529521-19515491-67992102 17631-17633 19515491 denotes 19
22529521-15856023-67992103 18448-18450 15856023 denotes 21
22529521-15139523-67992104 18590-18592 15139523 denotes 22
22529521-18715849-67992105 18767-18768 18715849 denotes 4
22529521-19125683-67992106 18770-18771 19125683 denotes 5
22529521-19394962-67992107 18773-18775 19394962 denotes 23
22529521-16585964-67992108 19043-19045 16585964 denotes 24
22529521-18241676-67992109 19298-19300 18241676 denotes 25
22529521-20486765-67992110 19479-19481 20486765 denotes 26
22529521-18162315-67992111 19571-19573 18162315 denotes 18
22529521-7572279-67992112 19927-19929 7572279 denotes 27
22529521-21833844-67992113 19931-19933 21833844 denotes 28
22529521-19507272-67992114 20085-20087 19507272 denotes 29

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T53 0-3 DT denotes The
T54 4-11 NN denotes Absence
T55 12-14 IN denotes of
T56 15-19 NN denotes Nrf2
T57 20-28 VB denotes Enhances
T58 29-44 JJ denotes NF-κB-Dependent
T59 45-57 NN denotes Inflammation
T60 58-67 VB denotes following
T61 68-75 NNP denotes Scratch
T62 76-82 NNP denotes Injury
T63 83-85 IN denotes in
T64 86-91 NNP denotes Mouse
T65 92-99 NNP denotes Primary
T66 100-108 NNP denotes Cultured
T67 109-119 NN denotes Astrocytes
T1878 4635-4642 NN denotes scratch
T8267 20735-20738 NN denotes TBI
T8266 20729-20734 IN denotes after
T8265 20721-20728 NN denotes therapy
T8264 20703-20720 JJ denotes anti-inflammatory
T8263 20699-20702 IN denotes for
T8262 20692-20698 NN denotes target
T8261 20682-20691 JJ denotes important
T8260 20679-20681 DT denotes an
T8259 20676-20678 VB denotes be
T8258 20672-20675 MD denotes may
T8257 20667-20671 NN denotes Nrf2
T8256 20662-20666 IN denotes that
T8255 20654-20661 VB denotes suggest
T8254 20646-20653 NN denotes results
T8253 20640-20645 DT denotes These
T8252 20632-20638 NN denotes injury
T8251 20624-20631 NN denotes scratch
T8250 20618-20623 IN denotes after
T8249 20607-20617 NN denotes astrocytes
T8248 20604-20606 IN denotes in
T8247 20597-20603 NN denotes deaths
T8246 20592-20596 NN denotes cell
T8245 20587-20591 JJ denotes more
T8244 20584-20586 IN denotes in
T8243 20574-20583 VB denotes resulting
T8242 20569-20573 NN denotes MMP9
T8241 20565-20568 CC denotes and
T8240 20563-20564 -COMMA- denotes ,
T8239 20559-20563 NN denotes IL-6
T8238 20557-20558 -COMMA- denotes ,
T8237 20552-20557 NN denotes IL-1β
T8236 20550-20551 -COMMA- denotes ,
T8235 20545-20550 NN denotes TNF-α
T8234 20542-20544 IN denotes of
T8233 20531-20541 NN denotes expression
T8232 20527-20530 DT denotes the
T8231 20523-20526 CC denotes and
T8230 20517-20522 NN denotes NF-κB
T8229 20514-20516 IN denotes of
T8228 20503-20513 NN denotes activation
T8227 20499-20502 DT denotes the
T8226 20491-20498 VB denotes induced
T8225 20486-20490 NN denotes Nrf2
T8224 20483-20485 IN denotes of
T8223 20473-20482 NN denotes depletion
T8222 20471-20472 -COMMA- denotes ,
T8221 20461-20471 NN denotes conclusion
T8220 20458-20460 IN denotes In
T8219 20452-20456 NN denotes time
T8218 20446-20451 JJ denotes first
T8217 20442-20445 DT denotes the
T8216 20438-20441 IN denotes for
T8215 20431-20437 NN denotes injury
T8214 20423-20430 NN denotes scratch
T8213 20417-20422 IN denotes after
T8212 20406-20416 NN denotes astrocytes
T8211 20397-20405 NN denotes knockout
T8210 20392-20396 NN denotes Nrf2
T8209 20389-20391 IN denotes in
T8208 20384-20388 NN denotes MMP9
T8207 20380-20383 CC denotes and
T8206 20378-20379 -COMMA- denotes ,
T8205 20374-20378 NN denotes IL-6
T8204 20372-20373 -COMMA- denotes ,
T8203 20367-20372 NN denotes IL-1β
T8202 20365-20366 -COMMA- denotes ,
T8201 20360-20365 NN denotes TNF-α
T8200 20357-20359 IN denotes of
T8199 20346-20356 NN denotes expression
T8198 20334-20345 VB denotes upregulated
T8197 20330-20333 DT denotes the
T8196 20317-20329 VB denotes demonstrated
T8195 20309-20316 NN denotes results
T8194 20305-20308 PRP-DOLLAR- denotes Our
T8193 20300-20303 NN denotes TBI
T8192 20294-20299 IN denotes after
T8191 20284-20293 NN denotes mediators
T8190 20268-20283 JJ denotes proinflammatory
T8189 20264-20267 CC denotes and
T8188 20262-20263 -COMMA- denotes ,
T8187 20258-20262 NN denotes Nrf2
T8186 20256-20257 -COMMA- denotes ,
T8185 20246-20256 NN denotes astrocytes
T8184 20240-20245 IN denotes among
T8183 20227-20239 NN denotes relationship
T8182 20223-20226 DT denotes the
T8181 20220-20222 IN denotes on
T8180 20212-20219 VB denotes focused
T8179 20206-20211 NN denotes study
T8178 20203-20205 DT denotes no
T8177 20200-20202 VB denotes is
T8176 20194-20199 EX denotes there
T8175 20192-20193 -COMMA- denotes ,
T8174 20189-20192 RB denotes now
T8173 20184-20188 RB denotes till
T8172 20180-20183 CC denotes But
T8171 20172-20178 NN denotes injury
T8170 20166-20171 NN denotes brain
T8169 20160-20165 IN denotes after
T8168 20152-20159 NN denotes process
T8167 20139-20151 JJ denotes inflammatory
T8166 20136-20138 IN denotes in
T8165 20125-20135 NN denotes astrocytes
T8164 20122-20124 IN denotes of
T8163 20117-20121 NN denotes role
T8162 20113-20116 DT denotes the
T8161 20104-20112 VB denotes indicate
T8160 20096-20103 NN denotes results
T8159 20090-20095 DT denotes Those
T8158 20087-20088 -RRB- denotes ]
T8157 20085-20087 CD denotes 29
T8156 20084-20085 -LRB- denotes [
T8155 20063-20083 NN denotes immunohistochemistry
T8154 20060-20062 IN denotes by
T8153 20052-20059 NN denotes tissues
T8152 20046-20051 NN denotes brain
T8151 20043-20045 IN denotes in
T8150 20034-20042 VB denotes observed
T8149 20029-20033 VB denotes were
T8148 20018-20028 NN denotes astrocytes
T8147 20004-20017 JJ denotes MMP9-positive
T8146 20002-20003 -COMMA- denotes ,
T8145 19993-20002 NN denotes occlusion
T8144 19986-19992 NN denotes artery
T8143 19977-19985 JJ denotes cerebral
T8142 19970-19976 JJ denotes middle
T8141 19964-19969 IN denotes after
T8140 19959-19963 IN denotes that
T8139 19950-19958 VB denotes revealed
T8138 19944-19949 NN denotes study
T8137 19936-19943 DT denotes Another
T8136 19933-19934 -RRB- denotes ]
T8135 19931-19933 CD denotes 28
T8134 19929-19930 -COMMA- denotes ,
T8133 19927-19929 CD denotes 27
T8132 19926-19927 -LRB- denotes [
T8131 19912-19925 NN denotes oxyhemoglobin
T8130 19909-19911 CC denotes or
T8129 19905-19908 NN denotes LPS
T8128 19900-19904 IN denotes with
T8127 19890-19899 NN denotes treatment
T8126 19884-19889 IN denotes after
T8125 19873-19883 NN denotes astrocytes
T8124 19864-19872 VB denotes cultured
T8123 19861-19863 IN denotes in
T8122 19856-19860 NN denotes IL-6
T8121 19852-19855 CC denotes and
T8120 19850-19851 -COMMA- denotes ,
T8119 19845-19850 NN denotes IL-1β
T8118 19843-19844 -COMMA- denotes ,
T8117 19838-19843 NN denotes TNF-α
T8116 19835-19837 IN denotes of
T8115 19824-19834 NN denotes expression
T8114 19820-19823 DT denotes the
T8113 19809-19819 VB denotes identified
T8112 19803-19808 NN denotes study
T8111 19794-19802 JJ denotes Previous
T8110 19789-19792 NN denotes TBI
T8109 19783-19788 IN denotes after
T8108 19775-19782 NN denotes pathway
T8107 19766-19774 NN denotes Nrf2-ARE
T8049 19468-19469 -RRB- denotes )
T8048 19463-19468 NN denotes MAPKs
T8047 19462-19463 -LRB- denotes (
T8046 19455-19461 NN denotes kinase
T8045 19447-19454 NN denotes protein
T8044 19429-19446 JJ denotes mitogen-activated
T8043 19420-19428 JJ denotes upstream
T8042 19416-19419 DT denotes the
T8041 19413-19415 IN denotes by
T8040 19403-19412 VB denotes regulated
T8039 19400-19402 VB denotes be
T8038 19396-19399 MD denotes may
T8037 19390-19395 WDT denotes which
T8036 19388-19389 -COMMA- denotes ,
T8035 19384-19388 NN denotes Nrf2
T8034 19380-19383 CC denotes and
T8033 19374-19379 NN denotes NF-κB
T8032 19366-19373 IN denotes between
T8031 19356-19365 NN denotes crosstalk
T8030 19345-19355 NN denotes complicate
T8029 19335-19344 JJ denotes potential
T8028 19331-19334 DT denotes the
T8027 19322-19330 VB denotes indicate
T8026 19313-19321 NN denotes findings
T8025 19307-19312 DT denotes these
T8024 19303-19306 PDT denotes All
T8023 19300-19301 -RRB- denotes ]
T8022 19298-19300 CD denotes 25
T8021 19297-19298 -LRB- denotes [
T8020 19281-19296 NN denotes hypoacetylation
T8019 19273-19280 NN denotes histone
T8018 19267-19272 JJ denotes local
T8017 19264-19266 CC denotes or
T8016 19260-19263 NN denotes CBP
T8015 19257-19259 IN denotes of
T8014 19250-19256 NN denotes domain
T8013 19242-19249 NN denotes CH1-KIX
T8012 19238-19241 DT denotes the
T8011 19233-19237 IN denotes with
T8010 19221-19232 NN denotes interaction
T8009 19209-19220 JJ denotes competitive
T8008 19206-19208 IN denotes by
T8007 19200-19205 NN denotes level
T8006 19184-19199 JJ denotes transcriptional
T8005 19181-19183 IN denotes at
T8004 19173-19180 NN denotes pathway
T8003 19164-19172 NN denotes Nrf2-ARE
T8002 19160-19163 DT denotes the
T8001 19150-19159 VB denotes repressed
T8000 19142-19149 NN denotes subunit
T7999 19138-19141 NN denotes p65
T7998 19132-19137 NN denotes NF-κB
T7997 19125-19130 NN denotes level
T7996 19109-19124 JJ denotes transcriptional
T7995 19106-19108 IN denotes at
T7994 19101-19105 NN denotes Nrf2
T7993 19093-19100 VB denotes inhibit
T7992 19089-19092 MD denotes can
T7991 19083-19088 NN denotes NF-κB
T7990 19078-19082 IN denotes that
T7989 19069-19077 VB denotes reported
T7988 19066-19068 VB denotes is
T7987 19063-19065 PRP denotes it
T7986 19061-19062 -COMMA- denotes ,
T7985 19048-19061 RB denotes Interestingly
T7984 19045-19046 -RRB- denotes ]
T7983 19043-19045 CD denotes 24
T7982 19042-19043 -LRB- denotes [
T7981 19035-19041 NN denotes sepsis
T7980 19022-19034 JJ denotes experimental
T7979 19016-19021 IN denotes after
T7978 19011-19015 NN denotes mice
T7977 18996-19010 JJ denotes Nrf2-deficient
T7976 18993-18995 IN denotes of
T7975 18981-18992 NN denotes fibroblasts
T7974 18971-18980 JJ denotes embryonic
T7973 18965-18970 NN denotes mouse
T7972 18961-18964 CC denotes and
T7971 18959-18960 -COMMA- denotes ,
T7970 18948-18959 NN denotes macrophages
T7969 18946-18947 -COMMA- denotes ,
T7968 18942-18946 NN denotes lung
T7967 18939-18941 IN denotes in
T7966 18928-18938 VB denotes discovered
T7965 18923-18927 RB denotes also
T7964 18920-18922 VB denotes is
T7963 18914-18919 NN denotes NF-κB
T7962 18911-18913 IN denotes of
T7961 18900-18910 NN denotes activation
T7960 18891-18899 VB denotes Enhanced
T7959 18883-18889 NN denotes injury
T7958 18875-18882 NN denotes scratch
T7957 18869-18874 IN denotes after
T7956 18858-18868 NN denotes astrocytes
T7955 18849-18857 VB denotes cultured
T7954 18846-18848 IN denotes in
T7953 18838-18845 VB denotes existed
T7952 18825-18837 NN denotes relationship
T7951 18820-18824 JJ denotes such
T7950 18810-18819 VB denotes confirmed
T7949 18802-18809 RB denotes further
T7948 18796-18801 NN denotes study
T7947 18791-18795 DT denotes this
T7946 18786-18790 IN denotes from
T7945 18778-18785 NN denotes Results
T7944 18775-18776 -RRB- denotes ]
T7943 18773-18775 CD denotes 23
T7942 18771-18772 -COMMA- denotes ,
T7941 18770-18771 CD denotes 5
T7940 18768-18769 -COMMA- denotes ,
T7939 18767-18768 CD denotes 4
T7938 18766-18767 -LRB- denotes [
T7937 18762-18765 NNP denotes TBI
T7936 18756-18761 IN denotes after
T7935 18746-18755 NN denotes intestine
T7934 18742-18745 CC denotes and
T7933 18740-18741 -COMMA- denotes ,
T7932 18735-18740 NN denotes brain
T7931 18733-18734 -COMMA- denotes ,
T7930 18729-18733 NN denotes lung
T7929 18726-18728 IN denotes in
T7928 18717-18725 NN denotes response
T7927 18704-18716 JJ denotes inflammatory
T7926 18700-18703 CC denotes and
T7925 18691-18699 NN denotes activity
T7924 18685-18690 NN denotes NF-κB
T7923 18682-18684 IN denotes of
T7922 18669-18681 NN denotes augmentation
T7921 18661-18668 VB denotes induces
T7920 18656-18660 NN denotes Nrf2
T7919 18653-18655 IN denotes of
T7918 18643-18652 NN denotes depletion
T7917 18638-18642 IN denotes that
T7916 18629-18637 VB denotes indicate
T7915 18621-18628 NN denotes studies
T7914 18612-18620 JJ denotes previous
T7913 18608-18611 PRP-DOLLAR- denotes our
T7912 18606-18607 -COMMA- denotes ,
T7911 18595-18606 RB denotes Furthermore
T7910 18592-18593 -RRB- denotes ]
T7909 18590-18592 CD denotes 22
T7908 18589-18590 -LRB- denotes [
T7907 18587-18588 -RRB- denotes )
T7906 18584-18587 NN denotes CUR
T7905 18583-18584 -LRB- denotes (
T7904 18574-18582 NN denotes curcumin
T7903 18570-18573 CC denotes and
T7902 18566-18569 NN denotes SFN
T7901 18563-18565 IN denotes as
T7900 18558-18562 JJ denotes such
T7899 18556-18557 -COMMA- denotes ,
T7898 18546-18556 NN denotes activators
T7897 18541-18545 NN denotes Nrf2
T7896 18533-18540 JJ denotes diverse
T7895 18530-18532 IN denotes by
T7894 18519-18529 VB denotes attenuated
T7893 18516-18518 VB denotes be
T7892 18510-18515 MD denotes could
T7891 18508-18509 -RRB- denotes )
T7890 18505-18508 NN denotes LPS
T7889 18504-18505 -LRB- denotes (
T7888 18485-18503 NN denotes lipopolysaccharide
T7887 18482-18484 IN denotes by
T7886 18474-18481 VB denotes induced
T7885 18463-18473 NN denotes activation
T7884 18457-18462 NN denotes NF-κB
T7883 18453-18456 CC denotes And
T7882 18450-18451 -RRB- denotes ]
T7881 18448-18450 CD denotes 21
T7880 18447-18448 -LRB- denotes [
T7879 18437-18446 NN denotes signaling
T7878 18431-18436 NN denotes NF-κB
T7877 18419-18430 VB denotes alleviating
T7876 18406-18418 RB denotes consequently
T7875 18404-18405 -COMMA- denotes ,
T7874 18391-18404 NN denotes translocation
T7873 18383-18390 JJ denotes nuclear
T7872 18375-18382 NN denotes subunit
T7871 18369-18374 NN denotes NF-κB
T7870 18365-18368 NN denotes p65
T7869 18361-18364 CC denotes and
T7868 18345-18360 NN denotes phosphorylation
T7867 18337-18344 NN denotes IKK/IκB
T7866 18329-18336 VB denotes inhibit
T7865 18326-18328 TO denotes to
T7864 18321-18325 JJ denotes able
T7863 18318-18320 VB denotes be
T7862 18315-18317 TO denotes to
T7861 18309-18314 VB denotes found
T7860 18306-18308 VB denotes is
T7859 18302-18305 NN denotes SFN
T7858 18299-18301 IN denotes of
T7857 18284-18298 NN denotes Administration
T7856 18273-18282 NN denotes signaling
T7855 18267-18272 NN denotes NF-κB
T7854 18264-18266 IN denotes by
T7853 18255-18263 VB denotes mediated
T7852 18251-18254 VB denotes are
T7851 18245-18250 WDT denotes which
T7850 18236-18244 NN denotes pathways
T7849 18220-18235 JJ denotes proinflammatory
T7848 18217-18219 IN denotes of
T7847 18205-18216 NN denotes suppression
T7846 18202-18204 IN denotes by
T7845 18193-18201 VB denotes achieved
T7844 18190-18192 VB denotes be
T7843 18186-18189 MD denotes may
T7842 18181-18185 NN denotes Nrf2
T7841 18178-18180 IN denotes of
T7840 18171-18177 NN denotes effect
T7839 18159-18170 JJ denotes antioxidant
T7838 18154-18158 IN denotes that
T7837 18142-18153 NN denotes possibility
T7836 18138-18141 DT denotes the
T7835 18130-18137 VB denotes suggest
T7834 18122-18129 NN denotes studies
T7731 17529-17530 -LRB- denotes (
T7730 17512-17528 JJ denotes 4-Hydroxynonenal
T7729 17508-17511 IN denotes for
T7728 17491-17507 NN denotes immunoreactivity
T7727 17487-17490 DT denotes the
T7726 17477-17486 VB denotes decreases
T7725 17473-17476 CC denotes and
T7724 17469-17472 NN denotes rat
T7723 17466-17468 IN denotes of
T7722 17459-17465 NN denotes memory
T7721 17451-17458 JJ denotes spatial
T7720 17442-17450 VB denotes improves
T7719 17428-17441 RB denotes significantly
T7718 17426-17427 -COMMA- denotes ,
T7717 17422-17426 NN denotes Nrf2
T7716 17419-17421 IN denotes of
T7715 17411-17418 NN denotes inducer
T7714 17408-17410 DT denotes an
T7713 17406-17407 -COMMA- denotes ,
T7712 17403-17406 NNP denotes SFN
T7711 17400-17402 IN denotes of
T7710 17385-17399 NN denotes administration
T7709 17374-17384 NN denotes Postinjury
T7708 17371-17372 -RRB- denotes ]
T7707 17369-17371 CD denotes 18
T7706 17368-17369 -LRB- denotes [
T7705 17362-17367 NN denotes brain
T7704 17359-17361 IN denotes in
T7703 17352-17358 NN denotes damage
T7702 17348-17351 DT denotes the
T7701 17341-17347 VB denotes reduce
T7700 17328-17340 RB denotes subsequently
T7699 17322-17327 MD denotes would
T7698 17316-17321 WDT denotes which
T7697 17314-17315 -COMMA- denotes ,
T7696 17313-17314 -RRB- denotes )
T7695 17309-17313 NN denotes NQO1
T7694 17308-17309 -LRB- denotes (
T7693 17291-17307 NN denotes oxidoreductase-1
T7692 17283-17290 NN denotes quinone
T7691 17281-17282 -COLON- denotes :
T7690 17280-17281 NN denotes H
T7689 17279-17280 -RRB- denotes )
T7688 17278-17279 NN denotes P
T7687 17277-17278 -LRB- denotes (
T7686 17273-17276 NN denotes NAD
T7685 17269-17272 CC denotes and
T7684 17267-17268 -RRB- denotes )
T7683 17263-17267 NN denotes HO-1
T7682 17262-17263 -LRB- denotes (
T7681 17250-17261 NN denotes oxygenase-1
T7680 17245-17249 NN denotes heme
T7679 17242-17244 IN denotes as
T7678 17237-17241 JJ denotes such
T7677 17235-17236 -COMMA- denotes ,
T7676 17230-17235 NN denotes genes
T7675 17218-17229 JJ denotes antioxidant
T7674 17215-17217 IN denotes of
T7673 17209-17214 NN denotes group
T7672 17207-17208 DT denotes a
T7671 17204-17206 IN denotes of
T7670 17190-17203 NN denotes transcription
T7669 17180-17189 VB denotes activates
T7668 17175-17179 NN denotes Nrf2
T7667 17172-17174 IN denotes of
T7666 17166-17171 NN denotes level
T7665 17157-17165 VB denotes Enhanced
T7664 17154-17155 -RRB- denotes ]
T7663 17152-17154 CD denotes 17
T7662 17151-17152 -LRB- denotes [
T7661 17147-17150 NNP denotes TBI
T7660 17141-17146 IN denotes after
T7659 17134-17140 NN denotes tissue
T7658 17128-17133 NN denotes brain
T7657 17125-17127 IN denotes in
T7656 17120-17124 NN denotes Nrf2
T7655 17117-17119 IN denotes of
T7654 17104-17116 NN denotes augmentation
T7653 17100-17103 DT denotes the
T7652 17093-17099 VB denotes proved
T7651 17089-17092 VB denotes has
T7650 17083-17088 NN denotes study
T7649 17074-17082 JJ denotes previous
T7648 17070-17073 PRP-DOLLAR- denotes Our
T7647 17065-17068 NNP denotes TBI
T7646 17062-17064 IN denotes in
T7645 17053-17061 VB denotes involved
T7644 17049-17052 DT denotes all
T7643 17045-17048 VB denotes are
T7642 17039-17044 WDT denotes which
T7641 17037-17038 -COMMA- denotes ,
T7640 17026-17037 NN denotes metabolites
T7639 17020-17025 JJ denotes toxic
T7638 17017-17019 IN denotes of
T7637 17004-17016 NN denotes accumulation
T7636 17000-17003 CC denotes and
T7635 16998-16999 -COMMA- denotes ,
T7634 16992-16998 NN denotes damage
T7633 16979-16991 JJ denotes inflammatory
T7632 16977-16978 -COMMA- denotes ,
T7631 16971-16977 NN denotes stress
T7630 16961-16970 JJ denotes oxidative
T7629 16952-16960 VB denotes reducing
T7628 16949-16951 IN denotes in
T7627 16939-16948 NN denotes regulator
T7626 16935-16938 JJ denotes key
T7625 16931-16934 DT denotes the
T7624 16928-16930 VB denotes be
T7623 16925-16927 TO denotes to
T7622 16918-16924 VB denotes proved
T7621 16913-16917 VB denotes been
T7620 16909-16912 VB denotes has
T7619 16901-16908 NN denotes pathway
T7618 16892-16900 NN denotes Nrf2-ARE
T7617 16887-16890 NN denotes TBI
T7616 16881-16886 IN denotes after
T7615 16876-16880 NN denotes loop
T7614 16861-16875 JJ denotes autoregulatory
T7613 16856-16860 JJ denotes such
T7612 16848-16855 VB denotes prevent
T7611 16845-16847 TO denotes to
T7610 16838-16844 NN denotes factor
T7609 16829-16837 JJ denotes upstream
T7608 16826-16828 DT denotes an
T7607 16823-16825 IN denotes on
T7606 16813-16822 VB denotes targeting
T7605 16809-16812 IN denotes for
T7604 16802-16808 NN denotes choice
T7603 16797-16801 JJ denotes good
T7602 16795-16796 DT denotes a
T7601 16792-16794 VB denotes is
T7600 16789-16791 PRP denotes It
T7599 16782-16787 NN denotes brain
T7598 16779-16781 TO denotes to
T7597 16772-16778 NN denotes injury
T7596 16762-16771 JJ denotes secondary
T7595 16754-16761 VB denotes induces
T7594 16750-16753 CC denotes and
T7593 16737-16749 NN denotes inflammation
T7592 16734-16736 IN denotes of
T7591 16727-16733 NN denotes effect
T7590 16718-16726 JJ denotes damaging
T7589 16714-16717 DT denotes the
T7588 16703-16713 VB denotes aggravates
T7587 16693-16702 RB denotes extremely
T7586 16688-16692 NN denotes loop
T7585 16673-16687 JJ denotes autoregulatory
T7584 16668-16672 DT denotes This
T7583 16665-16666 -RRB- denotes ]
T7582 16663-16665 CD denotes 16
T7581 16661-16662 -COMMA- denotes ,
T7580 16659-16661 CD denotes 15
T7579 16658-16659 -LRB- denotes [
T7578 16652-16657 NN denotes NF-κB
T7577 16649-16651 IN denotes of
T7576 16638-16648 NN denotes activation
T7575 16631-16637 VB denotes induce
T7574 16627-16630 MD denotes can
T7573 16622-16626 RB denotes also
T7572 16616-16621 NN denotes IL-1β
T7571 16612-16615 CC denotes and
T7570 16606-16611 NN denotes TNF-α
T7569 16602-16605 CC denotes and
T7568 16600-16601 -COMMA- denotes ,
T7567 16599-16600 -RRB- denotes ]
T7566 16597-16599 CD denotes 14
T7565 16596-16597 -LRB- denotes [
T7564 16590-16595 NN denotes cells
T7563 16580-16589 NN denotes carcinoma
T7562 16574-16579 NN denotes colon
T7561 16568-16573 JJ denotes human
T7560 16565-16567 IN denotes in
T7559 16560-16564 NN denotes MMP9
T7558 16557-16559 IN denotes of
T7557 16542-16556 NN denotes overexpression
T7556 16534-16541 VB denotes induces
T7555 16529-16533 NN denotes IL-6
T7554 16525-16528 CC denotes and
T7553 16523-16524 -COMMA- denotes ,
T7552 16522-16523 -RRB- denotes ]
T7551 16520-16522 CD denotes 13
T7550 16518-16519 -COMMA- denotes ,
T7549 16516-16518 CD denotes 12
T7548 16515-16516 -LRB- denotes [
T7547 16504-16514 NN denotes astrocytes
T7546 16501-16503 IN denotes in
T7545 16496-16500 NN denotes MMP9
T7544 16492-16495 IN denotes for
T7543 16480-16491 NN denotes stimulators
T7542 16473-16479 JJ denotes potent
T8106 19763-19765 IN denotes of
T8105 19752-19762 NN denotes activation
T8104 19749-19751 IN denotes in
T8103 19743-19748 NN denotes cells
T8102 19732-19742 NN denotes respondent
T8101 19729-19731 IN denotes of
T8100 19724-19728 NN denotes kind
T8099 19720-19723 CD denotes one
T8098 19717-19719 VB denotes be
T8097 19713-19716 MD denotes may
T8096 19702-19712 NN denotes astrocytes
T8095 19697-19701 IN denotes that
T8094 19688-19696 VB denotes reasoned
T8093 19685-19687 VB denotes be
T8092 19681-19684 MD denotes can
T8091 19678-19680 PRP denotes it
T8090 19676-19677 -COMMA- denotes ,
T8089 19669-19676 NN denotes nucleus
T8088 19666-19668 IN denotes in
T8087 19659-19665 RB denotes mainly
T8086 19650-19658 NN denotes function
T8085 19645-19649 VB denotes take
T8084 19638-19644 MD denotes should
T8083 19632-19637 WDT denotes which
T8082 19625-19631 NN denotes factor
T8081 19611-19624 NN denotes transcription
T8080 19609-19610 DT denotes a
T8079 19606-19608 VB denotes is
T8078 19601-19605 NN denotes Nrf2
T8077 19596-19600 IN denotes that
T8076 19591-19595 NN denotes fact
T8075 19587-19590 DT denotes the
T8074 19584-19586 IN denotes of
T8073 19579-19583 NN denotes view
T8072 19576-19578 IN denotes In
T8071 19573-19574 -RRB- denotes ]
T8070 19571-19573 CD denotes 18
T8069 19570-19571 -LRB- denotes [
T8068 19566-19569 NNP denotes TBI
T8067 19560-19565 IN denotes after
T8066 19549-19559 NN denotes astrocytes
T8065 19546-19548 IN denotes of
T8064 19538-19545 NN denotes nucleus
T8063 19535-19537 IN denotes in
T8062 19526-19534 VB denotes detected
T8061 19519-19525 RB denotes mainly
T8060 19516-19518 VB denotes is
T8059 19511-19515 NN denotes Nrf2
T8058 19506-19510 IN denotes that
T8057 19496-19505 VB denotes confirmed
T8056 19491-19495 VB denotes been
T8055 19487-19490 VB denotes has
T8054 19484-19486 PRP denotes It
T8053 19481-19482 -RRB- denotes ]
T8052 19479-19481 CD denotes 26
T8051 19478-19479 -LRB- denotes [
T8050 19470-19477 NN denotes pathway
T7541 16469-16472 VB denotes are
T7540 16463-16468 NN denotes IL-1β
T7539 16459-16462 CC denotes and
T7538 16453-16458 NN denotes TNF-α
T7537 16451-16452 -COMMA- denotes ,
T7536 16444-16451 NN denotes example
T7535 16440-16443 IN denotes For
T7534 16429-16438 NN denotes mediators
T7533 16413-16428 JJ denotes proinflammatory
T7532 16407-16412 DT denotes these
T7531 16401-16406 IN denotes among
T7530 16396-16400 NN denotes loop
T7529 16381-16395 JJ denotes autoregulatory
T7528 16378-16380 DT denotes an
T7527 16375-16377 VB denotes is
T7526 16369-16374 EX denotes there
T7525 16364-16368 IN denotes that
T7524 16351-16363 VB denotes demonstrated
T7523 16346-16350 VB denotes been
T7522 16342-16345 VB denotes has
T7521 16339-16341 PRP denotes It
T7520 16327-16337 NN denotes astrocytes
T7519 16318-16326 VB denotes cultured
T7518 16315-16317 IN denotes in
T7517 16309-16314 IN denotes above
T7516 16299-16308 VB denotes mentioned
T7410 15695-15705 NN denotes expression
T7409 15686-15694 VB denotes enhanced
T7408 15682-15685 CC denotes and
T7407 15676-15681 NN denotes NF-κB
T7406 15673-15675 IN denotes of
T7405 15664-15672 NN denotes activity
T7404 15657-15663 JJ denotes higher
T7403 15649-15656 VB denotes induced
T7402 15642-15648 NN denotes injury
T7401 15634-15641 NN denotes scratch
T7400 15629-15633 IN denotes that
T7399 15620-15628 VB denotes revealed
T7398 15617-15619 PRP denotes we
T7397 15612-15616 RB denotes Also
T7396 15600-15610 NN denotes astrocytes
T7395 15591-15599 VB denotes cultured
T7394 15588-15590 IN denotes in
T7393 15583-15587 NN denotes MMP9
T7392 15579-15582 CC denotes and
T7391 15577-15578 -COMMA- denotes ,
T7390 15573-15577 NN denotes IL-6
T7389 15571-15572 -COMMA- denotes ,
T7388 15566-15571 NN denotes IL-1β
T7387 15564-15565 -COMMA- denotes ,
T7386 15559-15564 NN denotes TNF-α
T7385 15556-15558 IN denotes of
T7384 15541-15555 NN denotes overexpression
T7383 15537-15540 CC denotes and
T7382 15528-15536 NN denotes activity
T7381 15520-15527 NN denotes binding
T7380 15516-15519 NN denotes DNA
T7379 15510-15515 NN denotes NF-κB
T7378 15507-15509 IN denotes of
T7377 15494-15506 NN denotes upregulation
T7376 15490-15493 DT denotes the
T7375 15482-15489 VB denotes induced
T7374 15475-15481 NN denotes injury
T7373 15467-15474 NN denotes scratch
T7372 15462-15466 IN denotes that
T7371 15449-15461 VB denotes demonstrated
T7370 15443-15448 NN denotes study
T7369 15435-15442 JJ denotes present
T7368 15431-15434 DT denotes The
T7833 18115-18121 JJ denotes animal
T7832 18110-18114 IN denotes from
T7831 18101-18109 VB denotes obtained
T7830 18096-18100 NN denotes Data
T7829 18088-18094 NN denotes deaths
T7828 18077-18087 NN denotes astrocytes
T7827 18072-18076 JJ denotes more
T7826 18069-18071 TO denotes to
T7825 18065-18068 VB denotes led
T7824 18055-18064 NN denotes mediators
T7823 18039-18054 JJ denotes proinflammatory
T7822 18033-18038 DT denotes these
T7821 18030-18032 IN denotes of
T7820 18015-18029 NN denotes overexpression
T7819 18011-18014 CC denotes and
T7818 18009-18010 -COMMA- denotes ,
T7817 18005-18009 NN denotes time
T7816 17999-18004 JJ denotes first
T7815 17995-17998 DT denotes the
T7814 17991-17994 IN denotes for
T7813 17979-17990 NN denotes astrocyetes
T7812 17970-17978 JJ denotes wildtype
T7811 17967-17969 IN denotes in
T7810 17962-17966 IN denotes than
T7809 17951-17961 NN denotes astrocytes
T7808 17942-17950 NN denotes knockout
T7807 17937-17941 NN denotes Nrf2
T7806 17928-17936 VB denotes cultured
T7805 17925-17927 IN denotes in
T7804 17914-17924 VB denotes aggravated
T7803 17909-17913 RB denotes more
T7802 17905-17908 VB denotes was
T7801 17898-17904 NN denotes injury
T7800 17890-17897 NN denotes scratch
T7799 17884-17889 IN denotes after
T7798 17879-17883 NN denotes MMP9
T7797 17875-17878 CC denotes and
T7796 17873-17874 -COMMA- denotes ,
T7795 17869-17873 NN denotes IL-6
T7794 17867-17868 -COMMA- denotes ,
T7793 17862-17867 NN denotes IL-1β
T7792 17860-17861 -COMMA- denotes ,
T7791 17855-17860 NN denotes TNF-α
T7790 17852-17854 IN denotes of
T7789 17837-17851 NN denotes overexpression
T7788 17832-17836 IN denotes that
T7787 17823-17831 VB denotes revealed
T7786 17815-17822 NN denotes results
T7785 17811-17814 PRP-DOLLAR- denotes our
T7784 17809-17810 -COMMA- denotes ,
T7783 17805-17809 RB denotes Here
T7782 17802-17803 -RRB- denotes ]
T7781 17800-17802 CD denotes 20
T7780 17799-17800 -LRB- denotes [
T7779 17795-17798 NNP denotes TBI
T7778 17789-17794 IN denotes after
T7777 17783-17788 NN denotes brain
T7776 17780-17782 IN denotes in
T7775 17770-17779 NN denotes mediators
T7774 17754-17769 JJ denotes proinflammatory
T7773 17751-17753 IN denotes of
T7772 17740-17750 NN denotes expression
T7771 17733-17739 JJ denotes higher
T7770 17725-17732 VB denotes induces
T7769 17720-17724 NN denotes Nrf2
T7768 17717-17719 IN denotes of
T7767 17707-17716 NN denotes Depletion
T7766 17702-17705 NNP denotes TBI
T7765 17699-17701 TO denotes to
T7764 17687-17698 JJ denotes susceptible
T7763 17682-17686 RB denotes more
T7762 17675-17681 VB denotes appear
T7761 17670-17674 NN denotes mice
T7760 17655-17669 JJ denotes Nrf2-deficient
T7759 17653-17654 -COMMA- denotes ,
T7758 17649-17653 NN denotes hand
T7757 17643-17648 JJ denotes other
T7756 17639-17642 DT denotes the
T7755 17636-17638 IN denotes On
T7754 17633-17634 -RRB- denotes ]
T7753 17631-17633 CD denotes 19
T7752 17630-17631 -LRB- denotes [
T7751 17626-17629 NNP denotes TBI
T7750 17620-17625 IN denotes after
T7749 17608-17619 NN denotes hippocampus
T7748 17605-17607 IN denotes of
T7747 17596-17604 NN denotes subfield
T7746 17592-17595 NN denotes CA3
T7745 17588-17591 DT denotes the
T7744 17584-17587 CC denotes and
T7743 17577-17583 NN denotes cortex
T7742 17573-17576 DT denotes the
T7741 17570-17572 IN denotes in
T7740 17568-17569 -COMMA- denotes ,
T7739 17556-17568 NN denotes peroxidation
T7738 17550-17555 NN denotes lipid
T7737 17547-17549 IN denotes of
T7736 17540-17546 NN denotes marker
T7735 17538-17539 DT denotes a
T7734 17536-17537 -COMMA- denotes ,
T7733 17535-17536 -RRB- denotes )
T7732 17530-17535 NN denotes 4-HNE
T7515 16289-16298 NN denotes mediators
T7514 16273-16288 JJ denotes proinflammatory
T7513 16269-16272 CC denotes and
T7512 16267-16268 -COMMA- denotes ,
T7511 16263-16267 NN denotes MMP9
T7510 16260-16262 IN denotes of
T7509 16245-16259 NN denotes overexpression
T7508 16243-16244 -COMMA- denotes ,
T7507 16235-16243 NN denotes activity
T7506 16227-16234 NN denotes binding
T7505 16223-16226 NN denotes DNA
T7504 16217-16222 NN denotes NF-κB
T7503 16214-16216 IN denotes of
T7502 16204-16213 NN denotes elevation
T7501 16196-16203 VB denotes induced
T7500 16189-16195 NN denotes injury
T7499 16181-16188 NN denotes scratch
T7498 16176-16180 IN denotes that
T7497 16167-16175 VB denotes revealed
T7496 16162-16166 RB denotes also
T7495 16154-16161 NN denotes results
T7494 16150-16153 PRP-DOLLAR- denotes Our
T7493 16147-16148 -RRB- denotes ]
T7492 16145-16147 CD denotes 11
T7491 16143-16144 -COMMA- denotes ,
T7490 16141-16143 CD denotes 10
T7489 16139-16140 -COMMA- denotes ,
T7488 16138-16139 CD denotes 3
T7487 16137-16138 -LRB- denotes [
T7486 16133-16136 NNP denotes TBI
T7485 16127-16132 IN denotes after
T7484 16119-16126 NN denotes tissues
T7483 16113-16118 NN denotes brain
T7482 16110-16112 IN denotes in
T7481 16108-16109 -COMMA- denotes ,
T7480 16104-16108 NN denotes IL-6
T7479 16100-16103 CC denotes and
T7478 16098-16099 -COMMA- denotes ,
T7477 16093-16098 NN denotes IL-1β
T7476 16091-16092 -COMMA- denotes ,
T7475 16086-16091 NN denotes TNF-α
T7474 16076-16085 VB denotes including
T7473 16074-16075 -COMMA- denotes ,
T7472 16065-16074 NN denotes cytokines
T7471 16049-16064 JJ denotes proinflammatory
T7470 16046-16048 IN denotes of
T7469 16038-16045 NN denotes battery
T7468 16036-16037 DT denotes a
T7467 16032-16035 CC denotes and
T7466 16027-16031 NN denotes MMP9
T7465 16024-16026 IN denotes of
T7464 16010-16023 NN denotes transcription
T7463 16002-16009 VB denotes induces
T7462 15998-16001 CC denotes and
T7461 15988-15997 VB denotes activated
T7460 15984-15987 VB denotes was
T7459 15981-15983 PRP denotes it
T7458 15976-15980 IN denotes once
T7457 15972-15975 NN denotes DNA
T7456 15967-15971 IN denotes with
T7455 15961-15966 VB denotes binds
T7454 15955-15960 NN denotes NF-κB
T7453 15953-15954 -COMMA- denotes ,
T7452 15947-15953 NN denotes factor
T7451 15936-15946 NN denotes transcript
T7450 15934-15935 DT denotes a
T7449 15931-15933 IN denotes As
T7448 15928-15929 -RRB- denotes ]
T7447 15927-15928 CD denotes 9
T7446 15925-15926 -COMMA- denotes ,
T7445 15924-15925 CD denotes 2
T7444 15923-15924 -LRB- denotes [
T7443 15916-15922 NN denotes injury
T7442 15906-15915 JJ denotes traumatic
T7441 15900-15905 IN denotes after
T7440 15895-15899 NN denotes cord
T7439 15888-15894 JJ denotes spinal
T7438 15884-15887 CC denotes and
T7437 15878-15883 NN denotes brain
T7436 15875-15877 IN denotes in
T7435 15865-15874 VB denotes activated
T7434 15862-15864 VB denotes is
T7433 15856-15861 NN denotes NF-κB
T7432 15851-15855 IN denotes that
T7431 15838-15850 VB denotes demonstrated
T7430 15833-15837 VB denotes been
T7429 15829-15832 VB denotes has
T7428 15826-15828 PRP denotes It
T7427 15820-15824 NN denotes time
T7426 15814-15819 JJ denotes first
T7425 15810-15813 DT denotes the
T7424 15806-15809 IN denotes for
T7423 15795-15805 NN denotes astrocytes
T7422 15786-15794 JJ denotes wildtype
T7421 15783-15785 IN denotes in
T7420 15777-15782 DT denotes those
T7419 15772-15776 IN denotes than
T7418 15761-15771 NN denotes astrocytes
T7417 15752-15760 VB denotes cultured
T7416 15743-15751 NN denotes knockout
T7415 15738-15742 NN denotes Nrf2
T7414 15735-15737 IN denotes in
T7413 15725-15734 NN denotes cytokines
T7412 15709-15724 JJ denotes proinflammatory
T7411 15706-15708 IN denotes of
T6696 15413-15414 -RRB- denotes )
T6695 15412-15413 CD denotes 6
T6694 15405-15411 NN denotes Figure
T6693 15404-15405 -LRB- denotes (
T6692 15402-15403 -RRB- denotes )
T6691 15398-15402 CD denotes 0.01
T6690 15396-15397 SYM denotes <
T6689 15394-15395 NN denotes P
T6688 15392-15393 -COMMA- denotes ,
T6687 15387-15392 CD denotes 3.388
T6686 15385-15386 SYM denotes ±
T6685 15379-15384 CD denotes 76.32
T6684 15372-15378 IN denotes versus
T6683 15366-15371 CD denotes 6.343
T6682 15364-15365 SYM denotes ±
T6681 15357-15363 CD denotes 101.76
T6680 15356-15357 -LRB- denotes (
T6679 15352-15355 RB denotes too
T6678 15348-15351 NNP denotes TBI
T6677 15345-15347 NNP denotes WT
T6676 15339-15344 NN denotes group
T6675 15336-15338 IN denotes in
T6674 15331-15335 DT denotes that
T6673 15326-15330 IN denotes than
T6672 15322-15325 NN denotes TBI
T6671 15319-15321 NN denotes KO
T6670 15313-15318 NN denotes group
T6654 15237-15238 -LRB- denotes (
T6653 15233-15236 NN denotes TBI
T6652 15230-15232 NN denotes WT
T6651 15224-15229 NN denotes group
T6650 15220-15223 IN denotes for
T6649 15214-15219 CD denotes 0.048
T6648 15212-15213 SYM denotes ±
T6647 15207-15211 CD denotes 1.13
T6646 15203-15206 CC denotes and
T6645 15199-15202 NNP denotes TBI
T6644 15196-15198 NN denotes KO
T6643 15190-15195 NN denotes group
T6642 15186-15189 IN denotes for
T6641 15180-15185 CD denotes 0.076
T6640 15178-15179 SYM denotes ±
T6639 15173-15177 CD denotes 1.44
T6638 15170-15172 IN denotes as
T6637 15168-15169 -COMMA- denotes ,
T6636 15164-15168 NN denotes blot
T6635 15156-15163 JJ denotes western
T6634 15153-15155 IN denotes by
T6633 15147-15152 NN denotes level
T6632 15139-15146 NN denotes protein
T6631 15134-15138 NN denotes MMP9
T6630 15131-15133 IN denotes in
T6629 15120-15130 VB denotes discovered
T6628 15115-15119 RB denotes also
T6627 15111-15114 VB denotes was
T6626 15100-15110 NN denotes difference
T6625 15088-15099 JJ denotes Significant
T6624 15085-15086 -RRB- denotes )
T6623 15084-15085 -RRB- denotes )
T6622 15083-15084 NN denotes e
T6621 15082-15083 -LRB- denotes (
T6620 15081-15082 CD denotes 3
T6619 15077-15080 CC denotes and
T6618 15075-15076 -RRB- denotes )
T6617 15074-15075 DT denotes a
T6616 15073-15074 -LRB- denotes (
T6615 15072-15073 CD denotes 3
T6614 15064-15071 NN denotes Figures
T6613 15063-15064 -LRB- denotes (
T6612 15061-15062 -RRB- denotes )
T6611 15057-15061 CD denotes 0.01
T6610 15055-15056 SYM denotes <
T6609 15053-15054 NN denotes P
T6608 15051-15052 -COMMA- denotes ,
T6607 15046-15051 CD denotes 0.068
T6606 15044-15045 SYM denotes ±
T6602 15024-15025 SYM denotes ±
T6601 15019-15023 CD denotes 1.36
T6600 15018-15019 -LRB- denotes (
T6599 15014-15017 NN denotes TBI
T6598 15011-15013 NN denotes WT
T6597 15005-15010 NN denotes group
T6596 15002-15004 IN denotes in
T6595 14997-15001 IN denotes than
T6594 14993-14996 NN denotes TBI
T6593 14990-14992 NN denotes KO
T6592 14984-14989 NN denotes group
T6591 14981-14983 IN denotes in
T6590 14974-14980 JJ denotes higher
T6589 14970-14973 VB denotes was
T6588 14965-14969 NN denotes MMP9
T6587 14962-14964 IN denotes of
T6586 14956-14961 NN denotes level
T6585 14951-14955 NN denotes mRNA
T6584 14947-14950 DT denotes The
T6583 14933-14945 NN denotes counterparts
T6582 14928-14932 NN denotes sham
T6581 14922-14927 PRP-DOLLAR- denotes their
T6580 14917-14921 IN denotes with
T6579 14908-14916 VB denotes compared
T6578 14905-14907 IN denotes as
T6577 14894-14904 NN denotes astrocytes
T6576 14891-14893 NN denotes KO
T6575 14887-14890 CC denotes and
T6574 14884-14886 NN denotes WT
T6573 14881-14883 IN denotes in
T6572 14874-14880 NN denotes injury
T6571 14866-14873 NN denotes scratch
T6570 14860-14865 IN denotes after
T6569 14851-14859 VB denotes elevated
T6568 14847-14850 VB denotes was
T6567 14842-14846 NN denotes MMP9
T6566 14839-14841 IN denotes of
T6565 14828-14838 NN denotes Expression
T6564 14816-14826 NN denotes zymography
T6563 14807-14815 NN denotes gelatine
T6562 14803-14806 CC denotes and
T6561 14801-14802 -COMMA- denotes ,
T6560 14797-14801 NN denotes blot
T6559 14789-14796 JJ denotes western
T6558 14787-14788 -COMMA- denotes ,
T6557 14781-14787 NN denotes RT-PCR
T6556 14778-14780 IN denotes by
T6555 14771-14777 NN denotes injury
T6554 14763-14770 NN denotes scratch
T6553 14757-14762 IN denotes after
T6552 14755-14756 NN denotes h
T6551 14752-14754 CD denotes 24
T6550 14749-14751 IN denotes at
T6549 14738-14748 NN denotes astrocytes
T6548 14735-14737 IN denotes in
T6547 14730-14734 NN denotes MMP9
T6546 14727-14729 IN denotes of
T6545 14718-14726 NN denotes activity
T6544 14714-14717 CC denotes and
T6543 14712-14713 -COMMA- denotes ,
T6542 14706-14712 NN denotes levels
T6541 14698-14705 NN denotes protein
T6540 14696-14697 -COMMA- denotes ,
T6539 14692-14696 NN denotes mRNA
T6538 14688-14691 DT denotes the
T6537 14679-14687 VB denotes elevated
T6536 14676-14678 PRP denotes we
T6535 14674-14675 -COMMA- denotes ,
T6534 14669-14674 NN denotes study
T6533 14661-14668 JJ denotes present
T6532 14657-14660 DT denotes the
T6531 14654-14656 IN denotes In
T6530 14645-14652 NN denotes process
T6529 14632-14644 NN denotes inflammation
T6528 14628-14631 DT denotes the
T6527 14618-14627 VB denotes aggravate
T6526 14615-14617 CC denotes or
T6525 14608-14614 VB denotes induce
T6524 14605-14607 TO denotes to
T6523 14594-14604 NN denotes gelatinase
T6522 14584-14593 JJ denotes important
T6521 14581-14583 DT denotes an
T6520 14578-14580 VB denotes is
T6519 14573-14577 NN denotes MMP9
T6518 14566-14572 NNP denotes Injury
T6517 14558-14565 NNP denotes Scratch
T6516 14552-14557 IN denotes after
T6515 14541-14551 NN denotes Astrocytes
T6514 14532-14540 NNP denotes Knockout
T6513 14527-14531 NN denotes Nrf2
T6512 14524-14526 IN denotes in
T6511 14515-14523 VB denotes Enhanced
T6510 14507-14514 RB denotes Greatly
T6509 14502-14506 VB denotes Were
T6508 14497-14501 NN denotes MMP9
T6507 14494-14496 IN denotes of
T6506 14485-14493 NN denotes Activity
T6505 14481-14484 CC denotes and
T6504 14470-14480 NN denotes Expression
T6503 14465-14468 CD denotes 3.4
T5826 14461-14462 -RRB- denotes )
T5825 14460-14461 -RRB- denotes )
T5824 14459-14460 NN denotes c
T5823 14458-14459 -LRB- denotes (
T5822 14457-14458 CD denotes 4
T5821 14450-14456 NN denotes Figure
T5820 14449-14450 -LRB- denotes (
T5819 14447-14448 -RRB- denotes )
T5818 14443-14447 CD denotes 0.01
T5817 14441-14442 SYM denotes <
T5816 14439-14440 NN denotes P
T5815 14438-14439 -LRB- denotes (
T5814 14434-14437 NN denotes TBI
T5813 14431-14433 NN denotes WT
T5812 14425-14430 NN denotes group
T5811 14421-14424 IN denotes for
T5810 14415-14420 CD denotes 5.348
T5809 14413-14414 SYM denotes ±
T5808 14406-14412 CD denotes 154.50
T5807 14400-14405 IN denotes while
T5806 14396-14399 NNP denotes TBI
T5805 14393-14395 NNP denotes KO
T5804 14387-14392 NN denotes group
T5803 14383-14386 IN denotes for
T5802 14377-14382 CD denotes 6.339
T5801 14375-14376 SYM denotes ±
T5800 14368-14374 CD denotes 190.75
T5799 14364-14367 VB denotes was
T5798 14361-14363 PRP denotes it
T5797 14359-14360 -COMMA- denotes ,
T5796 14354-14359 NN denotes IL-1β
T5795 14350-14353 IN denotes For
T5794 14347-14348 -RRB- denotes )
T5793 14346-14347 -RRB- denotes )
T5792 14345-14346 NN denotes b
T5791 14344-14345 -LRB- denotes (
T5790 14343-14344 CD denotes 4
T5789 14336-14342 NN denotes Figure
T5788 14335-14336 -LRB- denotes (
T5787 14333-14334 -RRB- denotes )
T5786 14329-14333 CD denotes 0.01
T5785 14327-14328 SYM denotes <
T5784 14325-14326 NN denotes P
T5783 14324-14325 -LRB- denotes (
T5782 14320-14323 NN denotes TBI
T5781 14317-14319 NN denotes WT
T5780 14311-14316 NN denotes group
T5779 14307-14310 IN denotes for
T5778 14301-14306 CD denotes 0.536
T5777 14299-14300 SYM denotes ±
T5776 14293-14298 CD denotes 25.76
T5775 14287-14292 IN denotes while
T5774 14283-14286 NNP denotes TBI
T5773 14280-14282 NNP denotes KO
T5772 14274-14279 NN denotes group
T5771 14270-14273 IN denotes for
T5770 14264-14269 CD denotes 0.786
T5769 14262-14263 SYM denotes ±
T5768 14256-14261 CD denotes 36.07
T5767 14252-14255 VB denotes was
T5766 14249-14251 PRP denotes it
T5765 14247-14248 -COMMA- denotes ,
T5764 14243-14247 NN denotes IL-6
T5763 14239-14242 IN denotes For
T5762 14232-14237 NN denotes IL-1β
T5761 14228-14231 CC denotes and
T5760 14223-14227 NN denotes IL-6
T5759 14220-14222 IN denotes of
T5758 14214-14219 NN denotes level
T5757 14206-14213 NN denotes protein
T5756 14203-14205 IN denotes in
T6669 15310-15312 IN denotes in
T6668 15301-15309 NN denotes activity
T6667 15296-15300 NN denotes MMP9
T6666 15289-15295 JJ denotes higher
T6665 15280-15288 VB denotes revealed
T6664 15269-15279 NN denotes zymography
T6663 15260-15268 NN denotes Gelatine
T6662 15257-15258 -RRB- denotes )
T6661 15256-15257 CD denotes 5
T6660 15249-15255 NN denotes Figure
T6659 15248-15249 -LRB- denotes (
T6658 15246-15247 -RRB- denotes )
T6657 15242-15246 CD denotes 0.01
T6656 15240-15241 SYM denotes <
T6655 15238-15239 NN denotes P
T5755 14194-14202 VB denotes observed
T5754 14190-14193 VB denotes was
T5753 14181-14189 NN denotes tendency
T5752 14173-14180 JJ denotes Similar
T5751 14170-14171 -RRB- denotes )
T5750 14169-14170 -RRB- denotes )
T5749 14168-14169 DT denotes a
T5748 14167-14168 -LRB- denotes (
T5747 14166-14167 CD denotes 4
T5746 14159-14165 NN denotes Figure
T5745 14158-14159 -LRB- denotes (
T5744 14156-14157 -RRB- denotes )
T5743 14152-14156 CD denotes 0.01
T5742 14150-14151 SYM denotes <
T5741 14148-14149 NN denotes P
T5740 14146-14147 -COMMA- denotes ,
T5739 14141-14146 CD denotes 0.087
T5738 14139-14140 SYM denotes ±
T5737 14134-14138 CD denotes 2.31
T5736 14127-14133 IN denotes versus
T5735 14121-14126 CD denotes 0.156
T5734 14119-14120 SYM denotes ±
T5733 14114-14118 CD denotes 3.67
T5732 14113-14114 -LRB- denotes (
T5731 14109-14112 NN denotes TBI
T5730 14106-14108 NN denotes WT
T5728 14097-14099 IN denotes in
T5727 14092-14096 IN denotes than
T5726 14088-14091 NN denotes TBI
T5725 14085-14087 NN denotes KO
T5724 14079-14084 NN denotes group
T5723 14076-14078 IN denotes in
T5722 14069-14075 JJ denotes higher
T5721 14064-14068 RB denotes also
T5720 14060-14063 VB denotes was
T5719 14057-14059 PRP denotes it
T5718 14053-14056 CC denotes And
T5717 14039-14051 NN denotes counterparts
T5716 14034-14038 NN denotes sham
T5715 14028-14033 PRP-DOLLAR- denotes their
T5714 14023-14027 IN denotes with
T5713 14014-14022 VB denotes compared
T5712 14009-14013 WRB denotes when
T5711 13998-14008 NN denotes astrocytes
T5710 13995-13997 NN denotes KO
T5709 13991-13994 CC denotes and
T5708 13988-13990 NN denotes WT
T5707 13985-13987 IN denotes in
T5706 13978-13984 NN denotes injury
T5705 13970-13977 NN denotes scratch
T5704 13964-13969 IN denotes after
T5703 13952-13963 VB denotes upregulated
T5702 13948-13951 VB denotes was
T5701 13942-13947 NN denotes TNF-α
T5700 13939-13941 IN denotes of
T5699 13933-13938 NN denotes level
T5698 13925-13932 NN denotes protein
T5697 13921-13924 DT denotes the
T5696 13916-13920 IN denotes that
T5695 13907-13915 VB denotes revealed
T5694 13901-13906 NN denotes ELISA
T5693 13898-13900 IN denotes of
T5692 13890-13897 NN denotes results
T5691 13886-13889 DT denotes The
T5690 13883-13884 -RRB- denotes )
T5689 13882-13883 -RRB- denotes )
T5688 13881-13882 NN denotes d
T5687 13880-13881 -LRB- denotes (
T5686 13878-13880 CD denotes –3
T5685 13877-13878 -RRB- denotes )
T5684 13876-13877 DT denotes a
T5683 13875-13876 -LRB- denotes (
T5682 13874-13875 CD denotes 3
T5681 13866-13873 NN denotes Figures
T5680 13865-13866 -LRB- denotes (
T5679 13863-13864 -RRB- denotes )
T5678 13859-13863 CD denotes 0.01
T5677 13857-13858 SYM denotes <
T5676 13855-13856 NN denotes P
T5675 13849-13853 NN denotes IL-6
T5674 13845-13848 IN denotes for
T5673 13839-13844 CD denotes 0.073
T5672 13837-13838 SYM denotes ±
T5671 13832-13836 CD denotes 0.99
T5670 13825-13831 IN denotes versus
T5669 13819-13824 CD denotes 0.102
T5668 13817-13818 SYM denotes ±
T5667 13812-13816 CD denotes 1.28
T5666 13808-13811 CC denotes and
T5665 13806-13807 -COMMA- denotes ,
T5664 13801-13806 NN denotes IL-1β
T5663 13797-13800 IN denotes for
T5662 13791-13796 CD denotes 0.088
T5661 13789-13790 SYM denotes ±
T5660 13784-13788 CD denotes 0.73
T5659 13777-13783 IN denotes versus
T5658 13771-13776 CD denotes 0.064
T5657 13769-13770 SYM denotes ±
T5656 13764-13768 CD denotes 1.34
T5655 13762-13763 -COMMA- denotes ,
T5654 13757-13762 NN denotes TNF-α
T5653 13753-13756 IN denotes for
T5652 13747-13752 CD denotes 0.110
T5651 13745-13746 SYM denotes ±
T5650 13740-13744 CD denotes 1.39
T5649 13733-13739 IN denotes versus
T5648 13727-13732 CD denotes 0.085
T5647 13725-13726 SYM denotes ±
T5646 13720-13724 CD denotes 1.66
T5645 13719-13720 -LRB- denotes (
T5644 13715-13718 NN denotes TBI
T5643 13712-13714 NN denotes WT
T5642 13706-13711 NN denotes group
T5641 13703-13705 IN denotes in
T5640 13697-13702 DT denotes those
T5639 13692-13696 IN denotes than
T5638 13685-13691 JJ denotes higher
T5637 13680-13684 RB denotes much
T5636 13675-13679 VB denotes were
T5635 13671-13674 NNP denotes TBI
T5634 13668-13670 NN denotes KO
T5633 13662-13667 NN denotes group
T5632 13659-13661 IN denotes in
T5631 13649-13658 NN denotes cytokines
T5630 13643-13648 DT denotes these
T5629 13640-13642 IN denotes of
T5628 13633-13639 NN denotes levels
T5627 13628-13632 NN denotes mRNA
T5626 13624-13627 DT denotes the
T5625 13622-13623 -COMMA- denotes ,
T5624 13614-13622 RB denotes Moreover
T5623 13600-13612 RB denotes respectively
T5622 13598-13599 -COMMA- denotes ,
T5621 13586-13598 NN denotes counterparts
T5620 13581-13585 NN denotes sham
T5619 13575-13580 PRP-DOLLAR- denotes their
T5618 13570-13574 IN denotes with
T5617 13561-13569 VB denotes compared
T5616 13556-13560 WRB denotes when
T5615 13545-13555 NN denotes astrocytes
T5614 13542-13544 NN denotes KO
T5613 13538-13541 CC denotes and
T5612 13535-13537 NN denotes WT
T5611 13530-13534 CC denotes both
T5610 13527-13529 IN denotes in
T5609 13519-13526 NN denotes scratch
T5608 13513-13518 IN denotes after
T5607 13503-13512 VB denotes increased
T5606 13498-13502 VB denotes were
T5605 13493-13497 NN denotes IL-6
T5604 13489-13492 CC denotes and
T5603 13487-13488 -COMMA- denotes ,
T5602 13482-13487 NN denotes IL-1β
T5601 13480-13481 -COMMA- denotes ,
T5600 13475-13480 NN denotes TNF-α
T5599 13472-13474 IN denotes of
T5598 13465-13471 NN denotes levels
T5597 13460-13464 NN denotes mRNA
T5596 13456-13459 DT denotes the
T5595 13451-13455 IN denotes that
T5594 13445-13450 VB denotes shown
T5593 13441-13444 VB denotes was
T5592 13438-13440 PRP denotes It
T5591 13431-13436 NN denotes ELISA
T5590 13427-13430 CC denotes and
T5589 13420-13426 NN denotes RT-PCR
T5588 13417-13419 IN denotes by
T5587 13410-13416 NN denotes injury
T5586 13402-13409 NN denotes scratch
T5585 13396-13401 IN denotes after
T5584 13394-13395 NN denotes h
T5583 13391-13393 CD denotes 24
T5582 13388-13390 IN denotes at
T5581 13377-13387 NN denotes astrocytes
T5580 13368-13376 VB denotes cultured
T5579 13365-13367 IN denotes in
T5578 13360-13364 NN denotes IL-6
T5577 13356-13359 CC denotes and
T5576 13354-13355 -COMMA- denotes ,
T5575 13349-13354 NN denotes IL-1β
T5574 13347-13348 -COMMA- denotes ,
T5573 13342-13347 NN denotes TNF-α
T5572 13339-13341 IN denotes of
T5571 13332-13338 NN denotes levels
T5570 13328-13331 DT denotes the
T5569 13321-13327 VB denotes tested
T5568 13318-13320 PRP denotes we
T5567 13316-13317 -COMMA- denotes ,
T5566 13312-13316 RB denotes Here
T5565 13307-13310 NN denotes TBI
T5564 13301-13306 IN denotes after
T5563 13290-13300 NN denotes astrocytes
T5562 13287-13289 IN denotes in
T5561 13282-13286 NN denotes Nrf2
T5560 13278-13281 CC denotes and
T5559 13268-13277 NN denotes cytokines
T5558 13262-13267 DT denotes these
T5557 13254-13261 IN denotes between
T5556 13241-13253 NN denotes relationship
T5555 13237-13240 DT denotes the
T5554 13231-13236 IN denotes about
T5553 13221-13230 JJ denotes ambiguous
T5552 13215-13220 RB denotes still
T5551 13212-13214 VB denotes is
T5550 13209-13211 PRP denotes it
T5549 13205-13208 CC denotes But
T5548 13202-13203 -RRB- denotes ]
T5547 13201-13202 CD denotes 5
T5546 13200-13201 -LRB- denotes [
T5545 13196-13199 NNP denotes TBI
T5544 13190-13195 IN denotes after
T5543 13185-13189 NN denotes mice
T5542 13170-13184 JJ denotes Nrf2-deficient
T5541 13167-13169 IN denotes of
T5540 13161-13166 NN denotes brain
T5539 13157-13160 DT denotes the
T5538 13154-13156 IN denotes in
T5537 13145-13153 VB denotes observed
T5536 13140-13144 VB denotes were
T5535 13130-13139 NN denotes cytokines
T5534 13114-13129 JJ denotes proinflammatory
T5533 13109-13113 JJ denotes such
T5532 13106-13108 IN denotes of
T5531 13099-13105 NN denotes levels
T5530 13092-13098 JJ denotes higher
T5529 13087-13091 IN denotes that
T5528 13080-13086 VB denotes proved
T5527 13076-13079 VB denotes has
T5526 13070-13075 NN denotes study
T5525 13061-13069 JJ denotes previous
T5524 13057-13060 PRP-DOLLAR- denotes our
T5523 13053-13056 CC denotes And
T5522 13050-13051 -RRB- denotes ]
T5521 13049-13050 CD denotes 8
T5520 13047-13048 -COMMA- denotes ,
T5519 13046-13047 CD denotes 2
T5518 13045-13046 -LRB- denotes [
T5517 13037-13044 NN denotes animals
T5516 13034-13036 IN denotes in
T5515 13030-13033 NN denotes TBI
T5514 13024-13029 IN denotes after
T5513 13014-13023 VB denotes increased
T5493 12883-12886 VB denotes are
T5492 12878-12882 NN denotes IL-6
T5491 12874-12877 CC denotes and
T5490 12872-12873 -COMMA- denotes ,
T5489 12867-12872 NN denotes IL-1β
T5488 12865-12866 -COMMA- denotes ,
T5487 12860-12865 NN denotes TNF-α
T5486 12858-12859 -COMMA- denotes ,
T5485 12854-12858 VB denotes know
T5484 12850-12853 DT denotes all
T5483 12847-12849 PRP denotes we
T5482 12844-12846 IN denotes As
T5481 12837-12843 NNP denotes Injury
T5480 12829-12836 NNP denotes Scratch
T5479 12823-12828 IN denotes after
T5478 12812-12822 NN denotes Astrocytes
T5477 12803-12811 NNP denotes Knockout
T5476 12798-12802 NN denotes Nrf2
T5475 12795-12797 IN denotes in
T5474 12786-12794 VB denotes Elevated
T5473 12782-12785 VB denotes Was
T5472 12772-12781 NN denotes Cytokines
T5471 12756-12771 JJ denotes Proinflammatory
T5470 12753-12755 IN denotes of
T5469 12742-12752 NN denotes Expression
T5468 12737-12740 CD denotes 3.3
T5729 14100-14105 NN denotes group
T5207 12725-12731 NN denotes Figure
T5206 12724-12725 -LRB- denotes (
T5205 12722-12723 -RRB- denotes )
T5204 12718-12722 CD denotes 0.01
T5203 12716-12717 SYM denotes <
T5202 12714-12715 NN denotes P
T5201 12712-12713 -COMMA- denotes ,
T5200 12707-12712 CD denotes 0.072
T5199 12705-12706 SYM denotes ±
T5198 12700-12704 CD denotes 2.28
T5197 12693-12699 IN denotes versus
T5196 12687-12692 CD denotes 0.173
T5195 12685-12686 SYM denotes ±
T5194 12680-12684 CD denotes 2.67
T5193 12679-12680 -LRB- denotes (
T5192 12675-12678 NN denotes TBI
T5191 12672-12674 NN denotes WT
T5190 12666-12671 NN denotes group
T5189 12663-12665 IN denotes in
T5188 12658-12662 IN denotes than
T5187 12654-12657 NN denotes TBI
T5186 12651-12653 NN denotes KO
T5185 12645-12650 NN denotes group
T5184 12642-12644 IN denotes in
T5183 12633-12641 VB denotes observed
T5182 12629-12632 VB denotes was
T5181 12620-12628 NN denotes activity
T5180 12614-12619 NN denotes NF-κB
T5179 12607-12613 JJ denotes higher
T5178 12601-12606 IN denotes while
T5177 12599-12600 -COMMA- denotes ,
T5176 12590-12599 NN denotes genotypes
T5175 12585-12589 DT denotes both
T5174 12582-12584 IN denotes of
T5173 12571-12581 NN denotes astrocytes
T5172 12568-12570 IN denotes in
T5171 12562-12567 NN denotes NF-κB
T5170 12559-12561 IN denotes of
T5169 12548-12558 NN denotes activation
T5168 12540-12547 VB denotes induced
T5167 12533-12539 NN denotes injury
T5166 12525-12532 NN denotes Scratch
T5165 12517-12523 NN denotes injury
T5164 12509-12516 NN denotes scratch
T5163 12503-12508 IN denotes after
T5162 12501-12502 NN denotes h
T5161 12498-12500 CD denotes 24
T5160 12495-12497 IN denotes at
T5159 12490-12494 NN denotes EMSA
T5158 12487-12489 IN denotes by
T5157 12482-12486 NN denotes mice
T5156 12479-12481 NN denotes KO
T5155 12476-12478 CC denotes or
T5154 12473-12475 NN denotes WT
T5153 12468-12472 NN denotes Nrf2
T5152 12463-12467 IN denotes from
T5151 12452-12462 NN denotes astrocytes
T5150 12449-12451 IN denotes of
T5149 12443-12448 NN denotes NF-κB
T5148 12440-12442 IN denotes of
T5147 12431-12439 NN denotes activity
T5146 12419-12430 JJ denotes DNA-binding
T5145 12415-12418 DT denotes the
T5144 12407-12414 VB denotes studied
T5143 12404-12406 PRP denotes we
T5142 12402-12403 -COMMA- denotes ,
T5141 12398-12402 RB denotes Here
T5140 12395-12396 -RRB- denotes ]
T5139 12394-12395 CD denotes 2
T5138 12393-12394 -LRB- denotes [
T5137 12389-12392 NNP denotes TBI
T5136 12383-12388 IN denotes after
T5135 12377-12382 NN denotes brain
T5134 12374-12376 IN denotes in
T5133 12364-12373 VB denotes activated
T5132 12361-12363 VB denotes is
T5131 12355-12360 NN denotes NF-κB
T5130 12350-12354 IN denotes that
T5129 12341-12349 VB denotes reported
T5128 12336-12340 VB denotes been
T5127 12332-12335 VB denotes has
T5126 12329-12331 PRP denotes It
T5125 12322-12328 NNP denotes Injury
T5124 12314-12321 NNP denotes Scratch
T5123 12308-12313 IN denotes after
T5122 12299-12307 NN denotes Activity
T5121 12287-12298 JJ denotes DNA-Binding
T5120 12281-12286 NN denotes NF-κB
T5119 12278-12280 IN denotes of
T5118 12265-12277 NN denotes Upregulation
T5117 12256-12264 VB denotes Enhanced
T5116 12251-12255 NN denotes Nrf2
T5115 12248-12250 IN denotes of
T5114 12237-12247 NN denotes Disruption
T5113 12232-12235 CD denotes 3.2
T5512 13010-13013 VB denotes are
T5511 13000-13009 NN denotes cytokines
T5510 12984-12999 JJ denotes proinflammatory
T5509 12978-12983 DT denotes these
T5508 12973-12977 IN denotes that
T5507 12964-12972 VB denotes revealed
T5506 12959-12963 VB denotes been
T5505 12955-12958 VB denotes has
T5504 12952-12954 PRP denotes It
T5503 12945-12950 NN denotes NF-κB
T5502 12942-12944 IN denotes of
T5501 12933-12941 NN denotes activity
T5500 12922-12932 JJ denotes endogenous
T5499 12918-12921 DT denotes the
T5498 12910-12917 VB denotes reflect
T5497 12906-12909 CC denotes and
T5496 12900-12905 NN denotes NF-κB
T5495 12897-12899 IN denotes by
T5494 12887-12896 VB denotes regulated
T4942 12108-12113 WDT denotes which
T4941 12106-12107 -COMMA- denotes ,
T4940 12105-12106 -RRB- denotes )
T4939 12101-12105 CD denotes 0.01
T4938 12099-12100 SYM denotes <
T4937 12097-12098 NN denotes P
T4936 12096-12097 -LRB- denotes (
T4935 12094-12095 -RRB- denotes )
T4934 12089-12094 CD denotes 0.098
T4933 12087-12088 CC denotes ±
T4932 12082-12086 CD denotes 0.98
T4931 12081-12082 -LRB- denotes (
T4930 12077-12080 NN denotes TBI
T4929 12074-12076 NN denotes WT
T4928 12068-12073 NN denotes group
T4927 12065-12067 IN denotes of
T4926 12060-12064 DT denotes that
T4925 12055-12059 IN denotes than
T4924 12053-12054 -RRB- denotes )
T4923 12048-12053 CD denotes 0.064
T4922 12046-12047 CC denotes ±
T4921 12041-12045 CD denotes 0.38
T4920 12040-12041 -LRB- denotes (
T4919 12036-12039 NN denotes TBI
T4918 12033-12035 NN denotes KO
T4917 12027-12032 NN denotes group
T4916 12024-12026 IN denotes of
T4915 12018-12023 NN denotes value
T4914 12015-12017 NN denotes OD
T4913 12009-12014 JJ denotes lower
T4912 12002-12008 VB denotes showed
T4911 11997-12001 RB denotes also
T4910 11991-11996 NN denotes assay
T4909 11985-11990 NN denotes CCK-8
T4908 11980-11983 NNP denotes TBI
T4907 11977-11979 NNP denotes KO
T4906 11971-11976 NN denotes group
T4905 11968-11970 IN denotes in
T4904 11963-11967 NN denotes line
T4903 11955-11962 NN denotes scratch
T4902 11952-11954 IN denotes of
T4901 11946-11951 NN denotes sides
T4900 11941-11945 DT denotes both
T4899 11938-11940 IN denotes in
T4898 11929-11937 VB denotes observed
T4897 11922-11928 RB denotes mostly
T4896 11917-11921 VB denotes were
T4895 11906-11916 NN denotes distortion
T4894 11898-11905 NN denotes process
T4893 11886-11897 JJ denotes cytoplasmic
T4892 11882-11885 CC denotes and
T4891 11880-11881 -COMMA- denotes ,
T4890 11876-11880 NN denotes lost
T4889 11871-11875 NN denotes cell
T4888 11869-11870 -COMMA- denotes ,
T4887 11864-11869 NN denotes plate
T4886 11856-11863 NN denotes culture
T4885 11851-11855 IN denotes from
T4884 11840-11850 NN denotes detachment
T4883 11836-11839 DT denotes The
T4882 11827-11834 NN denotes scratch
T4881 11821-11826 IN denotes after
T4880 11819-11820 NN denotes h
T4879 11816-11818 CD denotes 24
T4878 11813-11815 IN denotes at
T4877 11804-11812 NN denotes analysis
T4876 11798-11803 NN denotes CCK-8
T4875 11794-11797 CC denotes and
T4874 11783-11793 NN denotes microscope
T4873 11780-11782 IN denotes by
T4872 11771-11779 VB denotes detected
T4871 11767-11770 VB denotes was
T4870 11761-11766 NN denotes death
T4869 11756-11760 NN denotes Cell
T4868 11749-11755 NNP denotes Injury
T4867 11741-11748 NNP denotes Scratch
T4866 11735-11740 IN denotes after
T4865 11724-11734 NN denotes Astrocytes
T4864 11721-11723 IN denotes in
T4863 11715-11720 NNP denotes Death
T4862 11710-11714 NNP denotes Cell
T4861 11706-11709 DT denotes the
T4860 11695-11705 VB denotes Aggravated
T4859 11690-11694 NN denotes Nrf2
T4858 11687-11689 IN denotes of
T4857 11677-11686 NN denotes Depletion
T4856 11672-11675 CD denotes 3.1
T4798 11649-11657 NN denotes software
T4797 11644-11648 CD denotes 18.0
T4796 11639-11643 NN denotes SPSS
T4795 11633-11638 VB denotes using
T4794 11630-11632 IN denotes by
T4793 11620-11629 VB denotes performed
T4792 11615-11619 VB denotes were
T4791 11606-11614 NN denotes analyses
T4790 11602-11605 DT denotes All
T4789 11589-11600 JJ denotes significant
T4788 11586-11588 VB denotes be
T4787 11583-11585 TO denotes to
T4786 11572-11582 VB denotes considered
T4785 11567-11571 VB denotes were
T4784 11562-11566 CD denotes 0.05
T4783 11561-11562 SYM denotes <
T4782 11554-11560 NN denotes values
T4781 11552-11553 NN denotes P
T4780 11546-11550 NN denotes test
T4779 11535-11545 NN denotes comparison
T4778 11526-11534 JJ denotes multiple
T4777 11522-11525 NN denotes LSD
T4776 11518-11521 CC denotes and
T4775 11512-11517 NN denotes ANOVA
T4774 11509-11511 IN denotes by
T4773 11499-11508 VB denotes evaluated
T4772 11495-11498 CC denotes and
T4771 11492-11494 NN denotes SD
T4770 11490-11491 SYM denotes ±
T4769 11485-11489 NN denotes mean
T4768 11482-11484 IN denotes as
T4767 11472-11481 VB denotes expressed
T4766 11467-11471 VB denotes were
T4765 11462-11466 NN denotes Data
T4764 11453-11461 NN denotes Analysis
T4763 11441-11452 JJ denotes Statistical
T4762 11436-11439 CD denotes 2.9
T5209 12733-12734 -RRB- denotes )
T5208 12732-12733 CD denotes 2
T4496 11116-11118 TO denotes to
T4495 11111-11115 NN denotes MMP9
T4494 11107-11110 DT denotes the
T4493 11100-11106 VB denotes enable
T4492 11097-11099 TO denotes to
T4491 11092-11096 NN denotes 37°C
T4490 11089-11091 IN denotes at
T4489 11087-11088 NN denotes h
T4488 11084-11086 CD denotes 40
T4487 11080-11083 IN denotes for
T4486 11073-11079 NN denotes buffer
T4485 11063-11072 NN denotes substrate
T4484 11060-11062 IN denotes in
T4483 11050-11059 VB denotes incubated
T4482 11045-11049 RB denotes then
T4481 11041-11044 CC denotes and
T4480 11035-11040 NN denotes X-100
T4479 11028-11034 NN denotes Triton
T4478 11026-11027 NN denotes %
T4477 11023-11026 CD denotes 2.5
T4476 11020-11022 IN denotes in
T4475 11009-11019 NN denotes incubation
T4474 11006-11008 IN denotes by
T4473 10996-11005 VB denotes renatured
T4472 10991-10995 VB denotes were
T4471 10982-10990 NN denotes proteins
T4470 10978-10981 DT denotes the
T4469 10973-10977 RB denotes Then
T4468 10956-10971 NN denotes electrophoresis
T4467 10953-10955 IN denotes by
T4466 10943-10952 VB denotes separated
T4465 10938-10942 VB denotes were
T4464 10929-10937 NN denotes extracts
T4463 10921-10928 NN denotes protein
T4462 10911-10920 JJ denotes cytosolic
T4461 10908-10910 NN denotes μg
T4460 10905-10907 CD denotes 40
T4459 10903-10904 -COMMA- denotes ,
T4458 10896-10903 RB denotes Briefly
T4457 10893-10894 -RRB- denotes )
T4456 10890-10893 NNP denotes USA
T4455 10888-10889 -COMMA- denotes ,
T4454 10886-10888 NNP denotes MA
T4453 10884-10885 -COMMA- denotes ,
T4452 10881-10884 NNP denotes Inc
T4451 10869-10880 NNP denotes Scientifics
T4450 10862-10868 NNP denotes Genmed
T4449 10861-10862 -LRB- denotes (
T4448 10848-10860 NN denotes instructions
T4447 10845-10847 POS denotes 's
T4446 10833-10845 NN denotes manufacturer
T4445 10829-10832 DT denotes the
T4444 10826-10828 TO denotes to
T4443 10816-10825 VB denotes according
T4442 10806-10815 VB denotes performed
T4441 10802-10805 VB denotes was
T4440 10791-10801 NN denotes zymography
T4439 10783-10790 NN denotes Gelatin
T4438 10776-10781 NN denotes –20°C
T4437 10773-10775 IN denotes at
T4436 10766-10772 VB denotes stored
T4435 10762-10765 CC denotes and
T4434 10747-10761 NN denotes centrifugation
T4433 10744-10746 IN denotes by
T4432 10734-10743 VB denotes separated
T4431 10729-10733 VB denotes were
T4430 10720-10728 NN denotes extracts
T4429 10712-10719 NNP denotes Soluble
T4428 10704-10711 NNP denotes Triton.
T4427 10702-10703 NN denotes %
T4426 10698-10702 CD denotes 0.01
T4425 10694-10697 CC denotes and
T4424 10692-10693 -COMMA- denotes ,
T4423 10688-10692 NN denotes NaN3
T4422 10685-10687 NN denotes mM
T4421 10681-10684 CD denotes 0.2
T4420 10679-10680 -COMMA- denotes ,
T4419 10674-10679 NN denotes CaCl2
T4418 10671-10673 NN denotes mM
T4417 10669-10670 CD denotes 5
T4416 10667-10668 -COMMA- denotes ,
T4415 10663-10667 NN denotes NaCl
T4414 10660-10662 NN denotes mM
T4413 10656-10659 CD denotes 150
T4412 10654-10655 -COMMA- denotes ,
T4411 10653-10654 -RRB- denotes )
T4410 10650-10653 CD denotes 7.4
T4409 10647-10649 NN denotes pH
T4408 10646-10647 -LRB- denotes (
T4407 10637-10645 NN denotes Tris-HCl
T4406 10634-10636 NN denotes mM
T4405 10631-10633 CD denotes 50
T4404 10620-10630 VB denotes containing
T4403 10613-10619 NN denotes buffer
T4402 10607-10612 NN denotes lysis
T4401 10604-10606 IN denotes in
T4400 10592-10603 VB denotes homogenized
T4399 10587-10591 VB denotes were
T4398 10580-10586 NN denotes groups
T4397 10575-10579 CD denotes four
T4396 10572-10574 IN denotes of
T4395 10566-10571 NN denotes Cells
T4394 10555-10565 NN denotes Zymography
T4393 10546-10554 NN denotes Gelatine
T4392 10541-10544 CD denotes 2.8
T4963 12223-12229 NN denotes groups
T4962 12209-12222 JJ denotes sham-operated
T4961 12205-12208 CD denotes two
T4960 12197-12204 IN denotes between
T4959 12186-12196 NN denotes difference
T4958 12183-12185 DT denotes no
T4957 12179-12182 VB denotes was
T4956 12173-12178 EX denotes There
T4955 12170-12171 -RRB- denotes )
T4954 12169-12170 CD denotes 1
T4953 12162-12168 NN denotes Figure
T4952 12161-12162 -LRB- denotes (
T4951 12157-12160 NN denotes TBI
T4950 12154-12156 NN denotes KO
T4949 12148-12153 NN denotes group
T4948 12145-12147 IN denotes in
T4947 12139-12144 NN denotes death
T4946 12134-12138 NN denotes cell
T4945 12129-12133 JJ denotes more
T4944 12119-12128 VB denotes suggested
T4943 12114-12118 RB denotes also
T4281 10531-10538 NN denotes protein
T4184 9976-9985 VB denotes microwell
T4183 9973-9975 TO denotes to
T4182 9967-9972 VB denotes added
T4181 9962-9966 VB denotes were
T4180 9946-9961 NN denotes biotinconjugate
T4179 9942-9945 CC denotes and
T4178 9934-9941 NN denotes samples
T4177 9929-9933 RB denotes Then
T4176 9922-9927 NN denotes curve
T4175 9913-9921 JJ denotes standard
T4174 9904-9912 VB denotes building
T4173 9900-9903 IN denotes for
T4172 9891-9899 NN denotes dilution
T4171 9882-9890 JJ denotes standard
T4170 9878-9881 DT denotes the
T4169 9870-9877 VB denotes created
T4168 9866-9869 CC denotes and
T4167 9857-9865 NN denotes standard
T4166 9853-9856 DT denotes the
T4165 9844-9852 VB denotes prepared
T4164 9842-9843 -COMMA- denotes ,
T4163 9835-9842 NNP denotes Briefly
T4162 9832-9833 -RRB- denotes )
T4161 9829-9832 NNP denotes USA
T4160 9827-9828 -COMMA- denotes ,
T4159 9825-9827 NNP denotes CA
T4158 9820-9824 NNP denotes Inc.
T4157 9809-9819 NNP denotes MedSystems
T4156 9802-9808 NNP denotes Bender
T4155 9801-9802 -LRB- denotes (
T4154 9788-9800 NN denotes instructions
T4153 9785-9787 POS denotes 's
T4152 9773-9785 NN denotes manufacturer
T4151 9769-9772 DT denotes the
T4150 9766-9768 TO denotes to
T4149 9756-9765 VB denotes according
T4148 9750-9755 NN denotes mouse
T4147 9746-9749 IN denotes for
T4146 9737-9745 JJ denotes specific
T4145 9732-9736 NN denotes kits
T4144 9726-9731 NN denotes ELISA
T4143 9720-9725 VB denotes using
T4142 9709-9719 VB denotes quantified
T4141 9704-9708 VB denotes were
T4140 9696-9703 NN denotes protein
T4139 9694-9695 -RRB- denotes )
T4138 9690-9694 NN denotes IL-6
T4137 9689-9690 -LRB- denotes (
T4136 9675-9688 NN denotes interleukin-6
T4135 9671-9674 CC denotes and
T4134 9669-9670 -COMMA- denotes ,
T4133 9668-9669 -RRB- denotes )
T4132 9663-9668 NN denotes IL-1β
T4131 9662-9663 -LRB- denotes (
T4130 9657-9661 NN denotes beta
T4129 9643-9656 NN denotes interleukin-1
T4128 9641-9642 -COMMA- denotes ,
T4127 9640-9641 -RRB- denotes )
T4126 9635-9640 NN denotes TNF-α
T4125 9634-9635 -LRB- denotes (
T4124 9621-9633 NN denotes factor-alpha
T4123 9612-9620 NN denotes necrosis
T4122 9606-9611 NN denotes tumor
T4121 9603-9605 IN denotes of
T4120 9596-9602 NN denotes Levels
T4119 9593-9594 -RRB- denotes )
T4118 9590-9593 NNP denotes USA
T4117 9588-9589 -COMMA- denotes ,
T4116 9586-9588 NNP denotes IL
T4115 9584-9585 -COMMA- denotes ,
T4114 9578-9584 NNP denotes Pierce
T4113 9577-9578 -LRB- denotes (
T4112 9569-9576 NNP denotes Reagent
T4111 9563-9568 NNP denotes Assay
T4110 9555-9562 NNP denotes Protein
T4109 9550-9554 NNP denotes Plus
T4108 9540-9549 NNP denotes Coomassie
T4107 9537-9539 IN denotes by
T4106 9526-9536 VB denotes determined
T4105 9522-9525 VB denotes was
T4104 9514-9521 NN denotes protein
T4103 9508-9513 JJ denotes total
T4102 9504-9507 CC denotes and
T4101 9502-9503 -COMMA- denotes ,
T4549 11427-11433 NN denotes ImageJ
T4548 11419-11426 NN denotes program
T4547 11410-11418 NN denotes analysis
T4546 11404-11409 NN denotes image
T4545 11399-11403 IN denotes with
T4544 11389-11398 VB denotes performed
T4543 11385-11388 VB denotes was
T4542 11377-11384 NN denotes density
T4541 11372-11376 NN denotes band
T4540 11367-11371 NN denotes MMP9
T4539 11364-11366 IN denotes of
T4538 11349-11363 NN denotes Quantification
T4537 11338-11347 NN denotes substrate
T4536 11334-11337 DT denotes the
T4535 11331-11333 IN denotes of
T4534 11325-11330 NN denotes lysis
T4533 11321-11324 DT denotes the
T4532 11311-11320 VB denotes indicates
T4531 11305-11310 WDT denotes which
T4530 11301-11304 NN denotes gel
T4529 11296-11300 JJ denotes blue
T4528 11293-11295 IN denotes in
T4527 11287-11292 NN denotes bands
T4526 11281-11286 JJ denotes clear
T4525 11278-11280 IN denotes by
T4524 11271-11277 VB denotes showed
T4523 11266-11270 VB denotes were
T4522 11255-11265 NN denotes activities
T4521 11243-11254 JJ denotes Proteolytic
T4520 11233-11241 NN denotes methanol
T4519 11231-11232 NN denotes %
T4518 11229-11231 CD denotes 50
T4517 11226-11228 IN denotes in
T4516 11216-11225 VB denotes destained
T4515 11212-11215 CC denotes and
T4514 11210-11211 NN denotes h
T4513 11208-11209 CD denotes 1
T4512 11204-11207 IN denotes for
T4511 11199-11203 JJ denotes blue
T4510 11189-11198 NNP denotes Coomassie
T4509 11184-11188 IN denotes with
T4508 11176-11183 VB denotes stained
T4507 11172-11175 VB denotes was
T4506 11168-11171 NN denotes gel
T4505 11163-11167 DT denotes each
T4504 11161-11162 -COMMA- denotes ,
T4503 11156-11161 NN denotes water
T4502 11153-11155 IN denotes in
T4501 11145-11152 VB denotes rinsing
T4500 11139-11144 IN denotes After
T4499 11130-11137 NN denotes gelatin
T4498 11126-11129 DT denotes the
T4497 11119-11125 VB denotes cleave
T3671 9357-9365 NN denotes standard
T3670 9348-9356 JJ denotes internal
T3669 9345-9347 IN denotes as
T3668 9340-9344 VB denotes used
T3667 9336-9339 VB denotes was
T3666 9328-9335 NN denotes β-actin
T3665 9325-9327 IN denotes of
T3664 9319-9324 NN denotes level
T3663 9315-9318 DT denotes The
T3662 9308-9313 NN denotes blots
T3661 9304-9307 DT denotes the
T3660 9301-9303 IN denotes of
T3659 9291-9300 NN denotes intensity
T3658 9287-9290 DT denotes the
T3657 9279-9286 VB denotes analyze
T3656 9276-9278 TO denotes to
T3655 9271-9275 VB denotes used
T3654 9267-9270 VB denotes was
T3653 9260-9266 NN denotes ImageJ
T3652 9257-9258 -RRB- denotes )
T3651 9252-9257 NNP denotes Japan
T3650 9250-9251 -COMMA- denotes ,
T3649 9245-9250 NNP denotes Tokyo
T3648 9243-9244 -COMMA- denotes ,
T3647 9234-9243 NNP denotes Hyperfilm
T3646 9229-9233 NNP denotes Fuji
T3645 9228-9229 -LRB- denotes (
T3644 9223-9227 NN denotes film
T3643 9210-9222 JJ denotes radiographic
T3642 9207-9209 TO denotes to
T3641 9199-9206 VB denotes exposed
T3640 9195-9198 CC denotes and
T3639 9193-9194 -RRB- denotes )
T3638 9191-9193 NNP denotes UK
T3637 9189-9190 -COMMA- denotes ,
T3636 9184-9189 NNP denotes Bucks
T3635 9182-9183 -COMMA- denotes ,
T3634 9171-9182 NNP denotes Biosciences
T3633 9162-9170 NNP denotes Amersham
T3632 9161-9162 -LRB- denotes (
T3631 9154-9160 NN denotes system
T3630 9144-9153 NN denotes detection
T3629 9142-9143 -RRB- denotes )
T3628 9139-9142 NN denotes ECL
T3627 9138-9139 -LRB- denotes (
T3626 9120-9137 NN denotes chemiluminescence
T3625 9111-9119 VB denotes enhanced
T3624 9106-9110 IN denotes with
T3623 9096-9105 VB denotes incubated
T3622 9091-9095 VB denotes were
T3621 9085-9090 NN denotes Blots
T3620 9082-9083 NN denotes h
T3619 9080-9081 CD denotes 1
T3618 9076-9079 IN denotes for
T3617 9067-9075 NN denotes dilution
T3616 9062-9066 CD denotes 2000
T3615 9060-9061 -COLON- denotes :
T3614 9058-9059 CD denotes 1
T3613 9056-9057 DT denotes a
T3612 9053-9055 IN denotes at
T3611 9044-9052 NN denotes antibody
T3610 9034-9043 JJ denotes secondary
T3609 9016-9033 JJ denotes peroxidase-linked
T3608 9004-9015 JJ denotes horseradish
T3607 9000-9003 DT denotes the
T3606 8995-8999 IN denotes with
T3605 8987-8994 VB denotes covered
T3604 8983-8986 CC denotes and
T3603 8976-8982 NN denotes buffer
T3602 8968-8975 VB denotes washing
T3601 8964-8967 DT denotes the
T3600 8961-8963 IN denotes in
T3599 8955-8960 NN denotes times
T3598 8949-8954 CD denotes three
T3597 8942-8948 VB denotes washed
T3596 8937-8941 VB denotes were
T3595 8931-8936 NN denotes blots
T3594 8927-8930 DT denotes The
T3593 8914-8925 NN denotes temperature
T3592 8909-8913 NN denotes room
T3591 8906-8908 IN denotes at
T3590 8904-8905 NN denotes h
T3589 8902-8903 CD denotes 1
T3588 8898-8901 IN denotes for
T3587 8892-8897 NN denotes blots
T3586 8888-8891 DT denotes the
T3585 8885-8887 TO denotes to
T3584 8879-8884 VB denotes added
T3583 8874-8878 RB denotes then
T3582 8870-8873 CC denotes and
T3581 8863-8869 NN denotes buffer
T3580 8854-8862 VB denotes blocking
T3579 8851-8853 IN denotes in
T3578 8837-8850 RB denotes appropriately
T3577 8829-8836 VB denotes diluted
T3576 8825-8828 VB denotes was
T3575 8816-8824 NN denotes antibody
T3574 8808-8815 JJ denotes primary
T3573 8803-8807 DT denotes Each
T3572 8800-8801 -RRB- denotes )
T3571 8797-8800 NN denotes kDa
T3570 8794-8796 CD denotes 92
T3569 8792-8793 -COMMA- denotes ,
T3568 8789-8792 NNP denotes USA
T3567 8787-8788 -COMMA- denotes ,
T3566 8785-8787 NNP denotes CA
T3565 8783-8784 -COMMA- denotes ,
T3564 8770-8783 NNP denotes Biotechnology
T3563 8765-8769 NNP denotes Cruz
T3562 8759-8764 NNP denotes Santa
T3561 8757-8758 -COMMA- denotes ,
T3560 8750-8757 NN denotes sc-6841
T3559 8749-8750 -LRB- denotes (
T3558 8747-8748 -RRB- denotes )
T3557 8743-8747 NN denotes MMP9
T3556 8742-8743 -LRB- denotes (
T3555 8740-8741 CD denotes 9
T3554 8723-8739 NN denotes metallopeptidase
T3553 8711-8722 JJ denotes anti-matrix
T3552 8707-8710 CC denotes and
T3551 8705-8706 -RRB- denotes )
T3550 8702-8705 NN denotes kDa
T3549 8699-8701 CD denotes 43
T3548 8697-8698 -COMMA- denotes ,
T3547 8694-8697 NNP denotes USA
T3546 8692-8693 -COMMA- denotes ,
T3545 8690-8692 NNP denotes CA
T3544 8688-8689 -COMMA- denotes ,
T3543 8675-8688 NNP denotes Biotechnology
T3542 8670-8674 NNP denotes Cruz
T3541 8664-8669 NNP denotes Santa
T3540 8662-8663 -COMMA- denotes ,
T3539 8653-8662 NN denotes sc-130657
T3538 8652-8653 -LRB- denotes (
T3537 8639-8651 JJ denotes anti-β-actin
T3536 8637-8638 -COLON- denotes :
T3535 8633-8637 VB denotes used
T3534 8628-8632 VB denotes were
T3533 8617-8627 NN denotes antibodies
T3532 8607-8616 VB denotes following
T3531 8603-8606 DT denotes the
T3530 8599-8602 CC denotes and
T3529 8597-8598 -COMMA- denotes ,
T3528 8594-8597 NN denotes 4°C
T3527 8591-8593 IN denotes at
T3526 8581-8590 RB denotes overnight
T3525 8574-8580 NN denotes buffer
T3524 8567-8573 NN denotes saline
T3523 8564-8566 IN denotes in
T3522 8559-8563 NN denotes milk
T3521 8555-8558 JJ denotes dry
T3520 8548-8554 JJ denotes nonfat
T3519 8546-8547 NN denotes %
T3518 8545-8546 CD denotes 5
T3517 8540-8544 IN denotes with
T3516 8532-8539 VB denotes blocked
T3515 8527-8531 VB denotes were
T4280 10525-10530 JJ denotes total
T4279 10522-10524 IN denotes of
T4278 10516-10521 NN denotes pg/mg
T4277 10513-10515 IN denotes as
T4276 10503-10512 VB denotes expressed
T4275 10499-10502 CC denotes and
T4274 10493-10498 NN denotes curve
T4273 10484-10492 JJ denotes standard
T4272 10480-10483 DT denotes the
T4271 10477-10479 TO denotes to
T4270 10467-10476 VB denotes according
T4269 10456-10466 VB denotes determined
T4268 10452-10455 VB denotes was
T4267 10444-10451 NN denotes protein
T4266 10441-10443 IN denotes of
T4265 10427-10440 NN denotes concentration
T4264 10423-10426 DT denotes The
T4263 10420-10421 -RRB- denotes )
T4262 10417-10420 NNP denotes USA
T4261 10415-10416 -COMMA- denotes ,
T4260 10413-10415 NNP denotes CA
T4259 10411-10412 -COMMA- denotes ,
T4258 10399-10411 NNP denotes Laboratories
T4257 10391-10398 NNP denotes Bio-Rad
T4256 10390-10391 -LRB- denotes (
T4255 10383-10389 NN denotes reader
T4254 10372-10382 NN denotes microplate
T4253 10366-10371 NN denotes ELISA
T4252 10358-10365 JJ denotes Bio-Rad
T4251 10356-10357 DT denotes a
T4250 10350-10355 VB denotes using
T4249 10347-10349 NN denotes nm
T4248 10343-10346 CD denotes 450
T4247 10340-10342 IN denotes at
T4246 10331-10339 VB denotes measured
T4245 10327-10330 VB denotes was
T4244 10317-10326 NN denotes intensity
T4243 10310-10316 NN denotes colour
T4242 10306-10309 DT denotes The
T4241 10299-10304 VB denotes added
T4240 10295-10298 VB denotes was
T4239 10286-10294 NN denotes solution
T4238 10281-10285 NN denotes stop
T4237 10277-10280 DT denotes the
T4236 10275-10276 -COMMA- denotes ,
T4235 10272-10275 NN denotes min
T4234 10266-10271 CD denotes 10–30
T4233 10260-10265 RB denotes about
T4232 10256-10259 IN denotes for
T4231 10246-10255 VB denotes incubated
T4230 10240-10245 IN denotes After
T4229 10229-10238 NN denotes substrate
T4228 10225-10228 NN denotes TMB
T4227 10218-10224 VB denotes adding
T4226 10215-10217 IN denotes by
T4225 10206-10214 VB denotes followed
T4224 10200-10205 NN denotes times
T4223 10198-10199 CD denotes 3
T4222 10191-10197 VB denotes washed
T4221 10186-10190 VB denotes were
T4220 10179-10185 NN denotes strips
T4219 10169-10178 NN denotes microwell
T4218 10167-10168 -COMMA- denotes ,
T4217 10166-10167 NN denotes h
T4216 10164-10165 CD denotes 1
T4215 10160-10163 IN denotes for
T4214 10150-10159 VB denotes incubated
T4213 10144-10149 IN denotes After
T4212 10137-10142 NN denotes wells
T4211 10133-10136 DT denotes all
T4210 10130-10132 TO denotes to
T4209 10124-10129 VB denotes added
T4208 10119-10123 VB denotes were
T4207 10102-10118 NN denotes streptavidin-HRP
T4206 10098-10101 CC denotes and
T4205 10096-10097 -COMMA- denotes ,
T4204 10090-10096 NN denotes buffer
T4203 10085-10089 NN denotes wash
T4202 10080-10084 IN denotes with
T4201 10074-10079 NN denotes times
T4200 10072-10073 CD denotes 3
T4199 10065-10071 VB denotes washed
T4198 10060-10064 VB denotes were
T4197 10053-10059 NN denotes strips
T4196 10043-10052 NN denotes microwell
T4195 10039-10042 DT denotes the
T4194 10037-10038 -COMMA- denotes ,
T4193 10026-10037 NN denotes temperature
T4192 10021-10025 NN denotes room
T4191 10018-10020 IN denotes at
T4190 10016-10017 NN denotes h
T4189 10014-10015 CD denotes 2
T4188 10010-10013 IN denotes for
T4187 10000-10009 VB denotes incubated
T4186 9994-9999 IN denotes After
T4185 9986-9992 NN denotes strips
T3514 8517-8526 NN denotes membranes
T3513 8515-8516 -COMMA- denotes ,
T3512 8501-8515 NN denotes immunoblotting
T3511 8497-8500 IN denotes For
T3510 8487-8495 NN denotes membrane
T3509 8472-8486 NN denotes nitrocellulose
T3508 8470-8471 DT denotes a
T3507 8465-8469 IN denotes onto
T3506 8450-8464 VB denotes electroblotted
T3505 8446-8449 CC denotes and
T3504 8444-8445 -COMMA- denotes ,
T3503 8429-8444 NN denotes electrophoresis
T3502 8425-8428 NN denotes gel
T3501 8410-8424 NN denotes polyacrylamide
T3500 8402-8409 NN denotes sulfate
T3499 8394-8401 NN denotes dodecyl
T3498 8387-8393 NN denotes sodium
T3497 8385-8386 NN denotes %
T3496 8383-8385 CD denotes 10
T3495 8380-8382 IN denotes by
T3494 8370-8379 VB denotes separated
T3493 8368-8369 -COMMA- denotes ,
T3492 8362-8368 NN denotes buffer
T3491 8354-8361 NN denotes loading
T3490 8347-8353 NN denotes sample
T3489 8339-8346 NNP denotes Laemmli
T3221 7849-7857 NN denotes standard
T3220 7840-7848 JJ denotes internal
T3219 7837-7839 DT denotes an
T3218 7834-7836 IN denotes as
T3217 7829-7833 VB denotes used
T3216 7825-7828 VB denotes was
T3215 7817-7824 NN denotes β-actin
T3214 7814-7816 IN denotes of
T3213 7808-7813 NN denotes level
T3212 7804-7807 DT denotes The
T3211 7795-7802 NN denotes program
T3210 7788-7794 NN denotes ImageJ
T3209 7785-7787 IN denotes by
T3208 7776-7784 VB denotes analyzed
T3207 7772-7775 VB denotes was
T3206 7766-7771 NN denotes bands
T3205 7762-7765 DT denotes the
T3204 7759-7761 IN denotes of
T3203 7749-7758 NN denotes intensity
T3202 7745-7748 DT denotes The
T3201 7728-7743 NN denotes electrophoresis
T3200 7724-7727 NN denotes gel
T3199 7716-7723 NN denotes agarose
T3198 7713-7715 IN denotes by
T3197 7704-7712 VB denotes detected
T3196 7699-7703 VB denotes were
T3195 7690-7698 NN denotes products
T3194 7686-7689 NN denotes PCR
T3193 7674-7684 NN denotes parameters
T3192 7670-7673 NN denotes PCR
T3191 7666-7669 CC denotes and
T3190 7658-7665 NN denotes primers
T3189 7654-7657 DT denotes the
T3188 7648-7653 VB denotes shows
T3187 7646-7647 CD denotes 1
T3186 7640-7645 JJ denotes Table
T3185 7629-7637 NN denotes protocol
T3184 7626-7628 POS denotes 's
T3183 7614-7626 NN denotes manufacturer
T3182 7610-7613 DT denotes the
T3181 7607-7609 TO denotes to
T3180 7597-7606 VB denotes according
T3179 7595-7596 -RRB- denotes )
T3178 7592-7595 NNP denotes USA
T3177 7590-7591 -COMMA- denotes ,
T3176 7588-7590 NNP denotes WI
T3175 7586-7587 -COMMA- denotes ,
T3174 7579-7586 NNP denotes Promega
T3173 7578-7579 -LRB- denotes (
T3172 7574-7577 NNP denotes Mix
T3171 7567-7573 NNP denotes Master
T3170 7561-7566 NNP denotes Green
T3169 7555-7560 NNP denotes GoTaq
T3168 7550-7554 IN denotes with
T3167 7540-7549 VB denotes conducted
T3166 7536-7539 VB denotes was
T3165 7522-7535 NN denotes transcription
T3164 7514-7521 JJ denotes Reverse
T3163 7507-7512 NN denotes −20°C
T3162 7504-7506 IN denotes in
T3161 7497-7503 VB denotes stored
T3160 7493-7496 VB denotes was
T3159 7488-7492 NN denotes cDNA
T3158 7484-7487 DT denotes The
T3157 7474-7482 NN denotes protocol
T3156 7471-7473 POS denotes 's
T3155 7459-7471 NN denotes manufacturer
T3154 7455-7458 DT denotes the
T3153 7452-7454 TO denotes to
T3152 7442-7451 VB denotes according
T3151 7440-7441 -RRB- denotes )
T3150 7435-7440 NNP denotes China
T3149 7433-7434 -COMMA- denotes ,
T3148 7426-7433 NNP denotes Jiangsu
T3147 7424-7425 -COMMA- denotes ,
T3146 7416-7424 NNP denotes Biunique
T3145 7415-7416 -LRB- denotes (
T3144 7408-7414 NN denotes RT-Kit
T3143 7398-7407 JJ denotes BU-Script
T3142 7393-7397 IN denotes with
T3141 7389-7392 NN denotes RNA
T3140 7383-7388 JJ denotes total
T3139 7380-7382 IN denotes of
T3138 7377-7379 NN denotes μg
T3137 7375-7376 CD denotes 2
T3136 7370-7374 IN denotes from
T3135 7358-7369 VB denotes synthesized
T3134 7354-7357 VB denotes was
T3133 7349-7353 NN denotes cDNA
T3132 7333-7348 JJ denotes single-stranded
T3131 7329-7332 CC denotes and
T3130 7327-7328 -COMMA- denotes ,
T3129 7326-7327 -RRB- denotes )
T3128 7323-7326 NNP denotes USA
T3127 7321-7322 -COMMA- denotes ,
T3126 7319-7321 NNP denotes CA
T3125 7317-7318 -COMMA- denotes ,
T3124 7307-7317 NN denotes Invitrogen
T3123 7306-7307 -LRB- denotes (
T3122 7299-7305 NN denotes Trizol
T3121 7294-7298 IN denotes with
T3120 7285-7293 VB denotes isolated
T3119 7281-7284 VB denotes was
T3118 7277-7280 NN denotes RNA
T3117 7271-7276 JJ denotes Total
T3116 7264-7270 NN denotes RT-PCR
T3115 7259-7262 CD denotes 2.5
T3479 8261-8271 NN denotes micrograms
T3478 8255-8260 CD denotes Fifty
T3477 8252-8253 -RRB- denotes )
T3476 8249-8252 NNP denotes USA
T3475 8247-8248 -COMMA- denotes ,
T3474 8245-8247 NNP denotes IL
T3473 8243-8244 -COMMA- denotes ,
T3472 8237-8243 NNP denotes Pierce
T3471 8236-8237 -LRB- denotes (
T3470 8228-8235 NNP denotes Reagent
T3469 8222-8227 NNP denotes Assay
T3468 8214-8221 NNP denotes Protein
T3467 8209-8213 NNP denotes Plus
T3466 8199-8208 NNP denotes Coomassie
T3465 8196-8198 IN denotes by
T3464 8186-8195 VB denotes estimated
T3463 8181-8185 VB denotes were
T3462 8166-8180 NN denotes concentrations
T3461 8158-8165 NN denotes Protein
T3460 8153-8156 NN denotes 4°C
T3459 8150-8152 IN denotes at
T3458 8146-8149 NN denotes min
T3457 8143-8145 CD denotes 15
T3456 8139-8142 IN denotes for
T3455 8137-8138 NN denotes g
T3454 8130-8136 CD denotes 12,000
T3453 8127-8129 IN denotes at
T3452 8115-8126 VB denotes centrifuged
T3451 8111-8114 CC denotes and
T3450 8109-8110 -RRB- denotes )
T3449 8106-8109 CD denotes 7.4
T3448 8103-8105 NN denotes pH
T3447 8099-8102 NNP denotes PBS
T3446 8096-8098 IN denotes in
T3445 8087-8095 NN denotes cocktail
T3444 8077-8086 NN denotes inhibitor
T3443 8068-8076 NN denotes protease
T3442 8064-8067 CC denotes and
T3441 8062-8063 -COMMA- denotes ,
T3440 8058-8062 NN denotes PMSF
T3439 8055-8057 NN denotes mM
T3438 8053-8054 CD denotes 1
T3437 8051-8052 -COMMA- denotes ,
T3436 8048-8051 NN denotes DTT
T3435 8045-8047 NN denotes mM
T3434 8041-8044 CD denotes 0.5
T3433 8039-8040 -COMMA- denotes ,
T3432 8036-8039 NN denotes NaF
T3431 8033-8035 NN denotes mM
T3430 8030-8032 CD denotes 20
T3429 8028-8029 -COMMA- denotes ,
T3428 8022-8028 NN denotes Na3VO4
T3427 8019-8021 NN denotes mM
T3426 8017-8018 CD denotes 1
T3425 8015-8016 -COMMA- denotes ,
T3424 8011-8015 NN denotes EGTA
T3423 8008-8010 NN denotes mM
T3422 8006-8007 CD denotes 1
T3421 8004-8005 -COMMA- denotes ,
T3420 8000-8004 NN denotes EDTA
T3419 7997-7999 NN denotes mM
T3418 7995-7996 CD denotes 1
T3417 7993-7994 -COMMA- denotes ,
T3416 7990-7993 NN denotes SDS
T3415 7988-7989 NN denotes %
T3414 7985-7988 CD denotes 0.1
T3413 7983-7984 -COMMA- denotes ,
T3412 7971-7983 NN denotes deoxycholate
T3411 7964-7970 NN denotes sodium
T3410 7962-7963 NN denotes %
T3409 7959-7962 CD denotes 0.5
T3408 7957-7958 -COMMA- denotes ,
T3407 7953-7957 NN denotes NP40
T3406 7951-7952 NN denotes %
T3405 7950-7951 CD denotes 1
T3404 7949-7950 -LRB- denotes (
T3403 7942-7948 NN denotes buffer
T3402 7937-7941 NN denotes RIPA
T3401 7934-7936 IN denotes in
T3400 7922-7933 VB denotes homogenized
T3399 7917-7921 VB denotes were
T3398 7911-7916 NN denotes cells
T3397 7909-7910 -COMMA- denotes ,
T3396 7902-7909 NN denotes lysates
T3395 7894-7901 NN denotes protein
T3394 7888-7893 JJ denotes total
T3393 7881-7887 VB denotes obtain
T3392 7878-7880 TO denotes To
T3391 7873-7877 NNP denotes Blot
T3390 7865-7872 NNP denotes Western
T3389 7860-7863 CD denotes 2.6
T2662 7250-7256 NN denotes ImageJ
T2661 7241-7249 NN denotes software
T2660 7238-7240 IN denotes by
T2659 7227-7237 VB denotes quantified
T2658 7222-7226 VB denotes were
T2657 7213-7221 NN denotes activity
T2656 7205-7212 NN denotes binding
T2655 7201-7204 NN denotes DNA
T2654 7195-7200 NN denotes NF-κB
T2653 7192-7194 IN denotes of
T2652 7185-7191 NN denotes Levels
T2651 7182-7183 -RRB- denotes )
T2650 7177-7182 NNP denotes Japan
T2649 7175-7176 -COMMA- denotes ,
T2648 7170-7175 NNP denotes Tokyo
T2647 7168-7169 -COMMA- denotes ,
T2646 7159-7168 NNP denotes Hyperfilm
T2645 7154-7158 NNP denotes Fuji
T2644 7153-7154 -LRB- denotes (
T2643 7148-7152 NN denotes film
T2642 7142-7147 NN denotes X-ray
T2641 7139-7141 TO denotes to
T2640 7131-7138 VB denotes exposed
T2639 7127-7130 CC denotes and
T2638 7121-7126 VB denotes dried
T2637 7117-7120 VB denotes was
T2636 7113-7116 NN denotes gel
T2635 7109-7112 DT denotes the
T2634 7107-7108 -COMMA- denotes ,
T2633 7092-7107 NN denotes electrophoresis
T2632 7086-7091 IN denotes After
T2631 7081-7084 NN denotes 4°C
T2630 7078-7080 IN denotes at
T2629 7073-7077 NN denotes hour
T2628 7071-7072 CD denotes 1
T2627 7067-7070 IN denotes for
T2626 7065-7066 NN denotes V
T2625 7061-7064 CD denotes 390
T2624 7058-7060 IN denotes at
T2623 7056-7057 -RRB- denotes )
T2622 7040-7056 NN denotes tris-borate-EDTA
T2621 7039-7040 -LRB- denotes (
T2620 7032-7038 NN denotes buffer
T2619 7028-7031 NN denotes TBE
T2618 7026-7027 SYM denotes ×
T2617 7022-7025 CD denotes 0.5
T2616 7019-7021 IN denotes in
T2615 7015-7018 NN denotes gel
T2614 7000-7014 NN denotes polyacrylamide
T2613 6986-6999 JJ denotes nondenaturing
T2612 6984-6985 NN denotes %
T2611 6983-6984 CD denotes 4
T2610 6980-6982 IN denotes on
T2609 6964-6979 NN denotes electrophoresis
T2608 6961-6963 IN denotes by
T2607 6951-6960 VB denotes separated
T2606 6946-6950 VB denotes were
T2605 6936-6945 NN denotes complexes
T2604 6924-6935 JJ denotes DNA-protein
T2603 6920-6923 DT denotes The
T2602 6915-6918 NN denotes ice
T2601 6912-6914 IN denotes on
T2600 6904-6911 NN denotes minutes
T2599 6901-6903 CD denotes 20
T2598 6897-6900 IN denotes for
T2597 6887-6896 VB denotes continued
T2596 6883-6886 VB denotes was
T2595 6872-6882 NN denotes incubation
T2594 6868-6871 DT denotes the
T2593 6866-6867 -COMMA- denotes ,
T2592 6861-6866 NN denotes probe
T2591 6845-6860 NN denotes oligonucleotide
T2590 6834-6844 JJ denotes 32P-labled
T2589 6831-6833 NN denotes μL
T2588 6829-6830 CD denotes 1
T2587 6825-6828 DT denotes the
T2586 6822-6824 IN denotes of
T2585 6813-6821 NN denotes addition
T2584 6807-6812 IN denotes After
T2583 6802-6805 NN denotes ice
T3488 8336-8338 IN denotes in
T3487 8321-8335 JJ denotes heat-denatured
T3486 8316-8320 VB denotes were
T3485 8307-8315 NN denotes extracts
T3484 8299-8306 NN denotes protein
T3483 8289-8298 JJ denotes cytosolic
T3482 8279-8288 VB denotes resulting
T3481 8275-8278 DT denotes the
T3480 8272-8274 IN denotes of
T2582 6799-6801 IN denotes on
T2581 6791-6798 NN denotes minutes
T2580 6788-6790 CD denotes 20
T2579 6784-6787 IN denotes for
T2578 6778-6783 NN denotes dI-dC
T2577 6773-6777 NN denotes poly
T2576 6770-6772 NN denotes μg
T2575 6768-6769 CD denotes 2
T2574 6764-6767 CC denotes and
T2573 6762-6763 -COMMA- denotes ,
T2572 6759-6762 NN denotes DTT
T2571 6756-6758 NN denotes mM
T2570 6752-6755 CD denotes 0.5
T2569 6750-6751 -COMMA- denotes ,
T2568 6746-6750 NN denotes EDTA
T2567 6743-6745 NN denotes mM
T2566 6739-6742 CD denotes 0.5
T2565 6737-6738 -COMMA- denotes ,
T2564 6729-6737 NN denotes glycerol
T2563 6727-6728 NN denotes %
T2562 6726-6727 CD denotes 4
T2561 6724-6725 -COMMA- denotes ,
T2560 6720-6724 NN denotes NaCl
T2559 6717-6719 NN denotes mM
T2558 6713-6716 CD denotes 0.5
T2557 6711-6712 -COMMA- denotes ,
T2493 6384-6389 VB denotes using
T2492 6374-6383 VB denotes performed
T2491 6370-6373 VB denotes was
T2490 6365-6369 NN denotes EMSA
T2489 6362-6363 -RRB- denotes )
T2488 6359-6362 NNP denotes USA
T2487 6357-6358 -COMMA- denotes ,
T2486 6355-6357 NNP denotes IL
T2485 6353-6354 -COMMA- denotes ,
T2484 6345-6353 NNP denotes Rockford
T2483 6343-6344 -COMMA- denotes ,
T2482 6331-6343 NNP denotes Biochemicals
T2481 6324-6330 NNP denotes Pierce
T2480 6323-6324 -LRB- denotes (
T2479 6314-6322 JJ denotes standard
T2478 6310-6313 DT denotes the
T2477 6307-6309 IN denotes as
T2476 6299-6306 NN denotes albumin
T2475 6293-6298 NN denotes serum
T2474 6286-6292 JJ denotes bovine
T2473 6281-6285 IN denotes with
T2472 6277-6280 NN denotes kit
T2471 6271-6276 NN denotes assay
T2470 6266-6270 NN denotes acid
T2469 6252-6265 JJ denotes bicinchoninic
T2468 6250-6251 DT denotes a
T2467 6244-6249 VB denotes using
T2466 6233-6243 VB denotes determined
T2465 6229-6232 VB denotes was
T2464 6215-6228 NN denotes concentration
T2463 6207-6214 NN denotes Protein
T2462 6202-6205 NN denotes use
T2461 6196-6201 IN denotes until
T2460 6190-6195 NN denotes –80°C
T2459 6187-6189 IN denotes at
T2458 6180-6186 VB denotes stored
T2457 6176-6179 VB denotes was
T2456 6167-6175 NN denotes proteins
T2455 6159-6166 JJ denotes nuclear
T2454 6155-6158 DT denotes the
T2453 6144-6154 VB denotes containing
T2452 6132-6143 NN denotes supernatant
T2451 6128-6131 DT denotes the
T2450 6124-6127 CC denotes and
T2449 6122-6123 -COMMA- denotes ,
T2448 6119-6122 NN denotes rpm
T2447 6113-6118 CD denotes 14000
T2446 6110-6112 IN denotes at
T2445 6095-6109 NN denotes centrifugation
T2444 6092-6094 IN denotes by
T2443 6084-6091 VB denotes removed
T2442 6080-6083 VB denotes was
T2441 6071-6079 NN denotes material
T2440 6061-6070 JJ denotes Insoluble
T2439 6051-6059 NN denotes fluoride
T2438 6030-6050 NN denotes phenylmethylsulfonyl
T2437 6027-6029 NN denotes mM
T2436 6023-6026 CD denotes 1.0
T2435 6019-6022 CC denotes and
T2434 6017-6018 -COMMA- denotes ,
T2433 6014-6017 NN denotes DTT
T2432 6011-6013 NN denotes mM
T2431 6007-6010 CD denotes 1.0
T2430 6005-6006 -COMMA- denotes ,
T2429 6001-6005 NN denotes EGTA
T2428 5998-6000 NN denotes mM
T2427 5994-5997 CD denotes 1.0
T2426 5992-5993 -COMMA- denotes ,
T2425 5988-5992 NN denotes EDTA
T2424 5985-5987 NN denotes mM
T2423 5981-5984 CD denotes 1.0
T2422 5979-5980 -COMMA- denotes ,
T2421 5975-5979 NN denotes NaCl
T2420 5972-5974 NN denotes mM
T2419 5968-5971 CD denotes 0.4
T2418 5966-5967 -COMMA- denotes ,
T2417 5965-5966 -RRB- denotes )
T2416 5962-5965 CD denotes 7.9
T2415 5959-5961 NN denotes pH
T2414 5958-5959 -LRB- denotes (
T2413 5952-5957 NN denotes HEPES
T2412 5949-5951 NN denotes mM
T2411 5946-5948 CD denotes 20
T2410 5935-5945 VB denotes containing
T2409 5928-5934 NN denotes buffer
T2408 5923-5927 IN denotes with
T2407 5913-5922 VB denotes extracted
T2406 5908-5912 VB denotes were
T2405 5899-5907 NN denotes proteins
T2404 5891-5898 JJ denotes nuclear
T2403 5887-5890 DT denotes The
T2402 5871-5885 NN denotes centrifugation
T2401 5868-5870 IN denotes by
T2400 5859-5867 VB denotes pelleted
T2399 5854-5858 VB denotes were
T2398 5847-5853 NN denotes nuclei
T2397 5843-5846 DT denotes the
T2396 5839-5842 CC denotes and
T2395 5837-5838 -COMMA- denotes ,
T2394 5830-5837 NN denotes briefly
T2393 5821-5829 VB denotes vortexed
T2392 5817-5820 CC denotes and
T2391 5811-5816 VB denotes added
T2390 5807-5810 VB denotes was
T2389 5802-5806 NN denotes P-40
T2388 5794-5801 NN denotes Nonidet
T2387 5792-5793 NN denotes %
T2386 5790-5792 CD denotes 10
T2385 5785-5789 RB denotes Then
T2384 5775-5783 NN denotes fluoride
T2383 5754-5774 NN denotes phenylmethylsulfonyl
T2382 5751-5753 NN denotes mM
T2381 5747-5750 CD denotes 0.5
T2380 5743-5746 CC denotes and
T2379 5741-5742 -COMMA- denotes ,
T2378 5738-5741 NN denotes DTT
T2377 5735-5737 NN denotes mM
T2376 5731-5734 CD denotes 1.0
T2375 5729-5730 -COMMA- denotes ,
T2374 5725-5729 NN denotes EGTA
T2373 5722-5724 NN denotes mM
T2372 5718-5721 CD denotes 0.1
T2371 5716-5717 -COMMA- denotes ,
T2370 5712-5716 NN denotes EDTA
T2369 5709-5711 NN denotes mM
T2368 5705-5708 CD denotes 0.1
T2367 5703-5704 -COMMA- denotes ,
T2366 5700-5703 NN denotes KCl
T2365 5697-5699 NN denotes mM
T2364 5694-5696 CD denotes 10
T2363 5692-5693 -COMMA- denotes ,
T2362 5691-5692 -RRB- denotes )
T2361 5688-5691 CD denotes 7.9
T2360 5685-5687 NN denotes pH
T2359 5684-5685 -LRB- denotes (
T2358 5678-5683 NN denotes HEPES
T2357 5675-5677 NN denotes mM
T2356 5672-5674 CD denotes 10
T2355 5661-5671 VB denotes containing
T2354 5654-5660 NN denotes buffer
T2353 5651-5653 IN denotes in
T2352 5639-5650 VB denotes resuspended
T2351 5635-5638 VB denotes was
T2350 5620-5634 NN denotes centrifugation
T2349 5617-5619 IN denotes by
T2348 5608-5616 VB denotes obtained
T2347 5601-5607 NN denotes pellet
T2346 5596-5600 NN denotes cell
T2345 5592-5595 DT denotes The
T2344 5587-5590 NNP denotes PBS
T2343 5582-5586 JJ denotes cold
T2342 5577-5581 IN denotes into
T2341 5568-5576 VB denotes scraping
T2340 5565-5567 IN denotes by
T2339 5555-5564 VB denotes harvested
T2338 5551-5554 CC denotes and
T2337 5547-5550 NNP denotes PBS
T2336 5542-5546 IN denotes with
T2335 5535-5541 VB denotes washed
T2334 5530-5534 VB denotes were
T2333 5519-5529 NN denotes astrocytes
T2332 5516-5518 IN denotes of
T2331 5505-5515 NN denotes Monolayers
T2330 5503-5504 -RRB- denotes )
T2329 5499-5503 NN denotes EMSA
T2328 5498-5499 -LRB- denotes (
T2327 5492-5497 NN denotes Assay
T2326 5486-5491 NN denotes Shift
T2325 5477-5485 NN denotes Mobility
T2324 5461-5476 JJ denotes Electrophoretic
T2323 5456-5459 CD denotes 2.4
T2162 5451-5453 NN denotes SD
T2161 5449-5450 SYM denotes ±
T2160 5446-5448 NN denotes nm
T2159 5442-5445 CD denotes 450
T2158 5439-5441 IN denotes at
T2157 5433-5438 NN denotes value
T2156 5430-5432 NN denotes OD
T2155 5427-5429 IN denotes of
T2154 5422-5426 NN denotes mean
T2153 5419-5421 IN denotes as
T2152 5409-5418 VB denotes expressed
T2151 5404-5408 VB denotes were
T2150 5396-5403 NN denotes Results
T2149 5382-5394 NN denotes sextuplicate
T2148 5379-5381 IN denotes in
T2147 5369-5378 VB denotes performed
T2146 5364-5368 VB denotes were
T2145 5351-5363 NN denotes measurements
T2144 5347-5350 DT denotes All
T2143 5344-5345 -RRB- denotes )
T2142 5341-5344 NNP denotes USA
T2141 5339-5340 -COMMA- denotes ,
T2140 5337-5339 NNP denotes CA
T2139 5335-5336 -COMMA- denotes ,
T2138 5323-5335 NNP denotes Laboratories
T2137 5315-5322 NNP denotes Bio-Rad
T2136 5314-5315 -LRB- denotes (
T2135 5307-5313 NN denotes reader
T2134 5296-5306 NN denotes microplate
T2133 5290-5295 NN denotes ELISA
T2132 5282-5289 JJ denotes Bio-Rad
T2131 5280-5281 DT denotes a
T2130 5274-5279 VB denotes using
T2129 5271-5273 NN denotes nm
T2128 5267-5270 CD denotes 450
T2127 5264-5266 IN denotes at
T2126 5259-5263 VB denotes read
T2125 5255-5258 VB denotes was
T2124 5249-5254 NN denotes value
T2123 5246-5248 NN denotes OD
T2122 5241-5245 RB denotes Then
T2121 5238-5239 NN denotes h
T2120 5236-5237 CD denotes 1
T2119 5232-5235 IN denotes for
T2118 5222-5231 VB denotes incubated
T2117 5218-5221 CC denotes and
T2116 5213-5217 RB denotes well
T2115 5207-5212 DT denotes every
T2114 5202-5206 IN denotes into
T2113 5196-5201 VB denotes added
T2112 5192-5195 VB denotes was
T2111 5186-5191 NN denotes CCK-8
T2110 5183-5185 NN denotes μL
T2109 5180-5182 CD denotes 10
T2108 5178-5179 -COMMA- denotes ,
T2107 5171-5178 NNP denotes Briefly
T2106 5161-5169 NN denotes protocol
T2105 5158-5160 POS denotes 's
T2104 5146-5158 NN denotes manufacturer
T2103 5142-5145 DT denotes the
T2102 5139-5141 TO denotes to
T2101 5129-5138 VB denotes according
T2100 5121-5128 NN denotes scratch
T2099 5115-5120 IN denotes after
T2098 5113-5114 NN denotes h
T2097 5110-5112 CD denotes 24
T2096 5104-5109 NN denotes assay
T2095 5102-5103 -RRB- denotes )
T2094 5097-5102 NNP denotes Japan
T2093 5095-5096 -COMMA- denotes ,
T2092 5088-5095 NNP denotes Dojindo
T2091 5087-5088 -LRB- denotes (
T2090 5085-5086 -RRB- denotes )
T2089 5080-5085 NN denotes CCK-8
T2088 5079-5080 -LRB- denotes (
T2087 5073-5078 NN denotes kit-8
T2086 5064-5072 NN denotes counting
T2085 5059-5063 NN denotes cell
T2084 5056-5058 IN denotes by
T2083 5047-5055 VB denotes assessed
T2082 5043-5046 VB denotes was
T2081 5037-5042 NN denotes death
T2080 5032-5036 NN denotes cell
T2079 5022-5031 NN denotes Astrocyte
T2078 5015-5020 IN denotes above
T2077 5005-5014 VB denotes mentioned
T2076 5002-5004 IN denotes as
T2075 4994-5001 VB denotes scrated
T2074 4990-4993 VB denotes was
T2073 4985-4989 NN denotes cell
T2072 4981-4984 DT denotes the
T2071 4976-4980 RB denotes Then
T2556 6706-6711 NN denotes MgCl2
T2555 6703-6705 NN denotes mM
T2554 6701-6702 CD denotes 1
T2553 6699-6700 -COMMA- denotes ,
T2552 6698-6699 -RRB- denotes )
T2551 6695-6698 CD denotes 7.5
T2550 6692-6694 NN denotes PH
T2549 6691-6692 -LRB- denotes (
T2548 6682-6690 NN denotes Tris-HCl
T2547 6679-6681 NN denotes mM
T2546 6676-6678 CD denotes 10
T2545 6665-6675 VB denotes containing
T2544 6658-6664 NN denotes buffer
T2543 6650-6657 NN denotes binding
T2542 6647-6649 NN denotes μL
T2541 6644-6646 CD denotes 20
T2540 6641-6643 IN denotes in
T2539 6628-6640 VB denotes preincubated
T2538 6624-6627 VB denotes was
T2537 6622-6623 -RRB- denotes )
T2536 6620-6622 NN denotes μg
T2535 6617-6619 CD denotes 20
T2534 6616-6617 -LRB- denotes (
T2533 6608-6615 NN denotes protein
T2532 6600-6607 JJ denotes Nuclear
T2531 6597-6598 -RRB- denotes )
T2530 6592-6597 NNP denotes China
T2529 6590-6591 -COMMA- denotes ,
T2528 6583-6590 NNP denotes Beijing
T2527 6581-6582 -COMMA- denotes ,
T2526 6573-6581 NNP denotes Biotech.
T2525 6568-6572 NNP denotes Free
T2524 6567-6568 -LRB- denotes (
T2523 6562-6566 NN denotes -ATP
T2522 6561-6562 -RRB- denotes ]
T2521 6556-6561 NN denotes γ-32P
T2520 6555-6556 -LRB- denotes [
T2519 6549-6554 VB denotes using
T2518 6542-6548 NN denotes kinase
T2517 6524-6541 NN denotes T4-polynucleotide
T2516 6521-6523 IN denotes by
T2515 6509-6520 VB denotes end-labeled
T2514 6505-6508 VB denotes was
T2513 6503-6504 -RRB- denotes )
T2512 6502-6503 -DQE- denotes ′
T2511 6475-6502 NN denotes 5′-AGTTGAGGGGACTTTCCCAGGG-3
T2510 6474-6475 -LRB- denotes (
T2509 6468-6473 NN denotes probe
T2508 6452-6467 NN denotes oligonucleotide
T2507 6442-6451 NNP denotes Consensus
T2506 6439-6440 -RRB- denotes )
T2505 6436-6439 NNP denotes USA
T2504 6434-6435 -COMMA- denotes ,
T2503 6432-6434 NNP denotes WI
T2502 6430-6431 -COMMA- denotes ,
T2501 6423-6430 NNP denotes Madison
T2500 6421-6422 -COMMA- denotes ,
T2499 6414-6421 NNP denotes Promega
T2498 6413-6414 -LRB- denotes (
T2497 6406-6412 NN denotes system
T2496 6400-6405 NN denotes assay
T2495 6394-6399 NN denotes shift
T2494 6390-6393 NN denotes gel
T2070 4968-4974 VB denotes adhere
T2069 4965-4967 TO denotes to
T2068 4963-4964 NN denotes h
T2067 4960-4962 CD denotes 24
T2066 4956-4959 IN denotes for
T2065 4945-4955 VB denotes cultivated
T2064 4941-4944 CC denotes and
T2063 4934-4940 NN denotes plates
T2062 4926-4933 NN denotes culture
T2061 4918-4925 JJ denotes 96-well
T2060 4915-4917 TO denotes to
T2059 4908-4914 VB denotes seeded
T2058 4903-4907 VB denotes were
T2057 4897-4902 NN denotes cells
T2056 4888-4896 NN denotes 103/well
T2055 4886-4887 SYM denotes ×
T2054 4884-4885 CD denotes 1
T2053 4878-4883 NN denotes Assay
T2052 4872-4877 NN denotes CCK-8
T2051 4869-4871 IN denotes by
T2050 4857-4868 NNP denotes Measurement
T2049 4851-4856 NNP denotes Death
T2048 4846-4850 NNP denotes Cell
T2047 4841-4844 CD denotes 2.3
T1499 4299-4306 NN denotes studies
T1498 4289-4298 VB denotes following
T1497 4285-4288 DT denotes the
T1496 4282-4284 IN denotes in
T1495 4277-4281 VB denotes used
T1494 4272-4276 VB denotes were
T1493 4260-4271 NN denotes generations
T1492 4256-4259 CD denotes 2-3
T1491 4252-4255 IN denotes for
T1490 4243-4251 VB denotes passaged
T1489 4237-4242 NN denotes Cells
T1488 4234-4235 -RRB- denotes )
T1487 4231-4234 NNP denotes USA
T1486 4229-4230 -COMMA- denotes ,
T1485 4227-4229 NNP denotes CA
T1484 4225-4226 -COMMA- denotes ,
T1483 4212-4225 NNP denotes Biotechnology
T1482 4207-4211 NNP denotes Cruz
T1481 4201-4206 NNP denotes Santa
T1480 4199-4200 -COMMA- denotes ,
T1479 4190-4199 NN denotes sc-166481
T1478 4189-4190 -LRB- denotes (
T1477 4184-4188 NN denotes GFAP
T1476 4180-4183 IN denotes for
T1475 4163-4179 NN denotes immunoreactivity
T1474 4160-4162 IN denotes by
T1473 4149-4159 VB denotes determined
T1472 4144-4148 VB denotes were
T1471 4138-4143 WDT denotes which
T1470 4136-4137 -COMMA- denotes ,
T1469 4126-4136 NN denotes astrocytes
T1468 4119-4125 RB denotes mainly
T1467 4114-4118 VB denotes were
T1466 4105-4113 NN denotes cultures
T1465 4095-4104 VB denotes resulting
T1464 4091-4094 DT denotes the
T1463 4089-4090 -COMMA- denotes ,
T1462 4082-4089 VB denotes shaking
T1461 4076-4081 IN denotes After
T1460 4066-4074 NN denotes cultures
T1459 4062-4065 DT denotes the
T1458 4057-4061 IN denotes from
T1457 4051-4056 NN denotes cells
T1456 4042-4050 JJ denotes adherent
T1455 4037-4041 RB denotes less
T1454 4033-4036 CC denotes and
T1453 4023-4032 NN denotes microglia
T1452 4019-4022 DT denotes the
T1451 4011-4018 VB denotes deplete
T1450 4008-4010 TO denotes to
T1449 4006-4007 NN denotes h
T1448 4004-4005 CD denotes 4
T1447 4000-4003 IN denotes for
T1446 3996-3999 NN denotes rpm
T1445 3992-3995 CD denotes 150
T1444 3989-3991 IN denotes at
T1443 3982-3988 VB denotes shaken
T1442 3977-3981 VB denotes were
T1441 3970-3976 NN denotes dishes
T1440 3967-3969 CC denotes or
T1439 3960-3966 NN denotes flasks
T1438 3956-3959 DT denotes the
T1437 3951-3955 RB denotes Then
T1436 3939-3949 NN denotes confluence
T1435 3933-3938 VB denotes reach
T1434 3930-3932 TO denotes to
T1433 3925-3929 NN denotes days
T1432 3923-3924 CD denotes 7
T1431 3917-3922 IN denotes about
T1430 3913-3916 IN denotes for
T1429 3904-3912 VB denotes expanded
T1428 3893-3903 NN denotes Astrocytes
T1427 3887-3891 NN denotes week
T1426 3885-3886 DT denotes a
T1425 3879-3884 RB denotes twice
T1424 3866-3878 JJ denotes half-changed
T1423 3862-3865 VB denotes was
T1422 3855-3861 NN denotes medium
T1421 3847-3854 NN denotes culture
T1420 3838-3846 JJ denotes complete
T1419 3834-3837 DT denotes the
T1418 3830-3833 CC denotes And
T1417 3825-3828 NN denotes CO2
T1416 3823-3824 NN denotes %
T1415 3822-3823 CD denotes 5
T1414 3817-3821 IN denotes with
T1413 3812-3816 NN denotes 37°C
T1412 3809-3811 IN denotes at
T1411 3798-3808 VB denotes cultivated
T1410 3793-3797 VB denotes were
T1409 3787-3792 NN denotes cells
T1408 3783-3786 DT denotes the
T1407 3778-3782 RB denotes Then
T1406 3771-3776 NN denotes cells
T1405 3760-3770 JJ denotes epithelial
T1404 3751-3759 JJ denotes residual
T1403 3747-3750 DT denotes the
T1402 3737-3746 VB denotes depleting
T1401 3733-3736 IN denotes for
T1400 3731-3732 -RRB- denotes )
T1399 3728-3731 NNP denotes USA
T1398 3726-3727 -COMMA- denotes ,
T1397 3720-3726 NNP denotes Costar
T1396 3719-3720 -LRB- denotes (
T1395 3714-3718 JJ denotes dish
T1394 3711-3713 CC denotes or
T1393 3705-3710 NN denotes flask
T1392 3701-3704 JJ denotes new
T1391 3699-3700 DT denotes a
T1390 3696-3698 TO denotes to
T1389 3684-3695 VB denotes transferred
T1388 3680-3683 VB denotes was
T1387 3668-3679 NN denotes supernatant
T1386 3664-3667 DT denotes the
T1385 3662-3663 -COMMA- denotes ,
T1384 3661-3662 NN denotes h
T1383 3659-3660 CD denotes 1
T1382 3655-3658 IN denotes for
T1381 3644-3654 NN denotes incubation
T1380 3638-3643 IN denotes After
T1379 3635-3636 -RRB- denotes )
T1378 3629-3635 NN denotes medium
T1377 3621-3628 NN denotes culture
T1376 3612-3620 JJ denotes complete
T1375 3611-3612 -LRB- denotes (
T1374 3598-3610 NN denotes streptomycin
T1373 3592-3597 NN denotes μg/mL
T1372 3590-3591 CD denotes 5
T1371 3588-3589 -COMMA- denotes ,
T1370 3578-3588 NN denotes penicillin
T1369 3569-3577 NN denotes units/mL
T1368 3567-3568 CD denotes 5
T1367 3563-3566 CC denotes and
T1366 3559-3562 NN denotes FBS
T1365 3557-3558 NN denotes %
T1364 3555-3557 CD denotes 20
T1363 3550-3554 IN denotes with
T1362 3545-3549 NN denotes DMEM
T1361 3542-3544 IN denotes in
T1360 3529-3541 NN denotes dissociation
T1359 3518-3528 JJ denotes mechanical
T1358 3516-3517 DT denotes a
T1357 3513-3515 IN denotes by
T1356 3504-3512 VB denotes followed
T1355 3502-3503 -COMMA- denotes ,
T1354 3499-3502 NNP denotes FBS
T1353 3497-3498 NN denotes %
T1352 3495-3497 CD denotes 10
T1351 3490-3494 IN denotes with
T1350 3485-3489 NN denotes DMEM
T1349 3482-3484 IN denotes in
T1348 3476-3481 RB denotes twice
T1347 3469-3475 VB denotes rinsed
T1346 3465-3468 VB denotes was
T1345 3458-3464 NN denotes tissue
T1344 3454-3457 DT denotes the
T1343 3452-3453 -COMMA- denotes ,
T1342 3443-3452 NN denotes digestion
T1341 3437-3442 IN denotes After
T1340 3428-3435 NN denotes minutes
T1339 3424-3427 JJ denotes few
T1338 3418-3423 DT denotes every
T1337 3409-3417 VB denotes agitated
T1336 3405-3408 CC denotes and
T1335 3403-3404 -COMMA- denotes ,
T1334 3399-3403 NN denotes 37°C
T1333 3396-3398 IN denotes at
T1332 3388-3395 NN denotes minutes
T1331 3385-3387 CD denotes 10
T1330 3381-3384 IN denotes for
T1329 3373-3380 NN denotes trypsin
T1328 3371-3372 NN denotes %
T1327 3368-3371 CD denotes 0.5
T1326 3365-3367 IN denotes in
T1325 3355-3364 VB denotes incubated
T1324 3353-3354 -COMMA- denotes ,
T1323 3348-3353 NN denotes blade
T1322 3340-3347 NN denotes scalpel
T1321 3338-3339 DT denotes a
T1320 3333-3337 IN denotes with
T1916 4833-4838 NN denotes group
T1915 4828-4832 NN denotes sham
T1914 4825-4827 IN denotes as
T1913 4819-4824 VB denotes named
T1912 4815-4818 CC denotes and
T1911 4807-4814 NN denotes control
T1910 4801-4806 JJ denotes blank
T1909 4798-4800 IN denotes as
T1908 4793-4797 VB denotes used
T1907 4788-4792 VB denotes were
T1906 4786-4787 -COMMA- denotes ,
T1905 4779-4786 NN denotes scratch
T1904 4770-4778 VB denotes received
T1903 4766-4769 RB denotes not
T1902 4762-4765 VB denotes did
T1901 4756-4761 WDT denotes which
T1900 4754-4755 -COMMA- denotes ,
T1899 4749-4754 NN denotes Cells
T1898 4742-4747 NN denotes group
T1897 4738-4741 NN denotes TBI
T1896 4735-4737 IN denotes as
T1895 4729-4734 VB denotes named
T1894 4725-4728 CC denotes and
T1893 4723-4724 -COMMA- denotes ,
T1892 4717-4723 NN denotes medium
T1891 4709-4716 NN denotes culture
T1890 4700-4708 JJ denotes complete
T1889 4695-4699 IN denotes with
T1888 4686-4694 VB denotes cultured
T1887 4684-4685 -COMMA- denotes ,
T1886 4681-4684 NNP denotes PBS
T1885 4673-4680 JJ denotes sterile
T1884 4668-4672 IN denotes with
T1883 4662-4667 RB denotes twice
T1882 4655-4661 VB denotes washed
T1881 4650-4654 VB denotes were
T1880 4644-4649 NN denotes cells
T1879 4642-4643 -COMMA- denotes ,
T1877 4629-4634 IN denotes after
T1876 4617-4628 RB denotes Immediately
T1875 4612-4615 NN denotes gap
T1874 4607-4611 JJ denotes wide
T1873 4604-4606 NN denotes mm
T1872 4600-4603 CD denotes 0.5
T1871 4598-4599 DT denotes a
T1870 4595-4597 IN denotes of
T1869 4585-4594 NN denotes formation
T1868 4581-4584 DT denotes the
T1867 4578-4580 IN denotes in
T1866 4568-4577 VB denotes resulting
T1865 4566-4567 -COMMA- denotes ,
T1864 4560-4566 NN denotes needle
T1863 4552-4559 NN denotes syringe
T1862 4548-4551 NN denotes 26G
T1861 4540-4547 JJ denotes sterile
T1860 4538-4539 DT denotes a
T1859 4533-4537 IN denotes with
T1858 4523-4532 VB denotes scratched
T1857 4519-4522 VB denotes was
T1856 4509-4518 NN denotes monolayer
T1855 4504-4508 NN denotes cell
T1854 4500-4503 DT denotes The
T1853 4488-4498 VB denotes confluence
T1852 4485-4487 TO denotes to
T1851 4479-4484 VB denotes grown
T1850 4475-4478 CC denotes and
T1849 4468-4474 NN denotes plates
T1848 4461-4467 JJ denotes 6-well
T1847 4458-4460 IN denotes in
T1846 4450-4457 VB denotes planted
T1845 4445-4449 VB denotes were
T1844 4434-4444 NN denotes Astrocytes
T1843 4431-4432 -RRB- denotes ]
T1842 4430-4431 CD denotes 7
T1841 4429-4430 -LRB- denotes [
T1840 4422-4428 NN denotes report
T1839 4413-4421 JJ denotes previous
T1838 4411-4412 DT denotes a
T1837 4408-4410 IN denotes in
T1836 4405-4407 IN denotes as
T1835 4395-4404 VB denotes performed
T1834 4391-4394 VB denotes was
T1833 4384-4390 NN denotes injury
T1832 4376-4383 NN denotes scratch
T1831 4366-4375 NN denotes Astrocyte
T1830 4359-4365 NNP denotes Injury
T1829 4351-4358 NNP denotes Scratch
T1828 4348-4350 IN denotes by
T1827 4336-4347 VB denotes Established
T1826 4332-4335 NNP denotes TBI
T1825 4329-4331 IN denotes of
T1824 4323-4328 NNP denotes Model
T1823 4317-4322 NNP denotes Vitro
T1822 4314-4316 IN denotes In
T1821 4309-4312 CD denotes 2.2
T1319 3325-3332 VB denotes chopped
T1318 3317-3324 RB denotes roughly
T1317 3313-3316 VB denotes was
T1316 3306-3312 NN denotes tissue
T1315 3302-3305 DT denotes The
T1314 3293-3300 VB denotes removed
T1313 3288-3292 VB denotes were
T1312 3280-3287 NN denotes vessels
T1311 3274-3279 NN denotes blood
T1310 3263-3273 VB denotes associated
T1309 3259-3262 CC denotes and
T1308 3250-3258 NN denotes meninges
T1307 3246-3249 DT denotes the
T1306 3242-3245 CC denotes and
T1305 3240-3241 -COMMA- denotes ,
T1304 3237-3240 RP denotes out
T1303 3227-3236 VB denotes dissected
T1302 3222-3226 VB denotes were
T1301 3213-3221 NN denotes cortices
T1300 3209-3212 DT denotes the
T1299 3207-3208 -COMMA- denotes ,
T1298 3195-3207 NN denotes decapitation
T1297 3185-3194 VB denotes Following
T1296 3182-3183 -RRB- denotes )
T1295 3174-3182 NN denotes genotype
T1294 3169-3173 DT denotes each
T1248 2939-2943 NN denotes Nrf2
T1247 2928-2938 NN denotes Astrocytes
T1246 2922-2927 NN denotes Mouse
T1245 2919-2921 IN denotes of
T1244 2904-2918 NN denotes Identification
T1243 2900-2903 CC denotes and
T1242 2892-2899 NN denotes Culture
T1241 2884-2891 JJ denotes Primary
T1240 2879-2882 CD denotes 2.1
T792 2224-2228 IN denotes than
T791 2219-2223 NN denotes mice
T790 2210-2218 NN denotes knockout
T789 2205-2209 NN denotes Nrf2
T788 2202-2204 IN denotes in
T787 2195-2201 NN denotes damage
T786 2184-2194 JJ denotes aggravated
T785 2179-2183 RB denotes more
T784 2172-2178 VB denotes induce
T783 2166-2171 MD denotes could
T782 2162-2165 NN denotes TBI
T781 2157-2161 IN denotes that
T780 2150-2156 VB denotes showed
T779 2145-2149 RB denotes also
T778 2141-2144 VB denotes was
T777 2138-2140 PRP denotes It
T776 2135-2136 -RRB- denotes )
T775 2132-2135 NN denotes TBI
T774 2131-2132 -LRB- denotes (
T773 2124-2130 NN denotes injury
T772 2118-2123 NN denotes brain
T771 2108-2117 JJ denotes traumatic
T770 2102-2107 IN denotes after
T769 2096-2101 NN denotes brain
T768 2092-2095 DT denotes the
T767 2089-2091 IN denotes in
T766 2076-2088 NN denotes inflammation
T765 2072-2075 DT denotes the
T764 2062-2071 VB denotes regulates
T763 2057-2061 NN denotes Nrf2
T762 2052-2056 IN denotes that
T761 2039-2051 VB denotes demonstrated
T760 2034-2038 VB denotes have
T759 2026-2033 NN denotes studies
T758 2018-2025 JJ denotes Several
T757 2011-2016 NN denotes genes
T756 1999-2010 JJ denotes antioxidant
T755 1996-1998 IN denotes of
T754 1990-1995 NN denotes kinds
T753 1985-1989 JJ denotes many
T752 1975-1984 VB denotes regulates
T751 1970-1974 WDT denotes that
T750 1963-1969 NN denotes factor
T749 1949-1962 NN denotes transcription
T748 1947-1948 DT denotes a
T747 1944-1946 VB denotes is
T746 1942-1943 -RRB- denotes )
T745 1938-1942 NN denotes Nrf2
T744 1937-1938 -LRB- denotes (
T743 1929-1936 NN denotes factor2
T742 1919-1928 JJ denotes 2-related
T741 1909-1918 JJ denotes erythroid
T740 1902-1908 NN denotes factor
T739 1894-1901 JJ denotes Nuclear
T738 1891-1892 -RRB- denotes ]
T737 1888-1891 CD denotes 2–4
T736 1887-1888 -LRB- denotes [
T735 1880-1886 NN denotes organs
T734 1874-1879 JJ denotes other
T733 1871-1873 IN denotes in
T732 1866-1870 RB denotes also
T731 1862-1865 CC denotes but
T730 1856-1861 NN denotes brain
T729 1853-1855 IN denotes in
T728 1848-1852 RB denotes only
T727 1844-1847 RB denotes not
T726 1837-1843 NN denotes damage
T725 1827-1836 JJ denotes secondary
T724 1824-1826 TO denotes to
T723 1818-1823 VB denotes leads
T722 1805-1817 NN denotes inflammation
T721 1794-1804 JJ denotes subsequent
T720 1790-1793 DT denotes the
T719 1786-1789 CC denotes And
T718 1783-1784 -RRB- denotes ]
T717 1782-1783 CD denotes 1
T716 1781-1782 -LRB- denotes [
T715 1773-1780 NN denotes factors
T714 1761-1772 JJ denotes chemotactic
T713 1757-1760 CC denotes and
T712 1744-1756 NN denotes interleukins
T711 1741-1743 IN denotes as
T710 1736-1740 JJ denotes such
T709 1726-1735 NN denotes mediators
T708 1719-1725 JJ denotes immune
T707 1710-1718 JJ denotes numerous
T706 1707-1709 IN denotes of
T705 1699-1706 NN denotes release
T704 1695-1698 DT denotes the
T703 1692-1694 IN denotes as
T702 1687-1691 RB denotes well
T701 1684-1686 RB denotes as
T700 1682-1683 -COMMA- denotes ,
T699 1677-1682 NN denotes cells
T698 1661-1676 JJ denotes immunocompetent
T697 1652-1660 JJ denotes resident
T696 1649-1651 IN denotes of
T695 1638-1648 NN denotes activation
T694 1634-1637 CC denotes and
T693 1632-1633 -COMMA- denotes ,
T692 1627-1632 NN denotes cells
T691 1621-1626 NN denotes blood
T690 1610-1620 JJ denotes peripheral
T689 1607-1609 IN denotes of
T688 1594-1606 NN denotes infiltration
T687 1592-1593 -COMMA- denotes ,
T686 1587-1592 NN denotes edema
T685 1581-1586 NN denotes brain
T684 1579-1580 -COMMA- denotes ,
T683 1578-1579 -RRB- denotes )
T682 1575-1578 NN denotes BBB
T681 1574-1575 -LRB- denotes (
T680 1566-1573 NN denotes barrier
T679 1554-1565 NN denotes blood-brain
T678 1551-1553 IN denotes of
T677 1541-1550 NN denotes breakdown
T676 1531-1540 VB denotes including
T675 1529-1530 -COMMA- denotes ,
T674 1521-1529 NN denotes response
T673 1508-1520 JJ denotes inflammatory
T672 1502-1507 JJ denotes acute
T671 1499-1501 DT denotes an
T670 1496-1498 IN denotes of
T669 1485-1495 NN denotes initiation
T668 1481-1484 DT denotes the
T667 1472-1480 VB denotes involves
T666 1462-1471 NN denotes mechanism
T665 1455-1461 NN denotes injury
T664 1445-1454 JJ denotes secondary
T663 1441-1444 DT denotes The
T662 1429-1439 NN denotes mechanisms
T661 1427-1428 -RRB- denotes )
T660 1421-1427 NN denotes injury
T659 1413-1420 VB denotes delayed
T658 1410-1412 CC denotes or
T657 1400-1409 JJ denotes secondary
T656 1399-1400 -LRB- denotes (
T655 1390-1398 JJ denotes indirect
T654 1386-1389 CC denotes and
T653 1384-1385 -RRB- denotes )
T652 1378-1384 NN denotes injury
T651 1370-1377 JJ denotes primary
T650 1369-1370 -LRB- denotes (
T649 1362-1368 JJ denotes direct
T648 1359-1361 IN denotes of
T647 1352-1358 NN denotes result
T646 1350-1351 DT denotes a
T645 1347-1349 VB denotes is
T644 1340-1346 NN denotes injury
T643 1330-1339 JJ denotes traumatic
T642 1320-1329 VB denotes following
T641 1313-1319 NN denotes damage
T640 1307-1312 NN denotes Brain
T1293 3165-3168 IN denotes for
T1292 3160-3164 NN denotes mice
T1291 3158-3159 CD denotes 6
T1290 3157-3158 -LRB- denotes (
T1289 3152-3156 NN denotes mice
T1288 3150-3151 -RRB- denotes )
T1287 3148-3150 NN denotes KO
T1286 3147-3148 -LRB- denotes (
T1285 3138-3146 NN denotes knockout
T1284 3134-3137 CC denotes and
T1283 3132-3133 -RRB- denotes )
T1282 3130-3132 NN denotes WT
T1281 3129-3130 -LRB- denotes (
T1280 3124-3128 NN denotes type
T1279 3119-3123 JJ denotes wide
T1278 3114-3118 NN denotes Nrf2
T1277 3104-3113 JJ denotes 2-day-old
T1276 3094-3103 JJ denotes postnatal
T1275 3089-3093 IN denotes from
T1274 3080-3088 VB denotes obtained
T1273 3075-3079 VB denotes were
T1272 3064-3074 NN denotes astrocytes
T1271 3056-3063 JJ denotes Primary
T1270 3053-3054 -RRB- denotes )
T1269 3050-3053 NNP denotes USA
T1268 3048-3049 -COMMA- denotes ,
T1267 3046-3048 NNP denotes MD
T1266 3044-3045 -COMMA- denotes ,
T1265 3035-3044 NNP denotes Baltimore
T1264 3033-3034 -COMMA- denotes ,
T1263 3023-3033 NNP denotes University
T1262 3015-3022 NNP denotes Hopkins
T1261 3009-3014 NNP denotes Johns
T1260 3008-3009 -LRB- denotes (
T1259 3000-3007 NNP denotes Kensler
T1258 2997-2999 NNP denotes W.
T1257 2990-2996 NNP denotes Thomas
T1256 2986-2989 NNP denotes Dr.
T1255 2983-2985 IN denotes by
T1254 2974-2982 VB denotes provided
T1253 2967-2973 RB denotes kindly
T1252 2962-2966 VB denotes were
T1251 2957-2961 NN denotes mice
T1250 2953-2956 NN denotes ICR
T1249 2944-2952 NN denotes knockout
T897 2844-2850 NN denotes injury
T896 2836-2843 NN denotes scratch
T895 2830-2835 IN denotes after
T894 2825-2829 NN denotes mice
T893 2814-2824 JJ denotes transgenic
T892 2809-2813 IN denotes from
T891 2798-2808 NN denotes astrocytes
T890 2789-2797 VB denotes cultured
T889 2781-2788 JJ denotes primary
T888 2778-2780 IN denotes in
T887 2768-2777 NN denotes cytokines
T886 2752-2767 JJ denotes proinflammatory
T885 2749-2751 IN denotes of
T884 2738-2748 NN denotes expression
T883 2734-2737 DT denotes the
T882 2731-2733 IN denotes on
T881 2726-2730 NN denotes Nrf2
T880 2723-2725 IN denotes of
T879 2713-2722 NN denotes influence
T878 2709-2712 DT denotes the
T877 2700-2708 VB denotes analysed
T876 2697-2699 PRP denotes we
T875 2692-2696 RB denotes here
T874 2690-2691 -COMMA- denotes ,
T873 2681-2690 RB denotes Therefore
T872 2676-2679 NN denotes TBI
T871 2670-2675 IN denotes after
T870 2659-2669 NN denotes astrocytes
T869 2656-2658 IN denotes in
T868 2643-2655 NN denotes inflammation
T867 2639-2642 CC denotes and
T866 2634-2638 NN denotes Nrf2
T865 2626-2633 IN denotes between
T864 2613-2625 NN denotes relationship
T863 2609-2612 DT denotes the
T862 2603-2608 IN denotes about
T861 2597-2602 VB denotes known
T860 2594-2596 VB denotes is
T859 2587-2593 JJ denotes Little
T858 2581-2585 FW denotes vivo
T857 2578-2580 FW denotes in
T856 2568-2577 VB denotes performed
T855 2564-2567 VB denotes are
T854 2556-2563 NN denotes studies
T853 2541-2555 JJ denotes aforementioned
T852 2537-2540 DT denotes the
T851 2533-2536 PDT denotes all
T850 2531-2532 -COMMA- denotes ,
T849 2527-2531 NN denotes fact
T848 2524-2526 IN denotes In
T847 2515-2522 NN denotes disease
T846 2512-2514 CC denotes or
T845 2505-2511 NN denotes injury
T844 2502-2504 TO denotes to
T843 2491-2501 VB denotes responding
T842 2487-2490 CC denotes and
T841 2476-2486 NN denotes physiology
T840 2470-2475 NN denotes brain
T839 2463-2469 JJ denotes normal
T838 2451-2462 VB denotes maintaining
T837 2448-2450 IN denotes in
T836 2443-2447 NN denotes role
T835 2434-2442 JJ denotes critical
T834 2432-2433 DT denotes a
T833 2427-2431 VB denotes play
T832 2416-2426 NN denotes astrocytes
T831 2411-2415 IN denotes that
T830 2400-2410 VB denotes documented
T829 2395-2399 RB denotes well
T828 2392-2394 VB denotes is
T827 2389-2391 PRP denotes It
T826 2386-2387 -RRB- denotes ]
T825 2385-2386 CD denotes 6
T824 2384-2385 -LRB- denotes [
T823 2382-2383 -RRB- denotes )
T822 2379-2382 NN denotes SFN
T821 2378-2379 -LRB- denotes (
T820 2365-2377 NN denotes sulforaphane
T819 2362-2364 IN denotes as
T818 2357-2361 JJ denotes such
T817 2355-2356 -COMMA- denotes ,
T816 2347-2355 NN denotes inducers
T815 2343-2346 PRP-DOLLAR- denotes its
T814 2340-2342 IN denotes by
T813 2335-2339 NN denotes Nrf2
T812 2332-2334 IN denotes of
T811 2326-2331 NN denotes level
T810 2322-2325 DT denotes the
T809 2312-2321 VB denotes elevating
T808 2304-2311 IN denotes through
T807 2293-2303 VB denotes extenuated
T806 2290-2292 VB denotes be
T805 2286-2289 MD denotes can
T804 2273-2285 NN denotes inflammation
T803 2268-2272 JJ denotes such
T802 2266-2267 -COMMA- denotes ,
T801 2258-2266 NN denotes contrary
T800 2254-2257 DT denotes the
T799 2251-2253 IN denotes On
T798 2248-2249 -RRB- denotes ]
T797 2247-2248 CD denotes 5
T796 2246-2247 -LRB- denotes [
T795 2241-2245 NN denotes mice
T794 2232-2240 JJ denotes wildtype
T793 2229-2231 IN denotes in
T183 817-819 TO denotes to
T182 807-816 VB denotes performed
T181 803-806 VB denotes was
T180 792-802 NN denotes zymography
T179 784-791 NN denotes Gelatin
T178 773-782 VB denotes evaluated
T177 768-772 RB denotes also
T176 763-767 VB denotes were
T175 758-762 NN denotes MMP9
T174 754-757 CC denotes and
T173 752-753 -COMMA- denotes ,
T172 748-752 NN denotes IL-6
T171 746-747 -COMMA- denotes ,
T170 741-746 NN denotes IL-1β
T169 739-740 -COMMA- denotes ,
T168 734-739 NN denotes TNF-α
T167 731-733 IN denotes of
T166 724-730 NN denotes levels
T165 716-723 NN denotes protein
T164 712-715 CC denotes and
T163 707-711 NN denotes mRNA
T162 703-706 DT denotes The
T161 697-701 NN denotes EMSA
T160 694-696 IN denotes by
T159 684-693 VB denotes evaluated
T158 680-683 VB denotes was
T157 674-679 NN denotes NF-κB
T156 671-673 IN denotes of
T155 662-670 NN denotes activity
T154 650-661 JJ denotes DNA-binding
T153 646-649 DT denotes the
T152 641-645 RB denotes Then
T151 634-639 FW denotes vitro
T150 631-633 FW denotes in
T149 624-630 NN denotes injury
T148 614-623 JJ denotes traumatic
T147 610-613 DT denotes the
T146 604-609 VB denotes mimic
T145 601-603 TO denotes to
T144 591-600 VB denotes scratched
T143 586-590 VB denotes were
T142 575-585 NN denotes astrocytes
T141 566-574 VB denotes cultured
T140 558-565 JJ denotes Primary
T139 551-556 FW denotes vitro
T138 548-550 FW denotes in
T137 544-547 NN denotes TBI
T136 541-543 IN denotes of
T135 535-540 NN denotes model
T134 533-534 DT denotes a
T133 530-532 IN denotes in
T132 520-529 NN denotes cytokines
T131 504-519 JJ denotes proinflammatory
T130 501-503 IN denotes of
T129 490-500 NN denotes expression
T128 486-489 CC denotes and
T127 480-485 NN denotes NF-κB
T126 477-479 IN denotes of
T125 466-476 NN denotes activation
T124 462-465 DT denotes the
T123 459-461 IN denotes on
T122 454-458 NN denotes Nrf2
T121 451-453 IN denotes of
T120 441-450 NN denotes influence
T119 437-440 DT denotes the
T118 431-436 VB denotes study
T117 428-430 TO denotes to
T116 423-427 NN denotes mice
T115 414-422 JJ denotes knockout
T114 411-413 CC denotes or
T113 402-410 NN denotes wildtype
T112 397-401 NN denotes Nrf2
T111 390-396 CC denotes either
T110 385-389 IN denotes from
T109 374-384 NN denotes astrocytes
T108 365-373 VB denotes cultured
T107 357-364 JJ denotes primary
T106 352-356 VB denotes used
T105 349-351 PRP denotes we
T104 347-348 -COMMA- denotes ,
T103 342-347 RB denotes Hence
T102 337-340 NN denotes TBI
T101 331-336 IN denotes after
T100 320-330 NN denotes astrocytes
T99 317-319 IN denotes in
T98 311-316 NN denotes NF-κB
T97 307-310 CC denotes and
T96 302-306 NN denotes Nrf2
T95 294-301 IN denotes between
T94 281-293 NN denotes relationship
T93 277-280 DT denotes the
T92 271-276 IN denotes about
T91 265-270 VB denotes known
T90 262-264 VB denotes is
T89 255-261 JJ denotes little
T88 251-254 CC denotes but
T87 249-250 -COMMA- denotes ,
T86 246-249 NN denotes TBI
T85 240-245 IN denotes after
T84 234-239 NN denotes brain
T83 230-233 DT denotes the
T82 227-229 IN denotes in
T81 221-226 NN denotes NF-κB
T80 218-220 IN denotes of
T79 207-217 NN denotes activation
T78 199-206 IN denotes through
T77 191-198 NN denotes process
T76 178-190 JJ denotes inflammatory
T75 169-177 VB denotes enhances
T74 159-168 NN denotes depletion
T73 154-158 NN denotes Nrf2
T72 149-153 IN denotes that
T71 142-148 VB denotes proved
T70 137-141 VB denotes been
T69 133-136 VB denotes has
T68 130-132 PRP denotes It
T254 1278-1288 NN denotes astrocytes
T253 1275-1277 IN denotes in
T252 1265-1274 NN denotes cytokines
T251 1249-1264 JJ denotes proinflammatory
T250 1238-1248 JJ denotes downstream
T249 1234-1237 CC denotes and
T248 1228-1233 NN denotes NF-κB
T247 1225-1227 IN denotes of
T246 1214-1224 NN denotes activation
T245 1206-1213 IN denotes through
T244 1193-1205 NN denotes inflammation
T243 1182-1192 JJ denotes aggressive
T242 1177-1181 RB denotes more
T241 1170-1176 VB denotes induce
T240 1166-1169 MD denotes may
T239 1161-1165 NN denotes Nrf2
T238 1158-1160 IN denotes of
T237 1150-1157 NN denotes absence
T236 1146-1149 DT denotes the
T235 1141-1145 IN denotes that
T234 1133-1140 VB denotes suggest
T233 1125-1132 NN denotes results
T232 1119-1124 DT denotes These
T231 1107-1117 NN denotes astrocytes
T230 1104-1106 JJ denotes WT
T229 1101-1103 IN denotes in
T228 1096-1100 DT denotes that
T227 1091-1095 IN denotes than
T226 1080-1090 NN denotes astrocytes
T225 1077-1079 NN denotes KO
T224 1072-1076 NN denotes Nrf2
T223 1069-1071 IN denotes in
T222 1059-1068 JJ denotes prominent
T221 1054-1058 RB denotes more
T220 1049-1053 RB denotes much
T219 1045-1048 VB denotes was
T218 1036-1044 NN denotes tendency
T217 1031-1035 JJ denotes such
T216 1027-1030 CC denotes And
T215 1017-1025 VB denotes elevated
T214 1012-1016 RB denotes also
T213 1007-1011 VB denotes were
T212 1002-1006 NN denotes MMP9
T211 999-1001 IN denotes of
T210 990-998 NN denotes activity
T209 986-989 CC denotes and
T208 975-985 NN denotes expression
T207 971-974 DT denotes The
T206 962-969 NN denotes scratch
T205 956-961 IN denotes after
T204 954-955 NN denotes h
T203 951-953 CD denotes 24
T202 948-950 IN denotes at
T201 936-947 VB denotes upregulated
T200 931-935 VB denotes were
T199 925-930 IN denotes above
T198 915-924 VB denotes mentioned
T197 905-914 NN denotes cytokines
T196 889-904 JJ denotes proinflammatory
T195 886-888 IN denotes of
T194 875-885 NN denotes expression
T193 871-874 CC denotes and
T192 865-870 NN denotes NF-κB
T191 862-864 IN denotes of
T190 853-861 NN denotes activity
T189 849-852 DT denotes The
T188 843-847 NN denotes MMP9
T187 840-842 IN denotes of
T186 831-839 NN denotes activity
T185 827-830 DT denotes the
T184 820-826 VB denotes detect
T4100 9493-9502 VB denotes collected
T4099 9489-9492 VB denotes was
T4098 9477-9488 NN denotes supernatant
T4097 9473-9476 DT denotes The
T4096 9466-9471 IN denotes above
T4095 9456-9465 VB denotes mentioned
T4094 9453-9455 IN denotes as
T4093 9441-9452 VB denotes homogenized
T4092 9436-9440 VB denotes were
T4091 9429-9435 NN denotes groups
T4090 9424-9428 CD denotes four
T4089 9421-9423 IN denotes of
T4088 9415-9420 NN denotes Cells
T4087 9413-9414 -RRB- denotes )
T4086 9408-9413 NN denotes ELISA
T4085 9407-9408 -LRB- denotes (
T4084 9401-9406 NN denotes Assay
T4083 9387-9400 JJ denotes Immunosorbent
T4082 9373-9386 JJ denotes Enzyme-Linked
T4081 9368-9371 CD denotes 2.7
T6605 15039-15043 CD denotes 1.01
T6604 15032-15038 IN denotes versus
T6603 15026-15031 CD denotes 0.090
R1 T54 T53 arg1Of Absence,The
R2 T54 T55 arg1Of Absence,of
R3 T54 T57 arg1Of Absence,Enhances
R4 T56 T55 arg2Of Nrf2,of
R5 T57 T60 arg1Of Enhances,following
R6 T59 T57 arg2Of Inflammation,Enhances
R7 T59 T58 arg1Of Inflammation,NF-κB-Dependent
R8 T62 T60 arg2Of Injury,following
R9 T62 T61 arg1Of Injury,Scratch
R10 T62 T63 arg1Of Injury,in
R11 T66 T64 arg1Of Cultured,Mouse
R12 T66 T65 arg1Of Cultured,Primary
R13 T67 T63 arg2Of Astrocytes,in
R14 T67 T66 arg1Of Astrocytes,Cultured
R15 T71 T69 arg2Of proved,has
R16 T71 T70 arg2Of proved,been
R17 T74 T73 arg1Of depletion,Nrf2
R18 T74 T75 arg1Of depletion,enhances
R19 T75 T78 arg1Of enhances,through
R20 T75 T88 arg1Of enhances,but
R21 T77 T75 arg2Of process,enhances
R22 T77 T76 arg1Of process,inflammatory
R23 T79 T78 arg2Of activation,through
R24 T79 T80 arg1Of activation,of
R25 T79 T82 arg1Of activation,in
R26 T81 T80 arg2Of NF-κB,of
R27 T84 T82 arg2Of brain,in
R28 T84 T83 arg1Of brain,the
R29 T84 T85 arg1Of brain,after
R30 T86 T85 arg2Of TBI,after
R31 T88 T68 arg1Of but,It
R32 T88 T69 arg1Of but,has
R33 T88 T70 arg1Of but,been
R34 T88 T71 arg2Of but,proved
R35 T88 T72 arg1Of but,that
R36 T88 T87 arg1Of but,","
R37 T89 T90 arg1Of little,is
R38 T89 T91 arg2Of little,known
R39 T91 T88 arg2Of known,but
R40 T91 T90 arg2Of known,is
R41 T91 T92 arg1Of known,about
R42 T94 T92 arg2Of relationship,about
R43 T94 T93 arg1Of relationship,the
R44 T94 T95 arg1Of relationship,between
R45 T96 T97 arg1Of Nrf2,and
R46 T97 T95 arg2Of and,between
R47 T97 T99 arg1Of and,in
R48 T97 T101 arg1Of and,after
R49 T98 T97 arg2Of NF-κB,and
R50 T100 T99 arg2Of astrocytes,in
R51 T102 T101 arg2Of TBI,after
R52 T105 T106 arg1Of we,used
R53 T106 T103 arg1Of used,Hence
R54 T106 T104 arg1Of used,","
R55 T109 T106 arg2Of astrocytes,used
R56 T109 T107 arg1Of astrocytes,primary
R57 T109 T108 arg2Of astrocytes,cultured
R58 T109 T110 arg1Of astrocytes,from
R59 T109 T117 modOf astrocytes,to
R60 T109 T118 arg1Of astrocytes,study
R61 T113 T112 arg1Of wildtype,Nrf2
R62 T113 T114 arg1Of wildtype,or
R63 T114 T110 arg2Of or,from
R64 T114 T111 arg1Of or,either
R65 T116 T114 arg2Of mice,or
R66 T116 T115 arg1Of mice,knockout
R67 T118 T117 arg1Of study,to
R68 T118 T133 arg1Of study,in
R69 T120 T118 arg2Of influence,study
R70 T120 T119 arg1Of influence,the
R71 T120 T121 arg1Of influence,of
R72 T120 T123 arg1Of influence,on
R73 T122 T121 arg2Of Nrf2,of
R74 T125 T124 arg1Of activation,the
R75 T125 T126 arg1Of activation,of
R76 T125 T128 arg1Of activation,and
R77 T127 T126 arg2Of NF-κB,of
R78 T128 T123 arg2Of and,on
R79 T129 T128 arg2Of expression,and
R80 T129 T130 arg1Of expression,of
R81 T132 T130 arg2Of cytokines,of
R82 T132 T131 arg1Of cytokines,proinflammatory
R83 T135 T133 arg2Of model,in
R84 T135 T134 arg1Of model,a
R85 T135 T136 arg1Of model,of
R86 T135 T139 arg1Of model,vitro
R87 T137 T136 arg2Of TBI,of
R88 T139 T138 arg1Of vitro,in
R89 T142 T140 arg1Of astrocytes,Primary
R90 T142 T141 arg2Of astrocytes,cultured
R91 T142 T143 arg1Of astrocytes,were
R92 T142 T144 arg2Of astrocytes,scratched
R93 T142 T146 arg1Of astrocytes,mimic
R94 T144 T143 arg2Of scratched,were
R95 T146 T144 arg3Of mimic,scratched
R96 T146 T145 arg1Of mimic,to
R97 T146 T151 arg1Of mimic,vitro
R98 T149 T146 arg2Of injury,mimic
R99 T149 T147 arg1Of injury,the
R100 T149 T148 arg1Of injury,traumatic
R101 T151 T150 arg1Of vitro,in
R102 T155 T153 arg1Of activity,the
R103 T155 T154 arg1Of activity,DNA-binding
R104 T155 T156 arg1Of activity,of
R105 T155 T158 arg1Of activity,was
R106 T155 T159 arg2Of activity,evaluated
R107 T157 T156 arg2Of NF-κB,of
R108 T159 T152 arg1Of evaluated,Then
R109 T159 T158 arg2Of evaluated,was
R110 T161 T159 arg1Of EMSA,evaluated
R111 T161 T160 arg2Of EMSA,by
R112 T163 T164 arg1Of mRNA,and
R113 T165 T164 arg2Of protein,and
R114 T166 T162 arg1Of levels,The
R115 T166 T163 arg1Of levels,mRNA
R116 T166 T165 arg1Of levels,protein
R117 T166 T167 arg1Of levels,of
R118 T166 T176 arg1Of levels,were
R119 T166 T178 arg2Of levels,evaluated
R120 T168 T169 arg1Of TNF-α,","
R121 T169 T171 arg1Of ",",","
R122 T170 T169 arg2Of IL-1β,","
R123 T171 T174 arg1Of ",",and
R124 T172 T171 arg2Of IL-6,","
R125 T174 T167 arg2Of and,of
R126 T174 T173 arg1Of and,","
R127 T175 T174 arg2Of MMP9,and
R128 T178 T176 arg2Of evaluated,were
R129 T178 T177 arg1Of evaluated,also
R130 T180 T179 arg1Of zymography,Gelatin
R131 T180 T181 arg1Of zymography,was
R132 T180 T182 arg2Of zymography,performed
R133 T182 T181 arg2Of performed,was
R134 T184 T182 arg3Of detect,performed
R135 T184 T183 arg1Of detect,to
R136 T186 T184 arg2Of activity,detect
R137 T186 T185 arg1Of activity,the
R138 T186 T187 arg1Of activity,of
R139 T188 T187 arg2Of MMP9,of
R140 T190 T189 arg1Of activity,The
R141 T190 T191 arg1Of activity,of
R142 T190 T200 arg1Of activity,were
R143 T190 T201 arg2Of activity,upregulated
R144 T192 T193 arg1Of NF-κB,and
R145 T193 T191 arg2Of and,of
R146 T193 T195 arg1Of and,of
R147 T194 T193 arg2Of expression,and
R148 T197 T195 arg2Of cytokines,of
R149 T197 T196 arg1Of cytokines,proinflammatory
R150 T197 T198 arg2Of cytokines,mentioned
R151 T198 T199 arg1Of mentioned,above
R152 T201 T200 arg2Of upregulated,were
R153 T201 T202 arg1Of upregulated,at
R154 T204 T202 arg2Of h,at
R155 T204 T203 arg1Of h,24
R156 T204 T205 arg1Of h,after
R157 T206 T205 arg2Of scratch,after
R158 T208 T209 arg1Of expression,and
R159 T209 T207 arg1Of and,The
R160 T209 T211 arg1Of and,of
R161 T209 T213 arg1Of and,were
R162 T209 T215 arg2Of and,elevated
R163 T210 T209 arg2Of activity,and
R164 T212 T211 arg2Of MMP9,of
R165 T215 T213 arg2Of elevated,were
R166 T215 T214 arg1Of elevated,also
R167 T218 T217 arg1Of tendency,such
R168 T218 T219 arg1Of tendency,was
R169 T218 T222 arg1Of tendency,prominent
R170 T219 T216 arg1Of was,And
R171 T221 T220 arg1Of more,much
R172 T222 T219 arg2Of prominent,was
R173 T222 T221 arg1Of prominent,more
R174 T222 T223 arg1Of prominent,in
R175 T222 T227 arg1Of prominent,than
R176 T226 T223 arg2Of astrocytes,in
R177 T226 T224 arg1Of astrocytes,Nrf2
R178 T226 T225 arg1Of astrocytes,KO
R179 T228 T227 arg2Of that,than
R180 T228 T229 arg1Of that,in
R181 T231 T229 arg2Of astrocytes,in
R182 T231 T230 arg1Of astrocytes,WT
R183 T233 T232 arg1Of results,These
R184 T233 T234 arg1Of results,suggest
R185 T237 T236 arg1Of absence,the
R186 T237 T238 arg1Of absence,of
R187 T237 T240 arg1Of absence,may
R188 T237 T241 arg1Of absence,induce
R189 T239 T238 arg2Of Nrf2,of
R190 T241 T234 arg2Of induce,suggest
R191 T241 T235 arg1Of induce,that
R192 T241 T240 arg2Of induce,may
R193 T241 T245 arg1Of induce,through
R194 T243 T242 arg1Of aggressive,more
R195 T244 T241 arg2Of inflammation,induce
R196 T244 T243 arg1Of inflammation,aggressive
R197 T246 T245 arg2Of activation,through
R198 T246 T247 arg1Of activation,of
R199 T246 T253 arg1Of activation,in
R200 T248 T249 arg1Of NF-κB,and
R201 T249 T247 arg2Of and,of
R202 T252 T249 arg2Of cytokines,and
R203 T252 T250 arg1Of cytokines,downstream
R204 T252 T251 arg1Of cytokines,proinflammatory
R205 T254 T253 arg2Of astrocytes,in
R451 T641 T640 arg1Of damage,Brain
R452 T641 T642 arg1Of damage,following
R453 T641 T645 arg1Of damage,is
R454 T644 T642 arg2Of injury,following
R455 T644 T643 arg1Of injury,traumatic
R456 T647 T645 arg2Of result,is
R457 T647 T646 arg1Of result,a
R458 T647 T648 arg1Of result,of
R459 T649 T650 arg1Of direct,(
R460 T649 T654 arg1Of direct,and
R461 T652 T650 arg2Of injury,(
R462 T652 T651 arg1Of injury,primary
R463 T653 T650 arg3Of ),(
R464 T655 T654 arg2Of indirect,and
R465 T655 T656 arg1Of indirect,(
R466 T660 T656 arg2Of injury,(
R467 T660 T657 arg1Of injury,secondary
R468 T660 T658 arg1Of injury,or
R469 T660 T659 arg2Of injury,delayed
R470 T661 T656 arg3Of ),(
R471 T662 T648 arg2Of mechanisms,of
R472 T662 T649 arg1Of mechanisms,direct
R473 T662 T655 arg1Of mechanisms,indirect
R474 T666 T663 arg1Of mechanism,The
R475 T666 T664 arg1Of mechanism,secondary
R476 T666 T665 arg1Of mechanism,injury
R477 T666 T667 arg1Of mechanism,involves
R478 T669 T667 arg2Of initiation,involves
R479 T669 T668 arg1Of initiation,the
R480 T669 T670 arg1Of initiation,of
R481 T669 T675 arg1Of initiation,","
R482 T669 T676 arg1Of initiation,including
R483 T674 T670 arg2Of response,of
R484 T674 T671 arg1Of response,an
R485 T674 T672 arg1Of response,acute
R486 T674 T673 arg1Of response,inflammatory
R487 T677 T676 arg2Of breakdown,including
R488 T677 T678 arg1Of breakdown,of
R489 T680 T679 arg1Of barrier,blood-brain
R490 T680 T681 arg1Of barrier,(
R491 T680 T684 arg1Of barrier,","
R492 T682 T681 arg2Of BBB,(
R493 T683 T681 arg3Of ),(
R494 T684 T687 arg1Of ",",","
R495 T686 T684 arg2Of edema,","
R496 T686 T685 arg1Of edema,brain
R497 T687 T694 arg1Of ",",and
R498 T688 T687 arg2Of infiltration,","
R499 T688 T689 arg1Of infiltration,of
R500 T692 T689 arg2Of cells,of
R501 T692 T690 arg1Of cells,peripheral
R502 T692 T691 arg1Of cells,blood
R503 T694 T693 arg1Of and,","
R504 T694 T703 arg1Of and,as
R505 T695 T694 arg2Of activation,and
R506 T695 T696 arg1Of activation,of
R507 T699 T696 arg2Of cells,of
R508 T699 T697 arg1Of cells,resident
R509 T699 T698 arg1Of cells,immunocompetent
R510 T703 T678 arg2Of as,of
R511 T703 T700 arg1Of as,","
R512 T703 T701 arg1Of as,as
R513 T703 T702 arg1Of as,well
R514 T705 T703 arg2Of release,as
R515 T705 T704 arg1Of release,the
R516 T705 T706 arg1Of release,of
R517 T709 T706 arg2Of mediators,of
R518 T709 T707 arg1Of mediators,numerous
R519 T709 T708 arg1Of mediators,immune
R520 T709 T711 arg1Of mediators,as
R521 T711 T710 arg1Of as,such
R522 T712 T713 arg1Of interleukins,and
R523 T713 T711 arg2Of and,as
R524 T715 T713 arg2Of factors,and
R525 T715 T714 arg1Of factors,chemotactic
R526 T715 T716 arg1Of factors,[
R527 T717 T716 arg2Of 1,[
R528 T718 T716 arg3Of ],[
R529 T722 T720 arg1Of inflammation,the
R530 T722 T721 arg1Of inflammation,subsequent
R531 T722 T723 arg1Of inflammation,leads
R532 T723 T719 arg1Of leads,And
R533 T723 T724 arg1Of leads,to
R534 T723 T729 arg1Of leads,in
R535 T723 T733 arg1Of leads,in
R536 T726 T724 arg2Of damage,to
R537 T726 T725 arg1Of damage,secondary
R538 T728 T727 arg1Of only,not
R539 T729 T731 arg1Of in,but
R540 T730 T729 arg2Of brain,in
R541 T731 T728 arg1Of but,only
R542 T731 T732 arg1Of but,also
R543 T733 T731 arg2Of in,but
R544 T735 T729 arg3Of organs,in
R545 T735 T733 arg2Of organs,in
R546 T735 T734 arg1Of organs,other
R547 T735 T736 arg1Of organs,[
R548 T737 T736 arg2Of 2–4,[
R549 T738 T736 arg3Of ],[
R550 T741 T739 arg1Of erythroid,Nuclear
R551 T741 T740 arg1Of erythroid,factor
R552 T743 T741 arg1Of factor2,erythroid
R553 T743 T742 arg1Of factor2,2-related
R554 T743 T744 arg1Of factor2,(
R555 T743 T747 arg1Of factor2,is
R556 T745 T744 arg2Of Nrf2,(
R557 T746 T744 arg3Of ),(
R558 T750 T747 arg2Of factor,is
R559 T750 T748 arg1Of factor,a
R560 T750 T749 arg1Of factor,transcription
R561 T750 T751 arg1Of factor,that
R562 T750 T752 arg1Of factor,regulates
R563 T754 T752 arg2Of kinds,regulates
R564 T754 T753 arg1Of kinds,many
R565 T754 T755 arg1Of kinds,of
R566 T757 T755 arg2Of genes,of
R567 T757 T756 arg1Of genes,antioxidant
R568 T759 T758 arg1Of studies,Several
R569 T759 T760 arg1Of studies,have
R570 T759 T761 arg1Of studies,demonstrated
R571 T761 T760 arg2Of demonstrated,have
R572 T763 T764 arg1Of Nrf2,regulates
R573 T764 T761 arg2Of regulates,demonstrated
R574 T764 T762 arg1Of regulates,that
R575 T764 T767 arg1Of regulates,in
R576 T764 T770 arg1Of regulates,after
R577 T766 T764 arg2Of inflammation,regulates
R578 T766 T765 arg1Of inflammation,the
R579 T769 T767 arg2Of brain,in
R580 T769 T768 arg1Of brain,the
R581 T773 T770 arg2Of injury,after
R582 T773 T771 arg1Of injury,traumatic
R583 T773 T772 arg1Of injury,brain
R584 T773 T774 arg1Of injury,(
R585 T775 T774 arg2Of TBI,(
R586 T776 T774 arg3Of ),(
R587 T780 T779 arg1Of showed,also
R588 T782 T783 arg1Of TBI,could
R589 T782 T784 arg1Of TBI,induce
R590 T784 T780 arg2Of induce,showed
R591 T784 T781 arg1Of induce,that
R592 T784 T783 arg2Of induce,could
R593 T784 T792 arg1Of induce,than
R594 T784 T796 arg1Of induce,[
R595 T786 T785 arg1Of aggravated,more
R596 T787 T784 arg2Of damage,induce
R597 T787 T786 arg1Of damage,aggravated
R598 T787 T788 arg1Of damage,in
R599 T791 T788 arg2Of mice,in
R600 T791 T789 arg1Of mice,Nrf2
R601 T791 T790 arg1Of mice,knockout
R602 T793 T792 arg2Of in,than
R603 T795 T793 arg2Of mice,in
R604 T795 T794 arg1Of mice,wildtype
R605 T797 T796 arg2Of 5,[
R606 T798 T796 arg3Of ],[
R607 T801 T799 arg2Of contrary,On
R608 T801 T800 arg1Of contrary,the
R609 T804 T803 arg1Of inflammation,such
R610 T804 T805 arg1Of inflammation,can
R611 T804 T806 arg1Of inflammation,be
R612 T804 T807 arg2Of inflammation,extenuated
R613 T807 T799 arg1Of extenuated,On
R614 T807 T802 arg1Of extenuated,","
R615 T807 T805 arg2Of extenuated,can
R616 T807 T806 arg2Of extenuated,be
R617 T807 T808 arg1Of extenuated,through
R618 T809 T808 arg2Of elevating,through
R619 T811 T809 arg2Of level,elevating
R620 T811 T810 arg1Of level,the
R621 T811 T812 arg1Of level,of
R622 T811 T814 arg1Of level,by
R623 T811 T817 arg1Of level,","
R624 T811 T819 arg1Of level,as
R625 T813 T812 arg2Of Nrf2,of
R626 T816 T814 arg2Of inducers,by
R627 T816 T815 arg1Of inducers,its
R628 T819 T818 arg1Of as,such
R629 T820 T819 arg2Of sulforaphane,as
R630 T820 T821 arg1Of sulforaphane,(
R631 T820 T824 arg1Of sulforaphane,[
R632 T822 T821 arg2Of SFN,(
R633 T823 T821 arg3Of ),(
R634 T825 T824 arg2Of 6,[
R635 T826 T824 arg3Of ],[
R636 T827 T828 arg1Of It,is
R637 T827 T830 arg2Of It,documented
R638 T830 T828 arg2Of documented,is
R639 T830 T829 arg1Of documented,well
R640 T832 T833 arg1Of astrocytes,play
R641 T833 T830 arg3Of play,documented
R642 T833 T831 arg1Of play,that
R643 T833 T837 arg1Of play,in
R644 T836 T833 arg2Of role,play
R645 T836 T834 arg1Of role,a
R646 T836 T835 arg1Of role,critical
R647 T838 T842 arg1Of maintaining,and
R648 T841 T838 arg2Of physiology,maintaining
R649 T841 T839 arg1Of physiology,normal
R650 T841 T840 arg1Of physiology,brain
R651 T842 T837 arg2Of and,in
R652 T843 T842 arg2Of responding,and
R653 T843 T844 arg1Of responding,to
R654 T845 T846 arg1Of injury,or
R655 T846 T844 arg2Of or,to
R656 T847 T846 arg2Of disease,or
R657 T849 T848 arg2Of fact,In
R658 T854 T851 arg1Of studies,all
R659 T854 T852 arg1Of studies,the
R660 T854 T853 arg1Of studies,aforementioned
R661 T854 T855 arg1Of studies,are
R662 T854 T856 arg2Of studies,performed
R663 T856 T848 arg1Of performed,In
R664 T856 T850 arg1Of performed,","
R665 T856 T855 arg2Of performed,are
R666 T856 T858 arg1Of performed,vivo
R667 T858 T857 arg1Of vivo,in
R668 T859 T860 arg1Of Little,is
R669 T859 T861 arg2Of Little,known
R670 T861 T860 arg2Of known,is
R671 T861 T862 arg1Of known,about
R672 T864 T862 arg2Of relationship,about
R673 T864 T863 arg1Of relationship,the
R674 T864 T865 arg1Of relationship,between
R675 T866 T867 arg1Of Nrf2,and
R676 T867 T865 arg2Of and,between
R677 T867 T869 arg1Of and,in
R678 T867 T871 arg1Of and,after
R679 T868 T867 arg2Of inflammation,and
R680 T870 T869 arg2Of astrocytes,in
R681 T872 T871 arg2Of TBI,after
R682 T876 T877 arg1Of we,analysed
R683 T877 T873 arg1Of analysed,Therefore
R684 T877 T874 arg1Of analysed,","
R685 T877 T875 arg1Of analysed,here
R686 T879 T877 arg2Of influence,analysed
R687 T879 T878 arg1Of influence,the
R688 T879 T880 arg1Of influence,of
R689 T879 T882 arg1Of influence,on
R690 T881 T880 arg2Of Nrf2,of
R691 T884 T882 arg2Of expression,on
R692 T884 T883 arg1Of expression,the
R693 T884 T885 arg1Of expression,of
R694 T884 T888 arg1Of expression,in
R695 T887 T885 arg2Of cytokines,of
R696 T887 T886 arg1Of cytokines,proinflammatory
R697 T891 T888 arg2Of astrocytes,in
R698 T891 T889 arg1Of astrocytes,primary
R699 T891 T890 arg2Of astrocytes,cultured
R700 T891 T892 arg1Of astrocytes,from
R701 T894 T892 arg2Of mice,from
R702 T894 T893 arg1Of mice,transgenic
R703 T894 T895 arg1Of mice,after
R704 T897 T895 arg2Of injury,after
R705 T897 T896 arg1Of injury,scratch
R985 T1242 T1243 arg1Of Culture,and
R986 T1243 T1241 arg1Of and,Primary
R987 T1243 T1245 arg1Of and,of
R988 T1244 T1243 arg2Of Identification,and
R989 T1247 T1245 arg2Of Astrocytes,of
R990 T1247 T1246 arg1Of Astrocytes,Mouse
R991 T1251 T1248 arg1Of mice,Nrf2
R992 T1251 T1249 arg1Of mice,knockout
R993 T1251 T1250 arg1Of mice,ICR
R994 T1251 T1252 arg1Of mice,were
R995 T1251 T1254 arg2Of mice,provided
R996 T1254 T1252 arg2Of provided,were
R997 T1254 T1253 arg1Of provided,kindly
R998 T1259 T1254 arg1Of Kensler,provided
R999 T1259 T1255 arg2Of Kensler,by
R1000 T1259 T1256 arg1Of Kensler,Dr.
R1001 T1259 T1257 arg1Of Kensler,Thomas
R1002 T1259 T1258 arg1Of Kensler,W.
R1003 T1259 T1260 arg1Of Kensler,(
R1004 T1263 T1260 arg2Of University,(
R1005 T1263 T1261 arg1Of University,Johns
R1006 T1263 T1262 arg1Of University,Hopkins
R1007 T1263 T1264 arg1Of University,","
R1008 T1263 T1268 arg1Of University,","
R1009 T1263 T1269 arg1Of University,USA
R1010 T1265 T1264 arg2Of Baltimore,","
R1011 T1265 T1266 arg1Of Baltimore,","
R1012 T1267 T1266 arg2Of MD,","
R1013 T1270 T1260 arg3Of ),(
R1014 T1272 T1271 arg1Of astrocytes,Primary
R1015 T1272 T1273 arg1Of astrocytes,were
R1016 T1272 T1274 arg2Of astrocytes,obtained
R1017 T1274 T1273 arg2Of obtained,were
R1018 T1274 T1275 arg1Of obtained,from
R1019 T1280 T1276 arg1Of type,postnatal
R1020 T1280 T1277 arg1Of type,2-day-old
R1021 T1280 T1278 arg1Of type,Nrf2
R1022 T1280 T1279 arg1Of type,wide
R1023 T1280 T1281 arg1Of type,(
R1024 T1280 T1284 arg1Of type,and
R1025 T1282 T1281 arg2Of WT,(
R1026 T1283 T1281 arg3Of ),(
R1027 T1284 T1275 arg2Of and,from
R1028 T1287 T1286 arg2Of KO,(
R1029 T1288 T1286 arg3Of ),(
R1030 T1289 T1284 arg2Of mice,and
R1031 T1289 T1285 arg1Of mice,knockout
R1032 T1289 T1286 arg1Of mice,(
R1033 T1289 T1290 arg1Of mice,(
R1034 T1292 T1290 arg2Of mice,(
R1035 T1292 T1291 arg1Of mice,6
R1036 T1292 T1293 arg1Of mice,for
R1037 T1295 T1293 arg2Of genotype,for
R1038 T1295 T1294 arg1Of genotype,each
R1039 T1296 T1290 arg3Of ),(
R1040 T1298 T1297 arg2Of decapitation,Following
R1041 T1301 T1300 arg1Of cortices,the
R1042 T1301 T1302 arg1Of cortices,were
R1043 T1301 T1303 arg2Of cortices,dissected
R1044 T1303 T1302 arg2Of dissected,were
R1045 T1303 T1304 arg1Of dissected,out
R1046 T1303 T1306 arg1Of dissected,and
R1047 T1306 T1297 arg1Of and,Following
R1048 T1306 T1299 arg1Of and,","
R1049 T1306 T1305 arg1Of and,","
R1050 T1308 T1309 arg1Of meninges,and
R1051 T1309 T1307 arg1Of and,the
R1052 T1309 T1313 arg1Of and,were
R1053 T1309 T1314 arg2Of and,removed
R1054 T1312 T1309 arg2Of vessels,and
R1055 T1312 T1310 arg2Of vessels,associated
R1056 T1312 T1311 arg1Of vessels,blood
R1057 T1314 T1306 arg2Of removed,and
R1058 T1314 T1313 arg2Of removed,were
R1059 T1316 T1315 arg1Of tissue,The
R1060 T1316 T1317 arg1Of tissue,was
R1061 T1316 T1319 arg2Of tissue,chopped
R1062 T1316 T1337 arg1Of tissue,agitated
R1063 T1319 T1317 arg2Of chopped,was
R1064 T1319 T1318 arg1Of chopped,roughly
R1065 T1319 T1320 arg1Of chopped,with
R1066 T1319 T1336 arg1Of chopped,and
R1067 T1323 T1320 arg2Of blade,with
R1068 T1323 T1321 arg1Of blade,a
R1069 T1323 T1322 arg1Of blade,scalpel
R1070 T1323 T1324 arg1Of blade,","
R1071 T1323 T1325 arg2Of blade,incubated
R1072 T1325 T1326 arg1Of incubated,in
R1073 T1327 T1328 arg1Of 0.5,%
R1074 T1329 T1326 arg2Of trypsin,in
R1075 T1329 T1327 arg1Of trypsin,0.5
R1076 T1329 T1330 arg1Of trypsin,for
R1077 T1329 T1333 arg1Of trypsin,at
R1078 T1332 T1330 arg2Of minutes,for
R1079 T1332 T1331 arg1Of minutes,10
R1080 T1334 T1333 arg2Of 37°C,at
R1081 T1336 T1335 arg1Of and,","
R1082 T1337 T1336 arg2Of agitated,and
R1083 T1340 T1337 arg2Of minutes,agitated
R1084 T1340 T1338 arg1Of minutes,every
R1085 T1340 T1339 arg1Of minutes,few
R1086 T1342 T1341 arg2Of digestion,After
R1087 T1345 T1344 arg1Of tissue,the
R1088 T1345 T1346 arg1Of tissue,was
R1089 T1345 T1347 arg2Of tissue,rinsed
R1090 T1347 T1341 arg1Of rinsed,After
R1091 T1347 T1343 arg1Of rinsed,","
R1092 T1347 T1346 arg2Of rinsed,was
R1093 T1347 T1348 arg1Of rinsed,twice
R1094 T1347 T1349 arg1Of rinsed,in
R1095 T1350 T1349 arg2Of DMEM,in
R1096 T1350 T1351 arg1Of DMEM,with
R1097 T1352 T1353 arg1Of 10,%
R1098 T1354 T1352 arg1Of FBS,10
R1099 T1354 T1355 arg1Of FBS,","
R1100 T1354 T1356 arg2Of FBS,followed
R1101 T1354 T1371 arg1Of FBS,","
R1102 T1360 T1356 arg1Of dissociation,followed
R1103 T1360 T1357 arg2Of dissociation,by
R1104 T1360 T1358 arg1Of dissociation,a
R1105 T1360 T1359 arg1Of dissociation,mechanical
R1106 T1360 T1361 arg1Of dissociation,in
R1107 T1362 T1361 arg2Of DMEM,in
R1108 T1362 T1363 arg1Of DMEM,with
R1109 T1366 T1364 arg1Of FBS,20
R1110 T1366 T1365 arg1Of FBS,%
R1111 T1366 T1367 arg1Of FBS,and
R1112 T1367 T1363 arg2Of and,with
R1113 T1370 T1367 arg2Of penicillin,and
R1114 T1370 T1368 arg1Of penicillin,5
R1115 T1370 T1369 arg1Of penicillin,units/mL
R1116 T1371 T1351 arg2Of ",",with
R1117 T1374 T1371 arg2Of streptomycin,","
R1118 T1374 T1372 arg1Of streptomycin,5
R1119 T1374 T1373 arg1Of streptomycin,μg/mL
R1120 T1374 T1375 arg1Of streptomycin,(
R1121 T1378 T1375 arg2Of medium,(
R1122 T1378 T1376 arg1Of medium,complete
R1123 T1378 T1377 arg1Of medium,culture
R1124 T1379 T1375 arg3Of ),(
R1125 T1381 T1380 arg2Of incubation,After
R1126 T1381 T1382 arg1Of incubation,for
R1127 T1384 T1382 arg2Of h,for
R1128 T1384 T1383 arg1Of h,1
R1129 T1387 T1386 arg1Of supernatant,the
R1130 T1387 T1388 arg1Of supernatant,was
R1131 T1387 T1389 arg2Of supernatant,transferred
R1132 T1389 T1380 arg1Of transferred,After
R1133 T1389 T1385 arg1Of transferred,","
R1134 T1389 T1388 arg2Of transferred,was
R1135 T1389 T1390 arg1Of transferred,to
R1136 T1389 T1396 arg1Of transferred,(
R1137 T1389 T1401 arg1Of transferred,for
R1138 T1393 T1394 arg1Of flask,or
R1139 T1394 T1390 arg2Of or,to
R1140 T1394 T1391 arg1Of or,a
R1141 T1394 T1392 arg1Of or,new
R1142 T1395 T1394 arg2Of dish,or
R1143 T1397 T1396 arg2Of Costar,(
R1144 T1397 T1398 arg1Of Costar,","
R1145 T1397 T1399 arg1Of Costar,USA
R1146 T1400 T1396 arg3Of ),(
R1147 T1402 T1401 arg2Of depleting,for
R1148 T1406 T1402 arg2Of cells,depleting
R1149 T1406 T1403 arg1Of cells,the
R1150 T1406 T1404 arg1Of cells,residual
R1151 T1406 T1405 arg1Of cells,epithelial
R1152 T1409 T1408 arg1Of cells,the
R1153 T1409 T1410 arg1Of cells,were
R1154 T1409 T1411 arg2Of cells,cultivated
R1155 T1411 T1407 arg1Of cultivated,Then
R1156 T1411 T1410 arg2Of cultivated,were
R1157 T1411 T1412 arg1Of cultivated,at
R1158 T1413 T1412 arg2Of 37°C,at
R1159 T1413 T1414 arg1Of 37°C,with
R1160 T1415 T1416 arg1Of 5,%
R1161 T1417 T1414 arg2Of CO2,with
R1162 T1417 T1415 arg1Of CO2,5
R1163 T1422 T1419 arg1Of medium,the
R1164 T1422 T1420 arg1Of medium,complete
R1165 T1422 T1421 arg1Of medium,culture
R1166 T1422 T1423 arg1Of medium,was
R1167 T1422 T1424 arg1Of medium,half-changed
R1168 T1423 T1418 arg1Of was,And
R1169 T1423 T1425 arg1Of was,twice
R1170 T1423 T1427 arg1Of was,week
R1171 T1424 T1423 arg2Of half-changed,was
R1172 T1427 T1426 arg1Of week,a
R1173 T1428 T1429 arg2Of Astrocytes,expanded
R1174 T1428 T1435 arg1Of Astrocytes,reach
R1175 T1429 T1430 arg1Of expanded,for
R1176 T1429 T1434 modOf expanded,to
R1177 T1432 T1431 arg1Of 7,about
R1178 T1433 T1430 arg2Of days,for
R1179 T1433 T1432 arg1Of days,7
R1180 T1435 T1434 arg1Of reach,to
R1181 T1436 T1435 arg2Of confluence,reach
R1182 T1439 T1440 arg1Of flasks,or
R1183 T1440 T1438 arg1Of or,the
R1184 T1440 T1442 arg1Of or,were
R1185 T1440 T1443 arg2Of or,shaken
R1186 T1441 T1440 arg2Of dishes,or
R1187 T1443 T1437 arg1Of shaken,Then
R1188 T1443 T1442 arg2Of shaken,were
R1189 T1443 T1444 arg1Of shaken,at
R1190 T1446 T1444 arg2Of rpm,at
R1191 T1446 T1445 arg1Of rpm,150
R1192 T1446 T1447 arg1Of rpm,for
R1193 T1449 T1447 arg2Of h,for
R1194 T1449 T1448 arg1Of h,4
R1195 T1449 T1451 arg1Of h,deplete
R1196 T1451 T1447 arg3Of deplete,for
R1197 T1451 T1450 arg1Of deplete,to
R1198 T1451 T1458 arg1Of deplete,from
R1199 T1453 T1454 arg1Of microglia,and
R1200 T1454 T1451 arg2Of and,deplete
R1201 T1454 T1452 arg1Of and,the
R1202 T1456 T1455 arg1Of adherent,less
R1203 T1457 T1454 arg2Of cells,and
R1204 T1457 T1456 arg1Of cells,adherent
R1205 T1460 T1458 arg2Of cultures,from
R1206 T1460 T1459 arg1Of cultures,the
R1207 T1462 T1461 arg2Of shaking,After
R1208 T1466 T1462 arg1Of cultures,shaking
R1209 T1466 T1464 arg1Of cultures,the
R1210 T1466 T1465 arg1Of cultures,resulting
R1211 T1466 T1467 arg1Of cultures,were
R1212 T1467 T1461 arg1Of were,After
R1213 T1467 T1463 arg1Of were,","
R1214 T1469 T1467 arg2Of astrocytes,were
R1215 T1469 T1468 arg1Of astrocytes,mainly
R1216 T1469 T1470 arg1Of astrocytes,","
R1217 T1469 T1471 arg1Of astrocytes,which
R1218 T1469 T1472 arg1Of astrocytes,were
R1219 T1469 T1473 arg2Of astrocytes,determined
R1220 T1473 T1472 arg2Of determined,were
R1221 T1473 T1474 arg1Of determined,by
R1222 T1475 T1474 arg2Of immunoreactivity,by
R1223 T1475 T1476 arg1Of immunoreactivity,for
R1224 T1477 T1476 arg2Of GFAP,for
R1225 T1477 T1478 arg1Of GFAP,(
R1226 T1479 T1480 arg1Of sc-166481,","
R1227 T1479 T1484 arg1Of sc-166481,","
R1228 T1483 T1480 arg2Of Biotechnology,","
R1229 T1483 T1481 arg1Of Biotechnology,Santa
R1230 T1483 T1482 arg1Of Biotechnology,Cruz
R1231 T1484 T1478 arg2Of ",",(
R1232 T1485 T1484 arg2Of CA,","
R1233 T1485 T1486 arg1Of CA,","
R1234 T1487 T1486 arg2Of USA,","
R1235 T1488 T1478 arg3Of ),(
R1236 T1489 T1490 arg2Of Cells,passaged
R1237 T1489 T1494 arg1Of Cells,were
R1238 T1489 T1495 arg2Of Cells,used
R1239 T1490 T1491 arg1Of passaged,for
R1240 T1493 T1491 arg2Of generations,for
R1241 T1493 T1492 arg1Of generations,2-3
R1242 T1495 T1494 arg2Of used,were
R1243 T1495 T1496 arg1Of used,in
R1244 T1499 T1496 arg2Of studies,in
R1245 T1499 T1497 arg1Of studies,the
R1246 T1499 T1498 arg1Of studies,following
R1526 T1824 T1822 arg2Of Model,In
R1527 T1824 T1823 arg1Of Model,Vitro
R1528 T1824 T1825 arg1Of Model,of
R1529 T1824 T1827 arg2Of Model,Established
R1530 T1826 T1825 arg2Of TBI,of
R1531 T1830 T1827 arg1Of Injury,Established
R1532 T1830 T1828 arg2Of Injury,by
R1533 T1830 T1829 arg1Of Injury,Scratch
R1534 T1833 T1831 arg1Of injury,Astrocyte
R1535 T1833 T1832 arg1Of injury,scratch
R1536 T1833 T1834 arg1Of injury,was
R1537 T1833 T1835 arg2Of injury,performed
R1538 T1835 T1834 arg2Of performed,was
R1539 T1835 T1837 arg1Of performed,in
R1540 T1835 T1841 arg1Of performed,[
R1541 T1837 T1836 arg1Of in,as
R1542 T1840 T1837 arg2Of report,in
R1543 T1840 T1838 arg1Of report,a
R1544 T1840 T1839 arg1Of report,previous
R1545 T1842 T1841 arg2Of 7,[
R1546 T1843 T1841 arg3Of ],[
R1547 T1844 T1845 arg1Of Astrocytes,were
R1548 T1844 T1846 arg2Of Astrocytes,planted
R1549 T1844 T1851 arg2Of Astrocytes,grown
R1550 T1846 T1847 arg1Of planted,in
R1551 T1846 T1850 arg1Of planted,and
R1552 T1849 T1847 arg2Of plates,in
R1553 T1849 T1848 arg1Of plates,6-well
R1554 T1850 T1845 arg2Of and,were
R1555 T1850 T1852 modOf and,to
R1556 T1851 T1850 arg2Of grown,and
R1557 T1853 T1852 arg1Of confluence,to
R1558 T1856 T1854 arg1Of monolayer,The
R1559 T1856 T1855 arg1Of monolayer,cell
R1560 T1856 T1857 arg1Of monolayer,was
R1561 T1856 T1858 arg2Of monolayer,scratched
R1562 T1858 T1857 arg2Of scratched,was
R1563 T1858 T1859 arg1Of scratched,with
R1564 T1858 T1865 arg1Of scratched,","
R1565 T1858 T1866 modOf scratched,resulting
R1566 T1864 T1859 arg2Of needle,with
R1567 T1864 T1860 arg1Of needle,a
R1568 T1864 T1861 arg1Of needle,sterile
R1569 T1864 T1862 arg1Of needle,26G
R1570 T1864 T1863 arg1Of needle,syringe
R1571 T1866 T1867 arg1Of resulting,in
R1572 T1869 T1867 arg2Of formation,in
R1573 T1869 T1868 arg1Of formation,the
R1574 T1869 T1870 arg1Of formation,of
R1575 T1875 T1870 arg2Of gap,of
R1576 T1875 T1871 arg1Of gap,a
R1577 T1875 T1872 arg1Of gap,0.5
R1578 T1875 T1873 arg1Of gap,mm
R1579 T1875 T1874 arg1Of gap,wide
R1580 T1877 T1876 arg1Of after,Immediately
R1581 T1878 T1877 arg2Of scratch,after
R1582 T1880 T1881 arg1Of cells,were
R1583 T1880 T1882 arg2Of cells,washed
R1584 T1880 T1895 arg2Of cells,named
R1585 T1882 T1883 arg1Of washed,twice
R1586 T1882 T1884 arg1Of washed,with
R1587 T1882 T1894 arg1Of washed,and
R1588 T1886 T1884 arg2Of PBS,with
R1589 T1886 T1885 arg1Of PBS,sterile
R1590 T1886 T1887 arg1Of PBS,","
R1591 T1886 T1888 arg2Of PBS,cultured
R1592 T1888 T1889 arg1Of cultured,with
R1593 T1892 T1889 arg2Of medium,with
R1594 T1892 T1890 arg1Of medium,complete
R1595 T1892 T1891 arg1Of medium,culture
R1596 T1894 T1877 arg1Of and,after
R1597 T1894 T1879 arg1Of and,","
R1598 T1894 T1881 arg2Of and,were
R1599 T1894 T1893 arg1Of and,","
R1600 T1895 T1894 arg2Of named,and
R1601 T1895 T1896 arg1Of named,as
R1602 T1898 T1896 arg2Of group,as
R1603 T1898 T1897 arg1Of group,TBI
R1604 T1899 T1900 arg1Of Cells,","
R1605 T1899 T1901 arg1Of Cells,which
R1606 T1899 T1902 arg1Of Cells,did
R1607 T1899 T1907 arg1Of Cells,were
R1608 T1899 T1908 arg2Of Cells,used
R1609 T1899 T1913 arg2Of Cells,named
R1610 T1902 T1903 arg1Of did,not
R1611 T1905 T1902 arg2Of scratch,did
R1612 T1905 T1904 arg2Of scratch,received
R1613 T1908 T1909 arg1Of used,as
R1614 T1908 T1912 arg1Of used,and
R1615 T1911 T1909 arg2Of control,as
R1616 T1911 T1910 arg1Of control,blank
R1617 T1912 T1906 arg1Of and,","
R1618 T1912 T1907 arg2Of and,were
R1619 T1913 T1912 arg2Of named,and
R1620 T1913 T1914 arg1Of named,as
R1621 T1916 T1914 arg2Of group,as
R1622 T1916 T1915 arg1Of group,sham
R1726 T2050 T2048 arg1Of Measurement,Cell
R1727 T2050 T2049 arg1Of Measurement,Death
R1728 T2050 T2051 arg1Of Measurement,by
R1729 T2053 T2051 arg2Of Assay,by
R1730 T2053 T2052 arg1Of Assay,CCK-8
R1731 T2057 T2054 arg1Of cells,1
R1732 T2057 T2055 arg1Of cells,×
R1733 T2057 T2056 arg1Of cells,103/well
R1734 T2057 T2058 arg1Of cells,were
R1735 T2057 T2059 arg2Of cells,seeded
R1736 T2057 T2065 arg2Of cells,cultivated
R1737 T2059 T2060 arg1Of seeded,to
R1738 T2059 T2064 arg1Of seeded,and
R1739 T2063 T2060 arg2Of plates,to
R1740 T2063 T2061 arg1Of plates,96-well
R1741 T2063 T2062 arg1Of plates,culture
R1742 T2064 T2058 arg2Of and,were
R1743 T2065 T2064 arg2Of cultivated,and
R1744 T2065 T2066 arg1Of cultivated,for
R1745 T2068 T2066 arg2Of h,for
R1746 T2068 T2067 arg1Of h,24
R1747 T2068 T2070 arg1Of h,adhere
R1748 T2070 T2066 arg3Of adhere,for
R1749 T2070 T2069 arg1Of adhere,to
R1750 T2073 T2072 arg1Of cell,the
R1751 T2073 T2074 arg1Of cell,was
R1752 T2073 T2075 arg2Of cell,scrated
R1753 T2075 T2071 arg1Of scrated,Then
R1754 T2075 T2074 arg2Of scrated,was
R1755 T2075 T2076 arg1Of scrated,as
R1756 T2077 T2076 arg2Of mentioned,as
R1757 T2077 T2078 arg1Of mentioned,above
R1758 T2081 T2079 arg1Of death,Astrocyte
R1759 T2081 T2080 arg1Of death,cell
R1760 T2081 T2082 arg1Of death,was
R1761 T2081 T2083 arg2Of death,assessed
R1762 T2083 T2082 arg2Of assessed,was
R1763 T2083 T2091 arg1Of assessed,(
R1764 T2083 T2101 arg1Of assessed,according
R1765 T2087 T2083 arg1Of kit-8,assessed
R1766 T2087 T2084 arg2Of kit-8,by
R1767 T2087 T2085 arg1Of kit-8,cell
R1768 T2087 T2086 arg1Of kit-8,counting
R1769 T2087 T2088 arg1Of kit-8,(
R1770 T2089 T2088 arg2Of CCK-8,(
R1771 T2090 T2088 arg3Of ),(
R1772 T2092 T2091 arg2Of Dojindo,(
R1773 T2092 T2093 arg1Of Dojindo,","
R1774 T2094 T2093 arg2Of Japan,","
R1775 T2095 T2091 arg3Of ),(
R1776 T2098 T2096 arg1Of h,assay
R1777 T2098 T2097 arg1Of h,24
R1778 T2098 T2099 arg1Of h,after
R1779 T2100 T2099 arg2Of scratch,after
R1780 T2101 T2098 arg1Of according,h
R1781 T2102 T2101 arg2Of to,according
R1782 T2104 T2103 arg1Of manufacturer,the
R1783 T2104 T2105 arg2Of manufacturer,'s
R1784 T2106 T2102 arg2Of protocol,to
R1785 T2106 T2105 arg1Of protocol,'s
R1786 T2107 T2108 arg1Of Briefly,","
R1787 T2107 T2112 arg1Of Briefly,was
R1788 T2107 T2113 arg2Of Briefly,added
R1789 T2107 T2118 arg2Of Briefly,incubated
R1790 T2111 T2108 arg2Of CCK-8,","
R1791 T2111 T2109 arg1Of CCK-8,10
R1792 T2111 T2110 arg1Of CCK-8,μL
R1793 T2113 T2114 arg1Of added,into
R1794 T2113 T2117 arg1Of added,and
R1795 T2116 T2114 arg2Of well,into
R1796 T2116 T2115 arg1Of well,every
R1797 T2117 T2112 arg2Of and,was
R1798 T2118 T2117 arg2Of incubated,and
R1799 T2118 T2119 arg1Of incubated,for
R1800 T2121 T2119 arg2Of h,for
R1801 T2121 T2120 arg1Of h,1
R1802 T2124 T2123 arg1Of value,OD
R1803 T2124 T2125 arg1Of value,was
R1804 T2124 T2126 arg2Of value,read
R1805 T2124 T2130 arg1Of value,using
R1806 T2126 T2122 arg1Of read,Then
R1807 T2126 T2125 arg2Of read,was
R1808 T2126 T2127 arg1Of read,at
R1809 T2126 T2130 modOf read,using
R1810 T2129 T2127 arg2Of nm,at
R1811 T2129 T2128 arg1Of nm,450
R1812 T2130 T2136 arg1Of using,(
R1813 T2135 T2130 arg2Of reader,using
R1814 T2135 T2131 arg1Of reader,a
R1815 T2135 T2132 arg1Of reader,Bio-Rad
R1816 T2135 T2133 arg1Of reader,ELISA
R1817 T2135 T2134 arg1Of reader,microplate
R1818 T2138 T2136 arg2Of Laboratories,(
R1819 T2138 T2137 arg1Of Laboratories,Bio-Rad
R1820 T2138 T2139 arg1Of Laboratories,","
R1821 T2140 T2139 arg2Of CA,","
R1822 T2140 T2141 arg1Of CA,","
R1823 T2142 T2141 arg2Of USA,","
R1824 T2143 T2136 arg3Of ),(
R1825 T2145 T2144 arg1Of measurements,All
R1826 T2145 T2146 arg1Of measurements,were
R1827 T2145 T2147 arg2Of measurements,performed
R1828 T2147 T2146 arg2Of performed,were
R1829 T2147 T2148 arg1Of performed,in
R1830 T2149 T2148 arg2Of sextuplicate,in
R1831 T2150 T2151 arg1Of Results,were
R1832 T2150 T2152 arg2Of Results,expressed
R1833 T2152 T2151 arg2Of expressed,were
R1834 T2152 T2153 arg1Of expressed,as
R1835 T2152 T2158 arg1Of expressed,at
R1836 T2154 T2153 arg2Of mean,as
R1837 T2154 T2155 arg1Of mean,of
R1838 T2157 T2155 arg2Of value,of
R1839 T2157 T2156 arg1Of value,OD
R1840 T2159 T2160 arg1Of 450,nm
R1841 T2162 T2158 arg2Of SD,at
R1842 T2162 T2159 arg1Of SD,450
R1843 T2162 T2161 arg1Of SD,±
R1968 T2327 T2324 arg1Of Assay,Electrophoretic
R1969 T2327 T2325 arg1Of Assay,Mobility
R1970 T2327 T2326 arg1Of Assay,Shift
R1971 T2327 T2328 arg1Of Assay,(
R1972 T2329 T2328 arg2Of EMSA,(
R1973 T2330 T2328 arg3Of ),(
R1974 T2331 T2332 arg1Of Monolayers,of
R1975 T2331 T2334 arg1Of Monolayers,were
R1976 T2331 T2335 arg2Of Monolayers,washed
R1977 T2331 T2339 arg2Of Monolayers,harvested
R1978 T2333 T2332 arg2Of astrocytes,of
R1979 T2335 T2336 arg1Of washed,with
R1980 T2335 T2338 arg1Of washed,and
R1981 T2337 T2336 arg2Of PBS,with
R1982 T2338 T2334 arg2Of and,were
R1983 T2339 T2338 arg2Of harvested,and
R1984 T2339 T2340 arg1Of harvested,by
R1985 T2341 T2340 arg2Of scraping,by
R1986 T2341 T2342 arg1Of scraping,into
R1987 T2344 T2342 arg2Of PBS,into
R1988 T2344 T2343 arg1Of PBS,cold
R1989 T2347 T2345 arg1Of pellet,The
R1990 T2347 T2346 arg1Of pellet,cell
R1991 T2347 T2348 arg2Of pellet,obtained
R1992 T2347 T2351 arg1Of pellet,was
R1993 T2347 T2352 arg2Of pellet,resuspended
R1994 T2350 T2348 arg1Of centrifugation,obtained
R1995 T2350 T2349 arg2Of centrifugation,by
R1996 T2352 T2351 arg2Of resuspended,was
R1997 T2352 T2353 arg1Of resuspended,in
R1998 T2354 T2355 arg1Of buffer,containing
R1999 T2354 T2367 arg1Of buffer,","
R2000 T2356 T2357 arg1Of 10,mM
R2001 T2358 T2356 arg1Of HEPES,10
R2002 T2358 T2359 arg1Of HEPES,(
R2003 T2358 T2363 arg1Of HEPES,","
R2004 T2360 T2359 arg2Of pH,(
R2005 T2360 T2361 arg1Of pH,7.9
R2006 T2362 T2359 arg3Of ),(
R2007 T2363 T2355 arg2Of ",",containing
R2008 T2364 T2365 arg1Of 10,mM
R2009 T2366 T2363 arg2Of KCl,","
R2010 T2366 T2364 arg1Of KCl,10
R2011 T2367 T2353 arg2Of ",",in
R2012 T2368 T2369 arg1Of 0.1,mM
R2013 T2370 T2368 arg1Of EDTA,0.1
R2014 T2370 T2371 arg1Of EDTA,","
R2015 T2371 T2375 arg1Of ",",","
R2016 T2372 T2373 arg1Of 0.1,mM
R2017 T2374 T2371 arg2Of EGTA,","
R2018 T2374 T2372 arg1Of EGTA,0.1
R2019 T2375 T2380 arg1Of ",",and
R2020 T2376 T2377 arg1Of 1.0,mM
R2021 T2378 T2375 arg2Of DTT,","
R2022 T2378 T2376 arg1Of DTT,1.0
R2023 T2380 T2367 arg2Of and,","
R2024 T2380 T2379 arg1Of and,","
R2025 T2381 T2382 arg1Of 0.5,mM
R2026 T2384 T2380 arg2Of fluoride,and
R2027 T2384 T2381 arg1Of fluoride,0.5
R2028 T2384 T2383 arg1Of fluoride,phenylmethylsulfonyl
R2029 T2386 T2385 arg1Of 10,Then
R2030 T2386 T2387 arg1Of 10,%
R2031 T2389 T2386 arg1Of P-40,10
R2032 T2389 T2388 arg1Of P-40,Nonidet
R2033 T2389 T2390 arg1Of P-40,was
R2034 T2389 T2391 arg2Of P-40,added
R2035 T2389 T2393 arg2Of P-40,vortexed
R2036 T2391 T2392 arg1Of added,and
R2037 T2392 T2390 arg2Of and,was
R2038 T2392 T2396 arg1Of and,and
R2039 T2393 T2392 arg2Of vortexed,and
R2040 T2394 T2393 arg3Of briefly,vortexed
R2041 T2396 T2395 arg1Of and,","
R2042 T2398 T2397 arg1Of nuclei,the
R2043 T2398 T2399 arg1Of nuclei,were
R2044 T2398 T2400 arg2Of nuclei,pelleted
R2045 T2400 T2396 arg2Of pelleted,and
R2046 T2400 T2399 arg2Of pelleted,were
R2047 T2402 T2400 arg1Of centrifugation,pelleted
R2048 T2402 T2401 arg2Of centrifugation,by
R2049 T2405 T2403 arg1Of proteins,The
R2050 T2405 T2404 arg1Of proteins,nuclear
R2051 T2405 T2406 arg1Of proteins,were
R2052 T2405 T2407 arg2Of proteins,extracted
R2053 T2407 T2406 arg2Of extracted,were
R2054 T2407 T2408 arg1Of extracted,with
R2055 T2409 T2410 arg1Of buffer,containing
R2056 T2409 T2422 arg1Of buffer,","
R2057 T2411 T2412 arg1Of 20,mM
R2058 T2413 T2411 arg1Of HEPES,20
R2059 T2413 T2414 arg1Of HEPES,(
R2060 T2413 T2418 arg1Of HEPES,","
R2061 T2415 T2414 arg2Of pH,(
R2062 T2415 T2416 arg1Of pH,7.9
R2063 T2417 T2414 arg3Of ),(
R2064 T2418 T2410 arg2Of ",",containing
R2065 T2419 T2420 arg1Of 0.4,mM
R2066 T2421 T2418 arg2Of NaCl,","
R2067 T2421 T2419 arg1Of NaCl,0.4
R2068 T2422 T2408 arg2Of ",",with
R2069 T2423 T2424 arg1Of 1.0,mM
R2070 T2425 T2423 arg1Of EDTA,1.0
R2071 T2425 T2426 arg1Of EDTA,","
R2072 T2426 T2430 arg1Of ",",","
R2073 T2427 T2428 arg1Of 1.0,mM
R2074 T2429 T2426 arg2Of EGTA,","
R2075 T2429 T2427 arg1Of EGTA,1.0
R2076 T2430 T2435 arg1Of ",",and
R2077 T2431 T2432 arg1Of 1.0,mM
R2078 T2433 T2430 arg2Of DTT,","
R2079 T2433 T2431 arg1Of DTT,1.0
R2080 T2435 T2422 arg2Of and,","
R2081 T2435 T2434 arg1Of and,","
R2082 T2436 T2437 arg1Of 1.0,mM
R2083 T2439 T2435 arg2Of fluoride,and
R2084 T2439 T2436 arg1Of fluoride,1.0
R2085 T2439 T2438 arg1Of fluoride,phenylmethylsulfonyl
R2086 T2441 T2440 arg1Of material,Insoluble
R2087 T2441 T2442 arg1Of material,was
R2088 T2441 T2443 arg2Of material,removed
R2089 T2443 T2442 arg2Of removed,was
R2090 T2443 T2446 arg1Of removed,at
R2091 T2443 T2450 arg1Of removed,and
R2092 T2445 T2443 arg1Of centrifugation,removed
R2093 T2445 T2444 arg2Of centrifugation,by
R2094 T2448 T2446 arg2Of rpm,at
R2095 T2448 T2447 arg1Of rpm,14000
R2096 T2450 T2449 arg1Of and,","
R2097 T2452 T2451 arg1Of supernatant,the
R2098 T2452 T2453 arg1Of supernatant,containing
R2099 T2452 T2457 arg1Of supernatant,was
R2100 T2452 T2458 arg2Of supernatant,stored
R2101 T2456 T2453 arg2Of proteins,containing
R2102 T2456 T2454 arg1Of proteins,the
R2103 T2456 T2455 arg1Of proteins,nuclear
R2104 T2458 T2450 arg2Of stored,and
R2105 T2458 T2457 arg2Of stored,was
R2106 T2458 T2459 arg1Of stored,at
R2107 T2458 T2461 arg1Of stored,until
R2108 T2460 T2459 arg2Of –80°C,at
R2109 T2462 T2461 arg2Of use,until
R2110 T2464 T2463 arg1Of concentration,Protein
R2111 T2464 T2465 arg1Of concentration,was
R2112 T2464 T2466 arg2Of concentration,determined
R2113 T2466 T2465 arg2Of determined,was
R2114 T2467 T2466 arg3Of using,determined
R2115 T2467 T2473 arg1Of using,with
R2116 T2472 T2467 arg2Of kit,using
R2117 T2472 T2468 arg1Of kit,a
R2118 T2472 T2469 arg1Of kit,bicinchoninic
R2119 T2472 T2470 arg1Of kit,acid
R2120 T2472 T2471 arg1Of kit,assay
R2121 T2476 T2473 arg2Of albumin,with
R2122 T2476 T2474 arg1Of albumin,bovine
R2123 T2476 T2475 arg1Of albumin,serum
R2124 T2476 T2477 arg1Of albumin,as
R2125 T2482 T2481 arg1Of Biochemicals,Pierce
R2126 T2482 T2483 arg1Of Biochemicals,","
R2127 T2483 T2485 arg1Of ",",","
R2128 T2484 T2483 arg2Of Rockford,","
R2129 T2485 T2477 arg2Of ",",as
R2130 T2485 T2478 arg1Of ",",the
R2131 T2485 T2479 arg1Of ",",standard
R2132 T2485 T2480 arg2Of ",",(
R2133 T2485 T2487 arg1Of ",",","
R2134 T2485 T2488 arg1Of ",",USA
R2135 T2486 T2485 arg2Of IL,","
R2136 T2489 T2480 arg3Of ),(
R2137 T2490 T2491 arg1Of EMSA,was
R2138 T2490 T2492 arg2Of EMSA,performed
R2139 T2492 T2491 arg2Of performed,was
R2140 T2493 T2492 arg3Of using,performed
R2141 T2497 T2493 arg2Of system,using
R2142 T2497 T2494 arg1Of system,gel
R2143 T2497 T2495 arg1Of system,shift
R2144 T2497 T2496 arg1Of system,assay
R2145 T2497 T2498 arg1Of system,(
R2146 T2499 T2500 arg1Of Promega,","
R2147 T2500 T2502 arg1Of ",",","
R2148 T2501 T2500 arg2Of Madison,","
R2149 T2502 T2498 arg2Of ",",(
R2150 T2502 T2504 arg1Of ",",","
R2151 T2502 T2505 arg1Of ",",USA
R2152 T2503 T2502 arg2Of WI,","
R2153 T2506 T2498 arg3Of ),(
R2154 T2509 T2507 arg1Of probe,Consensus
R2155 T2509 T2508 arg1Of probe,oligonucleotide
R2156 T2509 T2510 arg1Of probe,(
R2157 T2509 T2514 arg1Of probe,was
R2158 T2509 T2515 arg2Of probe,end-labeled
R2159 T2509 T2519 arg1Of probe,using
R2160 T2511 T2510 arg2Of 5′-AGTTGAGGGGACTTTCCCAGGG-3,(
R2161 T2511 T2512 arg1Of 5′-AGTTGAGGGGACTTTCCCAGGG-3,′
R2162 T2513 T2510 arg3Of ),(
R2163 T2515 T2514 arg2Of end-labeled,was
R2164 T2515 T2519 modOf end-labeled,using
R2165 T2518 T2515 arg1Of kinase,end-labeled
R2166 T2518 T2516 arg2Of kinase,by
R2167 T2518 T2517 arg1Of kinase,T4-polynucleotide
R2168 T2521 T2520 arg2Of γ-32P,[
R2169 T2522 T2520 arg3Of ],[
R2170 T2523 T2519 arg2Of -ATP,using
R2171 T2523 T2520 arg1Of -ATP,[
R2172 T2523 T2524 arg1Of -ATP,(
R2173 T2526 T2524 arg2Of Biotech.,(
R2174 T2526 T2525 arg1Of Biotech.,Free
R2175 T2526 T2527 arg1Of Biotech.,","
R2176 T2528 T2527 arg2Of Beijing,","
R2177 T2528 T2529 arg1Of Beijing,","
R2178 T2530 T2529 arg2Of China,","
R2179 T2531 T2524 arg3Of ),(
R2180 T2533 T2532 arg1Of protein,Nuclear
R2181 T2533 T2534 arg1Of protein,(
R2182 T2533 T2538 arg1Of protein,was
R2183 T2533 T2539 arg2Of protein,preincubated
R2184 T2536 T2534 arg2Of μg,(
R2185 T2536 T2535 arg1Of μg,20
R2186 T2537 T2534 arg3Of ),(
R2187 T2539 T2538 arg2Of preincubated,was
R2188 T2539 T2540 arg1Of preincubated,in
R2189 T2544 T2540 arg2Of buffer,in
R2190 T2544 T2541 arg1Of buffer,20
R2191 T2544 T2542 arg1Of buffer,μL
R2192 T2544 T2543 arg1Of buffer,binding
R2193 T2544 T2545 arg1Of buffer,containing
R2194 T2546 T2547 arg1Of 10,mM
R2195 T2548 T2546 arg1Of Tris-HCl,10
R2196 T2548 T2549 arg1Of Tris-HCl,(
R2197 T2548 T2553 arg1Of Tris-HCl,","
R2198 T2550 T2549 arg2Of PH,(
R2199 T2550 T2551 arg1Of PH,7.5
R2200 T2552 T2549 arg3Of ),(
R2201 T2553 T2557 arg1Of ",",","
R2202 T2554 T2555 arg1Of 1,mM
R2203 T2556 T2553 arg2Of MgCl2,","
R2204 T2556 T2554 arg1Of MgCl2,1
R2205 T2557 T2561 arg1Of ",",","
R2206 T2558 T2559 arg1Of 0.5,mM
R2207 T2560 T2557 arg2Of NaCl,","
R2208 T2560 T2558 arg1Of NaCl,0.5
R2209 T2561 T2545 arg2Of ",",containing
R2210 T2562 T2563 arg1Of 4,%
R2211 T2564 T2562 arg1Of glycerol,4
R2212 T2564 T2565 arg1Of glycerol,","
R2213 T2565 T2569 arg1Of ",",","
R2214 T2566 T2567 arg1Of 0.5,mM
R2215 T2568 T2565 arg2Of EDTA,","
R2216 T2568 T2566 arg1Of EDTA,0.5
R2217 T2569 T2574 arg1Of ",",and
R2218 T2570 T2571 arg1Of 0.5,mM
R2219 T2572 T2569 arg2Of DTT,","
R2220 T2572 T2570 arg1Of DTT,0.5
R2221 T2574 T2561 arg2Of and,","
R2222 T2574 T2573 arg1Of and,","
R2223 T2578 T2574 arg2Of dI-dC,and
R2224 T2578 T2575 arg1Of dI-dC,2
R2225 T2578 T2576 arg1Of dI-dC,μg
R2226 T2578 T2577 arg1Of dI-dC,poly
R2227 T2578 T2579 arg1Of dI-dC,for
R2228 T2581 T2579 arg2Of minutes,for
R2229 T2581 T2580 arg1Of minutes,20
R2230 T2581 T2582 arg1Of minutes,on
R2231 T2583 T2582 arg2Of ice,on
R2232 T2585 T2584 arg2Of addition,After
R2233 T2585 T2586 arg1Of addition,of
R2234 T2592 T2586 arg2Of probe,of
R2235 T2592 T2587 arg1Of probe,the
R2236 T2592 T2588 arg1Of probe,1
R2237 T2592 T2589 arg1Of probe,μL
R2238 T2592 T2590 arg1Of probe,32P-labled
R2239 T2592 T2591 arg1Of probe,oligonucleotide
R2240 T2595 T2594 arg1Of incubation,the
R2241 T2595 T2596 arg1Of incubation,was
R2242 T2595 T2597 arg2Of incubation,continued
R2243 T2597 T2584 arg1Of continued,After
R2244 T2597 T2593 arg1Of continued,","
R2245 T2597 T2596 arg2Of continued,was
R2246 T2597 T2598 arg1Of continued,for
R2247 T2600 T2598 arg2Of minutes,for
R2248 T2600 T2599 arg1Of minutes,20
R2249 T2600 T2601 arg1Of minutes,on
R2250 T2602 T2601 arg2Of ice,on
R2251 T2605 T2603 arg1Of complexes,The
R2252 T2605 T2604 arg1Of complexes,DNA-protein
R2253 T2605 T2606 arg1Of complexes,were
R2254 T2605 T2607 arg2Of complexes,separated
R2255 T2607 T2606 arg2Of separated,were
R2256 T2607 T2610 arg1Of separated,on
R2257 T2607 T2616 arg1Of separated,in
R2258 T2609 T2607 arg1Of electrophoresis,separated
R2259 T2609 T2608 arg2Of electrophoresis,by
R2260 T2611 T2612 arg1Of 4,%
R2261 T2615 T2610 arg2Of gel,on
R2262 T2615 T2611 arg1Of gel,4
R2263 T2615 T2613 arg1Of gel,nondenaturing
R2264 T2615 T2614 arg1Of gel,polyacrylamide
R2265 T2620 T2616 arg2Of buffer,in
R2266 T2620 T2617 arg1Of buffer,0.5
R2267 T2620 T2618 arg1Of buffer,×
R2268 T2620 T2619 arg1Of buffer,TBE
R2269 T2620 T2621 arg1Of buffer,(
R2270 T2620 T2624 arg1Of buffer,at
R2271 T2622 T2621 arg2Of tris-borate-EDTA,(
R2272 T2623 T2621 arg3Of ),(
R2273 T2626 T2624 arg2Of V,at
R2274 T2626 T2625 arg1Of V,390
R2275 T2626 T2627 arg1Of V,for
R2276 T2629 T2627 arg2Of hour,for
R2277 T2629 T2628 arg1Of hour,1
R2278 T2629 T2630 arg1Of hour,at
R2279 T2631 T2630 arg2Of 4°C,at
R2280 T2633 T2632 arg2Of electrophoresis,After
R2281 T2636 T2635 arg1Of gel,the
R2282 T2636 T2637 arg1Of gel,was
R2283 T2636 T2638 arg2Of gel,dried
R2284 T2636 T2640 arg2Of gel,exposed
R2285 T2638 T2639 arg1Of dried,and
R2286 T2639 T2632 arg1Of and,After
R2287 T2639 T2634 arg1Of and,","
R2288 T2639 T2637 arg2Of and,was
R2289 T2640 T2639 arg2Of exposed,and
R2290 T2640 T2641 arg1Of exposed,to
R2291 T2643 T2641 arg2Of film,to
R2292 T2643 T2642 arg1Of film,X-ray
R2293 T2643 T2644 arg1Of film,(
R2294 T2646 T2644 arg2Of Hyperfilm,(
R2295 T2646 T2645 arg1Of Hyperfilm,Fuji
R2296 T2646 T2647 arg1Of Hyperfilm,","
R2297 T2648 T2647 arg2Of Tokyo,","
R2298 T2648 T2649 arg1Of Tokyo,","
R2299 T2650 T2649 arg2Of Japan,","
R2300 T2651 T2644 arg3Of ),(
R2301 T2652 T2653 arg1Of Levels,of
R2302 T2652 T2658 arg1Of Levels,were
R2303 T2652 T2659 arg2Of Levels,quantified
R2304 T2657 T2653 arg2Of activity,of
R2305 T2657 T2654 arg1Of activity,NF-κB
R2306 T2657 T2655 arg1Of activity,DNA
R2307 T2657 T2656 arg1Of activity,binding
R2308 T2659 T2658 arg2Of quantified,were
R2309 T2662 T2659 arg1Of ImageJ,quantified
R2310 T2662 T2660 arg2Of ImageJ,by
R2311 T2662 T2661 arg1Of ImageJ,software
R2677 T3118 T3117 arg1Of RNA,Total
R2678 T3118 T3119 arg1Of RNA,was
R2679 T3118 T3120 arg2Of RNA,isolated
R2680 T3120 T3119 arg2Of isolated,was
R2681 T3120 T3121 arg1Of isolated,with
R2682 T3120 T3131 arg1Of isolated,and
R2683 T3122 T3121 arg2Of Trizol,with
R2684 T3122 T3123 arg1Of Trizol,(
R2685 T3124 T3123 arg2Of Invitrogen,(
R2686 T3124 T3125 arg1Of Invitrogen,","
R2687 T3124 T3127 arg1Of Invitrogen,","
R2688 T3124 T3128 arg1Of Invitrogen,USA
R2689 T3126 T3125 arg2Of CA,","
R2690 T3129 T3123 arg3Of ),(
R2691 T3131 T3130 arg1Of and,","
R2692 T3131 T3145 arg1Of and,(
R2693 T3131 T3152 arg1Of and,according
R2694 T3133 T3132 arg1Of cDNA,single-stranded
R2695 T3133 T3134 arg1Of cDNA,was
R2696 T3133 T3135 arg2Of cDNA,synthesized
R2697 T3135 T3131 arg2Of synthesized,and
R2698 T3135 T3134 arg2Of synthesized,was
R2699 T3135 T3136 arg1Of synthesized,from
R2700 T3138 T3136 arg2Of μg,from
R2701 T3138 T3137 arg1Of μg,2
R2702 T3138 T3139 arg1Of μg,of
R2703 T3141 T3139 arg2Of RNA,of
R2704 T3141 T3140 arg1Of RNA,total
R2705 T3141 T3142 arg1Of RNA,with
R2706 T3144 T3142 arg2Of RT-Kit,with
R2707 T3144 T3143 arg1Of RT-Kit,BU-Script
R2708 T3146 T3147 arg1Of Biunique,","
R2709 T3147 T3145 arg2Of ",",(
R2710 T3147 T3149 arg1Of ",",","
R2711 T3148 T3147 arg2Of Jiangsu,","
R2712 T3150 T3149 arg2Of China,","
R2713 T3151 T3145 arg3Of ),(
R2714 T3153 T3152 arg2Of to,according
R2715 T3155 T3154 arg1Of manufacturer,the
R2716 T3155 T3156 arg2Of manufacturer,'s
R2717 T3157 T3153 arg2Of protocol,to
R2718 T3157 T3156 arg1Of protocol,'s
R2719 T3159 T3158 arg1Of cDNA,The
R2720 T3159 T3160 arg1Of cDNA,was
R2721 T3159 T3161 arg2Of cDNA,stored
R2722 T3161 T3160 arg2Of stored,was
R2723 T3161 T3162 arg1Of stored,in
R2724 T3163 T3162 arg2Of −20°C,in
R2725 T3165 T3164 arg1Of transcription,Reverse
R2726 T3165 T3166 arg1Of transcription,was
R2727 T3165 T3167 arg2Of transcription,conducted
R2728 T3167 T3166 arg2Of conducted,was
R2729 T3167 T3168 arg1Of conducted,with
R2730 T3167 T3180 arg1Of conducted,according
R2731 T3172 T3168 arg2Of Mix,with
R2732 T3172 T3169 arg1Of Mix,GoTaq
R2733 T3172 T3170 arg1Of Mix,Green
R2734 T3172 T3171 arg1Of Mix,Master
R2735 T3172 T3173 arg1Of Mix,(
R2736 T3174 T3175 arg1Of Promega,","
R2737 T3175 T3173 arg2Of ",",(
R2738 T3175 T3177 arg1Of ",",","
R2739 T3175 T3178 arg1Of ",",USA
R2740 T3176 T3175 arg2Of WI,","
R2741 T3179 T3173 arg3Of ),(
R2742 T3181 T3180 arg2Of to,according
R2743 T3183 T3182 arg1Of manufacturer,the
R2744 T3183 T3184 arg2Of manufacturer,'s
R2745 T3185 T3181 arg2Of protocol,to
R2746 T3185 T3184 arg1Of protocol,'s
R2747 T3187 T3186 arg1Of 1,Table
R2748 T3187 T3188 arg1Of 1,shows
R2749 T3190 T3191 arg1Of primers,and
R2750 T3191 T3188 arg2Of and,shows
R2751 T3191 T3189 arg1Of and,the
R2752 T3193 T3191 arg2Of parameters,and
R2753 T3193 T3192 arg1Of parameters,PCR
R2754 T3195 T3194 arg1Of products,PCR
R2755 T3195 T3196 arg1Of products,were
R2756 T3195 T3197 arg2Of products,detected
R2757 T3197 T3196 arg2Of detected,were
R2758 T3201 T3197 arg1Of electrophoresis,detected
R2759 T3201 T3198 arg2Of electrophoresis,by
R2760 T3201 T3199 arg1Of electrophoresis,agarose
R2761 T3201 T3200 arg1Of electrophoresis,gel
R2762 T3203 T3202 arg1Of intensity,The
R2763 T3203 T3204 arg1Of intensity,of
R2764 T3203 T3207 arg1Of intensity,was
R2765 T3203 T3208 arg2Of intensity,analyzed
R2766 T3206 T3204 arg2Of bands,of
R2767 T3206 T3205 arg1Of bands,the
R2768 T3208 T3207 arg2Of analyzed,was
R2769 T3211 T3208 arg1Of program,analyzed
R2770 T3211 T3209 arg2Of program,by
R2771 T3211 T3210 arg1Of program,ImageJ
R2772 T3213 T3212 arg1Of level,The
R2773 T3213 T3214 arg1Of level,of
R2774 T3213 T3216 arg1Of level,was
R2775 T3213 T3217 arg2Of level,used
R2776 T3215 T3214 arg2Of β-actin,of
R2777 T3217 T3216 arg2Of used,was
R2778 T3217 T3218 arg1Of used,as
R2779 T3221 T3218 arg2Of standard,as
R2780 T3221 T3219 arg1Of standard,an
R2781 T3221 T3220 arg1Of standard,internal
R2899 T3391 T3390 arg1Of Blot,Western
R2900 T3393 T3392 arg1Of obtain,To
R2901 T3396 T3393 arg2Of lysates,obtain
R2902 T3396 T3394 arg1Of lysates,total
R2903 T3396 T3395 arg1Of lysates,protein
R2904 T3398 T3393 arg1Of cells,obtain
R2905 T3398 T3399 arg1Of cells,were
R2906 T3398 T3400 arg2Of cells,homogenized
R2907 T3398 T3452 arg2Of cells,centrifuged
R2908 T3400 T3401 arg1Of homogenized,in
R2909 T3400 T3451 arg1Of homogenized,and
R2910 T3403 T3401 arg2Of buffer,in
R2911 T3403 T3402 arg1Of buffer,RIPA
R2912 T3403 T3404 arg1Of buffer,(
R2913 T3405 T3406 arg1Of 1,%
R2914 T3407 T3405 arg1Of NP40,1
R2915 T3407 T3408 arg1Of NP40,","
R2916 T3408 T3413 arg1Of ",",","
R2917 T3409 T3410 arg1Of 0.5,%
R2918 T3412 T3408 arg2Of deoxycholate,","
R2919 T3412 T3409 arg1Of deoxycholate,0.5
R2920 T3412 T3411 arg1Of deoxycholate,sodium
R2921 T3413 T3417 arg1Of ",",","
R2922 T3414 T3415 arg1Of 0.1,%
R2923 T3416 T3413 arg2Of SDS,","
R2924 T3416 T3414 arg1Of SDS,0.1
R2925 T3417 T3421 arg1Of ",",","
R2926 T3418 T3419 arg1Of 1,mM
R2927 T3420 T3417 arg2Of EDTA,","
R2928 T3420 T3418 arg1Of EDTA,1
R2929 T3421 T3404 arg2Of ",",(
R2930 T3421 T3425 arg1Of ",",","
R2931 T3422 T3423 arg1Of 1,mM
R2932 T3424 T3421 arg2Of EGTA,","
R2933 T3424 T3422 arg1Of EGTA,1
R2934 T3426 T3427 arg1Of 1,mM
R2935 T3428 T3426 arg1Of Na3VO4,1
R2936 T3428 T3429 arg1Of Na3VO4,","
R2937 T3429 T3433 arg1Of ",",","
R2938 T3430 T3431 arg1Of 20,mM
R2939 T3432 T3429 arg2Of NaF,","
R2940 T3432 T3430 arg1Of NaF,20
R2941 T3433 T3437 arg1Of ",",","
R2942 T3434 T3435 arg1Of 0.5,mM
R2943 T3436 T3433 arg2Of DTT,","
R2944 T3436 T3434 arg1Of DTT,0.5
R2945 T3437 T3442 arg1Of ",",and
R2946 T3438 T3439 arg1Of 1,mM
R2947 T3440 T3437 arg2Of PMSF,","
R2948 T3440 T3438 arg1Of PMSF,1
R2949 T3442 T3425 arg2Of and,","
R2950 T3442 T3441 arg1Of and,","
R2951 T3445 T3442 arg2Of cocktail,and
R2952 T3445 T3443 arg1Of cocktail,protease
R2953 T3445 T3444 arg1Of cocktail,inhibitor
R2954 T3445 T3446 arg1Of cocktail,in
R2955 T3448 T3446 arg2Of pH,in
R2956 T3448 T3447 arg1Of pH,PBS
R2957 T3448 T3449 arg1Of pH,7.4
R2958 T3450 T3404 arg3Of ),(
R2959 T3451 T3392 modOf and,To
R2960 T3451 T3397 arg1Of and,","
R2961 T3451 T3399 arg2Of and,were
R2962 T3452 T3451 arg2Of centrifuged,and
R2963 T3452 T3453 arg1Of centrifuged,at
R2964 T3452 T3456 arg1Of centrifuged,for
R2965 T3455 T3453 arg2Of g,at
R2966 T3455 T3454 arg1Of g,"12,000"
R2967 T3458 T3456 arg2Of min,for
R2968 T3458 T3457 arg1Of min,15
R2969 T3458 T3459 arg1Of min,at
R2970 T3460 T3459 arg2Of 4°C,at
R2971 T3462 T3461 arg1Of concentrations,Protein
R2972 T3462 T3463 arg1Of concentrations,were
R2973 T3462 T3464 arg2Of concentrations,estimated
R2974 T3464 T3463 arg2Of estimated,were
R2975 T3470 T3464 arg1Of Reagent,estimated
R2976 T3470 T3465 arg2Of Reagent,by
R2977 T3470 T3466 arg1Of Reagent,Coomassie
R2978 T3470 T3467 arg1Of Reagent,Plus
R2979 T3470 T3468 arg1Of Reagent,Protein
R2980 T3470 T3469 arg1Of Reagent,Assay
R2981 T3470 T3471 arg1Of Reagent,(
R2982 T3472 T3473 arg1Of Pierce,","
R2983 T3473 T3471 arg2Of ",",(
R2984 T3473 T3475 arg1Of ",",","
R2985 T3474 T3473 arg2Of IL,","
R2986 T3476 T3475 arg2Of USA,","
R2987 T3477 T3471 arg3Of ),(
R2988 T3479 T3478 arg1Of micrograms,Fifty
R2989 T3479 T3480 arg1Of micrograms,of
R2990 T3479 T3486 arg1Of micrograms,were
R2991 T3479 T3487 arg1Of micrograms,heat-denatured
R2992 T3479 T3506 arg1Of micrograms,electroblotted
R2993 T3485 T3480 arg2Of extracts,of
R2994 T3485 T3481 arg1Of extracts,the
R2995 T3485 T3482 arg1Of extracts,resulting
R2996 T3485 T3483 arg1Of extracts,cytosolic
R2997 T3485 T3484 arg1Of extracts,protein
R2998 T3486 T3488 arg1Of were,in
R2999 T3486 T3505 arg1Of were,and
R3000 T3487 T3486 arg2Of heat-denatured,were
R3001 T3492 T3488 arg2Of buffer,in
R3002 T3492 T3489 arg1Of buffer,Laemmli
R3003 T3492 T3490 arg1Of buffer,sample
R3004 T3492 T3491 arg1Of buffer,loading
R3005 T3492 T3493 arg1Of buffer,","
R3006 T3492 T3494 arg2Of buffer,separated
R3007 T3496 T3497 arg1Of 10,%
R3008 T3503 T3494 arg1Of electrophoresis,separated
R3009 T3503 T3495 arg2Of electrophoresis,by
R3010 T3503 T3496 arg1Of electrophoresis,10
R3011 T3503 T3498 arg1Of electrophoresis,sodium
R3012 T3503 T3499 arg1Of electrophoresis,dodecyl
R3013 T3503 T3500 arg1Of electrophoresis,sulfate
R3014 T3503 T3501 arg1Of electrophoresis,polyacrylamide
R3015 T3503 T3502 arg1Of electrophoresis,gel
R3016 T3505 T3504 arg1Of and,","
R3017 T3506 T3505 arg2Of electroblotted,and
R3018 T3506 T3507 arg1Of electroblotted,onto
R3019 T3510 T3507 arg2Of membrane,onto
R3020 T3510 T3508 arg1Of membrane,a
R3021 T3510 T3509 arg1Of membrane,nitrocellulose
R3022 T3512 T3511 arg2Of immunoblotting,For
R3023 T3514 T3515 arg1Of membranes,were
R3024 T3514 T3516 arg2Of membranes,blocked
R3025 T3516 T3511 arg1Of blocked,For
R3026 T3516 T3513 arg1Of blocked,","
R3027 T3516 T3515 arg2Of blocked,were
R3028 T3516 T3517 arg1Of blocked,with
R3029 T3516 T3526 arg1Of blocked,overnight
R3030 T3516 T3527 arg1Of blocked,at
R3031 T3516 T3530 arg1Of blocked,and
R3032 T3518 T3519 arg1Of 5,%
R3033 T3522 T3517 arg2Of milk,with
R3034 T3522 T3518 arg1Of milk,5
R3035 T3522 T3520 arg1Of milk,nonfat
R3036 T3522 T3521 arg1Of milk,dry
R3037 T3522 T3523 arg1Of milk,in
R3038 T3525 T3523 arg2Of buffer,in
R3039 T3525 T3524 arg1Of buffer,saline
R3040 T3528 T3527 arg2Of 4°C,at
R3041 T3530 T3529 arg1Of and,","
R3042 T3530 T3536 arg1Of and,:
R3043 T3530 T3537 arg1Of and,anti-β-actin
R3044 T3530 T3554 arg1Of and,metallopeptidase
R3045 T3533 T3531 arg1Of antibodies,the
R3046 T3533 T3532 arg1Of antibodies,following
R3047 T3533 T3534 arg1Of antibodies,were
R3048 T3533 T3535 arg2Of antibodies,used
R3049 T3535 T3530 arg2Of used,and
R3050 T3535 T3534 arg2Of used,were
R3051 T3537 T3538 arg1Of anti-β-actin,(
R3052 T3537 T3552 arg1Of anti-β-actin,and
R3053 T3539 T3540 arg1Of sc-130657,","
R3054 T3539 T3544 arg1Of sc-130657,","
R3055 T3543 T3540 arg2Of Biotechnology,","
R3056 T3543 T3541 arg1Of Biotechnology,Santa
R3057 T3543 T3542 arg1Of Biotechnology,Cruz
R3058 T3544 T3538 arg2Of ",",(
R3059 T3545 T3546 arg1Of CA,","
R3060 T3546 T3548 arg1Of ",",","
R3061 T3547 T3546 arg2Of USA,","
R3062 T3549 T3548 arg2Of 43,","
R3063 T3550 T3544 arg2Of kDa,","
R3064 T3550 T3545 arg1Of kDa,CA
R3065 T3550 T3547 arg1Of kDa,USA
R3066 T3550 T3549 arg1Of kDa,43
R3067 T3551 T3538 arg3Of ),(
R3068 T3552 T3559 arg1Of and,(
R3069 T3554 T3552 arg2Of metallopeptidase,and
R3070 T3554 T3553 arg1Of metallopeptidase,anti-matrix
R3071 T3554 T3555 arg1Of metallopeptidase,9
R3072 T3554 T3556 arg1Of metallopeptidase,(
R3073 T3557 T3556 arg2Of MMP9,(
R3074 T3558 T3556 arg3Of ),(
R3075 T3560 T3561 arg1Of sc-6841,","
R3076 T3560 T3565 arg1Of sc-6841,","
R3077 T3564 T3561 arg2Of Biotechnology,","
R3078 T3564 T3562 arg1Of Biotechnology,Santa
R3079 T3564 T3563 arg1Of Biotechnology,Cruz
R3080 T3565 T3559 arg2Of ",",(
R3081 T3566 T3567 arg1Of CA,","
R3082 T3567 T3569 arg1Of ",",","
R3083 T3568 T3567 arg2Of USA,","
R3084 T3570 T3569 arg2Of 92,","
R3085 T3571 T3565 arg2Of kDa,","
R3086 T3571 T3566 arg1Of kDa,CA
R3087 T3571 T3568 arg1Of kDa,USA
R3088 T3571 T3570 arg1Of kDa,92
R3089 T3572 T3559 arg3Of ),(
R3090 T3575 T3573 arg1Of antibody,Each
R3091 T3575 T3574 arg1Of antibody,primary
R3092 T3575 T3576 arg1Of antibody,was
R3093 T3575 T3577 arg2Of antibody,diluted
R3094 T3575 T3584 arg1Of antibody,added
R3095 T3577 T3576 arg2Of diluted,was
R3096 T3577 T3578 arg1Of diluted,appropriately
R3097 T3577 T3579 arg1Of diluted,in
R3098 T3577 T3582 arg1Of diluted,and
R3099 T3580 T3579 arg2Of blocking,in
R3100 T3581 T3580 arg2Of buffer,blocking
R3101 T3584 T3582 arg2Of added,and
R3102 T3584 T3583 arg1Of added,then
R3103 T3584 T3585 arg1Of added,to
R3104 T3584 T3588 arg1Of added,for
R3105 T3587 T3585 arg2Of blots,to
R3106 T3587 T3586 arg1Of blots,the
R3107 T3590 T3588 arg2Of h,for
R3108 T3590 T3589 arg1Of h,1
R3109 T3590 T3591 arg1Of h,at
R3110 T3593 T3591 arg2Of temperature,at
R3111 T3593 T3592 arg1Of temperature,room
R3112 T3595 T3594 arg1Of blots,The
R3113 T3595 T3596 arg1Of blots,were
R3114 T3595 T3597 arg2Of blots,washed
R3115 T3595 T3605 arg2Of blots,covered
R3116 T3597 T3599 arg1Of washed,times
R3117 T3597 T3600 arg1Of washed,in
R3118 T3597 T3604 arg1Of washed,and
R3119 T3599 T3598 arg1Of times,three
R3120 T3603 T3600 arg2Of buffer,in
R3121 T3603 T3601 arg1Of buffer,the
R3122 T3603 T3602 arg1Of buffer,washing
R3123 T3604 T3596 arg2Of and,were
R3124 T3605 T3604 arg2Of covered,and
R3125 T3605 T3606 arg1Of covered,with
R3126 T3605 T3612 arg1Of covered,at
R3127 T3611 T3606 arg2Of antibody,with
R3128 T3611 T3607 arg1Of antibody,the
R3129 T3611 T3608 arg1Of antibody,horseradish
R3130 T3611 T3609 arg1Of antibody,peroxidase-linked
R3131 T3611 T3610 arg1Of antibody,secondary
R3132 T3614 T3612 arg2Of 1,at
R3133 T3614 T3613 arg1Of 1,a
R3134 T3614 T3615 arg1Of 1,:
R3135 T3617 T3616 arg1Of dilution,2000
R3136 T3617 T3618 arg1Of dilution,for
R3137 T3620 T3618 arg2Of h,for
R3138 T3620 T3619 arg1Of h,1
R3139 T3621 T3622 arg1Of Blots,were
R3140 T3621 T3623 arg2Of Blots,incubated
R3141 T3621 T3641 arg2Of Blots,exposed
R3142 T3623 T3624 arg1Of incubated,with
R3143 T3623 T3640 arg1Of incubated,and
R3144 T3628 T3627 arg2Of ECL,(
R3145 T3629 T3627 arg3Of ),(
R3146 T3631 T3624 arg2Of system,with
R3147 T3631 T3625 arg2Of system,enhanced
R3148 T3631 T3626 arg1Of system,chemiluminescence
R3149 T3631 T3627 arg1Of system,(
R3150 T3631 T3630 arg1Of system,detection
R3151 T3631 T3632 arg1Of system,(
R3152 T3634 T3632 arg2Of Biosciences,(
R3153 T3634 T3633 arg1Of Biosciences,Amersham
R3154 T3634 T3635 arg1Of Biosciences,","
R3155 T3636 T3635 arg2Of Bucks,","
R3156 T3636 T3637 arg1Of Bucks,","
R3157 T3638 T3637 arg2Of UK,","
R3158 T3639 T3632 arg3Of ),(
R3159 T3640 T3622 arg2Of and,were
R3160 T3641 T3640 arg2Of exposed,and
R3161 T3641 T3642 arg1Of exposed,to
R3162 T3644 T3642 arg2Of film,to
R3163 T3644 T3643 arg1Of film,radiographic
R3164 T3644 T3645 arg1Of film,(
R3165 T3647 T3645 arg2Of Hyperfilm,(
R3166 T3647 T3646 arg1Of Hyperfilm,Fuji
R3167 T3647 T3648 arg1Of Hyperfilm,","
R3168 T3649 T3648 arg2Of Tokyo,","
R3169 T3649 T3650 arg1Of Tokyo,","
R3170 T3651 T3650 arg2Of Japan,","
R3171 T3652 T3645 arg3Of ),(
R3172 T3653 T3654 arg1Of ImageJ,was
R3173 T3653 T3655 arg2Of ImageJ,used
R3174 T3655 T3654 arg2Of used,was
R3175 T3657 T3655 arg3Of analyze,used
R3176 T3657 T3656 arg1Of analyze,to
R3177 T3659 T3657 arg2Of intensity,analyze
R3178 T3659 T3658 arg1Of intensity,the
R3179 T3659 T3660 arg1Of intensity,of
R3180 T3662 T3660 arg2Of blots,of
R3181 T3662 T3661 arg1Of blots,the
R3182 T3664 T3663 arg1Of level,The
R3183 T3664 T3665 arg1Of level,of
R3184 T3664 T3667 arg1Of level,was
R3185 T3664 T3668 arg2Of level,used
R3186 T3666 T3665 arg2Of β-actin,of
R3187 T3668 T3667 arg2Of used,was
R3188 T3668 T3669 arg1Of used,as
R3189 T3671 T3669 arg2Of standard,as
R3190 T3671 T3670 arg1Of standard,internal
R3492 T4084 T4082 arg1Of Assay,Enzyme-Linked
R3493 T4084 T4083 arg1Of Assay,Immunosorbent
R3494 T4084 T4085 arg1Of Assay,(
R3495 T4086 T4085 arg2Of ELISA,(
R3496 T4087 T4085 arg3Of ),(
R3497 T4088 T4089 arg1Of Cells,of
R3498 T4088 T4092 arg1Of Cells,were
R3499 T4088 T4093 arg2Of Cells,homogenized
R3500 T4091 T4089 arg2Of groups,of
R3501 T4091 T4090 arg1Of groups,four
R3502 T4093 T4092 arg2Of homogenized,were
R3503 T4093 T4094 arg1Of homogenized,as
R3504 T4095 T4094 arg2Of mentioned,as
R3505 T4095 T4096 arg1Of mentioned,above
R3506 T4098 T4097 arg1Of supernatant,The
R3507 T4098 T4099 arg1Of supernatant,was
R3508 T4098 T4100 arg2Of supernatant,collected
R3509 T4100 T4099 arg2Of collected,was
R3510 T4100 T4102 arg1Of collected,and
R3511 T4102 T4101 arg1Of and,","
R3512 T4104 T4103 arg1Of protein,total
R3513 T4104 T4105 arg1Of protein,was
R3514 T4104 T4106 arg2Of protein,determined
R3515 T4106 T4102 arg2Of determined,and
R3516 T4106 T4105 arg2Of determined,was
R3517 T4112 T4106 arg1Of Reagent,determined
R3518 T4112 T4107 arg2Of Reagent,by
R3519 T4112 T4108 arg1Of Reagent,Coomassie
R3520 T4112 T4109 arg1Of Reagent,Plus
R3521 T4112 T4110 arg1Of Reagent,Protein
R3522 T4112 T4111 arg1Of Reagent,Assay
R3523 T4112 T4113 arg1Of Reagent,(
R3524 T4114 T4115 arg1Of Pierce,","
R3525 T4115 T4113 arg2Of ",",(
R3526 T4115 T4117 arg1Of ",",","
R3527 T4116 T4115 arg2Of IL,","
R3528 T4118 T4117 arg2Of USA,","
R3529 T4119 T4113 arg3Of ),(
R3530 T4120 T4121 arg1Of Levels,of
R3531 T4120 T4135 arg1Of Levels,and
R3532 T4124 T4122 arg1Of factor-alpha,tumor
R3533 T4124 T4123 arg1Of factor-alpha,necrosis
R3534 T4124 T4125 arg1Of factor-alpha,(
R3535 T4124 T4128 arg1Of factor-alpha,","
R3536 T4126 T4125 arg2Of TNF-α,(
R3537 T4127 T4125 arg3Of ),(
R3538 T4128 T4121 arg2Of ",",of
R3539 T4130 T4128 arg2Of beta,","
R3540 T4130 T4129 arg1Of beta,interleukin-1
R3541 T4130 T4131 arg1Of beta,(
R3542 T4132 T4131 arg2Of IL-1β,(
R3543 T4133 T4131 arg3Of ),(
R3544 T4135 T4134 arg1Of and,","
R3545 T4136 T4135 arg2Of interleukin-6,and
R3546 T4136 T4137 arg1Of interleukin-6,(
R3547 T4136 T4141 modOf interleukin-6,were
R3548 T4138 T4137 arg2Of IL-6,(
R3549 T4139 T4137 arg3Of ),(
R3550 T4140 T4141 arg1Of protein,were
R3551 T4140 T4142 arg2Of protein,quantified
R3552 T4142 T4141 arg2Of quantified,were
R3553 T4143 T4142 arg3Of using,quantified
R3554 T4143 T4149 arg1Of using,according
R3555 T4145 T4143 arg2Of kits,using
R3556 T4145 T4144 arg1Of kits,ELISA
R3557 T4145 T4146 arg1Of kits,specific
R3558 T4146 T4147 arg1Of specific,for
R3559 T4148 T4147 arg2Of mouse,for
R3560 T4150 T4149 arg2Of to,according
R3561 T4152 T4151 arg1Of manufacturer,the
R3562 T4152 T4153 arg2Of manufacturer,'s
R3563 T4154 T4150 arg2Of instructions,to
R3564 T4154 T4153 arg1Of instructions,'s
R3565 T4154 T4155 arg1Of instructions,(
R3566 T4159 T4155 arg2Of CA,(
R3567 T4159 T4156 arg1Of CA,Bender
R3568 T4159 T4157 arg1Of CA,MedSystems
R3569 T4159 T4158 arg1Of CA,Inc.
R3570 T4159 T4160 arg1Of CA,","
R3571 T4159 T4161 arg1Of CA,USA
R3572 T4162 T4155 arg3Of ),(
R3573 T4163 T4165 arg1Of Briefly,prepared
R3574 T4163 T4169 arg1Of Briefly,created
R3575 T4165 T4168 arg1Of prepared,and
R3576 T4167 T4165 arg2Of standard,prepared
R3577 T4167 T4166 arg1Of standard,the
R3578 T4168 T4164 arg1Of and,","
R3579 T4169 T4168 arg2Of created,and
R3580 T4172 T4169 arg2Of dilution,created
R3581 T4172 T4170 arg1Of dilution,the
R3582 T4172 T4171 arg1Of dilution,standard
R3583 T4172 T4173 arg1Of dilution,for
R3584 T4174 T4173 arg2Of building,for
R3585 T4176 T4174 arg2Of curve,building
R3586 T4176 T4175 arg1Of curve,standard
R3587 T4178 T4179 arg1Of samples,and
R3588 T4179 T4177 arg1Of and,Then
R3589 T4179 T4181 arg1Of and,were
R3590 T4179 T4182 arg2Of and,added
R3591 T4180 T4179 arg2Of biotinconjugate,and
R3592 T4182 T4181 arg2Of added,were
R3593 T4182 T4183 modOf added,to
R3594 T4184 T4183 arg1Of microwell,to
R3595 T4185 T4184 arg2Of strips,microwell
R3596 T4187 T4188 arg1Of incubated,for
R3597 T4190 T4188 arg2Of h,for
R3598 T4190 T4189 arg1Of h,2
R3599 T4190 T4191 arg1Of h,at
R3600 T4192 T4186 arg1Of room,After
R3601 T4192 T4187 arg2Of room,incubated
R3602 T4193 T4192 arg1Of temperature,room
R3603 T4193 T4194 arg1Of temperature,","
R3604 T4193 T4198 arg1Of temperature,were
R3605 T4193 T4199 arg2Of temperature,washed
R3606 T4197 T4194 arg2Of strips,","
R3607 T4197 T4195 arg1Of strips,the
R3608 T4197 T4196 arg1Of strips,microwell
R3609 T4199 T4198 arg2Of washed,were
R3610 T4199 T4202 arg1Of washed,with
R3611 T4199 T4206 arg1Of washed,and
R3612 T4201 T4199 arg3Of times,washed
R3613 T4201 T4200 arg1Of times,3
R3614 T4204 T4202 arg2Of buffer,with
R3615 T4204 T4203 arg1Of buffer,wash
R3616 T4206 T4205 arg1Of and,","
R3617 T4207 T4208 arg1Of streptavidin-HRP,were
R3618 T4207 T4209 arg2Of streptavidin-HRP,added
R3619 T4209 T4206 arg2Of added,and
R3620 T4209 T4208 arg2Of added,were
R3621 T4209 T4210 arg1Of added,to
R3622 T4212 T4210 arg2Of wells,to
R3623 T4212 T4211 arg1Of wells,all
R3624 T4214 T4215 arg1Of incubated,for
R3625 T4217 T4215 arg2Of h,for
R3626 T4217 T4216 arg1Of h,1
R3627 T4220 T4213 arg1Of strips,After
R3628 T4220 T4214 arg2Of strips,incubated
R3629 T4220 T4218 arg1Of strips,","
R3630 T4220 T4219 arg1Of strips,microwell
R3631 T4220 T4221 arg1Of strips,were
R3632 T4220 T4222 arg2Of strips,washed
R3633 T4222 T4221 arg2Of washed,were
R3634 T4224 T4222 arg3Of times,washed
R3635 T4224 T4223 arg1Of times,3
R3636 T4224 T4225 arg2Of times,followed
R3637 T4225 T4226 arg1Of followed,by
R3638 T4227 T4226 arg2Of adding,by
R3639 T4229 T4227 arg2Of substrate,adding
R3640 T4229 T4228 arg1Of substrate,TMB
R3641 T4231 T4232 arg1Of incubated,for
R3642 T4234 T4230 arg1Of 10–30,After
R3643 T4234 T4231 arg2Of 10–30,incubated
R3644 T4234 T4233 arg1Of 10–30,about
R3645 T4235 T4234 arg1Of min,10–30
R3646 T4235 T4236 arg1Of min,","
R3647 T4235 T4240 arg1Of min,was
R3648 T4235 T4241 arg2Of min,added
R3649 T4239 T4236 arg2Of solution,","
R3650 T4239 T4237 arg1Of solution,the
R3651 T4239 T4238 arg1Of solution,stop
R3652 T4241 T4240 arg2Of added,was
R3653 T4244 T4242 arg1Of intensity,The
R3654 T4244 T4243 arg1Of intensity,colour
R3655 T4244 T4245 arg1Of intensity,was
R3656 T4244 T4246 arg2Of intensity,measured
R3657 T4244 T4250 arg1Of intensity,using
R3658 T4246 T4245 arg2Of measured,was
R3659 T4246 T4247 arg1Of measured,at
R3660 T4246 T4250 modOf measured,using
R3661 T4249 T4247 arg2Of nm,at
R3662 T4249 T4248 arg1Of nm,450
R3663 T4250 T4256 arg1Of using,(
R3664 T4255 T4250 arg2Of reader,using
R3665 T4255 T4251 arg1Of reader,a
R3666 T4255 T4252 arg1Of reader,Bio-Rad
R3667 T4255 T4253 arg1Of reader,ELISA
R3668 T4255 T4254 arg1Of reader,microplate
R3669 T4258 T4256 arg2Of Laboratories,(
R3670 T4258 T4257 arg1Of Laboratories,Bio-Rad
R3671 T4258 T4259 arg1Of Laboratories,","
R3672 T4260 T4259 arg2Of CA,","
R3673 T4260 T4261 arg1Of CA,","
R3674 T4262 T4261 arg2Of USA,","
R3675 T4263 T4256 arg3Of ),(
R3676 T4265 T4264 arg1Of concentration,The
R3677 T4265 T4266 arg1Of concentration,of
R3678 T4265 T4268 arg1Of concentration,was
R3679 T4265 T4269 arg2Of concentration,determined
R3680 T4265 T4276 arg2Of concentration,expressed
R3681 T4267 T4266 arg2Of protein,of
R3682 T4269 T4270 arg1Of determined,according
R3683 T4269 T4275 arg1Of determined,and
R3684 T4271 T4270 arg2Of to,according
R3685 T4274 T4271 arg2Of curve,to
R3686 T4274 T4272 arg1Of curve,the
R3687 T4274 T4273 arg1Of curve,standard
R3688 T4275 T4268 arg2Of and,was
R3689 T4276 T4275 arg2Of expressed,and
R3690 T4276 T4277 arg1Of expressed,as
R3691 T4278 T4277 arg2Of pg/mg,as
R3692 T4278 T4279 arg1Of pg/mg,of
R3693 T4281 T4279 arg2Of protein,of
R3694 T4281 T4280 arg1Of protein,total
R3709 T4394 T4393 arg1Of Zymography,Gelatine
R3710 T4395 T4396 arg1Of Cells,of
R3711 T4395 T4399 arg1Of Cells,were
R3712 T4395 T4400 arg2Of Cells,homogenized
R3713 T4398 T4396 arg2Of groups,of
R3714 T4398 T4397 arg1Of groups,four
R3715 T4400 T4399 arg2Of homogenized,were
R3716 T4400 T4401 arg1Of homogenized,in
R3717 T4400 T4425 arg1Of homogenized,and
R3718 T4403 T4401 arg2Of buffer,in
R3719 T4403 T4402 arg1Of buffer,lysis
R3720 T4403 T4404 arg1Of buffer,containing
R3721 T4405 T4406 arg1Of 50,mM
R3722 T4407 T4405 arg1Of Tris-HCl,50
R3723 T4407 T4408 arg1Of Tris-HCl,(
R3724 T4407 T4412 arg1Of Tris-HCl,","
R3725 T4409 T4408 arg2Of pH,(
R3726 T4409 T4410 arg1Of pH,7.4
R3727 T4411 T4408 arg3Of ),(
R3728 T4412 T4416 arg1Of ",",","
R3729 T4413 T4414 arg1Of 150,mM
R3730 T4415 T4412 arg2Of NaCl,","
R3731 T4415 T4413 arg1Of NaCl,150
R3732 T4416 T4420 arg1Of ",",","
R3733 T4417 T4418 arg1Of 5,mM
R3734 T4419 T4416 arg2Of CaCl2,","
R3735 T4419 T4417 arg1Of CaCl2,5
R3736 T4420 T4404 arg2Of ",",containing
R3737 T4421 T4422 arg1Of 0.2,mM
R3738 T4423 T4420 arg2Of NaN3,","
R3739 T4423 T4421 arg1Of NaN3,0.2
R3740 T4425 T4424 arg1Of and,","
R3741 T4427 T4426 arg1Of %,0.01
R3742 T4429 T4428 arg1Of Soluble,Triton.
R3743 T4430 T4429 arg1Of extracts,Soluble
R3744 T4430 T4431 arg1Of extracts,were
R3745 T4430 T4432 arg2Of extracts,separated
R3746 T4430 T4436 arg2Of extracts,stored
R3747 T4432 T4435 arg1Of separated,and
R3748 T4434 T4432 arg1Of centrifugation,separated
R3749 T4434 T4433 arg2Of centrifugation,by
R3750 T4435 T4425 arg2Of and,and
R3751 T4435 T4427 arg1Of and,%
R3752 T4435 T4431 arg2Of and,were
R3753 T4436 T4435 arg2Of stored,and
R3754 T4436 T4437 arg1Of stored,at
R3755 T4438 T4437 arg2Of –20°C,at
R3756 T4440 T4439 arg1Of zymography,Gelatin
R3757 T4440 T4441 arg1Of zymography,was
R3758 T4440 T4442 arg2Of zymography,performed
R3759 T4442 T4441 arg2Of performed,was
R3760 T4442 T4443 arg1Of performed,according
R3761 T4444 T4443 arg2Of to,according
R3762 T4446 T4445 arg1Of manufacturer,the
R3763 T4446 T4447 arg2Of manufacturer,'s
R3764 T4448 T4444 arg2Of instructions,to
R3765 T4448 T4447 arg1Of instructions,'s
R3766 T4448 T4449 arg1Of instructions,(
R3767 T4452 T4449 arg2Of Inc,(
R3768 T4452 T4450 arg1Of Inc,Genmed
R3769 T4452 T4451 arg1Of Inc,Scientifics
R3770 T4452 T4453 arg1Of Inc,","
R3771 T4454 T4453 arg2Of MA,","
R3772 T4454 T4455 arg1Of MA,","
R3773 T4456 T4455 arg2Of USA,","
R3774 T4457 T4449 arg3Of ),(
R3775 T4464 T4460 arg1Of extracts,40
R3776 T4464 T4461 arg1Of extracts,μg
R3777 T4464 T4462 arg1Of extracts,cytosolic
R3778 T4464 T4463 arg1Of extracts,protein
R3779 T4464 T4465 arg1Of extracts,were
R3780 T4464 T4466 arg2Of extracts,separated
R3781 T4466 T4458 arg1Of separated,Briefly
R3782 T4466 T4459 arg1Of separated,","
R3783 T4466 T4465 arg2Of separated,were
R3784 T4468 T4466 arg1Of electrophoresis,separated
R3785 T4468 T4467 arg2Of electrophoresis,by
R3786 T4471 T4470 arg1Of proteins,the
R3787 T4471 T4472 arg1Of proteins,were
R3788 T4471 T4473 arg2Of proteins,renatured
R3789 T4471 T4483 arg2Of proteins,incubated
R3790 T4473 T4474 arg1Of renatured,by
R3791 T4473 T4481 arg1Of renatured,and
R3792 T4475 T4474 arg2Of incubation,by
R3793 T4475 T4476 arg1Of incubation,in
R3794 T4477 T4478 arg1Of 2.5,%
R3795 T4480 T4476 arg2Of X-100,in
R3796 T4480 T4477 arg1Of X-100,2.5
R3797 T4480 T4479 arg1Of X-100,Triton
R3798 T4481 T4469 arg1Of and,Then
R3799 T4481 T4472 arg2Of and,were
R3800 T4483 T4481 arg2Of incubated,and
R3801 T4483 T4482 arg1Of incubated,then
R3802 T4483 T4484 arg1Of incubated,in
R3803 T4483 T4487 arg1Of incubated,for
R3804 T4483 T4490 arg1Of incubated,at
R3805 T4483 T4492 modOf incubated,to
R3806 T4486 T4484 arg2Of buffer,in
R3807 T4486 T4485 arg1Of buffer,substrate
R3808 T4489 T4487 arg2Of h,for
R3809 T4489 T4488 arg1Of h,40
R3810 T4491 T4490 arg2Of 37°C,at
R3811 T4493 T4492 arg1Of enable,to
R3812 T4495 T4493 arg2Of MMP9,enable
R3813 T4495 T4494 arg1Of MMP9,the
R3814 T4495 T4497 arg1Of MMP9,cleave
R3815 T4497 T4493 arg3Of cleave,enable
R3816 T4497 T4496 arg1Of cleave,to
R3817 T4499 T4497 arg2Of gelatin,cleave
R3818 T4499 T4498 arg1Of gelatin,the
R3819 T4501 T4500 arg2Of rinsing,After
R3820 T4501 T4502 arg1Of rinsing,in
R3821 T4503 T4502 arg2Of water,in
R3822 T4506 T4501 arg1Of gel,rinsing
R3823 T4506 T4505 arg1Of gel,each
R3824 T4506 T4507 arg1Of gel,was
R3825 T4506 T4508 arg2Of gel,stained
R3826 T4506 T4511 arg1Of gel,blue
R3827 T4506 T4516 arg2Of gel,destained
R3828 T4508 T4509 arg1Of stained,with
R3829 T4508 T4515 arg1Of stained,and
R3830 T4510 T4509 arg2Of Coomassie,with
R3831 T4511 T4508 arg3Of blue,stained
R3832 T4511 T4512 arg1Of blue,for
R3833 T4514 T4512 arg2Of h,for
R3834 T4514 T4513 arg1Of h,1
R3835 T4515 T4500 arg1Of and,After
R3836 T4515 T4504 arg1Of and,","
R3837 T4515 T4507 arg2Of and,was
R3838 T4516 T4515 arg2Of destained,and
R3839 T4516 T4517 arg1Of destained,in
R3840 T4518 T4519 arg1Of 50,%
R3841 T4520 T4517 arg2Of methanol,in
R3842 T4520 T4518 arg1Of methanol,50
R3843 T4522 T4521 arg1Of activities,Proteolytic
R3844 T4522 T4523 arg1Of activities,were
R3845 T4522 T4524 arg2Of activities,showed
R3846 T4524 T4523 arg2Of showed,were
R3847 T4527 T4524 arg1Of bands,showed
R3848 T4527 T4525 arg2Of bands,by
R3849 T4527 T4526 arg1Of bands,clear
R3850 T4527 T4528 arg1Of bands,in
R3851 T4530 T4528 arg2Of gel,in
R3852 T4530 T4529 arg1Of gel,blue
R3853 T4530 T4531 arg1Of gel,which
R3854 T4530 T4532 arg1Of gel,indicates
R3855 T4534 T4532 arg2Of lysis,indicates
R3856 T4534 T4533 arg1Of lysis,the
R3857 T4534 T4535 arg1Of lysis,of
R3858 T4537 T4535 arg2Of substrate,of
R3859 T4537 T4536 arg1Of substrate,the
R3860 T4538 T4539 arg1Of Quantification,of
R3861 T4538 T4543 arg1Of Quantification,was
R3862 T4538 T4544 arg2Of Quantification,performed
R3863 T4542 T4539 arg2Of density,of
R3864 T4542 T4540 arg1Of density,MMP9
R3865 T4542 T4541 arg1Of density,band
R3866 T4544 T4543 arg2Of performed,was
R3867 T4544 T4545 arg1Of performed,with
R3868 T4549 T4545 arg2Of ImageJ,with
R3869 T4549 T4546 arg1Of ImageJ,image
R3870 T4549 T4547 arg1Of ImageJ,analysis
R3871 T4549 T4548 arg1Of ImageJ,program
R4043 T4764 T4763 arg1Of Analysis,Statistical
R4044 T4765 T4766 arg1Of Data,were
R4045 T4765 T4767 arg2Of Data,expressed
R4046 T4765 T4773 arg2Of Data,evaluated
R4047 T4767 T4768 arg1Of expressed,as
R4048 T4767 T4772 arg1Of expressed,and
R4049 T4771 T4768 arg2Of SD,as
R4050 T4771 T4769 arg1Of SD,mean
R4051 T4771 T4770 arg1Of SD,±
R4052 T4772 T4766 arg2Of and,were
R4053 T4773 T4772 arg2Of evaluated,and
R4054 T4775 T4776 arg1Of ANOVA,and
R4055 T4777 T4776 arg2Of LSD,and
R4056 T4780 T4773 arg1Of test,evaluated
R4057 T4780 T4774 arg2Of test,by
R4058 T4780 T4775 arg1Of test,ANOVA
R4059 T4780 T4777 arg1Of test,LSD
R4060 T4780 T4778 arg1Of test,multiple
R4061 T4780 T4779 arg1Of test,comparison
R4062 T4782 T4781 arg1Of values,P
R4063 T4782 T4783 arg1Of values,<
R4064 T4782 T4784 arg1Of values,0.05
R4065 T4782 T4785 arg1Of values,were
R4066 T4782 T4786 arg2Of values,considered
R4067 T4782 T4788 arg1Of values,be
R4068 T4782 T4789 arg1Of values,significant
R4069 T4786 T4785 arg2Of considered,were
R4070 T4788 T4786 arg3Of be,considered
R4071 T4788 T4787 arg1Of be,to
R4072 T4789 T4788 arg2Of significant,be
R4073 T4791 T4790 arg1Of analyses,All
R4074 T4791 T4792 arg1Of analyses,were
R4075 T4791 T4793 arg2Of analyses,performed
R4076 T4793 T4792 arg2Of performed,were
R4077 T4793 T4794 arg1Of performed,by
R4078 T4795 T4794 arg2Of using,by
R4079 T4798 T4795 arg2Of software,using
R4080 T4798 T4796 arg1Of software,SPSS
R4081 T4798 T4797 arg1Of software,18.0
R4123 T4857 T4858 arg1Of Depletion,of
R4124 T4857 T4860 arg1Of Depletion,Aggravated
R4125 T4859 T4858 arg2Of Nrf2,of
R4126 T4860 T4866 arg1Of Aggravated,after
R4127 T4863 T4860 arg2Of Death,Aggravated
R4128 T4863 T4861 arg1Of Death,the
R4129 T4863 T4862 arg1Of Death,Cell
R4130 T4863 T4864 arg1Of Death,in
R4131 T4865 T4864 arg2Of Astrocytes,in
R4132 T4868 T4866 arg2Of Injury,after
R4133 T4868 T4867 arg1Of Injury,Scratch
R4134 T4870 T4869 arg1Of death,Cell
R4135 T4870 T4871 arg1Of death,was
R4136 T4870 T4872 arg2Of death,detected
R4137 T4872 T4871 arg2Of detected,was
R4138 T4874 T4875 arg1Of microscope,and
R4139 T4876 T4875 arg2Of CCK-8,and
R4140 T4877 T4872 arg1Of analysis,detected
R4141 T4877 T4873 arg2Of analysis,by
R4142 T4877 T4874 arg1Of analysis,microscope
R4143 T4877 T4876 arg1Of analysis,CCK-8
R4144 T4877 T4878 arg1Of analysis,at
R4145 T4880 T4878 arg2Of h,at
R4146 T4880 T4879 arg1Of h,24
R4147 T4880 T4881 arg1Of h,after
R4148 T4882 T4881 arg2Of scratch,after
R4149 T4884 T4883 arg1Of detachment,The
R4150 T4884 T4885 arg1Of detachment,from
R4151 T4884 T4896 arg1Of detachment,were
R4152 T4884 T4898 arg2Of detachment,observed
R4153 T4887 T4886 arg1Of plate,culture
R4154 T4887 T4888 arg1Of plate,","
R4155 T4888 T4892 arg1Of ",",and
R4156 T4890 T4888 arg2Of lost,","
R4157 T4890 T4889 arg1Of lost,cell
R4158 T4892 T4885 arg2Of and,from
R4159 T4892 T4891 arg1Of and,","
R4160 T4895 T4892 arg2Of distortion,and
R4161 T4895 T4893 arg1Of distortion,cytoplasmic
R4162 T4895 T4894 arg1Of distortion,process
R4163 T4898 T4896 arg2Of observed,were
R4164 T4898 T4897 arg1Of observed,mostly
R4165 T4898 T4899 arg1Of observed,in
R4166 T4901 T4899 arg2Of sides,in
R4167 T4901 T4900 arg1Of sides,both
R4168 T4901 T4902 arg1Of sides,of
R4169 T4901 T4905 arg1Of sides,in
R4170 T4904 T4902 arg2Of line,of
R4171 T4904 T4903 arg1Of line,scratch
R4172 T4908 T4905 arg2Of TBI,in
R4173 T4908 T4906 arg1Of TBI,group
R4174 T4908 T4907 arg1Of TBI,KO
R4175 T4910 T4909 arg1Of assay,CCK-8
R4176 T4910 T4912 arg1Of assay,showed
R4177 T4912 T4911 arg1Of showed,also
R4178 T4915 T4912 arg2Of value,showed
R4179 T4915 T4913 arg1Of value,lower
R4180 T4915 T4914 arg1Of value,OD
R4181 T4915 T4916 arg1Of value,of
R4182 T4919 T4916 arg2Of TBI,of
R4183 T4919 T4917 arg1Of TBI,group
R4184 T4919 T4918 arg1Of TBI,KO
R4185 T4919 T4920 arg1Of TBI,(
R4186 T4919 T4925 arg1Of TBI,than
R4187 T4921 T4922 arg1Of 0.38,±
R4188 T4922 T4920 arg2Of ±,(
R4189 T4923 T4922 arg2Of 0.064,±
R4190 T4924 T4920 arg3Of ),(
R4191 T4926 T4925 arg2Of that,than
R4192 T4926 T4927 arg1Of that,of
R4193 T4930 T4927 arg2Of TBI,of
R4194 T4930 T4928 arg1Of TBI,group
R4195 T4930 T4929 arg1Of TBI,WT
R4196 T4930 T4931 arg1Of TBI,(
R4197 T4930 T4936 arg1Of TBI,(
R4198 T4930 T4941 arg1Of TBI,","
R4199 T4930 T4942 arg1Of TBI,which
R4200 T4930 T4944 arg1Of TBI,suggested
R4201 T4932 T4933 arg1Of 0.98,±
R4202 T4933 T4931 arg2Of ±,(
R4203 T4934 T4933 arg2Of 0.098,±
R4204 T4935 T4931 arg3Of ),(
R4205 T4937 T4936 arg2Of P,(
R4206 T4937 T4938 arg1Of P,<
R4207 T4937 T4939 arg1Of P,0.01
R4208 T4940 T4936 arg3Of ),(
R4209 T4944 T4943 arg1Of suggested,also
R4210 T4947 T4944 arg2Of death,suggested
R4211 T4947 T4945 arg1Of death,more
R4212 T4947 T4946 arg1Of death,cell
R4213 T4947 T4948 arg1Of death,in
R4214 T4951 T4948 arg2Of TBI,in
R4215 T4951 T4949 arg1Of TBI,group
R4216 T4951 T4950 arg1Of TBI,KO
R4217 T4951 T4952 arg1Of TBI,(
R4218 T4953 T4952 arg2Of Figure,(
R4219 T4953 T4954 arg1Of Figure,1
R4220 T4955 T4952 arg3Of ),(
R4221 T4956 T4957 arg1Of There,was
R4222 T4959 T4957 arg2Of difference,was
R4223 T4959 T4958 arg1Of difference,no
R4224 T4959 T4960 arg1Of difference,between
R4225 T4963 T4960 arg2Of groups,between
R4226 T4963 T4961 arg1Of groups,two
R4227 T4963 T4962 arg1Of groups,sham-operated
R4344 T5114 T5115 arg1Of Disruption,of
R4345 T5114 T5117 arg1Of Disruption,Enhanced
R4346 T5116 T5115 arg2Of Nrf2,of
R4347 T5117 T5123 arg1Of Enhanced,after
R4348 T5118 T5117 arg2Of Upregulation,Enhanced
R4349 T5118 T5119 arg1Of Upregulation,of
R4350 T5122 T5119 arg2Of Activity,of
R4351 T5122 T5120 arg1Of Activity,NF-κB
R4352 T5122 T5121 arg1Of Activity,DNA-Binding
R4353 T5125 T5123 arg2Of Injury,after
R4354 T5125 T5124 arg1Of Injury,Scratch
R4355 T5126 T5127 arg1Of It,has
R4356 T5126 T5128 arg1Of It,been
R4357 T5126 T5129 arg2Of It,reported
R4358 T5129 T5127 arg2Of reported,has
R4359 T5129 T5128 arg2Of reported,been
R4360 T5131 T5132 arg1Of NF-κB,is
R4361 T5131 T5133 arg2Of NF-κB,activated
R4362 T5133 T5129 arg3Of activated,reported
R4363 T5133 T5130 arg1Of activated,that
R4364 T5133 T5132 arg2Of activated,is
R4365 T5133 T5134 arg1Of activated,in
R4366 T5135 T5134 arg2Of brain,in
R4367 T5135 T5136 arg1Of brain,after
R4368 T5137 T5136 arg2Of TBI,after
R4369 T5137 T5138 arg1Of TBI,[
R4370 T5139 T5138 arg2Of 2,[
R4371 T5140 T5138 arg3Of ],[
R4372 T5143 T5144 arg1Of we,studied
R4373 T5144 T5141 arg1Of studied,Here
R4374 T5144 T5142 arg1Of studied,","
R4375 T5144 T5158 arg1Of studied,by
R4376 T5147 T5144 arg2Of activity,studied
R4377 T5147 T5145 arg1Of activity,the
R4378 T5147 T5146 arg1Of activity,DNA-binding
R4379 T5147 T5148 arg1Of activity,of
R4380 T5147 T5150 arg1Of activity,of
R4381 T5149 T5148 arg2Of NF-κB,of
R4382 T5151 T5150 arg2Of astrocytes,of
R4383 T5151 T5152 arg1Of astrocytes,from
R4384 T5154 T5153 arg1Of WT,Nrf2
R4385 T5154 T5155 arg1Of WT,or
R4386 T5155 T5152 arg2Of or,from
R4387 T5157 T5155 arg2Of mice,or
R4388 T5157 T5156 arg1Of mice,KO
R4389 T5159 T5158 arg2Of EMSA,by
R4390 T5159 T5160 arg1Of EMSA,at
R4391 T5162 T5160 arg2Of h,at
R4392 T5162 T5161 arg1Of h,24
R4393 T5162 T5163 arg1Of h,after
R4394 T5165 T5163 arg2Of injury,after
R4395 T5165 T5164 arg1Of injury,scratch
R4396 T5167 T5166 arg1Of injury,Scratch
R4397 T5167 T5168 arg1Of injury,induced
R4398 T5168 T5177 arg1Of induced,","
R4399 T5168 T5178 arg1Of induced,while
R4400 T5168 T5193 arg1Of induced,(
R4401 T5168 T5206 arg1Of induced,(
R4402 T5169 T5168 arg2Of activation,induced
R4403 T5169 T5170 arg1Of activation,of
R4404 T5169 T5172 arg1Of activation,in
R4405 T5171 T5170 arg2Of NF-κB,of
R4406 T5173 T5172 arg2Of astrocytes,in
R4407 T5173 T5174 arg1Of astrocytes,of
R4408 T5176 T5174 arg2Of genotypes,of
R4409 T5176 T5175 arg1Of genotypes,both
R4410 T5181 T5179 arg1Of activity,higher
R4411 T5181 T5180 arg1Of activity,NF-κB
R4412 T5181 T5182 arg1Of activity,was
R4413 T5181 T5183 arg2Of activity,observed
R4414 T5183 T5178 arg2Of observed,while
R4415 T5183 T5182 arg2Of observed,was
R4416 T5183 T5184 arg1Of observed,in
R4417 T5183 T5188 arg1Of observed,than
R4418 T5187 T5184 arg2Of TBI,in
R4419 T5187 T5185 arg1Of TBI,group
R4420 T5187 T5186 arg1Of TBI,KO
R4421 T5189 T5188 arg2Of in,than
R4422 T5192 T5189 arg2Of TBI,in
R4423 T5192 T5190 arg1Of TBI,group
R4424 T5192 T5191 arg1Of TBI,WT
R4425 T5196 T5197 arg1Of 0.173,versus
R4426 T5198 T5197 arg2Of 2.28,versus
R4427 T5198 T5199 arg1Of 2.28,±
R4428 T5200 T5193 arg2Of 0.072,(
R4429 T5200 T5194 arg1Of 0.072,2.67
R4430 T5200 T5195 arg1Of 0.072,±
R4431 T5200 T5196 arg1Of 0.072,0.173
R4432 T5200 T5201 arg1Of 0.072,","
R4433 T5202 T5201 arg2Of P,","
R4434 T5202 T5203 arg1Of P,<
R4435 T5202 T5204 arg1Of P,0.01
R4436 T5205 T5193 arg3Of ),(
R4437 T5207 T5206 arg2Of Figure,(
R4438 T5207 T5208 arg1Of Figure,2
R4439 T5209 T5206 arg3Of ),(
R4574 T5469 T5470 arg1Of Expression,of
R4575 T5469 T5473 arg1Of Expression,Was
R4576 T5469 T5474 arg2Of Expression,Elevated
R4577 T5472 T5470 arg2Of Cytokines,of
R4578 T5472 T5471 arg1Of Cytokines,Proinflammatory
R4579 T5474 T5473 arg2Of Elevated,Was
R4580 T5474 T5475 arg1Of Elevated,in
R4581 T5474 T5479 arg1Of Elevated,after
R4582 T5478 T5475 arg2Of Astrocytes,in
R4583 T5478 T5476 arg1Of Astrocytes,Nrf2
R4584 T5478 T5477 arg1Of Astrocytes,Knockout
R4585 T5481 T5479 arg2Of Injury,after
R4586 T5481 T5480 arg1Of Injury,Scratch
R4587 T5484 T5483 arg1Of all,we
R4588 T5484 T5485 arg1Of all,know
R4589 T5485 T5482 arg2Of know,As
R4590 T5487 T5488 arg1Of TNF-α,","
R4591 T5488 T5491 arg1Of ",",and
R4592 T5489 T5488 arg2Of IL-1β,","
R4593 T5491 T5490 arg1Of and,","
R4594 T5491 T5493 arg1Of and,are
R4595 T5491 T5494 arg2Of and,regulated
R4596 T5491 T5498 arg1Of and,reflect
R4597 T5492 T5491 arg2Of IL-6,and
R4598 T5494 T5493 arg2Of regulated,are
R4599 T5494 T5497 arg1Of regulated,and
R4600 T5496 T5494 arg1Of NF-κB,regulated
R4601 T5496 T5495 arg2Of NF-κB,by
R4602 T5497 T5482 arg1Of and,As
R4603 T5497 T5486 arg1Of and,","
R4604 T5498 T5497 arg2Of reflect,and
R4605 T5501 T5498 arg2Of activity,reflect
R4606 T5501 T5499 arg1Of activity,the
R4607 T5501 T5500 arg1Of activity,endogenous
R4608 T5501 T5502 arg1Of activity,of
R4609 T5503 T5502 arg2Of NF-κB,of
R4610 T5504 T5505 arg1Of It,has
R4611 T5504 T5506 arg1Of It,been
R4612 T5504 T5507 arg2Of It,revealed
R4613 T5507 T5505 arg2Of revealed,has
R4614 T5507 T5506 arg2Of revealed,been
R4615 T5511 T5509 arg1Of cytokines,these
R4616 T5511 T5510 arg1Of cytokines,proinflammatory
R4617 T5511 T5512 arg1Of cytokines,are
R4618 T5511 T5513 arg2Of cytokines,increased
R4619 T5513 T5507 arg3Of increased,revealed
R4620 T5513 T5508 arg1Of increased,that
R4621 T5513 T5512 arg2Of increased,are
R4622 T5513 T5514 arg1Of increased,after
R4623 T5515 T5514 arg2Of TBI,after
R4624 T5515 T5516 arg1Of TBI,in
R4625 T5517 T5516 arg2Of animals,in
R4626 T5517 T5518 arg1Of animals,[
R4627 T5519 T5518 arg2Of 2,[
R4628 T5519 T5520 arg1Of 2,","
R4629 T5521 T5520 arg2Of 8,","
R4630 T5522 T5518 arg3Of ],[
R4631 T5526 T5524 arg1Of study,our
R4632 T5526 T5525 arg1Of study,previous
R4633 T5526 T5527 arg1Of study,has
R4634 T5526 T5528 arg1Of study,proved
R4635 T5528 T5523 arg1Of proved,And
R4636 T5528 T5527 arg2Of proved,has
R4637 T5531 T5530 arg1Of levels,higher
R4638 T5531 T5532 arg1Of levels,of
R4639 T5531 T5536 arg1Of levels,were
R4640 T5531 T5537 arg2Of levels,observed
R4641 T5535 T5532 arg2Of cytokines,of
R4642 T5535 T5533 arg1Of cytokines,such
R4643 T5535 T5534 arg1Of cytokines,proinflammatory
R4644 T5537 T5528 arg2Of observed,proved
R4645 T5537 T5529 arg1Of observed,that
R4646 T5537 T5536 arg2Of observed,were
R4647 T5537 T5538 arg1Of observed,in
R4648 T5540 T5538 arg2Of brain,in
R4649 T5540 T5539 arg1Of brain,the
R4650 T5540 T5541 arg1Of brain,of
R4651 T5540 T5544 arg1Of brain,after
R4652 T5543 T5541 arg2Of mice,of
R4653 T5543 T5542 arg1Of mice,Nrf2-deficient
R4654 T5545 T5544 arg2Of TBI,after
R4655 T5545 T5546 arg1Of TBI,[
R4656 T5547 T5546 arg2Of 5,[
R4657 T5548 T5546 arg3Of ],[
R4658 T5550 T5551 arg1Of it,is
R4659 T5550 T5553 arg1Of it,ambiguous
R4660 T5551 T5549 arg1Of is,But
R4661 T5551 T5552 arg1Of is,still
R4662 T5553 T5551 arg2Of ambiguous,is
R4663 T5553 T5554 arg1Of ambiguous,about
R4664 T5556 T5554 arg2Of relationship,about
R4665 T5556 T5555 arg1Of relationship,the
R4666 T5556 T5557 arg1Of relationship,between
R4667 T5559 T5560 arg1Of cytokines,and
R4668 T5560 T5557 arg2Of and,between
R4669 T5560 T5558 arg1Of and,these
R4670 T5560 T5562 arg1Of and,in
R4671 T5561 T5560 arg2Of Nrf2,and
R4672 T5563 T5562 arg2Of astrocytes,in
R4673 T5563 T5564 arg1Of astrocytes,after
R4674 T5565 T5564 arg2Of TBI,after
R4675 T5568 T5569 arg1Of we,tested
R4676 T5569 T5566 arg1Of tested,Here
R4677 T5569 T5567 arg1Of tested,","
R4678 T5569 T5582 arg1Of tested,at
R4679 T5571 T5569 arg2Of levels,tested
R4680 T5571 T5570 arg1Of levels,the
R4681 T5571 T5572 arg1Of levels,of
R4682 T5573 T5574 arg1Of TNF-α,","
R4683 T5574 T5577 arg1Of ",",and
R4684 T5575 T5574 arg2Of IL-1β,","
R4685 T5577 T5572 arg2Of and,of
R4686 T5577 T5576 arg1Of and,","
R4687 T5577 T5579 arg1Of and,in
R4688 T5578 T5577 arg2Of IL-6,and
R4689 T5581 T5579 arg2Of astrocytes,in
R4690 T5581 T5580 arg2Of astrocytes,cultured
R4691 T5584 T5582 arg2Of h,at
R4692 T5584 T5583 arg1Of h,24
R4693 T5584 T5585 arg1Of h,after
R4694 T5587 T5585 arg2Of injury,after
R4695 T5587 T5586 arg1Of injury,scratch
R4696 T5587 T5588 arg1Of injury,by
R4697 T5589 T5590 arg1Of RT-PCR,and
R4698 T5590 T5588 arg2Of and,by
R4699 T5591 T5590 arg2Of ELISA,and
R4700 T5592 T5593 arg1Of It,was
R4701 T5592 T5594 arg2Of It,shown
R4702 T5594 T5593 arg2Of shown,was
R4703 T5594 T5622 arg1Of shown,","
R4704 T5594 T5623 arg1Of shown,respectively
R4705 T5598 T5596 arg1Of levels,the
R4706 T5598 T5597 arg1Of levels,mRNA
R4707 T5598 T5599 arg1Of levels,of
R4708 T5598 T5606 arg1Of levels,were
R4709 T5598 T5607 arg2Of levels,increased
R4710 T5598 T5617 arg1Of levels,compared
R4711 T5600 T5601 arg1Of TNF-α,","
R4712 T5601 T5604 arg1Of ",",and
R4713 T5602 T5601 arg2Of IL-1β,","
R4714 T5604 T5599 arg2Of and,of
R4715 T5604 T5603 arg1Of and,","
R4716 T5605 T5604 arg2Of IL-6,and
R4717 T5607 T5594 arg3Of increased,shown
R4718 T5607 T5595 arg1Of increased,that
R4719 T5607 T5606 arg2Of increased,were
R4720 T5607 T5608 arg1Of increased,after
R4721 T5607 T5610 arg1Of increased,in
R4722 T5607 T5616 arg1Of increased,when
R4723 T5609 T5608 arg2Of scratch,after
R4724 T5612 T5613 arg1Of WT,and
R4725 T5614 T5613 arg2Of KO,and
R4726 T5615 T5610 arg2Of astrocytes,in
R4727 T5615 T5611 arg1Of astrocytes,both
R4728 T5615 T5612 arg1Of astrocytes,WT
R4729 T5615 T5614 arg1Of astrocytes,KO
R4730 T5617 T5616 arg2Of compared,when
R4731 T5617 T5618 arg1Of compared,with
R4732 T5621 T5618 arg2Of counterparts,with
R4733 T5621 T5619 arg1Of counterparts,their
R4734 T5621 T5620 arg1Of counterparts,sham
R4735 T5628 T5626 arg1Of levels,the
R4736 T5628 T5627 arg1Of levels,mRNA
R4737 T5628 T5629 arg1Of levels,of
R4738 T5628 T5636 arg1Of levels,were
R4739 T5628 T5638 arg1Of levels,higher
R4740 T5631 T5629 arg2Of cytokines,of
R4741 T5631 T5630 arg1Of cytokines,these
R4742 T5631 T5632 arg1Of cytokines,in
R4743 T5635 T5632 arg2Of TBI,in
R4744 T5635 T5633 arg1Of TBI,group
R4745 T5635 T5634 arg1Of TBI,KO
R4746 T5636 T5624 arg1Of were,Moreover
R4747 T5636 T5625 arg1Of were,","
R4748 T5636 T5652 arg1Of were,0.110
R4749 T5638 T5636 arg2Of higher,were
R4750 T5638 T5637 arg1Of higher,much
R4751 T5638 T5639 arg1Of higher,than
R4752 T5638 T5648 arg1Of higher,0.085
R4753 T5640 T5639 arg2Of those,than
R4754 T5640 T5641 arg1Of those,in
R4755 T5644 T5641 arg2Of TBI,in
R4756 T5644 T5642 arg1Of TBI,group
R4757 T5644 T5643 arg1Of TBI,WT
R4758 T5648 T5645 arg1Of 0.085,(
R4759 T5648 T5646 arg1Of 0.085,1.66
R4760 T5648 T5647 arg1Of 0.085,±
R4761 T5648 T5649 arg1Of 0.085,versus
R4762 T5650 T5649 arg2Of 1.39,versus
R4763 T5650 T5651 arg1Of 1.39,±
R4764 T5652 T5653 arg1Of 0.110,for
R4765 T5654 T5655 arg1Of TNF-α,","
R4766 T5654 T5666 arg1Of TNF-α,and
R4767 T5658 T5659 arg1Of 0.064,versus
R4768 T5658 T5661 arg1Of 0.064,±
R4769 T5660 T5659 arg2Of 0.73,versus
R4770 T5662 T5655 arg2Of 0.088,","
R4771 T5662 T5656 arg1Of 0.088,1.34
R4772 T5662 T5657 arg1Of 0.088,±
R4773 T5662 T5658 arg1Of 0.088,0.064
R4774 T5662 T5663 arg1Of 0.088,for
R4775 T5664 T5663 arg2Of IL-1β,for
R4776 T5666 T5653 arg2Of and,for
R4777 T5666 T5665 arg1Of and,","
R4778 T5669 T5670 arg1Of 0.102,versus
R4779 T5669 T5672 arg1Of 0.102,±
R4780 T5671 T5670 arg2Of 0.99,versus
R4781 T5673 T5666 arg2Of 0.073,and
R4782 T5673 T5667 arg1Of 0.073,1.28
R4783 T5673 T5668 arg1Of 0.073,±
R4784 T5673 T5669 arg1Of 0.073,0.102
R4785 T5673 T5674 arg1Of 0.073,for
R4786 T5675 T5674 arg2Of IL-6,for
R4787 T5676 T5677 arg1Of P,<
R4788 T5676 T5678 arg1Of P,0.01
R4789 T5678 T5679 arg1Of 0.01,)
R4790 T5678 T5680 arg1Of 0.01,(
R4791 T5682 T5680 arg2Of 3,(
R4792 T5682 T5681 arg1Of 3,Figures
R4793 T5682 T5683 arg1Of 3,(
R4794 T5688 T5683 arg2Of d,(
R4795 T5688 T5684 arg1Of d,a
R4796 T5688 T5685 arg1Of d,)
R4797 T5688 T5686 arg1Of d,–3
R4798 T5688 T5687 arg1Of d,(
R4799 T5689 T5683 arg3Of ),(
R4800 T5690 T5680 arg3Of ),(
R4801 T5692 T5691 arg1Of results,The
R4802 T5692 T5693 arg1Of results,of
R4803 T5692 T5695 arg1Of results,revealed
R4804 T5694 T5693 arg2Of ELISA,of
R4805 T5699 T5697 arg1Of level,the
R4806 T5699 T5698 arg1Of level,protein
R4807 T5699 T5700 arg1Of level,of
R4808 T5699 T5702 arg1Of level,was
R4809 T5699 T5703 arg2Of level,upregulated
R4810 T5699 T5713 arg1Of level,compared
R4811 T5701 T5700 arg2Of TNF-α,of
R4812 T5703 T5695 arg2Of upregulated,revealed
R4813 T5703 T5696 arg1Of upregulated,that
R4814 T5703 T5702 arg2Of upregulated,was
R4815 T5703 T5704 arg1Of upregulated,after
R4816 T5703 T5712 arg1Of upregulated,when
R4817 T5706 T5704 arg2Of injury,after
R4818 T5706 T5705 arg1Of injury,scratch
R4819 T5706 T5707 arg1Of injury,in
R4820 T5708 T5709 arg1Of WT,and
R4821 T5710 T5709 arg2Of KO,and
R4822 T5711 T5707 arg2Of astrocytes,in
R4823 T5711 T5708 arg1Of astrocytes,WT
R4824 T5711 T5710 arg1Of astrocytes,KO
R4825 T5713 T5712 arg2Of compared,when
R4826 T5713 T5714 arg1Of compared,with
R4827 T5717 T5714 arg2Of counterparts,with
R4828 T5717 T5715 arg1Of counterparts,their
R4829 T5717 T5716 arg1Of counterparts,sham
R4830 T5719 T5720 arg1Of it,was
R4831 T5719 T5722 arg1Of it,higher
R4832 T5720 T5718 arg1Of was,And
R4833 T5720 T5721 arg1Of was,also
R4834 T5720 T5732 arg1Of was,(
R4835 T5720 T5745 arg1Of was,(
R4836 T5720 T5751 arg1Of was,)
R4837 T5722 T5720 arg2Of higher,was
R4838 T5722 T5723 arg1Of higher,in
R4839 T5722 T5727 arg1Of higher,than
R4840 T5726 T5723 arg2Of TBI,in
R4841 T5726 T5724 arg1Of TBI,group
R4842 T5726 T5725 arg1Of TBI,KO
R4843 T5728 T5727 arg2Of in,than
R4844 T5731 T5728 arg2Of TBI,in
R4845 T5731 T5729 arg1Of TBI,group
R4846 T5731 T5730 arg1Of TBI,WT
R4847 T5735 T5736 arg1Of 0.156,versus
R4848 T5737 T5736 arg2Of 2.31,versus
R4849 T5737 T5738 arg1Of 2.31,±
R4850 T5739 T5732 arg2Of 0.087,(
R4851 T5739 T5733 arg1Of 0.087,3.67
R4852 T5739 T5734 arg1Of 0.087,±
R4853 T5739 T5735 arg1Of 0.087,0.156
R4854 T5739 T5740 arg1Of 0.087,","
R4855 T5741 T5740 arg2Of P,","
R4856 T5741 T5742 arg1Of P,<
R4857 T5741 T5743 arg1Of P,0.01
R4858 T5744 T5732 arg3Of ),(
R4859 T5746 T5745 arg2Of Figure,(
R4860 T5746 T5747 arg1Of Figure,4
R4861 T5746 T5748 arg1Of Figure,(
R4862 T5746 T5749 arg1Of Figure,a
R4863 T5750 T5745 arg3Of ),(
R4864 T5753 T5752 arg1Of tendency,Similar
R4865 T5753 T5754 arg1Of tendency,was
R4866 T5753 T5755 arg2Of tendency,observed
R4867 T5755 T5754 arg2Of observed,was
R4868 T5755 T5756 arg1Of observed,in
R4869 T5758 T5756 arg2Of level,in
R4870 T5758 T5757 arg1Of level,protein
R4871 T5758 T5759 arg1Of level,of
R4872 T5760 T5761 arg1Of IL-6,and
R4873 T5761 T5759 arg2Of and,of
R4874 T5762 T5761 arg2Of IL-1β,and
R4875 T5764 T5763 arg2Of IL-6,For
R4876 T5766 T5767 arg1Of it,was
R4877 T5767 T5763 arg1Of was,For
R4878 T5767 T5765 arg1Of was,","
R4879 T5767 T5775 arg1Of was,while
R4880 T5770 T5767 arg2Of 0.786,was
R4881 T5770 T5768 arg1Of 0.786,36.07
R4882 T5770 T5769 arg1Of 0.786,±
R4883 T5770 T5771 arg1Of 0.786,for
R4884 T5774 T5771 arg2Of TBI,for
R4885 T5774 T5772 arg1Of TBI,group
R4886 T5774 T5773 arg1Of TBI,KO
R4887 T5778 T5775 arg2Of 0.536,while
R4888 T5778 T5776 arg1Of 0.536,25.76
R4889 T5778 T5777 arg1Of 0.536,±
R4890 T5778 T5779 arg1Of 0.536,for
R4891 T5778 T5788 arg1Of 0.536,(
R4892 T5782 T5779 arg2Of TBI,for
R4893 T5782 T5780 arg1Of TBI,group
R4894 T5782 T5781 arg1Of TBI,WT
R4895 T5782 T5783 arg1Of TBI,(
R4896 T5784 T5783 arg2Of P,(
R4897 T5784 T5785 arg1Of P,<
R4898 T5784 T5786 arg1Of P,0.01
R4899 T5787 T5783 arg3Of ),(
R4900 T5789 T5788 arg2Of Figure,(
R4901 T5789 T5790 arg1Of Figure,4
R4902 T5789 T5791 arg1Of Figure,(
R4903 T5792 T5791 arg2Of b,(
R4904 T5793 T5791 arg3Of ),(
R4905 T5794 T5788 arg3Of ),(
R4906 T5796 T5795 arg2Of IL-1β,For
R4907 T5798 T5799 arg1Of it,was
R4908 T5799 T5795 arg1Of was,For
R4909 T5799 T5797 arg1Of was,","
R4910 T5799 T5807 arg1Of was,while
R4911 T5802 T5799 arg2Of 6.339,was
R4912 T5802 T5800 arg1Of 6.339,190.75
R4913 T5802 T5801 arg1Of 6.339,±
R4914 T5802 T5803 arg1Of 6.339,for
R4915 T5806 T5803 arg2Of TBI,for
R4916 T5806 T5804 arg1Of TBI,group
R4917 T5806 T5805 arg1Of TBI,KO
R4918 T5810 T5807 arg2Of 5.348,while
R4919 T5810 T5808 arg1Of 5.348,154.50
R4920 T5810 T5809 arg1Of 5.348,±
R4921 T5810 T5811 arg1Of 5.348,for
R4922 T5810 T5820 arg1Of 5.348,(
R4923 T5814 T5811 arg2Of TBI,for
R4924 T5814 T5812 arg1Of TBI,group
R4925 T5814 T5813 arg1Of TBI,WT
R4926 T5814 T5815 arg1Of TBI,(
R4927 T5816 T5815 arg2Of P,(
R4928 T5816 T5817 arg1Of P,<
R4929 T5816 T5818 arg1Of P,0.01
R4930 T5819 T5815 arg3Of ),(
R4931 T5821 T5820 arg2Of Figure,(
R4932 T5821 T5822 arg1Of Figure,4
R4933 T5821 T5823 arg1Of Figure,(
R4934 T5824 T5823 arg2Of c,(
R4935 T5825 T5823 arg3Of ),(
R4936 T5826 T5820 arg3Of ),(
R5381 T6504 T6505 arg1Of Expression,and
R5382 T6505 T6507 arg1Of and,of
R5383 T6505 T6509 arg1Of and,Were
R5384 T6505 T6511 arg2Of and,Enhanced
R5385 T6506 T6505 arg2Of Activity,and
R5386 T6508 T6507 arg2Of MMP9,of
R5387 T6511 T6509 arg2Of Enhanced,Were
R5388 T6511 T6510 arg1Of Enhanced,Greatly
R5389 T6511 T6512 arg1Of Enhanced,in
R5390 T6511 T6516 arg1Of Enhanced,after
R5391 T6515 T6512 arg2Of Astrocytes,in
R5392 T6515 T6513 arg1Of Astrocytes,Nrf2
R5393 T6515 T6514 arg1Of Astrocytes,Knockout
R5394 T6518 T6516 arg2Of Injury,after
R5395 T6518 T6517 arg1Of Injury,Scratch
R5396 T6519 T6520 arg1Of MMP9,is
R5397 T6523 T6520 arg2Of gelatinase,is
R5398 T6523 T6521 arg1Of gelatinase,an
R5399 T6523 T6522 arg1Of gelatinase,important
R5400 T6523 T6524 modOf gelatinase,to
R5401 T6525 T6526 arg1Of induce,or
R5402 T6526 T6524 arg1Of or,to
R5403 T6527 T6526 arg2Of aggravate,or
R5404 T6530 T6525 arg2Of process,induce
R5405 T6530 T6527 arg2Of process,aggravate
R5406 T6530 T6528 arg1Of process,the
R5407 T6530 T6529 arg1Of process,inflammation
R5408 T6534 T6531 arg2Of study,In
R5409 T6534 T6532 arg1Of study,the
R5410 T6534 T6533 arg1Of study,present
R5411 T6536 T6537 arg1Of we,elevated
R5412 T6537 T6531 arg1Of elevated,In
R5413 T6537 T6535 arg1Of elevated,","
R5414 T6539 T6538 arg1Of mRNA,the
R5415 T6539 T6540 arg1Of mRNA,","
R5416 T6540 T6544 arg1Of ",",and
R5417 T6542 T6540 arg2Of levels,","
R5418 T6542 T6541 arg1Of levels,protein
R5419 T6544 T6537 arg2Of and,elevated
R5420 T6544 T6543 arg1Of and,","
R5421 T6545 T6544 arg2Of activity,and
R5422 T6545 T6546 arg1Of activity,of
R5423 T6547 T6546 arg2Of MMP9,of
R5424 T6547 T6548 arg1Of MMP9,in
R5425 T6549 T6548 arg2Of astrocytes,in
R5426 T6549 T6550 arg1Of astrocytes,at
R5427 T6549 T6553 arg1Of astrocytes,after
R5428 T6552 T6550 arg2Of h,at
R5429 T6552 T6551 arg1Of h,24
R5430 T6555 T6553 arg2Of injury,after
R5431 T6555 T6554 arg1Of injury,scratch
R5432 T6555 T6556 arg1Of injury,by
R5433 T6557 T6558 arg1Of RT-PCR,","
R5434 T6558 T6562 arg1Of ",",and
R5435 T6560 T6558 arg2Of blot,","
R5436 T6560 T6559 arg1Of blot,western
R5437 T6562 T6556 arg2Of and,by
R5438 T6562 T6561 arg1Of and,","
R5439 T6564 T6562 arg2Of zymography,and
R5440 T6564 T6563 arg1Of zymography,gelatine
R5441 T6565 T6566 arg1Of Expression,of
R5442 T6565 T6568 arg1Of Expression,was
R5443 T6565 T6569 arg2Of Expression,elevated
R5444 T6567 T6566 arg2Of MMP9,of
R5445 T6569 T6568 arg2Of elevated,was
R5446 T6569 T6570 arg1Of elevated,after
R5447 T6572 T6570 arg2Of injury,after
R5448 T6572 T6571 arg1Of injury,scratch
R5449 T6572 T6573 arg1Of injury,in
R5450 T6572 T6578 arg1Of injury,as
R5451 T6574 T6575 arg1Of WT,and
R5452 T6576 T6575 arg2Of KO,and
R5453 T6577 T6573 arg2Of astrocytes,in
R5454 T6577 T6574 arg1Of astrocytes,WT
R5455 T6577 T6576 arg1Of astrocytes,KO
R5456 T6579 T6578 arg2Of compared,as
R5457 T6579 T6580 arg1Of compared,with
R5458 T6583 T6580 arg2Of counterparts,with
R5459 T6583 T6581 arg1Of counterparts,their
R5460 T6583 T6582 arg1Of counterparts,sham
R5461 T6586 T6584 arg1Of level,The
R5462 T6586 T6585 arg1Of level,mRNA
R5463 T6586 T6587 arg1Of level,of
R5464 T6586 T6589 arg1Of level,was
R5465 T6586 T6590 arg1Of level,higher
R5466 T6588 T6587 arg2Of MMP9,of
R5467 T6589 T6600 arg1Of was,(
R5468 T6589 T6613 arg1Of was,(
R5469 T6590 T6589 arg2Of higher,was
R5470 T6590 T6591 arg1Of higher,in
R5471 T6590 T6595 arg1Of higher,than
R5472 T6594 T6591 arg2Of TBI,in
R5473 T6594 T6592 arg1Of TBI,group
R5474 T6594 T6593 arg1Of TBI,KO
R5475 T6596 T6595 arg2Of in,than
R5476 T6599 T6596 arg2Of TBI,in
R5477 T6599 T6597 arg1Of TBI,group
R5478 T6599 T6598 arg1Of TBI,WT
R5479 T6603 T6604 arg1Of 0.090,versus
R5480 T6605 T6604 arg2Of 1.01,versus
R5481 T6605 T6606 arg1Of 1.01,±
R5482 T6607 T6600 arg2Of 0.068,(
R5483 T6607 T6601 arg1Of 0.068,1.36
R5484 T6607 T6602 arg1Of 0.068,±
R5485 T6607 T6603 arg1Of 0.068,0.090
R5486 T6607 T6608 arg1Of 0.068,","
R5487 T6609 T6608 arg2Of P,","
R5488 T6609 T6610 arg1Of P,<
R5489 T6609 T6611 arg1Of P,0.01
R5490 T6612 T6600 arg3Of ),(
R5491 T6615 T6614 arg1Of 3,Figures
R5492 T6615 T6616 arg1Of 3,(
R5493 T6615 T6617 arg1Of 3,a
R5494 T6615 T6618 arg1Of 3,)
R5495 T6615 T6619 arg1Of 3,and
R5496 T6619 T6613 arg2Of and,(
R5497 T6620 T6619 arg2Of 3,and
R5498 T6620 T6621 arg1Of 3,(
R5499 T6622 T6621 arg2Of e,(
R5500 T6623 T6621 arg3Of ),(
R5501 T6624 T6613 arg3Of ),(
R5502 T6626 T6625 arg1Of difference,Significant
R5503 T6626 T6627 arg1Of difference,was
R5504 T6626 T6629 arg2Of difference,discovered
R5505 T6629 T6627 arg2Of discovered,was
R5506 T6629 T6628 arg1Of discovered,also
R5507 T6629 T6630 arg1Of discovered,in
R5508 T6629 T6637 arg1Of discovered,","
R5509 T6629 T6638 arg1Of discovered,as
R5510 T6633 T6630 arg2Of level,in
R5511 T6633 T6631 arg1Of level,MMP9
R5512 T6633 T6632 arg1Of level,protein
R5513 T6636 T6629 arg1Of blot,discovered
R5514 T6636 T6634 arg2Of blot,by
R5515 T6636 T6635 arg1Of blot,western
R5516 T6641 T6638 arg2Of 0.076,as
R5517 T6641 T6639 arg1Of 0.076,1.44
R5518 T6641 T6640 arg1Of 0.076,±
R5519 T6641 T6642 arg1Of 0.076,for
R5520 T6645 T6643 arg1Of TBI,group
R5521 T6645 T6644 arg1Of TBI,KO
R5522 T6645 T6646 arg1Of TBI,and
R5523 T6646 T6642 arg2Of and,for
R5524 T6647 T6648 arg1Of 1.13,±
R5525 T6649 T6646 arg2Of 0.048,and
R5526 T6649 T6647 arg1Of 0.048,1.13
R5527 T6649 T6650 arg1Of 0.048,for
R5528 T6649 T6659 arg1Of 0.048,(
R5529 T6653 T6650 arg2Of TBI,for
R5530 T6653 T6651 arg1Of TBI,group
R5531 T6653 T6652 arg1Of TBI,WT
R5532 T6653 T6654 arg1Of TBI,(
R5533 T6655 T6654 arg2Of P,(
R5534 T6655 T6656 arg1Of P,<
R5535 T6655 T6657 arg1Of P,0.01
R5536 T6658 T6654 arg3Of ),(
R5537 T6660 T6659 arg2Of Figure,(
R5538 T6660 T6661 arg1Of Figure,5
R5539 T6662 T6659 arg3Of ),(
R5540 T6664 T6663 arg1Of zymography,Gelatine
R5541 T6664 T6665 arg1Of zymography,revealed
R5542 T6668 T6665 arg2Of activity,revealed
R5543 T6668 T6666 arg1Of activity,higher
R5544 T6668 T6667 arg1Of activity,MMP9
R5545 T6668 T6669 arg1Of activity,in
R5546 T6668 T6673 arg1Of activity,than
R5547 T6668 T6679 arg1Of activity,too
R5548 T6668 T6693 arg1Of activity,(
R5549 T6672 T6669 arg2Of TBI,in
R5550 T6672 T6670 arg1Of TBI,group
R5551 T6672 T6671 arg1Of TBI,KO
R5552 T6674 T6673 arg2Of that,than
R5553 T6674 T6675 arg1Of that,in
R5554 T6678 T6675 arg2Of TBI,in
R5555 T6678 T6676 arg1Of TBI,group
R5556 T6678 T6677 arg1Of TBI,WT
R5557 T6679 T6680 arg1Of too,(
R5558 T6683 T6684 arg1Of 6.343,versus
R5559 T6685 T6684 arg2Of 76.32,versus
R5560 T6685 T6686 arg1Of 76.32,±
R5561 T6687 T6680 arg2Of 3.388,(
R5562 T6687 T6681 arg1Of 3.388,101.76
R5563 T6687 T6682 arg1Of 3.388,±
R5564 T6687 T6683 arg1Of 3.388,6.343
R5565 T6687 T6688 arg1Of 3.388,","
R5566 T6689 T6688 arg2Of P,","
R5567 T6689 T6690 arg1Of P,<
R5568 T6689 T6691 arg1Of P,0.01
R5569 T6692 T6680 arg3Of ),(
R5570 T6694 T6693 arg2Of Figure,(
R5571 T6694 T6695 arg1Of Figure,6
R5572 T6696 T6693 arg3Of ),(
R5909 T7370 T7368 arg1Of study,The
R5910 T7370 T7369 arg1Of study,present
R5911 T7370 T7371 arg1Of study,demonstrated
R5912 T7374 T7373 arg1Of injury,scratch
R5913 T7374 T7375 arg1Of injury,induced
R5914 T7375 T7371 arg2Of induced,demonstrated
R5915 T7375 T7372 arg1Of induced,that
R5916 T7377 T7376 arg1Of upregulation,the
R5917 T7377 T7378 arg1Of upregulation,of
R5918 T7377 T7383 arg1Of upregulation,and
R5919 T7382 T7378 arg2Of activity,of
R5920 T7382 T7379 arg1Of activity,NF-κB
R5921 T7382 T7380 arg1Of activity,DNA
R5922 T7382 T7381 arg1Of activity,binding
R5923 T7383 T7375 arg2Of and,induced
R5924 T7384 T7383 arg2Of overexpression,and
R5925 T7384 T7385 arg1Of overexpression,of
R5926 T7386 T7387 arg1Of TNF-α,","
R5927 T7387 T7389 arg1Of ",",","
R5928 T7388 T7387 arg2Of IL-1β,","
R5929 T7389 T7392 arg1Of ",",and
R5930 T7390 T7389 arg2Of IL-6,","
R5931 T7392 T7385 arg2Of and,of
R5932 T7392 T7391 arg1Of and,","
R5933 T7392 T7394 arg1Of and,in
R5934 T7393 T7392 arg2Of MMP9,and
R5935 T7396 T7394 arg2Of astrocytes,in
R5936 T7396 T7395 arg2Of astrocytes,cultured
R5937 T7398 T7399 arg1Of we,revealed
R5938 T7399 T7397 arg1Of revealed,Also
R5939 T7402 T7401 arg1Of injury,scratch
R5940 T7402 T7403 arg1Of injury,induced
R5941 T7403 T7399 arg2Of induced,revealed
R5942 T7403 T7400 arg1Of induced,that
R5943 T7405 T7404 arg1Of activity,higher
R5944 T7405 T7406 arg1Of activity,of
R5945 T7405 T7408 arg1Of activity,and
R5946 T7407 T7406 arg2Of NF-κB,of
R5947 T7408 T7403 arg2Of and,induced
R5948 T7410 T7408 arg2Of expression,and
R5949 T7410 T7409 arg2Of expression,enhanced
R5950 T7410 T7411 arg1Of expression,of
R5951 T7410 T7414 arg1Of expression,in
R5952 T7413 T7411 arg2Of cytokines,of
R5953 T7413 T7412 arg1Of cytokines,proinflammatory
R5954 T7418 T7414 arg2Of astrocytes,in
R5955 T7418 T7415 arg1Of astrocytes,Nrf2
R5956 T7418 T7416 arg1Of astrocytes,knockout
R5957 T7418 T7417 arg2Of astrocytes,cultured
R5958 T7418 T7419 arg1Of astrocytes,than
R5959 T7420 T7419 arg2Of those,than
R5960 T7420 T7421 arg1Of those,in
R5961 T7423 T7421 arg2Of astrocytes,in
R5962 T7423 T7422 arg1Of astrocytes,wildtype
R5963 T7423 T7424 arg1Of astrocytes,for
R5964 T7427 T7424 arg2Of time,for
R5965 T7427 T7425 arg1Of time,the
R5966 T7427 T7426 arg1Of time,first
R5967 T7428 T7429 arg1Of It,has
R5968 T7428 T7430 arg1Of It,been
R5969 T7428 T7431 arg2Of It,demonstrated
R5970 T7431 T7429 arg2Of demonstrated,has
R5971 T7431 T7430 arg2Of demonstrated,been
R5972 T7433 T7434 arg1Of NF-κB,is
R5973 T7433 T7435 arg2Of NF-κB,activated
R5974 T7435 T7431 arg3Of activated,demonstrated
R5975 T7435 T7432 arg1Of activated,that
R5976 T7435 T7434 arg2Of activated,is
R5977 T7435 T7436 arg1Of activated,in
R5978 T7435 T7441 arg1Of activated,after
R5979 T7435 T7444 arg1Of activated,[
R5980 T7437 T7438 arg1Of brain,and
R5981 T7438 T7436 arg2Of and,in
R5982 T7440 T7438 arg2Of cord,and
R5983 T7440 T7439 arg1Of cord,spinal
R5984 T7443 T7441 arg2Of injury,after
R5985 T7443 T7442 arg1Of injury,traumatic
R5986 T7445 T7444 arg2Of 2,[
R5987 T7445 T7446 arg1Of 2,","
R5988 T7447 T7446 arg2Of 9,","
R5989 T7448 T7444 arg3Of ],[
R5990 T7452 T7449 arg2Of factor,As
R5991 T7452 T7450 arg1Of factor,a
R5992 T7452 T7451 arg1Of factor,transcript
R5993 T7454 T7455 arg1Of NF-κB,binds
R5994 T7454 T7463 arg1Of NF-κB,induces
R5995 T7455 T7456 arg1Of binds,with
R5996 T7455 T7458 arg1Of binds,once
R5997 T7455 T7462 arg1Of binds,and
R5998 T7457 T7456 arg2Of DNA,with
R5999 T7459 T7460 arg1Of it,was
R6000 T7459 T7461 arg2Of it,activated
R6001 T7461 T7458 arg2Of activated,once
R6002 T7461 T7460 arg2Of activated,was
R6003 T7462 T7449 arg1Of and,As
R6004 T7462 T7453 arg1Of and,","
R6005 T7463 T7462 arg2Of induces,and
R6006 T7464 T7463 arg2Of transcription,induces
R6007 T7464 T7465 arg1Of transcription,of
R6008 T7464 T7470 arg1Of transcription,of
R6009 T7466 T7467 arg1Of MMP9,and
R6010 T7467 T7465 arg2Of and,of
R6011 T7469 T7467 arg2Of battery,and
R6012 T7469 T7468 arg1Of battery,a
R6013 T7472 T7470 arg2Of cytokines,of
R6014 T7472 T7471 arg1Of cytokines,proinflammatory
R6015 T7472 T7473 arg1Of cytokines,","
R6016 T7472 T7474 arg1Of cytokines,including
R6017 T7475 T7476 arg1Of TNF-α,","
R6018 T7476 T7479 arg1Of ",",and
R6019 T7477 T7476 arg2Of IL-1β,","
R6020 T7479 T7474 arg2Of and,including
R6021 T7479 T7478 arg1Of and,","
R6022 T7479 T7481 arg1Of and,","
R6023 T7479 T7482 arg1Of and,in
R6024 T7479 T7485 arg1Of and,after
R6025 T7480 T7479 arg2Of IL-6,and
R6026 T7484 T7482 arg2Of tissues,in
R6027 T7484 T7483 arg1Of tissues,brain
R6028 T7486 T7485 arg2Of TBI,after
R6029 T7486 T7487 arg1Of TBI,[
R6030 T7488 T7489 arg1Of 3,","
R6031 T7489 T7487 arg2Of ",",[
R6032 T7489 T7491 arg1Of ",",","
R6033 T7490 T7489 arg2Of 10,","
R6034 T7492 T7491 arg2Of 11,","
R6035 T7493 T7487 arg3Of ],[
R6036 T7495 T7494 arg1Of results,Our
R6037 T7495 T7497 arg1Of results,revealed
R6038 T7497 T7496 arg1Of revealed,also
R6039 T7500 T7499 arg1Of injury,scratch
R6040 T7500 T7501 arg1Of injury,induced
R6041 T7501 T7497 arg2Of induced,revealed
R6042 T7501 T7498 arg1Of induced,that
R6043 T7502 T7501 arg2Of elevation,induced
R6044 T7502 T7503 arg1Of elevation,of
R6045 T7507 T7504 arg1Of activity,NF-κB
R6046 T7507 T7505 arg1Of activity,DNA
R6047 T7507 T7506 arg1Of activity,binding
R6048 T7507 T7508 arg1Of activity,","
R6049 T7508 T7513 arg1Of ",",and
R6050 T7509 T7508 arg2Of overexpression,","
R6051 T7509 T7510 arg1Of overexpression,of
R6052 T7511 T7510 arg2Of MMP9,of
R6053 T7513 T7503 arg2Of and,of
R6054 T7513 T7512 arg1Of and,","
R6055 T7515 T7513 arg2Of mediators,and
R6056 T7515 T7514 arg1Of mediators,proinflammatory
R6057 T7515 T7516 arg2Of mediators,mentioned
R6058 T7516 T7517 arg1Of mentioned,above
R6059 T7516 T7518 arg1Of mentioned,in
R6060 T7520 T7518 arg2Of astrocytes,in
R6061 T7520 T7519 arg2Of astrocytes,cultured
R6062 T7521 T7522 arg1Of It,has
R6063 T7521 T7523 arg1Of It,been
R6064 T7521 T7524 arg2Of It,demonstrated
R6065 T7524 T7522 arg2Of demonstrated,has
R6066 T7524 T7523 arg2Of demonstrated,been
R6067 T7526 T7527 arg1Of there,is
R6068 T7527 T7524 arg3Of is,demonstrated
R6069 T7527 T7525 arg1Of is,that
R6070 T7530 T7527 arg2Of loop,is
R6071 T7530 T7528 arg1Of loop,an
R6072 T7530 T7529 arg1Of loop,autoregulatory
R6073 T7530 T7531 arg1Of loop,among
R6074 T7534 T7531 arg2Of mediators,among
R6075 T7534 T7532 arg1Of mediators,these
R6076 T7534 T7533 arg1Of mediators,proinflammatory
R6077 T7536 T7535 arg2Of example,For
R6078 T7538 T7539 arg1Of TNF-α,and
R6079 T7539 T7541 arg1Of and,are
R6080 T7540 T7539 arg2Of IL-1β,and
R6081 T7541 T7554 arg1Of are,and
R6082 T7543 T7541 arg2Of stimulators,are
R6083 T7543 T7542 arg1Of stimulators,potent
R6084 T7543 T7544 arg1Of stimulators,for
R6085 T7543 T7546 arg1Of stimulators,in
R6086 T7545 T7544 arg2Of MMP9,for
R6087 T7547 T7546 arg2Of astrocytes,in
R6088 T7547 T7548 arg1Of astrocytes,[
R6089 T7549 T7550 arg1Of 12,","
R6090 T7550 T7548 arg2Of ",",[
R6091 T7551 T7550 arg2Of 13,","
R6092 T7552 T7548 arg3Of ],[
R6093 T7554 T7535 arg1Of and,For
R6094 T7554 T7537 arg1Of and,","
R6095 T7554 T7553 arg1Of and,","
R6096 T7555 T7556 arg1Of IL-6,induces
R6097 T7556 T7560 arg1Of induces,in
R6098 T7556 T7569 arg1Of induces,and
R6099 T7557 T7556 arg2Of overexpression,induces
R6100 T7557 T7558 arg1Of overexpression,of
R6101 T7559 T7558 arg2Of MMP9,of
R6102 T7564 T7560 arg2Of cells,in
R6103 T7564 T7561 arg1Of cells,human
R6104 T7564 T7562 arg1Of cells,colon
R6105 T7564 T7563 arg1Of cells,carcinoma
R6106 T7564 T7565 arg1Of cells,[
R6107 T7566 T7565 arg2Of 14,[
R6108 T7567 T7565 arg3Of ],[
R6109 T7569 T7554 arg2Of and,and
R6110 T7569 T7568 arg1Of and,","
R6111 T7570 T7571 arg1Of TNF-α,and
R6112 T7571 T7574 arg1Of and,can
R6113 T7571 T7575 arg1Of and,induce
R6114 T7572 T7571 arg2Of IL-1β,and
R6115 T7575 T7569 arg2Of induce,and
R6116 T7575 T7573 arg1Of induce,also
R6117 T7575 T7574 arg2Of induce,can
R6118 T7575 T7579 arg1Of induce,[
R6119 T7576 T7575 arg2Of activation,induce
R6120 T7576 T7577 arg1Of activation,of
R6121 T7578 T7577 arg2Of NF-κB,of
R6122 T7580 T7579 arg2Of 15,[
R6123 T7580 T7581 arg1Of 15,","
R6124 T7582 T7581 arg2Of 16,","
R6125 T7583 T7579 arg3Of ],[
R6126 T7586 T7584 arg1Of loop,This
R6127 T7586 T7585 arg1Of loop,autoregulatory
R6128 T7586 T7588 arg1Of loop,aggravates
R6129 T7586 T7595 arg1Of loop,induces
R6130 T7588 T7594 arg1Of aggravates,and
R6131 T7591 T7588 arg2Of effect,aggravates
R6132 T7591 T7589 arg1Of effect,the
R6133 T7591 T7590 arg1Of effect,damaging
R6134 T7591 T7592 arg1Of effect,of
R6135 T7593 T7592 arg2Of inflammation,of
R6136 T7594 T7587 arg1Of and,extremely
R6137 T7595 T7594 arg2Of induces,and
R6138 T7597 T7595 arg2Of injury,induces
R6139 T7597 T7596 arg1Of injury,secondary
R6140 T7597 T7598 arg1Of injury,to
R6141 T7599 T7598 arg2Of brain,to
R6142 T7600 T7601 arg1Of It,is
R6143 T7604 T7601 arg2Of choice,is
R6144 T7604 T7602 arg1Of choice,a
R6145 T7604 T7603 arg1Of choice,good
R6146 T7604 T7605 arg1Of choice,for
R6147 T7606 T7605 arg2Of targeting,for
R6148 T7606 T7607 arg1Of targeting,on
R6149 T7606 T7611 modOf targeting,to
R6150 T7610 T7606 arg2Of factor,targeting
R6151 T7610 T7608 arg1Of factor,an
R6152 T7610 T7609 arg1Of factor,upstream
R6153 T7612 T7611 arg1Of prevent,to
R6154 T7612 T7616 arg1Of prevent,after
R6155 T7615 T7612 arg2Of loop,prevent
R6156 T7615 T7613 arg1Of loop,such
R6157 T7615 T7614 arg1Of loop,autoregulatory
R6158 T7617 T7616 arg2Of TBI,after
R6159 T7619 T7618 arg1Of pathway,Nrf2-ARE
R6160 T7619 T7620 arg1Of pathway,has
R6161 T7619 T7621 arg1Of pathway,been
R6162 T7619 T7622 arg2Of pathway,proved
R6163 T7619 T7624 arg1Of pathway,be
R6164 T7622 T7620 arg2Of proved,has
R6165 T7622 T7621 arg2Of proved,been
R6166 T7624 T7622 arg3Of be,proved
R6167 T7624 T7623 arg1Of be,to
R6168 T7627 T7624 arg2Of regulator,be
R6169 T7627 T7625 arg1Of regulator,the
R6170 T7627 T7626 arg1Of regulator,key
R6171 T7627 T7628 arg1Of regulator,in
R6172 T7629 T7628 arg2Of reducing,in
R6173 T7631 T7630 arg1Of stress,oxidative
R6174 T7631 T7632 arg1Of stress,","
R6175 T7632 T7636 arg1Of ",",and
R6176 T7634 T7632 arg2Of damage,","
R6177 T7634 T7633 arg1Of damage,inflammatory
R6178 T7636 T7629 arg2Of and,reducing
R6179 T7636 T7635 arg1Of and,","
R6180 T7637 T7636 arg2Of accumulation,and
R6181 T7637 T7638 arg1Of accumulation,of
R6182 T7640 T7638 arg2Of metabolites,of
R6183 T7640 T7639 arg1Of metabolites,toxic
R6184 T7640 T7641 arg1Of metabolites,","
R6185 T7640 T7642 arg1Of metabolites,which
R6186 T7640 T7643 arg1Of metabolites,are
R6187 T7640 T7645 arg2Of metabolites,involved
R6188 T7645 T7643 arg2Of involved,are
R6189 T7645 T7644 arg1Of involved,all
R6190 T7645 T7646 arg1Of involved,in
R6191 T7647 T7646 arg2Of TBI,in
R6192 T7650 T7648 arg1Of study,Our
R6193 T7650 T7649 arg1Of study,previous
R6194 T7650 T7651 arg1Of study,has
R6195 T7650 T7652 arg1Of study,proved
R6196 T7652 T7651 arg2Of proved,has
R6197 T7652 T7660 arg1Of proved,after
R6198 T7654 T7652 arg2Of augmentation,proved
R6199 T7654 T7653 arg1Of augmentation,the
R6200 T7654 T7655 arg1Of augmentation,of
R6201 T7654 T7657 arg1Of augmentation,in
R6202 T7656 T7655 arg2Of Nrf2,of
R6203 T7659 T7657 arg2Of tissue,in
R6204 T7659 T7658 arg1Of tissue,brain
R6205 T7661 T7660 arg2Of TBI,after
R6206 T7661 T7662 arg1Of TBI,[
R6207 T7663 T7662 arg2Of 17,[
R6208 T7664 T7662 arg3Of ],[
R6209 T7666 T7665 arg2Of level,Enhanced
R6210 T7666 T7667 arg1Of level,of
R6211 T7666 T7669 arg1Of level,activates
R6212 T7668 T7667 arg2Of Nrf2,of
R6213 T7670 T7669 arg2Of transcription,activates
R6214 T7670 T7671 arg1Of transcription,of
R6215 T7670 T7677 arg1Of transcription,","
R6216 T7670 T7679 arg1Of transcription,as
R6217 T7673 T7671 arg2Of group,of
R6218 T7673 T7672 arg1Of group,a
R6219 T7673 T7674 arg1Of group,of
R6220 T7676 T7674 arg2Of genes,of
R6221 T7676 T7675 arg1Of genes,antioxidant
R6222 T7679 T7678 arg1Of as,such
R6223 T7681 T7680 arg1Of oxygenase-1,heme
R6224 T7681 T7682 arg1Of oxygenase-1,(
R6225 T7681 T7685 arg1Of oxygenase-1,and
R6226 T7683 T7682 arg2Of HO-1,(
R6227 T7684 T7682 arg3Of ),(
R6228 T7685 T7679 arg2Of and,as
R6229 T7685 T7691 arg1Of and,:
R6230 T7688 T7687 arg2Of P,(
R6231 T7689 T7687 arg3Of ),(
R6232 T7690 T7685 arg2Of H,and
R6233 T7690 T7686 arg1Of H,NAD
R6234 T7690 T7687 arg1Of H,(
R6235 T7693 T7691 arg2Of oxidoreductase-1,:
R6236 T7693 T7692 arg1Of oxidoreductase-1,quinone
R6237 T7693 T7694 arg1Of oxidoreductase-1,(
R6238 T7693 T7697 arg1Of oxidoreductase-1,","
R6239 T7693 T7698 arg1Of oxidoreductase-1,which
R6240 T7693 T7699 arg1Of oxidoreductase-1,would
R6241 T7693 T7701 arg1Of oxidoreductase-1,reduce
R6242 T7695 T7694 arg2Of NQO1,(
R6243 T7696 T7694 arg3Of ),(
R6244 T7701 T7699 arg2Of reduce,would
R6245 T7701 T7700 arg1Of reduce,subsequently
R6246 T7703 T7701 arg2Of damage,reduce
R6247 T7703 T7702 arg1Of damage,the
R6248 T7703 T7704 arg1Of damage,in
R6249 T7705 T7704 arg2Of brain,in
R6250 T7705 T7706 arg1Of brain,[
R6251 T7707 T7706 arg2Of 18,[
R6252 T7708 T7706 arg3Of ],[
R6253 T7710 T7709 arg1Of administration,Postinjury
R6254 T7710 T7711 arg1Of administration,of
R6255 T7710 T7720 arg1Of administration,improves
R6256 T7710 T7726 arg1Of administration,decreases
R6257 T7712 T7711 arg2Of SFN,of
R6258 T7712 T7713 arg1Of SFN,","
R6259 T7715 T7713 arg2Of inducer,","
R6260 T7715 T7714 arg1Of inducer,an
R6261 T7715 T7716 arg1Of inducer,of
R6262 T7717 T7716 arg2Of Nrf2,of
R6263 T7720 T7725 arg1Of improves,and
R6264 T7722 T7720 arg2Of memory,improves
R6265 T7722 T7721 arg1Of memory,spatial
R6266 T7722 T7723 arg1Of memory,of
R6267 T7724 T7723 arg2Of rat,of
R6268 T7725 T7718 arg1Of and,","
R6269 T7725 T7719 arg1Of and,significantly
R6270 T7726 T7725 arg2Of decreases,and
R6271 T7728 T7726 arg2Of immunoreactivity,decreases
R6272 T7728 T7727 arg1Of immunoreactivity,the
R6273 T7728 T7729 arg1Of immunoreactivity,for
R6274 T7728 T7740 arg1Of immunoreactivity,","
R6275 T7728 T7741 arg1Of immunoreactivity,in
R6276 T7730 T7729 arg2Of 4-Hydroxynonenal,for
R6277 T7730 T7731 arg1Of 4-Hydroxynonenal,(
R6278 T7730 T7734 arg1Of 4-Hydroxynonenal,","
R6279 T7732 T7731 arg2Of 4-HNE,(
R6280 T7733 T7731 arg3Of ),(
R6281 T7736 T7734 arg2Of marker,","
R6282 T7736 T7735 arg1Of marker,a
R6283 T7736 T7737 arg1Of marker,of
R6284 T7739 T7737 arg2Of peroxidation,of
R6285 T7739 T7738 arg1Of peroxidation,lipid
R6286 T7743 T7742 arg1Of cortex,the
R6287 T7743 T7744 arg1Of cortex,and
R6288 T7744 T7741 arg2Of and,in
R6289 T7747 T7744 arg2Of subfield,and
R6290 T7747 T7745 arg1Of subfield,the
R6291 T7747 T7746 arg1Of subfield,CA3
R6292 T7747 T7748 arg1Of subfield,of
R6293 T7747 T7750 arg1Of subfield,after
R6294 T7749 T7748 arg2Of hippocampus,of
R6295 T7751 T7750 arg2Of TBI,after
R6296 T7751 T7752 arg1Of TBI,[
R6297 T7753 T7752 arg2Of 19,[
R6298 T7754 T7752 arg3Of ],[
R6299 T7758 T7755 arg2Of hand,On
R6300 T7758 T7756 arg1Of hand,the
R6301 T7758 T7757 arg1Of hand,other
R6302 T7761 T7760 arg1Of mice,Nrf2-deficient
R6303 T7761 T7762 arg1Of mice,appear
R6304 T7761 T7764 arg1Of mice,susceptible
R6305 T7762 T7755 arg1Of appear,On
R6306 T7762 T7759 arg1Of appear,","
R6307 T7764 T7762 arg2Of susceptible,appear
R6308 T7764 T7763 arg1Of susceptible,more
R6309 T7764 T7765 arg1Of susceptible,to
R6310 T7766 T7765 arg2Of TBI,to
R6311 T7767 T7768 arg1Of Depletion,of
R6312 T7767 T7770 arg1Of Depletion,induces
R6313 T7769 T7768 arg2Of Nrf2,of
R6314 T7770 T7776 arg1Of induces,in
R6315 T7772 T7770 arg2Of expression,induces
R6316 T7772 T7771 arg1Of expression,higher
R6317 T7772 T7773 arg1Of expression,of
R6318 T7775 T7773 arg2Of mediators,of
R6319 T7775 T7774 arg1Of mediators,proinflammatory
R6320 T7777 T7776 arg2Of brain,in
R6321 T7777 T7778 arg1Of brain,after
R6322 T7779 T7778 arg2Of TBI,after
R6323 T7779 T7780 arg1Of TBI,[
R6324 T7781 T7780 arg2Of 20,[
R6325 T7782 T7780 arg3Of ],[
R6326 T7786 T7785 arg1Of results,our
R6327 T7786 T7787 arg1Of results,revealed
R6328 T7787 T7783 arg1Of revealed,Here
R6329 T7787 T7784 arg1Of revealed,","
R6330 T7789 T7790 arg1Of overexpression,of
R6331 T7789 T7802 arg1Of overexpression,was
R6332 T7789 T7804 arg2Of overexpression,aggravated
R6333 T7791 T7792 arg1Of TNF-α,","
R6334 T7792 T7794 arg1Of ",",","
R6335 T7793 T7792 arg2Of IL-1β,","
R6336 T7794 T7797 arg1Of ",",and
R6337 T7795 T7794 arg2Of IL-6,","
R6338 T7797 T7790 arg2Of and,of
R6339 T7797 T7796 arg1Of and,","
R6340 T7797 T7799 arg1Of and,after
R6341 T7798 T7797 arg2Of MMP9,and
R6342 T7801 T7799 arg2Of injury,after
R6343 T7801 T7800 arg1Of injury,scratch
R6344 T7804 T7802 arg2Of aggravated,was
R6345 T7804 T7803 arg1Of aggravated,more
R6346 T7804 T7805 arg1Of aggravated,in
R6347 T7804 T7810 arg1Of aggravated,than
R6348 T7804 T7814 arg1Of aggravated,for
R6349 T7804 T7819 arg1Of aggravated,and
R6350 T7809 T7805 arg2Of astrocytes,in
R6351 T7809 T7806 arg2Of astrocytes,cultured
R6352 T7809 T7807 arg1Of astrocytes,Nrf2
R6353 T7809 T7808 arg1Of astrocytes,knockout
R6354 T7811 T7810 arg2Of in,than
R6355 T7813 T7811 arg2Of astrocyetes,in
R6356 T7813 T7812 arg1Of astrocyetes,wildtype
R6357 T7817 T7814 arg2Of time,for
R6358 T7817 T7815 arg1Of time,the
R6359 T7817 T7816 arg1Of time,first
R6360 T7819 T7787 arg2Of and,revealed
R6361 T7819 T7788 arg1Of and,that
R6362 T7819 T7818 arg1Of and,","
R6363 T7820 T7821 arg1Of overexpression,of
R6364 T7820 T7825 arg1Of overexpression,led
R6365 T7824 T7821 arg2Of mediators,of
R6366 T7824 T7822 arg1Of mediators,these
R6367 T7824 T7823 arg1Of mediators,proinflammatory
R6368 T7825 T7819 arg2Of led,and
R6369 T7825 T7826 arg1Of led,to
R6370 T7829 T7826 arg2Of deaths,to
R6371 T7829 T7827 arg1Of deaths,more
R6372 T7829 T7828 arg1Of deaths,astrocytes
R6373 T7830 T7831 arg2Of Data,obtained
R6374 T7830 T7835 arg1Of Data,suggest
R6375 T7831 T7832 arg1Of obtained,from
R6376 T7834 T7832 arg2Of studies,from
R6377 T7834 T7833 arg1Of studies,animal
R6378 T7837 T7835 arg2Of possibility,suggest
R6379 T7837 T7836 arg1Of possibility,the
R6380 T7840 T7839 arg1Of effect,antioxidant
R6381 T7840 T7841 arg1Of effect,of
R6382 T7840 T7843 arg1Of effect,may
R6383 T7840 T7844 arg1Of effect,be
R6384 T7840 T7845 arg2Of effect,achieved
R6385 T7842 T7841 arg2Of Nrf2,of
R6386 T7845 T7837 arg2Of achieved,possibility
R6387 T7845 T7838 arg1Of achieved,that
R6388 T7845 T7843 arg2Of achieved,may
R6389 T7845 T7844 arg2Of achieved,be
R6390 T7847 T7845 arg1Of suppression,achieved
R6391 T7847 T7846 arg2Of suppression,by
R6392 T7847 T7848 arg1Of suppression,of
R6393 T7850 T7848 arg2Of pathways,of
R6394 T7850 T7849 arg1Of pathways,proinflammatory
R6395 T7850 T7851 arg1Of pathways,which
R6396 T7850 T7852 arg1Of pathways,are
R6397 T7850 T7853 arg2Of pathways,mediated
R6398 T7853 T7852 arg2Of mediated,are
R6399 T7856 T7853 arg1Of signaling,mediated
R6400 T7856 T7854 arg2Of signaling,by
R6401 T7856 T7855 arg1Of signaling,NF-κB
R6402 T7857 T7858 arg1Of Administration,of
R6403 T7857 T7860 arg1Of Administration,is
R6404 T7857 T7861 arg2Of Administration,found
R6405 T7857 T7863 arg1Of Administration,be
R6406 T7857 T7864 arg1Of Administration,able
R6407 T7857 T7866 arg1Of Administration,inhibit
R6408 T7857 T7877 arg1Of Administration,alleviating
R6409 T7859 T7858 arg2Of SFN,of
R6410 T7861 T7860 arg2Of found,is
R6411 T7863 T7861 arg3Of be,found
R6412 T7863 T7862 arg1Of be,to
R6413 T7864 T7863 arg2Of able,be
R6414 T7866 T7864 arg2Of inhibit,able
R6415 T7866 T7865 arg1Of inhibit,to
R6416 T7866 T7875 arg1Of inhibit,","
R6417 T7866 T7877 modOf inhibit,alleviating
R6418 T7868 T7867 arg1Of phosphorylation,IKK/IκB
R6419 T7868 T7869 arg1Of phosphorylation,and
R6420 T7869 T7866 arg2Of and,inhibit
R6421 T7874 T7869 arg2Of translocation,and
R6422 T7874 T7870 arg1Of translocation,p65
R6423 T7874 T7871 arg1Of translocation,NF-κB
R6424 T7874 T7872 arg1Of translocation,subunit
R6425 T7874 T7873 arg1Of translocation,nuclear
R6426 T7877 T7876 arg1Of alleviating,consequently
R6427 T7879 T7877 arg2Of signaling,alleviating
R6428 T7879 T7878 arg1Of signaling,NF-κB
R6429 T7879 T7880 arg1Of signaling,[
R6430 T7881 T7880 arg2Of 21,[
R6431 T7882 T7880 arg3Of ],[
R6432 T7885 T7884 arg1Of activation,NF-κB
R6433 T7885 T7886 arg2Of activation,induced
R6434 T7885 T7892 arg1Of activation,could
R6435 T7885 T7893 arg1Of activation,be
R6436 T7885 T7894 arg2Of activation,attenuated
R6437 T7888 T7886 arg1Of lipopolysaccharide,induced
R6438 T7888 T7887 arg2Of lipopolysaccharide,by
R6439 T7888 T7889 arg1Of lipopolysaccharide,(
R6440 T7890 T7889 arg2Of LPS,(
R6441 T7891 T7889 arg3Of ),(
R6442 T7894 T7883 arg1Of attenuated,And
R6443 T7894 T7892 arg2Of attenuated,could
R6444 T7894 T7893 arg2Of attenuated,be
R6445 T7894 T7908 arg1Of attenuated,[
R6446 T7898 T7894 arg1Of activators,attenuated
R6447 T7898 T7895 arg2Of activators,by
R6448 T7898 T7896 arg1Of activators,diverse
R6449 T7898 T7897 arg1Of activators,Nrf2
R6450 T7898 T7899 arg1Of activators,","
R6451 T7898 T7901 arg1Of activators,as
R6452 T7901 T7900 arg1Of as,such
R6453 T7902 T7903 arg1Of SFN,and
R6454 T7903 T7901 arg2Of and,as
R6455 T7903 T7905 arg1Of and,(
R6456 T7904 T7903 arg2Of curcumin,and
R6457 T7906 T7905 arg2Of CUR,(
R6458 T7907 T7905 arg3Of ),(
R6459 T7909 T7908 arg2Of 22,[
R6460 T7910 T7908 arg3Of ],[
R6461 T7915 T7913 arg1Of studies,our
R6462 T7915 T7914 arg1Of studies,previous
R6463 T7915 T7916 arg1Of studies,indicate
R6464 T7916 T7911 arg1Of indicate,Furthermore
R6465 T7916 T7912 arg1Of indicate,","
R6466 T7918 T7919 arg1Of depletion,of
R6467 T7918 T7921 arg1Of depletion,induces
R6468 T7920 T7919 arg2Of Nrf2,of
R6469 T7921 T7916 arg2Of induces,indicate
R6470 T7921 T7917 arg1Of induces,that
R6471 T7922 T7921 arg2Of augmentation,induces
R6472 T7922 T7923 arg1Of augmentation,of
R6473 T7922 T7929 arg1Of augmentation,in
R6474 T7922 T7936 arg1Of augmentation,after
R6475 T7925 T7924 arg1Of activity,NF-κB
R6476 T7925 T7926 arg1Of activity,and
R6477 T7926 T7923 arg2Of and,of
R6478 T7928 T7926 arg2Of response,and
R6479 T7928 T7927 arg1Of response,inflammatory
R6480 T7930 T7931 arg1Of lung,","
R6481 T7931 T7934 arg1Of ",",and
R6482 T7932 T7931 arg2Of brain,","
R6483 T7934 T7929 arg2Of and,in
R6484 T7934 T7933 arg1Of and,","
R6485 T7935 T7934 arg2Of intestine,and
R6486 T7937 T7936 arg2Of TBI,after
R6487 T7937 T7938 arg1Of TBI,[
R6488 T7939 T7940 arg1Of 4,","
R6489 T7940 T7938 arg2Of ",",[
R6490 T7940 T7942 arg1Of ",",","
R6491 T7941 T7940 arg2Of 5,","
R6492 T7943 T7942 arg2Of 23,","
R6493 T7944 T7938 arg3Of ],[
R6494 T7945 T7946 arg1Of Results,from
R6495 T7945 T7950 arg1Of Results,confirmed
R6496 T7948 T7946 arg2Of study,from
R6497 T7948 T7947 arg1Of study,this
R6498 T7950 T7949 arg1Of confirmed,further
R6499 T7952 T7951 arg1Of relationship,such
R6500 T7952 T7953 arg1Of relationship,existed
R6501 T7953 T7950 arg2Of existed,confirmed
R6502 T7953 T7954 arg1Of existed,in
R6503 T7953 T7957 arg1Of existed,after
R6504 T7956 T7954 arg2Of astrocytes,in
R6505 T7956 T7955 arg2Of astrocytes,cultured
R6506 T7959 T7957 arg2Of injury,after
R6507 T7959 T7958 arg1Of injury,scratch
R6508 T7961 T7960 arg2Of activation,Enhanced
R6509 T7961 T7962 arg1Of activation,of
R6510 T7961 T7964 arg1Of activation,is
R6511 T7961 T7966 arg2Of activation,discovered
R6512 T7963 T7962 arg2Of NF-κB,of
R6513 T7966 T7964 arg2Of discovered,is
R6514 T7966 T7965 arg1Of discovered,also
R6515 T7966 T7967 arg1Of discovered,in
R6516 T7966 T7979 arg1Of discovered,after
R6517 T7966 T7982 arg1Of discovered,[
R6518 T7968 T7969 arg1Of lung,","
R6519 T7969 T7972 arg1Of ",",and
R6520 T7970 T7969 arg2Of macrophages,","
R6521 T7972 T7967 arg2Of and,in
R6522 T7972 T7971 arg1Of and,","
R6523 T7975 T7972 arg2Of fibroblasts,and
R6524 T7975 T7973 arg1Of fibroblasts,mouse
R6525 T7975 T7974 arg1Of fibroblasts,embryonic
R6526 T7975 T7976 arg1Of fibroblasts,of
R6527 T7978 T7976 arg2Of mice,of
R6528 T7978 T7977 arg1Of mice,Nrf2-deficient
R6529 T7981 T7979 arg2Of sepsis,after
R6530 T7981 T7980 arg1Of sepsis,experimental
R6531 T7983 T7982 arg2Of 24,[
R6532 T7984 T7982 arg3Of ],[
R6533 T7987 T7988 arg1Of it,is
R6534 T7987 T7989 arg2Of it,reported
R6535 T7989 T7985 arg1Of reported,Interestingly
R6536 T7989 T7986 arg1Of reported,","
R6537 T7989 T7988 arg2Of reported,is
R6538 T7991 T7992 arg1Of NF-κB,can
R6539 T7991 T7993 arg1Of NF-κB,inhibit
R6540 T7993 T7989 arg3Of inhibit,reported
R6541 T7993 T7990 arg1Of inhibit,that
R6542 T7993 T7992 arg2Of inhibit,can
R6543 T7993 T7995 arg1Of inhibit,at
R6544 T7994 T7993 arg2Of Nrf2,inhibit
R6545 T7997 T7995 arg2Of level,at
R6546 T7997 T7996 arg1Of level,transcriptional
R6547 T8000 T7998 arg1Of subunit,NF-κB
R6548 T8000 T7999 arg1Of subunit,p65
R6549 T8000 T8001 arg1Of subunit,repressed
R6550 T8001 T8008 arg1Of repressed,by
R6551 T8004 T8001 arg2Of pathway,repressed
R6552 T8004 T8002 arg1Of pathway,the
R6553 T8004 T8003 arg1Of pathway,Nrf2-ARE
R6554 T8004 T8005 arg1Of pathway,at
R6555 T8007 T8005 arg2Of level,at
R6556 T8007 T8006 arg1Of level,transcriptional
R6557 T8010 T8009 arg1Of interaction,competitive
R6558 T8010 T8011 arg1Of interaction,with
R6559 T8010 T8017 arg1Of interaction,or
R6560 T8014 T8011 arg2Of domain,with
R6561 T8014 T8012 arg1Of domain,the
R6562 T8014 T8013 arg1Of domain,CH1-KIX
R6563 T8014 T8015 arg1Of domain,of
R6564 T8016 T8015 arg2Of CBP,of
R6565 T8017 T8008 arg2Of or,by
R6566 T8020 T8017 arg2Of hypoacetylation,or
R6567 T8020 T8018 arg1Of hypoacetylation,local
R6568 T8020 T8019 arg1Of hypoacetylation,histone
R6569 T8020 T8021 arg1Of hypoacetylation,[
R6570 T8022 T8021 arg2Of 25,[
R6571 T8023 T8021 arg3Of ],[
R6572 T8026 T8024 arg1Of findings,All
R6573 T8026 T8025 arg1Of findings,these
R6574 T8026 T8027 arg1Of findings,indicate
R6575 T8031 T8027 arg2Of crosstalk,indicate
R6576 T8031 T8028 arg1Of crosstalk,the
R6577 T8031 T8029 arg1Of crosstalk,potential
R6578 T8031 T8030 arg1Of crosstalk,complicate
R6579 T8031 T8032 arg1Of crosstalk,between
R6580 T8033 T8034 arg1Of NF-κB,and
R6581 T8034 T8032 arg2Of and,between
R6582 T8034 T8036 arg1Of and,","
R6583 T8034 T8037 arg1Of and,which
R6584 T8034 T8038 arg1Of and,may
R6585 T8034 T8039 arg1Of and,be
R6586 T8034 T8040 arg2Of and,regulated
R6587 T8035 T8034 arg2Of Nrf2,and
R6588 T8040 T8038 arg2Of regulated,may
R6589 T8040 T8039 arg2Of regulated,be
R6590 T8040 T8051 arg1Of regulated,[
R6591 T8046 T8044 arg1Of kinase,mitogen-activated
R6592 T8046 T8045 arg1Of kinase,protein
R6593 T8046 T8047 arg1Of kinase,(
R6594 T8048 T8047 arg2Of MAPKs,(
R6595 T8049 T8047 arg3Of ),(
R6596 T8050 T8040 arg1Of pathway,regulated
R6597 T8050 T8041 arg2Of pathway,by
R6598 T8050 T8042 arg1Of pathway,the
R6599 T8050 T8043 arg1Of pathway,upstream
R6600 T8050 T8046 arg1Of pathway,kinase
R6601 T8052 T8051 arg2Of 26,[
R6602 T8053 T8051 arg3Of ],[
R6603 T8057 T8055 arg2Of confirmed,has
R6604 T8057 T8056 arg2Of confirmed,been
R6605 T8059 T8060 arg1Of Nrf2,is
R6606 T8059 T8062 arg2Of Nrf2,detected
R6607 T8062 T8054 arg1Of detected,It
R6608 T8062 T8055 arg1Of detected,has
R6609 T8062 T8056 arg1Of detected,been
R6610 T8062 T8057 arg2Of detected,confirmed
R6611 T8062 T8058 arg1Of detected,that
R6612 T8062 T8060 arg2Of detected,is
R6613 T8062 T8061 arg1Of detected,mainly
R6614 T8062 T8063 arg1Of detected,in
R6615 T8064 T8063 arg2Of nucleus,in
R6616 T8064 T8065 arg1Of nucleus,of
R6617 T8066 T8065 arg2Of astrocytes,of
R6618 T8066 T8067 arg1Of astrocytes,after
R6619 T8068 T8067 arg2Of TBI,after
R6620 T8068 T8069 arg1Of TBI,[
R6621 T8070 T8069 arg2Of 18,[
R6622 T8071 T8069 arg3Of ],[
R6623 T8073 T8072 arg2Of view,In
R6624 T8073 T8074 arg1Of view,of
R6625 T8076 T8074 arg2Of fact,of
R6626 T8076 T8075 arg1Of fact,the
R6627 T8078 T8079 arg1Of Nrf2,is
R6628 T8079 T8076 arg2Of is,fact
R6629 T8079 T8077 arg1Of is,that
R6630 T8082 T8079 arg2Of factor,is
R6631 T8082 T8080 arg1Of factor,a
R6632 T8082 T8081 arg1Of factor,transcription
R6633 T8082 T8083 arg1Of factor,which
R6634 T8082 T8084 arg1Of factor,should
R6635 T8082 T8085 arg1Of factor,take
R6636 T8085 T8084 arg2Of take,should
R6637 T8085 T8088 arg1Of take,in
R6638 T8086 T8085 arg2Of function,take
R6639 T8088 T8087 arg1Of in,mainly
R6640 T8089 T8088 arg2Of nucleus,in
R6641 T8091 T8092 arg1Of it,can
R6642 T8091 T8093 arg1Of it,be
R6643 T8091 T8094 arg2Of it,reasoned
R6644 T8094 T8072 arg1Of reasoned,In
R6645 T8094 T8090 arg1Of reasoned,","
R6646 T8094 T8092 arg2Of reasoned,can
R6647 T8094 T8093 arg2Of reasoned,be
R6648 T8096 T8097 arg1Of astrocytes,may
R6649 T8096 T8098 arg1Of astrocytes,be
R6650 T8098 T8094 arg3Of be,reasoned
R6651 T8098 T8095 arg1Of be,that
R6652 T8098 T8097 arg2Of be,may
R6653 T8098 T8104 arg1Of be,in
R6654 T8100 T8098 arg2Of kind,be
R6655 T8100 T8099 arg1Of kind,one
R6656 T8100 T8101 arg1Of kind,of
R6657 T8103 T8101 arg2Of cells,of
R6658 T8103 T8102 arg1Of cells,respondent
R6659 T8105 T8104 arg2Of activation,in
R6660 T8105 T8106 arg1Of activation,of
R6661 T8108 T8106 arg2Of pathway,of
R6662 T8108 T8107 arg1Of pathway,Nrf2-ARE
R6663 T8108 T8109 arg1Of pathway,after
R6664 T8110 T8109 arg2Of TBI,after
R6665 T8112 T8111 arg1Of study,Previous
R6666 T8112 T8113 arg1Of study,identified
R6667 T8113 T8126 arg1Of identified,after
R6668 T8115 T8113 arg2Of expression,identified
R6669 T8115 T8114 arg1Of expression,the
R6670 T8115 T8116 arg1Of expression,of
R6671 T8115 T8123 arg1Of expression,in
R6672 T8117 T8118 arg1Of TNF-α,","
R6673 T8118 T8121 arg1Of ",",and
R6674 T8119 T8118 arg2Of IL-1β,","
R6675 T8121 T8116 arg2Of and,of
R6676 T8121 T8120 arg1Of and,","
R6677 T8122 T8121 arg2Of IL-6,and
R6678 T8125 T8123 arg2Of astrocytes,in
R6679 T8125 T8124 arg2Of astrocytes,cultured
R6680 T8127 T8126 arg2Of treatment,after
R6681 T8127 T8128 arg1Of treatment,with
R6682 T8129 T8130 arg1Of LPS,or
R6683 T8130 T8128 arg2Of or,with
R6684 T8130 T8132 arg1Of or,[
R6685 T8131 T8130 arg2Of oxyhemoglobin,or
R6686 T8133 T8132 arg2Of 27,[
R6687 T8133 T8134 arg1Of 27,","
R6688 T8135 T8134 arg2Of 28,","
R6689 T8136 T8132 arg3Of ],[
R6690 T8138 T8137 arg1Of study,Another
R6691 T8138 T8139 arg1Of study,revealed
R6692 T8145 T8141 arg2Of occlusion,after
R6693 T8145 T8142 arg1Of occlusion,middle
R6694 T8145 T8143 arg1Of occlusion,cerebral
R6695 T8145 T8144 arg1Of occlusion,artery
R6696 T8148 T8147 arg1Of astrocytes,MMP9-positive
R6697 T8148 T8149 arg1Of astrocytes,were
R6698 T8148 T8150 arg2Of astrocytes,observed
R6699 T8150 T8139 arg2Of observed,revealed
R6700 T8150 T8140 arg1Of observed,that
R6701 T8150 T8141 arg1Of observed,after
R6702 T8150 T8146 arg1Of observed,","
R6703 T8150 T8149 arg2Of observed,were
R6704 T8150 T8151 arg1Of observed,in
R6705 T8150 T8154 arg1Of observed,by
R6706 T8150 T8156 arg1Of observed,[
R6707 T8153 T8151 arg2Of tissues,in
R6708 T8153 T8152 arg1Of tissues,brain
R6709 T8155 T8154 arg2Of immunohistochemistry,by
R6710 T8157 T8156 arg2Of 29,[
R6711 T8158 T8156 arg3Of ],[
R6712 T8160 T8159 arg1Of results,Those
R6713 T8160 T8161 arg1Of results,indicate
R6714 T8161 T8169 arg1Of indicate,after
R6715 T8163 T8161 arg2Of role,indicate
R6716 T8163 T8162 arg1Of role,the
R6717 T8163 T8164 arg1Of role,of
R6718 T8163 T8166 arg1Of role,in
R6719 T8165 T8164 arg2Of astrocytes,of
R6720 T8168 T8166 arg2Of process,in
R6721 T8168 T8167 arg1Of process,inflammatory
R6722 T8171 T8169 arg2Of injury,after
R6723 T8171 T8170 arg1Of injury,brain
R6724 T8174 T8177 modOf now,is
R6725 T8174 T8180 arg1Of now,focused
R6726 T8176 T8177 arg1Of there,is
R6727 T8177 T8175 arg1Of is,","
R6728 T8179 T8177 arg2Of study,is
R6729 T8179 T8178 arg1Of study,no
R6730 T8180 T8172 arg1Of focused,But
R6731 T8180 T8173 arg1Of focused,till
R6732 T8180 T8181 arg1Of focused,on
R6733 T8180 T8192 arg1Of focused,after
R6734 T8183 T8181 arg2Of relationship,on
R6735 T8183 T8182 arg1Of relationship,the
R6736 T8183 T8184 arg1Of relationship,among
R6737 T8185 T8186 arg1Of astrocytes,","
R6738 T8186 T8189 arg1Of ",",and
R6739 T8187 T8186 arg2Of Nrf2,","
R6740 T8189 T8184 arg2Of and,among
R6741 T8189 T8188 arg1Of and,","
R6742 T8191 T8189 arg2Of mediators,and
R6743 T8191 T8190 arg1Of mediators,proinflammatory
R6744 T8193 T8192 arg2Of TBI,after
R6745 T8195 T8194 arg1Of results,Our
R6746 T8195 T8196 arg1Of results,demonstrated
R6747 T8199 T8196 arg2Of expression,demonstrated
R6748 T8199 T8197 arg1Of expression,the
R6749 T8199 T8198 arg2Of expression,upregulated
R6750 T8199 T8200 arg1Of expression,of
R6751 T8199 T8209 arg1Of expression,in
R6752 T8201 T8202 arg1Of TNF-α,","
R6753 T8202 T8204 arg1Of ",",","
R6754 T8203 T8202 arg2Of IL-1β,","
R6755 T8204 T8207 arg1Of ",",and
R6756 T8205 T8204 arg2Of IL-6,","
R6757 T8207 T8200 arg2Of and,of
R6758 T8207 T8206 arg1Of and,","
R6759 T8208 T8207 arg2Of MMP9,and
R6760 T8212 T8209 arg2Of astrocytes,in
R6761 T8212 T8210 arg1Of astrocytes,Nrf2
R6762 T8212 T8211 arg1Of astrocytes,knockout
R6763 T8212 T8213 arg1Of astrocytes,after
R6764 T8215 T8213 arg2Of injury,after
R6765 T8215 T8214 arg1Of injury,scratch
R6766 T8215 T8216 arg1Of injury,for
R6767 T8219 T8216 arg2Of time,for
R6768 T8219 T8217 arg1Of time,the
R6769 T8219 T8218 arg1Of time,first
R6770 T8221 T8220 arg2Of conclusion,In
R6771 T8223 T8224 arg1Of depletion,of
R6772 T8223 T8226 arg1Of depletion,induced
R6773 T8225 T8224 arg2Of Nrf2,of
R6774 T8226 T8220 arg1Of induced,In
R6775 T8226 T8222 arg1Of induced,","
R6776 T8226 T8243 modOf induced,resulting
R6777 T8228 T8227 arg1Of activation,the
R6778 T8228 T8229 arg1Of activation,of
R6779 T8228 T8231 arg1Of activation,and
R6780 T8230 T8229 arg2Of NF-κB,of
R6781 T8231 T8226 arg2Of and,induced
R6782 T8233 T8231 arg2Of expression,and
R6783 T8233 T8232 arg1Of expression,the
R6784 T8233 T8234 arg1Of expression,of
R6785 T8235 T8236 arg1Of TNF-α,","
R6786 T8236 T8238 arg1Of ",",","
R6787 T8237 T8236 arg2Of IL-1β,","
R6788 T8238 T8241 arg1Of ",",and
R6789 T8239 T8238 arg2Of IL-6,","
R6790 T8241 T8234 arg2Of and,of
R6791 T8241 T8240 arg1Of and,","
R6792 T8242 T8241 arg2Of MMP9,and
R6793 T8243 T8244 arg1Of resulting,in
R6794 T8247 T8244 arg2Of deaths,in
R6795 T8247 T8245 arg1Of deaths,more
R6796 T8247 T8246 arg1Of deaths,cell
R6797 T8247 T8248 arg1Of deaths,in
R6798 T8249 T8248 arg2Of astrocytes,in
R6799 T8249 T8250 arg1Of astrocytes,after
R6800 T8252 T8250 arg2Of injury,after
R6801 T8252 T8251 arg1Of injury,scratch
R6802 T8254 T8253 arg1Of results,These
R6803 T8254 T8255 arg1Of results,suggest
R6804 T8257 T8258 arg1Of Nrf2,may
R6805 T8257 T8259 arg1Of Nrf2,be
R6806 T8259 T8255 arg2Of be,suggest
R6807 T8259 T8256 arg1Of be,that
R6808 T8259 T8258 arg2Of be,may
R6809 T8262 T8259 arg2Of target,be
R6810 T8262 T8260 arg1Of target,an
R6811 T8262 T8261 arg1Of target,important
R6812 T8262 T8263 arg1Of target,for
R6813 T8262 T8266 arg1Of target,after
R6814 T8265 T8263 arg2Of therapy,for
R6815 T8265 T8264 arg1Of therapy,anti-inflammatory
R6816 T8267 T8266 arg2Of TBI,after

bionlp-st-ge-2016-coref

Id Subject Object Predicate Lexical cue
T5454 12878-12882 Antecedent denotes IL-6
T5453 12867-12872 Antecedent denotes IL-1β
T5452 12978-13009 Anaphor denotes these proinflammatory cytokines
R4572 T5452 T5453 boundBy these proinflammatory cytokines,IL-1β
R4573 T5452 T5454 boundBy these proinflammatory cytokines,IL-6

bionlp-st-ge-2016-test-proteins

Id Subject Object Predicate Lexical cue
T7341 20569-20573 Protein denotes MMP9
T7340 20559-20563 Protein denotes IL-6
T7339 20552-20557 Protein denotes IL-1β
T7338 20384-20388 Protein denotes MMP9
T7337 20374-20378 Protein denotes IL-6
T7336 20367-20372 Protein denotes IL-1β
T7335 20004-20008 Protein denotes MMP9
T7334 19856-19860 Protein denotes IL-6
T5466 14354-14359 Protein denotes IL-1β
T5465 14243-14247 Protein denotes IL-6
T5464 14232-14237 Protein denotes IL-1β
T5463 14223-14227 Protein denotes IL-6
T5462 13849-13853 Protein denotes IL-6
T5461 13801-13806 Protein denotes IL-1β
T5460 13493-13497 Protein denotes IL-6
T5459 13482-13487 Protein denotes IL-1β
T5458 13360-13364 Protein denotes IL-6
T7333 19845-19850 Protein denotes IL-1β
T7332 19138-19141 Protein denotes p65
T7331 18365-18368 Protein denotes p65
T7330 17879-17883 Protein denotes MMP9
T7329 17869-17873 Protein denotes IL-6
T7328 17862-17867 Protein denotes IL-1β
T7327 17263-17267 Protein denotes HO-1
T7326 17245-17261 Protein denotes heme oxygenase-1
T7325 16616-16621 Protein denotes IL-1β
T7324 16560-16564 Protein denotes MMP9
T7323 16529-16533 Protein denotes IL-6
T7322 16496-16500 Protein denotes MMP9
T7321 16463-16468 Protein denotes IL-1β
T7320 16263-16267 Protein denotes MMP9
T7319 16104-16108 Protein denotes IL-6
T7318 16093-16098 Protein denotes IL-1β
T7317 16027-16031 Protein denotes MMP9
T7316 15583-15587 Protein denotes MMP9
T7315 15573-15577 Protein denotes IL-6
T7314 15566-15571 Protein denotes IL-1β
T3109 7817-7824 Protein denotes β-actin
T6500 15296-15300 Protein denotes MMP9
T6499 15134-15138 Protein denotes MMP9
T6498 14965-14969 Protein denotes MMP9
T6497 14842-14846 Protein denotes MMP9
T6496 14730-14734 Protein denotes MMP9
T6495 14573-14577 Protein denotes MMP9
T6494 14497-14501 Protein denotes MMP9
T4389 11367-11371 Protein denotes MMP9
T4388 11111-11115 Protein denotes MMP9
T4071 9690-9694 Protein denotes IL-6
T4070 9675-9688 Protein denotes interleukin-6
T4069 9663-9668 Protein denotes IL-1β
T4068 9643-9661 Protein denotes interleukin-1 beta
T3385 8743-8747 Protein denotes MMP9
T43 741-746 Protein denotes IL-1β
T44 748-752 Protein denotes IL-6
T45 758-762 Protein denotes MMP9
T46 843-847 Protein denotes MMP9
T47 1002-1006 Protein denotes MMP9
T5457 13349-13354 Protein denotes IL-1β
T5456 12878-12882 Protein denotes IL-6
T5455 12867-12872 Protein denotes IL-1β

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T8392 19260-19263 http://www.uniprot.org/uniprot/Q92793 denotes CBP
T8391 19138-19141 http://www.uniprot.org/uniprot/P21579 denotes p65
T8390 18365-18368 http://www.uniprot.org/uniprot/P21579 denotes p65
T8389 19138-19141 http://www.uniprot.org/uniprot/Q04206 denotes p65
T8388 18365-18368 http://www.uniprot.org/uniprot/Q04206 denotes p65
T8387 16520-16531 http://www.uniprot.org/uniprot/P35225 denotes 13], and IL
T8386 20559-20563 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T8385 20374-20378 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T8384 19856-19860 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T8383 17869-17873 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T8382 16529-16533 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T8381 16104-16108 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T8375 16616-16621 http://www.uniprot.org/uniprot/P01584 denotes IL-1β
T8374 16463-16468 http://www.uniprot.org/uniprot/P01584 denotes IL-1β
T8373 16093-16098 http://www.uniprot.org/uniprot/P01584 denotes IL-1β
T8372 15566-15571 http://www.uniprot.org/uniprot/P01584 denotes IL-1β
T8371 20545-20550 http://www.uniprot.org/uniprot/P01375 denotes TNF-α
T8370 20360-20365 http://www.uniprot.org/uniprot/P01375 denotes TNF-α
T8369 19838-19843 http://www.uniprot.org/uniprot/P01375 denotes TNF-α
T8368 17855-17860 http://www.uniprot.org/uniprot/P01375 denotes TNF-α
T8367 16606-16611 http://www.uniprot.org/uniprot/P01375 denotes TNF-α
T8366 16453-16458 http://www.uniprot.org/uniprot/P01375 denotes TNF-α
T8365 16086-16091 http://www.uniprot.org/uniprot/P01375 denotes TNF-α
T8364 15559-15564 http://www.uniprot.org/uniprot/P01375 denotes TNF-α
T8363 20360-20363 http://www.uniprot.org/uniprot/P01375 denotes TNF
T8362 19838-19841 http://www.uniprot.org/uniprot/P01375 denotes TNF
T8361 17855-17858 http://www.uniprot.org/uniprot/P01375 denotes TNF
T8360 16606-16609 http://www.uniprot.org/uniprot/P01375 denotes TNF
T8359 16453-16456 http://www.uniprot.org/uniprot/P01375 denotes TNF
T8358 16086-16089 http://www.uniprot.org/uniprot/P01375 denotes TNF
T8357 15559-15562 http://www.uniprot.org/uniprot/P01375 denotes TNF
T5869 14243-14247 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T5868 14223-14227 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T5867 13849-13853 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T5866 13493-13497 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T5865 13360-13364 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T5864 12878-12882 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T5863 14354-14359 http://www.uniprot.org/uniprot/P01584 denotes IL-1β
T5862 14232-14237 http://www.uniprot.org/uniprot/P01584 denotes IL-1β
T5861 13801-13806 http://www.uniprot.org/uniprot/P01584 denotes IL-1β
T5860 13482-13487 http://www.uniprot.org/uniprot/P01584 denotes IL-1β
T5859 13349-13354 http://www.uniprot.org/uniprot/P01584 denotes IL-1β
T5858 12867-12872 http://www.uniprot.org/uniprot/P01584 denotes IL-1β
T5857 13942-13947 http://www.uniprot.org/uniprot/P01375 denotes TNF-α
T5856 13757-13762 http://www.uniprot.org/uniprot/P01375 denotes TNF-α
T5855 13475-13480 http://www.uniprot.org/uniprot/P01375 denotes TNF-α
T5854 13342-13347 http://www.uniprot.org/uniprot/P01375 denotes TNF-α
T5853 12860-12865 http://www.uniprot.org/uniprot/P01375 denotes TNF-α
T5852 13757-13760 http://www.uniprot.org/uniprot/P01375 denotes TNF
T5851 13475-13478 http://www.uniprot.org/uniprot/P01375 denotes TNF
T5850 13342-13345 http://www.uniprot.org/uniprot/P01375 denotes TNF
T5849 12860-12863 http://www.uniprot.org/uniprot/P01375 denotes TNF
T4291 9687-9692 http://www.uniprot.org/uniprot/P05231 denotes 6 (IL
T4290 9663-9668 http://www.uniprot.org/uniprot/P01584 denotes IL-1β
T4289 9635-9638 http://www.uniprot.org/uniprot/P01375 denotes TNF
T3688 9328-9335 http://www.uniprot.org/uniprot/P60709 denotes β-actin
T3687 8644-8651 http://www.uniprot.org/uniprot/P60709 denotes β-actin
T265 734-739 http://www.uniprot.org/uniprot/P01375 denotes TNF-α
T266 741-746 http://www.uniprot.org/uniprot/P01584 denotes IL-1β
T267 748-752 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T8380 15573-15577 http://www.uniprot.org/uniprot/P05231 denotes IL-6
T8379 20552-20557 http://www.uniprot.org/uniprot/P01584 denotes IL-1β
T8378 20367-20372 http://www.uniprot.org/uniprot/P01584 denotes IL-1β
T8377 19845-19850 http://www.uniprot.org/uniprot/P01584 denotes IL-1β
T8376 17862-17867 http://www.uniprot.org/uniprot/P01584 denotes IL-1β
T4972 11672-11675 http://www.uniprot.org/uniprot/Q8IX06 denotes 3.1
T4971 11672-11675 http://www.uniprot.org/uniprot/Q5D1E8 denotes 3.1
T4970 11672-11675 http://www.uniprot.org/uniprot/Q5D1E7 denotes 3.1
T3226 7817-7824 http://www.uniprot.org/uniprot/P60709 denotes β-actin

UBERON-AE

Id Subject Object Predicate Lexical cue
T7213 19986-19992 http://purl.obolibrary.org/obo/UBERON_0001637 denotes artery
T7212 19977-19992 http://purl.obolibrary.org/obo/UBERON_0004449 denotes cerebral artery
T7211 19970-19992 http://purl.obolibrary.org/obo/UBERON_0001627 denotes middle cerebral artery
T7210 18746-18755 http://purl.obolibrary.org/obo/UBERON_0000160 denotes intestine
T7209 18942-18946 http://purl.obolibrary.org/obo/UBERON_0002048 denotes lung
T7208 18729-18733 http://purl.obolibrary.org/obo/UBERON_0002048 denotes lung
T7207 17577-17583 http://purl.obolibrary.org/obo/UBERON_0001851 denotes cortex
T7206 16574-16579 http://purl.obolibrary.org/obo/UBERON_0001155 denotes colon
T7205 17134-17140 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissue
T7204 16119-16126 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissues
T7203 15888-15899 http://purl.obolibrary.org/obo/UBERON_0002240 denotes spinal cord
T7202 20166-20171 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain
T7201 20046-20051 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain
T7200 18735-18740 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain
T7199 17783-17788 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain
T7198 17362-17367 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain
T7197 17128-17133 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain
T7196 16782-16787 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain
T7195 16113-16118 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain
T7194 15878-15883 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain
T5416 13161-13166 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain
T5105 12377-12382 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain
T3373 8559-8563 http://purl.obolibrary.org/obo/UBERON_0001913 denotes milk
T2308 6293-6298 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T1223 3458-3464 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissue
T1222 3306-3312 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissue
T1221 3280-3287 http://purl.obolibrary.org/obo/UBERON_0000055 denotes vessels
T1220 3274-3287 http://purl.obolibrary.org/obo/UBERON_0001981 denotes blood vessels
T1219 3274-3279 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood
T619 1880-1886 http://purl.obolibrary.org/obo/UBERON_0000062 denotes organs
T618 1554-1573 http://purl.obolibrary.org/obo/UBERON_0000120 denotes blood-brain barrier
T617 1621-1626 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood
T616 1554-1559 http://purl.obolibrary.org/obo/UBERON_0000178 denotes blood
T615 2470-2475 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain
T614 2118-2123 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain
T613 2096-2101 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain
T612 1856-1861 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain
T611 1581-1586 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain
T610 1560-1565 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain
T609 1307-1312 http://purl.obolibrary.org/obo/UBERON_0000955 denotes Brain
T23 234-239 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain

GO-BP

Id Subject Object Predicate Lexical cue
T7367 20592-20603 http://purl.obolibrary.org/obo/GO_0008219 denotes cell deaths
T7366 19743-19762 http://purl.obolibrary.org/obo/GO_0001775 denotes cells in activation
T7365 19437-19461 http://purl.obolibrary.org/obo/GO_0032111 denotes activated protein kinase
T7364 19437-19461 http://purl.obolibrary.org/obo/GO_0030295 denotes activated protein kinase
T7363 19437-19461 http://purl.obolibrary.org/obo/GO_0032147 denotes activated protein kinase
T7362 19437-19461 http://purl.obolibrary.org/obo/GO_0034199 denotes activated protein kinase
T7361 18345-18360 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T7360 18337-18340 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK
T7359 18437-18446 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T7358 18273-18282 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T7357 18431-18446 http://purl.obolibrary.org/obo/GO_0038061 denotes NF-κB signaling
T7356 18267-18282 http://purl.obolibrary.org/obo/GO_0038061 denotes NF-κB signaling
T7355 20597-20603 http://purl.obolibrary.org/obo/GO_0016265 denotes deaths
T7354 18088-18094 http://purl.obolibrary.org/obo/GO_0016265 denotes deaths
T7353 17459-17465 http://purl.obolibrary.org/obo/GO_0007613 denotes memory
T7352 17309-17313 http://purl.obolibrary.org/obo/GO_0003955 denotes NQO1
T7351 17250-17259 http://purl.obolibrary.org/obo/GO_0016701 denotes oxygenase
T7350 18704-18725 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammatory response
T7349 16737-16749 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T7348 19611-19624 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T7347 17190-17203 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T7346 16010-16023 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T7345 20503-20522 http://purl.obolibrary.org/obo/GO_0051092 denotes activation of NF-κB
T7344 18900-18919 http://purl.obolibrary.org/obo/GO_0051092 denotes activation of NF-κB
T7343 16638-16657 http://purl.obolibrary.org/obo/GO_0051092 denotes activation of NF-κB
T5112 12548-12567 http://purl.obolibrary.org/obo/GO_0051092 denotes activation of NF-κB
T5111 12355-12373 http://purl.obolibrary.org/obo/GO_0051092 denotes NF-κB is activated
T4855 12139-12144 http://purl.obolibrary.org/obo/GO_0016265 denotes death
T4854 11761-11766 http://purl.obolibrary.org/obo/GO_0016265 denotes death
T4853 11715-11720 http://purl.obolibrary.org/obo/GO_0016265 denotes Death
T4852 12134-12144 http://purl.obolibrary.org/obo/GO_0008219 denotes cell death
T4851 11756-11766 http://purl.obolibrary.org/obo/GO_0008219 denotes Cell death
T4850 11710-11720 http://purl.obolibrary.org/obo/GO_0008219 denotes Cell Death
T3114 7522-7535 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T3113 7514-7535 http://purl.obolibrary.org/obo/GO_0001171 denotes Reverse transcription
T3112 7319-7321 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T3111 7408-7410 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T3110 7264-7266 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T2046 5337-5339 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T2045 5037-5042 http://purl.obolibrary.org/obo/GO_0016265 denotes death
T1820 4585-4594 http://purl.obolibrary.org/obo/GO_0009058 denotes formation
T2044 4851-4856 http://purl.obolibrary.org/obo/GO_0016265 denotes Death
T2043 5032-5042 http://purl.obolibrary.org/obo/GO_0008219 denotes cell death
T2042 4846-4856 http://purl.obolibrary.org/obo/GO_0008219 denotes Cell Death
T48 45-57 http://purl.obolibrary.org/obo/GO_0006954 denotes Inflammation
T7342 15856-15874 http://purl.obolibrary.org/obo/GO_0051092 denotes NF-κB is activated
T6502 14781-14783 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T6501 14632-14644 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T5467 13420-13422 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T4391 11325-11330 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T4390 10607-10612 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T4080 10413-10415 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T4079 9825-9827 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T4078 9655-9665 http://purl.obolibrary.org/obo/GO_0032611 denotes 1 beta (IL
T4077 9643-9661 http://purl.obolibrary.org/obo/GO_0050702 denotes interleukin-1 beta
T4076 9643-9661 http://purl.obolibrary.org/obo/GO_0050720 denotes interleukin-1 beta
T4075 9612-9620 http://purl.obolibrary.org/obo/GO_0070265 denotes necrosis
T4074 9612-9620 http://purl.obolibrary.org/obo/GO_0019835 denotes necrosis
T4073 9612-9620 http://purl.obolibrary.org/obo/GO_0008219 denotes necrosis
T4072 9612-9620 http://purl.obolibrary.org/obo/GO_0001906 denotes necrosis
T3388 8785-8787 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T3387 8690-8692 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T3386 8402-8409 http://purl.obolibrary.org/obo/GO_0051923 denotes sulfate
T1239 4227-4229 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T1238 3443-3452 http://purl.obolibrary.org/obo/GO_0007586 denotes digestion
T639 1949-1962 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T638 1627-1648 http://purl.obolibrary.org/obo/GO_0001775 denotes cells, and activation
T637 1621-1648 http://purl.obolibrary.org/obo/GO_1903708 denotes blood cells, and activation
T636 1541-1550 http://purl.obolibrary.org/obo/GO_0009056 denotes breakdown
T635 2643-2655 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T634 2273-2285 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T633 2076-2088 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T632 1805-1817 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T631 1508-1529 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammatory response
T49 1193-1205 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T50 207-226 http://purl.obolibrary.org/obo/GO_0051092 denotes activation of NF-κB
T51 466-485 http://purl.obolibrary.org/obo/GO_0051092 denotes activation of NF-κB
T52 1214-1233 http://purl.obolibrary.org/obo/GO_0051092 denotes activation of NF-κB

GO-MF

Id Subject Object Predicate Lexical cue
T8295 19437-19461 http://purl.obolibrary.org/obo/GO_0030295 denotes activated protein kinase
T8294 19260-19263 http://purl.obolibrary.org/obo/GO_0008140 denotes CBP
T8293 18337-18340 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK
T6700 14781-14783 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T5836 13420-13422 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T5835 14243-14247 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T5834 14223-14227 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T5833 13849-13853 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T5832 13493-13497 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T5831 13360-13364 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T5830 12878-12882 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T4284 10413-10415 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T4283 9825-9827 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T4282 9687-9692 http://purl.obolibrary.org/obo/GO_0005138 denotes 6 (IL
T3674 9044-9052 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T3673 8816-8824 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T3672 8617-8627 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T2665 7201-7212 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA binding
T2664 7205-7212 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T2663 6650-6657 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T257 650-661 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA-binding
T258 654-661 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T259 748-752 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T8287 20374-20378 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T8286 19856-19860 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T8285 17869-17873 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T8284 16529-16533 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T8283 16104-16108 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T8282 15573-15577 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T8281 16227-16234 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T8280 15520-15527 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T8279 16223-16234 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA binding
T8278 15516-15527 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA binding
T3676 8785-8787 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T3675 8690-8692 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T1500 4227-4229 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T8292 17309-17313 http://purl.obolibrary.org/obo/GO_0003955 denotes NQO1
T8291 17250-17259 http://purl.obolibrary.org/obo/GO_0016701 denotes oxygenase
T8290 16520-16531 http://purl.obolibrary.org/obo/GO_0005144 denotes 13], and IL
T8289 20559-20563 http://purl.obolibrary.org/obo/GO_0005138 denotes IL-6
T5214 12423-12430 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T5213 12419-12430 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA-binding
T3224 7319-7321 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T3223 7408-7410 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T3222 7264-7266 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T2163 5337-5339 http://purl.obolibrary.org/obo/GO_0033968 denotes CA

GO-CC

Id Subject Object Predicate Lexical cue
T8300 19669-19676 http://purl.obolibrary.org/obo/GO_0005634 denotes nucleus
T8299 19538-19545 http://purl.obolibrary.org/obo/GO_0005634 denotes nucleus
T4969 11864-11875 http://purl.obolibrary.org/obo/GO_0009504 denotes plate, cell
T4968 12134-12138 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T4967 11871-11875 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T4966 11756-11760 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T4965 11710-11714 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T2168 5059-5063 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T2167 5032-5036 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T8298 20592-20596 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T8297 19743-19748 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T8296 16590-16595 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3685 9044-9052 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T3684 8816-8824 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T3683 8617-8627 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T3682 9044-9052 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T3681 8816-8824 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T3680 8617-8627 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T3679 8517-8526 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T3678 8487-8495 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T3677 7911-7916 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2670 6928-6945 http://purl.obolibrary.org/obo/GO_0043234 denotes protein complexes
T2669 6924-6945 http://purl.obolibrary.org/obo/GO_0097522 denotes DNA-protein complexes
T2668 6924-6945 http://purl.obolibrary.org/obo/GO_0032993 denotes DNA-protein complexes
T2667 6924-6945 http://purl.obolibrary.org/obo/GO_0001114 denotes DNA-protein complexes
T2666 5596-5600 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T1503 4051-4056 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T1502 3787-3792 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T1501 3771-3776 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T906 1677-1682 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T905 1627-1632 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2166 4985-4989 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T2165 4897-4902 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2164 4846-4850 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T1920 4644-4649 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T1919 4504-4508 http://purl.obolibrary.org/obo/GO_0005623 denotes cell

sentences

Id Subject Object Predicate Lexical cue
T24 0-119 Sentence denotes The Absence of Nrf2 Enhances NF-κB-Dependent Inflammation following Scratch Injury in Mouse Primary Cultured Astrocytes
T7247 20640-20739 Sentence denotes These results suggest that Nrf2 may be an important target for anti-inflammatory therapy after TBI.
T7246 20458-20639 Sentence denotes In conclusion, depletion of Nrf2 induced the activation of NF-κB and the expression of TNF-α, IL-1β, IL-6, and MMP9 resulting in more cell deaths in astrocytes after scratch injury.
T7245 20305-20457 Sentence denotes Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9 in Nrf2 knockout astrocytes after scratch injury for the first time.
T7244 20180-20304 Sentence denotes But till now, there is no study focused on the relationship among astrocytes, Nrf2, and proinflammatory mediators after TBI.
T7243 20090-20179 Sentence denotes Those results indicate the role of astrocytes in inflammatory process after brain injury.
T7242 19936-20089 Sentence denotes Another study revealed that after middle cerebral artery occlusion, MMP9-positive astrocytes were observed in brain tissues by immunohistochemistry [29].
T7241 19794-19935 Sentence denotes Previous study identified the expression of TNF-α, IL-1β, and IL-6 in cultured astrocytes after treatment with LPS or oxyhemoglobin [27, 28].
T7240 19576-19793 Sentence denotes In view of the fact that Nrf2 is a transcription factor which should take function mainly in nucleus, it can be reasoned that astrocytes may be one kind of respondent cells in activation of Nrf2-ARE pathway after TBI.
T7239 19484-19575 Sentence denotes It has been confirmed that Nrf2 is mainly detected in nucleus of astrocytes after TBI [18].
T7238 19303-19483 Sentence denotes All these findings indicate the potential complicate crosstalk between NF-κB and Nrf2, which may be regulated by the upstream mitogen-activated protein kinase (MAPKs) pathway [26].
T7237 19132-19302 Sentence denotes NF-κB p65 subunit repressed the Nrf2-ARE pathway at transcriptional level by competitive interaction with the CH1-KIX domain of CBP or local histone hypoacetylation [25].
T7236 19048-19131 Sentence denotes Interestingly, it is reported that NF-κB can inhibit Nrf2 at transcriptional level.
T7235 18891-19047 Sentence denotes Enhanced activation of NF-κB is also discovered in lung, macrophages, and mouse embryonic fibroblasts of Nrf2-deficient mice after experimental sepsis [24].
T7234 18778-18890 Sentence denotes Results from this study further confirmed such relationship existed in cultured astrocytes after scratch injury.
T7233 18595-18777 Sentence denotes Furthermore, our previous studies indicate that depletion of Nrf2 induces augmentation of NF-κB activity and inflammatory response in lung, brain, and intestine after TBI [4, 5, 23].
T7232 18453-18594 Sentence denotes And NF-κB activation induced by lipopolysaccharide (LPS) could be attenuated by diverse Nrf2 activators, such as SFN and curcumin (CUR) [22].
T7231 18284-18452 Sentence denotes Administration of SFN is found to be able to inhibit IKK/IκB phosphorylation and p65 NF-κB subunit nuclear translocation, consequently alleviating NF-κB signaling [21].
T7230 18096-18283 Sentence denotes Data obtained from animal studies suggest the possibility that antioxidant effect of Nrf2 may be achieved by suppression of proinflammatory pathways which are mediated by NF-κB signaling.
T7229 17805-18095 Sentence denotes Here, our results revealed that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch injury was more aggravated in cultured Nrf2 knockout astrocytes than in wildtype astrocyetes for the first time, and overexpression of these proinflammatory mediators led to more astrocytes deaths.
T7228 17707-17804 Sentence denotes Depletion of Nrf2 induces higher expression of proinflammatory mediators in brain after TBI [20].
T7227 17636-17706 Sentence denotes On the other hand, Nrf2-deficient mice appear more susceptible to TBI.
T7226 17374-17635 Sentence denotes Postinjury administration of SFN, an inducer of Nrf2, significantly improves spatial memory of rat and decreases the immunoreactivity for 4-Hydroxynonenal (4-HNE), a marker of lipid peroxidation, in the cortex and the CA3 subfield of hippocampus after TBI [19].
T7225 17157-17373 Sentence denotes Enhanced level of Nrf2 activates transcription of a group of antioxidant genes, such as heme oxygenase-1 (HO-1) and NAD (P)H: quinone oxidoreductase-1 (NQO1), which would subsequently reduce the damage in brain [18].
T7224 17070-17156 Sentence denotes Our previous study has proved the augmentation of Nrf2 in brain tissue after TBI [17].
T7223 16892-17069 Sentence denotes Nrf2-ARE pathway has been proved to be the key regulator in reducing oxidative stress, inflammatory damage, and accumulation of toxic metabolites, which are all involved in TBI.
T7222 16789-16891 Sentence denotes It is a good choice for targeting on an upstream factor to prevent such autoregulatory loop after TBI.
T7221 16668-16788 Sentence denotes This autoregulatory loop extremely aggravates the damaging effect of inflammation and induces secondary injury to brain.
T7220 16440-16667 Sentence denotes For example, TNF-α and IL-1β are potent stimulators for MMP9 in astrocytes [12, 13], and IL-6 induces overexpression of MMP9 in human colon carcinoma cells [14], and TNF-α and IL-1β also can induce activation of NF-κB [15, 16].
T7219 16339-16439 Sentence denotes It has been demonstrated that there is an autoregulatory loop among these proinflammatory mediators.
T7218 16150-16338 Sentence denotes Our results also revealed that scratch injury induced elevation of NF-κB DNA binding activity, overexpression of MMP9, and proinflammatory mediators mentioned above in cultured astrocytes.
T7217 15931-16149 Sentence denotes As a transcript factor, NF-κB binds with DNA once it was activated and induces transcription of MMP9 and a battery of proinflammatory cytokines, including TNF-α, IL-1β, and IL-6, in brain tissues after TBI [3, 10, 11].
T7216 15826-15930 Sentence denotes It has been demonstrated that NF-κB is activated in brain and spinal cord after traumatic injury [2, 9].
T7215 15612-15825 Sentence denotes Also we revealed that scratch injury induced higher activity of NF-κB and enhanced expression of proinflammatory cytokines in Nrf2 knockout cultured astrocytes than those in wildtype astrocytes for the first time.
T7214 15431-15611 Sentence denotes The present study demonstrated that scratch injury induced the upregulation of NF-κB DNA binding activity and overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes.
T6480 15260-15415 Sentence denotes Gelatine zymography revealed higher MMP9 activity in group KO TBI than that in group WT TBI too (101.76 ± 6.343 versus 76.32 ± 3.388, P < 0.01) (Figure 6).
T6479 15088-15259 Sentence denotes Significant difference was also discovered in MMP9 protein level by western blot, as 1.44 ± 0.076 for group KO TBI and 1.13 ± 0.048 for group WT TBI (P < 0.01) (Figure 5).
T6478 14947-15087 Sentence denotes The mRNA level of MMP9 was higher in group KO TBI than in group WT TBI (1.36 ± 0.090 versus 1.01 ± 0.068, P < 0.01) (Figures 3(a) and 3(e)).
T6477 14828-14946 Sentence denotes Expression of MMP9 was elevated after scratch injury in WT and KO astrocytes as compared with their sham counterparts.
T6476 14654-14827 Sentence denotes In the present study, we elevated the mRNA, protein levels, and activity of MMP9 in astrocytes at 24 h after scratch injury by RT-PCR, western blot, and gelatine zymography.
T6475 14573-14653 Sentence denotes MMP9 is an important gelatinase to induce or aggravate the inflammation process.
T6474 14470-14572 Sentence denotes Expression and Activity of MMP9 Were Greatly Enhanced in Nrf2 Knockout Astrocytes after Scratch Injury
T6473 14465-14469 Sentence denotes 3.4.
T5431 14350-14463 Sentence denotes For IL-1β, it was 190.75 ± 6.339 for group KO TBI while 154.50 ± 5.348 for group WT TBI (P < 0.01) (Figure 4(c)).
T5430 14239-14349 Sentence denotes For IL-6, it was 36.07 ± 0.786 for group KO TBI while 25.76 ± 0.536 for group WT TBI (P < 0.01) (Figure 4(b)).
T5429 14173-14238 Sentence denotes Similar tendency was observed in protein level of IL-6 and IL-1β.
T5428 14053-14172 Sentence denotes And it was also higher in group KO TBI than in group WT TBI (3.67 ± 0.156 versus 2.31 ± 0.087, P < 0.01) (Figure 4(a)).
T5427 13886-14052 Sentence denotes The results of ELISA revealed that the protein level of TNF-α was upregulated after scratch injury in WT and KO astrocytes when compared with their sham counterparts.
T5426 13855-13885 Sentence denotes P < 0.01) (Figures 3(a)–3(d)).
T5425 13614-13854 Sentence denotes Moreover, the mRNA levels of these cytokines in group KO TBI were much higher than those in group WT TBI (1.66 ± 0.085 versus 1.39 ± 0.110 for TNF-α, 1.34 ± 0.064 versus 0.73 ± 0.088 for IL-1β, and 1.28 ± 0.102 versus 0.99 ± 0.073 for IL-6.
T5424 13438-13613 Sentence denotes It was shown that the mRNA levels of TNF-α, IL-1β, and IL-6 were increased after scratch in both WT and KO astrocytes when compared with their sham counterparts, respectively.
T5423 13312-13437 Sentence denotes Here, we tested the levels of TNF-α, IL-1β, and IL-6 in cultured astrocytes at 24 h after scratch injury by RT-PCR and ELISA.
T5422 13205-13311 Sentence denotes But it is still ambiguous about the relationship between these cytokines and Nrf2 in astrocytes after TBI.
T5421 13053-13204 Sentence denotes And our previous study has proved that higher levels of such proinflammatory cytokines were observed in the brain of Nrf2-deficient mice after TBI [5].
T5420 12952-13052 Sentence denotes It has been revealed that these proinflammatory cytokines are increased after TBI in animals [2, 8].
T5419 12844-12951 Sentence denotes As we all know, TNF-α, IL-1β, and IL-6 are regulated by NF-κB and reflect the endogenous activity of NF-κB.
T5418 12742-12843 Sentence denotes Expression of Proinflammatory Cytokines Was Elevated in Nrf2 Knockout Astrocytes after Scratch Injury
T5417 12737-12741 Sentence denotes 3.3.
T5110 12525-12735 Sentence denotes Scratch injury induced activation of NF-κB in astrocytes of both genotypes, while higher NF-κB activity was observed in group KO TBI than in group WT TBI (2.67 ± 0.173 versus 2.28 ± 0.072, P < 0.01) (Figure 2).
T5109 12398-12524 Sentence denotes Here, we studied the DNA-binding activity of NF-κB of astrocytes from Nrf2 WT or KO mice by EMSA at 24 h after scratch injury.
T5108 12329-12397 Sentence denotes It has been reported that NF-κB is activated in brain after TBI [2].
T5107 12237-12328 Sentence denotes Disruption of Nrf2 Enhanced Upregulation of NF-κB DNA-Binding Activity after Scratch Injury
T5106 12232-12236 Sentence denotes 3.2.
T4849 12173-12230 Sentence denotes There was no difference between two sham-operated groups.
T4848 11985-12172 Sentence denotes CCK-8 assay also showed lower OD value of group KO TBI (0.38 ± 0.064) than that of group WT TBI (0.98 ± 0.098) (P < 0.01), which also suggested more cell death in group KO TBI (Figure 1).
T4847 11836-11984 Sentence denotes The detachment from culture plate, cell lost, and cytoplasmic process distortion were mostly observed in both sides of scratch line in group KO TBI.
T4846 11756-11835 Sentence denotes Cell death was detected by microscope and CCK-8 analysis at 24 h after scratch.
T4845 11677-11755 Sentence denotes Depletion of Nrf2 Aggravated the Cell Death in Astrocytes after Scratch Injury
T4844 11672-11676 Sentence denotes 3.1.
T4761 11602-11658 Sentence denotes All analyses were performed by using SPSS 18.0 software.
T4760 11552-11601 Sentence denotes P values <0.05 were considered to be significant.
T4759 11462-11551 Sentence denotes Data were expressed as mean ± SD and evaluated by ANOVA and LSD multiple comparison test.
T4758 11441-11461 Sentence denotes Statistical Analysis
T4757 11436-11440 Sentence denotes 2.9.
T4385 11349-11434 Sentence denotes Quantification of MMP9 band density was performed with image analysis program ImageJ.
T4384 11243-11348 Sentence denotes Proteolytic activities were showed by clear bands in blue gel which indicates the lysis of the substrate.
T4383 11139-11242 Sentence denotes After rinsing in water, each gel was stained with Coomassie blue for 1 h and destained in 50% methanol.
T4382 10973-11138 Sentence denotes Then the proteins were renatured by incubation in 2.5% Triton X-100 and then incubated in substrate buffer for 40 h at 37°C to enable the MMP9 to cleave the gelatin.
T4381 10896-10972 Sentence denotes Briefly, 40 μg cytosolic protein extracts were separated by electrophoresis.
T4380 10566-10895 Sentence denotes Cells of four groups were homogenized in lysis buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 5 mM CaCl2, 0.2 mM NaN3, and 0.01% Triton. Soluble extracts were separated by centrifugation and stored at –20°C. Gelatin zymography was performed according to the manufacturer's instructions (Genmed Scientifics Inc, MA, USA).
T4379 10546-10565 Sentence denotes Gelatine Zymography
T4378 10541-10545 Sentence denotes 2.8.
T4063 10423-10539 Sentence denotes The concentration of protein was determined according to the standard curve and expressed as pg/mg of total protein.
T4062 10306-10422 Sentence denotes The colour intensity was measured at 450 nm using a Bio-Rad ELISA microplate reader (Bio-Rad Laboratories, CA, USA).
T4061 10240-10305 Sentence denotes After incubated for about 10–30 min, the stop solution was added.
T4060 10144-10239 Sentence denotes After incubated for 1 h, microwell strips were washed 3 times followed by adding TMB substrate.
T4059 9994-10143 Sentence denotes After incubated for 2 h at room temperature, the microwell strips were washed 3 times with wash buffer, and streptavidin-HRP were added to all wells.
T4058 9929-9993 Sentence denotes Then samples and biotinconjugate were added to microwell strips.
T4057 9835-9928 Sentence denotes Briefly, prepared the standard and created the standard dilution for building standard curve.
T4056 9596-9834 Sentence denotes Levels of tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6) protein were quantified using ELISA kits specific for mouse according to the manufacturer's instructions (Bender MedSystems Inc. CA, USA).
T4055 9473-9595 Sentence denotes The supernatant was collected, and total protein was determined by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA).
T4054 9415-9472 Sentence denotes Cells of four groups were homogenized as mentioned above.
T4053 9373-9414 Sentence denotes Enzyme-Linked Immunosorbent Assay (ELISA)
T4052 9368-9372 Sentence denotes 2.7.
T3107 7804-7858 Sentence denotes The level of β-actin was used as an internal standard.
T3106 7745-7803 Sentence denotes The intensity of the bands was analyzed by ImageJ program.
T3105 7686-7744 Sentence denotes PCR products were detected by agarose gel electrophoresis.
T3104 7640-7685 Sentence denotes Table 1 shows the primers and PCR parameters.
T3103 7484-7638 Sentence denotes The cDNA was stored in −20°C. Reverse transcription was conducted with GoTaq Green Master Mix (Promega, WI, USA) according to the manufacturer's protocol.
T3102 7271-7483 Sentence denotes Total RNA was isolated with Trizol (Invitrogen, CA, USA), and single-stranded cDNA was synthesized from 2 μg of total RNA with BU-Script RT-Kit (Biunique, Jiangsu, China) according to the manufacturer's protocol.
T3101 7264-7270 Sentence denotes RT-PCR
T3100 7259-7263 Sentence denotes 2.5.
T3383 9315-9366 Sentence denotes The level of β-actin was used as internal standard.
T3382 9260-9314 Sentence denotes ImageJ was used to analyze the intensity of the blots.
T3381 9085-9259 Sentence denotes Blots were incubated with enhanced chemiluminescence (ECL) detection system (Amersham Biosciences, Bucks, UK) and exposed to radiographic film (Fuji Hyperfilm, Tokyo, Japan).
T3380 8927-9084 Sentence denotes The blots were washed three times in the washing buffer and covered with the horseradish peroxidase-linked secondary antibody at a 1 : 2000 dilution for 1 h.
T3379 8803-8926 Sentence denotes Each primary antibody was diluted appropriately in blocking buffer and then added to the blots for 1 h at room temperature.
T2322 7185-7257 Sentence denotes Levels of NF-κB DNA binding activity were quantified by software ImageJ.
T2321 6920-7184 Sentence denotes The DNA-protein complexes were separated by electrophoresis on 4% nondenaturing polyacrylamide gel in 0.5 × TBE buffer (tris-borate-EDTA) at 390 V for 1 hour at 4°C. After electrophoresis, the gel was dried and exposed to X-ray film (Fuji Hyperfilm, Tokyo, Japan).
T2320 6807-6919 Sentence denotes After addition of the 1 μL 32P-labled oligonucleotide probe, the incubation was continued for 20 minutes on ice.
T2319 6600-6806 Sentence denotes Nuclear protein (20 μg) was preincubated in 20 μL binding buffer containing 10 mM Tris-HCl (PH 7.5), 1 mM MgCl2, 0.5 mM NaCl, 4% glycerol, 0.5 mM EDTA, 0.5 mM DTT, and 2 μg poly dI-dC for 20 minutes on ice.
T2318 6442-6599 Sentence denotes Consensus oligonucleotide probe (5′-AGTTGAGGGGACTTTCCCAGGG-3′) was end-labeled by T4-polynucleotide kinase using [γ-32P]-ATP (Free Biotech., Beijing, China).
T2317 6365-6441 Sentence denotes EMSA was performed using gel shift assay system (Promega, Madison, WI, USA).
T2316 6207-6364 Sentence denotes Protein concentration was determined using a bicinchoninic acid assay kit with bovine serum albumin as the standard (Pierce Biochemicals, Rockford, IL, USA).
T2315 6061-6206 Sentence denotes Insoluble material was removed by centrifugation at 14000 rpm, and the supernatant containing the nuclear proteins was stored at –80°C until use.
T2314 5887-6060 Sentence denotes The nuclear proteins were extracted with buffer containing 20 mM HEPES (pH 7.9), 0.4 mM NaCl, 1.0 mM EDTA, 1.0 mM EGTA, 1.0 mM DTT, and 1.0 mM phenylmethylsulfonyl fluoride.
T2313 5785-5886 Sentence denotes Then 10% Nonidet P-40 was added and vortexed briefly, and the nuclei were pelleted by centrifugation.
T2312 5592-5784 Sentence denotes The cell pellet obtained by centrifugation was resuspended in buffer containing 10 mM HEPES (pH 7.9), 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 1.0 mM DTT, and 0.5 mM phenylmethylsulfonyl fluoride.
T2311 5505-5591 Sentence denotes Monolayers of astrocytes were washed with PBS and harvested by scraping into cold PBS.
T2310 5461-5504 Sentence denotes Electrophoretic Mobility Shift Assay (EMSA)
T2309 5456-5460 Sentence denotes 2.4.
T1819 4749-4839 Sentence denotes Cells, which did not received scratch, were used as blank control and named as sham group.
T1818 4617-4748 Sentence denotes Immediately after scratch, cells were washed twice with sterile PBS, cultured with complete culture medium, and named as TBI group.
T1817 4500-4616 Sentence denotes The cell monolayer was scratched with a sterile 26G syringe needle, resulting in the formation of a 0.5 mm wide gap.
T1816 4434-4499 Sentence denotes Astrocytes were planted in 6-well plates and grown to confluence.
T1815 4366-4433 Sentence denotes Astrocyte scratch injury was performed as in a previous report [7].
T1814 4314-4365 Sentence denotes In Vitro Model of TBI Established by Scratch Injury
T1813 4309-4313 Sentence denotes 2.2.
T2041 5396-5454 Sentence denotes Results were expressed as mean of OD value at 450 nm ± SD.
T2040 5347-5395 Sentence denotes All measurements were performed in sextuplicate.
T2039 5241-5346 Sentence denotes Then OD value was read at 450 nm using a Bio-Rad ELISA microplate reader (Bio-Rad Laboratories, CA, USA).
T2038 5171-5240 Sentence denotes Briefly, 10 μL CCK-8 was added into every well and incubated for 1 h.
T2037 5022-5170 Sentence denotes Astrocyte cell death was assessed by cell counting kit-8 (CCK-8) (Dojindo, Japan) assay 24 h after scratch according to the manufacturer's protocol.
T2036 4976-5021 Sentence denotes Then the cell was scrated as mentioned above.
T2035 4884-4975 Sentence denotes 1 × 103/well cells were seeded to 96-well culture plates and cultivated for 24 h to adhere.
T2034 4846-4883 Sentence denotes Cell Death Measurement by CCK-8 Assay
T2033 4841-4845 Sentence denotes 2.3.
T34 1119-1289 Sentence denotes These results suggest that the absence of Nrf2 may induce more aggressive inflammation through activation of NF-κB and downstream proinflammatory cytokines in astrocytes.
T33 1027-1118 Sentence denotes And such tendency was much more prominent in Nrf2 KO astrocytes than that in WT astrocytes.
T32 971-1026 Sentence denotes The expression and activity of MMP9 were also elevated.
T31 849-970 Sentence denotes The activity of NF-κB and expression of proinflammatory cytokines mentioned above were upregulated at 24 h after scratch.
T30 784-848 Sentence denotes Gelatin zymography was performed to detect the activity of MMP9.
T29 703-783 Sentence denotes The mRNA and protein levels of TNF-α, IL-1β, IL-6, and MMP9 were also evaluated.
T28 641-702 Sentence denotes Then the DNA-binding activity of NF-κB was evaluated by EMSA.
T27 558-640 Sentence denotes Primary cultured astrocytes were scratched to mimic the traumatic injury in vitro.
T26 342-557 Sentence denotes Hence, we used primary cultured astrocytes from either Nrf2 wildtype or knockout mice to study the influence of Nrf2 on the activation of NF-κB and expression of proinflammatory cytokines in a model of TBI in vitro.
T25 130-341 Sentence denotes It has been proved that Nrf2 depletion enhances inflammatory process through activation of NF-κB in the brain after TBI, but little is known about the relationship between Nrf2 and NF-κB in astrocytes after TBI.
T3378 8497-8802 Sentence denotes For immunoblotting, membranes were blocked with 5% nonfat dry milk in saline buffer overnight at 4°C, and the following antibodies were used: anti-β-actin (sc-130657, Santa Cruz Biotechnology, CA, USA, 43 kDa) and anti-matrix metallopeptidase 9 (MMP9) (sc-6841, Santa Cruz Biotechnology, CA, USA, 92 kDa).
T3377 8255-8496 Sentence denotes Fifty micrograms of the resulting cytosolic protein extracts were heat-denatured in Laemmli sample loading buffer, separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis, and electroblotted onto a nitrocellulose membrane.
T3376 7878-8254 Sentence denotes To obtain total protein lysates, cells were homogenized in RIPA buffer (1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, 20 mM NaF, 0.5 mM DTT, 1 mM PMSF, and protease inhibitor cocktail in PBS pH 7.4) and centrifuged at 12,000 g for 15 min at 4°C. Protein concentrations were estimated by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA).
T3375 7865-7877 Sentence denotes Western Blot
T3374 7860-7864 Sentence denotes 2.6.
T1237 4237-4307 Sentence denotes Cells passaged for 2-3 generations were used in the following studies.
T1236 4076-4236 Sentence denotes After shaking, the resulting cultures were mainly astrocytes, which were determined by immunoreactivity for GFAP (sc-166481, Santa Cruz Biotechnology, CA, USA).
T1235 3951-4075 Sentence denotes Then the flasks or dishes were shaken at 150 rpm for 4 h to deplete the microglia and less adherent cells from the cultures.
T1234 3893-3950 Sentence denotes Astrocytes expanded for about 7 days to reach confluence.
T1233 3830-3892 Sentence denotes And the complete culture medium was half-changed twice a week.
T1232 3778-3829 Sentence denotes Then the cells were cultivated at 37°C with 5% CO2.
T1231 3638-3777 Sentence denotes After incubation for 1 h, the supernatant was transferred to a new flask or dish (Costar, USA) for depleting the residual epithelial cells.
T1230 3437-3637 Sentence denotes After digestion, the tissue was rinsed twice in DMEM with 10% FBS, followed by a mechanical dissociation in DMEM with 20% FBS and 5 units/mL penicillin, 5 μg/mL streptomycin (complete culture medium).
T1229 3302-3436 Sentence denotes The tissue was roughly chopped with a scalpel blade, incubated in 0.5% trypsin for 10 minutes at 37°C, and agitated every few minutes.
T1228 3185-3301 Sentence denotes Following decapitation, the cortices were dissected out, and the meninges and associated blood vessels were removed.
T1227 3056-3184 Sentence denotes Primary astrocytes were obtained from postnatal 2-day-old Nrf2 wide type (WT) and knockout (KO) mice (6 mice for each genotype).
T1226 2939-3055 Sentence denotes Nrf2 knockout ICR mice were kindly provided by Dr. Thomas W. Kensler (Johns Hopkins University, Baltimore, MD, USA).
T1225 2884-2938 Sentence denotes Primary Culture and Identification of Mouse Astrocytes
T1224 2879-2883 Sentence denotes 2.1.
T630 2681-2851 Sentence denotes Therefore, here we analysed the influence of Nrf2 on the expression of proinflammatory cytokines in primary cultured astrocytes from transgenic mice after scratch injury.
T629 2587-2680 Sentence denotes Little is known about the relationship between Nrf2 and inflammation in astrocytes after TBI.
T628 2524-2586 Sentence denotes In fact, all the aforementioned studies are performed in vivo.
T627 2389-2523 Sentence denotes It is well documented that astrocytes play a critical role in maintaining normal brain physiology and responding to injury or disease.
T626 2251-2388 Sentence denotes On the contrary, such inflammation can be extenuated through elevating the level of Nrf2 by its inducers, such as sulforaphane (SFN) [6].
T625 2138-2250 Sentence denotes It was also showed that TBI could induce more aggravated damage in Nrf2 knockout mice than in wildtype mice [5].
T624 2018-2137 Sentence denotes Several studies have demonstrated that Nrf2 regulates the inflammation in the brain after traumatic brain injury (TBI).
T623 1894-2017 Sentence denotes Nuclear factor erythroid 2-related factor2 (Nrf2) is a transcription factor that regulates many kinds of antioxidant genes.
T622 1786-1893 Sentence denotes And the subsequent inflammation leads to secondary damage not only in brain but also in other organs [2–4].
T621 1441-1785 Sentence denotes The secondary injury mechanism involves the initiation of an acute inflammatory response, including breakdown of blood-brain barrier (BBB), brain edema, infiltration of peripheral blood cells, and activation of resident immunocompetent cells, as well as the release of numerous immune mediators such as interleukins and chemotactic factors [1].
T620 1307-1440 Sentence denotes Brain damage following traumatic injury is a result of direct (primary injury) and indirect (secondary or delayed injury) mechanisms.
T1 0-119 Sentence denotes The Absence of Nrf2 Enhances NF-κB-Dependent Inflammation following Scratch Injury in Mouse Primary Cultured Astrocytes
T2 121-129 Sentence denotes Abstract
T3 130-341 Sentence denotes It has been proved that Nrf2 depletion enhances inflammatory process through activation of NF-κB in the brain after TBI, but little is known about the relationship between Nrf2 and NF-κB in astrocytes after TBI.
T4 342-557 Sentence denotes Hence, we used primary cultured astrocytes from either Nrf2 wildtype or knockout mice to study the influence of Nrf2 on the activation of NF-κB and expression of proinflammatory cytokines in a model of TBI in vitro.
T5 558-640 Sentence denotes Primary cultured astrocytes were scratched to mimic the traumatic injury in vitro.
T6 641-702 Sentence denotes Then the DNA-binding activity of NF-κB was evaluated by EMSA.
T7 703-783 Sentence denotes The mRNA and protein levels of TNF-α, IL-1β, IL-6, and MMP9 were also evaluated.
T8 784-848 Sentence denotes Gelatin zymography was performed to detect the activity of MMP9.
T9 849-970 Sentence denotes The activity of NF-κB and expression of proinflammatory cytokines mentioned above were upregulated at 24 h after scratch.
T10 971-1026 Sentence denotes The expression and activity of MMP9 were also elevated.
T11 1027-1118 Sentence denotes And such tendency was much more prominent in Nrf2 KO astrocytes than that in WT astrocytes.
T12 1119-1289 Sentence denotes These results suggest that the absence of Nrf2 may induce more aggressive inflammation through activation of NF-κB and downstream proinflammatory cytokines in astrocytes.
T13 1291-1293 Sentence denotes 1.
T14 1294-1306 Sentence denotes Introduction
T15 1307-1440 Sentence denotes Brain damage following traumatic injury is a result of direct (primary injury) and indirect (secondary or delayed injury) mechanisms.
T16 1441-1785 Sentence denotes The secondary injury mechanism involves the initiation of an acute inflammatory response, including breakdown of blood-brain barrier (BBB), brain edema, infiltration of peripheral blood cells, and activation of resident immunocompetent cells, as well as the release of numerous immune mediators such as interleukins and chemotactic factors [1].
T17 1786-1893 Sentence denotes And the subsequent inflammation leads to secondary damage not only in brain but also in other organs [2–4].
T18 1894-2017 Sentence denotes Nuclear factor erythroid 2-related factor2 (Nrf2) is a transcription factor that regulates many kinds of antioxidant genes.
T19 2018-2137 Sentence denotes Several studies have demonstrated that Nrf2 regulates the inflammation in the brain after traumatic brain injury (TBI).
T20 2138-2250 Sentence denotes It was also showed that TBI could induce more aggravated damage in Nrf2 knockout mice than in wildtype mice [5].
T21 2251-2388 Sentence denotes On the contrary, such inflammation can be extenuated through elevating the level of Nrf2 by its inducers, such as sulforaphane (SFN) [6].
T22 2389-2523 Sentence denotes It is well documented that astrocytes play a critical role in maintaining normal brain physiology and responding to injury or disease.
T23 2524-2586 Sentence denotes In fact, all the aforementioned studies are performed in vivo.
T24 2587-2680 Sentence denotes Little is known about the relationship between Nrf2 and inflammation in astrocytes after TBI.
T25 2681-2851 Sentence denotes Therefore, here we analysed the influence of Nrf2 on the expression of proinflammatory cytokines in primary cultured astrocytes from transgenic mice after scratch injury.
T26 2853-2855 Sentence denotes 2.
T27 2856-2877 Sentence denotes Methods and Materials
T28 2879-2883 Sentence denotes 2.1.
T29 2884-2938 Sentence denotes Primary Culture and Identification of Mouse Astrocytes
T30 2939-2999 Sentence denotes Nrf2 knockout ICR mice were kindly provided by Dr. Thomas W.
T31 3000-3055 Sentence denotes Kensler (Johns Hopkins University, Baltimore, MD, USA).
T32 3056-3184 Sentence denotes Primary astrocytes were obtained from postnatal 2-day-old Nrf2 wide type (WT) and knockout (KO) mice (6 mice for each genotype).
T33 3185-3301 Sentence denotes Following decapitation, the cortices were dissected out, and the meninges and associated blood vessels were removed.
T34 3302-3436 Sentence denotes The tissue was roughly chopped with a scalpel blade, incubated in 0.5% trypsin for 10 minutes at 37°C, and agitated every few minutes.
T35 3437-3637 Sentence denotes After digestion, the tissue was rinsed twice in DMEM with 10% FBS, followed by a mechanical dissociation in DMEM with 20% FBS and 5 units/mL penicillin, 5 μg/mL streptomycin (complete culture medium).
T36 3638-3777 Sentence denotes After incubation for 1 h, the supernatant was transferred to a new flask or dish (Costar, USA) for depleting the residual epithelial cells.
T37 3778-3829 Sentence denotes Then the cells were cultivated at 37°C with 5% CO2.
T38 3830-3892 Sentence denotes And the complete culture medium was half-changed twice a week.
T39 3893-3950 Sentence denotes Astrocytes expanded for about 7 days to reach confluence.
T40 3951-4075 Sentence denotes Then the flasks or dishes were shaken at 150 rpm for 4 h to deplete the microglia and less adherent cells from the cultures.
T41 4076-4236 Sentence denotes After shaking, the resulting cultures were mainly astrocytes, which were determined by immunoreactivity for GFAP (sc-166481, Santa Cruz Biotechnology, CA, USA).
T42 4237-4307 Sentence denotes Cells passaged for 2-3 generations were used in the following studies.
T43 4309-4313 Sentence denotes 2.2.
T44 4314-4365 Sentence denotes In Vitro Model of TBI Established by Scratch Injury
T45 4366-4433 Sentence denotes Astrocyte scratch injury was performed as in a previous report [7].
T46 4434-4499 Sentence denotes Astrocytes were planted in 6-well plates and grown to confluence.
T47 4500-4616 Sentence denotes The cell monolayer was scratched with a sterile 26G syringe needle, resulting in the formation of a 0.5 mm wide gap.
T48 4617-4748 Sentence denotes Immediately after scratch, cells were washed twice with sterile PBS, cultured with complete culture medium, and named as TBI group.
T49 4749-4839 Sentence denotes Cells, which did not received scratch, were used as blank control and named as sham group.
T50 4841-4845 Sentence denotes 2.3.
T51 4846-4883 Sentence denotes Cell Death Measurement by CCK-8 Assay
T52 4884-4975 Sentence denotes 1 × 103/well cells were seeded to 96-well culture plates and cultivated for 24 h to adhere.
T53 4976-5021 Sentence denotes Then the cell was scrated as mentioned above.
T54 5022-5170 Sentence denotes Astrocyte cell death was assessed by cell counting kit-8 (CCK-8) (Dojindo, Japan) assay 24 h after scratch according to the manufacturer's protocol.
T55 5171-5240 Sentence denotes Briefly, 10 μL CCK-8 was added into every well and incubated for 1 h.
T56 5241-5346 Sentence denotes Then OD value was read at 450 nm using a Bio-Rad ELISA microplate reader (Bio-Rad Laboratories, CA, USA).
T57 5347-5395 Sentence denotes All measurements were performed in sextuplicate.
T58 5396-5454 Sentence denotes Results were expressed as mean of OD value at 450 nm ± SD.
T59 5456-5460 Sentence denotes 2.4.
T60 5461-5504 Sentence denotes Electrophoretic Mobility Shift Assay (EMSA)
T61 5505-5591 Sentence denotes Monolayers of astrocytes were washed with PBS and harvested by scraping into cold PBS.
T62 5592-5784 Sentence denotes The cell pellet obtained by centrifugation was resuspended in buffer containing 10 mM HEPES (pH 7.9), 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 1.0 mM DTT, and 0.5 mM phenylmethylsulfonyl fluoride.
T63 5785-5886 Sentence denotes Then 10% Nonidet P-40 was added and vortexed briefly, and the nuclei were pelleted by centrifugation.
T64 5887-6060 Sentence denotes The nuclear proteins were extracted with buffer containing 20 mM HEPES (pH 7.9), 0.4 mM NaCl, 1.0 mM EDTA, 1.0 mM EGTA, 1.0 mM DTT, and 1.0 mM phenylmethylsulfonyl fluoride.
T65 6061-6206 Sentence denotes Insoluble material was removed by centrifugation at 14000 rpm, and the supernatant containing the nuclear proteins was stored at –80°C until use.
T66 6207-6364 Sentence denotes Protein concentration was determined using a bicinchoninic acid assay kit with bovine serum albumin as the standard (Pierce Biochemicals, Rockford, IL, USA).
T67 6365-6441 Sentence denotes EMSA was performed using gel shift assay system (Promega, Madison, WI, USA).
T68 6442-6599 Sentence denotes Consensus oligonucleotide probe (5′-AGTTGAGGGGACTTTCCCAGGG-3′) was end-labeled by T4-polynucleotide kinase using [γ-32P]-ATP (Free Biotech., Beijing, China).
T69 6600-6806 Sentence denotes Nuclear protein (20 μg) was preincubated in 20 μL binding buffer containing 10 mM Tris-HCl (PH 7.5), 1 mM MgCl2, 0.5 mM NaCl, 4% glycerol, 0.5 mM EDTA, 0.5 mM DTT, and 2 μg poly dI-dC for 20 minutes on ice.
T70 6807-6919 Sentence denotes After addition of the 1 μL 32P-labled oligonucleotide probe, the incubation was continued for 20 minutes on ice.
T71 6920-7085 Sentence denotes The DNA-protein complexes were separated by electrophoresis on 4% nondenaturing polyacrylamide gel in 0.5 × TBE buffer (tris-borate-EDTA) at 390 V for 1 hour at 4°C.
T72 7086-7184 Sentence denotes After electrophoresis, the gel was dried and exposed to X-ray film (Fuji Hyperfilm, Tokyo, Japan).
T73 7185-7257 Sentence denotes Levels of NF-κB DNA binding activity were quantified by software ImageJ.
T74 7259-7263 Sentence denotes 2.5.
T75 7264-7270 Sentence denotes RT-PCR
T76 7271-7483 Sentence denotes Total RNA was isolated with Trizol (Invitrogen, CA, USA), and single-stranded cDNA was synthesized from 2 μg of total RNA with BU-Script RT-Kit (Biunique, Jiangsu, China) according to the manufacturer's protocol.
T77 7484-7513 Sentence denotes The cDNA was stored in −20°C.
T78 7514-7638 Sentence denotes Reverse transcription was conducted with GoTaq Green Master Mix (Promega, WI, USA) according to the manufacturer's protocol.
T79 7640-7685 Sentence denotes Table 1 shows the primers and PCR parameters.
T80 7686-7744 Sentence denotes PCR products were detected by agarose gel electrophoresis.
T81 7745-7803 Sentence denotes The intensity of the bands was analyzed by ImageJ program.
T82 7804-7858 Sentence denotes The level of β-actin was used as an internal standard.
T83 7860-7864 Sentence denotes 2.6.
T84 7865-7877 Sentence denotes Western Blot
T85 7878-8157 Sentence denotes To obtain total protein lysates, cells were homogenized in RIPA buffer (1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA, 1 mM Na3VO4, 20 mM NaF, 0.5 mM DTT, 1 mM PMSF, and protease inhibitor cocktail in PBS pH 7.4) and centrifuged at 12,000 g for 15 min at 4°C.
T86 8158-8254 Sentence denotes Protein concentrations were estimated by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA).
T87 8255-8496 Sentence denotes Fifty micrograms of the resulting cytosolic protein extracts were heat-denatured in Laemmli sample loading buffer, separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis, and electroblotted onto a nitrocellulose membrane.
T88 8497-8802 Sentence denotes For immunoblotting, membranes were blocked with 5% nonfat dry milk in saline buffer overnight at 4°C, and the following antibodies were used: anti-β-actin (sc-130657, Santa Cruz Biotechnology, CA, USA, 43 kDa) and anti-matrix metallopeptidase 9 (MMP9) (sc-6841, Santa Cruz Biotechnology, CA, USA, 92 kDa).
T89 8803-8926 Sentence denotes Each primary antibody was diluted appropriately in blocking buffer and then added to the blots for 1 h at room temperature.
T90 8927-9084 Sentence denotes The blots were washed three times in the washing buffer and covered with the horseradish peroxidase-linked secondary antibody at a 1 : 2000 dilution for 1 h.
T91 9085-9259 Sentence denotes Blots were incubated with enhanced chemiluminescence (ECL) detection system (Amersham Biosciences, Bucks, UK) and exposed to radiographic film (Fuji Hyperfilm, Tokyo, Japan).
T92 9260-9314 Sentence denotes ImageJ was used to analyze the intensity of the blots.
T93 9315-9366 Sentence denotes The level of β-actin was used as internal standard.
T94 9368-9372 Sentence denotes 2.7.
T95 9373-9414 Sentence denotes Enzyme-Linked Immunosorbent Assay (ELISA)
T96 9415-9472 Sentence denotes Cells of four groups were homogenized as mentioned above.
T97 9473-9595 Sentence denotes The supernatant was collected, and total protein was determined by Coomassie Plus Protein Assay Reagent (Pierce, IL, USA).
T98 9596-9824 Sentence denotes Levels of tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6) protein were quantified using ELISA kits specific for mouse according to the manufacturer's instructions (Bender MedSystems Inc.
T99 9825-9834 Sentence denotes CA, USA).
T100 9835-9928 Sentence denotes Briefly, prepared the standard and created the standard dilution for building standard curve.
T101 9929-9993 Sentence denotes Then samples and biotinconjugate were added to microwell strips.
T102 9994-10143 Sentence denotes After incubated for 2 h at room temperature, the microwell strips were washed 3 times with wash buffer, and streptavidin-HRP were added to all wells.
T103 10144-10239 Sentence denotes After incubated for 1 h, microwell strips were washed 3 times followed by adding TMB substrate.
T104 10240-10305 Sentence denotes After incubated for about 10–30 min, the stop solution was added.
T105 10306-10422 Sentence denotes The colour intensity was measured at 450 nm using a Bio-Rad ELISA microplate reader (Bio-Rad Laboratories, CA, USA).
T106 10423-10539 Sentence denotes The concentration of protein was determined according to the standard curve and expressed as pg/mg of total protein.
T107 10541-10545 Sentence denotes 2.8.
T108 10546-10565 Sentence denotes Gelatine Zymography
T109 10566-10711 Sentence denotes Cells of four groups were homogenized in lysis buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 5 mM CaCl2, 0.2 mM NaN3, and 0.01% Triton.
T110 10712-10782 Sentence denotes Soluble extracts were separated by centrifugation and stored at –20°C.
T111 10783-10895 Sentence denotes Gelatin zymography was performed according to the manufacturer's instructions (Genmed Scientifics Inc, MA, USA).
T112 10896-10972 Sentence denotes Briefly, 40 μg cytosolic protein extracts were separated by electrophoresis.
T113 10973-11138 Sentence denotes Then the proteins were renatured by incubation in 2.5% Triton X-100 and then incubated in substrate buffer for 40 h at 37°C to enable the MMP9 to cleave the gelatin.
T114 11139-11242 Sentence denotes After rinsing in water, each gel was stained with Coomassie blue for 1 h and destained in 50% methanol.
T115 11243-11348 Sentence denotes Proteolytic activities were showed by clear bands in blue gel which indicates the lysis of the substrate.
T116 11349-11434 Sentence denotes Quantification of MMP9 band density was performed with image analysis program ImageJ.
T117 11436-11440 Sentence denotes 2.9.
T118 11441-11461 Sentence denotes Statistical Analysis
T119 11462-11551 Sentence denotes Data were expressed as mean ± SD and evaluated by ANOVA and LSD multiple comparison test.
T120 11552-11601 Sentence denotes P values <0.05 were considered to be significant.
T121 11602-11658 Sentence denotes All analyses were performed by using SPSS 18.0 software.
T122 11660-11662 Sentence denotes 3.
T123 11663-11670 Sentence denotes Results
T124 11672-11676 Sentence denotes 3.1.
T125 11677-11755 Sentence denotes Depletion of Nrf2 Aggravated the Cell Death in Astrocytes after Scratch Injury
T126 11756-11835 Sentence denotes Cell death was detected by microscope and CCK-8 analysis at 24 h after scratch.
T127 11836-11984 Sentence denotes The detachment from culture plate, cell lost, and cytoplasmic process distortion were mostly observed in both sides of scratch line in group KO TBI.
T128 11985-12172 Sentence denotes CCK-8 assay also showed lower OD value of group KO TBI (0.38 ± 0.064) than that of group WT TBI (0.98 ± 0.098) (P < 0.01), which also suggested more cell death in group KO TBI (Figure 1).
T129 12173-12230 Sentence denotes There was no difference between two sham-operated groups.
T130 12232-12236 Sentence denotes 3.2.
T131 12237-12328 Sentence denotes Disruption of Nrf2 Enhanced Upregulation of NF-κB DNA-Binding Activity after Scratch Injury
T132 12329-12397 Sentence denotes It has been reported that NF-κB is activated in brain after TBI [2].
T133 12398-12524 Sentence denotes Here, we studied the DNA-binding activity of NF-κB of astrocytes from Nrf2 WT or KO mice by EMSA at 24 h after scratch injury.
T134 12525-12735 Sentence denotes Scratch injury induced activation of NF-κB in astrocytes of both genotypes, while higher NF-κB activity was observed in group KO TBI than in group WT TBI (2.67 ± 0.173 versus 2.28 ± 0.072, P < 0.01) (Figure 2).
T135 12737-12741 Sentence denotes 3.3.
T136 12742-12843 Sentence denotes Expression of Proinflammatory Cytokines Was Elevated in Nrf2 Knockout Astrocytes after Scratch Injury
T137 12844-12951 Sentence denotes As we all know, TNF-α, IL-1β, and IL-6 are regulated by NF-κB and reflect the endogenous activity of NF-κB.
T138 12952-13052 Sentence denotes It has been revealed that these proinflammatory cytokines are increased after TBI in animals [2, 8].
T139 13053-13204 Sentence denotes And our previous study has proved that higher levels of such proinflammatory cytokines were observed in the brain of Nrf2-deficient mice after TBI [5].
T140 13205-13311 Sentence denotes But it is still ambiguous about the relationship between these cytokines and Nrf2 in astrocytes after TBI.
T141 13312-13437 Sentence denotes Here, we tested the levels of TNF-α, IL-1β, and IL-6 in cultured astrocytes at 24 h after scratch injury by RT-PCR and ELISA.
T142 13438-13613 Sentence denotes It was shown that the mRNA levels of TNF-α, IL-1β, and IL-6 were increased after scratch in both WT and KO astrocytes when compared with their sham counterparts, respectively.
T143 13614-13854 Sentence denotes Moreover, the mRNA levels of these cytokines in group KO TBI were much higher than those in group WT TBI (1.66 ± 0.085 versus 1.39 ± 0.110 for TNF-α, 1.34 ± 0.064 versus 0.73 ± 0.088 for IL-1β, and 1.28 ± 0.102 versus 0.99 ± 0.073 for IL-6.
T144 13855-13885 Sentence denotes P < 0.01) (Figures 3(a)–3(d)).
T145 13886-14052 Sentence denotes The results of ELISA revealed that the protein level of TNF-α was upregulated after scratch injury in WT and KO astrocytes when compared with their sham counterparts.
T146 14053-14172 Sentence denotes And it was also higher in group KO TBI than in group WT TBI (3.67 ± 0.156 versus 2.31 ± 0.087, P < 0.01) (Figure 4(a)).
T147 14173-14238 Sentence denotes Similar tendency was observed in protein level of IL-6 and IL-1β.
T148 14239-14349 Sentence denotes For IL-6, it was 36.07 ± 0.786 for group KO TBI while 25.76 ± 0.536 for group WT TBI (P < 0.01) (Figure 4(b)).
T149 14350-14463 Sentence denotes For IL-1β, it was 190.75 ± 6.339 for group KO TBI while 154.50 ± 5.348 for group WT TBI (P < 0.01) (Figure 4(c)).
T150 14465-14469 Sentence denotes 3.4.
T151 14470-14572 Sentence denotes Expression and Activity of MMP9 Were Greatly Enhanced in Nrf2 Knockout Astrocytes after Scratch Injury
T152 14573-14653 Sentence denotes MMP9 is an important gelatinase to induce or aggravate the inflammation process.
T153 14654-14827 Sentence denotes In the present study, we elevated the mRNA, protein levels, and activity of MMP9 in astrocytes at 24 h after scratch injury by RT-PCR, western blot, and gelatine zymography.
T154 14828-14946 Sentence denotes Expression of MMP9 was elevated after scratch injury in WT and KO astrocytes as compared with their sham counterparts.
T155 14947-15087 Sentence denotes The mRNA level of MMP9 was higher in group KO TBI than in group WT TBI (1.36 ± 0.090 versus 1.01 ± 0.068, P < 0.01) (Figures 3(a) and 3(e)).
T156 15088-15259 Sentence denotes Significant difference was also discovered in MMP9 protein level by western blot, as 1.44 ± 0.076 for group KO TBI and 1.13 ± 0.048 for group WT TBI (P < 0.01) (Figure 5).
T157 15260-15415 Sentence denotes Gelatine zymography revealed higher MMP9 activity in group KO TBI than that in group WT TBI too (101.76 ± 6.343 versus 76.32 ± 3.388, P < 0.01) (Figure 6).
T158 15417-15419 Sentence denotes 4.
T159 15420-15430 Sentence denotes Discussion
T160 15431-15611 Sentence denotes The present study demonstrated that scratch injury induced the upregulation of NF-κB DNA binding activity and overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes.
T161 15612-15825 Sentence denotes Also we revealed that scratch injury induced higher activity of NF-κB and enhanced expression of proinflammatory cytokines in Nrf2 knockout cultured astrocytes than those in wildtype astrocytes for the first time.
T162 15826-15930 Sentence denotes It has been demonstrated that NF-κB is activated in brain and spinal cord after traumatic injury [2, 9].
T163 15931-16149 Sentence denotes As a transcript factor, NF-κB binds with DNA once it was activated and induces transcription of MMP9 and a battery of proinflammatory cytokines, including TNF-α, IL-1β, and IL-6, in brain tissues after TBI [3, 10, 11].
T164 16150-16338 Sentence denotes Our results also revealed that scratch injury induced elevation of NF-κB DNA binding activity, overexpression of MMP9, and proinflammatory mediators mentioned above in cultured astrocytes.
T165 16339-16439 Sentence denotes It has been demonstrated that there is an autoregulatory loop among these proinflammatory mediators.
T166 16440-16667 Sentence denotes For example, TNF-α and IL-1β are potent stimulators for MMP9 in astrocytes [12, 13], and IL-6 induces overexpression of MMP9 in human colon carcinoma cells [14], and TNF-α and IL-1β also can induce activation of NF-κB [15, 16].
T167 16668-16788 Sentence denotes This autoregulatory loop extremely aggravates the damaging effect of inflammation and induces secondary injury to brain.
T168 16789-16891 Sentence denotes It is a good choice for targeting on an upstream factor to prevent such autoregulatory loop after TBI.
T169 16892-17069 Sentence denotes Nrf2-ARE pathway has been proved to be the key regulator in reducing oxidative stress, inflammatory damage, and accumulation of toxic metabolites, which are all involved in TBI.
T170 17070-17156 Sentence denotes Our previous study has proved the augmentation of Nrf2 in brain tissue after TBI [17].
T171 17157-17373 Sentence denotes Enhanced level of Nrf2 activates transcription of a group of antioxidant genes, such as heme oxygenase-1 (HO-1) and NAD (P)H: quinone oxidoreductase-1 (NQO1), which would subsequently reduce the damage in brain [18].
T172 17374-17635 Sentence denotes Postinjury administration of SFN, an inducer of Nrf2, significantly improves spatial memory of rat and decreases the immunoreactivity for 4-Hydroxynonenal (4-HNE), a marker of lipid peroxidation, in the cortex and the CA3 subfield of hippocampus after TBI [19].
T173 17636-17706 Sentence denotes On the other hand, Nrf2-deficient mice appear more susceptible to TBI.
T174 17707-17804 Sentence denotes Depletion of Nrf2 induces higher expression of proinflammatory mediators in brain after TBI [20].
T175 17805-18095 Sentence denotes Here, our results revealed that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch injury was more aggravated in cultured Nrf2 knockout astrocytes than in wildtype astrocyetes for the first time, and overexpression of these proinflammatory mediators led to more astrocytes deaths.
T176 18096-18283 Sentence denotes Data obtained from animal studies suggest the possibility that antioxidant effect of Nrf2 may be achieved by suppression of proinflammatory pathways which are mediated by NF-κB signaling.
T177 18284-18452 Sentence denotes Administration of SFN is found to be able to inhibit IKK/IκB phosphorylation and p65 NF-κB subunit nuclear translocation, consequently alleviating NF-κB signaling [21].
T178 18453-18594 Sentence denotes And NF-κB activation induced by lipopolysaccharide (LPS) could be attenuated by diverse Nrf2 activators, such as SFN and curcumin (CUR) [22].
T179 18595-18777 Sentence denotes Furthermore, our previous studies indicate that depletion of Nrf2 induces augmentation of NF-κB activity and inflammatory response in lung, brain, and intestine after TBI [4, 5, 23].
T180 18778-18890 Sentence denotes Results from this study further confirmed such relationship existed in cultured astrocytes after scratch injury.
T181 18891-19047 Sentence denotes Enhanced activation of NF-κB is also discovered in lung, macrophages, and mouse embryonic fibroblasts of Nrf2-deficient mice after experimental sepsis [24].
T182 19048-19131 Sentence denotes Interestingly, it is reported that NF-κB can inhibit Nrf2 at transcriptional level.
T183 19132-19302 Sentence denotes NF-κB p65 subunit repressed the Nrf2-ARE pathway at transcriptional level by competitive interaction with the CH1-KIX domain of CBP or local histone hypoacetylation [25].
T184 19303-19483 Sentence denotes All these findings indicate the potential complicate crosstalk between NF-κB and Nrf2, which may be regulated by the upstream mitogen-activated protein kinase (MAPKs) pathway [26].
T185 19484-19575 Sentence denotes It has been confirmed that Nrf2 is mainly detected in nucleus of astrocytes after TBI [18].
T186 19576-19793 Sentence denotes In view of the fact that Nrf2 is a transcription factor which should take function mainly in nucleus, it can be reasoned that astrocytes may be one kind of respondent cells in activation of Nrf2-ARE pathway after TBI.
T187 19794-19935 Sentence denotes Previous study identified the expression of TNF-α, IL-1β, and IL-6 in cultured astrocytes after treatment with LPS or oxyhemoglobin [27, 28].
T188 19936-20089 Sentence denotes Another study revealed that after middle cerebral artery occlusion, MMP9-positive astrocytes were observed in brain tissues by immunohistochemistry [29].
T189 20090-20179 Sentence denotes Those results indicate the role of astrocytes in inflammatory process after brain injury.
T190 20180-20304 Sentence denotes But till now, there is no study focused on the relationship among astrocytes, Nrf2, and proinflammatory mediators after TBI.
T191 20305-20457 Sentence denotes Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9 in Nrf2 knockout astrocytes after scratch injury for the first time.
T192 20458-20639 Sentence denotes In conclusion, depletion of Nrf2 induced the activation of NF-κB and the expression of TNF-α, IL-1β, IL-6, and MMP9 resulting in more cell deaths in astrocytes after scratch injury.
T193 20640-20739 Sentence denotes These results suggest that Nrf2 may be an important target for anti-inflammatory therapy after TBI.
T194 20741-20878 Sentence denotes Figure 1 Loss of Nrf2 enhanced cell death after scratch. (a) Cultured astrocytes scratched or not were observed under microscope (×100).
T195 20879-21000 Sentence denotes There was more cell death in group KO TBI. (b) CCK-8 analysis revealed lower OD value in scratched astrocytes (P < 0.01).
T196 21001-21156 Sentence denotes Group KO TBI showed significant lower OD value than group WT TBI (P < 0.01). (*P < 0.01, compared with sham counterpart; # P < 0.01, compared with WT TBI).
T197 21157-21404 Sentence denotes Figure 2 EMSA analysis for DNA-binding activity of NF-κB. (a) Representative film shows DNA-binding activity of NF-κB in Nrf2 WT or KO astrocytes 24 h after scratch or not. (b) Quantification of NF-κB DNA-binding activity was performed by ImageJ.
T198 21405-21636 Sentence denotes NF-κB DNA-binding activity in astrocytes increased after scratch (P < 0.01) and was prominently greater in group KO TBI than in group WT TBI (P < 0.01). (*P < 0.01, compared with sham counterpart; # P < 0.01, compared with WT TBI).
T199 21637-22072 Sentence denotes Figure 3 RT-PCR analysis of mRNA level of proinflammatory cytokines and MMP9. (a) RT-PCR showed that mRNA level of TNF-α, IL-1β, IL-6, and MMP9 was elevated in astrocytes after scratch and was significantly higher in group KO TBI than in group WT TBI. (b–e) Quantitative analysis of RT-PCR results showed that relative mRNA level of proinflammatory cytokines mentioned above and MMP9 was higher in astrocytes after scratch (P < 0.01).
T200 22073-22232 Sentence denotes Such elevation was more severe in group KO TBI than in group WT TBI (P < 0.01).  (*P < 0.01, compared with sham counterpart; # P < 0.01, compared with WT TBI).
T201 22233-22304 Sentence denotes Figure 4 ELISA analysis of protein level of proinflammatory cytokines.
T202 22305-22444 Sentence denotes ELISA results revealed protein level of these cytokines were elevated in astrocytes after scratch as compared with sham control (P < 0.01).
T203 22445-22645 Sentence denotes And they were much higher in Nrf2 KO astrocytes than in their WT counterparts (P < 0.01). (a) TNF-α. (b) IL-6. (c) IL-1β  (*P < 0.01, compared with sham counterpart; # P < 0.01, compared with WT TBI).
T204 22646-23002 Sentence denotes Figure 5 Western blot analysis of MMP9 protein level. (a) Western blot showed that protein level of MMP9 was elevated in astrocytes after scratch and was significantly higher in group KO TBI than in group WT TBI. (b) Quantitative analysis of western blot result showed that relative protein level of MMP9 was higher in astrocytes after scratch (P < 0.01).
T205 23003-23161 Sentence denotes Such elevation was much severe in group KO TBI than in group WT TBI (P < 0.01). (*P < 0.01, compared with sham counterpart; # P < 0.01, compared with WT TBI).
T206 23162-23409 Sentence denotes Figure 6 Gelatin zymography of MMP9 activity. (a) Gelatin zymography analysis showed higher MMP9 activity in group KO TBI than in group WT TBI. (b) Quantitative analysis showed that MMP9 activity was higher in astrocytes after scratch (P < 0.01).
T207 23410-23568 Sentence denotes Such elevation was much severe in group KO TBI than in group WT TBI (P < 0.01). (*P < 0.01, compared with sham counterpart; # P < 0.01, compared with WT TBI).
T208 23569-23617 Sentence denotes Table 1 Primers and parameters used in RT-PCR.

ICD10

Id Subject Object Predicate Lexical cue
T904 2844-2850 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T903 2505-2511 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T902 2124-2130 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T901 1455-1461 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T900 1421-1427 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T899 1378-1384 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T898 1340-1346 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T8277 20632-20638 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T8276 20431-20437 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T8275 20172-20178 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T8274 18883-18889 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T8273 17898-17904 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T8272 16772-16778 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T8271 16189-16195 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T8270 15916-15922 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T8269 15642-15648 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T8268 15475-15481 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T6699 14874-14880 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T6698 14771-14777 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T6697 14566-14572 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes Injury
T5829 13978-13984 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T5828 13410-13416 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T5827 12837-12843 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes Injury
T255 76-82 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes Injury
T256 624-630 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T8288 19977-20002 http://purl.bioontology.org/ontology/ICD10/I66.9 denotes cerebral artery occlusion
T5212 12533-12539 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T5211 12517-12523 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T5210 12322-12328 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes Injury
T4964 11749-11755 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes Injury
T1918 4384-4390 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes injury
T1917 4359-4365 http://purl.bioontology.org/ontology/ICD10/T14.9 denotes Injury

simple1

Id Subject Object Predicate Lexical cue
T8356 20569-20573 Protein denotes MMP9
T8355 20559-20563 Protein denotes IL-6
T8354 20552-20557 Protein denotes IL-1β
T8353 20384-20388 Protein denotes MMP9
T8352 20374-20378 Protein denotes IL-6
T8351 20367-20372 Protein denotes IL-1β
T8350 20004-20008 Protein denotes MMP9
T8349 19856-19860 Protein denotes IL-6
T8348 19845-19850 Protein denotes IL-1β
T8347 19138-19141 Protein denotes p65
T8346 18365-18368 Protein denotes p65
T8345 17879-17883 Protein denotes MMP9
T8344 17869-17873 Protein denotes IL-6
T8343 17862-17867 Protein denotes IL-1β
T8342 17263-17267 Protein denotes HO-1
T8341 17245-17261 Protein denotes heme oxygenase-1
T8340 16616-16621 Protein denotes IL-1β
T8339 16560-16564 Protein denotes MMP9
T8338 16529-16533 Protein denotes IL-6
T8337 16496-16500 Protein denotes MMP9
T8336 16463-16468 Protein denotes IL-1β
T8335 16263-16267 Protein denotes MMP9
T8334 16104-16108 Protein denotes IL-6
T8333 16093-16098 Protein denotes IL-1β
T8332 16027-16031 Protein denotes MMP9
T8331 15583-15587 Protein denotes MMP9
T8330 15573-15577 Protein denotes IL-6
T8329 15566-15571 Protein denotes IL-1β
T4551 11367-11371 Protein denotes MMP9
T4550 11111-11115 Protein denotes MMP9
T3225 7817-7824 Protein denotes β-actin
T264 1002-1006 Protein denotes MMP9
T263 843-847 Protein denotes MMP9
T4288 9690-9694 Protein denotes IL-6
T4287 9675-9688 Protein denotes interleukin-6
T6707 15296-15300 Protein denotes MMP9
T6706 15134-15138 Protein denotes MMP9
T6705 14965-14969 Protein denotes MMP9
T6704 14842-14846 Protein denotes MMP9
T6703 14730-14734 Protein denotes MMP9
T6702 14573-14577 Protein denotes MMP9
T6701 14497-14501 Protein denotes MMP9
T5848 14354-14359 Protein denotes IL-1β
T5847 14243-14247 Protein denotes IL-6
T5846 14232-14237 Protein denotes IL-1β
T5845 14223-14227 Protein denotes IL-6
T5844 13849-13853 Protein denotes IL-6
T5843 13801-13806 Protein denotes IL-1β
T5842 13493-13497 Protein denotes IL-6
T5841 13482-13487 Protein denotes IL-1β
T5840 13360-13364 Protein denotes IL-6
T5839 13349-13354 Protein denotes IL-1β
T5838 12878-12882 Protein denotes IL-6
T5837 12867-12872 Protein denotes IL-1β
T4286 9663-9668 Protein denotes IL-1β
T4285 9643-9661 Protein denotes interleukin-1 beta
T3686 8743-8747 Protein denotes MMP9
T260 741-746 Protein denotes IL-1β
T261 748-752 Protein denotes IL-6
T262 758-762 Protein denotes MMP9

BioNLP16_DUT

Id Subject Object Predicate Lexical cue
T9937 20531-20541 Gene_expression denotes expression
T9936 20491-20498 Positive_regulation denotes induced
T9935 20346-20356 Gene_expression denotes expression
T9934 20334-20345 Positive_regulation denotes upregulated
T9933 20009-20017 Gene_expression denotes positive
T9932 19824-19834 Gene_expression denotes expression
T9931 19150-19159 Negative_regulation denotes repressed
T9930 18329-18336 Negative_regulation denotes inhibit
T9929 18391-18404 Localization denotes translocation
T9928 17914-17924 Positive_regulation denotes aggravated
T9927 17837-17851 Positive_regulation denotes overexpression
T9926 17190-17203 Transcription denotes transcription
T9925 17180-17189 Positive_regulation denotes activates
T9924 16542-16556 Positive_regulation denotes overexpression
T9923 16534-16541 Positive_regulation denotes induces
T9922 16245-16259 Positive_regulation denotes overexpression
T9921 16245-16259 Gene_expression denotes overexpression
T9920 16002-16009 Positive_regulation denotes induces
T9919 16010-16023 Transcription denotes transcription
T9918 15541-15555 Gene_expression denotes overexpression
T9917 15482-15489 Positive_regulation denotes induced
T9916 15541-15555 Positive_regulation denotes overexpression
T9915 20569-20573 Protein denotes MMP9
T9914 20559-20563 Protein denotes IL-6
T9913 20552-20557 Protein denotes IL-1β
T9912 20384-20388 Protein denotes MMP9
T9911 20374-20378 Protein denotes IL-6
T9910 20367-20372 Protein denotes IL-1β
T9909 20004-20008 Protein denotes MMP9
T9908 19856-19860 Protein denotes IL-6
T9907 19845-19850 Protein denotes IL-1β
T9906 19138-19141 Protein denotes p65
T9905 18365-18368 Protein denotes p65
T9904 17879-17883 Protein denotes MMP9
T9903 17869-17873 Protein denotes IL-6
T9902 17862-17867 Protein denotes IL-1β
T9901 17263-17267 Protein denotes HO-1
T9900 17245-17261 Protein denotes heme oxygenase-1
T9899 16496-16500 Protein denotes MMP9
T9898 16463-16468 Protein denotes IL-1β
T9897 16616-16621 Protein denotes IL-1β
T9896 16560-16564 Protein denotes MMP9
T9895 16529-16533 Protein denotes IL-6
T9894 16263-16267 Protein denotes MMP9
T9893 16104-16108 Protein denotes IL-6
T9892 16093-16098 Protein denotes IL-1β
T9891 16027-16031 Protein denotes MMP9
T9890 15583-15587 Protein denotes MMP9
T9889 15573-15577 Protein denotes IL-6
T9888 15566-15571 Protein denotes IL-1β
T6985 14956-14961 Transcription denotes level
T6984 14851-14859 Positive_regulation denotes elevated
T6983 14828-14838 Gene_expression denotes Expression
T6982 14679-14687 Positive_regulation denotes elevated
T6981 14470-14480 Gene_expression denotes Expression
T6980 14515-14523 Positive_regulation denotes Enhanced
T6979 15296-15300 Protein denotes MMP9
T6978 15134-15138 Protein denotes MMP9
T6977 14965-14969 Protein denotes MMP9
T6976 14842-14846 Protein denotes MMP9
T6975 14730-14734 Protein denotes MMP9
T6974 14573-14577 Protein denotes MMP9
T6973 14497-14501 Protein denotes MMP9
T6367 14214-14219 Positive_regulation denotes level
T6366 13465-13471 Transcription denotes levels
T6365 13503-13512 Positive_regulation denotes increased
T6364 12887-12896 Regulation denotes regulated
T6363 14354-14359 Protein denotes IL-1β
T6362 14243-14247 Protein denotes IL-6
T6361 14223-14227 Protein denotes IL-6
T6360 14232-14237 Protein denotes IL-1β
T6359 13849-13853 Protein denotes IL-6
T6358 13801-13806 Protein denotes IL-1β
T6357 13493-13497 Protein denotes IL-6
T6356 13482-13487 Protein denotes IL-1β
T6355 13360-13364 Protein denotes IL-6
T6354 13349-13354 Protein denotes IL-1β
T6353 12878-12882 Protein denotes IL-6
T6352 12867-12872 Protein denotes IL-1β
T4732 11367-11371 Protein denotes MMP9
T4731 11111-11115 Protein denotes MMP9
T4337 9690-9694 Protein denotes IL-6
T4336 9675-9688 Protein denotes interleukin-6
T4335 9663-9668 Protein denotes IL-1β
T4334 9643-9661 Protein denotes interleukin-1 beta
T3997 8743-8747 Protein denotes MMP9
T3353 7817-7824 Protein denotes β-actin
T555 741-746 Protein denotes IL-1β
T556 748-752 Protein denotes IL-6
T557 758-762 Protein denotes MMP9
T558 843-847 Protein denotes MMP9
T559 1002-1006 Protein denotes MMP9
T560 1017-1025 Positive_regulation denotes elevated
T561 975-985 Gene_expression denotes expression
R5802 T6973 T6981 themeOf MMP9,Expression
R5803 T6975 T6982 themeOf MMP9,elevated
R5804 T6976 T6983 themeOf MMP9,Expression
R5805 T6977 T6985 themeOf MMP9,level
R5806 T6981 T6980 themeOf Expression,Enhanced
R5807 T6983 T6984 themeOf Expression,elevated
R8145 T9902 T9927 themeOf IL-1β,overexpression
R8146 T9888 T9918 themeOf IL-1β,overexpression
R8147 T9903 T9927 themeOf IL-6,overexpression
R8148 T9904 T9927 themeOf MMP9,overexpression
R8149 T9905 T9929 themeOf p65,translocation
R8150 T9889 T9918 themeOf IL-6,overexpression
R8151 T9906 T9931 themeOf p65,repressed
R8152 T9890 T9918 themeOf MMP9,overexpression
R8153 T9907 T9932 themeOf IL-1β,expression
R8154 T9891 T9919 themeOf MMP9,transcription
R8155 T9908 T9932 themeOf IL-6,expression
R8156 T9894 T9921 themeOf MMP9,overexpression
R8157 T9909 T9933 themeOf MMP9,positive
R8158 T9896 T9924 themeOf MMP9,overexpression
R8159 T9910 T9935 themeOf IL-1β,expression
R8160 T9900 T9926 themeOf heme oxygenase-1,transcription
R8161 T9911 T9935 themeOf IL-6,expression
R8162 T9901 T9926 themeOf HO-1,transcription
R8163 T9912 T9935 themeOf MMP9,expression
R8164 T9913 T9937 themeOf IL-1β,expression
R8165 T9914 T9937 themeOf IL-6,expression
R8166 T9915 T9937 themeOf MMP9,expression
R8167 T9916 T9917 themeOf overexpression,induced
R8168 T9916 T9917 themeOf overexpression,induced
R8169 T9916 T9917 themeOf overexpression,induced
R8170 T9918 T9917 themeOf overexpression,induced
R8171 T9918 T9917 themeOf overexpression,induced
R8172 T9918 T9917 themeOf overexpression,induced
R8173 T9918 T9916 themeOf overexpression,overexpression
R8174 T9918 T9916 themeOf overexpression,overexpression
R8175 T9918 T9916 themeOf overexpression,overexpression
R8176 T9919 T9920 themeOf transcription,induces
R8177 T9921 T9922 themeOf overexpression,overexpression
R8181 T9924 T9923 themeOf overexpression,induces
R8183 T9926 T9925 themeOf transcription,activates
R8185 T9926 T9925 themeOf transcription,activates
R8186 T9927 T9928 themeOf overexpression,aggravated
R8190 T9927 T9928 themeOf overexpression,aggravated
R8191 T9927 T9928 themeOf overexpression,aggravated
R8193 T9929 T9930 themeOf translocation,inhibit
R8194 T9935 T9934 themeOf expression,upregulated
R8199 T9935 T9934 themeOf expression,upregulated
R8201 T9935 T9934 themeOf expression,upregulated
R8202 T9937 T9936 themeOf expression,induced
R8205 T9937 T9936 themeOf expression,induced
R8206 T9937 T9936 themeOf expression,induced
R446 T559 T561 themeOf MMP9,expression
R447 T561 T560 themeOf expression,elevated
R5354 T6352 T6364 themeOf IL-1β,regulated
R5355 T6353 T6364 themeOf IL-6,regulated
R5356 T6356 T6365 themeOf IL-1β,increased
R5357 T6356 T6366 themeOf IL-1β,levels
R5358 T6357 T6365 themeOf IL-6,increased
R5359 T6360 T6367 themeOf IL-1β,level
R5360 T6361 T6367 themeOf IL-6,level

BioNLP16_Messiy

Id Subject Object Predicate Lexical cue
T8494 20491-20498 Positive_regulation denotes induced
T8493 20531-20541 Gene_expression denotes expression
T8492 20473-20482 Negative_regulation denotes depletion
T8491 20334-20345 Positive_regulation denotes upregulated
T8490 20346-20356 Gene_expression denotes expression
T8489 20009-20017 Gene_expression denotes positive
T8488 19824-19834 Gene_expression denotes expression
T8487 19221-19232 Binding denotes interaction
T8486 19150-19159 Negative_regulation denotes repressed
T8485 18329-18336 Negative_regulation denotes inhibit
T8484 18391-18404 Localization denotes translocation
T8483 17914-17924 Positive_regulation denotes aggravated
T8482 17837-17851 Positive_regulation denotes overexpression
T8481 17180-17189 Positive_regulation denotes activates
T8480 17190-17203 Transcription denotes transcription
T8479 16534-16541 Positive_regulation denotes induces
T8478 16542-16556 Positive_regulation denotes overexpression
T8477 16245-16259 Positive_regulation denotes overexpression
T8476 16196-16203 Positive_regulation denotes induced
T8475 16002-16009 Positive_regulation denotes induces
T8474 16010-16023 Transcription denotes transcription
T8473 15541-15555 Positive_regulation denotes overexpression
T8472 15482-15489 Positive_regulation denotes induced
T8471 20569-20573 Protein denotes MMP9
T8470 20559-20563 Protein denotes IL-6
T8469 20552-20557 Protein denotes IL-1β
T8468 20384-20388 Protein denotes MMP9
T8467 20374-20378 Protein denotes IL-6
T8466 20367-20372 Protein denotes IL-1β
T8465 20004-20008 Protein denotes MMP9
T8464 19856-19860 Protein denotes IL-6
T8463 19845-19850 Protein denotes IL-1β
T8462 19138-19141 Protein denotes p65
T8454 16463-16468 Protein denotes IL-1β
T8453 16616-16621 Protein denotes IL-1β
T8452 16560-16564 Protein denotes MMP9
T8451 16529-16533 Protein denotes IL-6
T8450 16263-16267 Protein denotes MMP9
T8449 16104-16108 Protein denotes IL-6
T8448 16093-16098 Protein denotes IL-1β
T8447 16027-16031 Protein denotes MMP9
T8446 15583-15587 Protein denotes MMP9
T8445 15573-15577 Protein denotes IL-6
T8444 15566-15571 Protein denotes IL-1β
T6736 15289-15295 Positive_regulation denotes higher
T6735 14974-14980 Positive_regulation denotes higher
T6734 14956-14961 Transcription denotes level
T6733 14828-14838 Gene_expression denotes Expression
T6732 14851-14859 Positive_regulation denotes elevated
T6731 14679-14687 Positive_regulation denotes elevated
T6730 14515-14523 Positive_regulation denotes Enhanced
T6729 14470-14480 Gene_expression denotes Expression
T6728 15296-15300 Protein denotes MMP9
T6727 15134-15138 Protein denotes MMP9
T6726 14965-14969 Protein denotes MMP9
T6725 14842-14846 Protein denotes MMP9
T6724 14730-14734 Protein denotes MMP9
T6723 14573-14577 Protein denotes MMP9
T6722 14497-14501 Protein denotes MMP9
T5898 13503-13512 Positive_regulation denotes increased
T5897 12887-12896 Regulation denotes regulated
T5896 14354-14359 Protein denotes IL-1β
T5895 14243-14247 Protein denotes IL-6
T5894 14223-14227 Protein denotes IL-6
T5893 14232-14237 Protein denotes IL-1β
T5892 13849-13853 Protein denotes IL-6
T5891 13801-13806 Protein denotes IL-1β
T5890 13493-13497 Protein denotes IL-6
T5889 13482-13487 Protein denotes IL-1β
T5888 13360-13364 Protein denotes IL-6
T5887 13349-13354 Protein denotes IL-1β
T5886 12878-12882 Protein denotes IL-6
T5885 12867-12872 Protein denotes IL-1β
T4299 9690-9694 Protein denotes IL-6
T4298 9675-9688 Protein denotes interleukin-6
T4297 9663-9668 Protein denotes IL-1β
T4296 9643-9661 Protein denotes interleukin-1 beta
T4556 11119-11125 Protein_catabolism denotes cleave
T4555 11367-11371 Protein denotes MMP9
T4554 11111-11115 Protein denotes MMP9
T3690 8743-8747 Protein denotes MMP9
T3228 7817-7824 Protein denotes β-actin
T281 975-985 Gene_expression denotes expression
T268 741-746 Protein denotes IL-1β
T269 748-752 Protein denotes IL-6
T270 758-762 Protein denotes MMP9
T271 843-847 Protein denotes MMP9
T272 1002-1006 Protein denotes MMP9
T273 1017-1025 Positive_regulation denotes elevated
T8461 18365-18368 Protein denotes p65
T8460 17879-17883 Protein denotes MMP9
T8459 17869-17873 Protein denotes IL-6
T8458 17862-17867 Protein denotes IL-1β
T8457 17263-17267 Protein denotes HO-1
T8456 17245-17261 Protein denotes heme oxygenase-1
T8455 16496-16500 Protein denotes MMP9
R3872 T4554 T4556 themeOf MMP9,cleave
R4943 T5885 T5897 themeOf IL-1β,regulated
R4944 T5886 T5897 themeOf IL-6,regulated
R4945 T5889 T5898 themeOf IL-1β,increased
R4946 T5890 T5898 themeOf IL-6,increased
R5580 T6722 T6729 themeOf MMP9,Expression
R5581 T6724 T6731 themeOf MMP9,elevated
R5582 T6725 T6733 themeOf MMP9,Expression
R5583 T6725 T6732 themeOf MMP9,elevated
R5584 T6726 T6734 themeOf MMP9,level
R5585 T6726 T6735 themeOf MMP9,higher
R5586 T6728 T6736 themeOf MMP9,higher
R5587 T6729 T6730 themeOf Expression,Enhanced
R5588 T6733 T6732 themeOf Expression,elevated
R6868 T8444 T8473 themeOf IL-1β,overexpression
R6869 T8445 T8473 themeOf IL-6,overexpression
R6870 T8446 T8472 themeOf MMP9,induced
R6871 T8446 T8473 themeOf MMP9,overexpression
R6872 T8447 T8474 themeOf MMP9,transcription
R6873 T8450 T8477 themeOf MMP9,overexpression
R6874 T8450 T8476 themeOf MMP9,induced
R6875 T8452 T8478 themeOf MMP9,overexpression
R6876 T8456 T8480 themeOf heme oxygenase-1,transcription
R6877 T8457 T8480 themeOf HO-1,transcription
R6878 T8458 T8483 themeOf IL-1β,aggravated
R6879 T8458 T8482 themeOf IL-1β,overexpression
R6880 T8459 T8482 themeOf IL-6,overexpression
R6881 T8460 T8483 themeOf MMP9,aggravated
R6882 T8460 T8482 themeOf MMP9,overexpression
R6883 T8461 T8484 themeOf p65,translocation
R6884 T8462 T8487 themeOf p65,interaction
R6885 T8462 T8486 themeOf p65,repressed
R6886 T8463 T8488 themeOf IL-1β,expression
R6887 T8464 T8488 themeOf IL-6,expression
R6888 T8465 T8489 themeOf MMP9,positive
R6889 T8466 T8490 themeOf IL-1β,expression
R6890 T8467 T8490 themeOf IL-6,expression
R6891 T8468 T8490 themeOf MMP9,expression
R6892 T8469 T8493 themeOf IL-1β,expression
R6893 T8470 T8493 themeOf IL-6,expression
R6894 T8471 T8493 themeOf MMP9,expression
R6895 T8473 T8472 themeOf overexpression,induced
R6896 T8473 T8472 themeOf overexpression,induced
R6897 T8473 T8472 themeOf overexpression,induced
R6898 T8474 T8475 themeOf transcription,induces
R6899 T8478 T8479 themeOf overexpression,induces
R6900 T8480 T8481 themeOf transcription,activates
R6901 T8480 T8481 themeOf transcription,activates
R6902 T8482 T8483 themeOf overexpression,aggravated
R6903 T8482 T8483 themeOf overexpression,aggravated
R6904 T8482 T8483 themeOf overexpression,aggravated
R6905 T8484 T8485 themeOf translocation,inhibit
R6906 T8490 T8491 themeOf expression,upregulated
R6907 T8490 T8491 themeOf expression,upregulated
R6908 T8490 T8491 themeOf expression,upregulated
R6909 T8493 T8494 themeOf expression,induced
R6910 T8493 T8494 themeOf expression,induced
R6911 T8493 T8494 themeOf expression,induced
R6912 T8494 T8492 themeOf induced,depletion
R6913 T8494 T8492 themeOf induced,depletion
R6914 T8494 T8492 themeOf induced,depletion
R206 T281 T273 themeOf expression,elevated
R443 T272 T281 themeOf MMP9,expression

DLUT931

Id Subject Object Predicate Lexical cue
T8443 20503-20513 Positive_regulation denotes activation
T8442 20531-20541 Gene_expression denotes expression
T8441 20334-20345 Positive_regulation denotes upregulated
T8440 20346-20356 Gene_expression denotes expression
T8439 20009-20017 Gene_expression denotes positive
T8438 19824-19834 Gene_expression denotes expression
T8437 19150-19159 Negative_regulation denotes repressed
T8436 19221-19232 Binding denotes interaction
T8435 18329-18336 Negative_regulation denotes inhibit
T8434 18391-18404 Localization denotes translocation
T8433 17914-17924 Positive_regulation denotes aggravated
T8432 17837-17851 Positive_regulation denotes overexpression
T8431 17180-17189 Positive_regulation denotes activates
T8430 17190-17203 Transcription denotes transcription
T8429 16534-16541 Positive_regulation denotes induces
T8428 16542-16556 Positive_regulation denotes overexpression
T8427 16196-16203 Positive_regulation denotes induced
T8426 16245-16259 Positive_regulation denotes overexpression
T8425 16002-16009 Positive_regulation denotes induces
T8424 16010-16023 Transcription denotes transcription
T8423 15494-15506 Positive_regulation denotes upregulation
T8422 15482-15489 Positive_regulation denotes induced
T8421 15541-15555 Gene_expression denotes overexpression
T8420 20569-20573 Protein denotes MMP9
T8419 20559-20563 Protein denotes IL-6
T8418 20552-20557 Protein denotes IL-1β
T8417 20384-20388 Protein denotes MMP9
T8416 20374-20378 Protein denotes IL-6
T8415 20367-20372 Protein denotes IL-1β
T8414 20004-20008 Protein denotes MMP9
T8413 19856-19860 Protein denotes IL-6
T8412 19845-19850 Protein denotes IL-1β
T8411 19138-19141 Protein denotes p65
T8410 18365-18368 Protein denotes p65
T8409 17879-17883 Protein denotes MMP9
T8408 17869-17873 Protein denotes IL-6
T8407 17862-17867 Protein denotes IL-1β
T8406 17263-17267 Protein denotes HO-1
T8405 17245-17261 Protein denotes heme oxygenase-1
T8404 16496-16500 Protein denotes MMP9
T8403 16463-16468 Protein denotes IL-1β
T8402 16616-16621 Protein denotes IL-1β
T8401 16560-16564 Protein denotes MMP9
T8400 16529-16533 Protein denotes IL-6
T8399 16263-16267 Protein denotes MMP9
T8398 16104-16108 Protein denotes IL-6
T8397 16093-16098 Protein denotes IL-1β
T8396 16027-16031 Protein denotes MMP9
T8395 15583-15587 Protein denotes MMP9
T8394 15573-15577 Protein denotes IL-6
T8393 15566-15571 Protein denotes IL-1β
T6721 14974-14980 Positive_regulation denotes higher
T6720 14956-14961 Transcription denotes level
T6719 14851-14859 Positive_regulation denotes elevated
T6718 14828-14838 Gene_expression denotes Expression
T6717 14679-14687 Positive_regulation denotes elevated
T6716 14515-14523 Positive_regulation denotes Enhanced
T6715 14470-14480 Gene_expression denotes Expression
T6714 15296-15300 Protein denotes MMP9
T6713 15134-15138 Protein denotes MMP9
T6712 14965-14969 Protein denotes MMP9
T6711 14842-14846 Protein denotes MMP9
T6710 14730-14734 Protein denotes MMP9
T6709 14573-14577 Protein denotes MMP9
T6708 14497-14501 Protein denotes MMP9
T5884 13503-13512 Positive_regulation denotes increased
T5883 13465-13471 Transcription denotes levels
T5882 12887-12896 Regulation denotes regulated
T5881 14354-14359 Protein denotes IL-1β
T5880 14243-14247 Protein denotes IL-6
T5879 14223-14227 Protein denotes IL-6
T5878 14232-14237 Protein denotes IL-1β
T5877 13849-13853 Protein denotes IL-6
T5876 13801-13806 Protein denotes IL-1β
T5875 13493-13497 Protein denotes IL-6
T5874 13482-13487 Protein denotes IL-1β
T5873 13360-13364 Protein denotes IL-6
T5872 13349-13354 Protein denotes IL-1β
T5871 12878-12882 Protein denotes IL-6
T5870 12867-12872 Protein denotes IL-1β
T4295 9690-9694 Protein denotes IL-6
T4294 9675-9688 Protein denotes interleukin-6
T4293 9663-9668 Protein denotes IL-1β
T4292 9643-9661 Protein denotes interleukin-1 beta
T4553 11367-11371 Protein denotes MMP9
T4552 11111-11115 Protein denotes MMP9
T3689 8743-8747 Protein denotes MMP9
T3227 7817-7824 Protein denotes β-actin
T274 741-746 Protein denotes IL-1β
T275 748-752 Protein denotes IL-6
T276 758-762 Protein denotes MMP9
T277 843-847 Protein denotes MMP9
T278 1002-1006 Protein denotes MMP9
T279 975-985 Gene_expression denotes expression
T280 1017-1025 Positive_regulation denotes elevated
R6832 T8411 T8436 themeOf p65,interaction
R6833 T8412 T8438 themeOf IL-1β,expression
R6835 T8414 T8439 themeOf MMP9,positive
R6836 T8415 T8440 themeOf IL-1β,expression
R6839 T8418 T8442 themeOf IL-1β,expression
R6842 T8421 T8422 themeOf overexpression,induced
R6843 T8421 T8423 themeOf overexpression,upregulation
R6844 T8421 T8422 themeOf overexpression,induced
R6845 T8421 T8422 themeOf overexpression,induced
R6846 T8421 T8423 themeOf overexpression,upregulation
R6847 T8421 T8423 themeOf overexpression,upregulation
R6848 T8423 T8422 themeOf upregulation,induced
R6849 T8423 T8422 themeOf upregulation,induced
R6850 T8423 T8422 themeOf upregulation,induced
R6851 T8424 T8425 themeOf transcription,induces
R6852 T8424 T8425 themeOf transcription,induces
R6853 T8426 T8427 themeOf overexpression,induced
R6854 T8428 T8429 themeOf overexpression,induces
R6855 T8430 T8431 themeOf transcription,activates
R6856 T8430 T8431 themeOf transcription,activates
R6857 T8432 T8433 themeOf overexpression,aggravated
R6860 T8434 T8435 themeOf translocation,inhibit
R6861 T8436 T8437 themeOf interaction,repressed
R6862 T8440 T8441 themeOf expression,upregulated
R6865 T8442 T8443 themeOf expression,activation
R444 T278 T279 themeOf MMP9,expression
R445 T279 T280 themeOf expression,elevated
R4937 T5870 T5882 themeOf IL-1β,regulated
R4938 T5871 T5882 themeOf IL-6,regulated
R4939 T5874 T5883 themeOf IL-1β,levels
R4940 T5875 T5883 themeOf IL-6,levels
R4941 T5883 T5884 themeOf levels,increased
R4942 T5883 T5884 themeOf levels,increased
R5573 T6708 T6715 themeOf MMP9,Expression
R5574 T6710 T6717 themeOf MMP9,elevated
R5575 T6711 T6718 themeOf MMP9,Expression
R5576 T6712 T6720 themeOf MMP9,level
R5577 T6715 T6716 themeOf Expression,Enhanced
R5578 T6718 T6719 themeOf Expression,elevated
R5579 T6720 T6721 themeOf level,higher
R6818 T8393 T8421 themeOf IL-1β,overexpression
R6819 T8394 T8421 themeOf IL-6,overexpression
R6820 T8395 T8421 themeOf MMP9,overexpression
R6821 T8396 T8424 themeOf MMP9,transcription
R6822 T8398 T8424 themeOf IL-6,transcription
R6823 T8399 T8426 themeOf MMP9,overexpression
R6824 T8400 T8429 causeOf IL-6,induces
R6825 T8401 T8428 themeOf MMP9,overexpression
R6826 T8405 T8430 themeOf heme oxygenase-1,transcription
R6827 T8406 T8430 themeOf HO-1,transcription
R6828 T8407 T8432 themeOf IL-1β,overexpression
R6829 T8408 T8432 themeOf IL-6,overexpression
R6831 T8410 T8434 themeOf p65,translocation
R6830 T8409 T8432 themeOf MMP9,overexpression
R6834 T8413 T8438 themeOf IL-6,expression
R6837 T8416 T8440 themeOf IL-6,expression
R6838 T8417 T8440 themeOf MMP9,expression
R6858 T8432 T8433 themeOf overexpression,aggravated
R6859 T8432 T8433 themeOf overexpression,aggravated
R6863 T8440 T8441 themeOf expression,upregulated
R6840 T8419 T8442 themeOf IL-6,expression
R6841 T8420 T8442 themeOf MMP9,expression
R6864 T8440 T8441 themeOf expression,upregulated
R6866 T8442 T8443 themeOf expression,activation
R6867 T8442 T8443 themeOf expression,activation

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue Speculation
T10101 19820-19883 Gene_expression denotes the expression of TNF-α, IL-1β, and IL-6 in cultured astrocytes
T10100 19820-19883 Gene_expression denotes the expression of TNF-α, IL-1β, and IL-6 in cultured astrocytes
T10099 19820-19883 Gene_expression denotes the expression of TNF-α, IL-1β, and IL-6 in cultured astrocytes
T10098 16542-16564 Positive_regulation denotes overexpression of MMP9
T10097 16542-16564 Gene_expression denotes overexpression of MMP9
T10096 15634-15734 Positive_regulation denotes scratch injury induced higher activity of NF-κB and enhanced expression of proinflammatory cytokines
T10095 20330-20388 Positive_regulation denotes the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9
T10094 20330-20388 Positive_regulation denotes the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9
T10093 20330-20388 Positive_regulation denotes the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9
T10092 20330-20388 Positive_regulation denotes the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9
T10091 15490-15536 Positive_regulation denotes the upregulation of NF-κB DNA binding activity
T10090 20527-20631 Gene_expression denotes the expression of TNF-α, IL-1β, IL-6, and MMP9 resulting in more cell deaths in astrocytes after scratch
T10089 20527-20631 Gene_expression denotes the expression of TNF-α, IL-1β, IL-6, and MMP9 resulting in more cell deaths in astrocytes after scratch
T10088 20527-20631 Gene_expression denotes the expression of TNF-α, IL-1β, IL-6, and MMP9 resulting in more cell deaths in astrocytes after scratch
T10087 20527-20631 Gene_expression denotes the expression of TNF-α, IL-1β, IL-6, and MMP9 resulting in more cell deaths in astrocytes after scratch
T10086 19078-19130 Negative_regulation denotes that NF-κB can inhibit Nrf2 at transcriptional level true
T10085 18264-18282 Positive_regulation denotes by NF-κB signaling
T10084 17832-17897 Gene_expression denotes that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch
T10083 17832-17897 Positive_regulation denotes that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch
T10082 17832-17897 Gene_expression denotes that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch
T10081 17832-17897 Positive_regulation denotes that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch
T10080 17832-17897 Gene_expression denotes that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch
T10079 17832-17897 Gene_expression denotes that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch
T10078 17832-17897 Positive_regulation denotes that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch
T10077 17832-17897 Positive_regulation denotes that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch
T10076 18431-18446 Positive_regulation denotes NF-κB signaling
T10075 15851-15922 Positive_regulation denotes that NF-κB is activated in brain and spinal cord after traumatic injury
T10074 15695-15734 Gene_expression denotes expression of proinflammatory cytokines
T10073 15541-15610 Gene_expression denotes overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes
T10072 15541-15610 Gene_expression denotes overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes
T10071 15541-15610 Positive_regulation denotes overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes
T10039 17717-17724 Protein denotes of Nrf2
T10038 18457-18532 Protein denotes NF-κB activation induced by lipopolysaccharide (LPS) could be attenuated by
T10037 16260-16268 Protein denotes of MMP9,
T10036 15786-15824 Entity denotes wildtype astrocytes for the first time
T10035 16318-16337 Entity denotes cultured astrocytes
T10034 15955-15960 Protein denotes NF-κB
T10033 20122-20135 Entity denotes of astrocytes
T10032 20406-20416 Entity denotes astrocytes
T10031 17862-17867 Protein denotes IL-1β
T10030 17117-17140 Protein denotes of Nrf2 in brain tissue
T10029 19596-19605 Protein denotes that Nrf2
T10028 20384-20388 Protein denotes MMP9
T10027 16086-16091 Protein denotes TNF-α
T10026 20374-20378 Protein denotes IL-6
T10025 20667-20671 Protein denotes Nrf2
T10024 17879-17897 Protein denotes MMP9 after scratch
T10023 19011-19046 Protein denotes mice after experimental sepsis [24]
T10022 20569-20573 Protein denotes MMP9
T10021 17263-17267 Protein denotes HO-1
T10020 17017-17037 Entity denotes of toxic metabolites
T10019 19538-19559 Entity denotes nucleus of astrocytes
T10018 19838-19843 Protein denotes TNF-α
T10017 20552-20557 Protein denotes IL-1β
T10016 17245-17315 Entity denotes heme oxygenase-1 (HO-1) and NAD (P)H: quinone oxidoreductase-1 (NQO1),
T10015 16649-16657 Protein denotes of NF-κB
T10014 15706-15734 Protein denotes of proinflammatory cytokines
T10013 18685-18690 Protein denotes NF-κB
T10012 18505-18508 Entity denotes LPS
T10011 19101-19105 Protein denotes Nrf2
T10010 19549-19559 Entity denotes astrocytes
T10009 15573-15577 Protein denotes IL-6
T10008 15566-15571 Protein denotes IL-1β
T10007 17869-17873 Protein denotes IL-6
T10006 18178-18185 Protein denotes of Nrf2
T10005 17403-17407 Protein denotes SFN,
T10004 19609-19676 Protein denotes a transcription factor which should take function mainly in nucleus
T10003 18302-18305 Protein denotes SFN
T10002 15967-15975 Entity denotes with DNA
T10001 18482-18509 Entity denotes by lipopolysaccharide (LPS)
T10000 17655-17674 Protein denotes Nrf2-deficient mice
T9999 19083-19088 Protein denotes NF-κB
T9998 18546-18556 Entity denotes activators
T9997 19856-19860 Protein denotes IL-6
T9996 16217-16268 Protein denotes NF-κB DNA binding activity, overexpression of MMP9,
T9995 17218-17229 Entity denotes antioxidant
T9994 19463-19468 Protein denotes MAPKs
T9993 20004-20008 Protein denotes MMP9
T9992 17592-17595 Protein denotes CA3
T9991 19273-19280 Entity denotes histone
T9990 15591-15610 Entity denotes cultured astrocytes
T9989 20545-20550 Protein denotes TNF-α
T9988 17283-17305 Protein denotes quinone oxidoreductase
T9987 19132-19149 Protein denotes NF-κB p65 subunit
T9986 19845-19850 Protein denotes IL-1β
T9985 18365-18368 Protein denotes p65
T9984 16104-16108 Protein denotes IL-6
T9983 16606-16611 Protein denotes TNF-α
T9982 19384-19389 Protein denotes Nrf2,
T9981 16616-16621 Protein denotes IL-1β
T9980 17942-17961 Entity denotes knockout astrocytes
T9979 16463-16468 Protein denotes IL-1β
T9978 19246-19249 Entity denotes KIX
T9977 18566-18569 Protein denotes SFN
T9976 20258-20262 Protein denotes Nrf2
T9975 19260-19263 Protein denotes CBP
T9974 15559-15564 Protein denotes TNF-α
T9973 19864-19883 Entity denotes cultured astrocytes
T9972 18911-18919 Protein denotes of NF-κB
T9971 18069-18094 Entity denotes to more astrocytes deaths
T9970 20367-20372 Protein denotes IL-1β
T9969 19413-19482 Protein denotes by the upstream mitogen-activated protein kinase (MAPKs) pathway [26]
T9968 17218-17236 Protein denotes antioxidant genes,
T9967 15510-15515 Protein denotes NF-κB
T9966 18849-18868 Entity denotes cultured astrocytes
T9965 15583-15587 Protein denotes MMP9
T9964 20360-20365 Protein denotes TNF-α
T9963 19374-19379 Protein denotes NF-κB
T9962 16529-16533 Protein denotes IL-6
T9961 20607-20617 Entity denotes astrocytes
T9960 16024-16031 Protein denotes of MMP9
T9959 19912-19925 Entity denotes oxyhemoglobin
T9958 18431-18436 Protein denotes NF-κB
T9957 16557-16564 Protein denotes of MMP9
T9956 18267-18272 Protein denotes NF-κB
T9955 17278-17279 Entity denotes P
T9954 18948-18959 Entity denotes macrophages
T9953 16504-16519 Entity denotes astrocytes [12,
T9952 15934-15946 Protein denotes a transcript
T9951 20246-20263 Entity denotes astrocytes, Nrf2,
T9950 20559-20563 Protein denotes IL-6
T9949 19233-19256 Entity denotes with the CH1-KIX domain
T9948 16093-16098 Protein denotes IL-1β
T9947 15851-15861 Protein denotes that NF-κB
T9946 17855-17860 Protein denotes TNF-α
T9945 18365-18404 Protein denotes p65 NF-κB subunit nuclear translocation
T9944 19132-19137 Protein denotes NF-κB
T9943 19743-19792 Entity denotes cells in activation of Nrf2-ARE pathway after TBI
T9942 15673-15681 Protein denotes of NF-κB
T9941 16496-16500 Protein denotes MMP9
T9940 16065-16108 Protein denotes cytokines, including TNF-α, IL-1β, and IL-6
T9939 19702-19712 Entity denotes astrocytes
T9938 20009-20028 Entity denotes positive astrocytes
T10070 15541-15610 Positive_regulation denotes overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes
T10069 15541-15610 Positive_regulation denotes overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes
T10068 15541-15610 Positive_regulation denotes overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes
T10067 15541-15610 Gene_expression denotes overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes
T10066 15541-15610 Gene_expression denotes overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes
T10065 17117-17141 Localization denotes of Nrf2 in brain tissue
T10064 16010-16031 Transcription denotes transcription of MMP9
T10063 20305-20388 Gene_expression denotes Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9
T10062 20305-20388 Gene_expression denotes Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9
T10061 20305-20388 Gene_expression denotes Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9
T10060 20305-20388 Gene_expression denotes Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9
T10059 18891-18992 Positive_regulation denotes Enhanced activation of NF-κB is also discovered in lung, macrophages, and mouse embryonic fibroblasts
T10058 17004-17037 Binding denotes accumulation of toxic metabolites
T10057 18457-18532 Negative_regulation denotes NF-κB activation induced by lipopolysaccharide (LPS) could be attenuated by true
T10056 18891-18919 Positive_regulation denotes Enhanced activation of NF-κB
T10055 16638-16666 Positive_regulation denotes activation of NF-κB [15, 16]
T10054 15955-15997 Binding denotes NF-κB binds with DNA once it was activated
T10053 20499-20522 Protein denotes the activation of NF-κB
T10052 17309-17313 Protein denotes NQO1
T10051 18574-18588 Entity denotes curcumin (CUR)
T10050 15761-15771 Entity denotes astrocytes
T10049 17273-17280 Entity denotes NAD (P)
T10048 18965-18992 Entity denotes mouse embryonic fibroblasts
T10047 19905-19908 Entity denotes LPS
T10046 20703-20738 Entity denotes anti-inflammatory therapy after TBI
T10045 19669-19676 Entity denotes nucleus
T10044 17550-17555 Entity denotes lipid
T10043 19511-19515 Protein denotes Nrf2
T10042 16453-16458 Protein denotes TNF-α
T10041 18159-18170 Entity denotes antioxidant
T10040 17245-17268 Protein denotes heme oxygenase-1 (HO-1)
T7009 14828-14846 Gene_expression denotes Expression of MMP9
T7008 14965-14969 Protein denotes MMP9
T7007 15260-15268 Entity denotes Gelatine
T7006 14951-14955 Protein denotes mRNA
T7005 14891-14904 Entity denotes KO astrocytes
T7004 14990-14992 Protein denotes KO
T7003 14698-14705 Protein denotes protein
T7002 15196-15198 Protein denotes KO
T7001 14891-14893 Protein denotes KO
T7000 14532-14551 Entity denotes Knockout Astrocytes
T6999 14839-14846 Protein denotes of MMP9
T6998 15296-15300 Protein denotes MMP9
T6997 14928-14932 Entity denotes sham
T6996 14738-14748 Entity denotes astrocytes
T6995 14527-14531 Protein denotes Nrf2
T6994 14494-14501 Protein denotes of MMP9
T6993 14807-14815 Entity denotes gelatine
T6992 14573-14577 Protein denotes MMP9
T6991 14727-14734 Protein denotes of MMP9
T6990 15319-15321 Protein denotes KO
T6989 14755-14826 Entity denotes h after scratch injury by RT-PCR, western blot, and gelatine zymography
T6988 14688-14697 Protein denotes the mRNA,
T6987 14781-14787 Protein denotes RT-PCR
T6986 15134-15138 Protein denotes MMP9
T6414 13360-13394 Localization denotes IL-6 in cultured astrocytes at 24 
T6413 13282-13310 Localization denotes Nrf2 in astrocytes after TBI
T6412 12860-12950 Regulation denotes TNF-α, IL-1β, and IL-6 are regulated by NF-κB and reflect the endogenous activity of NF-κB
T6411 12860-12950 Regulation denotes TNF-α, IL-1β, and IL-6 are regulated by NF-κB and reflect the endogenous activity of NF-κB
T6410 12860-12950 Regulation denotes TNF-α, IL-1β, and IL-6 are regulated by NF-κB and reflect the endogenous activity of NF-κB
T6409 13368-13387 Entity denotes cultured astrocytes
T6408 14034-14038 Entity denotes sham
T6407 13282-13310 Protein denotes Nrf2 in astrocytes after TBI
T6406 12803-12822 Entity denotes Knockout Astrocytes
T6405 13542-13555 Entity denotes KO astrocytes
T6404 13995-13997 Protein denotes KO
T6403 13417-13426 Protein denotes by RT-PCR
T6402 14085-14087 Protein denotes KO
T6401 12942-12950 Protein denotes of NF-κB
T6400 13542-13555 Protein denotes KO astrocytes
T6399 13349-13354 Protein denotes IL-1β
T6398 12897-12905 Protein denotes by NF-κB
T6397 12867-12872 Protein denotes IL-1β
T6396 13628-13632 Protein denotes mRNA
T6395 12878-12882 Protein denotes IL-6
T6394 13482-13487 Protein denotes IL-1β
T6393 13995-14008 Entity denotes KO astrocytes
T6392 14280-14282 Protein denotes KO
T6391 13757-13763 Protein denotes TNF-α,
T6390 13942-13947 Protein denotes TNF-α
T6389 12860-12865 Protein denotes TNF-α
T6388 13643-13674 Protein denotes these cytokines in group KO TBI
T6387 13668-13670 Protein denotes KO
T6386 13493-13497 Protein denotes IL-6
T6385 12798-12802 Protein denotes Nrf2
T6384 13394-13426 Entity denotes h after scratch injury by RT-PCR
T6383 14243-14247 Protein denotes IL-6
T6382 13290-13310 Entity denotes astrocytes after TBI
T6381 13575-13585 Entity denotes their sham
T6380 14354-14358 Protein denotes IL-1
T6379 14223-14227 Protein denotes IL-6
T6378 13801-13806 Protein denotes IL-1β
T6377 13360-13394 Protein denotes IL-6 in cultured astrocytes at 24 
T6376 13167-13189 Protein denotes of Nrf2-deficient mice
T6375 13460-13464 Protein denotes mRNA
T6374 14393-14395 Protein denotes KO
T6373 13342-13347 Protein denotes TNF-α
T6372 13262-13277 Protein denotes these cytokines
T6371 13849-13853 Protein denotes IL-6
T6370 13921-13947 Protein denotes the protein level of TNF-α
T6369 14232-14237 Protein denotes IL-1β
T6368 13475-13480 Protein denotes TNF-α
T5356 12237-12328 Positive_regulation denotes Disruption of Nrf2 Enhanced Upregulation of NF-κB DNA-Binding Activity after Scratch Injury
T5355 12237-12255 Negative_regulation denotes Disruption of Nrf2
T5354 12350-12396 Positive_regulation denotes that NF-κB is activated in brain after TBI [2]
T5353 12350-12360 Protein denotes that NF-κB
T5352 12479-12486 Protein denotes KO mice
T5351 12440-12448 Protein denotes of NF-κB
T5350 12651-12653 Protein denotes KO
T5349 12468-12475 Protein denotes Nrf2 WT
T5348 12571-12600 Entity denotes astrocytes of both genotypes,
T5347 12248-12255 Protein denotes of Nrf2
T5346 12498-12516 Entity denotes 24 h after scratch
T5345 12559-12567 Protein denotes of NF-κB
T5344 12287-12298 Entity denotes DNA-Binding
T5343 12278-12307 Protein denotes of NF-κB DNA-Binding Activity
T5342 12452-12486 Entity denotes astrocytes from Nrf2 WT or KO mice
T5341 12614-12619 Protein denotes NF-κB
T5340 12479-12481 Protein denotes KO
T5101 11706-11755 Entity denotes the Cell Death in Astrocytes after Scratch Injury
T5100 11724-11734 Entity denotes Astrocytes
T5099 12148-12171 Protein denotes group KO TBI (Figure 1)
T5098 11819-11834 Entity denotes h after scratch
T5097 11977-11979 Protein denotes KO
T5096 11871-11880 Entity denotes cell lost
T5095 12033-12035 Protein denotes KO
T5094 11798-11803 Protein denotes CCK-8
T5093 11710-11714 Entity denotes Cell
T5092 12209-12222 Entity denotes sham-operated
T5091 11687-11694 Protein denotes of Nrf2
T5090 11985-11990 Protein denotes CCK-8
T4842 11522-11525 Protein denotes LSD
T4841 11485-11494 Entity denotes mean ± SD
T4840 11512-11517 Protein denotes ANOVA
T4752 10546-10554 Entity denotes Gelatine
T4751 11063-11072 Entity denotes substrate
T4750 10905-10937 Entity denotes 40 μg cytosolic protein extracts
T4749 10566-10586 Entity denotes Cells of four groups
T4748 11208-11211 Entity denotes 1 h
T4747 11023-11040 Entity denotes 2.5% Triton X-100
T4746 10712-10728 Entity denotes Soluble extracts
T4745 11107-11115 Protein denotes the MMP9
T4744 10681-10710 Entity denotes 0.2 mM NaN3, and 0.01% Triton
T4743 10698-10710 Entity denotes 0.01% Triton
T4742 10631-10645 Entity denotes 50 mM Tris-HCl
T4741 11296-11347 Entity denotes blue gel which indicates the lysis of the substrate
T4740 10656-10667 Entity denotes 150 mM NaCl
T4739 11163-11171 Entity denotes each gel
T4738 11367-11371 Protein denotes MMP9
T4737 10905-10937 Protein denotes 40 μg cytosolic protein extracts
T4736 10783-10790 Entity denotes Gelatin
T4735 10978-10990 Protein denotes the proteins
T4734 10631-10645 Entity denotes 50 mM Tris-HCl
T4733 11229-11241 Entity denotes 50% methanol
T4361 9696-9703 Protein denotes protein
T4360 10014-10038 Entity denotes 2 h at room temperature,
T4359 9663-9668 Protein denotes IL-1β
T4358 10102-10118 Protein denotes streptavidin-HRP
T4357 9555-9576 Entity denotes Protein Assay Reagent
T4356 9690-9694 Protein denotes IL-6
T4355 9635-9640 Protein denotes TNF-α
T4354 10102-10118 Protein denotes streptavidin-HRP
T4353 9473-9488 Entity denotes The supernatant
T4352 10115-10118 Protein denotes HRP
T4351 10441-10451 Protein denotes of protein
T4350 9675-9688 Protein denotes interleukin-6
T4349 9508-9521 Protein denotes total protein
T4348 10164-10168 Entity denotes 1 h,
T4347 9586-9588 Entity denotes IL
T4346 9603-9641 Protein denotes of tumor necrosis factor-alpha (TNF-α)
T4345 9415-9471 Entity denotes Cells of four groups were homogenized as mentioned above
T4344 9555-9576 Entity denotes Protein Assay Reagent
T4343 10225-10238 Entity denotes TMB substrate
T4342 10277-10294 Entity denotes the stop solution
T4341 9555-9576 Entity denotes Protein Assay Reagent
T4340 9802-9824 Entity denotes Bender MedSystems Inc.
T4339 10525-10538 Protein denotes total protein
T4338 9643-9670 Protein denotes interleukin-1 beta (IL-1β),
T4033 9184-9193 Protein denotes Bucks, UK
T4032 8237-8247 Entity denotes Pierce, IL
T4031 8155-8165 Protein denotes C. Protein
T4030 9139-9142 Protein denotes ECL
T4029 9328-9335 Protein denotes β-actin
T4028 8307-8315 Entity denotes extracts
T4027 8567-8573 Entity denotes saline
T4026 8472-8495 Entity denotes nitrocellulose membrane
T4025 8030-8039 Entity denotes 20 mM NaF
T4024 8603-8627 Entity denotes the following antibodies
T4023 7995-8016 Entity denotes 1 mM EDTA, 1 mM EGTA,
T4022 8053-8062 Entity denotes 1 mM PMSF
T4021 8743-8747 Protein denotes MMP9
T4020 8711-8748 Protein denotes anti-matrix metallopeptidase 9 (MMP9)
T4019 8472-8486 Entity denotes nitrocellulose
T4018 8068-8076 Protein denotes protease
T4017 8299-8306 Protein denotes protein
T4016 8380-8444 Entity denotes by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis
T4015 8808-8824 Protein denotes primary antibody
T4014 7911-7916 Entity denotes cells
T4013 7888-7910 Protein denotes total protein lysates,
T4012 7985-7993 Protein denotes 0.1% SDS
T4011 9034-9052 Protein denotes secondary antibody
T4010 8228-8248 Entity denotes Reagent (Pierce, IL,
T4009 8380-8444 Entity denotes by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis
T4008 7959-7994 Entity denotes 0.5% sodium deoxycholate, 0.1% SDS,
T4007 8633-8663 Protein denotes used: anti-β-actin (sc-130657,
T4006 8517-8526 Entity denotes membranes
T4005 8214-8221 Entity denotes Protein
T4004 8987-9026 Protein denotes covered with the horseradish peroxidase
T4003 8410-8424 Entity denotes polyacrylamide
T4002 8068-8095 Entity denotes protease inhibitor cocktail
T4001 8759-8764 Entity denotes Santa
T4000 8664-8688 Entity denotes Santa Cruz Biotechnology
T3999 8006-8015 Entity denotes 1 mM EGTA
T3998 8041-8051 Entity denotes 0.5 mM DTT
T3095 5887-5957 Negative_regulation denotes The nuclear proteins were extracted with buffer containing 20 mM HEPES
T3094 5731-5742 Entity denotes 1.0 mM DTT,
T3093 5672-5683 Entity denotes 10 mM HEPES
T3092 6252-6270 Entity denotes bicinchoninic acid
T3091 5712-5717 Entity denotes EDTA,
T3090 6345-6363 Entity denotes Rockford, IL, USA)
T3089 6676-6690 Entity denotes 10 mM Tris-HCl
T3088 6773-6777 Entity denotes poly
T3087 6752-6762 Entity denotes 0.5 mM DTT
T3086 6207-6232 Entity denotes Protein concentration was
T3085 6920-6945 Entity denotes The DNA-protein complexes
T3084 6713-6738 Entity denotes 0.5 mM NaCl, 4% glycerol,
T3083 5700-5704 Entity denotes KCl,
T3082 6286-6306 Protein denotes bovine serum albumin
T3081 5843-5853 Entity denotes the nuclei
T3080 5685-5687 Protein denotes pH
T3079 6983-7018 Entity denotes 4% nondenaturing polyacrylamide gel
T3078 6739-6763 Entity denotes 0.5 mM EDTA, 0.5 mM DTT,
T3077 7109-7116 Entity denotes the gel
T3076 5747-5783 Entity denotes 0.5 mM phenylmethylsulfonyl fluoride
T3075 5994-6018 Entity denotes 1.0 mM EGTA, 1.0 mM DTT,
T3074 6524-6548 Entity denotes T4-polynucleotide kinase
T3062 6155-6175 Protein denotes the nuclear proteins
T3061 6324-6343 Entity denotes Pierce Biochemicals
T3060 5821-5829 Entity denotes vortexed
T3059 5887-5907 Protein denotes The nuclear proteins
T3058 6527-6548 Protein denotes polynucleotide kinase
T3057 6250-6280 Entity denotes a bicinchoninic acid assay kit
T3056 6524-6548 Entity denotes T4-polynucleotide kinase
T3055 6562-6582 Entity denotes -ATP (Free Biotech.,
T3054 5790-5806 Entity denotes 10% Nonidet P-40
T3053 6788-6805 Entity denotes 20 minutes on ice
T3052 7195-7200 Protein denotes NF-κB
T3051 7040-7044 Entity denotes tris
T3050 6390-6393 Entity denotes gel
T3049 7000-7014 Entity denotes polyacrylamide
T3048 7040-7056 Entity denotes tris-borate-EDTA
T3047 7045-7051 Entity denotes borate
T3046 6676-6690 Entity denotes 10 mM Tris-HCl
T3045 6901-6918 Entity denotes 20 minutes on ice
T3044 5592-5638 Entity denotes The cell pellet obtained by centrifugation was
T3043 5694-5729 Entity denotes 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA
T3042 5981-5992 Entity denotes 1.0 mM EDTA
T3041 6007-6017 Entity denotes 1.0 mM DTT
T3040 5946-5957 Entity denotes 20 mM HEPES
T3039 5519-5529 Entity denotes astrocytes
T3366 7271-7280 Protein denotes Total RNA
T3365 7686-7689 Protein denotes PCR
T3364 7333-7353 Entity denotes single-stranded cDNA
T3363 7484-7492 Entity denotes The cDNA
T3362 7380-7392 Protein denotes of total RNA
T3361 7299-7318 Entity denotes Trizol (Invitrogen,
T3360 7398-7441 Protein denotes BU-Script RT-Kit (Biunique, Jiangsu, China)
T3359 7375-7392 Protein denotes 2 μg of total RNA
T3358 7264-7270 Protein denotes RT-PCR
T3357 7716-7743 Entity denotes agarose gel electrophoresis
T3356 7716-7723 Entity denotes agarose
T3355 7670-7673 Protein denotes PCR
T3354 7817-7824 Protein denotes β-actin
T3073 6692-6694 Protein denotes PH
T3072 6128-6175 Entity denotes the supernatant containing the nuclear proteins
T3071 6726-6737 Entity denotes 4% glycerol
T3070 6023-6059 Entity denotes 1.0 mM phenylmethylsulfonyl fluoride
T3069 7028-7057 Entity denotes TBE buffer (tris-borate-EDTA)
T3068 5959-5961 Protein denotes pH
T3067 6600-6615 Protein denotes Nuclear protein
T3066 7040-7056 Entity denotes tris-borate-EDTA
T3065 6442-6504 Entity denotes Consensus oligonucleotide probe (5′-AGTTGAGGGGACTTTCCCAGGG-3′)
T3064 6822-6866 Entity denotes of the 1 μL 32P-labled oligonucleotide probe
T3063 5968-5993 Entity denotes 0.4 mM NaCl, 1.0 mM EDTA,
T2307 5186-5191 Entity denotes CCK-8
T2306 5080-5085 Entity denotes CCK-8
T2305 5236-5239 Entity denotes 1 h
T2304 4963-4974 Entity denotes h to adhere
T2303 5022-5031 Entity denotes Astrocyte
T2302 5059-5063 Entity denotes cell
T2301 5110-5128 Entity denotes 24 h after scratch
T2300 4872-4877 Entity denotes CCK-8
T2299 4892-4902 Entity denotes well cells
T2298 4981-4989 Entity denotes the cell
T2297 4846-4850 Entity denotes Cell
T1812 3056-3151 Binding denotes Primary astrocytes were obtained from postnatal 2-day-old Nrf2 wide type (WT) and knockout (KO)
T1811 3893-3903 Entity denotes Astrocytes
T1810 3152-3183 Protein denotes mice (6 mice for each genotype)
T1809 3056-3074 Entity denotes Primary astrocytes
T1808 2919-2938 Entity denotes of Mouse Astrocytes
T1807 3158-3182 Protein denotes 6 mice for each genotype
T1806 3114-3118 Protein denotes Nrf2
T1805 4019-4032 Entity denotes the microglia
T1804 4004-4007 Entity denotes 4 h
T1803 3747-3776 Entity denotes the residual epithelial cells
T1802 3495-3503 Protein denotes 10% FBS,
T1801 2939-2943 Protein denotes Nrf2
T1800 4184-4200 Protein denotes GFAP (sc-166481,
T1799 3590-3636 Entity denotes 5 μg/mL streptomycin (complete culture medium)
T1798 3569-3588 Entity denotes units/mL penicillin
T1797 4201-4226 Protein denotes Santa Cruz Biotechnology,
T1796 4237-4242 Entity denotes Cells
T1795 4037-4056 Entity denotes less adherent cells
T1794 3555-3562 Protein denotes 20% FBS
T1793 4126-4137 Entity denotes astrocytes,
T1792 3664-3679 Entity denotes the supernatant
T1791 3148-3150 Protein denotes KO
T1790 3368-3403 Entity denotes 0.5% trypsin for 10 minutes at 37°C
T1789 3659-3662 Entity denotes 1 h
T1218 2697-2850 Regulation denotes we analysed the influence of Nrf2 on the expression of proinflammatory cytokines in primary cultured astrocytes from transgenic mice after scratch injury
T1217 2731-2850 Gene_expression denotes on the expression of proinflammatory cytokines in primary cultured astrocytes from transgenic mice after scratch injury
T1216 2232-2249 Protein denotes wildtype mice [5]
T1215 1996-2016 Protein denotes of antioxidant genes
T1214 2659-2669 Entity denotes astrocytes
T1213 2205-2209 Protein denotes Nrf2
T1212 2749-2777 Protein denotes of proinflammatory cytokines
T1211 2335-2339 Protein denotes Nrf2
T1210 1938-1942 Protein denotes Nrf2
T1209 2343-2383 Entity denotes its inducers, such as sulforaphane (SFN)
T1208 2057-2061 Protein denotes Nrf2
T1207 1607-1632 Entity denotes of peripheral blood cells
T1206 1999-2010 Entity denotes antioxidant
T1205 2205-2223 Protein denotes Nrf2 knockout mice
T1204 2379-2382 Protein denotes SFN
T1203 2814-2850 Protein denotes transgenic mice after scratch injury
T1202 2634-2638 Protein denotes Nrf2
T1201 2781-2850 Entity denotes primary cultured astrocytes from transgenic mice after scratch injury
T1200 2416-2426 Entity denotes astrocytes
T1199 1744-1756 Protein denotes interleukins
T1198 2723-2730 Protein denotes of Nrf2
T1197 1661-1682 Entity denotes immunocompetent cells
T2031 4673-4723 Regulation denotes sterile PBS, cultured with complete culture medium
T2030 4644-4649 Entity denotes cells
T2029 4500-4508 Entity denotes The cell
T2028 4366-4432 Entity denotes Astrocyte scratch injury was performed as in a previous report [7]
T2027 4434-4444 Entity denotes Astrocytes
T2026 4673-4723 Protein denotes sterile PBS, cultured with complete culture medium
T2025 4749-4769 Entity denotes Cells, which did not
T2024 4828-4832 Entity denotes sham
T2023 4540-4547 Protein denotes sterile
T595 490-547 Gene_expression denotes expression of proinflammatory cytokines in a model of TBI
T594 462-485 Positive_regulation denotes the activation of NF-κB
T593 207-250 Positive_regulation denotes activation of NF-κB in the brain after TBI,
T592 875-924 Gene_expression denotes expression of proinflammatory cytokines mentioned
T591 311-340 Protein denotes NF-κB in astrocytes after TBI
T590 320-330 Entity denotes astrocytes
T589 999-1006 Protein denotes of MMP9
T588 357-384 Entity denotes primary cultured astrocytes
T587 1278-1288 Entity denotes astrocytes
T586 501-529 Protein denotes of proinflammatory cytokines
T585 414-427 Protein denotes knockout mice
T584 784-802 Entity denotes Gelatin zymography
T567 12-19 Protein denotes of Nrf2
T574 86-119 Entity denotes Mouse Primary Cultured Astrocytes
T562 954-969 Entity denotes h after scratch
T563 840-847 Protein denotes of MMP9
T564 671-679 Protein denotes of NF-κB
T565 1072-1090 Protein denotes Nrf2 KO astrocytes
T566 748-752 Protein denotes IL-6
T568 865-870 Protein denotes NF-κB
T569 1214-1233 Protein denotes activation of NF-κB
T570 1072-1090 Entity denotes Nrf2 KO astrocytes
T571 1072-1076 Protein denotes Nrf2
T572 1104-1117 Entity denotes WT astrocytes
T573 758-762 Protein denotes MMP9
T575 477-485 Protein denotes of NF-κB
T576 741-753 Protein denotes IL-1β, IL-6,
T577 558-585 Entity denotes Primary cultured astrocytes
T578 707-711 Protein denotes mRNA
T579 302-306 Protein denotes Nrf2
T580 218-226 Protein denotes of NF-κB
T581 734-739 Protein denotes TNF-α
T582 716-723 Protein denotes protein
T583 886-914 Protein denotes of proinflammatory cytokines
R1725 T2026 T2031 themeOf "sterile PBS, cultured with complete culture medium","sterile PBS, cultured with complete culture medium"
R2676 T3059 T3095 themeOf The nuclear proteins,The nuclear proteins were extracted with buffer containing 20 mM HEPES
R8178 T9946 T10077 themeOf TNF-α,"that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch"
R8179 T9946 T10084 themeOf TNF-α,"that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch"
R8180 T9947 T10075 themeOf that NF-κB,that NF-κB is activated in brain and spinal cord after traumatic injury
R8182 T9950 T10087 themeOf IL-6,"the expression of TNF-α, IL-1β, IL-6, and MMP9 resulting in more cell deaths in astrocytes after scratch"
R8184 T9956 T10085 causeOf NF-κB,by NF-κB signaling
R8187 T9957 T10097 themeOf of MMP9,overexpression of MMP9
R8188 T9957 T10098 themeOf of MMP9,overexpression of MMP9
R8189 T9958 T10076 causeOf NF-κB,NF-κB signaling
R8192 T9960 T10064 themeOf of MMP9,transcription of MMP9
R8195 T9964 T10062 themeOf TNF-α,"Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9"
R8196 T9965 T10066 themeOf MMP9,"overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes"
R8197 T9965 T10071 themeOf MMP9,"overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes"
R8198 T9970 T10061 themeOf IL-1β,"Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9"
R8200 T9972 T10059 themeOf of NF-κB,"Enhanced activation of NF-κB is also discovered in lung, macrophages, and mouse embryonic fibroblasts"
R8203 T9974 T10070 themeOf TNF-α,"overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes"
R8204 T9974 T10073 themeOf TNF-α,"overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes"
R8207 T10014 T10074 themeOf of proinflammatory cytokines,expression of proinflammatory cytokines
R8208 T10015 T10055 themeOf of NF-κB,"activation of NF-κB [15, 16]"
R8209 T9986 T10101 themeOf IL-1β,"the expression of TNF-α, IL-1β, and IL-6 in cultured astrocytes"
R8210 T10017 T10090 themeOf IL-1β,"the expression of TNF-α, IL-1β, IL-6, and MMP9 resulting in more cell deaths in astrocytes after scratch"
R8211 T9989 T10088 themeOf TNF-α,"the expression of TNF-α, IL-1β, IL-6, and MMP9 resulting in more cell deaths in astrocytes after scratch"
R8212 T10018 T10099 themeOf TNF-α,"the expression of TNF-α, IL-1β, and IL-6 in cultured astrocytes"
R8213 T10022 T10089 themeOf MMP9,"the expression of TNF-α, IL-1β, IL-6, and MMP9 resulting in more cell deaths in astrocytes after scratch"
R8214 T9997 T10100 themeOf IL-6,"the expression of TNF-α, IL-1β, and IL-6 in cultured astrocytes"
R8215 T10024 T10079 themeOf MMP9 after scratch,"that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch"
R8216 T9999 T10086 causeOf NF-κB,that NF-κB can inhibit Nrf2 at transcriptional level
R8217 T10024 T10081 themeOf MMP9 after scratch,"that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch"
R8218 T10002 T10054 themeOf with DNA,NF-κB binds with DNA once it was activated
R8219 T10026 T10060 themeOf IL-6,"Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9"
R8220 T10028 T10063 themeOf MMP9,"Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9"
R8221 T10007 T10078 themeOf IL-6,"that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch"
R8222 T10007 T10082 themeOf IL-6,"that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch"
R8223 T10008 T10067 themeOf IL-1β,"overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes"
R8224 T10008 T10069 themeOf IL-1β,"overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes"
R8225 T10009 T10068 themeOf IL-6,"overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes"
R8226 T10009 T10072 themeOf IL-6,"overexpression of TNF-α, IL-1β, IL-6, and MMP9 in cultured astrocytes"
R8227 T10011 T10086 themeOf Nrf2,that NF-κB can inhibit Nrf2 at transcriptional level
R8228 T10030 T10065 themeOf of Nrf2 in brain tissue,of Nrf2 in brain tissue
R8229 T10031 T10080 themeOf IL-1β,"that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch"
R8230 T10031 T10083 themeOf IL-1β,"that overexpression of TNF-α, IL-1β, IL-6, and MMP9 after scratch"
R8231 T10034 T10054 themeOf NF-κB,NF-κB binds with DNA once it was activated
R8232 T10038 T10057 causeOf NF-κB activation induced by lipopolysaccharide (LPS) could be attenuated by,NF-κB activation induced by lipopolysaccharide (LPS) could be attenuated by
R8233 T10059 T10056 themeOf "Enhanced activation of NF-κB is also discovered in lung, macrophages, and mouse embryonic fibroblasts",Enhanced activation of NF-κB
R8234 T10060 T10094 themeOf "Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9","the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9"
R8235 T10061 T10095 themeOf "Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9","the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9"
R8236 T10062 T10092 themeOf "Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9","the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9"
R8237 T10063 T10093 themeOf "Our results demonstrated the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9","the upregulated expression of TNF-α, IL-1β, IL-6, and MMP9"
R8238 T10074 T10096 themeOf expression of proinflammatory cytokines,scratch injury induced higher activity of NF-κB and enhanced expression of proinflammatory cytokines
R439 T586 T595 themeOf of proinflammatory cytokines,expression of proinflammatory cytokines in a model of TBI
R448 T575 T594 themeOf of NF-κB,the activation of NF-κB
R449 T580 T593 themeOf of NF-κB,"activation of NF-κB in the brain after TBI,"
R450 T583 T592 themeOf of proinflammatory cytokines,expression of proinflammatory cytokines mentioned
R982 T1198 T1218 causeOf of Nrf2,we analysed the influence of Nrf2 on the expression of proinflammatory cytokines in primary cultured astrocytes from transgenic mice after scratch injury
R983 T1212 T1217 themeOf of proinflammatory cytokines,on the expression of proinflammatory cytokines in primary cultured astrocytes from transgenic mice after scratch injury
R984 T1217 T1218 themeOf on the expression of proinflammatory cytokines in primary cultured astrocytes from transgenic mice after scratch injury,we analysed the influence of Nrf2 on the expression of proinflammatory cytokines in primary cultured astrocytes from transgenic mice after scratch injury
R4551 T5347 T5355 themeOf of Nrf2,Disruption of Nrf2
R4552 T5353 T5354 themeOf that NF-κB,that NF-κB is activated in brain after TBI [2]
R4553 T5355 T5356 causeOf Disruption of Nrf2,Disruption of Nrf2 Enhanced Upregulation of NF-κB DNA-Binding Activity after Scratch Injury
R5361 T6377 T6414 themeOf IL-6 in cultured astrocytes at 24 ,IL-6 in cultured astrocytes at 24 
R5362 T6382 T6413 locationOf astrocytes after TBI,Nrf2 in astrocytes after TBI
R5363 T6389 T6411 themeOf TNF-α,"TNF-α, IL-1β, and IL-6 are regulated by NF-κB and reflect the endogenous activity of NF-κB"
R5364 T6395 T6410 themeOf IL-6,"TNF-α, IL-1β, and IL-6 are regulated by NF-κB and reflect the endogenous activity of NF-κB"
R5365 T6397 T6412 themeOf IL-1β,"TNF-α, IL-1β, and IL-6 are regulated by NF-κB and reflect the endogenous activity of NF-κB"
R5366 T6398 T6410 causeOf by NF-κB,"TNF-α, IL-1β, and IL-6 are regulated by NF-κB and reflect the endogenous activity of NF-κB"
R5367 T6398 T6411 causeOf by NF-κB,"TNF-α, IL-1β, and IL-6 are regulated by NF-κB and reflect the endogenous activity of NF-κB"
R5368 T6398 T6412 causeOf by NF-κB,"TNF-α, IL-1β, and IL-6 are regulated by NF-κB and reflect the endogenous activity of NF-κB"
R5369 T6407 T6413 themeOf Nrf2 in astrocytes after TBI,Nrf2 in astrocytes after TBI
R5370 T6409 T6414 locationOf cultured astrocytes,IL-6 in cultured astrocytes at 24 
R5808 T6999 T7009 themeOf of MMP9,Expression of MMP9

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T315 0-3 DT denotes The
T316 4-11 NN denotes Absence
T317 12-14 IN denotes of
T318 15-19 NNP denotes Nrf2
T319 20-28 NNP denotes Enhances
T320 29-44 NNP denotes NF-κB-Dependent
T321 45-57 NNP denotes Inflammation
T322 58-67 VBG denotes following
T323 68-75 NNP denotes Scratch
T324 76-82 NNP denotes Injury
T325 83-85 IN denotes in
T326 86-91 NNP denotes Mouse
T327 92-99 JJ denotes Primary
T328 100-108 NNP denotes Cultured
T329 109-119 NNPS denotes Astrocytes
T9677 20738-20739 . denotes .
T9676 20735-20738 NNP denotes TBI
T9675 20729-20734 IN denotes after
T9674 20721-20728 NN denotes therapy
T9673 20703-20720 JJ denotes anti-inflammatory
T9672 20699-20702 IN denotes for
T9671 20692-20698 NN denotes target
T9670 20682-20691 JJ denotes important
T9669 20679-20681 DT denotes an
T9668 20676-20678 VB denotes be
T9667 20672-20675 MD denotes may
T9666 20667-20671 NNP denotes Nrf2
T9665 20662-20666 IN denotes that
T9664 20654-20661 VBP denotes suggest
T9663 20646-20653 NNS denotes results
T9662 20640-20645 DT denotes These
T9661 20638-20639 . denotes .
T9660 20632-20638 NN denotes injury
T9659 20624-20631 NN denotes scratch
T9658 20618-20623 IN denotes after
T9657 20607-20617 NNS denotes astrocytes
T9656 20604-20606 IN denotes in
T9655 20597-20603 NNS denotes deaths
T9654 20592-20596 NN denotes cell
T9653 20587-20591 JJR denotes more
T9652 20584-20586 IN denotes in
T9651 20574-20583 VBG denotes resulting
T9650 20569-20573 NNP denotes MMP9
T9649 20565-20568 CC denotes and
T9648 20563-20564 , denotes ,
T9647 20559-20563 NNP denotes IL-6
T9646 20557-20558 , denotes ,
T9645 20552-20557 NNP denotes IL-1β
T9644 20550-20551 , denotes ,
T9643 20545-20550 NNP denotes TNF-α
T9642 20542-20544 IN denotes of
T9641 20531-20541 NN denotes expression
T9640 20527-20530 DT denotes the
T9639 20523-20526 CC denotes and
T9638 20517-20522 NNP denotes NF-κB
T9637 20514-20516 IN denotes of
T9636 20503-20513 NN denotes activation
T9635 20499-20502 DT denotes the
T9634 20491-20498 VBD denotes induced
T9439 19400-19402 VB denotes be
T9438 19396-19399 MD denotes may
T9437 19390-19395 WDT denotes which
T9436 19388-19389 , denotes ,
T9435 19384-19388 NNP denotes Nrf2
T9434 19380-19383 CC denotes and
T9433 19374-19379 NNP denotes NF-κB
T9432 19366-19373 IN denotes between
T9431 19356-19365 NN denotes crosstalk
T9430 19345-19355 VBP denotes complicate
T9429 19335-19344 JJ denotes potential
T9428 19331-19334 DT denotes the
T9427 19322-19330 VBP denotes indicate
T9426 19313-19321 NNS denotes findings
T9425 19307-19312 DT denotes these
T9424 19303-19306 PDT denotes All
T9423 19301-19302 . denotes .
T9422 19300-19301 NNP denotes ]
T9421 19298-19300 CD denotes 25
T9420 19297-19298 NNP denotes [
T9419 19281-19296 NN denotes hypoacetylation
T9418 19273-19280 NN denotes histone
T9417 19267-19272 JJ denotes local
T9416 19264-19266 CC denotes or
T9415 19260-19263 NNP denotes CBP
T9414 19257-19259 IN denotes of
T9413 19250-19256 NN denotes domain
T9412 19242-19249 NNP denotes CH1-KIX
T9410 19233-19237 IN denotes with
T9409 19221-19232 NN denotes interaction
T9408 19209-19220 JJ denotes competitive
T9407 19206-19208 IN denotes by
T9406 19200-19205 NN denotes level
T9405 19184-19199 JJ denotes transcriptional
T9404 19181-19183 IN denotes at
T9403 19173-19180 NN denotes pathway
T9402 19164-19172 JJ denotes Nrf2-ARE
T9401 19160-19163 DT denotes the
T9400 19150-19159 VBD denotes repressed
T9399 19142-19149 NN denotes subunit
T9398 19138-19141 CD denotes p65
T9397 19132-19137 NNP denotes NF-κB
T9396 19130-19131 . denotes .
T9395 19125-19130 NN denotes level
T9394 19109-19124 JJ denotes transcriptional
T9393 19106-19108 IN denotes at
T9392 19101-19105 NNP denotes Nrf2
T9391 19093-19100 VB denotes inhibit
T9390 19089-19092 MD denotes can
T9389 19083-19088 NNP denotes NF-κB
T9388 19078-19082 IN denotes that
T9387 19069-19077 VBN denotes reported
T9386 19066-19068 VBZ denotes is
T9385 19063-19065 PRP denotes it
T9384 19061-19062 , denotes ,
T9383 19048-19061 RB denotes Interestingly
T9382 19046-19047 . denotes .
T9381 19045-19046 NNP denotes ]
T9380 19043-19045 CD denotes 24
T9379 19042-19043 NNP denotes [
T9378 19035-19041 NN denotes sepsis
T9377 19022-19034 JJ denotes experimental
T9376 19016-19021 IN denotes after
T9375 19011-19015 NNS denotes mice
T9374 18996-19010 JJ denotes Nrf2-deficient
T9373 18993-18995 IN denotes of
T9372 18981-18992 NNS denotes fibroblasts
T9371 18971-18980 JJ denotes embryonic
T9370 18965-18970 NN denotes mouse
T9369 18961-18964 CC denotes and
T9368 18959-18960 , denotes ,
T9367 18948-18959 NNS denotes macrophages
T9366 18946-18947 , denotes ,
T9365 18942-18946 NN denotes lung
T9364 18939-18941 IN denotes in
T9363 18928-18938 VBN denotes discovered
T9362 18923-18927 RB denotes also
T9361 18920-18922 VBZ denotes is
T9360 18914-18919 NNP denotes NF-κB
T9359 18911-18913 IN denotes of
T9358 18900-18910 NN denotes activation
T9357 18891-18899 VBN denotes Enhanced
T9356 18889-18890 . denotes .
T9355 18883-18889 NN denotes injury
T9354 18875-18882 NN denotes scratch
T9353 18869-18874 IN denotes after
T9352 18858-18868 NNS denotes astrocytes
T9351 18849-18857 JJ denotes cultured
T9350 18846-18848 IN denotes in
T9349 18838-18845 VBD denotes existed
T9348 18825-18837 NN denotes relationship
T9347 18820-18824 JJ denotes such
T9346 18810-18819 VBD denotes confirmed
T9345 18802-18809 JJ denotes further
T9344 18796-18801 NN denotes study
T9343 18791-18795 DT denotes this
T9342 18786-18790 IN denotes from
T9341 18778-18785 NNS denotes Results
T9340 18776-18777 . denotes .
T9339 18775-18776 NNP denotes ]
T9338 18773-18775 CD denotes 23
T9337 18771-18772 , denotes ,
T9336 18770-18771 CD denotes 5
T9335 18768-18769 , denotes ,
T9334 18767-18768 CD denotes 4
T9333 18766-18767 NNP denotes [
T9332 18762-18765 NNP denotes TBI
T9331 18756-18761 IN denotes after
T9330 18746-18755 JJ denotes intestine
T9329 18742-18745 CC denotes and
T9328 18740-18741 , denotes ,
T9327 18735-18740 NN denotes brain
T9326 18733-18734 , denotes ,
T9325 18729-18733 NN denotes lung
T9324 18726-18728 IN denotes in
T9323 18717-18725 NN denotes response
T9322 18704-18716 JJ denotes inflammatory
T9321 18700-18703 CC denotes and
T9320 18691-18699 NN denotes activity
T9319 18685-18690 JJ denotes NF-κB
T9318 18682-18684 IN denotes of
T9317 18669-18681 NN denotes augmentation
T9316 18661-18668 VBZ denotes induces
T9315 18656-18660 JJ denotes Nrf2
T9314 18653-18655 IN denotes of
T9313 18643-18652 NN denotes depletion
T9312 18638-18642 IN denotes that
T9311 18629-18637 VBP denotes indicate
T9310 18621-18628 NNS denotes studies
T9309 18612-18620 JJ denotes previous
T9308 18608-18611 PRP$ denotes our
T9307 18606-18607 , denotes ,
T9306 18595-18606 RB denotes Furthermore
T9305 18593-18594 . denotes .
T9304 18592-18593 NNP denotes ]
T9303 18590-18592 CD denotes 22
T9302 18589-18590 NNP denotes [
T9301 18587-18588 -RRB- denotes )
T9300 18584-18587 NNP denotes CUR
T9299 18583-18584 -LRB- denotes (
T9298 18574-18582 NN denotes curcumin
T9297 18570-18573 CC denotes and
T9296 18566-18569 NNP denotes SFN
T9295 18563-18565 IN denotes as
T9294 18558-18562 JJ denotes such
T9293 18556-18557 , denotes ,
T9292 18546-18556 NNS denotes activators
T9291 18541-18545 JJ denotes Nrf2
T9290 18533-18540 JJ denotes diverse
T9289 18530-18532 IN denotes by
T9288 18519-18529 VBN denotes attenuated
T9287 18516-18518 VB denotes be
T9286 18510-18515 MD denotes could
T9285 18508-18509 -RRB- denotes )
T9284 18505-18508 NNP denotes LPS
T9283 18504-18505 -LRB- denotes (
T9282 18485-18503 NN denotes lipopolysaccharide
T9281 18482-18484 IN denotes by
T9280 18474-18481 VBN denotes induced
T9279 18463-18473 NN denotes activation
T9278 18457-18462 JJ denotes NF-κB
T9277 18453-18456 CC denotes And
T9276 18451-18452 . denotes .
T9275 18450-18451 NNP denotes ]
T9274 18448-18450 CD denotes 21
T9273 18447-18448 NNP denotes [
T9272 18437-18446 VBG denotes signaling
T9271 18431-18436 JJ denotes NF-κB
T9270 18419-18430 VBG denotes alleviating
T9269 18406-18418 RB denotes consequently
T9268 18404-18405 , denotes ,
T9267 18391-18404 NN denotes translocation
T9266 18383-18390 JJ denotes nuclear
T9265 18375-18382 NN denotes subunit
T9264 18369-18374 JJ denotes NF-κB
T9263 18365-18368 CD denotes p65
T9262 18361-18364 CC denotes and
T9261 18345-18360 NN denotes phosphorylation
T9260 18342-18344 NNP denotes κB
T9259 18337-18342 NNP denotes IKK/I
T9258 18329-18336 VB denotes inhibit
T9257 18326-18328 TO denotes to
T9256 18321-18325 JJ denotes able
T9255 18318-18320 VB denotes be
T9254 18315-18317 TO denotes to
T9253 18309-18314 VBN denotes found
T9252 18306-18308 VBZ denotes is
T9251 18302-18305 NNP denotes SFN
T9250 18299-18301 IN denotes of
T9249 18284-18298 NNP denotes Administration
T9248 18282-18283 . denotes .
T9247 18273-18282 VBG denotes signaling
T9246 18267-18272 JJ denotes NF-κB
T9245 18264-18266 IN denotes by
T9244 18255-18263 VBN denotes mediated
T9243 18251-18254 VBP denotes are
T9242 18245-18250 WDT denotes which
T9241 18236-18244 NNS denotes pathways
T9240 18220-18235 JJ denotes proinflammatory
T9239 18217-18219 IN denotes of
T9238 18205-18216 NN denotes suppression
T9237 18202-18204 IN denotes by
T9236 18193-18201 VBN denotes achieved
T9235 18190-18192 VB denotes be
T9234 18186-18189 MD denotes may
T9233 18181-18185 NNP denotes Nrf2
T9232 18178-18180 IN denotes of
T9231 18171-18177 NN denotes effect
T9230 18159-18170 JJ denotes antioxidant
T9229 18154-18158 WDT denotes that
T9228 18142-18153 NN denotes possibility
T9227 18138-18141 DT denotes the
T9226 18130-18137 VBP denotes suggest
T9225 18122-18129 NNS denotes studies
T9224 18115-18121 JJ denotes animal
T9114 17487-17490 DT denotes the
T9113 17477-17486 VBZ denotes decreases
T9112 17473-17476 CC denotes and
T9111 17469-17472 NN denotes rat
T9110 17466-17468 IN denotes of
T9109 17459-17465 NN denotes memory
T9108 17451-17458 JJ denotes spatial
T9107 17442-17450 VBZ denotes improves
T9106 17428-17441 RB denotes significantly
T9105 17426-17427 , denotes ,
T9104 17422-17426 NNP denotes Nrf2
T9103 17419-17421 IN denotes of
T9102 17411-17418 NN denotes inducer
T9101 17408-17410 DT denotes an
T9100 17406-17407 , denotes ,
T9099 17403-17406 NNP denotes SFN
T9098 17400-17402 IN denotes of
T9097 17385-17399 NN denotes administration
T9096 17374-17384 NNP denotes Postinjury
T9095 17372-17373 . denotes .
T9094 17371-17372 NNP denotes ]
T9093 17369-17371 CD denotes 18
T9092 17368-17369 NNP denotes [
T9091 17362-17367 NN denotes brain
T9090 17359-17361 IN denotes in
T9089 17352-17358 NN denotes damage
T9088 17348-17351 DT denotes the
T9087 17341-17347 VB denotes reduce
T9086 17328-17340 RB denotes subsequently
T9085 17322-17327 MD denotes would
T9084 17316-17321 WDT denotes which
T9083 17314-17315 , denotes ,
T9082 17313-17314 -RRB- denotes )
T9081 17309-17313 CD denotes NQO1
T9080 17308-17309 -LRB- denotes (
T9079 17291-17307 JJ denotes oxidoreductase-1
T9078 17283-17290 VB denotes quinone
T9077 17281-17282 : denotes :
T9076 17280-17281 NNP denotes H
T9075 17279-17280 -RRB- denotes )
T9074 17278-17279 NNP denotes P
T9073 17277-17278 -LRB- denotes (
T9072 17273-17276 NNP denotes NAD
T9071 17269-17272 CC denotes and
T9070 17267-17268 -RRB- denotes )
T9069 17263-17267 JJ denotes HO-1
T9068 17262-17263 -LRB- denotes (
T9067 17250-17261 JJ denotes oxygenase-1
T9066 17245-17249 NN denotes heme
T9065 17242-17244 IN denotes as
T9064 17237-17241 JJ denotes such
T9063 17235-17236 , denotes ,
T9062 17230-17235 NNS denotes genes
T9061 17218-17229 JJ denotes antioxidant
T9060 17215-17217 IN denotes of
T9059 17209-17214 NN denotes group
T9058 17207-17208 DT denotes a
T9057 17204-17206 IN denotes of
T9056 17190-17203 NN denotes transcription
T9055 17180-17189 NNS denotes activates
T9054 17175-17179 JJ denotes Nrf2
T9053 17172-17174 IN denotes of
T9052 17166-17171 NN denotes level
T9051 17157-17165 JJ denotes Enhanced
T9050 17155-17156 . denotes .
T9049 17154-17155 NNP denotes ]
T9048 17152-17154 CD denotes 17
T9047 17151-17152 NNP denotes [
T9046 17147-17150 NNP denotes TBI
T9045 17141-17146 IN denotes after
T9044 17134-17140 NN denotes tissue
T9043 17128-17133 NN denotes brain
T9042 17125-17127 IN denotes in
T9041 17120-17124 NNP denotes Nrf2
T9040 17117-17119 IN denotes of
T9039 17104-17116 NN denotes augmentation
T9038 17100-17103 DT denotes the
T9037 17093-17099 VBN denotes proved
T9036 17089-17092 VBZ denotes has
T9035 17083-17088 NN denotes study
T9034 17074-17082 JJ denotes previous
T9033 17070-17073 PRP$ denotes Our
T9032 17068-17069 . denotes .
T9031 17065-17068 NNP denotes TBI
T9030 17062-17064 IN denotes in
T9029 17053-17061 VBN denotes involved
T9028 17049-17052 DT denotes all
T9027 17045-17048 VBP denotes are
T9026 17039-17044 WDT denotes which
T9025 17037-17038 , denotes ,
T9024 17026-17037 NNS denotes metabolites
T9023 17020-17025 JJ denotes toxic
T9022 17017-17019 IN denotes of
T9021 17004-17016 NN denotes accumulation
T9020 17000-17003 CC denotes and
T9019 16998-16999 , denotes ,
T9018 16992-16998 NN denotes damage
T9017 16979-16991 JJ denotes inflammatory
T9016 16977-16978 , denotes ,
T9015 16971-16977 NN denotes stress
T9014 16961-16970 JJ denotes oxidative
T9013 16952-16960 VBG denotes reducing
T9012 16949-16951 IN denotes in
T9011 16939-16948 NN denotes regulator
T9010 16935-16938 JJ denotes key
T9009 16931-16934 DT denotes the
T9008 16928-16930 VB denotes be
T9007 16925-16927 TO denotes to
T9006 16918-16924 VBN denotes proved
T9005 16913-16917 VBN denotes been
T9004 16909-16912 VBZ denotes has
T9003 16901-16908 NN denotes pathway
T9002 16892-16900 JJ denotes Nrf2-ARE
T9001 16890-16891 . denotes .
T9000 16887-16890 NNP denotes TBI
T8999 16881-16886 IN denotes after
T8998 16876-16880 NN denotes loop
T8997 16861-16875 JJ denotes autoregulatory
T8996 16856-16860 JJ denotes such
T8995 16848-16855 VB denotes prevent
T8994 16845-16847 TO denotes to
T8993 16838-16844 NN denotes factor
T8992 16829-16837 JJ denotes upstream
T8991 16826-16828 DT denotes an
T8990 16823-16825 IN denotes on
T8989 16813-16822 VBG denotes targeting
T8988 16809-16812 IN denotes for
T8987 16802-16808 NN denotes choice
T8986 16797-16801 JJ denotes good
T8985 16795-16796 DT denotes a
T8984 16792-16794 VBZ denotes is
T8983 16789-16791 PRP denotes It
T8982 16787-16788 . denotes .
T8981 16782-16787 NN denotes brain
T8980 16779-16781 TO denotes to
T8979 16772-16778 NN denotes injury
T8978 16762-16771 JJ denotes secondary
T8977 16754-16761 VBZ denotes induces
T8976 16750-16753 CC denotes and
T8975 16737-16749 NN denotes inflammation
T8974 16734-16736 IN denotes of
T8973 16727-16733 NN denotes effect
T8972 16718-16726 JJ denotes damaging
T8971 16714-16717 DT denotes the
T8970 16703-16713 VBZ denotes aggravates
T8969 16693-16702 RB denotes extremely
T8968 16688-16692 NN denotes loop
T8967 16673-16687 JJ denotes autoregulatory
T8966 16668-16672 DT denotes This
T8965 16666-16667 . denotes .
T8964 16665-16666 NN denotes ]
T8963 16663-16665 CD denotes 16
T8962 16661-16662 , denotes ,
T8961 16659-16661 CD denotes 15
T8960 16658-16659 NNP denotes [
T8959 16652-16657 NNP denotes NF-κB
T8958 16649-16651 IN denotes of
T8957 16638-16648 NN denotes activation
T8956 16631-16637 VB denotes induce
T8955 16627-16630 MD denotes can
T8954 16622-16626 RB denotes also
T8953 16616-16621 NNP denotes IL-1β
T8952 16612-16615 CC denotes and
T8951 16606-16611 NNP denotes TNF-α
T8950 16602-16605 CC denotes and
T8949 16600-16601 , denotes ,
T8948 16599-16600 NNP denotes ]
T8947 16597-16599 CD denotes 14
T8946 16596-16597 NNP denotes [
T8945 16590-16595 NNS denotes cells
T8944 16580-16589 NN denotes carcinoma
T8943 16574-16579 NN denotes colon
T8942 16568-16573 JJ denotes human
T8941 16565-16567 IN denotes in
T8940 16560-16564 NNP denotes MMP9
T8939 16557-16559 IN denotes of
T8938 16542-16556 NN denotes overexpression
T8937 16534-16541 VBZ denotes induces
T8936 16529-16533 NNP denotes IL-6
T8935 16525-16528 CC denotes and
T8934 16523-16524 , denotes ,
T8933 16522-16523 NNP denotes ]
T8932 16520-16522 CD denotes 13
T8931 16518-16519 , denotes ,
T8930 16516-16518 CD denotes 12
T8929 16515-16516 NNP denotes [
T8928 16504-16514 NNS denotes astrocytes
T8927 16501-16503 IN denotes in
T8926 16496-16500 NNP denotes MMP9
T8925 16492-16495 IN denotes for
T9629 20461-20471 NN denotes conclusion
T9628 20458-20460 IN denotes In
T9627 20456-20457 . denotes .
T9626 20452-20456 NN denotes time
T9625 20446-20451 JJ denotes first
T9624 20442-20445 DT denotes the
T9623 20438-20441 IN denotes for
T9622 20431-20437 NN denotes injury
T9621 20423-20430 NN denotes scratch
T9620 20417-20422 IN denotes after
T9619 20406-20416 NNS denotes astrocytes
T9618 20397-20405 NN denotes knockout
T9617 20392-20396 NNP denotes Nrf2
T9616 20389-20391 IN denotes in
T9615 20384-20388 NNP denotes MMP9
T9614 20380-20383 CC denotes and
T9613 20378-20379 , denotes ,
T9612 20374-20378 NNP denotes IL-6
T9611 20372-20373 , denotes ,
T9610 20367-20372 NNP denotes IL-1β
T9609 20365-20366 , denotes ,
T9608 20360-20365 NNP denotes TNF-α
T9607 20357-20359 IN denotes of
T9606 20346-20356 NN denotes expression
T9605 20334-20345 JJ denotes upregulated
T9604 20330-20333 DT denotes the
T9603 20317-20329 VBD denotes demonstrated
T9602 20309-20316 NNS denotes results
T9601 20305-20308 PRP$ denotes Our
T9600 20303-20304 . denotes .
T9599 20300-20303 NNP denotes TBI
T9598 20294-20299 IN denotes after
T9597 20284-20293 NNS denotes mediators
T9596 20268-20283 JJ denotes proinflammatory
T9595 20264-20267 CC denotes and
T9594 20262-20263 , denotes ,
T9593 20258-20262 NNP denotes Nrf2
T9592 20256-20257 , denotes ,
T9591 20246-20256 NNS denotes astrocytes
T9590 20240-20245 IN denotes among
T9589 20227-20239 NN denotes relationship
T9588 20223-20226 DT denotes the
T9587 20220-20222 IN denotes on
T9586 20212-20219 VBD denotes focused
T9585 20206-20211 NN denotes study
T9584 20203-20205 DT denotes no
T9583 20200-20202 VBZ denotes is
T9582 20194-20199 EX denotes there
T9581 20192-20193 , denotes ,
T9580 20189-20192 RB denotes now
T9579 20184-20188 IN denotes till
T9578 20180-20183 CC denotes But
T9577 20178-20179 . denotes .
T9576 20172-20178 NN denotes injury
T9575 20166-20171 NN denotes brain
T9574 20160-20165 IN denotes after
T9573 20152-20159 NN denotes process
T9572 20139-20151 JJ denotes inflammatory
T9571 20136-20138 IN denotes in
T9570 20125-20135 NNS denotes astrocytes
T9569 20122-20124 IN denotes of
T9568 20117-20121 NN denotes role
T9567 20113-20116 DT denotes the
T9566 20104-20112 VBP denotes indicate
T9565 20096-20103 NNS denotes results
T9564 20090-20095 DT denotes Those
T9563 20088-20089 . denotes .
T9562 20087-20088 NNP denotes ]
T9561 20085-20087 CD denotes 29
T9560 20084-20085 NNP denotes [
T9559 20063-20083 NN denotes immunohistochemistry
T9558 20060-20062 IN denotes by
T9557 20052-20059 NNS denotes tissues
T9556 20046-20051 NN denotes brain
T9555 20043-20045 IN denotes in
T9554 20034-20042 VBN denotes observed
T9553 20029-20033 VBD denotes were
T9552 20018-20028 NNS denotes astrocytes
T9551 20004-20017 JJ denotes MMP9-positive
T9550 20002-20003 , denotes ,
T9549 19993-20002 NN denotes occlusion
T9548 19986-19992 NN denotes artery
T9547 19977-19985 JJ denotes cerebral
T9546 19970-19976 JJ denotes middle
T9545 19964-19969 IN denotes after
T9544 19959-19963 IN denotes that
T9543 19950-19958 VBD denotes revealed
T9542 19944-19949 NN denotes study
T9541 19936-19943 DT denotes Another
T9540 19934-19935 . denotes .
T9539 19933-19934 NN denotes ]
T9538 19931-19933 CD denotes 28
T9537 19929-19930 , denotes ,
T9536 19927-19929 CD denotes 27
T9535 19926-19927 NNP denotes [
T9534 19912-19925 VBN denotes oxyhemoglobin
T9533 19909-19911 CC denotes or
T9532 19905-19908 NNP denotes LPS
T9531 19900-19904 IN denotes with
T9530 19890-19899 NN denotes treatment
T9529 19884-19889 IN denotes after
T9528 19873-19883 NNS denotes astrocytes
T9527 19864-19872 JJ denotes cultured
T9526 19861-19863 IN denotes in
T9525 19856-19860 NNP denotes IL-6
T9524 19852-19855 CC denotes and
T9523 19850-19851 , denotes ,
T9522 19845-19850 NNP denotes IL-1β
T9521 19843-19844 , denotes ,
T9520 19838-19843 NNP denotes TNF-α
T9519 19835-19837 IN denotes of
T9518 19824-19834 NN denotes expression
T9517 19820-19823 DT denotes the
T9516 19809-19819 VBD denotes identified
T9515 19803-19808 NN denotes study
T9514 19794-19802 JJ denotes Previous
T9513 19792-19793 . denotes .
T9512 19789-19792 NNP denotes TBI
T9511 19783-19788 IN denotes after
T9510 19775-19782 NN denotes pathway
T9509 19766-19774 JJ denotes Nrf2-ARE
T9508 19763-19765 IN denotes of
T9507 19752-19762 NN denotes activation
T9506 19749-19751 IN denotes in
T9505 19743-19748 NNS denotes cells
T9504 19732-19742 NN denotes respondent
T9503 19729-19731 IN denotes of
T9502 19724-19728 NN denotes kind
T9501 19720-19723 CD denotes one
T9500 19717-19719 VB denotes be
T9499 19713-19716 MD denotes may
T9498 19702-19712 NNS denotes astrocytes
T9497 19697-19701 IN denotes that
T9496 19688-19696 VBN denotes reasoned
T9495 19685-19687 VB denotes be
T9494 19681-19684 MD denotes can
T9493 19678-19680 PRP denotes it
T9492 19676-19677 , denotes ,
T9491 19669-19676 NN denotes nucleus
T9490 19666-19668 IN denotes in
T9489 19659-19665 RB denotes mainly
T9488 19650-19658 NN denotes function
T9487 19645-19649 VB denotes take
T9486 19638-19644 MD denotes should
T9485 19632-19637 WDT denotes which
T9484 19625-19631 NN denotes factor
T9483 19611-19624 NN denotes transcription
T9482 19609-19610 DT denotes a
T9481 19606-19608 VBZ denotes is
T9480 19601-19605 NNP denotes Nrf2
T9479 19596-19600 IN denotes that
T9478 19591-19595 NN denotes fact
T9477 19587-19590 DT denotes the
T9476 19584-19586 IN denotes of
T9475 19579-19583 NN denotes view
T9474 19576-19578 IN denotes In
T9473 19574-19575 . denotes .
T9472 19573-19574 NNP denotes ]
T9471 19571-19573 CD denotes 18
T9470 19570-19571 NNP denotes [
T9469 19566-19569 NNP denotes TBI
T9468 19560-19565 IN denotes after
T9467 19549-19559 NNS denotes astrocytes
T9466 19546-19548 IN denotes of
T9465 19538-19545 NN denotes nucleus
T9464 19535-19537 IN denotes in
T9463 19526-19534 VBN denotes detected
T9462 19519-19525 RB denotes mainly
T9461 19516-19518 VBZ denotes is
T9460 19511-19515 NNP denotes Nrf2
T9459 19506-19510 IN denotes that
T9458 19496-19505 VBN denotes confirmed
T9457 19491-19495 VBN denotes been
T9456 19487-19490 VBZ denotes has
T9455 19484-19486 PRP denotes It
T9454 19482-19483 . denotes .
T9453 19481-19482 NNP denotes ]
T9452 19479-19481 CD denotes 26
T9451 19478-19479 NNP denotes [
T9450 19470-19477 VBP denotes pathway
T9449 19468-19469 -RRB- denotes )
T9448 19463-19468 NNS denotes MAPKs
T9447 19462-19463 -LRB- denotes (
T9446 19455-19461 NN denotes kinase
T9445 19447-19454 NN denotes protein
T9444 19429-19446 JJ denotes mitogen-activated
T9443 19420-19428 JJ denotes upstream
T9442 19416-19419 DT denotes the
T9441 19413-19415 IN denotes by
T9440 19403-19412 VBN denotes regulated
T8924 16480-16491 NNS denotes stimulators
T8923 16473-16479 JJ denotes potent
T8922 16469-16472 VBP denotes are
T8921 16463-16468 NNP denotes IL-1β
T8920 16459-16462 CC denotes and
T8919 16453-16458 NNP denotes TNF-α
T8918 16451-16452 , denotes ,
T8917 16444-16451 NN denotes example
T8916 16440-16443 IN denotes For
T8915 16438-16439 . denotes .
T8914 16429-16438 NNS denotes mediators
T8913 16413-16428 JJ denotes proinflammatory
T8912 16407-16412 DT denotes these
T8911 16401-16406 IN denotes among
T8910 16396-16400 NN denotes loop
T8909 16381-16395 JJ denotes autoregulatory
T8908 16378-16380 DT denotes an
T8907 16375-16377 VBZ denotes is
T8906 16369-16374 EX denotes there
T8905 16364-16368 IN denotes that
T8904 16351-16363 VBN denotes demonstrated
T8903 16346-16350 VBN denotes been
T8902 16342-16345 VBZ denotes has
T8901 16339-16341 PRP denotes It
T8900 16337-16338 . denotes .
T8899 16327-16337 NNS denotes astrocytes
T8832 15955-15960 JJ denotes NF-κB
T8831 15953-15954 , denotes ,
T8830 15947-15953 NN denotes factor
T8829 15936-15946 NN denotes transcript
T8828 15934-15935 DT denotes a
T8827 15931-15933 IN denotes As
T8826 15929-15930 . denotes .
T8825 15928-15929 NN denotes ]
T8824 15927-15928 CD denotes 9
T8823 15925-15926 , denotes ,
T8822 15924-15925 CD denotes 2
T8821 15923-15924 NN denotes [
T8820 15916-15922 NN denotes injury
T8819 15906-15915 JJ denotes traumatic
T8818 15900-15905 IN denotes after
T8817 15895-15899 NN denotes cord
T8816 15888-15894 JJ denotes spinal
T8815 15884-15887 CC denotes and
T8814 15878-15883 NN denotes brain
T8813 15875-15877 IN denotes in
T8812 15865-15874 VBN denotes activated
T8811 15862-15864 VBZ denotes is
T8810 15856-15861 NNP denotes NF-κB
T8809 15851-15855 IN denotes that
T8808 15838-15850 VBN denotes demonstrated
T8807 15833-15837 VBN denotes been
T8806 15829-15832 VBZ denotes has
T8805 15826-15828 PRP denotes It
T8804 15824-15825 . denotes .
T8803 15820-15824 NN denotes time
T8802 15814-15819 JJ denotes first
T8801 15810-15813 DT denotes the
T8800 15806-15809 IN denotes for
T8799 15795-15805 NNS denotes astrocytes
T8798 15786-15794 NN denotes wildtype
T8797 15783-15785 IN denotes in
T8796 15777-15782 DT denotes those
T8795 15772-15776 IN denotes than
T8794 15761-15771 NNS denotes astrocytes
T8793 15752-15760 VBD denotes cultured
T8792 15743-15751 NN denotes knockout
T8791 15738-15742 JJ denotes Nrf2
T8790 15735-15737 IN denotes in
T8789 15725-15734 NNS denotes cytokines
T8788 15709-15724 JJ denotes proinflammatory
T8787 15706-15708 IN denotes of
T8786 15695-15705 NN denotes expression
T8785 15686-15694 JJ denotes enhanced
T8784 15682-15685 CC denotes and
T8783 15676-15681 NNP denotes NF-κB
T8782 15673-15675 IN denotes of
T8781 15664-15672 NN denotes activity
T8780 15657-15663 JJR denotes higher
T8779 15649-15656 VBD denotes induced
T8778 15642-15648 NN denotes injury
T8777 15634-15641 NN denotes scratch
T8776 15629-15633 IN denotes that
T8775 15620-15628 VBD denotes revealed
T8774 15617-15619 PRP denotes we
T8773 15612-15616 RB denotes Also
T8772 15610-15611 . denotes .
T8771 15600-15610 NNS denotes astrocytes
T8770 15591-15599 JJ denotes cultured
T8769 15588-15590 IN denotes in
T8768 15583-15587 NNP denotes MMP9
T8767 15579-15582 CC denotes and
T8766 15577-15578 , denotes ,
T8765 15573-15577 NNP denotes IL-6
T8764 15571-15572 , denotes ,
T8763 15566-15571 NNP denotes IL-1β
T8762 15564-15565 , denotes ,
T8761 15559-15564 NNP denotes TNF-α
T8760 15556-15558 IN denotes of
T8759 15541-15555 NN denotes overexpression
T8758 15537-15540 CC denotes and
T8757 15528-15536 NN denotes activity
T8756 15520-15527 JJ denotes binding
T8755 15516-15519 NNP denotes DNA
T8754 15510-15515 NNP denotes NF-κB
T8753 15507-15509 IN denotes of
T8752 15494-15506 NN denotes upregulation
T8751 15490-15493 DT denotes the
T8750 15482-15489 VBD denotes induced
T8749 15475-15481 NN denotes injury
T8748 15467-15474 NN denotes scratch
T8747 15462-15466 IN denotes that
T8746 15449-15461 VBD denotes demonstrated
T8745 15443-15448 NN denotes study
T8744 15435-15442 JJ denotes present
T8743 15431-15434 DT denotes The
T9633 20486-20490 JJ denotes Nrf2
T9632 20483-20485 IN denotes of
T9631 20473-20482 NN denotes depletion
T9630 20471-20472 , denotes ,
T9223 18110-18114 IN denotes from
T9222 18101-18109 VBD denotes obtained
T9221 18096-18100 NNP denotes Data
T9220 18094-18095 . denotes .
T9219 18088-18094 NNS denotes deaths
T9218 18077-18087 NNS denotes astrocytes
T9217 18072-18076 JJR denotes more
T9216 18069-18071 TO denotes to
T9215 18065-18068 VBD denotes led
T9214 18055-18064 NNS denotes mediators
T9213 18039-18054 JJ denotes proinflammatory
T9212 18033-18038 DT denotes these
T9211 18030-18032 IN denotes of
T9210 18015-18029 NN denotes overexpression
T9209 18011-18014 CC denotes and
T9208 18009-18010 , denotes ,
T9207 18005-18009 NN denotes time
T9206 17999-18004 JJ denotes first
T9205 17995-17998 DT denotes the
T9204 17991-17994 IN denotes for
T9203 17979-17990 NNS denotes astrocyetes
T9202 17970-17978 NN denotes wildtype
T9201 17967-17969 IN denotes in
T9200 17962-17966 IN denotes than
T9199 17951-17961 NNS denotes astrocytes
T9198 17942-17950 NN denotes knockout
T9197 17937-17941 JJ denotes Nrf2
T9196 17928-17936 JJ denotes cultured
T9195 17925-17927 IN denotes in
T9194 17914-17924 VBN denotes aggravated
T9193 17909-17913 RBR denotes more
T9192 17905-17908 VBD denotes was
T9191 17898-17904 NN denotes injury
T9190 17890-17897 NN denotes scratch
T9189 17884-17889 IN denotes after
T9188 17879-17883 NNP denotes MMP9
T9187 17875-17878 CC denotes and
T9186 17873-17874 , denotes ,
T9185 17869-17873 NNP denotes IL-6
T9184 17867-17868 , denotes ,
T9183 17862-17867 NNP denotes IL-1β
T9182 17860-17861 , denotes ,
T9181 17855-17860 NNP denotes TNF-α
T9180 17852-17854 IN denotes of
T9179 17837-17851 NN denotes overexpression
T9178 17832-17836 IN denotes that
T9177 17823-17831 VBD denotes revealed
T9176 17815-17822 NNS denotes results
T9175 17811-17814 PRP$ denotes our
T9174 17809-17810 , denotes ,
T9173 17805-17809 RB denotes Here
T9172 17803-17804 . denotes .
T9171 17802-17803 NNP denotes ]
T9170 17800-17802 CD denotes 20
T9169 17799-17800 NNP denotes [
T9168 17795-17798 NNP denotes TBI
T9167 17789-17794 IN denotes after
T9166 17783-17788 NN denotes brain
T9165 17780-17782 IN denotes in
T9164 17770-17779 NNS denotes mediators
T9163 17754-17769 JJ denotes proinflammatory
T9162 17751-17753 IN denotes of
T9161 17740-17750 NN denotes expression
T9160 17733-17739 JJR denotes higher
T9159 17725-17732 VBZ denotes induces
T9158 17720-17724 JJ denotes Nrf2
T9157 17717-17719 IN denotes of
T9156 17707-17716 NN denotes Depletion
T9155 17705-17706 . denotes .
T9154 17702-17705 NNP denotes TBI
T9153 17699-17701 TO denotes to
T9152 17687-17698 JJ denotes susceptible
T9151 17682-17686 JJR denotes more
T9150 17675-17681 VBP denotes appear
T9149 17670-17674 NNS denotes mice
T9148 17655-17669 JJ denotes Nrf2-deficient
T9147 17653-17654 , denotes ,
T9146 17649-17653 NN denotes hand
T9145 17643-17648 JJ denotes other
T9144 17639-17642 DT denotes the
T9143 17636-17638 IN denotes On
T9142 17634-17635 . denotes .
T9141 17633-17634 NNP denotes ]
T9140 17631-17633 CD denotes 19
T9139 17630-17631 NNP denotes [
T9138 17626-17629 NNP denotes TBI
T9137 17620-17625 IN denotes after
T9136 17608-17619 NN denotes hippocampus
T9135 17605-17607 IN denotes of
T9134 17596-17604 NN denotes subfield
T9133 17592-17595 NNP denotes CA3
T9132 17588-17591 DT denotes the
T9131 17584-17587 CC denotes and
T9130 17577-17583 NN denotes cortex
T9129 17573-17576 DT denotes the
T9128 17570-17572 IN denotes in
T9127 17568-17569 , denotes ,
T9126 17556-17568 NN denotes peroxidation
T9125 17550-17555 JJ denotes lipid
T9124 17547-17549 IN denotes of
T9123 17540-17546 NN denotes marker
T9122 17538-17539 DT denotes a
T9121 17536-17537 , denotes ,
T9120 17535-17536 -RRB- denotes )
T9119 17530-17535 JJ denotes 4-HNE
T9118 17529-17530 -LRB- denotes (
T9117 17512-17528 JJ denotes 4-Hydroxynonenal
T9116 17508-17511 IN denotes for
T9115 17491-17507 NN denotes immunoreactivity
T8898 16318-16326 JJ denotes cultured
T8897 16315-16317 IN denotes in
T8896 16309-16314 IN denotes above
T8895 16299-16308 VBN denotes mentioned
T8894 16289-16298 NNS denotes mediators
T8893 16273-16288 JJ denotes proinflammatory
T8892 16269-16272 CC denotes and
T8891 16267-16268 , denotes ,
T8890 16263-16267 NNP denotes MMP9
T8889 16260-16262 IN denotes of
T8888 16245-16259 NN denotes overexpression
T8887 16243-16244 , denotes ,
T8886 16235-16243 NN denotes activity
T8885 16227-16234 JJ denotes binding
T8884 16223-16226 NNP denotes DNA
T8883 16217-16222 NNP denotes NF-κB
T8882 16214-16216 IN denotes of
T8881 16204-16213 NN denotes elevation
T8880 16196-16203 VBD denotes induced
T8879 16189-16195 NN denotes injury
T8878 16181-16188 NN denotes scratch
T8877 16176-16180 IN denotes that
T8876 16167-16175 VBD denotes revealed
T8875 16162-16166 RB denotes also
T8874 16154-16161 NNS denotes results
T8873 16150-16153 PRP$ denotes Our
T8872 16148-16149 . denotes .
T8871 16147-16148 NN denotes ]
T8870 16145-16147 CD denotes 11
T8869 16143-16144 , denotes ,
T8868 16141-16143 CD denotes 10
T8867 16139-16140 , denotes ,
T8866 16138-16139 CD denotes 3
T8865 16137-16138 NNP denotes [
T8864 16133-16136 NNP denotes TBI
T8863 16127-16132 IN denotes after
T6959 15414-15415 . denotes .
T6958 15413-15414 -RRB- denotes )
T6957 15412-15413 CD denotes 6
T6956 15405-15411 NN denotes Figure
T6955 15404-15405 -LRB- denotes (
T6954 15402-15403 -RRB- denotes )
T6953 15398-15402 CD denotes 0.01
T6871 15057-15061 CD denotes 0.01
T6870 15055-15056 VBD denotes <
T6869 15053-15054 NNP denotes P
T6868 15051-15052 , denotes ,
T6867 15046-15051 CD denotes 0.068
T6866 15044-15045 CD denotes ±
T6865 15039-15043 CD denotes 1.01
T6864 15032-15038 IN denotes versus
T6863 15026-15031 CD denotes 0.090
T6862 15024-15025 CD denotes ±
T6861 15019-15023 CD denotes 1.36
T6860 15018-15019 -LRB- denotes (
T6859 15014-15017 NNP denotes TBI
T6858 15011-15013 NNP denotes WT
T6857 15005-15010 NN denotes group
T6856 15002-15004 IN denotes in
T6855 14997-15001 IN denotes than
T6854 14993-14996 NNP denotes TBI
T6853 14990-14992 NNP denotes KO
T6852 14984-14989 NN denotes group
T6851 14981-14983 IN denotes in
T6850 14974-14980 JJR denotes higher
T6849 14970-14973 VBD denotes was
T6848 14965-14969 NNP denotes MMP9
T6847 14962-14964 IN denotes of
T6846 14956-14961 NN denotes level
T6845 14951-14955 NNP denotes mRNA
T6844 14947-14950 DT denotes The
T6843 14945-14946 . denotes .
T6842 14933-14945 NNS denotes counterparts
T6841 14928-14932 NN denotes sham
T6840 14922-14927 PRP$ denotes their
T6839 14917-14921 IN denotes with
T6838 14908-14916 VBN denotes compared
T6837 14905-14907 IN denotes as
T6836 14894-14904 NNS denotes astrocytes
T6835 14891-14893 NNP denotes KO
T6834 14887-14890 CC denotes and
T6833 14884-14886 NNP denotes WT
T6832 14881-14883 IN denotes in
T6831 14874-14880 NN denotes injury
T6830 14866-14873 NN denotes scratch
T6829 14860-14865 IN denotes after
T6828 14851-14859 VBN denotes elevated
T6827 14847-14850 VBD denotes was
T6826 14842-14846 NNP denotes MMP9
T6825 14839-14841 IN denotes of
T6824 14828-14838 NN denotes Expression
T6823 14826-14827 . denotes .
T6822 14816-14826 NN denotes zymography
T6821 14807-14815 JJ denotes gelatine
T6820 14803-14806 CC denotes and
T6819 14801-14802 , denotes ,
T6818 14797-14801 NN denotes blot
T6817 14789-14796 JJ denotes western
T6816 14787-14788 , denotes ,
T6815 14781-14787 NNP denotes RT-PCR
T6814 14778-14780 IN denotes by
T6813 14771-14777 NN denotes injury
T6812 14763-14770 NN denotes scratch
T6811 14757-14762 IN denotes after
T6810 14755-14756 NN denotes h
T6809 14752-14754 CD denotes 24
T6808 14749-14751 IN denotes at
T6807 14738-14748 NNS denotes astrocytes
T6806 14735-14737 IN denotes in
T6805 14730-14734 NNP denotes MMP9
T6804 14727-14729 IN denotes of
T6803 14718-14726 NN denotes activity
T6802 14714-14717 CC denotes and
T6801 14712-14713 , denotes ,
T6800 14706-14712 NNS denotes levels
T6799 14698-14705 NN denotes protein
T6798 14696-14697 , denotes ,
T6797 14692-14696 NNP denotes mRNA
T6796 14688-14691 DT denotes the
T6795 14679-14687 VBD denotes elevated
T6794 14676-14678 PRP denotes we
T6793 14674-14675 , denotes ,
T6792 14669-14674 NN denotes study
T6791 14661-14668 JJ denotes present
T6790 14657-14660 DT denotes the
T6789 14654-14656 IN denotes In
T6788 14652-14653 . denotes .
T6787 14645-14652 NN denotes process
T6786 14632-14644 NN denotes inflammation
T6785 14628-14631 DT denotes the
T6784 14618-14627 VB denotes aggravate
T6783 14615-14617 CC denotes or
T6782 14608-14614 VB denotes induce
T6781 14605-14607 TO denotes to
T6780 14594-14604 NN denotes gelatinase
T6779 14584-14593 JJ denotes important
T6778 14581-14583 DT denotes an
T6777 14578-14580 VBZ denotes is
T6776 14573-14577 NNP denotes MMP9
T6775 14566-14572 NNP denotes Injury
T6774 14558-14565 NNP denotes Scratch
T6773 14552-14557 IN denotes after
T6772 14541-14551 NNP denotes Astrocytes
T6771 14532-14540 NNP denotes Knockout
T6770 14527-14531 NNP denotes Nrf2
T6769 14524-14526 IN denotes in
T6768 14515-14523 VBN denotes Enhanced
T6767 14507-14514 RB denotes Greatly
T6766 14502-14506 VBD denotes Were
T6765 14497-14501 NNP denotes MMP9
T6764 14494-14496 IN denotes of
T6763 14485-14493 NN denotes Activity
T6762 14481-14484 CC denotes and
T6761 14470-14480 NN denotes Expression
T6760 14468-14469 . denotes .
T6759 14465-14468 CD denotes 3.4
T6298 14400-14405 IN denotes while
T6297 14396-14399 NNP denotes TBI
T6296 14393-14395 NNP denotes KO
T6295 14387-14392 NN denotes group
T6294 14383-14386 IN denotes for
T6293 14377-14382 CD denotes 6.339
T6292 14375-14376 CD denotes ±
T6291 14368-14374 CD denotes 190.75
T6290 14364-14367 VBD denotes was
T6289 14361-14363 PRP denotes it
T6288 14359-14360 , denotes ,
T6287 14354-14359 NN denotes IL-1β
T6286 14350-14353 IN denotes For
T6285 14348-14349 . denotes .
T6284 14347-14348 -RRB- denotes )
T6283 14346-14347 -RRB- denotes )
T6282 14345-14346 NN denotes b
T6281 14344-14345 -LRB- denotes (
T6280 14343-14344 CD denotes 4
T6279 14336-14342 NN denotes Figure
T6278 14335-14336 -LRB- denotes (
T6277 14333-14334 -RRB- denotes )
T6276 14329-14333 CD denotes 0.01
T6275 14327-14328 CD denotes <
T6274 14325-14326 NNP denotes P
T6273 14324-14325 -LRB- denotes (
T6272 14320-14323 NNP denotes TBI
T6271 14317-14319 NNP denotes WT
T6270 14311-14316 NN denotes group
T6269 14307-14310 IN denotes for
T6268 14301-14306 CD denotes 0.536
T6267 14299-14300 CD denotes ±
T6266 14293-14298 CD denotes 25.76
T6265 14287-14292 IN denotes while
T6264 14283-14286 NNP denotes TBI
T6263 14280-14282 NNP denotes KO
T6262 14274-14279 NN denotes group
T6261 14270-14273 IN denotes for
T6260 14264-14269 CD denotes 0.786
T6259 14262-14263 CD denotes ±
T6258 14256-14261 CD denotes 36.07
T6257 14252-14255 VBD denotes was
T6256 14249-14251 PRP denotes it
T6255 14247-14248 , denotes ,
T6254 14243-14247 NN denotes IL-6
T6253 14239-14242 IN denotes For
T6252 14237-14238 . denotes .
T6251 14232-14237 NNP denotes IL-1β
T6250 14228-14231 CC denotes and
T6249 14223-14227 NNP denotes IL-6
T6172 13879-13880 CD denotes 3
T6171 13877-13878 -RRB- denotes )
T6170 13876-13877 DT denotes a
T6169 13875-13876 -LRB- denotes (
T6168 13874-13875 CD denotes 3
T6167 13866-13873 NNS denotes Figures
T6166 13865-13866 -LRB- denotes (
T6165 13863-13864 -RRB- denotes )
T6164 13859-13863 CD denotes 0.01
T6163 13857-13858 NNP denotes <
T6162 13855-13856 NNP denotes P
T6161 13853-13854 . denotes .
T6160 13849-13853 NN denotes IL-6
T6159 13845-13848 IN denotes for
T6158 13839-13844 CD denotes 0.073
T6157 13837-13838 CD denotes ±
T6156 13832-13836 CD denotes 0.99
T6155 13825-13831 IN denotes versus
T6154 13819-13824 CD denotes 0.102
T6153 13817-13818 CD denotes ±
T6152 13812-13816 CD denotes 1.28
T6151 13808-13811 CC denotes and
T6150 13806-13807 , denotes ,
T6149 13801-13806 NN denotes IL-1β
T6148 13797-13800 IN denotes for
T6147 13791-13796 CD denotes 0.088
T6146 13789-13790 CD denotes ±
T6145 13784-13788 CD denotes 0.73
T6144 13777-13783 IN denotes versus
T6143 13771-13776 CD denotes 0.064
T6142 13769-13770 CD denotes ±
T6141 13764-13768 CD denotes 1.34
T6140 13762-13763 , denotes ,
T6139 13757-13762 NNP denotes TNF-α
T6138 13753-13756 IN denotes for
T6137 13747-13752 CD denotes 0.110
T6136 13745-13746 CD denotes ±
T6135 13740-13744 CD denotes 1.39
T6134 13733-13739 IN denotes versus
T6133 13727-13732 CD denotes 0.085
T6132 13725-13726 CD denotes ±
T6131 13720-13724 CD denotes 1.66
T6130 13719-13720 -LRB- denotes (
T6129 13715-13718 NNP denotes TBI
T6128 13712-13714 NNP denotes WT
T6127 13706-13711 NN denotes group
T6126 13703-13705 IN denotes in
T6125 13697-13702 DT denotes those
T6124 13692-13696 IN denotes than
T6123 13685-13691 JJR denotes higher
T6122 13680-13684 RB denotes much
T6121 13675-13679 VBD denotes were
T6120 13671-13674 NNP denotes TBI
T6119 13668-13670 NNP denotes KO
T6118 13662-13667 NN denotes group
T6117 13659-13661 IN denotes in
T6116 13649-13658 NNS denotes cytokines
T6115 13643-13648 DT denotes these
T6114 13640-13642 IN denotes of
T6113 13633-13639 NNS denotes levels
T6112 13628-13632 NNP denotes mRNA
T6111 13624-13627 DT denotes the
T6110 13622-13623 , denotes ,
T6109 13614-13622 RB denotes Moreover
T6108 13612-13613 . denotes .
T6107 13600-13612 RB denotes respectively
T6106 13598-13599 , denotes ,
T6105 13586-13598 NNS denotes counterparts
T6104 13581-13585 NN denotes sham
T6103 13575-13580 PRP$ denotes their
T6102 13570-13574 IN denotes with
T6101 13561-13569 VBN denotes compared
T6100 13556-13560 WRB denotes when
T6099 13545-13555 NNS denotes astrocytes
T6098 13542-13544 NNP denotes KO
T6097 13538-13541 CC denotes and
T6096 13535-13537 NNP denotes WT
T6095 13530-13534 DT denotes both
T6094 13527-13529 IN denotes in
T6093 13519-13526 NN denotes scratch
T6092 13513-13518 IN denotes after
T6091 13503-13512 VBN denotes increased
T6090 13498-13502 VBD denotes were
T6089 13493-13497 NNP denotes IL-6
T6088 13489-13492 CC denotes and
T6087 13487-13488 , denotes ,
T6086 13482-13487 NNP denotes IL-1β
T6085 13480-13481 , denotes ,
T6084 13475-13480 NNP denotes TNF-α
T6083 13472-13474 IN denotes of
T6082 13465-13471 NNS denotes levels
T6081 13460-13464 NNP denotes mRNA
T6080 13456-13459 DT denotes the
T6079 13451-13455 IN denotes that
T6078 13445-13450 VBN denotes shown
T6077 13441-13444 VBD denotes was
T6076 13438-13440 PRP denotes It
T6075 13436-13437 . denotes .
T6074 13431-13436 NNP denotes ELISA
T6073 13427-13430 CC denotes and
T6072 13420-13426 NNP denotes RT-PCR
T6071 13417-13419 IN denotes by
T6070 13410-13416 NN denotes injury
T6069 13402-13409 NN denotes scratch
T6068 13396-13401 IN denotes after
T6067 13394-13395 NN denotes h
T6066 13391-13393 CD denotes 24
T6065 13388-13390 IN denotes at
T6064 13377-13387 NNS denotes astrocytes
T6063 13368-13376 JJ denotes cultured
T6062 13365-13367 IN denotes in
T6061 13360-13364 NNP denotes IL-6
T6060 13356-13359 CC denotes and
T6059 13354-13355 , denotes ,
T6058 13349-13354 NNP denotes IL-1β
T6057 13347-13348 , denotes ,
T6056 13342-13347 NNP denotes TNF-α
T6055 13339-13341 IN denotes of
T6054 13332-13338 NNS denotes levels
T6053 13328-13331 DT denotes the
T6052 13321-13327 VBD denotes tested
T6051 13318-13320 PRP denotes we
T6050 13316-13317 , denotes ,
T6049 13312-13316 RB denotes Here
T6048 13310-13311 . denotes .
T6047 13307-13310 NNP denotes TBI
T6046 13301-13306 IN denotes after
T6045 13290-13300 NNS denotes astrocytes
T6044 13287-13289 IN denotes in
T6043 13282-13286 NNS denotes Nrf2
T6042 13278-13281 CC denotes and
T6041 13268-13277 NNS denotes cytokines
T6040 13262-13267 DT denotes these
T6039 13254-13261 IN denotes between
T6038 13241-13253 NN denotes relationship
T6037 13237-13240 DT denotes the
T6036 13231-13236 IN denotes about
T6035 13221-13230 JJ denotes ambiguous
T6034 13215-13220 RB denotes still
T6033 13212-13214 VBZ denotes is
T6032 13209-13211 PRP denotes it
T6031 13205-13208 CC denotes But
T6030 13203-13204 . denotes .
T6029 13202-13203 NNP denotes ]
T6028 13201-13202 CD denotes 5
T6027 13200-13201 NNP denotes [
T6026 13196-13199 NNP denotes TBI
T6025 13190-13195 IN denotes after
T6024 13185-13189 NNS denotes mice
T6023 13170-13184 JJ denotes Nrf2-deficient
T6022 13167-13169 IN denotes of
T6021 13161-13166 NN denotes brain
T6020 13157-13160 DT denotes the
T6019 13154-13156 IN denotes in
T6018 13145-13153 VBN denotes observed
T6017 13140-13144 VBD denotes were
T6016 13130-13139 NNS denotes cytokines
T6015 13114-13129 JJ denotes proinflammatory
T6014 13109-13113 JJ denotes such
T6013 13106-13108 IN denotes of
T6012 13099-13105 NNS denotes levels
T6011 13092-13098 JJR denotes higher
T6010 13087-13091 IN denotes that
T6009 13080-13086 VBN denotes proved
T6008 13076-13079 VBZ denotes has
T6007 13070-13075 NN denotes study
T6006 13061-13069 JJ denotes previous
T6005 13057-13060 PRP$ denotes our
T6004 13053-13056 CC denotes And
T6003 13051-13052 . denotes .
T6002 13050-13051 NN denotes ]
T6001 13049-13050 CD denotes 8
T6000 13047-13048 , denotes ,
T5999 13046-13047 CD denotes 2
T5998 13045-13046 VBP denotes [
T5997 13037-13044 NNS denotes animals
T5996 13034-13036 IN denotes in
T5995 13030-13033 NNP denotes TBI
T5994 13024-13029 IN denotes after
T5993 13014-13023 VBN denotes increased
T5992 13010-13013 VBP denotes are
T5991 13000-13009 NNS denotes cytokines
T5990 12984-12999 JJ denotes proinflammatory
T5989 12978-12983 DT denotes these
T5988 12973-12977 IN denotes that
T5987 12964-12972 VBN denotes revealed
T5986 12959-12963 VBN denotes been
T5985 12955-12958 VBZ denotes has
T5984 12952-12954 PRP denotes It
T5983 12950-12951 . denotes .
T5982 12945-12950 NN denotes NF-κB
T5981 12942-12944 IN denotes of
T5980 12933-12941 NN denotes activity
T5979 12922-12932 JJ denotes endogenous
T5978 12918-12921 DT denotes the
T5977 12910-12917 VBP denotes reflect
T5976 12906-12909 CC denotes and
T5975 12900-12905 NNP denotes NF-κB
T5974 12897-12899 IN denotes by
T5973 12887-12896 VBN denotes regulated
T5972 12883-12886 VBP denotes are
T5971 12878-12882 NNP denotes IL-6
T5970 12874-12877 CC denotes and
T5969 12872-12873 , denotes ,
T5968 12867-12872 NNP denotes IL-1β
T5967 12865-12866 , denotes ,
T5966 12860-12865 NNP denotes TNF-α
T5965 12858-12859 , denotes ,
T5964 12854-12858 VBP denotes know
T5963 12850-12853 DT denotes all
T5962 12847-12849 PRP denotes we
T5961 12844-12846 IN denotes As
T5960 12837-12843 NNP denotes Injury
T5959 12829-12836 NNP denotes Scratch
T5958 12823-12828 IN denotes after
T5957 12812-12822 NNP denotes Astrocytes
T6248 14220-14222 IN denotes of
T6247 14214-14219 NN denotes level
T6246 14206-14213 NN denotes protein
T6245 14203-14205 IN denotes in
T6244 14194-14202 VBN denotes observed
T6243 14190-14193 VBD denotes was
T6242 14181-14189 NN denotes tendency
T6241 14173-14180 JJ denotes Similar
T6240 14171-14172 . denotes .
T6239 14170-14171 -RRB- denotes )
T6238 14169-14170 -RRB- denotes )
T6237 14168-14169 DT denotes a
T6236 14167-14168 -LRB- denotes (
T6235 14166-14167 CD denotes 4
T6234 14159-14165 NN denotes Figure
T6233 14158-14159 -LRB- denotes (
T6232 14156-14157 -RRB- denotes )
T6231 14152-14156 CD denotes 0.01
T6230 14150-14151 VBD denotes <
T6229 14148-14149 NNP denotes P
T6228 14146-14147 , denotes ,
T6227 14141-14146 CD denotes 0.087
T6226 14139-14140 CD denotes ±
T6225 14134-14138 CD denotes 2.31
T6224 14127-14133 IN denotes versus
T6223 14121-14126 CD denotes 0.156
T6222 14119-14120 CD denotes ±
T6221 14114-14118 CD denotes 3.67
T6220 14113-14114 -LRB- denotes (
T6219 14109-14112 NNP denotes TBI
T6218 14106-14108 NNP denotes WT
T6217 14100-14105 NN denotes group
T6216 14097-14099 IN denotes in
T6215 14092-14096 IN denotes than
T6214 14088-14091 NNP denotes TBI
T6213 14085-14087 NNP denotes KO
T6212 14079-14084 NN denotes group
T6211 14076-14078 IN denotes in
T6210 14069-14075 JJR denotes higher
T6209 14064-14068 RB denotes also
T6208 14060-14063 VBD denotes was
T6207 14057-14059 PRP denotes it
T6206 14053-14056 CC denotes And
T6205 14051-14052 . denotes .
T6204 14039-14051 NNS denotes counterparts
T6203 14034-14038 NN denotes sham
T6202 14028-14033 PRP$ denotes their
T6201 14023-14027 IN denotes with
T6200 14014-14022 VBN denotes compared
T6199 14009-14013 WRB denotes when
T6198 13998-14008 NNS denotes astrocytes
T6197 13995-13997 NNP denotes KO
T6196 13991-13994 CC denotes and
T6195 13988-13990 NNP denotes WT
T6194 13985-13987 IN denotes in
T6193 13978-13984 NN denotes injury
T6192 13970-13977 NN denotes scratch
T6191 13964-13969 IN denotes after
T6190 13952-13963 VBN denotes upregulated
T6189 13948-13951 VBD denotes was
T6188 13942-13947 NNP denotes TNF-α
T6187 13939-13941 IN denotes of
T6186 13933-13938 NN denotes level
T6185 13925-13932 NN denotes protein
T6184 13921-13924 DT denotes the
T6183 13916-13920 IN denotes that
T6182 13907-13915 VBD denotes revealed
T6181 13901-13906 NNP denotes ELISA
T6180 13898-13900 IN denotes of
T6179 13890-13897 NNS denotes results
T6178 13886-13889 DT denotes The
T6177 13884-13885 . denotes .
T6176 13883-13884 -RRB- denotes )
T6175 13882-13883 -RRB- denotes )
T6174 13881-13882 LS denotes d
T6173 13880-13881 -LRB- denotes (
T5956 12803-12811 NNP denotes Knockout
T5955 12798-12802 NNP denotes Nrf2
T5954 12795-12797 IN denotes in
T5953 12786-12794 NNP denotes Elevated
T5952 12782-12785 VBD denotes Was
T5951 12772-12781 NNP denotes Cytokines
T5950 12756-12771 NNP denotes Proinflammatory
T5949 12753-12755 IN denotes of
T5948 12742-12752 NN denotes Expression
T5947 12740-12741 . denotes .
T5946 12737-12740 CD denotes 3.3
T5327 12734-12735 . denotes .
T5326 12733-12734 -RRB- denotes )
T5325 12732-12733 CD denotes 2
T5324 12725-12731 NN denotes Figure
T5323 12724-12725 -LRB- denotes (
T5322 12722-12723 -RRB- denotes )
T5321 12718-12722 CD denotes 0.01
T5320 12716-12717 VBD denotes <
T5319 12714-12715 NNP denotes P
T5318 12712-12713 , denotes ,
T5317 12707-12712 CD denotes 0.072
T5316 12705-12706 CD denotes ±
T5315 12700-12704 CD denotes 2.28
T5314 12693-12699 IN denotes versus
T5313 12687-12692 CD denotes 0.173
T5312 12685-12686 CD denotes ±
T5311 12680-12684 CD denotes 2.67
T5310 12679-12680 -LRB- denotes (
T5309 12675-12678 NNP denotes TBI
T5308 12672-12674 NNP denotes WT
T5307 12666-12671 NN denotes group
T5306 12663-12665 IN denotes in
T5305 12658-12662 IN denotes than
T5304 12654-12657 NNP denotes TBI
T5303 12651-12653 NNP denotes KO
T5302 12645-12650 NN denotes group
T5301 12642-12644 IN denotes in
T5300 12633-12641 VBN denotes observed
T5299 12629-12632 VBD denotes was
T5298 12620-12628 NN denotes activity
T5297 12614-12619 NN denotes NF-κB
T5296 12607-12613 JJR denotes higher
T5295 12601-12606 IN denotes while
T5294 12599-12600 , denotes ,
T5293 12590-12599 NNS denotes genotypes
T5292 12585-12589 DT denotes both
T5291 12582-12584 IN denotes of
T5290 12571-12581 NNS denotes astrocytes
T5289 12568-12570 IN denotes in
T5288 12562-12567 NN denotes NF-κB
T5287 12559-12561 IN denotes of
T5286 12548-12558 NN denotes activation
T5285 12540-12547 VBD denotes induced
T5284 12533-12539 NN denotes injury
T5283 12525-12532 NN denotes Scratch
T5282 12523-12524 . denotes .
T5281 12517-12523 NN denotes injury
T5280 12509-12516 NN denotes scratch
T5279 12503-12508 IN denotes after
T5278 12501-12502 NN denotes h
T5087 12229-12230 . denotes .
T5086 12223-12229 NNS denotes groups
T5085 12209-12222 JJ denotes sham-operated
T5084 12205-12208 CD denotes two
T5083 12197-12204 IN denotes between
T5082 12186-12196 NN denotes difference
T5081 12183-12185 DT denotes no
T5080 12179-12182 VBD denotes was
T5079 12173-12178 EX denotes There
T5078 12171-12172 . denotes .
T5077 12170-12171 -RRB- denotes )
T5076 12169-12170 CD denotes 1
T5075 12162-12168 NNP denotes Figure
T5074 12161-12162 -LRB- denotes (
T5073 12157-12160 NNP denotes TBI
T5072 12154-12156 NNP denotes KO
T5071 12148-12153 NN denotes group
T5070 12145-12147 IN denotes in
T5069 12139-12144 NN denotes death
T5068 12134-12138 NN denotes cell
T5067 12129-12133 JJR denotes more
T5066 12119-12128 VBD denotes suggested
T5065 12114-12118 RB denotes also
T5062 12108-12113 WDT denotes which
T5025 11971-11976 NN denotes group
T5024 11968-11970 IN denotes in
T5023 11963-11967 NN denotes line
T5022 11955-11962 NN denotes scratch
T5021 11952-11954 IN denotes of
T5020 11946-11951 NNS denotes sides
T5019 11941-11945 DT denotes both
T5018 11938-11940 IN denotes in
T5017 11929-11937 VBN denotes observed
T5016 11922-11928 RB denotes mostly
T5015 11917-11921 VBD denotes were
T5014 11906-11916 NN denotes distortion
T5013 11898-11905 NN denotes process
T5012 11886-11897 JJ denotes cytoplasmic
T5011 11882-11885 CC denotes and
T5010 11880-11881 , denotes ,
T5009 11876-11880 VBD denotes lost
T5008 11871-11875 NN denotes cell
T5007 11869-11870 , denotes ,
T5006 11864-11869 NN denotes plate
T5005 11856-11863 NN denotes culture
T5004 11851-11855 IN denotes from
T5003 11840-11850 NN denotes detachment
T5002 11836-11839 DT denotes The
T5001 11834-11835 . denotes .
T5000 11827-11834 NN denotes scratch
T4999 11821-11826 IN denotes after
T4998 11819-11820 NN denotes h
T4997 11816-11818 CD denotes 24
T4839 11657-11658 . denotes .
T4838 11649-11657 NN denotes software
T4837 11644-11648 CD denotes 18.0
T4836 11639-11643 NNP denotes SPSS
T4835 11633-11638 VBG denotes using
T4834 11630-11632 IN denotes by
T4833 11620-11629 VBN denotes performed
T4832 11615-11619 VBD denotes were
T4831 11606-11614 NNS denotes analyses
T4830 11602-11605 DT denotes All
T4829 11600-11601 . denotes .
T4828 11589-11600 JJ denotes significant
T4827 11586-11588 VB denotes be
T4826 11583-11585 TO denotes to
T4825 11572-11582 VBN denotes considered
T4824 11567-11571 VBD denotes were
T4823 11562-11566 CD denotes 0.05
T4822 11561-11562 VBP denotes <
T4821 11554-11560 NNS denotes values
T4820 11552-11553 NN denotes P
T4819 11550-11551 . denotes .
T4818 11546-11550 NN denotes test
T4817 11535-11545 NN denotes comparison
T4816 11526-11534 JJ denotes multiple
T4815 11522-11525 NNP denotes LSD
T4814 11518-11521 CC denotes and
T4813 11512-11517 NNP denotes ANOVA
T4812 11509-11511 IN denotes by
T4811 11499-11508 VBN denotes evaluated
T4810 11495-11498 CC denotes and
T4809 11492-11494 NNP denotes SD
T4808 11490-11491 NN denotes ±
T4807 11485-11489 JJ denotes mean
T4806 11482-11484 IN denotes as
T4805 11472-11481 VBN denotes expressed
T4804 11467-11471 VBD denotes were
T4803 11462-11466 NNP denotes Data
T4802 11453-11461 NNP denotes Analysis
T4801 11441-11452 NNP denotes Statistical
T4800 11439-11440 . denotes .
T4799 11436-11439 CD denotes 2.9
T5277 12498-12500 CD denotes 24
T5276 12495-12497 IN denotes at
T5275 12490-12494 NNP denotes EMSA
T5274 12487-12489 IN denotes by
T5273 12482-12486 NNS denotes mice
T5272 12479-12481 NNP denotes KO
T5271 12476-12478 CC denotes or
T5270 12473-12475 NNP denotes WT
T5269 12468-12472 NNP denotes Nrf2
T5268 12463-12467 IN denotes from
T5267 12452-12462 NNS denotes astrocytes
T5266 12449-12451 IN denotes of
T5265 12443-12448 NN denotes NF-κB
T5264 12440-12442 IN denotes of
T5263 12431-12439 NN denotes activity
T5262 12419-12430 JJ denotes DNA-binding
T5261 12415-12418 DT denotes the
T5260 12407-12414 VBD denotes studied
T5259 12404-12406 PRP denotes we
T5258 12402-12403 , denotes ,
T5257 12398-12402 RB denotes Here
T5256 12396-12397 . denotes .
T5255 12395-12396 NNP denotes ]
T5254 12394-12395 CD denotes 2
T5253 12393-12394 NNP denotes [
T5252 12389-12392 NNP denotes TBI
T5251 12383-12388 IN denotes after
T5250 12377-12382 NN denotes brain
T5249 12374-12376 IN denotes in
T5248 12364-12373 VBN denotes activated
T5247 12361-12363 VBZ denotes is
T5246 12355-12360 NNP denotes NF-κB
T5245 12350-12354 IN denotes that
T5244 12341-12349 VBN denotes reported
T5243 12336-12340 VBN denotes been
T5242 12332-12335 VBZ denotes has
T5241 12329-12331 PRP denotes It
T5240 12322-12328 NNP denotes Injury
T5239 12314-12321 NNP denotes Scratch
T5238 12308-12313 IN denotes after
T5237 12299-12307 NN denotes Activity
T4729 11433-11434 . denotes .
T4728 11427-11433 NNP denotes ImageJ
T4727 11419-11426 NN denotes program
T4726 11410-11418 NN denotes analysis
T4725 11404-11409 NN denotes image
T4723 11389-11398 VBN denotes performed
T4722 11385-11388 VBD denotes was
T4721 11377-11384 NN denotes density
T4720 11372-11376 NN denotes band
T4719 11367-11371 NNP denotes MMP9
T4718 11364-11366 IN denotes of
T4717 11349-11363 NN denotes Quantification
T4716 11347-11348 . denotes .
T4715 11338-11347 NN denotes substrate
T4714 11334-11337 DT denotes the
T4713 11331-11333 IN denotes of
T4712 11325-11330 NN denotes lysis
T4711 11321-11324 DT denotes the
T4710 11311-11320 VBZ denotes indicates
T4709 11305-11310 WDT denotes which
T4708 11301-11304 NN denotes gel
T4707 11296-11300 JJ denotes blue
T4706 11293-11295 IN denotes in
T4705 11287-11292 NNS denotes bands
T4704 11281-11286 JJ denotes clear
T4703 11278-11280 IN denotes by
T4702 11271-11277 VBN denotes showed
T4701 11266-11270 VBD denotes were
T4700 11255-11265 NNS denotes activities
T4699 11243-11254 JJ denotes Proteolytic
T4698 11241-11242 . denotes .
T4697 11233-11241 NN denotes methanol
T4696 11231-11232 NN denotes %
T4695 11229-11231 CD denotes 50
T4694 11226-11228 IN denotes in
T4693 11216-11225 VBN denotes destained
T4692 11212-11215 CC denotes and
T4686 11184-11188 IN denotes with
T4685 11176-11183 VBN denotes stained
T4684 11172-11175 VBD denotes was
T4683 11168-11171 NN denotes gel
T4682 11163-11167 DT denotes each
T4652 11026-11027 NN denotes %
T4651 11023-11026 CD denotes 2.5
T4650 11020-11022 IN denotes in
T4649 11009-11019 NN denotes incubation
T4648 11006-11008 IN denotes by
T4647 10996-11005 VBN denotes renatured
T4646 10991-10995 VBD denotes were
T4645 10982-10990 NNS denotes proteins
T4644 10978-10981 DT denotes the
T4643 10973-10977 RB denotes Then
T4642 10971-10972 . denotes .
T4641 10956-10971 NN denotes electrophoresis
T4640 10953-10955 IN denotes by
T4639 10943-10952 VBN denotes separated
T4638 10938-10942 VBD denotes were
T4637 10929-10937 NNS denotes extracts
T4636 10921-10928 NN denotes protein
T4635 10911-10920 JJ denotes cytosolic
T4634 10908-10910 NN denotes μg
T4633 10905-10907 CD denotes 40
T4632 10903-10904 , denotes ,
T4631 10896-10903 RB denotes Briefly
T4630 10894-10895 . denotes .
T4629 10893-10894 -RRB- denotes )
T4628 10890-10893 NNP denotes USA
T4627 10888-10889 , denotes ,
T4626 10886-10888 NNP denotes MA
T4625 10884-10885 , denotes ,
T4624 10881-10884 NNP denotes Inc
T4623 10869-10880 NNP denotes Scientifics
T4622 10862-10868 NNP denotes Genmed
T4621 10861-10862 -LRB- denotes (
T4620 10848-10860 NNS denotes instructions
T4619 10845-10847 POS denotes 's
T4618 10833-10845 NN denotes manufacturer
T4617 10829-10832 DT denotes the
T4616 10826-10828 TO denotes to
T4615 10816-10825 VBG denotes according
T4614 10806-10815 VBN denotes performed
T4613 10802-10805 VBD denotes was
T4612 10791-10801 NN denotes zymography
T4611 10783-10790 NNP denotes Gelatin
T4610 10780-10782 NNP denotes C.
T4609 10779-10780 NN denotes °
T4608 10777-10779 CD denotes 20
T4607 10773-10775 IN denotes at
T4606 10766-10772 VBD denotes stored
T4605 10762-10765 CC denotes and
T4604 10747-10761 NN denotes centrifugation
T4603 10744-10746 IN denotes by
T4602 10734-10743 VBN denotes separated
T4601 10729-10733 VBD denotes were
T4600 10720-10728 NNS denotes extracts
T4599 10712-10719 JJ denotes Soluble
T4598 10710-10711 . denotes .
T4597 10704-10710 NNP denotes Triton
T4596 10702-10703 NN denotes %
T4595 10698-10702 CD denotes 0.01
T4594 10694-10697 CC denotes and
T4593 10692-10693 , denotes ,
T4592 10688-10692 NNP denotes NaN3
T4591 10685-10687 NNP denotes mM
T4590 10681-10684 CD denotes 0.2
T4589 10679-10680 , denotes ,
T4588 10674-10679 NNP denotes CaCl2
T4587 10671-10673 NNP denotes mM
T4586 10669-10670 CD denotes 5
T4585 10667-10668 , denotes ,
T4584 10663-10667 NNP denotes NaCl
T4583 10660-10662 NNP denotes mM
T4582 10656-10659 CD denotes 150
T4581 10654-10655 , denotes ,
T4580 10653-10654 -RRB- denotes )
T4579 10650-10653 CD denotes 7.4
T4578 10647-10649 NNP denotes pH
T4577 10646-10647 -LRB- denotes (
T4576 10637-10645 NNP denotes Tris-HCl
T4575 10634-10636 NNP denotes mM
T4574 10631-10633 CD denotes 50
T4573 10620-10630 VBG denotes containing
T4572 10613-10619 NN denotes buffer
T4571 10607-10612 NN denotes lysis
T4570 10604-10606 IN denotes in
T4569 10592-10603 VBN denotes homogenized
T4568 10587-10591 VBD denotes were
T4567 10580-10586 NNS denotes groups
T4566 10575-10579 CD denotes four
T4565 10572-10574 IN denotes of
T4564 10566-10571 NNP denotes Cells
T4563 10555-10565 NNP denotes Zymography
T4562 10546-10554 NNP denotes Gelatine
T4561 10544-10545 . denotes .
T4560 10541-10544 CD denotes 2.8
T3991 9365-9366 . denotes .
T3990 9357-9365 NN denotes standard
T3989 9348-9356 JJ denotes internal
T3988 9345-9347 IN denotes as
T3987 9340-9344 VBN denotes used
T3986 9336-9339 VBD denotes was
T3985 9328-9335 NN denotes β-actin
T3984 9325-9327 IN denotes of
T3983 9319-9324 NN denotes level
T3982 9315-9318 DT denotes The
T3981 9313-9314 . denotes .
T3980 9308-9313 NNS denotes blots
T3979 9304-9307 DT denotes the
T3978 9301-9303 IN denotes of
T3977 9291-9300 NN denotes intensity
T3976 9287-9290 DT denotes the
T3975 9279-9286 VB denotes analyze
T3974 9276-9278 TO denotes to
T3973 9271-9275 VBN denotes used
T3972 9267-9270 VBD denotes was
T3971 9260-9266 NNP denotes ImageJ
T3970 9258-9259 . denotes .
T3969 9257-9258 -RRB- denotes )
T3704 7894-7901 NN denotes protein
T3703 7888-7893 JJ denotes total
T3702 7881-7887 VB denotes obtain
T3701 7878-7880 TO denotes To
T3700 7873-7877 NNP denotes Blot
T3699 7865-7872 JJ denotes Western
T3698 7863-7864 . denotes .
T3697 7860-7863 CD denotes 2.6
T4691 11210-11211 NN denotes h
T4690 11208-11209 CD denotes 1
T4689 11204-11207 IN denotes for
T4688 11199-11203 JJ denotes blue
T4687 11189-11198 NNP denotes Coomassie
T3289 7536-7539 VBD denotes was
T3288 7522-7535 NN denotes transcription
T3287 7514-7521 VB denotes Reverse
T3286 7511-7513 NNP denotes C.
T3285 7510-7511 NN denotes °
T3284 7508-7510 CD denotes 20
T3283 7507-7508 CD denotes −
T3282 7504-7506 IN denotes in
T3281 7497-7503 VBN denotes stored
T3280 7493-7496 VBD denotes was
T3279 7488-7492 NNP denotes cDNA
T3278 7484-7487 DT denotes The
T3277 7482-7483 . denotes .
T3276 7474-7482 NN denotes protocol
T3275 7471-7473 POS denotes 's
T3274 7459-7471 NN denotes manufacturer
T3273 7455-7458 DT denotes the
T3272 7452-7454 TO denotes to
T3271 7442-7451 VBG denotes according
T3270 7440-7441 -RRB- denotes )
T3269 7435-7440 NNP denotes China
T3268 7433-7434 , denotes ,
T3267 7426-7433 NNP denotes Jiangsu
T3266 7424-7425 , denotes ,
T3265 7416-7424 NNP denotes Biunique
T3264 7415-7416 -LRB- denotes (
T3263 7408-7414 NN denotes RT-Kit
T3262 7398-7407 JJ denotes BU-Script
T3261 7393-7397 IN denotes with
T3260 7389-7392 NNP denotes RNA
T3259 7383-7388 JJ denotes total
T3258 7380-7382 IN denotes of
T3257 7377-7379 NN denotes μg
T3256 7375-7376 CD denotes 2
T3255 7370-7374 IN denotes from
T3254 7358-7369 VBN denotes synthesized
T3253 7354-7357 VBD denotes was
T3252 7349-7353 NNP denotes cDNA
T3251 7333-7348 JJ denotes single-stranded
T3250 7329-7332 CC denotes and
T3249 7327-7328 , denotes ,
T3248 7326-7327 -RRB- denotes )
T3247 7323-7326 NNP denotes USA
T3246 7321-7322 , denotes ,
T3245 7319-7321 NNP denotes CA
T3244 7317-7318 , denotes ,
T3243 7307-7317 NNP denotes Invitrogen
T3242 7306-7307 -LRB- denotes (
T3241 7299-7305 NNP denotes Trizol
T3240 7294-7298 IN denotes with
T3239 7285-7293 VBN denotes isolated
T3238 7281-7284 VBD denotes was
T3237 7277-7280 NNP denotes RNA
T3236 7271-7276 NNP denotes Total
T3235 7264-7270 NNP denotes RT-PCR
T3234 7262-7263 . denotes .
T3233 7259-7262 CD denotes 2.5
T3894 8837-8850 RB denotes appropriately
T3893 8829-8836 VBN denotes diluted
T3892 8825-8828 VBD denotes was
T3891 8816-8824 NN denotes antibody
T3865 8702-8705 NN denotes kDa
T3864 8699-8701 CD denotes 43
T3863 8697-8698 , denotes ,
T3862 8694-8697 NNP denotes USA
T3861 8692-8693 , denotes ,
T3860 8690-8692 NNP denotes CA
T3859 8688-8689 , denotes ,
T3858 8675-8688 NNP denotes Biotechnology
T3857 8670-8674 NNP denotes Cruz
T3856 8664-8669 NNP denotes Santa
T3855 8662-8663 , denotes ,
T3854 8653-8662 JJ denotes sc-130657
T3853 8652-8653 -LRB- denotes (
T3852 8639-8651 JJ denotes anti-β-actin
T3851 8637-8638 : denotes :
T3850 8633-8637 VBN denotes used
T3849 8628-8632 VBD denotes were
T3848 8617-8627 NNS denotes antibodies
T3847 8607-8616 VBG denotes following
T3846 8603-8606 DT denotes the
T3845 8599-8602 CC denotes and
T3844 8597-8598 , denotes ,
T3843 8596-8597 NNP denotes C
T3842 8595-8596 CD denotes °
T3841 8594-8595 CD denotes 4
T3840 8591-8593 IN denotes at
T3839 8581-8590 JJ denotes overnight
T3838 8574-8580 NN denotes buffer
T3837 8567-8573 NN denotes saline
T3836 8564-8566 IN denotes in
T3835 8559-8563 NN denotes milk
T3834 8555-8558 JJ denotes dry
T3833 8548-8554 JJ denotes nonfat
T3832 8546-8547 NN denotes %
T3831 8545-8546 CD denotes 5
T3830 8540-8544 IN denotes with
T3829 8532-8539 VBN denotes blocked
T3828 8527-8531 VBD denotes were
T3827 8517-8526 NNS denotes membranes
T3826 8515-8516 , denotes ,
T3825 8501-8515 VBG denotes immunoblotting
T3824 8497-8500 IN denotes For
T3823 8495-8496 . denotes .
T3822 8487-8495 NN denotes membrane
T3821 8472-8486 JJ denotes nitrocellulose
T3820 8470-8471 DT denotes a
T3819 8465-8469 IN denotes onto
T3818 8450-8464 VBD denotes electroblotted
T3817 8446-8449 CC denotes and
T3816 8444-8445 , denotes ,
T3815 8429-8444 NN denotes electrophoresis
T3814 8425-8428 NN denotes gel
T3813 8410-8424 NN denotes polyacrylamide
T3812 8402-8409 NN denotes sulfate
T3811 8394-8401 NN denotes dodecyl
T3810 8387-8393 NN denotes sodium
T3809 8385-8386 NN denotes %
T3808 8383-8385 CD denotes 10
T3787 8249-8252 NNP denotes USA
T3786 8247-8248 , denotes ,
T3785 8245-8247 NNP denotes IL
T3784 8243-8244 , denotes ,
T3783 8237-8243 NNP denotes Pierce
T3782 8236-8237 -LRB- denotes (
T3781 8228-8235 NNP denotes Reagent
T3780 8222-8227 NNP denotes Assay
T3779 8214-8221 NNP denotes Protein
T3778 8209-8213 NNP denotes Plus
T3777 8199-8208 NNP denotes Coomassie
T3776 8196-8198 IN denotes by
T3775 8186-8195 VBN denotes estimated
T3774 8181-8185 VBD denotes were
T3773 8166-8180 NNS denotes concentrations
T3772 8158-8165 NNP denotes Protein
T3771 8155-8157 NNP denotes C.
T3770 8154-8155 CD denotes °
T3769 8153-8154 CD denotes 4
T3768 8150-8152 IN denotes at
T3767 8146-8149 NN denotes min
T3766 8143-8145 CD denotes 15
T3765 8139-8142 IN denotes for
T3764 8137-8138 NN denotes g
T3763 8130-8136 CD denotes 12,000
T3762 8127-8129 IN denotes at
T3761 8115-8126 VBN denotes centrifuged
T3760 8111-8114 CC denotes and
T3759 8109-8110 -RRB- denotes )
T3758 8106-8109 CD denotes 7.4
T3757 8103-8105 NNP denotes pH
T3756 8099-8102 NNP denotes PBS
T3755 8096-8098 IN denotes in
T3754 8087-8095 NN denotes cocktail
T3753 8077-8086 NN denotes inhibitor
T3752 8068-8076 NN denotes protease
T3751 8064-8067 CC denotes and
T3750 8062-8063 , denotes ,
T3749 8058-8062 NNP denotes PMSF
T3748 8055-8057 NNP denotes mM
T3747 8053-8054 CD denotes 1
T3746 8051-8052 , denotes ,
T3745 8048-8051 NNP denotes DTT
T3744 8045-8047 NNP denotes mM
T3743 8041-8044 CD denotes 0.5
T3742 8039-8040 , denotes ,
T3741 8036-8039 NNP denotes NaF
T3740 8033-8035 NNP denotes mM
T3739 8030-8032 CD denotes 20
T3738 8028-8029 , denotes ,
T3737 8022-8028 NNP denotes Na3VO4
T3736 8019-8021 NNP denotes mM
T3735 8017-8018 CD denotes 1
T3734 8015-8016 , denotes ,
T3733 8011-8015 NNP denotes EGTA
T3732 8008-8010 NNP denotes mM
T3731 8006-8007 CD denotes 1
T3730 8004-8005 , denotes ,
T3729 8000-8004 NNP denotes EDTA
T3728 7997-7999 NNP denotes mM
T3727 7995-7996 CD denotes 1
T3726 7993-7994 , denotes ,
T3725 7990-7993 NNP denotes SDS
T3724 7988-7989 NN denotes %
T3723 7985-7988 CD denotes 0.1
T3722 7983-7984 , denotes ,
T3721 7971-7983 NN denotes deoxycholate
T3720 7964-7970 NN denotes sodium
T3719 7962-7963 NN denotes %
T3718 7959-7962 CD denotes 0.5
T3717 7957-7958 , denotes ,
T3716 7953-7957 CD denotes NP40
T3715 7951-7952 NN denotes %
T3714 7950-7951 CD denotes 1
T3713 7949-7950 -LRB- denotes (
T3712 7942-7948 NN denotes buffer
T3711 7937-7941 NNP denotes RIPA
T3710 7934-7936 IN denotes in
T3709 7922-7933 VBN denotes homogenized
T3708 7917-7921 VBD denotes were
T3707 7911-7916 NNS denotes cells
T3706 7909-7910 , denotes ,
T3705 7902-7909 VBZ denotes lysates
T3035 7256-7257 . denotes .
T3034 7250-7256 NNP denotes ImageJ
T3033 7241-7249 NN denotes software
T3032 7238-7240 IN denotes by
T3031 7227-7237 VBN denotes quantified
T3030 7222-7226 VBD denotes were
T3029 7213-7221 NN denotes activity
T3028 7205-7212 JJ denotes binding
T3027 7201-7204 NNP denotes DNA
T3026 7195-7200 NNP denotes NF-κB
T3025 7192-7194 IN denotes of
T3024 7185-7191 NNS denotes Levels
T3023 7183-7184 . denotes .
T3022 7182-7183 -RRB- denotes )
T3021 7177-7182 NNP denotes Japan
T3020 7175-7176 , denotes ,
T3019 7170-7175 NNP denotes Tokyo
T3018 7168-7169 , denotes ,
T3017 7159-7168 NNP denotes Hyperfilm
T3016 7154-7158 NNP denotes Fuji
T3015 7153-7154 -LRB- denotes (
T3014 7148-7152 NN denotes film
T3013 7142-7147 NN denotes X-ray
T3012 7139-7141 TO denotes to
T3011 7131-7138 VBN denotes exposed
T3010 7127-7130 CC denotes and
T3009 7121-7126 VBN denotes dried
T3008 7117-7120 VBD denotes was
T3007 7113-7116 NN denotes gel
T3006 7109-7112 DT denotes the
T3005 7107-7108 , denotes ,
T3004 7092-7107 NN denotes electrophoresis
T3003 7086-7091 IN denotes After
T3002 7084-7085 . denotes .
T3001 7083-7084 NNP denotes C
T3000 7082-7083 CD denotes °
T2999 7081-7082 CD denotes 4
T2998 7078-7080 IN denotes at
T2997 7073-7077 NN denotes hour
T2996 7071-7072 CD denotes 1
T2995 7067-7070 IN denotes for
T2994 7065-7066 NN denotes V
T2993 7061-7064 CD denotes 390
T2992 7058-7060 IN denotes at
T2991 7056-7057 -RRB- denotes )
T2990 7040-7056 NNP denotes tris-borate-EDTA
T2989 7039-7040 -LRB- denotes (
T2988 7032-7038 NN denotes buffer
T2987 7028-7031 NNP denotes TBE
T2986 7026-7027 CD denotes ×
T2985 7022-7025 CD denotes 0.5
T2984 7019-7021 IN denotes in
T2983 7015-7018 NN denotes gel
T2982 7000-7014 NN denotes polyacrylamide
T2981 6986-6999 JJ denotes nondenaturing
T2980 6984-6985 NN denotes %
T2979 6983-6984 CD denotes 4
T2978 6980-6982 IN denotes on
T2977 6964-6979 NN denotes electrophoresis
T2976 6961-6963 IN denotes by
T2975 6951-6960 VBN denotes separated
T2974 6946-6950 VBD denotes were
T2973 6936-6945 NNS denotes complexes
T2972 6924-6935 JJ denotes DNA-protein
T2971 6920-6923 DT denotes The
T2970 6918-6919 . denotes .
T2969 6915-6918 NN denotes ice
T2968 6912-6914 IN denotes on
T2967 6904-6911 NNS denotes minutes
T2966 6901-6903 CD denotes 20
T2965 6897-6900 IN denotes for
T2964 6887-6896 VBN denotes continued
T2963 6883-6886 VBD denotes was
T2962 6872-6882 NN denotes incubation
T2961 6868-6871 DT denotes the
T2960 6866-6867 , denotes ,
T2959 6861-6866 NN denotes probe
T2958 6845-6860 NN denotes oligonucleotide
T2957 6834-6844 JJ denotes 32P-labled
T2956 6831-6833 NNP denotes μL
T2955 6829-6830 CD denotes 1
T2954 6825-6828 DT denotes the
T2953 6822-6824 IN denotes of
T2952 6813-6821 NN denotes addition
T2951 6807-6812 IN denotes After
T2950 6805-6806 . denotes .
T2949 6802-6805 NN denotes ice
T2948 6799-6801 IN denotes on
T2947 6791-6798 NNS denotes minutes
T2946 6788-6790 CD denotes 20
T2945 6784-6787 IN denotes for
T2944 6778-6783 NN denotes dI-dC
T2943 6773-6777 NN denotes poly
T2942 6770-6772 NN denotes μg
T2941 6768-6769 CD denotes 2
T2940 6764-6767 CC denotes and
T2939 6762-6763 , denotes ,
T2938 6759-6762 NNP denotes DTT
T2937 6756-6758 NNP denotes mM
T2936 6752-6755 CD denotes 0.5
T2935 6750-6751 , denotes ,
T2934 6746-6750 NNP denotes EDTA
T2933 6743-6745 NNP denotes mM
T2932 6739-6742 CD denotes 0.5
T2931 6737-6738 , denotes ,
T2930 6729-6737 NN denotes glycerol
T2929 6727-6728 NN denotes %
T2928 6726-6727 CD denotes 4
T2927 6724-6725 , denotes ,
T2926 6720-6724 NNP denotes NaCl
T2925 6717-6719 NNP denotes mM
T2924 6713-6716 CD denotes 0.5
T2923 6711-6712 , denotes ,
T2922 6706-6711 NNP denotes MgCl2
T2921 6703-6705 NNP denotes mM
T2920 6701-6702 CD denotes 1
T2919 6699-6700 , denotes ,
T2918 6698-6699 -RRB- denotes )
T2917 6695-6698 CD denotes 7.5
T2916 6692-6694 NNP denotes PH
T2915 6691-6692 -LRB- denotes (
T2914 6682-6690 NNP denotes Tris-HCl
T2913 6679-6681 NNP denotes mM
T2912 6676-6678 CD denotes 10
T2911 6665-6675 VBG denotes containing
T2910 6658-6664 NN denotes buffer
T2909 6650-6657 JJ denotes binding
T2908 6647-6649 NN denotes μL
T2907 6644-6646 CD denotes 20
T2906 6641-6643 IN denotes in
T2905 6628-6640 VBN denotes preincubated
T2904 6624-6627 VBD denotes was
T2903 6622-6623 -RRB- denotes )
T2902 6620-6622 NN denotes μg
T2901 6617-6619 CD denotes 20
T2900 6616-6617 -LRB- denotes (
T2899 6608-6615 NN denotes protein
T2898 6600-6607 NNP denotes Nuclear
T2897 6598-6599 . denotes .
T2896 6597-6598 -RRB- denotes )
T2895 6592-6597 NNP denotes China
T2894 6590-6591 , denotes ,
T2893 6583-6590 NNP denotes Beijing
T2892 6581-6582 , denotes ,
T2891 6573-6581 NNP denotes Biotech.
T2890 6568-6572 NNP denotes Free
T2889 6567-6568 -LRB- denotes (
T2888 6563-6566 NNP denotes ATP
T2887 6562-6563 : denotes -
T2886 6561-6562 NNP denotes ]
T2885 6556-6561 NNP denotes γ-32P
T2884 6555-6556 NNP denotes [
T2883 6549-6554 VBG denotes using
T2882 6542-6548 NN denotes kinase
T2881 6524-6541 NN denotes T4-polynucleotide
T2880 6521-6523 IN denotes by
T2879 6509-6520 VBN denotes end-labeled
T2878 6505-6508 VBD denotes was
T2877 6503-6504 -RRB- denotes )
T2876 6502-6503 NN denotes ′
T2875 6478-6502 NN denotes AGTTGAGGGGACTTTCCCAGGG-3
T2874 6477-6478 : denotes -
T2873 6476-6477 SYM denotes ′
T3968 9252-9257 NNP denotes Japan
T3967 9250-9251 , denotes ,
T3966 9245-9250 NNP denotes Tokyo
T3349 7857-7858 . denotes .
T3348 7849-7857 NN denotes standard
T3347 7840-7848 JJ denotes internal
T3346 7837-7839 DT denotes an
T3345 7834-7836 IN denotes as
T3344 7829-7833 VBN denotes used
T3343 7825-7828 VBD denotes was
T3342 7817-7824 NN denotes β-actin
T3341 7814-7816 IN denotes of
T3340 7808-7813 NN denotes level
T3339 7804-7807 DT denotes The
T3338 7802-7803 . denotes .
T3337 7795-7802 NN denotes program
T3336 7788-7794 NNP denotes ImageJ
T3335 7785-7787 IN denotes by
T3334 7776-7784 VBN denotes analyzed
T3333 7772-7775 VBD denotes was
T3332 7766-7771 NNS denotes bands
T3331 7762-7765 DT denotes the
T3330 7759-7761 IN denotes of
T3329 7749-7758 NN denotes intensity
T3328 7745-7748 DT denotes The
T3327 7743-7744 . denotes .
T3326 7728-7743 NN denotes electrophoresis
T3325 7724-7727 NN denotes gel
T3324 7716-7723 JJ denotes agarose
T3323 7713-7715 IN denotes by
T3322 7704-7712 VBN denotes detected
T3321 7699-7703 VBD denotes were
T3320 7690-7698 NNS denotes products
T3319 7686-7689 NNP denotes PCR
T3318 7684-7685 . denotes .
T3317 7674-7684 NNS denotes parameters
T3316 7670-7673 NNP denotes PCR
T3315 7666-7669 CC denotes and
T3314 7658-7665 NNS denotes primers
T3313 7654-7657 DT denotes the
T3312 7648-7653 VBZ denotes shows
T3311 7646-7647 CD denotes 1
T3310 7640-7645 NNP denotes Table
T3309 7637-7638 . denotes .
T3308 7629-7637 NN denotes protocol
T3307 7626-7628 POS denotes 's
T3306 7614-7626 NN denotes manufacturer
T3305 7610-7613 DT denotes the
T3304 7607-7609 TO denotes to
T3303 7597-7606 VBG denotes according
T3302 7595-7596 -RRB- denotes )
T3301 7592-7595 NNP denotes USA
T3300 7590-7591 , denotes ,
T3299 7588-7590 NNP denotes WI
T3298 7586-7587 , denotes ,
T3297 7579-7586 NNP denotes Promega
T3296 7578-7579 -LRB- denotes (
T3295 7574-7577 NNP denotes Mix
T3294 7567-7573 NNP denotes Master
T3293 7561-7566 NNP denotes Green
T3292 7555-7560 NNP denotes GoTaq
T3291 7550-7554 IN denotes with
T3290 7540-7549 VBN denotes conducted
T2872 6475-6476 CD denotes 5
T2871 6474-6475 -LRB- denotes (
T2870 6468-6473 NN denotes probe
T2869 6452-6467 NN denotes oligonucleotide
T2868 6442-6451 NNP denotes Consensus
T2867 6440-6441 . denotes .
T2866 6439-6440 -RRB- denotes )
T2865 6436-6439 NNP denotes USA
T2864 6434-6435 , denotes ,
T2863 6432-6434 NNP denotes WI
T2862 6430-6431 , denotes ,
T2861 6423-6430 NNP denotes Madison
T2860 6421-6422 , denotes ,
T2859 6414-6421 NNP denotes Promega
T2858 6413-6414 -LRB- denotes (
T2857 6406-6412 NN denotes system
T2856 6400-6405 NN denotes assay
T2855 6394-6399 NN denotes shift
T2854 6390-6393 NN denotes gel
T2853 6384-6389 VBG denotes using
T2852 6374-6383 VBN denotes performed
T2851 6370-6373 VBD denotes was
T2850 6365-6369 NNP denotes EMSA
T2849 6363-6364 . denotes .
T2848 6362-6363 -RRB- denotes )
T2847 6359-6362 NNP denotes USA
T2772 5965-5966 -RRB- denotes )
T2771 5962-5965 CD denotes 7.9
T2770 5959-5961 NNP denotes pH
T2769 5958-5959 -LRB- denotes (
T2768 5952-5957 NNP denotes HEPES
T2767 5949-5951 NNP denotes mM
T2766 5946-5948 CD denotes 20
T2765 5935-5945 VBG denotes containing
T2764 5928-5934 NN denotes buffer
T2763 5923-5927 IN denotes with
T2762 5913-5922 VBN denotes extracted
T2761 5908-5912 VBD denotes were
T2760 5899-5907 NNS denotes proteins
T2759 5891-5898 JJ denotes nuclear
T2758 5887-5890 DT denotes The
T2757 5885-5886 . denotes .
T2756 5871-5885 NN denotes centrifugation
T2755 5868-5870 IN denotes by
T2754 5859-5867 VBN denotes pelleted
T2753 5854-5858 VBD denotes were
T2752 5847-5853 NNS denotes nuclei
T2751 5843-5846 DT denotes the
T2750 5839-5842 CC denotes and
T2749 5837-5838 , denotes ,
T2748 5830-5837 NN denotes briefly
T2747 5821-5829 VBN denotes vortexed
T2746 5817-5820 CC denotes and
T2745 5811-5816 VBN denotes added
T2744 5807-5810 VBD denotes was
T2743 5802-5806 NNP denotes P-40
T2742 5794-5801 NNP denotes Nonidet
T2741 5792-5793 NN denotes %
T2740 5790-5792 CD denotes 10
T2739 5785-5789 RB denotes Then
T2738 5783-5784 . denotes .
T2737 5775-5783 NN denotes fluoride
T2736 5754-5774 NN denotes phenylmethylsulfonyl
T2735 5751-5753 NNP denotes mM
T2734 5747-5750 CD denotes 0.5
T2733 5743-5746 CC denotes and
T2732 5741-5742 , denotes ,
T2731 5738-5741 NNP denotes DTT
T2730 5735-5737 NNP denotes mM
T2729 5731-5734 CD denotes 1.0
T2728 5729-5730 , denotes ,
T2727 5725-5729 NNP denotes EGTA
T2726 5722-5724 NNP denotes mM
T2725 5718-5721 CD denotes 0.1
T2724 5716-5717 , denotes ,
T2723 5712-5716 NNP denotes EDTA
T2722 5709-5711 NNP denotes mM
T2721 5705-5708 CD denotes 0.1
T2720 5703-5704 , denotes ,
T2719 5700-5703 NNP denotes KCl
T2718 5697-5699 NNP denotes mM
T2717 5694-5696 CD denotes 10
T2716 5692-5693 , denotes ,
T2715 5691-5692 -RRB- denotes )
T2714 5688-5691 CD denotes 7.9
T2713 5685-5687 NNP denotes pH
T2712 5684-5685 -LRB- denotes (
T2711 5678-5683 NNP denotes HEPES
T2710 5675-5677 NNP denotes mM
T2709 5672-5674 CD denotes 10
T2708 5661-5671 VBG denotes containing
T2707 5654-5660 NN denotes buffer
T2706 5651-5653 IN denotes in
T2705 5639-5650 VBN denotes resuspended
T2704 5635-5638 VBD denotes was
T2703 5620-5634 NN denotes centrifugation
T2702 5617-5619 IN denotes by
T2701 5608-5616 VBN denotes obtained
T2700 5601-5607 NN denotes pellet
T2699 5596-5600 NN denotes cell
T2698 5592-5595 DT denotes The
T2697 5590-5591 . denotes .
T2696 5587-5590 NNP denotes PBS
T2695 5582-5586 JJ denotes cold
T2694 5577-5581 IN denotes into
T2693 5568-5576 VBG denotes scraping
T2692 5565-5567 IN denotes by
T2691 5555-5564 VBN denotes harvested
T2690 5551-5554 CC denotes and
T2689 5547-5550 NNP denotes PBS
T2688 5542-5546 IN denotes with
T2687 5535-5541 VBN denotes washed
T2686 5530-5534 VBD denotes were
T2685 5519-5529 NNS denotes astrocytes
T2684 5516-5518 IN denotes of
T2683 5505-5515 NNPS denotes Monolayers
T2682 5503-5504 -RRB- denotes )
T2681 5499-5503 NNP denotes EMSA
T2680 5498-5499 -LRB- denotes (
T2679 5492-5497 NNP denotes Assay
T2678 5486-5491 NNP denotes Shift
T2677 5477-5485 NNP denotes Mobility
T2676 5461-5476 NNP denotes Electrophoretic
T2675 5459-5460 . denotes .
T2674 5456-5459 CD denotes 2.4
T3965 9243-9244 , denotes ,
T3964 9234-9243 NNP denotes Hyperfilm
T3963 9229-9233 NNP denotes Fuji
T3962 9228-9229 -LRB- denotes (
T3961 9223-9227 NN denotes film
T3960 9210-9222 JJ denotes radiographic
T3959 9207-9209 TO denotes to
T3958 9199-9206 VBN denotes exposed
T3957 9195-9198 CC denotes and
T3956 9193-9194 -RRB- denotes )
T3955 9191-9193 NNP denotes UK
T3954 9189-9190 , denotes ,
T3953 9184-9189 NNP denotes Bucks
T3952 9182-9183 , denotes ,
T3951 9171-9182 NNP denotes Biosciences
T3950 9162-9170 NNP denotes Amersham
T3949 9161-9162 -LRB- denotes (
T3948 9154-9160 NN denotes system
T3947 9144-9153 NN denotes detection
T3946 9142-9143 -RRB- denotes )
T3945 9139-9142 NNP denotes ECL
T3944 9138-9139 -LRB- denotes (
T3943 9120-9137 NN denotes chemiluminescence
T3942 9111-9119 JJ denotes enhanced
T3941 9106-9110 IN denotes with
T3940 9096-9105 VBN denotes incubated
T3939 9091-9095 VBD denotes were
T3938 9085-9090 NNS denotes Blots
T3937 9082-9084 NN denotes h.
T3936 9080-9081 CD denotes 1
T3935 9076-9079 IN denotes for
T3934 9067-9075 NN denotes dilution
T3933 9062-9066 CD denotes 2000
T3932 9060-9061 : denotes :
T3931 9058-9059 CD denotes 1
T3930 9056-9057 DT denotes a
T3929 9053-9055 IN denotes at
T3928 9044-9052 NN denotes antibody
T3927 9034-9043 JJ denotes secondary
T3926 9016-9033 JJ denotes peroxidase-linked
T3925 9004-9015 JJ denotes horseradish
T3924 9000-9003 DT denotes the
T3923 8995-8999 IN denotes with
T3922 8987-8994 VBN denotes covered
T3921 8983-8986 CC denotes and
T3920 8976-8982 NN denotes buffer
T3919 8968-8975 VBG denotes washing
T3918 8964-8967 DT denotes the
T3917 8961-8963 IN denotes in
T3916 8955-8960 NNS denotes times
T3915 8949-8954 CD denotes three
T3914 8942-8948 VBN denotes washed
T3913 8937-8941 VBD denotes were
T3912 8931-8936 NNS denotes blots
T3911 8927-8930 DT denotes The
T3910 8925-8926 . denotes .
T3909 8914-8925 NN denotes temperature
T3908 8909-8913 NN denotes room
T3907 8906-8908 IN denotes at
T3906 8904-8905 NN denotes h
T3905 8902-8903 CD denotes 1
T3904 8898-8901 IN denotes for
T3903 8892-8897 NNS denotes blots
T3902 8888-8891 DT denotes the
T3901 8885-8887 TO denotes to
T3900 8879-8884 VBD denotes added
T3899 8874-8878 RB denotes then
T3898 8870-8873 CC denotes and
T3897 8863-8869 NN denotes buffer
T3896 8854-8862 VBG denotes blocking
T3895 8851-8853 IN denotes in
T2846 6357-6358 , denotes ,
T2845 6355-6357 NNP denotes IL
T2844 6353-6354 , denotes ,
T2843 6345-6353 NNP denotes Rockford
T2842 6343-6344 , denotes ,
T2841 6331-6343 NNP denotes Biochemicals
T2840 6324-6330 NNP denotes Pierce
T2839 6323-6324 -LRB- denotes (
T2838 6314-6322 NN denotes standard
T2837 6310-6313 DT denotes the
T2836 6307-6309 IN denotes as
T2835 6299-6306 NN denotes albumin
T2834 6293-6298 NN denotes serum
T2833 6286-6292 JJ denotes bovine
T2832 6281-6285 IN denotes with
T2831 6277-6280 NN denotes kit
T2830 6271-6276 NN denotes assay
T2829 6266-6270 NN denotes acid
T2828 6252-6265 JJ denotes bicinchoninic
T2827 6250-6251 DT denotes a
T2826 6244-6249 VBG denotes using
T2825 6233-6243 VBN denotes determined
T2824 6229-6232 VBD denotes was
T2823 6215-6228 NN denotes concentration
T2822 6207-6214 NN denotes Protein
T2821 6205-6206 . denotes .
T2820 6202-6205 NN denotes use
T2819 6196-6201 IN denotes until
T2818 6194-6195 NNP denotes C
T2817 6193-6194 CD denotes °
T2816 6191-6193 CD denotes 80
T2815 6187-6189 IN denotes at
T2814 6180-6186 VBN denotes stored
T2813 6176-6179 VBD denotes was
T2812 6167-6175 NNS denotes proteins
T2811 6159-6166 JJ denotes nuclear
T2810 6155-6158 DT denotes the
T2809 6144-6154 VBG denotes containing
T2808 6132-6143 JJ denotes supernatant
T2807 6128-6131 DT denotes the
T2806 6124-6127 CC denotes and
T2805 6122-6123 , denotes ,
T2804 6119-6122 NN denotes rpm
T2803 6113-6118 CD denotes 14000
T2802 6110-6112 IN denotes at
T2801 6095-6109 NN denotes centrifugation
T2800 6092-6094 IN denotes by
T2799 6084-6091 VBN denotes removed
T2798 6080-6083 VBD denotes was
T2797 6071-6079 NN denotes material
T2796 6061-6070 NNP denotes Insoluble
T2795 6059-6060 . denotes .
T2794 6051-6059 NN denotes fluoride
T2793 6030-6050 NN denotes phenylmethylsulfonyl
T2792 6027-6029 NNP denotes mM
T2791 6023-6026 CD denotes 1.0
T2790 6019-6022 CC denotes and
T2789 6017-6018 , denotes ,
T2788 6014-6017 NNP denotes DTT
T2787 6011-6013 NNP denotes mM
T2786 6007-6010 CD denotes 1.0
T2785 6005-6006 , denotes ,
T2784 6001-6005 NNP denotes EGTA
T2783 5998-6000 NNP denotes mM
T2782 5994-5997 CD denotes 1.0
T2781 5992-5993 , denotes ,
T2780 5988-5992 NNP denotes EDTA
T2779 5985-5987 NNP denotes mM
T2778 5981-5984 CD denotes 1.0
T2777 5979-5980 , denotes ,
T2776 5975-5979 NNP denotes NaCl
T2775 5972-5974 NNP denotes mM
T2774 5968-5971 CD denotes 0.4
T2773 5966-5967 , denotes ,
T2294 5453-5454 . denotes .
T2293 5451-5453 NNP denotes SD
T2292 5449-5450 NN denotes ±
T2291 5446-5448 NN denotes nm
T2290 5442-5445 CD denotes 450
T2289 5439-5441 IN denotes at
T2288 5433-5438 NN denotes value
T2287 5430-5432 NNP denotes OD
T2286 5427-5429 IN denotes of
T2285 5422-5426 NN denotes mean
T2284 5419-5421 IN denotes as
T2283 5409-5418 VBN denotes expressed
T2282 5404-5408 VBD denotes were
T2281 5396-5403 NNS denotes Results
T2280 5394-5395 . denotes .
T2279 5382-5394 NN denotes sextuplicate
T2278 5379-5381 IN denotes in
T2260 5280-5281 DT denotes a
T2259 5274-5279 VBG denotes using
T2258 5271-5273 NN denotes nm
T2257 5267-5270 CD denotes 450
T2256 5264-5266 IN denotes at
T2253 5249-5254 NN denotes value
T2252 5246-5248 NNP denotes OD
T2251 5241-5245 RB denotes Then
T2250 5239-5240 . denotes .
T2249 5238-5239 NN denotes h
T2248 5236-5237 CD denotes 1
T2247 5232-5235 IN denotes for
T2246 5222-5231 VBN denotes incubated
T2245 5218-5221 CC denotes and
T2244 5213-5217 NN denotes well
T2243 5207-5212 DT denotes every
T2242 5202-5206 IN denotes into
T2241 5196-5201 VBN denotes added
T2240 5192-5195 VBD denotes was
T2239 5186-5191 NNP denotes CCK-8
T2238 5183-5185 NNP denotes μL
T2237 5180-5182 CD denotes 10
T2236 5178-5179 , denotes ,
T2235 5171-5178 RB denotes Briefly
T2234 5169-5170 . denotes .
T2233 5161-5169 NN denotes protocol
T2232 5158-5160 POS denotes 's
T2231 5146-5158 NN denotes manufacturer
T2230 5142-5145 DT denotes the
T2229 5139-5141 TO denotes to
T2228 5129-5138 VBG denotes according
T2227 5121-5128 NN denotes scratch
T2226 5115-5120 IN denotes after
T2225 5113-5114 NN denotes h
T2224 5110-5112 CD denotes 24
T2223 5104-5109 NN denotes assay
T2222 5102-5103 -RRB- denotes )
T2221 5097-5102 NNP denotes Japan
T2220 5095-5096 , denotes ,
T2219 5088-5095 NNP denotes Dojindo
T2218 5087-5088 -LRB- denotes (
T2217 5085-5086 -RRB- denotes )
T2216 5080-5085 NN denotes CCK-8
T2215 5079-5080 -LRB- denotes (
T2214 5073-5078 JJ denotes kit-8
T2213 5064-5072 VBG denotes counting
T2212 5059-5063 NN denotes cell
T2211 5056-5058 IN denotes by
T2210 5047-5055 VBN denotes assessed
T2209 5043-5046 VBD denotes was
T2208 5037-5042 NN denotes death
T2207 5032-5036 NN denotes cell
T2206 5022-5031 NNP denotes Astrocyte
T2205 5020-5021 . denotes .
T2204 5015-5020 IN denotes above
T2203 5005-5014 VBN denotes mentioned
T2202 5002-5004 IN denotes as
T2201 4994-5001 VBN denotes scrated
T2200 4990-4993 VBD denotes was
T2199 4985-4989 NN denotes cell
T2198 4981-4984 DT denotes the
T2197 4976-4980 RB denotes Then
T2196 4974-4975 . denotes .
T2195 4968-4974 VB denotes adhere
T2194 4965-4967 TO denotes to
T2193 4963-4964 NN denotes h
T2192 4960-4962 CD denotes 24
T2191 4956-4959 IN denotes for
T2190 4945-4955 VBN denotes cultivated
T2189 4941-4944 CC denotes and
T2188 4934-4940 NNS denotes plates
T2187 4926-4933 NN denotes culture
T2186 4918-4925 JJ denotes 96-well
T2185 4915-4917 TO denotes to
T2184 4908-4914 VBN denotes seeded
T2183 4903-4907 VBD denotes were
T2182 4897-4902 NNS denotes cells
T2181 4888-4896 JJ denotes 103/well
T2180 4886-4887 CD denotes ×
T2179 4884-4885 CD denotes 1
T2178 4878-4883 NNP denotes Assay
T2177 4872-4877 NNP denotes CCK-8
T2176 4869-4871 IN denotes by
T2175 4857-4868 NNP denotes Measurement
T2174 4851-4856 NNP denotes Death
T2173 4846-4850 NNP denotes Cell
T2172 4844-4845 . denotes .
T2171 4841-4844 CD denotes 2.3
T1737 4033-4036 CC denotes and
T1736 4023-4032 NN denotes microglia
T1735 4019-4022 DT denotes the
T1734 4011-4018 VB denotes deplete
T1733 4008-4010 TO denotes to
T1732 4006-4007 NN denotes h
T1731 4004-4005 CD denotes 4
T1730 4000-4003 IN denotes for
T1729 3996-3999 NN denotes rpm
T1728 3992-3995 CD denotes 150
T1727 3989-3991 IN denotes at
T1726 3982-3988 VBN denotes shaken
T1725 3977-3981 VBD denotes were
T1724 3970-3976 NNS denotes dishes
T1723 3967-3969 CC denotes or
T1722 3960-3966 NNS denotes flasks
T1721 3956-3959 DT denotes the
T1720 3951-3955 RB denotes Then
T1719 3949-3950 . denotes .
T1718 3939-3949 NN denotes confluence
T1717 3933-3938 VB denotes reach
T1716 3930-3932 TO denotes to
T1715 3925-3929 NNS denotes days
T1714 3923-3924 CD denotes 7
T1713 3917-3922 IN denotes about
T1712 3913-3916 IN denotes for
T1711 3904-3912 VBD denotes expanded
T1710 3893-3903 NNP denotes Astrocytes
T1709 3891-3892 . denotes .
T1708 3887-3891 NN denotes week
T1707 3885-3886 DT denotes a
T1706 3879-3884 RB denotes twice
T1705 3866-3878 JJ denotes half-changed
T1704 3862-3865 VBD denotes was
T1703 3855-3861 NN denotes medium
T1702 3847-3854 NN denotes culture
T1701 3838-3846 JJ denotes complete
T1700 3834-3837 DT denotes the
T1699 3830-3833 CC denotes And
T1698 3828-3829 . denotes .
T1697 3825-3828 CD denotes CO2
T1696 3823-3824 NN denotes %
T1695 3822-3823 CD denotes 5
T1694 3817-3821 IN denotes with
T1693 3815-3816 NNP denotes C
T1692 3814-3815 CD denotes °
T1691 3812-3814 CD denotes 37
T1690 3809-3811 IN denotes at
T1689 3798-3808 VBN denotes cultivated
T1688 3793-3797 VBD denotes were
T1687 3787-3792 NNS denotes cells
T1686 3783-3786 DT denotes the
T1685 3778-3782 RB denotes Then
T1684 3776-3777 . denotes .
T1683 3771-3776 NNS denotes cells
T1682 3760-3770 JJ denotes epithelial
T1681 3751-3759 JJ denotes residual
T1680 3747-3750 DT denotes the
T1679 3737-3746 VBG denotes depleting
T1678 3733-3736 IN denotes for
T1677 3731-3732 -RRB- denotes )
T1676 3728-3731 NNP denotes USA
T1675 3726-3727 , denotes ,
T1674 3720-3726 NNP denotes Costar
T1673 3719-3720 -LRB- denotes (
T1672 3714-3718 NN denotes dish
T1671 3711-3713 CC denotes or
T1670 3705-3710 NN denotes flask
T1669 3701-3704 JJ denotes new
T1668 3699-3700 DT denotes a
T1667 3696-3698 TO denotes to
T1666 3684-3695 VBN denotes transferred
T1665 3680-3683 VBD denotes was
T1664 3668-3679 NN denotes supernatant
T1663 3664-3667 DT denotes the
T1662 3662-3663 , denotes ,
T1661 3661-3662 NN denotes h
T1660 3659-3660 CD denotes 1
T1659 3655-3658 IN denotes for
T1658 3644-3654 NN denotes incubation
T1657 3638-3643 IN denotes After
T1656 3636-3637 . denotes .
T1655 3635-3636 -RRB- denotes )
T1654 3629-3635 NN denotes medium
T1653 3621-3628 NN denotes culture
T1652 3612-3620 JJ denotes complete
T1651 3611-3612 -LRB- denotes (
T1650 3598-3610 NNP denotes streptomycin
T1649 3595-3597 NNP denotes mL
T1648 3594-3595 NN denotes /
T1647 3592-3594 NN denotes μg
T1646 3590-3591 CD denotes 5
T1645 3588-3589 , denotes ,
T1644 3578-3588 NN denotes penicillin
T1643 3569-3577 JJ denotes units/mL
T1642 3567-3568 CD denotes 5
T1641 3563-3566 CC denotes and
T1640 3559-3562 NNP denotes FBS
T1639 3557-3558 NN denotes %
T1638 3555-3557 CD denotes 20
T1637 3550-3554 IN denotes with
T1636 3545-3549 NNP denotes DMEM
T1635 3542-3544 IN denotes in
T1634 3529-3541 NN denotes dissociation
T1633 3518-3528 JJ denotes mechanical
T1632 3516-3517 DT denotes a
T1631 3513-3515 IN denotes by
T1630 3504-3512 VBN denotes followed
T1629 3502-3503 , denotes ,
T1628 3499-3502 NNP denotes FBS
T1627 3497-3498 NN denotes %
T1626 3495-3497 CD denotes 10
T1625 3490-3494 IN denotes with
T1624 3485-3489 NNP denotes DMEM
T1623 3482-3484 IN denotes in
T1622 3476-3481 RB denotes twice
T1621 3469-3475 VBN denotes rinsed
T1620 3465-3468 VBD denotes was
T1619 3458-3464 NN denotes tissue
T1618 3454-3457 DT denotes the
T1617 3452-3453 , denotes ,
T1616 3443-3452 NN denotes digestion
T1615 3437-3442 IN denotes After
T1614 3435-3436 . denotes .
T1613 3428-3435 NNS denotes minutes
T1612 3424-3427 JJ denotes few
T1611 3418-3423 DT denotes every
T1610 3409-3417 VBD denotes agitated
T1609 3405-3408 CC denotes and
T1608 3403-3404 , denotes ,
T1607 3402-3403 NNP denotes C
T1606 3401-3402 CD denotes °
T1605 3399-3401 CD denotes 37
T1604 3396-3398 IN denotes at
T1603 3388-3395 NNS denotes minutes
T1602 3385-3387 CD denotes 10
T1601 3381-3384 IN denotes for
T1600 3373-3380 NN denotes trypsin
T1599 3371-3372 NN denotes %
T1598 3368-3371 CD denotes 0.5
T1597 3365-3367 IN denotes in
T1596 3355-3364 VBN denotes incubated
T1595 3353-3354 , denotes ,
T1594 3348-3353 NN denotes blade
T1593 3340-3347 NN denotes scalpel
T1592 3338-3339 DT denotes a
T1591 3333-3337 IN denotes with
T1574 3237-3240 RP denotes out
T1573 3227-3236 VBN denotes dissected
T1572 3222-3226 VBD denotes were
T1571 3213-3221 NNS denotes cortices
T1570 3209-3212 DT denotes the
T1569 3207-3208 , denotes ,
T1568 3195-3207 NN denotes decapitation
T1567 3185-3194 VBG denotes Following
T1566 3183-3184 . denotes .
T1565 3182-3183 -RRB- denotes )
T1564 3174-3182 NN denotes genotype
T1563 3169-3173 DT denotes each
T1562 3165-3168 IN denotes for
T1561 3160-3164 NNS denotes mice
T1560 3158-3159 CD denotes 6
T1559 3157-3158 -LRB- denotes (
T1558 3152-3156 NNS denotes mice
T1557 3150-3151 -RRB- denotes )
T1556 3148-3150 NNP denotes KO
T1555 3147-3148 -LRB- denotes (
T1554 3138-3146 NN denotes knockout
T1553 3134-3137 CC denotes and
T1552 3132-3133 -RRB- denotes )
T1551 3130-3132 NNP denotes WT
T1550 3129-3130 -LRB- denotes (
T1549 3124-3128 NN denotes type
T1548 3119-3123 JJ denotes wide
T2277 5369-5378 VBN denotes performed
T2276 5364-5368 VBD denotes were
T2275 5351-5363 NNS denotes measurements
T2274 5347-5350 DT denotes All
T2273 5345-5346 . denotes .
T2272 5344-5345 -RRB- denotes )
T2271 5341-5344 NNP denotes USA
T2270 5339-5340 , denotes ,
T2269 5337-5339 NNP denotes CA
T2268 5335-5336 , denotes ,
T2267 5323-5335 NNPS denotes Laboratories
T2266 5315-5322 NNP denotes Bio-Rad
T2265 5314-5315 -LRB- denotes (
T2264 5307-5313 NN denotes reader
T2263 5296-5306 NN denotes microplate
T2262 5290-5295 NNP denotes ELISA
T2261 5282-5289 NNP denotes Bio-Rad
T1547 3114-3118 JJ denotes Nrf2
T1546 3104-3113 JJ denotes 2-day-old
T1545 3094-3103 JJ denotes postnatal
T1544 3089-3093 IN denotes from
T1543 3080-3088 VBN denotes obtained
T1542 3075-3079 VBD denotes were
T1541 3064-3074 NNS denotes astrocytes
T1540 3056-3063 JJ denotes Primary
T1539 3054-3055 . denotes .
T1538 3053-3054 -RRB- denotes )
T1537 3050-3053 NNP denotes USA
T1536 3048-3049 , denotes ,
T1535 3046-3048 NNP denotes MD
T1534 3044-3045 , denotes ,
T1533 3035-3044 NNP denotes Baltimore
T1532 3033-3034 , denotes ,
T1531 3023-3033 NNP denotes University
T1530 3015-3022 NNP denotes Hopkins
T1529 3009-3014 NNP denotes Johns
T1528 3008-3009 -LRB- denotes (
T1527 3000-3007 NNP denotes Kensler
T1526 2997-2999 NNP denotes W.
T1525 2990-2996 NNP denotes Thomas
T1524 2986-2989 NNP denotes Dr.
T1523 2983-2985 IN denotes by
T1927 4332-4335 NNP denotes TBI
T1926 4329-4331 IN denotes of
T1925 4323-4328 NNP denotes Model
T1924 4317-4322 NNP denotes Vitro
T1923 4314-4316 IN denotes In
T1922 4312-4313 . denotes .
T1921 4309-4312 CD denotes 2.2
T1785 4306-4307 . denotes .
T1784 4299-4306 NNS denotes studies
T1783 4289-4298 JJ denotes following
T1782 4285-4288 DT denotes the
T1781 4282-4284 IN denotes in
T1780 4277-4281 VBN denotes used
T1779 4272-4276 VBD denotes were
T1778 4260-4271 NNS denotes generations
T1777 4256-4259 CD denotes 2-3
T1776 4252-4255 IN denotes for
T1775 4243-4251 VBD denotes passaged
T1774 4237-4242 NNS denotes Cells
T1773 4235-4236 . denotes .
T1772 4234-4235 -RRB- denotes )
T1771 4231-4234 NNP denotes USA
T1770 4229-4230 , denotes ,
T1769 4227-4229 NNP denotes CA
T1768 4225-4226 , denotes ,
T1767 4212-4225 NNP denotes Biotechnology
T1766 4207-4211 NNP denotes Cruz
T1765 4201-4206 NNP denotes Santa
T1764 4199-4200 , denotes ,
T1763 4190-4199 JJ denotes sc-166481
T1762 4189-4190 -LRB- denotes (
T1761 4184-4188 NNP denotes GFAP
T1760 4180-4183 IN denotes for
T1759 4163-4179 NN denotes immunoreactivity
T1758 4160-4162 IN denotes by
T1757 4149-4159 VBN denotes determined
T1756 4144-4148 VBD denotes were
T1755 4138-4143 WDT denotes which
T1754 4136-4137 , denotes ,
T1753 4126-4136 NNS denotes astrocytes
T1752 4119-4125 RB denotes mainly
T1751 4114-4118 VBD denotes were
T1750 4105-4113 NNS denotes cultures
T1749 4095-4104 VBG denotes resulting
T1748 4091-4094 DT denotes the
T1747 4089-4090 , denotes ,
T1746 4082-4089 VBG denotes shaking
T1745 4076-4081 IN denotes After
T1744 4074-4075 . denotes .
T1743 4066-4074 NNS denotes cultures
T1742 4062-4065 DT denotes the
T1741 4057-4061 IN denotes from
T1740 4051-4056 NNS denotes cells
T1739 4042-4050 JJ denotes adherent
T1738 4037-4041 JJR denotes less
T1186 2850-2851 . denotes .
T1185 2844-2850 NN denotes injury
T1184 2836-2843 NN denotes scratch
T1183 2830-2835 IN denotes after
T1182 2825-2829 NNS denotes mice
T1181 2814-2824 JJ denotes transgenic
T1180 2809-2813 IN denotes from
T1179 2798-2808 NNS denotes astrocytes
T1178 2789-2797 JJ denotes cultured
T1177 2781-2788 JJ denotes primary
T1176 2778-2780 IN denotes in
T1175 2768-2777 NNS denotes cytokines
T1174 2752-2767 JJ denotes proinflammatory
T1173 2749-2751 IN denotes of
T1172 2738-2748 NN denotes expression
T1171 2734-2737 DT denotes the
T1170 2731-2733 IN denotes on
T1169 2726-2730 NNP denotes Nrf2
T1168 2723-2725 IN denotes of
T1167 2713-2722 NN denotes influence
T1166 2709-2712 DT denotes the
T1165 2700-2708 VBD denotes analysed
T1164 2697-2699 PRP denotes we
T1163 2692-2696 RB denotes here
T1162 2690-2691 , denotes ,
T1161 2681-2690 RB denotes Therefore
T1160 2679-2680 . denotes .
T1159 2676-2679 NNP denotes TBI
T1158 2670-2675 IN denotes after
T1157 2659-2669 NNS denotes astrocytes
T1156 2656-2658 IN denotes in
T1155 2643-2655 NN denotes inflammation
T1154 2639-2642 CC denotes and
T1153 2634-2638 NNP denotes Nrf2
T1152 2626-2633 IN denotes between
T1151 2613-2625 NN denotes relationship
T1150 2609-2612 DT denotes the
T1149 2603-2608 IN denotes about
T1148 2597-2602 VBN denotes known
T1147 2594-2596 VBZ denotes is
T1146 2587-2593 NNP denotes Little
T1145 2585-2586 . denotes .
T1144 2581-2585 NN denotes vivo
T1143 2578-2580 IN denotes in
T1142 2568-2577 VBN denotes performed
T1141 2564-2567 VBP denotes are
T1140 2556-2563 NNS denotes studies
T1139 2541-2555 JJ denotes aforementioned
T1138 2537-2540 DT denotes the
T1137 2533-2536 PDT denotes all
T1136 2531-2532 , denotes ,
T1135 2527-2531 NN denotes fact
T1134 2524-2526 IN denotes In
T1133 2522-2523 . denotes .
T1132 2515-2522 NN denotes disease
T1131 2512-2514 CC denotes or
T1130 2505-2511 NN denotes injury
T1129 2502-2504 TO denotes to
T1128 2491-2501 VBG denotes responding
T1127 2487-2490 CC denotes and
T1126 2476-2486 NN denotes physiology
T1125 2470-2475 NN denotes brain
T1124 2463-2469 JJ denotes normal
T1123 2451-2462 VBG denotes maintaining
T1122 2448-2450 IN denotes in
T1121 2443-2447 NN denotes role
T1120 2434-2442 JJ denotes critical
T1119 2432-2433 DT denotes a
T1118 2427-2431 VBP denotes play
T1117 2416-2426 NNS denotes astrocytes
T1116 2411-2415 IN denotes that
T1115 2400-2410 VBN denotes documented
T1114 2395-2399 RB denotes well
T1113 2392-2394 VBZ denotes is
T1112 2389-2391 PRP denotes It
T1111 2387-2388 . denotes .
T1110 2386-2387 NNP denotes ]
T1109 2385-2386 CD denotes 6
T1108 2384-2385 NNP denotes [
T1107 2382-2383 -RRB- denotes )
T1106 2379-2382 NNP denotes SFN
T1105 2378-2379 -LRB- denotes (
T1104 2365-2377 NN denotes sulforaphane
T1103 2362-2364 IN denotes as
T1102 2357-2361 JJ denotes such
T1101 2355-2356 , denotes ,
T1100 2347-2355 NNS denotes inducers
T1099 2343-2346 PRP$ denotes its
T1098 2340-2342 IN denotes by
T1097 2335-2339 JJ denotes Nrf2
T1096 2332-2334 IN denotes of
T1095 2326-2331 NN denotes level
T1094 2322-2325 DT denotes the
T1093 2312-2321 VBG denotes elevating
T1092 2304-2311 IN denotes through
T1091 2293-2303 VBN denotes extenuated
T1090 2290-2292 VB denotes be
T1089 2286-2289 MD denotes can
T1088 2273-2285 NN denotes inflammation
T1087 2268-2272 JJ denotes such
T1086 2266-2267 , denotes ,
T1085 2258-2266 NN denotes contrary
T1084 2254-2257 DT denotes the
T1083 2251-2253 IN denotes On
T1082 2249-2250 . denotes .
T1081 2248-2249 NNP denotes ]
T1080 2247-2248 CD denotes 5
T1079 2246-2247 VBP denotes [
T1078 2241-2245 NNS denotes mice
T1077 2232-2240 NN denotes wildtype
T1076 2229-2231 IN denotes in
T1075 2224-2228 IN denotes than
T1074 2219-2223 NNS denotes mice
T1073 2210-2218 NN denotes knockout
T1072 2205-2209 JJ denotes Nrf2
T1071 2202-2204 IN denotes in
T1070 2195-2201 NN denotes damage
T1069 2184-2194 VBN denotes aggravated
T1068 2179-2183 RBR denotes more
T1067 2172-2178 VB denotes induce
T1066 2166-2171 MD denotes could
T1065 2162-2165 NNP denotes TBI
T1064 2157-2161 IN denotes that
T1063 2150-2156 VBN denotes showed
T1062 2145-2149 RB denotes also
T1061 2141-2144 VBD denotes was
T1060 2138-2140 PRP denotes It
T1059 2136-2137 . denotes .
T2022 4838-4839 . denotes .
T2021 4833-4838 NN denotes group
T2020 4828-4832 NN denotes sham
T2019 4825-4827 IN denotes as
T2018 4819-4824 VBN denotes named
T2017 4815-4818 CC denotes and
T2016 4807-4814 NN denotes control
T2015 4801-4806 JJ denotes blank
T2014 4798-4800 IN denotes as
T2013 4793-4797 VBN denotes used
T2012 4788-4792 VBD denotes were
T2011 4786-4787 , denotes ,
T2010 4779-4786 NN denotes scratch
T2009 4770-4778 VBN denotes received
T2008 4766-4769 RB denotes not
T2007 4762-4765 VBD denotes did
T2006 4756-4761 WDT denotes which
T2005 4754-4755 , denotes ,
T2004 4749-4754 NNS denotes Cells
T2003 4747-4748 . denotes .
T2002 4742-4747 NN denotes group
T2001 4738-4741 NNP denotes TBI
T2000 4735-4737 IN denotes as
T1999 4729-4734 VBN denotes named
T1998 4725-4728 CC denotes and
T1997 4723-4724 , denotes ,
T1996 4717-4723 NN denotes medium
T1995 4709-4716 NN denotes culture
T1994 4700-4708 JJ denotes complete
T1993 4695-4699 IN denotes with
T1992 4686-4694 VBN denotes cultured
T1991 4684-4685 , denotes ,
T1990 4681-4684 NNP denotes PBS
T1989 4673-4680 JJ denotes sterile
T1988 4668-4672 IN denotes with
T1987 4662-4667 RB denotes twice
T1986 4655-4661 VBN denotes washed
T1985 4650-4654 VBD denotes were
T1984 4644-4649 NNS denotes cells
T1983 4642-4643 , denotes ,
T1982 4635-4642 NN denotes scratch
T1981 4629-4634 IN denotes after
T1980 4617-4628 RB denotes Immediately
T1979 4615-4616 . denotes .
T1978 4612-4615 NN denotes gap
T1977 4607-4611 JJ denotes wide
T1976 4604-4606 NN denotes mm
T1975 4600-4603 CD denotes 0.5
T1974 4598-4599 DT denotes a
T1973 4595-4597 IN denotes of
T1972 4585-4594 NN denotes formation
T1971 4581-4584 DT denotes the
T1970 4578-4580 IN denotes in
T1969 4568-4577 VBG denotes resulting
T1968 4566-4567 , denotes ,
T1967 4560-4566 NN denotes needle
T1966 4552-4559 NN denotes syringe
T1965 4548-4551 JJ denotes 26G
T1964 4540-4547 JJ denotes sterile
T1963 4538-4539 DT denotes a
T1962 4533-4537 IN denotes with
T1961 4523-4532 VBN denotes scratched
T1960 4519-4522 VBD denotes was
T1959 4509-4518 NN denotes monolayer
T1958 4504-4508 NN denotes cell
T1957 4500-4503 DT denotes The
T1956 4498-4499 . denotes .
T1955 4488-4498 NN denotes confluence
T1954 4485-4487 TO denotes to
T1953 4479-4484 VBN denotes grown
T1952 4475-4478 CC denotes and
T1951 4468-4474 NNS denotes plates
T1950 4461-4467 JJ denotes 6-well
T1949 4458-4460 IN denotes in
T1948 4450-4457 VBN denotes planted
T1947 4445-4449 VBD denotes were
T1946 4434-4444 NNS denotes Astrocytes
T1945 4432-4433 . denotes .
T1944 4431-4432 NNP denotes ]
T1943 4430-4431 CD denotes 7
T1942 4429-4430 NN denotes [
T1941 4422-4428 NN denotes report
T1940 4413-4421 JJ denotes previous
T1939 4411-4412 DT denotes a
T1938 4408-4410 IN denotes in
T1937 4405-4407 IN denotes as
T1936 4395-4404 VBN denotes performed
T1935 4391-4394 VBD denotes was
T1934 4384-4390 NN denotes injury
T1933 4376-4383 NN denotes scratch
T1932 4366-4375 NNP denotes Astrocyte
T1931 4359-4365 NNP denotes Injury
T1930 4351-4358 NNP denotes Scratch
T1929 4348-4350 IN denotes by
T1928 4336-4347 VBN denotes Established
T1058 2135-2136 -RRB- denotes )
T1057 2132-2135 NNP denotes TBI
T1056 2131-2132 -LRB- denotes (
T1055 2124-2130 NN denotes injury
T1054 2118-2123 NN denotes brain
T1053 2108-2117 JJ denotes traumatic
T1052 2102-2107 IN denotes after
T1051 2096-2101 NN denotes brain
T1050 2092-2095 DT denotes the
T1049 2089-2091 IN denotes in
T1048 2076-2088 NN denotes inflammation
T1047 2072-2075 DT denotes the
T1046 2062-2071 VBZ denotes regulates
T1045 2057-2061 JJ denotes Nrf2
T1044 2052-2056 IN denotes that
T1043 2039-2051 VBN denotes demonstrated
T1042 2034-2038 VBP denotes have
T1041 2026-2033 NNS denotes studies
T1040 2018-2025 JJ denotes Several
T1039 2016-2017 . denotes .
T1038 2011-2016 NNS denotes genes
T1037 1999-2010 JJ denotes antioxidant
T1036 1996-1998 IN denotes of
T1035 1990-1995 NNS denotes kinds
T1034 1985-1989 JJ denotes many
T1033 1975-1984 VBZ denotes regulates
T526 1288-1289 . denotes .
T525 1278-1288 NNS denotes astrocytes
T524 1275-1277 IN denotes in
T523 1265-1274 NNS denotes cytokines
T522 1249-1264 JJ denotes proinflammatory
T521 1238-1248 JJ denotes downstream
T520 1234-1237 CC denotes and
T519 1228-1233 NNP denotes NF-κB
T518 1225-1227 IN denotes of
T517 1214-1224 NN denotes activation
T516 1206-1213 IN denotes through
T515 1193-1205 NN denotes inflammation
T514 1182-1192 JJ denotes aggressive
T513 1177-1181 RBR denotes more
T512 1170-1176 VB denotes induce
T511 1166-1169 MD denotes may
T510 1161-1165 NNP denotes Nrf2
T509 1158-1160 IN denotes of
T508 1150-1157 NN denotes absence
T507 1146-1149 DT denotes the
T506 1141-1145 IN denotes that
T505 1133-1140 VBP denotes suggest
T504 1125-1132 NNS denotes results
T503 1119-1124 DT denotes These
T502 1117-1118 . denotes .
T501 1107-1117 NNS denotes astrocytes
T500 1104-1106 NNP denotes WT
T499 1101-1103 IN denotes in
T498 1096-1100 DT denotes that
T497 1091-1095 IN denotes than
T496 1080-1090 NNS denotes astrocytes
T495 1077-1079 NNP denotes KO
T494 1072-1076 NNP denotes Nrf2
T493 1069-1071 IN denotes in
T492 1059-1068 JJ denotes prominent
T491 1054-1058 JJR denotes more
T490 1049-1053 RB denotes much
T489 1045-1048 VBD denotes was
T488 1036-1044 NN denotes tendency
T487 1031-1035 JJ denotes such
T486 1027-1030 CC denotes And
T485 1025-1026 . denotes .
T484 1017-1025 VBN denotes elevated
T483 1012-1016 RB denotes also
T482 1007-1011 VBD denotes were
T481 1002-1006 CD denotes MMP9
T480 999-1001 IN denotes of
T479 990-998 NN denotes activity
T478 986-989 CC denotes and
T477 975-985 NN denotes expression
T476 971-974 DT denotes The
T475 969-970 . denotes .
T474 962-969 NN denotes scratch
T473 956-961 IN denotes after
T472 954-955 NN denotes h
T471 951-953 CD denotes 24
T470 948-950 IN denotes at
T469 936-947 VBN denotes upregulated
T468 931-935 VBD denotes were
T467 925-930 IN denotes above
T466 915-924 VBN denotes mentioned
T465 905-914 NNS denotes cytokines
T464 889-904 JJ denotes proinflammatory
T463 886-888 IN denotes of
T462 875-885 NN denotes expression
T461 871-874 CC denotes and
T460 865-870 NNP denotes NF-κB
T459 862-864 IN denotes of
T458 853-861 NN denotes activity
T457 849-852 DT denotes The
T456 847-848 . denotes .
T455 843-847 NNP denotes MMP9
T454 840-842 IN denotes of
T453 831-839 NN denotes activity
T452 827-830 DT denotes the
T451 820-826 VB denotes detect
T450 817-819 TO denotes to
T449 807-816 VBN denotes performed
T448 803-806 VBD denotes was
T447 792-802 NN denotes zymography
T446 784-791 NN denotes Gelatin
T445 782-783 . denotes .
T444 773-782 VBN denotes evaluated
T443 768-772 RB denotes also
T442 763-767 VBD denotes were
T441 758-762 NNP denotes MMP9
T440 754-757 CC denotes and
T439 752-753 , denotes ,
T438 748-752 NNP denotes IL-6
T437 746-747 , denotes ,
T436 741-746 NNP denotes IL-1β
T435 739-740 , denotes ,
T434 734-739 NNP denotes TNF-α
T433 731-733 IN denotes of
T432 724-730 NNS denotes levels
T431 716-723 NN denotes protein
T430 712-715 CC denotes and
T429 707-711 NNP denotes mRNA
T428 703-706 DT denotes The
T427 701-702 . denotes .
T426 697-701 NNP denotes EMSA
T425 694-696 IN denotes by
T424 684-693 VBN denotes evaluated
T423 680-683 VBD denotes was
T422 674-679 NN denotes NF-κB
T421 671-673 IN denotes of
T420 662-670 NN denotes activity
T419 650-661 JJ denotes DNA-binding
T418 646-649 DT denotes the
T417 641-645 RB denotes Then
T416 639-640 . denotes .
T415 634-639 NN denotes vitro
T414 631-633 IN denotes in
T413 624-630 NN denotes injury
T412 614-623 JJ denotes traumatic
T411 610-613 DT denotes the
T410 604-609 VB denotes mimic
T409 601-603 TO denotes to
T408 591-600 VBN denotes scratched
T407 586-590 VBD denotes were
T406 575-585 NNS denotes astrocytes
T405 566-574 JJ denotes cultured
T404 558-565 JJ denotes Primary
T403 556-557 . denotes .
T402 551-556 NN denotes vitro
T401 548-550 IN denotes in
T400 544-547 NNP denotes TBI
T399 541-543 IN denotes of
T398 535-540 NN denotes model
T397 533-534 DT denotes a
T396 530-532 IN denotes in
T395 520-529 NNS denotes cytokines
T394 504-519 JJ denotes proinflammatory
T393 501-503 IN denotes of
T392 490-500 NN denotes expression
T391 486-489 CC denotes and
T390 480-485 NNP denotes NF-κB
T389 477-479 IN denotes of
T388 466-476 NN denotes activation
T387 462-465 DT denotes the
T386 459-461 IN denotes on
T385 454-458 NNP denotes Nrf2
T384 451-453 IN denotes of
T383 441-450 NN denotes influence
T382 437-440 DT denotes the
T381 431-436 VB denotes study
T380 428-430 TO denotes to
T379 423-427 NNS denotes mice
T378 414-422 NN denotes knockout
T377 411-413 CC denotes or
T376 402-410 NN denotes wildtype
T375 397-401 JJ denotes Nrf2
T374 390-396 DT denotes either
T373 385-389 IN denotes from
T372 374-384 NNS denotes astrocytes
T371 365-373 JJ denotes cultured
T370 357-364 JJ denotes primary
T369 352-356 VBD denotes used
T368 349-351 PRP denotes we
T367 347-348 , denotes ,
T366 342-347 RB denotes Hence
T365 340-341 . denotes .
T364 337-340 NNP denotes TBI
T363 331-336 IN denotes after
T362 320-330 NNS denotes astrocytes
T361 317-319 IN denotes in
T360 311-316 NNP denotes NF-κB
T359 307-310 CC denotes and
T358 302-306 NNP denotes Nrf2
T357 294-301 IN denotes between
T356 281-293 NN denotes relationship
T355 277-280 DT denotes the
T354 271-276 IN denotes about
T353 265-270 VBN denotes known
T352 262-264 VBZ denotes is
T351 255-261 RB denotes little
T350 251-254 CC denotes but
T349 249-250 , denotes ,
T348 246-249 NNP denotes TBI
T347 240-245 IN denotes after
T346 234-239 NN denotes brain
T345 230-233 DT denotes the
T344 227-229 IN denotes in
T343 221-226 NN denotes NF-κB
T342 218-220 IN denotes of
T341 207-217 NN denotes activation
T340 199-206 IN denotes through
T339 191-198 NN denotes process
T338 178-190 JJ denotes inflammatory
T337 169-177 VBZ denotes enhances
T1032 1970-1974 WDT denotes that
T1031 1963-1969 NN denotes factor
T1030 1949-1962 NN denotes transcription
T1029 1947-1948 DT denotes a
T1028 1944-1946 VBZ denotes is
T1027 1942-1943 -RRB- denotes )
T1026 1938-1942 NN denotes Nrf2
T1025 1937-1938 -LRB- denotes (
T1024 1929-1936 CD denotes factor2
T1023 1919-1928 JJ denotes 2-related
T1022 1909-1918 VBD denotes erythroid
T1021 1902-1908 NN denotes factor
T1020 1894-1901 NNP denotes Nuclear
T1019 1892-1893 . denotes .
T1018 1891-1892 NNP denotes ]
T1017 1890-1891 CD denotes 4
T1016 1888-1889 CD denotes 2
T1015 1887-1888 VBP denotes [
T1014 1880-1886 NNS denotes organs
T1013 1874-1879 JJ denotes other
T1012 1871-1873 IN denotes in
T982 1695-1698 DT denotes the
T981 1692-1694 IN denotes as
T980 1687-1691 RB denotes well
T979 1684-1686 RB denotes as
T978 1682-1683 , denotes ,
T977 1677-1682 NNS denotes cells
T976 1661-1676 JJ denotes immunocompetent
T975 1652-1660 NN denotes resident
T974 1649-1651 IN denotes of
T973 1638-1648 NN denotes activation
T972 1634-1637 CC denotes and
T971 1632-1633 , denotes ,
T970 1627-1632 NNS denotes cells
T969 1621-1626 NN denotes blood
T968 1610-1620 JJ denotes peripheral
T967 1607-1609 IN denotes of
T966 1594-1606 NN denotes infiltration
T965 1592-1593 , denotes ,
T964 1587-1592 NN denotes edema
T963 1581-1586 NN denotes brain
T962 1579-1580 , denotes ,
T961 1578-1579 -RRB- denotes )
T960 1575-1578 NNP denotes BBB
T959 1574-1575 -LRB- denotes (
T958 1566-1573 NN denotes barrier
T957 1554-1565 JJ denotes blood-brain
T956 1551-1553 IN denotes of
T955 1541-1550 NN denotes breakdown
T954 1531-1540 VBG denotes including
T953 1529-1530 , denotes ,
T952 1521-1529 NN denotes response
T951 1508-1520 JJ denotes inflammatory
T950 1502-1507 JJ denotes acute
T949 1499-1501 DT denotes an
T948 1496-1498 IN denotes of
T947 1485-1495 NN denotes initiation
T946 1481-1484 DT denotes the
T945 1472-1480 VBZ denotes involves
T944 1462-1471 NN denotes mechanism
T943 1455-1461 NN denotes injury
T942 1445-1454 JJ denotes secondary
T941 1441-1444 DT denotes The
T940 1439-1440 . denotes .
T939 1429-1439 NNS denotes mechanisms
T938 1427-1428 -RRB- denotes )
T937 1421-1427 NN denotes injury
T936 1413-1420 JJ denotes delayed
T935 1410-1412 CC denotes or
T934 1400-1409 JJ denotes secondary
T933 1399-1400 -LRB- denotes (
T932 1390-1398 JJ denotes indirect
T931 1386-1389 CC denotes and
T336 159-168 NN denotes depletion
T335 154-158 JJ denotes Nrf2
T334 149-153 IN denotes that
T333 142-148 VBN denotes proved
T332 137-141 VBN denotes been
T331 133-136 VBZ denotes has
T330 130-132 PRP denotes It
T1011 1866-1870 RB denotes also
T1010 1862-1865 CC denotes but
T1009 1856-1861 NN denotes brain
T1008 1853-1855 IN denotes in
T1007 1848-1852 RB denotes only
T1006 1844-1847 RB denotes not
T1005 1837-1843 NN denotes damage
T1004 1827-1836 JJ denotes secondary
T1003 1824-1826 TO denotes to
T1002 1818-1823 VBZ denotes leads
T1001 1805-1817 NN denotes inflammation
T1000 1794-1804 JJ denotes subsequent
T999 1790-1793 DT denotes the
T998 1786-1789 CC denotes And
T997 1784-1785 . denotes .
T996 1783-1784 NNP denotes ]
T995 1782-1783 CD denotes 1
T994 1781-1782 VBP denotes [
T993 1773-1780 NNS denotes factors
T992 1761-1772 JJ denotes chemotactic
T991 1757-1760 CC denotes and
T990 1744-1756 NNS denotes interleukins
T989 1741-1743 IN denotes as
T988 1736-1740 JJ denotes such
T987 1726-1735 NNS denotes mediators
T986 1719-1725 JJ denotes immune
T985 1710-1718 JJ denotes numerous
T984 1707-1709 IN denotes of
T983 1699-1706 NN denotes release
T4980 11710-11714 NNP denotes Cell
T4979 11706-11709 DT denotes the
T4978 11695-11705 NNP denotes Aggravated
T4977 11690-11694 NNP denotes Nrf2
T4976 11687-11689 IN denotes of
T4975 11677-11686 NN denotes Depletion
T4974 11675-11676 . denotes .
T4973 11672-11675 CD denotes 3.1
T4996 11813-11815 IN denotes at
T4995 11804-11812 NN denotes analysis
T4994 11798-11803 NN denotes CCK-8
T4993 11794-11797 CC denotes and
T4992 11783-11793 NN denotes microscope
T4991 11780-11782 IN denotes by
T4990 11771-11779 VBN denotes detected
T4989 11767-11770 VBD denotes was
T4988 11761-11766 NN denotes death
T4987 11756-11760 NNP denotes Cell
T4986 11749-11755 NNP denotes Injury
T4985 11741-11748 NNP denotes Scratch
T4984 11735-11740 IN denotes after
T4983 11724-11734 NNP denotes Astrocytes
T4982 11721-11723 IN denotes in
T4981 11715-11720 NNP denotes Death
T5027 11980-11983 NNP denotes TBI
T5026 11977-11979 NNP denotes KO
T5061 12106-12107 , denotes ,
T5060 12105-12106 -RRB- denotes )
T5059 12101-12105 CD denotes 0.01
T5058 12099-12100 CD denotes <
T5057 12097-12098 NNP denotes P
T5056 12096-12097 -LRB- denotes (
T5055 12094-12095 -RRB- denotes )
T5054 12089-12094 CD denotes 0.098
T5053 12087-12088 CD denotes ±
T5052 12082-12086 CD denotes 0.98
T5051 12081-12082 -LRB- denotes (
T5050 12077-12080 NNP denotes TBI
T5049 12074-12076 NNP denotes WT
T5048 12068-12073 NN denotes group
T5047 12065-12067 IN denotes of
T5046 12060-12064 DT denotes that
T5045 12055-12059 IN denotes than
T5044 12053-12054 -RRB- denotes )
T5043 12048-12053 CD denotes 0.064
T5042 12046-12047 CD denotes ±
T5041 12041-12045 CD denotes 0.38
T5040 12040-12041 -LRB- denotes (
T5039 12036-12039 NNP denotes TBI
T5038 12033-12035 NNP denotes KO
T5037 12027-12032 NN denotes group
T5036 12024-12026 IN denotes of
T5035 12018-12023 NN denotes value
T5034 12015-12017 NNP denotes OD
T5033 12009-12014 JJR denotes lower
T5032 12002-12008 VBD denotes showed
T5031 11997-12001 RB denotes also
T5030 11991-11996 NN denotes assay
T5029 11985-11990 CD denotes CCK-8
T5028 11983-11984 . denotes .
T3800 8336-8338 IN denotes in
T3799 8321-8335 JJ denotes heat-denatured
T3798 8316-8320 VBD denotes were
T3797 8307-8315 NNS denotes extracts
T3796 8299-8306 NN denotes protein
T3795 8289-8298 JJ denotes cytosolic
T3794 8279-8288 VBG denotes resulting
T3793 8275-8278 DT denotes the
T3792 8272-8274 IN denotes of
T3791 8261-8271 NNS denotes micrograms
T3790 8255-8260 NNP denotes Fifty
T3789 8253-8254 . denotes .
T3788 8252-8253 -RRB- denotes )
T8853 16086-16091 NNP denotes TNF-α
T8852 16076-16085 VBG denotes including
T8851 16074-16075 , denotes ,
T8850 16065-16074 NNS denotes cytokines
T1522 2974-2982 VBN denotes provided
T1509 2884-2891 JJ denotes Primary
T1508 2882-2883 . denotes .
T1507 2879-2882 CD denotes 2.1
T1521 2967-2973 RB denotes kindly
T1520 2962-2966 VBD denotes were
T1519 2957-2961 NNS denotes mice
T1518 2953-2956 NNP denotes ICR
T1517 2944-2952 NN denotes knockout
T1516 2939-2943 NNP denotes Nrf2
T1515 2928-2938 NNP denotes Astrocytes
T1514 2922-2927 NNP denotes Mouse
T1513 2919-2921 IN denotes of
T1512 2904-2918 NNP denotes Identification
T1511 2900-2903 CC denotes and
T1510 2892-2899 NNP denotes Culture
T1590 3325-3332 VBN denotes chopped
T1589 3317-3324 RB denotes roughly
T1588 3313-3316 VBD denotes was
T1587 3306-3312 NN denotes tissue
T1586 3302-3305 DT denotes The
T1585 3300-3301 . denotes .
T1584 3293-3300 VBN denotes removed
T1583 3288-3292 VBD denotes were
T1582 3280-3287 NNS denotes vessels
T1581 3274-3279 NN denotes blood
T1580 3263-3273 VBN denotes associated
T1579 3259-3262 CC denotes and
T1578 3250-3258 NNS denotes meninges
T1577 3246-3249 DT denotes the
T1576 3242-3245 CC denotes and
T1575 3240-3241 , denotes ,
T8849 16049-16064 JJ denotes proinflammatory
T3807 8380-8382 IN denotes by
T3806 8370-8379 VBN denotes separated
T3805 8368-8369 , denotes ,
T3804 8362-8368 NN denotes buffer
T3803 8354-8361 VBG denotes loading
T3802 8347-8353 NN denotes sample
T3801 8339-8346 NNP denotes Laemmli
T8848 16046-16048 IN denotes of
T8847 16038-16045 NN denotes battery
T8846 16036-16037 DT denotes a
T3890 8808-8815 JJ denotes primary
T3889 8803-8807 DT denotes Each
T3888 8801-8802 . denotes .
T3887 8800-8801 -RRB- denotes )
T3886 8797-8800 NN denotes kDa
T3885 8794-8796 CD denotes 92
T3884 8792-8793 , denotes ,
T3883 8789-8792 NNP denotes USA
T3882 8787-8788 , denotes ,
T3881 8785-8787 NNP denotes CA
T3880 8783-8784 , denotes ,
T3879 8770-8783 NNP denotes Biotechnology
T3878 8765-8769 NNP denotes Cruz
T3877 8759-8764 NNP denotes Santa
T3876 8757-8758 , denotes ,
T3875 8750-8757 JJ denotes sc-6841
T3874 8749-8750 -LRB- denotes (
T3873 8747-8748 -RRB- denotes )
T3872 8743-8747 NNP denotes MMP9
T3871 8742-8743 -LRB- denotes (
T3870 8740-8741 CD denotes 9
T3869 8723-8739 NN denotes metallopeptidase
T3868 8711-8722 JJ denotes anti-matrix
T3867 8707-8710 CC denotes and
T3866 8705-8706 -RRB- denotes )
T8845 16032-16035 CC denotes and
T8844 16027-16031 NNP denotes MMP9
T4681 11161-11162 , denotes ,
T4680 11156-11161 NN denotes water
T4679 11153-11155 IN denotes in
T4678 11145-11152 VBG denotes rinsing
T4677 11139-11144 IN denotes After
T4676 11137-11138 . denotes .
T4675 11130-11137 NN denotes gelatin
T4674 11126-11129 DT denotes the
T4673 11119-11125 VB denotes cleave
T4672 11116-11118 TO denotes to
T4671 11111-11115 NNP denotes MMP9
T4670 11107-11110 DT denotes the
T4669 11100-11106 VB denotes enable
T4668 11097-11099 TO denotes to
T4667 11095-11096 NNP denotes C
T4666 11094-11095 CD denotes °
T4665 11092-11094 CD denotes 37
T4664 11089-11091 IN denotes at
T4663 11087-11088 NN denotes h
T4662 11084-11086 CD denotes 40
T4661 11080-11083 IN denotes for
T4660 11073-11079 NN denotes buffer
T4659 11063-11072 JJ denotes substrate
T4658 11060-11062 IN denotes in
T4657 11050-11059 VBD denotes incubated
T4656 11045-11049 RB denotes then
T4655 11041-11044 CC denotes and
T4654 11035-11040 NNP denotes X-100
T4653 11028-11034 NNP denotes Triton
T4724 11399-11403 IN denotes with
T8843 16024-16026 IN denotes of
T8842 16010-16023 NN denotes transcription
T8841 16002-16009 VBZ denotes induces
T8840 15998-16001 CC denotes and
T8839 15988-15997 VBN denotes activated
T8838 15984-15987 VBD denotes was
T8837 15981-15983 PRP denotes it
T8836 15976-15980 IN denotes once
T8835 15972-15975 NNP denotes DNA
T8834 15967-15971 IN denotes with
T8833 15961-15966 NNS denotes binds
T8862 16119-16126 NNS denotes tissues
T8861 16113-16118 NN denotes brain
T8860 16110-16112 IN denotes in
T8859 16108-16109 , denotes ,
T8858 16104-16108 NNP denotes IL-6
T8857 16100-16103 CC denotes and
T8856 16098-16099 , denotes ,
T8855 16093-16098 NNP denotes IL-1β
T8854 16091-16092 , denotes ,
T9411 19238-19241 DT denotes the
T6318 14462-14463 . denotes .
T6317 14461-14462 -RRB- denotes )
T6316 14460-14461 -RRB- denotes )
T6315 14459-14460 NN denotes c
T6314 14458-14459 -LRB- denotes (
T6313 14457-14458 CD denotes 4
T6312 14450-14456 NN denotes Figure
T6311 14449-14450 -LRB- denotes (
T6310 14447-14448 -RRB- denotes )
T6309 14443-14447 CD denotes 0.01
T6308 14441-14442 CD denotes <
T6307 14439-14440 NNP denotes P
T6306 14438-14439 -LRB- denotes (
T6305 14434-14437 NNP denotes TBI
T6304 14431-14433 NNP denotes WT
T6303 14425-14430 NN denotes group
T6302 14421-14424 IN denotes for
T6301 14415-14420 CD denotes 5.348
T6300 14413-14414 CD denotes ±
T6299 14406-14412 CD denotes 154.50
T930 1384-1385 -RRB- denotes )
T929 1378-1384 NN denotes injury
T928 1370-1377 JJ denotes primary
T927 1369-1370 -LRB- denotes (
T926 1362-1368 JJ denotes direct
T925 1359-1361 IN denotes of
T924 1352-1358 NN denotes result
T923 1350-1351 DT denotes a
T922 1347-1349 VBZ denotes is
T921 1340-1346 NN denotes injury
T920 1330-1339 JJ denotes traumatic
T919 1320-1329 VBG denotes following
T918 1313-1319 NN denotes damage
T917 1307-1312 NN denotes Brain
T6915 15237-15238 -LRB- denotes (
T6914 15233-15236 NNP denotes TBI
T6913 15230-15232 NNP denotes WT
T6912 15224-15229 NN denotes group
T6911 15220-15223 IN denotes for
T6910 15214-15219 CD denotes 0.048
T6909 15212-15213 CD denotes ±
T6908 15207-15211 CD denotes 1.13
T6907 15203-15206 CC denotes and
T6906 15199-15202 NNP denotes TBI
T6885 15086-15087 . denotes .
T6884 15085-15086 -RRB- denotes )
T6883 15084-15085 -RRB- denotes )
T6882 15083-15084 LS denotes e
T6881 15082-15083 -LRB- denotes (
T6880 15081-15082 CD denotes 3
T6879 15077-15080 CC denotes and
T6878 15075-15076 -RRB- denotes )
T6877 15074-15075 DT denotes a
T6876 15073-15074 -LRB- denotes (
T6875 15072-15073 CD denotes 3
T6874 15064-15071 NNS denotes Figures
T6873 15063-15064 -LRB- denotes (
T6872 15061-15062 -RRB- denotes )
T6905 15196-15198 NNP denotes KO
T6904 15190-15195 NN denotes group
T6903 15186-15189 IN denotes for
T6902 15180-15185 CD denotes 0.076
T6901 15178-15179 CD denotes ±
T6900 15173-15177 CD denotes 1.44
T6899 15170-15172 IN denotes as
T6898 15168-15169 , denotes ,
T6897 15164-15168 NN denotes blot
T6896 15156-15163 JJ denotes western
T6895 15153-15155 IN denotes by
T6894 15147-15152 NN denotes level
T6893 15139-15146 NN denotes protein
T6892 15134-15138 CD denotes MMP9
T6891 15131-15133 IN denotes in
T6890 15120-15130 VBN denotes discovered
T6889 15115-15119 RB denotes also
T6888 15111-15114 VBD denotes was
T6887 15100-15110 NN denotes difference
T6886 15088-15099 JJ denotes Significant
T6952 15396-15397 VBD denotes <
T6951 15394-15395 NNP denotes P
T6950 15392-15393 , denotes ,
T6949 15387-15392 CD denotes 3.388
T6948 15385-15386 CD denotes ±
T6947 15379-15384 CD denotes 76.32
T6946 15372-15378 IN denotes versus
T6945 15366-15371 CD denotes 6.343
T6944 15364-15365 CD denotes ±
T6943 15357-15363 CD denotes 101.76
T6942 15356-15357 -LRB- denotes (
T6941 15352-15355 RB denotes too
T6940 15348-15351 NNP denotes TBI
T6939 15345-15347 NNP denotes WT
T6938 15339-15344 NN denotes group
T6937 15336-15338 IN denotes in
T6936 15331-15335 DT denotes that
T6935 15326-15330 IN denotes than
T6934 15322-15325 NNP denotes TBI
T6933 15319-15321 NNP denotes KO
T6932 15313-15318 NN denotes group
T6931 15310-15312 IN denotes in
T6930 15301-15309 NN denotes activity
T6929 15296-15300 JJ denotes MMP9
T6928 15289-15295 JJR denotes higher
T6927 15280-15288 VBD denotes revealed
T6926 15269-15279 NN denotes zymography
T6925 15260-15268 JJ denotes Gelatine
T6924 15258-15259 . denotes .
T6923 15257-15258 -RRB- denotes )
T6922 15256-15257 CD denotes 5
T6921 15249-15255 NN denotes Figure
T6920 15248-15249 -LRB- denotes (
T6919 15246-15247 -RRB- denotes )
T6918 15242-15246 CD denotes 0.01
T6917 15240-15241 CD denotes <
T6916 15238-15239 NNP denotes P
T5236 12287-12298 NNP denotes DNA-Binding
T5235 12281-12286 NNP denotes NF-κB
T5234 12278-12280 IN denotes of
T5233 12265-12277 NNP denotes Upregulation
T5232 12256-12264 NNP denotes Enhanced
T5231 12251-12255 NNP denotes Nrf2
T5230 12248-12250 IN denotes of
T5229 12237-12247 NN denotes Disruption
T5228 12235-12236 . denotes .
T5227 12232-12235 CD denotes 3.2
T2255 5259-5263 VBN denotes read
T2254 5255-5258 VBD denotes was
R247 T345 T346 det the,brain
R248 T346 T344 pobj brain,in
R249 T347 T337 prep after,enhances
R250 T348 T347 pobj TBI,after
R251 T349 T337 punct ",",enhances
R252 T350 T337 cc but,enhances
R253 T351 T353 advmod little,known
R254 T352 T353 auxpass is,known
R255 T353 T333 conj known,proved
R256 T354 T353 prep about,known
R257 T355 T356 det the,relationship
R258 T356 T354 pobj relationship,about
R259 T357 T356 prep between,relationship
R260 T358 T357 pobj Nrf2,between
R261 T359 T358 cc and,Nrf2
R262 T360 T358 conj NF-κB,Nrf2
R263 T361 T356 prep in,relationship
R264 T362 T361 pobj astrocytes,in
R265 T363 T356 prep after,relationship
R266 T364 T363 pobj TBI,after
R267 T365 T333 punct .,proved
R268 T366 T369 advmod Hence,used
R269 T367 T369 punct ",",used
R270 T368 T369 nsubj we,used
R271 T369 T369 ROOT used,used
R272 T370 T372 amod primary,astrocytes
R273 T371 T372 amod cultured,astrocytes
R274 T372 T369 dobj astrocytes,used
R275 T373 T372 prep from,astrocytes
R276 T374 T376 preconj either,wildtype
R277 T375 T376 amod Nrf2,wildtype
R278 T376 T373 pobj wildtype,from
R279 T377 T376 cc or,wildtype
R280 T378 T379 compound knockout,mice
R281 T379 T376 conj mice,wildtype
R282 T380 T381 aux to,study
R283 T381 T369 xcomp study,used
R284 T382 T383 det the,influence
R285 T383 T381 dobj influence,study
R286 T384 T383 prep of,influence
R287 T385 T384 pobj Nrf2,of
R288 T386 T383 prep on,influence
R289 T387 T388 det the,activation
R290 T388 T386 pobj activation,on
R291 T389 T388 prep of,activation
R292 T390 T389 pobj NF-κB,of
R293 T391 T388 cc and,activation
R294 T392 T388 conj expression,activation
R295 T393 T388 prep of,activation
R296 T394 T395 amod proinflammatory,cytokines
R297 T395 T393 pobj cytokines,of
R298 T396 T388 prep in,activation
R299 T397 T398 det a,model
R300 T398 T396 pobj model,in
R301 T399 T398 prep of,model
R302 T400 T399 pobj TBI,of
R303 T401 T388 prep in,activation
R304 T402 T401 pobj vitro,in
R305 T403 T369 punct .,used
R306 T404 T406 amod Primary,astrocytes
R307 T405 T406 amod cultured,astrocytes
R308 T406 T408 nsubjpass astrocytes,scratched
R309 T407 T408 auxpass were,scratched
R310 T408 T408 ROOT scratched,scratched
R311 T409 T410 aux to,mimic
R312 T410 T408 advcl mimic,scratched
R313 T411 T413 det the,injury
R314 T412 T413 amod traumatic,injury
R315 T413 T410 dobj injury,mimic
R316 T414 T413 prep in,injury
R317 T415 T414 pobj vitro,in
R318 T416 T408 punct .,scratched
R319 T417 T424 advmod Then,evaluated
R320 T418 T420 det the,activity
R321 T419 T420 amod DNA-binding,activity
R322 T420 T424 nsubjpass activity,evaluated
R323 T421 T420 prep of,activity
R324 T422 T421 pobj NF-κB,of
R325 T423 T424 auxpass was,evaluated
R326 T424 T424 ROOT evaluated,evaluated
R327 T425 T424 agent by,evaluated
R328 T426 T425 pobj EMSA,by
R329 T427 T424 punct .,evaluated
R330 T428 T429 det The,mRNA
R331 T429 T444 nsubjpass mRNA,evaluated
R332 T430 T429 cc and,mRNA
R333 T431 T432 compound protein,levels
R334 T432 T429 conj levels,mRNA
R335 T433 T432 prep of,levels
R349 T447 T449 nsubjpass zymography,performed
R350 T448 T449 auxpass was,performed
R351 T449 T449 ROOT performed,performed
R352 T450 T451 aux to,detect
R353 T451 T449 advcl detect,performed
R354 T452 T453 det the,activity
R355 T453 T451 dobj activity,detect
R356 T454 T453 prep of,activity
R357 T455 T454 pobj MMP9,of
R358 T456 T449 punct .,performed
R359 T457 T458 det The,activity
R360 T458 T469 nsubjpass activity,upregulated
R361 T459 T458 prep of,activity
R362 T460 T459 pobj NF-κB,of
R363 T461 T458 cc and,activity
R364 T462 T458 conj expression,activity
R365 T463 T462 prep of,expression
R366 T464 T465 amod proinflammatory,cytokines
R367 T465 T463 pobj cytokines,of
R368 T466 T465 acl mentioned,cytokines
R369 T467 T466 advmod above,mentioned
R370 T468 T469 auxpass were,upregulated
R371 T469 T469 ROOT upregulated,upregulated
R372 T470 T469 prep at,upregulated
R373 T471 T472 nummod 24,h
R374 T472 T470 pobj h,at
R375 T473 T469 prep after,upregulated
R376 T474 T473 pobj scratch,after
R377 T475 T469 punct .,upregulated
R378 T476 T477 det The,expression
R379 T477 T484 nsubjpass expression,elevated
R380 T478 T477 cc and,expression
R381 T479 T477 conj activity,expression
R382 T480 T477 prep of,expression
R383 T481 T480 pobj MMP9,of
R384 T482 T484 auxpass were,elevated
R385 T483 T484 advmod also,elevated
R386 T484 T484 ROOT elevated,elevated
R387 T485 T484 punct .,elevated
R388 T486 T489 cc And,was
R389 T487 T488 amod such,tendency
R390 T488 T489 nsubj tendency,was
R391 T489 T489 ROOT was,was
R392 T490 T491 advmod much,more
R393 T491 T492 advmod more,prominent
R394 T492 T489 acomp prominent,was
R395 T493 T492 prep in,prominent
R396 T494 T495 compound Nrf2,KO
R397 T495 T496 compound KO,astrocytes
R398 T496 T493 pobj astrocytes,in
R399 T497 T492 prep than,prominent
R400 T498 T497 pobj that,than
R401 T499 T498 prep in,that
R714 T922 T922 ROOT is,is
R715 T923 T924 det a,result
R716 T924 T922 attr result,is
R717 T925 T924 prep of,result
R718 T926 T929 amod direct,injury
R719 T927 T929 punct (,injury
R720 T928 T929 amod primary,injury
R721 T929 T939 nmod injury,mechanisms
R722 T930 T929 punct ),injury
R723 T931 T929 cc and,injury
R724 T932 T937 amod indirect,injury
R725 T933 T937 punct (,injury
R726 T934 T937 amod secondary,injury
R727 T935 T934 cc or,secondary
R728 T936 T934 conj delayed,secondary
R729 T937 T929 conj injury,injury
R730 T938 T937 punct ),injury
R731 T939 T925 pobj mechanisms,of
R732 T940 T922 punct .,is
R733 T941 T944 det The,mechanism
R734 T942 T944 amod secondary,mechanism
R735 T943 T944 compound injury,mechanism
R736 T944 T945 nsubj mechanism,involves
R737 T945 T945 ROOT involves,involves
R738 T946 T947 det the,initiation
R739 T947 T945 dobj initiation,involves
R740 T948 T947 prep of,initiation
R741 T949 T952 det an,response
R742 T950 T952 amod acute,response
R743 T951 T952 amod inflammatory,response
R744 T952 T948 pobj response,of
R745 T953 T947 punct ",",initiation
R746 T954 T947 prep including,initiation
R747 T955 T954 pobj breakdown,including
R748 T956 T955 prep of,breakdown
R749 T957 T958 amod blood-brain,barrier
R750 T958 T956 pobj barrier,of
R751 T959 T960 punct (,BBB
R752 T960 T955 parataxis BBB,breakdown
R753 T961 T960 punct ),BBB
R754 T962 T955 punct ",",breakdown
R755 T963 T964 compound brain,edema
R756 T964 T955 conj edema,breakdown
R757 T965 T964 punct ",",edema
R758 T966 T964 conj infiltration,edema
R759 T967 T966 prep of,infiltration
R760 T968 T970 amod peripheral,cells
R761 T969 T970 compound blood,cells
R762 T970 T967 pobj cells,of
R763 T971 T966 punct ",",infiltration
R764 T972 T966 cc and,infiltration
R765 T973 T966 conj activation,infiltration
R766 T974 T973 prep of,activation
R767 T975 T977 amod resident,cells
R768 T976 T977 amod immunocompetent,cells
R769 T977 T974 pobj cells,of
R770 T978 T966 punct ",",infiltration
R771 T979 T981 advmod as,as
R772 T980 T981 advmod well,as
R773 T981 T955 cc as,breakdown
R774 T982 T983 det the,release
R775 T983 T947 conj release,initiation
R776 T984 T983 prep of,release
R777 T985 T987 amod numerous,mediators
R778 T986 T987 amod immune,mediators
R779 T987 T984 pobj mediators,of
R780 T988 T989 amod such,as
R781 T989 T987 prep as,mediators
R782 T990 T989 pobj interleukins,as
R783 T991 T990 cc and,interleukins
R784 T992 T993 amod chemotactic,factors
R785 T993 T990 conj factors,interleukins
R786 T994 T945 conj [,involves
R787 T995 T996 nummod 1,]
R788 T996 T994 dobj ],[
R789 T997 T945 punct .,involves
R790 T998 T1002 cc And,leads
R791 T999 T1001 det the,inflammation
R792 T1000 T1001 amod subsequent,inflammation
R793 T1001 T1002 nsubj inflammation,leads
R794 T1002 T1002 ROOT leads,leads
R795 T1003 T1002 prep to,leads
R796 T1004 T1005 amod secondary,damage
R797 T1005 T1003 pobj damage,to
R798 T1006 T1008 preconj not,in
R799 T1007 T1006 advmod only,not
R800 T1008 T1005 prep in,damage
R801 T1009 T1008 pobj brain,in
R802 T1010 T1008 cc but,in
R803 T1011 T1010 advmod also,but
R804 T1012 T1008 conj in,in
R805 T1013 T1014 amod other,organs
R806 T1014 T1012 pobj organs,in
R807 T1015 T1008 pobj [,in
R808 T1016 T1015 nummod 2,[
R809 T1017 T1018 nummod 4,]
R810 T1018 T1015 appos ],[
R811 T1019 T1002 punct .,leads
R812 T1020 T1021 compound Nuclear,factor
R813 T1021 T1022 nsubj factor,erythroid
R814 T1022 T1028 nsubj erythroid,is
R815 T1023 T1022 advmod 2-related,erythroid
R816 T1024 T1023 nummod factor2,2-related
R817 T1025 T1026 punct (,Nrf2
R818 T1026 T1023 appos Nrf2,2-related
R819 T1027 T1022 punct ),erythroid
R820 T1028 T1028 ROOT is,is
R821 T1029 T1031 det a,factor
R822 T1030 T1031 compound transcription,factor
R823 T1031 T1028 attr factor,is
R824 T1032 T1033 nsubj that,regulates
R825 T1033 T1031 relcl regulates,factor
R826 T1034 T1035 amod many,kinds
R827 T1035 T1033 dobj kinds,regulates
R828 T1036 T1035 prep of,kinds
R829 T1037 T1038 amod antioxidant,genes
R830 T1038 T1036 pobj genes,of
R831 T1039 T1028 punct .,is
R832 T1040 T1041 amod Several,studies
R833 T1041 T1043 nsubj studies,demonstrated
R834 T1042 T1043 aux have,demonstrated
R835 T1043 T1043 ROOT demonstrated,demonstrated
R836 T1044 T1046 mark that,regulates
R837 T1045 T1046 nsubj Nrf2,regulates
R838 T1046 T1043 ccomp regulates,demonstrated
R839 T1047 T1048 det the,inflammation
R840 T1048 T1046 dobj inflammation,regulates
R841 T1049 T1048 prep in,inflammation
R842 T1050 T1051 det the,brain
R843 T1051 T1049 pobj brain,in
R844 T1052 T1046 prep after,regulates
R845 T1053 T1055 amod traumatic,injury
R846 T1054 T1055 compound brain,injury
R847 T1055 T1052 pobj injury,after
R848 T1056 T1055 punct (,injury
R849 T1057 T1055 appos TBI,injury
R850 T1058 T1055 punct ),injury
R851 T1059 T1043 punct .,demonstrated
R852 T1060 T1063 nsubjpass It,showed
R853 T1061 T1063 auxpass was,showed
R854 T1062 T1063 advmod also,showed
R855 T1063 T1063 ROOT showed,showed
R856 T1064 T1067 mark that,induce
R857 T1065 T1067 nsubj TBI,induce
R858 T1066 T1067 aux could,induce
R859 T1067 T1063 ccomp induce,showed
R860 T1068 T1069 advmod more,aggravated
R861 T1069 T1070 amod aggravated,damage
R862 T1070 T1067 dobj damage,induce
R863 T1071 T1070 prep in,damage
R864 T1072 T1074 amod Nrf2,mice
R865 T1073 T1074 compound knockout,mice
R866 T1074 T1071 pobj mice,in
R867 T1075 T1070 prep than,damage
R868 T1076 T1075 prep in,than
R869 T1077 T1078 compound wildtype,mice
R870 T1078 T1076 pobj mice,in
R871 T1079 T1075 pobj [,than
R872 T1080 T1081 nummod 5,]
R873 T1081 T1079 nsubj ],[
R874 T1082 T1063 punct .,showed
R875 T1083 T1091 prep On,extenuated
R876 T1084 T1085 det the,contrary
R877 T1085 T1083 pobj contrary,On
R878 T1086 T1091 punct ",",extenuated
R879 T1087 T1088 amod such,inflammation
R880 T1088 T1091 nsubjpass inflammation,extenuated
R881 T1089 T1091 aux can,extenuated
R882 T1090 T1091 auxpass be,extenuated
R883 T1091 T1091 ROOT extenuated,extenuated
R884 T1092 T1091 prep through,extenuated
R885 T1093 T1092 pcomp elevating,through
R886 T1094 T1095 det the,level
R887 T1095 T1093 dobj level,elevating
R888 T1096 T1095 prep of,level
R889 T1097 T1096 pobj Nrf2,of
R890 T1098 T1093 prep by,elevating
R891 T1099 T1100 poss its,inducers
R892 T1100 T1098 pobj inducers,by
R893 T1101 T1100 punct ",",inducers
R894 T1102 T1103 amod such,as
R895 T1103 T1100 prep as,inducers
R896 T1104 T1103 pobj sulforaphane,as
R897 T1105 T1106 punct (,SFN
R898 T1106 T1108 nmod SFN,[
R899 T1107 T1106 punct ),SFN
R900 T1108 T1110 nmod [,]
R901 T1109 T1110 nummod 6,]
R902 T1110 T1100 appos ],inducers
R903 T1111 T1091 punct .,extenuated
R904 T1112 T1113 nsubj It,is
R905 T1113 T1113 ROOT is,is
R906 T1114 T1115 advmod well,documented
R907 T1115 T1113 acomp documented,is
R908 T1116 T1118 mark that,play
R909 T1117 T1118 nsubj astrocytes,play
R910 T1118 T1115 ccomp play,documented
R911 T1119 T1121 det a,role
R912 T1120 T1121 amod critical,role
R913 T1121 T1118 dobj role,play
R914 T1122 T1121 prep in,role
R915 T1123 T1122 pcomp maintaining,in
R916 T1124 T1126 amod normal,physiology
R917 T1125 T1126 compound brain,physiology
R918 T1126 T1123 dobj physiology,maintaining
R919 T1127 T1123 cc and,maintaining
R920 T1128 T1123 conj responding,maintaining
R921 T1129 T1128 prep to,responding
R922 T1130 T1129 pobj injury,to
R923 T1131 T1130 cc or,injury
R924 T1132 T1130 conj disease,injury
R925 T1133 T1113 punct .,is
R926 T1134 T1142 prep In,performed
R927 T1135 T1134 pobj fact,In
R928 T1136 T1142 punct ",",performed
R929 T1137 T1140 det all,studies
R930 T1138 T1140 det the,studies
R931 T1139 T1140 amod aforementioned,studies
R932 T1140 T1142 nsubjpass studies,performed
R933 T1141 T1142 auxpass are,performed
R934 T1142 T1142 ROOT performed,performed
R935 T1143 T1142 prep in,performed
R936 T1144 T1143 pobj vivo,in
R937 T1145 T1142 punct .,performed
R938 T1146 T1148 nsubjpass Little,known
R939 T1147 T1148 auxpass is,known
R940 T1148 T1148 ROOT known,known
R941 T1149 T1148 prep about,known
R942 T1150 T1151 det the,relationship
R943 T1151 T1149 pobj relationship,about
R944 T1152 T1151 prep between,relationship
R945 T1153 T1152 pobj Nrf2,between
R946 T1154 T1153 cc and,Nrf2
R947 T1155 T1153 conj inflammation,Nrf2
R948 T1156 T1155 prep in,inflammation
R949 T1157 T1156 pobj astrocytes,in
R950 T1158 T1151 prep after,relationship
R951 T1159 T1158 pobj TBI,after
R952 T1160 T1148 punct .,known
R953 T1161 T1165 advmod Therefore,analysed
R954 T1162 T1165 punct ",",analysed
R955 T1163 T1165 advmod here,analysed
R956 T1164 T1165 nsubj we,analysed
R957 T1165 T1165 ROOT analysed,analysed
R958 T1166 T1167 det the,influence
R959 T1167 T1165 dobj influence,analysed
R960 T1168 T1167 prep of,influence
R961 T1169 T1168 pobj Nrf2,of
R962 T1170 T1167 prep on,influence
R963 T1171 T1172 det the,expression
R964 T1172 T1170 pobj expression,on
R965 T1173 T1172 prep of,expression
R966 T1174 T1175 amod proinflammatory,cytokines
R967 T1175 T1173 pobj cytokines,of
R968 T1176 T1175 prep in,cytokines
R969 T1177 T1179 amod primary,astrocytes
R970 T1178 T1179 amod cultured,astrocytes
R971 T1179 T1176 pobj astrocytes,in
R972 T1180 T1179 prep from,astrocytes
R973 T1181 T1182 amod transgenic,mice
R974 T1182 T1180 pobj mice,from
R975 T1183 T1165 prep after,analysed
R976 T1184 T1185 compound scratch,injury
R977 T1185 T1183 pobj injury,after
R978 T1186 T1165 punct .,analysed
R1247 T1507 T1507 ROOT 2.1,2.1
R1248 T1508 T1507 punct .,2.1
R1253 T1513 T1510 prep of,Culture
R1287 T1547 T1549 amod Nrf2,type
R1288 T1548 T1549 amod wide,type
R1289 T1549 T1558 nmod type,mice
R1290 T1550 T1549 punct (,type
R1291 T1551 T1549 appos WT,type
R1292 T1552 T1551 punct ),WT
R1293 T1553 T1549 cc and,type
R1294 T1554 T1549 conj knockout,type
R1295 T1555 T1556 punct (,KO
R1296 T1556 T1554 appos KO,knockout
R1297 T1557 T1554 punct ),knockout
R1298 T1558 T1544 pobj mice,from
R1299 T1559 T1558 punct (,mice
R1310 T1570 T1571 det the,cortices
R1311 T1571 T1573 nsubjpass cortices,dissected
R1312 T1572 T1573 auxpass were,dissected
R1313 T1573 T1573 ROOT dissected,dissected
R1314 T1574 T1573 prt out,dissected
R1315 T1575 T1573 punct ",",dissected
R1316 T1576 T1573 cc and,dissected
R1317 T1577 T1578 det the,meninges
R1318 T1578 T1580 nsubj meninges,associated
R1319 T1579 T1578 cc and,meninges
R1320 T1580 T1582 amod associated,vessels
R1321 T1581 T1582 compound blood,vessels
R1322 T1582 T1584 nsubjpass vessels,removed
R1323 T1583 T1584 auxpass were,removed
R1324 T1584 T1573 conj removed,dissected
R1325 T1585 T1584 punct .,removed
R1326 T1586 T1587 det The,tissue
R1327 T1587 T1590 nsubjpass tissue,chopped
R1328 T1588 T1590 auxpass was,chopped
R1329 T1589 T1590 advmod roughly,chopped
R1330 T1590 T1590 ROOT chopped,chopped
R1331 T1591 T1590 prep with,chopped
R1332 T1592 T1594 det a,blade
R1333 T1593 T1594 compound scalpel,blade
R1334 T1594 T1591 pobj blade,with
R1335 T1595 T1590 punct ",",chopped
R1336 T1596 T1590 conj incubated,chopped
R1337 T1597 T1596 prep in,incubated
R1338 T1598 T1599 nummod 0.5,%
R1339 T1599 T1600 compound %,trypsin
R1340 T1600 T1597 pobj trypsin,in
R1341 T1601 T1596 prep for,incubated
R1342 T1602 T1603 nummod 10,minutes
R1343 T1603 T1601 pobj minutes,for
R1344 T1604 T1596 prep at,incubated
R1345 T1605 T1606 nummod 37,°
R1346 T1606 T1607 nummod °,C
R1347 T1607 T1604 pobj C,at
R1348 T1608 T1596 punct ",",incubated
R1349 T1609 T1596 cc and,incubated
R1350 T1610 T1596 conj agitated,incubated
R1351 T1611 T1613 det every,minutes
R1352 T1612 T1613 amod few,minutes
R1353 T1613 T1590 npadvmod minutes,chopped
R1354 T1614 T1590 punct .,chopped
R1355 T1615 T1621 prep After,rinsed
R1356 T1616 T1615 pobj digestion,After
R1357 T1617 T1621 punct ",",rinsed
R1358 T1618 T1619 det the,tissue
R1359 T1619 T1621 nsubjpass tissue,rinsed
R1360 T1620 T1621 auxpass was,rinsed
R1361 T1621 T1621 ROOT rinsed,rinsed
R1362 T1622 T1621 advmod twice,rinsed
R1363 T1623 T1621 prep in,rinsed
R1364 T1624 T1623 pobj DMEM,in
R1365 T1625 T1621 prep with,rinsed
R1366 T1626 T1627 nummod 10,%
R1367 T1627 T1628 compound %,FBS
R1368 T1628 T1625 pobj FBS,with
R1369 T1629 T1628 punct ",",FBS
R1370 T1630 T1628 acl followed,FBS
R1371 T1631 T1630 agent by,followed
R1372 T1632 T1634 det a,dissociation
R1373 T1633 T1634 amod mechanical,dissociation
R1374 T1634 T1631 pobj dissociation,by
R1375 T1635 T1634 prep in,dissociation
R1376 T1636 T1635 pobj DMEM,in
R1386 T1646 T1647 nummod 5,μg
R1387 T1647 T1648 compound μg,/
R1388 T1648 T1650 compound /,streptomycin
R1389 T1649 T1650 compound mL,streptomycin
R1390 T1650 T1644 appos streptomycin,penicillin
R1391 T1651 T1654 punct (,medium
R1392 T1652 T1654 amod complete,medium
R1393 T1653 T1654 compound culture,medium
R1394 T1654 T1650 appos medium,streptomycin
R1395 T1655 T1650 punct ),streptomycin
R1396 T1656 T1621 punct .,rinsed
R1397 T1657 T1666 prep After,transferred
R1398 T1658 T1657 pobj incubation,After
R1399 T1659 T1658 prep for,incubation
R1400 T1660 T1661 nummod 1,h
R1401 T1661 T1659 pobj h,for
R1402 T1662 T1666 punct ",",transferred
R1403 T1663 T1664 det the,supernatant
R1404 T1664 T1666 nsubjpass supernatant,transferred
R1405 T1665 T1666 auxpass was,transferred
R1406 T1666 T1666 ROOT transferred,transferred
R1407 T1667 T1666 prep to,transferred
R1408 T1668 T1670 det a,flask
R1409 T1669 T1670 amod new,flask
R1410 T1670 T1667 pobj flask,to
R1411 T1671 T1670 cc or,flask
R1412 T1672 T1670 conj dish,flask
R1413 T1673 T1674 punct (,Costar
R1414 T1674 T1670 appos Costar,flask
R1415 T1675 T1674 punct ",",Costar
R1416 T1676 T1674 appos USA,Costar
R1417 T1677 T1674 punct ),Costar
R1418 T1678 T1666 prep for,transferred
R1419 T1679 T1678 pcomp depleting,for
R1420 T1680 T1683 det the,cells
R1421 T1681 T1683 amod residual,cells
R1422 T1682 T1683 amod epithelial,cells
R1423 T1683 T1679 dobj cells,depleting
R1424 T1684 T1666 punct .,transferred
R1425 T1685 T1689 advmod Then,cultivated
R1426 T1686 T1687 det the,cells
R1427 T1687 T1689 nsubjpass cells,cultivated
R1428 T1688 T1689 auxpass were,cultivated
R1429 T1689 T1689 ROOT cultivated,cultivated
R1430 T1690 T1689 prep at,cultivated
R1431 T1691 T1692 nummod 37,°
R1432 T1692 T1690 pobj °,at
R1433 T1693 T1689 npadvmod C,cultivated
R1434 T1694 T1689 prep with,cultivated
R1435 T1695 T1696 nummod 5,%
R1436 T1696 T1697 compound %,CO2
R1437 T1697 T1694 pobj CO2,with
R1438 T1698 T1689 punct .,cultivated
R1439 T1699 T1704 cc And,was
R1440 T1700 T1703 det the,medium
R1441 T1701 T1703 amod complete,medium
R1442 T1702 T1703 compound culture,medium
R1443 T1703 T1704 nsubj medium,was
R1444 T1704 T1704 ROOT was,was
R1445 T1705 T1704 acomp half-changed,was
R1446 T1706 T1704 advmod twice,was
R1447 T1707 T1708 det a,week
R1448 T1708 T1706 npadvmod week,twice
R1449 T1709 T1704 punct .,was
R1450 T1710 T1711 nsubj Astrocytes,expanded
R1451 T1711 T1711 ROOT expanded,expanded
R1452 T1712 T1711 prep for,expanded
R1453 T1713 T1714 advmod about,7
R1461 T1721 T1722 det the,flasks
R1462 T1722 T1726 nsubjpass flasks,shaken
R1463 T1723 T1722 cc or,flasks
R1464 T1724 T1722 conj dishes,flasks
R1465 T1725 T1726 auxpass were,shaken
R1466 T1726 T1726 ROOT shaken,shaken
R1467 T1727 T1726 prep at,shaken
R1468 T1728 T1729 nummod 150,rpm
R1469 T1729 T1727 pobj rpm,at
R1470 T1730 T1726 prep for,shaken
R1471 T1731 T1732 nummod 4,h
R1472 T1732 T1730 pobj h,for
R1473 T1733 T1734 aux to,deplete
R1474 T1734 T1726 advcl deplete,shaken
R1475 T1735 T1736 det the,microglia
R1476 T1736 T1734 dobj microglia,deplete
R1477 T1737 T1736 cc and,microglia
R1478 T1738 T1739 advmod less,adherent
R1479 T1739 T1740 amod adherent,cells
R1480 T1740 T1736 conj cells,microglia
R1481 T1741 T1734 prep from,deplete
R1482 T1742 T1743 det the,cultures
R1483 T1743 T1741 pobj cultures,from
R1484 T1744 T1726 punct .,shaken
R1485 T1745 T1751 prep After,were
R1486 T1746 T1745 pcomp shaking,After
R1487 T1747 T1751 punct ",",were
R1488 T1748 T1750 det the,cultures
R1489 T1749 T1750 amod resulting,cultures
R1490 T1750 T1751 nsubj cultures,were
R1491 T1751 T1751 ROOT were,were
R1492 T1752 T1753 advmod mainly,astrocytes
R1493 T1753 T1751 attr astrocytes,were
R1494 T1754 T1753 punct ",",astrocytes
R1495 T1755 T1757 nsubjpass which,determined
R1496 T1756 T1757 auxpass were,determined
R1497 T1757 T1753 relcl determined,astrocytes
R1498 T1758 T1757 agent by,determined
R1499 T1759 T1758 pobj immunoreactivity,by
R1500 T1760 T1757 prep for,determined
R1501 T1761 T1760 pobj GFAP,for
R1502 T1762 T1767 punct (,Biotechnology
R1503 T1763 T1767 amod sc-166481,Biotechnology
R1504 T1764 T1767 punct ",",Biotechnology
R1505 T1765 T1766 compound Santa,Cruz
R1506 T1766 T1767 compound Cruz,Biotechnology
R1507 T1767 T1761 appos Biotechnology,GFAP
R1508 T1768 T1767 punct ",",Biotechnology
R1509 T1769 T1767 conj CA,Biotechnology
R1510 T1770 T1769 punct ",",CA
R1511 T1771 T1767 meta USA,Biotechnology
R1512 T1772 T1767 punct ),Biotechnology
R1513 T1773 T1751 punct .,were
R1514 T1774 T1775 nsubj Cells,passaged
R1515 T1775 T1775 ROOT passaged,passaged
R1516 T1776 T1775 prep for,passaged
R1517 T1777 T1778 nummod 2-3,generations
R1518 T1778 T1776 pobj generations,for
R1519 T1779 T1780 auxpass were,used
R1520 T1780 T1775 conj used,passaged
R1521 T1781 T1780 prep in,used
R1522 T1782 T1784 det the,studies
R1523 T1783 T1784 amod following,studies
R1627 T1925 T1923 pobj Model,In
R1628 T1926 T1925 prep of,Model
R1629 T1927 T1926 pobj TBI,of
R1630 T1928 T1936 nsubjpass Established,performed
R1631 T1929 T1928 agent by,Established
R1632 T1930 T1934 compound Scratch,injury
R1633 T1931 T1932 compound Injury,Astrocyte
R1634 T1932 T1934 compound Astrocyte,injury
R1635 T1933 T1934 compound scratch,injury
R1636 T1934 T1929 pobj injury,by
R1637 T1935 T1936 auxpass was,performed
R1638 T1936 T1936 ROOT performed,performed
R1639 T1937 T1936 prep as,performed
R1640 T1938 T1937 prep in,as
R1641 T1939 T1941 det a,report
R1642 T1940 T1941 amod previous,report
R1643 T1941 T1938 pobj report,in
R1644 T1942 T1937 pobj [,as
R1645 T1943 T1942 nummod 7,[
R1646 T1944 T1942 conj ],[
R1647 T1945 T1936 punct .,performed
R1648 T1946 T1948 nsubjpass Astrocytes,planted
R1649 T1947 T1948 auxpass were,planted
R1650 T1948 T1948 ROOT planted,planted
R1651 T1949 T1948 prep in,planted
R1652 T1950 T1951 amod 6-well,plates
R1653 T1951 T1949 pobj plates,in
R1654 T1952 T1948 cc and,planted
R1655 T1953 T1948 conj grown,planted
R1656 T1954 T1953 prep to,grown
R1657 T1955 T1954 pobj confluence,to
R1658 T1956 T1948 punct .,planted
R1659 T1957 T1959 det The,monolayer
R1660 T1958 T1959 compound cell,monolayer
R1661 T1959 T1961 nsubjpass monolayer,scratched
R1662 T1960 T1961 auxpass was,scratched
R1663 T1961 T1961 ROOT scratched,scratched
R1664 T1962 T1961 prep with,scratched
R1665 T1963 T1967 det a,needle
R1666 T1964 T1967 amod sterile,needle
R1667 T1965 T1967 compound 26G,needle
R1668 T1966 T1967 compound syringe,needle
R1669 T1967 T1962 pobj needle,with
R1670 T1968 T1961 punct ",",scratched
R1671 T1969 T1961 advcl resulting,scratched
R1672 T1970 T1969 prep in,resulting
R1673 T1971 T1972 det the,formation
R1674 T1972 T1970 pobj formation,in
R1675 T1973 T1972 prep of,formation
R1676 T1974 T1978 det a,gap
R1677 T1975 T1976 nummod 0.5,mm
R1678 T1976 T1978 nmod mm,gap
R1679 T1977 T1978 amod wide,gap
R1680 T1978 T1973 pobj gap,of
R1681 T1979 T1961 punct .,scratched
R1682 T1980 T1981 advmod Immediately,after
R1683 T1981 T1986 prep after,washed
R1684 T1982 T1981 pobj scratch,after
R1685 T1983 T1986 punct ",",washed
R1686 T1984 T1986 nsubjpass cells,washed
R1687 T1985 T1986 auxpass were,washed
R1688 T1986 T1986 ROOT washed,washed
R1689 T1987 T1986 advmod twice,washed
R1690 T1988 T1986 prep with,washed
R1691 T1989 T1990 amod sterile,PBS
R1692 T1990 T1988 pobj PBS,with
R1693 T1991 T1986 punct ",",washed
R1694 T1992 T1986 conj cultured,washed
R1695 T1993 T1992 prep with,cultured
R1696 T1994 T1996 amod complete,medium
R1697 T1995 T1996 compound culture,medium
R1698 T1996 T1993 pobj medium,with
R1699 T1997 T1992 punct ",",cultured
R1700 T1998 T1992 cc and,cultured
R1701 T1999 T1992 conj named,cultured
R1702 T2000 T1999 prep as,named
R1703 T2001 T2002 compound TBI,group
R1704 T2002 T2000 pobj group,as
R1705 T2003 T1986 punct .,washed
R1706 T2004 T2013 nsubjpass Cells,used
R1707 T2005 T2004 punct ",",Cells
R1708 T2006 T2009 nsubjpass which,received
R1709 T2007 T2009 aux did,received
R1710 T2008 T2009 neg not,received
R1711 T2009 T2004 relcl received,Cells
R1712 T2010 T2009 dobj scratch,received
R1713 T2011 T2013 punct ",",used
R1714 T2012 T2013 auxpass were,used
R1715 T2013 T2013 ROOT used,used
R1716 T2014 T2013 prep as,used
R1717 T2015 T2016 amod blank,control
R1718 T2016 T2014 pobj control,as
R1719 T2017 T2013 cc and,used
R1720 T2018 T2013 conj named,used
R1721 T2019 T2018 prep as,named
R1722 T2020 T2021 amod sham,group
R1723 T2021 T2019 pobj group,as
R1724 T2022 T2013 punct .,used
R1844 T2171 T2171 ROOT 2.3,2.3
R1845 T2172 T2171 punct .,2.3
R1846 T2173 T2175 compound Cell,Measurement
R1847 T2174 T2175 compound Death,Measurement
R1848 T2175 T2184 nsubjpass Measurement,seeded
R1849 T2176 T2175 prep by,Measurement
R1850 T2177 T2178 compound CCK-8,Assay
R1851 T2178 T2176 pobj Assay,by
R1852 T2179 T2180 nummod 1,×
R1853 T2180 T2182 nummod ×,cells
R1854 T2181 T2182 amod 103/well,cells
R1855 T2182 T2184 nsubjpass cells,seeded
R1856 T2183 T2184 auxpass were,seeded
R1857 T2184 T2184 ROOT seeded,seeded
R1858 T2185 T2195 aux to,adhere
R1859 T2186 T2195 advmod 96-well,adhere
R1860 T2187 T2188 compound culture,plates
R1861 T2188 T2186 dobj plates,96-well
R1862 T2189 T2186 cc and,96-well
R1863 T2190 T2186 conj cultivated,96-well
R1864 T2191 T2195 mark for,adhere
R1865 T2192 T2193 nummod 24,h
R1866 T2193 T2195 nsubj h,adhere
R1867 T2194 T2195 aux to,adhere
R1868 T2195 T2184 xcomp adhere,seeded
R1869 T2196 T2184 punct .,seeded
R1870 T2197 T2201 advmod Then,scrated
R1871 T2198 T2199 det the,cell
R1872 T2199 T2201 nsubjpass cell,scrated
R1873 T2200 T2201 auxpass was,scrated
R1874 T2201 T2201 ROOT scrated,scrated
R1875 T2202 T2203 mark as,mentioned
R1876 T2203 T2201 advcl mentioned,scrated
R1877 T2204 T2203 advmod above,mentioned
R1878 T2205 T2201 punct .,scrated
R1879 T2206 T2208 compound Astrocyte,death
R1880 T2207 T2208 compound cell,death
R1881 T2208 T2210 nsubjpass death,assessed
R1882 T2209 T2210 auxpass was,assessed
R1883 T2210 T2210 ROOT assessed,assessed
R1884 T2211 T2210 agent by,assessed
R1885 T2212 T2211 pobj cell,by
R1886 T2213 T2210 advcl counting,assessed
R1887 T2214 T2216 nmod kit-8,CCK-8
R1888 T2215 T2216 punct (,CCK-8
R1889 T2216 T2223 nmod CCK-8,assay
R1890 T2217 T2216 punct ),CCK-8
R1891 T2218 T2223 punct (,assay
R1892 T2219 T2223 nmod Dojindo,assay
R1893 T2220 T2219 punct ",",Dojindo
R1894 T2221 T2223 nmod Japan,assay
R1895 T2222 T2223 punct ),assay
R1896 T2223 T2228 nsubj assay,according
R1897 T2224 T2225 nummod 24,h
R1898 T2225 T2226 npadvmod h,after
R1899 T2226 T2223 prep after,assay
R1900 T2227 T2226 pobj scratch,after
R1901 T2228 T2210 prep according,assessed
R1902 T2229 T2228 prep to,according
R1903 T2230 T2231 det the,manufacturer
R1904 T2231 T2233 poss manufacturer,protocol
R1905 T2232 T2231 case 's,manufacturer
R1906 T2233 T2229 pobj protocol,to
R1907 T2234 T2210 punct .,assessed
R1908 T2235 T2241 advmod Briefly,added
R1909 T2236 T2241 punct ",",added
R1910 T2237 T2239 nummod 10,CCK-8
R1911 T2238 T2239 compound μL,CCK-8
R1912 T2239 T2241 nsubjpass CCK-8,added
R1913 T2240 T2241 auxpass was,added
R1914 T2241 T2241 ROOT added,added
R1916 T2243 T2244 det every,well
R1917 T2244 T2242 pobj well,into
R1918 T2245 T2241 cc and,added
R1919 T2246 T2241 conj incubated,added
R1920 T2247 T2246 prep for,incubated
R1921 T2248 T2249 nummod 1,h
R1922 T2249 T2247 pobj h,for
R1923 T2250 T2241 punct .,added
R1924 T2251 T2255 advmod Then,read
R1925 T2252 T2253 compound OD,value
R1926 T2253 T2255 nsubjpass value,read
R1927 T2254 T2255 auxpass was,read
R1928 T2255 T2255 ROOT read,read
R1929 T2256 T2255 prep at,read
R1930 T2257 T2258 nummod 450,nm
R1931 T2258 T2256 pobj nm,at
R1932 T2259 T2255 advcl using,read
R1933 T2260 T2264 det a,reader
R1934 T2261 T2264 compound Bio-Rad,reader
R1935 T2262 T2264 compound ELISA,reader
R1936 T2263 T2264 compound microplate,reader
R1937 T2264 T2259 dobj reader,using
R1938 T2265 T2267 punct (,Laboratories
R1939 T2266 T2267 compound Bio-Rad,Laboratories
R1940 T2267 T2264 appos Laboratories,reader
R1941 T2268 T2267 punct ",",Laboratories
R1942 T2269 T2267 conj CA,Laboratories
R1943 T2270 T2269 punct ",",CA
R1944 T2271 T2269 conj USA,CA
R1945 T2272 T2267 punct ),Laboratories
R1946 T2273 T2255 punct .,read
R1947 T2274 T2275 det All,measurements
R1948 T2275 T2277 nsubjpass measurements,performed
R1949 T2276 T2277 auxpass were,performed
R1950 T2277 T2277 ROOT performed,performed
R1951 T2278 T2277 prep in,performed
R1952 T2279 T2278 pobj sextuplicate,in
R1953 T2280 T2277 punct .,performed
R1954 T2281 T2283 nsubjpass Results,expressed
R1955 T2282 T2283 auxpass were,expressed
R1956 T2283 T2283 ROOT expressed,expressed
R1957 T2284 T2283 prep as,expressed
R1958 T2285 T2284 pobj mean,as
R1959 T2286 T2285 prep of,mean
R1960 T2287 T2288 compound OD,value
R1961 T2288 T2286 pobj value,of
R1962 T2289 T2283 prep at,expressed
R1963 T2290 T2292 nummod 450,±
R1964 T2291 T2292 compound nm,±
R1965 T2292 T2293 compound ±,SD
R1966 T2293 T2289 pobj SD,at
R1967 T2294 T2283 punct .,expressed
R2313 T2674 T2674 ROOT 2.4,2.4
R2314 T2675 T2674 punct .,2.4
R2315 T2676 T2679 compound Electrophoretic,Assay
R2316 T2677 T2678 compound Mobility,Shift
R2317 T2678 T2679 compound Shift,Assay
R2318 T2679 T2687 nsubjpass Assay,washed
R2319 T2680 T2681 punct (,EMSA
R2320 T2681 T2679 appos EMSA,Assay
R2321 T2682 T2681 punct ),EMSA
R2322 T2683 T2679 appos Monolayers,Assay
R2323 T2684 T2683 prep of,Monolayers
R2324 T2685 T2684 pobj astrocytes,of
R2325 T2686 T2687 auxpass were,washed
R2326 T2687 T2687 ROOT washed,washed
R2327 T2688 T2687 prep with,washed
R2328 T2689 T2688 pobj PBS,with
R2329 T2690 T2687 cc and,washed
R2330 T2691 T2687 conj harvested,washed
R2331 T2692 T2691 prep by,harvested
R2332 T2693 T2692 pcomp scraping,by
R2333 T2694 T2693 prep into,scraping
R2334 T2695 T2696 amod cold,PBS
R2335 T2696 T2694 pobj PBS,into
R2336 T2697 T2687 punct .,washed
R2337 T2698 T2700 det The,pellet
R2338 T2699 T2700 compound cell,pellet
R2339 T2700 T2704 nsubj pellet,was
R2340 T2701 T2700 acl obtained,pellet
R2341 T2702 T2701 agent by,obtained
R2342 T2703 T2702 pobj centrifugation,by
R2343 T2704 T2705 auxpass was,resuspended
R2344 T2705 T2705 ROOT resuspended,resuspended
R2345 T2706 T2705 prep in,resuspended
R2346 T2707 T2706 pobj buffer,in
R2347 T2708 T2707 acl containing,buffer
R2348 T2709 T2711 nummod 10,HEPES
R2349 T2710 T2711 compound mM,HEPES
R2350 T2711 T2708 dobj HEPES,containing
R2351 T2712 T2713 punct (,pH
R2352 T2713 T2711 appos pH,HEPES
R2353 T2714 T2713 nummod 7.9,pH
R2359 T2720 T2719 punct ",",KCl
R2360 T2721 T2723 compound 0.1,EDTA
R2361 T2722 T2723 compound mM,EDTA
R2362 T2723 T2719 conj EDTA,KCl
R2363 T2724 T2723 punct ",",EDTA
R2364 T2725 T2727 nummod 0.1,EGTA
R2365 T2726 T2727 compound mM,EGTA
R2366 T2727 T2723 conj EGTA,EDTA
R2367 T2728 T2727 punct ",",EGTA
R2368 T2729 T2731 nummod 1.0,DTT
R2369 T2730 T2731 compound mM,DTT
R2370 T2731 T2727 conj DTT,EGTA
R2371 T2732 T2731 punct ",",DTT
R2372 T2733 T2731 cc and,DTT
R2373 T2734 T2737 nummod 0.5,fluoride
R2374 T2735 T2737 nmod mM,fluoride
R2375 T2736 T2737 compound phenylmethylsulfonyl,fluoride
R2376 T2737 T2731 conj fluoride,DTT
R2377 T2738 T2705 punct .,resuspended
R2378 T2739 T2745 advmod Then,added
R2379 T2740 T2741 nummod 10,%
R2380 T2741 T2743 compound %,P-40
R2381 T2742 T2743 compound Nonidet,P-40
R2382 T2743 T2745 nsubjpass P-40,added
R2383 T2744 T2745 auxpass was,added
R2384 T2745 T2745 ROOT added,added
R2385 T2746 T2745 cc and,added
R2386 T2747 T2745 conj vortexed,added
R2387 T2748 T2747 advmod briefly,vortexed
R2388 T2749 T2747 punct ",",vortexed
R2389 T2750 T2747 cc and,vortexed
R2390 T2751 T2752 det the,nuclei
R2391 T2752 T2754 nsubjpass nuclei,pelleted
R2392 T2753 T2754 auxpass were,pelleted
R2393 T2754 T2747 conj pelleted,vortexed
R2394 T2755 T2754 agent by,pelleted
R2395 T2756 T2755 pobj centrifugation,by
R2396 T2757 T2754 punct .,pelleted
R2397 T2758 T2760 det The,proteins
R2398 T2759 T2760 amod nuclear,proteins
R2399 T2760 T2762 nsubjpass proteins,extracted
R2400 T2761 T2762 auxpass were,extracted
R2401 T2762 T2762 ROOT extracted,extracted
R2402 T2763 T2762 prep with,extracted
R2403 T2764 T2763 pobj buffer,with
R2404 T2765 T2764 acl containing,buffer
R2405 T2766 T2768 nummod 20,HEPES
R2406 T2767 T2768 compound mM,HEPES
R2407 T2768 T2765 dobj HEPES,containing
R2408 T2769 T2770 punct (,pH
R2409 T2770 T2768 appos pH,HEPES
R2410 T2771 T2770 nummod 7.9,pH
R2411 T2772 T2770 punct ),pH
R2412 T2773 T2768 punct ",",HEPES
R2413 T2774 T2776 nummod 0.4,NaCl
R2414 T2775 T2776 compound mM,NaCl
R2415 T2776 T2768 appos NaCl,HEPES
R2416 T2777 T2776 punct ",",NaCl
R2417 T2778 T2780 nummod 1.0,EDTA
R2418 T2779 T2780 compound mM,EDTA
R2419 T2780 T2776 conj EDTA,NaCl
R2420 T2781 T2780 punct ",",EDTA
R2421 T2782 T2784 nummod 1.0,EGTA
R2422 T2783 T2784 compound mM,EGTA
R2423 T2784 T2780 conj EGTA,EDTA
R2424 T2785 T2784 punct ",",EGTA
R2425 T2786 T2788 nummod 1.0,DTT
R2452 T2813 T2814 auxpass was,stored
R2453 T2814 T2799 conj stored,removed
R2454 T2815 T2814 prep at,stored
R2455 T2816 T2818 nummod 80,C
R2456 T2817 T2818 nummod °,C
R2457 T2818 T2815 pobj C,at
R2458 T2819 T2814 prep until,stored
R2459 T2820 T2819 pobj use,until
R2460 T2821 T2814 punct .,stored
R2461 T2822 T2823 compound Protein,concentration
R2462 T2823 T2825 nsubjpass concentration,determined
R2463 T2824 T2825 auxpass was,determined
R2464 T2825 T2825 ROOT determined,determined
R2465 T2826 T2825 advcl using,determined
R2466 T2827 T2831 det a,kit
R2467 T2828 T2831 amod bicinchoninic,kit
R2468 T2829 T2830 compound acid,assay
R2469 T2830 T2831 compound assay,kit
R2470 T2831 T2826 dobj kit,using
R2471 T2832 T2831 prep with,kit
R2472 T2833 T2834 amod bovine,serum
R2473 T2834 T2832 pobj serum,with
R2474 T2835 T2831 amod albumin,kit
R2475 T2836 T2826 prep as,using
R2476 T2837 T2838 det the,standard
R2477 T2838 T2836 pobj standard,as
R2478 T2839 T2841 punct (,Biochemicals
R2479 T2840 T2841 compound Pierce,Biochemicals
R2480 T2841 T2826 parataxis Biochemicals,using
R2481 T2842 T2841 punct ",",Biochemicals
R2482 T2843 T2841 npadvmod Rockford,Biochemicals
R2483 T2844 T2843 punct ",",Rockford
R2484 T2845 T2843 conj IL,Rockford
R2485 T2846 T2845 punct ",",IL
R2486 T2847 T2843 npadvmod USA,Rockford
R2487 T2848 T2843 punct ),Rockford
R2488 T2849 T2841 punct .,Biochemicals
R2489 T2850 T2852 nsubjpass EMSA,performed
R2490 T2851 T2852 auxpass was,performed
R2491 T2852 T2852 ROOT performed,performed
R2492 T2853 T2852 advcl using,performed
R2493 T2854 T2855 compound gel,shift
R2494 T2855 T2857 compound shift,system
R2495 T2856 T2857 compound assay,system
R2496 T2857 T2853 dobj system,using
R2497 T2858 T2857 punct (,system
R2498 T2859 T2857 appos Promega,system
R2499 T2860 T2859 punct ",",Promega
R2500 T2861 T2859 conj Madison,Promega
R2501 T2862 T2861 punct ",",Madison
R2502 T2863 T2861 conj WI,Madison
R2503 T2864 T2863 punct ",",WI
R2518 T2879 T2879 ROOT end-labeled,end-labeled
R2519 T2880 T2879 agent by,end-labeled
R2520 T2881 T2882 compound T4-polynucleotide,kinase
R2521 T2882 T2880 pobj kinase,by
R2522 T2883 T2879 advcl using,end-labeled
R2523 T2884 T2888 compound [,ATP
R2524 T2885 T2886 compound γ-32P,]
R2525 T2886 T2888 compound ],ATP
R2526 T2887 T2888 punct -,ATP
R2527 T2888 T2883 dobj ATP,using
R2528 T2889 T2891 punct (,Biotech.
R2529 T2890 T2891 compound Free,Biotech.
R2530 T2891 T2888 appos Biotech.,ATP
R2531 T2892 T2891 punct ",",Biotech.
R2532 T2893 T2891 npadvmod Beijing,Biotech.
R2533 T2894 T2893 punct ",",Beijing
R2534 T2895 T2891 npadvmod China,Biotech.
R2535 T2896 T2891 punct ),Biotech.
R2536 T2897 T2879 punct .,end-labeled
R2537 T2898 T2899 compound Nuclear,protein
R2538 T2899 T2904 nsubj protein,was
R2539 T2900 T2899 punct (,protein
R2540 T2901 T2902 nummod 20,μg
R2541 T2902 T2899 appos μg,protein
R2542 T2903 T2899 punct ),protein
R2543 T2904 T2905 auxpass was,preincubated
R2544 T2905 T2905 ROOT preincubated,preincubated
R2545 T2906 T2905 prep in,preincubated
R2546 T2907 T2908 nummod 20,μL
R2547 T2908 T2910 nmod μL,buffer
R2548 T2909 T2910 amod binding,buffer
R2549 T2910 T2906 pobj buffer,in
R2550 T2911 T2910 acl containing,buffer
R2551 T2912 T2914 nummod 10,Tris-HCl
R2552 T2913 T2914 compound mM,Tris-HCl
R2553 T2914 T2911 dobj Tris-HCl,containing
R2554 T2915 T2916 punct (,PH
R2555 T2916 T2914 appos PH,Tris-HCl
R2556 T2917 T2916 nummod 7.5,PH
R2557 T2918 T2916 punct ),PH
R2558 T2919 T2914 punct ",",Tris-HCl
R2559 T2920 T2922 nummod 1,MgCl2
R2560 T2921 T2922 compound mM,MgCl2
R2561 T2922 T2914 conj MgCl2,Tris-HCl
R2562 T2923 T2922 punct ",",MgCl2
R2563 T2924 T2922 npadvmod 0.5,MgCl2
R2564 T2925 T2926 compound mM,NaCl
R2565 T2926 T2926 ROOT NaCl,NaCl
R2566 T2927 T2926 punct ",",NaCl
R2567 T2928 T2929 nummod 4,%
R2568 T2929 T2930 compound %,glycerol
R2569 T2930 T2926 appos glycerol,NaCl
R2570 T2931 T2930 punct ",",glycerol
R2571 T2932 T2934 nummod 0.5,EDTA
R2572 T2933 T2934 compound mM,EDTA
R2573 T2934 T2926 conj EDTA,NaCl
R2574 T2935 T2934 punct ",",EDTA
R2575 T2936 T2938 nummod 0.5,DTT
R2576 T2937 T2938 compound mM,DTT
R2577 T2938 T2934 conj DTT,EDTA
R2578 T2939 T2938 punct ",",DTT
R2579 T2940 T2938 cc and,DTT
R2580 T2941 T2942 nummod 2,μg
R2581 T2942 T2944 compound μg,dI-dC
R2582 T2943 T2944 compound poly,dI-dC
R2583 T2944 T2934 appos dI-dC,EDTA
R2584 T2945 T2944 prep for,dI-dC
R2585 T2946 T2947 nummod 20,minutes
R2586 T2947 T2945 pobj minutes,for
R2587 T2948 T2947 prep on,minutes
R2588 T2949 T2948 pobj ice,on
R2589 T2950 T2926 punct .,NaCl
R2590 T2951 T2964 prep After,continued
R2591 T2952 T2951 pobj addition,After
R2592 T2953 T2952 prep of,addition
R2593 T2954 T2959 det the,probe
R2594 T2955 T2956 nummod 1,μL
R2611 T2972 T2973 amod DNA-protein,complexes
R2612 T2973 T2975 nsubjpass complexes,separated
R2613 T2974 T2975 auxpass were,separated
R2614 T2975 T2975 ROOT separated,separated
R2615 T2976 T2975 agent by,separated
R2616 T2977 T2976 pobj electrophoresis,by
R2617 T2978 T2975 prep on,separated
R2618 T2979 T2980 nummod 4,%
R2619 T2980 T2983 nmod %,gel
R2620 T2981 T2983 amod nondenaturing,gel
R2621 T2982 T2983 compound polyacrylamide,gel
R2622 T2983 T2978 pobj gel,on
R2623 T2984 T2975 prep in,separated
R2624 T2985 T2988 nummod 0.5,buffer
R2625 T2986 T2988 nummod ×,buffer
R2626 T2987 T2988 compound TBE,buffer
R2627 T2988 T2984 pobj buffer,in
R2628 T2989 T2988 punct (,buffer
R2629 T2990 T2988 appos tris-borate-EDTA,buffer
R2630 T2991 T2988 punct ),buffer
R2631 T2992 T2975 prep at,separated
R2632 T2993 T2994 nummod 390,V
R2633 T2994 T2992 pobj V,at
R2634 T2995 T2975 prep for,separated
R2635 T2996 T2997 nummod 1,hour
R2636 T2997 T2995 pobj hour,for
R2637 T2998 T2975 prep at,separated
R2638 T2999 T3000 nummod 4,°
R2664 T3025 T3024 prep of,Levels
R2665 T3026 T3027 compound NF-κB,DNA
R2666 T3027 T3025 pobj DNA,of
R2667 T3028 T3029 amod binding,activity
R2668 T3029 T3025 pobj activity,of
R2669 T3030 T3031 auxpass were,quantified
R2670 T3031 T3031 ROOT quantified,quantified
R2671 T3032 T3031 agent by,quantified
R2672 T3033 T3034 compound software,ImageJ
R2673 T3034 T3032 pobj ImageJ,by
R2674 T3035 T3031 punct .,quantified
R2782 T3233 T3233 ROOT 2.5,2.5
R2783 T3234 T3233 punct .,2.5
R2784 T3235 T3237 compound RT-PCR,RNA
R2785 T3236 T3237 compound Total,RNA
R2786 T3237 T3238 nsubj RNA,was
R2787 T3238 T3238 ROOT was,was
R2788 T3239 T3238 acomp isolated,was
R2789 T3240 T3239 prep with,isolated
R2790 T3241 T3240 pobj Trizol,with
R2791 T3242 T3254 punct (,synthesized
R2792 T3243 T3252 nmod Invitrogen,cDNA
R2793 T3244 T3243 punct ",",Invitrogen
R2794 T3245 T3243 conj CA,Invitrogen
R2795 T3246 T3245 punct ",",CA
R2796 T3247 T3245 conj USA,CA
R2797 T3248 T3245 punct ),CA
R2798 T3249 T3245 punct ",",CA
R2799 T3250 T3245 cc and,CA
R2800 T3251 T3252 compound single-stranded,cDNA
R2801 T3252 T3254 nsubjpass cDNA,synthesized
R2802 T3253 T3254 auxpass was,synthesized
R2803 T3254 T3238 parataxis synthesized,was
R2804 T3255 T3254 prep from,synthesized
R2805 T3256 T3257 nummod 2,μg
R2806 T3257 T3255 pobj μg,from
R2807 T3258 T3257 prep of,μg
R2808 T3259 T3260 amod total,RNA
R2809 T3260 T3258 pobj RNA,of
R2810 T3261 T3257 prep with,μg
R2811 T3262 T3263 amod BU-Script,RT-Kit
R2812 T3263 T3261 pobj RT-Kit,with
R2813 T3264 T3265 punct (,Biunique
R2814 T3265 T3263 appos Biunique,RT-Kit
R2815 T3266 T3265 punct ",",Biunique
R2816 T3267 T3265 conj Jiangsu,Biunique
R2817 T3268 T3267 punct ",",Jiangsu
R2818 T3269 T3267 conj China,Jiangsu
R2819 T3270 T3254 punct ),synthesized
R2820 T3271 T3238 prep according,was
R2821 T3272 T3271 prep to,according
R2822 T3273 T3274 det the,manufacturer
R2823 T3274 T3276 poss manufacturer,protocol
R2824 T3275 T3274 case 's,manufacturer
R2825 T3276 T3272 pobj protocol,to
R2826 T3277 T3238 punct .,was
R2827 T3278 T3279 det The,cDNA
R2828 T3279 T3281 nsubjpass cDNA,stored
R2829 T3280 T3281 auxpass was,stored
R2830 T3281 T3281 ROOT stored,stored
R2831 T3282 T3281 prep in,stored
R2832 T3283 T3286 nummod −,C.
R2833 T3284 T3285 nummod 20,°
R2834 T3285 T3286 compound °,C.
R2835 T3286 T3282 pobj C.,in
R2836 T3287 T3288 amod Reverse,transcription
R2837 T3288 T3290 nsubjpass transcription,conducted
R2838 T3289 T3290 auxpass was,conducted
R2839 T3290 T3281 conj conducted,stored
R2840 T3291 T3290 prep with,conducted
R2841 T3292 T3295 compound GoTaq,Mix
R2842 T3293 T3295 compound Green,Mix
R2843 T3294 T3295 compound Master,Mix
R2844 T3295 T3291 pobj Mix,with
R2845 T3296 T3295 punct (,Mix
R2846 T3297 T3295 appos Promega,Mix
R2847 T3298 T3297 punct ",",Promega
R2848 T3299 T3297 conj WI,Promega
R2849 T3300 T3299 punct ",",WI
R2850 T3301 T3299 appos USA,WI
R2851 T3302 T3295 punct ),Mix
R2852 T3303 T3290 prep according,conducted
R2853 T3304 T3303 prep to,according
R2854 T3305 T3306 det the,manufacturer
R2855 T3306 T3308 poss manufacturer,protocol
R2856 T3307 T3306 case 's,manufacturer
R2857 T3308 T3304 pobj protocol,to
R2858 T3309 T3290 punct .,conducted
R2859 T3310 T3312 nsubj Table,shows
R2860 T3311 T3310 nummod 1,Table
R2861 T3312 T3312 ROOT shows,shows
R2862 T3313 T3314 det the,primers
R2863 T3314 T3312 dobj primers,shows
R2864 T3315 T3314 cc and,primers
R2865 T3316 T3317 compound PCR,parameters
R2866 T3317 T3314 conj parameters,primers
R2867 T3318 T3312 punct .,shows
R2868 T3319 T3320 compound PCR,products
R2869 T3320 T3322 nsubjpass products,detected
R2870 T3321 T3322 auxpass were,detected
R2871 T3322 T3322 ROOT detected,detected
R2872 T3323 T3322 agent by,detected
R2873 T3324 T3326 amod agarose,electrophoresis
R2874 T3325 T3326 compound gel,electrophoresis
R2875 T3326 T3323 pobj electrophoresis,by
R2876 T3327 T3322 punct .,detected
R2877 T3328 T3329 det The,intensity
R2878 T3329 T3334 nsubjpass intensity,analyzed
R2879 T3330 T3329 prep of,intensity
R2880 T3331 T3332 det the,bands
R2881 T3332 T3330 pobj bands,of
R2882 T3333 T3334 auxpass was,analyzed
R2883 T3334 T3334 ROOT analyzed,analyzed
R2884 T3335 T3334 agent by,analyzed
R2885 T3336 T3337 compound ImageJ,program
R2886 T3337 T3335 pobj program,by
R2887 T3338 T3334 punct .,analyzed
R2888 T3339 T3340 det The,level
R2889 T3340 T3344 nsubjpass level,used
R2890 T3341 T3340 prep of,level
R2891 T3342 T3341 pobj β-actin,of
R2892 T3343 T3344 auxpass was,used
R2893 T3344 T3344 ROOT used,used
R2894 T3345 T3344 prep as,used
R2895 T3346 T3348 det an,standard
R2896 T3347 T3348 amod internal,standard
R2897 T3348 T3345 pobj standard,as
R2898 T3349 T3344 punct .,used
R3191 T3697 T3697 ROOT 2.6,2.6
R3192 T3698 T3697 punct .,2.6
R3193 T3699 T3700 compound Western,Blot
R3194 T3700 T3702 nsubj Blot,obtain
R3195 T3701 T3702 aux To,obtain
R3196 T3702 T3702 ROOT obtain,obtain
R3197 T3703 T3704 amod total,protein
R3198 T3704 T3702 dobj protein,obtain
R3199 T3705 T3709 advmod lysates,homogenized
R3200 T3706 T3709 punct ",",homogenized
R3201 T3707 T3709 nsubjpass cells,homogenized
R3202 T3708 T3709 auxpass were,homogenized
R3203 T3709 T3702 advcl homogenized,obtain
R3204 T3710 T3709 prep in,homogenized
R3205 T3711 T3712 compound RIPA,buffer
R3206 T3712 T3710 pobj buffer,in
R3207 T3713 T3715 punct (,%
R3208 T3714 T3715 nummod 1,%
R3209 T3715 T3775 nsubjpass %,estimated
R3210 T3716 T3721 nummod NP40,deoxycholate
R3211 T3717 T3716 punct ",",NP40
R3212 T3718 T3719 nummod 0.5,%
R3213 T3719 T3721 nmod %,deoxycholate
R3214 T3720 T3721 compound sodium,deoxycholate
R3215 T3721 T3715 appos deoxycholate,%
R3216 T3722 T3721 punct ",",deoxycholate
R3217 T3723 T3724 nummod 0.1,%
R3218 T3724 T3715 appos %,%
R3219 T3725 T3715 npadvmod SDS,%
R3220 T3726 T3725 punct ",",SDS
R3221 T3727 T3729 nummod 1,EDTA
R3222 T3728 T3729 compound mM,EDTA
R3223 T3729 T3725 appos EDTA,SDS
R3224 T3730 T3725 punct ",",SDS
R3225 T3731 T3733 nummod 1,EGTA
R3226 T3732 T3733 compound mM,EGTA
R3227 T3733 T3725 appos EGTA,SDS
R3228 T3734 T3725 punct ",",SDS
R3229 T3735 T3737 nummod 1,Na3VO4
R3230 T3736 T3737 compound mM,Na3VO4
R3231 T3737 T3725 appos Na3VO4,SDS
R3232 T3738 T3725 punct ",",SDS
R3233 T3739 T3741 nummod 20,NaF
R3234 T3740 T3741 compound mM,NaF
R3235 T3741 T3725 appos NaF,SDS
R3236 T3742 T3725 punct ",",SDS
R3237 T3743 T3745 nummod 0.5,DTT
R3238 T3744 T3745 compound mM,DTT
R3239 T3745 T3725 appos DTT,SDS
R3240 T3746 T3725 punct ",",SDS
R3241 T3747 T3749 nummod 1,PMSF
R3242 T3748 T3749 compound mM,PMSF
R3243 T3749 T3725 appos PMSF,SDS
R3244 T3750 T3725 punct ",",SDS
R3245 T3751 T3715 cc and,%
R3246 T3752 T3753 compound protease,inhibitor
R3247 T3753 T3754 compound inhibitor,cocktail
R3248 T3754 T3715 conj cocktail,%
R3297 T3803 T3804 compound loading,buffer
R3298 T3804 T3800 pobj buffer,in
R3299 T3805 T3799 punct ",",heat-denatured
R3300 T3806 T3798 advcl separated,were
R3301 T3807 T3806 agent by,separated
R3302 T3808 T3809 nummod 10,%
R3303 T3809 T3815 compound %,electrophoresis
R3304 T3810 T3812 compound sodium,sulfate
R3305 T3811 T3812 compound dodecyl,sulfate
R3306 T3812 T3814 compound sulfate,gel
R3307 T3813 T3814 compound polyacrylamide,gel
R3308 T3814 T3815 compound gel,electrophoresis
R3309 T3815 T3807 pobj electrophoresis,by
R3310 T3816 T3806 punct ",",separated
R3311 T3817 T3806 cc and,separated
R3312 T3818 T3806 conj electroblotted,separated
R3313 T3819 T3818 prep onto,electroblotted
R3314 T3820 T3822 det a,membrane
R3315 T3821 T3822 amod nitrocellulose,membrane
R3316 T3822 T3819 pobj membrane,onto
R3317 T3823 T3798 punct .,were
R3318 T3824 T3829 prep For,blocked
R3319 T3825 T3824 pobj immunoblotting,For
R3320 T3826 T3829 punct ",",blocked
R3321 T3827 T3829 nsubjpass membranes,blocked
R3322 T3828 T3829 auxpass were,blocked
R3323 T3829 T3829 ROOT blocked,blocked
R3324 T3830 T3829 prep with,blocked
R3325 T3831 T3832 nummod 5,%
R3326 T3832 T3835 nmod %,milk
R3327 T3833 T3835 amod nonfat,milk
R3328 T3834 T3835 amod dry,milk
R3329 T3835 T3830 pobj milk,with
R3330 T3836 T3835 prep in,milk
R3331 T3837 T3838 compound saline,buffer
R3332 T3838 T3839 compound buffer,overnight
R3333 T3839 T3836 pobj overnight,in
R3355 T3861 T3860 punct ",",CA
R3356 T3862 T3860 conj USA,CA
R3357 T3863 T3860 punct ",",CA
R3358 T3864 T3865 nummod 43,kDa
R3359 T3865 T3860 appos kDa,CA
R3360 T3866 T3865 punct ),kDa
R3361 T3867 T3865 cc and,kDa
R3362 T3868 T3869 amod anti-matrix,metallopeptidase
R3363 T3869 T3865 conj metallopeptidase,kDa
R3364 T3870 T3869 nummod 9,metallopeptidase
R3365 T3871 T3872 punct (,MMP9
R3366 T3872 T3869 appos MMP9,metallopeptidase
R3367 T3873 T3872 punct ),MMP9
R3368 T3874 T3875 punct (,sc-6841
R3369 T3875 T3869 appos sc-6841,metallopeptidase
R3370 T3876 T3875 punct ",",sc-6841
R3371 T3877 T3879 compound Santa,Biotechnology
R3372 T3878 T3879 compound Cruz,Biotechnology
R3373 T3879 T3875 appos Biotechnology,sc-6841
R3374 T3880 T3879 punct ",",Biotechnology
R3375 T3881 T3879 conj CA,Biotechnology
R3376 T3882 T3881 punct ",",CA
R3377 T3883 T3881 conj USA,CA
R3378 T3884 T3881 punct ",",CA
R3379 T3885 T3886 nummod 92,kDa
R3380 T3886 T3881 appos kDa,CA
R3381 T3887 T3875 punct ),sc-6841
R3382 T3888 T3854 punct .,sc-130657
R3383 T3889 T3891 det Each,antibody
R3384 T3890 T3891 amod primary,antibody
R3385 T3891 T3893 nsubjpass antibody,diluted
R3386 T3892 T3893 auxpass was,diluted
R3387 T3893 T3893 ROOT diluted,diluted
R3388 T3894 T3893 advmod appropriately,diluted
R3389 T3895 T3893 prep in,diluted
R3390 T3896 T3895 pcomp blocking,in
R3391 T3897 T3896 dobj buffer,blocking
R3392 T3898 T3893 cc and,diluted
R3393 T3899 T3900 advmod then,added
R3394 T3900 T3893 conj added,diluted
R3395 T3901 T3900 prep to,added
R3396 T3902 T3903 det the,blots
R3397 T3903 T3901 pobj blots,to
R3398 T3904 T3900 prep for,added
R3399 T3905 T3906 nummod 1,h
R3400 T3906 T3904 pobj h,for
R3401 T3907 T3906 prep at,h
R3402 T3908 T3909 compound room,temperature
R3403 T3909 T3907 pobj temperature,at
R3404 T3910 T3900 punct .,added
R3405 T3911 T3912 det The,blots
R3406 T3912 T3914 nsubjpass blots,washed
R3407 T3913 T3914 auxpass were,washed
R3408 T3914 T3914 ROOT washed,washed
R3409 T3915 T3916 nummod three,times
R3410 T3916 T3914 npadvmod times,washed
R3433 T3939 T3940 auxpass were,incubated
R3434 T3940 T3914 conj incubated,washed
R3435 T3941 T3940 prep with,incubated
R3436 T3942 T3943 amod enhanced,chemiluminescence
R3437 T3943 T3941 pobj chemiluminescence,with
R3438 T3944 T3945 punct (,ECL
R3439 T3945 T3943 appos ECL,chemiluminescence
R3440 T3946 T3945 punct ),ECL
R3441 T3947 T3948 compound detection,system
R3442 T3948 T3943 conj system,chemiluminescence
R3443 T3949 T3948 punct (,system
R3444 T3950 T3951 compound Amersham,Biosciences
R3445 T3951 T3948 appos Biosciences,system
R3446 T3952 T3951 punct ",",Biosciences
R3447 T3953 T3951 conj Bucks,Biosciences
R3448 T3954 T3953 punct ",",Bucks
R3449 T3955 T3953 conj UK,Bucks
R3450 T3956 T3953 punct ),Bucks
R3451 T3957 T3940 cc and,incubated
R3452 T3958 T3940 conj exposed,incubated
R3453 T3959 T3958 prep to,exposed
R3454 T3960 T3961 amod radiographic,film
R3455 T3961 T3959 pobj film,to
R3456 T3962 T3964 punct (,Hyperfilm
R3457 T3963 T3964 compound Fuji,Hyperfilm
R3458 T3964 T3961 appos Hyperfilm,film
R3459 T3965 T3964 punct ",",Hyperfilm
R3460 T3966 T3964 conj Tokyo,Hyperfilm
R3461 T3967 T3966 punct ",",Tokyo
R3462 T3968 T3966 conj Japan,Tokyo
R3463 T3969 T3964 punct ),Hyperfilm
R3464 T3970 T3940 punct .,incubated
R3465 T3971 T3973 nsubjpass ImageJ,used
R3466 T3972 T3973 auxpass was,used
R3467 T3973 T3973 ROOT used,used
R3468 T3974 T3975 aux to,analyze
R3469 T3975 T3973 xcomp analyze,used
R3470 T3976 T3977 det the,intensity
R3471 T3977 T3975 dobj intensity,analyze
R3472 T3978 T3977 prep of,intensity
R3473 T3979 T3980 det the,blots
R3474 T3980 T3978 pobj blots,of
R3475 T3981 T3973 punct .,used
R3476 T3982 T3983 det The,level
R3477 T3983 T3987 nsubjpass level,used
R3478 T3984 T3983 prep of,level
R3479 T3985 T3984 pobj β-actin,of
R3480 T3986 T3987 auxpass was,used
R3481 T3987 T3987 ROOT used,used
R3482 T3988 T3987 prep as,used
R3483 T3989 T3990 amod internal,standard
R3484 T3990 T3988 pobj standard,as
R3485 T3991 T3987 punct .,used
R3873 T4560 T4560 ROOT 2.8,2.8
R3874 T4561 T4560 punct .,2.8
R3875 T4562 T4564 compound Gelatine,Cells
R3887 T4574 T4576 nummod 50,Tris-HCl
R3888 T4575 T4576 compound mM,Tris-HCl
R3889 T4576 T4573 dobj Tris-HCl,containing
R3890 T4577 T4578 punct (,pH
R3891 T4578 T4576 appos pH,Tris-HCl
R3892 T4579 T4578 nummod 7.4,pH
R3893 T4580 T4578 punct ),pH
R3894 T4581 T4576 punct ",",Tris-HCl
R3895 T4582 T4584 nummod 150,NaCl
R3896 T4583 T4584 compound mM,NaCl
R3897 T4584 T4576 conj NaCl,Tris-HCl
R3898 T4585 T4584 punct ",",NaCl
R3899 T4586 T4588 nummod 5,CaCl2
R3900 T4587 T4588 compound mM,CaCl2
R3901 T4588 T4584 conj CaCl2,NaCl
R3902 T4589 T4588 punct ",",CaCl2
R3903 T4590 T4592 nummod 0.2,NaN3
R3904 T4591 T4592 compound mM,NaN3
R3905 T4592 T4588 conj NaN3,CaCl2
R3906 T4593 T4592 punct ",",NaN3
R3907 T4594 T4592 cc and,NaN3
R3908 T4595 T4596 nummod 0.01,%
R3909 T4596 T4597 compound %,Triton
R3910 T4597 T4592 conj Triton,NaN3
R3911 T4598 T4569 punct .,homogenized
R3912 T4599 T4600 amod Soluble,extracts
R3913 T4600 T4602 nsubjpass extracts,separated
R3914 T4601 T4602 auxpass were,separated
R3915 T4602 T4602 ROOT separated,separated
R3916 T4603 T4602 agent by,separated
R3917 T4604 T4603 pobj centrifugation,by
R3918 T4605 T4602 cc and,separated
R3919 T4606 T4614 nsubjpass stored,performed
R3920 T4607 T4606 prep at,stored
R3921 T4608 T4609 nummod 20,°
R3922 T4609 T4612 compound °,zymography
R3923 T4610 T4612 compound C.,zymography
R3924 T4611 T4612 compound Gelatin,zymography
R3925 T4612 T4607 pobj zymography,at
R3926 T4613 T4614 auxpass was,performed
R3927 T4614 T4602 conj performed,separated
R3928 T4615 T4614 xcomp according,performed
R3929 T4616 T4615 prep to,according
R3930 T4617 T4618 det the,manufacturer
R3931 T4618 T4620 poss manufacturer,instructions
R3932 T4619 T4618 case 's,manufacturer
R3933 T4620 T4616 pobj instructions,to
R3934 T4621 T4624 punct (,Inc
R3935 T4622 T4624 compound Genmed,Inc
R3936 T4623 T4624 compound Scientifics,Inc
R3937 T4624 T4620 appos Inc,instructions
R3938 T4625 T4624 punct ",",Inc
R3939 T4626 T4624 conj MA,Inc
R3940 T4627 T4626 punct ",",MA
R3941 T4628 T4626 appos USA,MA
R3942 T4629 T4626 punct ),MA
R3943 T4630 T4614 punct .,performed
R3944 T4631 T4639 advmod Briefly,separated
R3945 T4632 T4639 punct ",",separated
R3946 T4633 T4637 nummod 40,extracts
R3947 T4634 T4637 compound μg,extracts
R3948 T4635 T4636 compound cytosolic,protein
R3949 T4636 T4637 compound protein,extracts
R3950 T4637 T4639 nsubjpass extracts,separated
R3951 T4638 T4639 auxpass were,separated
R3952 T4639 T4639 ROOT separated,separated
R3953 T4640 T4639 prep by,separated
R3954 T4641 T4640 pobj electrophoresis,by
R3955 T4642 T4639 punct .,separated
R3956 T4643 T4647 advmod Then,renatured
R3957 T4644 T4645 det the,proteins
R3958 T4645 T4647 nsubjpass proteins,renatured
R3959 T4646 T4647 auxpass were,renatured
R3960 T4647 T4647 ROOT renatured,renatured
R3961 T4648 T4647 agent by,renatured
R3962 T4649 T4648 pobj incubation,by
R3963 T4650 T4647 prep in,renatured
R3975 T4662 T4663 nummod 40,h
R3976 T4663 T4661 pobj h,for
R3977 T4664 T4657 prep at,incubated
R3978 T4665 T4666 nummod 37,°
R3979 T4666 T4664 pobj °,at
R3980 T4667 T4657 conj C,incubated
R3981 T4668 T4669 aux to,enable
R3982 T4669 T4657 advcl enable,incubated
R3983 T4670 T4671 det the,MMP9
R3984 T4671 T4669 dobj MMP9,enable
R3985 T4672 T4673 aux to,cleave
R3986 T4673 T4669 xcomp cleave,enable
R3987 T4674 T4675 det the,gelatin
R3988 T4675 T4673 dobj gelatin,cleave
R3989 T4676 T4647 punct .,renatured
R3990 T4677 T4685 prep After,stained
R3991 T4678 T4677 pcomp rinsing,After
R3992 T4679 T4678 prep in,rinsing
R3993 T4680 T4679 pobj water,in
R3994 T4681 T4685 punct ",",stained
R3995 T4682 T4683 det each,gel
R3996 T4683 T4685 nsubjpass gel,stained
R3997 T4684 T4685 auxpass was,stained
R3998 T4685 T4685 ROOT stained,stained
R3999 T4686 T4685 prep with,stained
R4000 T4687 T4688 compound Coomassie,blue
R4001 T4688 T4686 pobj blue,with
R4002 T4689 T4688 prep for,blue
R4003 T4690 T4691 nummod 1,h
R4004 T4691 T4689 pobj h,for
R4005 T4692 T4691 cc and,h
R4006 T4693 T4688 conj destained,blue
R4007 T4694 T4693 prep in,destained
R4008 T4695 T4696 nummod 50,%
R4009 T4696 T4697 compound %,methanol
R4010 T4697 T4694 pobj methanol,in
R4011 T4698 T4685 punct .,stained
R4012 T4699 T4700 amod Proteolytic,activities
R4013 T4700 T4702 nsubjpass activities,showed
R4014 T4701 T4702 auxpass were,showed
R4015 T4702 T4702 ROOT showed,showed
R4016 T4703 T4702 agent by,showed
R4017 T4704 T4705 amod clear,bands
R4018 T4705 T4703 pobj bands,by
R4019 T4706 T4705 prep in,bands
R4020 T4707 T4708 amod blue,gel
R4021 T4708 T4706 pobj gel,in
R4022 T4709 T4710 nsubj which,indicates
R4023 T4710 T4702 advcl indicates,showed
R4024 T4711 T4712 det the,lysis
R4025 T4712 T4710 dobj lysis,indicates
R4026 T4713 T4712 prep of,lysis
R4027 T4714 T4715 det the,substrate
R4028 T4715 T4713 pobj substrate,of
R4029 T4716 T4702 punct .,showed
R4030 T4717 T4723 nsubjpass Quantification,performed
R4031 T4718 T4717 prep of,Quantification
R4082 T4799 T4799 ROOT 2.9,2.9
R4083 T4800 T4799 punct .,2.9
R4084 T4801 T4802 compound Statistical,Analysis
R4085 T4802 T4803 compound Analysis,Data
R4086 T4803 T4805 nsubjpass Data,expressed
R4087 T4804 T4805 auxpass were,expressed
R4088 T4805 T4805 ROOT expressed,expressed
R4089 T4806 T4811 mark as,evaluated
R4090 T4807 T4809 amod mean,SD
R4091 T4808 T4809 compound ±,SD
R4092 T4809 T4811 nsubj SD,evaluated
R4093 T4810 T4809 cc and,SD
R4094 T4811 T4805 advcl evaluated,expressed
R4095 T4812 T4811 agent by,evaluated
R4096 T4813 T4812 pobj ANOVA,by
R4097 T4814 T4813 cc and,ANOVA
R4098 T4815 T4813 conj LSD,ANOVA
R4099 T4816 T4818 amod multiple,test
R4100 T4817 T4818 compound comparison,test
R4101 T4818 T4811 dobj test,evaluated
R4102 T4819 T4805 punct .,expressed
R4103 T4820 T4821 compound P,values
R4104 T4821 T4822 nsubj values,<
R4105 T4822 T4825 dep <,considered
R4106 T4823 T4825 nsubjpass 0.05,considered
R4107 T4824 T4825 auxpass were,considered
R4108 T4825 T4825 ROOT considered,considered
R4109 T4826 T4827 aux to,be
R4110 T4827 T4825 xcomp be,considered
R4111 T4828 T4827 acomp significant,be
R4112 T4829 T4825 punct .,considered
R4113 T4830 T4831 det All,analyses
R4114 T4831 T4833 nsubjpass analyses,performed
R4115 T4832 T4833 auxpass were,performed
R4116 T4833 T4833 ROOT performed,performed
R4117 T4834 T4833 prep by,performed
R4118 T4835 T4834 pcomp using,by
R4119 T4836 T4838 nmod SPSS,software
R4120 T4837 T4836 nummod 18.0,SPSS
R4121 T4838 T4835 dobj software,using
R4122 T4839 T4833 punct .,performed
R4267 T5012 T5014 amod cytoplasmic,distortion
R4268 T5013 T5014 compound process,distortion
R4269 T5014 T5017 nsubjpass distortion,observed
R4270 T5015 T5017 auxpass were,observed
R4271 T5016 T5017 advmod mostly,observed
R4272 T5017 T5009 conj observed,lost
R4273 T5018 T5017 prep in,observed
R4274 T5019 T5020 det both,sides
R4275 T5020 T5018 pobj sides,in
R4276 T5021 T5020 prep of,sides
R4277 T5022 T5023 compound scratch,line
R4278 T5023 T5021 pobj line,of
R4279 T5024 T5023 prep in,line
R4280 T5025 T5027 compound group,TBI
R4281 T5026 T5027 compound KO,TBI
R4282 T5027 T5024 pobj TBI,in
R4283 T5028 T5017 punct .,observed
R4284 T5029 T5030 nummod CCK-8,assay
R4285 T5030 T5032 nsubj assay,showed
R4286 T5031 T5032 advmod also,showed
R4287 T5032 T5032 ROOT showed,showed
R4288 T5033 T5035 amod lower,value
R4289 T5034 T5035 compound OD,value
R4290 T5035 T5032 dobj value,showed
R4291 T5036 T5035 prep of,value
R4292 T5037 T5039 compound group,TBI
R4293 T5038 T5039 compound KO,TBI
R4294 T5039 T5036 pobj TBI,of
R4295 T5040 T5041 punct (,0.38
R4296 T5041 T5043 nummod 0.38,0.064
R4297 T5042 T5043 nummod ±,0.064
R4298 T5043 T5039 appos 0.064,TBI
R4299 T5044 T5032 punct ),showed
R4300 T5045 T5032 prep than,showed
R4301 T5046 T5045 pobj that,than
R4302 T5047 T5046 prep of,that
R4303 T5048 T5047 pobj group,of
R4304 T5049 T5050 compound WT,TBI
R4305 T5050 T5048 appos TBI,group
R4306 T5051 T5052 punct (,0.98
R4307 T5052 T5054 nummod 0.98,0.098
R4308 T5053 T5054 nummod ±,0.098
R4309 T5054 T5048 appos 0.098,group
R4310 T5055 T5032 punct ),showed
R4311 T5056 T5032 punct (,showed
R4312 T5057 T5057 ROOT P,P
R4313 T5058 T5057 nummod <,P
R4314 T5059 T5057 nummod 0.01,P
R4315 T5060 T5057 punct ),P
R4316 T5061 T5057 punct ",",P
R4317 T5062 T5066 nsubj which,suggested
R4319 T5065 T5066 advmod also,suggested
R4320 T5066 T5057 relcl suggested,P
R4321 T5067 T5069 amod more,death
R4322 T5068 T5069 compound cell,death
R4323 T5069 T5066 dobj death,suggested
R4324 T5070 T5069 prep in,death
R4325 T5071 T5073 compound group,TBI
R4326 T5072 T5073 compound KO,TBI
R4327 T5073 T5070 pobj TBI,in
R4328 T5074 T5075 punct (,Figure
R4329 T5075 T5073 appos Figure,TBI
R4330 T5076 T5075 nummod 1,Figure
R4331 T5077 T5073 punct ),TBI
R4332 T5078 T5057 punct .,P
R4333 T5079 T5080 expl There,was
R4334 T5080 T5080 ROOT was,was
R4335 T5081 T5082 det no,difference
R4336 T5082 T5080 attr difference,was
R4337 T5083 T5082 prep between,difference
R4338 T5084 T5086 nummod two,groups
R4339 T5085 T5086 amod sham-operated,groups
R4340 T5086 T5083 pobj groups,between
R4341 T5087 T5080 punct .,was
R4452 T5234 T5233 prep of,Upregulation
R4453 T5235 T5236 compound NF-κB,DNA-Binding
R4454 T5236 T5234 pobj DNA-Binding,of
R4455 T5237 T5229 conj Activity,Disruption
R4456 T5238 T5229 prep after,Disruption
R4457 T5239 T5240 compound Scratch,Injury
R4458 T5240 T5238 pobj Injury,after
R4459 T5241 T5244 nsubjpass It,reported
R4460 T5242 T5244 aux has,reported
R4461 T5243 T5244 auxpass been,reported
R4462 T5244 T5244 ROOT reported,reported
R4463 T5245 T5248 mark that,activated
R4464 T5246 T5248 nsubjpass NF-κB,activated
R4465 T5247 T5248 auxpass is,activated
R4466 T5248 T5244 ccomp activated,reported
R4467 T5249 T5248 prep in,activated
R4468 T5250 T5249 pobj brain,in
R4469 T5251 T5248 prep after,activated
R4470 T5252 T5255 nmod TBI,]
R4471 T5253 T5255 nmod [,]
R4472 T5254 T5255 nummod 2,]
R4473 T5255 T5251 pobj ],after
R4474 T5256 T5244 punct .,reported
R4475 T5257 T5260 advmod Here,studied
R4476 T5258 T5260 punct ",",studied
R4477 T5259 T5260 nsubj we,studied
R4478 T5260 T5260 ROOT studied,studied
R4479 T5261 T5263 det the,activity
R4480 T5262 T5263 amod DNA-binding,activity
R4481 T5263 T5260 dobj activity,studied
R4482 T5264 T5263 prep of,activity
R4483 T5265 T5264 pobj NF-κB,of
R4484 T5266 T5265 prep of,NF-κB
R4485 T5267 T5266 pobj astrocytes,of
R4486 T5268 T5265 prep from,NF-κB
R4487 T5269 T5270 compound Nrf2,WT
R4488 T5270 T5268 pobj WT,from
R4489 T5271 T5270 cc or,WT
R4490 T5272 T5270 conj KO,WT
R4491 T5273 T5264 pobj mice,of
R4492 T5274 T5260 prep by,studied
R4493 T5275 T5274 pobj EMSA,by
R4494 T5276 T5260 prep at,studied
R4495 T5277 T5278 nummod 24,h
R4496 T5278 T5276 pobj h,at
R4497 T5279 T5260 prep after,studied
R4498 T5280 T5281 compound scratch,injury
R4499 T5281 T5279 pobj injury,after
R4500 T5282 T5260 punct .,studied
R4501 T5283 T5285 advmod Scratch,induced
R4502 T5284 T5285 nsubj injury,induced
R4503 T5285 T5285 ROOT induced,induced
R4504 T5286 T5285 dobj activation,induced
R4505 T5287 T5286 prep of,activation
R4506 T5288 T5287 pobj NF-κB,of
R4507 T5289 T5286 prep in,activation
R4508 T5290 T5289 pobj astrocytes,in
R4509 T5291 T5290 prep of,astrocytes
R4510 T5292 T5293 det both,genotypes
R4511 T5293 T5291 pobj genotypes,of
R4512 T5294 T5285 punct ",",induced
R4513 T5295 T5300 mark while,observed
R4514 T5296 T5298 amod higher,activity
R4515 T5297 T5298 compound NF-κB,activity
R4516 T5298 T5300 nsubjpass activity,observed
R4517 T5299 T5300 auxpass was,observed
R4518 T5300 T5285 advcl observed,induced
R4519 T5301 T5300 prep in,observed
R4520 T5302 T5304 compound group,TBI
R4521 T5303 T5304 compound KO,TBI
R4522 T5304 T5301 pobj TBI,in
R4523 T5305 T5300 prep than,observed
R4524 T5306 T5305 prep in,than
R4525 T5307 T5309 compound group,TBI
R4526 T5308 T5309 compound WT,TBI
R4527 T5309 T5306 pobj TBI,in
R4528 T5310 T5309 punct (,TBI
R4529 T5311 T5312 nummod 2.67,±
R4530 T5312 T5320 dep ±,<
R4531 T5313 T5320 dep 0.173,<
R4532 T5314 T5320 prep versus,<
R4533 T5315 T5316 nummod 2.28,±
R4534 T5316 T5314 pobj ±,versus
R4535 T5317 T5316 appos 0.072,±
R4536 T5318 T5320 punct ",",<
R4537 T5319 T5320 nsubj P,<
R4538 T5320 T5320 ROOT <,<
R4539 T5321 T5320 npadvmod 0.01,<
R4540 T5322 T5320 punct ),<
R4541 T5323 T5324 punct (,Figure
R4542 T5324 T5320 appos Figure,<
R4543 T5325 T5324 nummod 2,Figure
R4544 T5326 T5324 punct ),Figure
R4545 T5327 T5320 punct .,<
R4985 T5965 T5973 punct ",",regulated
R4986 T5966 T5973 nsubjpass TNF-α,regulated
R4987 T5967 T5966 punct ",",TNF-α
R4988 T5968 T5966 conj IL-1β,TNF-α
R4989 T5969 T5968 punct ",",IL-1β
R4990 T5970 T5968 cc and,IL-1β
R4991 T5971 T5968 conj IL-6,IL-1β
R4992 T5972 T5973 auxpass are,regulated
R4993 T5973 T5973 ROOT regulated,regulated
R4994 T5974 T5973 agent by,regulated
R4995 T5975 T5974 pobj NF-κB,by
R4996 T5976 T5973 cc and,regulated
R4997 T5977 T5973 conj reflect,regulated
R4998 T5978 T5980 det the,activity
R4999 T5979 T5980 amod endogenous,activity
R5000 T5980 T5977 dobj activity,reflect
R5001 T5981 T5980 prep of,activity
R5002 T5982 T5981 pobj NF-κB,of
R5003 T5983 T5973 punct .,regulated
R5004 T5984 T5987 nsubjpass It,revealed
R5005 T5985 T5987 aux has,revealed
R5006 T5986 T5987 auxpass been,revealed
R5007 T5987 T5987 ROOT revealed,revealed
R5008 T5988 T5993 mark that,increased
R5009 T5989 T5991 det these,cytokines
R5010 T5990 T5991 amod proinflammatory,cytokines
R5011 T5991 T5993 nsubjpass cytokines,increased
R5012 T5992 T5993 auxpass are,increased
R5013 T5993 T5987 ccomp increased,revealed
R5014 T5994 T5993 prep after,increased
R5015 T5995 T5994 pobj TBI,after
R5016 T5996 T5993 prep in,increased
R5017 T5997 T5996 pobj animals,in
R5018 T5998 T5993 conj [,increased
R5019 T5999 T5998 nummod 2,[
R5020 T6000 T5998 punct ",",[
R5021 T6001 T6002 nummod 8,]
R5022 T6002 T5998 appos ],[
R5023 T6003 T5987 punct .,revealed
R5024 T6004 T6009 cc And,proved
R5025 T6005 T6007 poss our,study
R5026 T6006 T6007 amod previous,study
R5027 T6007 T6009 nsubj study,proved
R5028 T6008 T6009 aux has,proved
R5029 T6009 T6009 ROOT proved,proved
R5030 T6010 T6018 mark that,observed
R5031 T6011 T6012 amod higher,levels
R5032 T6012 T6018 nsubjpass levels,observed
R5033 T6013 T6012 prep of,levels
R5034 T6014 T6016 amod such,cytokines
R5035 T6015 T6016 amod proinflammatory,cytokines
R5036 T6016 T6013 pobj cytokines,of
R5037 T6017 T6018 auxpass were,observed
R5038 T6018 T6009 ccomp observed,proved
R5039 T6019 T6018 prep in,observed
R5040 T6020 T6021 det the,brain
R5041 T6021 T6019 pobj brain,in
R5042 T6022 T6021 prep of,brain
R5043 T6023 T6024 amod Nrf2-deficient,mice
R5044 T6024 T6022 pobj mice,of
R5045 T6025 T6018 prep after,observed
R5046 T6026 T6029 nmod TBI,]
R5047 T6027 T6029 nmod [,]
R5048 T6028 T6029 nummod 5,]
R5049 T6029 T6025 pobj ],after
R5050 T6030 T6009 punct .,proved
R5051 T6031 T6033 cc But,is
R5052 T6032 T6033 nsubj it,is
R5053 T6033 T6033 ROOT is,is
R5054 T6034 T6033 advmod still,is
R5055 T6035 T6033 acomp ambiguous,is
R5056 T6036 T6035 prep about,ambiguous
R5057 T6037 T6038 det the,relationship
R5058 T6038 T6036 pobj relationship,about
R5059 T6039 T6038 prep between,relationship
R5060 T6040 T6041 det these,cytokines
R5061 T6041 T6039 pobj cytokines,between
R5062 T6042 T6041 cc and,cytokines
R5063 T6043 T6041 conj Nrf2,cytokines
R5114 T6094 T6093 prep in,scratch
R5115 T6095 T6096 det both,WT
R5116 T6096 T6094 pobj WT,in
R5117 T6097 T6096 cc and,WT
R5118 T6098 T6096 conj KO,WT
R5119 T6099 T6091 advmod astrocytes,increased
R5120 T6100 T6101 advmod when,compared
R5121 T6101 T6099 advcl compared,astrocytes
R5122 T6102 T6101 prep with,compared
R5123 T6103 T6105 poss their,counterparts
R5124 T6104 T6105 amod sham,counterparts
R5125 T6105 T6102 pobj counterparts,with
R5126 T6106 T6105 punct ",",counterparts
R5127 T6107 T6101 advmod respectively,compared
R5128 T6108 T6078 punct .,shown
R5129 T6109 T6121 advmod Moreover,were
R5130 T6110 T6121 punct ",",were
R5131 T6111 T6113 det the,levels
R5132 T6112 T6113 amod mRNA,levels
R5133 T6113 T6121 nsubj levels,were
R5134 T6114 T6113 prep of,levels
R5135 T6115 T6116 det these,cytokines
R5136 T6116 T6114 pobj cytokines,of
R5137 T6117 T6113 prep in,levels
R5138 T6118 T6117 pobj group,in
R5139 T6119 T6120 compound KO,TBI
R5140 T6120 T6121 nsubj TBI,were
R5141 T6121 T6121 ROOT were,were
R5142 T6122 T6123 advmod much,higher
R5143 T6123 T6121 acomp higher,were
R5144 T6124 T6123 prep than,higher
R5145 T6125 T6124 pobj those,than
R5146 T6126 T6125 prep in,those
R5147 T6127 T6129 compound group,TBI
R5148 T6128 T6129 compound WT,TBI
R5149 T6129 T6126 pobj TBI,in
R5150 T6130 T6129 punct (,TBI
R5151 T6131 T6132 nummod 1.66,±
R5152 T6132 T6137 nummod ±,0.110
R5153 T6133 T6137 nummod 0.085,0.110
R5154 T6134 T6133 prep versus,0.085
R5155 T6135 T6136 nummod 1.39,±
R5156 T6136 T6134 pobj ±,versus
R5157 T6137 T6129 appos 0.110,TBI
R5158 T6138 T6137 prep for,0.110
R5159 T6139 T6138 pobj TNF-α,for
R5160 T6140 T6139 punct ",",TNF-α
R5161 T6141 T6142 nummod 1.34,±
R5162 T6142 T6139 appos ±,TNF-α
R5163 T6143 T6142 conj 0.064,±
R5164 T6144 T6143 prep versus,0.064
R5165 T6145 T6147 nummod 0.73,0.088
R5166 T6146 T6147 nummod ±,0.088
R5167 T6147 T6144 pobj 0.088,versus
R5168 T6148 T6147 prep for,0.088
R5169 T6149 T6148 pobj IL-1β,for
R5170 T6150 T6139 punct ",",TNF-α
R5171 T6151 T6129 cc and,TBI
R5172 T6152 T6153 nummod 1.28,±
R5173 T6153 T6155 npadvmod ±,versus
R5174 T6154 T6155 nummod 0.102,versus
R5175 T6155 T6121 prep versus,were
R5176 T6156 T6158 nummod 0.99,0.073
R5177 T6157 T6158 nummod ±,0.073
R5178 T6158 T6155 pobj 0.073,versus
R5179 T6159 T6158 prep for,0.073
R5180 T6160 T6159 pobj IL-6,for
R5181 T6161 T6121 punct .,were
R5182 T6162 T6163 compound P,<
R5183 T6163 T6163 ROOT <,<
R5184 T6164 T6163 appos 0.01,<
R5185 T6165 T6163 punct ),<
R5186 T6166 T6167 punct (,Figures
R5187 T6167 T6163 appos Figures,<
R5188 T6168 T6167 nummod 3,Figures
R5189 T6169 T6170 punct (,a
R5190 T6170 T6167 parataxis a,Figures
R5191 T6171 T6170 punct ),a
R5192 T6172 T6170 meta 3,a
R5193 T6173 T6174 punct (,d
R5194 T6174 T6172 parataxis d,3
R5195 T6175 T6174 punct ),d
R5196 T6176 T6174 punct ),d
R5197 T6177 T6170 punct .,a
R5198 T6178 T6179 det The,results
R5199 T6179 T6182 nsubj results,revealed
R5200 T6180 T6179 prep of,results
R5201 T6181 T6180 pobj ELISA,of
R5202 T6182 T6182 ROOT revealed,revealed
R5203 T6183 T6189 mark that,was
R5204 T6184 T6186 det the,level
R5205 T6185 T6186 compound protein,level
R5206 T6186 T6189 nsubj level,was
R5207 T6187 T6186 prep of,level
R5208 T6188 T6187 pobj TNF-α,of
R5209 T6189 T6182 ccomp was,revealed
R5210 T6190 T6189 acomp upregulated,was
R5211 T6191 T6190 prep after,upregulated
R5212 T6192 T6193 compound scratch,injury
R5213 T6193 T6191 pobj injury,after
R5214 T6194 T6193 prep in,injury
R5215 T6195 T6194 pobj WT,in
R5216 T6196 T6195 cc and,WT
R5217 T6197 T6195 conj KO,WT
R5218 T6198 T6189 npadvmod astrocytes,was
R5219 T6199 T6200 advmod when,compared
R5220 T6200 T6189 prep compared,was
R5221 T6201 T6200 prep with,compared
R5222 T6202 T6204 poss their,counterparts
R5223 T6203 T6204 amod sham,counterparts
R5224 T6204 T6201 pobj counterparts,with
R5225 T6205 T6182 punct .,revealed
R5226 T6206 T6208 cc And,was
R5227 T6207 T6208 nsubj it,was
R5228 T6208 T6230 ccomp was,<
R5229 T6209 T6208 advmod also,was
R5230 T6210 T6208 advmod higher,was
R5231 T6211 T6208 prep in,was
R5232 T6212 T6214 compound group,TBI
R5233 T6213 T6214 compound KO,TBI
R5234 T6214 T6211 pobj TBI,in
R5235 T6215 T6208 prep than,was
R5236 T6216 T6215 prep in,than
R5237 T6217 T6216 pobj group,in
R5238 T6218 T6219 compound WT,TBI
R5239 T6219 T6217 appos TBI,group
R5240 T6220 T6221 punct (,3.67
R5241 T6221 T6223 nummod 3.67,0.156
R5242 T6222 T6223 nummod ±,0.156
R5243 T6223 T6215 pobj 0.156,than
R5244 T6224 T6208 prep versus,was
R5245 T6225 T6226 nummod 2.31,±
R5246 T6226 T6227 nummod ±,0.087
R5247 T6227 T6224 pobj 0.087,versus
R5248 T6228 T6230 punct ",",<
R5249 T6229 T6230 nsubj P,<
R5250 T6230 T6230 ROOT <,<
R5251 T6231 T6230 npadvmod 0.01,<
R5252 T6232 T6230 punct ),<
R5253 T6233 T6234 punct (,Figure
R5254 T6234 T6237 nmod Figure,a
R5255 T6235 T6234 nummod 4,Figure
R5256 T6236 T6237 punct (,a
R5257 T6237 T6230 parataxis a,<
R5258 T6238 T6237 punct ),a
R5259 T6239 T6237 punct ),a
R5260 T6240 T6230 punct .,<
R5261 T6241 T6242 amod Similar,tendency
R5262 T6242 T6244 nsubjpass tendency,observed
R5263 T6243 T6244 auxpass was,observed
R5264 T6244 T6244 ROOT observed,observed
R5265 T6245 T6244 prep in,observed
R5266 T6246 T6247 compound protein,level
R5267 T6247 T6245 pobj level,in
R5268 T6248 T6247 prep of,level
R5269 T6249 T6248 pobj IL-6,of
R5270 T6250 T6249 cc and,IL-6
R5271 T6251 T6249 conj IL-1β,IL-6
R5286 T6266 T6268 nummod 25.76,0.536
R5287 T6267 T6268 nummod ±,0.536
R5288 T6268 T6265 pobj 0.536,while
R5289 T6269 T6268 prep for,0.536
R5290 T6270 T6269 pobj group,for
R5291 T6271 T6272 compound WT,TBI
R5292 T6272 T6270 appos TBI,group
R5293 T6273 T6274 punct (,P
R5294 T6274 T6272 appos P,TBI
R5295 T6275 T6276 compound <,0.01
R5296 T6276 T6274 nummod 0.01,P
R5297 T6277 T6272 punct ),TBI
R5298 T6278 T6279 punct (,Figure
R5299 T6279 T6268 appos Figure,0.536
R5300 T6280 T6279 nummod 4,Figure
R5301 T6281 T6282 punct (,b
R5302 T6282 T6279 appos b,Figure
R5303 T6283 T6282 punct ),b
R5304 T6284 T6279 punct ),Figure
R5305 T6285 T6257 punct .,was
R5306 T6286 T6290 prep For,was
R5307 T6287 T6286 pobj IL-1β,For
R5308 T6288 T6290 punct ",",was
R5309 T6289 T6290 nsubj it,was
R5310 T6290 T6290 ROOT was,was
R5311 T6291 T6293 nummod 190.75,6.339
R5312 T6292 T6293 nummod ±,6.339
R5313 T6293 T6290 attr 6.339,was
R5314 T6294 T6293 prep for,6.339
R5315 T6295 T6297 compound group,TBI
R5316 T6296 T6297 compound KO,TBI
R5317 T6297 T6294 pobj TBI,for
R5318 T6298 T6301 mark while,5.348
R5319 T6299 T6301 nummod 154.50,5.348
R5320 T6300 T6301 nummod ±,5.348
R5321 T6301 T6290 advcl 5.348,was
R5322 T6302 T6301 prep for,5.348
R5323 T6303 T6302 pobj group,for
R5324 T6304 T6305 compound WT,TBI
R5325 T6305 T6303 appos TBI,group
R5326 T6306 T6307 punct (,P
R5327 T6307 T6305 appos P,TBI
R5328 T6308 T6309 compound <,0.01
R5329 T6309 T6307 nummod 0.01,P
R5330 T6310 T6305 punct ),TBI
R5331 T6311 T6312 punct (,Figure
R5332 T6312 T6315 nmod Figure,c
R5333 T6313 T6312 nummod 4,Figure
R5334 T6314 T6315 punct (,c
R5335 T6315 T6303 appos c,group
R5602 T6765 T6764 pobj MMP9,of
R5603 T6766 T6768 auxpass Were,Enhanced
R5604 T6767 T6768 advmod Greatly,Enhanced
R5605 T6768 T6768 ROOT Enhanced,Enhanced
R5606 T6769 T6768 prep in,Enhanced
R5607 T6770 T6772 compound Nrf2,Astrocytes
R5608 T6771 T6772 compound Knockout,Astrocytes
R5609 T6772 T6769 pobj Astrocytes,in
R5610 T6773 T6777 mark after,is
R5611 T6774 T6776 compound Scratch,MMP9
R5612 T6775 T6776 compound Injury,MMP9
R5613 T6776 T6777 nsubj MMP9,is
R5614 T6777 T6768 advcl is,Enhanced
R5615 T6778 T6780 det an,gelatinase
R5616 T6779 T6780 amod important,gelatinase
R5617 T6780 T6777 attr gelatinase,is
R5618 T6781 T6782 aux to,induce
R5619 T6782 T6780 relcl induce,gelatinase
R5620 T6783 T6782 cc or,induce
R5621 T6784 T6782 conj aggravate,induce
R5622 T6785 T6787 det the,process
R5623 T6786 T6787 compound inflammation,process
R5624 T6787 T6784 dobj process,aggravate
R5625 T6788 T6768 punct .,Enhanced
R5626 T6789 T6795 prep In,elevated
R5627 T6790 T6792 det the,study
R5628 T6791 T6792 amod present,study
R5629 T6792 T6789 pobj study,In
R5630 T6793 T6795 punct ",",elevated
R5631 T6794 T6795 nsubj we,elevated
R5632 T6795 T6795 ROOT elevated,elevated
R5633 T6796 T6797 det the,mRNA
R5634 T6797 T6795 dobj mRNA,elevated
R5635 T6798 T6797 punct ",",mRNA
R5636 T6799 T6800 compound protein,levels
R5637 T6800 T6797 conj levels,mRNA
R5638 T6801 T6800 punct ",",levels
R5639 T6802 T6800 cc and,levels
R5640 T6803 T6800 conj activity,levels
R5641 T6804 T6803 prep of,activity
R5642 T6805 T6804 pobj MMP9,of
R5643 T6806 T6803 prep in,activity
R5644 T6807 T6806 pobj astrocytes,in
R5645 T6808 T6795 prep at,elevated
R5646 T6809 T6810 nummod 24,h
R5647 T6810 T6808 pobj h,at
R5648 T6811 T6795 prep after,elevated
R5649 T6812 T6813 compound scratch,injury
R5650 T6813 T6811 pobj injury,after
R5651 T6814 T6813 prep by,injury
R5652 T6815 T6814 pobj RT-PCR,by
R5653 T6816 T6815 punct ",",RT-PCR
R5654 T6817 T6818 amod western,blot
R5655 T6818 T6815 appos blot,RT-PCR
R5656 T6819 T6815 punct ",",RT-PCR
R5657 T6820 T6815 cc and,RT-PCR
R5658 T6821 T6822 compound gelatine,zymography
R5659 T6822 T6813 conj zymography,injury
R5660 T6823 T6795 punct .,elevated
R5661 T6824 T6828 nsubjpass Expression,elevated
R5662 T6825 T6824 prep of,Expression
R5663 T6826 T6825 pobj MMP9,of
R5664 T6827 T6828 auxpass was,elevated
R5665 T6828 T6828 ROOT elevated,elevated
R5666 T6829 T6828 prep after,elevated
R5667 T6830 T6831 compound scratch,injury
R5668 T6831 T6829 pobj injury,after
R5669 T6832 T6831 prep in,injury
R5670 T6833 T6832 pobj WT,in
R5671 T6834 T6833 cc and,WT
R5672 T6835 T6833 conj KO,WT
R5673 T6836 T6828 npadvmod astrocytes,elevated
R5674 T6837 T6828 prep as,elevated
R5675 T6838 T6837 prep compared,as
R5676 T6839 T6838 prep with,compared
R5677 T6840 T6842 poss their,counterparts
R5678 T6841 T6842 amod sham,counterparts
R5679 T6842 T6839 pobj counterparts,with
R5680 T6843 T6828 punct .,elevated
R5681 T6844 T6846 det The,level
R5682 T6845 T6846 amod mRNA,level
R5683 T6846 T6849 nsubj level,was
R5684 T6847 T6846 prep of,level
R5685 T6848 T6847 pobj MMP9,of
R5686 T6849 T6849 ROOT was,was
R5687 T6850 T6849 acomp higher,was
R5688 T6851 T6849 prep in,was
R5689 T6852 T6854 compound group,TBI
R5690 T6853 T6854 compound KO,TBI
R5691 T6854 T6851 pobj TBI,in
R5692 T6855 T6849 prep than,was
R5693 T6856 T6855 prep in,than
R5761 T6924 T6890 punct .,discovered
R5762 T6925 T6926 compound Gelatine,zymography
R5763 T6926 T6927 nsubj zymography,revealed
R5764 T6927 T6927 ROOT revealed,revealed
R5765 T6928 T6930 amod higher,activity
R5766 T6929 T6930 compound MMP9,activity
R5767 T6930 T6927 dobj activity,revealed
R5768 T6931 T6930 prep in,activity
R5769 T6932 T6934 compound group,TBI
R5770 T6933 T6934 compound KO,TBI
R5771 T6934 T6931 pobj TBI,in
R7091 T8763 T8761 conj IL-1β,TNF-α
R7092 T8764 T8763 punct ",",IL-1β
R7093 T8765 T8763 conj IL-6,IL-1β
R7094 T8766 T8765 punct ",",IL-6
R7095 T8767 T8765 cc and,IL-6
R7096 T8768 T8765 conj MMP9,IL-6
R7097 T8769 T8752 prep in,upregulation
R7098 T8770 T8771 amod cultured,astrocytes
R7099 T8771 T8769 pobj astrocytes,in
R7100 T8772 T8746 punct .,demonstrated
R7101 T8773 T8775 advmod Also,revealed
R7102 T8774 T8775 nsubj we,revealed
R7103 T8775 T8775 ROOT revealed,revealed
R7104 T8776 T8779 mark that,induced
R7105 T8777 T8778 compound scratch,injury
R7106 T8778 T8779 nsubj injury,induced
R7107 T8779 T8775 ccomp induced,revealed
R7108 T8780 T8781 amod higher,activity
R7109 T8781 T8779 dobj activity,induced
R7110 T8782 T8781 prep of,activity
R7111 T8783 T8782 pobj NF-κB,of
R7112 T8784 T8781 cc and,activity
R7113 T8785 T8786 amod enhanced,expression
R7114 T8786 T8781 conj expression,activity
R7115 T8787 T8786 prep of,expression
R7116 T8788 T8789 amod proinflammatory,cytokines
R7117 T8789 T8787 pobj cytokines,of
R7118 T8790 T8789 prep in,cytokines
R7119 T8791 T8792 amod Nrf2,knockout
R7120 T8792 T8790 pobj knockout,in
R7121 T8793 T8779 conj cultured,induced
R7122 T8794 T8793 dobj astrocytes,cultured
R7123 T8795 T8794 prep than,astrocytes
R7124 T8796 T8795 pobj those,than
R7125 T8797 T8796 prep in,those
R7126 T8798 T8799 compound wildtype,astrocytes
R7127 T8799 T8797 pobj astrocytes,in
R7128 T8800 T8793 prep for,cultured
R7129 T8801 T8803 det the,time
R7130 T8802 T8803 amod first,time
R7131 T8803 T8800 pobj time,for
R7132 T8804 T8775 punct .,revealed
R7133 T8805 T8808 nsubjpass It,demonstrated
R7134 T8806 T8808 aux has,demonstrated
R7135 T8807 T8808 auxpass been,demonstrated
R7136 T8808 T8808 ROOT demonstrated,demonstrated
R7137 T8809 T8812 mark that,activated
R7138 T8810 T8812 nsubjpass NF-κB,activated
R7139 T8811 T8812 auxpass is,activated
R7140 T8812 T8808 ccomp activated,demonstrated
R7141 T8813 T8812 prep in,activated
R7142 T8814 T8813 pobj brain,in
R7143 T8815 T8814 cc and,brain
R7144 T8816 T8817 amod spinal,cord
R7145 T8817 T8814 conj cord,brain
R7146 T8818 T8812 prep after,activated
R7147 T8819 T8821 amod traumatic,[
R7148 T8820 T8821 compound injury,[
R7149 T8821 T8818 pobj [,after
R7150 T8822 T8821 nummod 2,[
R7167 T8839 T8839 ROOT activated,activated
R7168 T8840 T8839 cc and,activated
R7169 T8841 T8839 conj induces,activated
R7170 T8842 T8841 dobj transcription,induces
R7171 T8843 T8842 prep of,transcription
R7172 T8844 T8843 pobj MMP9,of
R7173 T8845 T8842 cc and,transcription
R7174 T8846 T8847 det a,battery
R7175 T8847 T8842 conj battery,transcription
R7176 T8848 T8847 prep of,battery
R7177 T8849 T8850 amod proinflammatory,cytokines
R7178 T8850 T8848 pobj cytokines,of
R7179 T8851 T8850 punct ",",cytokines
R7180 T8852 T8850 prep including,cytokines
R7181 T8853 T8852 pobj TNF-α,including
R7182 T8854 T8853 punct ",",TNF-α
R7183 T8855 T8853 conj IL-1β,TNF-α
R7184 T8856 T8855 punct ",",IL-1β
R7185 T8857 T8855 cc and,IL-1β
R7186 T8858 T8855 conj IL-6,IL-1β
R7187 T8859 T8850 punct ",",cytokines
R7188 T8860 T8847 prep in,battery
R7189 T8861 T8862 compound brain,tissues
R7190 T8862 T8860 pobj tissues,in
R7191 T8863 T8841 prep after,induces
R7192 T8864 T8865 compound TBI,[
R7193 T8865 T8863 pobj [,after
R7194 T8866 T8868 quantmod 3,10
R7195 T8867 T8868 punct ",",10
R7196 T8868 T8871 nummod 10,]
R7197 T8869 T8870 punct ",",11
R7198 T8870 T8871 nummod 11,]
R7199 T8871 T8865 appos ],[
R7200 T8872 T8839 punct .,activated
R7201 T8873 T8874 poss Our,results
R7202 T8874 T8876 nsubj results,revealed
R7203 T8875 T8876 advmod also,revealed
R7204 T8876 T8876 ROOT revealed,revealed
R7205 T8877 T8880 mark that,induced
R7206 T8878 T8879 compound scratch,injury
R7207 T8879 T8880 nsubj injury,induced
R7208 T8880 T8876 ccomp induced,revealed
R7209 T8881 T8880 dobj elevation,induced
R7210 T8882 T8881 prep of,elevation
R7211 T8883 T8884 compound NF-κB,DNA
R7212 T8884 T8882 pobj DNA,of
R7213 T8885 T8886 amod binding,activity
R7214 T8886 T8880 conj activity,induced
R7215 T8887 T8886 punct ",",activity
R7216 T8888 T8886 conj overexpression,activity
R7217 T8889 T8888 prep of,overexpression
R7218 T8890 T8889 pobj MMP9,of
R7219 T8891 T8888 punct ",",overexpression
R7220 T8892 T8888 cc and,overexpression
R7221 T8893 T8894 amod proinflammatory,mediators
R7222 T8894 T8888 conj mediators,overexpression
R7223 T8895 T8894 acl mentioned,mediators
R7224 T8896 T8895 advmod above,mentioned
R7225 T8897 T8895 prep in,mentioned
R7226 T8898 T8899 amod cultured,astrocytes
R7227 T8899 T8897 pobj astrocytes,in
R7228 T8900 T8876 punct .,revealed
R7248 T8920 T8919 cc and,TNF-α
R7249 T8921 T8919 conj IL-1β,TNF-α
R7250 T8922 T8922 ROOT are,are
R7251 T8923 T8924 amod potent,stimulators
R7252 T8924 T8922 attr stimulators,are
R7253 T8925 T8924 prep for,stimulators
R7254 T8926 T8925 pobj MMP9,for
R7255 T8927 T8924 prep in,stimulators
R7256 T8928 T8927 pobj astrocytes,in
R7257 T8929 T8933 nmod [,]
R7258 T8930 T8933 nummod 12,]
R7259 T8931 T8933 punct ",",]
R7260 T8932 T8933 nummod 13,]
R7261 T8933 T8937 nsubj ],induces
R7262 T8934 T8933 punct ",",]
R7263 T8935 T8933 cc and,]
R7264 T8936 T8933 conj IL-6,]
R7265 T8937 T8937 ROOT induces,induces
R7266 T8938 T8937 dobj overexpression,induces
R7267 T8939 T8938 prep of,overexpression
R7268 T8940 T8939 pobj MMP9,of
R7269 T8941 T8938 prep in,overexpression
R7270 T8942 T8944 amod human,carcinoma
R7271 T8943 T8944 compound colon,carcinoma
R7272 T8944 T8945 compound carcinoma,cells
R7273 T8945 T8941 pobj cells,in
R7274 T8946 T8948 nmod [,]
R7275 T8947 T8948 nummod 14,]
R7276 T8948 T8937 dobj ],induces
R7277 T8949 T8948 punct ",",]
R7278 T8950 T8948 cc and,]
R7279 T8951 T8948 conj TNF-α,]
R7280 T8952 T8951 cc and,TNF-α
R7281 T8953 T8951 conj IL-1β,TNF-α
R7282 T8954 T8956 advmod also,induce
R7283 T8955 T8956 aux can,induce
R7284 T8956 T8937 conj induce,induces
R7285 T8957 T8956 dobj activation,induce
R7286 T8958 T8957 prep of,activation
R7287 T8959 T8960 compound NF-κB,[
R7288 T8960 T8958 pobj [,of
R7289 T8961 T8960 nummod 15,[
R7290 T8962 T8960 punct ",",[
R7291 T8963 T8964 nummod 16,]
R7292 T8964 T8960 appos ],[
R7293 T8965 T8937 punct .,induces
R7294 T8966 T8968 det This,loop
R7295 T8967 T8968 amod autoregulatory,loop
R7296 T8968 T8970 nsubj loop,aggravates
R7297 T8969 T8970 advmod extremely,aggravates
R7298 T8970 T8970 ROOT aggravates,aggravates
R7299 T8971 T8973 det the,effect
R7300 T8972 T8973 amod damaging,effect
R7301 T8973 T8970 dobj effect,aggravates
R7302 T8974 T8973 prep of,effect
R7303 T8975 T8974 pobj inflammation,of
R7304 T8976 T8970 cc and,aggravates
R7305 T8977 T8970 conj induces,aggravates
R7306 T8978 T8979 amod secondary,injury
R7307 T8979 T8977 dobj injury,induces
R7327 T8998 T8995 dobj loop,prevent
R7328 T9026 T9029 nsubjpass which,involved
R7329 T9027 T9029 auxpass are,involved
R7330 T8999 T8998 prep after,loop
R7331 T9028 T9029 dep all,involved
R7332 T9029 T9024 relcl involved,metabolites
R7333 T9000 T8999 pobj TBI,after
R7334 T9030 T9029 prep in,involved
R7335 T9031 T9030 pobj TBI,in
R7336 T9001 T8984 punct .,is
R7337 T9032 T9006 punct .,proved
R7338 T9033 T9035 poss Our,study
R7339 T9002 T9003 amod Nrf2-ARE,pathway
R7340 T9034 T9035 amod previous,study
R7341 T9035 T9037 nsubj study,proved
R7342 T9036 T9037 aux has,proved
R7343 T9003 T9006 nsubjpass pathway,proved
R7344 T9037 T9037 ROOT proved,proved
R7345 T9038 T9039 det the,augmentation
R7346 T9039 T9037 dobj augmentation,proved
R7347 T9004 T9006 aux has,proved
R7348 T9040 T9039 prep of,augmentation
R7349 T9041 T9040 pobj Nrf2,of
R7350 T9042 T9039 prep in,augmentation
R7351 T9005 T9006 auxpass been,proved
R7352 T9043 T9044 compound brain,tissue
R7353 T9044 T9042 pobj tissue,in
R7354 T9045 T9037 prep after,proved
R7355 T9006 T9006 ROOT proved,proved
R7356 T9046 T9047 compound TBI,[
R7357 T9047 T9045 pobj [,after
R7358 T9007 T9008 aux to,be
R7359 T9048 T9047 nummod 17,[
R7360 T9049 T9047 conj ],[
R7361 T9008 T9006 xcomp be,proved
R7362 T9050 T9037 punct .,proved
R7363 T9051 T9052 amod Enhanced,level
R7364 T9052 T9052 ROOT level,level
R7365 T9009 T9011 det the,regulator
R7366 T9053 T9052 prep of,level
R7367 T9010 T9011 amod key,regulator
R7368 T9054 T9055 amod Nrf2,activates
R7369 T9055 T9053 pobj activates,of
R7370 T9056 T9055 dobj transcription,activates
R7371 T9011 T9008 attr regulator,be
R7374 T9012 T9011 prep in,regulator
R7375 T9013 T9012 pcomp reducing,in
R7376 T9014 T9015 amod oxidative,stress
R7377 T9015 T9013 dobj stress,reducing
R7378 T9016 T9015 punct ",",stress
R7381 T9017 T9018 amod inflammatory,damage
R7385 T9018 T9015 conj damage,stress
R7388 T9019 T9018 punct ",",damage
R7390 T9020 T9018 cc and,damage
R7391 T9021 T9018 conj accumulation,damage
R7394 T9022 T9021 prep of,accumulation
R7398 T9023 T9024 amod toxic,metabolites
R7401 T9024 T9022 pobj metabolites,of
R7407 T9078 T9079 compound quinone,oxidoreductase-1
R7409 T9079 T9052 appos oxidoreductase-1,level
R7410 T9080 T9079 punct (,oxidoreductase-1
R7411 T9081 T9079 appos NQO1,oxidoreductase-1
R7413 T9082 T9079 punct ),oxidoreductase-1
R7414 T9083 T9052 punct ",",level
R7415 T9084 T9087 nsubj which,reduce
R7417 T9085 T9087 aux would,reduce
R7418 T9086 T9087 advmod subsequently,reduce
R7420 T9087 T9052 relcl reduce,level
R7421 T9088 T9089 det the,damage
R7422 T9089 T9087 dobj damage,reduce
R7423 T9090 T9089 prep in,damage
R7424 T9091 T9090 pobj brain,in
R7426 T9092 T9094 nmod [,]
R7427 T9093 T9094 nummod 18,]
R7428 T9094 T9089 appos ],damage
R7429 T9095 T9052 punct .,level
R7430 T9096 T9097 compound Postinjury,administration
R7432 T9097 T9107 nsubj administration,improves
R7433 T9098 T9097 prep of,administration
R7434 T9099 T9098 pobj SFN,of
R7436 T9100 T9099 punct ",",SFN
R7438 T9101 T9102 det an,inducer
R7439 T9102 T9099 appos inducer,SFN
R7441 T9103 T9102 prep of,inducer
R7442 T9104 T9103 pobj Nrf2,of
R7443 T9105 T9107 punct ",",improves
R7444 T9166 T9165 pobj brain,in
R7445 T9106 T9107 advmod significantly,improves
R7446 T9107 T9107 ROOT improves,improves
R7447 T9108 T9109 amod spatial,memory
R7448 T9167 T9159 prep after,induces
R7449 T9109 T9107 dobj memory,improves
R7450 T9110 T9109 prep of,memory
R7451 T9111 T9110 pobj rat,of
R7452 T9168 T9169 compound TBI,[
R7453 T9112 T9107 cc and,improves
R7454 T9113 T9107 conj decreases,improves
R7455 T9114 T9115 det the,immunoreactivity
R7456 T9169 T9171 nmod [,]
R7457 T9115 T9113 dobj immunoreactivity,decreases
R7458 T9116 T9115 prep for,immunoreactivity
R7459 T9117 T9116 pobj 4-Hydroxynonenal,for
R7460 T9170 T9171 nummod 20,]
R7461 T9118 T9117 punct (,4-Hydroxynonenal
R7462 T9119 T9117 appos 4-HNE,4-Hydroxynonenal
R7463 T9120 T9117 punct ),4-Hydroxynonenal
R7464 T9171 T9167 pobj ],after
R7465 T9121 T9115 punct ",",immunoreactivity
R7466 T9122 T9123 det a,marker
R7467 T9123 T9115 appos marker,immunoreactivity
R7468 T9172 T9159 punct .,induces
R7469 T9124 T9123 prep of,marker
R7470 T9125 T9126 amod lipid,peroxidation
R7471 T9126 T9124 pobj peroxidation,of
R7472 T9173 T9177 advmod Here,revealed
R7473 T9127 T9113 punct ",",decreases
R7474 T9128 T9113 prep in,decreases
R7475 T9129 T9130 det the,cortex
R7476 T9130 T9128 pobj cortex,in
R7477 T9131 T9130 cc and,cortex
R7478 T9132 T9134 det the,subfield
R7479 T9174 T9177 punct ",",revealed
R7480 T9133 T9134 compound CA3,subfield
R7481 T9134 T9130 conj subfield,cortex
R7482 T9175 T9176 poss our,results
R7483 T9176 T9177 nsubj results,revealed
R7485 T9177 T9177 ROOT revealed,revealed
R7488 T9178 T9192 mark that,was
R7491 T9179 T9192 nsubj overexpression,was
R7495 T9180 T9179 prep of,overexpression
R7498 T9181 T9180 pobj TNF-α,of
R7499 T9182 T9181 punct ",",TNF-α
R7500 T9145 T9146 amod other,hand
R7501 T9146 T9143 pobj hand,On
R7502 T9183 T9181 conj IL-1β,TNF-α
R7503 T9147 T9150 punct ",",appear
R7504 T9148 T9149 amod Nrf2-deficient,mice
R7505 T9184 T9183 punct ",",IL-1β
R7506 T9149 T9150 nsubj mice,appear
R7507 T9150 T9150 ROOT appear,appear
R7508 T9185 T9183 conj IL-6,IL-1β
R7509 T9151 T9152 advmod more,susceptible
R7510 T9152 T9150 oprd susceptible,appear
R7511 T9153 T9152 prep to,susceptible
R7512 T9186 T9185 punct ",",IL-6
R7513 T9154 T9153 pobj TBI,to
R7514 T9155 T9150 punct .,appear
R7515 T9187 T9185 cc and,IL-6
R7516 T9188 T9185 conj MMP9,IL-6
R7517 T9189 T9179 prep after,overexpression
R7518 T9190 T9191 compound scratch,injury
R7519 T9191 T9189 pobj injury,after
R7520 T9192 T9194 auxpass was,aggravated
R7521 T9253 T9253 ROOT found,found
R7522 T9193 T9194 advmod more,aggravated
R7523 T9254 T9255 aux to,be
R7524 T9255 T9253 xcomp be,found
R7525 T9256 T9255 acomp able,be
R7526 T9257 T9258 aux to,inhibit
R7527 T9258 T9256 xcomp inhibit,able
R7528 T9194 T9177 ccomp aggravated,revealed
R7529 T9259 T9261 compound IKK/I,phosphorylation
R7530 T9260 T9261 compound κB,phosphorylation
R7531 T9261 T9258 dobj phosphorylation,inhibit
R7532 T9195 T9194 prep in,aggravated
R7533 T9262 T9261 cc and,phosphorylation
R7534 T9263 T9267 nummod p65,translocation
R7535 T9196 T9199 amod cultured,astrocytes
R7536 T9264 T9267 amod NF-κB,translocation
R7537 T9265 T9267 nmod subunit,translocation
R7538 T9197 T9199 amod Nrf2,astrocytes
R7539 T9266 T9267 amod nuclear,translocation
R7540 T9267 T9261 conj translocation,phosphorylation
R7541 T9198 T9199 compound knockout,astrocytes
R7542 T9268 T9253 punct ",",found
R7543 T9269 T9270 advmod consequently,alleviating
R7544 T9270 T9253 advcl alleviating,found
R7545 T9199 T9195 pobj astrocytes,in
R7546 T9271 T9270 dobj NF-κB,alleviating
R7547 T9272 T9271 csubj signaling,NF-κB
R7548 T9273 T9275 nmod [,]
R7549 T9200 T9194 prep than,aggravated
R7550 T9274 T9275 nummod 21,]
R7551 T9275 T9272 dobj ],signaling
R7552 T9276 T9253 punct .,found
R7553 T9201 T9200 prep in,than
R7554 T9277 T9288 cc And,attenuated
R7555 T9278 T9279 amod NF-κB,activation
R7556 T9279 T9288 nsubjpass activation,attenuated
R7557 T9202 T9203 compound wildtype,astrocyetes
R7558 T9280 T9279 acl induced,activation
R7559 T9281 T9280 agent by,induced
R7560 T9282 T9281 pobj lipopolysaccharide,by
R7561 T9203 T9201 pobj astrocyetes,in
R7562 T9204 T9194 prep for,aggravated
R7563 T9205 T9207 det the,time
R7564 T9283 T9284 punct (,LPS
R7565 T9206 T9207 amod first,time
R7566 T9284 T9282 appos LPS,lipopolysaccharide
R7567 T9207 T9204 pobj time,for
R7568 T9285 T9282 punct ),lipopolysaccharide
R7569 T9286 T9288 aux could,attenuated
R7570 T9208 T9194 punct ",",aggravated
R7571 T9287 T9288 auxpass be,attenuated
R7572 T9288 T9288 ROOT attenuated,attenuated
R7573 T9289 T9288 agent by,attenuated
R7574 T9290 T9292 amod diverse,activators
R7575 T9291 T9292 amod Nrf2,activators
R7576 T9292 T9289 pobj activators,by
R7577 T9209 T9194 cc and,aggravated
R7578 T9293 T9292 punct ",",activators
R7579 T9294 T9295 amod such,as
R7580 T9295 T9292 prep as,activators
R7581 T9296 T9295 pobj SFN,as
R7582 T9210 T9215 nsubj overexpression,led
R7583 T9297 T9296 cc and,SFN
R7584 T9298 T9302 nmod curcumin,[
R7585 T9211 T9210 prep of,overexpression
R7586 T9299 T9300 punct (,CUR
R7587 T9300 T9298 appos CUR,curcumin
R7588 T9301 T9302 punct ),[
R7589 T9212 T9214 det these,mediators
R7590 T9213 T9214 amod proinflammatory,mediators
R7591 T9302 T9296 conj [,SFN
R7593 T9303 T9304 nummod 22,]
R7595 T9304 T9296 conj ],SFN
R7596 T9305 T9288 punct .,attenuated
R7597 T9306 T9311 advmod Furthermore,indicate
R7599 T9307 T9311 punct ",",indicate
R7600 T9308 T9310 poss our,studies
R7601 T9309 T9310 amod previous,studies
R7603 T9310 T9311 nsubj studies,indicate
R7605 T9311 T9311 ROOT indicate,indicate
R7606 T9312 T9316 mark that,induces
R7607 T9313 T9316 nsubj depletion,induces
R7609 T9314 T9313 prep of,depletion
R7610 T9315 T9314 pobj Nrf2,of
R7612 T9316 T9311 ccomp induces,indicate
R7613 T9317 T9316 dobj augmentation,induces
R7614 T9318 T9317 prep of,augmentation
R7616 T9319 T9320 amod NF-κB,activity
R7617 T9320 T9318 pobj activity,of
R7618 T9321 T9320 cc and,activity
R7620 T9322 T9323 amod inflammatory,response
R7621 T9323 T9320 conj response,activity
R7622 T9324 T9323 prep in,response
R7624 T9325 T9324 pobj lung,in
R7625 T9326 T9325 punct ",",lung
R7626 T9327 T9325 conj brain,lung
R7627 T9328 T9327 punct ",",brain
R7628 T9329 T9323 cc and,response
R7629 T9224 T9225 compound animal,studies
R7630 T9330 T9316 oprd intestine,induces
R7631 T9331 T9316 prep after,induces
R7632 T9332 T9333 compound TBI,[
R7633 T9225 T9223 pobj studies,from
R7634 T9333 T9331 pobj [,after
R7635 T9334 T9333 nummod 4,[
R7636 T9335 T9333 punct ",",[
R7637 T9336 T9333 appos 5,[
R7638 T9226 T9222 conj suggest,obtained
R7639 T9337 T9333 punct ",",[
R7640 T9338 T9339 nummod 23,]
R7641 T9227 T9228 det the,possibility
R7642 T9339 T9333 appos ],[
R7643 T9340 T9311 punct .,indicate
R7644 T9341 T9346 nsubj Results,confirmed
R7645 T9228 T9226 dobj possibility,suggest
R7646 T9342 T9341 prep from,Results
R7647 T9343 T9344 det this,study
R7648 T9229 T9236 mark that,achieved
R7649 T9344 T9342 pobj study,from
R7650 T9345 T9346 advmod further,confirmed
R7651 T9346 T9346 ROOT confirmed,confirmed
R7652 T9230 T9231 amod antioxidant,effect
R7653 T9347 T9348 amod such,relationship
R7654 T9348 T9349 nsubj relationship,existed
R7655 T9349 T9346 ccomp existed,confirmed
R7656 T9231 T9236 nsubjpass effect,achieved
R7657 T9232 T9231 prep of,effect
R7658 T9233 T9232 pobj Nrf2,of
R7659 T9234 T9236 aux may,achieved
R7660 T9350 T9349 prep in,existed
R7661 T9235 T9236 auxpass be,achieved
R7662 T9351 T9352 amod cultured,astrocytes
R7663 T9352 T9350 pobj astrocytes,in
R7664 T9236 T9228 acl achieved,possibility
R7665 T9353 T9349 prep after,existed
R7666 T9354 T9355 compound scratch,injury
R7667 T9237 T9236 agent by,achieved
R7668 T9355 T9353 pobj injury,after
R7669 T9356 T9346 punct .,confirmed
R7670 T9357 T9358 amod Enhanced,activation
R7671 T9238 T9237 pobj suppression,by
R7672 T9358 T9363 nsubjpass activation,discovered
R7673 T9359 T9358 prep of,activation
R7674 T9360 T9359 pobj NF-κB,of
R7675 T9361 T9363 auxpass is,discovered
R7676 T9362 T9363 advmod also,discovered
R7677 T9363 T9363 ROOT discovered,discovered
R7678 T9364 T9363 prep in,discovered
R7679 T9239 T9238 prep of,suppression
R7680 T9365 T9364 pobj lung,in
R7681 T9366 T9364 punct ",",in
R7682 T9367 T9364 conj macrophages,in
R7683 T9240 T9241 amod proinflammatory,pathways
R7684 T9368 T9367 punct ",",macrophages
R7685 T9369 T9367 cc and,macrophages
R7686 T9370 T9372 amod mouse,fibroblasts
R7687 T9241 T9239 pobj pathways,of
R7688 T9371 T9372 amod embryonic,fibroblasts
R7689 T9372 T9367 conj fibroblasts,macrophages
R7690 T9242 T9244 nsubjpass which,mediated
R7691 T9373 T9372 prep of,fibroblasts
R7692 T9374 T9375 amod Nrf2-deficient,mice
R7693 T9375 T9373 pobj mice,of
R7694 T9243 T9244 auxpass are,mediated
R7695 T9376 T9363 prep after,discovered
R7696 T9377 T9378 amod experimental,sepsis
R7697 T9244 T9241 relcl mediated,pathways
R7698 T9378 T9376 pobj sepsis,after
R7699 T9379 T9381 nmod [,]
R7700 T9245 T9244 agent by,mediated
R7701 T9380 T9381 nummod 24,]
R7702 T9246 T9247 amod NF-κB,signaling
R7703 T9247 T9245 pcomp signaling,by
R7706 T9248 T9222 punct .,obtained
R7710 T9249 T9253 nsubjpass Administration,found
R7714 T9250 T9249 prep of,Administration
R7716 T9251 T9250 pobj SFN,of
R7717 T9390 T9391 aux can,inhibit
R7718 T9391 T9387 ccomp inhibit,reported
R7719 T9392 T9391 dobj Nrf2,inhibit
R7720 T9252 T9253 auxpass is,found
R7721 T9393 T9391 prep at,inhibit
R7722 T9394 T9395 amod transcriptional,level
R7723 T9411 T9413 det the,domain
R7724 T9395 T9393 pobj level,at
R7725 T9396 T9387 punct .,reported
R7726 T9397 T9399 nmod NF-κB,subunit
R7727 T9398 T9397 nummod p65,NF-κB
R7728 T9399 T9400 nsubj subunit,repressed
R7729 T9400 T9400 ROOT repressed,repressed
R7730 T9401 T9403 det the,pathway
R7731 T9412 T9413 compound CH1-KIX,domain
R7732 T9402 T9403 amod Nrf2-ARE,pathway
R7733 T9403 T9400 dobj pathway,repressed
R7734 T9404 T9400 prep at,repressed
R7735 T9413 T9410 pobj domain,with
R7736 T9405 T9406 amod transcriptional,level
R7737 T9406 T9404 pobj level,at
R7738 T9407 T9400 prep by,repressed
R7739 T9408 T9409 amod competitive,interaction
R7740 T9414 T9413 prep of,domain
R7741 T9409 T9407 pobj interaction,by
R7742 T9410 T9409 prep with,interaction
R7743 T9415 T9414 pobj CBP,of
R7744 T9416 T9415 cc or,CBP
R7745 T9417 T9419 amod local,hypoacetylation
R7746 T9449 T9446 punct ),kinase
R7747 T9418 T9419 compound histone,hypoacetylation
R7748 T9450 T9427 conj pathway,indicate
R7749 T9419 T9420 compound hypoacetylation,[
R7750 T9420 T9415 conj [,CBP
R7753 T9421 T9422 nummod 25,]
R7756 T9422 T9420 appos ],[
R7759 T9423 T9400 punct .,repressed
R7763 T9424 T9426 predet All,findings
R7765 T9425 T9426 det these,findings
R7766 T9426 T9427 nsubj findings,indicate
R7769 T9427 T9427 ROOT indicate,indicate
R7772 T9428 T9431 det the,crosstalk
R7778 T9429 T9431 amod potential,crosstalk
R7780 T9471 T9472 nummod 18,]
R7781 T9472 T9470 appos ],[
R7782 T9430 T9431 compound complicate,crosstalk
R7783 T9473 T9458 punct .,confirmed
R7784 T9474 T9496 prep In,reasoned
R7785 T9475 T9474 pobj view,In
R7786 T9431 T9427 dobj crosstalk,indicate
R7787 T9476 T9475 prep of,view
R7788 T9477 T9478 det the,fact
R7789 T9432 T9431 prep between,crosstalk
R7790 T9478 T9476 pobj fact,of
R7791 T9479 T9481 mark that,is
R7792 T9480 T9481 nsubj Nrf2,is
R7793 T9433 T9432 pobj NF-κB,between
R7794 T9481 T9478 acl is,fact
R7795 T9482 T9484 det a,factor
R7796 T9483 T9484 compound transcription,factor
R7797 T9434 T9433 cc and,NF-κB
R7798 T9484 T9481 attr factor,is
R7799 T9485 T9487 nsubj which,take
R7800 T9435 T9433 conj Nrf2,NF-κB
R7801 T9486 T9487 aux should,take
R7802 T9487 T9484 relcl take,factor
R7803 T9488 T9487 dobj function,take
R7804 T9436 T9433 punct ",",NF-κB
R7805 T9489 T9490 advmod mainly,in
R7806 T9490 T9487 prep in,take
R7807 T9437 T9440 nsubjpass which,regulated
R7808 T9491 T9490 pobj nucleus,in
R7809 T9492 T9496 punct ",",reasoned
R7810 T9493 T9496 nsubjpass it,reasoned
R7811 T9438 T9440 aux may,regulated
R7812 T9494 T9496 aux can,reasoned
R7813 T9495 T9496 auxpass be,reasoned
R7814 T9496 T9496 ROOT reasoned,reasoned
R7815 T9439 T9440 auxpass be,regulated
R7816 T9497 T9500 mark that,be
R7817 T9498 T9500 nsubj astrocytes,be
R7818 T9499 T9500 aux may,be
R7819 T9440 T9431 relcl regulated,crosstalk
R7820 T9500 T9496 ccomp be,reasoned
R7821 T9501 T9502 nummod one,kind
R7822 T9441 T9440 agent by,regulated
R7823 T9502 T9500 attr kind,be
R7824 T9503 T9502 prep of,kind
R7825 T9504 T9505 compound respondent,cells
R7826 T9505 T9503 pobj cells,of
R7827 T9506 T9502 prep in,kind
R7828 T9507 T9506 pobj activation,in
R7829 T9442 T9446 det the,kinase
R7830 T9508 T9507 prep of,activation
R7831 T9509 T9510 compound Nrf2-ARE,pathway
R7832 T9510 T9508 pobj pathway,of
R7833 T9511 T9500 prep after,be
R7834 T9443 T9446 amod upstream,kinase
R7835 T9512 T9511 pobj TBI,after
R7836 T9513 T9496 punct .,reasoned
R7837 T9444 T9445 amod mitogen-activated,protein
R7838 T9514 T9515 amod Previous,study
R7839 T9515 T9516 nsubj study,identified
R7840 T9445 T9446 compound protein,kinase
R7841 T9516 T9516 ROOT identified,identified
R7842 T9517 T9518 det the,expression
R7843 T9518 T9516 dobj expression,identified
R7844 T9446 T9441 pobj kinase,by
R7845 T9519 T9518 prep of,expression
R7846 T9520 T9519 pobj TNF-α,of
R7847 T9447 T9448 punct (,MAPKs
R7848 T9521 T9520 punct ",",TNF-α
R7849 T9522 T9520 conj IL-1β,TNF-α
R7850 T9523 T9522 punct ",",IL-1β
R7851 T9448 T9446 appos MAPKs,kinase
R7852 T9524 T9522 cc and,IL-1β
R7853 T9525 T9522 conj IL-6,IL-1β
R7854 T9526 T9516 prep in,identified
R7855 T9546 T9549 amod middle,occlusion
R7856 T9527 T9528 amod cultured,astrocytes
R7857 T9528 T9526 pobj astrocytes,in
R7858 T9529 T9516 prep after,identified
R7859 T9547 T9549 amod cerebral,occlusion
R7860 T9548 T9549 compound artery,occlusion
R7861 T9549 T9545 pobj occlusion,after
R7864 T9550 T9554 punct ",",observed
R7867 T9551 T9552 amod MMP9-positive,astrocytes
R7871 T9552 T9554 nsubjpass astrocytes,observed
R7874 T9553 T9554 auxpass were,observed
R7875 T9554 T9543 ccomp observed,revealed
R7876 T9539 T9535 appos ],[
R7877 T9540 T9516 punct .,identified
R7878 T9541 T9542 det Another,study
R7879 T9542 T9543 nsubj study,revealed
R7880 T9543 T9543 ROOT revealed,revealed
R7881 T9555 T9554 prep in,observed
R7882 T9544 T9554 mark that,observed
R7883 T9545 T9554 prep after,observed
R7884 T9556 T9557 compound brain,tissues
R7885 T9557 T9555 pobj tissues,in
R7886 T9558 T9554 agent by,observed
R7887 T9559 T9560 compound immunohistochemistry,[
R7888 T9560 T9558 pobj [,by
R7889 T9561 T9562 nummod 29,]
R7890 T9643 T9642 pobj TNF-α,of
R7891 T9562 T9560 appos ],[
R7892 T9644 T9643 punct ",",TNF-α
R7893 T9645 T9643 conj IL-1β,TNF-α
R7894 T9563 T9543 punct .,revealed
R7895 T9646 T9645 punct ",",IL-1β
R7896 T9647 T9645 conj IL-6,IL-1β
R7897 T9648 T9647 punct ",",IL-6
R7898 T9564 T9565 det Those,results
R7899 T9565 T9566 nsubj results,indicate
R7900 T9566 T9566 ROOT indicate,indicate
R7901 T9649 T9647 cc and,IL-6
R7902 T9567 T9568 det the,role
R7903 T9650 T9647 conj MMP9,IL-6
R7904 T9651 T9634 advcl resulting,induced
R7905 T9568 T9566 dobj role,indicate
R7906 T9652 T9651 prep in,resulting
R7907 T9653 T9655 amod more,deaths
R7908 T9569 T9568 prep of,role
R7909 T9654 T9655 compound cell,deaths
R7910 T9655 T9652 pobj deaths,in
R7911 T9656 T9655 prep in,deaths
R7912 T9570 T9569 pobj astrocytes,of
R7913 T9657 T9656 pobj astrocytes,in
R7914 T9658 T9651 prep after,resulting
R7915 T9571 T9570 prep in,astrocytes
R7916 T9659 T9660 compound scratch,injury
R7917 T9660 T9658 pobj injury,after
R7918 T9661 T9634 punct .,induced
R7919 T9572 T9573 amod inflammatory,process
R7920 T9662 T9663 det These,results
R7921 T9663 T9664 nsubj results,suggest
R7922 T9573 T9571 pobj process,in
R7923 T9664 T9664 ROOT suggest,suggest
R7924 T9665 T9668 mark that,be
R7925 T9574 T9568 prep after,role
R7926 T9666 T9668 nsubj Nrf2,be
R7927 T9667 T9668 aux may,be
R7928 T9668 T9664 ccomp be,suggest
R7929 T9669 T9671 det an,target
R7930 T9670 T9671 amod important,target
R7931 T9671 T9668 attr target,be
R7932 T9575 T9576 compound brain,injury
R7933 T9672 T9671 prep for,target
R7934 T9673 T9674 amod anti-inflammatory,therapy
R7935 T9674 T9672 pobj therapy,for
R7936 T9576 T9574 pobj injury,after
R7937 T9675 T9668 prep after,be
R7938 T9676 T9675 pobj TBI,after
R7939 T9577 T9566 punct .,indicate
R7940 T9677 T9664 punct .,suggest
R7941 T9578 T9583 cc But,is
R7942 T9579 T9583 prep till,is
R7943 T9580 T9579 pcomp now,till
R7944 T9581 T9583 punct ",",is
R7945 T9582 T9583 expl there,is
R7946 T9583 T9583 ROOT is,is
R7947 T9584 T9585 det no,study
R7948 T9585 T9583 attr study,is
R7949 T9586 T9585 acl focused,study
R7950 T9587 T9586 prep on,focused
R7951 T9588 T9589 det the,relationship
R7952 T9589 T9587 pobj relationship,on
R7953 T9590 T9589 prep among,relationship
R7954 T9591 T9590 pobj astrocytes,among
R7955 T9592 T9589 punct ",",relationship
R7956 T9593 T9589 appos Nrf2,relationship
R7957 T9594 T9593 punct ",",Nrf2
R7958 T9595 T9589 cc and,relationship
R7959 T9596 T9597 amod proinflammatory,mediators
R7960 T9597 T9589 conj mediators,relationship
R7961 T9598 T9586 prep after,focused
R7964 T9599 T9598 pobj TBI,after
R7967 T9600 T9583 punct .,is
R7974 T9601 T9602 poss Our,results
R7985 T9602 T9603 nsubj results,demonstrated
R7989 T9603 T9603 ROOT demonstrated,demonstrated
R7991 T9604 T9606 det the,expression
R7994 T9605 T9606 amod upregulated,expression
R7995 T9606 T9603 dobj expression,demonstrated
R7996 T9607 T9606 prep of,expression
R7998 T9608 T9607 pobj TNF-α,of
R8000 T9609 T9608 punct ",",TNF-α
R8003 T9610 T9608 conj IL-1β,TNF-α
R8004 T9611 T9610 punct ",",IL-1β
R8005 T9612 T9610 conj IL-6,IL-1β
R8006 T9613 T9612 punct ",",IL-6
R8007 T9614 T9612 cc and,IL-6
R8009 T9615 T9612 conj MMP9,IL-6
R8014 T9616 T9615 prep in,MMP9
R8017 T9617 T9618 compound Nrf2,knockout
R8020 T9618 T9616 pobj knockout,in
R8024 T9619 T9606 appos astrocytes,expression
R8031 T9620 T9603 prep after,demonstrated
R8034 T9621 T9622 compound scratch,injury
R8036 T9622 T9620 pobj injury,after
R8038 T9623 T9622 prep for,injury
R8039 T9624 T9626 det the,time
R8043 T9625 T9626 amod first,time
R8047 T9626 T9623 pobj time,for
R8051 T9627 T9603 punct .,demonstrated
R8054 T9628 T9634 prep In,induced
R8055 T9629 T9628 pobj conclusion,In
R8057 T9630 T9634 punct ",",induced
R8058 T9631 T9634 nsubj depletion,induced
R8062 T9632 T9631 prep of,depletion
R8063 T9633 T9632 pobj Nrf2,of
R8064 T9634 T9634 ROOT induced,induced
R8065 T9635 T9636 det the,activation
R8067 T9636 T9634 dobj activation,induced
R8069 T9637 T9636 prep of,activation
R8072 T9638 T9637 pobj NF-κB,of
R8073 T9639 T9636 cc and,activation
R8074 T9640 T9641 det the,expression
R8075 T9641 T9636 conj expression,activation
R8082 T9642 T9641 prep of,expression
R4228 T4973 T4973 ROOT 3.1,3.1
R4229 T4974 T4973 punct .,3.1
R4230 T4975 T4990 nsubjpass Depletion,detected
R4231 T4976 T4975 prep of,Depletion
R4232 T4977 T4978 compound Nrf2,Aggravated
R4233 T4978 T4976 pobj Aggravated,of
R4234 T4979 T4981 det the,Death
R4235 T4980 T4981 compound Cell,Death
R4236 T4981 T4978 dobj Death,Aggravated
R4237 T4982 T4981 prep in,Death
R4238 T4983 T4982 pobj Astrocytes,in
R4239 T4984 T4990 mark after,detected
R4240 T4985 T4988 compound Scratch,death
R4241 T4986 T4987 compound Injury,Cell
R4242 T4987 T4988 compound Cell,death
R4243 T4988 T4990 nsubjpass death,detected
R4244 T4989 T4990 auxpass was,detected
R4245 T4990 T4990 ROOT detected,detected
R4246 T4991 T4990 agent by,detected
R4247 T4992 T4991 pobj microscope,by
R4248 T4993 T4992 cc and,microscope
R4249 T4994 T4995 compound CCK-8,analysis
R4250 T4995 T4992 conj analysis,microscope
R4251 T4996 T4995 prep at,analysis
R4252 T4997 T4998 nummod 24,h
R4253 T4998 T4996 pobj h,at
R4254 T4999 T4990 prep after,detected
R4255 T5000 T4999 pobj scratch,after
R4256 T5001 T4990 punct .,detected
R4257 T5002 T5003 det The,detachment
R4258 T5003 T5009 nsubj detachment,lost
R4259 T5004 T5003 prep from,detachment
R4260 T5005 T5006 compound culture,plate
R4261 T5006 T5004 pobj plate,from
R4262 T5007 T5009 punct ",",lost
R4263 T5008 T5009 nsubj cell,lost
R4264 T5009 T5009 ROOT lost,lost
R4265 T5010 T5009 punct ",",lost
R4266 T5011 T5009 cc and,lost
R1249 T1509 T1510 amod Primary,Culture
R1250 T1510 T1522 nsubjpass Culture,provided
R1251 T1511 T1510 cc and,Culture
R1252 T1512 T1510 conj Identification,Culture
R1254 T1514 T1516 compound Mouse,Nrf2
R1255 T1515 T1516 compound Astrocytes,Nrf2
R1256 T1516 T1518 compound Nrf2,ICR
R1257 T1517 T1518 compound knockout,ICR
R1258 T1518 T1519 compound ICR,mice
R1259 T1519 T1513 pobj mice,of
R1260 T1520 T1522 auxpass were,provided
R1261 T1521 T1522 advmod kindly,provided
R1262 T1522 T1522 ROOT provided,provided
R1263 T1523 T1522 agent by,provided
R1264 T1524 T1527 compound Dr.,Kensler
R1265 T1525 T1527 compound Thomas,Kensler
R1266 T1526 T1527 compound W.,Kensler
R1267 T1527 T1523 pobj Kensler,by
R1268 T1528 T1527 punct (,Kensler
R1269 T1529 T1530 compound Johns,Hopkins
R1270 T1530 T1531 compound Hopkins,University
R1271 T1531 T1527 appos University,Kensler
R1272 T1532 T1531 punct ",",University
R1273 T1533 T1531 conj Baltimore,University
R1274 T1534 T1533 punct ",",Baltimore
R1275 T1535 T1533 conj MD,Baltimore
R1276 T1536 T1535 punct ",",MD
R1277 T1537 T1535 conj USA,MD
R1278 T1538 T1527 punct ),Kensler
R1279 T1539 T1522 punct .,provided
R1280 T1540 T1541 amod Primary,astrocytes
R1281 T1541 T1543 nsubjpass astrocytes,obtained
R1282 T1542 T1543 auxpass were,obtained
R1283 T1543 T1543 ROOT obtained,obtained
R1284 T1544 T1543 prep from,obtained
R1285 T1545 T1549 amod postnatal,type
R1286 T1546 T1549 amod 2-day-old,type
R1300 T1560 T1561 nummod 6,mice
R1301 T1561 T1558 appos mice,mice
R1302 T1562 T1561 prep for,mice
R1303 T1563 T1564 det each,genotype
R1304 T1564 T1562 pobj genotype,for
R1305 T1565 T1558 punct ),mice
R1306 T1566 T1543 punct .,obtained
R1307 T1567 T1573 prep Following,dissected
R1308 T1568 T1567 pobj decapitation,Following
R1309 T1569 T1573 punct ",",dissected
R1377 T1637 T1634 prep with,dissociation
R1378 T1638 T1639 nummod 20,%
R1379 T1639 T1640 compound %,FBS
R1380 T1640 T1637 pobj FBS,with
R1381 T1641 T1640 cc and,FBS
R1382 T1642 T1644 nummod 5,penicillin
R1383 T1643 T1644 amod units/mL,penicillin
R1384 T1644 T1640 conj penicillin,FBS
R1385 T1645 T1644 punct ",",penicillin
R1454 T1714 T1715 nummod 7,days
R1455 T1715 T1712 pobj days,for
R1456 T1716 T1717 aux to,reach
R1457 T1717 T1711 advcl reach,expanded
R1458 T1718 T1717 dobj confluence,reach
R1459 T1719 T1711 punct .,expanded
R1460 T1720 T1726 advmod Then,shaken
R1524 T1784 T1781 pobj studies,in
R1525 T1785 T1775 punct .,passaged
R1623 T1921 T1921 ROOT 2.2,2.2
R1624 T1922 T1921 punct .,2.2
R1625 T1923 T1936 prep In,performed
R1626 T1924 T1925 compound Vitro,Model
R3249 T3755 T3715 prep in,%
R3250 T3756 T3755 pobj PBS,in
R3251 T3757 T3758 quantmod pH,7.4
R3252 T3758 T3715 dep 7.4,%
R3253 T3759 T3758 punct ),7.4
R3254 T3760 T3715 cc and,%
R3255 T3761 T3715 conj centrifuged,%
R3256 T3762 T3761 prep at,centrifuged
R3257 T3763 T3764 nummod "12,000",g
R3258 T3764 T3762 pobj g,at
R3259 T3765 T3761 prep for,centrifuged
R3260 T3766 T3767 nummod 15,min
R3261 T3767 T3765 pobj min,for
R3262 T3768 T3767 prep at,min
R3263 T3769 T3770 nummod 4,°
R3264 T3770 T3773 nummod °,concentrations
R3265 T3771 T3773 nmod C.,concentrations
R3266 T3772 T3773 compound Protein,concentrations
R3267 T3773 T3768 pobj concentrations,at
R3268 T3774 T3775 auxpass were,estimated
R3269 T3775 T3702 conj estimated,obtain
R3270 T3776 T3775 agent by,estimated
R3271 T3777 T3781 compound Coomassie,Reagent
R3272 T3778 T3779 compound Plus,Protein
R3273 T3779 T3781 compound Protein,Reagent
R3274 T3780 T3781 compound Assay,Reagent
R3275 T3781 T3776 pobj Reagent,by
R3276 T3782 T3783 punct (,Pierce
R3277 T3783 T3781 appos Pierce,Reagent
R3278 T3784 T3783 punct ",",Pierce
R3279 T3785 T3783 prep IL,Pierce
R3280 T3786 T3785 punct ",",IL
R3281 T3787 T3785 npadvmod USA,IL
R3282 T3788 T3783 punct ),Pierce
R3283 T3789 T3775 punct .,estimated
R3284 T3790 T3791 nummod Fifty,micrograms
R3285 T3791 T3798 nsubj micrograms,were
R3286 T3792 T3791 prep of,micrograms
R3287 T3793 T3797 det the,extracts
R3288 T3794 T3797 amod resulting,extracts
R3289 T3795 T3797 amod cytosolic,extracts
R3290 T3796 T3797 compound protein,extracts
R3291 T3797 T3792 pobj extracts,of
R3292 T3798 T3798 ROOT were,were
R3293 T3799 T3798 acomp heat-denatured,were
R3294 T3800 T3799 prep in,heat-denatured
R3295 T3801 T3804 compound Laemmli,buffer
R3296 T3802 T3804 compound sample,buffer
R3334 T3840 T3829 prep at,blocked
R3335 T3841 T3842 nummod 4,°
R3336 T3842 T3843 nummod °,C
R3337 T3843 T3840 pobj C,at
R3338 T3844 T3829 punct ",",blocked
R3339 T3845 T3829 cc and,blocked
R3340 T3846 T3848 det the,antibodies
R3341 T3847 T3848 amod following,antibodies
R3342 T3848 T3850 nsubjpass antibodies,used
R3343 T3849 T3850 auxpass were,used
R3344 T3850 T3829 conj used,blocked
R3345 T3851 T3852 punct :,anti-β-actin
R3346 T3852 T3850 prep anti-β-actin,used
R3347 T3853 T3854 punct (,sc-130657
R3348 T3854 T3852 dep sc-130657,anti-β-actin
R3349 T3855 T3854 punct ",",sc-130657
R3350 T3856 T3858 compound Santa,Biotechnology
R3351 T3857 T3858 compound Cruz,Biotechnology
R3352 T3858 T3854 conj Biotechnology,sc-130657
R3353 T3859 T3858 punct ",",Biotechnology
R3354 T3860 T3858 conj CA,Biotechnology
R3411 T3917 T3914 prep in,washed
R3412 T3918 T3920 det the,buffer
R3413 T3919 T3920 amod washing,buffer
R3414 T3920 T3917 pobj buffer,in
R3415 T3921 T3914 cc and,washed
R3416 T3922 T3914 conj covered,washed
R3417 T3923 T3922 prep with,covered
R3418 T3924 T3928 det the,antibody
R3419 T3925 T3928 amod horseradish,antibody
R3420 T3926 T3928 amod peroxidase-linked,antibody
R3421 T3927 T3928 amod secondary,antibody
R3422 T3928 T3923 pobj antibody,with
R3423 T3929 T3922 prep at,covered
R3424 T3930 T3931 det a,1
R3425 T3931 T3929 pobj 1,at
R3426 T3932 T3934 punct :,dilution
R3427 T3933 T3934 nummod 2000,dilution
R3428 T3934 T3940 nsubjpass dilution,incubated
R3429 T3935 T3934 prep for,dilution
R3430 T3936 T3938 nummod 1,Blots
R3431 T3937 T3938 compound h.,Blots
R3432 T3938 T3935 pobj Blots,for
R3876 T4563 T4564 compound Zymography,Cells
R3877 T4564 T4569 nsubjpass Cells,homogenized
R3878 T4565 T4564 prep of,Cells
R3879 T4566 T4567 nummod four,groups
R3880 T4567 T4565 pobj groups,of
R3881 T4568 T4569 auxpass were,homogenized
R3882 T4569 T4569 ROOT homogenized,homogenized
R3883 T4570 T4569 prep in,homogenized
R3884 T4571 T4572 compound lysis,buffer
R3885 T4572 T4570 pobj buffer,in
R3886 T4573 T4572 acl containing,buffer
R3964 T4651 T4652 nummod 2.5,%
R3965 T4652 T4654 compound %,X-100
R3966 T4653 T4654 compound Triton,X-100
R3967 T4654 T4650 pobj X-100,in
R3968 T4655 T4647 cc and,renatured
R3969 T4656 T4657 advmod then,incubated
R3970 T4657 T4647 conj incubated,renatured
R3971 T4658 T4657 prep in,incubated
R3972 T4659 T4660 compound substrate,buffer
R3973 T4660 T4658 pobj buffer,in
R3974 T4661 T4660 prep for,buffer
R4032 T4719 T4721 compound MMP9,density
R4033 T4720 T4721 compound band,density
R4034 T4721 T4718 pobj density,of
R4035 T4722 T4723 auxpass was,performed
R4036 T4723 T4723 ROOT performed,performed
R4037 T4724 T4723 prep with,performed
R4038 T4725 T4726 compound image,analysis
R4039 T4726 T4727 compound analysis,program
R4040 T4727 T4728 compound program,ImageJ
R4041 T4728 T4724 pobj ImageJ,with
R4042 T4729 T4723 punct .,performed
R7071 T8743 T8745 det The,study
R7072 T8744 T8745 amod present,study
R7073 T8745 T8746 nsubj study,demonstrated
R7074 T8746 T8746 ROOT demonstrated,demonstrated
R7075 T8747 T8750 mark that,induced
R7076 T8748 T8749 compound scratch,injury
R7077 T8749 T8750 nsubj injury,induced
R7078 T8750 T8746 ccomp induced,demonstrated
R7079 T8751 T8752 det the,upregulation
R7080 T8752 T8750 dobj upregulation,induced
R7081 T8753 T8752 prep of,upregulation
R7082 T8754 T8755 compound NF-κB,DNA
R7083 T8755 T8753 pobj DNA,of
R7084 T8756 T8757 amod binding,activity
R7085 T8757 T8753 pobj activity,of
R7086 T8758 T8757 cc and,activity
R7087 T8759 T8757 conj overexpression,activity
R7088 T8760 T8759 prep of,overexpression
R7089 T8761 T8760 pobj TNF-α,of
R7090 T8762 T8761 punct ",",TNF-α
R7151 T8823 T8821 punct ",",[
R7152 T8824 T8825 nummod 9,]
R7153 T8825 T8821 appos ],[
R7154 T8826 T8808 punct .,demonstrated
R7155 T8827 T8839 prep As,activated
R7156 T8828 T8830 det a,factor
R7157 T8829 T8830 compound transcript,factor
R7158 T8830 T8827 pobj factor,As
R7159 T8831 T8839 punct ",",activated
R7160 T8832 T8833 amod NF-κB,binds
R7161 T8833 T8839 nsubjpass binds,activated
R7162 T8834 T8833 prep with,binds
R7163 T8835 T8834 pobj DNA,with
R7164 T8836 T8839 mark once,activated
R7165 T8837 T8839 nsubjpass it,activated
R7166 T8838 T8839 auxpass was,activated
R7229 T8901 T8904 nsubjpass It,demonstrated
R7230 T8902 T8904 aux has,demonstrated
R7231 T8903 T8904 auxpass been,demonstrated
R7232 T8904 T8904 ROOT demonstrated,demonstrated
R7233 T8905 T8907 mark that,is
R7234 T8906 T8907 expl there,is
R7235 T8907 T8904 ccomp is,demonstrated
R7236 T8908 T8910 det an,loop
R7237 T8909 T8910 amod autoregulatory,loop
R7238 T8910 T8907 attr loop,is
R7239 T8911 T8910 prep among,loop
R7240 T8912 T8914 det these,mediators
R7241 T8913 T8914 amod proinflammatory,mediators
R7242 T8914 T8911 pobj mediators,among
R7243 T8915 T8904 punct .,demonstrated
R7244 T8916 T8922 prep For,are
R7245 T8917 T8916 pobj example,For
R7246 T8918 T8922 punct ",",are
R7247 T8919 T8922 nsubj TNF-α,are
R7308 T8980 T8977 prep to,induces
R7309 T8981 T8980 pobj brain,to
R7310 T8982 T8970 punct .,aggravates
R7311 T8983 T8984 nsubj It,is
R7312 T8984 T8984 ROOT is,is
R7313 T8985 T8987 det a,choice
R7314 T8986 T8987 amod good,choice
R7315 T8987 T8984 attr choice,is
R7316 T8988 T8987 prep for,choice
R7317 T8989 T8988 pcomp targeting,for
R7318 T8990 T8989 prep on,targeting
R7319 T8991 T8993 det an,factor
R7320 T8992 T8993 amod upstream,factor
R7321 T8993 T8990 pobj factor,on
R7322 T8994 T8995 aux to,prevent
R7323 T8995 T8993 acl prevent,factor
R7324 T8996 T8998 amod such,loop
R7325 T8997 T8998 amod autoregulatory,loop
R7326 T9025 T9024 punct ",",metabolites
R7372 T9057 T9056 prep of,transcription
R7373 T9058 T9059 det a,group
R7379 T9059 T9057 pobj group,of
R7380 T9060 T9059 prep of,group
R7382 T9061 T9062 amod antioxidant,genes
R7383 T9062 T9060 pobj genes,of
R7384 T9063 T9062 punct ",",genes
R7386 T9064 T9065 amod such,as
R7387 T9065 T9062 prep as,genes
R7389 T9066 T9067 compound heme,oxygenase-1
R7392 T9067 T9065 pobj oxygenase-1,as
R7393 T9068 T9069 punct (,HO-1
R7395 T9069 T9067 appos HO-1,oxygenase-1
R7396 T9070 T9069 punct ),HO-1
R7397 T9071 T9067 cc and,oxygenase-1
R7399 T9072 T9067 conj NAD,oxygenase-1
R7400 T9073 T9072 punct (,NAD
R7402 T9074 T9072 appos P,NAD
R7403 T9075 T9072 punct ),NAD
R7405 T9076 T9065 pobj H,as
R7406 T9077 T9055 punct :,activates
R7484 T9135 T9134 prep of,subfield
R7486 T9136 T9135 pobj hippocampus,of
R7487 T9137 T9113 prep after,decreases
R7489 T9138 T9139 compound TBI,[
R7490 T9139 T9141 nmod [,]
R7492 T9140 T9141 nummod 19,]
R7493 T9141 T9137 pobj ],after
R7494 T9142 T9107 punct .,improves
R7496 T9143 T9150 prep On,appear
R7497 T9144 T9146 det the,hand
R7404 T9156 T9159 nsubj Depletion,induces
R7408 T9157 T9156 prep of,Depletion
R7412 T9158 T9157 pobj Nrf2,of
R7416 T9159 T9159 ROOT induces,induces
R7419 T9160 T9161 amod higher,expression
R7425 T9161 T9159 dobj expression,induces
R7431 T9162 T9161 prep of,expression
R7435 T9163 T9164 amod proinflammatory,mediators
R7437 T9164 T9162 pobj mediators,of
R7440 T9165 T9159 prep in,induces
R7592 T9214 T9211 pobj mediators,of
R7594 T9215 T9194 conj led,aggravated
R7598 T9216 T9215 prep to,led
R7602 T9217 T9219 amod more,deaths
R7604 T9218 T9219 compound astrocytes,deaths
R7608 T9219 T9216 pobj deaths,to
R7611 T9220 T9177 punct .,revealed
R7615 T9221 T9222 nsubj Data,obtained
R7619 T9222 T9222 ROOT obtained,obtained
R7623 T9223 T9222 prep from,obtained
R7704 T9381 T9381 ROOT ],]
R7705 T9382 T9363 punct .,discovered
R7707 T9383 T9387 advmod Interestingly,reported
R7708 T9384 T9387 punct ",",reported
R7709 T9385 T9387 nsubjpass it,reported
R7711 T9386 T9387 auxpass is,reported
R7712 T9387 T9387 ROOT reported,reported
R7713 T9388 T9391 mark that,inhibit
R7715 T9389 T9391 nsubj NF-κB,inhibit
R7751 T9451 T9453 nmod [,]
R7752 T9452 T9453 nummod 26,]
R7754 T9453 T9450 dobj ],pathway
R7755 T9454 T9427 punct .,indicate
R7757 T9455 T9458 nsubjpass It,confirmed
R7758 T9456 T9458 aux has,confirmed
R7760 T9457 T9458 auxpass been,confirmed
R7761 T9458 T9458 ROOT confirmed,confirmed
R7762 T9459 T9463 mark that,detected
R7764 T9460 T9463 nsubjpass Nrf2,detected
R7767 T9461 T9463 auxpass is,detected
R7768 T9462 T9463 advmod mainly,detected
R7770 T9463 T9458 ccomp detected,confirmed
R7771 T9464 T9463 prep in,detected
R7773 T9465 T9464 pobj nucleus,in
R7774 T9466 T9465 prep of,nucleus
R7775 T9467 T9466 pobj astrocytes,of
R7776 T9468 T9463 prep after,detected
R7777 T9469 T9470 compound TBI,[
R7779 T9470 T9468 pobj [,after
R7862 T9530 T9529 pobj treatment,after
R7863 T9531 T9530 prep with,treatment
R7865 T9532 T9531 pobj LPS,with
R7866 T9533 T9530 cc or,treatment
R7868 T9534 T9535 compound oxyhemoglobin,[
R7869 T9535 T9516 dobj [,identified
R7870 T9536 T9535 nummod 27,[
R7872 T9537 T9535 punct ",",[
R7873 T9538 T9539 nummod 28,]
R4966 T5946 T5946 ROOT 3.3,3.3
R4967 T5947 T5946 punct .,3.3
R4968 T5948 T5952 nsubj Expression,Was
R4969 T5949 T5948 prep of,Expression
R4970 T5950 T5951 compound Proinflammatory,Cytokines
R4971 T5951 T5949 pobj Cytokines,of
R4972 T5952 T5952 ROOT Was,Was
R4973 T5953 T5952 attr Elevated,Was
R4974 T5954 T5953 prep in,Elevated
R4975 T5955 T5956 compound Nrf2,Knockout
R4976 T5956 T5954 pobj Knockout,in
R4977 T5957 T5953 conj Astrocytes,Elevated
R4978 T5958 T5952 prep after,Was
R4979 T5959 T5960 compound Scratch,Injury
R4980 T5960 T5958 pobj Injury,after
R4981 T5961 T5964 mark As,know
R4982 T5962 T5964 nsubj we,know
R4983 T5963 T5962 appos all,we
R4984 T5964 T5973 advcl know,regulated
R5064 T6044 T6038 prep in,relationship
R5065 T6045 T6044 pobj astrocytes,in
R5066 T6046 T6038 prep after,relationship
R5067 T6047 T6046 pobj TBI,after
R5068 T6048 T6033 punct .,is
R5069 T6049 T6052 advmod Here,tested
R5070 T6050 T6052 punct ",",tested
R5071 T6051 T6052 nsubj we,tested
R5072 T6052 T6052 ROOT tested,tested
R5073 T6053 T6054 det the,levels
R5074 T6054 T6052 dobj levels,tested
R5075 T6055 T6054 prep of,levels
R5076 T6056 T6055 pobj TNF-α,of
R5077 T6057 T6056 punct ",",TNF-α
R5078 T6058 T6056 conj IL-1β,TNF-α
R5079 T6059 T6058 punct ",",IL-1β
R5080 T6060 T6058 cc and,IL-1β
R5081 T6061 T6058 conj IL-6,IL-1β
R5082 T6062 T6054 prep in,levels
R5083 T6063 T6064 amod cultured,astrocytes
R5084 T6064 T6062 pobj astrocytes,in
R5085 T6065 T6052 prep at,tested
R5086 T6066 T6067 nummod 24,h
R5087 T6067 T6065 pobj h,at
R5088 T6068 T6052 prep after,tested
R5089 T6069 T6070 compound scratch,injury
R5090 T6070 T6068 pobj injury,after
R5091 T6071 T6070 prep by,injury
R5092 T6072 T6071 pobj RT-PCR,by
R5093 T6073 T6072 cc and,RT-PCR
R5094 T6074 T6072 conj ELISA,RT-PCR
R5095 T6075 T6052 punct .,tested
R5096 T6076 T6078 nsubjpass It,shown
R5097 T6077 T6078 auxpass was,shown
R5098 T6078 T6078 ROOT shown,shown
R5099 T6079 T6091 mark that,increased
R5100 T6080 T6082 det the,levels
R5101 T6081 T6082 amod mRNA,levels
R5102 T6082 T6091 nsubjpass levels,increased
R5103 T6083 T6082 prep of,levels
R5104 T6084 T6083 pobj TNF-α,of
R5105 T6085 T6084 punct ",",TNF-α
R5106 T6086 T6084 conj IL-1β,TNF-α
R5107 T6087 T6086 punct ",",IL-1β
R5108 T6088 T6086 cc and,IL-1β
R5109 T6089 T6086 conj IL-6,IL-1β
R5110 T6090 T6091 auxpass were,increased
R5111 T6091 T6078 ccomp increased,shown
R5112 T6092 T6091 prep after,increased
R5113 T6093 T6092 pobj scratch,after
R2354 T2715 T2713 punct ),pH
R5272 T6252 T6244 punct .,observed
R5273 T6253 T6257 prep For,was
R5274 T6254 T6253 pobj IL-6,For
R5275 T6255 T6257 punct ",",was
R5276 T6256 T6257 nsubj it,was
R5277 T6257 T6257 ROOT was,was
R5278 T6258 T6260 nummod 36.07,0.786
R5279 T6259 T6260 nummod ±,0.786
R5280 T6260 T6257 attr 0.786,was
R5281 T6261 T6260 prep for,0.786
R5282 T6262 T6264 compound group,TBI
R5283 T6263 T6264 compound KO,TBI
R5284 T6264 T6261 pobj TBI,for
R5285 T6265 T6257 prep while,was
R5336 T6316 T6315 punct ),c
R5337 T6317 T6301 punct ),5.348
R5338 T6318 T6290 punct .,was
R709 T917 T918 compound Brain,damage
R710 T918 T922 nsubj damage,is
R711 T919 T918 acl following,damage
R712 T920 T921 amod traumatic,injury
R713 T921 T919 dobj injury,following
R2355 T2716 T2711 punct ",",HEPES
R2356 T2717 T2719 nummod 10,KCl
R2357 T2718 T2719 compound mM,KCl
R2358 T2719 T2711 conj KCl,HEPES
R2426 T2787 T2788 compound mM,DTT
R2427 T2788 T2784 conj DTT,EGTA
R2428 T2789 T2788 punct ",",DTT
R2429 T2790 T2788 cc and,DTT
R2430 T2791 T2794 nummod 1.0,fluoride
R2431 T2792 T2794 compound mM,fluoride
R2432 T2793 T2794 compound phenylmethylsulfonyl,fluoride
R2433 T2794 T2788 conj fluoride,DTT
R2434 T2795 T2762 punct .,extracted
R2435 T2796 T2797 amod Insoluble,material
R2436 T2797 T2799 nsubjpass material,removed
R2437 T2798 T2799 auxpass was,removed
R2438 T2799 T2799 ROOT removed,removed
R2439 T2800 T2799 agent by,removed
R2440 T2801 T2800 pobj centrifugation,by
R2441 T2802 T2799 prep at,removed
R2442 T2803 T2804 nummod 14000,rpm
R2443 T2804 T2802 pobj rpm,at
R2444 T2805 T2799 punct ",",removed
R2445 T2806 T2799 cc and,removed
R2446 T2807 T2808 det the,supernatant
R2447 T2808 T2814 nsubjpass supernatant,stored
R2448 T2809 T2808 acl containing,supernatant
R2449 T2810 T2812 det the,proteins
R2450 T2811 T2812 amod nuclear,proteins
R2451 T2812 T2809 dobj proteins,containing
R2504 T2865 T2863 conj USA,WI
R2505 T2866 T2857 punct ),system
R2506 T2867 T2852 punct .,performed
R2507 T2868 T2870 compound Consensus,probe
R2508 T2869 T2870 compound oligonucleotide,probe
R2509 T2870 T2879 nsubjpass probe,end-labeled
R2510 T2871 T2870 punct (,probe
R2511 T2872 T2875 nummod 5,AGTTGAGGGGACTTTCCCAGGG-3
R2512 T2873 T2875 punct ′,AGTTGAGGGGACTTTCCCAGGG-3
R2513 T2874 T2875 punct -,AGTTGAGGGGACTTTCCCAGGG-3
R2514 T2875 T2870 appos AGTTGAGGGGACTTTCCCAGGG-3,probe
R2515 T2876 T2875 appos ′,AGTTGAGGGGACTTTCCCAGGG-3
R2516 T2877 T2875 punct ),AGTTGAGGGGACTTTCCCAGGG-3
R2517 T2878 T2879 auxpass was,end-labeled
R2595 T2956 T2959 nmod μL,probe
R2596 T2957 T2959 amod 32P-labled,probe
R2597 T2958 T2959 compound oligonucleotide,probe
R2598 T2959 T2953 pobj probe,of
R2599 T2960 T2964 punct ",",continued
R2600 T2961 T2962 det the,incubation
R2601 T2962 T2964 nsubjpass incubation,continued
R2602 T2963 T2964 auxpass was,continued
R2603 T2964 T2964 ROOT continued,continued
R2604 T2965 T2964 prep for,continued
R2605 T2966 T2967 nummod 20,minutes
R2606 T2967 T2965 pobj minutes,for
R2607 T2968 T2967 prep on,minutes
R2608 T2969 T2968 pobj ice,on
R2609 T2970 T2964 punct .,continued
R2610 T2971 T2973 det The,complexes
R2639 T3000 T2998 pobj °,at
R2640 T3001 T2975 npadvmod C,separated
R2641 T3002 T2975 punct .,separated
R2642 T3003 T3009 prep After,dried
R2643 T3004 T3003 pobj electrophoresis,After
R2644 T3005 T3009 punct ",",dried
R2645 T3006 T3007 det the,gel
R2646 T3007 T3009 nsubjpass gel,dried
R2647 T3008 T3009 auxpass was,dried
R2648 T3009 T3009 ROOT dried,dried
R2649 T3010 T3009 cc and,dried
R2650 T3011 T3009 conj exposed,dried
R2651 T3012 T3011 prep to,exposed
R2652 T3013 T3014 compound X-ray,film
R2653 T3014 T3012 pobj film,to
R2654 T3015 T3017 punct (,Hyperfilm
R2655 T3016 T3017 compound Fuji,Hyperfilm
R2656 T3017 T3014 appos Hyperfilm,film
R2657 T3018 T3017 punct ",",Hyperfilm
R2658 T3019 T3017 npadvmod Tokyo,Hyperfilm
R2659 T3020 T3019 punct ",",Tokyo
R2660 T3021 T3019 conj Japan,Tokyo
R2661 T3022 T3017 punct ),Hyperfilm
R2662 T3023 T3009 punct .,dried
R2663 T3024 T3031 nsubjpass Levels,quantified
R217 T315 T316 det The,Absence
R218 T316 T333 nsubj Absence,proved
R219 T317 T316 prep of,Absence
R220 T318 T319 compound Nrf2,Enhances
R221 T319 T317 pobj Enhances,of
R222 T320 T321 compound NF-κB-Dependent,Inflammation
R223 T321 T316 appos Inflammation,Absence
R224 T322 T333 prep following,proved
R225 T323 T324 compound Scratch,Injury
R226 T324 T322 dobj Injury,following
R227 T325 T324 prep in,Injury
R228 T326 T329 compound Mouse,Astrocytes
R229 T327 T328 compound Primary,Cultured
R230 T328 T329 compound Cultured,Astrocytes
R231 T329 T325 pobj Astrocytes,in
R232 T330 T333 nsubjpass It,proved
R233 T331 T333 aux has,proved
R234 T332 T333 auxpass been,proved
R235 T333 T333 ROOT proved,proved
R236 T334 T337 mark that,enhances
R237 T335 T336 amod Nrf2,depletion
R238 T336 T337 nsubj depletion,enhances
R239 T337 T333 ccomp enhances,proved
R240 T338 T339 amod inflammatory,process
R241 T339 T337 dobj process,enhances
R242 T340 T337 prep through,enhances
R243 T341 T340 pobj activation,through
R244 T342 T341 prep of,activation
R245 T343 T342 pobj NF-κB,of
R246 T344 T341 prep in,activation
R336 T434 T433 pobj TNF-α,of
R337 T435 T434 punct ",",TNF-α
R338 T436 T434 conj IL-1β,TNF-α
R339 T437 T436 punct ",",IL-1β
R340 T438 T436 conj IL-6,IL-1β
R341 T439 T438 punct ",",IL-6
R342 T440 T438 cc and,IL-6
R343 T441 T438 conj MMP9,IL-6
R344 T442 T444 auxpass were,evaluated
R345 T443 T444 advmod also,evaluated
R346 T444 T444 ROOT evaluated,evaluated
R347 T445 T444 punct .,evaluated
R348 T446 T447 compound Gelatin,zymography
R402 T500 T501 compound WT,astrocytes
R403 T501 T499 pobj astrocytes,in
R404 T502 T489 punct .,was
R405 T503 T504 det These,results
R406 T504 T505 nsubj results,suggest
R407 T505 T505 ROOT suggest,suggest
R408 T506 T512 mark that,induce
R409 T507 T508 det the,absence
R410 T508 T512 nsubj absence,induce
R411 T509 T508 prep of,absence
R412 T510 T509 pobj Nrf2,of
R413 T511 T512 aux may,induce
R414 T512 T505 ccomp induce,suggest
R415 T513 T514 advmod more,aggressive
R416 T514 T515 amod aggressive,inflammation
R417 T515 T512 dobj inflammation,induce
R418 T516 T512 prep through,induce
R419 T517 T516 pobj activation,through
R420 T518 T517 prep of,activation
R421 T519 T518 pobj NF-κB,of
R422 T520 T517 cc and,activation
R423 T521 T523 amod downstream,cytokines
R424 T522 T523 compound proinflammatory,cytokines
R425 T523 T517 conj cytokines,activation
R426 T524 T512 prep in,induce
R427 T525 T524 pobj astrocytes,in
R428 T526 T505 punct .,suggest
R5596 T6759 T6759 ROOT 3.4,3.4
R5597 T6760 T6759 punct .,3.4
R5598 T6761 T6768 nsubjpass Expression,Enhanced
R5599 T6762 T6761 cc and,Expression
R5600 T6763 T6761 conj Activity,Expression
R5601 T6764 T6763 prep of,Activity
R5694 T6857 T6859 compound group,TBI
R5695 T6858 T6859 compound WT,TBI
R5696 T6859 T6856 pobj TBI,in
R5697 T6860 T6859 punct (,TBI
R5698 T6861 T6862 nummod 1.36,±
R5699 T6862 T6870 nummod ±,<
R5700 T6863 T6870 dep 0.090,<
R5701 T6864 T6870 prep versus,<
R5702 T6865 T6866 nummod 1.01,±
R5703 T6866 T6867 nummod ±,0.068
R5704 T6867 T6864 pobj 0.068,versus
R5705 T6868 T6870 punct ",",<
R5706 T6869 T6870 nsubj P,<
R5707 T6870 T6859 appos <,TBI
R5708 T6871 T6870 npadvmod 0.01,<
R5709 T6872 T6870 punct ),<
R5710 T6873 T6859 punct (,TBI
R5711 T6874 T6873 dep Figures,(
R5712 T6875 T6874 nummod 3,Figures
R5713 T6876 T6877 punct (,a
R5714 T6877 T6875 parataxis a,3
R5715 T6878 T6877 punct ),a
R5716 T6879 T6875 cc and,3
R5717 T6880 T6875 conj 3,3
R5718 T6881 T6882 punct (,e
R5719 T6882 T6874 parataxis e,Figures
R5720 T6883 T6882 punct ),e
R5721 T6884 T6882 punct ),e
R5722 T6885 T6874 punct .,Figures
R5723 T6886 T6887 amod Significant,difference
R5724 T6887 T6890 nsubjpass difference,discovered
R5725 T6888 T6890 auxpass was,discovered
R5726 T6889 T6890 advmod also,discovered
R5727 T6890 T6890 ROOT discovered,discovered
R5728 T6891 T6890 prep in,discovered
R5729 T6892 T6894 nummod MMP9,level
R5730 T6893 T6894 compound protein,level
R5731 T6894 T6891 pobj level,in
R5732 T6895 T6890 agent by,discovered
R5733 T6896 T6897 amod western,blot
R5734 T6897 T6895 pobj blot,by
R5735 T6898 T6890 punct ",",discovered
R5736 T6899 T6890 prep as,discovered
R5737 T6900 T6901 nummod 1.44,±
R5738 T6901 T6902 compound ±,0.076
R5739 T6902 T6899 pobj 0.076,as
R5740 T6903 T6902 prep for,0.076
R5741 T6904 T6906 compound group,TBI
R5742 T6905 T6906 compound KO,TBI
R5743 T6906 T6903 pobj TBI,for
R5744 T6907 T6902 cc and,0.076
R5745 T6908 T6909 nummod 1.13,±
R5746 T6909 T6902 conj ±,0.076
R5747 T6910 T6909 conj 0.048,±
R5748 T6911 T6910 prep for,0.048
R5749 T6912 T6911 pobj group,for
R5750 T6913 T6914 compound WT,TBI
R5751 T6914 T6912 appos TBI,group
R5752 T6915 T6916 punct (,P
R5753 T6916 T6914 appos P,TBI
R5754 T6917 T6918 compound <,0.01
R5755 T6918 T6916 nummod 0.01,P
R5756 T6919 T6914 punct ),TBI
R5757 T6920 T6921 punct (,Figure
R5758 T6921 T6912 appos Figure,group
R5759 T6922 T6921 nummod 5,Figure
R5760 T6923 T6921 punct ),Figure
R4445 T5227 T5227 ROOT 3.2,3.2
R4446 T5228 T5227 punct .,3.2
R4447 T5229 T5244 nsubj Disruption,reported
R4448 T5230 T5229 prep of,Disruption
R4449 T5231 T5233 compound Nrf2,Upregulation
R4450 T5232 T5233 compound Enhanced,Upregulation
R4451 T5233 T5230 pobj Upregulation,of
R5772 T6935 T6930 prep than,activity
R5773 T6936 T6935 pobj that,than
R5774 T6937 T6936 prep in,that
R5775 T6938 T6940 compound group,TBI
R5776 T6939 T6940 compound WT,TBI
R5777 T6940 T6937 pobj TBI,in
R5778 T6941 T6927 advmod too,revealed
R5779 T6942 T6927 punct (,revealed
R5780 T6943 T6944 nummod 101.76,±
R5781 T6944 T6945 nummod ±,6.343
R5782 T6945 T6927 npadvmod 6.343,revealed
R5783 T6946 T6945 prep versus,6.343
R5784 T6947 T6949 nummod 76.32,3.388
R5785 T6948 T6949 nummod ±,3.388
R5786 T6949 T6946 pobj 3.388,versus
R5787 T6950 T6952 punct ",",<
R5788 T6951 T6952 nsubj P,<
R5789 T6952 T6952 ROOT <,<
R5790 T6953 T6952 npadvmod 0.01,<
R5791 T6954 T6952 punct ),<
R5792 T6955 T6956 punct (,Figure
R5793 T6956 T6952 appos Figure,<
R5794 T6957 T6956 nummod 6,Figure
R5795 T6958 T6956 punct ),Figure
R5796 T6959 T6952 punct .,<
R1915 T2242 T2241 prep into,added

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T287 15-34 Protein denotes Nrf2 Enhances NF-κB
T8742 20667-20671 Protein denotes Nrf2
T8741 20503-20513 Positive_regulation denotes activation
T8740 20503-20513 Positive_regulation denotes activation
T8739 20503-20513 Positive_regulation denotes activation
T8738 20503-20513 Positive_regulation denotes activation
T8737 20491-20498 Positive_regulation denotes induced
T8736 20491-20498 Positive_regulation denotes induced
T8735 20491-20498 Positive_regulation denotes induced
T8734 20491-20498 Positive_regulation denotes induced
T8733 20491-20498 Positive_regulation denotes induced
T8732 20574-20583 Gene_expression denotes resulting
T8731 20574-20583 Gene_expression denotes resulting
T8730 20574-20583 Gene_expression denotes resulting
T8729 20574-20583 Gene_expression denotes resulting
T8728 20531-20541 Gene_expression denotes expression
T8727 20531-20541 Gene_expression denotes expression
T8726 20531-20541 Gene_expression denotes expression
T8725 20531-20541 Gene_expression denotes expression
T8694 19856-19860 Protein denotes IL-6
T8693 19845-19850 Protein denotes IL-1β
T8692 19838-19843 Protein denotes TNF-α
T8691 19752-19762 Positive_regulation denotes activation
T8690 19752-19762 Positive_regulation denotes activation
T8689 19771-19774 Protein denotes ARE
T8688 19766-19770 Protein denotes Nrf2
T8687 19601-19605 Protein denotes Nrf2
T8686 19526-19534 Gene_expression denotes detected
T8685 19511-19515 Protein denotes Nrf2
T8684 19403-19412 Regulation denotes regulated
T8683 19403-19412 Regulation denotes regulated
T8682 19463-19468 Protein denotes MAPKs
T8681 19429-19461 Protein denotes mitogen-activated protein kinase
T8680 19384-19388 Protein denotes Nrf2
T8679 19374-19379 Protein denotes NF-κB
T8678 19221-19232 Binding denotes interaction
T8677 19273-19280 Protein denotes histone
T8676 19260-19263 Protein denotes CBP
T8675 19242-19256 Protein denotes CH1-KIX domain
T8674 19169-19172 Protein denotes ARE
T8673 19164-19168 Protein denotes Nrf2
T8672 19132-19149 Protein denotes NF-κB p65 subunit
T8671 19093-19100 Negative_regulation denotes inhibit
T8670 19101-19105 Protein denotes Nrf2
T8669 19083-19088 Protein denotes NF-κB
T8668 18900-18910 Positive_regulation denotes activation
T8667 18891-18899 Positive_regulation denotes Enhanced
T8666 19001-19010 Negative_regulation denotes deficient
T8665 18900-18910 Positive_regulation denotes activation
T8664 18996-19000 Protein denotes Nrf2
T8663 18914-18919 Protein denotes NF-κB
T8662 18661-18668 Positive_regulation denotes induces
T8661 18669-18681 Positive_regulation denotes augmentation
T8660 18643-18652 Negative_regulation denotes depletion
T8659 18685-18690 Protein denotes NF-κB
T8658 18656-18660 Protein denotes Nrf2
T8657 18566-18569 Protein denotes SFN
T8656 18541-18545 Protein denotes Nrf2
T8655 18457-18462 Protein denotes NF-κB
T8654 18329-18336 Negative_regulation denotes inhibit
T8653 18329-18336 Negative_regulation denotes inhibit
T8652 18329-18336 Negative_regulation denotes inhibit
T8651 18329-18336 Negative_regulation denotes inhibit
T8650 18329-18336 Negative_regulation denotes inhibit
T8649 18391-18404 Localization denotes translocation
T8648 18391-18404 Localization denotes translocation
T8647 18391-18404 Localization denotes translocation
T8646 18383-18390 Entity denotes nuclear
T8645 18345-18360 Phosphorylation denotes phosphorylation
T8644 18345-18360 Phosphorylation denotes phosphorylation
T8643 18431-18436 Protein denotes NF-κB
T8642 18372-18374 Protein denotes κB
T8641 18365-18371 Protein denotes p65 NF
T8640 18341-18344 Protein denotes IκB
T8639 18337-18340 Protein denotes IKK
T8638 18302-18305 Protein denotes SFN
T8607 17263-17267 Protein denotes HO-1
T8606 17309-17313 Protein denotes NQO1
T8605 17245-17261 Protein denotes heme oxygenase-1
T8604 17175-17179 Protein denotes Nrf2
T8603 17104-17116 Positive_regulation denotes augmentation
T8602 17120-17124 Protein denotes Nrf2
T8601 16939-16948 Regulation denotes regulator
T8600 16897-16900 Protein denotes ARE
T8599 16892-16896 Protein denotes Nrf2
T8598 16631-16637 Positive_regulation denotes induce
T8597 16631-16637 Positive_regulation denotes induce
T8596 16534-16541 Positive_regulation denotes induces
T8595 16638-16648 Positive_regulation denotes activation
T8594 16542-16556 Positive_regulation denotes overexpression
T8593 16480-16491 Positive_regulation denotes stimulators
T8592 16480-16491 Positive_regulation denotes stimulators
T8591 16652-16657 Protein denotes NF-κB
T8590 16616-16621 Protein denotes IL-1β
T8589 16606-16611 Protein denotes TNF-α
T8588 16560-16564 Protein denotes MMP9
T8587 16529-16533 Protein denotes IL-6
T8586 16496-16500 Protein denotes MMP9
T8585 16463-16468 Protein denotes IL-1β
T8584 16453-16458 Protein denotes TNF-α
T8583 16204-16213 Positive_regulation denotes elevation
T8582 16196-16203 Positive_regulation denotes induced
T8581 16196-16203 Positive_regulation denotes induced
T8580 16196-16203 Positive_regulation denotes induced
T8579 16245-16259 Positive_regulation denotes overexpression
T8578 16245-16259 Gene_expression denotes overexpression
T8577 16204-16213 Positive_regulation denotes elevation
T8576 16263-16267 Protein denotes MMP9
T8575 16217-16222 Protein denotes NF-κB
T8574 16002-16009 Positive_regulation denotes induces
T8573 16010-16023 Transcription denotes transcription
T8572 16104-16108 Protein denotes IL-6
T8571 16093-16098 Protein denotes IL-1β
T8570 16086-16091 Protein denotes TNF-α
T8569 16027-16031 Protein denotes MMP9
T8568 15955-15960 Protein denotes NF-κB
T8567 15865-15874 Positive_regulation denotes activated
T8566 15856-15861 Protein denotes NF-κB
T8565 15649-15656 Positive_regulation denotes induced
T8564 15695-15705 Gene_expression denotes expression
T8563 15738-15742 Protein denotes Nrf2
T8562 15676-15681 Protein denotes NF-κB
T8561 15482-15489 Positive_regulation denotes induced
T8560 15482-15489 Positive_regulation denotes induced
T8559 15482-15489 Positive_regulation denotes induced
T8558 15482-15489 Positive_regulation denotes induced
T8557 15494-15506 Positive_regulation denotes upregulation
T8556 15494-15506 Positive_regulation denotes upregulation
T8555 15494-15506 Positive_regulation denotes upregulation
T8554 15494-15506 Positive_regulation denotes upregulation
T8553 15482-15489 Positive_regulation denotes induced
T8552 15482-15489 Positive_regulation denotes induced
T8551 15482-15489 Positive_regulation denotes induced
T8550 15482-15489 Positive_regulation denotes induced
T8549 15482-15489 Positive_regulation denotes induced
T8548 15482-15489 Positive_regulation denotes induced
T8547 15482-15489 Positive_regulation denotes induced
T8546 15482-15489 Positive_regulation denotes induced
T8545 15541-15555 Positive_regulation denotes overexpression
T8544 15541-15555 Positive_regulation denotes overexpression
T8543 15541-15555 Positive_regulation denotes overexpression
T8542 15541-15555 Positive_regulation denotes overexpression
T8541 15541-15555 Gene_expression denotes overexpression
T8540 15541-15555 Gene_expression denotes overexpression
T8539 15541-15555 Gene_expression denotes overexpression
T8538 15541-15555 Gene_expression denotes overexpression
T8537 15583-15587 Protein denotes MMP9
T8536 15573-15577 Protein denotes IL-6
T8535 15566-15571 Protein denotes IL-1β
T8534 15559-15564 Protein denotes TNF-α
T8533 15510-15515 Protein denotes NF-κB
T8637 18267-18272 Protein denotes NF-κB
T8636 18181-18185 Protein denotes Nrf2
T8635 17837-17851 Positive_regulation denotes overexpression
T8634 17837-17851 Positive_regulation denotes overexpression
T8633 17837-17851 Positive_regulation denotes overexpression
T8632 17837-17851 Gene_expression denotes overexpression
T8631 17837-17851 Gene_expression denotes overexpression
T8630 17837-17851 Gene_expression denotes overexpression
T8629 17937-17941 Protein denotes Nrf2
T8628 17869-17873 Protein denotes IL-6
T8627 17862-17867 Protein denotes IL-1β
T8626 17855-17860 Protein denotes TNF-α
T8625 17707-17716 Negative_regulation denotes Depletion
T8624 17720-17724 Protein denotes Nrf2
T8623 17660-17669 Negative_regulation denotes deficient
T8622 17655-17659 Protein denotes Nrf2
T6758 15296-15300 Protein denotes MMP9
T6757 15134-15138 Protein denotes MMP9
T6756 14965-14969 Protein denotes MMP9
T6755 14851-14859 Positive_regulation denotes elevated
T6754 14828-14838 Gene_expression denotes Expression
T6753 14842-14846 Protein denotes MMP9
T6752 14679-14687 Positive_regulation denotes elevated
T6751 14730-14734 Protein denotes MMP9
T6750 14573-14577 Protein denotes MMP9
T6749 14515-14523 Positive_regulation denotes Enhanced
T6748 14470-14480 Gene_expression denotes Expression
T6747 14527-14531 Protein denotes Nrf2
T6746 14497-14501 Protein denotes MMP9
T5931 14354-14359 Protein denotes IL-1β
T5930 14243-14247 Protein denotes IL-6
T5929 14232-14237 Protein denotes IL-1β
T5928 14223-14227 Protein denotes IL-6
T5927 13952-13963 Positive_regulation denotes upregulated
T5926 13942-13947 Protein denotes TNF-α
T5925 13801-13806 Protein denotes IL-1β
T5924 13757-13762 Protein denotes TNF-α
T5923 13503-13512 Positive_regulation denotes increased
T5922 13503-13512 Positive_regulation denotes increased
T5921 13503-13512 Positive_regulation denotes increased
T5920 13465-13471 Transcription denotes levels
T5919 13465-13471 Transcription denotes levels
T5918 13465-13471 Transcription denotes levels
T5917 13493-13497 Protein denotes IL-6
T5916 13482-13487 Protein denotes IL-1β
T5915 13475-13480 Protein denotes TNF-α
T5914 13360-13364 Protein denotes IL-6
T5913 13349-13354 Protein denotes IL-1β
T5912 13342-13347 Protein denotes TNF-α
T5911 13282-13286 Protein denotes Nrf2
T5910 13175-13184 Negative_regulation denotes deficient
T5909 13170-13174 Protein denotes Nrf2
T5908 12887-12896 Regulation denotes regulated
T5907 12887-12896 Regulation denotes regulated
T5906 12887-12896 Regulation denotes regulated
T5905 12945-12950 Protein denotes NF-κB
T5904 12900-12905 Protein denotes NF-κB
T5903 12878-12882 Protein denotes IL-6
T5902 12867-12872 Protein denotes IL-1β
T5901 12860-12865 Protein denotes TNF-α
T5900 12786-12794 Positive_regulation denotes Elevated
T5899 12798-12802 Protein denotes Nrf2
T4314 10395-10398 Protein denotes Rad
T4313 10115-10118 Protein denotes HRP
T3693 8711-8741 Protein denotes anti-matrix metallopeptidase 9
T3692 8646-8651 Protein denotes actin
T2673 7205-7212 Binding denotes binding
T2672 7195-7200 Protein denotes NF-κB
T2671 6286-6306 Protein denotes bovine serum albumin
T916 2726-2730 Protein denotes Nrf2
T915 2634-2638 Protein denotes Nrf2
T914 2312-2321 Positive_regulation denotes elevating
T314 1170-1176 Positive_regulation denotes induce
T313 1214-1224 Positive_regulation denotes activation
T312 1228-1233 Protein denotes NF-κB
T311 1161-1165 Protein denotes Nrf2
T310 1072-1079 Protein denotes Nrf2 KO
T309 1017-1025 Positive_regulation denotes elevated
T308 975-985 Gene_expression denotes expression
T307 1002-1006 Protein denotes MMP9
T306 936-947 Positive_regulation denotes upregulated
T305 865-870 Protein denotes NF-κB
T304 843-847 Protein denotes MMP9
T303 758-762 Protein denotes MMP9
T302 748-752 Protein denotes IL-6
T301 741-746 Protein denotes IL-1β
T300 734-739 Protein denotes TNF-α
T299 674-679 Protein denotes NF-κB
T298 441-450 Regulation denotes influence
T297 466-476 Positive_regulation denotes activation
T296 480-485 Protein denotes NF-κB
T295 454-458 Protein denotes Nrf2
T294 397-410 Protein denotes Nrf2 wildtype
T293 169-177 Positive_regulation denotes enhances
T292 207-217 Positive_regulation denotes activation
T291 311-316 Protein denotes NF-κB
T290 302-306 Protein denotes Nrf2
T289 221-226 Protein denotes NF-κB
T288 154-158 Protein denotes Nrf2
T5226 12540-12547 Positive_regulation denotes induced
T5225 12548-12558 Positive_regulation denotes activation
T5224 12614-12619 Protein denotes NF-κB
T5223 12562-12567 Protein denotes NF-κB
T5222 12468-12475 Protein denotes Nrf2 WT
T5221 12443-12448 Protein denotes NF-κB
T5220 12364-12373 Positive_regulation denotes activated
T5219 12355-12360 Protein denotes NF-κB
T5218 12256-12264 Positive_regulation denotes Enhanced
T5217 12265-12277 Positive_regulation denotes Upregulation
T5216 12281-12286 Protein denotes NF-κB
T5215 12251-12255 Protein denotes Nrf2
T913 2335-2339 Protein denotes Nrf2
T912 2205-2209 Protein denotes Nrf2
T911 2057-2061 Protein denotes Nrf2
T910 1975-1984 Regulation denotes regulates
T909 1975-1984 Regulation denotes regulates
T908 1894-1936 Protein denotes Nuclear factor erythroid 2-related factor2
T907 1938-1942 Protein denotes Nrf2
T4312 10102-10114 Protein denotes streptavidin
T4311 9596-9602 Gene_expression denotes Levels
T4310 9596-9602 Gene_expression denotes Levels
T4309 9596-9602 Gene_expression denotes Levels
T4308 9596-9602 Gene_expression denotes Levels
T4307 9596-9602 Gene_expression denotes Levels
T4306 9596-9602 Gene_expression denotes Levels
T4305 9690-9694 Protein denotes IL-6
T4304 9675-9688 Protein denotes interleukin-6
T4303 9663-9668 Protein denotes IL-1β
T4302 9643-9661 Protein denotes interleukin-1 beta
T4301 9635-9640 Protein denotes TNF-α
T4300 9606-9633 Protein denotes tumor necrosis factor-alpha
T3696 9328-9335 Protein denotes β-actin
T3695 9004-9026 Protein denotes horseradish peroxidase
T3694 8743-8747 Protein denotes MMP9
T8611 17190-17203 Transcription denotes transcription
T8610 17190-17203 Transcription denotes transcription
T8609 17190-17203 Transcription denotes transcription
T8608 17157-17165 Positive_regulation denotes Enhanced
T8621 17477-17486 Negative_regulation denotes decreases
T8620 17477-17486 Negative_regulation denotes decreases
T8619 17477-17486 Negative_regulation denotes decreases
T8618 17411-17418 Positive_regulation denotes inducer
T8617 17411-17418 Positive_regulation denotes inducer
T8616 17422-17426 Protein denotes Nrf2
T8615 17403-17406 Protein denotes SFN
T8614 17180-17189 Positive_regulation denotes activates
T8613 17180-17189 Positive_regulation denotes activates
T8612 17180-17189 Positive_regulation denotes activates
T8719 20545-20550 Protein denotes TNF-α
T8718 20517-20522 Protein denotes NF-κB
T8717 20486-20490 Protein denotes Nrf2
T8716 20317-20329 Positive_regulation denotes demonstrated
T8715 20317-20329 Positive_regulation denotes demonstrated
T8714 20317-20329 Positive_regulation denotes demonstrated
T8713 20317-20329 Positive_regulation denotes demonstrated
T8712 20334-20345 Positive_regulation denotes upregulated
T8711 20334-20345 Positive_regulation denotes upregulated
T8710 20334-20345 Positive_regulation denotes upregulated
T8709 20334-20345 Positive_regulation denotes upregulated
T8708 20346-20356 Gene_expression denotes expression
T8707 20346-20356 Gene_expression denotes expression
T8706 20346-20356 Gene_expression denotes expression
T8705 20346-20356 Gene_expression denotes expression
T8704 20392-20396 Protein denotes Nrf2
T8703 20384-20388 Protein denotes MMP9
T8702 20374-20378 Protein denotes IL-6
T8701 20367-20372 Protein denotes IL-1β
T8700 20360-20365 Protein denotes TNF-α
T8699 20258-20262 Protein denotes Nrf2
T8698 19824-19834 Gene_expression denotes expression
T8697 19824-19834 Gene_expression denotes expression
T8696 19824-19834 Gene_expression denotes expression
T8695 19912-19925 Protein denotes oxyhemoglobin
T8724 20503-20513 Positive_regulation denotes activation
T8723 20473-20482 Negative_regulation denotes depletion
T8722 20569-20573 Protein denotes MMP9
T8721 20559-20563 Protein denotes IL-6
T8720 20552-20557 Protein denotes IL-1β
T5064 11677-11686 Negative_regulation denotes Depletion
T5063 11690-11694 Protein denotes Nrf2
T4559 11111-11115 Protein denotes MMP9
T3231 7817-7824 Protein denotes β-actin
T3230 7788-7802 Protein denotes ImageJ program
T3229 7555-7577 Protein denotes GoTaq Green Master Mix
T2170 5319-5322 Protein denotes Rad
T2169 5183-5191 Protein denotes μL CCK-8
T1506 4184-4188 Protein denotes GFAP
T1505 3114-3118 Protein denotes Nrf2
T1504 2939-2943 Protein denotes Nrf2
R209 T296 T297 themeOf NF-κB,activation
R210 T297 T298 themeOf activation,influence
R211 T298 T295 Cause influence,Nrf2
R212 T305 T306 themeOf NF-κB,upregulated
R213 T307 T308 themeOf MMP9,expression
R214 T308 T309 themeOf expression,elevated
R215 T312 T313 themeOf NF-κB,activation
R216 T313 T314 themeOf activation,induce
R706 T907 T909 themeOf Nrf2,regulates
R707 T908 T910 themeOf Nuclear factor erythroid 2-related factor2,regulates
R708 T913 T914 themeOf Nrf2,elevating
R2312 T2672 T2673 themeOf NF-κB,binding
R3695 T4300 T4306 themeOf tumor necrosis factor-alpha,Levels
R3696 T4301 T4307 themeOf TNF-α,Levels
R3697 T4302 T4308 themeOf interleukin-1 beta,Levels
R3698 T4303 T4309 themeOf IL-1β,Levels
R3699 T4304 T4310 themeOf interleukin-6,Levels
R3700 T4305 T4311 themeOf IL-6,Levels
R4442 T5219 T5220 themeOf NF-κB,activated
R4443 T5223 T5225 themeOf NF-κB,activation
R4444 T5225 T5226 themeOf activation,induced
R4947 T5899 T5900 themeOf Nrf2,Elevated
R4948 T5901 T5906 themeOf TNF-α,regulated
R4949 T5902 T5907 themeOf IL-1β,regulated
R4950 T5903 T5908 themeOf IL-6,regulated
R4951 T5906 T5904 Cause regulated,NF-κB
R4952 T5907 T5904 Cause regulated,NF-κB
R4953 T5908 T5904 Cause regulated,NF-κB
R4954 T5909 T5910 themeOf Nrf2,deficient
R4955 T5915 T5918 themeOf TNF-α,levels
R4956 T5916 T5919 themeOf IL-1β,levels
R4957 T5917 T5920 themeOf IL-6,levels
R4958 T5918 T5921 themeOf levels,increased
R4959 T5919 T5922 themeOf levels,increased
R4960 T5920 T5923 themeOf levels,increased
R4961 T5926 T5927 themeOf TNF-α,upregulated
R5591 T6746 T6748 themeOf MMP9,Expression
R5592 T6748 T6749 themeOf Expression,Enhanced
R5593 T6751 T6752 themeOf MMP9,elevated
R5594 T6753 T6754 themeOf MMP9,Expression
R5595 T6754 T6755 themeOf Expression,elevated
R6932 T8534 T8538 themeOf TNF-α,overexpression
R6933 T8535 T8539 themeOf IL-1β,overexpression
R6934 T8536 T8540 themeOf IL-6,overexpression
R6935 T8537 T8541 themeOf MMP9,overexpression
R6936 T8538 T8542 themeOf overexpression,overexpression
R6937 T8538 T8546 themeOf overexpression,induced
R6938 T8538 T8554 themeOf overexpression,upregulation
R6939 T8539 T8543 themeOf overexpression,overexpression
R6940 T8539 T8547 themeOf overexpression,induced
R6941 T8539 T8555 themeOf overexpression,upregulation
R6942 T8540 T8544 themeOf overexpression,overexpression
R6943 T8540 T8548 themeOf overexpression,induced
R6944 T8540 T8556 themeOf overexpression,upregulation
R6945 T8541 T8545 themeOf overexpression,overexpression
R6946 T8541 T8549 themeOf overexpression,induced
R6947 T8541 T8557 themeOf overexpression,upregulation
R6948 T8542 T8550 themeOf overexpression,induced
R6949 T8543 T8551 themeOf overexpression,induced
R6950 T8544 T8552 themeOf overexpression,induced
R6951 T8545 T8553 themeOf overexpression,induced
R6952 T8554 T8558 themeOf upregulation,induced
R6953 T8555 T8559 themeOf upregulation,induced
R6954 T8556 T8560 themeOf upregulation,induced
R6955 T8557 T8561 themeOf upregulation,induced
R6956 T8562 T8564 themeOf NF-κB,expression
R6957 T8564 T8565 themeOf expression,induced
R6958 T8566 T8567 themeOf NF-κB,activated
R6959 T8569 T8573 themeOf MMP9,transcription
R6960 T8573 T8574 themeOf transcription,induces
R6961 T8574 T8568 Cause induces,NF-κB
R6998 T8627 T8631 themeOf IL-1β,overexpression
R6999 T8628 T8632 themeOf IL-6,overexpression
R7000 T8630 T8633 themeOf overexpression,overexpression
R7001 T8631 T8634 themeOf overexpression,overexpression
R7002 T8632 T8635 themeOf overexpression,overexpression
R7003 T8639 T8644 themeOf IKK,phosphorylation
R7021 T8664 T8666 themeOf Nrf2,deficient
R7022 T8665 T8667 themeOf activation,Enhanced
R7023 T8667 T8668 themeOf Enhanced,activation
R7024 T8670 T8671 themeOf Nrf2,inhibit
R7025 T8671 T8669 Cause inhibit,NF-κB
R7026 T8676 T8678 themeOf CBP,interaction
R7027 T8679 T8683 themeOf NF-κB,regulated
R7028 T8680 T8684 themeOf Nrf2,regulated
R7029 T8685 T8686 themeOf Nrf2,detected
R7030 T8688 T8690 themeOf Nrf2,activation
R7031 T8689 T8691 themeOf ARE,activation
R7032 T8692 T8696 themeOf TNF-α,expression
R7033 T8693 T8697 themeOf IL-1β,expression
R7034 T8694 T8698 themeOf IL-6,expression
R7035 T8700 T8705 themeOf TNF-α,expression
R7036 T8701 T8706 themeOf IL-1β,expression
R7037 T8702 T8707 themeOf IL-6,expression
R7038 T8703 T8708 themeOf MMP9,expression
R7039 T8705 T8709 themeOf expression,upregulated
R7040 T8706 T8710 themeOf expression,upregulated
R7041 T8707 T8711 themeOf expression,upregulated
R7042 T8708 T8712 themeOf expression,upregulated
R7043 T8709 T8713 themeOf upregulated,demonstrated
R7044 T8710 T8714 themeOf upregulated,demonstrated
R7045 T8711 T8715 themeOf upregulated,demonstrated
R7046 T8712 T8716 themeOf upregulated,demonstrated
R7047 T8717 T8723 themeOf Nrf2,depletion
R7048 T8718 T8724 themeOf NF-κB,activation
R7049 T8719 T8725 themeOf TNF-α,expression
R7050 T8719 T8729 themeOf TNF-α,resulting
R7051 T8720 T8726 themeOf IL-1β,expression
R7052 T8720 T8730 themeOf IL-1β,resulting
R7053 T8721 T8727 themeOf IL-6,expression
R7054 T8721 T8731 themeOf IL-6,resulting
R7055 T8722 T8728 themeOf MMP9,expression
R7056 T8722 T8732 themeOf MMP9,resulting
R7057 T8724 T8733 themeOf activation,induced
R7058 T8725 T8734 themeOf expression,induced
R7060 T8726 T8735 themeOf expression,induced
R7066 T8733 T8723 Cause induced,depletion
R7067 T8734 T8723 Cause induced,depletion
R7068 T8735 T8723 Cause induced,depletion
R4440 T5216 T5217 themeOf NF-κB,Upregulation
R4441 T5217 T5218 themeOf Upregulation,Enhanced
R207 T289 T292 themeOf NF-κB,activation
R208 T292 T293 themeOf activation,enhances
R6962 T8575 T8577 themeOf NF-κB,elevation
R6963 T8576 T8578 themeOf MMP9,overexpression
R6964 T8577 T8580 themeOf elevation,induced
R6965 T8578 T8579 themeOf overexpression,overexpression
R6966 T8578 T8581 themeOf overexpression,induced
R6967 T8578 T8583 themeOf overexpression,elevation
R6968 T8579 T8582 themeOf overexpression,induced
R6969 T8586 T8592 themeOf MMP9,stimulators
R6970 T8586 T8593 themeOf MMP9,stimulators
R6971 T8588 T8594 themeOf MMP9,overexpression
R6972 T8591 T8595 themeOf NF-κB,activation
R6973 T8592 T8584 Cause stimulators,TNF-α
R6974 T8593 T8585 Cause stimulators,IL-1β
R6975 T8594 T8596 themeOf overexpression,induces
R6976 T8595 T8597 themeOf activation,induce
R6977 T8595 T8598 themeOf activation,induce
R6978 T8596 T8587 Cause induces,IL-6
R6979 T8597 T8589 Cause induce,TNF-α
R6980 T8598 T8590 Cause induce,IL-1β
R6981 T8600 T8601 themeOf ARE,regulator
R6982 T8602 T8603 themeOf Nrf2,augmentation
R6983 T8604 T8608 themeOf Nrf2,Enhanced
R6984 T8605 T8609 themeOf heme oxygenase-1,transcription
R6985 T8606 T8610 themeOf NQO1,transcription
R6986 T8607 T8611 themeOf HO-1,transcription
R6987 T8609 T8612 themeOf transcription,activates
R6988 T8610 T8613 themeOf transcription,activates
R6989 T8611 T8614 themeOf transcription,activates
R6990 T8615 T8617 themeOf SFN,inducer
R6991 T8615 T8619 themeOf SFN,decreases
R6992 T8616 T8618 themeOf Nrf2,inducer
R6993 T8617 T8620 themeOf inducer,decreases
R6994 T8618 T8621 themeOf inducer,decreases
R6995 T8622 T8623 themeOf Nrf2,deficient
R6996 T8624 T8625 themeOf Nrf2,Depletion
R6997 T8626 T8630 themeOf TNF-α,overexpression
R7004 T8639 T8647 themeOf IKK,translocation
R7005 T8640 T8645 themeOf IκB,phosphorylation
R7006 T8641 T8648 themeOf p65 NF,translocation
R7007 T8642 T8649 themeOf κB,translocation
R7008 T8644 T8650 themeOf phosphorylation,inhibit
R7009 T8645 T8651 themeOf phosphorylation,inhibit
R7010 T8647 T8646 ToLoc translocation,nuclear
R7011 T8647 T8652 themeOf translocation,inhibit
R7012 T8648 T8646 ToLoc translocation,nuclear
R7013 T8648 T8653 themeOf translocation,inhibit
R7014 T8649 T8646 ToLoc translocation,nuclear
R7015 T8649 T8654 themeOf translocation,inhibit
R7016 T8658 T8660 themeOf Nrf2,depletion
R7017 T8659 T8661 themeOf NF-κB,augmentation
R7018 T8661 T8662 themeOf augmentation,induces
R7019 T8662 T8660 Cause induces,depletion
R7020 T8663 T8665 themeOf NF-κB,activation
R4318 T5063 T5064 themeOf Nrf2,Depletion
R7059 T8725 T8738 themeOf expression,activation
R7061 T8726 T8739 themeOf expression,activation
R7062 T8727 T8736 themeOf expression,induced
R7063 T8727 T8740 themeOf expression,activation
R7064 T8728 T8737 themeOf expression,induced
R7065 T8728 T8741 themeOf expression,activation
R7069 T8736 T8723 Cause induced,depletion
R7070 T8737 T8723 Cause induced,depletion

testone

Id Subject Object Predicate Lexical cue
T7107 20531-20541 Gene_expression denotes expression
T7106 20503-20513 Positive_regulation denotes activation
T7105 20491-20498 Positive_regulation denotes induced
T7104 20473-20482 Negative_regulation denotes depletion
T7103 20397-20405 Negative_regulation denotes knockout
T7102 20346-20356 Gene_expression denotes expression
T7101 20334-20345 Positive_regulation denotes upregulated
T7100 19824-19834 Gene_expression denotes expression
T7099 19403-19412 Regulation denotes regulated
T7098 19250-19256 Entity denotes domain
T7097 19221-19232 Binding denotes interaction
T7096 19150-19159 Negative_regulation denotes repressed
T7095 19093-19100 Negative_regulation denotes inhibit
T7094 19001-19010 Negative_regulation denotes deficient
T7093 18643-18652 Negative_regulation denotes depletion
T7092 18519-18529 Negative_regulation denotes attenuated
T7091 18391-18404 Localization denotes translocation
T7090 18383-18390 Entity denotes nuclear
T7089 18345-18360 Phosphorylation denotes phosphorylation
T7088 18329-18336 Negative_regulation denotes inhibit
T7087 17942-17950 Negative_regulation denotes knockout
T7086 17837-17851 Positive_regulation denotes overexpression
T7085 17707-17716 Negative_regulation denotes Depletion
T7084 17660-17669 Negative_regulation denotes deficient
T7083 15961-15966 Binding denotes binds
T7082 15541-15555 Positive_regulation denotes overexpression
T7081 15494-15506 Positive_regulation denotes upregulation
T7080 15482-15489 Positive_regulation denotes induced
T7079 20569-20573 Protein denotes MMP9
T7078 20559-20563 Protein denotes IL-6
T7077 20552-20557 Protein denotes IL-1β
T7076 20384-20388 Protein denotes MMP9
T7075 20374-20378 Protein denotes IL-6
T7074 20367-20372 Protein denotes IL-1β
T7073 20004-20008 Protein denotes MMP9
T7072 19856-19860 Protein denotes IL-6
T7071 19845-19850 Protein denotes IL-1β
T7070 19138-19141 Protein denotes p65
T7069 18365-18368 Protein denotes p65
T7068 17879-17883 Protein denotes MMP9
T7067 17869-17873 Protein denotes IL-6
T7066 17862-17867 Protein denotes IL-1β
T7065 17263-17267 Protein denotes HO-1
T7064 17245-17261 Protein denotes heme oxygenase-1
T7063 16616-16621 Protein denotes IL-1β
T7062 16560-16564 Protein denotes MMP9
T7061 16529-16533 Protein denotes IL-6
T7060 16496-16500 Protein denotes MMP9
T7059 16463-16468 Protein denotes IL-1β
T7058 16263-16267 Protein denotes MMP9
T7057 16104-16108 Protein denotes IL-6
T7056 16093-16098 Protein denotes IL-1β
T7055 16027-16031 Protein denotes MMP9
T7054 15583-15587 Protein denotes MMP9
T7053 15573-15577 Protein denotes IL-6
T7052 15566-15571 Protein denotes IL-1β
T6453 14828-14838 Gene_expression denotes Expression
T6452 15296-15300 Protein denotes MMP9
T6451 15134-15138 Protein denotes MMP9
T6450 14965-14969 Protein denotes MMP9
T6449 14842-14846 Protein denotes MMP9
T6448 14730-14734 Protein denotes MMP9
T6447 14573-14577 Protein denotes MMP9
T6446 14497-14501 Protein denotes MMP9
T5385 13952-13963 Positive_regulation denotes upregulated
T5384 13503-13512 Positive_regulation denotes increased
T5383 13460-13471 Transcription denotes mRNA levels
T5382 13175-13184 Negative_regulation denotes deficient
T5381 12887-12896 Regulation denotes regulated
T5380 14354-14359 Protein denotes IL-1β
T5379 14243-14247 Protein denotes IL-6
T5378 14232-14237 Protein denotes IL-1β
T5377 14223-14227 Protein denotes IL-6
T5376 13849-13853 Protein denotes IL-6
T5375 13801-13806 Protein denotes IL-1β
T5374 13493-13497 Protein denotes IL-6
T5373 13482-13487 Protein denotes IL-1β
T5372 13360-13364 Protein denotes IL-6
T5371 13349-13354 Protein denotes IL-1β
T5370 12878-12882 Protein denotes IL-6
T5369 12867-12872 Protein denotes IL-1β
T5104 12265-12277 Positive_regulation denotes Upregulation
T5103 12256-12264 Positive_regulation denotes Enhanced
T5102 12237-12247 Negative_regulation denotes Disruption
T4843 11677-11686 Negative_regulation denotes Depletion
T4373 11367-11371 Protein denotes MMP9
T4372 11111-11115 Protein denotes MMP9
T4043 9690-9694 Protein denotes IL-6
T4042 9675-9688 Protein denotes interleukin-6
T4041 9663-9668 Protein denotes IL-1β
T4040 9643-9661 Protein denotes interleukin-1 beta
T3097 7817-7824 Protein denotes β-actin
T3370 8743-8747 Protein denotes MMP9
T2032 5409-5418 Gene_expression denotes expressed
T606 2210-2218 Negative_regulation denotes knockout
T11 1150-1157 Negative_regulation denotes absence
T10 1002-1006 Protein denotes MMP9
T9 843-847 Protein denotes MMP9
T8 758-762 Protein denotes MMP9
T7 748-752 Protein denotes IL-6
T6 741-746 Protein denotes IL-1β
R3486 T4041 T4040 equivalentTo IL-1β,interleukin-1 beta
R3487 T4043 T4042 equivalentTo IL-6,interleukin-6
R4343 T5104 T5103 themeOf Upregulation,Enhanced
R4554 T5369 T5381 themeOf IL-1β,regulated
R4555 T5370 T5381 themeOf IL-6,regulated
R4556 T5373 T5383 themeOf IL-1β,mRNA levels
R4557 T5373 T5384 themeOf IL-1β,increased
R4558 T5374 T5384 themeOf IL-6,increased
R4559 T5374 T5383 themeOf IL-6,mRNA levels
R4560 T5383 T5384 themeOf mRNA levels,increased
R5371 T6449 T6453 themeOf MMP9,Expression
R5809 T7052 T7081 themeOf IL-1β,upregulation
R5810 T7052 T7082 themeOf IL-1β,overexpression
R5811 T7053 T7082 themeOf IL-6,overexpression
R5812 T7054 T7082 themeOf MMP9,overexpression
R5813 T7065 T7064 equivalentTo HO-1,heme oxygenase-1
R5814 T7066 T7086 themeOf IL-1β,overexpression
R5815 T7067 T7086 themeOf IL-6,overexpression
R5816 T7068 T7086 themeOf MMP9,overexpression
R5817 T7069 T7091 themeOf p65,translocation
R5818 T7070 T7097 themeOf p65,interaction
R5819 T7071 T7100 themeOf IL-1β,expression
R5820 T7072 T7100 themeOf IL-6,expression
R5821 T7074 T7102 themeOf IL-1β,expression
R5822 T7075 T7102 themeOf IL-6,expression
R5823 T7076 T7102 themeOf MMP9,expression
R5824 T7077 T7107 themeOf IL-1β,expression
R5825 T7078 T7107 themeOf IL-6,expression
R5826 T7079 T7107 themeOf MMP9,expression
R5827 T7081 T7080 themeOf upregulation,induced
R5828 T7089 T7088 themeOf phosphorylation,inhibit
R5829 T7090 T7091 locationOf nuclear,translocation
R5830 T7091 T7088 themeOf translocation,inhibit
R5831 T7097 T7096 causeOf interaction,repressed
R5832 T7102 T7101 themeOf expression,upregulated
R5833 T7106 T7105 themeOf activation,induced

test3

Id Subject Object Predicate Lexical cue
T608 2312-2321 Positive_regulation denotes elevating
T6465 14573-14577 Protein denotes MMP9
T7121 17263-17267 Protein denotes HO-1
T7120 17245-17261 Protein denotes heme oxygenase-1
T7119 16616-16621 Protein denotes IL-1β
T7118 16560-16564 Protein denotes MMP9
T7117 16529-16533 Protein denotes IL-6
T7116 16496-16500 Protein denotes MMP9
T7115 16463-16468 Protein denotes IL-1β
T7114 16263-16267 Protein denotes MMP9
T7113 16104-16108 Protein denotes IL-6
T7112 16093-16098 Protein denotes IL-1β
T7111 16027-16031 Protein denotes MMP9
T7110 15583-15587 Protein denotes MMP9
T7109 15573-15577 Protein denotes IL-6
T7108 15566-15571 Protein denotes IL-1β
T7193 20569-20573 Protein denotes MMP9
T7192 20559-20563 Protein denotes IL-6
T7191 20552-20557 Protein denotes IL-1β
T7190 20531-20541 Gene_expression denotes expression
T7189 20503-20513 Positive_regulation denotes activation
T7188 20491-20498 Positive_regulation denotes induced
T7187 20473-20482 Negative_regulation denotes depletion
T7186 20397-20405 Negative_regulation denotes knockout
T7185 20384-20388 Protein denotes MMP9
T7184 20374-20378 Protein denotes IL-6
T7183 20367-20372 Protein denotes IL-1β
T7182 20346-20356 Gene_expression denotes expression
T7181 20334-20345 Positive_regulation denotes upregulated
T7180 20004-20008 Protein denotes MMP9
T7179 19856-19860 Protein denotes IL-6
T7178 19845-19850 Protein denotes IL-1β
T7177 19824-19834 Gene_expression denotes expression
T7176 19403-19412 Regulation denotes regulated
T7175 19242-19249 Entity denotes CH1-KIX
T7174 19221-19232 Binding denotes interaction
T7173 19138-19141 Protein denotes p65
T7172 19093-19100 Negative_regulation denotes inhibit
T7171 19001-19010 Negative_regulation denotes deficient
T7170 18391-18404 Localization denotes translocation
T7169 18383-18390 Entity denotes nuclear
T7168 18365-18368 Protein denotes p65
T7167 18345-18360 Phosphorylation denotes phosphorylation
T7166 18329-18336 Negative_regulation denotes inhibit
T7165 18171-18177 Regulation denotes effect
T7164 17942-17950 Negative_regulation denotes knockout
T7163 17879-17883 Protein denotes MMP9
T7162 17869-17873 Protein denotes IL-6
T7161 17862-17867 Protein denotes IL-1β
T7160 17837-17851 Positive_regulation denotes overexpression
T7159 17660-17669 Negative_regulation denotes deficient
T7158 17411-17418 Positive_regulation denotes inducer
T7157 17263-17267 Protein denotes HO-1
T7156 17245-17261 Protein denotes heme oxygenase-1
T7155 16616-16621 Protein denotes IL-1β
T7154 16560-16564 Protein denotes MMP9
T7153 16542-16556 Positive_regulation denotes overexpression
T7152 16534-16541 Positive_regulation denotes induces
T7151 16529-16533 Protein denotes IL-6
T7150 16496-16500 Protein denotes MMP9
T7149 16463-16468 Protein denotes IL-1β
T7148 16263-16267 Protein denotes MMP9
T7147 16245-16259 Positive_regulation denotes overexpression
T7146 16104-16108 Protein denotes IL-6
T7145 16093-16098 Protein denotes IL-1β
T7144 16027-16031 Protein denotes MMP9
T7143 16010-16023 Transcription denotes transcription
T7142 16002-16009 Positive_regulation denotes induces
T7141 15743-15751 Negative_regulation denotes knockout
T7140 15583-15587 Protein denotes MMP9
T7139 15573-15577 Protein denotes IL-6
T7138 15566-15571 Protein denotes IL-1β
T7137 15541-15555 Positive_regulation denotes overexpression
T7136 15520-15527 Binding denotes binding
T7135 20569-20573 Protein denotes MMP9
T7134 20559-20563 Protein denotes IL-6
T7133 20552-20557 Protein denotes IL-1β
T7132 20384-20388 Protein denotes MMP9
T7131 20374-20378 Protein denotes IL-6
T7130 20367-20372 Protein denotes IL-1β
T7129 20004-20008 Protein denotes MMP9
T7128 19856-19860 Protein denotes IL-6
T7127 19845-19850 Protein denotes IL-1β
T7126 19138-19141 Protein denotes p65
T7125 18365-18368 Protein denotes p65
T7124 17879-17883 Protein denotes MMP9
T7123 17869-17873 Protein denotes IL-6
T7122 17862-17867 Protein denotes IL-1β
T6464 14532-14540 Negative_regulation denotes Knockout
T6463 14515-14523 Positive_regulation denotes Enhanced
T6462 14497-14501 Protein denotes MMP9
T6461 14470-14480 Gene_expression denotes Expression
T6460 15296-15300 Protein denotes MMP9
T6459 15134-15138 Protein denotes MMP9
T6458 14965-14969 Protein denotes MMP9
T6457 14842-14846 Protein denotes MMP9
T6456 14730-14734 Protein denotes MMP9
T6455 14573-14577 Protein denotes MMP9
T6454 14497-14501 Protein denotes MMP9
T6472 15296-15300 Protein denotes MMP9
T6471 15134-15138 Protein denotes MMP9
T6470 14965-14969 Protein denotes MMP9
T6469 14851-14859 Positive_regulation denotes elevated
T6468 14842-14846 Protein denotes MMP9
T6467 14828-14838 Gene_expression denotes Expression
T6466 14730-14734 Protein denotes MMP9
T5415 14354-14359 Protein denotes IL-1β
T5414 14243-14247 Protein denotes IL-6
T5413 14232-14237 Protein denotes IL-1β
T5412 14223-14227 Protein denotes IL-6
T5411 13952-13963 Positive_regulation denotes upregulated
T5410 13849-13853 Protein denotes IL-6
T5409 13801-13806 Protein denotes IL-1β
T5408 13503-13512 Positive_regulation denotes increased
T5407 13493-13497 Protein denotes IL-6
T5406 13482-13487 Protein denotes IL-1β
T5405 13360-13364 Protein denotes IL-6
T5404 13349-13354 Protein denotes IL-1β
T5403 13175-13184 Negative_regulation denotes deficient
T5402 12887-12896 Regulation denotes regulated
T5401 12878-12882 Protein denotes IL-6
T5400 12867-12872 Protein denotes IL-1β
T5399 12803-12811 Negative_regulation denotes Knockout
T5398 12786-12794 Positive_regulation denotes Elevated
T5397 14354-14359 Protein denotes IL-1β
T5396 14243-14247 Protein denotes IL-6
T5395 14232-14237 Protein denotes IL-1β
T5394 14223-14227 Protein denotes IL-6
T5393 13849-13853 Protein denotes IL-6
T5392 13801-13806 Protein denotes IL-1β
T5391 13493-13497 Protein denotes IL-6
T5390 13482-13487 Protein denotes IL-1β
T5389 13360-13364 Protein denotes IL-6
T5388 13349-13354 Protein denotes IL-1β
T5387 12878-12882 Protein denotes IL-6
T5386 12867-12872 Protein denotes IL-1β
T4377 11367-11371 Protein denotes MMP9
T4376 11111-11115 Protein denotes MMP9
T4375 11367-11371 Protein denotes MMP9
T4374 11111-11115 Protein denotes MMP9
T4051 9690-9694 Protein denotes IL-6
T4050 9675-9688 Protein denotes interleukin-6
T4049 9663-9668 Protein denotes IL-1β
T4048 9643-9661 Protein denotes interleukin-1 beta
T4047 9690-9694 Protein denotes IL-6
T4046 9675-9688 Protein denotes interleukin-6
T4045 9663-9668 Protein denotes IL-1β
T4044 9643-9661 Protein denotes interleukin-1 beta
T3099 7817-7824 Protein denotes β-actin
T3098 7817-7824 Protein denotes β-actin
T3372 8743-8747 Protein denotes MMP9
T3371 8743-8747 Protein denotes MMP9
T607 2210-2218 Negative_regulation denotes knockout
T22 1150-1157 Negative_regulation denotes absence
T21 1002-1006 Protein denotes MMP9
T20 843-847 Protein denotes MMP9
T19 758-762 Protein denotes MMP9
T18 748-752 Protein denotes IL-6
T17 741-746 Protein denotes IL-1β
T16 1002-1006 Protein denotes MMP9
T15 843-847 Protein denotes MMP9
T14 758-762 Protein denotes MMP9
T13 748-752 Protein denotes IL-6
T12 741-746 Protein denotes IL-1β
R5859 T7179 T7177 themeOf IL-6,expression
R5860 T7182 T7181 themeOf expression,upregulated
R5861 T7183 T7182 themeOf IL-1β,expression
R5862 T7184 T7182 themeOf IL-6,expression
R5863 T7185 T7182 themeOf MMP9,expression
R5864 T7189 T7188 themeOf activation,induced
R5865 T7191 T7190 themeOf IL-1β,expression
R5866 T7192 T7190 themeOf IL-6,expression
R5867 T7193 T7190 themeOf MMP9,expression
R3488 T4049 T4048 equivalentTo IL-1β,interleukin-1 beta
R3489 T4051 T4050 equivalentTo IL-6,interleukin-6
R4561 T5400 T5402 themeOf IL-1β,regulated
R4562 T5401 T5402 themeOf IL-6,regulated
R4563 T5407 T5408 themeOf IL-6,increased
R5372 T6461 T6463 themeOf Expression,Enhanced
R5373 T6467 T6469 themeOf Expression,elevated
R5374 T6468 T6467 themeOf MMP9,Expression
R5834 T7138 T7137 themeOf IL-1β,overexpression
R5835 T7138 T7136 themeOf IL-1β,binding
R5836 T7139 T7137 themeOf IL-6,overexpression
R5837 T7139 T7136 themeOf IL-6,binding
R5838 T7140 T7136 themeOf MMP9,binding
R5839 T7140 T7137 themeOf MMP9,overexpression
R5840 T7143 T7142 themeOf transcription,induces
R5841 T7144 T7143 themeOf MMP9,transcription
R5842 T7145 T7143 themeOf IL-1β,transcription
R5843 T7146 T7143 themeOf IL-6,transcription
R5844 T7148 T7147 themeOf MMP9,overexpression
R5845 T7151 T7152 causeOf IL-6,induces
R5846 T7153 T7152 themeOf overexpression,induces
R5847 T7154 T7153 themeOf MMP9,overexpression
R5848 T7157 T7156 equivalentTo HO-1,heme oxygenase-1
R5849 T7161 T7160 themeOf IL-1β,overexpression
R5850 T7162 T7160 themeOf IL-6,overexpression
R5851 T7163 T7160 themeOf MMP9,overexpression
R5852 T7167 T7166 themeOf phosphorylation,inhibit
R5853 T7168 T7170 themeOf p65,translocation
R5854 T7169 T7170 locationOf nuclear,translocation
R5855 T7170 T7166 themeOf translocation,inhibit
R5856 T7173 T7174 themeOf p65,interaction
R5857 T7175 T7174 themeOf CH1-KIX,interaction
R5858 T7178 T7177 themeOf IL-1β,expression