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PMC:3312845 / 9314-10106 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T4571 784-791 NN denotes animals
T4570 779-783 DT denotes some
T4569 776-778 IN denotes in
T4568 771-775 VB denotes used
T4567 767-770 VB denotes was
T4566 755-766 NN denotes hippocampus
T4565 748-754 JJ denotes entire
T4564 744-747 DT denotes the
T4563 733-743 IN denotes throughout
T4562 726-732 NN denotes series
T4561 722-725 DT denotes the
T4460 148-152 VB denotes were
T4459 140-147 NN denotes animals
T4458 138-139 -COMMA- denotes ,
T4457 137-138 -RRB- denotes )
T4456 132-137 NN denotes point
T4455 127-131 NN denotes time
T4454 122-126 DT denotes each
T4453 118-121 IN denotes for
T4452 116-117 -COMMA- denotes ,
T4451 115-116 CD denotes 5
T4450 113-114 SYM denotes =
T4449 111-112 NN denotes n
T4448 109-110 -COLON- denotes ;
T4447 107-109 NN denotes SE
T4446 101-106 IN denotes after
T4445 96-100 NN denotes week
T4444 94-95 CD denotes 1
T4443 90-93 CC denotes and
T4442 85-89 NN denotes days
T4441 83-84 CD denotes 4
T4440 81-82 -COMMA- denotes ,
T4439 77-81 NN denotes days
T4438 75-76 CD denotes 3
T4437 73-74 -COMMA- denotes ,
T4436 69-73 NN denotes days
T4435 67-68 CD denotes 2
T4434 65-66 -COMMA- denotes ,
T4433 62-65 NN denotes day
T4432 60-61 CD denotes 1
T4431 58-59 -COMMA- denotes ,
T4430 56-58 NN denotes hr
T4429 53-55 CD denotes 12
T4428 51-52 -COLON- denotes :
T4427 45-51 JJ denotes non-SE
T4426 44-45 -LRB- denotes (
T4425 37-43 NN denotes points
T4424 32-36 NN denotes time
T4423 21-31 VB denotes designated
T4422 18-20 IN denotes At
T4421 7-17 NN denotes processing
T4420 0-6 NN denotes Tissue
T4560 719-721 IN denotes in
T4559 711-718 NN denotes section
T4558 705-710 JJ denotes sixth
T4557 699-704 DT denotes every
T4556 697-698 -COMMA- denotes ,
T4555 692-697 NN denotes study
T4554 678-691 JJ denotes stereological
T4553 674-677 IN denotes For
T4552 669-672 NNP denotes PBS
T4551 658-668 VB denotes containing
T4550 651-657 NN denotes plates
T4549 642-650 JJ denotes six-well
T4548 639-641 IN denotes in
T4547 629-638 VB denotes contained
T4546 624-628 VB denotes were
T4545 615-623 NN denotes sections
T4544 603-614 JJ denotes consecutive
T4543 599-602 CC denotes and
T4542 596-598 NN denotes μm
T4541 593-595 CD denotes 30
T4540 590-592 IN denotes at
T4539 581-589 NN denotes cryostat
T4538 579-580 DT denotes a
T4537 574-578 IN denotes with
T4536 564-573 VB denotes sectioned
T4535 560-563 CC denotes and
T4534 553-559 VB denotes frozen
T4533 549-552 VB denotes was
T4532 537-548 NN denotes hippocampus
T4531 530-536 JJ denotes entire
T4530 526-529 DT denotes the
T4529 524-525 -COMMA- denotes ,
T4528 514-524 RB denotes Thereafter
T4527 503-512 RB denotes overnight
T4526 495-502 NN denotes sucrose
T4525 493-494 NN denotes %
T4524 491-493 CD denotes 30
T4523 486-490 IN denotes with
T4522 473-485 NN denotes infiltration
T4521 470-472 IN denotes by
T4520 456-469 VB denotes cryoprotected
T4519 451-455 VB denotes were
T4518 443-450 NN denotes tissues
T4517 437-442 NN denotes brain
T4516 433-436 DT denotes The
T4515 429-431 NN denotes hr
T4514 427-428 CD denotes 4
T4513 423-426 IN denotes for
T4512 414-422 JJ denotes fixative
T4511 409-413 JJ denotes same
T4510 405-408 DT denotes the
T4509 402-404 IN denotes in
T4508 392-401 VB denotes postfixed
T4507 388-391 CC denotes and
T4506 386-387 -COMMA- denotes ,
T4505 379-386 VB denotes removed
T4504 374-378 VB denotes were
T4503 367-373 NN denotes brains
T4502 363-366 DT denotes The
T4501 360-361 -RRB- denotes )
T4500 358-360 NN denotes MO
T4499 356-357 -COMMA- denotes ,
T4498 351-356 NNP denotes Louis
T4497 347-350 NNP denotes St.
T4496 345-346 -COMMA- denotes ,
T4495 342-345 NNP denotes Co.
T4494 328-341 NNP denotes Sigma-Aldrich
T4493 326-327 -COLON- denotes ;
T4492 322-326 RB denotes i.p.
T4491 320-321 -COMMA- denotes ,
T4490 316-320 NN denotes g/kg
T4489 312-315 CD denotes 1.5
T4488 311-312 -LRB- denotes (
T4487 300-310 NN denotes anesthesia
T4486 291-299 NN denotes urethane
T4485 285-290 IN denotes under
T4484 283-284 -RRB- denotes )
T4483 280-283 CD denotes 7.4
T4482 277-279 NN denotes pH
T4481 275-276 -COMMA- denotes ,
T4480 273-275 NN denotes PB
T4479 272-273 -LRB- denotes (
T4478 265-271 NN denotes buffer
T4477 255-264 NN denotes phosphate
T4476 253-254 NN denotes M
T4475 249-252 CD denotes 0.1
T4474 246-248 IN denotes in
T4473 229-245 NN denotes paraformaldehyde
T4472 227-228 NN denotes %
T4471 226-227 CD denotes 4
T4470 223-225 IN denotes by
T4469 214-222 VB denotes followed
T4468 212-213 -RRB- denotes )
T4467 209-212 NN denotes PBS
T4466 208-209 -LRB- denotes (
T4465 201-207 NN denotes saline
T4464 182-200 JJ denotes phosphate-buffered
T4463 177-181 IN denotes with
T4462 162-176 RB denotes transcardially
T4461 153-161 VB denotes perfused
R3468 T4425 T4422 arg2Of points,At
R3469 T4425 T4423 arg2Of points,designated
R3470 T4425 T4424 arg1Of points,time
R3471 T4425 T4426 arg1Of points,(
R3472 T4427 T4426 arg2Of non-SE,(
R3473 T4428 T4426 arg3Of :,(
R3474 T4430 T4429 arg1Of hr,12
R3475 T4430 T4431 arg1Of hr,","
R3476 T4430 T4434 arg1Of hr,","
R3477 T4430 T4437 arg1Of hr,","
R3478 T4430 T4440 arg1Of hr,","
R3479 T4433 T4431 arg2Of day,","
R3480 T4433 T4432 arg1Of day,1
R3481 T4436 T4434 arg2Of days,","
R3482 T4436 T4435 arg1Of days,2
R3483 T4439 T4437 arg2Of days,","
R3484 T4439 T4438 arg1Of days,3
R3485 T4440 T4443 arg1Of ",",and
R3486 T4442 T4440 arg2Of days,","
R3487 T4442 T4441 arg1Of days,4
R3488 T4443 T4460 arg1Of and,were
R3489 T4443 T4461 arg2Of and,perfused
R3490 T4445 T4443 arg2Of week,and
R3491 T4445 T4444 arg1Of week,1
R3492 T4445 T4446 arg1Of week,after
R3493 T4445 T4448 arg1Of week,;
R3494 T4447 T4446 arg2Of SE,after
R3495 T4449 T4448 arg2Of n,;
R3496 T4449 T4450 arg1Of n,=
R3497 T4450 T4451 arg1Of =,5
R3498 T4450 T4452 arg1Of =,","
R3499 T4450 T4453 arg1Of =,for
R3500 T4450 T4458 arg1Of =,","
R3501 T4456 T4453 arg2Of point,for
R3502 T4456 T4454 arg1Of point,each
R3503 T4456 T4455 arg1Of point,time
R3504 T4456 T4457 arg1Of point,)
R3505 T4459 T4458 arg2Of animals,","
R3506 T4461 T4460 arg2Of perfused,were
R3507 T4461 T4462 arg1Of perfused,transcardially
R3508 T4461 T4463 arg1Of perfused,with
R3509 T4465 T4463 arg2Of saline,with
R3510 T4465 T4464 arg1Of saline,phosphate-buffered
R3511 T4465 T4466 arg1Of saline,(
R3512 T4465 T4469 arg2Of saline,followed
R3513 T4467 T4466 arg2Of PBS,(
R3514 T4468 T4466 arg3Of ),(
R3515 T4471 T4472 arg1Of 4,%
R3516 T4473 T4469 arg1Of paraformaldehyde,followed
R3517 T4473 T4470 arg2Of paraformaldehyde,by
R3518 T4473 T4471 arg1Of paraformaldehyde,4
R3519 T4473 T4474 arg1Of paraformaldehyde,in
R3520 T4475 T4476 arg1Of 0.1,M
R3521 T4478 T4474 arg2Of buffer,in
R3522 T4478 T4475 arg1Of buffer,0.1
R3523 T4478 T4477 arg1Of buffer,phosphate
R3524 T4478 T4479 arg1Of buffer,(
R3525 T4478 T4485 arg1Of buffer,under
R3526 T4478 T4488 arg1Of buffer,(
R3527 T4480 T4479 arg2Of PB,(
R3528 T4480 T4481 arg1Of PB,","
R3529 T4482 T4481 arg2Of pH,","
R3530 T4482 T4483 arg1Of pH,7.4
R3531 T4484 T4479 arg3Of ),(
R3532 T4487 T4485 arg2Of anesthesia,under
R3533 T4487 T4486 arg1Of anesthesia,urethane
R3534 T4490 T4489 arg1Of g/kg,1.5
R3535 T4490 T4491 arg1Of g/kg,","
R3536 T4490 T4492 arg1Of g/kg,i.p.
R3537 T4490 T4493 arg1Of g/kg,;
R3538 T4493 T4496 arg1Of ;,","
R3539 T4495 T4493 arg2Of Co.,;
R3540 T4495 T4494 arg1Of Co.,Sigma-Aldrich
R3541 T4496 T4488 arg2Of ",",(
R3542 T4496 T4499 arg1Of ",",","
R3543 T4498 T4496 arg2Of Louis,","
R3544 T4498 T4497 arg1Of Louis,St.
R3545 T4500 T4499 arg2Of MO,","
R3546 T4501 T4488 arg3Of ),(
R3547 T4503 T4502 arg1Of brains,The
R3548 T4503 T4504 arg1Of brains,were
R3549 T4503 T4505 arg2Of brains,removed
R3550 T4503 T4508 arg2Of brains,postfixed
R3551 T4505 T4507 arg1Of removed,and
R3552 T4507 T4504 arg2Of and,were
R3553 T4507 T4506 arg1Of and,","
R3554 T4508 T4507 arg2Of postfixed,and
R3555 T4508 T4509 arg1Of postfixed,in
R3556 T4508 T4513 arg1Of postfixed,for
R3557 T4512 T4509 arg2Of fixative,in
R3558 T4512 T4510 arg1Of fixative,the
R3559 T4512 T4511 arg1Of fixative,same
R3560 T4515 T4513 arg2Of hr,for
R3561 T4515 T4514 arg1Of hr,4
R3562 T4518 T4516 arg1Of tissues,The
R3563 T4518 T4517 arg1Of tissues,brain
R3564 T4518 T4519 arg1Of tissues,were
R3565 T4518 T4520 arg2Of tissues,cryoprotected
R3566 T4520 T4519 arg2Of cryoprotected,were
R3567 T4522 T4520 arg1Of infiltration,cryoprotected
R3568 T4522 T4521 arg2Of infiltration,by
R3569 T4522 T4523 arg1Of infiltration,with
R3570 T4524 T4525 arg1Of 30,%
R3571 T4526 T4523 arg2Of sucrose,with
R3572 T4526 T4524 arg1Of sucrose,30
R3573 T4526 T4527 arg1Of sucrose,overnight
R3574 T4532 T4530 arg1Of hippocampus,the
R3575 T4532 T4531 arg1Of hippocampus,entire
R3576 T4532 T4533 arg1Of hippocampus,was
R3577 T4532 T4534 arg2Of hippocampus,frozen
R3578 T4532 T4536 arg2Of hippocampus,sectioned
R3579 T4534 T4535 arg1Of frozen,and
R3580 T4535 T4528 arg1Of and,Thereafter
R3581 T4535 T4529 arg1Of and,","
R3582 T4535 T4533 arg2Of and,was
R3583 T4535 T4540 arg1Of and,at
R3584 T4535 T4543 arg1Of and,and
R3585 T4536 T4535 arg2Of sectioned,and
R3586 T4536 T4537 arg1Of sectioned,with
R3587 T4539 T4537 arg2Of cryostat,with
R3588 T4539 T4538 arg1Of cryostat,a
R3589 T4542 T4540 arg2Of μm,at
R3590 T4542 T4541 arg1Of μm,30
R3591 T4545 T4544 arg1Of sections,consecutive
R3592 T4545 T4546 arg1Of sections,were
R3593 T4545 T4547 arg2Of sections,contained
R3594 T4547 T4543 arg2Of contained,and
R3595 T4547 T4546 arg2Of contained,were
R3596 T4547 T4548 arg1Of contained,in
R3597 T4550 T4548 arg2Of plates,in
R3598 T4550 T4549 arg1Of plates,six-well
R3599 T4550 T4551 arg1Of plates,containing
R3600 T4552 T4551 arg2Of PBS,containing
R3601 T4555 T4553 arg2Of study,For
R3602 T4555 T4554 arg1Of study,stereological
R3603 T4559 T4557 arg1Of section,every
R3604 T4559 T4558 arg1Of section,sixth
R3605 T4559 T4560 arg1Of section,in
R3606 T4559 T4567 arg1Of section,was
R3607 T4559 T4568 arg2Of section,used
R3608 T4562 T4560 arg2Of series,in
R3609 T4562 T4561 arg1Of series,the
R3610 T4562 T4563 arg1Of series,throughout
R3611 T4566 T4563 arg2Of hippocampus,throughout
R3612 T4566 T4564 arg1Of hippocampus,the
R3613 T4566 T4565 arg1Of hippocampus,entire
R3614 T4568 T4553 arg1Of used,For
R3615 T4568 T4556 arg1Of used,","
R3616 T4568 T4567 arg2Of used,was
R3617 T4568 T4569 arg1Of used,in
R3618 T4571 T4569 arg2Of animals,in
R3619 T4571 T4570 arg1Of animals,some
R3467 T4421 T4420 arg1Of processing,Tissue

UBERON-AE

Id Subject Object Predicate Lexical cue
T4412 437-442 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain
T4411 367-373 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brains
T4410 443-450 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissues
T4409 0-6 http://purl.obolibrary.org/obo/UBERON_0000479 denotes Tissue

sentences

Id Subject Object Predicate Lexical cue
T4419 674-792 Sentence denotes For stereological study, every sixth section in the series throughout the entire hippocampus was used in some animals.
T4418 514-673 Sentence denotes Thereafter, the entire hippocampus was frozen and sectioned with a cryostat at 30 μm and consecutive sections were contained in six-well plates containing PBS.
T4417 433-513 Sentence denotes The brain tissues were cryoprotected by infiltration with 30% sucrose overnight.
T4416 363-432 Sentence denotes The brains were removed, and postfixed in the same fixative for 4 hr.
T4415 53-362 Sentence denotes 12 hr, 1 day, 2 days, 3 days, 4 days and 1 week after SE; n = 5, for each time point), animals were perfused transcardially with phosphate-buffered saline (PBS) followed by 4% paraformaldehyde in 0.1 M phosphate buffer (PB, pH 7.4) under urethane anesthesia (1.5 g/kg, i.p.; Sigma-Aldrich Co., St. Louis, MO).
T4414 18-52 Sentence denotes At designated time points (non-SE:
T4413 0-17 Sentence denotes Tissue processing
T74 0-17 Sentence denotes Tissue processing
T75 18-52 Sentence denotes At designated time points (non-SE:
T76 53-362 Sentence denotes 12 hr, 1 day, 2 days, 3 days, 4 days and 1 week after SE; n = 5, for each time point), animals were perfused transcardially with phosphate-buffered saline (PBS) followed by 4% paraformaldehyde in 0.1 M phosphate buffer (PB, pH 7.4) under urethane anesthesia (1.5 g/kg, i.p.; Sigma-Aldrich Co., St. Louis, MO).
T77 363-432 Sentence denotes The brains were removed, and postfixed in the same fixative for 4 hr.
T78 433-513 Sentence denotes The brain tissues were cryoprotected by infiltration with 30% sucrose overnight.
T79 514-673 Sentence denotes Thereafter, the entire hippocampus was frozen and sectioned with a cryostat at 30 μm and consecutive sections were contained in six-well plates containing PBS.
T80 674-792 Sentence denotes For stereological study, every sixth section in the series throughout the entire hippocampus was used in some animals.

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T4739 223-245 Entity denotes by 4% paraformaldehyde
T4738 328-357 Protein denotes Sigma-Aldrich Co., St. Louis,
T4737 45-109 Protein denotes non-SE: 12 hr, 1 day, 2 days, 3 days, 4 days and 1 week after SE
T4736 291-310 Entity denotes urethane anesthesia
T4735 255-264 Entity denotes phosphate
T4734 182-213 Entity denotes phosphate-buffered saline (PBS)
T4733 111-114 Entity denotes n =
T4732 182-200 Entity denotes phosphate-buffered
T4731 491-512 Entity denotes 30% sucrose overnight
T4730 405-431 Entity denotes the same fixative for 4 hr
T4729 273-275 Protein denotes PB

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T4728 791-792 . denotes .
T4727 784-791 NNS denotes animals
T4726 779-783 DT denotes some
T4725 776-778 IN denotes in
T4724 771-775 VBN denotes used
T4723 767-770 VBD denotes was
T4653 360-361 -RRB- denotes )
T4652 358-360 NNP denotes MO
T4651 356-357 , denotes ,
T4650 351-356 NNP denotes Louis
T4649 347-350 NNP denotes St.
T4648 345-346 , denotes ,
T4647 342-345 NNP denotes Co.
T4646 328-341 NNP denotes Sigma-Aldrich
T4645 326-327 : denotes ;
T4644 322-326 NN denotes i.p.
T4643 320-321 , denotes ,
T4642 316-320 NN denotes g/kg
T4641 312-315 CD denotes 1.5
T4640 311-312 -LRB- denotes (
T4639 300-310 NN denotes anesthesia
T4638 291-299 NN denotes urethane
T4637 285-290 IN denotes under
T4636 283-284 -RRB- denotes )
T4635 280-283 CD denotes 7.4
T4634 277-279 NNP denotes pH
T4633 275-276 , denotes ,
T4632 273-275 NNP denotes PB
T4631 272-273 -LRB- denotes (
T4630 265-271 NN denotes buffer
T4629 255-264 NN denotes phosphate
T4628 253-254 NNP denotes M
T4627 249-252 CD denotes 0.1
T4626 246-248 IN denotes in
T4625 229-245 NN denotes paraformaldehyde
T4624 227-228 NN denotes %
T4623 226-227 CD denotes 4
T4622 223-225 IN denotes by
T4621 214-222 VBN denotes followed
T4620 212-213 -RRB- denotes )
T4619 209-212 NNP denotes PBS
T4618 208-209 -LRB- denotes (
T4617 201-207 NN denotes saline
T4616 182-200 JJ denotes phosphate-buffered
T4615 177-181 IN denotes with
T4614 162-176 RB denotes transcardially
T4613 153-161 JJ denotes perfused
T4612 148-152 VBD denotes were
T4611 140-147 NNS denotes animals
T4610 138-139 , denotes ,
T4609 137-138 -RRB- denotes )
T4608 132-137 NN denotes point
T4607 127-131 NN denotes time
T4606 122-126 DT denotes each
T4605 118-121 IN denotes for
T4604 116-117 , denotes ,
T4603 115-116 CD denotes 5
T4602 113-114 SYM denotes =
T4601 111-112 VB denotes n
T4600 109-110 : denotes ;
T4599 107-109 NNP denotes SE
T4598 101-106 IN denotes after
T4597 96-100 NN denotes week
T4596 94-95 CD denotes 1
T4595 90-93 CC denotes and
T4594 85-89 NNS denotes days
T4593 83-84 CD denotes 4
T4592 81-82 , denotes ,
T4591 77-81 NNS denotes days
T4590 75-76 CD denotes 3
T4589 73-74 , denotes ,
T4588 69-73 NNS denotes days
T4587 67-68 CD denotes 2
T4586 65-66 , denotes ,
T4585 62-65 NN denotes day
T4584 60-61 CD denotes 1
T4583 58-59 , denotes ,
T4582 56-58 NN denotes hr
T4581 53-55 CD denotes 12
T4580 51-52 : denotes :
T4579 45-51 JJ denotes non-SE
T4578 44-45 -LRB- denotes (
T4577 37-43 NNS denotes points
T4576 32-36 NN denotes time
T4575 21-31 VBN denotes designated
T4574 18-20 IN denotes At
T4573 7-17 NN denotes processing
T4572 0-6 NN denotes Tissue
T4700 615-623 NNS denotes sections
T4699 603-614 JJ denotes consecutive
T4698 599-602 CC denotes and
T4697 596-598 NN denotes μm
T4696 593-595 CD denotes 30
T4695 590-592 IN denotes at
T4694 581-589 NN denotes cryostat
T4693 579-580 DT denotes a
T4692 574-578 IN denotes with
T4691 564-573 VBN denotes sectioned
T4690 560-563 CC denotes and
T4689 553-559 VBN denotes frozen
T4688 549-552 VBD denotes was
T4687 537-548 NN denotes hippocampus
T4686 530-536 JJ denotes entire
T4685 526-529 DT denotes the
T4684 524-525 , denotes ,
T4683 514-524 RB denotes Thereafter
T4682 512-513 . denotes .
T4681 503-512 JJ denotes overnight
T4680 495-502 JJ denotes sucrose
T4679 493-494 NN denotes %
T4678 491-493 CD denotes 30
T4677 486-490 IN denotes with
T4676 473-485 NN denotes infiltration
T4675 470-472 IN denotes by
T4674 456-469 VBN denotes cryoprotected
T4673 451-455 VBD denotes were
T4672 443-450 NNS denotes tissues
T4671 437-442 NN denotes brain
T4670 433-436 DT denotes The
T4669 431-432 . denotes .
T4668 429-431 NN denotes hr
T4667 427-428 CD denotes 4
T4666 423-426 IN denotes for
T4665 414-422 NN denotes fixative
T4664 409-413 JJ denotes same
T4663 405-408 DT denotes the
T4662 402-404 IN denotes in
T4661 392-401 VBD denotes postfixed
T4660 388-391 CC denotes and
T4659 386-387 , denotes ,
T4658 379-386 VBN denotes removed
T4657 374-378 VBD denotes were
T4656 367-373 NNS denotes brains
T4655 363-366 DT denotes The
T4654 361-362 . denotes .
T4702 629-638 VBN denotes contained
T4701 624-628 VBD denotes were
T4722 755-766 NN denotes hippocampus
T4721 748-754 JJ denotes entire
T4720 744-747 DT denotes the
T4719 733-743 IN denotes throughout
T4718 726-732 NN denotes series
T4717 722-725 DT denotes the
T4716 719-721 IN denotes in
T4715 711-718 NN denotes section
T4714 705-710 JJ denotes sixth
T4713 699-704 DT denotes every
T4712 697-698 , denotes ,
T4711 692-697 NN denotes study
T4710 678-691 JJ denotes stereological
T4709 674-677 IN denotes For
T4708 672-673 . denotes .
T4707 669-672 NNP denotes PBS
T4706 658-668 VBG denotes containing
T4705 651-657 NNS denotes plates
T4704 642-650 JJ denotes six-well
T4703 639-641 IN denotes in
R3620 T4572 T4573 compound Tissue,processing
R3621 T4573 T4573 ROOT processing,processing
R3622 T4574 T4621 prep At,followed
R3623 T4575 T4577 amod designated,points
R3624 T4576 T4577 compound time,points
R3625 T4577 T4574 pobj points,At
R3626 T4578 T4582 punct (,hr
R3627 T4579 T4582 amod non-SE,hr
R3628 T4580 T4582 punct :,hr
R3629 T4581 T4582 nummod 12,hr
R3630 T4582 T4621 nsubjpass hr,followed
R3631 T4583 T4582 punct ",",hr
R3632 T4584 T4585 nummod 1,day
R3633 T4585 T4582 appos day,hr
R3634 T4586 T4585 punct ",",day
R3635 T4587 T4588 nummod 2,days
R3636 T4588 T4585 appos days,day
R3637 T4589 T4588 punct ",",days
R3638 T4590 T4591 nummod 3,days
R3639 T4591 T4588 appos days,days
R3640 T4592 T4591 punct ",",days
R3641 T4593 T4594 nummod 4,days
R3642 T4594 T4591 conj days,days
R3643 T4595 T4594 cc and,days
R3644 T4596 T4597 nummod 1,week
R3645 T4597 T4585 appos week,day
R3646 T4598 T4597 prep after,week
R3647 T4599 T4598 pobj SE,after
R3648 T4600 T4582 punct ;,hr
R3649 T4601 T4612 advcl n,were
R3650 T4602 T4603 punct =,5
R3651 T4603 T4601 nummod 5,n
R3652 T4604 T4601 punct ",",n
R3653 T4605 T4601 prep for,n
R3654 T4606 T4607 det each,time
R3655 T4607 T4608 compound time,point
R3656 T4608 T4605 pobj point,for
R3657 T4609 T4601 punct ),n
R3658 T4610 T4612 punct ",",were
R3659 T4611 T4612 nsubj animals,were
R3660 T4612 T4621 auxpass were,followed
R3661 T4613 T4612 acomp perfused,were
R3662 T4614 T4612 advmod transcardially,were
R3663 T4615 T4612 prep with,were
R3664 T4616 T4617 amod phosphate-buffered,saline
R3665 T4617 T4615 pobj saline,with
R3666 T4618 T4619 punct (,PBS
R3667 T4619 T4617 appos PBS,saline
R3668 T4620 T4612 punct ),were
R3669 T4621 T4647 ccomp followed,Co.
R3670 T4622 T4621 agent by,followed
R3671 T4623 T4624 nummod 4,%
R3672 T4624 T4622 pobj %,by
R3673 T4625 T4621 npadvmod paraformaldehyde,followed
R3674 T4626 T4621 prep in,followed
R3675 T4627 T4630 nummod 0.1,buffer
R3676 T4628 T4630 compound M,buffer
R3677 T4629 T4630 compound phosphate,buffer
R3678 T4630 T4626 pobj buffer,in
R3679 T4631 T4632 punct (,PB
R3680 T4632 T4630 appos PB,buffer
R3681 T4633 T4632 punct ",",PB
R3682 T4634 T4632 appos pH,PB
R3683 T4635 T4634 nummod 7.4,pH
R3684 T4636 T4632 punct ),PB
R3685 T4637 T4621 prep under,followed
R3686 T4638 T4639 compound urethane,anesthesia
R3687 T4639 T4637 pobj anesthesia,under
R3688 T4640 T4639 punct (,anesthesia
R3689 T4641 T4642 nummod 1.5,g/kg
R3690 T4642 T4639 appos g/kg,anesthesia
R3691 T4643 T4642 punct ",",g/kg
R3692 T4644 T4642 conj i.p.,g/kg
R3693 T4645 T4647 punct ;,Co.
R3694 T4646 T4647 compound Sigma-Aldrich,Co.
R3695 T4647 T4647 ROOT Co.,Co.
R3696 T4648 T4647 punct ",",Co.
R3697 T4649 T4650 compound St.,Louis
R3698 T4650 T4647 npadvmod Louis,Co.
R3699 T4651 T4647 punct ",",Co.
R3700 T4652 T4647 appos MO,Co.
R3701 T4653 T4647 punct ),Co.
R3702 T4654 T4647 punct .,Co.
R3703 T4655 T4656 det The,brains
R3704 T4656 T4658 nsubjpass brains,removed
R3705 T4657 T4658 auxpass were,removed
R3706 T4658 T4658 ROOT removed,removed
R3707 T4659 T4658 punct ",",removed
R3708 T4660 T4658 cc and,removed
R3709 T4661 T4658 conj postfixed,removed
R3710 T4662 T4661 prep in,postfixed
R3711 T4663 T4665 det the,fixative
R3712 T4664 T4665 amod same,fixative
R3713 T4665 T4662 pobj fixative,in
R3714 T4666 T4661 prep for,postfixed
R3715 T4667 T4668 nummod 4,hr
R3716 T4668 T4666 pobj hr,for
R3717 T4669 T4658 punct .,removed
R3718 T4670 T4672 det The,tissues
R3719 T4671 T4672 compound brain,tissues
R3720 T4672 T4674 nsubjpass tissues,cryoprotected
R3721 T4673 T4674 auxpass were,cryoprotected
R3722 T4674 T4674 ROOT cryoprotected,cryoprotected
R3723 T4675 T4674 agent by,cryoprotected
R3724 T4676 T4675 pobj infiltration,by
R3725 T4677 T4676 prep with,infiltration
R3726 T4678 T4679 nummod 30,%
R3727 T4679 T4681 nmod %,overnight
R3728 T4680 T4681 amod sucrose,overnight
R3729 T4681 T4677 pobj overnight,with
R3730 T4682 T4674 punct .,cryoprotected
R3731 T4683 T4689 advmod Thereafter,frozen
R3732 T4684 T4689 punct ",",frozen
R3733 T4685 T4687 det the,hippocampus
R3734 T4686 T4687 amod entire,hippocampus
R3735 T4687 T4689 nsubjpass hippocampus,frozen
R3736 T4688 T4689 auxpass was,frozen
R3737 T4689 T4689 ROOT frozen,frozen
R3738 T4690 T4689 cc and,frozen
R3739 T4691 T4689 conj sectioned,frozen
R3740 T4692 T4691 prep with,sectioned
R3741 T4693 T4694 det a,cryostat
R3742 T4694 T4692 pobj cryostat,with
R3743 T4695 T4694 prep at,cryostat
R3744 T4696 T4697 nummod 30,μm
R3745 T4697 T4695 pobj μm,at
R3746 T4698 T4697 cc and,μm
R3747 T4699 T4700 amod consecutive,sections
R3748 T4700 T4702 nsubjpass sections,contained
R3749 T4701 T4702 auxpass were,contained
R3750 T4702 T4689 conj contained,frozen
R3751 T4703 T4702 prep in,contained
R3752 T4704 T4705 amod six-well,plates
R3753 T4705 T4703 pobj plates,in
R3754 T4706 T4705 acl containing,plates
R3755 T4707 T4706 dobj PBS,containing
R3756 T4708 T4702 punct .,contained
R3757 T4709 T4724 prep For,used
R3758 T4710 T4711 amod stereological,study
R3759 T4711 T4709 pobj study,For
R3760 T4712 T4724 punct ",",used
R3761 T4713 T4715 det every,section
R3762 T4714 T4715 amod sixth,section
R3763 T4715 T4724 nsubjpass section,used
R3764 T4716 T4715 prep in,section
R3765 T4717 T4718 det the,series
R3766 T4718 T4716 pobj series,in
R3767 T4719 T4715 prep throughout,section
R3768 T4720 T4722 det the,hippocampus
R3769 T4721 T4722 amod entire,hippocampus
R3770 T4722 T4719 pobj hippocampus,throughout
R3771 T4723 T4724 auxpass was,used
R3772 T4724 T4724 ROOT used,used
R3773 T4725 T4724 prep in,used
R3774 T4726 T4727 det some,animals
R3775 T4727 T4725 pobj animals,in
R3776 T4728 T4724 punct .,used