PMC:3291650 / 43336-44296 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3291650","sourcedb":"PMC","sourceid":"3291650","source_url":"http://www.ncbi.nlm.nih.gov/pmc/3291650","text":"Adaptive immunity is not altered by the absence of A20 in myeloid cells following lethal IAV infection. Wild type (A20myel-WT) and A20myel-KO mice were infected intranasally with a lethal dose of IAV. 6 days p.i. mice were sacrificed and analyzed. (A) TNF and IL-1β protein levels in BAL fluid were measured by ELISA and multiplexing technologies. (B) Viral titers in the lung were measured and expressed as mean TCID50. The dashed line represents the detection limit. (C) Total CD8+, GrB and IFNγ expressing T cells in BAL fluid were measured using flow cytometry. (D) IFNγ protein levels in BAL fluid were measured by ELISA. (E) Total CD8+, GrB and IFNγ expressing T cells in lung parenchyma were measured using flow cytometry. (F) Virus specific antibody titers in serum were determined via a hemagglutination inhibition (HAI) assay. The dashed line depicts the detection limit. Numbers are averages +/− SEM of at least 5 mice per group. *p\u003c0.05; **p\u003c0.01.\n","tracks":[{"project":"sentences","denotations":[{"id":"T298","span":{"begin":104,"end":200},"obj":"Sentence"},{"id":"T299","span":{"begin":201,"end":420},"obj":"Sentence"},{"id":"T300","span":{"begin":421,"end":836},"obj":"Sentence"},{"id":"T301","span":{"begin":837,"end":881},"obj":"Sentence"},{"id":"T302","span":{"begin":882,"end":959},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T298","pred":"source","obj":"sentences"},{"subj":"T299","pred":"source","obj":"sentences"},{"subj":"T300","pred":"source","obj":"sentences"},{"subj":"T301","pred":"source","obj":"sentences"},{"subj":"T302","pred":"source","obj":"sentences"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#e093ec","default":true}]}]}}