PMC:3291650 / 42465-43282 JSONTXT

{"project":"sentences","denotations":[{"id":"T289","span":{"begin":107,"end":206},"obj":"Sentence"},{"id":"T290","span":{"begin":207,"end":391},"obj":"Sentence"},{"id":"T291","span":{"begin":392,"end":515},"obj":"Sentence"},{"id":"T292","span":{"begin":516,"end":643},"obj":"Sentence"},{"id":"T293","span":{"begin":644,"end":705},"obj":"Sentence"},{"id":"T294","span":{"begin":706,"end":816},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Adaptive immunity is not altered by the absence of A20 in myeloid cells following sublethal IAV infection. Wild type (A20myel-WT) and A20myel-KO mice were infected intranasally with a sublethal dose of IAV. BAL was isolated from IAV infected mice at 4, 7 and 10 days p.i. and analyzed for total CD8+ T cells, Granzyme B (GrB) and IFNγ expressing CD8+ T cells (A) and IFNγ protein levels (B). Total CD8+ T cells, GrB and IFNγ expressing CD8+ T cells in the lung parenchyma were also determined by flow cytometry (C). Virus specific antibody titers in serum at 4, 7 and 10 days p.i. were determined via a hemagglutination inhibition (HAI) assay. The dashed line depicts the detection limit of the assay (D). Numbers are averages +/− SEM of at least 5 mice per group and are representative of 2 independent experiments.\n"}