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PMC:3291650 / 41814-44338 JSONTXT

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Id Subject Object Predicate Lexical cue
T279 0-22 Sentence denotes Supporting Information
T280 23-106 Sentence denotes Figure S1 A20 deficient alveolar macrophages are hyperresponsive to IAV infection.
T281 107-245 Sentence denotes Alveolar macrophages isolated from A20myel-KO and wild type (A20myel-WT) mice were mock treated or infected with IAV (moi 1) for 18 hours.
T282 246-329 Sentence denotes TNF, MCP-1 (ccl2), KC (cxcl1) and IFNα4 mRNA expression was determined by qPCR (A).
T283 330-448 Sentence denotes Cell culture supernatant was analyzed for TNF, MCP-1 and KC protein levels by ELISA and multiplexing technologies (B).
T284 449-507 Sentence denotes Error bars represent mean values (+/− SEM) of 2–3 samples.
T285 508-596 Sentence denotes Results are representative for 2 independent experiments. *p<0.05; **p<0.01; ***p<0.001.
T286 597-602 Sentence denotes (TIF)
T287 603-639 Sentence denotes Click here for additional data file.
T288 640-757 Sentence denotes Figure S2 Adaptive immunity is not altered by the absence of A20 in myeloid cells following sublethal IAV infection.
T289 758-857 Sentence denotes Wild type (A20myel-WT) and A20myel-KO mice were infected intranasally with a sublethal dose of IAV.
T290 858-1042 Sentence denotes BAL was isolated from IAV infected mice at 4, 7 and 10 days p.i. and analyzed for total CD8+ T cells, Granzyme B (GrB) and IFNγ expressing CD8+ T cells (A) and IFNγ protein levels (B).
T291 1043-1166 Sentence denotes Total CD8+ T cells, GrB and IFNγ expressing CD8+ T cells in the lung parenchyma were also determined by flow cytometry (C).
T292 1167-1294 Sentence denotes Virus specific antibody titers in serum at 4, 7 and 10 days p.i. were determined via a hemagglutination inhibition (HAI) assay.
T293 1295-1356 Sentence denotes The dashed line depicts the detection limit of the assay (D).
T294 1357-1467 Sentence denotes Numbers are averages +/− SEM of at least 5 mice per group and are representative of 2 independent experiments.
T295 1468-1473 Sentence denotes (TIF)
T296 1474-1510 Sentence denotes Click here for additional data file.
T297 1511-1625 Sentence denotes Figure S3 Adaptive immunity is not altered by the absence of A20 in myeloid cells following lethal IAV infection.
T298 1626-1722 Sentence denotes Wild type (A20myel-WT) and A20myel-KO mice were infected intranasally with a lethal dose of IAV.
T299 1723-1942 Sentence denotes 6 days p.i. mice were sacrificed and analyzed. (A) TNF and IL-1β protein levels in BAL fluid were measured by ELISA and multiplexing technologies. (B) Viral titers in the lung were measured and expressed as mean TCID50.
T300 1943-2358 Sentence denotes The dashed line represents the detection limit. (C) Total CD8+, GrB and IFNγ expressing T cells in BAL fluid were measured using flow cytometry. (D) IFNγ protein levels in BAL fluid were measured by ELISA. (E) Total CD8+, GrB and IFNγ expressing T cells in lung parenchyma were measured using flow cytometry. (F) Virus specific antibody titers in serum were determined via a hemagglutination inhibition (HAI) assay.
T301 2359-2403 Sentence denotes The dashed line depicts the detection limit.
T302 2404-2481 Sentence denotes Numbers are averages +/− SEM of at least 5 mice per group. *p<0.05; **p<0.01.
T303 2482-2487 Sentence denotes (TIF)
T304 2488-2524 Sentence denotes Click here for additional data file.