PMC:3291650 / 38831-39727 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3291650","sourcedb":"PMC","sourceid":"3291650","source_url":"http://www.ncbi.nlm.nih.gov/pmc/3291650","text":"Flow cytometry\nLungs were dissected and incubated with collagenase type IV (1 mg/ml; Sigma) and DNAse (100 U/ml; Roche) at 37°C for 30 min. Subsequently, samples were filtered through a 70 µm and 40 µm nylon mesh. For the preparation of BAL, trachea were canulated and airway lumen was flushed 4 times with HBSS with 1 mM EDTA. Cells were stained with monoclonal antibodies directed against MHC-II (I-A/I-E) FITC (M5/114.15.2), CD11c PerCP-Cy5.5 (N418), F4/80 APC (BM8), CD62L PE (MEL-14), Granzyme B FITC (NGZB) from eBiosciences and CD3 Molecular Complex Horizon v450 (17A2), Ly6C Horizon v450 (AL-21), Ly6G PE (1A8), CD11b APC-Cy7 (M1/70), CD8α PerCP (53-6.7), IFNγ Alexa 647 (XMG1.2) and CD16/32 (2.4G2) from BD Pharmingen. 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