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PMC:3291650 / 37713-38058 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T17294 341-344 NN denotes min
T17293 339-340 CD denotes 5
T17292 335-338 IN denotes for
T17291 333-334 NN denotes g
T17290 329-332 CD denotes 500
T17289 326-328 IN denotes at
T17288 314-325 VB denotes centrifuged
T17287 309-313 VB denotes were
T17286 303-308 NN denotes cells
T17285 299-302 CC denotes and
T17284 297-298 NN denotes %
T17283 293-297 CD denotes 0.65
T17282 290-292 IN denotes of
T17281 276-289 NN denotes concentration
T17280 270-275 JJ denotes final
T17279 268-269 DT denotes a
T17278 265-267 TO denotes to
T17277 259-264 VB denotes added
T17276 255-258 VB denotes was
T17275 245-254 NN denotes detergent
T17274 239-244 NN denotes NP-40
T17273 237-238 -COMMA- denotes ,
T17272 233-237 NNP denotes Next
T17271 228-232 NNP denotes 4°C.
T17270 225-227 IN denotes at
T17269 221-224 NN denotes min
T17268 218-220 CD denotes 15
T17267 214-217 IN denotes for
T17266 212-213 -RRB- denotes )
T17265 202-212 NN denotes inhibitors
T17264 190-201 NN denotes phosphatase
T17263 186-189 CC denotes and
T17262 177-185 NN denotes protease
T17261 172-176 IN denotes with
T17260 159-171 VB denotes supplemented
T17259 154-158 NN denotes EGTA
T17258 151-153 NN denotes mM
T17257 147-150 CD denotes 0.1
T17256 143-146 CC denotes and
T17255 138-142 NN denotes EDTA
T17254 135-137 NN denotes mM
T17253 131-134 CD denotes 0.5
T17252 129-130 -COMMA- denotes ,
T17251 121-129 NN denotes glycerol
T17250 119-120 NN denotes %
T17249 118-119 CD denotes 5
T17248 116-117 -COMMA- denotes ,
T17247 111-116 NN denotes MgCl2
T17246 108-110 NN denotes mM
T17245 106-107 CD denotes 1
T17244 104-105 -COMMA- denotes ,
T17243 101-104 NN denotes KCl
T17242 98-100 NN denotes mM
T17241 95-97 CD denotes 10
T17240 93-94 -COMMA- denotes ,
T17239 90-93 CD denotes 7.5
T17238 87-89 NN denotes pH
T17237 81-86 NNP denotes Hepes
T17236 78-80 NN denotes mM
T17235 75-77 CD denotes 10
T17234 74-75 -LRB- denotes (
T17233 71-73 NN denotes B1
T17232 68-70 IN denotes in
T17231 62-67 NN denotes cells
T17230 49-61 VB denotes resuspending
T17229 46-48 IN denotes by
T17228 37-45 VB denotes prepared
T17227 32-36 VB denotes were
T17226 24-31 NN denotes lysates
T17225 12-23 JJ denotes cytoplasmic
T17224 8-11 CC denotes and
T17223 0-7 JJ denotes Nuclear
R12994 T17223 T17224 arg1Of Nuclear,and
R12995 T17225 T17224 arg2Of cytoplasmic,and
R12996 T17226 T17223 arg1Of lysates,Nuclear
R12997 T17226 T17225 arg1Of lysates,cytoplasmic
R12998 T17226 T17227 arg1Of lysates,were
R12999 T17226 T17228 arg2Of lysates,prepared
R13000 T17228 T17227 arg2Of prepared,were
R13001 T17228 T17229 arg1Of prepared,by
R13002 T17228 T17273 arg1Of prepared,","
R13003 T17230 T17229 arg2Of resuspending,by
R13004 T17230 T17232 arg1Of resuspending,in
R13005 T17230 T17267 arg1Of resuspending,for
R13006 T17231 T17230 arg2Of cells,resuspending
R13007 T17233 T17232 arg2Of B1,in
R13008 T17233 T17234 arg1Of B1,(
R13009 T17237 T17235 arg1Of Hepes,10
R13010 T17237 T17236 arg1Of Hepes,mM
R13011 T17238 T17239 arg1Of pH,7.5
R13012 T17238 T17240 arg1Of pH,","
R13013 T17240 T17244 arg1Of ",",","
R13014 T17241 T17242 arg1Of 10,mM
R13015 T17243 T17240 arg2Of KCl,","
R13016 T17243 T17241 arg1Of KCl,10
R13017 T17244 T17248 arg1Of ",",","
R13018 T17245 T17246 arg1Of 1,mM
R13019 T17247 T17244 arg2Of MgCl2,","
R13020 T17247 T17245 arg1Of MgCl2,1
R13021 T17248 T17237 arg1Of ",",Hepes
R13022 T17248 T17252 arg1Of ",",","
R13023 T17249 T17250 arg1Of 5,%
R13024 T17251 T17248 arg2Of glycerol,","
R13025 T17251 T17249 arg1Of glycerol,5
R13026 T17252 T17256 arg1Of ",",and
R13027 T17253 T17254 arg1Of 0.5,mM
R13028 T17255 T17252 arg2Of EDTA,","
R13029 T17255 T17253 arg1Of EDTA,0.5
R13030 T17256 T17234 arg2Of and,(
R13031 T17256 T17260 arg2Of and,supplemented
R13032 T17257 T17258 arg1Of 0.1,mM
R13033 T17259 T17256 arg2Of EGTA,and
R13034 T17259 T17257 arg1Of EGTA,0.1
R13035 T17260 T17261 arg1Of supplemented,with
R13036 T17262 T17263 arg1Of protease,and
R13037 T17264 T17263 arg2Of phosphatase,and
R13038 T17265 T17261 arg2Of inhibitors,with
R13039 T17265 T17262 arg1Of inhibitors,protease
R13040 T17265 T17264 arg1Of inhibitors,phosphatase
R13041 T17266 T17234 arg3Of ),(
R13042 T17269 T17267 arg2Of min,for
R13043 T17269 T17268 arg1Of min,15
R13044 T17269 T17270 arg1Of min,at
R13045 T17272 T17270 arg2Of Next,at
R13046 T17272 T17271 arg1Of Next,4°C.
R13047 T17275 T17274 arg1Of detergent,NP-40
R13048 T17275 T17276 arg1Of detergent,was
R13049 T17275 T17277 arg2Of detergent,added
R13050 T17277 T17276 arg2Of added,was
R13051 T17277 T17278 arg1Of added,to
R13052 T17277 T17285 arg1Of added,and
R13053 T17281 T17278 arg2Of concentration,to
R13054 T17281 T17279 arg1Of concentration,a
R13055 T17281 T17280 arg1Of concentration,final
R13056 T17281 T17282 arg1Of concentration,of
R13057 T17284 T17282 arg2Of %,of
R13058 T17284 T17283 arg1Of %,0.65
R13059 T17285 T17273 arg2Of and,","
R13060 T17286 T17287 arg1Of cells,were
R13061 T17286 T17288 arg2Of cells,centrifuged
R13062 T17288 T17285 arg2Of centrifuged,and
R13063 T17288 T17287 arg2Of centrifuged,were
R13064 T17288 T17289 arg1Of centrifuged,at
R13065 T17288 T17292 arg1Of centrifuged,for
R13066 T17291 T17289 arg2Of g,at
R13067 T17291 T17290 arg1Of g,500
R13068 T17294 T17292 arg2Of min,for
R13069 T17294 T17293 arg1Of min,5

GO-BP

Id Subject Object Predicate Lexical cue
T17169 190-201 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase

GO-MF

Id Subject Object Predicate Lexical cue
T17172 190-201 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase

GO-CC

Id Subject Object Predicate Lexical cue
T17176 303-308 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T17175 62-67 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

sentences

Id Subject Object Predicate Lexical cue
T17162 0-345 Sentence denotes Nuclear and cytoplasmic lysates were prepared by resuspending cells in B1 (10 mM Hepes pH 7.5, 10 mM KCl, 1 mM MgCl2, 5% glycerol, 0.5 mM EDTA and 0.1 mM EGTA supplemented with protease and phosphatase inhibitors) for 15 min at 4°C. Next, NP-40 detergent was added to a final concentration of 0.65% and cells were centrifuged at 500 g for 5 min.
T242 0-232 Sentence denotes Nuclear and cytoplasmic lysates were prepared by resuspending cells in B1 (10 mM Hepes pH 7.5, 10 mM KCl, 1 mM MgCl2, 5% glycerol, 0.5 mM EDTA and 0.1 mM EGTA supplemented with protease and phosphatase inhibitors) for 15 min at 4°C.
T243 233-345 Sentence denotes Next, NP-40 detergent was added to a final concentration of 0.65% and cells were centrifuged at 500 g for 5 min.

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T17788 118-129 Entity denotes 5% glycerol
T17778 177-185 Protein denotes protease
T17776 131-142 Entity denotes 0.5 mM EDTA
T17775 303-308 Entity denotes cells
T17772 190-201 Protein denotes phosphatase
T17771 75-89 Entity denotes 10 mM Hepes pH
T17768 245-254 Entity denotes detergent
T17765 190-212 Entity denotes phosphatase inhibitors
T17764 46-67 Entity denotes by resuspending cells
T17761 101-104 Entity denotes KCl
T17759 71-73 Entity denotes B1
T17756 81-86 Entity denotes Hepes
T17754 147-158 Entity denotes 0.1 mM EGTA

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T17565 344-345 . denotes .
T17564 341-344 NN denotes min
T17563 339-340 CD denotes 5
T17562 335-338 IN denotes for
T17561 333-334 NN denotes g
T17560 329-332 CD denotes 500
T17559 326-328 IN denotes at
T17558 314-325 VBN denotes centrifuged
T17557 309-313 VBD denotes were
T17556 303-308 NNS denotes cells
T17555 299-302 CC denotes and
T17554 297-298 NN denotes %
T17553 293-297 CD denotes 0.65
T17552 290-292 IN denotes of
T17551 276-289 NN denotes concentration
T17550 270-275 JJ denotes final
T17549 268-269 DT denotes a
T17548 265-267 TO denotes to
T17547 259-264 VBN denotes added
T17546 255-258 VBD denotes was
T17545 245-254 NN denotes detergent
T17544 239-244 NNP denotes NP-40
T17543 237-238 , denotes ,
T17542 233-237 NNP denotes Next
T17541 230-232 NNP denotes C.
T17540 229-230 CD denotes °
T17539 228-229 CD denotes 4
T17538 225-227 IN denotes at
T17537 221-224 NN denotes min
T17536 218-220 CD denotes 15
T17535 214-217 IN denotes for
T17534 212-213 -RRB- denotes )
T17533 202-212 NNS denotes inhibitors
T17532 190-201 NN denotes phosphatase
T17531 186-189 CC denotes and
T17530 177-185 NN denotes protease
T17529 172-176 IN denotes with
T17528 159-171 VBD denotes supplemented
T17527 154-158 NNP denotes EGTA
T17526 151-153 NNP denotes mM
T17525 147-150 CD denotes 0.1
T17524 143-146 CC denotes and
T17523 138-142 NNP denotes EDTA
T17522 135-137 NNP denotes mM
T17521 131-134 CD denotes 0.5
T17520 129-130 , denotes ,
T17519 121-129 NN denotes glycerol
T17518 119-120 NN denotes %
T17517 118-119 CD denotes 5
T17516 116-117 , denotes ,
T17515 111-116 NNP denotes MgCl2
T17514 108-110 NNP denotes mM
T17513 106-107 CD denotes 1
T17512 104-105 , denotes ,
T17511 101-104 NNP denotes KCl
T17510 98-100 NNP denotes mM
T17509 95-97 CD denotes 10
T17508 93-94 , denotes ,
T17507 90-93 CD denotes 7.5
T17506 87-89 NNP denotes pH
T17505 81-86 NNP denotes Hepes
T17504 78-80 NNP denotes mM
T17503 75-77 CD denotes 10
T17502 74-75 -LRB- denotes (
T17501 71-73 NNP denotes B1
T17500 68-70 IN denotes in
T17499 62-67 NNS denotes cells
T17498 49-61 VBG denotes resuspending
T17497 46-48 IN denotes by
T17496 37-45 VBN denotes prepared
T17495 32-36 VBD denotes were
T17494 24-31 NNS denotes lysates
T17493 12-23 JJ denotes cytoplasmic
T17492 8-11 CC denotes and
T17491 0-7 NNP denotes Nuclear
R13275 T17491 T17494 amod Nuclear,lysates
R13276 T17492 T17491 cc and,Nuclear
R13277 T17493 T17491 conj cytoplasmic,Nuclear
R13278 T17494 T17496 nsubjpass lysates,prepared
R13279 T17495 T17496 auxpass were,prepared
R13280 T17496 T17496 ROOT prepared,prepared
R13281 T17497 T17496 agent by,prepared
R13282 T17498 T17497 pcomp resuspending,by
R13283 T17499 T17498 dobj cells,resuspending
R13284 T17500 T17498 prep in,resuspending
R13285 T17501 T17500 pobj B1,in
R13286 T17502 T17501 punct (,B1
R13287 T17503 T17506 nummod 10,pH
R13288 T17504 T17506 compound mM,pH
R13289 T17505 T17506 compound Hepes,pH
R13290 T17506 T17501 appos pH,B1
R13291 T17507 T17511 nummod 7.5,KCl
R13292 T17508 T17511 punct ",",KCl
R13293 T17509 T17511 nummod 10,KCl
R13294 T17510 T17511 compound mM,KCl
R13295 T17511 T17501 appos KCl,B1
R13296 T17512 T17511 punct ",",KCl
R13297 T17513 T17515 nummod 1,MgCl2
R13298 T17514 T17515 compound mM,MgCl2
R13299 T17515 T17511 conj MgCl2,KCl
R13300 T17516 T17515 punct ",",MgCl2
R13301 T17517 T17518 nummod 5,%
R13302 T17518 T17519 compound %,glycerol
R13303 T17519 T17515 conj glycerol,MgCl2
R13304 T17520 T17519 punct ",",glycerol
R13305 T17521 T17523 nummod 0.5,EDTA
R13306 T17522 T17523 compound mM,EDTA
R13307 T17523 T17519 appos EDTA,glycerol
R13308 T17524 T17519 cc and,glycerol
R13309 T17525 T17527 nummod 0.1,EGTA
R13310 T17526 T17527 compound mM,EGTA
R13311 T17527 T17528 nsubj EGTA,supplemented
R13312 T17528 T17496 advcl supplemented,prepared
R13313 T17529 T17528 prep with,supplemented
R13314 T17530 T17529 pobj protease,with
R13315 T17531 T17530 cc and,protease
R13316 T17532 T17533 compound phosphatase,inhibitors
R13317 T17533 T17530 conj inhibitors,protease
R13318 T17534 T17528 punct ),supplemented
R13319 T17535 T17528 prep for,supplemented
R13320 T17536 T17537 nummod 15,min
R13321 T17537 T17535 pobj min,for
R13322 T17538 T17528 prep at,supplemented
R13323 T17539 T17540 nummod 4,°
R13324 T17540 T17542 nummod °,Next
R13325 T17541 T17542 compound C.,Next
R13326 T17542 T17538 pobj Next,at
R13327 T17543 T17547 punct ",",added
R13328 T17544 T17545 compound NP-40,detergent
R13329 T17545 T17547 nsubjpass detergent,added
R13330 T17546 T17547 auxpass was,added
R13331 T17547 T17496 conj added,prepared
R13332 T17548 T17547 prep to,added
R13333 T17549 T17551 det a,concentration
R13334 T17550 T17551 amod final,concentration
R13335 T17551 T17548 pobj concentration,to
R13336 T17552 T17551 prep of,concentration
R13337 T17553 T17554 nummod 0.65,%
R13338 T17554 T17552 pobj %,of
R13339 T17555 T17551 cc and,concentration
R13340 T17556 T17551 conj cells,concentration
R13341 T17557 T17558 auxpass were,centrifuged
R13342 T17558 T17547 conj centrifuged,added
R13343 T17559 T17558 prep at,centrifuged
R13344 T17560 T17561 nummod 500,g
R13345 T17561 T17559 pobj g,at
R13346 T17562 T17558 prep for,centrifuged
R13347 T17563 T17564 nummod 5,min
R13348 T17564 T17562 pobj min,for
R13349 T17565 T17547 punct .,added

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T17726 151-201 Protein denotes mM EGTA supplemented with protease and phosphatase