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have isolated 1,264 olfactory receptor cDNA clones, which together confirm the olfactory epithelial expression of 419 annotated olfactory receptor genes. We used low-stringency hybridization with degenerate olfactory receptor DNA probes to screen around 4,100,000 plaque-forming units (pfu) of an adult mouse olfactory epithelium cDNA library and around 640,000 pfu of an embryonic olfactory epithelium library. We obtained sequences from 1,715 hybridization-positive cDNAs following secondary screens to isolate single clones. Of these clones, 1,264 yielded olfactory receptor-containing sequences. The 26% false-positive rate is a consequence of using low-stringency hybridization to obtain maximal sensitivity. Continuing the screen would have resulted in cDNAs from additional olfactory receptors, but we reached a point of limiting returns: our final batch of 45 olfactory receptor-positive sequences represented 33 different olfactory receptors, of which only four had not been encountered previously in our screen."}

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{"project":"craft-ca-core-dev","denotations":[{"id":"T2430","span":{"begin":23,"end":32},"obj":"GO:0007608"},{"id":"T2431","span":{"begin":42,"end":53},"obj":"SO:0000317"},{"id":"T2432","span":{"begin":82,"end":91},"obj":"GO:0007608"},{"id":"T2433","span":{"begin":82,"end":102},"obj":"UBERON:0001997"},{"id":"T2434","span":{"begin":103,"end":113},"obj":"GO:0010467"},{"id":"T2435","span":{"begin":131,"end":140},"obj":"GO:0007608"},{"id":"T2436","span":{"begin":150,"end":155},"obj":"SO:0000704"},{"id":"T2437","span":{"begin":180,"end":193},"obj":"GO:0097617"},{"id":"T2438","span":{"begin":210,"end":219},"obj":"GO:0007608"},{"id":"T2439","span":{"begin":300,"end":305},"obj":"UBERON:0007023"},{"id":"T2440","span":{"begin":306,"end":311},"obj":"NCBITaxon:10088"},{"id":"T2441","span":{"begin":312,"end":321},"obj":"GO:0007608"},{"id":"T2442","span":{"begin":312,"end":332},"obj":"UBERON:0001997"},{"id":"T2443","span":{"begin":375,"end":384},"obj":"UBERON:0000922"},{"id":"T2444","span":{"begin":385,"end":394},"obj":"GO:0007608"},{"id":"T2445","span":{"begin":385,"end":405},"obj":"UBERON:0001997"},{"id":"T2446","span":{"begin":448,"end":461},"obj":"GO:0097617"},{"id":"T2447","span":{"begin":562,"end":571},"obj":"GO:0007608"},{"id":"T2448","span":{"begin":672,"end":685},"obj":"GO:0097617"},{"id":"T2449","span":{"begin":784,"end":793},"obj":"GO:0007608"},{"id":"T2450","span":{"begin":871,"end":880},"obj":"GO:0007608"},{"id":"T2451","span":{"begin":934,"end":943},"obj":"GO:0007608"}],"text":"We have isolated 1,264 olfactory receptor cDNA clones, which together confirm the olfactory epithelial expression of 419 annotated olfactory receptor genes. We used low-stringency hybridization with degenerate olfactory receptor DNA probes to screen around 4,100,000 plaque-forming units (pfu) of an adult mouse olfactory epithelium cDNA library and around 640,000 pfu of an embryonic olfactory epithelium library. We obtained sequences from 1,715 hybridization-positive cDNAs following secondary screens to isolate single clones. Of these clones, 1,264 yielded olfactory receptor-containing sequences. The 26% false-positive rate is a consequence of using low-stringency hybridization to obtain maximal sensitivity. Continuing the screen would have resulted in cDNAs from additional olfactory receptors, but we reached a point of limiting returns: our final batch of 45 olfactory receptor-positive sequences represented 33 different olfactory receptors, of which only four had not been encountered previously in our screen."}