Id |
Subject |
Object |
Predicate |
Lexical cue |
T12273 |
0-3 |
DT |
denotes |
The |
T12274 |
14-23 |
NNS |
denotes |
epithelia |
T12275 |
4-13 |
JJ |
denotes |
olfactory |
T12276 |
29-38 |
VBN |
denotes |
dissected |
T12277 |
24-28 |
VBD |
denotes |
were |
T12278 |
39-43 |
IN |
denotes |
from |
T12279 |
44-49 |
CD |
denotes |
three |
T12280 |
71-75 |
NNS |
denotes |
mice |
T12281 |
50-55 |
JJ |
denotes |
adult |
T12282 |
56-62 |
JJ |
denotes |
female |
T12283 |
63-68 |
NN |
denotes |
C57BL |
T12284 |
68-69 |
HYPH |
denotes |
/ |
T12285 |
69-70 |
CD |
denotes |
6 |
T12286 |
75-77 |
, |
denotes |
, |
T12287 |
77-86 |
VBG |
denotes |
including |
T12288 |
87-94 |
NNS |
denotes |
tissues |
T12289 |
95-103 |
VBN |
denotes |
attached |
T12290 |
104-106 |
IN |
denotes |
to |
T12291 |
107-110 |
DT |
denotes |
the |
T12292 |
111-116 |
NN |
denotes |
skull |
T12293 |
117-120 |
CC |
denotes |
and |
T12294 |
121-127 |
NN |
denotes |
septum |
T12295 |
127-128 |
. |
denotes |
. |
T12296 |
128-241 |
sentence |
denotes |
RNA was isolated using the Qiagen RNeasy midi kit (Qiagen, Valencia, CA, USA), including a DNase treatment step. |
T12297 |
129-132 |
NN |
denotes |
RNA |
T12298 |
137-145 |
VBN |
denotes |
isolated |
T12299 |
133-136 |
VBD |
denotes |
was |
T12300 |
146-151 |
VBG |
denotes |
using |
T12301 |
152-155 |
DT |
denotes |
the |
T12302 |
175-178 |
NN |
denotes |
kit |
T12303 |
156-162 |
NNP |
denotes |
Qiagen |
T12304 |
163-169 |
NNP |
denotes |
RNeasy |
T12305 |
170-174 |
NN |
denotes |
midi |
T12306 |
179-180 |
-LRB- |
denotes |
( |
T12307 |
180-186 |
NNP |
denotes |
Qiagen |
T12308 |
186-188 |
, |
denotes |
, |
T12309 |
188-196 |
NNP |
denotes |
Valencia |
T12310 |
196-198 |
, |
denotes |
, |
T12311 |
198-200 |
NNP |
denotes |
CA |
T12312 |
200-202 |
, |
denotes |
, |
T12313 |
202-205 |
NNP |
denotes |
USA |
T12314 |
205-206 |
-RRB- |
denotes |
) |
T12315 |
206-208 |
, |
denotes |
, |
T12316 |
208-217 |
VBG |
denotes |
including |
T12317 |
218-219 |
DT |
denotes |
a |
T12318 |
236-240 |
NN |
denotes |
step |
T12319 |
220-225 |
NN |
denotes |
DNase |
T12320 |
226-235 |
NN |
denotes |
treatment |
T12321 |
240-241 |
. |
denotes |
. |
T12322 |
241-470 |
sentence |
denotes |
First-strand cDNA was produced from 2.5 μg of RNA in a volume of 50 μl using random hexamers and Invitrogen's Superscript II reverse transcriptase (Invitrogen, Carlsbad, CA, USA), according to the manufacturer's recommendations. |
T12323 |
242-247 |
JJ |
denotes |
First |
T12324 |
248-254 |
NN |
denotes |
strand |
T12325 |
247-248 |
HYPH |
denotes |
- |
T12326 |
255-259 |
NN |
denotes |
cDNA |
T12327 |
264-272 |
VBN |
denotes |
produced |
T12328 |
260-263 |
VBD |
denotes |
was |
T12329 |
273-277 |
IN |
denotes |
from |
T12330 |
278-281 |
CD |
denotes |
2.5 |
T12331 |
282-284 |
NNS |
denotes |
μg |
T12332 |
285-287 |
IN |
denotes |
of |
T12333 |
288-291 |
NN |
denotes |
RNA |
T12334 |
292-294 |
IN |
denotes |
in |
T12335 |
295-296 |
DT |
denotes |
a |
T12336 |
297-303 |
NN |
denotes |
volume |
T12337 |
304-306 |
IN |
denotes |
of |
T12338 |
307-309 |
CD |
denotes |
50 |
T12339 |
310-312 |
NNS |
denotes |
μl |
T12340 |
313-318 |
VBG |
denotes |
using |
T12341 |
319-325 |
JJ |
denotes |
random |
T12342 |
326-334 |
NNS |
denotes |
hexamers |
T12343 |
335-338 |
CC |
denotes |
and |
T12344 |
339-349 |
NNP |
denotes |
Invitrogen |
T12345 |
375-388 |
NN |
denotes |
transcriptase |
T12346 |
349-351 |
POS |
denotes |
's |
T12347 |
352-363 |
NN |
denotes |
Superscript |
T12348 |
364-366 |
CD |
denotes |
II |
T12349 |
367-374 |
JJ |
denotes |
reverse |
T12350 |
389-390 |
-LRB- |
denotes |
( |
T12351 |
390-400 |
NNP |
denotes |
Invitrogen |
T12352 |
400-402 |
, |
denotes |
, |
T12353 |
402-410 |
NNP |
denotes |
Carlsbad |
T12354 |
410-412 |
, |
denotes |
, |
T12355 |
412-414 |
NNP |
denotes |
CA |
T12356 |
414-416 |
, |
denotes |
, |
T12357 |
416-419 |
NNP |
denotes |
USA |
T12358 |
419-420 |
-RRB- |
denotes |
) |
T12359 |
420-422 |
, |
denotes |
, |
T12360 |
422-431 |
VBG |
denotes |
according |
T12361 |
432-434 |
IN |
denotes |
to |
T12362 |
435-438 |
DT |
denotes |
the |
T12363 |
439-451 |
NN |
denotes |
manufacturer |
T12364 |
454-469 |
NNS |
denotes |
recommendations |
T12365 |
451-453 |
POS |
denotes |
's |
T12366 |
469-470 |
. |
denotes |
. |
T12367 |
470-559 |
sentence |
denotes |
One-twenty-fifth of the resulting cDNA was used as template in subsequent PCR reactions. |
T12368 |
471-474 |
CD |
denotes |
One |
T12369 |
482-487 |
NN |
denotes |
fifth |
T12370 |
474-475 |
HYPH |
denotes |
- |
T12371 |
475-481 |
CD |
denotes |
twenty |
T12372 |
481-482 |
HYPH |
denotes |
- |
T12373 |
514-518 |
VBN |
denotes |
used |
T12374 |
488-490 |
IN |
denotes |
of |
T12375 |
491-494 |
DT |
denotes |
the |
T12376 |
505-509 |
NN |
denotes |
cDNA |
T12377 |
495-504 |
VBG |
denotes |
resulting |
T12378 |
510-513 |
VBD |
denotes |
was |
T12379 |
519-521 |
IN |
denotes |
as |
T12380 |
522-530 |
NN |
denotes |
template |
T12381 |
531-533 |
IN |
denotes |
in |
T12382 |
534-544 |
JJ |
denotes |
subsequent |
T12383 |
549-558 |
NNS |
denotes |
reactions |
T12384 |
545-548 |
NN |
denotes |
PCR |
T12385 |
558-559 |
. |
denotes |
. |
T12386 |
559-747 |
sentence |
denotes |
PCR amplification biased towards class I olfactory receptors was performed using degenerate primers P26 [17] and classI_R1 (5'-GGRTTIADIRYIGGNGG-3') with an annealing temperature of 44°C. |
T12387 |
560-563 |
NN |
denotes |
PCR |
T12388 |
564-577 |
NN |
denotes |
amplification |
T12389 |
625-634 |
VBN |
denotes |
performed |
T12390 |
578-584 |
JJ |
denotes |
biased |
T12391 |
585-592 |
IN |
denotes |
towards |
T12392 |
593-598 |
NN |
denotes |
class |
T12393 |
611-620 |
NNS |
denotes |
receptors |
T12394 |
599-600 |
CD |
denotes |
I |
T12395 |
601-610 |
JJ |
denotes |
olfactory |
T12396 |
621-624 |
VBD |
denotes |
was |
T12397 |
635-640 |
VBG |
denotes |
using |
T12398 |
641-651 |
JJ |
denotes |
degenerate |
T12399 |
660-663 |
NN |
denotes |
P26 |
T12400 |
652-659 |
NNS |
denotes |
primers |
T12401 |
664-665 |
-LRB- |
denotes |
[ |
T12402 |
665-667 |
CD |
denotes |
17 |
T12403 |
667-668 |
-RRB- |
denotes |
] |
T12404 |
669-672 |
CC |
denotes |
and |
T12405 |
673-682 |
NN |
denotes |
classI_R1 |
T12406 |
683-684 |
-LRB- |
denotes |
( |
T12407 |
687-704 |
NN |
denotes |
GGRTTIADIRYIGGNGG |
T12408 |
684-685 |
CD |
denotes |
5 |
T12409 |
685-686 |
SYM |
denotes |
' |
T12410 |
686-687 |
HYPH |
denotes |
- |
T12411 |
704-705 |
HYPH |
denotes |
- |
T12412 |
705-706 |
CD |
denotes |
3 |
T12413 |
706-707 |
SYM |
denotes |
' |
T12414 |
707-708 |
-RRB- |
denotes |
) |
T12415 |
709-713 |
IN |
denotes |
with |
T12416 |
714-716 |
DT |
denotes |
an |
T12417 |
727-738 |
NN |
denotes |
temperature |
T12418 |
717-726 |
JJ |
denotes |
annealing |
T12419 |
739-741 |
IN |
denotes |
of |
T12420 |
742-744 |
CD |
denotes |
44 |
T12421 |
744-746 |
NNS |
denotes |
°C |
T12422 |
746-747 |
. |
denotes |
. |
T12423 |
747-838 |
sentence |
denotes |
The product was cloned (TA cloning kit, Invitrogen), and individual clones were sequenced. |
T12424 |
748-751 |
DT |
denotes |
The |
T12425 |
752-759 |
NN |
denotes |
product |
T12426 |
764-770 |
VBN |
denotes |
cloned |
T12427 |
760-763 |
VBD |
denotes |
was |
T12428 |
771-772 |
-LRB- |
denotes |
( |
T12429 |
788-798 |
NNP |
denotes |
Invitrogen |
T12430 |
772-774 |
NN |
denotes |
TA |
T12431 |
783-786 |
NN |
denotes |
kit |
T12432 |
775-782 |
NN |
denotes |
cloning |
T12433 |
786-788 |
, |
denotes |
, |
T12434 |
798-799 |
-RRB- |
denotes |
) |
T12435 |
799-801 |
, |
denotes |
, |
T12436 |
801-804 |
CC |
denotes |
and |
T12437 |
805-815 |
JJ |
denotes |
individual |
T12438 |
816-822 |
NNS |
denotes |
clones |
T12439 |
828-837 |
VBN |
denotes |
sequenced |
T12440 |
823-827 |
VBD |
denotes |
were |
T12441 |
837-838 |
. |
denotes |
. |
T12442 |
838-962 |
sentence |
denotes |
Specific PCR primers used to confirm expression of individual olfactory receptor genes are given in Additional data file 1. |
T12443 |
839-847 |
JJ |
denotes |
Specific |
T12444 |
852-859 |
NNS |
denotes |
primers |
T12445 |
848-851 |
NN |
denotes |
PCR |
T12446 |
930-935 |
VBN |
denotes |
given |
T12447 |
860-864 |
VBN |
denotes |
used |
T12448 |
865-867 |
TO |
denotes |
to |
T12449 |
868-875 |
VB |
denotes |
confirm |
T12450 |
876-886 |
NN |
denotes |
expression |
T12451 |
887-889 |
IN |
denotes |
of |
T12452 |
890-900 |
JJ |
denotes |
individual |
T12453 |
920-925 |
NNS |
denotes |
genes |
T12454 |
901-910 |
JJ |
denotes |
olfactory |
T12455 |
911-919 |
NN |
denotes |
receptor |
T12456 |
926-929 |
VBP |
denotes |
are |
T12457 |
936-938 |
IN |
denotes |
in |
T12458 |
939-949 |
JJ |
denotes |
Additional |
T12459 |
955-959 |
NN |
denotes |
file |
T12460 |
950-954 |
NNS |
denotes |
data |
T12461 |
960-961 |
CD |
denotes |
1 |
T12462 |
961-962 |
. |
denotes |
. |
T12463 |
962-1061 |
sentence |
denotes |
Each PCR product was sequenced to confirm that the expected gene and no others had been amplified. |
T12464 |
963-967 |
DT |
denotes |
Each |
T12465 |
972-979 |
NN |
denotes |
product |
T12466 |
968-971 |
NN |
denotes |
PCR |
T12467 |
984-993 |
VBN |
denotes |
sequenced |
T12468 |
980-983 |
VBD |
denotes |
was |
T12469 |
994-996 |
TO |
denotes |
to |
T12470 |
997-1004 |
VB |
denotes |
confirm |
T12471 |
1005-1009 |
IN |
denotes |
that |
T12472 |
1051-1060 |
VBN |
denotes |
amplified |
T12473 |
1010-1013 |
DT |
denotes |
the |
T12474 |
1023-1027 |
NN |
denotes |
gene |
T12475 |
1014-1022 |
VBN |
denotes |
expected |
T12476 |
1028-1031 |
CC |
denotes |
and |
T12477 |
1032-1034 |
DT |
denotes |
no |
T12478 |
1035-1041 |
NNS |
denotes |
others |
T12479 |
1042-1045 |
VBD |
denotes |
had |
T12480 |
1046-1050 |
VBN |
denotes |
been |
T12481 |
1060-1061 |
. |
denotes |
. |
T12482 |
1061-1220 |
sentence |
denotes |
Control reactions on a template made by omitting reverse transcriptase gave no product, indicating that the RNA preparation was uncontaminated by genomic DNA. |
T12483 |
1062-1069 |
NN |
denotes |
Control |
T12484 |
1070-1079 |
NNS |
denotes |
reactions |
T12485 |
1133-1137 |
VBD |
denotes |
gave |
T12486 |
1080-1082 |
IN |
denotes |
on |
T12487 |
1083-1084 |
DT |
denotes |
a |
T12488 |
1085-1093 |
NN |
denotes |
template |
T12489 |
1094-1098 |
VBN |
denotes |
made |
T12490 |
1099-1101 |
IN |
denotes |
by |
T12491 |
1102-1110 |
VBG |
denotes |
omitting |
T12492 |
1111-1118 |
JJ |
denotes |
reverse |
T12493 |
1119-1132 |
NN |
denotes |
transcriptase |
T12494 |
1138-1140 |
DT |
denotes |
no |
T12495 |
1141-1148 |
NN |
denotes |
product |
T12496 |
1148-1150 |
, |
denotes |
, |
T12497 |
1150-1160 |
VBG |
denotes |
indicating |
T12498 |
1161-1165 |
IN |
denotes |
that |
T12499 |
1186-1189 |
VBD |
denotes |
was |
T12500 |
1166-1169 |
DT |
denotes |
the |
T12501 |
1174-1185 |
NN |
denotes |
preparation |
T12502 |
1170-1173 |
NN |
denotes |
RNA |
T12503 |
1190-1204 |
JJ |
denotes |
uncontaminated |
T12504 |
1205-1207 |
IN |
denotes |
by |
T12505 |
1208-1215 |
JJ |
denotes |
genomic |
T12506 |
1216-1219 |
NN |
denotes |
DNA |
T12507 |
1219-1220 |
. |
denotes |
. |
R7752 |
T12273 |
T12274 |
det |
The,epithelia |
R7753 |
T12274 |
T12276 |
nsubjpass |
epithelia,dissected |
R7754 |
T12275 |
T12274 |
amod |
olfactory,epithelia |
R7755 |
T12277 |
T12276 |
auxpass |
were,dissected |
R7756 |
T12278 |
T12276 |
prep |
from,dissected |
R7757 |
T12279 |
T12280 |
nummod |
three,mice |
R7758 |
T12280 |
T12278 |
pobj |
mice,from |
R7759 |
T12281 |
T12280 |
amod |
adult,mice |
R7760 |
T12282 |
T12280 |
amod |
female,mice |
R7761 |
T12283 |
T12280 |
nmod |
C57BL,mice |
R7762 |
T12284 |
T12283 |
punct |
/,C57BL |
R7763 |
T12285 |
T12283 |
nummod |
6,C57BL |
R7764 |
T12286 |
T12276 |
punct |
", ",dissected |
R7765 |
T12287 |
T12276 |
prep |
including,dissected |
R7766 |
T12288 |
T12287 |
pobj |
tissues,including |
R7767 |
T12289 |
T12288 |
acl |
attached,tissues |
R7768 |
T12290 |
T12289 |
prep |
to,attached |
R7769 |
T12291 |
T12292 |
det |
the,skull |
R7770 |
T12292 |
T12290 |
pobj |
skull,to |
R7771 |
T12293 |
T12292 |
cc |
and,skull |
R7772 |
T12294 |
T12292 |
conj |
septum,skull |
R7773 |
T12295 |
T12276 |
punct |
.,dissected |
R7774 |
T12297 |
T12298 |
nsubjpass |
RNA,isolated |
R7775 |
T12299 |
T12298 |
auxpass |
was,isolated |
R7776 |
T12300 |
T12298 |
advcl |
using,isolated |
R7777 |
T12301 |
T12302 |
det |
the,kit |
R7778 |
T12302 |
T12300 |
dobj |
kit,using |
R7779 |
T12303 |
T12302 |
compound |
Qiagen,kit |
R7780 |
T12304 |
T12302 |
compound |
RNeasy,kit |
R7781 |
T12305 |
T12302 |
compound |
midi,kit |
R7782 |
T12306 |
T12307 |
punct |
(,Qiagen |
R7783 |
T12307 |
T12302 |
parataxis |
Qiagen,kit |
R7784 |
T12308 |
T12307 |
punct |
", ",Qiagen |
R7785 |
T12309 |
T12307 |
npadvmod |
Valencia,Qiagen |
R7786 |
T12310 |
T12307 |
punct |
", ",Qiagen |
R7787 |
T12311 |
T12307 |
npadvmod |
CA,Qiagen |
R7788 |
T12312 |
T12307 |
punct |
", ",Qiagen |
R7789 |
T12313 |
T12307 |
npadvmod |
USA,Qiagen |
R7790 |
T12314 |
T12307 |
punct |
),Qiagen |
R7791 |
T12315 |
T12300 |
punct |
", ",using |
R7792 |
T12316 |
T12300 |
prep |
including,using |
R7793 |
T12317 |
T12318 |
det |
a,step |
R7794 |
T12318 |
T12316 |
pobj |
step,including |
R7795 |
T12319 |
T12318 |
compound |
DNase,step |
R7796 |
T12320 |
T12318 |
compound |
treatment,step |
R7797 |
T12321 |
T12298 |
punct |
.,isolated |
R7798 |
T12323 |
T12324 |
amod |
First,strand |
R7799 |
T12324 |
T12326 |
compound |
strand,cDNA |
R7800 |
T12325 |
T12324 |
punct |
-,strand |
R7801 |
T12326 |
T12327 |
nsubjpass |
cDNA,produced |
R7802 |
T12328 |
T12327 |
auxpass |
was,produced |
R7803 |
T12329 |
T12327 |
prep |
from,produced |
R7804 |
T12330 |
T12331 |
nummod |
2.5,μg |
R7805 |
T12331 |
T12329 |
pobj |
μg,from |
R7806 |
T12332 |
T12331 |
prep |
of,μg |
R7807 |
T12333 |
T12332 |
pobj |
RNA,of |
R7808 |
T12334 |
T12331 |
prep |
in,μg |
R7809 |
T12335 |
T12336 |
det |
a,volume |
R7810 |
T12336 |
T12334 |
pobj |
volume,in |
R7811 |
T12337 |
T12336 |
prep |
of,volume |
R7812 |
T12338 |
T12339 |
nummod |
50,μl |
R7813 |
T12339 |
T12337 |
pobj |
μl,of |
R7814 |
T12340 |
T12327 |
advcl |
using,produced |
R7815 |
T12341 |
T12342 |
amod |
random,hexamers |
R7816 |
T12342 |
T12340 |
dobj |
hexamers,using |
R7817 |
T12343 |
T12342 |
cc |
and,hexamers |
R7818 |
T12344 |
T12345 |
poss |
Invitrogen,transcriptase |
R7819 |
T12345 |
T12342 |
conj |
transcriptase,hexamers |
R7820 |
T12346 |
T12344 |
case |
's,Invitrogen |
R7821 |
T12347 |
T12345 |
nmod |
Superscript,transcriptase |
R7822 |
T12348 |
T12347 |
nummod |
II,Superscript |
R7823 |
T12349 |
T12345 |
amod |
reverse,transcriptase |
R7824 |
T12350 |
T12351 |
punct |
(,Invitrogen |
R7825 |
T12351 |
T12345 |
parataxis |
Invitrogen,transcriptase |
R7826 |
T12352 |
T12351 |
punct |
", ",Invitrogen |
R7827 |
T12353 |
T12351 |
npadvmod |
Carlsbad,Invitrogen |
R7828 |
T12354 |
T12351 |
punct |
", ",Invitrogen |
R7829 |
T12355 |
T12351 |
npadvmod |
CA,Invitrogen |
R7830 |
T12356 |
T12351 |
punct |
", ",Invitrogen |
R7831 |
T12357 |
T12351 |
npadvmod |
USA,Invitrogen |
R7832 |
T12358 |
T12351 |
punct |
),Invitrogen |
R7833 |
T12359 |
T12327 |
punct |
", ",produced |
R7834 |
T12360 |
T12327 |
prep |
according,produced |
R7835 |
T12361 |
T12360 |
prep |
to,according |
R7836 |
T12362 |
T12363 |
det |
the,manufacturer |
R7837 |
T12363 |
T12364 |
poss |
manufacturer,recommendations |
R7838 |
T12364 |
T12361 |
pobj |
recommendations,to |
R7839 |
T12365 |
T12363 |
case |
's,manufacturer |
R7840 |
T12366 |
T12327 |
punct |
.,produced |
R7841 |
T12368 |
T12369 |
nummod |
One,fifth |
R7842 |
T12369 |
T12373 |
nsubjpass |
fifth,used |
R7843 |
T12370 |
T12369 |
punct |
-,fifth |
R7844 |
T12371 |
T12369 |
nummod |
twenty,fifth |
R7845 |
T12372 |
T12369 |
punct |
-,fifth |
R7846 |
T12374 |
T12369 |
prep |
of,fifth |
R7847 |
T12375 |
T12376 |
det |
the,cDNA |
R7848 |
T12376 |
T12374 |
pobj |
cDNA,of |
R7849 |
T12377 |
T12376 |
amod |
resulting,cDNA |
R7850 |
T12378 |
T12373 |
auxpass |
was,used |
R7851 |
T12379 |
T12373 |
prep |
as,used |
R7852 |
T12380 |
T12379 |
pobj |
template,as |
R7853 |
T12381 |
T12373 |
prep |
in,used |
R7854 |
T12382 |
T12383 |
amod |
subsequent,reactions |
R7855 |
T12383 |
T12381 |
pobj |
reactions,in |
R7856 |
T12384 |
T12383 |
compound |
PCR,reactions |
R7857 |
T12385 |
T12373 |
punct |
.,used |
R7858 |
T12387 |
T12388 |
compound |
PCR,amplification |
R7859 |
T12388 |
T12389 |
nsubjpass |
amplification,performed |
R7860 |
T12390 |
T12388 |
amod |
biased,amplification |
R7861 |
T12391 |
T12390 |
prep |
towards,biased |
R7862 |
T12392 |
T12393 |
nmod |
class,receptors |
R7863 |
T12393 |
T12391 |
pobj |
receptors,towards |
R7864 |
T12394 |
T12392 |
nummod |
I,class |
R7865 |
T12395 |
T12393 |
amod |
olfactory,receptors |
R7866 |
T12396 |
T12389 |
auxpass |
was,performed |
R7867 |
T12397 |
T12389 |
advcl |
using,performed |
R7868 |
T12398 |
T12399 |
amod |
degenerate,P26 |
R7869 |
T12399 |
T12397 |
dobj |
P26,using |
R7870 |
T12400 |
T12399 |
compound |
primers,P26 |
R7871 |
T12401 |
T12402 |
punct |
[,17 |
R7872 |
T12402 |
T12399 |
parataxis |
17,P26 |
R7873 |
T12403 |
T12402 |
punct |
],17 |
R7874 |
T12404 |
T12399 |
cc |
and,P26 |
R7875 |
T12405 |
T12399 |
conj |
classI_R1,P26 |
R7876 |
T12406 |
T12407 |
punct |
(,GGRTTIADIRYIGGNGG |
R7877 |
T12407 |
T12405 |
parataxis |
GGRTTIADIRYIGGNGG,classI_R1 |
R7878 |
T12408 |
T12407 |
nummod |
5,GGRTTIADIRYIGGNGG |
R7879 |
T12409 |
T12408 |
punct |
',5 |
R7880 |
T12410 |
T12407 |
punct |
-,GGRTTIADIRYIGGNGG |
R7881 |
T12411 |
T12407 |
punct |
-,GGRTTIADIRYIGGNGG |
R7882 |
T12412 |
T12407 |
nummod |
3,GGRTTIADIRYIGGNGG |
R7883 |
T12413 |
T12407 |
punct |
',GGRTTIADIRYIGGNGG |
R7884 |
T12414 |
T12407 |
punct |
),GGRTTIADIRYIGGNGG |
R7885 |
T12415 |
T12399 |
prep |
with,P26 |
R7886 |
T12416 |
T12417 |
det |
an,temperature |
R7887 |
T12417 |
T12415 |
pobj |
temperature,with |
R7888 |
T12418 |
T12417 |
amod |
annealing,temperature |
R7889 |
T12419 |
T12417 |
prep |
of,temperature |
R7890 |
T12420 |
T12421 |
nummod |
44,°C |
R7891 |
T12421 |
T12419 |
pobj |
°C,of |
R7892 |
T12422 |
T12389 |
punct |
.,performed |
R7893 |
T12424 |
T12425 |
det |
The,product |
R7894 |
T12425 |
T12426 |
nsubjpass |
product,cloned |
R7895 |
T12427 |
T12426 |
auxpass |
was,cloned |
R7896 |
T12428 |
T12429 |
punct |
(,Invitrogen |
R7897 |
T12429 |
T12426 |
parataxis |
Invitrogen,cloned |
R7898 |
T12430 |
T12431 |
compound |
TA,kit |
R7899 |
T12431 |
T12429 |
dep |
kit,Invitrogen |
R7900 |
T12432 |
T12431 |
compound |
cloning,kit |
R7901 |
T12433 |
T12429 |
punct |
", ",Invitrogen |
R7902 |
T12434 |
T12429 |
punct |
),Invitrogen |
R7903 |
T12435 |
T12426 |
punct |
", ",cloned |
R7904 |
T12436 |
T12426 |
cc |
and,cloned |
R7905 |
T12437 |
T12438 |
amod |
individual,clones |
R7906 |
T12438 |
T12439 |
nsubjpass |
clones,sequenced |
R7907 |
T12439 |
T12426 |
conj |
sequenced,cloned |
R7908 |
T12440 |
T12439 |
auxpass |
were,sequenced |
R7909 |
T12441 |
T12439 |
punct |
.,sequenced |
R7910 |
T12443 |
T12444 |
amod |
Specific,primers |
R7911 |
T12444 |
T12446 |
nsubjpass |
primers,given |
R7912 |
T12445 |
T12444 |
compound |
PCR,primers |
R7913 |
T12447 |
T12444 |
acl |
used,primers |
R7914 |
T12448 |
T12449 |
aux |
to,confirm |
R7915 |
T12449 |
T12447 |
advcl |
confirm,used |
R7916 |
T12450 |
T12449 |
dobj |
expression,confirm |
R7917 |
T12451 |
T12450 |
prep |
of,expression |
R7918 |
T12452 |
T12453 |
amod |
individual,genes |
R7919 |
T12453 |
T12451 |
pobj |
genes,of |
R7920 |
T12454 |
T12455 |
amod |
olfactory,receptor |
R7921 |
T12455 |
T12453 |
compound |
receptor,genes |
R7922 |
T12456 |
T12446 |
auxpass |
are,given |
R7923 |
T12457 |
T12446 |
prep |
in,given |
R7924 |
T12458 |
T12459 |
amod |
Additional,file |
R7925 |
T12459 |
T12457 |
pobj |
file,in |
R7926 |
T12460 |
T12459 |
compound |
data,file |
R7927 |
T12461 |
T12459 |
nummod |
1,file |
R7928 |
T12462 |
T12446 |
punct |
.,given |
R7929 |
T12464 |
T12465 |
det |
Each,product |
R7930 |
T12465 |
T12467 |
nsubjpass |
product,sequenced |
R7931 |
T12466 |
T12465 |
compound |
PCR,product |
R7932 |
T12468 |
T12467 |
auxpass |
was,sequenced |
R7933 |
T12469 |
T12470 |
aux |
to,confirm |
R7934 |
T12470 |
T12467 |
advcl |
confirm,sequenced |
R7935 |
T12471 |
T12472 |
mark |
that,amplified |
R7936 |
T12472 |
T12470 |
ccomp |
amplified,confirm |
R7937 |
T12473 |
T12474 |
det |
the,gene |
R7938 |
T12474 |
T12472 |
nsubjpass |
gene,amplified |
R7939 |
T12475 |
T12474 |
amod |
expected,gene |
R7940 |
T12476 |
T12474 |
cc |
and,gene |
R7941 |
T12477 |
T12478 |
det |
no,others |
R7942 |
T12478 |
T12474 |
conj |
others,gene |
R7943 |
T12479 |
T12472 |
aux |
had,amplified |
R7944 |
T12480 |
T12472 |
auxpass |
been,amplified |
R7945 |
T12481 |
T12467 |
punct |
.,sequenced |
R7946 |
T12483 |
T12484 |
compound |
Control,reactions |
R7947 |
T12484 |
T12485 |
nsubj |
reactions,gave |
R7948 |
T12486 |
T12484 |
prep |
on,reactions |
R7949 |
T12487 |
T12488 |
det |
a,template |
R7950 |
T12488 |
T12486 |
pobj |
template,on |
R7951 |
T12489 |
T12488 |
acl |
made,template |
R7952 |
T12490 |
T12489 |
prep |
by,made |
R7953 |
T12491 |
T12490 |
pcomp |
omitting,by |
R7954 |
T12492 |
T12493 |
amod |
reverse,transcriptase |
R7955 |
T12493 |
T12491 |
dobj |
transcriptase,omitting |
R7956 |
T12494 |
T12495 |
det |
no,product |
R7957 |
T12495 |
T12485 |
dobj |
product,gave |
R7958 |
T12496 |
T12485 |
punct |
", ",gave |
R7959 |
T12497 |
T12485 |
advcl |
indicating,gave |
R7960 |
T12498 |
T12499 |
mark |
that,was |
R7961 |
T12499 |
T12497 |
ccomp |
was,indicating |
R7962 |
T12500 |
T12501 |
det |
the,preparation |
R7963 |
T12501 |
T12499 |
nsubj |
preparation,was |
R7964 |
T12502 |
T12501 |
compound |
RNA,preparation |
R7965 |
T12503 |
T12499 |
acomp |
uncontaminated,was |
R7966 |
T12504 |
T12503 |
prep |
by,uncontaminated |
R7967 |
T12505 |
T12506 |
amod |
genomic,DNA |
R7968 |
T12506 |
T12504 |
pobj |
DNA,by |
R7969 |
T12507 |
T12485 |
punct |
.,gave |