Id |
Subject |
Object |
Predicate |
Lexical cue |
T10707 |
242-249 |
NNS |
denotes |
embryos |
T10708 |
230-235 |
NN |
denotes |
E16.5 |
T10663 |
0-2 |
DT |
denotes |
An |
T10664 |
20-27 |
NN |
denotes |
library |
T10665 |
3-8 |
JJ |
denotes |
adult |
T10666 |
9-14 |
NN |
denotes |
mouse |
T10667 |
15-19 |
NN |
denotes |
cDNA |
T10668 |
86-94 |
VBN |
denotes |
provided |
T10669 |
28-32 |
VBN |
denotes |
made |
T10670 |
33-37 |
IN |
denotes |
from |
T10671 |
38-41 |
DT |
denotes |
the |
T10672 |
52-62 |
NN |
denotes |
epithelium |
T10673 |
42-51 |
JJ |
denotes |
olfactory |
T10674 |
63-65 |
IN |
denotes |
of |
T10675 |
66-67 |
DT |
denotes |
a |
T10676 |
75-81 |
NN |
denotes |
animal |
T10677 |
68-74 |
JJ |
denotes |
single |
T10678 |
82-85 |
VBD |
denotes |
was |
T10679 |
95-97 |
IN |
denotes |
by |
T10680 |
98-104 |
NNP |
denotes |
Leslie |
T10681 |
105-113 |
NNP |
denotes |
Vosshall |
T10682 |
114-115 |
-LRB- |
denotes |
( |
T10683 |
127-137 |
NNP |
denotes |
University |
T10684 |
115-126 |
NNP |
denotes |
Rockefeller |
T10685 |
137-139 |
, |
denotes |
, |
T10686 |
139-142 |
NNP |
denotes |
New |
T10687 |
143-147 |
NNP |
denotes |
York |
T10688 |
147-149 |
, |
denotes |
, |
T10689 |
149-151 |
NNP |
denotes |
NY |
T10690 |
151-153 |
, |
denotes |
, |
T10691 |
153-156 |
NNP |
denotes |
USA |
T10692 |
156-157 |
-RRB- |
denotes |
) |
T10693 |
157-159 |
, |
denotes |
, |
T10694 |
159-162 |
CC |
denotes |
and |
T10695 |
163-165 |
DT |
denotes |
an |
T10696 |
176-183 |
NN |
denotes |
library |
T10697 |
166-175 |
JJ |
denotes |
embryonic |
T10698 |
255-263 |
VBN |
denotes |
provided |
T10699 |
184-185 |
-LRB- |
denotes |
( |
T10700 |
185-189 |
VBN |
denotes |
made |
T10701 |
190-194 |
IN |
denotes |
from |
T10702 |
195-198 |
DT |
denotes |
the |
T10703 |
209-218 |
NNS |
denotes |
epithelia |
T10704 |
199-208 |
JJ |
denotes |
olfactory |
T10705 |
219-221 |
IN |
denotes |
of |
T10706 |
222-229 |
JJ |
denotes |
several |
T10709 |
236-241 |
NN |
denotes |
E18.5 |
T10710 |
235-236 |
HYPH |
denotes |
- |
T10711 |
249-250 |
-RRB- |
denotes |
) |
T10712 |
251-254 |
VBD |
denotes |
was |
T10713 |
264-266 |
IN |
denotes |
by |
T10714 |
267-272 |
NNP |
denotes |
Tyler |
T10715 |
273-281 |
NNP |
denotes |
Cutforth |
T10716 |
282-283 |
-LRB- |
denotes |
( |
T10717 |
292-302 |
NNP |
denotes |
University |
T10718 |
283-291 |
NNP |
denotes |
Columbia |
T10719 |
302-304 |
, |
denotes |
, |
T10720 |
304-307 |
NNP |
denotes |
New |
T10721 |
308-312 |
NNP |
denotes |
York |
T10722 |
312-314 |
, |
denotes |
, |
T10723 |
314-316 |
NNP |
denotes |
NY |
T10724 |
316-318 |
, |
denotes |
, |
T10725 |
318-321 |
NNP |
denotes |
USA |
T10726 |
321-322 |
-RRB- |
denotes |
) |
T10727 |
322-323 |
. |
denotes |
. |
T10728 |
323-446 |
sentence |
denotes |
Both libraries were oligo-dT primed and directionally cloned into the lambdaZAP-XR vector (Stratagene, La Jolla, CA, USA). |
T10729 |
324-328 |
DT |
denotes |
Both |
T10730 |
329-338 |
NNS |
denotes |
libraries |
T10731 |
353-359 |
VBN |
denotes |
primed |
T10732 |
339-343 |
VBD |
denotes |
were |
T10733 |
344-349 |
NN |
denotes |
oligo |
T10734 |
349-350 |
HYPH |
denotes |
- |
T10735 |
350-352 |
NN |
denotes |
dT |
T10736 |
360-363 |
CC |
denotes |
and |
T10737 |
364-377 |
RB |
denotes |
directionally |
T10738 |
378-384 |
VBN |
denotes |
cloned |
T10739 |
385-389 |
IN |
denotes |
into |
T10740 |
390-393 |
DT |
denotes |
the |
T10741 |
407-413 |
NN |
denotes |
vector |
T10742 |
394-403 |
NN |
denotes |
lambdaZAP |
T10743 |
404-406 |
NN |
denotes |
XR |
T10744 |
403-404 |
HYPH |
denotes |
- |
T10745 |
414-415 |
-LRB- |
denotes |
( |
T10746 |
415-425 |
NNP |
denotes |
Stratagene |
T10747 |
425-427 |
, |
denotes |
, |
T10748 |
427-429 |
NNP |
denotes |
La |
T10749 |
430-435 |
NNP |
denotes |
Jolla |
T10750 |
435-437 |
, |
denotes |
, |
T10751 |
437-439 |
NNP |
denotes |
CA |
T10752 |
439-441 |
, |
denotes |
, |
T10753 |
441-444 |
NNP |
denotes |
USA |
T10754 |
444-445 |
-RRB- |
denotes |
) |
T10755 |
445-446 |
. |
denotes |
. |
T10756 |
446-568 |
sentence |
denotes |
The adult library has a complexity of 6.5 × 106 primary clones, and the embryonic library has a complexity of 1.65 × 106. |
T10757 |
447-450 |
DT |
denotes |
The |
T10758 |
457-464 |
NN |
denotes |
library |
T10759 |
451-456 |
JJ |
denotes |
adult |
T10760 |
465-468 |
VBZ |
denotes |
has |
T10761 |
469-470 |
DT |
denotes |
a |
T10762 |
471-481 |
NN |
denotes |
complexity |
T10763 |
482-484 |
IN |
denotes |
of |
T10764 |
485-488 |
CD |
denotes |
6.5 |
T10765 |
491-494 |
CD |
denotes |
106 |
T10766 |
489-490 |
SYM |
denotes |
× |
T10767 |
503-509 |
NNS |
denotes |
clones |
T10768 |
495-502 |
JJ |
denotes |
primary |
T10769 |
509-511 |
, |
denotes |
, |
T10770 |
511-514 |
CC |
denotes |
and |
T10771 |
515-518 |
DT |
denotes |
the |
T10772 |
529-536 |
NN |
denotes |
library |
T10773 |
519-528 |
JJ |
denotes |
embryonic |
T10774 |
537-540 |
VBZ |
denotes |
has |
T10775 |
541-542 |
DT |
denotes |
a |
T10776 |
543-553 |
NN |
denotes |
complexity |
T10777 |
554-556 |
IN |
denotes |
of |
T10778 |
557-561 |
CD |
denotes |
1.65 |
T10779 |
564-567 |
CD |
denotes |
106 |
T10780 |
562-563 |
SYM |
denotes |
× |
T10781 |
567-568 |
. |
denotes |
. |
T10782 |
568-666 |
sentence |
denotes |
Libraries were amplified to give titers of 5 × 109 pfu/ml (adult) or 2 × 1010 pfu/ml (embryonic). |
T10783 |
569-578 |
NNS |
denotes |
Libraries |
T10784 |
584-593 |
VBN |
denotes |
amplified |
T10785 |
579-583 |
VBD |
denotes |
were |
T10786 |
594-596 |
TO |
denotes |
to |
T10787 |
597-601 |
VB |
denotes |
give |
T10788 |
602-608 |
NNS |
denotes |
titers |
T10789 |
609-611 |
IN |
denotes |
of |
T10790 |
612-613 |
CD |
denotes |
5 |
T10791 |
616-619 |
CD |
denotes |
109 |
T10792 |
614-615 |
SYM |
denotes |
× |
T10793 |
620-623 |
NNS |
denotes |
pfu |
T10794 |
623-624 |
SYM |
denotes |
/ |
T10795 |
624-626 |
NN |
denotes |
ml |
T10796 |
627-628 |
-LRB- |
denotes |
( |
T10797 |
628-633 |
JJ |
denotes |
adult |
T10798 |
633-634 |
-RRB- |
denotes |
) |
T10799 |
635-637 |
CC |
denotes |
or |
T10800 |
638-639 |
CD |
denotes |
2 |
T10801 |
642-646 |
CD |
denotes |
1010 |
T10802 |
640-641 |
SYM |
denotes |
× |
T10803 |
647-650 |
NNS |
denotes |
pfu |
T10804 |
650-651 |
SYM |
denotes |
/ |
T10805 |
651-653 |
NN |
denotes |
ml |
T10806 |
654-655 |
-LRB- |
denotes |
( |
T10807 |
655-664 |
JJ |
denotes |
embryonic |
T10808 |
664-665 |
-RRB- |
denotes |
) |
T10809 |
665-666 |
. |
denotes |
. |
T10810 |
666-856 |
sentence |
denotes |
Hybridization probes were made by degenerate PCR of mouse genomic DNA, in a fashion similar to those described previously [1], with primer pairs and annealing temperatures given in Table 2. |
T10811 |
667-680 |
NN |
denotes |
Hybridization |
T10812 |
681-687 |
NNS |
denotes |
probes |
T10813 |
693-697 |
VBN |
denotes |
made |
T10814 |
688-692 |
VBD |
denotes |
were |
T10815 |
698-700 |
IN |
denotes |
by |
T10816 |
701-711 |
JJ |
denotes |
degenerate |
T10817 |
712-715 |
NN |
denotes |
PCR |
T10818 |
716-718 |
IN |
denotes |
of |
T10819 |
719-724 |
NN |
denotes |
mouse |
T10820 |
733-736 |
NN |
denotes |
DNA |
T10821 |
725-732 |
JJ |
denotes |
genomic |
T10822 |
736-738 |
, |
denotes |
, |
T10823 |
738-740 |
IN |
denotes |
in |
T10824 |
741-742 |
DT |
denotes |
a |
T10825 |
743-750 |
NN |
denotes |
fashion |
T10826 |
751-758 |
JJ |
denotes |
similar |
T10827 |
759-761 |
IN |
denotes |
to |
T10828 |
762-767 |
DT |
denotes |
those |
T10829 |
768-777 |
VBN |
denotes |
described |
T10830 |
778-788 |
RB |
denotes |
previously |
T10831 |
789-790 |
-LRB- |
denotes |
[ |
T10832 |
790-791 |
CD |
denotes |
1 |
T10833 |
791-792 |
-RRB- |
denotes |
] |
T10834 |
792-794 |
, |
denotes |
, |
T10835 |
794-798 |
IN |
denotes |
with |
T10836 |
839-844 |
VBN |
denotes |
given |
T10837 |
799-805 |
NN |
denotes |
primer |
T10838 |
806-811 |
NNS |
denotes |
pairs |
T10839 |
812-815 |
CC |
denotes |
and |
T10840 |
816-825 |
VBG |
denotes |
annealing |
T10841 |
826-838 |
NNS |
denotes |
temperatures |
T10842 |
845-847 |
IN |
denotes |
in |
T10843 |
848-853 |
NN |
denotes |
Table |
T10844 |
854-855 |
CD |
denotes |
2 |
T10845 |
855-856 |
. |
denotes |
. |
T10846 |
856-919 |
sentence |
denotes |
Low-stringency hybridization conditions were as described [1]. |
T10847 |
857-860 |
JJ |
denotes |
Low |
T10848 |
861-871 |
NN |
denotes |
stringency |
T10849 |
860-861 |
HYPH |
denotes |
- |
T10850 |
886-896 |
NNS |
denotes |
conditions |
T10851 |
872-885 |
NN |
denotes |
hybridization |
T10852 |
897-901 |
VBD |
denotes |
were |
T10853 |
902-904 |
IN |
denotes |
as |
T10854 |
905-914 |
VBN |
denotes |
described |
T10855 |
915-916 |
-LRB- |
denotes |
[ |
T10856 |
916-917 |
CD |
denotes |
1 |
T10857 |
917-918 |
-RRB- |
denotes |
] |
T10858 |
918-919 |
. |
denotes |
. |
T10859 |
919-1039 |
sentence |
denotes |
Clonally-pure plaques were obtained through secondary screens using the same probe as the corresponding primary screen. |
T10860 |
920-928 |
RB |
denotes |
Clonally |
T10861 |
929-933 |
JJ |
denotes |
pure |
T10862 |
928-929 |
HYPH |
denotes |
- |
T10863 |
934-941 |
NNS |
denotes |
plaques |
T10864 |
947-955 |
VBN |
denotes |
obtained |
T10865 |
942-946 |
VBD |
denotes |
were |
T10866 |
956-963 |
IN |
denotes |
through |
T10867 |
964-973 |
JJ |
denotes |
secondary |
T10868 |
974-981 |
NNS |
denotes |
screens |
T10869 |
982-987 |
VBG |
denotes |
using |
T10870 |
988-991 |
DT |
denotes |
the |
T10871 |
997-1002 |
NN |
denotes |
probe |
T10872 |
992-996 |
JJ |
denotes |
same |
T10873 |
1003-1005 |
IN |
denotes |
as |
T10874 |
1006-1009 |
DT |
denotes |
the |
T10875 |
1032-1038 |
NN |
denotes |
screen |
T10876 |
1010-1023 |
VBG |
denotes |
corresponding |
T10877 |
1024-1031 |
JJ |
denotes |
primary |
T10878 |
1038-1039 |
. |
denotes |
. |
T10879 |
1039-1119 |
sentence |
denotes |
PCR with vector primers (M13F/R) was performed to prepare sequencing templates. |
T10880 |
1040-1043 |
NN |
denotes |
PCR |
T10881 |
1077-1086 |
VBN |
denotes |
performed |
T10882 |
1044-1048 |
IN |
denotes |
with |
T10883 |
1049-1055 |
NN |
denotes |
vector |
T10884 |
1056-1063 |
NNS |
denotes |
primers |
T10885 |
1064-1065 |
-LRB- |
denotes |
( |
T10886 |
1070-1071 |
NN |
denotes |
R |
T10887 |
1065-1069 |
NN |
denotes |
M13F |
T10888 |
1069-1070 |
HYPH |
denotes |
/ |
T10889 |
1071-1072 |
-RRB- |
denotes |
) |
T10890 |
1073-1076 |
VBD |
denotes |
was |
T10891 |
1087-1089 |
TO |
denotes |
to |
T10892 |
1090-1097 |
VB |
denotes |
prepare |
T10893 |
1098-1108 |
NN |
denotes |
sequencing |
T10894 |
1109-1118 |
NNS |
denotes |
templates |
T10895 |
1118-1119 |
. |
denotes |
. |
T10896 |
1119-1356 |
sentence |
denotes |
cDNA size estimates were obtained by agarose gel electrophoresis, and inserts were sequenced from the 5' end using the M13R primer and big-dye terminator chemistry according to ABI's protocols (Applied Biosystems, Foster City, CA, USA). |
T10897 |
1120-1124 |
NN |
denotes |
cDNA |
T10898 |
1125-1129 |
NN |
denotes |
size |
T10899 |
1130-1139 |
NNS |
denotes |
estimates |
T10900 |
1145-1153 |
VBN |
denotes |
obtained |
T10901 |
1140-1144 |
VBD |
denotes |
were |
T10902 |
1154-1156 |
IN |
denotes |
by |
T10903 |
1157-1164 |
NN |
denotes |
agarose |
T10904 |
1165-1168 |
NN |
denotes |
gel |
T10905 |
1169-1184 |
NN |
denotes |
electrophoresis |
T10906 |
1184-1186 |
, |
denotes |
, |
T10907 |
1186-1189 |
CC |
denotes |
and |
T10908 |
1190-1197 |
NNS |
denotes |
inserts |
T10909 |
1203-1212 |
VBN |
denotes |
sequenced |
T10910 |
1198-1202 |
VBD |
denotes |
were |
T10911 |
1213-1217 |
IN |
denotes |
from |
T10912 |
1218-1221 |
DT |
denotes |
the |
T10913 |
1225-1228 |
NN |
denotes |
end |
T10914 |
1222-1223 |
CD |
denotes |
5 |
T10915 |
1223-1224 |
SYM |
denotes |
' |
T10916 |
1229-1234 |
VBG |
denotes |
using |
T10917 |
1235-1238 |
DT |
denotes |
the |
T10918 |
1244-1250 |
NN |
denotes |
primer |
T10919 |
1239-1243 |
NN |
denotes |
M13R |
T10920 |
1251-1254 |
CC |
denotes |
and |
T10921 |
1255-1258 |
JJ |
denotes |
big |
T10922 |
1259-1262 |
NN |
denotes |
dye |
T10923 |
1258-1259 |
HYPH |
denotes |
- |
T10924 |
1274-1283 |
NN |
denotes |
chemistry |
T10925 |
1263-1273 |
NN |
denotes |
terminator |
T10926 |
1284-1293 |
VBG |
denotes |
according |
T10927 |
1294-1296 |
IN |
denotes |
to |
T10928 |
1297-1300 |
NNP |
denotes |
ABI |
T10929 |
1303-1312 |
NNS |
denotes |
protocols |
T10930 |
1300-1302 |
POS |
denotes |
's |
T10931 |
1313-1314 |
-LRB- |
denotes |
( |
T10932 |
1322-1332 |
NNP |
denotes |
Biosystems |
T10933 |
1314-1321 |
NNP |
denotes |
Applied |
T10934 |
1332-1334 |
, |
denotes |
, |
T10935 |
1334-1340 |
NNP |
denotes |
Foster |
T10936 |
1341-1345 |
NNP |
denotes |
City |
T10937 |
1345-1347 |
, |
denotes |
, |
T10938 |
1347-1349 |
NNP |
denotes |
CA |
T10939 |
1349-1351 |
, |
denotes |
, |
T10940 |
1351-1354 |
NNP |
denotes |
USA |
T10941 |
1354-1355 |
-RRB- |
denotes |
) |
T10942 |
1355-1356 |
. |
denotes |
. |
T10943 |
1356-1518 |
sentence |
denotes |
In order to obtain 3' sequence, selected phage clones were converted to plasmid stocks following a scaled-down version of Stratagene's in vivo excision protocol. |
T10944 |
1357-1359 |
IN |
denotes |
In |
T10945 |
1416-1425 |
VBN |
denotes |
converted |
T10946 |
1360-1365 |
NN |
denotes |
order |
T10947 |
1366-1368 |
TO |
denotes |
to |
T10948 |
1369-1375 |
VB |
denotes |
obtain |
T10949 |
1376-1377 |
CD |
denotes |
3 |
T10950 |
1379-1387 |
NN |
denotes |
sequence |
T10951 |
1377-1378 |
SYM |
denotes |
' |
T10952 |
1387-1389 |
, |
denotes |
, |
T10953 |
1389-1397 |
VBN |
denotes |
selected |
T10954 |
1404-1410 |
NNS |
denotes |
clones |
T10955 |
1398-1403 |
NN |
denotes |
phage |
T10956 |
1411-1415 |
VBD |
denotes |
were |
T10957 |
1426-1428 |
IN |
denotes |
to |
T10958 |
1429-1436 |
NN |
denotes |
plasmid |
T10959 |
1437-1443 |
NNS |
denotes |
stocks |
T10960 |
1444-1453 |
VBG |
denotes |
following |
T10961 |
1454-1455 |
DT |
denotes |
a |
T10962 |
1468-1475 |
NN |
denotes |
version |
T10963 |
1456-1462 |
VBN |
denotes |
scaled |
T10964 |
1462-1463 |
HYPH |
denotes |
- |
T10965 |
1463-1467 |
RP |
denotes |
down |
T10966 |
1476-1478 |
IN |
denotes |
of |
T10967 |
1479-1489 |
NNP |
denotes |
Stratagene |
T10968 |
1509-1517 |
NN |
denotes |
protocol |
T10969 |
1489-1491 |
POS |
denotes |
's |
T10970 |
1492-1494 |
FW |
denotes |
in |
T10971 |
1495-1499 |
FW |
denotes |
vivo |
T10972 |
1500-1508 |
NN |
denotes |
excision |
T10973 |
1517-1518 |
. |
denotes |
. |
T10974 |
1518-1652 |
sentence |
denotes |
Plasmid DNA gave better 3'-end sequence than PCR products, which often suffered from polymerase stuttering through the poly(A) tract. |
T10975 |
1519-1526 |
NN |
denotes |
Plasmid |
T10976 |
1527-1530 |
NN |
denotes |
DNA |
T10977 |
1531-1535 |
VBD |
denotes |
gave |
T10978 |
1536-1542 |
JJR |
denotes |
better |
T10979 |
1550-1558 |
NN |
denotes |
sequence |
T10980 |
1543-1544 |
CD |
denotes |
3 |
T10981 |
1546-1549 |
NN |
denotes |
end |
T10982 |
1544-1545 |
SYM |
denotes |
' |
T10983 |
1545-1546 |
HYPH |
denotes |
- |
T10984 |
1559-1563 |
IN |
denotes |
than |
T10985 |
1564-1567 |
NN |
denotes |
PCR |
T10986 |
1568-1576 |
NNS |
denotes |
products |
T10987 |
1576-1578 |
, |
denotes |
, |
T10988 |
1578-1583 |
WDT |
denotes |
which |
T10989 |
1590-1598 |
VBD |
denotes |
suffered |
T10990 |
1584-1589 |
RB |
denotes |
often |
T10991 |
1599-1603 |
IN |
denotes |
from |
T10992 |
1604-1614 |
NN |
denotes |
polymerase |
T10993 |
1615-1625 |
VBG |
denotes |
stuttering |
T10994 |
1626-1633 |
IN |
denotes |
through |
T10995 |
1634-1637 |
DT |
denotes |
the |
T10996 |
1646-1651 |
NN |
denotes |
tract |
T10997 |
1638-1642 |
NN |
denotes |
poly |
T10998 |
1642-1643 |
-LRB- |
denotes |
( |
T10999 |
1643-1644 |
NN |
denotes |
A |
T11000 |
1644-1645 |
-RRB- |
denotes |
) |
T11001 |
1651-1652 |
. |
denotes |
. |
R6566 |
T10663 |
T10664 |
det |
An,library |
R6567 |
T10664 |
T10668 |
nsubjpass |
library,provided |
R6568 |
T10665 |
T10666 |
amod |
adult,mouse |
R6569 |
T10666 |
T10667 |
compound |
mouse,cDNA |
R6570 |
T10667 |
T10664 |
compound |
cDNA,library |
R6571 |
T10669 |
T10664 |
acl |
made,library |
R6572 |
T10670 |
T10669 |
prep |
from,made |
R6573 |
T10671 |
T10672 |
det |
the,epithelium |
R6574 |
T10672 |
T10670 |
pobj |
epithelium,from |
R6575 |
T10673 |
T10672 |
amod |
olfactory,epithelium |
R6576 |
T10674 |
T10672 |
prep |
of,epithelium |
R6577 |
T10675 |
T10676 |
det |
a,animal |
R6578 |
T10676 |
T10674 |
pobj |
animal,of |
R6579 |
T10677 |
T10676 |
amod |
single,animal |
R6580 |
T10678 |
T10668 |
auxpass |
was,provided |
R6581 |
T10679 |
T10668 |
agent |
by,provided |
R6582 |
T10680 |
T10681 |
compound |
Leslie,Vosshall |
R6583 |
T10681 |
T10679 |
pobj |
Vosshall,by |
R6584 |
T10682 |
T10683 |
punct |
(,University |
R6585 |
T10683 |
T10681 |
parataxis |
University,Vosshall |
R6586 |
T10684 |
T10683 |
compound |
Rockefeller,University |
R6587 |
T10685 |
T10683 |
punct |
", ",University |
R6588 |
T10686 |
T10687 |
compound |
New,York |
R6589 |
T10687 |
T10683 |
npadvmod |
York,University |
R6590 |
T10688 |
T10683 |
punct |
", ",University |
R6591 |
T10689 |
T10683 |
npadvmod |
NY,University |
R6592 |
T10690 |
T10683 |
punct |
", ",University |
R6593 |
T10691 |
T10683 |
npadvmod |
USA,University |
R6594 |
T10692 |
T10683 |
punct |
),University |
R6595 |
T10693 |
T10668 |
punct |
", ",provided |
R6596 |
T10694 |
T10668 |
cc |
and,provided |
R6597 |
T10695 |
T10696 |
det |
an,library |
R6598 |
T10696 |
T10698 |
nsubjpass |
library,provided |
R6599 |
T10697 |
T10696 |
amod |
embryonic,library |
R6600 |
T10698 |
T10668 |
conj |
provided,provided |
R6601 |
T10699 |
T10696 |
punct |
(,library |
R6602 |
T10700 |
T10696 |
acl |
made,library |
R6603 |
T10701 |
T10700 |
prep |
from,made |
R6604 |
T10702 |
T10703 |
det |
the,epithelia |
R6605 |
T10703 |
T10701 |
pobj |
epithelia,from |
R6606 |
T10704 |
T10703 |
amod |
olfactory,epithelia |
R6607 |
T10705 |
T10703 |
prep |
of,epithelia |
R6608 |
T10706 |
T10707 |
amod |
several,embryos |
R6609 |
T10707 |
T10705 |
pobj |
embryos,of |
R6610 |
T10708 |
T10709 |
compound |
E16.5,E18.5 |
R6611 |
T10709 |
T10707 |
compound |
E18.5,embryos |
R6612 |
T10710 |
T10709 |
punct |
-,E18.5 |
R6613 |
T10711 |
T10698 |
punct |
),provided |
R6614 |
T10712 |
T10698 |
auxpass |
was,provided |
R6615 |
T10713 |
T10698 |
agent |
by,provided |
R6616 |
T10714 |
T10715 |
compound |
Tyler,Cutforth |
R6617 |
T10715 |
T10713 |
pobj |
Cutforth,by |
R6618 |
T10716 |
T10717 |
punct |
(,University |
R6619 |
T10717 |
T10715 |
parataxis |
University,Cutforth |
R6620 |
T10718 |
T10717 |
compound |
Columbia,University |
R6621 |
T10719 |
T10717 |
punct |
", ",University |
R6622 |
T10720 |
T10721 |
compound |
New,York |
R6623 |
T10721 |
T10717 |
npadvmod |
York,University |
R6624 |
T10722 |
T10717 |
punct |
", ",University |
R6625 |
T10723 |
T10717 |
npadvmod |
NY,University |
R6626 |
T10724 |
T10717 |
punct |
", ",University |
R6627 |
T10725 |
T10717 |
npadvmod |
USA,University |
R6628 |
T10726 |
T10717 |
punct |
),University |
R6629 |
T10727 |
T10698 |
punct |
.,provided |
R6630 |
T10729 |
T10730 |
det |
Both,libraries |
R6631 |
T10730 |
T10731 |
nsubjpass |
libraries,primed |
R6632 |
T10732 |
T10731 |
auxpass |
were,primed |
R6633 |
T10733 |
T10731 |
dep |
oligo,primed |
R6634 |
T10734 |
T10731 |
punct |
-,primed |
R6635 |
T10735 |
T10731 |
dep |
dT,primed |
R6636 |
T10736 |
T10731 |
cc |
and,primed |
R6637 |
T10737 |
T10738 |
advmod |
directionally,cloned |
R6638 |
T10738 |
T10731 |
conj |
cloned,primed |
R6639 |
T10739 |
T10738 |
prep |
into,cloned |
R6640 |
T10740 |
T10741 |
det |
the,vector |
R6641 |
T10741 |
T10739 |
pobj |
vector,into |
R6642 |
T10742 |
T10743 |
compound |
lambdaZAP,XR |
R6643 |
T10743 |
T10741 |
compound |
XR,vector |
R6644 |
T10744 |
T10743 |
punct |
-,XR |
R6645 |
T10745 |
T10746 |
punct |
(,Stratagene |
R6646 |
T10746 |
T10741 |
parataxis |
Stratagene,vector |
R6647 |
T10747 |
T10746 |
punct |
", ",Stratagene |
R6648 |
T10748 |
T10749 |
compound |
La,Jolla |
R6649 |
T10749 |
T10746 |
npadvmod |
Jolla,Stratagene |
R6650 |
T10750 |
T10746 |
punct |
", ",Stratagene |
R6651 |
T10751 |
T10746 |
npadvmod |
CA,Stratagene |
R6652 |
T10752 |
T10746 |
punct |
", ",Stratagene |
R6653 |
T10753 |
T10746 |
npadvmod |
USA,Stratagene |
R6654 |
T10754 |
T10746 |
punct |
),Stratagene |
R6655 |
T10755 |
T10731 |
punct |
.,primed |
R6656 |
T10757 |
T10758 |
det |
The,library |
R6657 |
T10758 |
T10760 |
nsubj |
library,has |
R6658 |
T10759 |
T10758 |
amod |
adult,library |
R6659 |
T10761 |
T10762 |
det |
a,complexity |
R6660 |
T10762 |
T10760 |
dobj |
complexity,has |
R6661 |
T10763 |
T10762 |
prep |
of,complexity |
R6662 |
T10764 |
T10765 |
quantmod |
6.5,106 |
R6663 |
T10765 |
T10767 |
nummod |
106,clones |
R6664 |
T10766 |
T10765 |
punct |
×,106 |
R6665 |
T10767 |
T10763 |
pobj |
clones,of |
R6666 |
T10768 |
T10767 |
amod |
primary,clones |
R6667 |
T10769 |
T10760 |
punct |
", ",has |
R6668 |
T10770 |
T10760 |
cc |
and,has |
R6669 |
T10771 |
T10772 |
det |
the,library |
R6670 |
T10772 |
T10774 |
nsubj |
library,has |
R6671 |
T10773 |
T10772 |
amod |
embryonic,library |
R6672 |
T10774 |
T10760 |
conj |
has,has |
R6673 |
T10775 |
T10776 |
det |
a,complexity |
R6674 |
T10776 |
T10774 |
dobj |
complexity,has |
R6675 |
T10777 |
T10776 |
prep |
of,complexity |
R6676 |
T10778 |
T10779 |
quantmod |
1.65,106 |
R6677 |
T10779 |
T10777 |
pobj |
106,of |
R6678 |
T10780 |
T10779 |
punct |
×,106 |
R6679 |
T10781 |
T10774 |
punct |
.,has |
R6680 |
T10783 |
T10784 |
nsubjpass |
Libraries,amplified |
R6681 |
T10785 |
T10784 |
auxpass |
were,amplified |
R6682 |
T10786 |
T10787 |
aux |
to,give |
R6683 |
T10787 |
T10784 |
advcl |
give,amplified |
R6684 |
T10788 |
T10787 |
dobj |
titers,give |
R6685 |
T10789 |
T10788 |
prep |
of,titers |
R6686 |
T10790 |
T10791 |
quantmod |
5,109 |
R6687 |
T10791 |
T10793 |
nummod |
109,pfu |
R6688 |
T10792 |
T10791 |
punct |
×,109 |
R6689 |
T10793 |
T10789 |
pobj |
pfu,of |
R6690 |
T10794 |
T10795 |
punct |
/,ml |
R6691 |
T10795 |
T10793 |
prep |
ml,pfu |
R6692 |
T10796 |
T10797 |
punct |
(,adult |
R6693 |
T10797 |
T10793 |
parataxis |
adult,pfu |
R6694 |
T10798 |
T10797 |
punct |
),adult |
R6695 |
T10799 |
T10793 |
cc |
or,pfu |
R6696 |
T10800 |
T10801 |
quantmod |
2,1010 |
R6697 |
T10801 |
T10803 |
nummod |
1010,pfu |
R6698 |
T10802 |
T10801 |
punct |
×,1010 |
R6699 |
T10803 |
T10793 |
conj |
pfu,pfu |
R6700 |
T10804 |
T10805 |
punct |
/,ml |
R6701 |
T10805 |
T10803 |
prep |
ml,pfu |
R6702 |
T10806 |
T10807 |
punct |
(,embryonic |
R6703 |
T10807 |
T10803 |
parataxis |
embryonic,pfu |
R6704 |
T10808 |
T10807 |
punct |
),embryonic |
R6705 |
T10809 |
T10784 |
punct |
.,amplified |
R6706 |
T10811 |
T10812 |
compound |
Hybridization,probes |
R6707 |
T10812 |
T10813 |
nsubjpass |
probes,made |
R6708 |
T10814 |
T10813 |
auxpass |
were,made |
R6709 |
T10815 |
T10813 |
prep |
by,made |
R6710 |
T10816 |
T10817 |
amod |
degenerate,PCR |
R6711 |
T10817 |
T10815 |
pobj |
PCR,by |
R6712 |
T10818 |
T10817 |
prep |
of,PCR |
R6713 |
T10819 |
T10820 |
nmod |
mouse,DNA |
R6714 |
T10820 |
T10818 |
pobj |
DNA,of |
R6715 |
T10821 |
T10820 |
amod |
genomic,DNA |
R6716 |
T10822 |
T10813 |
punct |
", ",made |
R6717 |
T10823 |
T10813 |
prep |
in,made |
R6718 |
T10824 |
T10825 |
det |
a,fashion |
R6719 |
T10825 |
T10823 |
pobj |
fashion,in |
R6720 |
T10826 |
T10825 |
amod |
similar,fashion |
R6721 |
T10827 |
T10825 |
prep |
to,fashion |
R6722 |
T10828 |
T10827 |
pobj |
those,to |
R6723 |
T10829 |
T10828 |
acl |
described,those |
R6724 |
T10830 |
T10829 |
advmod |
previously,described |
R6725 |
T10831 |
T10832 |
punct |
[,1 |
R6726 |
T10832 |
T10829 |
parataxis |
1,described |
R6727 |
T10833 |
T10832 |
punct |
],1 |
R6728 |
T10834 |
T10813 |
punct |
", ",made |
R6729 |
T10835 |
T10836 |
mark |
with,given |
R6730 |
T10836 |
T10813 |
advcl |
given,made |
R6731 |
T10837 |
T10838 |
compound |
primer,pairs |
R6732 |
T10838 |
T10836 |
nsubj |
pairs,given |
R6733 |
T10839 |
T10838 |
cc |
and,pairs |
R6734 |
T10840 |
T10841 |
amod |
annealing,temperatures |
R6735 |
T10841 |
T10838 |
conj |
temperatures,pairs |
R6736 |
T10842 |
T10836 |
prep |
in,given |
R6737 |
T10843 |
T10842 |
pobj |
Table,in |
R6738 |
T10844 |
T10843 |
nummod |
2,Table |
R6739 |
T10845 |
T10813 |
punct |
.,made |
R6740 |
T10847 |
T10848 |
amod |
Low,stringency |
R6741 |
T10848 |
T10850 |
compound |
stringency,conditions |
R6742 |
T10849 |
T10848 |
punct |
-,stringency |
R6743 |
T10850 |
T10852 |
nsubj |
conditions,were |
R6744 |
T10851 |
T10850 |
compound |
hybridization,conditions |
R6745 |
T10853 |
T10854 |
mark |
as,described |
R6746 |
T10854 |
T10852 |
advcl |
described,were |
R6747 |
T10855 |
T10856 |
punct |
[,1 |
R6748 |
T10856 |
T10854 |
parataxis |
1,described |
R6749 |
T10857 |
T10856 |
punct |
],1 |
R6750 |
T10858 |
T10852 |
punct |
.,were |
R6751 |
T10860 |
T10861 |
advmod |
Clonally,pure |
R6752 |
T10861 |
T10863 |
amod |
pure,plaques |
R6753 |
T10862 |
T10861 |
punct |
-,pure |
R6754 |
T10863 |
T10864 |
nsubjpass |
plaques,obtained |
R6755 |
T10865 |
T10864 |
auxpass |
were,obtained |
R6756 |
T10866 |
T10864 |
prep |
through,obtained |
R6757 |
T10867 |
T10868 |
amod |
secondary,screens |
R6758 |
T10868 |
T10866 |
pobj |
screens,through |
R6759 |
T10869 |
T10864 |
advcl |
using,obtained |
R6760 |
T10870 |
T10871 |
det |
the,probe |
R6761 |
T10871 |
T10869 |
dobj |
probe,using |
R6762 |
T10872 |
T10871 |
amod |
same,probe |
R6763 |
T10873 |
T10871 |
prep |
as,probe |
R6764 |
T10874 |
T10875 |
det |
the,screen |
R6765 |
T10875 |
T10873 |
pobj |
screen,as |
R6766 |
T10876 |
T10875 |
amod |
corresponding,screen |
R6767 |
T10877 |
T10875 |
amod |
primary,screen |
R6768 |
T10878 |
T10864 |
punct |
.,obtained |
R6769 |
T10880 |
T10881 |
nsubjpass |
PCR,performed |
R6770 |
T10882 |
T10880 |
prep |
with,PCR |
R6771 |
T10883 |
T10884 |
compound |
vector,primers |
R6772 |
T10884 |
T10882 |
pobj |
primers,with |
R6773 |
T10885 |
T10886 |
punct |
(,R |
R6774 |
T10886 |
T10880 |
parataxis |
R,PCR |
R6775 |
T10887 |
T10886 |
compound |
M13F,R |
R6776 |
T10888 |
T10886 |
punct |
/,R |
R6777 |
T10889 |
T10886 |
punct |
),R |
R6778 |
T10890 |
T10881 |
auxpass |
was,performed |
R6779 |
T10891 |
T10892 |
aux |
to,prepare |
R6780 |
T10892 |
T10881 |
advcl |
prepare,performed |
R6781 |
T10893 |
T10894 |
compound |
sequencing,templates |
R6782 |
T10894 |
T10892 |
dobj |
templates,prepare |
R6783 |
T10895 |
T10881 |
punct |
.,performed |
R6784 |
T10897 |
T10898 |
compound |
cDNA,size |
R6785 |
T10898 |
T10899 |
compound |
size,estimates |
R6786 |
T10899 |
T10900 |
nsubjpass |
estimates,obtained |
R6787 |
T10901 |
T10900 |
auxpass |
were,obtained |
R6788 |
T10902 |
T10900 |
prep |
by,obtained |
R6789 |
T10903 |
T10904 |
compound |
agarose,gel |
R6790 |
T10904 |
T10905 |
compound |
gel,electrophoresis |
R6791 |
T10905 |
T10902 |
pobj |
electrophoresis,by |
R6792 |
T10906 |
T10900 |
punct |
", ",obtained |
R6793 |
T10907 |
T10900 |
cc |
and,obtained |
R6794 |
T10908 |
T10909 |
nsubjpass |
inserts,sequenced |
R6795 |
T10909 |
T10900 |
conj |
sequenced,obtained |
R6796 |
T10910 |
T10909 |
auxpass |
were,sequenced |
R6797 |
T10911 |
T10909 |
prep |
from,sequenced |
R6798 |
T10912 |
T10913 |
det |
the,end |
R6799 |
T10913 |
T10911 |
pobj |
end,from |
R6800 |
T10914 |
T10913 |
nummod |
5,end |
R6801 |
T10915 |
T10914 |
punct |
',5 |
R6802 |
T10916 |
T10909 |
advcl |
using,sequenced |
R6803 |
T10917 |
T10918 |
det |
the,primer |
R6804 |
T10918 |
T10916 |
dobj |
primer,using |
R6805 |
T10919 |
T10918 |
compound |
M13R,primer |
R6806 |
T10920 |
T10918 |
cc |
and,primer |
R6807 |
T10921 |
T10922 |
amod |
big,dye |
R6808 |
T10922 |
T10924 |
compound |
dye,chemistry |
R6809 |
T10923 |
T10922 |
punct |
-,dye |
R6810 |
T10924 |
T10918 |
conj |
chemistry,primer |
R6811 |
T10925 |
T10924 |
compound |
terminator,chemistry |
R6812 |
T10926 |
T10916 |
prep |
according,using |
R6813 |
T10927 |
T10926 |
prep |
to,according |
R6814 |
T10928 |
T10929 |
poss |
ABI,protocols |
R6815 |
T10929 |
T10927 |
pobj |
protocols,to |
R6816 |
T10930 |
T10928 |
case |
's,ABI |
R6817 |
T10931 |
T10932 |
punct |
(,Biosystems |
R6818 |
T10932 |
T10929 |
parataxis |
Biosystems,protocols |
R6819 |
T10933 |
T10932 |
compound |
Applied,Biosystems |
R6820 |
T10934 |
T10932 |
punct |
", ",Biosystems |
R6821 |
T10935 |
T10936 |
compound |
Foster,City |
R6822 |
T10936 |
T10932 |
npadvmod |
City,Biosystems |
R6823 |
T10937 |
T10932 |
punct |
", ",Biosystems |
R6824 |
T10938 |
T10932 |
npadvmod |
CA,Biosystems |
R6825 |
T10939 |
T10932 |
punct |
", ",Biosystems |
R6826 |
T10940 |
T10932 |
npadvmod |
USA,Biosystems |
R6827 |
T10941 |
T10932 |
punct |
),Biosystems |
R6828 |
T10942 |
T10909 |
punct |
.,sequenced |
R6829 |
T10944 |
T10945 |
prep |
In,converted |
R6830 |
T10946 |
T10944 |
pobj |
order,In |
R6831 |
T10947 |
T10948 |
aux |
to,obtain |
R6832 |
T10948 |
T10946 |
acl |
obtain,order |
R6833 |
T10949 |
T10950 |
nummod |
3,sequence |
R6834 |
T10950 |
T10948 |
dobj |
sequence,obtain |
R6835 |
T10951 |
T10949 |
punct |
',3 |
R6836 |
T10952 |
T10945 |
punct |
", ",converted |
R6837 |
T10953 |
T10954 |
amod |
selected,clones |
R6838 |
T10954 |
T10945 |
nsubjpass |
clones,converted |
R6839 |
T10955 |
T10954 |
compound |
phage,clones |
R6840 |
T10956 |
T10945 |
auxpass |
were,converted |
R6841 |
T10957 |
T10945 |
prep |
to,converted |
R6842 |
T10958 |
T10959 |
compound |
plasmid,stocks |
R6843 |
T10959 |
T10957 |
pobj |
stocks,to |
R6844 |
T10960 |
T10945 |
prep |
following,converted |
R6845 |
T10961 |
T10962 |
det |
a,version |
R6846 |
T10962 |
T10960 |
pobj |
version,following |
R6847 |
T10963 |
T10962 |
amod |
scaled,version |
R6848 |
T10964 |
T10963 |
punct |
-,scaled |
R6849 |
T10965 |
T10963 |
prt |
down,scaled |
R6850 |
T10966 |
T10962 |
prep |
of,version |
R6851 |
T10967 |
T10968 |
poss |
Stratagene,protocol |
R6852 |
T10968 |
T10966 |
pobj |
protocol,of |
R6853 |
T10969 |
T10967 |
case |
's,Stratagene |
R6854 |
T10970 |
T10971 |
advmod |
in,vivo |
R6855 |
T10971 |
T10968 |
amod |
vivo,protocol |
R6856 |
T10972 |
T10968 |
compound |
excision,protocol |
R6857 |
T10973 |
T10945 |
punct |
.,converted |
R6858 |
T10975 |
T10976 |
compound |
Plasmid,DNA |
R6859 |
T10976 |
T10977 |
nsubj |
DNA,gave |
R6860 |
T10978 |
T10979 |
amod |
better,sequence |
R6861 |
T10979 |
T10977 |
dobj |
sequence,gave |
R6862 |
T10980 |
T10981 |
nummod |
3,end |
R6863 |
T10981 |
T10979 |
compound |
end,sequence |
R6864 |
T10982 |
T10980 |
punct |
',3 |
R6865 |
T10983 |
T10981 |
punct |
-,end |
R6866 |
T10984 |
T10979 |
prep |
than,sequence |
R6867 |
T10985 |
T10986 |
compound |
PCR,products |
R6868 |
T10986 |
T10984 |
pobj |
products,than |
R6869 |
T10987 |
T10986 |
punct |
", ",products |
R6870 |
T10988 |
T10989 |
dep |
which,suffered |
R6871 |
T10989 |
T10986 |
relcl |
suffered,products |
R6872 |
T10990 |
T10989 |
advmod |
often,suffered |
R6873 |
T10991 |
T10989 |
prep |
from,suffered |
R6874 |
T10992 |
T10991 |
pobj |
polymerase,from |
R6875 |
T10993 |
T10992 |
amod |
stuttering,polymerase |
R6876 |
T10994 |
T10989 |
prep |
through,suffered |
R6877 |
T10995 |
T10996 |
det |
the,tract |
R6878 |
T10996 |
T10994 |
pobj |
tract,through |
R6879 |
T10997 |
T10996 |
nmod |
poly,tract |
R6880 |
T10998 |
T10997 |
punct |
(,poly |
R6881 |
T10999 |
T10997 |
appos |
A,poly |
R6882 |
T11000 |
T10996 |
punct |
),tract |
R6883 |
T11001 |
T10977 |
punct |
.,gave |