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{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T5682","span":{"begin":21,"end":30},"obj":"GO:0007608"},{"id":"T5683","span":{"begin":21,"end":40},"obj":"GO_EXT:0004984"},{"id":"T5684","span":{"begin":60,"end":64},"obj":"SO_EXT:cDNA"},{"id":"T5685","span":{"begin":98,"end":113},"obj":"GO:0043631"},{"id":"T5686","span":{"begin":98,"end":118},"obj":"SO_EXT:0000553"},{"id":"T5687","span":{"begin":145,"end":151},"obj":"SO_EXT:0000205"},{"id":"T5688","span":{"begin":152,"end":160},"obj":"SO_EXT:0001060"},{"id":"T5689","span":{"begin":188,"end":194},"obj":"SO_EXT:0000205"},{"id":"T5690","span":{"begin":210,"end":221},"obj":"SO_EXT:0000317"},{"id":"T5691","span":{"begin":247,"end":253},"obj":"SO_EXT:0000667"},{"id":"T5692","span":{"begin":279,"end":287},"obj":"SO_EXT:biological_sequence"},{"id":"T5693","span":{"begin":308,"end":314},"obj":"SO_EXT:0000205"},{"id":"T5694","span":{"begin":315,"end":322},"obj":"SO_EXT:0001060"},{"id":"T5695","span":{"begin":359,"end":364},"obj":"SO_EXT:0000704"},{"id":"T5696","span":{"begin":399,"end":404},"obj":"SO_EXT:cDNA"},{"id":"T5697","span":{"begin":410,"end":416},"obj":"SO_EXT:0000205"},{"id":"T5698","span":{"begin":464,"end":479},"obj":"GO:0043631"},{"id":"T5699","span":{"begin":480,"end":488},"obj":"SO_EXT:0001060"},{"id":"T5700","span":{"begin":518,"end":523},"obj":"SO_EXT:0000704"},{"id":"T5701","span":{"begin":555,"end":566},"obj":"SO_EXT:0000317"},{"id":"T5702","span":{"begin":577,"end":586},"obj":"SO_EXT:biological_sequence"},{"id":"T5703","span":{"begin":605,"end":609},"obj":"SO_EXT:cDNA"},{"id":"T5704","span":{"begin":620,"end":632},"obj":"SO_EXT:0000610"},{"id":"T5705","span":{"begin":625,"end":626},"obj":"CHEBI_EXT:adenine_nucleobase_or_nucleoside_or_nucleotide_molecular_entity_or_group"},{"id":"T5706","span":{"begin":646,"end":648},"obj":"SO_EXT:0000028"},{"id":"T5707","span":{"begin":660,"end":670},"obj":"SO_EXT:0000319"},{"id":"T5708","span":{"begin":693,"end":699},"obj":"SO_EXT:0000188"},{"id":"T5709","span":{"begin":704,"end":711},"obj":"GO:0008380"},{"id":"T5710","span":{"begin":723,"end":729},"obj":"SO_EXT:0000205"},{"id":"T5711","span":{"begin":774,"end":783},"obj":"GO:0007608"},{"id":"T5712","span":{"begin":774,"end":792},"obj":"GO_EXT:0004984"},{"id":"T5713","span":{"begin":793,"end":797},"obj":"SO_EXT:0000704"}],"text":"At least half of the olfactory receptors represented in our cDNA collection utilize more than one polyadenylation site, resulting in alternative 3' UTR isoforms. We have crudely estimated 3' UTR size for 1,169 cDNA clones by combining approximate insert size information with 5' sequence data. More than one 3' UTR isoform is predicted for 43 of the 77 (56%) genes for which there are at least four cDNAs with 3' UTR size information. We confirmed the alternative polyadenylation isoforms of four out of five selected genes by sequencing the 3' end of 14 cDNA clones. These 14 sequences also revealed one cDNA where the poly(A) tail was added 27 bp before the stop codon, and another where an intron was spliced out of the 3' UTR, contrary to the conventional stereotype of olfactory receptor gene structure."}

{"project":"craft-ca-core-dev","denotations":[{"id":"T5628","span":{"begin":21,"end":30},"obj":"GO:0007608"},{"id":"T5629","span":{"begin":98,"end":113},"obj":"GO:0043631"},{"id":"T5630","span":{"begin":98,"end":118},"obj":"SO:0000553"},{"id":"T5631","span":{"begin":145,"end":151},"obj":"SO:0000205"},{"id":"T5632","span":{"begin":152,"end":160},"obj":"SO:0001060"},{"id":"T5633","span":{"begin":188,"end":194},"obj":"SO:0000205"},{"id":"T5634","span":{"begin":210,"end":221},"obj":"SO:0000317"},{"id":"T5635","span":{"begin":247,"end":253},"obj":"SO:0000667"},{"id":"T5636","span":{"begin":308,"end":314},"obj":"SO:0000205"},{"id":"T5637","span":{"begin":315,"end":322},"obj":"SO:0001060"},{"id":"T5638","span":{"begin":359,"end":364},"obj":"SO:0000704"},{"id":"T5639","span":{"begin":410,"end":416},"obj":"SO:0000205"},{"id":"T5640","span":{"begin":464,"end":479},"obj":"GO:0043631"},{"id":"T5641","span":{"begin":480,"end":488},"obj":"SO:0001060"},{"id":"T5642","span":{"begin":518,"end":523},"obj":"SO:0000704"},{"id":"T5643","span":{"begin":555,"end":566},"obj":"SO:0000317"},{"id":"T5644","span":{"begin":620,"end":632},"obj":"SO:0000610"},{"id":"T5645","span":{"begin":646,"end":648},"obj":"SO:0000028"},{"id":"T5646","span":{"begin":660,"end":670},"obj":"SO:0000319"},{"id":"T5647","span":{"begin":693,"end":699},"obj":"SO:0000188"},{"id":"T5648","span":{"begin":704,"end":711},"obj":"GO:0008380"},{"id":"T5649","span":{"begin":723,"end":729},"obj":"SO:0000205"},{"id":"T5650","span":{"begin":774,"end":783},"obj":"GO:0007608"},{"id":"T5651","span":{"begin":793,"end":797},"obj":"SO:0000704"}],"text":"At least half of the olfactory receptors represented in our cDNA collection utilize more than one polyadenylation site, resulting in alternative 3' UTR isoforms. We have crudely estimated 3' UTR size for 1,169 cDNA clones by combining approximate insert size information with 5' sequence data. More than one 3' UTR isoform is predicted for 43 of the 77 (56%) genes for which there are at least four cDNAs with 3' UTR size information. We confirmed the alternative polyadenylation isoforms of four out of five selected genes by sequencing the 3' end of 14 cDNA clones. These 14 sequences also revealed one cDNA where the poly(A) tail was added 27 bp before the stop codon, and another where an intron was spliced out of the 3' UTR, contrary to the conventional stereotype of olfactory receptor gene structure."}