PMC:3279418 / 7016-7114 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T13165	0-5	IN	denotes	After
T13166	6-8	CD	denotes	48
T13167	9-14	NN	denotes	hours
T13168	14-15	-COMMA-	denotes	,
T13169	16-21	NN	denotes	cells
T13170	22-26	VB	denotes	were
T13171	27-32	VB	denotes	fixed
T13172	33-36	CC	denotes	and
T13173	37-43	VB	denotes	probed
T13174	44-48	IN	denotes	with
T13175	49-58	JJ	denotes	anti-FLAG
T13176	59-62	CC	denotes	and
T13177	63-78	JJ	denotes	FITC-conjugated
T13178	79-88	JJ	denotes	secondary
T13179	89-97	NN	denotes	antibody
R10958	T13167	T13165	arg2Of	hours,After
R10959	T13167	T13166	arg1Of	hours,48
R10960	T13169	T13170	arg1Of	cells,were
R10961	T13169	T13171	arg2Of	cells,fixed
R10962	T13169	T13173	arg2Of	cells,probed
R10963	T13171	T13172	arg1Of	fixed,and
R10964	T13172	T13165	arg1Of	and,After
R10965	T13172	T13168	arg1Of	and,","
R10966	T13172	T13170	arg2Of	and,were
R10967	T13173	T13172	arg2Of	probed,and
R10968	T13173	T13174	arg1Of	probed,with
R10969	T13175	T13176	arg1Of	anti-FLAG,and
R10970	T13177	T13176	arg2Of	FITC-conjugated,and
R10971	T13179	T13174	arg2Of	antibody,with
R10972	T13179	T13175	arg1Of	antibody,anti-FLAG
R10973	T13179	T13177	arg1Of	antibody,FITC-conjugated
R10974	T13179	T13178	arg1Of	antibody,secondary

Id	Subject	Object	Predicate	Lexical cue
T12956	0-5	IN	denotes	After
T12957	6-8	CD	denotes	48
T12958	9-14	NNS	denotes	hours
T12959	14-15	,	denotes	,
T12960	16-21	NNS	denotes	cells
T12961	22-26	VBD	denotes	were
T12962	27-32	VBN	denotes	fixed
T12963	33-36	CC	denotes	and
T12964	37-43	VBN	denotes	probed
T12965	44-48	IN	denotes	with
T12966	49-58	JJ	denotes	anti-FLAG
T12967	59-62	CC	denotes	and
T12968	63-78	JJ	denotes	FITC-conjugated
T12969	79-88	JJ	denotes	secondary
T12970	89-97	NN	denotes	antibody
T12971	97-98	.	denotes	.
R10743	T12956	T12962	prep	After,fixed
R10744	T12957	T12958	nummod	48,hours
R10745	T12958	T12956	pobj	hours,After
R10746	T12959	T12962	punct	",",fixed
R10747	T12960	T12962	nsubjpass	cells,fixed
R10748	T12961	T12962	auxpass	were,fixed
R10749	T12962	T12962	ROOT	fixed,fixed
R10750	T12963	T12962	cc	and,fixed
R10751	T12964	T12962	conj	probed,fixed
R10752	T12965	T12964	prep	with,probed
R10753	T12966	T12970	amod	anti-FLAG,antibody
R10754	T12967	T12966	cc	and,anti-FLAG
R10755	T12968	T12966	conj	FITC-conjugated,anti-FLAG
R10756	T12969	T12970	amod	secondary,antibody
R10757	T12970	T12965	pobj	antibody,with
R10758	T12971	T12962	punct	.,fixed

Id	Subject	Object	Predicate	Lexical cue
T13215	89-97	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody

Id	Subject	Object	Predicate	Lexical cue
T13217	16-21	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T13219	89-97	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T13220	89-97	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody

Id	Subject	Object	Predicate	Lexical cue
T12768	0-98	Sentence	denotes	After 48 hours, cells were fixed and probed with anti-FLAG and FITC-conjugated secondary antibody.
T46	0-98	Sentence	denotes	After 48 hours, cells were fixed and probed with anti-FLAG and FITC-conjugated secondary antibody.