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PMC:3279418 / 28265-29509 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T10676 0-9 NN denotes Phenotype
T10677 10-12 IN denotes of
T10678 13-17 NN denotes BMDC
T10679 18-21 CC denotes and
T10680 22-29 JJ denotes splenic
T10681 30-32 NN denotes DC
T10682 33-40 NN denotes subsets
T10683 41-44 DT denotes The
T10684 45-54 NN denotes phenotype
T10685 55-57 IN denotes of
T10686 58-62 NN denotes BMDC
T10687 63-66 VB denotes was
T10688 67-77 VB denotes determined
T10689 78-84 IN denotes before
T10690 85-88 CC denotes and
T10691 89-94 IN denotes after
T10692 95-97 CD denotes 24
T10693 98-102 NN denotes hour
T10694 103-110 NN denotes culture
T10695 111-113 IN denotes in
T10696 114-117 DT denotes the
T10697 118-126 NN denotes presence
T10698 127-129 IN denotes of
T10699 130-133 CD denotes 100
T10700 134-139 NN denotes ng/mL
T10701 140-143 NN denotes LPS
T10702 145-148 DT denotes The
T10703 149-154 NN denotes cells
T10704 155-159 VB denotes were
T10705 160-169 VB denotes incubated
T10706 170-172 IN denotes in
T10707 173-176 NNP denotes PBS
T10708 177-181 IN denotes with
T10709 182-185 CD denotes 0.1
T10710 185-186 NN denotes %
T10711 187-191 NN denotes NaN3
T10712 191-192 -COMMA- denotes ,
T10713 193-194 CD denotes 1
T10714 194-195 NN denotes %
T10715 196-199 NN denotes BSA
T10716 200-203 CC denotes and
T10717 204-206 CD denotes 10
T10718 206-207 NN denotes %
T10719 208-214 JJ denotes normal
T10720 215-221 NN denotes rabbit
T10721 222-227 NN denotes serum
T10722 228-231 IN denotes for
T10723 232-234 CD denotes 20
T10724 235-242 NN denotes minutes
T10725 243-245 IN denotes on
T10726 246-249 NN denotes ice
T10727 249-250 -COMMA- denotes ,
T10728 251-257 VB denotes washed
T10729 258-261 CC denotes and
T10730 262-271 VB denotes incubated
T10731 272-275 IN denotes for
T10732 276-278 CD denotes 30
T10733 279-286 NN denotes minutes
T10734 287-289 IN denotes on
T10735 290-293 NN denotes ice
T10736 294-298 IN denotes with
T10737 299-303 NNP denotes Alex
T10738 304-317 JJ denotes Fluor-labeled
T10739 318-328 NN denotes anti-CD11c
T10740 329-330 -LRB- denotes (
T10741 330-338 NN denotes MCD11C20
T10742 338-339 -COMMA- denotes ,
T10743 340-346 NN denotes CALTAG
T10744 346-347 -RRB- denotes )
T10745 348-350 IN denotes in
T10746 351-362 NN denotes combination
T10747 363-367 IN denotes with
T10748 368-371 NN denotes PE-
T10749 372-381 NN denotes anti-CD40
T10750 382-383 -LRB- denotes (
T10751 383-387 CD denotes 3/23
T10752 387-388 -COMMA- denotes ,
T10753 389-391 NNP denotes BD
T10754 392-403 NNP denotes Biosciences
T10755 403-404 -RRB- denotes )
T10756 404-405 -COMMA- denotes ,
T10757 406-418 NN denotes PE-anti-CD80
T10758 419-420 -LRB- denotes (
T10759 420-427 NN denotes 16-10A1
T10760 427-428 -COMMA- denotes ,
T10761 429-440 NN denotes eBioscience
T10762 440-441 -RRB- denotes )
T10763 441-442 -COMMA- denotes ,
T10764 443-455 NN denotes PE-anti-CD86
T10765 456-457 -LRB- denotes (
T10766 457-462 NN denotes P03.1
T10767 462-463 -COMMA- denotes ,
T10768 464-475 NN denotes eBioscience
T10769 475-476 -RRB- denotes )
T10770 476-477 -COMMA- denotes ,
T10771 478-490 NN denotes PE-anti-CD14
T10772 491-492 -LRB- denotes (
T10773 492-497 NN denotes Sa2.8
T10774 497-498 -COMMA- denotes ,
T10775 499-510 NN denotes eBioscience
T10776 510-511 -RRB- denotes )
T10777 512-515 CC denotes and
T10778 516-528 VB denotes biotinylated
T10779 529-538 NN denotes anti-TLR4
T10780 539-540 -LRB- denotes (
T10781 540-549 NN denotes BioLegend
T10782 549-550 -RRB- denotes )
T10783 552-555 DT denotes The
T10784 556-561 NN denotes cells
T10785 562-571 VB denotes incubated
T10786 572-576 IN denotes with
T10787 577-589 VB denotes biotinylated
T10788 590-604 NN denotes anti-TLR4/MD-2
T10789 605-609 VB denotes were
T10790 610-616 VB denotes washed
T10791 617-622 RB denotes twice
T10792 623-626 CC denotes and
T10793 627-636 VB denotes incubated
T10794 637-640 IN denotes for
T10795 641-643 CD denotes 30
T10796 644-651 NN denotes minutes
T10797 652-654 IN denotes on
T10798 655-658 NN denotes ice
T10799 659-663 IN denotes with
T10800 664-673 NN denotes avidin-PE
T10801 675-677 TO denotes To
T10802 678-685 VB denotes isolate
T10803 686-693 JJ denotes splenic
T10804 694-696 NN denotes DC
T10805 696-697 -COMMA- denotes ,
T10806 698-705 NN denotes spleens
T10807 706-710 VB denotes were
T10808 711-722 NN denotes collagenase
T10809 723-731 VB denotes digested
T10810 732-735 CC denotes and
T10811 736-743 JJ denotes subject
T10812 744-746 TO denotes to
T10813 747-754 VB denotes Percoll
T10814 755-763 NN denotes gradient
T10815 764-778 NN denotes centrifugation
T10816 780-783 DT denotes The
T10817 784-789 NN denotes bands
T10818 790-792 IN denotes at
T10819 793-796 DT denotes the
T10820 797-802 CD denotes 35–55
T10821 802-803 NN denotes %
T10822 804-813 NN denotes interface
T10823 814-818 VB denotes were
T10824 819-828 VB denotes collected
T10825 829-831 TO denotes to
T10826 832-837 VB denotes stain
T10827 838-842 IN denotes with
T10828 843-856 NN denotes PE-anti-CD11c
T10829 857-858 -LRB- denotes (
T10830 858-861 NN denotes HL3
T10831 861-862 -COMMA- denotes ,
T10832 863-872 NNP denotes BioLegend
T10833 872-873 -RRB- denotes )
T10834 873-874 -COMMA- denotes ,
T10835 875-890 NN denotes APC-anti-PDCA-1
T10836 891-892 -LRB- denotes (
T10837 892-895 CD denotes 927
T10838 895-896 -COMMA- denotes ,
T10839 897-906 NNP denotes BioLegend
T10840 906-907 -RRB- denotes )
T10841 908-911 CC denotes and
T10842 912-926 NN denotes FITC-anti-CD8α
T10843 927-928 -LRB- denotes (
T10844 928-934 CD denotes 53–6.7
T10845 934-935 -COMMA- denotes ,
T10846 936-938 NNP denotes BD
T10847 939-949 NNP denotes BioScience
T10848 949-950 -RRB- denotes )
T10849 952-955 DT denotes The
T10850 956-961 NN denotes cells
T10851 962-966 VB denotes were
T10852 967-973 VB denotes washed
T10853 974-979 RB denotes twice
T10854 979-980 -COMMA- denotes ,
T10855 981-986 VB denotes fixed
T10856 987-989 IN denotes in
T10857 990-991 CD denotes 2
T10858 991-992 NN denotes %
T10859 993-1009 NN denotes paraformaldehyde
T10860 1009-1010 -COMMA- denotes ,
T10861 1011-1014 CC denotes and
T10862 1015-1021 VB denotes stored
T10863 1022-1024 IN denotes at
T10864 1025-1028 NN denotes 4°C
T10865 1029-1034 IN denotes until
T10866 1035-1043 NN denotes analysis
T10867 1045-1049 NN denotes Flow
T10868 1050-1059 NN denotes cytometry
T10869 1060-1063 VB denotes was
T10870 1064-1073 VB denotes performed
T10871 1074-1076 IN denotes in
T10872 1077-1079 DT denotes an
T10873 1080-1085 NNP denotes Excel
T10874 1086-1087 -LRB- denotes (
T10875 1087-1094 NNP denotes Coulter
T10876 1094-1095 -RRB- denotes )
T10877 1096-1106 NN denotes instrument
T10878 1108-1112 NN denotes Dead
T10879 1113-1118 NN denotes cells
T10880 1119-1123 VB denotes were
T10881 1124-1131 VB denotes omitted
T10882 1132-1136 IN denotes from
T10883 1137-1140 DT denotes the
T10884 1141-1149 NN denotes analysis
T10885 1150-1152 IN denotes by
T10886 1153-1159 VB denotes gating
T10887 1160-1162 IN denotes on
T10888 1163-1170 JJ denotes forward
T10889 1171-1174 CC denotes and
T10890 1175-1178 CD denotes 90°
T10891 1179-1184 JJ denotes light
T10892 1185-1192 NN denotes scatter
T10893 1192-1193 -COMMA- denotes ,
T10894 1194-1197 CC denotes and
T10895 1198-1204 CD denotes 10,000
T10896 1205-1210 NN denotes cells
T10897 1211-1215 VB denotes were
T10898 1216-1224 VB denotes analyzed
T10899 1225-1227 IN denotes by
T10900 1228-1234 NNP denotes FlowJo
T10901 1235-1243 NN denotes software
R8828 T10676 T10677 arg1Of Phenotype,of
R8829 T10678 T10679 arg1Of BMDC,and
R8830 T10680 T10679 arg2Of splenic,and
R8831 T10682 T10677 arg2Of subsets,of
R8832 T10682 T10678 arg1Of subsets,BMDC
R8833 T10682 T10680 arg1Of subsets,splenic
R8834 T10682 T10681 arg1Of subsets,DC
R8835 T10684 T10683 arg1Of phenotype,The
R8836 T10684 T10685 arg1Of phenotype,of
R8837 T10684 T10687 arg1Of phenotype,was
R8838 T10684 T10688 arg2Of phenotype,determined
R8839 T10686 T10685 arg2Of BMDC,of
R8840 T10688 T10687 arg2Of determined,was
R8841 T10688 T10689 arg1Of determined,before
R8842 T10688 T10691 arg1Of determined,after
R8843 T10688 T10695 arg1Of determined,in
R8844 T10689 T10690 arg1Of before,and
R8845 T10691 T10690 arg2Of after,and
R8846 T10692 T10693 arg1Of 24,hour
R8847 T10694 T10689 arg2Of culture,before
R8848 T10694 T10691 arg2Of culture,after
R8849 T10694 T10692 arg1Of culture,24
R8850 T10695 T10696 arg1Of in,the
R8851 T10695 T10698 arg1Of in,of
R8852 T10697 T10695 arg2Of presence,in
R8853 T10699 T10700 arg1Of 100,ng/mL
R8854 T10701 T10695 arg3Of LPS,in
R8855 T10701 T10699 arg1Of LPS,100
R8856 T10703 T10702 arg1Of cells,The
R8857 T10703 T10704 arg1Of cells,were
R8858 T10703 T10705 arg2Of cells,incubated
R8859 T10703 T10730 arg2Of cells,incubated
R8860 T10705 T10706 arg1Of incubated,in
R8861 T10705 T10708 arg1Of incubated,with
R8862 T10705 T10729 arg1Of incubated,and
R8863 T10707 T10706 arg2Of PBS,in
R8864 T10709 T10710 arg1Of 0.1,%
R8865 T10711 T10709 arg1Of NaN3,0.1
R8866 T10711 T10712 arg1Of NaN3,","
R8867 T10712 T10716 arg1Of ",",and
R8868 T10713 T10714 arg1Of 1,%
R8869 T10715 T10712 arg2Of BSA,","
R8870 T10715 T10713 arg1Of BSA,1
R8871 T10716 T10708 arg2Of and,with
R8872 T10716 T10727 arg1Of and,","
R8873 T10716 T10728 arg2Of and,washed
R8874 T10717 T10718 arg1Of 10,%
R8875 T10721 T10716 arg2Of serum,and
R8876 T10721 T10717 arg1Of serum,10
R8877 T10721 T10719 arg1Of serum,normal
R8878 T10721 T10720 arg1Of serum,rabbit
R8879 T10721 T10722 arg1Of serum,for
R8880 T10721 T10725 arg1Of serum,on
R8881 T10724 T10722 arg2Of minutes,for
R8882 T10724 T10723 arg1Of minutes,20
R8883 T10726 T10725 arg2Of ice,on
R8884 T10729 T10704 arg2Of and,were
R8885 T10730 T10729 arg2Of incubated,and
R8886 T10730 T10731 arg1Of incubated,for
R8887 T10730 T10734 arg1Of incubated,on
R8888 T10733 T10731 arg2Of minutes,for
R8889 T10733 T10732 arg1Of minutes,30
R8890 T10735 T10734 arg2Of ice,on
R8891 T10735 T10736 arg1Of ice,with
R8892 T10739 T10736 arg2Of anti-CD11c,with
R8893 T10739 T10737 arg1Of anti-CD11c,Alex
R8894 T10739 T10738 arg1Of anti-CD11c,Fluor-labeled
R8895 T10739 T10740 arg1Of anti-CD11c,(
R8896 T10739 T10745 arg1Of anti-CD11c,in
R8897 T10739 T10750 arg1Of anti-CD11c,(
R8898 T10739 T10756 arg1Of anti-CD11c,","
R8899 T10741 T10740 arg2Of MCD11C20,(
R8900 T10741 T10742 arg1Of MCD11C20,","
R8901 T10743 T10742 arg2Of CALTAG,","
R8902 T10744 T10740 arg3Of ),(
R8903 T10746 T10745 arg2Of combination,in
R8904 T10746 T10747 arg1Of combination,with
R8905 T10749 T10747 arg2Of anti-CD40,with
R8906 T10749 T10748 arg1Of anti-CD40,PE-
R8907 T10751 T10750 arg2Of 3/23,(
R8908 T10751 T10752 arg1Of 3/23,","
R8909 T10754 T10752 arg2Of Biosciences,","
R8910 T10754 T10753 arg1Of Biosciences,BD
R8911 T10755 T10750 arg3Of ),(
R8912 T10757 T10758 arg1Of PE-anti-CD80,(
R8913 T10757 T10763 arg1Of PE-anti-CD80,","
R8914 T10759 T10758 arg2Of 16-10A1,(
R8915 T10759 T10760 arg1Of 16-10A1,","
R8916 T10761 T10760 arg2Of eBioscience,","
R8917 T10762 T10758 arg3Of ),(
R8918 T10763 T10765 arg1Of ",",(
R8919 T10763 T10770 arg1Of ",",","
R8920 T10764 T10763 arg2Of PE-anti-CD86,","
R8921 T10766 T10765 arg2Of P03.1,(
R8922 T10766 T10767 arg1Of P03.1,","
R8923 T10768 T10767 arg2Of eBioscience,","
R8924 T10769 T10765 arg3Of ),(
R8925 T10770 T10756 arg2Of ",",","
R8926 T10771 T10772 arg1Of PE-anti-CD14,(
R8927 T10771 T10777 arg1Of PE-anti-CD14,and
R8928 T10773 T10772 arg2Of Sa2.8,(
R8929 T10773 T10774 arg1Of Sa2.8,","
R8930 T10775 T10774 arg2Of eBioscience,","
R8931 T10776 T10772 arg3Of ),(
R8932 T10777 T10770 arg2Of and,","
R8933 T10779 T10777 arg2Of anti-TLR4,and
R8934 T10779 T10778 arg2Of anti-TLR4,biotinylated
R8935 T10779 T10780 arg1Of anti-TLR4,(
R8936 T10781 T10780 arg2Of BioLegend,(
R8937 T10782 T10780 arg3Of ),(
R8938 T10784 T10783 arg1Of cells,The
R8939 T10784 T10785 arg2Of cells,incubated
R8940 T10784 T10789 arg1Of cells,were
R8941 T10784 T10790 arg2Of cells,washed
R8942 T10784 T10793 arg2Of cells,incubated
R8943 T10785 T10786 arg1Of incubated,with
R8944 T10788 T10786 arg2Of anti-TLR4/MD-2,with
R8945 T10788 T10787 arg2Of anti-TLR4/MD-2,biotinylated
R8946 T10790 T10791 arg1Of washed,twice
R8947 T10790 T10792 arg1Of washed,and
R8948 T10792 T10789 arg2Of and,were
R8949 T10793 T10792 arg2Of incubated,and
R8950 T10793 T10794 arg1Of incubated,for
R8951 T10796 T10794 arg2Of minutes,for
R8952 T10796 T10795 arg1Of minutes,30
R8953 T10796 T10797 arg1Of minutes,on
R8954 T10798 T10797 arg2Of ice,on
R8955 T10798 T10799 arg1Of ice,with
R8956 T10800 T10799 arg2Of avidin-PE,with
R8957 T10802 T10801 arg1Of isolate,To
R8958 T10804 T10802 arg2Of DC,isolate
R8959 T10804 T10803 arg1Of DC,splenic
R8960 T10806 T10802 arg1Of spleens,isolate
R8961 T10806 T10807 arg1Of spleens,were
R8962 T10806 T10809 arg1Of spleens,digested
R8963 T10806 T10811 arg1Of spleens,subject
R8964 T10807 T10801 modOf were,To
R8965 T10807 T10805 arg1Of were,","
R8966 T10809 T10808 arg1Of digested,collagenase
R8967 T10809 T10810 arg1Of digested,and
R8968 T10810 T10807 arg2Of and,were
R8969 T10811 T10810 arg2Of subject,and
R8970 T10811 T10812 arg1Of subject,to
R8971 T10815 T10812 arg2Of centrifugation,to
R8972 T10815 T10813 arg1Of centrifugation,Percoll
R8973 T10815 T10814 arg1Of centrifugation,gradient
R8974 T10817 T10816 arg1Of bands,The
R8975 T10817 T10818 arg1Of bands,at
R8976 T10817 T10823 arg1Of bands,were
R8977 T10817 T10824 arg2Of bands,collected
R8978 T10820 T10821 arg1Of 35–55,%
R8979 T10822 T10818 arg2Of interface,at
R8980 T10822 T10819 arg1Of interface,the
R8981 T10822 T10820 arg1Of interface,35–55
R8982 T10824 T10823 arg2Of collected,were
R8983 T10824 T10825 modOf collected,to
R8984 T10826 T10825 arg1Of stain,to
R8985 T10826 T10827 arg1Of stain,with
R8986 T10828 T10829 arg1Of PE-anti-CD11c,(
R8987 T10828 T10834 arg1Of PE-anti-CD11c,","
R8988 T10830 T10829 arg2Of HL3,(
R8989 T10830 T10831 arg1Of HL3,","
R8990 T10830 T10832 arg1Of HL3,BioLegend
R8991 T10833 T10829 arg3Of ),(
R8992 T10834 T10836 arg1Of ",",(
R8993 T10834 T10841 arg1Of ",",and
R8994 T10835 T10834 arg2Of APC-anti-PDCA-1,","
R8995 T10837 T10836 arg2Of 927,(
R8996 T10837 T10838 arg1Of 927,","
R8997 T10839 T10838 arg2Of BioLegend,","
R8998 T10840 T10836 arg3Of ),(
R8999 T10841 T10827 arg2Of and,with
R9000 T10842 T10841 arg2Of FITC-anti-CD8α,and
R9001 T10842 T10843 arg1Of FITC-anti-CD8α,(
R9002 T10844 T10843 arg2Of 53–6.7,(
R9003 T10844 T10845 arg1Of 53–6.7,","
R9004 T10847 T10845 arg2Of BioScience,","
R9005 T10847 T10846 arg1Of BioScience,BD
R9006 T10848 T10843 arg3Of ),(
R9007 T10850 T10849 arg1Of cells,The
R9008 T10850 T10851 arg1Of cells,were
R9009 T10850 T10852 arg2Of cells,washed
R9010 T10850 T10855 arg2Of cells,fixed
R9011 T10850 T10862 arg1Of cells,stored
R9012 T10852 T10853 arg1Of washed,twice
R9013 T10852 T10854 arg1Of washed,","
R9014 T10854 T10851 arg2Of ",",were
R9015 T10854 T10861 arg1Of ",",and
R9016 T10855 T10854 arg2Of fixed,","
R9017 T10855 T10856 arg1Of fixed,in
R9018 T10857 T10858 arg1Of 2,%
R9019 T10859 T10856 arg2Of paraformaldehyde,in
R9020 T10859 T10857 arg1Of paraformaldehyde,2
R9021 T10861 T10860 arg1Of and,","
R9022 T10862 T10861 arg2Of stored,and
R9023 T10862 T10863 arg1Of stored,at
R9024 T10862 T10865 arg1Of stored,until
R9025 T10864 T10863 arg2Of 4°C,at
R9026 T10866 T10865 arg2Of analysis,until
R9027 T10868 T10867 arg1Of cytometry,Flow
R9028 T10868 T10869 arg1Of cytometry,was
R9029 T10868 T10870 arg2Of cytometry,performed
R9030 T10870 T10869 arg2Of performed,was
R9031 T10870 T10871 arg1Of performed,in
R9032 T10875 T10874 arg2Of Coulter,(
R9033 T10876 T10874 arg3Of ),(
R9034 T10877 T10871 arg2Of instrument,in
R9035 T10877 T10872 arg1Of instrument,an
R9036 T10877 T10873 arg1Of instrument,Excel
R9037 T10877 T10874 arg1Of instrument,(
R9038 T10879 T10878 arg1Of cells,Dead
R9039 T10879 T10880 arg1Of cells,were
R9040 T10879 T10881 arg2Of cells,omitted
R9041 T10879 T10886 arg1Of cells,gating
R9042 T10881 T10880 arg2Of omitted,were
R9043 T10881 T10882 arg1Of omitted,from
R9044 T10881 T10885 arg1Of omitted,by
R9045 T10881 T10894 arg1Of omitted,and
R9046 T10884 T10882 arg2Of analysis,from
R9047 T10884 T10883 arg1Of analysis,the
R9048 T10886 T10885 arg2Of gating,by
R9049 T10886 T10887 arg1Of gating,on
R9050 T10888 T10889 arg1Of forward,and
R9051 T10889 T10891 arg1Of and,light
R9052 T10890 T10889 arg2Of 90°,and
R9053 T10892 T10887 arg2Of scatter,on
R9054 T10892 T10888 arg1Of scatter,forward
R9055 T10892 T10890 arg1Of scatter,90°
R9056 T10894 T10893 arg1Of and,","
R9057 T10896 T10895 arg1Of cells,"10,000"
R9058 T10896 T10897 arg1Of cells,were
R9059 T10896 T10898 arg2Of cells,analyzed
R9060 T10898 T10894 arg2Of analyzed,and
R9061 T10898 T10897 arg2Of analyzed,were
R9062 T10901 T10898 arg1Of software,analyzed
R9063 T10901 T10899 arg2Of software,by
R9064 T10901 T10900 arg1Of software,FlowJo

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T10432 0-9 NNP denotes Phenotype
T10433 10-12 IN denotes of
T10434 13-17 NNP denotes BMDC
T10435 18-21 CC denotes and
T10436 22-29 JJ denotes splenic
T10437 30-32 NNP denotes DC
T10438 33-40 VBZ denotes subsets
T10439 41-44 DT denotes The
T10440 45-54 NN denotes phenotype
T10441 55-57 IN denotes of
T10442 58-62 NNP denotes BMDC
T10443 63-66 VBD denotes was
T10444 67-77 VBN denotes determined
T10445 78-84 IN denotes before
T10446 85-88 CC denotes and
T10447 89-94 IN denotes after
T10448 95-97 CD denotes 24
T10449 98-102 NN denotes hour
T10450 103-110 NN denotes culture
T10451 111-113 IN denotes in
T10452 114-117 DT denotes the
T10453 118-126 NN denotes presence
T10454 127-129 IN denotes of
T10455 130-133 CD denotes 100
T10456 134-139 NNP denotes ng/mL
T10457 140-143 NNP denotes LPS
T10458 143-144 . denotes .
T10459 145-148 DT denotes The
T10460 149-154 NNS denotes cells
T10461 155-159 VBD denotes were
T10462 160-169 VBN denotes incubated
T10463 170-172 IN denotes in
T10464 173-176 NNP denotes PBS
T10465 177-181 IN denotes with
T10466 182-185 CD denotes 0.1
T10467 185-186 NN denotes %
T10468 187-191 JJ denotes NaN3
T10469 191-192 , denotes ,
T10470 193-194 CD denotes 1
T10471 194-195 NN denotes %
T10472 196-199 NNP denotes BSA
T10473 200-203 CC denotes and
T10474 204-206 CD denotes 10
T10475 206-207 NN denotes %
T10476 208-214 JJ denotes normal
T10477 215-221 NN denotes rabbit
T10478 222-227 NN denotes serum
T10479 228-231 IN denotes for
T10480 232-234 CD denotes 20
T10481 235-242 NNS denotes minutes
T10482 243-245 IN denotes on
T10483 246-249 NN denotes ice
T10484 249-250 , denotes ,
T10485 251-257 VBD denotes washed
T10486 258-261 CC denotes and
T10487 262-271 VBD denotes incubated
T10488 272-275 IN denotes for
T10489 276-278 CD denotes 30
T10490 279-286 NNS denotes minutes
T10491 287-289 IN denotes on
T10492 290-293 NN denotes ice
T10493 294-298 IN denotes with
T10494 299-303 NNP denotes Alex
T10495 304-317 VBD denotes Fluor-labeled
T10496 318-328 JJ denotes anti-CD11c
T10497 329-330 -LRB- denotes (
T10498 330-338 NNP denotes MCD11C20
T10499 338-339 , denotes ,
T10500 340-346 NNP denotes CALTAG
T10501 346-347 -RRB- denotes )
T10502 348-350 IN denotes in
T10503 351-362 NN denotes combination
T10504 363-367 IN denotes with
T10505 368-370 NNP denotes PE
T10506 370-371 : denotes -
T10507 372-381 NN denotes anti-CD40
T10508 382-383 -LRB- denotes (
T10509 383-387 CD denotes 3/23
T10510 387-388 , denotes ,
T10511 389-391 NNP denotes BD
T10512 392-403 NNP denotes Biosciences
T10513 403-404 -RRB- denotes )
T10514 404-405 , denotes ,
T10515 406-418 NN denotes PE-anti-CD80
T10516 419-420 -LRB- denotes (
T10517 420-427 NNP denotes 16-10A1
T10518 427-428 , denotes ,
T10519 429-440 NNP denotes eBioscience
T10520 440-441 -RRB- denotes )
T10521 441-442 , denotes ,
T10522 443-455 NNP denotes PE-anti-CD86
T10523 456-457 -LRB- denotes (
T10524 457-460 NNP denotes P03
T10525 460-462 CD denotes .1
T10526 462-463 , denotes ,
T10527 464-475 NNP denotes eBioscience
T10528 475-476 -RRB- denotes )
T10529 476-477 , denotes ,
T10530 478-490 NNP denotes PE-anti-CD14
T10531 491-492 -LRB- denotes (
T10532 492-495 NNP denotes Sa2
T10533 495-497 CD denotes .8
T10534 497-498 , denotes ,
T10535 499-510 NNP denotes eBioscience
T10536 510-511 -RRB- denotes )
T10537 512-515 CC denotes and
T10538 516-528 VBD denotes biotinylated
T10539 529-538 JJ denotes anti-TLR4
T10540 539-540 -LRB- denotes (
T10541 540-549 NNP denotes BioLegend
T10542 549-550 -RRB- denotes )
T10543 550-551 . denotes .
T10544 552-555 DT denotes The
T10545 556-561 NNS denotes cells
T10546 562-571 VBN denotes incubated
T10547 572-576 IN denotes with
T10548 577-589 JJ denotes biotinylated
T10549 590-599 JJ denotes anti-TLR4
T10550 599-600 NN denotes /
T10551 600-604 NN denotes MD-2
T10552 605-609 VBD denotes were
T10553 610-616 VBN denotes washed
T10554 617-622 RB denotes twice
T10555 623-626 CC denotes and
T10556 627-636 VBN denotes incubated
T10557 637-640 IN denotes for
T10558 641-643 CD denotes 30
T10559 644-651 NNS denotes minutes
T10560 652-654 IN denotes on
T10561 655-658 NN denotes ice
T10562 659-663 IN denotes with
T10563 664-673 NNP denotes avidin-PE
T10564 673-674 . denotes .
T10565 675-677 TO denotes To
T10566 678-685 VB denotes isolate
T10567 686-693 JJ denotes splenic
T10568 694-696 NNP denotes DC
T10569 696-697 , denotes ,
T10570 698-705 NNS denotes spleens
T10571 706-710 VBD denotes were
T10572 711-722 JJ denotes collagenase
T10573 723-731 VBN denotes digested
T10574 732-735 CC denotes and
T10575 736-743 JJ denotes subject
T10576 744-746 TO denotes to
T10577 747-754 NNP denotes Percoll
T10578 755-763 NN denotes gradient
T10579 764-778 NN denotes centrifugation
T10580 778-779 . denotes .
T10581 780-783 DT denotes The
T10582 784-789 NNS denotes bands
T10583 790-792 IN denotes at
T10584 793-796 DT denotes the
T10585 797-799 CD denotes 35
T10586 800-802 CD denotes 55
T10587 802-803 NN denotes %
T10588 804-813 NN denotes interface
T10589 814-818 VBD denotes were
T10590 819-828 VBN denotes collected
T10591 829-831 TO denotes to
T10592 832-837 VB denotes stain
T10593 838-842 IN denotes with
T10594 843-856 NNP denotes PE-anti-CD11c
T10595 857-858 -LRB- denotes (
T10596 858-861 NNP denotes HL3
T10597 861-862 , denotes ,
T10598 863-872 NNP denotes BioLegend
T10599 872-873 -RRB- denotes )
T10600 873-874 , denotes ,
T10601 875-890 NNP denotes APC-anti-PDCA-1
T10602 891-892 -LRB- denotes (
T10603 892-895 CD denotes 927
T10604 895-896 , denotes ,
T10605 897-906 NNP denotes BioLegend
T10606 906-907 -RRB- denotes )
T10607 908-911 CC denotes and
T10608 912-926 NNP denotes FITC-anti-CD8α
T10609 927-928 -LRB- denotes (
T10610 928-930 CD denotes 53
T10611 931-934 CD denotes 6.7
T10612 934-935 , denotes ,
T10613 936-938 NNP denotes BD
T10614 939-949 NNP denotes BioScience
T10615 949-950 -RRB- denotes )
T10616 950-951 . denotes .
T10617 952-955 DT denotes The
T10618 956-961 NNS denotes cells
T10619 962-966 VBD denotes were
T10620 967-973 VBN denotes washed
T10621 974-979 RB denotes twice
T10622 979-980 , denotes ,
T10623 981-986 VBN denotes fixed
T10624 987-989 IN denotes in
T10625 990-991 CD denotes 2
T10626 991-992 NN denotes %
T10627 993-1009 NN denotes paraformaldehyde
T10628 1009-1010 , denotes ,
T10629 1011-1014 CC denotes and
T10630 1015-1021 VBD denotes stored
T10631 1022-1024 IN denotes at
T10632 1025-1026 CD denotes 4
T10633 1026-1027 CD denotes °
T10634 1027-1028 NNP denotes C
T10635 1029-1034 IN denotes until
T10636 1035-1043 NN denotes analysis
T10637 1043-1044 . denotes .
T10638 1045-1049 NN denotes Flow
T10639 1050-1059 NN denotes cytometry
T10640 1060-1063 VBD denotes was
T10641 1064-1073 VBN denotes performed
T10642 1074-1076 IN denotes in
T10643 1077-1079 DT denotes an
T10644 1080-1085 NNP denotes Excel
T10645 1086-1087 -LRB- denotes (
T10646 1087-1094 NNP denotes Coulter
T10647 1094-1095 -RRB- denotes )
T10648 1096-1106 NN denotes instrument
T10649 1106-1107 . denotes .
T10650 1108-1112 NNP denotes Dead
T10651 1113-1118 NNS denotes cells
T10652 1119-1123 VBD denotes were
T10653 1124-1131 VBN denotes omitted
T10654 1132-1136 IN denotes from
T10655 1137-1140 DT denotes the
T10656 1141-1149 NN denotes analysis
T10657 1150-1152 IN denotes by
T10658 1153-1159 VBG denotes gating
T10659 1160-1162 IN denotes on
T10660 1163-1170 RB denotes forward
T10661 1171-1174 CC denotes and
T10662 1175-1177 CD denotes 90
T10663 1177-1178 NN denotes °
T10664 1179-1184 JJ denotes light
T10665 1185-1192 NN denotes scatter
T10666 1192-1193 , denotes ,
T10667 1194-1197 CC denotes and
T10668 1198-1204 CD denotes 10,000
T10669 1205-1210 NNS denotes cells
T10670 1211-1215 VBD denotes were
T10671 1216-1224 VBN denotes analyzed
T10672 1225-1227 IN denotes by
T10673 1228-1234 NNP denotes FlowJo
T10674 1235-1243 NN denotes software
T10675 1243-1244 . denotes .
R8584 T10432 T10438 nsubj Phenotype,subsets
R8585 T10433 T10432 prep of,Phenotype
R8586 T10434 T10433 pobj BMDC,of
R8587 T10435 T10432 cc and,Phenotype
R8588 T10436 T10437 compound splenic,DC
R8589 T10437 T10432 conj DC,Phenotype
R8590 T10438 T10438 ROOT subsets,subsets
R8591 T10439 T10440 det The,phenotype
R8592 T10440 T10444 nsubjpass phenotype,determined
R8593 T10441 T10440 prep of,phenotype
R8594 T10442 T10441 pobj BMDC,of
R8595 T10443 T10444 auxpass was,determined
R8596 T10444 T10438 ccomp determined,subsets
R8597 T10445 T10444 advmod before,determined
R8598 T10446 T10445 cc and,before
R8599 T10447 T10445 conj after,before
R8600 T10448 T10450 nummod 24,culture
R8601 T10449 T10450 compound hour,culture
R8602 T10450 T10447 pobj culture,after
R8603 T10451 T10450 prep in,culture
R8604 T10452 T10453 det the,presence
R8605 T10453 T10451 pobj presence,in
R8606 T10454 T10453 prep of,presence
R8607 T10455 T10457 nummod 100,LPS
R8608 T10456 T10457 compound ng/mL,LPS
R8609 T10457 T10454 pobj LPS,of
R8610 T10458 T10438 punct .,subsets
R8611 T10459 T10460 det The,cells
R8612 T10460 T10462 nsubjpass cells,incubated
R8613 T10461 T10462 auxpass were,incubated
R8614 T10462 T10462 ROOT incubated,incubated
R8615 T10463 T10462 prep in,incubated
R8616 T10464 T10463 pobj PBS,in
R8617 T10465 T10462 prep with,incubated
R8618 T10466 T10467 nummod 0.1,%
R8619 T10467 T10485 nsubj %,washed
R8620 T10468 T10467 amod NaN3,%
R8621 T10469 T10468 punct ",",NaN3
R8622 T10470 T10471 nummod 1,%
R8623 T10471 T10472 compound %,BSA
R8624 T10472 T10485 nsubj BSA,washed
R8625 T10473 T10472 cc and,BSA
R8626 T10474 T10475 nummod 10,%
R8627 T10475 T10478 nmod %,serum
R8628 T10476 T10478 amod normal,serum
R8629 T10477 T10478 compound rabbit,serum
R8630 T10478 T10472 conj serum,BSA
R8631 T10479 T10478 prep for,serum
R8632 T10480 T10481 nummod 20,minutes
R8633 T10481 T10479 pobj minutes,for
R8634 T10482 T10481 prep on,minutes
R8635 T10483 T10482 pobj ice,on
R8636 T10484 T10478 punct ",",serum
R8637 T10485 T10462 conj washed,incubated
R8638 T10486 T10485 cc and,washed
R8639 T10487 T10485 conj incubated,washed
R8640 T10488 T10487 prep for,incubated
R8641 T10489 T10490 nummod 30,minutes
R8642 T10490 T10488 pobj minutes,for
R8643 T10491 T10490 prep on,minutes
R8644 T10492 T10491 pobj ice,on
R8645 T10493 T10490 prep with,minutes
R8646 T10494 T10496 compound Alex,anti-CD11c
R8647 T10495 T10496 compound Fluor-labeled,anti-CD11c
R8648 T10496 T10493 pobj anti-CD11c,with
R8698 T10546 T10545 acl incubated,cells
R8699 T10547 T10546 prep with,incubated
R8700 T10548 T10551 amod biotinylated,MD-2
R8701 T10549 T10551 amod anti-TLR4,MD-2
R8702 T10550 T10551 compound /,MD-2
R8703 T10551 T10547 pobj MD-2,with
R8704 T10552 T10553 auxpass were,washed
R8705 T10553 T10553 ROOT washed,washed
R8706 T10554 T10553 advmod twice,washed
R8707 T10555 T10553 cc and,washed
R8708 T10556 T10553 conj incubated,washed
R8709 T10557 T10556 prep for,incubated
R8710 T10558 T10559 nummod 30,minutes
R8711 T10559 T10557 pobj minutes,for
R8712 T10560 T10559 prep on,minutes
R8713 T10561 T10560 pobj ice,on
R8714 T10562 T10559 prep with,minutes
R8715 T10563 T10562 pobj avidin-PE,with
R8716 T10564 T10553 punct .,washed
R8717 T10565 T10566 aux To,isolate
R8718 T10566 T10573 advcl isolate,digested
R8719 T10567 T10568 amod splenic,DC
R8720 T10568 T10566 dobj DC,isolate
R8721 T10569 T10573 punct ",",digested
R8722 T10570 T10573 nsubjpass spleens,digested
R8723 T10571 T10573 auxpass were,digested
R8724 T10572 T10573 advmod collagenase,digested
R8725 T10573 T10573 ROOT digested,digested
R8726 T10574 T10573 cc and,digested
R8727 T10575 T10573 conj subject,digested
R8728 T10576 T10575 prep to,subject
R8729 T10577 T10579 compound Percoll,centrifugation
R8730 T10578 T10579 compound gradient,centrifugation
R8731 T10579 T10576 pobj centrifugation,to
R8732 T10580 T10573 punct .,digested
R8733 T10581 T10582 det The,bands
R8734 T10582 T10590 nsubjpass bands,collected
R8735 T10583 T10582 prep at,bands
R8736 T10584 T10585 det the,35
R8737 T10585 T10583 pobj 35,at
R8738 T10586 T10587 nummod 55,%
R8739 T10587 T10588 compound %,interface
R8740 T10588 T10590 nsubjpass interface,collected
R8741 T10589 T10590 auxpass were,collected
R8742 T10590 T10590 ROOT collected,collected
R8743 T10591 T10592 aux to,stain
R8744 T10592 T10590 xcomp stain,collected
R8745 T10593 T10592 prep with,stain
R8746 T10594 T10593 pobj PE-anti-CD11c,with
R8747 T10595 T10594 punct (,PE-anti-CD11c
R8748 T10596 T10594 appos HL3,PE-anti-CD11c
R8749 T10597 T10596 punct ",",HL3
R8750 T10598 T10596 conj BioLegend,HL3
R8751 T10599 T10594 punct ),PE-anti-CD11c
R8752 T10600 T10594 punct ",",PE-anti-CD11c
R8753 T10601 T10594 conj APC-anti-PDCA-1,PE-anti-CD11c
R8754 T10602 T10601 punct (,APC-anti-PDCA-1
R8755 T10603 T10601 appos 927,APC-anti-PDCA-1
R8756 T10604 T10601 punct ",",APC-anti-PDCA-1
R8757 T10605 T10601 conj BioLegend,APC-anti-PDCA-1
R8758 T10606 T10605 punct ),BioLegend
R8759 T10607 T10605 cc and,BioLegend
R8760 T10608 T10605 conj FITC-anti-CD8α,BioLegend
R8761 T10609 T10608 punct (,FITC-anti-CD8α
R8762 T10610 T10608 appos 53,FITC-anti-CD8α
R8763 T10611 T10608 appos 6.7,FITC-anti-CD8α
R8764 T10612 T10608 punct ",",FITC-anti-CD8α
R8765 T10613 T10614 compound BD,BioScience
R8766 T10614 T10608 appos BioScience,FITC-anti-CD8α
R8767 T10615 T10608 punct ),FITC-anti-CD8α
R8768 T10616 T10590 punct .,collected
R8769 T10617 T10618 det The,cells
R8770 T10618 T10620 nsubjpass cells,washed
R8771 T10619 T10620 auxpass were,washed
R8772 T10620 T10620 ROOT washed,washed
R8773 T10621 T10620 advmod twice,washed
R8774 T10622 T10620 punct ",",washed
R8775 T10623 T10620 conj fixed,washed
R8776 T10624 T10623 prep in,fixed
R8777 T10625 T10626 nummod 2,%
R8778 T10626 T10627 compound %,paraformaldehyde
R8779 T10627 T10624 pobj paraformaldehyde,in
R8780 T10628 T10623 punct ",",fixed
R8781 T10629 T10623 cc and,fixed
R8782 T10630 T10623 conj stored,fixed
R8783 T10631 T10630 prep at,stored
R8784 T10632 T10633 nummod 4,°
R8785 T10633 T10631 pobj °,at
R8786 T10634 T10630 npadvmod C,stored
R8787 T10635 T10623 conj until,fixed
R8788 T10636 T10635 pobj analysis,until
R8789 T10637 T10620 punct .,washed
R8790 T10638 T10639 compound Flow,cytometry
R8791 T10639 T10641 nsubjpass cytometry,performed
R8792 T10640 T10641 auxpass was,performed
R8793 T10641 T10641 ROOT performed,performed
R8794 T10642 T10641 prep in,performed
R8795 T10643 T10648 det an,instrument
R8796 T10644 T10648 nmod Excel,instrument
R8797 T10645 T10646 punct (,Coulter
R8798 T10646 T10644 appos Coulter,Excel
R8799 T10647 T10644 punct ),Excel
R8800 T10648 T10642 pobj instrument,in
R8801 T10649 T10641 punct .,performed
R8802 T10650 T10651 compound Dead,cells
R8803 T10651 T10653 nsubjpass cells,omitted
R8804 T10652 T10653 auxpass were,omitted
R8805 T10653 T10653 ROOT omitted,omitted
R8806 T10654 T10653 prep from,omitted
R8807 T10655 T10656 det the,analysis
R8808 T10656 T10654 pobj analysis,from
R8809 T10657 T10653 prep by,omitted
R8810 T10658 T10657 pcomp gating,by
R8811 T10659 T10658 prep on,gating
R8812 T10660 T10659 pobj forward,on
R8813 T10661 T10660 cc and,forward
R8814 T10662 T10663 nummod 90,°
R8815 T10663 T10665 npadvmod °,scatter
R8816 T10664 T10665 amod light,scatter
R8817 T10665 T10660 conj scatter,forward
R8818 T10666 T10665 punct ",",scatter
R8819 T10667 T10665 cc and,scatter
R8820 T10668 T10669 nummod "10,000",cells
R8821 T10669 T10671 nsubjpass cells,analyzed
R8822 T10670 T10671 auxpass were,analyzed
R8823 T10671 T10653 conj analyzed,omitted
R8824 T10672 T10671 agent by,analyzed
R8825 T10673 T10674 compound FlowJo,software
R8826 T10674 T10672 pobj software,by
R8827 T10675 T10671 punct .,analyzed
R8649 T10497 T10498 punct (,MCD11C20
R8650 T10498 T10496 appos MCD11C20,anti-CD11c
R8651 T10499 T10500 punct ",",CALTAG
R8652 T10500 T10498 appos CALTAG,MCD11C20
R8653 T10501 T10498 punct ),MCD11C20
R8654 T10502 T10498 prep in,MCD11C20
R8655 T10503 T10502 pobj combination,in
R8656 T10504 T10503 prep with,combination
R8657 T10505 T10507 compound PE,anti-CD40
R8658 T10506 T10507 punct -,anti-CD40
R8659 T10507 T10504 pobj anti-CD40,with
R8660 T10508 T10507 punct (,anti-CD40
R8661 T10509 T10507 appos 3/23,anti-CD40
R8662 T10510 T10507 punct ",",anti-CD40
R8663 T10511 T10512 compound BD,Biosciences
R8664 T10512 T10507 appos Biosciences,anti-CD40
R8665 T10513 T10507 punct ),anti-CD40
R8666 T10514 T10507 punct ",",anti-CD40
R8667 T10515 T10507 appos PE-anti-CD80,anti-CD40
R8668 T10516 T10517 punct (,16-10A1
R8669 T10517 T10515 appos 16-10A1,PE-anti-CD80
R8670 T10518 T10517 punct ",",16-10A1
R8671 T10519 T10517 appos eBioscience,16-10A1
R8672 T10520 T10517 punct ),16-10A1
R8673 T10521 T10517 punct ",",16-10A1
R8674 T10522 T10517 conj PE-anti-CD86,16-10A1
R8675 T10523 T10522 punct (,PE-anti-CD86
R8676 T10524 T10525 compound P03,.1
R8677 T10525 T10522 appos .1,PE-anti-CD86
R8678 T10526 T10522 punct ",",PE-anti-CD86
R8679 T10527 T10522 conj eBioscience,PE-anti-CD86
R8680 T10528 T10522 punct ),PE-anti-CD86
R8681 T10529 T10522 punct ",",PE-anti-CD86
R8682 T10530 T10522 conj PE-anti-CD14,PE-anti-CD86
R8683 T10531 T10530 punct (,PE-anti-CD14
R8684 T10532 T10533 compound Sa2,.8
R8685 T10533 T10530 appos .8,PE-anti-CD14
R8686 T10534 T10530 punct ",",PE-anti-CD14
R8687 T10535 T10530 conj eBioscience,PE-anti-CD14
R8688 T10536 T10535 punct ),eBioscience
R8689 T10537 T10515 cc and,PE-anti-CD80
R8690 T10538 T10539 compound biotinylated,anti-TLR4
R8691 T10539 T10515 conj anti-TLR4,PE-anti-CD80
R8692 T10540 T10541 punct (,BioLegend
R8693 T10541 T10539 appos BioLegend,anti-TLR4
R8694 T10542 T10541 punct ),BioLegend
R8695 T10543 T10485 punct .,washed
R8696 T10544 T10545 det The,cells
R8697 T10545 T10553 nsubjpass cells,washed

UBERON-AE

Id Subject Object Predicate Lexical cue
T10421 222-227 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T10422 698-705 http://purl.obolibrary.org/obo/UBERON_0002106 denotes spleens

GO-CC

Id Subject Object Predicate Lexical cue
T10919 149-154 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10920 556-561 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10921 956-961 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10922 1113-1118 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10923 1205-1210 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10924 875-878 http://purl.obolibrary.org/obo/GO_0005680 denotes APC

sentences

Id Subject Object Predicate Lexical cue
T10423 0-40 Sentence denotes Phenotype of BMDC and splenic DC subsets
T10424 41-144 Sentence denotes The phenotype of BMDC was determined before and after 24 hour culture in the presence of 100 ng/mL LPS.
T10425 145-551 Sentence denotes The cells were incubated in PBS with 0.1% NaN3, 1% BSA and 10% normal rabbit serum for 20 minutes on ice, washed and incubated for 30 minutes on ice with Alex Fluor-labeled anti-CD11c (MCD11C20, CALTAG) in combination with PE- anti-CD40 (3/23, BD Biosciences), PE-anti-CD80 (16-10A1, eBioscience), PE-anti-CD86 (P03.1, eBioscience), PE-anti-CD14 (Sa2.8, eBioscience) and biotinylated anti-TLR4 (BioLegend).
T10426 552-674 Sentence denotes The cells incubated with biotinylated anti-TLR4/MD-2 were washed twice and incubated for 30 minutes on ice with avidin-PE.
T10427 675-779 Sentence denotes To isolate splenic DC, spleens were collagenase digested and subject to Percoll gradient centrifugation.
T10428 780-951 Sentence denotes The bands at the 35–55% interface were collected to stain with PE-anti-CD11c (HL3, BioLegend), APC-anti-PDCA-1 (927, BioLegend) and FITC-anti-CD8α (53–6.7, BD BioScience).
T10429 952-1044 Sentence denotes The cells were washed twice, fixed in 2% paraformaldehyde, and stored at 4°C until analysis.
T10430 1045-1107 Sentence denotes Flow cytometry was performed in an Excel (Coulter) instrument.
T10431 1108-1244 Sentence denotes Dead cells were omitted from the analysis by gating on forward and 90° light scatter, and 10,000 cells were analyzed by FlowJo software.
T195 0-40 Sentence denotes Phenotype of BMDC and splenic DC subsets
T196 41-144 Sentence denotes The phenotype of BMDC was determined before and after 24 hour culture in the presence of 100 ng/mL LPS.
T197 145-551 Sentence denotes The cells were incubated in PBS with 0.1% NaN3, 1% BSA and 10% normal rabbit serum for 20 minutes on ice, washed and incubated for 30 minutes on ice with Alex Fluor-labeled anti-CD11c (MCD11C20, CALTAG) in combination with PE- anti-CD40 (3/23, BD Biosciences), PE-anti-CD80 (16-10A1, eBioscience), PE-anti-CD86 (P03.1, eBioscience), PE-anti-CD14 (Sa2.8, eBioscience) and biotinylated anti-TLR4 (BioLegend).
T198 552-674 Sentence denotes The cells incubated with biotinylated anti-TLR4/MD-2 were washed twice and incubated for 30 minutes on ice with avidin-PE.
T199 675-779 Sentence denotes To isolate splenic DC, spleens were collagenase digested and subject to Percoll gradient centrifugation.
T200 780-951 Sentence denotes The bands at the 35–55% interface were collected to stain with PE-anti-CD11c (HL3, BioLegend), APC-anti-PDCA-1 (927, BioLegend) and FITC-anti-CD8α (53–6.7, BD BioScience).
T201 952-1044 Sentence denotes The cells were washed twice, fixed in 2% paraformaldehyde, and stored at 4°C until analysis.
T202 1045-1107 Sentence denotes Flow cytometry was performed in an Excel (Coulter) instrument.
T203 1108-1244 Sentence denotes Dead cells were omitted from the analysis by gating on forward and 90° light scatter, and 10,000 cells were analyzed by FlowJo software.

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T10925 323-328 Protein denotes CD11c
T10926 330-338 Protein denotes MCD11C20
T10927 478-485 Protein denotes PE-anti
T10928 486-490 Protein denotes CD14
T10929 664-673 Protein denotes avidin-PE
T10930 711-722 Protein denotes collagenase
T10931 851-856 Protein denotes CD11c
T10932 858-861 Protein denotes HL3
T10933 912-921 Protein denotes FITC-anti
T10934 922-926 Protein denotes CD8α

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T10902 323-328 P20702 denotes CD11c
T10903 377-381 P25942 denotes CD40
T10904 414-418 P33681 denotes CD80
T10905 451-455 P42081 denotes CD86
T10906 486-490 P08571 denotes CD14
T10907 534-538 O00206 denotes TLR4
T10908 595-599 O00206 denotes TLR4
T10909 851-856 P20702 denotes CD11c