PMC:3279418 / 27835-27945 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T10248	0-5	IN	denotes	After
T10249	6-13	DT	denotes	another
T10250	14-16	CD	denotes	24
T10251	17-22	NN	denotes	hours
T10252	22-23	-COMMA-	denotes	,
T10253	24-29	NN	denotes	cells
T10254	30-34	VB	denotes	were
T10255	35-40	VB	denotes	lysed
T10256	41-43	TO	denotes	to
T10257	44-51	VB	denotes	confirm
T10258	52-55	DT	denotes	the
T10259	56-68	NN	denotes	FLAG-SHARPIN
T10260	69-79	NN	denotes	expression
T10261	80-82	IN	denotes	by
T10262	83-94	NN	denotes	immunoblots
T10263	95-99	IN	denotes	with
T10264	100-109	NN	denotes	anti-FLAG
R8463	T10251	T10248	arg2Of	hours,After
R8464	T10251	T10249	arg1Of	hours,another
R8465	T10251	T10250	arg1Of	hours,24
R8466	T10253	T10254	arg1Of	cells,were
R8467	T10253	T10255	arg2Of	cells,lysed
R8468	T10253	T10257	arg1Of	cells,confirm
R8469	T10255	T10248	arg1Of	lysed,After
R8470	T10255	T10252	arg1Of	lysed,","
R8471	T10255	T10254	arg2Of	lysed,were
R8472	T10257	T10255	arg3Of	confirm,lysed
R8473	T10257	T10256	arg1Of	confirm,to
R8474	T10260	T10257	arg2Of	expression,confirm
R8475	T10260	T10258	arg1Of	expression,the
R8476	T10260	T10259	arg1Of	expression,FLAG-SHARPIN
R8477	T10260	T10261	arg1Of	expression,by
R8478	T10262	T10261	arg2Of	immunoblots,by
R8479	T10262	T10263	arg1Of	immunoblots,with
R8480	T10264	T10263	arg2Of	anti-FLAG,with

Id	Subject	Object	Predicate	Lexical cue
T10097	0-5	IN	denotes	After
T10098	6-13	DT	denotes	another
T10099	14-16	CD	denotes	24
T10100	17-22	NNS	denotes	hours
T10101	22-23	,	denotes	,
T10102	24-29	NNS	denotes	cells
T10103	30-34	VBD	denotes	were
T10104	35-40	VBN	denotes	lysed
T10105	41-43	TO	denotes	to
T10106	44-51	VB	denotes	confirm
T10107	52-55	DT	denotes	the
T10108	56-68	NNP	denotes	FLAG-SHARPIN
T10109	69-79	NN	denotes	expression
T10110	80-82	IN	denotes	by
T10111	83-94	NNS	denotes	immunoblots
T10112	95-99	IN	denotes	with
T10113	100-109	JJ	denotes	anti-FLAG
T10114	109-110	.	denotes	.
R8312	T10097	T10104	prep	After,lysed
R8313	T10098	T10100	det	another,hours
R8314	T10099	T10100	nummod	24,hours
R8315	T10100	T10097	pobj	hours,After
R8316	T10101	T10104	punct	",",lysed
R8317	T10102	T10104	nsubjpass	cells,lysed
R8318	T10103	T10104	auxpass	were,lysed
R8319	T10104	T10104	ROOT	lysed,lysed
R8320	T10105	T10106	aux	to,confirm
R8321	T10106	T10104	advcl	confirm,lysed
R8322	T10107	T10109	det	the,expression
R8323	T10108	T10109	compound	FLAG-SHARPIN,expression
R8324	T10109	T10106	dobj	expression,confirm
R8325	T10110	T10109	prep	by,expression
R8326	T10111	T10110	pobj	immunoblots,by
R8327	T10112	T10111	prep	with,immunoblots
R8328	T10113	T10112	pobj	anti-FLAG,with
R8329	T10114	T10104	punct	.,lysed

Id	Subject	Object	Predicate	Lexical cue
T10301	24-29	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells

Id	Subject	Object	Predicate	Lexical cue
T9967	0-110	Sentence	denotes	After another 24 hours, cells were lysed to confirm the FLAG-SHARPIN expression by immunoblots with anti-FLAG.
T189	0-110	Sentence	denotes	After another 24 hours, cells were lysed to confirm the FLAG-SHARPIN expression by immunoblots with anti-FLAG.

Id	Subject	Object	Predicate	Lexical cue	Speculation
T10321	61-68	Protein	denotes	SHARPIN
T10322	69-79	Gene_expression	denotes	expression	true
R8506	T10321	T10322	themeOf	SHARPIN,expression

Id	Subject	Object	Predicate	Lexical cue
T10331	56-60	Protein	denotes	FLAG
T10332	61-68	Protein	denotes	SHARPIN
T10333	69-79	Gene_expression	denotes	expression
T10334	69-79	Gene_expression	denotes	expression
R8508	T10331	T10333	themeOf	FLAG,expression
R8509	T10332	T10334	themeOf	SHARPIN,expression

Id	Subject	Object	Predicate	Lexical cue	Speculation
T9958	61-68	Protein	denotes	SHARPIN
T9959	69-79	Gene_expression	denotes	expression	true
R8183	T9958	T9959	themeOf	SHARPIN,expression

Id	Subject	Object	Predicate	Lexical cue
T10289	61-68	Q9H0F6	denotes	SHARPIN

Id	Subject	Object	Predicate	Lexical cue
T9951	61-68	Protein	denotes	SHARPIN
T9952	69-79	Gene_expression	denotes	expression
R8181	T9951	T9952	themeOf	SHARPIN,expression