PMC:3279418 / 27664-27770 JSON TXT

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Id	Subject	Object	Predicate	Lexical cue
T10222	0-5	IN	denotes	After
T10223	6-15	JJ	denotes	overnight
T10224	16-26	NN	denotes	incubation
T10225	26-27	-COMMA-	denotes	,
T10226	28-31	CD	denotes	200
T10227	32-34	NN	denotes	ng
T10228	35-48	NN	denotes	pFLAG-SHARPIN
T10229	49-57	NN	denotes	plasmids
T10230	58-62	VB	denotes	were
T10231	63-74	VB	denotes	transfected
T10232	75-79	IN	denotes	with
T10233	80-83	CD	denotes	0.5
T10234	84-86	NN	denotes	ul
T10235	87-100	NN	denotes	Lipofectamine
T10236	101-105	CD	denotes	2000
R8437	T10224	T10222	arg2Of	incubation,After
R8438	T10224	T10223	arg1Of	incubation,overnight
R8439	T10229	T10226	arg1Of	plasmids,200
R8440	T10229	T10227	arg1Of	plasmids,ng
R8441	T10229	T10228	arg1Of	plasmids,pFLAG-SHARPIN
R8442	T10229	T10230	arg1Of	plasmids,were
R8443	T10229	T10231	arg2Of	plasmids,transfected
R8444	T10231	T10222	arg1Of	transfected,After
R8445	T10231	T10225	arg1Of	transfected,","
R8446	T10231	T10230	arg2Of	transfected,were
R8447	T10231	T10232	arg1Of	transfected,with
R8448	T10235	T10232	arg2Of	Lipofectamine,with
R8449	T10235	T10233	arg1Of	Lipofectamine,0.5
R8450	T10235	T10234	arg1Of	Lipofectamine,ul
R8451	T10235	T10236	arg1Of	Lipofectamine,2000

Id	Subject	Object	Predicate	Lexical cue
T10069	0-5	IN	denotes	After
T10070	6-15	JJ	denotes	overnight
T10071	16-26	NN	denotes	incubation
T10072	26-27	,	denotes	,
T10073	28-31	CD	denotes	200
T10074	32-34	NN	denotes	ng
T10075	35-48	NN	denotes	pFLAG-SHARPIN
T10076	49-57	NNS	denotes	plasmids
T10077	58-62	VBD	denotes	were
T10078	63-74	VBN	denotes	transfected
T10079	75-79	IN	denotes	with
T10080	80-83	CD	denotes	0.5
T10081	84-86	NN	denotes	ul
T10082	87-100	NNP	denotes	Lipofectamine
T10083	101-105	CD	denotes	2000
T10084	105-106	.	denotes	.
R8284	T10069	T10078	prep	After,transfected
R8285	T10070	T10071	amod	overnight,incubation
R8286	T10071	T10069	pobj	incubation,After
R8287	T10072	T10078	punct	",",transfected
R8288	T10073	T10076	nummod	200,plasmids
R8289	T10074	T10076	compound	ng,plasmids
R8290	T10075	T10076	compound	pFLAG-SHARPIN,plasmids
R8291	T10076	T10078	nsubjpass	plasmids,transfected
R8292	T10077	T10078	auxpass	were,transfected
R8293	T10078	T10078	ROOT	transfected,transfected
R8294	T10079	T10078	prep	with,transfected
R8295	T10080	T10081	nummod	0.5,ul
R8296	T10081	T10082	compound	ul,Lipofectamine
R8297	T10082	T10079	pobj	Lipofectamine,with
R8298	T10083	T10082	nummod	2000,Lipofectamine
R8299	T10084	T10078	punct	.,transfected

Id	Subject	Object	Predicate	Lexical cue
T9965	0-106	Sentence	denotes	After overnight incubation, 200 ng pFLAG-SHARPIN plasmids were transfected with 0.5 ul Lipofectamine 2000.
T187	0-106	Sentence	denotes	After overnight incubation, 200 ng pFLAG-SHARPIN plasmids were transfected with 0.5 ul Lipofectamine 2000.

Id	Subject	Object	Predicate	Lexical cue
T10320	41-48	Protein	denotes	SHARPIN

Id	Subject	Object	Predicate	Lexical cue
T9957	41-48	Protein	denotes	SHARPIN

Id	Subject	Object	Predicate	Lexical cue
T10288	41-48	Q9H0F6	denotes	SHARPIN

Id	Subject	Object	Predicate	Lexical cue
T9950	41-48	Protein	denotes	SHARPIN