> top > docs > PMC:3279418 > annotations

PMC:3279418 JSONTXT

Annnotations TAB JSON ListView MergeView

2_test

Id Subject Object Predicate Lexical cue
22348129-11178875-89575657 1877-1878 11178875 denotes 1
22348129-17538631-89575658 1943-1944 17538631 denotes 2
22348129-17538631-89575659 2250-2251 17538631 denotes 2
22348129-17538631-89575660 2442-2443 17538631 denotes 2
22348129-10201907-89575661 2446-2447 10201907 denotes 4
22348129-11241277-89575662 2793-2794 11241277 denotes 5
22348129-11241277-89575663 2895-2896 11241277 denotes 5
22348129-11241277-89575664 3084-3085 11241277 denotes 5
22348129-19650867-89575665 3291-3292 19650867 denotes 6
22348129-21455173-89575666 3485-3486 21455173 denotes 7
22348129-21455180-89575667 3489-3490 21455180 denotes 9
22348129-21455173-89575668 3660-3661 21455173 denotes 7
22348129-21455180-89575669 3664-3665 21455180 denotes 9
22348129-20853490-89575670 3816-3818 20853490 denotes 10
22348129-2031185-89575671 4898-4900 2031185 denotes 11
22348129-17545200-89575672 4917-4919 17545200 denotes 12
22348129-8362989-89575673 5505-5506 8362989 denotes 3
22348129-20193011-89575674 7376-7378 20193011 denotes 13
22348129-20193011-89575675 7566-7568 20193011 denotes 13
22348129-15593126-89575676 7612-7614 15593126 denotes 14
22348129-17589544-89575677 9687-9689 17589544 denotes 15
22348129-17170756-89575678 9693-9695 17170756 denotes 16
22348129-16691244-89575679 9999-10001 16691244 denotes 17
22348129-19686699-89575680 10005-10007 19686699 denotes 18
22348129-17538631-89575681 10941-10942 17538631 denotes 2
22348129-17538631-89575682 12058-12059 17538631 denotes 2
22348129-19252480-89575683 13448-13450 19252480 denotes 19
22348129-9174615-89575684 13704-13706 9174615 denotes 20
22348129-11254683-89575685 13710-13712 11254683 denotes 21
22348129-15334086-89575686 14007-14009 15334086 denotes 22
22348129-11861597-89575687 15175-15177 11861597 denotes 23
22348129-12692549-89575688 15180-15182 12692549 denotes 27
22348129-11241277-89575689 16831-16832 11241277 denotes 5
22348129-11034386-89575690 17385-17387 11034386 denotes 28
22348129-14978071-89575691 17390-17392 14978071 denotes 30
22348129-21709223-89575692 17684-17686 21709223 denotes 31
22348129-19720113-89575693 20480-20482 19720113 denotes 32
22348129-21455173-89575694 20842-20843 21455173 denotes 7
22348129-21455181-89575695 20847-20848 21455181 denotes 8
22348129-7792947-89575696 21082-21084 7792947 denotes 33
22348129-21620685-89575697 21152-21154 21620685 denotes 34
22348129-19720113-89575698 21340-21342 19720113 denotes 32
22348129-17979840-89575699 21346-21348 17979840 denotes 35
22348129-16267105-89575700 21387-21389 16267105 denotes 36
22348129-16878026-89575701 21393-21395 16878026 denotes 37
22348129-11241277-89575702 21461-21462 11241277 denotes 5
22348129-11241277-89575703 21665-21666 11241277 denotes 5
22348129-8630732-89575704 21929-21931 8630732 denotes 38
22348129-8766560-89575705 21935-21937 8766560 denotes 39
22348129-8894351-89575706 21987-21989 8894351 denotes 40
22348129-7930584-89575707 21992-21994 7930584 denotes 42
22348129-20811394-89575708 22562-22564 20811394 denotes 43
22348129-19855404-89575709 22825-22827 19855404 denotes 44
22348129-11009421-89575710 23053-23055 11009421 denotes 45
22348129-18246070-89575711 23059-23061 18246070 denotes 46
22348129-12075355-89575712 23239-23241 12075355 denotes 47
22348129-17361131-89575713 23244-23246 17361131 denotes 50
22348129-11869686-89575714 23972-23974 11869686 denotes 51
22348129-17513725-89575715 23978-23980 17513725 denotes 52
22348129-21455173-89575716 24091-24092 21455173 denotes 7
22348129-21455180-89575717 24096-24097 21455180 denotes 9
22348129-15229469-89575718 24792-24794 15229469 denotes 53
22348129-19686699-89575719 24986-24988 19686699 denotes 18
22348129-21455173-89575720 25163-25164 21455173 denotes 7
22348129-17485153-89575721 28146-28148 17485153 denotes 54
22348129-10384102-89575722 28152-28154 10384102 denotes 55
22348129-11846609-89575723 31444-31446 11846609 denotes 56
T60732 1877-1878 11178875 denotes 1
T3640 1943-1944 17538631 denotes 2
T64062 2250-2251 17538631 denotes 2
T26028 2442-2443 17538631 denotes 2
T66489 2446-2447 10201907 denotes 4
T8236 2793-2794 11241277 denotes 5
T15760 2895-2896 11241277 denotes 5
T58148 3084-3085 11241277 denotes 5
T59353 3291-3292 19650867 denotes 6
T71923 3485-3486 21455173 denotes 7
T56508 3489-3490 21455180 denotes 9
T89522 3660-3661 21455173 denotes 7
T20207 3664-3665 21455180 denotes 9
T46751 3816-3818 20853490 denotes 10
T88551 4898-4900 2031185 denotes 11
T69510 4917-4919 17545200 denotes 12
T81551 5505-5506 8362989 denotes 3
T44705 7376-7378 20193011 denotes 13
T94367 7566-7568 20193011 denotes 13
T12018 7612-7614 15593126 denotes 14
T52721 9687-9689 17589544 denotes 15
T13133 9693-9695 17170756 denotes 16
T64617 9999-10001 16691244 denotes 17
T40972 10005-10007 19686699 denotes 18
T51169 10941-10942 17538631 denotes 2
T59339 12058-12059 17538631 denotes 2
T98878 13448-13450 19252480 denotes 19
T58035 13704-13706 9174615 denotes 20
T42368 13710-13712 11254683 denotes 21
T3457 14007-14009 15334086 denotes 22
T32235 15175-15177 11861597 denotes 23
T92737 15180-15182 12692549 denotes 27
T76846 16831-16832 11241277 denotes 5
T94596 17385-17387 11034386 denotes 28
T64234 17390-17392 14978071 denotes 30
T67158 17684-17686 21709223 denotes 31
T75014 20480-20482 19720113 denotes 32
T48730 20842-20843 21455173 denotes 7
T86456 20847-20848 21455181 denotes 8
T13816 21082-21084 7792947 denotes 33
T92727 21152-21154 21620685 denotes 34
T91786 21340-21342 19720113 denotes 32
T32556 21346-21348 17979840 denotes 35
T39324 21387-21389 16267105 denotes 36
T67123 21393-21395 16878026 denotes 37
T42257 21461-21462 11241277 denotes 5
T36921 21665-21666 11241277 denotes 5
T93315 21929-21931 8630732 denotes 38
T8547 21935-21937 8766560 denotes 39
T53614 21987-21989 8894351 denotes 40
T53448 21992-21994 7930584 denotes 42
T3770 22562-22564 20811394 denotes 43
T93400 22825-22827 19855404 denotes 44
T12295 23053-23055 11009421 denotes 45
T89481 23059-23061 18246070 denotes 46
T82809 23239-23241 12075355 denotes 47
T7028 23244-23246 17361131 denotes 50
T8190 23972-23974 11869686 denotes 51
T11191 23978-23980 17513725 denotes 52
T86732 24091-24092 21455173 denotes 7
T38809 24096-24097 21455180 denotes 9
T77167 24792-24794 15229469 denotes 53
T66479 24986-24988 19686699 denotes 18
T44614 25163-25164 21455173 denotes 7
T13443 28146-28148 17485153 denotes 54
T92008 28152-28154 10384102 denotes 55
T6565 31444-31446 11846609 denotes 56

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T399 0-7 NN denotes SHARPIN
T400 8-10 VB denotes Is
T401 11-20 JJ denotes Essential
T402 21-24 IN denotes for
T403 25-33 NN denotes Cytokine
T404 34-44 NN denotes Production
T405 44-45 -COMMA- denotes ,
T406 46-51 NN denotes NF-κB
T407 52-61 NN denotes Signaling
T408 61-62 -COMMA- denotes ,
T409 63-66 CC denotes and
T410 67-76 NN denotes Induction
T411 77-79 IN denotes of
T412 80-83 NN denotes Th1
T413 84-99 NN denotes Differentiation
T414 100-102 IN denotes by
T415 103-112 JJ denotes Dendritic
T416 113-118 NN denotes Cells
T417 165-176 JJ denotes Spontaneous
T418 177-186 NN denotes mutations
T419 187-189 IN denotes of
T420 190-193 DT denotes the
T421 194-201 NNP denotes Sharpin
T422 202-203 -LRB- denotes (
T423 203-219 JJ denotes SHANK-associated
T424 220-222 NN denotes RH
T425 223-241 JJ denotes domain-interacting
T426 242-249 NN denotes protein
T427 249-250 -COMMA- denotes ,
T428 251-256 JJ denotes other
T429 257-264 NN denotes aliases
T430 264-265 -COLON- denotes :
T431 266-272 NN denotes Rbckl1
T432 272-273 -COMMA- denotes ,
T433 274-279 NN denotes Sipl1
T434 279-280 -RRB- denotes )
T435 281-285 NN denotes gene
T436 286-288 IN denotes in
T437 289-293 NN denotes mice
T438 294-300 VB denotes result
T439 301-303 IN denotes in
T440 304-312 JJ denotes systemic
T441 313-325 NN denotes inflammation
T442 326-330 WDT denotes that
T443 331-333 VB denotes is
T444 334-347 VB denotes characterized
T445 348-350 IN denotes by
T446 351-358 JJ denotes chronic
T447 359-372 JJ denotes proliferative
T448 373-383 NN denotes dermatitis
T449 384-387 CC denotes and
T450 388-400 VB denotes dysregulated
T451 401-410 NN denotes secretion
T452 411-413 IN denotes of
T453 414-415 NN denotes T
T454 416-423 NN denotes helper1
T455 424-425 -LRB- denotes (
T456 425-428 NN denotes Th1
T457 428-429 -RRB- denotes )
T458 430-433 CC denotes and
T459 434-437 NN denotes Th2
T460 438-447 NN denotes cytokines
T461 449-452 DT denotes The
T462 453-461 JJ denotes cellular
T463 462-465 CC denotes and
T464 466-475 JJ denotes molecular
T465 476-486 NN denotes mechanisms
T466 487-497 VB denotes underlying
T467 498-502 DT denotes this
T468 503-515 JJ denotes inflammatory
T469 516-525 NN denotes phenotype
T470 526-532 VB denotes remain
T471 533-540 JJ denotes elusive
T472 542-551 JJ denotes Dendritic
T473 552-557 NN denotes cells
T474 558-561 MD denotes may
T475 562-572 VB denotes contribute
T476 573-575 TO denotes to
T477 576-579 DT denotes the
T478 580-590 NN denotes initiation
T479 591-594 CC denotes and
T480 595-606 NN denotes progression
T481 607-609 IN denotes of
T482 610-613 DT denotes the
T483 614-623 NN denotes phenotype
T484 624-626 IN denotes of
T485 627-644 JJ denotes SHARPIN-deficient
T486 645-649 NN denotes mice
T487 650-657 IN denotes because
T488 658-660 IN denotes of
T489 661-666 PRP-DOLLAR- denotes their
T490 667-674 JJ denotes pivotal
T491 675-679 NN denotes role
T492 680-682 IN denotes in
T493 683-689 JJ denotes innate
T494 690-693 CC denotes and
T495 694-702 JJ denotes adaptive
T496 703-711 NN denotes immunity
T497 713-717 RB denotes Here
T498 718-720 PRP denotes we
T499 721-725 VB denotes show
T500 726-728 IN denotes by
T501 729-733 NN denotes flow
T502 734-743 NN denotes cytometry
T503 744-748 IN denotes that
T504 749-757 NN denotes SHARPIN-
T505 758-768 NN denotes deficiency
T506 769-772 VB denotes did
T507 773-776 RB denotes not
T508 777-782 VB denotes alter
T509 783-786 DT denotes the
T510 787-799 NN denotes distribution
T511 800-802 IN denotes of
T512 803-812 JJ denotes different
T513 813-815 NN denotes DC
T514 816-824 NN denotes subtypes
T515 825-827 IN denotes in
T516 828-831 DT denotes the
T517 832-838 NN denotes spleen
T518 840-842 IN denotes In
T519 843-851 NN denotes response
T520 852-854 TO denotes to
T521 855-864 JJ denotes TOLL-like
T522 865-873 NN denotes receptor
T523 874-875 -LRB- denotes (
T524 875-878 NN denotes TLR
T525 878-879 -RRB- denotes )
T526 880-888 NN denotes agonists
T527 889-892 NN denotes LPS
T528 893-896 CC denotes and
T529 897-901 NN denotes poly
T530 902-903 NN denotes I
T531 903-904 -COLON- denotes :
T532 904-905 NN denotes C
T533 905-906 -COMMA- denotes ,
T534 907-915 VB denotes cultured
T535 916-920 NN denotes bone
T536 921-935 JJ denotes marrow-derived
T537 936-945 JJ denotes dendritic
T538 946-951 NN denotes cells
T539 952-953 -LRB- denotes (
T540 953-957 NN denotes BMDC
T541 957-958 -RRB- denotes )
T542 959-963 IN denotes from
T543 964-966 NN denotes WT
T544 967-970 CC denotes and
T545 971-977 JJ denotes mutant
T546 978-982 NN denotes mice
T547 983-992 VB denotes exhibited
T548 993-1000 JJ denotes similar
T549 1001-1010 NN denotes increases
T550 1011-1013 IN denotes in
T551 1014-1024 NN denotes expression
T552 1025-1027 IN denotes of
T553 1028-1042 JJ denotes co-stimulatory
T554 1043-1052 NN denotes molecules
T555 1053-1057 NN denotes CD40
T556 1057-1058 -COMMA- denotes ,
T557 1059-1063 NN denotes CD80
T558 1063-1064 -COMMA- denotes ,
T559 1065-1068 CC denotes and
T560 1069-1073 NN denotes CD86
T561 1075-1082 RB denotes However
T562 1082-1083 -COMMA- denotes ,
T563 1084-1094 VB denotes stimulated
T564 1095-1112 JJ denotes SHARPIN-deficient
T565 1113-1117 NN denotes BMDC
T566 1118-1121 VB denotes had
T567 1122-1129 VB denotes reduced
T568 1130-1143 NN denotes transcription
T569 1144-1147 CC denotes and
T570 1148-1157 NN denotes secretion
T571 1158-1160 IN denotes of
T572 1161-1177 JJ denotes pro-inflammatory
T573 1178-1187 NN denotes mediators
T574 1188-1191 NN denotes IL6
T575 1191-1192 -COMMA- denotes ,
T576 1193-1200 NN denotes IL12P70
T577 1200-1201 -COMMA- denotes ,
T578 1202-1207 NN denotes GMCSF
T579 1207-1208 -COMMA- denotes ,
T580 1209-1212 CC denotes and
T581 1213-1219 JJ denotes nitric
T582 1220-1225 NN denotes oxide
T583 1227-1233 JJ denotes Mutant
T584 1234-1238 NN denotes BMDC
T585 1239-1242 VB denotes had
T586 1243-1252 JJ denotes defective
T587 1253-1263 NN denotes activation
T588 1264-1266 IN denotes of
T589 1267-1272 NN denotes NF-κB
T590 1273-1282 NN denotes signaling
T591 1282-1283 -COMMA- denotes ,
T592 1284-1291 IN denotes whereas
T593 1292-1295 DT denotes the
T594 1296-1303 NN denotes MAPK1/3
T595 1304-1305 -LRB- denotes (
T596 1305-1311 NN denotes ERK1/2
T597 1311-1312 -RRB- denotes )
T598 1313-1316 CC denotes and
T599 1317-1332 NN denotes MAPK11/12/13/14
T600 1333-1334 -LRB- denotes (
T601 1334-1337 NN denotes p38
T602 1338-1341 NN denotes MAP
T603 1342-1348 NN denotes kinase
T604 1349-1357 NN denotes isoforms
T605 1357-1358 -RRB- denotes )
T606 1359-1362 CC denotes and
T607 1363-1367 NN denotes TBK1
T608 1368-1377 NN denotes signaling
T609 1378-1386 NN denotes pathways
T610 1387-1391 VB denotes were
T611 1392-1398 JJ denotes intact
T612 1400-1401 DT denotes A
T613 1402-1407 JJ denotes mixed
T614 1408-1418 NN denotes lymphocyte
T615 1419-1427 NN denotes reaction
T616 1428-1434 VB denotes showed
T617 1435-1439 IN denotes that
T618 1440-1446 JJ denotes mutant
T619 1447-1451 NN denotes BMDC
T620 1452-1456 RB denotes only
T621 1457-1464 VB denotes induced
T622 1465-1466 DT denotes a
T623 1467-1471 JJ denotes weak
T624 1472-1475 NN denotes Th1
T625 1476-1482 JJ denotes immune
T626 1483-1491 NN denotes response
T627 1492-1495 CC denotes but
T628 1496-1506 VB denotes stimulated
T629 1507-1516 VB denotes increased
T630 1517-1520 NN denotes Th2
T631 1521-1529 NN denotes cytokine
T632 1530-1540 NN denotes production
T633 1541-1545 IN denotes from
T634 1546-1556 JJ denotes allogeneic
T635 1557-1562 NN denotes naïve
T636 1563-1567 JJ denotes CD4+
T637 1568-1569 NN denotes T
T638 1570-1575 NN denotes cells
T639 1577-1579 IN denotes In
T640 1580-1590 NN denotes conclusion
T641 1590-1591 -COMMA- denotes ,
T642 1592-1596 NN denotes loss
T643 1597-1599 IN denotes of
T644 1600-1607 NN denotes Sharpin
T645 1608-1610 IN denotes in
T646 1611-1615 NN denotes mice
T647 1616-1629 RB denotes significantly
T648 1630-1637 VB denotes affects
T649 1638-1641 DT denotes the
T650 1642-1648 JJ denotes immune
T651 1649-1657 NN denotes function
T652 1658-1660 IN denotes of
T653 1661-1663 NN denotes DC
T654 1664-1667 CC denotes and
T655 1668-1672 DT denotes this
T656 1673-1676 MD denotes may
T657 1677-1686 RB denotes partially
T658 1687-1694 VB denotes account
T659 1695-1698 IN denotes for
T660 1699-1702 DT denotes the
T661 1703-1711 JJ denotes systemic
T662 1712-1724 NN denotes inflammation
T663 1725-1728 CC denotes and
T664 1729-1739 JJ denotes Th2-biased
T665 1740-1746 JJ denotes immune
T666 1747-1755 NN denotes response
T1486 1771-1778 NN denotes SHARPIN
T1487 1779-1782 VB denotes was
T1488 1783-1793 RB denotes originally
T1489 1794-1804 VB denotes identified
T1490 1805-1807 IN denotes in
T1491 1808-1821 JJ denotes post-synaptic
T1492 1822-1831 NN denotes densities
T1493 1832-1834 IN denotes of
T1494 1835-1845 JJ denotes excitatory
T1495 1846-1854 NN denotes synapses
T1496 1855-1857 IN denotes in
T1497 1858-1861 DT denotes the
T1498 1862-1867 NN denotes brain
T1499 1868-1870 IN denotes of
T1500 1871-1875 NN denotes rats
T1501 1876-1877 -LRB- denotes [
T1502 1877-1878 CD denotes 1
T1503 1878-1879 -RRB- denotes ]
T1504 1879-1880 -COMMA- denotes ,
T1505 1881-1884 CC denotes but
T1506 1885-1889 DT denotes this
T1507 1890-1897 NN denotes protein
T1508 1898-1900 VB denotes is
T1509 1901-1907 RB denotes widely
T1510 1908-1917 VB denotes expressed
T1511 1918-1920 IN denotes in
T1512 1921-1922 DT denotes a
T1513 1923-1930 NN denotes variety
T1514 1931-1933 IN denotes of
T1515 1934-1941 NN denotes tissues
T1516 1942-1943 -LRB- denotes [
T1517 1943-1944 CD denotes 2
T1518 1944-1945 -RRB- denotes ]
T1519 1947-1950 CD denotes Two
T1520 1951-1958 JJ denotes allelic
T1521 1958-1959 -COMMA- denotes ,
T1522 1960-1969 JJ denotes autosomal
T1523 1970-1979 JJ denotes recessive
T1524 1980-1989 NN denotes mutations
T1525 1990-1992 IN denotes in
T1526 1993-1996 DT denotes the
T1527 1997-2004 NN denotes Sharpin
T1528 2005-2009 NN denotes gene
T1529 2010-2018 VB denotes occurred
T1530 2019-2032 RB denotes spontaneously
T1531 2033-2035 IN denotes in
T1532 2036-2039 CD denotes two
T1533 2040-2046 JJ denotes inbred
T1534 2047-2054 NN denotes strains
T1535 2055-2057 IN denotes of
T1536 2058-2062 NN denotes mice
T1537 2062-2063 -COMMA- denotes ,
T1538 2064-2101 NN denotes C57B/KaLawRij-Sharpincpdm/Sharpincpdm
T1539 2102-2105 CC denotes and
T1540 2106-2150 NN denotes CBy.Ocb3/Dem-Sharpincpdm-Dem/Sharpincpdm-Dem
T1541 2150-2151 -COMMA- denotes ,
T1542 2152-2161 VB denotes resulting
T1543 2162-2164 IN denotes in
T1544 2165-2174 JJ denotes premature
T1545 2175-2186 NN denotes termination
T1546 2187-2189 IN denotes of
T1547 2190-2194 NN denotes mRNA
T1548 2195-2204 NN denotes synthesis
T1549 2205-2208 CC denotes and
T1550 2209-2216 NN denotes absence
T1551 2217-2219 IN denotes of
T1552 2220-2221 DT denotes a
T1553 2222-2232 JJ denotes functional
T1554 2233-2240 NN denotes protein
T1555 2241-2248 NN denotes product
T1556 2249-2250 -LRB- denotes [
T1557 2250-2251 CD denotes 2
T1558 2251-2252 -RRB- denotes ]
T1559 2254-2261 IN denotes Despite
T1560 2262-2271 JJ denotes different
T1561 2272-2279 JJ denotes genetic
T1562 2280-2291 NN denotes backgrounds
T1563 2291-2292 -COMMA- denotes ,
T1564 2293-2297 DT denotes both
T1565 2298-2307 NN denotes mutations
T1566 2308-2313 VB denotes cause
T1567 2314-2321 JJ denotes similar
T1568 2322-2334 JJ denotes inflammatory
T1569 2335-2342 NN denotes disease
T1570 2343-2347 IN denotes with
T1571 2348-2354 JJ denotes severe
T1572 2355-2362 JJ denotes chronic
T1573 2363-2374 JJ denotes progressive
T1574 2375-2385 NN denotes dermatitis
T1575 2386-2389 CC denotes and
T1576 2390-2399 JJ denotes defective
T1577 2400-2411 NN denotes development
T1578 2412-2414 IN denotes of
T1579 2415-2424 JJ denotes secondary
T1580 2425-2433 JJ denotes lymphoid
T1581 2434-2440 NN denotes organs
T1582 2441-2442 -LRB- denotes [
T1583 2442-2443 CD denotes 2
T1584 2443-2444 -RRB- denotes ]
T1585 2444-2445 NN denotes –
T1586 2445-2446 -LRB- denotes [
T1587 2446-2447 CD denotes 4
T1588 2447-2448 -RRB- denotes ]
T1589 2450-2453 DT denotes The
T1590 2454-2464 NN denotes dermatitis
T1591 2465-2472 VB denotes becomes
T1592 2473-2483 RB denotes clinically
T1593 2484-2492 JJ denotes apparent
T1594 2493-2495 IN denotes at
T1595 2496-2501 RB denotes about
T1596 2502-2506 CD denotes four
T1597 2507-2512 NN denotes weeks
T1598 2513-2515 IN denotes of
T1599 2516-2519 NN denotes age
T1600 2521-2526 EX denotes There
T1601 2527-2530 VB denotes are
T1602 2531-2544 NN denotes accumulations
T1603 2545-2547 IN denotes of
T1604 2548-2559 NN denotes eosinophils
T1605 2559-2560 -COMMA- denotes ,
T1606 2561-2572 NN denotes neutrophils
T1607 2573-2576 CC denotes and
T1608 2577-2588 NN denotes macrophages
T1609 2589-2591 IN denotes in
T1610 2592-2595 DT denotes the
T1611 2596-2600 NN denotes skin
T1612 2601-2603 IN denotes of
T1613 2604-2627 NNP denotes Sharpincpdm/Sharpincpdm
T1614 2628-2634 JJ denotes mutant
T1615 2635-2639 NN denotes mice
T1616 2640-2641 -LRB- denotes (
T1617 2641-2650 RB denotes hereafter
T1618 2651-2659 VB denotes referred
T1619 2660-2662 TO denotes to
T1620 2663-2665 IN denotes as
T1621 2666-2670 NN denotes cpdm
T1622 2671-2675 NN denotes mice
T1623 2675-2676 -RRB- denotes )
T1624 2677-2687 VB denotes associated
T1625 2688-2692 IN denotes with
T1626 2693-2702 VB denotes increased
T1627 2703-2713 NN denotes expression
T1628 2714-2716 IN denotes of
T1629 2717-2720 NN denotes Th2
T1630 2721-2730 NN denotes cytokines
T1631 2731-2733 IN denotes in
T1632 2734-2737 DT denotes the
T1633 2738-2742 NN denotes skin
T1634 2743-2746 CC denotes and
T1635 2747-2749 IN denotes in
T1636 2750-2753 DT denotes the
T1637 2754-2766 NN denotes supernatants
T1638 2767-2769 IN denotes of
T1639 2770-2779 VB denotes activated
T1640 2780-2791 NN denotes splenocytes
T1641 2792-2793 -LRB- denotes [
T1642 2793-2794 CD denotes 5
T1643 2794-2795 -RRB- denotes ]
T1644 2797-2800 DT denotes The
T1645 2801-2805 NN denotes mice
T1646 2806-2810 VB denotes have
T1647 2811-2813 DT denotes an
T1648 2814-2822 JJ denotes impaired
T1649 2823-2830 VB denotes delayed
T1650 2831-2835 NN denotes type
T1651 2836-2852 NN denotes hypersensitivity
T1652 2853-2861 NN denotes response
T1653 2862-2865 CC denotes and
T1654 2866-2875 VB denotes decreased
T1655 2876-2885 NN denotes secretion
T1656 2886-2888 IN denotes of
T1657 2889-2893 NN denotes IFNγ
T1658 2894-2895 -LRB- denotes [
T1659 2895-2896 CD denotes 5
T1660 2896-2897 -RRB- denotes ]
T1661 2897-2898 -COMMA- denotes ,
T1662 2899-2909 VB denotes indicating
T1663 2910-2911 DT denotes a
T1664 2912-2918 NN denotes defect
T1665 2919-2921 IN denotes in
T1666 2922-2925 NN denotes Th1
T1667 2926-2932 JJ denotes immune
T1668 2933-2942 NN denotes responses
T1669 2943-2946 CC denotes and
T1670 2947-2948 DT denotes a
T1671 2949-2953 NN denotes bias
T1672 2954-2961 IN denotes towards
T1673 2962-2963 DT denotes a
T1674 2964-2967 NN denotes Th2
T1675 2968-2974 JJ denotes immune
T1676 2975-2983 NN denotes response
T1677 2985-2993 JJ denotes Systemic
T1678 2994-3003 NN denotes treatment
T1679 3004-3006 IN denotes of
T1680 3007-3011 NN denotes cpdm
T1681 3012-3016 NN denotes mice
T1682 3017-3021 IN denotes with
T1683 3022-3033 JJ denotes recombinant
T1684 3034-3038 NN denotes IL12
T1685 3039-3045 VB denotes caused
T1686 3046-3054 JJ denotes complete
T1687 3055-3064 NN denotes remission
T1688 3065-3067 IN denotes of
T1689 3068-3071 DT denotes the
T1690 3072-3082 NN denotes dermatitis
T1691 3083-3084 -LRB- denotes [
T1692 3084-3085 CD denotes 5
T1693 3085-3086 -RRB- denotes ]
T1694 3088-3102 NN denotes Neutralization
T1695 3103-3105 IN denotes of
T1696 3106-3109 NN denotes IL5
T1697 3110-3112 IN denotes by
T1698 3113-3121 NN denotes antibody
T1699 3122-3131 NN denotes treatment
T1700 3132-3134 CC denotes or
T1701 3135-3142 NN denotes crosses
T1702 3143-3147 IN denotes with
T1703 3148-3161 JJ denotes IL5-deficient
T1704 3162-3166 NN denotes mice
T1705 3167-3174 VB denotes reduced
T1706 3175-3178 DT denotes the
T1707 3179-3185 NN denotes number
T1708 3186-3188 IN denotes of
T1709 3189-3200 VB denotes circulating
T1710 3201-3204 CC denotes and
T1711 3205-3214 JJ denotes cutaneous
T1712 3215-3226 NN denotes eosinophils
T1713 3226-3227 -COMMA- denotes ,
T1714 3228-3231 CC denotes but
T1715 3232-3238 VB denotes failed
T1716 3239-3241 TO denotes to
T1717 3242-3248 VB denotes reduce
T1718 3249-3252 DT denotes the
T1719 3253-3258 NN denotes onset
T1720 3259-3262 CC denotes and
T1721 3263-3271 NN denotes severity
T1722 3272-3274 IN denotes of
T1723 3275-3278 DT denotes the
T1724 3279-3289 NN denotes dermatitis
T1725 3290-3291 -LRB- denotes [
T1726 3291-3292 CD denotes 6
T1727 3292-3293 -RRB- denotes ]
T1728 3295-3303 RB denotes Recently
T1729 3303-3304 -COMMA- denotes ,
T1730 3305-3310 CD denotes three
T1731 3311-3322 JJ denotes independent
T1732 3323-3329 NN denotes groups
T1733 3330-3340 VB denotes identified
T1734 3341-3348 NN denotes SHARPIN
T1735 3349-3351 IN denotes as
T1736 3352-3354 DT denotes an
T1737 3355-3364 JJ denotes essential
T1738 3365-3374 NN denotes component
T1739 3375-3377 IN denotes of
T1740 3378-3381 DT denotes the
T1741 3382-3388 JJ denotes linear
T1742 3389-3398 NN denotes ubiquitin
T1743 3399-3404 NN denotes chain
T1744 3405-3413 NN denotes assembly
T1745 3414-3421 NN denotes complex
T1746 3422-3423 -LRB- denotes (
T1747 3423-3428 NN denotes LUBAC
T1748 3428-3429 -RRB- denotes )
T1749 3430-3434 WDT denotes that
T1750 3435-3444 VB denotes regulates
T1751 3445-3457 JJ denotes TNFα-induced
T1752 3458-3467 JJ denotes canonical
T1753 3468-3473 NN denotes NF-κB
T1754 3474-3483 NN denotes signaling
T1755 3484-3485 -LRB- denotes [
T1756 3485-3486 CD denotes 7
T1757 3486-3487 -RRB- denotes ]
T1758 3487-3488 NN denotes –
T1759 3488-3489 -LRB- denotes [
T1760 3489-3490 CD denotes 9
T1761 3490-3491 -RRB- denotes ]
T1762 3493-3510 JJ denotes SHARPIN-deficient
T1763 3511-3516 NN denotes mouse
T1764 3517-3526 JJ denotes embryonic
T1765 3527-3537 NN denotes fibroblast
T1766 3538-3539 -LRB- denotes (
T1767 3539-3542 NN denotes MEF
T1768 3542-3543 -RRB- denotes )
T1769 3544-3548 VB denotes were
T1770 3549-3559 VB denotes sensitized
T1771 3560-3562 TO denotes to
T1772 3563-3575 JJ denotes TNFα-induced
T1773 3576-3585 NN denotes apoptosis
T1774 3586-3589 CC denotes and
T1775 3590-3594 NN denotes cell
T1776 3595-3600 NN denotes death
T1777 3601-3604 VB denotes was
T1778 3605-3615 VB denotes implicated
T1779 3616-3618 IN denotes as
T1780 3619-3620 DT denotes a
T1781 3621-3627 NN denotes factor
T1782 3628-3630 IN denotes in
T1783 3631-3634 DT denotes the
T1784 3635-3645 NN denotes dermatitis
T1785 3646-3648 IN denotes of
T1786 3649-3653 NN denotes cpdm
T1787 3654-3658 NN denotes mice
T1788 3659-3660 -LRB- denotes [
T1789 3660-3661 CD denotes 7
T1790 3661-3662 -RRB- denotes ]
T1791 3662-3663 NN denotes –
T1792 3663-3664 -LRB- denotes [
T1793 3664-3665 CD denotes 9
T1794 3665-3666 -RRB- denotes ]
T1795 3668-3677 JJ denotes Dendritic
T1796 3678-3683 NN denotes cells
T1797 3684-3685 -LRB- denotes (
T1798 3685-3687 NN denotes DC
T1799 3687-3688 -RRB- denotes )
T1800 3689-3693 VB denotes have
T1801 3694-3695 DT denotes a
T1802 3696-3704 NN denotes sentinel
T1803 3705-3709 NN denotes role
T1804 3710-3712 IN denotes in
T1805 3713-3720 VB denotes sensing
T1806 3721-3729 NN denotes pathogen
T1807 3730-3732 CC denotes or
T1808 3733-3739 NN denotes danger
T1809 3740-3747 NN denotes signals
T1810 3748-3751 CC denotes and
T1811 3752-3760 NN denotes initiate
T1812 3761-3764 CC denotes and
T1813 3765-3771 JJ denotes direct
T1814 3772-3782 NN denotes activation
T1815 3783-3785 IN denotes of
T1816 3786-3789 DT denotes the
T1817 3790-3798 JJ denotes adaptive
T1818 3799-3805 JJ denotes immune
T1819 3806-3814 NN denotes response
T1820 3815-3816 -LRB- denotes [
T1821 3816-3818 CD denotes 10
T1822 3818-3819 -RRB- denotes ]
T1823 3821-3830 VB denotes Activated
T1824 3831-3834 CC denotes and
T1825 3835-3841 JJ denotes mature
T1826 3842-3844 NN denotes DC
T1827 3845-3848 MD denotes can
T1828 3849-3854 VB denotes carry
T1829 3855-3864 VB denotes processed
T1830 3865-3874 JJ denotes antigenic
T1831 3875-3883 NN denotes peptides
T1832 3883-3884 -COMMA- denotes ,
T1833 3885-3892 VB denotes migrate
T1834 3893-3895 TO denotes to
T1835 3896-3904 JJ denotes lymphoid
T1836 3905-3911 NN denotes organs
T1837 3911-3912 -COMMA- denotes ,
T1838 3913-3916 CC denotes and
T1839 3917-3923 VB denotes induce
T1840 3924-3939 JJ denotes T-cell-mediated
T1841 3940-3946 JJ denotes immune
T1842 3947-3956 NN denotes responses
T1843 3957-3959 CC denotes or
T1844 3960-3969 NN denotes tolerance
T1845 3971-3973 NN denotes DC
T1846 3974-3980 VB denotes direct
T1847 3981-3984 DT denotes the
T1848 3985-4000 NN denotes differentiation
T1849 4001-4003 IN denotes of
T1850 4004-4008 JJ denotes CD4+
T1851 4009-4010 NN denotes T
T1852 4011-4016 NN denotes cells
T1853 4016-4017 -COMMA- denotes ,
T1854 4018-4021 CC denotes and
T1855 4022-4027 RB denotes hence
T1856 4028-4031 DT denotes the
T1857 4032-4036 NN denotes type
T1858 4037-4039 IN denotes of
T1859 4040-4046 JJ denotes immune
T1860 4047-4055 NN denotes response
T1861 4055-4056 -COMMA- denotes ,
T1862 4057-4064 IN denotes through
T1863 4065-4068 DT denotes the
T1864 4069-4078 JJ denotes selective
T1865 4079-4088 NN denotes secretion
T1866 4089-4091 IN denotes of
T1867 4092-4101 NN denotes cytokines
T1868 4103-4105 PRP denotes We
T1869 4106-4118 VB denotes hypothesized
T1870 4119-4123 IN denotes that
T1871 4124-4133 JJ denotes defective
T1872 4134-4142 NN denotes cytokine
T1873 4143-4152 NN denotes secretion
T1874 4153-4155 IN denotes by
T1875 4156-4158 NN denotes DC
T1876 4159-4170 VB denotes contributed
T1877 4171-4173 TO denotes to
T1878 4174-4177 DT denotes the
T1879 4178-4188 JJ denotes Th2-biased
T1880 4189-4201 JJ denotes inflammatory
T1881 4202-4211 NN denotes phenotype
T1882 4212-4214 IN denotes in
T1883 4215-4232 JJ denotes SHARPIN-deficient
T1884 4233-4237 NN denotes mice
T1885 4239-4242 DT denotes The
T1886 4243-4250 NN denotes studies
T1887 4251-4259 VB denotes reported
T1888 4260-4264 RB denotes here
T1889 4265-4270 VB denotes found
T1890 4271-4275 IN denotes that
T1891 4276-4280 NN denotes lack
T1892 4281-4283 IN denotes of
T1893 4284-4291 NN denotes SHARPIN
T1894 4292-4299 NN denotes protein
T1895 4300-4302 IN denotes in
T1896 4303-4307 NN denotes BMDC
T1897 4308-4314 VB denotes caused
T1898 4315-4324 JJ denotes defective
T1899 4325-4335 NN denotes expression
T1900 4336-4338 IN denotes of
T1901 4339-4355 JJ denotes pro-inflammatory
T1902 4356-4365 NN denotes mediators
T1903 4366-4369 CC denotes and
T1904 4370-4378 JJ denotes impaired
T1905 4379-4384 NN denotes NF-κB
T1906 4385-4395 NN denotes activation
T1907 4396-4400 IN denotes upon
T1908 4401-4407 NN denotes ligand
T1909 4408-4419 NN denotes stimulation
T1910 4421-4424 DT denotes The
T1911 4425-4432 NN denotes ability
T1912 4433-4435 IN denotes of
T1913 4436-4440 NN denotes cpdm
T1914 4441-4445 NN denotes BMDC
T1915 4446-4448 TO denotes to
T1916 4449-4458 VB denotes stimulate
T1917 4459-4462 NN denotes Th1
T1918 4463-4471 NN denotes cytokine
T1919 4472-4482 NN denotes production
T1920 4483-4485 IN denotes in
T1921 4486-4496 JJ denotes allogeneic
T1922 4497-4501 JJ denotes CD4+
T1923 4502-4503 NN denotes T
T1924 4504-4509 NN denotes cells
T1925 4510-4513 VB denotes was
T1926 4514-4525 VB denotes compromised
T1927 4527-4532 VB denotes Taken
T1928 4533-4541 RB denotes together
T1929 4541-4542 -COMMA- denotes ,
T1930 4543-4548 DT denotes these
T1931 4549-4556 NN denotes results
T1932 4557-4563 VB denotes reveal
T1933 4564-4568 IN denotes that
T1934 4569-4576 NN denotes SHARPIN
T1935 4577-4579 VB denotes is
T1936 4580-4581 DT denotes a
T1937 4582-4587 JJ denotes novel
T1938 4588-4598 JJ denotes regulatory
T1939 4599-4607 NN denotes molecule
T1940 4608-4610 IN denotes in
T1941 4611-4613 NN denotes DC
T1942 4614-4621 NN denotes biology
T1943 4622-4625 CC denotes and
T1944 4626-4633 VB denotes suggest
T1945 4634-4638 IN denotes that
T1946 4639-4642 DT denotes the
T1947 4643-4655 VB denotes dysregulated
T1948 4656-4664 NN denotes function
T1949 4665-4667 IN denotes of
T1950 4668-4685 JJ denotes SHARPIN-deficient
T1951 4686-4688 NN denotes DC
T1952 4689-4694 VB denotes plays
T1953 4695-4696 DT denotes a
T1954 4697-4701 NN denotes role
T1955 4702-4704 IN denotes in
T1956 4705-4708 DT denotes the
T1957 4709-4713 NN denotes cpdm
T1958 4714-4723 NN denotes phenotype
T2461 4735-4751 NN denotes Characterization
T2462 4752-4754 IN denotes of
T2463 4755-4762 NNP denotes Sharpin
T2464 4764-4773 NN denotes Orthologs
T2465 4774-4776 IN denotes of
T2466 4777-4784 NN denotes SHARPIN
T2467 4785-4792 NN denotes protein
T2468 4793-4796 VB denotes are
T2469 4797-4802 VB denotes found
T2470 4803-4805 IN denotes in
T2471 4806-4813 JJ denotes various
T2472 4814-4821 NN denotes species
T2473 4821-4822 -COMMA- denotes ,
T2474 4823-4832 VB denotes including
T2475 4833-4838 JJ denotes human
T2476 4838-4839 -COMMA- denotes ,
T2477 4840-4845 NN denotes mouse
T2478 4846-4849 CC denotes and
T2479 4850-4853 NN denotes rat
T2480 4855-4860 NN denotes Motif
T2481 4861-4871 NN denotes prediction
T2482 4872-4883 NN denotes programming
T2483 4883-4884 -COMMA- denotes ,
T2484 4885-4890 VB denotes using
T2485 4891-4896 NN denotes COILS
T2486 4897-4898 -LRB- denotes [
T2487 4898-4900 CD denotes 11
T2488 4900-4901 -RRB- denotes ]
T2489 4902-4905 CC denotes and
T2490 4906-4915 NNP denotes MotifScan
T2491 4916-4917 -LRB- denotes [
T2492 4917-4919 CD denotes 12
T2493 4919-4920 -RRB- denotes ]
T2494 4920-4921 -COMMA- denotes ,
T2495 4922-4930 VB denotes suggests
T2496 4931-4935 IN denotes that
T2497 4936-4943 NN denotes SHARPIN
T2498 4944-4952 VB denotes contains
T2499 4953-4954 DT denotes a
T2500 4955-4966 NN denotes coiled-coil
T2501 4967-4968 -LRB- denotes (
T2502 4968-4970 NN denotes CC
T2503 4970-4971 -RRB- denotes )
T2504 4972-4978 NN denotes domain
T2505 4978-4979 -COMMA- denotes ,
T2506 4980-4981 DT denotes a
T2507 4982-4996 JJ denotes ubiquitin-like
T2508 4997-4998 -LRB- denotes (
T2509 4998-5001 NN denotes UBL
T2510 5001-5002 -RRB- denotes )
T2511 5003-5009 NN denotes domain
T2512 5009-5010 -COMMA- denotes ,
T2513 5011-5014 CC denotes and
T2514 5015-5016 DT denotes a
T2515 5017-5028 JJ denotes zinc-finger
T2516 5029-5040 JJ denotes Ran-binding
T2517 5041-5048 NN denotes protein
T2518 5049-5050 CD denotes 2
T2519 5051-5052 -LRB- denotes (
T2520 5052-5057 NN denotes ZFRBP
T2521 5057-5058 -RRB- denotes )
T2522 5059-5065 NN denotes domain
T2523 5067-5072 DT denotes These
T2524 5073-5083 JJ denotes functional
T2525 5084-5090 NN denotes motifs
T2526 5091-5101 VB denotes constitute
T2527 5102-5109 JJ denotes similar
T2528 5110-5116 NN denotes domain
T2529 5117-5125 NN denotes profiles
T2530 5126-5130 WDT denotes that
T2531 5131-5134 VB denotes are
T2532 5135-5142 JJ denotes present
T2533 5143-5145 IN denotes in
T2534 5146-5149 DT denotes the
T2535 5150-5157 NN denotes SHARPIN
T2536 5158-5165 NN denotes protein
T2537 5166-5168 IN denotes of
T2538 5169-5172 DT denotes all
T2539 5173-5178 CD denotes three
T2540 5179-5186 NN denotes origins
T2541 5187-5188 -LRB- denotes (
T2542 5188-5192 NNP denotes Fig.
T2543 5193-5195 NN denotes 1A
T2544 5195-5196 -RRB- denotes )
T2545 5196-5197 -COMMA- denotes ,
T2546 5198-5208 VB denotes suggesting
T2547 5209-5213 IN denotes that
T2548 5214-5221 NN denotes SHARPIN
T2549 5222-5228 NN denotes exerts
T2550 5229-5235 RB denotes highly
T2551 5236-5245 VB denotes conserved
T2552 5246-5255 NN denotes functions
T2553 5256-5262 IN denotes across
T2554 5263-5270 NN denotes species
T2555 5272-5283 JJ denotes Spontaneous
T2556 5284-5293 NN denotes mutations
T2557 5294-5296 IN denotes in
T2558 5297-5300 DT denotes the
T2559 5301-5306 NN denotes mouse
T2560 5307-5314 NN denotes Sharpin
T2561 5315-5319 NN denotes gene
T2562 5320-5327 VB denotes results
T2563 5328-5330 IN denotes in
T2564 5331-5332 DT denotes a
T2565 5333-5340 JJ denotes complex
T2566 5341-5353 JJ denotes inflammatory
T2567 5354-5363 NN denotes phenotype
T2568 5364-5377 VB denotes characterized
T2569 5378-5380 IN denotes by
T2570 5381-5387 JJ denotes severe
T2571 5388-5398 NN denotes dermatitis
T2572 5399-5400 -LRB- denotes (
T2573 5400-5404 NN denotes Fig.
T2574 5405-5407 NN denotes 1B
T2575 5407-5408 -RRB- denotes )
T2576 5408-5409 -COMMA- denotes ,
T2577 5410-5418 JJ denotes systemic
T2578 5419-5431 NN denotes inflammation
T2579 5432-5435 CC denotes and
T2580 5436-5438 DT denotes an
T2581 5439-5447 JJ denotes enlarged
T2582 5448-5454 NN denotes spleen
T2583 5455-5456 -LRB- denotes (
T2584 5456-5460 NN denotes Fig.
T2585 5461-5463 NN denotes 1C
T2586 5463-5464 -RRB- denotes )
T2587 5465-5471 VB denotes caused
T2588 5472-5474 IN denotes by
T2589 5475-5489 JJ denotes extramedullary
T2590 5490-5503 NN denotes hematopoiesis
T2591 5504-5505 -LRB- denotes [
T2592 5505-5506 CD denotes 3
T2593 5506-5507 -RRB- denotes ]
T2594 5509-5512 DT denotes The
T2595 5513-5523 JJ denotes endogenous
T2596 5524-5534 NN denotes expression
T2597 5535-5537 IN denotes of
T2598 5538-5545 NN denotes Sharpin
T2599 5546-5550 NN denotes mRNA
T2600 5551-5553 IN denotes in
T2601 5554-5558 NN denotes BMDC
T2602 5559-5562 VB denotes was
T2603 5563-5573 VB denotes determined
T2604 5574-5576 IN denotes by
T2605 5577-5589 JJ denotes quantitative
T2606 5590-5594 JJ denotes real
T2607 5595-5603 NN denotes time-PCR
T2608 5604-5605 -LRB- denotes (
T2609 5605-5612 NN denotes qRT-PCR
T2610 5612-5613 -RRB- denotes )
T2611 5614-5623 VB denotes following
T2612 5624-5631 NN denotes culture
T2613 5632-5634 IN denotes in
T2614 5635-5641 NN denotes medium
T2615 5642-5646 RB denotes only
T2616 5647-5649 CC denotes or
T2617 5650-5655 IN denotes after
T2618 5656-5667 NN denotes stimulation
T2619 5668-5672 IN denotes with
T2620 5673-5676 NN denotes LPS
T2621 5678-5685 NN denotes Sharpin
T2622 5686-5690 NN denotes mRNA
T2623 5691-5694 VB denotes was
T2624 5695-5702 JJ denotes present
T2625 5703-5705 IN denotes in
T2626 5706-5710 NN denotes BMDC
T2627 5711-5720 VB denotes generated
T2628 5721-5725 IN denotes from
T2629 5726-5728 JJ denotes WT
T2630 5729-5733 NN denotes mice
T2631 5734-5735 -LRB- denotes (
T2632 5735-5739 NNP denotes Fig.
T2633 5740-5742 NN denotes 1D
T2634 5742-5743 -RRB- denotes )
T2635 5744-5747 CC denotes and
T2636 5748-5751 PRP-DOLLAR- denotes its
T2637 5752-5757 NN denotes level
T2638 5758-5761 VB denotes was
T2639 5762-5770 RB denotes modestly
T2640 5771-5780 VB denotes decreased
T2641 5781-5783 IN denotes by
T2642 5784-5787 NN denotes LPS
T2643 5788-5799 NN denotes stimulation
T2644 5801-5806 EX denotes There
T2645 5807-5810 VB denotes was
T2646 5811-5812 DT denotes a
T2647 5813-5824 JJ denotes significant
T2648 5825-5834 NN denotes reduction
T2649 5835-5837 IN denotes of
T2650 5838-5845 NN denotes Sharpin
T2651 5846-5850 NN denotes mRNA
T2652 5851-5852 -LRB- denotes (
T2653 5852-5860 JJ denotes 6–7-fold
T2654 5860-5861 -RRB- denotes )
T2655 5862-5864 IN denotes in
T2656 5865-5869 NN denotes BMDC
T2657 5870-5879 VB denotes generated
T2658 5880-5884 IN denotes from
T2659 5885-5889 NN denotes cpdm
T2660 5890-5894 NN denotes mice
T2661 5896-5908 NN denotes Transfection
T2662 5909-5911 IN denotes of
T2663 5912-5923 JJ denotes Flag-tagged
T2664 5924-5931 NNP denotes Sharpin
T2665 5932-5934 IN denotes in
T2666 5935-5946 NN denotes fibroblasts
T2667 5947-5948 -LRB- denotes (
T2668 5948-5954 NN denotes NIH3T3
T2669 5954-5955 -RRB- denotes )
T2670 5956-5959 CC denotes and
T2671 5960-5971 NN denotes macrophages
T2672 5972-5973 -LRB- denotes (
T2673 5973-5981 NN denotes RAW264.7
T2674 5981-5982 -RRB- denotes )
T2675 5983-5992 VB denotes indicated
T2676 5993-6004 JJ denotes cytoplasmic
T2677 6005-6017 NN denotes localization
T2678 6018-6020 IN denotes of
T2679 6021-6024 DT denotes the
T2680 6025-6032 NN denotes SHARPIN
T2681 6033-6040 NN denotes protein
T2682 6041-6042 -LRB- denotes (
T2683 6042-6046 NN denotes Fig.
T2684 6047-6049 NN denotes 1E
T2685 6049-6050 -RRB- denotes )
T3281 7229-7240 NN denotes Phenotyping
T3282 7241-7248 JJ denotes splenic
T3283 7249-7251 NN denotes DC
T3284 7252-7255 CC denotes and
T3285 7256-7260 NN denotes BMDC
T3286 7261-7265 IN denotes from
T3287 7266-7268 NN denotes WT
T3288 7269-7272 CC denotes and
T3289 7273-7277 NN denotes cpdm
T3290 7278-7282 NN denotes mice
T3291 7283-7285 NN denotes DC
T3292 7286-7289 VB denotes are
T3293 7290-7303 JJ denotes heterogeneous
T3294 7304-7307 CC denotes and
T3295 7308-7311 MD denotes can
T3296 7312-7314 VB denotes be
T3297 7315-7326 VB denotes categorized
T3298 7327-7331 IN denotes into
T3299 7332-7340 JJ denotes multiple
T3300 7341-7349 NN denotes subtypes
T3301 7350-7355 VB denotes based
T3302 7356-7358 IN denotes on
T3303 7359-7366 NN denotes surface
T3304 7367-7374 NN denotes markers
T3305 7375-7376 -LRB- denotes [
T3306 7376-7378 CD denotes 13
T3307 7378-7379 -RRB- denotes ]
T3308 7381-7383 TO denotes To
T3309 7384-7393 VB denotes determine
T3310 7394-7396 IN denotes if
T3311 7397-7400 DT denotes the
T3312 7401-7408 NNP denotes Sharpin
T3313 7409-7417 NN denotes mutation
T3314 7418-7425 VB denotes affects
T3315 7426-7428 NN denotes DC
T3316 7429-7440 NN denotes development
T3317 7441-7443 IN denotes in
T3318 7444-7452 JJ denotes lymphoid
T3319 7453-7460 NN denotes tissues
T3320 7460-7461 -COMMA- denotes ,
T3321 7462-7467 NN denotes mouse
T3322 7468-7475 NN denotes spleens
T3323 7476-7480 VB denotes were
T3324 7481-7489 VB denotes examined
T3325 7490-7493 IN denotes for
T3326 7494-7497 DT denotes the
T3327 7498-7510 NN denotes distribution
T3328 7511-7513 IN denotes of
T3329 7514-7526 JJ denotes conventional
T3330 7527-7529 NN denotes DC
T3331 7530-7531 -LRB- denotes (
T3332 7531-7534 NN denotes cDC
T3333 7534-7535 -COLON- denotes ;
T3334 7536-7547 JJ denotes CD11c+CD8α+
T3335 7548-7551 CC denotes and
T3336 7552-7563 NN denotes CD11c+CD8α−
T3337 7563-7564 -RRB- denotes )
T3338 7565-7566 -LRB- denotes [
T3339 7566-7568 CD denotes 13
T3340 7568-7569 -RRB- denotes ]
T3341 7570-7573 CC denotes and
T3342 7574-7586 JJ denotes plasmacytoid
T3343 7587-7589 NN denotes DC
T3344 7590-7591 -LRB- denotes (
T3345 7591-7594 NN denotes pDC
T3346 7594-7595 -COLON- denotes ;
T3347 7596-7609 JJ denotes CD11c−PDCA-1+
T3348 7609-7610 -RRB- denotes )
T3349 7611-7612 -LRB- denotes [
T3350 7612-7614 CD denotes 14
T3351 7614-7615 -RRB- denotes ]
T3352 7617-7620 DT denotes The
T3353 7621-7632 NN denotes percentages
T3354 7633-7636 IN denotes for
T3355 7637-7644 JJ denotes splenic
T3356 7645-7648 NN denotes cDC
T3357 7649-7652 CC denotes and
T3358 7653-7656 NN denotes pDC
T3359 7657-7661 VB denotes were
T3360 7662-7666 DT denotes both
T3361 7667-7674 VB denotes reduced
T3362 7675-7677 IN denotes in
T3363 7678-7682 NN denotes cpdm
T3364 7683-7687 NN denotes mice
T3365 7688-7692 WRB denotes when
T3366 7693-7701 VB denotes compared
T3367 7702-7706 IN denotes with
T3368 7707-7709 JJ denotes WT
T3369 7710-7718 NN denotes controls
T3370 7719-7720 -LRB- denotes (
T3371 7720-7724 NNP denotes Fig.
T3372 7725-7727 NN denotes 2A
T3373 7727-7728 -RRB- denotes )
T3374 7730-7737 RB denotes However
T3375 7737-7738 -COMMA- denotes ,
T3376 7739-7743 WRB denotes when
T3377 7744-7749 VB denotes gated
T3378 7750-7752 IN denotes on
T3379 7753-7759 JJ denotes CD11c+
T3380 7760-7763 NN denotes cDC
T3381 7763-7764 -COMMA- denotes ,
T3382 7765-7768 DT denotes the
T3383 7769-7780 NN denotes percentages
T3384 7781-7783 IN denotes of
T3385 7784-7789 JJ denotes CD8α+
T3386 7790-7793 CC denotes and
T3387 7794-7799 NN denotes CD8α−
T3388 7800-7805 NN denotes cells
T3389 7806-7810 VB denotes were
T3390 7811-7814 RB denotes not
T3391 7815-7823 VB denotes affected
T3392 7824-7826 IN denotes by
T3393 7827-7834 NN denotes SHARPIN
T3394 7835-7845 NN denotes deficiency
T3395 7846-7847 -LRB- denotes (
T3396 7847-7851 NN denotes Fig.
T3397 7852-7854 NN denotes 2A
T3398 7854-7855 -RRB- denotes )
T3399 7857-7862 IN denotes Since
T3400 7863-7866 DT denotes the
T3401 7867-7873 NN denotes spleen
T3402 7874-7876 IN denotes of
T3403 7877-7881 NN denotes cpdm
T3404 7882-7886 NN denotes mice
T3405 7887-7889 VB denotes is
T3406 7890-7898 RB denotes markedly
T3407 7899-7907 VB denotes enlarged
T3408 7908-7911 CC denotes and
T3409 7912-7920 VB denotes contains
T3410 7921-7926 CD denotes three
T3411 7927-7932 NN denotes times
T3412 7933-7935 IN denotes as
T3413 7936-7940 JJ denotes many
T3414 7941-7946 NN denotes cells
T3415 7947-7948 -LRB- denotes (
T3416 7948-7952 NN denotes Fig.
T3417 7953-7955 NN denotes 1C
T3418 7955-7956 -RRB- denotes )
T3419 7956-7957 -COMMA- denotes ,
T3420 7958-7961 DT denotes the
T3421 7962-7971 JJ denotes different
T3422 7972-7983 NN denotes percentages
T3423 7984-7986 IN denotes of
T3424 7987-7994 JJ denotes splenic
T3425 7995-7998 NN denotes cDC
T3426 7999-8002 CC denotes and
T3427 8003-8006 NN denotes pDC
T3428 8007-8014 IN denotes between
T3429 8015-8017 NN denotes WT
T3430 8018-8021 CC denotes and
T3431 8022-8028 JJ denotes mutant
T3432 8029-8033 NN denotes mice
T3433 8034-8041 VB denotes reflect
T3434 8042-8045 DT denotes the
T3435 8046-8055 VB denotes increased
T3436 8056-8062 NN denotes number
T3437 8063-8065 IN denotes of
T3438 8066-8071 JJ denotes total
T3439 8072-8078 NN denotes spleen
T3440 8079-8084 NN denotes cells
T3441 8085-8091 RB denotes rather
T3442 8092-8096 IN denotes than
T3443 8097-8098 DT denotes a
T3444 8099-8108 NN denotes reduction
T3445 8109-8111 IN denotes in
T3446 8112-8115 NN denotes cDC
T3447 8116-8119 CC denotes and
T3448 8120-8123 NN denotes pDC
T3449 8124-8131 NN denotes numbers
T3450 8133-8138 DT denotes These
T3451 8139-8143 NN denotes data
T3452 8144-8152 VB denotes indicate
T3453 8153-8157 IN denotes that
T3454 8158-8161 DT denotes the
T3455 8162-8169 NNP denotes Sharpin
T3456 8170-8178 NN denotes mutation
T3457 8179-8183 VB denotes does
T3458 8184-8187 RB denotes not
T3459 8188-8194 VB denotes affect
T3460 8195-8198 DT denotes the
T3461 8199-8211 NN denotes distribution
T3462 8212-8214 IN denotes of
T3463 8215-8218 DT denotes the
T3464 8219-8227 VB denotes examined
T3465 8228-8230 NN denotes DC
T3466 8231-8238 NN denotes subsets
T3467 8239-8241 IN denotes in
T3468 8242-8245 DT denotes the
T3469 8246-8252 NN denotes spleen
T3470 9576-9580 NN denotes BMDC
T3471 9581-9585 IN denotes from
T3472 9586-9588 FW denotes in
T3473 9589-9594 FW denotes vitro
T3474 9595-9603 NN denotes cultures
T3475 9604-9616 RB denotes functionally
T3476 9617-9625 VB denotes resemble
T3477 9626-9638 JJ denotes non-lymphoid
T3478 9639-9645 NN denotes tissue
T3479 9646-9648 NN denotes DC
T3480 9649-9652 CC denotes and
T3481 9653-9669 JJ denotes monocyte-derived
T3482 9670-9682 JJ denotes inflammatory
T3483 9683-9685 NN denotes DC
T3484 9686-9687 -LRB- denotes [
T3485 9687-9689 CD denotes 15
T3486 9689-9690 -RRB- denotes ]
T3487 9690-9691 -COMMA- denotes ,
T3488 9692-9693 -LRB- denotes [
T3489 9693-9695 CD denotes 16
T3490 9695-9696 -RRB- denotes ]
T3491 9698-9701 DT denotes The
T3492 9702-9708 NN denotes yields
T3493 9709-9711 IN denotes of
T3494 9712-9716 NN denotes BMDC
T3495 9717-9721 IN denotes from
T3496 9722-9724 NN denotes WT
T3497 9725-9728 CC denotes and
T3498 9729-9733 NN denotes cpdm
T3499 9734-9738 NN denotes mice
T3500 9739-9743 VB denotes were
T3501 9744-9751 JJ denotes similar
T3502 9753-9757 NN denotes BMDC
T3503 9758-9762 VB denotes were
T3504 9763-9769 JJ denotes CD11c+
T3505 9770-9773 CC denotes and
T3506 9774-9777 NN denotes MHC
T3507 9778-9781 JJ denotes II+
T3508 9782-9786 IN denotes with
T3509 9787-9790 JJ denotes low
T3510 9791-9801 NN denotes expression
T3511 9802-9804 IN denotes of
T3512 9805-9819 JJ denotes co-stimulatory
T3513 9820-9829 NN denotes molecules
T3514 9830-9834 NN denotes CD40
T3515 9834-9835 -COMMA- denotes ,
T3516 9836-9840 NN denotes CD80
T3517 9840-9841 -COMMA- denotes ,
T3518 9842-9845 CC denotes and
T3519 9846-9850 NN denotes CD86
T3520 9852-9855 DT denotes The
T3521 9856-9860 NN denotes TLR3
T3522 9861-9867 NN denotes ligand
T3523 9868-9872 NN denotes poly
T3524 9873-9874 NN denotes I
T3525 9874-9875 -COLON- denotes :
T3526 9875-9876 NN denotes C
T3527 9877-9880 CC denotes and
T3528 9881-9884 DT denotes the
T3529 9885-9889 NN denotes TLR4
T3530 9890-9896 NN denotes ligand
T3531 9897-9900 NN denotes LPS
T3532 9901-9905 DT denotes each
T3533 9906-9914 VB denotes activate
T3534 9915-9926 VB denotes overlapping
T3535 9927-9930 CC denotes but
T3536 9931-9940 JJ denotes different
T3537 9941-9950 NN denotes signaling
T3538 9951-9959 NN denotes pathways
T3539 9960-9963 CC denotes and
T3540 9964-9968 VB denotes were
T3541 9969-9973 VB denotes used
T3542 9974-9976 TO denotes to
T3543 9977-9983 VB denotes induce
T3544 9984-9986 NN denotes DC
T3545 9987-9997 NN denotes maturation
T3546 9998-9999 -LRB- denotes [
T3547 9999-10001 CD denotes 17
T3548 10001-10002 -RRB- denotes ]
T3549 10002-10003 -COMMA- denotes ,
T3550 10004-10005 -LRB- denotes [
T3551 10005-10007 CD denotes 18
T3552 10007-10008 -RRB- denotes ]
T3553 10010-10020 NN denotes Incubation
T3554 10021-10025 IN denotes with
T3555 10026-10029 DT denotes the
T3556 10030-10033 NN denotes TLR
T3557 10034-10042 NN denotes agonists
T3558 10043-10046 IN denotes for
T3559 10047-10049 CD denotes 24
T3560 10050-10055 NN denotes hours
T3561 10056-10064 VB denotes resulted
T3562 10065-10067 IN denotes in
T3563 10068-10077 VB denotes increased
T3564 10078-10088 NN denotes expression
T3565 10089-10091 IN denotes of
T3566 10092-10096 NN denotes CD40
T3567 10096-10097 -COMMA- denotes ,
T3568 10098-10102 NN denotes CD80
T3569 10102-10103 -COMMA- denotes ,
T3570 10104-10107 CC denotes and
T3571 10108-10112 NN denotes CD86
T3572 10113-10115 IN denotes on
T3573 10116-10120 NN denotes BMDC
T3574 10120-10121 -COLON- denotes ;
T3575 10122-10129 RB denotes however
T3576 10129-10130 -COMMA- denotes ,
T3577 10131-10136 EX denotes there
T3578 10137-10140 VB denotes was
T3579 10141-10143 DT denotes no
T3580 10144-10154 NN denotes difference
T3581 10155-10157 IN denotes in
T3582 10158-10161 DT denotes the
T3583 10162-10172 NN denotes expression
T3584 10173-10179 NN denotes levels
T3585 10180-10182 IN denotes of
T3586 10183-10188 DT denotes these
T3587 10189-10196 NN denotes markers
T3588 10197-10204 IN denotes between
T3589 10205-10207 NN denotes WT
T3590 10208-10211 CC denotes and
T3591 10212-10216 NN denotes cpdm
T3592 10217-10221 NN denotes BMDC
T3593 10222-10223 -LRB- denotes (
T3594 10223-10227 NN denotes Fig.
T3595 10228-10230 NN denotes 2B
T3596 10230-10231 -RRB- denotes )
T3597 10233-10237 RB denotes Thus
T3598 10237-10238 -COMMA- denotes ,
T3599 10239-10246 NN denotes SHARPIN
T3600 10247-10257 NN denotes deficiency
T3601 10258-10261 VB denotes did
T3602 10262-10265 RB denotes not
T3603 10266-10275 VB denotes influence
T3604 10276-10279 DT denotes the
T3605 10280-10290 NN denotes expression
T3606 10291-10293 IN denotes of
T3607 10294-10308 JJ denotes co-stimulatory
T3608 10309-10318 NN denotes molecules
T3609 10319-10321 IN denotes by
T3610 10322-10326 NN denotes BMDC
T4014 10329-10339 NN denotes Production
T4015 10340-10342 IN denotes of
T4016 10343-10358 JJ denotes proinflammatory
T4017 10359-10368 NN denotes mediators
T4018 10369-10371 IN denotes by
T4019 10372-10376 NN denotes cpdm
T4020 10377-10381 NN denotes BMDC
T4021 10382-10384 VB denotes is
T4022 10385-10393 VB denotes impaired
T4023 10394-10404 NN denotes Incubation
T4024 10405-10409 IN denotes with
T4025 10410-10413 NN denotes LPS
T4026 10414-10416 CC denotes or
T4027 10417-10421 NN denotes poly
T4028 10422-10423 NN denotes I
T4029 10423-10424 SYM denotes :
T4030 10424-10425 NN denotes C
T4031 10426-10434 VB denotes resulted
T4032 10435-10437 IN denotes in
T4033 10438-10447 NN denotes secretion
T4034 10448-10450 IN denotes of
T4035 10451-10454 NN denotes IL6
T4036 10454-10455 -COMMA- denotes ,
T4037 10456-10463 NN denotes IL12P70
T4038 10463-10464 -COMMA- denotes ,
T4039 10465-10468 CC denotes and
T4040 10469-10474 NN denotes GMCSF
T4041 10475-10479 IN denotes from
T4042 10480-10484 CC denotes both
T4043 10485-10487 NN denotes WT
T4044 10488-10491 CC denotes and
T4045 10492-10496 NN denotes cpdm
T4046 10497-10501 NN denotes BMDC
T4047 10501-10502 -COLON- denotes ;
T4048 10503-10510 RB denotes however
T4049 10510-10511 -COMMA- denotes ,
T4050 10512-10516 NN denotes BMDC
T4051 10517-10521 IN denotes from
T4052 10522-10526 NN denotes cpdm
T4053 10527-10531 NN denotes mice
T4054 10532-10540 VB denotes produced
T4055 10541-10554 RB denotes significantly
T4056 10555-10559 JJ denotes less
T4057 10560-10562 IN denotes of
T4058 10563-10566 DT denotes all
T4059 10567-10572 CD denotes three
T4060 10573-10582 NN denotes cytokines
T4061 10583-10591 VB denotes compared
T4062 10592-10596 IN denotes with
T4063 10597-10599 JJ denotes WT
T4064 10600-10604 NN denotes BMDC
T4065 10605-10606 -LRB- denotes (
T4066 10606-10610 NNP denotes Fig.
T4067 10611-10615 NN denotes 3A–C
T4068 10615-10616 -COMMA- denotes ,
T4069 10617-10620 NN denotes E–F
T4070 10620-10621 -RRB- denotes )
T4071 10623-10626 DT denotes The
T4072 10627-10633 NN denotes amount
T4073 10634-10636 IN denotes of
T4074 10637-10643 JJ denotes nitric
T4075 10644-10649 NN denotes oxide
T4076 10650-10659 VB denotes generated
T4077 10660-10662 IN denotes by
T4078 10663-10669 JJ denotes mutant
T4079 10670-10674 NN denotes BMDC
T4080 10675-10678 VB denotes was
T4081 10679-10683 RB denotes also
T4082 10684-10697 RB denotes significantly
T4083 10698-10705 VB denotes reduced
T4084 10706-10714 VB denotes compared
T4085 10715-10719 IN denotes with
T4086 10720-10722 JJ denotes WT
T4087 10723-10728 NN denotes cells
T4088 10729-10730 -LRB- denotes (
T4089 10730-10734 NN denotes Fig.
T4090 10735-10737 NN denotes 3D
T4091 10737-10738 -RRB- denotes )
T4092 10738-10739 -COMMA- denotes ,
T4093 10740-10750 VB denotes indicating
T4094 10751-10759 RB denotes severely
T4095 10760-10769 VB denotes disrupted
T4096 10770-10780 NN denotes production
T4097 10781-10783 IN denotes of
T4098 10784-10799 JJ denotes proinflammatory
T4099 10800-10809 NN denotes mediators
T4100 10810-10814 IN denotes from
T4101 10815-10832 JJ denotes Sharpin-deficient
T4102 10833-10837 NN denotes BMDC
T4103 10839-10841 FW denotes In
T4104 10842-10846 FW denotes vivo
T4105 10847-10862 NN denotes complementation
T4106 10863-10867 IN denotes with
T4107 10868-10869 DT denotes a
T4108 10870-10877 NNP denotes Sharpin
T4109 10878-10893 JJ denotes gene-containing
T4110 10894-10897 NN denotes BAC
T4111 10898-10906 VB denotes reversed
T4112 10907-10910 DT denotes the
T4113 10911-10920 NN denotes phenotype
T4114 10921-10923 IN denotes of
T4115 10924-10927 DT denotes the
T4116 10928-10934 JJ denotes mutant
T4117 10935-10939 NN denotes mice
T4118 10940-10941 -LRB- denotes [
T4119 10941-10942 CD denotes 2
T4120 10942-10943 -RRB- denotes ]
T4121 10943-10944 -COMMA- denotes ,
T4122 10945-10948 CC denotes and
T4123 10949-10953 NN denotes BMDC
T4124 10954-10963 VB denotes generated
T4125 10964-10968 IN denotes from
T4126 10969-10974 DT denotes these
T4127 10975-10982 VB denotes rescued
T4128 10983-10987 NN denotes mice
T4129 10988-10996 VB denotes secreted
T4130 10997-11010 RB denotes significantly
T4131 11011-11015 JJ denotes more
T4132 11016-11023 NN denotes IL12P70
T4133 11024-11028 IN denotes than
T4134 11029-11033 NN denotes BMDC
T4135 11034-11038 IN denotes from
T4136 11039-11043 NN denotes cpdm
T4137 11044-11048 NN denotes mice
T4138 11049-11050 -LRB- denotes (
T4139 11050-11054 NNP denotes Fig.
T4140 11055-11057 NN denotes 3G
T4141 11057-11058 -RRB- denotes )
T4142 11058-11059 -COMMA- denotes ,
T4143 11060-11070 VB denotes supporting
T4144 11071-11072 DT denotes a
T4145 11073-11082 JJ denotes necessary
T4146 11083-11087 NN denotes role
T4147 11088-11090 IN denotes of
T4148 11091-11098 NN denotes SHARPIN
T4149 11099-11102 IN denotes for
T4150 11103-11106 DT denotes the
T4151 11107-11117 NN denotes production
T4152 11118-11120 IN denotes of
T4153 11121-11128 NN denotes IL12P70
T4154 11130-11132 IN denotes In
T4155 11133-11141 NN denotes addition
T4156 11141-11142 -COMMA- denotes ,
T4157 11143-11146 DT denotes the
T4158 11147-11157 NN denotes transcript
T4159 11158-11164 NN denotes levels
T4160 11165-11167 IN denotes of
T4161 11168-11171 DT denotes the
T4162 11172-11184 JJ denotes inflammatory
T4163 11185-11194 NN denotes cytokines
T4164 11195-11198 NN denotes Il6
T4165 11198-11199 -COMMA- denotes ,
T4166 11200-11207 NN denotes Il12p40
T4167 11207-11208 -COMMA- denotes ,
T4168 11209-11214 NN denotes Gmcsf
T4169 11214-11215 -COMMA- denotes ,
T4170 11216-11219 CC denotes and
T4171 11220-11224 NN denotes Ifnb
T4172 11225-11229 VB denotes were
T4173 11230-11238 VB denotes examined
T4174 11238-11239 -COMMA- denotes ,
T4175 11240-11243 CC denotes and
T4176 11244-11249 DT denotes these
T4177 11250-11254 VB denotes were
T4178 11255-11258 DT denotes all
T4179 11259-11272 RB denotes significantly
T4180 11273-11280 VB denotes reduced
T4181 11281-11283 IN denotes in
T4182 11284-11294 VB denotes stimulated
T4183 11295-11299 NN denotes cpdm
T4184 11300-11304 NN denotes BMDC
T4185 11305-11309 WRB denotes when
T4186 11310-11318 VB denotes compared
T4187 11319-11323 IN denotes with
T4188 11324-11326 JJ denotes WT
T4189 11327-11335 NN denotes controls
T4190 11336-11337 -LRB- denotes (
T4191 11337-11341 NN denotes Fig.
T4192 11342-11343 CD denotes 4
T4193 11343-11344 -RRB- denotes )
T4912 12842-12850 JJ denotes Impaired
T4913 12851-12859 NN denotes cytokine
T4914 12860-12870 NN denotes production
T4915 12871-12873 IN denotes by
T4916 12874-12878 NN denotes cpdm
T4917 12879-12883 NN denotes BMDC
T4918 12884-12886 VB denotes is
T4919 12887-12897 VB denotes correlated
T4920 12898-12902 IN denotes with
T4921 12903-12912 JJ denotes selective
T4922 12913-12920 NN denotes defects
T4923 12921-12923 IN denotes in
T4924 12924-12929 NN denotes NF-κB
T4925 12930-12939 NN denotes signaling
T4926 12940-12945 EX denotes There
T4927 12946-12949 VB denotes are
T4928 12950-12951 DT denotes a
T4929 12952-12958 NN denotes number
T4930 12959-12961 IN denotes of
T4931 12962-12970 JJ denotes possible
T4932 12971-12983 NN denotes explanations
T4933 12984-12987 IN denotes for
T4934 12988-12991 DT denotes the
T4935 12992-13001 JJ denotes defective
T4936 13002-13010 NN denotes cytokine
T4937 13011-13020 NN denotes secretion
T4938 13021-13023 IN denotes in
T4939 13024-13034 VB denotes stimulated
T4940 13035-13039 NN denotes cpdm
T4941 13040-13044 NN denotes BMDC
T4942 13044-13045 -COMMA- denotes ,
T4943 13046-13055 VB denotes including
T4944 13056-13057 CD denotes 1
T4945 13057-13058 -RRB- denotes )
T4946 13059-13066 VB denotes reduced
T4947 13067-13074 NN denotes surface
T4948 13075-13085 NN denotes expression
T4949 13086-13088 IN denotes of
T4950 13089-13092 DT denotes the
T4951 13093-13096 NN denotes LPS
T4952 13097-13105 NN denotes receptor
T4953 13106-13113 NN denotes complex
T4954 13113-13114 -COMMA- denotes ,
T4955 13115-13116 CD denotes 2
T4956 13116-13117 -RRB- denotes )
T4957 13118-13127 VB denotes increased
T4958 13128-13138 NN denotes production
T4959 13139-13141 IN denotes of
T4960 13142-13159 JJ denotes anti-inflammatory
T4961 13160-13169 NN denotes mediators
T4962 13169-13170 -COMMA- denotes ,
T4963 13171-13172 CD denotes 3
T4964 13172-13173 -RRB- denotes )
T4965 13174-13183 VB denotes increased
T4966 13184-13194 NN denotes expression
T4967 13195-13197 IN denotes of
T4968 13198-13206 JJ denotes negative
T4969 13207-13217 NN denotes regulators
T4970 13218-13220 IN denotes of
T4971 13221-13224 NN denotes TLR
T4972 13225-13233 NN denotes pathways
T4973 13233-13234 -COMMA- denotes ,
T4974 13235-13238 CC denotes and
T4975 13239-13240 CD denotes 4
T4976 13240-13241 -RRB- denotes )
T4977 13242-13250 JJ denotes impaired
T4978 13251-13262 JJ denotes TLR-induced
T4979 13263-13272 NN denotes signaling
T4980 13273-13283 NN denotes activation
T4981 13285-13287 PRP denotes We
T4982 13288-13298 VB denotes determined
T4983 13299-13302 DT denotes the
T4984 13303-13310 NN denotes surface
T4985 13311-13321 NN denotes expression
T4986 13322-13324 IN denotes of
T4987 13325-13328 DT denotes the
T4988 13329-13332 NN denotes LPS
T4989 13333-13341 NN denotes receptor
T4990 13342-13349 NN denotes complex
T4991 13350-13354 WDT denotes that
T4992 13355-13364 VB denotes comprises
T4993 13365-13369 NN denotes TLR4
T4994 13369-13370 -COMMA- denotes ,
T4995 13371-13374 DT denotes the
T4996 13375-13384 JJ denotes accessory
T4997 13385-13393 NN denotes proteins
T4998 13394-13398 NN denotes CD14
T4999 13399-13402 CC denotes and
T5000 13403-13410 JJ denotes myeloid
T5001 13411-13426 NN denotes differentiation
T5002 13427-13433 NN denotes factor
T5003 13434-13435 CD denotes 2
T5004 13436-13437 -LRB- denotes (
T5005 13437-13445 NN denotes MD2/LY96
T5006 13445-13446 -RRB- denotes )
T5007 13447-13448 -LRB- denotes [
T5008 13448-13450 CD denotes 19
T5009 13450-13451 -RRB- denotes ]
T5010 13453-13457 NN denotes Flow
T5011 13458-13468 JJ denotes cytometric
T5012 13469-13477 NN denotes analysis
T5013 13478-13483 VB denotes shows
T5014 13484-13488 IN denotes that
T5015 13489-13492 DT denotes the
T5016 13493-13503 NN denotes expression
T5017 13504-13510 NN denotes levels
T5018 13511-13513 IN denotes of
T5019 13514-13518 NN denotes CD14
T5020 13519-13522 CC denotes and
T5021 13523-13531 NN denotes TLR4/MD2
T5022 13532-13539 IN denotes between
T5023 13540-13542 NN denotes WT
T5024 13543-13546 CC denotes and
T5025 13547-13551 NN denotes cpdm
T5026 13552-13556 NN denotes BMDC
T5027 13557-13561 VB denotes were
T5028 13562-13569 JJ denotes similar
T5029 13570-13571 -LRB- denotes (
T5030 13571-13575 NN denotes Fig.
T5031 13576-13578 NN denotes 5A
T5032 13578-13579 -RRB- denotes )
T5033 13581-13583 PRP denotes We
T5034 13584-13588 RB denotes then
T5035 13589-13599 VB denotes quantified
T5036 13600-13603 DT denotes the
T5037 13604-13613 NN denotes secretion
T5038 13614-13616 IN denotes of
T5039 13617-13620 DT denotes the
T5040 13621-13632 JJ denotes suppressive
T5041 13633-13642 NN denotes cytokines
T5042 13643-13647 NN denotes IL10
T5043 13648-13652 WDT denotes that
T5044 13653-13656 MD denotes can
T5045 13657-13664 VB denotes inhibit
T5046 13665-13669 NN denotes IL12
T5047 13670-13679 NN denotes secretion
T5048 13680-13682 IN denotes in
T5049 13683-13685 DT denotes an
T5050 13686-13695 JJ denotes autocrine
T5051 13696-13702 NN denotes manner
T5052 13703-13704 -LRB- denotes [
T5053 13704-13706 CD denotes 20
T5054 13706-13707 -RRB- denotes ]
T5055 13707-13708 -COMMA- denotes ,
T5056 13709-13710 -LRB- denotes [
T5057 13710-13712 CD denotes 21
T5058 13712-13713 -RRB- denotes ]
T5059 13715-13718 DT denotes The
T5060 13719-13731 NN denotes supernatants
T5061 13732-13736 IN denotes from
T5062 13737-13751 JJ denotes LPS-stimulated
T5063 13752-13756 NN denotes cpdm
T5064 13757-13761 NN denotes BMDC
T5065 13762-13771 VB denotes contained
T5066 13772-13785 RB denotes significantly
T5067 13786-13791 JJ denotes lower
T5068 13792-13798 NN denotes levels
T5069 13799-13801 IN denotes of
T5070 13802-13806 NN denotes IL10
T5071 13807-13811 IN denotes than
T5072 13812-13822 VB denotes stimulated
T5073 13823-13825 NN denotes WT
T5074 13826-13830 NN denotes BMDC
T5075 13831-13832 -LRB- denotes (
T5076 13832-13836 NN denotes Fig.
T5077 13837-13839 NN denotes 5B
T5078 13839-13840 -RRB- denotes )
T5079 13840-13841 -COMMA- denotes ,
T5080 13842-13852 VB denotes suggesting
T5081 13853-13857 IN denotes that
T5082 13858-13862 NN denotes IL10
T5083 13863-13866 VB denotes was
T5084 13867-13870 RB denotes not
T5085 13871-13882 JJ denotes responsible
T5086 13883-13886 IN denotes for
T5087 13887-13896 VB denotes decreased
T5088 13897-13906 NN denotes secretion
T5089 13907-13909 IN denotes of
T5090 13910-13917 NN denotes IL12P70
T5091 13918-13920 IN denotes by
T5092 13921-13925 NN denotes cpdm
T5093 13926-13930 NN denotes BMDC
T5094 13932-13941 VB denotes Increased
T5095 13942-13952 NN denotes expression
T5096 13953-13955 IN denotes of
T5097 13956-13957 DT denotes a
T5098 13958-13966 JJ denotes negative
T5099 13967-13976 NN denotes regulator
T5100 13977-13979 IN denotes of
T5101 13980-13983 NN denotes TLR
T5102 13984-13993 NN denotes signaling
T5103 13994-13998 JJ denotes such
T5104 13999-14001 IN denotes as
T5105 14002-14005 NN denotes A20
T5106 14006-14007 -LRB- denotes [
T5107 14007-14009 CD denotes 22
T5108 14009-14010 -RRB- denotes ]
T5109 14011-14014 MD denotes may
T5110 14015-14019 RB denotes also
T5111 14020-14028 VB denotes suppress
T5112 14029-14037 NN denotes cytokine
T5113 14038-14047 NN denotes secretion
T5114 14049-14056 RB denotes However
T5115 14056-14057 -COMMA- denotes ,
T5116 14058-14061 DT denotes the
T5117 14062-14072 NN denotes transcript
T5118 14073-14078 NN denotes level
T5119 14079-14081 IN denotes of
T5120 14082-14085 NN denotes A20
T5121 14086-14089 VB denotes was
T5122 14090-14095 JJ denotes lower
T5123 14096-14098 IN denotes in
T5124 14099-14112 JJ denotes LPS-activated
T5125 14113-14117 NN denotes cpdm
T5126 14118-14122 NN denotes BMDC
T5127 14123-14127 IN denotes than
T5128 14128-14130 JJ denotes WT
T5129 14131-14139 NN denotes controls
T5130 14140-14141 -LRB- denotes (
T5131 14141-14145 NNP denotes Fig.
T5132 14146-14148 NN denotes 5C
T5133 14148-14149 -RRB- denotes )
T5134 14149-14150 -COMMA- denotes ,
T5135 14151-14158 RB denotes thereby
T5136 14159-14165 VB denotes ruling
T5137 14166-14169 RP denotes out
T5138 14170-14184 NN denotes overexpression
T5139 14185-14187 IN denotes of
T5140 14188-14191 NN denotes A20
T5141 14192-14194 IN denotes as
T5142 14195-14196 DT denotes a
T5143 14197-14203 NN denotes factor
T5144 14204-14206 IN denotes in
T5145 14207-14210 DT denotes the
T5146 14211-14218 VB denotes reduced
T5147 14219-14227 NN denotes cytokine
T5148 14228-14238 NN denotes production
T5149 15014-15017 DT denotes The
T5150 15018-15031 NN denotes transcription
T5151 15032-15034 IN denotes of
T5152 15035-15049 JJ denotes TLR3/4-induced
T5153 15050-15065 JJ denotes proinflammatory
T5154 15066-15079 NN denotes intermediates
T5155 15080-15082 VB denotes is
T5156 15083-15090 RB denotes tightly
T5157 15091-15100 VB denotes regulated
T5158 15101-15103 IN denotes by
T5159 15104-15112 JJ denotes cellular
T5160 15113-15122 NN denotes signaling
T5161 15123-15131 NN denotes pathways
T5162 15131-15132 -COMMA- denotes ,
T5163 15133-15135 IN denotes in
T5164 15136-15146 JJ denotes particular
T5165 15147-15152 NN denotes NF-κB
T5166 15152-15153 -COMMA- denotes ,
T5167 15154-15163 NN denotes TBK1/IRF3
T5168 15163-15164 -COMMA- denotes ,
T5169 15165-15168 CC denotes and
T5170 15169-15173 NN denotes MAPK
T5171 15174-15175 -LRB- denotes [
T5172 15175-15177 CD denotes 23
T5173 15177-15178 -RRB- denotes ]
T5174 15178-15179 NNP denotes –
T5175 15179-15180 -LRB- denotes [
T5176 15180-15182 CD denotes 27
T5177 15182-15183 -RRB- denotes ]
T5178 15185-15187 PRP denotes We
T5179 15188-15192 RB denotes next
T5180 15193-15203 VB denotes determined
T5181 15204-15206 IN denotes if
T5182 15207-15216 VB denotes disrupted
T5183 15217-15222 NN denotes NF-κB
T5184 15222-15223 -COMMA- denotes ,
T5185 15224-15233 NN denotes TBK1/IRF3
T5186 15233-15234 -COMMA- denotes ,
T5187 15235-15241 CC denotes and/or
T5188 15242-15246 NN denotes MAPK
T5189 15247-15256 NN denotes signaling
T5190 15257-15260 MD denotes may
T5191 15261-15269 VB denotes underlie
T5192 15270-15273 DT denotes the
T5193 15274-15282 JJ denotes impaired
T5194 15283-15291 NN denotes cytokine
T5195 15292-15302 NN denotes production
T5196 15303-15307 IN denotes from
T5197 15308-15318 VB denotes stimulated
T5198 15319-15336 JJ denotes Sharpin-deficient
T5199 15337-15341 NN denotes BMDC
T5200 15343-15359 JJ denotes Stimulus-induced
T5201 15360-15375 NN denotes phosphorylation
T5202 15376-15378 IN denotes of
T5203 15379-15382 DT denotes the
T5204 15383-15386 NN denotes IκB
T5205 15387-15393 NN denotes kinase
T5206 15394-15395 -LRB- denotes (
T5207 15395-15401 NN denotes IKK1/2
T5208 15401-15402 -RRB- denotes )
T5209 15403-15405 VB denotes is
T5210 15406-15408 DT denotes an
T5211 15409-15418 JJ denotes essential
T5212 15419-15423 NN denotes step
T5213 15424-15426 IN denotes in
T5214 15427-15432 NN denotes NF-κB
T5215 15433-15442 NN denotes signaling
T5216 15442-15443 -COMMA- denotes ,
T5217 15444-15452 VB denotes allowing
T5218 15453-15468 NN denotes phosphorylation
T5219 15469-15472 CC denotes and
T5220 15473-15492 JJ denotes proteasome-mediated
T5221 15493-15504 NN denotes degradation
T5222 15505-15507 IN denotes of
T5223 15508-15511 DT denotes the
T5224 15512-15517 NN denotes NF-κB
T5225 15518-15527 NN denotes inhibitor
T5226 15528-15532 NN denotes IκBα
T5227 15533-15535 TO denotes to
T5228 15536-15543 VB denotes release
T5229 15544-15547 DT denotes the
T5230 15548-15553 NN denotes NF-κB
T5231 15554-15567 NN denotes transcription
T5232 15568-15575 NN denotes factors
T5233 15576-15580 IN denotes into
T5234 15581-15584 DT denotes the
T5235 15585-15592 NN denotes nucleus
T5236 15594-15597 DT denotes The
T5237 15598-15604 NN denotes amount
T5238 15605-15607 IN denotes of
T5239 15608-15622 VB denotes phosphorylated
T5240 15623-15629 NN denotes IKK1/2
T5241 15630-15631 -LRB- denotes (
T5242 15631-15639 NN denotes p-IKK1/2
T5243 15639-15640 -RRB- denotes )
T5244 15641-15644 CC denotes and
T5245 15645-15649 NN denotes IκBα
T5246 15650-15651 -LRB- denotes (
T5247 15651-15657 NN denotes p-IκBα
T5248 15657-15658 -RRB- denotes )
T5249 15659-15668 VB denotes following
T5250 15669-15679 NN denotes incubation
T5251 15680-15684 IN denotes with
T5252 15685-15688 NN denotes LPS
T5253 15689-15691 CC denotes or
T5254 15692-15696 NN denotes poly
T5255 15697-15698 NN denotes I
T5256 15698-15699 SYM denotes :
T5257 15699-15700 NN denotes C
T5258 15701-15704 VB denotes was
T5259 15705-15713 RB denotes severely
T5260 15714-15723 VB denotes decreased
T5261 15724-15726 IN denotes in
T5262 15727-15731 NN denotes cpdm
T5263 15732-15736 NN denotes BMDC
T5264 15737-15739 IN denotes as
T5265 15740-15748 VB denotes compared
T5266 15749-15753 IN denotes with
T5267 15754-15756 JJ denotes WT
T5268 15757-15765 NN denotes controls
T5269 15766-15767 -LRB- denotes (
T5270 15767-15771 NN denotes Fig.
T5271 15772-15773 CD denotes 6
T5272 15773-15774 -RRB- denotes )
T5273 15776-15779 DT denotes The
T5274 15780-15784 NN denotes cpdm
T5275 15785-15789 NN denotes BMDC
T5276 15790-15799 VB denotes exhibited
T5277 15800-15807 JJ denotes similar
T5278 15808-15814 NN denotes levels
T5279 15815-15817 IN denotes of
T5280 15818-15822 NN denotes TBK1
T5281 15822-15823 -COMMA- denotes ,
T5282 15824-15830 NN denotes ERK1/2
T5283 15830-15831 -COMMA- denotes ,
T5284 15832-15835 CC denotes and
T5285 15836-15839 NN denotes p38
T5286 15840-15855 NN denotes phosphorylation
T5287 15856-15858 TO denotes to
T5288 15859-15864 DT denotes those
T5289 15865-15867 IN denotes of
T5290 15868-15870 JJ denotes WT
T5291 15871-15876 NN denotes cells
T5292 15877-15878 -LRB- denotes (
T5293 15878-15882 NN denotes Fig.
T5294 15883-15884 CD denotes 6
T5295 15884-15885 -RRB- denotes )
T5296 15887-15892 DT denotes These
T5297 15893-15900 NN denotes results
T5298 15901-15909 VB denotes indicate
T5299 15910-15914 IN denotes that
T5300 15915-15918 DT denotes the
T5301 15919-15926 NN denotes absence
T5302 15927-15929 IN denotes of
T5303 15930-15940 JJ denotes functional
T5304 15941-15948 NN denotes SHARPIN
T5305 15949-15958 VB denotes decreased
T5306 15959-15964 NN denotes NF-κB
T5307 15965-15975 NN denotes activation
T5308 15976-15979 CC denotes but
T5309 15980-15983 VB denotes did
T5310 15984-15987 RB denotes not
T5311 15988-15994 VB denotes affect
T5312 15995-16004 NN denotes TBK1/IRF3
T5313 16004-16005 -COMMA- denotes ,
T5314 16006-16012 NN denotes ERK1/2
T5315 16012-16013 -COMMA- denotes ,
T5316 16014-16017 CC denotes and
T5317 16018-16021 NN denotes p38
T5318 16022-16031 NN denotes signaling
T5319 16032-16034 IN denotes in
T5320 16035-16039 NN denotes BMDC
T6137 16693-16703 JJ denotes Th2-biased
T6138 16704-16718 NN denotes immunogenicity
T6139 16719-16721 IN denotes of
T6140 16722-16732 VB denotes stimulated
T6141 16733-16737 NN denotes cpdm
T6142 16738-16742 NN denotes BMDC
T6143 16743-16746 DT denotes The
T6144 16747-16756 JJ denotes defective
T6145 16757-16761 NN denotes IL12
T6146 16762-16772 NN denotes production
T6147 16773-16774 -LRB- denotes (
T6148 16774-16778 NN denotes Fig.
T6149 16779-16781 NN denotes 3A
T6150 16781-16782 -RRB- denotes )
T6151 16783-16786 CC denotes and
T6152 16787-16799 JJ denotes Th2-dominant
T6153 16800-16808 NN denotes cytokine
T6154 16809-16816 NN denotes profile
T6155 16817-16819 IN denotes in
T6156 16820-16824 NN denotes cpdm
T6157 16825-16829 NN denotes mice
T6158 16830-16831 -LRB- denotes [
T6159 16831-16832 CD denotes 5
T6160 16832-16833 -RRB- denotes ]
T6161 16834-16841 VB denotes suggest
T6162 16842-16846 IN denotes that
T6163 16847-16850 DT denotes the
T6164 16851-16858 NN denotes absence
T6165 16859-16861 IN denotes of
T6166 16862-16869 NN denotes SHARPIN
T6167 16870-16877 VB denotes affects
T6168 16878-16881 DT denotes the
T6169 16882-16889 NN denotes ability
T6170 16890-16892 IN denotes of
T6171 16893-16897 NN denotes cpdm
T6172 16898-16902 NN denotes BMDC
T6173 16903-16905 TO denotes to
T6174 16906-16912 VB denotes induce
T6175 16913-16914 NN denotes T
T6176 16915-16919 NN denotes cell
T6177 16920-16935 NN denotes differentiation
T6178 16936-16940 IN denotes into
T6179 16941-16949 NN denotes effector
T6180 16950-16955 NN denotes cells
T6181 16957-16967 NN denotes Co-culture
T6182 16968-16970 IN denotes of
T6183 16971-16981 JJ denotes allogeneic
T6184 16982-16987 NN denotes naïve
T6185 16988-16992 JJ denotes CD4+
T6186 16993-16994 NN denotes T
T6187 16995-17000 NN denotes cells
T6188 17001-17005 IN denotes with
T6189 17006-17008 NN denotes WT
T6190 17009-17013 NN denotes BMDC
T6191 17014-17024 VB denotes stimulated
T6192 17025-17029 IN denotes with
T6193 17030-17033 NN denotes LPS
T6194 17034-17036 CC denotes or
T6195 17037-17041 NN denotes poly
T6196 17042-17043 NN denotes I
T6197 17043-17044 SYM denotes :
T6198 17044-17045 NN denotes C
T6199 17046-17054 VB denotes elicited
T6200 17055-17061 JJ denotes robust
T6201 17062-17066 NN denotes IFNγ
T6202 17067-17077 NN denotes production
T6203 17077-17078 -COMMA- denotes ,
T6204 17079-17086 IN denotes whereas
T6205 17087-17090 DT denotes the
T6206 17091-17104 NN denotes concentration
T6207 17105-17107 IN denotes of
T6208 17108-17112 NN denotes IFNγ
T6209 17113-17115 IN denotes in
T6210 17116-17120 NN denotes cpdm
T6211 17121-17127 NN denotes BMDC-T
T6212 17128-17132 NN denotes cell
T6213 17133-17141 NN denotes cultures
T6214 17142-17145 VB denotes was
T6215 17146-17159 RB denotes significantly
T6216 17160-17165 JJ denotes lower
T6217 17166-17171 IN denotes after
T6218 17172-17175 NN denotes LPS
T6219 17176-17187 NN denotes stimulation
T6220 17188-17189 -LRB- denotes (
T6221 17189-17193 NNP denotes Fig.
T6222 17194-17196 NN denotes 7A
T6223 17196-17197 -RRB- denotes )
T6224 17197-17198 -COMMA- denotes ,
T6225 17199-17209 VB denotes indicating
T6226 17210-17218 JJ denotes impaired
T6227 17219-17233 JJ denotes Th1-polarizing
T6228 17234-17243 NN denotes abilities
T6229 17244-17246 IN denotes of
T6230 17247-17251 NN denotes cpdm
T6231 17252-17256 NN denotes BMDC
T6232 17258-17260 IN denotes In
T6233 17261-17269 NN denotes addition
T6234 17270-17272 TO denotes to
T6235 17273-17279 NN denotes TLR3/4
T6236 17280-17288 NN denotes agonists
T6237 17288-17289 -COMMA- denotes ,
T6238 17290-17293 DT denotes the
T6239 17294-17298 NN denotes TLR2
T6240 17299-17305 NN denotes ligand
T6241 17306-17313 NN denotes Pam3CYS
T6242 17314-17317 VB denotes was
T6243 17318-17322 VB denotes used
T6244 17323-17328 IN denotes since
T6245 17329-17331 PRP denotes it
T6246 17332-17335 VB denotes has
T6247 17336-17340 VB denotes been
T6248 17341-17346 VB denotes shown
T6249 17347-17349 TO denotes to
T6250 17350-17356 VB denotes induce
T6251 17357-17361 CC denotes both
T6252 17362-17365 NN denotes Th1
T6253 17366-17369 CC denotes and
T6254 17370-17373 NN denotes Th2
T6255 17374-17383 NN denotes responses
T6256 17384-17385 -LRB- denotes [
T6257 17385-17387 CD denotes 28
T6258 17387-17388 -RRB- denotes ]
T6259 17388-17389 CD denotes –
T6260 17389-17390 -LRB- denotes [
T6261 17390-17392 CD denotes 30
T6262 17392-17393 -RRB- denotes ]
T6263 17395-17410 JJ denotes Pam3CYS-matured
T6264 17411-17413 NN denotes WT
T6265 17414-17418 NN denotes BMDC
T6266 17419-17426 VB denotes induced
T6267 17427-17433 JJ denotes robust
T6268 17434-17438 NN denotes IFNγ
T6269 17439-17449 NN denotes production
T6270 17450-17452 IN denotes at
T6271 17453-17454 DT denotes a
T6272 17455-17468 RB denotes significantly
T6273 17469-17475 JJ denotes higher
T6274 17476-17481 NN denotes level
T6275 17482-17486 IN denotes than
T6276 17487-17491 NN denotes cpdm
T6277 17492-17496 NN denotes BMDC
T6278 17497-17498 -LRB- denotes (
T6279 17498-17502 NNP denotes Fig.
T6280 17503-17505 NN denotes 7A
T6281 17505-17506 -RRB- denotes )
T6282 17508-17511 DT denotes The
T6283 17512-17519 VB denotes reduced
T6284 17520-17523 NN denotes Th1
T6285 17524-17539 NN denotes differentiation
T6286 17540-17549 VB denotes following
T6287 17550-17557 NN denotes Pam3CYS
T6288 17558-17569 NN denotes stimulation
T6289 17570-17572 VB denotes is
T6290 17573-17583 JJ denotes consistent
T6291 17584-17588 IN denotes with
T6292 17589-17592 DT denotes the
T6293 17593-17599 JJ denotes recent
T6294 17600-17606 NN denotes report
T6295 17607-17609 IN denotes of
T6296 17610-17619 VB denotes decreased
T6297 17620-17624 NN denotes IL12
T6298 17625-17635 NN denotes production
T6299 17636-17638 IN denotes in
T6300 17639-17643 NN denotes cpdm
T6301 17644-17655 NN denotes macrophages
T6302 17656-17665 VB denotes following
T6303 17666-17670 NN denotes TLR2
T6304 17671-17682 NN denotes stimulation
T6305 17683-17684 -LRB- denotes [
T6306 17684-17686 CD denotes 31
T6307 17686-17687 -RRB- denotes ]
T6308 17689-17691 IN denotes In
T6309 17692-17700 NN denotes contrast
T6310 17700-17701 -COMMA- denotes ,
T6311 17702-17706 RB denotes more
T6312 17707-17719 JJ denotes Th2-specific
T6313 17720-17723 NN denotes IL4
T6314 17724-17732 NN denotes cytokine
T6315 17733-17736 VB denotes was
T6316 17737-17745 VB denotes produced
T6317 17746-17748 IN denotes in
T6318 17749-17752 DT denotes the
T6319 17753-17757 NN denotes cpdm
T6320 17758-17762 NN denotes BMDC
T6321 17763-17774 NN denotes co-cultures
T6322 17775-17779 IN denotes than
T6323 17780-17783 DT denotes the
T6324 17784-17786 NN denotes WT
T6325 17787-17794 NN denotes control
T6326 17795-17796 -LRB- denotes (
T6327 17796-17800 NNP denotes Fig.
T6328 17801-17803 NN denotes 7B
T6329 17803-17804 -RRB- denotes )
T6330 17804-17805 -COMMA- denotes ,
T6331 17806-17816 VB denotes suggesting
T6332 17817-17827 JJ denotes Th2-skewed
T6333 17828-17842 NN denotes immunogenicity
T6334 17843-17845 IN denotes of
T6335 17846-17850 NN denotes cpdm
T6336 17851-17855 NN denotes BMDC
T6337 17857-17860 DT denotes The
T6338 17861-17871 NN denotes production
T6339 17872-17874 IN denotes of
T6340 17875-17888 JJ denotes Th17-specific
T6341 17889-17897 NN denotes cytokine
T6342 17898-17903 NN denotes IL17A
T6343 17904-17913 VB denotes following
T6344 17914-17917 NN denotes LPS
T6345 17918-17929 NN denotes stimulation
T6346 17930-17932 IN denotes of
T6347 17933-17942 JJ denotes dendritic
T6348 17943-17948 NN denotes cells
T6349 17949-17952 VB denotes was
T6350 17953-17960 JJ denotes similar
T6351 17961-17968 IN denotes between
T6352 17969-17979 VB denotes stimulated
T6353 17980-17982 NN denotes WT
T6354 17983-17986 CC denotes and
T6355 17987-17991 NN denotes cpdm
T6356 17992-17996 NN denotes BMDC
T6357 17997-18007 NN denotes cocultures
T6358 18008-18009 -LRB- denotes (
T6359 18009-18013 NN denotes data
T6360 18014-18017 RB denotes not
T6361 18018-18023 VB denotes shown
T6362 18023-18024 -RRB- denotes )
T6363 18026-18033 IN denotes Despite
T6364 18034-18037 DT denotes the
T6365 18038-18046 JJ denotes distinct
T6366 18047-18051 NN denotes Th1-
T6367 18052-18055 CC denotes and
T6368 18056-18071 JJ denotes Th2-stimulating
T6369 18072-18081 NN denotes abilities
T6370 18082-18084 IN denotes of
T6371 18085-18087 NN denotes WT
T6372 18088-18091 CC denotes and
T6373 18092-18096 NN denotes cpdm
T6374 18097-18101 NN denotes BMDC
T6375 18101-18102 -COMMA- denotes ,
T6376 18103-18107 PRP denotes they
T6377 18108-18112 VB denotes were
T6378 18113-18120 RB denotes equally
T6379 18121-18130 JJ denotes effective
T6380 18131-18133 IN denotes in
T6381 18134-18137 NN denotes IL2
T6382 18138-18148 NN denotes production
T6383 18149-18153 IN denotes from
T6384 18154-18160 NN denotes BMDC-T
T6385 18161-18165 NN denotes cell
T6386 18166-18177 NN denotes co-cultures
T6387 18178-18184 IN denotes except
T6388 18185-18188 IN denotes for
T6389 18189-18193 NN denotes poly
T6390 18194-18195 NN denotes I
T6391 18195-18196 -COLON- denotes :
T6392 18196-18197 NN denotes C
T6393 18198-18209 NN denotes stimulation
T6394 18210-18215 WRB denotes where
T6395 18216-18218 NN denotes WT
T6396 18219-18223 NN denotes BMDC
T6397 18224-18231 VB denotes induced
T6398 18232-18236 JJ denotes more
T6399 18237-18240 NN denotes IL2
T6400 18241-18245 IN denotes than
T6401 18246-18250 NN denotes cpdm
T6402 18251-18255 NN denotes BMDC
T6403 18256-18257 -LRB- denotes (
T6404 18257-18261 NNP denotes Fig.
T6405 18262-18264 NN denotes 7C
T6406 18264-18265 -RRB- denotes )
T6407 18267-18270 DT denotes The
T6408 18271-18281 JJ denotes Th2-biased
T6409 18282-18293 VB denotes stimulating
T6410 18294-18304 NN denotes capability
T6411 18305-18307 IN denotes of
T6412 18308-18312 NN denotes cpdm
T6413 18313-18317 NN denotes BMDC
T6414 18318-18322 WRB denotes when
T6415 18323-18334 VB denotes co-cultured
T6416 18335-18339 IN denotes with
T6417 18340-18350 JJ denotes allogeneic
T6418 18351-18356 NN denotes naïve
T6419 18357-18361 JJ denotes CD4+
T6420 18362-18363 NN denotes T
T6421 18364-18369 NN denotes cells
T6422 18370-18372 VB denotes is
T6423 18373-18383 JJ denotes consistent
T6424 18384-18388 IN denotes with
T6425 18389-18392 DT denotes the
T6426 18393-18405 JJ denotes Th2-dominant
T6427 18406-18414 NN denotes cytokine
T6428 18415-18424 NN denotes phenotype
T6429 18425-18433 VB denotes observed
T6430 18434-18436 IN denotes in
T6431 18437-18441 NN denotes cpdm
T6432 18442-18446 NN denotes mice
T7942 19355-19358 DT denotes The
T7943 19359-19366 JJ denotes present
T7944 19367-19373 NN denotes report
T7945 19374-19380 VB denotes showed
T7946 19381-19385 IN denotes that
T7947 19386-19390 JJ denotes null
T7948 19391-19400 NN denotes mutations
T7949 19401-19403 IN denotes of
T7950 19404-19407 DT denotes the
T7951 19408-19413 NN denotes mouse
T7952 19414-19421 NN denotes Sharpin
T7953 19422-19426 NN denotes gene
T7954 19427-19430 VB denotes did
T7955 19431-19434 RB denotes not
T7956 19435-19441 VB denotes affect
T7957 19442-19445 DT denotes the
T7958 19446-19458 JJ denotes steady-state
T7959 19459-19471 NN denotes distribution
T7960 19472-19474 IN denotes of
T7961 19475-19482 JJ denotes splenic
T7962 19483-19485 NN denotes DC
T7963 19486-19493 NN denotes subsets
T7964 19494-19497 CC denotes nor
T7965 19498-19501 DT denotes the
T7966 19502-19513 NN denotes development
T7967 19514-19517 CC denotes and
T7968 19518-19527 NN denotes phenotype
T7969 19528-19530 IN denotes of
T7970 19531-19535 NN denotes BMDC
T7971 19537-19544 RB denotes However
T7972 19544-19545 -COMMA- denotes ,
T7973 19546-19550 NN denotes loss
T7974 19551-19553 IN denotes of
T7975 19554-19561 NN denotes SHARPIN
T7976 19562-19575 RB denotes significantly
T7977 19576-19586 VB denotes diminished
T7978 19587-19590 DT denotes the
T7979 19591-19599 NN denotes capacity
T7980 19600-19602 IN denotes of
T7981 19603-19607 NN denotes BMDC
T7982 19608-19610 TO denotes to
T7983 19611-19618 VB denotes secrete
T7984 19619-19631 JJ denotes inflammatory
T7985 19632-19641 NN denotes cytokines
T7986 19642-19645 CC denotes and
T7987 19646-19652 JJ denotes nitric
T7988 19653-19658 NN denotes oxide
T7989 19660-19663 DT denotes The
T7990 19664-19674 VB denotes attenuated
T7991 19675-19683 NN denotes cytokine
T7992 19684-19694 NN denotes production
T7993 19695-19698 VB denotes was
T7994 19699-19702 RB denotes not
T7995 19703-19706 JJ denotes due
T7996 19707-19709 TO denotes to
T7997 19710-19713 DT denotes the
T7998 19714-19722 NN denotes presence
T7999 19723-19725 IN denotes of
T8000 19726-19743 JJ denotes anti-inflammatory
T8001 19744-19754 NN denotes inhibitors
T8002 19754-19755 -COMMA- denotes ,
T8003 19756-19759 CC denotes and
T8004 19760-19763 MD denotes can
T8005 19764-19766 VB denotes be
T8006 19767-19774 RB denotes largely
T8007 19775-19784 VB denotes explained
T8008 19785-19787 IN denotes by
T8009 19788-19797 JJ denotes selective
T8010 19798-19810 NN denotes inactivation
T8011 19811-19813 IN denotes of
T8012 19814-19819 NN denotes NF-κB
T8013 19820-19829 NN denotes signaling
T8014 19831-19841 VB denotes Stimulated
T8015 19842-19846 NN denotes cpdm
T8016 19847-19851 NN denotes BMDC
T8017 19852-19861 VB denotes exhibited
T8018 19862-19872 JJ denotes Th2-biased
T8019 19873-19874 NN denotes T
T8020 19875-19890 JJ denotes cell-polarizing
T8021 19891-19903 NN denotes capabilities
T8022 19903-19904 -COMMA- denotes ,
T8023 19905-19915 JJ denotes consistent
T8024 19916-19920 IN denotes with
T8025 19921-19924 DT denotes the
T8026 19925-19928 NN denotes Th2
T8027 19929-19946 JJ denotes cytokine-dominant
T8028 19947-19956 NN denotes phenotype
T8029 19957-19959 IN denotes in
T8030 19960-19964 NN denotes cpdm
T8031 19965-19969 NN denotes mice
T8032 19971-19979 RB denotes Together
T8033 19979-19980 -COMMA- denotes ,
T8034 19981-19986 DT denotes these
T8035 19987-19994 NN denotes results
T8036 19995-20003 VB denotes indicate
T8037 20004-20006 DT denotes an
T8038 20007-20020 JJ denotes indispensible
T8039 20021-20025 NN denotes role
T8040 20026-20028 IN denotes of
T8041 20029-20036 NN denotes SHARPIN
T8042 20037-20039 IN denotes in
T8043 20040-20050 VB denotes regulating
T8044 20051-20053 NN denotes DC
T8045 20054-20067 JJ denotes immunological
T8046 20068-20077 NN denotes functions
T8047 20077-20078 -COMMA- denotes ,
T8048 20079-20089 NN denotes disruption
T8049 20090-20092 IN denotes of
T8050 20093-20098 WDT denotes which
T8051 20099-20102 MD denotes may
T8052 20103-20113 VB denotes contribute
T8053 20114-20116 TO denotes to
T8054 20117-20120 DT denotes the
T8055 20121-20132 NN denotes development
T8056 20133-20135 IN denotes of
T8057 20136-20142 JJ denotes immune
T8058 20143-20151 NN denotes diseases
T8059 20153-20158 IN denotes Since
T8060 20159-20161 NN denotes WT
T8061 20162-20165 CC denotes and
T8062 20166-20170 NN denotes cpdm
T8063 20171-20175 NN denotes mice
T8064 20176-20179 VB denotes are
T8065 20180-20184 DT denotes both
T8066 20185-20193 JJ denotes specific
T8067 20194-20207 NN denotes pathogen-free
T8068 20207-20208 -COMMA- denotes ,
T8069 20209-20212 DT denotes the
T8070 20213-20219 NN denotes nature
T8071 20220-20222 IN denotes of
T8072 20223-20226 DT denotes the
T8073 20227-20234 NN denotes trigger
T8074 20235-20237 IN denotes of
T8075 20238-20241 DT denotes the
T8076 20242-20248 JJ denotes severe
T8077 20249-20261 NN denotes inflammation
T8078 20262-20264 IN denotes in
T8079 20265-20269 NN denotes cpdm
T8080 20270-20274 NN denotes mice
T8081 20275-20277 VB denotes is
T8082 20278-20281 RB denotes not
T8083 20282-20289 JJ denotes obvious
T8084 20291-20294 CD denotes One
T8085 20295-20299 JJ denotes such
T8086 20300-20310 VB denotes initiating
T8087 20311-20317 NN denotes factor
T8088 20318-20323 MD denotes could
T8089 20324-20326 VB denotes be
T8090 20327-20337 JJ denotes endogenous
T8091 20338-20347 JJ denotes apoptotic
T8092 20348-20354 CC denotes and/or
T8093 20355-20363 JJ denotes necrotic
T8094 20364-20369 NN denotes cells
T8095 20370-20374 WDT denotes that
T8096 20375-20378 MD denotes can
T8097 20379-20386 VB denotes release
T8098 20387-20404 JJ denotes danger-associated
T8099 20405-20414 JJ denotes molecular
T8100 20415-20423 NN denotes patterns
T8101 20424-20425 -LRB- denotes (
T8102 20425-20430 NN denotes DAMPs
T8103 20430-20431 -RRB- denotes )
T8104 20432-20434 TO denotes to
T8105 20435-20441 VB denotes launch
T8106 20442-20445 CC denotes and
T8107 20446-20453 VB denotes amplify
T8108 20454-20456 DT denotes an
T8109 20457-20469 JJ denotes inflammatory
T8110 20470-20478 NN denotes response
T8111 20479-20480 -LRB- denotes [
T8112 20480-20482 CD denotes 32
T8113 20482-20483 -RRB- denotes ]
T8114 20485-20489 JJ denotes Such
T8115 20490-20499 NN denotes ‘sterile’
T8116 20500-20512 NN denotes inflammation
T8117 20513-20518 MD denotes could
T8118 20519-20521 VB denotes be
T8119 20522-20531 VB denotes initiated
T8120 20532-20535 CC denotes and
T8121 20536-20540 VB denotes take
T8122 20541-20546 NN denotes place
T8123 20547-20549 IN denotes in
T8124 20550-20553 DT denotes all
T8125 20554-20560 NN denotes organs
T8126 20561-20564 CC denotes and
T8127 20565-20572 NN denotes tissues
T8128 20573-20581 VB denotes affected
T8129 20582-20584 IN denotes in
T8130 20585-20589 NN denotes cpdm
T8131 20590-20594 NN denotes mice
T8132 20594-20595 -COMMA- denotes ,
T8133 20596-20600 RB denotes thus
T8134 20601-20608 VB denotes causing
T8135 20609-20620 JJ denotes multi-organ
T8136 20621-20633 JJ denotes inflammatory
T8137 20634-20643 NN denotes disorders
T8138 20645-20649 DT denotes This
T8139 20650-20660 NN denotes hypothesis
T8140 20661-20663 VB denotes is
T8141 20664-20673 VB denotes supported
T8142 20674-20676 IN denotes by
T8143 20677-20684 JJ denotes several
T8144 20685-20691 JJ denotes recent
T8145 20692-20699 NN denotes studies
T8146 20701-20712 NN denotes Fibroblasts
T8147 20713-20715 IN denotes of
T8148 20716-20720 NN denotes cpdm
T8149 20721-20725 NN denotes mice
T8150 20726-20729 VB denotes are
T8151 20730-20736 RB denotes highly
T8152 20737-20746 JJ denotes sensitive
T8153 20747-20749 TO denotes to
T8154 20750-20762 JJ denotes TNFα-induced
T8155 20763-20767 NN denotes cell
T8156 20768-20773 NN denotes death
T8157 20774-20777 CC denotes and
T8158 20778-20781 DT denotes the
T8159 20782-20786 NN denotes cpdm
T8160 20787-20796 NN denotes phenotype
T8161 20797-20800 MD denotes can
T8162 20801-20803 VB denotes be
T8163 20804-20813 RB denotes partially
T8164 20814-20821 VB denotes rescued
T8165 20822-20824 IN denotes by
T8166 20825-20833 NN denotes deletion
T8167 20834-20836 IN denotes of
T8168 20837-20840 NN denotes TNF
T8169 20841-20842 -LRB- denotes [
T8170 20842-20843 CD denotes 7
T8171 20843-20844 -RRB- denotes ]
T8172 20844-20845 -COMMA- denotes ,
T8173 20846-20847 -LRB- denotes [
T8174 20847-20848 CD denotes 8
T8175 20848-20849 -RRB- denotes ]
T8176 20849-20850 -COMMA- denotes ,
T8177 20851-20861 VB denotes suggesting
T8178 20862-20866 IN denotes that
T8179 20867-20877 NN denotes deficiency
T8180 20878-20880 IN denotes of
T8181 20881-20888 NN denotes SHARPIN
T8182 20889-20900 VB denotes compromises
T8183 20901-20904 DT denotes the
T8184 20905-20919 JJ denotes anti-apoptotic
T8185 20920-20930 NN denotes mechanisms
T8186 20931-20933 IN denotes in
T8187 20934-20938 NN denotes cpdm
T8188 20939-20943 NN denotes mice
T8189 20944-20953 VB denotes resulting
T8190 20954-20956 IN denotes in
T8191 20957-20961 NN denotes cell
T8192 20962-20975 JJ denotes death-induced
T8193 20976-20988 JJ denotes inflammatory
T8194 20989-20996 NN denotes disease
T8195 20998-21007 NN denotes Apoptosis
T8196 21008-21010 IN denotes of
T8197 21011-21024 NN denotes keratinocytes
T8198 21025-21027 VB denotes is
T8199 21028-21029 DT denotes a
T8200 21030-21039 JJ denotes prominent
T8201 21040-21047 NN denotes feature
T8202 21048-21050 IN denotes of
T8203 21051-21054 DT denotes the
T8204 21055-21059 NN denotes skin
T8205 21060-21067 NN denotes lesions
T8206 21068-21070 IN denotes in
T8207 21071-21075 NN denotes cpdm
T8208 21076-21080 NN denotes mice
T8209 21081-21082 -LRB- denotes [
T8210 21082-21084 CD denotes 33
T8211 21084-21085 -RRB- denotes ]
T8212 21086-21089 CC denotes and
T8213 21090-21094 DT denotes this
T8214 21095-21097 VB denotes is
T8215 21098-21106 VB denotes mediated
T8216 21107-21109 IN denotes by
T8217 21110-21127 JJ denotes caspase-dependent
T8218 21128-21141 JJ denotes mitochondrial
T8219 21142-21150 NN denotes pathways
T8220 21151-21152 -LRB- denotes [
T8221 21152-21154 CD denotes 34
T8222 21154-21155 -RRB- denotes ]
T8223 21157-21162 DT denotes These
T8224 21163-21170 VB denotes induced
T8225 21171-21177 CC denotes and/or
T8226 21178-21187 JJ denotes intrinsic
T8227 21188-21197 JJ denotes apoptotic
T8228 21198-21203 NN denotes cells
T8229 21204-21207 MD denotes can
T8230 21208-21215 VB denotes release
T8231 21216-21223 JJ denotes various
T8232 21224-21229 NN denotes types
T8233 21230-21232 IN denotes of
T8234 21233-21238 NN denotes DAMPs
T8235 21239-21243 WDT denotes that
T8236 21244-21249 VB denotes exert
T8237 21250-21255 PRP-DOLLAR- denotes their
T8238 21256-21272 JJ denotes pro-inflammatory
T8239 21273-21283 NN denotes properties
T8240 21284-21286 IN denotes by
T8241 21287-21297 VB denotes activating
T8242 21298-21300 NN denotes DC
T8243 21301-21308 IN denotes through
T8244 21309-21316 NN denotes pattern
T8245 21317-21328 NN denotes recognition
T8246 21329-21338 NN denotes receptors
T8247 21339-21340 -LRB- denotes [
T8248 21340-21342 CD denotes 32
T8249 21342-21343 -RRB- denotes ]
T8250 21343-21344 -COMMA- denotes ,
T8251 21345-21346 -LRB- denotes [
T8252 21346-21348 CD denotes 35
T8253 21348-21349 -RRB- denotes ]
T8254 21349-21350 -COMMA- denotes ,
T8255 21351-21355 JJ denotes such
T8256 21356-21358 IN denotes as
T8257 21359-21364 NN denotes HMGB1
T8258 21365-21375 VB denotes recognized
T8259 21376-21378 IN denotes by
T8260 21379-21385 NN denotes TLR2/4
T8261 21386-21387 -LRB- denotes [
T8262 21387-21389 CD denotes 36
T8263 21389-21390 -RRB- denotes ]
T8264 21390-21391 -COMMA- denotes ,
T8265 21392-21393 -LRB- denotes [
T8266 21393-21395 CD denotes 37
T8267 21395-21396 -RRB- denotes ]
T8268 21398-21408 JJ denotes Consistent
T8269 21409-21413 IN denotes with
T8270 21414-21417 DT denotes the
T8271 21418-21426 JJ denotes impaired
T8272 21427-21430 NN denotes Th1
T8273 21431-21437 JJ denotes immune
T8274 21438-21446 NN denotes response
T8275 21447-21449 IN denotes in
T8276 21450-21454 NN denotes cpdm
T8277 21455-21459 NN denotes mice
T8278 21460-21461 -LRB- denotes [
T8279 21461-21462 CD denotes 5
T8280 21462-21463 -RRB- denotes ]
T8281 21463-21464 -COMMA- denotes ,
T8282 21465-21475 VB denotes stimulated
T8283 21476-21480 NN denotes BMDC
T8284 21481-21487 RB denotes weakly
T8285 21488-21497 VB denotes polarized
T8286 21498-21501 NN denotes Th1
T8287 21502-21517 NN denotes differentiation
T8288 21517-21518 -COMMA- denotes ,
T8289 21519-21522 CC denotes but
T8290 21523-21531 RB denotes strongly
T8291 21532-21541 VB denotes supported
T8292 21542-21545 DT denotes the
T8293 21546-21557 NN denotes development
T8294 21558-21560 IN denotes of
T8295 21561-21564 NN denotes Th2
T8296 21565-21573 NN denotes effector
T8297 21574-21579 NN denotes cells
T8298 21581-21589 VB denotes Combined
T8299 21590-21594 IN denotes with
T8300 21595-21598 DT denotes the
T8301 21599-21607 JJ denotes dramatic
T8302 21608-21614 NN denotes effect
T8303 21615-21617 IN denotes of
T8304 21618-21622 NN denotes IL12
T8305 21623-21632 NN denotes treatment
T8306 21633-21635 IN denotes on
T8307 21636-21639 DT denotes the
T8308 21640-21649 NN denotes phenotype
T8309 21650-21652 IN denotes of
T8310 21653-21658 DT denotes these
T8311 21659-21663 NN denotes mice
T8312 21664-21665 -LRB- denotes [
T8313 21665-21666 CD denotes 5
T8314 21666-21667 -RRB- denotes ]
T8315 21667-21668 -COMMA- denotes ,
T8316 21669-21673 DT denotes this
T8317 21674-21682 VB denotes suggests
T8318 21683-21687 IN denotes that
T8319 21688-21691 DT denotes the
T8320 21692-21702 JJ denotes Th2-biased
T8321 21703-21711 JJ denotes systemic
T8322 21712-21724 NN denotes inflammation
T8323 21725-21727 IN denotes in
T8324 21728-21732 NN denotes cpdm
T8325 21733-21737 NN denotes mice
T8326 21738-21740 VB denotes is
T8327 21741-21747 VB denotes caused
T8328 21748-21750 IN denotes by
T8329 21751-21758 VB denotes reduced
T8330 21759-21766 NN denotes IL12P70
T8331 21767-21777 NN denotes production
T8332 21778-21782 IN denotes from
T8333 21783-21785 NN denotes DC
T8334 21787-21790 DT denotes The
T8335 21791-21801 NN denotes importance
T8336 21802-21804 IN denotes of
T8337 21805-21812 NN denotes IL12P70
T8338 21813-21815 IN denotes in
T8339 21816-21826 VB denotes regulating
T8340 21827-21830 NN denotes Th1
T8341 21831-21834 CC denotes and
T8342 21835-21838 NN denotes Th2
T8343 21839-21845 JJ denotes immune
T8344 21846-21855 NN denotes responses
T8345 21856-21858 IN denotes in
T8346 21859-21863 NN denotes mice
T8347 21864-21867 VB denotes was
T8348 21868-21875 RB denotes clearly
T8349 21876-21888 VB denotes demonstrated
T8350 21889-21891 IN denotes in
T8351 21892-21900 NN denotes IL12P35-
T8352 21901-21904 CC denotes and
T8353 21905-21922 JJ denotes IL12P40-deficient
T8354 21923-21927 NN denotes mice
T8355 21928-21929 -LRB- denotes [
T8356 21929-21931 CD denotes 38
T8357 21931-21932 -RRB- denotes ]
T8358 21932-21933 -COMMA- denotes ,
T8359 21934-21935 -LRB- denotes [
T8360 21935-21937 CD denotes 39
T8361 21937-21938 -RRB- denotes ]
T8362 21939-21942 CC denotes and
T8363 21943-21950 IN denotes through
T8364 21951-21959 JJ denotes clinical
T8365 21960-21967 NN denotes studies
T8366 21968-21970 IN denotes in
T8367 21971-21976 JJ denotes human
T8368 21977-21985 NN denotes patients
T8369 21986-21987 -LRB- denotes [
T8370 21987-21989 CD denotes 40
T8371 21989-21990 -RRB- denotes ]
T8372 21990-21991 CD denotes –
T8373 21991-21992 -LRB- denotes [
T8374 21992-21994 CD denotes 42
T8375 21994-21995 -RRB- denotes ]
T8376 21997-22002 NN denotes NF-κB
T8377 22002-22003 -COMMA- denotes ,
T8378 22004-22012 NN denotes TBK/IRF3
T8379 22012-22013 -COMMA- denotes ,
T8380 22014-22017 CC denotes and
T8381 22018-22022 NN denotes MAPK
T8382 22023-22032 NN denotes signaling
T8383 22033-22036 VB denotes are
T8384 22037-22046 JJ denotes important
T8385 22047-22055 NN denotes pathways
T8386 22056-22065 VB denotes activated
T8387 22066-22068 IN denotes by
T8388 22069-22072 NN denotes LPS
T8389 22073-22075 CC denotes or
T8390 22076-22080 NN denotes poly
T8391 22081-22082 NN denotes I
T8392 22082-22083 -COLON- denotes :
T8393 22083-22084 NN denotes C
T8394 22085-22088 CC denotes and
T8395 22089-22099 NN denotes disruption
T8396 22100-22102 IN denotes of
T8397 22103-22109 DT denotes either
T8398 22110-22117 NN denotes pathway
T8399 22118-22121 MD denotes may
T8400 22122-22126 VB denotes lead
T8401 22127-22129 TO denotes to
T8402 22130-22139 VB denotes decreased
T8403 22140-22148 NN denotes cytokine
T8404 22149-22159 NN denotes expression
T8405 22161-22166 NN denotes NF-κB
T8406 22167-22177 NN denotes activation
T8407 22178-22181 VB denotes was
T8408 22182-22193 RB denotes selectively
T8409 22194-22203 VB denotes inhibited
T8410 22204-22206 IN denotes in
T8411 22207-22210 NN denotes LPS
T8412 22211-22214 CC denotes and
T8413 22215-22219 NN denotes poly
T8414 22220-22221 NN denotes I
T8415 22221-22222 SYM denotes :
T8416 22222-22223 NN denotes C
T8417 22224-22234 VB denotes stimulated
T8418 22235-22239 NN denotes cpdm
T8419 22240-22244 NN denotes BMDC
T8420 22245-22253 VB denotes compared
T8421 22254-22256 TO denotes to
T8422 22257-22261 JJ denotes wild
T8423 22262-22266 NN denotes type
T8424 22266-22267 -COMMA- denotes ,
T8425 22268-22273 IN denotes while
T8426 22274-22284 NN denotes activation
T8427 22285-22287 IN denotes of
T8428 22288-22297 NN denotes TBK1/IRF3
T8429 22297-22298 -COMMA- denotes ,
T8430 22299-22305 NN denotes ERK1/2
T8431 22305-22306 -COMMA- denotes ,
T8432 22307-22309 CC denotes or
T8433 22310-22313 NN denotes p38
T8434 22314-22317 VB denotes was
T8435 22318-22321 RB denotes not
T8436 22321-22322 -COMMA- denotes ,
T8437 22323-22333 VB denotes indicating
T8438 22334-22338 IN denotes that
T8439 22339-22348 VB denotes disrupted
T8440 22349-22354 NN denotes NF-κB
T8441 22355-22364 NN denotes signaling
T8442 22365-22367 IN denotes in
T8443 22368-22372 NN denotes cpdm
T8444 22373-22375 NN denotes DC
T8445 22376-22378 VB denotes is
T8446 22379-22390 JJ denotes responsible
T8447 22391-22394 IN denotes for
T8448 22395-22398 DT denotes the
T8449 22399-22408 JJ denotes defective
T8450 22409-22417 NN denotes cytokine
T8451 22418-22428 NN denotes expression
T8452 22430-22435 DT denotes These
T8453 22436-22443 NN denotes results
T8454 22444-22448 VB denotes seem
T8455 22449-22451 TO denotes to
T8456 22452-22462 VB denotes contradict
T8457 22463-22464 DT denotes a
T8458 22465-22471 JJ denotes recent
T8459 22472-22477 NN denotes study
T8460 22478-22482 WDT denotes that
T8461 22483-22488 VB denotes found
T8462 22489-22498 VB denotes increased
T8463 22499-22505 NN denotes levels
T8464 22506-22508 IN denotes of
T8465 22509-22514 NN denotes NF-κB
T8466 22515-22525 NN denotes activation
T8467 22526-22529 CC denotes and
T8468 22530-22533 NN denotes IL1
T8469 22534-22547 NN denotes transcription
T8470 22548-22550 IN denotes in
T8471 22551-22555 NN denotes cpdm
T8472 22556-22560 NN denotes mice
T8473 22561-22562 -LRB- denotes [
T8474 22562-22564 CD denotes 43
T8475 22564-22565 -RRB- denotes ]
T8476 22567-22571 DT denotes This
T8477 22572-22582 NN denotes difference
T8478 22583-22586 MD denotes can
T8479 22587-22595 RB denotes probably
T8480 22596-22598 VB denotes be
T8481 22599-22609 VB denotes attributed
T8482 22610-22612 TO denotes to
T8483 22613-22616 DT denotes the
T8484 22617-22626 JJ denotes different
T8485 22627-22632 NN denotes cells
T8486 22633-22636 CC denotes and
T8487 22637-22644 NN denotes tissues
T8488 22645-22649 VB denotes used
T8489 22650-22652 IN denotes in
T8490 22653-22658 DT denotes these
T8491 22659-22666 NN denotes studies
T8492 22666-22667 -COMMA- denotes ,
T8493 22668-22671 CC denotes and
T8494 22672-22675 MD denotes may
T8495 22676-22681 VB denotes point
T8496 22682-22684 TO denotes to
T8497 22685-22690 NN denotes cell-
T8498 22691-22694 CC denotes and
T8499 22695-22702 NN denotes tissue-
T8500 22703-22707 NN denotes type
T8501 22708-22716 JJ denotes specific
T8502 22717-22726 NN denotes functions
T8503 22727-22729 IN denotes of
T8504 22730-22737 NN denotes SHARPIN
T8505 22739-22743 DT denotes This
T8506 22744-22746 VB denotes is
T8507 22747-22757 JJ denotes consistent
T8508 22758-22762 IN denotes with
T8509 22763-22769 JJ denotes recent
T8510 22770-22777 NN denotes reports
T8511 22778-22780 IN denotes of
T8512 22781-22796 JJ denotes tissue-specific
T8513 22797-22804 NN denotes effects
T8514 22805-22807 IN denotes of
T8515 22808-22813 NN denotes NF-κB
T8516 22814-22823 NN denotes signaling
T8517 22824-22825 -LRB- denotes [
T8518 22825-22827 CD denotes 44
T8519 22827-22828 -RRB- denotes ]
T8520 22830-22840 JJ denotes Ubiquitous
T8521 22841-22851 NN denotes activation
T8522 22852-22854 IN denotes of
T8523 22855-22860 NN denotes NF-κB
T8524 22861-22863 IN denotes by
T8525 22864-22872 VB denotes removing
T8526 22873-22883 NN denotes inhibitors
T8527 22884-22888 JJ denotes such
T8528 22889-22891 IN denotes as
T8529 22892-22895 NN denotes A20
T8530 22896-22899 CC denotes and
T8531 22900-22904 NN denotes ITCH
T8532 22905-22912 IN denotes through
T8533 22913-22920 JJ denotes genetic
T8534 22921-22933 NN denotes manipulation
T8535 22934-22941 VB denotes results
T8536 22942-22944 IN denotes in
T8537 22945-22955 JJ denotes widespread
T8538 22956-22968 NN denotes inflammation
T8539 22968-22969 -COMMA- denotes ,
T8540 22970-22980 JJ denotes consistent
T8541 22981-22985 IN denotes with
T8542 22986-22989 DT denotes the
T8543 22990-22994 NN denotes role
T8544 22995-22997 IN denotes of
T8545 22998-23003 NN denotes NF-κB
T8546 23004-23006 IN denotes in
T8547 23007-23010 DT denotes the
T8548 23011-23021 NN denotes production
T8549 23022-23024 IN denotes of
T8550 23025-23041 JJ denotes pro-inflammatory
T8551 23042-23051 NN denotes mediators
T8552 23052-23053 -LRB- denotes [
T8553 23053-23055 CD denotes 45
T8554 23055-23056 -RRB- denotes ]
T8555 23056-23057 -COMMA- denotes ,
T8556 23058-23059 -LRB- denotes [
T8557 23059-23061 CD denotes 46
T8558 23061-23062 -RRB- denotes ]
T8559 23064-23071 RB denotes However
T8560 23071-23072 -COMMA- denotes ,
T8561 23073-23082 JJ denotes selective
T8562 23083-23093 NN denotes inhibition
T8563 23094-23096 IN denotes of
T8564 23097-23102 NN denotes NF-κB
T8565 23103-23113 NN denotes activation
T8566 23114-23116 IN denotes in
T8567 23117-23128 JJ denotes parenchymal
T8568 23129-23134 NN denotes cells
T8569 23135-23137 IN denotes of
T8570 23138-23141 DT denotes the
T8571 23142-23146 NN denotes skin
T8572 23146-23147 -COMMA- denotes ,
T8573 23148-23153 NN denotes liver
T8574 23153-23154 -COMMA- denotes ,
T8575 23155-23158 CC denotes and
T8576 23159-23168 NN denotes intestine
T8577 23169-23176 VB denotes results
T8578 23177-23179 IN denotes in
T8579 23180-23187 JJ denotes chronic
T8580 23188-23200 NN denotes inflammation
T8581 23201-23207 VB denotes driven
T8582 23208-23210 IN denotes by
T8583 23211-23216 NN denotes NF-κB
T8584 23217-23226 JJ denotes competent
T8585 23227-23237 NN denotes leukocytes
T8586 23238-23239 -LRB- denotes [
T8587 23239-23241 CD denotes 47
T8588 23241-23242 -RRB- denotes ]
T8589 23242-23243 CD denotes –
T8590 23243-23244 -LRB- denotes [
T8591 23244-23246 CD denotes 50
T8592 23246-23247 -RRB- denotes ]
T8593 23249-23253 DT denotes This
T8594 23254-23263 VB denotes indicates
T8595 23264-23268 IN denotes that
T8596 23269-23270 DT denotes a
T8597 23271-23278 NN denotes balance
T8598 23279-23286 IN denotes between
T8599 23287-23290 DT denotes the
T8600 23291-23307 JJ denotes pro-inflammatory
T8601 23308-23312 NN denotes role
T8602 23313-23315 IN denotes of
T8603 23316-23321 NN denotes NF-κB
T8604 23322-23324 IN denotes in
T8605 23325-23335 NN denotes leukocytes
T8606 23336-23339 CC denotes and
T8607 23340-23343 DT denotes the
T8608 23344-23361 JJ denotes anti-inflammatory
T8609 23362-23366 NN denotes role
T8610 23367-23369 IN denotes in
T8611 23370-23381 JJ denotes parenchymal
T8612 23382-23387 NN denotes cells
T8613 23388-23390 VB denotes is
T8614 23391-23399 JJ denotes critical
T8615 23400-23402 IN denotes in
T8616 23403-23406 DT denotes the
T8617 23407-23418 NN denotes maintenance
T8618 23419-23421 IN denotes of
T8619 23422-23428 NN denotes tissue
T8620 23429-23440 NN denotes homeostasis
T8621 23442-23453 NN denotes Experiments
T8622 23454-23458 IN denotes with
T8623 23459-23463 NN denotes mice
T8624 23464-23468 IN denotes with
T8625 23469-23474 NN denotes cell-
T8626 23475-23478 CC denotes and
T8627 23479-23494 JJ denotes tissue-specific
T8628 23495-23503 NN denotes deletion
T8629 23504-23506 IN denotes of
T8630 23507-23514 NNP denotes Sharpin
T8631 23514-23515 -COMMA- denotes ,
T8632 23516-23525 RB denotes currently
T8633 23526-23531 IN denotes under
T8634 23532-23535 NN denotes way
T8635 23535-23536 -COMMA- denotes ,
T8636 23537-23541 MD denotes will
T8637 23542-23546 VB denotes help
T8638 23547-23549 TO denotes to
T8639 23550-23559 VB denotes elucidate
T8640 23560-23563 DT denotes the
T8641 23564-23568 NN denotes role
T8642 23569-23571 IN denotes of
T8643 23572-23579 NN denotes SHARPIN
T8644 23580-23582 IN denotes in
T8645 23583-23595 NN denotes inflammation
T8646 23597-23604 IN denotes Despite
T8647 23605-23614 JJ denotes defective
T8648 23615-23620 NN denotes NF-κB
T8649 23621-23631 NN denotes activation
T8650 23632-23634 IN denotes in
T8651 23635-23638 DT denotes the
T8652 23639-23646 NN denotes absence
T8653 23647-23649 IN denotes of
T8654 23650-23657 NN denotes Sharpin
T8655 23658-23668 NN denotes expression
T8656 23668-23669 -COMMA- denotes ,
T8657 23670-23675 EX denotes there
T8658 23676-23679 VB denotes was
T8659 23680-23682 DT denotes no
T8660 23683-23694 JJ denotes significant
T8661 23695-23701 NN denotes change
T8662 23702-23704 IN denotes in
T8663 23705-23712 JJ denotes splenic
T8664 23713-23715 NN denotes DC
T8665 23716-23727 NN denotes populations
T8666 23728-23730 CC denotes or
T8667 23731-23741 NN denotes expression
T8668 23742-23744 IN denotes of
T8669 23745-23759 JJ denotes co-stimulatory
T8670 23760-23769 NN denotes molecules
T8671 23770-23772 IN denotes on
T8672 23773-23777 NN denotes BMDC
T8673 23779-23788 JJ denotes Different
T8674 23789-23794 NN denotes NF-κB
T8675 23795-23803 NN denotes subunits
T8676 23804-23812 VB denotes involved
T8677 23813-23815 IN denotes in
T8678 23816-23819 DT denotes the
T8679 23820-23829 JJ denotes canonical
T8680 23830-23833 CC denotes and
T8681 23834-23847 JJ denotes non-canonical
T8682 23848-23856 NN denotes branches
T8683 23857-23859 IN denotes of
T8684 23860-23863 DT denotes the
T8685 23864-23869 NN denotes NF-κB
T8686 23870-23879 NN denotes signaling
T8687 23880-23887 NN denotes pathway
T8688 23888-23892 VB denotes have
T8689 23893-23901 JJ denotes distinct
T8690 23902-23911 NN denotes functions
T8691 23912-23914 TO denotes to
T8692 23915-23922 VB denotes control
T8693 23923-23931 JJ denotes specific
T8694 23932-23939 NN denotes aspects
T8695 23940-23942 IN denotes of
T8696 23943-23945 NN denotes DC
T8697 23946-23957 NN denotes development
T8698 23958-23961 CC denotes and
T8699 23962-23970 NN denotes function
T8700 23971-23972 -LRB- denotes [
T8701 23972-23974 CD denotes 51
T8702 23974-23975 -RRB- denotes ]
T8703 23975-23976 -COMMA- denotes ,
T8704 23977-23978 -LRB- denotes [
T8705 23978-23980 CD denotes 52
T8706 23980-23981 -RRB- denotes ]
T8707 23983-23986 DT denotes The
T8708 23987-24000 JJ denotes non-canonical
T8709 24001-24008 NN denotes pathway
T8710 24009-24010 -LRB- denotes (
T8711 24010-24014 NN denotes p100
T8712 24015-24025 NN denotes processing
T8713 24026-24028 TO denotes to
T8714 24029-24036 VB denotes produce
T8715 24037-24040 NN denotes p52
T8716 24040-24041 -RRB- denotes )
T8717 24042-24049 VB denotes appears
T8718 24050-24052 TO denotes to
T8719 24053-24055 VB denotes be
T8720 24056-24062 JJ denotes intact
T8721 24063-24065 IN denotes in
T8722 24066-24083 JJ denotes SHARPIN-deficient
T8723 24084-24089 NN denotes cells
T8724 24090-24091 -LRB- denotes [
T8725 24091-24092 CD denotes 7
T8726 24092-24093 -RRB- denotes ]
T8727 24093-24094 -COMMA- denotes ,
T8728 24095-24096 -LRB- denotes [
T8729 24096-24097 CD denotes 9
T8730 24097-24098 -RRB- denotes ]
T8731 24099-24109 VB denotes suggesting
T8732 24110-24114 IN denotes that
T8733 24115-24118 DT denotes the
T8734 24119-24124 NN denotes NF-κB
T8735 24125-24136 NN denotes heterodimer
T8736 24137-24145 NN denotes p52/RELB
T8737 24146-24148 VB denotes is
T8738 24149-24159 JJ denotes sufficient
T8739 24160-24162 TO denotes to
T8740 24163-24171 VB denotes maintain
T8741 24172-24175 DT denotes the
T8742 24176-24182 JJ denotes normal
T8743 24183-24193 NN denotes regulation
T8744 24194-24196 IN denotes of
T8745 24197-24199 NN denotes DC
T8746 24200-24211 NN denotes homeostasis
T8747 24212-24215 CC denotes and
T8748 24216-24226 NN denotes maturation
T8749 24228-24231 DT denotes The
T8750 24232-24241 JJ denotes molecular
T8751 24242-24247 NN denotes basis
T8752 24248-24250 IN denotes by
T8753 24251-24256 WDT denotes which
T8754 24257-24260 DT denotes the
T8755 24261-24268 NNP denotes Sharpin
T8756 24269-24277 NN denotes mutation
T8757 24278-24284 VB denotes causes
T8758 24285-24292 VB denotes reduced
T8759 24293-24298 NN denotes NF-κB
T8760 24299-24309 NN denotes activation
T8761 24310-24312 IN denotes in
T8762 24313-24317 NN denotes BMDC
T8763 24318-24325 VB denotes remains
T8764 24326-24328 TO denotes to
T8765 24329-24331 VB denotes be
T8766 24332-24342 VB denotes determined
T8767 24344-24347 NN denotes LPS
T8768 24348-24351 CC denotes and
T8769 24352-24356 NN denotes poly
T8770 24357-24358 NN denotes I
T8771 24358-24359 -COLON- denotes :
T8772 24359-24360 NN denotes C
T8773 24361-24365 VB denotes used
T8774 24366-24370 RB denotes here
T8775 24371-24374 VB denotes are
T8776 24375-24387 JJ denotes well-defined
T8777 24388-24395 NN denotes ligands
T8778 24396-24400 WDT denotes that
T8779 24401-24413 RB denotes specifically
T8780 24414-24420 VB denotes engage
T8781 24421-24425 NN denotes TLR4
T8782 24426-24429 CC denotes and
T8783 24430-24434 NN denotes TLR3
T8784 24434-24435 -COMMA- denotes ,
T8785 24436-24448 RB denotes respectively
T8786 24450-24453 DT denotes The
T8787 24454-24464 NN denotes expression
T8788 24465-24472 NN denotes profile
T8789 24473-24475 IN denotes of
T8790 24476-24483 NN denotes surface
T8791 24484-24488 NN denotes TLR4
T8792 24489-24498 NN denotes complexes
T8793 24499-24501 VB denotes is
T8794 24502-24509 JJ denotes similar
T8795 24510-24517 IN denotes between
T8796 24518-24520 NN denotes WT
T8797 24521-24524 CC denotes and
T8798 24525-24529 NN denotes cpdm
T8799 24530-24534 NN denotes BMDC
T8800 24535-24545 VB denotes suggesting
T8801 24546-24550 IN denotes that
T8802 24551-24560 JJ denotes defective
T8803 24561-24566 NN denotes NF-κB
T8804 24567-24577 NN denotes activation
T8805 24578-24580 VB denotes is
T8806 24581-24584 RB denotes not
T8807 24585-24586 DT denotes a
T8808 24587-24593 NN denotes result
T8809 24594-24596 IN denotes of
T8810 24597-24609 JJ denotes differential
T8811 24610-24613 NN denotes TLR
T8812 24614-24624 NN denotes expression
T8813 24625-24627 IN denotes on
T8814 24628-24634 NN denotes target
T8815 24635-24640 NN denotes cells
T8816 24642-24645 NN denotes LPS
T8817 24646-24653 VB denotes engages
T8818 24654-24658 NN denotes TLR4
T8819 24659-24661 TO denotes to
T8820 24662-24670 VB denotes activate
T8821 24671-24686 JJ denotes MYD88-dependent
T8822 24687-24690 CC denotes and
T8823 24691-24705 JJ denotes TRIF-dependent
T8824 24706-24714 NN denotes pathways
T8825 24714-24715 -COMMA- denotes ,
T8826 24716-24723 IN denotes whereas
T8827 24724-24728 NN denotes TLR3
T8828 24729-24739 VB denotes stimulated
T8829 24740-24742 IN denotes by
T8830 24743-24747 NN denotes poly
T8831 24748-24749 NN denotes I
T8832 24749-24750 -COLON- denotes :
T8833 24750-24751 NN denotes C
T8834 24752-24756 RB denotes only
T8835 24757-24765 VB denotes triggers
T8836 24766-24780 JJ denotes TRIF-dependent
T8837 24781-24790 NN denotes signaling
T8838 24791-24792 -LRB- denotes [
T8839 24792-24794 CD denotes 53
T8840 24794-24795 -RRB- denotes ]
T8841 24797-24800 DT denotes The
T8842 24801-24810 JJ denotes defective
T8843 24811-24816 NN denotes NF-κB
T8844 24817-24827 NN denotes activation
T8845 24828-24830 IN denotes by
T8846 24831-24835 DT denotes both
T8847 24836-24843 NN denotes stimuli
T8848 24844-24852 VB denotes suggests
T8849 24853-24857 IN denotes that
T8850 24858-24861 DT denotes the
T8851 24862-24869 NNP denotes Sharpin
T8852 24870-24878 NN denotes mutation
T8853 24879-24889 VB denotes interferes
T8854 24890-24894 IN denotes with
T8855 24895-24898 DT denotes the
T8856 24899-24906 NN denotes protein
T8857 24907-24915 NN denotes adaptors
T8858 24916-24918 CC denotes or
T8859 24919-24926 NN denotes kinases
T8860 24927-24933 VB denotes shared
T8861 24934-24936 IN denotes by
T8862 24937-24941 CC denotes both
T8863 24942-24951 NN denotes signaling
T8864 24952-24960 NN denotes pathways
T8865 24960-24961 -COMMA- denotes ,
T8866 24962-24966 JJ denotes such
T8867 24967-24969 IN denotes as
T8868 24970-24974 NN denotes RIP1
T8869 24975-24978 CC denotes and
T8870 24979-24984 NN denotes TRAF6
T8871 24985-24986 -LRB- denotes [
T8872 24986-24988 CD denotes 18
T8873 24988-24989 -RRB- denotes ]
T8874 24991-24997 JJ denotes Recent
T8875 24998-25005 NN denotes studies
T8876 25006-25018 VB denotes demonstrated
T8877 25019-25023 IN denotes that
T8878 25024-25031 NN denotes SHARPIN
T8879 25032-25041 VB denotes interacts
T8880 25042-25046 IN denotes with
T8881 25047-25051 NN denotes HOIP
T8882 25052-25054 TO denotes to
T8883 25055-25059 VB denotes form
T8884 25060-25065 NN denotes LUBAC
T8885 25066-25070 WDT denotes that
T8886 25071-25077 VB denotes exerts
T8887 25078-25081 PRP-DOLLAR- denotes its
T8888 25082-25111 NN denotes linear-ubiquitin-chain-ligase
T8889 25112-25120 NN denotes activity
T8890 25121-25123 IN denotes on
T8891 25124-25129 NN denotes NF-κB
T8892 25130-25139 NN denotes signaling
T8893 25140-25147 NN denotes players
T8894 25148-25152 NN denotes RIP1
T8895 25153-25156 CC denotes and
T8896 25157-25161 NN denotes NEMO
T8897 25162-25163 -LRB- denotes [
T8898 25163-25164 CD denotes 7
T8899 25164-25165 -RRB- denotes ]
T8900 25165-25166 -COMMA- denotes ,
T8901 25167-25169 DT denotes an
T8902 25170-25179 JJ denotes essential
T8903 25180-25184 NN denotes step
T8904 25185-25188 IN denotes for
T8905 25189-25195 JJ denotes intact
T8906 25196-25211 JJ denotes TNFα-stimulated
T8907 25212-25217 NN denotes NF-κB
T8908 25218-25228 NN denotes activation
T8909 25230-25233 DT denotes The
T8910 25234-25241 NNP denotes Sharpin
T8911 25242-25246 JJ denotes null
T8912 25247-25255 NN denotes mutation
T8913 25256-25264 VB denotes disrupts
T8914 25265-25268 DT denotes the
T8915 25269-25283 NN denotes ubiquitylation
T8916 25284-25291 NN denotes process
T8917 25292-25295 CC denotes and
T8918 25296-25305 VB denotes abrogates
T8919 25306-25309 DT denotes the
T8920 25310-25322 JJ denotes TNFα-induced
T8921 25323-25328 NN denotes NF-κB
T8922 25329-25338 NN denotes signaling
T8923 25339-25346 NN denotes pathway
T8924 25348-25353 IN denotes Since
T8925 25354-25358 NN denotes TNFR
T8926 25359-25362 CC denotes and
T8927 25363-25366 NN denotes TLR
T8928 25367-25376 RB denotes partially
T8929 25377-25382 VB denotes share
T8930 25383-25388 PRP-DOLLAR- denotes their
T8931 25389-25399 JJ denotes downstream
T8932 25400-25409 NN denotes signaling
T8933 25410-25418 NN denotes cascades
T8934 25418-25419 -COMMA- denotes ,
T8935 25420-25421 DT denotes a
T8936 25422-25429 JJ denotes similar
T8937 25430-25448 JJ denotes ubiquitin-mediated
T8938 25449-25459 NN denotes regulation
T8939 25460-25463 MD denotes may
T8940 25464-25468 VB denotes hold
T8941 25469-25473 JJ denotes true
T8942 25474-25477 IN denotes for
T8943 25478-25485 NN denotes SHARPIN
T8944 25486-25488 IN denotes in
T8945 25489-25493 NN denotes LPS-
T8946 25494-25497 CC denotes and
T8947 25498-25502 NN denotes poly
T8948 25503-25504 CD denotes I
T8949 25504-25505 -COLON- denotes :
T8950 25505-25514 JJ denotes C-induced
T8951 25515-25520 NN denotes NF-κB
T8952 25521-25531 NN denotes activation
T8953 25533-25535 IN denotes In
T8954 25536-25543 NN denotes summary
T8955 25543-25544 -COMMA- denotes ,
T8956 25545-25548 DT denotes the
T8957 25549-25556 JJ denotes present
T8958 25557-25562 NN denotes study
T8959 25563-25573 VB denotes identified
T8960 25574-25576 DT denotes an
T8961 25577-25590 JJ denotes indispensible
T8962 25591-25595 NN denotes role
T8963 25596-25598 IN denotes of
T8964 25599-25606 NN denotes SHARPIN
T8965 25607-25609 IN denotes in
T8966 25610-25613 DT denotes the
T8967 25614-25624 NN denotes production
T8968 25625-25627 IN denotes of
T8969 25628-25644 JJ denotes pro-inflammatory
T8970 25645-25654 NN denotes mediators
T8971 25655-25658 CC denotes and
T8972 25659-25670 JJ denotes TLR-induced
T8973 25671-25676 NN denotes NF-κB
T8974 25677-25686 NN denotes signaling
T8975 25688-25691 DT denotes The
T8976 25692-25700 JJ denotes impaired
T8977 25701-25716 JJ denotes Th1-stimulating
T8978 25717-25724 NN denotes ability
T8979 25725-25727 IN denotes of
T8980 25728-25745 JJ denotes Sharpin-deficient
T8981 25746-25748 NN denotes DC
T8982 25749-25752 MD denotes may
T8983 25753-25760 VB denotes account
T8984 25761-25764 IN denotes for
T8985 25765-25768 DT denotes the
T8986 25769-25781 JJ denotes Th2-dominant
T8987 25782-25794 JJ denotes inflammatory
T8988 25795-25804 NN denotes phenotype
T8989 25805-25807 IN denotes of
T8990 25808-25812 NN denotes cpdm
T8991 25813-25817 NN denotes mice
T8992 25819-25822 DT denotes The
T8993 25823-25830 NN denotes balance
T8994 25831-25838 IN denotes between
T8995 25839-25842 NN denotes Th1
T8996 25843-25846 CC denotes and
T8997 25847-25850 NN denotes Th2
T8998 25851-25866 NN denotes differentiation
T8999 25867-25869 VB denotes is
T9000 25870-25878 JJ denotes critical
T9001 25879-25882 IN denotes for
T9002 25883-25889 JJ denotes immune
T9003 25890-25901 NN denotes homeostasis
T9004 25903-25904 DT denotes A
T9005 25905-25911 JJ denotes better
T9006 25912-25925 NN denotes understanding
T9007 25926-25928 IN denotes of
T9008 25929-25932 WRB denotes how
T9009 25933-25937 JJ denotes such
T9010 25938-25945 NN denotes balance
T9011 25946-25948 VB denotes is
T9012 25949-25959 VB denotes maintained
T9013 25960-25964 MD denotes will
T9014 25965-25969 VB denotes help
T9015 25970-25976 VB denotes design
T9016 25977-25985 NN denotes cytokine
T9017 25986-25995 NN denotes treatment
T9018 25996-25999 IN denotes for
T9019 26000-26005 JJ denotes human
T9020 26006-26014 NN denotes diseases
T9021 26015-26019 IN denotes with
T9022 26020-26030 JJ denotes Th2-biased
T9023 26031-26039 NN denotes cytokine
T9024 26040-26049 NN denotes secretion
T9025 26050-26057 JJ denotes similar
T9026 26058-26060 TO denotes to
T9027 26061-26064 DT denotes the
T9028 26065-26070 NN denotes mouse
T9029 26071-26075 NN denotes cpdm
T9030 26075-26076 -COMMA- denotes ,
T9031 26077-26081 JJ denotes such
T9032 26082-26084 IN denotes as
T9033 26085-26094 NN denotes allergies
T9034 26095-26098 CC denotes and
T9035 26099-26116 JJ denotes hypereosinophilic
T9036 26117-26126 NN denotes syndromes
T9786 26299-26303 NN denotes Mice
T9787 26304-26321 NN denotes Specific-pathogen
T9788 26322-26326 JJ denotes free
T9789 26327-26335 NN denotes colonies
T9790 26336-26338 IN denotes of
T9791 26339-26373 NN denotes C57BL/KaLawRij-Sharpincpdm/RijSunJ
T9792 26374-26375 -LRB- denotes (
T9793 26375-26377 NNP denotes JR
T9794 26377-26378 -SHARP- denotes #
T9795 26378-26382 CD denotes 7599
T9796 26382-26383 -RRB- denotes )
T9797 26384-26387 CC denotes and
T9798 26388-26390 JJ denotes WT
T9799 26391-26395 NN denotes mice
T9800 26396-26400 VB denotes were
T9801 26401-26409 VB denotes obtained
T9802 26410-26414 IN denotes from
T9803 26415-26418 DT denotes The
T9804 26419-26426 NNP denotes Jackson
T9805 26427-26437 NNP denotes Laboratory
T9806 26438-26439 -LRB- denotes (
T9807 26439-26442 NNP denotes Bar
T9808 26443-26449 NNP denotes Harbor
T9809 26449-26450 -COMMA- denotes ,
T9810 26451-26453 NN denotes ME
T9811 26453-26454 -RRB- denotes )
T9812 26455-26458 CC denotes and
T9813 26459-26463 VB denotes were
T9814 26464-26474 VB denotes maintained
T9815 26475-26477 IN denotes in
T9816 26478-26479 DT denotes a
T9817 26480-26487 NN denotes barrier
T9818 26488-26496 NN denotes facility
T9819 26498-26504 JJ denotes Normal
T9820 26505-26515 NN denotes littermate
T9821 26516-26524 NN denotes controls
T9822 26525-26529 VB denotes were
T9823 26530-26536 CC denotes either
T9824 26537-26540 SYM denotes +/+
T9825 26541-26543 CC denotes or
T9826 26544-26558 NNP denotes +/Sharpincpdm.
T9827 26559-26564 DT denotes These
T9828 26565-26572 NN denotes control
T9829 26573-26580 NN denotes animals
T9830 26581-26585 VB denotes were
T9831 26586-26600 RB denotes phenotypically
T9832 26601-26618 JJ denotes indistinguishable
T9833 26619-26622 CC denotes and
T9834 26623-26626 VB denotes are
T9835 26627-26635 VB denotes referred
T9836 26636-26638 TO denotes to
T9837 26639-26641 IN denotes as
T9838 26642-26644 NNP denotes WT
T9839 26646-26657 JJ denotes Sex-matched
T9840 26658-26660 NN denotes WT
T9841 26661-26664 CC denotes and
T9842 26665-26671 JJ denotes mutant
T9843 26672-26676 NN denotes mice
T9844 26677-26681 VB denotes were
T9845 26682-26686 VB denotes used
T9846 26687-26689 IN denotes at
T9847 26690-26694 CD denotes 6–10
T9848 26695-26700 NN denotes weeks
T9849 26701-26703 IN denotes of
T9850 26704-26707 NN denotes age
T9851 26709-26712 IN denotes For
T9852 26713-26717 DT denotes some
T9853 26718-26729 NN denotes experiments
T9854 26729-26730 -COMMA- denotes ,
T9855 26731-26735 NN denotes cpdm
T9856 26736-26740 NN denotes mice
T9857 26741-26745 VB denotes were
T9858 26746-26753 VB denotes crossed
T9859 26754-26758 IN denotes with
T9860 26759-26769 JJ denotes transgenic
T9861 26770-26774 NN denotes mice
T9862 26775-26779 IN denotes with
T9863 26780-26781 DT denotes a
T9864 26782-26791 JJ denotes bacterial
T9865 26792-26802 JJ denotes artificial
T9866 26803-26813 NN denotes chromosome
T9867 26814-26815 -LRB- denotes (
T9868 26815-26818 NN denotes BAC
T9869 26818-26819 -RRB- denotes )
T9870 26820-26830 VB denotes containing
T9871 26831-26834 DT denotes the
T9872 26835-26842 NN denotes Sharpin
T9873 26843-26847 NN denotes gene
T9874 26848-26849 -LRB- denotes (
T9875 26849-26858 NN denotes FVB/NJ-Tg
T9876 26858-26859 -LRB- denotes (
T9877 26859-26870 NN denotes RP24-173I23
T9878 26870-26871 -RRB- denotes )
T9879 26871-26879 NN denotes 1Sun/Sun
T9880 26879-26880 -COMMA- denotes ,
T9881 26881-26883 NNP denotes JR
T9882 26883-26884 -SHARP- denotes #
T9883 26884-26888 CD denotes 8279
T9884 26888-26889 -RRB- denotes )
T9885 26891-26896 DT denotes These
T9886 26897-26901 NN denotes mice
T9887 26902-26906 VB denotes were
T9888 26907-26918 VB denotes backcrossed
T9889 26919-26923 IN denotes onto
T9890 26924-26927 DT denotes the
T9891 26928-26962 NN denotes C57BL/KaLawRij-Sharpincpdm/RijSunJ
T9892 26963-26973 NN denotes background
T9893 26974-26977 CC denotes and
T9894 26978-26980 NN denotes N4
T9895 26981-26985 NN denotes mice
T9896 26986-26990 VB denotes were
T9897 26991-26995 VB denotes used
T9898 26996-26998 IN denotes in
T9899 26999-27002 DT denotes the
T9900 27003-27014 NN denotes experiments
T9901 27015-27023 VB denotes reported
T9902 27024-27028 RB denotes here
T9903 27030-27033 DT denotes All
T9904 27034-27039 NN denotes mouse
T9905 27040-27044 NN denotes work
T9906 27045-27048 VB denotes was
T9907 27049-27056 VB denotes carried
T9908 27057-27060 RP denotes out
T9909 27061-27063 IN denotes in
T9910 27064-27070 JJ denotes strict
T9911 27071-27081 NN denotes accordance
T9912 27082-27086 IN denotes with
T9913 27087-27090 DT denotes the
T9914 27091-27099 VB denotes approved
T9915 27100-27109 NN denotes protocols
T9916 27110-27112 IN denotes by
T9917 27113-27116 DT denotes the
T9918 27117-27130 NNP denotes Institutional
T9919 27131-27137 NNP denotes Animal
T9920 27138-27142 NNP denotes Care
T9921 27143-27146 CC denotes and
T9922 27147-27150 NNP denotes Use
T9923 27151-27160 NNP denotes Committee
T10135 27163-27173 NN denotes Constructs
T10136 27174-27177 CC denotes and
T10137 27178-27190 NN denotes transfection
T10138 27191-27194 DT denotes The
T10139 27195-27208 JJ denotes complementary
T10140 27209-27212 NN denotes DNA
T10141 27213-27214 -LRB- denotes (
T10142 27214-27218 NN denotes cDNA
T10143 27218-27219 -RRB- denotes )
T10144 27220-27222 IN denotes of
T10145 27223-27226 DT denotes the
T10146 27227-27232 NN denotes mouse
T10147 27233-27240 NN denotes Sharpin
T10148 27241-27245 NN denotes gene
T10149 27246-27249 VB denotes was
T10150 27250-27256 VB denotes cloned
T10151 27257-27260 CC denotes and
T10152 27261-27270 VB denotes amplified
T10153 27271-27275 IN denotes from
T10154 27276-27284 NN denotes RAW264.7
T10155 27285-27288 NN denotes RNA
T10156 27289-27297 NN denotes extracts
T10157 27299-27302 DT denotes The
T10158 27303-27309 NN denotes primer
T10159 27310-27319 NN denotes sequences
T10160 27320-27324 VB denotes were
T10161 27325-27332 RB denotes forward
T10162 27332-27333 -COMMA- denotes ,
T10163 27334-27339 NN denotes 5′-CC
T10164 27340-27343 NN denotes ATG
T10165 27344-27347 NN denotes GCG
T10166 27348-27351 NN denotes ATG
T10167 27352-27355 NN denotes TCG
T10168 27356-27359 NN denotes CCG
T10169 27360-27363 NN denotes CCC
T10170 27364-27367 NN denotes GCC
T10171 27368-27371 NN denotes GGC
T10172 27372-27375 NN denotes GGT
T10173 27375-27376 -COLON- denotes ;
T10174 27377-27384 NN denotes reverse
T10175 27384-27385 -COMMA- denotes ,
T10176 27386-27389 JJ denotes 5′-
T10177 27390-27393 NN denotes AAG
T10178 27394-27397 NN denotes CTT
T10179 27398-27401 NN denotes CTA
T10180 27402-27405 NNP denotes GGT
T10181 27406-27409 NNP denotes GGA
T10182 27410-27413 NNP denotes AGC
T10183 27414-27417 NNP denotes TGC
T10184 27418-27421 NNP denotes AGC
T10185 27422-27425 NN denotes AAG
T10186 27426-27428 NN denotes A.
T10187 27429-27432 DT denotes The
T10188 27433-27440 NNP denotes Sharpin
T10189 27441-27445 NN denotes cDNA
T10190 27446-27449 VB denotes was
T10191 27450-27456 VB denotes cloned
T10192 27457-27461 IN denotes into
T10193 27462-27472 NN denotes expression
T10194 27473-27479 NN denotes vector
T10195 27480-27491 NN denotes pFLAG-CMV-2
T10196 27493-27499 JJ denotes Murine
T10197 27500-27511 NN denotes fibroblasts
T10198 27512-27515 CC denotes and
T10199 27516-27527 NN denotes macrophages
T10200 27528-27532 VB denotes were
T10201 27533-27537 VB denotes were
T10202 27538-27542 VB denotes used
T10203 27543-27545 TO denotes to
T10204 27546-27553 VB denotes express
T10205 27554-27565 JJ denotes recombinant
T10206 27566-27573 NN denotes SHARPIN
T10207 27574-27581 NN denotes protein
T10208 27583-27588 NN denotes Cells
T10209 27589-27590 -LRB- denotes (
T10210 27590-27595 CD denotes 2×104
T10211 27595-27596 -RRB- denotes )
T10212 27597-27601 VB denotes were
T10213 27602-27608 VB denotes seeded
T10214 27609-27611 IN denotes in
T10215 27612-27619 JJ denotes 96-well
T10216 27620-27627 VB denotes treated
T10217 27628-27634 VB denotes plates
T10218 27635-27638 DT denotes the
T10219 27639-27642 NN denotes day
T10220 27643-27649 IN denotes before
T10221 27650-27662 NN denotes transfection
T10222 27664-27669 IN denotes After
T10223 27670-27679 JJ denotes overnight
T10224 27680-27690 NN denotes incubation
T10225 27690-27691 -COMMA- denotes ,
T10226 27692-27695 CD denotes 200
T10227 27696-27698 NN denotes ng
T10228 27699-27712 NN denotes pFLAG-SHARPIN
T10229 27713-27721 NN denotes plasmids
T10230 27722-27726 VB denotes were
T10231 27727-27738 VB denotes transfected
T10232 27739-27743 IN denotes with
T10233 27744-27747 CD denotes 0.5
T10234 27748-27750 NN denotes ul
T10235 27751-27764 NN denotes Lipofectamine
T10236 27765-27769 CD denotes 2000
T10237 27771-27773 CD denotes 24
T10238 27774-27779 NN denotes hours
T10239 27780-27785 RB denotes later
T10240 27785-27786 -COMMA- denotes ,
T10241 27787-27792 NN denotes cells
T10242 27793-27797 VB denotes were
T10243 27798-27807 VB denotes incubated
T10244 27808-27812 IN denotes with
T10245 27813-27818 JJ denotes fresh
T10246 27819-27826 NN denotes culture
T10247 27827-27833 NN denotes medium
T10248 27835-27840 IN denotes After
T10249 27841-27848 DT denotes another
T10250 27849-27851 CD denotes 24
T10251 27852-27857 NN denotes hours
T10252 27857-27858 -COMMA- denotes ,
T10253 27859-27864 NN denotes cells
T10254 27865-27869 VB denotes were
T10255 27870-27875 VB denotes lysed
T10256 27876-27878 TO denotes to
T10257 27879-27886 VB denotes confirm
T10258 27887-27890 DT denotes the
T10259 27891-27903 NN denotes FLAG-SHARPIN
T10260 27904-27914 NN denotes expression
T10261 27915-27917 IN denotes by
T10262 27918-27929 NN denotes immunoblots
T10263 27930-27934 IN denotes with
T10264 27935-27944 NN denotes anti-FLAG
T10265 27946-27951 NN denotes Cells
T10266 27952-27956 IN denotes with
T10267 27957-27959 DT denotes no
T10268 27960-27972 NN denotes transfection
T10269 27973-27976 CC denotes and
T10270 27977-27988 VB denotes transfected
T10271 27989-27993 IN denotes with
T10272 27994-27999 JJ denotes empty
T10273 28000-28006 NN denotes vector
T10274 28007-28018 NN denotes pFLAG-CMV-2
T10275 28019-28023 VB denotes were
T10276 28024-28028 VB denotes used
T10277 28029-28031 IN denotes as
T10278 28032-28040 JJ denotes negative
T10279 28041-28048 NN denotes control
T10280 28049-28051 IN denotes in
T10281 28052-28055 DT denotes all
T10282 28056-28068 NN denotes transfection
T10283 28069-28080 NN denotes experiments
T10381 28083-28093 NN denotes Generation
T10382 28094-28096 IN denotes of
T10383 28097-28101 NN denotes BMDC
T10384 28102-28106 NN denotes BMDC
T10385 28107-28111 VB denotes were
T10386 28112-28121 VB denotes developed
T10387 28122-28124 IN denotes as
T10388 28125-28135 RB denotes previously
T10389 28136-28144 VB denotes reported
T10390 28145-28146 -LRB- denotes [
T10391 28146-28148 CD denotes 54
T10392 28148-28149 -RRB- denotes ]
T10393 28149-28150 -COMMA- denotes ,
T10394 28151-28152 -LRB- denotes [
T10395 28152-28154 CD denotes 55
T10396 28154-28155 -RRB- denotes ]
T10397 28157-28162 NN denotes Cells
T10398 28163-28167 VB denotes were
T10399 28168-28177 VB denotes collected
T10400 28178-28183 IN denotes after
T10401 28184-28189 CD denotes 10–12
T10402 28190-28194 NN denotes days
T10403 28195-28197 IN denotes of
T10404 28198-28205 NN denotes culture
T10405 28207-28210 DT denotes The
T10406 28211-28215 NN denotes cell
T10407 28216-28221 NN denotes yield
T10408 28222-28225 VB denotes was
T10409 28226-28233 CD denotes 2–3×107
T10410 28234-28245 NN denotes cells/mouse
T10411 28246-28250 IN denotes with
T10412 28251-28256 CD denotes 80–95
T10413 28256-28257 NN denotes %
T10414 28258-28262 NN denotes BMDC
T10676 28265-28274 NN denotes Phenotype
T10677 28275-28277 IN denotes of
T10678 28278-28282 NN denotes BMDC
T10679 28283-28286 CC denotes and
T10680 28287-28294 JJ denotes splenic
T10681 28295-28297 NN denotes DC
T10682 28298-28305 NN denotes subsets
T10683 28306-28309 DT denotes The
T10684 28310-28319 NN denotes phenotype
T10685 28320-28322 IN denotes of
T10686 28323-28327 NN denotes BMDC
T10687 28328-28331 VB denotes was
T10688 28332-28342 VB denotes determined
T10689 28343-28349 IN denotes before
T10690 28350-28353 CC denotes and
T10691 28354-28359 IN denotes after
T10692 28360-28362 CD denotes 24
T10693 28363-28367 NN denotes hour
T10694 28368-28375 NN denotes culture
T10695 28376-28378 IN denotes in
T10696 28379-28382 DT denotes the
T10697 28383-28391 NN denotes presence
T10698 28392-28394 IN denotes of
T10699 28395-28398 CD denotes 100
T10700 28399-28404 NN denotes ng/mL
T10701 28405-28408 NN denotes LPS
T10702 28410-28413 DT denotes The
T10703 28414-28419 NN denotes cells
T10704 28420-28424 VB denotes were
T10705 28425-28434 VB denotes incubated
T10706 28435-28437 IN denotes in
T10707 28438-28441 NNP denotes PBS
T10708 28442-28446 IN denotes with
T10709 28447-28450 CD denotes 0.1
T10710 28450-28451 NN denotes %
T10711 28452-28456 NN denotes NaN3
T10712 28456-28457 -COMMA- denotes ,
T10713 28458-28459 CD denotes 1
T10714 28459-28460 NN denotes %
T10715 28461-28464 NN denotes BSA
T10716 28465-28468 CC denotes and
T10717 28469-28471 CD denotes 10
T10718 28471-28472 NN denotes %
T10719 28473-28479 JJ denotes normal
T10720 28480-28486 NN denotes rabbit
T10721 28487-28492 NN denotes serum
T10722 28493-28496 IN denotes for
T10723 28497-28499 CD denotes 20
T10724 28500-28507 NN denotes minutes
T10725 28508-28510 IN denotes on
T10726 28511-28514 NN denotes ice
T10727 28514-28515 -COMMA- denotes ,
T10728 28516-28522 VB denotes washed
T10729 28523-28526 CC denotes and
T10730 28527-28536 VB denotes incubated
T10731 28537-28540 IN denotes for
T10732 28541-28543 CD denotes 30
T10733 28544-28551 NN denotes minutes
T10734 28552-28554 IN denotes on
T10735 28555-28558 NN denotes ice
T10736 28559-28563 IN denotes with
T10737 28564-28568 NNP denotes Alex
T10738 28569-28582 JJ denotes Fluor-labeled
T10739 28583-28593 NN denotes anti-CD11c
T10740 28594-28595 -LRB- denotes (
T10741 28595-28603 NN denotes MCD11C20
T10742 28603-28604 -COMMA- denotes ,
T10743 28605-28611 NN denotes CALTAG
T10744 28611-28612 -RRB- denotes )
T10745 28613-28615 IN denotes in
T10746 28616-28627 NN denotes combination
T10747 28628-28632 IN denotes with
T10748 28633-28636 NN denotes PE-
T10749 28637-28646 NN denotes anti-CD40
T10750 28647-28648 -LRB- denotes (
T10751 28648-28652 CD denotes 3/23
T10752 28652-28653 -COMMA- denotes ,
T10753 28654-28656 NNP denotes BD
T10754 28657-28668 NNP denotes Biosciences
T10755 28668-28669 -RRB- denotes )
T10756 28669-28670 -COMMA- denotes ,
T10757 28671-28683 NN denotes PE-anti-CD80
T10758 28684-28685 -LRB- denotes (
T10759 28685-28692 NN denotes 16-10A1
T10760 28692-28693 -COMMA- denotes ,
T10761 28694-28705 NN denotes eBioscience
T10762 28705-28706 -RRB- denotes )
T10763 28706-28707 -COMMA- denotes ,
T10764 28708-28720 NN denotes PE-anti-CD86
T10765 28721-28722 -LRB- denotes (
T10766 28722-28727 NN denotes P03.1
T10767 28727-28728 -COMMA- denotes ,
T10768 28729-28740 NN denotes eBioscience
T10769 28740-28741 -RRB- denotes )
T10770 28741-28742 -COMMA- denotes ,
T10771 28743-28755 NN denotes PE-anti-CD14
T10772 28756-28757 -LRB- denotes (
T10773 28757-28762 NN denotes Sa2.8
T10774 28762-28763 -COMMA- denotes ,
T10775 28764-28775 NN denotes eBioscience
T10776 28775-28776 -RRB- denotes )
T10777 28777-28780 CC denotes and
T10778 28781-28793 VB denotes biotinylated
T10779 28794-28803 NN denotes anti-TLR4
T10780 28804-28805 -LRB- denotes (
T10781 28805-28814 NN denotes BioLegend
T10782 28814-28815 -RRB- denotes )
T10783 28817-28820 DT denotes The
T10784 28821-28826 NN denotes cells
T10785 28827-28836 VB denotes incubated
T10786 28837-28841 IN denotes with
T10787 28842-28854 VB denotes biotinylated
T10788 28855-28869 NN denotes anti-TLR4/MD-2
T10789 28870-28874 VB denotes were
T10790 28875-28881 VB denotes washed
T10791 28882-28887 RB denotes twice
T10792 28888-28891 CC denotes and
T10793 28892-28901 VB denotes incubated
T10794 28902-28905 IN denotes for
T10795 28906-28908 CD denotes 30
T10796 28909-28916 NN denotes minutes
T10797 28917-28919 IN denotes on
T10798 28920-28923 NN denotes ice
T10799 28924-28928 IN denotes with
T10800 28929-28938 NN denotes avidin-PE
T10801 28940-28942 TO denotes To
T10802 28943-28950 VB denotes isolate
T10803 28951-28958 JJ denotes splenic
T10804 28959-28961 NN denotes DC
T10805 28961-28962 -COMMA- denotes ,
T10806 28963-28970 NN denotes spleens
T10807 28971-28975 VB denotes were
T10808 28976-28987 NN denotes collagenase
T10809 28988-28996 VB denotes digested
T10810 28997-29000 CC denotes and
T10811 29001-29008 JJ denotes subject
T10812 29009-29011 TO denotes to
T10813 29012-29019 VB denotes Percoll
T10814 29020-29028 NN denotes gradient
T10815 29029-29043 NN denotes centrifugation
T10816 29045-29048 DT denotes The
T10817 29049-29054 NN denotes bands
T10818 29055-29057 IN denotes at
T10819 29058-29061 DT denotes the
T10820 29062-29067 CD denotes 35–55
T10821 29067-29068 NN denotes %
T10822 29069-29078 NN denotes interface
T10823 29079-29083 VB denotes were
T10824 29084-29093 VB denotes collected
T10825 29094-29096 TO denotes to
T10826 29097-29102 VB denotes stain
T10827 29103-29107 IN denotes with
T10828 29108-29121 NN denotes PE-anti-CD11c
T10829 29122-29123 -LRB- denotes (
T10830 29123-29126 NN denotes HL3
T10831 29126-29127 -COMMA- denotes ,
T10832 29128-29137 NNP denotes BioLegend
T10833 29137-29138 -RRB- denotes )
T10834 29138-29139 -COMMA- denotes ,
T10835 29140-29155 NN denotes APC-anti-PDCA-1
T10836 29156-29157 -LRB- denotes (
T10837 29157-29160 CD denotes 927
T10838 29160-29161 -COMMA- denotes ,
T10839 29162-29171 NNP denotes BioLegend
T10840 29171-29172 -RRB- denotes )
T10841 29173-29176 CC denotes and
T10842 29177-29191 NN denotes FITC-anti-CD8α
T10843 29192-29193 -LRB- denotes (
T10844 29193-29199 CD denotes 53–6.7
T10845 29199-29200 -COMMA- denotes ,
T10846 29201-29203 NNP denotes BD
T10847 29204-29214 NNP denotes BioScience
T10848 29214-29215 -RRB- denotes )
T10849 29217-29220 DT denotes The
T10850 29221-29226 NN denotes cells
T10851 29227-29231 VB denotes were
T10852 29232-29238 VB denotes washed
T10853 29239-29244 RB denotes twice
T10854 29244-29245 -COMMA- denotes ,
T10855 29246-29251 VB denotes fixed
T10856 29252-29254 IN denotes in
T10857 29255-29256 CD denotes 2
T10858 29256-29257 NN denotes %
T10859 29258-29274 NN denotes paraformaldehyde
T10860 29274-29275 -COMMA- denotes ,
T10861 29276-29279 CC denotes and
T10862 29280-29286 VB denotes stored
T10863 29287-29289 IN denotes at
T10864 29290-29293 NN denotes 4°C
T10865 29294-29299 IN denotes until
T10866 29300-29308 NN denotes analysis
T10867 29310-29314 NN denotes Flow
T10868 29315-29324 NN denotes cytometry
T10869 29325-29328 VB denotes was
T10870 29329-29338 VB denotes performed
T10871 29339-29341 IN denotes in
T10872 29342-29344 DT denotes an
T10873 29345-29350 NNP denotes Excel
T10874 29351-29352 -LRB- denotes (
T10875 29352-29359 NNP denotes Coulter
T10876 29359-29360 -RRB- denotes )
T10877 29361-29371 NN denotes instrument
T10878 29373-29377 NN denotes Dead
T10879 29378-29383 NN denotes cells
T10880 29384-29388 VB denotes were
T10881 29389-29396 VB denotes omitted
T10882 29397-29401 IN denotes from
T10883 29402-29405 DT denotes the
T10884 29406-29414 NN denotes analysis
T10885 29415-29417 IN denotes by
T10886 29418-29424 VB denotes gating
T10887 29425-29427 IN denotes on
T10888 29428-29435 JJ denotes forward
T10889 29436-29439 CC denotes and
T10890 29440-29443 CD denotes 90°
T10891 29444-29449 JJ denotes light
T10892 29450-29457 NN denotes scatter
T10893 29457-29458 -COMMA- denotes ,
T10894 29459-29462 CC denotes and
T10895 29463-29469 CD denotes 10,000
T10896 29470-29475 NN denotes cells
T10897 29476-29480 VB denotes were
T10898 29481-29489 VB denotes analyzed
T10899 29490-29492 IN denotes by
T10900 29493-29499 NNP denotes FlowJo
T10901 29500-29508 NN denotes software
T11111 29511-29517 NN denotes BMDC-T
T11112 29518-29522 NN denotes cell
T11113 29523-29525 FW denotes in
T11114 29526-29531 FW denotes vitro
T11115 29532-29543 NN denotes interaction
T11116 29544-29546 NN denotes WT
T11117 29547-29550 CC denotes and
T11118 29551-29555 NN denotes cpdm
T11119 29556-29560 NN denotes BMDC
T11120 29561-29562 -LRB- denotes (
T11121 29562-29567 CD denotes 5×104
T11122 29567-29568 -RRB- denotes )
T11123 29569-29573 VB denotes were
T11124 29574-29584 VB denotes stimulated
T11125 29585-29589 IN denotes with
T11126 29590-29596 NN denotes medium
T11127 29596-29597 -COMMA- denotes ,
T11128 29598-29599 CD denotes 1
T11129 29600-29605 NN denotes µg/mL
T11130 29606-29609 NN denotes LPS
T11131 29609-29610 -COMMA- denotes ,
T11132 29611-29613 CD denotes 25
T11133 29614-29619 NN denotes µg/mL
T11134 29620-29623 CC denotes and
T11135 29624-29625 CD denotes 5
T11136 29626-29631 NN denotes µg/mL
T11137 29632-29639 NN denotes Pam3CYS
T11138 29641-29643 CD denotes 24
T11139 29644-29649 NN denotes hours
T11140 29650-29655 RB denotes later
T11141 29655-29656 -COMMA- denotes ,
T11142 29657-29662 NN denotes cells
T11143 29663-29667 VB denotes were
T11144 29668-29677 VB denotes collected
T11145 29678-29681 CC denotes and
T11146 29682-29688 VB denotes washed
T11147 29689-29693 IN denotes with
T11148 29694-29697 NNP denotes PBS
T11149 29699-29709 JJ denotes Allogeneic
T11150 29710-29715 NN denotes naïve
T11151 29716-29720 JJ denotes CD4+
T11152 29721-29722 NN denotes T
T11153 29723-29728 NN denotes cells
T11154 29729-29733 VB denotes were
T11155 29734-29742 VB denotes isolated
T11156 29743-29747 IN denotes from
T11157 29748-29755 NN denotes spleens
T11158 29756-29758 IN denotes of
T11159 29759-29765 NN denotes BALB/c
T11160 29766-29770 NN denotes mice
T11161 29771-29773 IN denotes by
T11162 29774-29782 JJ denotes negative
T11163 29783-29792 NN denotes selection
T11164 29793-29796 NN denotes kit
T11165 29797-29798 -LRB- denotes (
T11166 29798-29808 NN denotes Invitrogen
T11167 29808-29809 -RRB- denotes )
T11168 29810-29813 CC denotes and
T11169 29814-29818 VB denotes were
T11170 29819-29823 RB denotes then
T11171 29824-29829 VB denotes added
T11172 29830-29832 IN denotes at
T11173 29833-29840 CD denotes 2.5×105
T11174 29841-29844 CC denotes and
T11175 29845-29856 VB denotes co-cultured
T11176 29857-29861 IN denotes with
T11177 29862-29871 VB denotes activated
T11178 29872-29876 NN denotes BMDC
T11179 29878-29883 IN denotes After
T11180 29884-29885 CD denotes 5
T11181 29886-29890 NN denotes days
T11182 29890-29891 -COMMA- denotes ,
T11183 29892-29903 NN denotes supernatant
T11184 29904-29907 VB denotes was
T11185 29908-29917 VB denotes collected
T11186 29918-29921 CC denotes and
T11187 29922-29925 DT denotes the
T11188 29926-29935 NN denotes secretion
T11189 29936-29938 IN denotes of
T11190 29939-29943 NN denotes IFNγ
T11191 29943-29944 -COMMA- denotes ,
T11192 29945-29948 NN denotes IL4
T11193 29948-29949 -COMMA- denotes ,
T11194 29950-29953 NN denotes IL2
T11195 29953-29954 -COMMA- denotes ,
T11196 29955-29958 CC denotes and
T11197 29959-29964 NN denotes IL17A
T11198 29965-29973 VB denotes measured
T11199 29974-29976 IN denotes by
T11200 29977-29982 NN denotes ELISA
T11201 29984-29992 JJ denotes Negative
T11202 29993-30001 NN denotes controls
T11203 30002-30005 VB denotes are
T11204 30006-30007 CD denotes 1
T11205 30007-30008 -RRB- denotes )
T11206 30009-30019 VB denotes stimulated
T11207 30020-30024 NN denotes BMDC
T11208 30025-30032 IN denotes without
T11209 30033-30043 NN denotes co-culture
T11210 30044-30048 IN denotes with
T11211 30049-30059 JJ denotes allogeneic
T11212 30060-30064 JJ denotes CD4+
T11213 30065-30066 NN denotes T
T11214 30067-30072 NN denotes cells
T11215 30072-30073 -COLON- denotes ;
T11216 30074-30075 LS denotes 2
T11217 30075-30076 -RRB- denotes )
T11218 30077-30087 JJ denotes allogeneic
T11219 30088-30092 JJ denotes CD4+
T11220 30093-30094 NN denotes T
T11221 30095-30100 NN denotes cells
T11222 30101-30108 IN denotes without
T11223 30109-30119 NN denotes co-culture
T11224 30120-30124 IN denotes with
T11225 30125-30135 VB denotes stimulated
T11226 30136-30140 NN denotes BMDC
T11227 30142-30146 DT denotes Both
T11228 30147-30155 JJ denotes negative
T11229 30156-30164 NN denotes controls
T11230 30165-30169 VB denotes show
T11231 30170-30172 DT denotes no
T11232 30173-30183 NN denotes production
T11233 30184-30186 IN denotes of
T11234 30187-30201 JJ denotes aforementioned
T11235 30202-30211 NN denotes cytokines
T11602 30214-30217 NN denotes RNA
T11603 30218-30228 NN denotes expression
T11604 30229-30231 IN denotes by
T11605 30232-30236 NN denotes BMDC
T11606 30237-30241 NN denotes BMDC
T11607 30242-30246 VB denotes were
T11608 30247-30255 VB denotes cultured
T11609 30256-30258 IN denotes at
T11610 30259-30262 CD denotes 106
T11611 30263-30271 NN denotes cells/mL
T11612 30272-30274 IN denotes in
T11613 30275-30277 CD denotes 10
T11614 30278-30280 NN denotes mL
T11615 30281-30283 IN denotes of
T11616 30284-30293 NN denotes RPMI-1640
T11617 30294-30302 JJ denotes complete
T11618 30303-30309 NN denotes medium
T11619 30310-30312 IN denotes in
T11620 30313-30316 DT denotes the
T11621 30317-30325 NN denotes presence
T11622 30326-30328 CC denotes or
T11623 30329-30336 NN denotes absence
T11624 30337-30339 IN denotes of
T11625 30340-30343 CD denotes 100
T11626 30344-30349 NN denotes ng/mL
T11627 30350-30353 NN denotes LPS
T11628 30354-30356 CC denotes or
T11629 30357-30359 CD denotes 25
T11630 30360-30365 NN denotes µg/mL
T11631 30366-30370 NN denotes poly
T11632 30371-30372 CD denotes I
T11633 30372-30373 -COLON- denotes :
T11634 30373-30375 NNP denotes C.
T11635 30376-30381 IN denotes After
T11636 30382-30383 CD denotes 1
T11637 30384-30387 CC denotes and
T11638 30388-30389 CD denotes 2
T11639 30390-30395 NN denotes hours
T11640 30395-30396 -COMMA- denotes ,
T11641 30397-30400 NN denotes RNA
T11642 30401-30404 VB denotes was
T11643 30405-30413 VB denotes isolated
T11644 30414-30418 IN denotes with
T11645 30419-30430 NN denotes TRI-reagent
T11646 30431-30432 -LRB- denotes (
T11647 30432-30437 NN denotes Sigma
T11648 30437-30438 -RRB- denotes )
T11649 30439-30448 VB denotes according
T11650 30449-30451 TO denotes to
T11651 30452-30455 DT denotes the
T11652 30456-30468 NN denotes manufacturer
T11653 30468-30470 POS denotes 's
T11654 30471-30483 NN denotes instructions
T11655 30485-30488 DT denotes The
T11656 30489-30499 NN denotes expression
T11657 30500-30502 IN denotes of
T11658 30503-30506 NN denotes Il6
T11659 30506-30507 -COMMA- denotes ,
T11660 30508-30515 NN denotes Il12p40
T11661 30515-30516 -COMMA- denotes ,
T11662 30517-30522 NN denotes Gmcsf
T11663 30522-30523 -COMMA- denotes ,
T11664 30524-30527 CC denotes and
T11665 30528-30535 NN denotes Sharpin
T11666 30536-30540 NN denotes mRNA
T11667 30541-30544 VB denotes was
T11668 30545-30555 VB denotes determined
T11669 30556-30558 IN denotes by
T11670 30559-30566 NN denotes qRT-PCR
T11671 30568-30575 NN denotes Primers
T11672 30576-30579 CC denotes and
T11673 30580-30586 NN denotes probes
T11674 30587-30591 VB denotes were
T11675 30592-30601 VB denotes purchased
T11676 30602-30606 IN denotes from
T11677 30607-30614 NNP denotes Applied
T11678 30615-30626 NNP denotes Biosystems.
T11679 30627-30634 NNP denotes Reverse
T11680 30635-30648 NN denotes transcription
T11681 30649-30652 VB denotes was
T11682 30653-30662 VB denotes performed
T11683 30663-30665 IN denotes at
T11684 30666-30670 NN denotes 42°C
T11685 30671-30674 IN denotes for
T11686 30675-30677 CD denotes 60
T11687 30678-30685 NN denotes minutes
T11688 30686-30690 IN denotes with
T11689 30691-30694 DT denotes the
T11690 30695-30700 JJ denotes final
T11691 30701-30713 NN denotes denaturation
T11692 30714-30718 NN denotes step
T11693 30719-30721 IN denotes at
T11694 30722-30726 NN denotes 90°C
T11695 30727-30730 IN denotes for
T11696 30731-30732 CD denotes 5
T11697 30733-30740 NN denotes minutes
T11698 30741-30743 IN denotes in
T11699 30744-30746 CD denotes 30
T11700 30747-30749 NN denotes µl
T11701 30750-30760 VB denotes containing
T11702 30761-30764 CD denotes 0.5
T11703 30765-30767 NN denotes µg
T11704 30768-30770 IN denotes of
T11705 30771-30776 JJ denotes total
T11706 30777-30781 NN denotes RNA.
T11707 30782-30787 NN denotes dNTPs
T11708 30787-30788 -COMMA- denotes ,
T11709 30789-30794 NN denotes oligo
T11710 30794-30795 -LRB- denotes (
T11711 30795-30797 NN denotes dT
T11712 30797-30798 -RRB- denotes )
T11713 30798-30800 CD denotes 15
T11714 30801-30807 NN denotes primer
T11715 30807-30808 -COMMA- denotes ,
T11716 30809-30820 JJ denotes recombinant
T11717 30821-30827 NNP denotes RNasin
T11718 30828-30840 NNP denotes Ribonuclease
T11719 30841-30850 NNP denotes Inhibitor
T11720 30850-30851 -COMMA- denotes ,
T11721 30852-30855 CC denotes and
T11722 30856-30861 NN denotes M-MLV
T11723 30862-30869 JJ denotes Reverse
T11724 30870-30883 NN denotes Transcriptase
T11725 30884-30885 -LRB- denotes (
T11726 30885-30888 DT denotes all
T11727 30889-30893 IN denotes from
T11728 30894-30901 NNP denotes Promega
T11729 30901-30902 -COMMA- denotes ,
T11730 30903-30910 NNP denotes Madison
T11731 30910-30911 -COMMA- denotes ,
T11732 30912-30914 NNP denotes WI
T11733 30914-30915 -RRB- denotes )
T11734 30916-30920 VB denotes were
T11735 30921-30925 VB denotes used
T11736 30926-30935 VB denotes according
T11737 30936-30938 TO denotes to
T11738 30939-30942 DT denotes the
T11739 30943-30955 NN denotes manufacturer
T11740 30955-30957 POS denotes 's
T11741 30958-30969 NN denotes instruction
T11742 30971-30978 JJ denotes Reverse
T11743 30979-30992 NN denotes transcription
T11744 30993-30996 VB denotes was
T11745 30997-31001 VB denotes done
T11746 31002-31004 IN denotes in
T11747 31005-31006 DT denotes a
T11748 31007-31014 NN denotes PTC-200
T11749 31015-31022 NNP denotes Peltier
T11750 31023-31030 NNP denotes Thermal
T11751 31031-31037 NNP denotes Cycler
T11752 31038-31039 -LRB- denotes (
T11753 31039-31041 NNP denotes MJ
T11754 31042-31050 NNP denotes Research
T11755 31050-31051 -COMMA- denotes ,
T11756 31052-31061 NNP denotes Watertown
T11757 31061-31062 -COMMA- denotes ,
T11758 31063-31065 JJ denotes MA
T11759 31065-31066 -RRB- denotes )
T11760 31066-31073 NN denotes . qRT-P
T11761 31073-31076 VB denotes CR
T11762 31076-31085 VB denotes was perfo
T11763 31085-31087 IN denotes rm
T11764 31087-31090 NNP denotes ed
T11765 31090-31095 NNP denotes in AB
T11766 31095-31099 CD denotes I Pr
T11767 31099-31107 NNP denotes ism 7700
T11768 31108-31117 NNP denotes Sequence
T11769 31117-31123 NNP denotes Detect
T11770 31123-31127 IN denotes ion
T11771 31127-31134 NNP denotes System
T11772 31134-31138 NNP denotes with
T11773 31139-31149 NN denotes TaqMan® Ge
T11774 31149-31155 NN denotes ne Exp
T11775 31155-31156 -LRB- denotes r
T11776 31156-31163 NNP denotes ession
T11777 31163-31173 NNP denotes Assays (Ap
T11778 31173-31174 -COMMA- denotes p
T11779 31174-31180 NNP denotes lied B
T11780 31180-31184 NNP denotes iosy
T11781 31184-31185 -COMMA- denotes s
T11782 31185-31187 NNP denotes te
T11783 31187-31188 -RRB- denotes m
T11784 31188-31191 IN denotes s,
T11785 31191-31196 NN denotes Foste
T11786 31196-31200 NN denotes r Ci
T11787 31200-31201 -COMMA- denotes t
T11788 31201-31204 NN denotes y,
T11789 31204-31205 -COMMA- denotes C
T11790 31205-31212 NN denotes A) for
T11791 31212-31213 -COMMA- denotes m
T11792 31213-31217 NNP denotes ouse
T11793 31218-31219 -COMMA- denotes A
T11794 31219-31224 NNP denotes ctb,
T11795 31224-31225 -COMMA- denotes I
T11796 31225-31228 CC denotes L6,
T11797 31229-31236 NNP denotes IL12p40
T11798 31236-31245 VB denotes , Ifnβ, G
T11799 31245-31247 TO denotes mc
T11800 31247-31250 DT denotes sf,
T11801 31251-31263 NN denotes and Sharpin
T11802 31263-31265 POS denotes ac
T11803 31265-31273 NN denotes cording
T11804 31305-31308 DT denotes The
T11805 31309-31319 JJ denotes endogenous
T11806 31320-31328 NN denotes standard
T11807 31329-31332 IN denotes for
T11808 31333-31346 NN denotes normalization
T11809 31347-31349 IN denotes of
T11810 31350-31353 DT denotes the
T11811 31354-31360 NN denotes target
T11812 31361-31365 NN denotes gene
T11813 31366-31369 VB denotes was
T11814 31370-31377 JJ denotes β-actin
T11815 31379-31387 JJ denotes Relative
T11816 31388-31392 NN denotes gene
T11817 31393-31403 NN denotes expression
T11818 31404-31407 VB denotes was
T11819 31408-31418 VB denotes calculated
T11820 31419-31424 VB denotes using
T11821 31425-31428 DT denotes the
T11822 31429-31435 NN denotes 2−ΔΔCt
T11823 31436-31442 NN denotes method
T11824 31443-31444 -LRB- denotes [
T11825 31444-31446 CD denotes 56
T11826 31446-31447 -RRB- denotes ]
T12001 31450-31452 FW denotes In
T12002 31453-31458 FW denotes vitro
T12003 31459-31467 NN denotes cytokine
T12004 31468-31477 NN denotes secretion
T12005 31478-31480 IN denotes by
T12006 31481-31485 NN denotes BMDC
T12007 31486-31489 DT denotes The
T12008 31490-31494 NN denotes BMDC
T12009 31495-31499 VB denotes were
T12010 31500-31508 VB denotes cultured
T12011 31509-31511 IN denotes in
T12012 31512-31522 JJ denotes triplicate
T12013 31523-31528 NN denotes wells
T12014 31529-31531 IN denotes of
T12015 31532-31539 JJ denotes 24-well
T12016 31540-31542 CC denotes or
T12017 31543-31550 JJ denotes 96-well
T12018 31551-31557 NN denotes plates
T12023 31572-31580 NN denotes cells/mL
T12024 31580-31581 -COMMA- denotes ,
T12025 31582-31594 RB denotes respectively
T12026 31594-31595 -COMMA- denotes ,
T12027 31596-31598 IN denotes in
T12028 31599-31608 NN denotes RPMI-1640
T12029 31609-31617 JJ denotes complete
T12030 31618-31624 NN denotes medium
T12031 31626-31629 DT denotes The
T12032 31630-31635 NN denotes cells
T12033 31636-31640 VB denotes were
T12034 31641-31647 VB denotes washed
T12035 31648-31651 CC denotes and
T12036 31652-31662 VB denotes stimulated
T12037 31663-31667 IN denotes with
T12038 31668-31671 CD denotes 100
T12039 31672-31677 NN denotes ng/mL
T12040 31678-31681 NN denotes LPS
T12041 31682-31684 CC denotes or
T12042 31685-31687 CD denotes 25
T12043 31688-31693 NN denotes µg/mL
T12044 31694-31698 NN denotes poly
T12045 31699-31700 CD denotes I
T12046 31700-31701 -COLON- denotes :
T12047 31701-31703 NNP denotes C.
T12048 31704-31709 IN denotes After
T12049 31710-31712 CD denotes 24
T12050 31713-31718 NN denotes hours
T12051 31718-31719 -COMMA- denotes ,
T12052 31720-31732 NN denotes supernatants
T12053 31733-31737 VB denotes were
T12054 31738-31747 VB denotes harvested
T12055 31748-31751 IN denotes for
T12056 31752-31757 NN denotes ELISA
T12057 31758-31766 NN denotes analysis
T12058 31768-31771 DT denotes The
T12059 31772-31780 NN denotes presence
T12060 31781-31783 IN denotes of
T12061 31784-31791 NN denotes nitrite
T12062 31792-31794 IN denotes in
T12063 31795-31798 DT denotes the
T12064 31799-31811 NN denotes supernatants
T12065 31812-31815 VB denotes was
T12066 31816-31826 VB denotes determined
T12067 31827-31832 VB denotes using
T12068 31833-31836 DT denotes the
T12069 31837-31843 NNP denotes Griess
T12070 31844-31851 NN denotes reagent
T12242 32013-32021 NN denotes methanol
T12243 32022-32024 IN denotes at
T12244 32025-32030 NN denotes −20°C
T12245 32031-32034 IN denotes for
T12246 32035-32037 CD denotes 10
T12247 32038-32045 NN denotes minutes
T12248 32046-32049 CC denotes and
T12249 32050-32054 JJ denotes cold
T12250 32055-32062 NN denotes acetone
T12251 32063-32065 IN denotes at
T12252 32066-32071 NN denotes -20°C
T12253 32072-32075 IN denotes for
T12254 32076-32077 CD denotes 1
T12255 32078-32084 NN denotes minute
T12256 32086-32094 NN denotes Incubate
T12257 32095-32100 NN denotes cells
T12258 32101-32105 IN denotes with
T12259 32106-32111 NN denotes PBS+1
T12260 32111-32112 NN denotes %
T12261 32113-32116 NN denotes BSA
T12262 32117-32120 IN denotes for
T12263 32121-32123 CD denotes 15
T12264 32124-32131 NN denotes minutes
T12265 32132-32134 TO denotes to
T12266 32135-32140 VB denotes block
T12267 32141-32153 JJ denotes non-specific
T12268 32154-32161 NN denotes binding
T12269 32163-32168 VB denotes Fixed
T12270 32169-32174 NN denotes cells
T12271 32175-32179 VB denotes were
T12272 32180-32184 RB denotes then
T12273 32185-32194 VB denotes incubated
T12274 32195-32199 IN denotes with
T12275 32200-32209 JJ denotes anti-FLAG
T12276 32210-32211 -LRB- denotes (
T12277 32211-32216 CD denotes 1∶100
T12278 32216-32217 -RRB- denotes )
T12279 32218-32220 IN denotes as
T12280 32221-32224 DT denotes the
T12281 32225-32232 JJ denotes primary
T12282 32233-32241 NN denotes antibody
T12283 32242-32244 IN denotes at
T12284 32245-32249 NN denotes room
T12285 32250-32261 NN denotes temperature
T12286 32262-32265 IN denotes for
T12287 32266-32267 CD denotes 1
T12288 32268-32272 NN denotes hour
T12289 32272-32273 -COMMA- denotes ,
T12290 32274-32282 VB denotes followed
T12291 32283-32285 IN denotes by
T12292 32286-32291 CD denotes three
T12293 32292-32300 JJ denotes 5-minute
T12294 32301-32307 NN denotes washes
T12295 32309-32314 NN denotes Cells
T12296 32315-32319 VB denotes were
T12297 32320-32327 RB denotes further
T12298 32328-32337 VB denotes incubated
T12299 32338-32342 IN denotes with
T12300 32343-32347 NN denotes goat
T12301 32348-32359 JJ denotes anti-rabbit
T12302 32360-32368 NN denotes IgG-FITC
T12303 32369-32370 -LRB- denotes (
T12304 32370-32375 CD denotes 1∶100
T12305 32375-32376 -RRB- denotes )
T12306 32377-32379 IN denotes as
T12307 32380-32383 DT denotes the
T12308 32384-32393 JJ denotes secondary
T12309 32394-32402 NN denotes antibody
T12310 32403-32405 IN denotes at
T12311 32406-32410 NN denotes room
T12312 32411-32422 NN denotes temperature
T12313 32423-32426 IN denotes for
T12314 32427-32429 CD denotes 30
T12315 32430-32437 NN denotes minutes
T12316 32437-32438 -COMMA- denotes ,
T12317 32439-32447 VB denotes followed
T12318 32448-32450 IN denotes by
T12319 32451-32456 CD denotes three
T12320 32457-32465 JJ denotes 5-minute
T12321 32466-32472 NN denotes washes
T12322 32474-32479 NN denotes Cells
T12323 32480-32484 VB denotes were
T12324 32485-32489 RB denotes then
T12325 32490-32498 VB denotes examined
T12326 32499-32504 VB denotes using
T12327 32505-32512 JJ denotes inverse
T12328 32513-32525 NN denotes fluorescence
T12329 32526-32536 NN denotes microscope
T12503 32539-32550 NN denotes Immunoblots
T12504 32551-32556 IN denotes After
T12505 32557-32563 VB denotes adding
T12506 32564-32575 VB denotes stimulating
T12507 32576-32583 NN denotes ligands
T12508 32583-32584 -COMMA- denotes ,
T12509 32585-32588 CD denotes 100
T12510 32589-32594 NN denotes ng/mL
T12511 32595-32598 NN denotes LPS
T12512 32599-32601 CC denotes or
T12513 32602-32604 CD denotes 25
T12514 32605-32610 NN denotes µg/mL
T12515 32611-32615 NN denotes poly
T12516 32616-32617 NN denotes I
T12517 32617-32618 -COLON- denotes :
T12518 32618-32619 NN denotes C
T12519 32619-32620 -COMMA- denotes ,
T12520 32621-32625 NN denotes BMDC
T12521 32626-32630 VB denotes were
T12522 32631-32640 VB denotes collected
T12523 32641-32644 CC denotes and
T12524 32645-32650 VB denotes lysed
T12525 32651-32653 IN denotes at
T12526 32654-32656 CD denotes 0-
T12527 32656-32657 -COMMA- denotes ,
T12528 32658-32661 CD denotes 15-
T12529 32661-32662 -COMMA- denotes ,
T12530 32663-32666 CD denotes 30-
T12531 32666-32667 -COMMA- denotes ,
T12532 32668-32671 CC denotes and
T12533 32672-32681 JJ denotes 60-minute
T12534 32682-32686 NN denotes time
T12535 32687-32693 NN denotes points
T12536 32695-32706 NN denotes Immunoblots
T12537 32707-32711 VB denotes were
T12538 32712-32721 VB denotes performed
T12539 32722-32726 IN denotes with
T12540 32727-32737 NN denotes antibodies
T12541 32738-32739 -LRB- denotes (
T12542 32739-32743 NNP denotes Cell
T12543 32744-32753 NNP denotes Signaling
T12544 32754-32764 NNP denotes Technology
T12545 32764-32765 -RRB- denotes )
T12546 32766-32773 IN denotes against
T12547 32774-32782 NN denotes p-IKK1/2
T12548 32782-32783 -LRB- denotes (
T12549 32783-32784 -SHARP- denotes #
T12550 32784-32788 CD denotes 2697
T12551 32788-32789 -RRB- denotes )
T12552 32789-32790 -COMMA- denotes ,
T12553 32791-32797 NN denotes p-IκBα
T12554 32798-32799 -LRB- denotes (
T12555 32799-32800 -SHARP- denotes #
T12556 32800-32804 CD denotes 9246
T12557 32804-32805 -RRB- denotes )
T12558 32805-32806 -COMMA- denotes ,
T12559 32807-32811 NN denotes IκBα
T12560 32812-32813 -LRB- denotes (
T12561 32813-32814 -SHARP- denotes #
T12562 32814-32818 CD denotes 4814
T12563 32818-32819 -RRB- denotes )
T12564 32819-32820 -COMMA- denotes ,
T12565 32821-32827 NN denotes p-TBK1
T12566 32827-32828 -LRB- denotes (
T12567 32828-32829 -SHARP- denotes #
T12568 32829-32833 CD denotes 5483
T12569 32833-32834 -RRB- denotes )
T12570 32834-32835 -COMMA- denotes ,
T12571 32836-32841 NN denotes p-p38
T12572 32842-32843 -LRB- denotes (
T12573 32843-32844 -SHARP- denotes #
T12574 32844-32848 CD denotes 9216
T12575 32848-32849 -RRB- denotes )
T12576 32849-32850 -COMMA- denotes ,
T12577 32851-32854 NN denotes p38
T12578 32855-32856 -LRB- denotes (
T12579 32856-32857 -SHARP- denotes #
T12580 32857-32861 CD denotes 9212
T12581 32861-32862 -RRB- denotes )
T12582 32862-32863 -COMMA- denotes ,
T12583 32864-32872 NN denotes p-ERK1/2
T12584 32873-32874 -LRB- denotes (
T12585 32874-32875 -SHARP- denotes #
T12586 32875-32879 CD denotes 4376
T12587 32879-32880 -RRB- denotes )
T12588 32880-32881 -COMMA- denotes ,
T12589 32882-32885 CC denotes and
T12590 32886-32892 NN denotes ERK1/2
T12591 32893-32894 -LRB- denotes (
T12592 32894-32895 -SHARP- denotes #
T12593 32895-32899 CD denotes 4695
T12594 32899-32900 -RRB- denotes )
T12595 32902-32912 NN denotes Beta-actin
T12596 32913-32914 -LRB- denotes (
T12597 32914-32922 NN denotes sc-47778
T12598 32922-32923 -COMMA- denotes ,
T12599 32924-32929 NNP denotes Santa
T12600 32930-32934 NNP denotes Cruz
T12601 32935-32948 NNP denotes Biotechnology
T12602 32948-32949 -RRB- denotes )
T12603 32950-32953 VB denotes was
T12604 32954-32958 VB denotes used
T12605 32959-32961 IN denotes as
T12606 32962-32969 VB denotes loading
T12607 32970-32977 NN denotes control
T12709 32980-32991 JJ denotes Statistical
T12710 32992-33000 NN denotes analysis
T12711 33001-33005 NN denotes Data
T12712 33006-33009 VB denotes are
T12713 33010-33019 VB denotes expressed
T12714 33020-33022 IN denotes as
T12715 33023-33027 JJ denotes mean
T12716 33028-33029 NN denotes ±
T12717 33030-33032 NN denotes SD
T12718 33034-33037 DT denotes The
T12719 33038-33049 JJ denotes statistical
T12720 33050-33062 NN denotes significance
T12721 33063-33065 IN denotes of
T12722 33066-33077 NN denotes differences
T12723 33078-33080 IN denotes of
T12724 33081-33086 NN denotes means
T12725 33087-33094 IN denotes between
T12726 33095-33107 JJ denotes experimental
T12727 33108-33114 NN denotes groups
T12728 33115-33118 VB denotes was
T12729 33119-33129 VB denotes determined
T12730 33130-33132 IN denotes by
T12731 33133-33141 NN denotes Students
T12732 33141-33142 POS denotes '
T12733 33143-33149 NN denotes t-test
T12991 6096-6098 FW denotes In
T12992 6099-6103 FW denotes vivo
T12993 6104-6107 CC denotes and
T12994 6108-6110 FW denotes in
T12995 6111-6116 FW denotes vitro
T12996 6117-6125 NN denotes features
T12997 6126-6128 IN denotes of
T12998 6129-6136 NN denotes SHARPIN
T12999 6138-6139 -LRB- denotes (
T13000 6139-6140 NN denotes A
T13001 6140-6141 -RRB- denotes )
T13002 6142-6147 NN denotes COILS
T13003 6148-6151 CC denotes and
T13004 6152-6161 NNP denotes MotifScan
T13005 6162-6170 NN denotes programs
T13006 6171-6175 VB denotes were
T13007 6176-6180 VB denotes used
T13008 6181-6183 TO denotes to
T13009 6184-6191 VB denotes predict
T13010 6192-6195 DT denotes the
T13011 6196-6204 NN denotes presence
T13012 6205-6207 IN denotes of
T13013 6208-6210 NN denotes CC
T13014 6211-6212 -LRB- denotes (
T13015 6212-6223 NN denotes coiled-coil
T13016 6223-6224 -RRB- denotes )
T13017 6225-6231 NN denotes domain
T13018 6231-6232 -COMMA- denotes ,
T13019 6233-6236 NN denotes UBL
T13020 6237-6238 -LRB- denotes (
T13021 6238-6252 JJ denotes ubiquitin-like
T13022 6252-6253 -RRB- denotes )
T13023 6254-6260 NN denotes domain
T13024 6261-6264 CC denotes and
T13025 6265-6270 NN denotes ZFRBP
T13026 6271-6272 -LRB- denotes (
T13027 6272-6283 NN denotes zinc-finger
T13028 6284-6295 NN denotes Ran-Binding
T13029 6296-6303 NN denotes protein
T13030 6304-6305 CD denotes 2
T13031 6305-6306 -RRB- denotes )
T13032 6307-6313 NN denotes domain
T13033 6314-6319 WDT denotes which
T13034 6320-6324 VB denotes form
T13035 6325-6332 JJ denotes similar
T13036 6333-6338 NN denotes motif
T13037 6339-6347 NN denotes patterns
T13038 6348-6350 IN denotes in
T13039 6351-6354 DT denotes the
T13040 6355-6362 NN denotes SHARPIN
T13041 6363-6370 NN denotes protein
T13042 6371-6373 IN denotes of
T13043 6374-6379 JJ denotes human
T13044 6379-6380 -COMMA- denotes ,
T13045 6381-6386 NN denotes mouse
T13046 6387-6390 CC denotes and
T13047 6391-6394 NN denotes rat
T13048 6395-6403 NN denotes origins.
T13049 6404-6405 -LRB- denotes (
T13050 6405-6406 NN denotes B
T13051 6406-6407 -RRB- denotes )
T13052 6408-6413 CD denotes Eight
T13053 6414-6422 JJ denotes week-old
T13054 6423-6430 NN denotes females
T13055 6431-6433 IN denotes of
T13056 6434-6436 NN denotes WT
T13057 6437-6440 CC denotes and
T13058 6441-6445 NN denotes cpdm
T13059 6446-6450 NN denotes mice
T13060 6452-6455 DT denotes The
T13061 6456-6462 JJ denotes mutant
T13062 6463-6467 NN denotes mice
T13063 6468-6469 -LRB- denotes (
T13064 6469-6474 IN denotes above
T13065 6474-6475 -RRB- denotes )
T13066 6476-6483 VB denotes develop
T13067 6484-6495 JJ denotes progressive
T13068 6496-6500 NN denotes skin
T13069 6501-6513 NN denotes inflammation
T13070 6514-6522 VB denotes starting
T13071 6523-6525 IN denotes at
T13072 6526-6531 IN denotes about
T13073 6532-6536 CD denotes four
T13074 6537-6543 NN denotes weeks.
T13075 6544-6545 -LRB- denotes (
T13076 6545-6546 NN denotes C
T13077 6546-6547 -RRB- denotes )
T13078 6548-6562 JJ denotes Extramedullary
T13079 6563-6576 NN denotes hematopoiesis
T13080 6577-6583 VB denotes causes
T13081 6584-6590 JJ denotes marked
T13082 6591-6602 NN denotes enlargement
T13083 6603-6605 IN denotes of
T13084 6606-6609 DT denotes the
T13085 6610-6616 NN denotes spleen
T13086 6617-6619 IN denotes of
T13087 6620-6624 NN denotes cpdm
T13088 6625-6630 NN denotes mice.
T13089 6631-6632 -LRB- denotes (
T13090 6632-6633 NN denotes D
T13091 6633-6634 -RRB- denotes )
T13092 6635-6639 NN denotes BMDC
T13093 6640-6644 VB denotes were
T13094 6645-6654 VB denotes incubated
T13095 6655-6657 IN denotes in
T13096 6658-6661 DT denotes the
T13097 6662-6669 NN denotes absence
T13098 6670-6672 CC denotes or
T13099 6673-6681 NN denotes presence
T13100 6682-6684 IN denotes of
T13101 6685-6688 CD denotes 100
T13102 6689-6694 NN denotes ng/ml
T13103 6695-6698 NN denotes LPS
T13104 6699-6702 IN denotes for
T13105 6703-6704 CD denotes 4
T13106 6705-6710 NN denotes hours
T13107 6712-6715 DT denotes The
T13108 6716-6720 NN denotes mRNA
T13109 6721-6726 NN denotes level
T13110 6727-6729 IN denotes of
T13111 6730-6737 NN denotes Sharpin
T13112 6738-6741 VB denotes was
T13113 6742-6750 VB denotes measured
T13114 6751-6753 IN denotes by
T13115 6754-6761 NN denotes qRT-PCR
T13116 6762-6765 CC denotes and
T13117 6766-6775 VB denotes presented
T13118 6776-6784 JJ denotes relative
T13119 6785-6787 TO denotes to
T13120 6788-6791 DT denotes the
T13121 6792-6796 NN denotes mRNA
T13122 6797-6807 NN denotes expression
T13123 6808-6810 IN denotes in
T13124 6811-6825 JJ denotes non-stimulated
T13125 6826-6828 NNP denotes WT
T13126 6829-6833 NN denotes BMDC
T13127 6835-6839 NN denotes Bars
T13128 6840-6849 VB denotes represent
T13129 6850-6853 DT denotes the
T13130 6854-6858 JJ denotes mean
T13131 6859-6860 JJ denotes ±
T13132 6861-6865 NN denotes s.d.
T13133 6866-6868 IN denotes of
T13134 6869-6870 CD denotes 3
T13135 6871-6876 NN denotes mice.
T13136 6877-6878 SYM denotes *
T13137 6879-6880 NN denotes P
T13138 6880-6881 SYM denotes <
T13139 6881-6885 CD denotes 0.05
T13140 6885-6886 -COLON- denotes ;
T13141 6887-6889 SYM denotes **
T13142 6890-6891 NN denotes P
T13143 6891-6892 SYM denotes <
T13144 6892-6898 CD denotes 0.001.
T13145 6899-6900 -LRB- denotes (
T13146 6900-6901 NN denotes E
T13147 6901-6902 -RRB- denotes )
T13148 6903-6913 NN denotes Fibroblast
T13149 6914-6915 -LRB- denotes (
T13150 6915-6921 NN denotes NIH3T3
T13151 6921-6922 -RRB- denotes )
T13152 6923-6926 CC denotes and
T13153 6927-6938 NN denotes macrophages
T13154 6939-6940 -LRB- denotes (
T13155 6940-6948 NN denotes RAW264.7
T13156 6948-6949 -RRB- denotes )
T13157 6950-6955 NN denotes cells
T13158 6956-6960 VB denotes were
T13159 6961-6972 VB denotes transfected
T13160 6973-6977 IN denotes with
T13161 6978-6981 DT denotes the
T13162 6982-6992 NN denotes expression
T13163 6993-7000 NN denotes plasmid
T13164 7001-7014 NN denotes pFLAG-SHARPIN
T13165 7016-7021 IN denotes After
T13166 7022-7024 CD denotes 48
T13167 7025-7030 NN denotes hours
T13168 7030-7031 -COMMA- denotes ,
T13169 7032-7037 NN denotes cells
T13170 7038-7042 VB denotes were
T13171 7043-7048 VB denotes fixed
T13172 7049-7052 CC denotes and
T13173 7053-7059 VB denotes probed
T13174 7060-7064 IN denotes with
T13175 7065-7074 JJ denotes anti-FLAG
T13176 7075-7078 CC denotes and
T13177 7079-7094 JJ denotes FITC-conjugated
T13178 7095-7104 JJ denotes secondary
T13179 7105-7113 NN denotes antibody
T13180 7115-7121 NN denotes Nuclei
T13181 7122-7126 VB denotes were
T13182 7127-7134 VB denotes stained
T13183 7135-7139 IN denotes with
T13184 7140-7144 NNP denotes DAPI
T13185 7146-7148 IN denotes In
T13186 7149-7153 DT denotes both
T13187 7154-7165 VB denotes transfected
T13188 7166-7170 NN denotes cell
T13189 7171-7176 NN denotes lines
T13190 7176-7177 -COMMA- denotes ,
T13191 7178-7190 NN denotes FLAG-SHARPIN
T13192 7191-7194 VB denotes was
T13193 7195-7200 VB denotes found
T13194 7201-7203 TO denotes to
T13195 7204-7206 VB denotes be
T13196 7207-7226 JJ denotes cytoplasm-localized
T13537 8298-8304 NN denotes Effect
T13538 8305-8307 IN denotes of
T13539 8308-8315 NNP denotes Sharpin
T13540 8316-8324 NN denotes mutation
T13541 8325-8327 IN denotes on
T13542 8328-8330 NN denotes DC
T13543 8331-8345 NN denotes subpopulations
T13544 8346-8349 CC denotes and
T13545 8350-8360 NN denotes maturation
T13546 8362-8363 -LRB- denotes (
T13547 8363-8364 NN denotes A
T13548 8364-8365 -RRB- denotes )
T13549 8366-8373 NN denotes Spleens
T13550 8374-8378 IN denotes from
T13551 8379-8381 NN denotes WT
T13552 8382-8385 CC denotes and
T13553 8386-8390 NN denotes cpdm
T13554 8391-8395 NN denotes mice
T13555 8396-8400 VB denotes were
T13556 8401-8409 VB denotes isolated
T13557 8410-8413 CC denotes and
T13558 8414-8421 JJ denotes subject
T13559 8422-8424 TO denotes to
T13560 8425-8436 NN denotes collagenase
T13561 8437-8440 CC denotes and
T13562 8441-8446 NN denotes DNase
T13563 8447-8456 NN denotes digestion
T13564 8458-8461 DT denotes The
T13565 8462-8470 VB denotes obtained
T13566 8471-8478 JJ denotes splenic
T13567 8479-8490 NN denotes homogenates
T13568 8491-8495 VB denotes were
T13569 8496-8507 VB denotes centrifuged
T13570 8508-8512 IN denotes over
T13571 8513-8514 DT denotes a
T13572 8515-8522 NN denotes Percoll
T13573 8523-8531 NN denotes gradient
T13574 8532-8533 -LRB- denotes (
T13575 8533-8535 CD denotes 35
T13576 8535-8536 NN denotes %
T13577 8537-8540 CC denotes and
T13578 8541-8543 CD denotes 55
T13579 8543-8544 NN denotes %
T13580 8545-8552 NN denotes density
T13581 8552-8553 -RRB- denotes )
T13582 8554-8557 IN denotes for
T13583 8558-8560 CD denotes 15
T13584 8561-8568 NN denotes minutes
T13585 8570-8573 DT denotes The
T13586 8574-8579 NN denotes bands
T13587 8580-8582 IN denotes at
T13588 8583-8586 DT denotes the
T13589 8587-8589 CD denotes 35
T13590 8589-8590 NN denotes %
T13591 8590-8597 NN denotes -medium
T13592 8598-8601 CC denotes and
T13593 8602-8605 DT denotes the
T13594 8606-8611 CD denotes 35–55
T13595 8611-8612 NN denotes %
T13596 8613-8622 NN denotes interface
T13597 8623-8627 VB denotes were
T13598 8628-8634 VB denotes pooled
T13599 8634-8635 -COMMA- denotes ,
T13600 8636-8642 VB denotes washed
T13601 8643-8646 CC denotes and
T13602 8647-8654 VB denotes stained
T13603 8655-8659 IN denotes with
T13604 8660-8661 DT denotes a
T13605 8662-8673 NN denotes combination
T13606 8674-8676 IN denotes of
T13607 8677-8684 JJ denotes various
T13608 8685-8695 NN denotes antibodies
T13609 8696-8698 TO denotes to
T13610 8699-8704 VB denotes stain
T13611 8705-8714 JJ denotes different
T13612 8715-8717 NN denotes DC
T13613 8718-8725 NN denotes subsets
T13614 8725-8726 -COMMA- denotes ,
T13615 8727-8739 JJ denotes conventional
T13616 8740-8751 NN denotes CD11c+CD8α+
T13617 8751-8752 -COMMA- denotes ,
T13618 8753-8764 NN denotes CD11c+CD8α−
T13619 8765-8768 CC denotes and
T13620 8769-8781 JJ denotes plasmacytoid
T13621 8782-8784 NN denotes DC
T13622 8785-8786 -LRB- denotes (
T13623 8786-8799 JJ denotes CD11c−PDCA-1+
T13624 8799-8800 -RRB- denotes )
T13625 8800-8804 NN denotes .The
T13626 8805-8808 JJ denotes top
T13627 8809-8815 NN denotes panels
T13628 8816-8820 VB denotes were
T13629 8821-8826 VB denotes gated
T13630 8827-8829 IN denotes on
T13631 8830-8840 NN denotes FSChiSSClo
T13632 8841-8846 NN denotes cells
T13633 8847-8849 TO denotes to
T13634 8850-8854 VB denotes show
T13635 8855-8863 JJ denotes separate
T13636 8864-8875 NN denotes populations
T13637 8876-8878 IN denotes of
T13638 8879-8892 JJ denotes CD11c+PDCA-1−
T13639 8893-8896 CC denotes and
T13640 8897-8910 JJ denotes CD11c−PDCA-1+
T13641 8911-8916 NN denotes cells
T13642 8918-8925 RB denotes Further
T13643 8926-8932 VB denotes gating
T13644 8933-8935 IN denotes on
T13645 8936-8939 DT denotes the
T13646 8940-8953 JJ denotes CD11c+PDCA-1−
T13647 8954-8967 NN denotes subpopulation
T13648 8968-8972 VB denotes gave
T13649 8973-8976 DT denotes the
T13650 8977-8983 NN denotes bottom
T13651 8984-8989 NN denotes panel
T13652 8990-8994 WDT denotes that
T13653 8995-9001 VB denotes showed
T13654 9002-9005 CD denotes two
T13655 9006-9014 JJ denotes distinct
T13656 9015-9020 NN denotes pools
T13657 9021-9023 IN denotes of
T13658 9024-9035 JJ denotes CD11c+CD8α+
T13659 9036-9039 CC denotes and
T13660 9040-9051 NN denotes CD11c+CD8α−
T13661 9052-9057 NN denotes cells
T13662 9059-9070 NN denotes Percentages
T13663 9071-9075 VB denotes were
T13664 9076-9086 VB denotes calculated
T13665 9087-9092 VB denotes based
T13666 9093-9095 IN denotes on
T13667 9096-9099 DT denotes the
T13668 9100-9108 JJ denotes parental
T13669 9109-9119 NN denotes population
T13670 9120-9123 CC denotes and
T13671 9124-9128 VB denotes were
T13672 9129-9141 RB denotes additionally
T13673 9142-9147 VB denotes shown
T13674 9148-9150 IN denotes as
T13675 9151-9154 NN denotes bar
T13676 9155-9161 NN denotes graphs
T13677 9162-9163 -LRB- denotes (
T13678 9163-9168 NN denotes n = 2
T13679 9168-9169 -RRB- denotes )
T13680 9170-9171 -LRB- denotes (
T13681 9171-9172 NN denotes B
T13682 9172-9173 -RRB- denotes )
T13683 9173-9174 -LRB- denotes .
T13684 9175-9176 NN denotes (
T13685 9176-9177 -RRB- denotes C
T13686 9177-9179 NN denotes )
T13687 9179-9182 CC denotes WT
T13688 9182-9186 NN denotes and
T13689 9186-9190 NN denotes cpdm
T13690 9191-9192 -LRB- denotes B
T13691 9192-9197 CD denotes MDC (
T13692 9197-9198 -RRB- denotes 5
T13693 9198-9203 NN denotes ×105)
T13694 9204-9208 VB denotes cell
T13695 9208-9218 VB denotes s were sti
T13696 9218-9222 IN denotes mula
T13697 9222-9228 NN denotes ted wi
T13698 9228-9229 -COMMA- denotes t
T13699 9229-9232 CD denotes h m
T13700 9232-9237 NN denotes edium
T13701 9237-9240 NN denotes , 1
T13702 9240-9242 CC denotes 00
T13703 9243-9245 CD denotes ng
T13704 9245-9250 NN denotes /ml L
T13705 9250-9254 NN denotes PS o
T13706 9254-9255 NN denotes r
T13707 9256-9257 -COLON- denotes 2
T13708 9257-9258 NN denotes 5
T13709 9259-9262 IN denotes µg/
T13710 9262-9264 CD denotes ml
T13711 9265-9270 NN denotes poly
T13712 9288-9291 DT denotes The
T13713 9292-9297 NN denotes cells
T13714 9298-9302 VB denotes were
T13715 9303-9310 VB denotes labeled
T13716 9311-9315 IN denotes with
T13717 9316-9326 JJ denotes PE-labeled
T13718 9327-9336 NN denotes anti-CD40
T13719 9336-9337 -COMMA- denotes ,
T13720 9338-9347 NN denotes anti-CD80
T13721 9347-9348 -COMMA- denotes ,
T13722 9349-9352 CC denotes and
T13723 9353-9362 NN denotes anti-CD86
T13724 9362-9363 -COMMA- denotes ,
T13725 9364-9367 CC denotes and
T13726 9368-9377 VB denotes subjected
T13727 9378-9380 TO denotes to
T13728 9381-9385 VB denotes flow
T13729 9386-9395 NN denotes cytometry
T13730 9396-9404 NN denotes analysis
T13731 9406-9409 DT denotes The
T13732 9410-9421 NN denotes populations
T13733 9422-9427 VB denotes shown
T13734 9428-9430 IN denotes in
T13735 9431-9441 NN denotes histograms
T13736 9442-9446 VB denotes were
T13737 9447-9452 VB denotes gated
T13738 9453-9455 IN denotes on
T13739 9456-9462 JJ denotes CD11c+
T13740 9463-9468 NN denotes cells
T13741 9470-9479 JJ denotes Unstained
T13742 9480-9485 NN denotes cells
T13743 9486-9492 VB denotes served
T13744 9493-9495 IN denotes as
T13745 9496-9504 JJ denotes negative
T13746 9505-9513 NN denotes controls
T13747 9515-9522 NN denotes Results
T13748 9523-9526 VB denotes are
T13749 9527-9541 JJ denotes representative
T13750 9542-9544 IN denotes of
T13751 9545-9548 CD denotes two
T13752 9549-9560 JJ denotes independent
T13753 9561-9572 NN denotes experiments
T14018 11390-11399 JJ denotes Defective
T14019 11400-11410 NN denotes production
T14020 11411-11413 IN denotes of
T14021 11414-11430 JJ denotes pro-inflammatory
T14022 11431-11440 NN denotes mediators
T14023 11441-11445 IN denotes from
T14024 11446-11456 VB denotes stimulated
T14025 11457-11461 NN denotes cpdm
T14026 11462-11466 NN denotes BMDC
T14027 11468-11469 -LRB- denotes (
T14028 11469-11472 NN denotes A–D
T14029 11472-11473 -RRB- denotes )
T14030 11474-11482 JJ denotes Cultured
T14031 11483-11485 NN denotes WT
T14032 11486-11489 CC denotes and
T14033 11490-11494 NN denotes cpdm
T14034 11495-11499 NN denotes BMDC
T14035 11500-11501 -LRB- denotes (
T14036 11501-11506 CD denotes 1×105
T14037 11507-11512 NN denotes cells
T14038 11513-11515 IN denotes in
T14039 11516-11519 CD denotes 0.1
T14040 11520-11522 NN denotes ml
T14041 11523-11531 JJ denotes complete
T14042 11532-11538 NN denotes medium
T14043 11538-11539 -RRB- denotes )
T14044 11540-11544 VB denotes were
T14045 11545-11551 VB denotes washed
T14046 11552-11555 CC denotes and
T14047 11556-11566 VB denotes stimulated
T14048 11567-11571 IN denotes with
T14049 11572-11578 NN denotes medium
T14050 11578-11579 -COMMA- denotes ,
T14051 11580-11583 CD denotes 100
T14052 11584-11589 NN denotes ng/ml
T14053 11590-11593 NN denotes LPS
T14054 11594-11596 CC denotes or
T14055 11597-11599 CD denotes 25
T14056 11600-11605 NN denotes µg/ml
T14057 11606-11610 NN denotes poly
T14058 11611-11612 NN denotes I
T14059 11612-11613 -COLON- denotes :
T14060 11613-11614 NN denotes C
T14061 11615-11618 IN denotes for
T14062 11619-11621 CD denotes 24
T14063 11622-11627 NN denotes hours
T14064 11629-11641 NN denotes Supernatants
T14065 11642-11646 VB denotes were
T14066 11647-11656 VB denotes collected
T14067 11657-11660 IN denotes for
T14068 11661-11666 NN denotes ELISA
T14069 11667-11669 IN denotes of
T14070 11670-11677 NN denotes IL12P70
T14071 11677-11678 -COMMA- denotes ,
T14072 11679-11682 NN denotes IL6
T14073 11682-11683 -COMMA- denotes ,
T14074 11684-11687 CC denotes and
T14075 11688-11693 NN denotes GMCSF
T14076 11693-11694 -COMMA- denotes ,
T14077 11695-11698 CC denotes and
T14078 11699-11702 IN denotes for
T14079 11703-11717 NN denotes quantification
T14080 11718-11720 IN denotes of
T14081 11721-11727 JJ denotes nitric
T14082 11728-11733 NN denotes oxide
T14083 11734-11735 -LRB- denotes (
T14084 11735-11737 NN denotes NO
T14085 11737-11738 -RRB- denotes )
T14086 11738-11739 -LRB- denotes .
T14087 11740-11743 NN denotes (E–
T14088 11743-11744 -RRB- denotes F
T14089 11744-11752 NN denotes ) Gradie
T14090 11752-11759 NN denotes nt numb
T14091 11759-11760 -LRB- denotes e
T14092 11760-11765 CD denotes rs (1
T14093 11765-11766 -COMMA- denotes ×
T14094 11766-11771 CD denotes 104,
T14095 11771-11772 -COMMA- denotes 2
T14096 11772-11775 CC denotes ×10
T14097 11775-11780 CD denotes 4, an
T14098 11780-11785 NN denotes d 4×1
T14099 11785-11787 IN denotes 04
T14100 11788-11791 CD denotes cel
T14101 11791-11793 NN denotes ls
T14102 11794-11802 JJ denotes in 0.1 m
T14103 11802-11808 NN denotes L comp
T14104 11808-11809 -RRB- denotes l
T14105 11809-11811 IN denotes et
T14106 11811-11815 NN denotes e me
T14107 11815-11819 VB denotes dium
T14108 11819-11823 VB denotes ) of
T14109 11824-11830 NN denotes BMDC w
T14110 11830-11835 NN denotes ere u
T14111 11835-11838 NN denotes sed
T14112 11839-11850 NN denotes for100 ng/m
T14113 11869-11874 IN denotes After
T14114 11875-11877 CD denotes 24
T14115 11878-11883 NN denotes hours
T14116 11883-11884 -COMMA- denotes ,
T14117 11885-11888 DT denotes the
T14118 11889-11896 NN denotes amounts
T14119 11897-11899 IN denotes of
T14120 11900-11907 NN denotes IL12P70
T14121 11908-11911 CC denotes and
T14122 11912-11915 NN denotes IL6
T14123 11916-11920 IN denotes from
T14124 11921-11924 DT denotes the
T14125 11925-11936 NN denotes supernatant
T14126 11937-11941 VB denotes were
T14127 11942-11951 VB denotes measured.
T14128 11952-11953 -LRB- denotes (
T14129 11953-11954 NN denotes G
T14130 11954-11955 -RRB- denotes )
T14131 11956-11959 DT denotes The
T14132 11960-11964 NN denotes cpdm
T14133 11965-11969 NN denotes mice
T14134 11970-11977 VB denotes rescued
T14135 11978-11980 IN denotes by
T14136 11981-11999 JJ denotes Sharpin-containing
T14137 12000-12003 NN denotes BAC
T14138 12004-12007 VB denotes had
T14139 12008-12016 JJ denotes complete
T14140 12017-12026 NN denotes remission
T14141 12027-12029 IN denotes of
T14142 12030-12033 DT denotes the
T14143 12034-12046 JJ denotes inflammatory
T14144 12047-12056 NN denotes phenotype
T14145 12057-12058 -LRB- denotes [
T14146 12058-12059 CD denotes 2
T14147 12059-12060 -RRB- denotes ]
T14148 12062-12066 NN denotes BMDC
T14149 12067-12076 VB denotes developed
T14150 12077-12081 IN denotes from
T14151 12082-12086 NN denotes cpdm
T14152 12087-12090 CC denotes and
T14153 12091-12098 VB denotes rescued
T14154 12099-12103 NN denotes cpdm
T14155 12104-12108 NN denotes mice
T14156 12109-12113 VB denotes were
T14157 12114-12120 VB denotes plated
T14158 12121-12122 -LRB- denotes (
T14159 12122-12127 CD denotes 1×106
T14160 12128-12133 NN denotes cells
T14161 12134-12136 IN denotes in
T14162 12137-12140 CD denotes 0.3
T14163 12141-12143 NN denotes mL
T14164 12143-12144 -RRB- denotes )
T14165 12145-12148 CC denotes and
T14166 12149-12159 VB denotes stimulated
T14167 12160-12164 IN denotes with
T14168 12165-12168 CD denotes 100
T14169 12169-12174 NN denotes ng/mL
T14170 12175-12178 NN denotes LPS
T14171 12180-12185 IN denotes After
T14172 12186-12188 CD denotes 24
T14173 12189-12194 NN denotes hours
T14174 12194-12195 -COMMA- denotes ,
T14175 12196-12208 NN denotes supernatants
T14176 12209-12213 VB denotes were
T14177 12214-12223 VB denotes collected
T14178 12224-12227 IN denotes for
T14179 12228-12236 NN denotes analysis
T14180 12237-12239 IN denotes of
T14181 12240-12247 NN denotes IL12P70
T14182 12248-12258 NN denotes production
T14183 12260-12264 NN denotes Data
T14184 12265-12268 VB denotes are
T14185 12269-12283 JJ denotes representative
T14186 12284-12286 IN denotes of
T14187 12287-12292 CD denotes three
T14188 12293-12304 JJ denotes independent
T14189 12305-12317 NN denotes experiments.
T14190 12318-12319 SYM denotes *
T14191 12320-12321 NN denotes P
T14192 12321-12322 SYM denotes <
T14193 12322-12326 CD denotes 0.01
T14194 12326-12327 -COLON- denotes ;
T14195 12328-12330 SYM denotes **
T14196 12331-12332 NN denotes P
T14197 12332-12333 SYM denotes <
T14198 12333-12338 CD denotes 0.005
T14388 12384-12393 VB denotes Decreased
T14389 12394-12398 NN denotes mRNA
T14390 12399-12405 NN denotes levels
T14391 12406-12408 IN denotes of
T14392 12409-12421 JJ denotes inflammatory
T14393 12422-12431 NN denotes cytokines
T14394 12432-12436 IN denotes from
T14395 12437-12441 NN denotes cpdm
T14396 12442-12446 NN denotes BMDC
T14397 12448-12456 VB denotes Cultured
T14398 12457-12459 NN denotes WT
T14399 12460-12463 CC denotes and
T14400 12464-12468 NN denotes cpdm
T14401 12469-12473 NN denotes BMDC
T14402 12474-12475 -LRB- denotes (
T14403 12475-12480 CD denotes 5×105
T14404 12481-12486 NN denotes cells
T14405 12487-12489 IN denotes in
T14406 12490-12493 CD denotes 0.2
T14407 12494-12496 NN denotes ml
T14408 12497-12505 JJ denotes complete
T14409 12506-12512 NN denotes medium
T14410 12512-12513 -RRB- denotes )
T14411 12514-12518 VB denotes were
T14412 12519-12525 VB denotes washed
T14413 12526-12529 CC denotes and
T14414 12530-12540 VB denotes stimulated
T14415 12541-12545 IN denotes with
T14416 12546-12549 CD denotes 100
T14417 12550-12555 NN denotes ng/ml
T14418 12556-12559 NN denotes LPS
T14419 12560-12561 -LRB- denotes (
T14420 12561-12562 NN denotes A
T14421 12562-12563 -COMMA- denotes ,
T14422 12563-12564 NN denotes C
T14423 12564-12565 -COMMA- denotes ,
T14424 12565-12566 NN denotes E
T14425 12566-12567 -COMMA- denotes ,
T14426 12567-12568 NN denotes G
T14427 12568-12569 -RRB- denotes )
T14428 12570-12572 CC denotes or
T14429 12573-12575 CD denotes 25
T14430 12576-12581 NN denotes µg/ml
T14431 12582-12586 NN denotes poly
T14432 12587-12588 NN denotes I
T14433 12588-12589 SYM denotes :
T14434 12589-12590 NN denotes C
T14435 12591-12592 -LRB- denotes (
T14436 12592-12593 NN denotes B
T14437 12593-12594 -COMMA- denotes ,
T14438 12594-12595 NNP denotes D
T14439 12595-12596 -COMMA- denotes ,
T14440 12596-12597 NNP denotes F
T14441 12597-12598 -COMMA- denotes ,
T14442 12598-12599 NN denotes H
T14443 12599-12600 -RRB- denotes )
T14444 12602-12604 IN denotes At
T14445 12605-12606 CD denotes 0
T14446 12606-12607 -COMMA- denotes ,
T14447 12608-12609 CD denotes 1
T14448 12610-12613 CC denotes and
T14449 12614-12615 CD denotes 2
T14450 12616-12621 NN denotes hours
T14451 12621-12622 -COMMA- denotes ,
T14452 12623-12628 JJ denotes total
T14453 12629-12632 NN denotes RNA
T14454 12633-12636 VB denotes was
T14455 12637-12646 VB denotes extracted
T14456 12647-12650 CC denotes and
T14457 12651-12658 JJ denotes subject
T14458 12659-12661 TO denotes to
T14459 12662-12669 NN denotes qRT-PCR
T14460 12670-12672 TO denotes to
T14461 12673-12680 VB denotes measure
T14462 12681-12684 DT denotes the
T14463 12685-12695 NN denotes expression
T14464 12696-12698 IN denotes of
T14465 12699-12706 NN denotes Il12p40
T14466 12707-12708 -LRB- denotes (
T14467 12708-12709 NN denotes A
T14468 12709-12710 -COMMA- denotes ,
T14469 12710-12711 NN denotes B
T14470 12711-12712 -RRB- denotes )
T14471 12712-12713 -COMMA- denotes ,
T14472 12714-12717 NN denotes Il6
T14473 12718-12719 -LRB- denotes (
T14474 12719-12720 NN denotes C
T14475 12720-12721 -COMMA- denotes ,
T14476 12721-12722 NN denotes D
T14477 12722-12723 -RRB- denotes )
T14478 12723-12724 -COMMA- denotes ,
T14479 12725-12730 NN denotes Gmcsf
T14480 12731-12732 -LRB- denotes (
T14481 12732-12733 NN denotes E
T14482 12733-12734 -COMMA- denotes ,
T14483 12734-12735 NN denotes F
T14484 12735-12736 -RRB- denotes )
T14485 12736-12737 -COMMA- denotes ,
T14486 12738-12741 CC denotes and
T14487 12742-12746 NN denotes Ifnb
T14488 12747-12748 -LRB- denotes (
T14489 12748-12749 NN denotes G
T14490 12749-12750 -COMMA- denotes ,
T14491 12750-12751 NN denotes H
T14492 12751-12752 -RRB- denotes )
T14493 12753-12757 NN denotes mRNA
T14494 12759-12763 NN denotes Bars
T14495 12764-12773 VB denotes represent
T14496 12774-12778 JJ denotes mean
T14497 12779-12780 JJ denotes ±
T14498 12781-12783 NN denotes SD
T14499 12785-12789 NN denotes Data
T14500 12790-12793 VB denotes are
T14501 12794-12808 JJ denotes representative
T14502 12809-12811 IN denotes of
T14503 12812-12815 CD denotes two
T14504 12816-12827 JJ denotes independent
T14505 12828-12839 NN denotes experiments
T14698 14284-14290 JJ denotes Normal
T14699 14291-14295 NN denotes TLR4
T14700 14296-14299 CC denotes and
T14701 14300-14303 NN denotes MD2
T14702 14304-14314 NN denotes expression
T14703 14315-14318 CC denotes and
T14704 14319-14328 VB denotes decreased
T14705 14329-14333 NN denotes IL10
T14706 14334-14343 NN denotes secretion
T14707 14344-14347 CC denotes and
T14708 14348-14351 NN denotes A20
T14709 14352-14362 NN denotes expression
T14710 14363-14365 IN denotes by
T14711 14366-14370 NN denotes cpdm
T14712 14371-14375 NN denotes BMDC
T14713 14377-14378 -LRB- denotes (
T14714 14378-14379 NN denotes A
T14715 14379-14380 -RRB- denotes )
T14716 14381-14393 JJ denotes Unstimulated
T14717 14394-14396 NN denotes WT
T14718 14397-14400 CC denotes and
T14719 14401-14405 NN denotes cpdm
T14720 14406-14410 NN denotes BMDC
T14721 14411-14412 -LRB- denotes (
T14722 14412-14417 CD denotes 5×105
T14723 14417-14418 -RRB- denotes )
T14724 14419-14423 VB denotes were
T14725 14424-14431 VB denotes labeled
T14726 14432-14436 IN denotes with
T14727 14437-14447 JJ denotes PE-labeled
T14728 14448-14461 NN denotes anti-TLR4/MD2
T14729 14462-14464 CC denotes or
T14730 14465-14474 NN denotes anti-CD14
T14731 14475-14478 CC denotes and
T14732 14479-14483 RB denotes then
T14733 14484-14491 JJ denotes subject
T14734 14492-14494 TO denotes to
T14735 14495-14499 VB denotes flow
T14736 14500-14509 NN denotes cytometry
T14737 14510-14518 NN denotes analysis
T14738 14520-14529 JJ denotes Unstained
T14739 14530-14535 NN denotes cells
T14740 14536-14541 VB denotes serve
T14741 14542-14544 IN denotes as
T14742 14545-14553 JJ denotes negative
T14743 14554-14563 NN denotes controls.
T14744 14564-14565 -LRB- denotes (
T14745 14565-14566 NN denotes B
T14746 14566-14567 -RRB- denotes )
T14747 14568-14576 JJ denotes Cultured
T14748 14577-14579 NN denotes WT
T14749 14580-14583 CC denotes and
T14750 14584-14588 NN denotes cpdm
T14751 14589-14593 NN denotes BMDC
T14752 14594-14595 -LRB- denotes (
T14753 14595-14600 CD denotes 1×105
T14754 14601-14606 NN denotes cells
T14755 14607-14609 IN denotes in
T14756 14610-14613 CD denotes 0.1
T14757 14614-14616 NN denotes ml
T14758 14617-14625 JJ denotes complete
T14759 14626-14632 NN denotes medium
T14760 14632-14633 -RRB- denotes )
T14761 14634-14638 VB denotes were
T14762 14639-14645 VB denotes washed
T14763 14646-14649 CC denotes and
T14764 14650-14660 VB denotes stimulated
T14765 14661-14665 IN denotes with
T14766 14666-14672 NN denotes medium
T14767 14672-14673 -COMMA- denotes ,
T14768 14674-14677 CD denotes 100
T14769 14678-14683 NN denotes ng/ml
T14770 14684-14687 NN denotes LPS
T14771 14688-14690 CC denotes or
T14772 14691-14693 CD denotes 25
T14773 14694-14699 NN denotes µg/ml
T14774 14700-14704 NN denotes poly
T14775 14705-14706 NN denotes I
T14776 14706-14707 -COLON- denotes :
T14777 14707-14708 NN denotes C
T14778 14709-14712 IN denotes for
T14779 14713-14715 CD denotes 24
T14780 14716-14721 NN denotes hours
T14781 14723-14735 NN denotes Supernatants
T14782 14736-14740 VB denotes were
T14783 14741-14750 VB denotes collected
T14784 14751-14754 IN denotes for
T14785 14755-14760 NN denotes ELISA
T14786 14761-14763 IN denotes of
T14787 14764-14769 NN denotes IL10.
T14788 14770-14771 -LRB- denotes (
T14789 14771-14772 NN denotes C
T14790 14772-14773 -RRB- denotes )
T14791 14774-14782 JJ denotes Cultured
T14792 14783-14785 NN denotes WT
T14793 14786-14789 CC denotes and
T14794 14790-14794 NN denotes cpdm
T14795 14795-14799 NN denotes BMDC
T14796 14800-14801 -LRB- denotes (
T14797 14801-14806 CD denotes 5×105
T14798 14807-14812 NN denotes cells
T14799 14813-14815 IN denotes in
T14800 14816-14819 CD denotes 0.2
T14801 14820-14822 NN denotes ml
T14802 14823-14831 JJ denotes complete
T14803 14832-14838 NN denotes medium
T14804 14838-14839 -RRB- denotes )
T14805 14840-14844 VB denotes were
T14806 14845-14851 VB denotes washed
T14807 14852-14855 CC denotes and
T14808 14856-14866 VB denotes stimulated
T14809 14867-14871 IN denotes with
T14810 14872-14875 CD denotes 100
T14811 14876-14881 NN denotes ng/ml
T14812 14882-14885 NN denotes LPS
T14813 14886-14888 CC denotes or
T14814 14889-14891 CD denotes 25
T14815 14892-14897 NN denotes µg/ml
T14816 14898-14902 NN denotes poly
T14817 14903-14904 CD denotes I
T14818 14904-14905 -COLON- denotes :
T14819 14905-14907 NNP denotes C.
T14820 14908-14910 IN denotes At
T14821 14911-14912 CD denotes 0
T14822 14912-14913 -COMMA- denotes ,
T14823 14914-14915 CD denotes 1
T14824 14915-14916 -COMMA- denotes ,
T14825 14917-14920 CC denotes and
T14826 14921-14922 CD denotes 2
T14827 14923-14928 NN denotes hours
T14828 14928-14929 -COMMA- denotes ,
T14829 14930-14935 JJ denotes total
T14830 14936-14939 NN denotes RNA
T14831 14940-14943 VB denotes was
T14832 14944-14953 VB denotes extracted
T14833 14954-14957 CC denotes and
T14834 14958-14965 JJ denotes subject
T14835 14966-14968 TO denotes to
T14836 14969-14976 NN denotes qRT-PCR
T14837 14977-14979 TO denotes to
T14838 14980-14987 VB denotes measure
T14839 14988-14991 DT denotes the
T14840 14992-15002 NN denotes production
T14841 15003-15005 IN denotes of
T14842 15006-15009 NN denotes A20
T14998 16085-16095 NN denotes Inhibition
T14999 16096-16098 IN denotes of
T15000 16099-16104 NN denotes NF-κB
T15001 16105-16114 NN denotes signaling
T15002 16115-16117 IN denotes in
T15003 16118-16122 NN denotes cpdm
T15004 16123-16127 NN denotes BMDC
T15005 16129-16131 NN denotes WT
T15006 16132-16135 CC denotes and
T15007 16136-16140 NN denotes cpdm
T15008 16141-16145 NN denotes BMDC
T15009 16146-16147 -LRB- denotes (
T15010 16147-16152 CD denotes 2×106
T15011 16153-16158 NN denotes cells
T15012 16159-16161 IN denotes in
T15013 16162-16165 CD denotes 0.5
T15014 16166-16168 NN denotes mL
T15015 16169-16177 JJ denotes complete
T15016 16178-16184 NN denotes medium
T15017 16184-16185 -RRB- denotes )
T15018 16186-16190 VB denotes were
T15019 16191-16201 VB denotes stimulated
T15020 16202-16206 IN denotes with
T15021 16207-16210 CD denotes 100
T15022 16211-16216 NN denotes ng/mL
T15023 16217-16220 NN denotes LPS
T15024 16221-16222 -LRB- denotes (
T15025 16222-16223 NN denotes A
T15026 16223-16224 -RRB- denotes )
T15027 16225-16227 CC denotes or
T15028 16228-16230 CD denotes 25
T15029 16231-16236 NN denotes µg/mL
T15030 16237-16241 NN denotes poly
T15031 16242-16243 NN denotes I
T15032 16243-16244 SYM denotes :
T15033 16244-16245 NN denotes C
T15034 16246-16247 -LRB- denotes (
T15035 16247-16248 NN denotes B
T15036 16248-16249 -RRB- denotes )
T15037 16251-16253 IN denotes At
T15038 16254-16255 CD denotes 0
T15039 16255-16256 -COMMA- denotes ,
T15040 16257-16259 CD denotes 15
T15041 16259-16260 -COMMA- denotes ,
T15042 16261-16263 CD denotes 30
T15043 16263-16264 -COMMA- denotes ,
T15044 16265-16268 CC denotes and
T15045 16269-16271 CD denotes 60
T15046 16272-16279 NN denotes minutes
T15047 16279-16280 -COMMA- denotes ,
T15048 16281-16291 JJ denotes whole-cell
T15049 16292-16299 NN denotes lysates
T15050 16300-16304 VB denotes were
T15051 16305-16313 VB denotes obtained
T15052 16314-16317 CC denotes and
T15053 16318-16325 JJ denotes subject
T15054 16326-16328 TO denotes to
T15055 16329-16340 NN denotes immunoblots
T15056 16341-16345 IN denotes with
T15057 16346-16356 NN denotes antibodies
T15058 16357-16364 IN denotes against
T15059 16365-16373 NN denotes proteins
T15060 16374-16382 VB denotes involved
T15061 16383-16385 IN denotes in
T15062 16386-16391 NN denotes NF-κB
T15063 16391-16392 -COMMA- denotes ,
T15064 16393-16402 NN denotes TBK1/IRF3
T15065 16402-16403 -COMMA- denotes ,
T15066 16404-16410 NN denotes ERK1/2
T15067 16410-16411 -COMMA- denotes ,
T15068 16412-16415 CC denotes and
T15069 16416-16419 NN denotes p38
T15070 16420-16429 NN denotes signaling
T15071 16430-16438 NN denotes pathways
T15072 16440-16450 NN denotes Beta-actin
T15073 16451-16454 VB denotes was
T15074 16455-16459 VB denotes used
T15075 16460-16462 IN denotes as
T15076 16463-16470 VB denotes loading
T15077 16471-16479 NN denotes control.
T15078 16480-16481 -LRB- denotes (
T15079 16481-16482 NN denotes C
T15080 16482-16483 -RRB- denotes )
T15081 16484-16492 JJ denotes Cellular
T15082 16493-16499 NN denotes levels
T15083 16500-16502 IN denotes of
T15084 16503-16511 NN denotes p-IKK1/2
T15085 16512-16515 CC denotes and
T15086 16516-16522 NN denotes p-IκBα
T15087 16523-16525 IN denotes in
T15088 16526-16530 NN denotes LPS-
T15089 16531-16533 CC denotes or
T15090 16534-16538 NN denotes poly
T15091 16539-16540 CD denotes I
T15092 16540-16541 -COLON- denotes :
T15093 16541-16553 JJ denotes C-stimulated
T15094 16554-16558 NN denotes BMDC
T15095 16559-16563 VB denotes were
T15096 16564-16575 VB denotes quantitated
T15097 16576-16580 IN denotes with
T15098 16581-16587 NN denotes ImageJ
T15099 16588-16589 -LRB- denotes (
T15100 16589-16592 NN denotes NIH
T15101 16592-16593 -RRB- denotes )
T15102 16594-16597 CC denotes and
T15103 16598-16607 VB denotes presented
T15104 16608-16610 IN denotes as
T15105 16611-16616 NN denotes trend
T15106 16617-16622 NN denotes lines
T15107 16624-16631 NN denotes Results
T15108 16632-16635 VB denotes are
T15109 16636-16650 JJ denotes representative
T15110 16651-16653 IN denotes of
T15111 16654-16656 IN denotes at
T15112 16657-16662 JJ denotes least
T15113 16663-16666 CD denotes two
T15114 16667-16678 JJ denotes independent
T15115 16679-16690 NN denotes experiments
T15341 18492-18502 VB denotes Stimulated
T15342 18503-18507 NN denotes cpdm
T15343 18508-18512 NN denotes BMDC
T15344 18513-18520 VB denotes induced
T15345 18521-18531 JJ denotes Th2-biased
T15346 18532-18540 NN denotes cytokine
T15347 18541-18551 NN denotes production
T15348 18552-18556 IN denotes from
T15349 18557-18562 NN denotes naïve
T15350 18563-18567 JJ denotes CD4+
T15351 18568-18569 NN denotes T
T15352 18570-18575 NN denotes cells
T15353 18577-18579 NN denotes WT
T15354 18580-18583 CC denotes and
T15355 18584-18588 NN denotes cpdm
T15356 18589-18593 NN denotes BMDC
T15357 18594-18595 -LRB- denotes (
T15358 18595-18600 CD denotes 5×104
T15359 18601-18606 NN denotes cells
T15360 18607-18609 IN denotes in
T15361 18610-18613 CD denotes 0.1
T15362 18614-18616 NN denotes mL
T15363 18617-18625 JJ denotes complete
T15364 18626-18632 NN denotes medium
T15365 18632-18633 -COLON- denotes ;
T15366 18634-18637 NN denotes MHC
T15367 18638-18647 NN denotes haplotype
T15368 18647-18648 -COLON- denotes :
T15369 18649-18653 NN denotes H-2b
T15370 18653-18654 -RRB- denotes )
T15371 18655-18659 VB denotes were
T15372 18660-18669 VB denotes incubated
T15373 18670-18674 IN denotes with
T15374 18675-18681 NN denotes medium
T15375 18681-18682 -COMMA- denotes ,
T15376 18683-18686 CD denotes 100
T15377 18687-18692 NN denotes ng/mL
T15378 18693-18696 NN denotes LPS
T15379 18696-18697 -COMMA- denotes ,
T15380 18698-18700 CD denotes 25
T15381 18701-18706 NN denotes µg/mL
T15382 18707-18711 NN denotes poly
T15383 18712-18713 NN denotes I
T15384 18713-18714 -COLON- denotes :
T15385 18714-18715 NN denotes C
T15386 18716-18718 CC denotes or
T15387 18719-18720 CD denotes 5
T15388 18721-18726 NN denotes µg/mL
T15389 18727-18734 NN denotes Pam3CYS
T15390 18736-18741 IN denotes After
T15391 18742-18744 CD denotes 24
T15392 18745-18750 NN denotes hours
T15393 18750-18751 -COMMA- denotes ,
T15394 18752-18757 NN denotes cells
T15395 18758-18762 VB denotes were
T15396 18763-18769 VB denotes washed
T15397 18770-18774 IN denotes with
T15398 18775-18778 NNP denotes PBS
T15399 18779-18782 CC denotes and
T15400 18783-18792 VB denotes incubated
T15401 18793-18797 IN denotes with
T15402 18798-18805 RB denotes freshly
T15403 18806-18814 VB denotes isolated
T15404 18815-18825 JJ denotes allogeneic
T15405 18826-18831 NN denotes naïve
T15406 18832-18836 JJ denotes CD4+
T15407 18837-18838 NN denotes T
T15408 18839-18844 NN denotes cells
T15409 18845-18846 -LRB- denotes (
T15410 18846-18853 CD denotes 2.5×105
T15411 18854-18859 NN denotes cells
T15412 18860-18862 IN denotes in
T15413 18863-18866 CD denotes 0.1
T15414 18867-18869 NN denotes mL
T15415 18870-18878 JJ denotes complete
T15416 18879-18885 NN denotes medium
T15417 18885-18886 -COLON- denotes ;
T15418 18887-18890 NN denotes MHC
T15419 18891-18900 NN denotes haplotype
T15420 18900-18901 -COLON- denotes :
T15421 18902-18906 NN denotes H-2d
T15422 18906-18907 -RRB- denotes )
T15423 18909-18914 IN denotes After
T15424 18915-18916 CD denotes 5
T15425 18917-18921 NN denotes days
T15426 18921-18922 -COMMA- denotes ,
T15427 18923-18935 NN denotes supernatants
T15428 18936-18940 VB denotes were
T15429 18941-18950 VB denotes collected
T15430 18951-18954 CC denotes and
T15431 18955-18958 DT denotes the
T15432 18959-18968 NN denotes secretion
T15433 18969-18971 IN denotes of
T15434 18972-18976 NN denotes IFNγ
T15435 18976-18977 -COMMA- denotes ,
T15436 18978-18981 NN denotes IL4
T15437 18981-18982 -COMMA- denotes ,
T15438 18983-18986 CC denotes and
T15439 18987-18990 NN denotes IL2
T15440 18991-18995 VB denotes were
T15441 18996-19004 VB denotes measured
T15442 19005-19007 IN denotes by
T15443 19008-19013 NN denotes ELISA
T15444 19015-19023 JJ denotes Negative
T15445 19024-19032 NN denotes controls
T15446 19033-19036 VB denotes are
T15447 19037-19038 CD denotes 1
T15448 19038-19039 -RRB- denotes )
T15449 19040-19050 VB denotes stimulated
T15450 19051-19055 NN denotes BMDC
T15451 19056-19063 IN denotes without
T15452 19064-19074 NN denotes co-culture
T15453 19075-19079 IN denotes with
T15454 19080-19090 JJ denotes allogeneic
T15455 19091-19095 JJ denotes CD4+
T15456 19096-19097 NN denotes T
T15457 19098-19103 NN denotes cells
T15458 19103-19104 -COLON- denotes ;
T15459 19105-19106 LS denotes 2
T15460 19106-19107 -RRB- denotes )
T15461 19108-19118 JJ denotes allogeneic
T15462 19119-19123 JJ denotes CD4+
T15463 19124-19125 NN denotes T
T15464 19126-19131 NN denotes cells
T15465 19132-19139 IN denotes without
T15466 19140-19150 NN denotes co-culture
T15467 19151-19155 IN denotes with
T15468 19156-19166 VB denotes stimulated
T15469 19167-19171 NN denotes BMDC
T15470 19173-19180 NN denotes Samples
T15471 19181-19185 IN denotes from
T15472 19186-19190 CC denotes both
T15473 19191-19199 JJ denotes negative
T15474 19200-19208 NN denotes controls
T15475 19209-19212 VB denotes had
T15476 19213-19215 DT denotes no
T15477 19216-19226 JJ denotes detectable
T15478 19227-19237 NN denotes production
T15479 19238-19240 IN denotes of
T15480 19241-19244 DT denotes the
T15481 19245-19259 JJ denotes aforementioned
T15482 19260-19269 NN denotes cytokines
T15483 19270-19271 -LRB- denotes (
T15484 19271-19274 RB denotes not
T15485 19275-19280 VB denotes shown
T15486 19280-19281 -RRB- denotes )
T15487 19283-19290 NN denotes Results
T15488 19291-19294 VB denotes are
T15489 19295-19303 VB denotes analyzed
T15490 19304-19309 VB denotes based
T15491 19310-19312 IN denotes on
T15492 19313-19316 CD denotes 2–4
T15493 19317-19321 NN denotes mice
T15494 19322-19325 IN denotes per
T15495 19326-19332 NN denotes group.
T15496 19333-19334 SYM denotes *
T15497 19335-19336 NN denotes P
T15498 19336-19337 SYM denotes <
T15499 19337-19341 CD denotes 0.05
T12218 31854-31872 NN denotes Immunofluorescence
T12219 31873-31883 NN denotes Fibroblast
T12220 31884-31887 CC denotes and
T12221 31888-31898 NN denotes macrophage
T12222 31899-31904 NN denotes cells
T12223 31905-31909 VB denotes were
T12224 31910-31921 VB denotes transfected
T12225 31922-31926 IN denotes with
T12226 31927-31940 NN denotes pFLAG-SHARPIN
T12227 31942-31944 CD denotes 48
T12228 31945-31950 NN denotes hours
T12229 31951-31956 RB denotes later
T12230 31956-31957 -COMMA- denotes ,
T12231 31958-31963 NN denotes cells
T12232 31964-31968 VB denotes were
T12233 31969-31975 VB denotes washed
T12234 31976-31983 RB denotes briefly
T12235 31984-31988 IN denotes with
T12236 31989-31992 NNP denotes PBS
T12237 31992-31993 -COMMA- denotes ,
T12238 31994-31998 RB denotes then
T12239 31999-32004 VB denotes fixed
T12240 32005-32007 IN denotes in
T12241 32008-32012 JJ denotes cold
T9602 26152-26158 NNP denotes Ethics
T9603 26159-26168 NNP denotes Statement
T9604 26169-26172 DT denotes All
T9605 26173-26178 NN denotes mouse
T9606 26179-26183 NN denotes work
T9607 26184-26187 VB denotes was
T9608 26188-26195 VB denotes carried
T9609 26196-26199 RP denotes out
T9610 26200-26202 IN denotes in
T9611 26203-26209 JJ denotes strict
T9612 26210-26220 NN denotes accordance
T9613 26221-26225 IN denotes with
T9614 26226-26235 NN denotes protocols
T9615 26236-26244 VB denotes approved
T9616 26245-26247 IN denotes by
T9617 26248-26251 DT denotes the
T9618 26252-26265 NNP denotes Institutional
T9619 26266-26272 NNP denotes Animal
T9620 26273-26277 NNP denotes Care
T9621 26278-26281 CC denotes and
T9622 26282-26285 NNP denotes Use
T9623 26286-26296 NNP denotes Committees
T12019 31558-31560 IN denotes at
T12020 31561-31564 CD denotes 106
T12021 31565-31567 CC denotes or
T12022 31568-31571 CD denotes 105
R315 T399 T400 arg1Of SHARPIN,Is
R316 T399 T401 arg1Of SHARPIN,Essential
R317 T401 T400 arg2Of Essential,Is
R318 T401 T402 arg1Of Essential,for
R319 T404 T403 arg1Of Production,Cytokine
R320 T404 T405 arg1Of Production,","
R321 T405 T409 arg1Of ",",and
R322 T407 T405 arg2Of Signaling,","
R323 T407 T406 arg1Of Signaling,NF-κB
R324 T409 T402 arg2Of and,for
R325 T409 T408 arg1Of and,","
R326 T410 T409 arg2Of Induction,and
R327 T410 T411 arg1Of Induction,of
R328 T410 T414 arg1Of Induction,by
R329 T413 T411 arg2Of Differentiation,of
R330 T413 T412 arg1Of Differentiation,Th1
R331 T416 T414 arg2Of Cells,by
R332 T416 T415 arg1Of Cells,Dendritic
R333 T418 T417 arg1Of mutations,Spontaneous
R334 T418 T419 arg1Of mutations,of
R335 T426 T419 arg2Of protein,of
R336 T426 T420 arg1Of protein,the
R337 T426 T421 arg1Of protein,Sharpin
R338 T426 T422 arg1Of protein,(
R339 T426 T423 arg1Of protein,SHANK-associated
R340 T426 T424 arg1Of protein,RH
R341 T426 T425 arg1Of protein,domain-interacting
R342 T426 T427 arg1Of protein,","
R343 T429 T427 arg2Of aliases,","
R344 T429 T428 arg1Of aliases,other
R345 T429 T430 arg1Of aliases,:
R346 T431 T432 arg1Of Rbckl1,","
R347 T431 T438 arg1Of Rbckl1,result
R348 T433 T432 arg2Of Sipl1,","
R349 T433 T434 arg1Of Sipl1,)
R350 T433 T435 arg1Of Sipl1,gene
R351 T433 T436 arg1Of Sipl1,in
R352 T437 T436 arg2Of mice,in
R353 T438 T439 arg1Of result,in
R354 T441 T439 arg2Of inflammation,in
R355 T441 T440 arg1Of inflammation,systemic
R356 T441 T442 arg1Of inflammation,that
R357 T441 T443 arg1Of inflammation,is
R358 T441 T444 arg2Of inflammation,characterized
R359 T444 T443 arg2Of characterized,is
R360 T448 T446 arg1Of dermatitis,chronic
R361 T448 T447 arg1Of dermatitis,proliferative
R362 T448 T449 arg1Of dermatitis,and
R363 T449 T444 arg1Of and,characterized
R364 T449 T445 arg2Of and,by
R365 T451 T449 arg2Of secretion,and
R366 T451 T450 arg2Of secretion,dysregulated
R367 T451 T452 arg1Of secretion,of
R368 T454 T453 arg1Of helper1,T
R369 T454 T455 arg1Of helper1,(
R370 T454 T458 arg1Of helper1,and
R371 T456 T455 arg2Of Th1,(
R372 T457 T455 arg3Of ),(
R373 T459 T458 arg2Of Th2,and
R374 T460 T452 arg2Of cytokines,of
R375 T460 T454 arg1Of cytokines,helper1
R376 T460 T459 arg1Of cytokines,Th2
R377 T462 T463 arg1Of cellular,and
R378 T464 T463 arg2Of molecular,and
R379 T465 T461 arg1Of mechanisms,The
R380 T465 T462 arg1Of mechanisms,cellular
R381 T465 T464 arg1Of mechanisms,molecular
R382 T465 T466 arg1Of mechanisms,underlying
R383 T465 T470 arg1Of mechanisms,remain
R384 T465 T471 arg1Of mechanisms,elusive
R385 T469 T466 arg2Of phenotype,underlying
R386 T469 T467 arg1Of phenotype,this
R387 T469 T468 arg1Of phenotype,inflammatory
R388 T471 T470 arg2Of elusive,remain
R389 T473 T472 arg1Of cells,Dendritic
R390 T473 T474 arg1Of cells,may
R391 T473 T475 arg1Of cells,contribute
R392 T475 T474 arg2Of contribute,may
R393 T475 T476 arg1Of contribute,to
R394 T475 T487 arg1Of contribute,because
R395 T478 T479 arg1Of initiation,and
R396 T479 T476 arg2Of and,to
R397 T479 T477 arg1Of and,the
R398 T479 T481 arg1Of and,of
R399 T480 T479 arg2Of progression,and
R400 T483 T481 arg2Of phenotype,of
R401 T483 T482 arg1Of phenotype,the
R402 T483 T484 arg1Of phenotype,of
R403 T486 T484 arg2Of mice,of
R404 T486 T485 arg1Of mice,SHARPIN-deficient
R405 T487 T488 arg1Of because,of
R406 T491 T487 arg2Of role,because
R407 T491 T489 arg1Of role,their
R408 T491 T490 arg1Of role,pivotal
R409 T491 T492 arg1Of role,in
R410 T493 T494 arg1Of innate,and
R411 T495 T494 arg2Of adaptive,and
R412 T496 T492 arg2Of immunity,in
R413 T496 T493 arg1Of immunity,innate
R414 T496 T495 arg1Of immunity,adaptive
R415 T498 T499 arg1Of we,show
R416 T499 T497 arg1Of show,Here
R417 T499 T500 arg1Of show,by
R418 T502 T500 arg2Of cytometry,by
R419 T502 T501 arg1Of cytometry,flow
R420 T505 T504 arg1Of deficiency,SHARPIN-
R421 T505 T506 arg1Of deficiency,did
R422 T505 T508 arg1Of deficiency,alter
R423 T508 T499 arg2Of alter,show
R424 T508 T503 arg1Of alter,that
R425 T508 T506 arg2Of alter,did
R426 T508 T507 arg1Of alter,not
R427 T510 T508 arg2Of distribution,alter
R428 T510 T509 arg1Of distribution,the
R429 T510 T511 arg1Of distribution,of
R430 T514 T511 arg2Of subtypes,of
R431 T514 T512 arg1Of subtypes,different
R432 T514 T513 arg1Of subtypes,DC
R433 T514 T515 arg1Of subtypes,in
R434 T517 T515 arg2Of spleen,in
R435 T517 T516 arg1Of spleen,the
R436 T519 T518 arg2Of response,In
R437 T519 T520 arg1Of response,to
R438 T524 T523 arg2Of TLR,(
R439 T525 T523 arg3Of ),(
R440 T527 T521 arg1Of LPS,TOLL-like
R441 T527 T522 arg1Of LPS,receptor
R442 T527 T523 arg1Of LPS,(
R443 T527 T526 arg1Of LPS,agonists
R444 T527 T528 arg1Of LPS,and
R445 T528 T520 arg2Of and,to
R446 T530 T531 arg1Of I,:
R447 T531 T528 arg2Of :,and
R448 T531 T529 arg1Of :,poly
R449 T532 T531 arg2Of C,:
R450 T538 T534 arg2Of cells,cultured
R451 T538 T535 arg1Of cells,bone
R452 T538 T536 arg1Of cells,marrow-derived
R453 T538 T537 arg1Of cells,dendritic
R454 T538 T539 arg1Of cells,(
R455 T538 T542 arg1Of cells,from
R456 T538 T547 arg1Of cells,exhibited
R457 T540 T539 arg2Of BMDC,(
R458 T541 T539 arg3Of ),(
R459 T543 T544 arg1Of WT,and
R460 T545 T544 arg2Of mutant,and
R461 T546 T542 arg2Of mice,from
R462 T546 T543 arg1Of mice,WT
R463 T546 T545 arg1Of mice,mutant
R464 T547 T518 arg1Of exhibited,In
R465 T547 T533 arg1Of exhibited,","
R466 T549 T547 arg2Of increases,exhibited
R467 T549 T548 arg1Of increases,similar
R468 T549 T550 arg1Of increases,in
R469 T551 T550 arg2Of expression,in
R470 T551 T552 arg1Of expression,of
R471 T555 T556 arg1Of CD40,","
R472 T556 T559 arg1Of ",",and
R473 T557 T556 arg2Of CD80,","
R474 T559 T552 arg2Of and,of
R475 T559 T553 arg1Of and,co-stimulatory
R476 T559 T554 arg1Of and,molecules
R477 T559 T558 arg1Of and,","
R478 T560 T559 arg2Of CD86,and
R479 T565 T563 arg1Of BMDC,stimulated
R480 T565 T564 arg1Of BMDC,SHARPIN-deficient
R481 T565 T566 arg1Of BMDC,had
R482 T565 T567 arg1Of BMDC,reduced
R483 T567 T561 arg1Of reduced,However
R484 T567 T562 arg1Of reduced,","
R485 T567 T566 arg2Of reduced,had
R486 T568 T569 arg1Of transcription,and
R487 T569 T567 arg2Of and,reduced
R488 T569 T571 arg1Of and,of
R489 T570 T569 arg2Of secretion,and
R490 T573 T572 arg1Of mediators,pro-inflammatory
R491 T574 T575 arg1Of IL6,","
R492 T575 T577 arg1Of ",",","
R493 T576 T575 arg2Of IL12P70,","
R494 T577 T580 arg1Of ",",and
R495 T578 T577 arg2Of GMCSF,","
R496 T580 T571 arg2Of and,of
R497 T580 T573 arg1Of and,mediators
R498 T580 T579 arg1Of and,","
R499 T582 T580 arg2Of oxide,and
R500 T582 T581 arg1Of oxide,nitric
R501 T584 T583 arg1Of BMDC,Mutant
R502 T584 T585 arg1Of BMDC,had
R503 T585 T592 arg1Of had,whereas
R504 T587 T585 arg2Of activation,had
R505 T587 T586 arg1Of activation,defective
R506 T587 T588 arg1Of activation,of
R507 T590 T588 arg2Of signaling,of
R508 T590 T589 arg1Of signaling,NF-κB
R509 T592 T591 arg1Of whereas,","
R510 T594 T593 arg1Of MAPK1/3,the
R511 T594 T595 arg1Of MAPK1/3,(
R512 T594 T598 arg1Of MAPK1/3,and
R513 T596 T595 arg2Of ERK1/2,(
R514 T597 T595 arg3Of ),(
R515 T598 T610 arg1Of and,were
R516 T598 T611 arg1Of and,intact
R517 T599 T600 arg1Of MAPK11/12/13/14,(
R518 T599 T606 arg1Of MAPK11/12/13/14,and
R519 T604 T600 arg2Of isoforms,(
R520 T604 T601 arg1Of isoforms,p38
R521 T604 T602 arg1Of isoforms,MAP
R522 T604 T603 arg1Of isoforms,kinase
R523 T605 T600 arg3Of ),(
R524 T606 T598 arg2Of and,and
R525 T609 T606 arg2Of pathways,and
R526 T609 T607 arg1Of pathways,TBK1
R527 T609 T608 arg1Of pathways,signaling
R528 T610 T592 arg2Of were,whereas
R529 T611 T610 arg2Of intact,were
R530 T615 T612 arg1Of reaction,A
R531 T615 T613 arg1Of reaction,mixed
R532 T615 T614 arg1Of reaction,lymphocyte
R533 T615 T616 arg1Of reaction,showed
R534 T619 T618 arg1Of BMDC,mutant
R535 T619 T621 arg1Of BMDC,induced
R536 T619 T628 arg1Of BMDC,stimulated
R537 T621 T627 arg1Of induced,but
R538 T626 T621 arg2Of response,induced
R539 T626 T622 arg1Of response,a
R540 T626 T623 arg1Of response,weak
R541 T626 T624 arg1Of response,Th1
R542 T626 T625 arg1Of response,immune
R543 T627 T616 arg2Of but,showed
R544 T627 T617 arg1Of but,that
R545 T627 T620 arg1Of but,only
R546 T628 T627 arg2Of stimulated,but
R547 T628 T633 arg1Of stimulated,from
R548 T632 T628 arg2Of production,stimulated
R549 T632 T629 arg2Of production,increased
R550 T632 T630 arg1Of production,Th2
R551 T632 T631 arg1Of production,cytokine
R552 T638 T633 arg2Of cells,from
R553 T638 T634 arg1Of cells,allogeneic
R554 T638 T635 arg1Of cells,naïve
R555 T638 T636 arg1Of cells,CD4+
R556 T638 T637 arg1Of cells,T
R557 T640 T639 arg2Of conclusion,In
R558 T642 T643 arg1Of loss,of
R559 T642 T645 arg1Of loss,in
R560 T642 T648 arg1Of loss,affects
R561 T644 T643 arg2Of Sharpin,of
R562 T646 T645 arg2Of mice,in
R563 T648 T647 arg1Of affects,significantly
R564 T648 T654 arg1Of affects,and
R565 T651 T648 arg2Of function,affects
R566 T651 T649 arg1Of function,the
R567 T651 T650 arg1Of function,immune
R568 T651 T652 arg1Of function,of
R569 T653 T652 arg2Of DC,of
R570 T654 T639 arg1Of and,In
R571 T654 T641 arg1Of and,","
R572 T655 T656 arg1Of this,may
R573 T655 T658 arg1Of this,account
R574 T658 T654 arg2Of account,and
R575 T658 T656 arg2Of account,may
R576 T658 T657 arg1Of account,partially
R577 T658 T659 arg1Of account,for
R578 T662 T661 arg1Of inflammation,systemic
R579 T662 T663 arg1Of inflammation,and
R580 T663 T659 arg2Of and,for
R581 T663 T660 arg1Of and,the
R582 T666 T663 arg2Of response,and
R583 T666 T664 arg1Of response,Th2-biased
R584 T666 T665 arg1Of response,immune
R1248 T1486 T1487 arg1Of SHARPIN,was
R1249 T1486 T1489 arg2Of SHARPIN,identified
R1250 T1489 T1487 arg2Of identified,was
R1251 T1489 T1488 arg1Of identified,originally
R1252 T1489 T1490 arg1Of identified,in
R1253 T1489 T1505 arg1Of identified,but
R1254 T1492 T1490 arg2Of densities,in
R1255 T1492 T1491 arg1Of densities,post-synaptic
R1256 T1492 T1493 arg1Of densities,of
R1257 T1495 T1493 arg2Of synapses,of
R1258 T1495 T1494 arg1Of synapses,excitatory
R1259 T1495 T1496 arg1Of synapses,in
R1260 T1498 T1496 arg2Of brain,in
R1261 T1498 T1497 arg1Of brain,the
R1262 T1498 T1499 arg1Of brain,of
R1263 T1500 T1499 arg2Of rats,of
R1264 T1500 T1501 arg1Of rats,[
R1265 T1502 T1501 arg2Of 1,[
R1266 T1503 T1501 arg3Of ],[
R1267 T1505 T1504 arg1Of but,","
R1268 T1507 T1506 arg1Of protein,this
R1269 T1507 T1508 arg1Of protein,is
R1270 T1507 T1510 arg2Of protein,expressed
R1271 T1510 T1505 arg2Of expressed,but
R1272 T1510 T1508 arg2Of expressed,is
R1273 T1510 T1509 arg1Of expressed,widely
R1274 T1510 T1511 arg1Of expressed,in
R1275 T1513 T1511 arg2Of variety,in
R1276 T1513 T1512 arg1Of variety,a
R1277 T1513 T1514 arg1Of variety,of
R1278 T1515 T1514 arg2Of tissues,of
R1279 T1515 T1516 arg1Of tissues,[
R1280 T1517 T1516 arg2Of 2,[
R1281 T1518 T1516 arg3Of ],[
R1282 T1520 T1521 arg1Of allelic,","
R1283 T1522 T1521 arg2Of autosomal,","
R1284 T1524 T1519 arg1Of mutations,Two
R1285 T1524 T1520 arg1Of mutations,allelic
R1286 T1524 T1522 arg1Of mutations,autosomal
R1287 T1524 T1523 arg1Of mutations,recessive
R1288 T1524 T1525 arg1Of mutations,in
R1289 T1524 T1529 arg1Of mutations,occurred
R1290 T1528 T1525 arg2Of gene,in
R1291 T1528 T1526 arg1Of gene,the
R1292 T1528 T1527 arg1Of gene,Sharpin
R1293 T1529 T1530 arg1Of occurred,spontaneously
R1294 T1529 T1531 arg1Of occurred,in
R1295 T1529 T1541 arg1Of occurred,","
R1296 T1529 T1542 modOf occurred,resulting
R1297 T1529 T1556 arg1Of occurred,[
R1298 T1534 T1531 arg2Of strains,in
R1299 T1534 T1532 arg1Of strains,two
R1300 T1534 T1533 arg1Of strains,inbred
R1301 T1534 T1535 arg1Of strains,of
R1302 T1534 T1537 arg1Of strains,","
R1303 T1536 T1535 arg2Of mice,of
R1304 T1538 T1539 arg1Of C57B/KaLawRij-Sharpincpdm/Sharpincpdm,and
R1305 T1539 T1537 arg2Of and,","
R1306 T1540 T1539 arg2Of CBy.Ocb3/Dem-Sharpincpdm-Dem/Sharpincpdm-Dem,and
R1307 T1542 T1543 arg1Of resulting,in
R1308 T1545 T1544 arg1Of termination,premature
R1309 T1545 T1546 arg1Of termination,of
R1310 T1545 T1549 arg1Of termination,and
R1311 T1548 T1546 arg2Of synthesis,of
R1312 T1548 T1547 arg1Of synthesis,mRNA
R1313 T1549 T1543 arg2Of and,in
R1314 T1550 T1549 arg2Of absence,and
R1315 T1550 T1551 arg1Of absence,of
R1316 T1555 T1551 arg2Of product,of
R1317 T1555 T1552 arg1Of product,a
R1318 T1555 T1553 arg1Of product,functional
R1319 T1555 T1554 arg1Of product,protein
R1320 T1557 T1556 arg2Of 2,[
R1321 T1558 T1556 arg3Of ],[
R1322 T1562 T1559 arg2Of backgrounds,Despite
R1323 T1562 T1560 arg1Of backgrounds,different
R1324 T1562 T1561 arg1Of backgrounds,genetic
R1325 T1565 T1564 arg1Of mutations,both
R1326 T1565 T1566 arg1Of mutations,cause
R1327 T1566 T1559 arg1Of cause,Despite
R1328 T1566 T1563 arg1Of cause,","
R1329 T1566 T1586 arg1Of cause,[
R1330 T1569 T1566 arg2Of disease,cause
R1331 T1569 T1567 arg1Of disease,similar
R1332 T1569 T1568 arg1Of disease,inflammatory
R1333 T1569 T1570 arg1Of disease,with
R1334 T1573 T1574 arg1Of progressive,dermatitis
R1335 T1573 T1575 arg1Of progressive,and
R1336 T1576 T1575 arg2Of defective,and
R1337 T1577 T1570 arg2Of development,with
R1338 T1577 T1571 arg1Of development,severe
R1339 T1577 T1572 arg1Of development,chronic
R1340 T1577 T1573 arg1Of development,progressive
R1341 T1577 T1576 arg1Of development,defective
R1342 T1577 T1578 arg1Of development,of
R1343 T1577 T1585 arg1Of development,–
R1344 T1581 T1578 arg2Of organs,of
R1345 T1581 T1579 arg1Of organs,secondary
R1346 T1581 T1580 arg1Of organs,lymphoid
R1347 T1581 T1582 arg1Of organs,[
R1348 T1583 T1582 arg2Of 2,[
R1349 T1584 T1582 arg3Of ],[
R1350 T1587 T1586 arg2Of 4,[
R1351 T1588 T1586 arg3Of ],[
R1352 T1590 T1589 arg1Of dermatitis,The
R1353 T1590 T1591 arg1Of dermatitis,becomes
R1354 T1590 T1593 arg1Of dermatitis,apparent
R1355 T1591 T1594 arg1Of becomes,at
R1356 T1593 T1591 arg2Of apparent,becomes
R1357 T1593 T1592 arg1Of apparent,clinically
R1358 T1596 T1595 arg1Of four,about
R1359 T1597 T1594 arg2Of weeks,at
R1360 T1597 T1596 arg1Of weeks,four
R1361 T1597 T1598 arg1Of weeks,of
R1362 T1599 T1598 arg2Of age,of
R1363 T1600 T1601 arg1Of There,are
R1364 T1602 T1601 arg2Of accumulations,are
R1365 T1602 T1603 arg1Of accumulations,of
R1366 T1602 T1616 arg1Of accumulations,(
R1367 T1602 T1624 arg2Of accumulations,associated
R1368 T1604 T1605 arg1Of eosinophils,","
R1369 T1605 T1607 arg1Of ",",and
R1370 T1606 T1605 arg2Of neutrophils,","
R1371 T1607 T1603 arg2Of and,of
R1372 T1607 T1609 arg1Of and,in
R1373 T1608 T1607 arg2Of macrophages,and
R1374 T1611 T1609 arg2Of skin,in
R1375 T1611 T1610 arg1Of skin,the
R1376 T1611 T1612 arg1Of skin,of
R1377 T1615 T1612 arg2Of mice,of
R1378 T1615 T1613 arg1Of mice,Sharpincpdm/Sharpincpdm
R1379 T1615 T1614 arg1Of mice,mutant
R1380 T1618 T1616 arg2Of referred,(
R1381 T1618 T1617 arg1Of referred,hereafter
R1382 T1618 T1619 arg1Of referred,to
R1383 T1618 T1620 arg1Of referred,as
R1384 T1622 T1620 arg2Of mice,as
R1385 T1622 T1621 arg1Of mice,cpdm
R1386 T1623 T1616 arg3Of ),(
R1387 T1624 T1625 arg1Of associated,with
R1388 T1624 T1635 arg1Of associated,in
R1389 T1625 T1634 arg1Of with,and
R1390 T1627 T1625 arg2Of expression,with
R1391 T1627 T1626 arg2Of expression,increased
R1392 T1627 T1628 arg1Of expression,of
R1393 T1627 T1631 arg1Of expression,in
R1394 T1630 T1628 arg2Of cytokines,of
R1395 T1630 T1629 arg1Of cytokines,Th2
R1396 T1633 T1631 arg2Of skin,in
R1397 T1633 T1632 arg1Of skin,the
R1398 T1635 T1634 arg2Of in,and
R1399 T1637 T1635 arg2Of supernatants,in
R1400 T1637 T1636 arg1Of supernatants,the
R1401 T1637 T1638 arg1Of supernatants,of
R1402 T1640 T1638 arg2Of splenocytes,of
R1403 T1640 T1639 arg2Of splenocytes,activated
R1404 T1640 T1641 arg1Of splenocytes,[
R1405 T1642 T1641 arg2Of 5,[
R1406 T1643 T1641 arg3Of ],[
R1407 T1645 T1644 arg1Of mice,The
R1408 T1645 T1646 arg1Of mice,have
R1409 T1645 T1654 arg1Of mice,decreased
R1410 T1646 T1653 arg1Of have,and
R1411 T1652 T1646 arg2Of response,have
R1412 T1652 T1647 arg1Of response,an
R1413 T1652 T1648 arg1Of response,impaired
R1414 T1652 T1649 arg2Of response,delayed
R1415 T1652 T1650 arg1Of response,type
R1416 T1652 T1651 arg1Of response,hypersensitivity
R1417 T1653 T1658 arg1Of and,[
R1418 T1653 T1661 arg1Of and,","
R1419 T1653 T1662 modOf and,indicating
R1420 T1654 T1653 arg2Of decreased,and
R1421 T1655 T1654 arg2Of secretion,decreased
R1422 T1655 T1656 arg1Of secretion,of
R1423 T1657 T1656 arg2Of IFNγ,of
R1424 T1659 T1658 arg2Of 5,[
R1425 T1660 T1658 arg3Of ],[
R1426 T1664 T1663 arg1Of defect,a
R1427 T1664 T1665 arg1Of defect,in
R1428 T1664 T1669 arg1Of defect,and
R1429 T1668 T1665 arg2Of responses,in
R1430 T1668 T1666 arg1Of responses,Th1
R1431 T1668 T1667 arg1Of responses,immune
R1432 T1669 T1662 arg2Of and,indicating
R1433 T1671 T1669 arg2Of bias,and
R1434 T1671 T1670 arg1Of bias,a
R1435 T1671 T1672 arg1Of bias,towards
R1436 T1676 T1672 arg2Of response,towards
R1437 T1676 T1673 arg1Of response,a
R1438 T1676 T1674 arg1Of response,Th2
R1439 T1676 T1675 arg1Of response,immune
R1440 T1678 T1677 arg1Of treatment,Systemic
R1441 T1678 T1679 arg1Of treatment,of
R1442 T1678 T1682 arg1Of treatment,with
R1443 T1678 T1685 arg1Of treatment,caused
R1444 T1681 T1679 arg2Of mice,of
R1445 T1681 T1680 arg1Of mice,cpdm
R1446 T1684 T1682 arg2Of IL12,with
R1447 T1684 T1683 arg1Of IL12,recombinant
R1448 T1685 T1691 arg1Of caused,[
R1449 T1687 T1685 arg2Of remission,caused
R1450 T1687 T1686 arg1Of remission,complete
R1451 T1687 T1688 arg1Of remission,of
R1452 T1690 T1688 arg2Of dermatitis,of
R1453 T1690 T1689 arg1Of dermatitis,the
R1454 T1692 T1691 arg2Of 5,[
R1455 T1693 T1691 arg3Of ],[
R1456 T1694 T1695 arg1Of Neutralization,of
R1457 T1694 T1697 arg1Of Neutralization,by
R1458 T1694 T1705 arg1Of Neutralization,reduced
R1459 T1694 T1715 arg1Of Neutralization,failed
R1460 T1694 T1717 arg1Of Neutralization,reduce
R1461 T1696 T1695 arg2Of IL5,of
R1462 T1699 T1698 arg1Of treatment,antibody
R1463 T1699 T1700 arg1Of treatment,or
R1464 T1700 T1697 arg2Of or,by
R1465 T1701 T1700 arg2Of crosses,or
R1466 T1701 T1702 arg1Of crosses,with
R1467 T1704 T1702 arg2Of mice,with
R1468 T1704 T1703 arg1Of mice,IL5-deficient
R1469 T1705 T1714 arg1Of reduced,but
R1470 T1707 T1705 arg2Of number,reduced
R1471 T1707 T1706 arg1Of number,the
R1472 T1707 T1708 arg1Of number,of
R1473 T1712 T1708 arg2Of eosinophils,of
R1474 T1712 T1709 arg1Of eosinophils,circulating
R1475 T1712 T1710 arg1Of eosinophils,and
R1476 T1712 T1711 arg1Of eosinophils,cutaneous
R1477 T1714 T1713 arg1Of but,","
R1478 T1715 T1714 arg2Of failed,but
R1479 T1717 T1715 arg2Of reduce,failed
R1480 T1717 T1716 arg1Of reduce,to
R1481 T1717 T1725 arg1Of reduce,[
R1482 T1719 T1720 arg1Of onset,and
R1483 T1720 T1717 arg2Of and,reduce
R1484 T1720 T1718 arg1Of and,the
R1485 T1720 T1722 arg1Of and,of
R1486 T1721 T1720 arg2Of severity,and
R1487 T1724 T1722 arg2Of dermatitis,of
R1488 T1724 T1723 arg1Of dermatitis,the
R1489 T1726 T1725 arg2Of 6,[
R1490 T1727 T1725 arg3Of ],[
R1491 T1732 T1730 arg1Of groups,three
R1492 T1732 T1731 arg1Of groups,independent
R1493 T1732 T1733 arg1Of groups,identified
R1494 T1733 T1728 arg1Of identified,Recently
R1495 T1733 T1729 arg1Of identified,","
R1496 T1733 T1735 arg1Of identified,as
R1497 T1734 T1733 arg2Of SHARPIN,identified
R1498 T1738 T1735 arg2Of component,as
R1499 T1738 T1736 arg1Of component,an
R1500 T1738 T1737 arg1Of component,essential
R1501 T1738 T1739 arg1Of component,of
R1502 T1745 T1739 arg2Of complex,of
R1503 T1745 T1740 arg1Of complex,the
R1504 T1745 T1741 arg1Of complex,linear
R1505 T1745 T1742 arg1Of complex,ubiquitin
R1506 T1745 T1743 arg1Of complex,chain
R1507 T1745 T1744 arg1Of complex,assembly
R1508 T1745 T1746 arg1Of complex,(
R1509 T1745 T1749 arg1Of complex,that
R1510 T1745 T1750 arg1Of complex,regulates
R1511 T1747 T1746 arg2Of LUBAC,(
R1512 T1748 T1746 arg3Of ),(
R1513 T1756 T1755 arg2Of 7,[
R1514 T1757 T1755 arg3Of ],[
R1515 T1758 T1750 arg2Of –,regulates
R1516 T1758 T1751 arg1Of –,TNFα-induced
R1517 T1758 T1752 arg1Of –,canonical
R1518 T1758 T1753 arg1Of –,NF-κB
R1519 T1758 T1754 arg1Of –,signaling
R1520 T1758 T1755 arg1Of –,[
R1521 T1758 T1759 arg1Of –,[
R1522 T1760 T1759 arg2Of 9,[
R1523 T1761 T1759 arg3Of ],[
R1524 T1765 T1762 arg1Of fibroblast,SHARPIN-deficient
R1525 T1765 T1763 arg1Of fibroblast,mouse
R1526 T1765 T1764 arg1Of fibroblast,embryonic
R1527 T1765 T1766 arg1Of fibroblast,(
R1528 T1765 T1769 arg1Of fibroblast,were
R1529 T1765 T1770 arg2Of fibroblast,sensitized
R1530 T1767 T1766 arg2Of MEF,(
R1531 T1768 T1766 arg3Of ),(
R1532 T1770 T1769 arg2Of sensitized,were
R1533 T1770 T1771 arg1Of sensitized,to
R1534 T1770 T1774 arg1Of sensitized,and
R1535 T1773 T1771 arg2Of apoptosis,to
R1536 T1773 T1772 arg1Of apoptosis,TNFα-induced
R1537 T1776 T1775 arg1Of death,cell
R1538 T1776 T1777 arg1Of death,was
R1539 T1776 T1778 arg2Of death,implicated
R1540 T1778 T1774 arg2Of implicated,and
R1541 T1778 T1777 arg2Of implicated,was
R1542 T1778 T1779 arg1Of implicated,as
R1543 T1778 T1792 arg1Of implicated,[
R1544 T1781 T1779 arg2Of factor,as
R1545 T1781 T1780 arg1Of factor,a
R1546 T1781 T1782 arg1Of factor,in
R1547 T1784 T1783 arg1Of dermatitis,the
R1548 T1784 T1785 arg1Of dermatitis,of
R1549 T1784 T1788 arg1Of dermatitis,[
R1550 T1787 T1785 arg2Of mice,of
R1551 T1787 T1786 arg1Of mice,cpdm
R1552 T1789 T1788 arg2Of 7,[
R1553 T1790 T1788 arg3Of ],[
R1554 T1791 T1782 arg2Of –,in
R1555 T1791 T1784 arg1Of –,dermatitis
R1556 T1793 T1792 arg2Of 9,[
R1557 T1794 T1792 arg3Of ],[
R1558 T1796 T1795 arg1Of cells,Dendritic
R1559 T1796 T1797 arg1Of cells,(
R1560 T1796 T1800 arg1Of cells,have
R1561 T1798 T1797 arg2Of DC,(
R1562 T1799 T1797 arg3Of ),(
R1563 T1800 T1804 arg1Of have,in
R1564 T1803 T1800 arg2Of role,have
R1565 T1803 T1801 arg1Of role,a
R1566 T1803 T1802 arg1Of role,sentinel
R1567 T1805 T1804 arg2Of sensing,in
R1568 T1806 T1807 arg1Of pathogen,or
R1569 T1808 T1807 arg2Of danger,or
R1570 T1809 T1810 arg1Of signals,and
R1571 T1810 T1806 arg1Of and,pathogen
R1572 T1810 T1808 arg1Of and,danger
R1573 T1810 T1812 arg1Of and,and
R1574 T1811 T1810 arg2Of initiate,and
R1575 T1812 T1805 arg2Of and,sensing
R1576 T1814 T1812 arg2Of activation,and
R1577 T1814 T1813 arg1Of activation,direct
R1578 T1814 T1815 arg1Of activation,of
R1579 T1819 T1815 arg2Of response,of
R1580 T1819 T1816 arg1Of response,the
R1581 T1819 T1817 arg1Of response,adaptive
R1582 T1819 T1818 arg1Of response,immune
R1583 T1819 T1820 arg1Of response,[
R1584 T1821 T1820 arg2Of 10,[
R1585 T1822 T1820 arg3Of ],[
R1586 T1823 T1824 arg1Of Activated,and
R1587 T1825 T1824 arg2Of mature,and
R1588 T1826 T1823 arg1Of DC,Activated
R1589 T1826 T1825 arg1Of DC,mature
R1590 T1826 T1827 arg1Of DC,can
R1591 T1826 T1828 arg1Of DC,carry
R1592 T1826 T1833 arg1Of DC,migrate
R1593 T1826 T1839 arg1Of DC,induce
R1594 T1828 T1827 arg2Of carry,can
R1595 T1828 T1832 arg1Of carry,","
R1596 T1831 T1828 arg2Of peptides,carry
R1597 T1831 T1829 arg2Of peptides,processed
R1598 T1831 T1830 arg1Of peptides,antigenic
R1599 T1832 T1838 arg1Of ",",and
R1600 T1833 T1832 arg2Of migrate,","
R1601 T1833 T1834 arg1Of migrate,to
R1602 T1836 T1834 arg2Of organs,to
R1603 T1836 T1835 arg1Of organs,lymphoid
R1604 T1838 T1837 arg1Of and,","
R1605 T1839 T1838 arg2Of induce,and
R1606 T1842 T1840 arg1Of responses,T-cell-mediated
R1607 T1842 T1841 arg1Of responses,immune
R1608 T1842 T1843 arg1Of responses,or
R1609 T1843 T1839 arg2Of or,induce
R1610 T1844 T1843 arg2Of tolerance,or
R1611 T1845 T1846 arg1Of DC,direct
R1612 T1846 T1861 arg1Of direct,","
R1613 T1846 T1862 arg1Of direct,through
R1614 T1848 T1847 arg1Of differentiation,the
R1615 T1848 T1849 arg1Of differentiation,of
R1616 T1848 T1854 arg1Of differentiation,and
R1617 T1852 T1849 arg2Of cells,of
R1618 T1852 T1850 arg1Of cells,CD4+
R1619 T1852 T1851 arg1Of cells,T
R1620 T1854 T1846 arg2Of and,direct
R1621 T1854 T1853 arg1Of and,","
R1622 T1857 T1854 arg2Of type,and
R1623 T1857 T1855 arg1Of type,hence
R1624 T1857 T1856 arg1Of type,the
R1625 T1857 T1858 arg1Of type,of
R1626 T1860 T1858 arg2Of response,of
R1627 T1860 T1859 arg1Of response,immune
R1628 T1865 T1862 arg2Of secretion,through
R1629 T1865 T1863 arg1Of secretion,the
R1630 T1865 T1864 arg1Of secretion,selective
R1631 T1865 T1866 arg1Of secretion,of
R1632 T1867 T1866 arg2Of cytokines,of
R1633 T1868 T1869 arg1Of We,hypothesized
R1634 T1873 T1871 arg1Of secretion,defective
R1635 T1873 T1872 arg1Of secretion,cytokine
R1636 T1873 T1874 arg1Of secretion,by
R1637 T1873 T1876 arg1Of secretion,contributed
R1638 T1875 T1874 arg2Of DC,by
R1639 T1876 T1869 arg2Of contributed,hypothesized
R1640 T1876 T1870 arg1Of contributed,that
R1641 T1902 T1900 arg2Of mediators,of
R1642 T1902 T1901 arg1Of mediators,pro-inflammatory
R1643 T1876 T1877 arg1Of contributed,to
R1644 T1903 T1897 arg2Of and,caused
R1645 T1881 T1877 arg2Of phenotype,to
R1646 T1906 T1903 arg2Of activation,and
R1647 T1906 T1904 arg1Of activation,impaired
R1648 T1906 T1905 arg1Of activation,NF-κB
R1649 T1906 T1907 arg1Of activation,upon
R1650 T1881 T1878 arg1Of phenotype,the
R1651 T1881 T1879 arg1Of phenotype,Th2-biased
R1652 T1881 T1880 arg1Of phenotype,inflammatory
R1653 T1909 T1907 arg2Of stimulation,upon
R1654 T1909 T1908 arg1Of stimulation,ligand
R1655 T1881 T1882 arg1Of phenotype,in
R1656 T1884 T1882 arg2Of mice,in
R1657 T1911 T1910 arg1Of ability,The
R1658 T1911 T1912 arg1Of ability,of
R1659 T1911 T1915 modOf ability,to
R1660 T1911 T1925 arg1Of ability,was
R1661 T1911 T1926 arg2Of ability,compromised
R1662 T1914 T1912 arg2Of BMDC,of
R1663 T1914 T1913 arg1Of BMDC,cpdm
R1664 T1916 T1915 arg1Of stimulate,to
R1665 T1884 T1883 arg1Of mice,SHARPIN-deficient
R1666 T1886 T1885 arg1Of studies,The
R1667 T1919 T1916 arg2Of production,stimulate
R1668 T1919 T1917 arg1Of production,Th1
R1669 T1919 T1918 arg1Of production,cytokine
R1670 T1919 T1920 arg1Of production,in
R1671 T1886 T1887 arg2Of studies,reported
R1672 T1886 T1889 arg1Of studies,found
R1673 T1887 T1888 arg1Of reported,here
R1674 T1924 T1920 arg2Of cells,in
R1675 T1924 T1921 arg1Of cells,allogeneic
R1676 T1924 T1922 arg1Of cells,CD4+
R1677 T1924 T1923 arg1Of cells,T
R1678 T1926 T1925 arg2Of compromised,was
R1679 T1927 T1928 arg1Of Taken,together
R1680 T1891 T1892 arg1Of lack,of
R1681 T1931 T1930 arg1Of results,these
R1682 T1931 T1932 arg1Of results,reveal
R1683 T1931 T1944 arg1Of results,suggest
R1684 T1932 T1943 arg1Of reveal,and
R1685 T1934 T1935 arg1Of SHARPIN,is
R1686 T1935 T1932 arg2Of is,reveal
R1687 T1935 T1933 arg1Of is,that
R1688 T1935 T1940 arg1Of is,in
R1689 T1891 T1897 arg1Of lack,caused
R1690 T1894 T1892 arg2Of protein,of
R1691 T1939 T1935 arg2Of molecule,is
R1692 T1939 T1936 arg1Of molecule,a
R1693 T1939 T1937 arg1Of molecule,novel
R1694 T1939 T1938 arg1Of molecule,regulatory
R1695 T1942 T1940 arg2Of biology,in
R1696 T1942 T1941 arg1Of biology,DC
R1697 T1943 T1927 modOf and,Taken
R1698 T1943 T1929 arg1Of and,","
R1699 T1944 T1943 arg2Of suggest,and
R1700 T1894 T1893 arg1Of protein,SHARPIN
R1701 T1894 T1895 arg1Of protein,in
R1702 T1896 T1895 arg2Of BMDC,in
R1703 T1948 T1946 arg1Of function,the
R1704 T1948 T1947 arg2Of function,dysregulated
R1705 T1948 T1949 arg1Of function,of
R1706 T1948 T1952 arg1Of function,plays
R1707 T1897 T1889 arg2Of caused,found
R1708 T1951 T1949 arg2Of DC,of
R1709 T1951 T1950 arg1Of DC,SHARPIN-deficient
R1710 T1952 T1944 arg2Of plays,suggest
R1711 T1952 T1945 arg1Of plays,that
R1712 T1952 T1955 arg1Of plays,in
R1713 T1897 T1890 arg1Of caused,that
R1714 T1954 T1952 arg2Of role,plays
R1715 T1954 T1953 arg1Of role,a
R1716 T1899 T1898 arg1Of expression,defective
R1717 T1958 T1955 arg2Of phenotype,in
R1718 T1958 T1956 arg1Of phenotype,the
R1719 T1958 T1957 arg1Of phenotype,cpdm
R1720 T1899 T1900 arg1Of expression,of
R1721 T1899 T1903 arg1Of expression,and
R2041 T2461 T2462 arg1Of Characterization,of
R2042 T2463 T2462 arg2Of Sharpin,of
R2043 T2464 T2465 arg1Of Orthologs,of
R2044 T2464 T2468 arg1Of Orthologs,are
R2045 T2464 T2469 arg2Of Orthologs,found
R2046 T2467 T2465 arg2Of protein,of
R2047 T2467 T2466 arg1Of protein,SHARPIN
R2048 T2469 T2468 arg2Of found,are
R2049 T2469 T2470 arg1Of found,in
R2050 T2472 T2470 arg2Of species,in
R2051 T2472 T2471 arg1Of species,various
R2052 T2472 T2473 arg1Of species,","
R2053 T2472 T2474 arg1Of species,including
R2054 T2475 T2476 arg1Of human,","
R2055 T2477 T2478 arg1Of mouse,and
R2056 T2478 T2474 arg2Of and,including
R2057 T2478 T2475 arg1Of and,human
R2058 T2479 T2478 arg2Of rat,and
R2059 T2482 T2480 arg1Of programming,Motif
R2060 T2482 T2481 arg1Of programming,prediction
R2061 T2482 T2495 arg1Of programming,suggests
R2062 T2485 T2486 arg1Of COILS,[
R2063 T2485 T2489 arg1Of COILS,and
R2064 T2487 T2486 arg2Of 11,[
R2065 T2488 T2486 arg3Of ],[
R2066 T2489 T2484 arg2Of and,using
R2067 T2490 T2489 arg2Of MotifScan,and
R2068 T2490 T2491 arg1Of MotifScan,[
R2069 T2492 T2491 arg2Of 12,[
R2070 T2493 T2491 arg3Of ],[
R2071 T2495 T2483 arg1Of suggests,","
R2072 T2495 T2484 modOf suggests,using
R2073 T2495 T2494 arg1Of suggests,","
R2074 T2497 T2498 arg1Of SHARPIN,contains
R2075 T2498 T2495 arg2Of contains,suggests
R2076 T2498 T2496 arg1Of contains,that
R2077 T2502 T2501 arg2Of CC,(
R2078 T2503 T2501 arg3Of ),(
R2079 T2504 T2499 arg1Of domain,a
R2080 T2504 T2500 arg1Of domain,coiled-coil
R2081 T2504 T2501 arg1Of domain,(
R2082 T2504 T2505 arg1Of domain,","
R2083 T2504 T2513 arg1Of domain,and
R2084 T2507 T2508 arg1Of ubiquitin-like,(
R2085 T2509 T2508 arg2Of UBL,(
R2086 T2510 T2508 arg3Of ),(
R2087 T2511 T2505 arg2Of domain,","
R2088 T2511 T2506 arg1Of domain,a
R2089 T2511 T2507 arg1Of domain,ubiquitin-like
R2090 T2513 T2498 arg2Of and,contains
R2091 T2513 T2512 arg1Of and,","
R2092 T2520 T2519 arg2Of ZFRBP,(
R2093 T2521 T2519 arg3Of ),(
R2094 T2522 T2513 arg2Of domain,and
R2095 T2522 T2514 arg1Of domain,a
R2096 T2522 T2515 arg1Of domain,zinc-finger
R2097 T2522 T2516 arg1Of domain,Ran-binding
R2098 T2522 T2517 arg1Of domain,protein
R2099 T2522 T2518 arg1Of domain,2
R2100 T2522 T2519 arg1Of domain,(
R2101 T2525 T2523 arg1Of motifs,These
R2102 T2525 T2524 arg1Of motifs,functional
R2103 T2525 T2526 arg1Of motifs,constitute
R2104 T2526 T2545 arg1Of constitute,","
R2105 T2526 T2546 modOf constitute,suggesting
R2106 T2529 T2526 arg2Of profiles,constitute
R2107 T2529 T2527 arg1Of profiles,similar
R2108 T2529 T2528 arg1Of profiles,domain
R2109 T2529 T2530 arg1Of profiles,that
R2110 T2529 T2531 arg1Of profiles,are
R2111 T2529 T2532 arg1Of profiles,present
R2112 T2532 T2531 arg2Of present,are
R2113 T2532 T2533 arg1Of present,in
R2114 T2536 T2533 arg2Of protein,in
R2115 T2536 T2534 arg1Of protein,the
R2116 T2536 T2535 arg1Of protein,SHARPIN
R2117 T2536 T2537 arg1Of protein,of
R2118 T2540 T2537 arg2Of origins,of
R2119 T2540 T2538 arg1Of origins,all
R2120 T2540 T2539 arg1Of origins,three
R2121 T2540 T2541 arg1Of origins,(
R2122 T2543 T2541 arg2Of 1A,(
R2123 T2543 T2542 arg1Of 1A,Fig.
R2124 T2544 T2541 arg3Of ),(
R2125 T2546 T2553 arg1Of suggesting,across
R2126 T2548 T2549 arg1Of SHARPIN,exerts
R2127 T2549 T2546 arg2Of exerts,suggesting
R2128 T2549 T2547 arg1Of exerts,that
R2129 T2552 T2549 arg2Of functions,exerts
R2130 T2552 T2550 arg1Of functions,highly
R2131 T2552 T2551 arg2Of functions,conserved
R2132 T2554 T2553 arg2Of species,across
R2133 T2556 T2555 arg1Of mutations,Spontaneous
R2134 T2556 T2557 arg1Of mutations,in
R2135 T2556 T2562 arg1Of mutations,results
R2136 T2561 T2557 arg2Of gene,in
R2137 T2561 T2558 arg1Of gene,the
R2138 T2561 T2559 arg1Of gene,mouse
R2139 T2561 T2560 arg1Of gene,Sharpin
R2140 T2562 T2563 arg1Of results,in
R2141 T2566 T2565 arg1Of inflammatory,complex
R2142 T2567 T2563 arg2Of phenotype,in
R2143 T2567 T2564 arg1Of phenotype,a
R2144 T2567 T2566 arg1Of phenotype,inflammatory
R2145 T2567 T2568 arg2Of phenotype,characterized
R2146 T2571 T2570 arg1Of dermatitis,severe
R2147 T2571 T2572 arg1Of dermatitis,(
R2148 T2571 T2576 arg1Of dermatitis,","
R2149 T2574 T2572 arg2Of 1B,(
R2150 T2574 T2573 arg1Of 1B,Fig.
R2151 T2575 T2572 arg3Of ),(
R2152 T2576 T2579 arg1Of ",",and
R2153 T2578 T2576 arg2Of inflammation,","
R2154 T2578 T2577 arg1Of inflammation,systemic
R2155 T2579 T2568 arg1Of and,characterized
R2156 T2579 T2569 arg2Of and,by
R2157 T2582 T2579 arg2Of spleen,and
R2158 T2582 T2580 arg1Of spleen,an
R2159 T2582 T2581 arg1Of spleen,enlarged
R2160 T2582 T2583 arg1Of spleen,(
R2161 T2582 T2587 arg2Of spleen,caused
R2162 T2585 T2583 arg2Of 1C,(
R2163 T2585 T2584 arg1Of 1C,Fig.
R2164 T2586 T2583 arg3Of ),(
R2165 T2590 T2587 arg1Of hematopoiesis,caused
R2166 T2590 T2588 arg2Of hematopoiesis,by
R2167 T2590 T2589 arg1Of hematopoiesis,extramedullary
R2168 T2590 T2591 arg1Of hematopoiesis,[
R2169 T2592 T2591 arg2Of 3,[
R2170 T2593 T2591 arg3Of ],[
R2171 T2596 T2594 arg1Of expression,The
R2172 T2596 T2595 arg1Of expression,endogenous
R2173 T2596 T2597 arg1Of expression,of
R2174 T2596 T2600 arg1Of expression,in
R2175 T2596 T2602 arg1Of expression,was
R2176 T2596 T2603 arg2Of expression,determined
R2177 T2599 T2597 arg2Of mRNA,of
R2178 T2599 T2598 arg1Of mRNA,Sharpin
R2179 T2601 T2600 arg2Of BMDC,in
R2180 T2603 T2602 arg2Of determined,was
R2181 T2603 T2611 arg1Of determined,following
R2182 T2603 T2613 arg1Of determined,in
R2183 T2607 T2603 arg1Of time-PCR,determined
R2184 T2607 T2604 arg2Of time-PCR,by
R2185 T2607 T2605 arg1Of time-PCR,quantitative
R2186 T2607 T2606 arg1Of time-PCR,real
R2187 T2607 T2608 arg1Of time-PCR,(
R2188 T2609 T2608 arg2Of qRT-PCR,(
R2189 T2610 T2608 arg3Of ),(
R2190 T2612 T2611 arg2Of culture,following
R2191 T2614 T2613 arg2Of medium,in
R2192 T2615 T2616 arg1Of only,or
R2193 T2616 T2613 arg3Of or,in
R2194 T2617 T2616 arg2Of after,or
R2195 T2618 T2617 arg2Of stimulation,after
R2196 T2618 T2619 arg1Of stimulation,with
R2197 T2620 T2619 arg2Of LPS,with
R2198 T2622 T2621 arg1Of mRNA,Sharpin
R2199 T2622 T2623 arg1Of mRNA,was
R2200 T2622 T2624 arg1Of mRNA,present
R2201 T2623 T2635 arg1Of was,and
R2202 T2624 T2623 arg2Of present,was
R2203 T2624 T2625 arg1Of present,in
R2204 T2626 T2625 arg2Of BMDC,in
R2205 T2626 T2627 arg2Of BMDC,generated
R2206 T2627 T2628 arg1Of generated,from
R2207 T2627 T2631 arg1Of generated,(
R2208 T2630 T2628 arg2Of mice,from
R2209 T2630 T2629 arg1Of mice,WT
R2210 T2633 T2631 arg2Of 1D,(
R2211 T2633 T2632 arg1Of 1D,Fig.
R2212 T2634 T2631 arg3Of ),(
R2213 T2637 T2636 arg1Of level,its
R2214 T2637 T2638 arg1Of level,was
R2215 T2637 T2640 arg2Of level,decreased
R2216 T2640 T2635 arg2Of decreased,and
R2217 T2640 T2638 arg2Of decreased,was
R2218 T2640 T2639 arg1Of decreased,modestly
R2219 T2640 T2641 arg1Of decreased,by
R2220 T2643 T2641 arg2Of stimulation,by
R2221 T2643 T2642 arg1Of stimulation,LPS
R2222 T2644 T2645 arg1Of There,was
R2223 T2645 T2655 arg1Of was,in
R2224 T2648 T2645 arg2Of reduction,was
R2225 T2648 T2646 arg1Of reduction,a
R2226 T2648 T2647 arg1Of reduction,significant
R2227 T2648 T2649 arg1Of reduction,of
R2228 T2651 T2649 arg2Of mRNA,of
R2229 T2651 T2650 arg1Of mRNA,Sharpin
R2230 T2651 T2652 arg1Of mRNA,(
R2231 T2653 T2652 arg2Of 6–7-fold,(
R2232 T2654 T2652 arg3Of ),(
R2233 T2656 T2655 arg2Of BMDC,in
R2234 T2656 T2657 arg2Of BMDC,generated
R2235 T2657 T2658 arg1Of generated,from
R2236 T2660 T2658 arg2Of mice,from
R2237 T2660 T2659 arg1Of mice,cpdm
R2238 T2661 T2662 arg1Of Transfection,of
R2239 T2661 T2665 arg1Of Transfection,in
R2240 T2661 T2675 arg1Of Transfection,indicated
R2241 T2664 T2662 arg2Of Sharpin,of
R2242 T2664 T2663 arg1Of Sharpin,Flag-tagged
R2243 T2666 T2667 arg1Of fibroblasts,(
R2244 T2666 T2670 arg1Of fibroblasts,and
R2245 T2668 T2667 arg2Of NIH3T3,(
R2246 T2669 T2667 arg3Of ),(
R2247 T2670 T2665 arg2Of and,in
R2248 T2671 T2670 arg2Of macrophages,and
R2249 T2671 T2672 arg1Of macrophages,(
R2250 T2673 T2672 arg2Of RAW264.7,(
R2251 T2674 T2672 arg3Of ),(
R2252 T2677 T2675 arg2Of localization,indicated
R2253 T2677 T2676 arg1Of localization,cytoplasmic
R2254 T2677 T2678 arg1Of localization,of
R2255 T2681 T2678 arg2Of protein,of
R2256 T2681 T2679 arg1Of protein,the
R2257 T2681 T2680 arg1Of protein,SHARPIN
R2258 T2681 T2682 arg1Of protein,(
R2259 T2684 T2682 arg2Of 1E,(
R2260 T2684 T2683 arg1Of 1E,Fig.
R2261 T2685 T2682 arg3Of ),(
R2707 T3283 T3281 arg1Of DC,Phenotyping
R2708 T3283 T3282 arg1Of DC,splenic
R2709 T3283 T3284 arg1Of DC,and
R2710 T3285 T3284 arg2Of BMDC,and
R2711 T3285 T3286 arg1Of BMDC,from
R2712 T3287 T3288 arg1Of WT,and
R2715 T3290 T3286 arg2Of mice,from
R2716 T3290 T3287 arg1Of mice,WT
R2717 T3291 T3292 arg1Of DC,are
R2718 T3291 T3293 arg1Of DC,heterogeneous
R2719 T3291 T3295 arg1Of DC,can
R2720 T3291 T3296 arg1Of DC,be
R2721 T3291 T3297 arg2Of DC,categorized
R2722 T3292 T3294 arg1Of are,and
R2723 T3293 T3292 arg2Of heterogeneous,are
R2724 T3297 T3294 arg2Of categorized,and
R2725 T3297 T3295 arg2Of categorized,can
R2726 T3297 T3296 arg2Of categorized,be
R2727 T3297 T3298 arg1Of categorized,into
R2728 T3300 T3298 arg2Of subtypes,into
R2729 T3300 T3299 arg1Of subtypes,multiple
R2730 T3300 T3301 arg1Of subtypes,based
R2731 T3302 T3301 arg2Of on,based
R2732 T3304 T3302 arg2Of markers,on
R2733 T3304 T3303 arg1Of markers,surface
R2734 T3304 T3305 arg1Of markers,[
R2735 T3306 T3305 arg2Of 13,[
R2736 T3307 T3305 arg3Of ],[
R2737 T3309 T3308 arg1Of determine,To
R2738 T3313 T3311 arg1Of mutation,the
R2739 T3313 T3312 arg1Of mutation,Sharpin
R2740 T3313 T3314 arg1Of mutation,affects
R2741 T3314 T3309 arg2Of affects,determine
R2742 T3314 T3310 arg1Of affects,if
R2743 T3314 T3317 arg1Of affects,in
R2744 T3316 T3314 arg2Of development,affects
R2745 T3316 T3315 arg1Of development,DC
R2746 T3319 T3317 arg2Of tissues,in
R2747 T3319 T3318 arg1Of tissues,lymphoid
R2748 T3322 T3321 arg1Of spleens,mouse
R2749 T3322 T3323 arg1Of spleens,were
R2750 T3322 T3324 arg2Of spleens,examined
R2751 T3324 T3308 modOf examined,To
R2752 T3324 T3320 arg1Of examined,","
R2753 T3324 T3323 arg2Of examined,were
R2754 T3324 T3325 arg1Of examined,for
R2755 T3324 T3349 arg1Of examined,[
R2756 T3327 T3328 arg1Of distribution,of
R2757 T3327 T3331 arg1Of distribution,(
R2758 T3327 T3338 arg1Of distribution,[
R2759 T3327 T3341 arg1Of distribution,and
R2760 T3330 T3328 arg2Of DC,of
R2761 T3330 T3329 arg1Of DC,conventional
R2762 T3332 T3331 arg2Of cDC,(
R2763 T3332 T3333 arg1Of cDC,;
R2764 T3334 T3335 arg1Of CD11c+CD8α+,and
R2765 T3335 T3333 arg2Of and,;
R2766 T3336 T3335 arg2Of CD11c+CD8α−,and
R2767 T3337 T3331 arg3Of ),(
R2768 T3339 T3338 arg2Of 13,[
R2769 T3340 T3338 arg3Of ],[
R2770 T3341 T3325 arg2Of and,for
R2771 T3341 T3326 arg1Of and,the
R2772 T3343 T3341 arg2Of DC,and
R2773 T3343 T3342 arg1Of DC,plasmacytoid
R2774 T3343 T3344 arg1Of DC,(
R2775 T3345 T3344 arg2Of pDC,(
R2776 T3345 T3346 arg1Of pDC,;
R2777 T3345 T3347 arg1Of pDC,CD11c−PDCA-1+
R2778 T3348 T3344 arg3Of ),(
R2779 T3350 T3349 arg2Of 14,[
R2780 T3351 T3349 arg3Of ],[
R2781 T3353 T3352 arg1Of percentages,The
R2782 T3353 T3354 arg1Of percentages,for
R2783 T3353 T3359 arg1Of percentages,were
R2784 T3353 T3361 arg2Of percentages,reduced
R2785 T3353 T3366 arg1Of percentages,compared
R2786 T3356 T3357 arg1Of cDC,and
R2787 T3357 T3354 arg2Of and,for
R2788 T3357 T3355 arg1Of and,splenic
R2789 T3358 T3357 arg2Of pDC,and
R2790 T3361 T3359 arg2Of reduced,were
R2791 T3361 T3360 arg1Of reduced,both
R2792 T3361 T3362 arg1Of reduced,in
R2793 T3361 T3365 arg1Of reduced,when
R2794 T3364 T3362 arg2Of mice,in
R2795 T3364 T3363 arg1Of mice,cpdm
R2796 T3366 T3365 arg2Of compared,when
R2797 T3366 T3367 arg1Of compared,with
R2798 T3369 T3367 arg2Of controls,with
R2799 T3369 T3368 arg1Of controls,WT
R2800 T3369 T3370 arg1Of controls,(
R2801 T3372 T3370 arg2Of 2A,(
R2802 T3372 T3371 arg1Of 2A,Fig.
R2803 T3373 T3370 arg3Of ),(
R2804 T3377 T3376 arg2Of gated,when
R2805 T3377 T3378 arg1Of gated,on
R2806 T3380 T3378 arg2Of cDC,on
R2807 T3380 T3379 arg1Of cDC,CD11c+
R2808 T3383 T3377 arg1Of percentages,gated
R2809 T3383 T3382 arg1Of percentages,the
R2810 T3383 T3384 arg1Of percentages,of
R2811 T3383 T3389 arg1Of percentages,were
R2812 T3383 T3391 arg2Of percentages,affected
R2813 T3388 T3384 arg2Of cells,of
R2814 T3388 T3385 arg1Of cells,CD8α+
R2815 T3388 T3386 arg1Of cells,and
R2816 T3388 T3387 arg1Of cells,CD8α−
R2817 T3391 T3374 arg1Of affected,However
R2818 T3391 T3375 arg1Of affected,","
R2819 T3391 T3376 arg1Of affected,when
R2820 T3391 T3381 arg1Of affected,","
R2821 T3391 T3389 arg2Of affected,were
R2822 T3391 T3390 arg1Of affected,not
R2823 T3394 T3391 arg1Of deficiency,affected
R2824 T3394 T3392 arg2Of deficiency,by
R2825 T3394 T3393 arg1Of deficiency,SHARPIN
R2826 T3394 T3395 arg1Of deficiency,(
R2827 T3397 T3395 arg2Of 2A,(
R2828 T3397 T3396 arg1Of 2A,Fig.
R2829 T3398 T3395 arg3Of ),(
R2830 T3401 T3400 arg1Of spleen,the
R2831 T3401 T3402 arg1Of spleen,of
R2832 T3401 T3405 arg1Of spleen,is
R2833 T3401 T3407 arg2Of spleen,enlarged
R2834 T3401 T3409 arg1Of spleen,contains
R2835 T3404 T3402 arg2Of mice,of
R2836 T3404 T3403 arg1Of mice,cpdm
R2837 T3407 T3405 arg2Of enlarged,is
R2838 T3407 T3406 arg1Of enlarged,markedly
R2839 T3407 T3408 arg1Of enlarged,and
R2840 T3408 T3399 arg2Of and,Since
R2841 T3409 T3408 arg2Of contains,and
R2842 T3409 T3412 arg1Of contains,as
R2843 T3411 T3409 arg2Of times,contains
R2844 T3411 T3410 arg1Of times,three
R2845 T3414 T3412 arg2Of cells,as
R2846 T3414 T3413 arg1Of cells,many
R2847 T3414 T3415 arg1Of cells,(
R2848 T3417 T3415 arg2Of 1C,(
R2849 T3417 T3416 arg1Of 1C,Fig.
R2850 T3418 T3415 arg3Of ),(
R2851 T3422 T3420 arg1Of percentages,the
R2852 T3422 T3421 arg1Of percentages,different
R2853 T3422 T3423 arg1Of percentages,of
R2854 T3422 T3433 arg1Of percentages,reflect
R2855 T3425 T3426 arg1Of cDC,and
R2856 T3426 T3423 arg2Of and,of
R2857 T3426 T3424 arg1Of and,splenic
R2858 T3426 T3428 arg1Of and,between
R2859 T3427 T3426 arg2Of pDC,and
R2860 T3429 T3430 arg1Of WT,and
R2861 T3431 T3430 arg2Of mutant,and
R2862 T3432 T3428 arg2Of mice,between
R2863 T3432 T3429 arg1Of mice,WT
R2864 T3432 T3431 arg1Of mice,mutant
R2865 T3433 T3399 arg1Of reflect,Since
R2866 T3433 T3419 arg1Of reflect,","
R2867 T3436 T3434 arg1Of number,the
R2868 T3436 T3435 arg2Of number,increased
R2869 T3436 T3437 arg1Of number,of
R2870 T3436 T3442 arg1Of number,than
R2871 T3440 T3437 arg2Of cells,of
R2872 T3440 T3438 arg1Of cells,total
R2873 T3440 T3439 arg1Of cells,spleen
R2874 T3442 T3433 arg2Of than,reflect
R2875 T3442 T3441 arg1Of than,rather
R2876 T3444 T3442 arg2Of reduction,than
R2877 T3444 T3443 arg1Of reduction,a
R2878 T3444 T3445 arg1Of reduction,in
R2879 T3446 T3447 arg1Of cDC,and
R2880 T3448 T3447 arg2Of pDC,and
R2881 T3449 T3445 arg2Of numbers,in
R2882 T3449 T3446 arg1Of numbers,cDC
R2883 T3449 T3448 arg1Of numbers,pDC
R2884 T3451 T3450 arg1Of data,These
R2885 T3451 T3452 arg1Of data,indicate
R2886 T3456 T3454 arg1Of mutation,the
R2887 T3456 T3455 arg1Of mutation,Sharpin
R2888 T3456 T3457 arg1Of mutation,does
R2889 T3456 T3459 arg1Of mutation,affect
R2890 T3459 T3452 arg2Of affect,indicate
R2891 T3459 T3453 arg1Of affect,that
R2892 T3459 T3457 arg2Of affect,does
R2893 T3459 T3458 arg1Of affect,not
R2894 T3461 T3459 arg2Of distribution,affect
R2895 T3461 T3460 arg1Of distribution,the
R2896 T3461 T3462 arg1Of distribution,of
R2897 T3466 T3462 arg2Of subsets,of
R2898 T3466 T3463 arg1Of subsets,the
R2899 T3466 T3464 arg2Of subsets,examined
R2900 T3466 T3465 arg1Of subsets,DC
R2901 T3466 T3467 arg1Of subsets,in
R2902 T3469 T3467 arg2Of spleen,in
R2903 T3469 T3468 arg1Of spleen,the
R2904 T3470 T3471 arg1Of BMDC,from
R2905 T3470 T3476 arg1Of BMDC,resemble
R2906 T3473 T3472 arg1Of vitro,in
R2907 T3474 T3471 arg2Of cultures,from
R2908 T3474 T3473 arg1Of cultures,vitro
R2909 T3476 T3475 arg1Of resemble,functionally
R2910 T3476 T3487 arg1Of resemble,","
R2911 T3476 T3488 arg1Of resemble,[
R2912 T3479 T3477 arg1Of DC,non-lymphoid
R2913 T3479 T3478 arg1Of DC,tissue
R2914 T3479 T3480 arg1Of DC,and
R2915 T3480 T3476 arg2Of and,resemble
R2916 T3483 T3480 arg2Of DC,and
R2917 T3483 T3481 arg1Of DC,monocyte-derived
R2918 T3483 T3482 arg1Of DC,inflammatory
R2919 T3483 T3484 arg1Of DC,[
R2920 T3485 T3484 arg2Of 15,[
R2921 T3486 T3484 arg3Of ],[
R2922 T3489 T3488 arg2Of 16,[
R2923 T3490 T3488 arg3Of ],[
R2924 T3492 T3491 arg1Of yields,The
R2925 T3492 T3493 arg1Of yields,of
R2926 T3492 T3500 arg1Of yields,were
R2927 T3492 T3501 arg1Of yields,similar
R2928 T3494 T3493 arg2Of BMDC,of
R2929 T3494 T3495 arg1Of BMDC,from
R2930 T3496 T3497 arg1Of WT,and
R2931 T3498 T3497 arg2Of cpdm,and
R2932 T3499 T3495 arg2Of mice,from
R2933 T3499 T3496 arg1Of mice,WT
R2934 T3499 T3498 arg1Of mice,cpdm
R2935 T3501 T3500 arg2Of similar,were
R2936 T3502 T3503 arg1Of BMDC,were
R2937 T3502 T3504 arg1Of BMDC,CD11c+
R2938 T3502 T3507 arg1Of BMDC,II+
R2939 T3504 T3505 arg1Of CD11c+,and
R2940 T3505 T3503 arg2Of and,were
R2941 T3505 T3508 arg1Of and,with
R2942 T3507 T3505 arg2Of II+,and
R2943 T3507 T3506 arg1Of II+,MHC
R2944 T3510 T3508 arg2Of expression,with
R2945 T3510 T3509 arg1Of expression,low
R2946 T3510 T3511 arg1Of expression,of
R2947 T3514 T3515 arg1Of CD40,","
R2948 T3515 T3518 arg1Of ",",and
R2949 T3516 T3515 arg2Of CD80,","
R2950 T3518 T3511 arg2Of and,of
R2951 T3518 T3512 arg1Of and,co-stimulatory
R2952 T3518 T3513 arg1Of and,molecules
R2953 T3518 T3517 arg1Of and,","
R2954 T3519 T3518 arg2Of CD86,and
R2955 T3524 T3521 arg1Of I,TLR3
R2956 T3524 T3522 arg1Of I,ligand
R2957 T3524 T3523 arg1Of I,poly
R2958 T3524 T3525 arg1Of I,:
R2959 T3526 T3520 arg1Of C,The
R2960 T3526 T3524 arg1Of C,I
R2961 T3526 T3527 arg1Of C,and
R2962 T3527 T3533 arg1Of and,activate
R2963 T3527 T3540 arg1Of and,were
R2964 T3527 T3541 arg2Of and,used
R2965 T3531 T3527 arg2Of LPS,and
R2966 T3531 T3528 arg1Of LPS,the
R2967 T3531 T3529 arg1Of LPS,TLR4
R2968 T3531 T3530 arg1Of LPS,ligand
R2969 T3533 T3532 arg1Of activate,each
R2970 T3533 T3539 arg1Of activate,and
R2971 T3534 T3535 arg1Of overlapping,but
R2972 T3536 T3535 arg2Of different,but
R2973 T3538 T3533 arg2Of pathways,activate
R2974 T3538 T3534 arg1Of pathways,overlapping
R2975 T3538 T3536 arg1Of pathways,different
R2976 T3538 T3537 arg1Of pathways,signaling
R2977 T3539 T3549 arg1Of and,","
R2978 T3539 T3550 arg1Of and,[
R2979 T3541 T3539 arg2Of used,and
R2980 T3541 T3540 arg2Of used,were
R2981 T3543 T3541 arg3Of induce,used
R2982 T3543 T3542 arg1Of induce,to
R2983 T3545 T3543 arg2Of maturation,induce
R2984 T3545 T3544 arg1Of maturation,DC
R2985 T3545 T3546 arg1Of maturation,[
R2986 T3547 T3546 arg2Of 17,[
R2987 T3548 T3546 arg3Of ],[
R2988 T3551 T3550 arg2Of 18,[
R2989 T3552 T3550 arg3Of ],[
R2990 T3553 T3554 arg1Of Incubation,with
R2991 T3553 T3561 arg1Of Incubation,resulted
R2992 T3557 T3554 arg2Of agonists,with
R2993 T3557 T3555 arg1Of agonists,the
R2994 T3557 T3556 arg1Of agonists,TLR
R2995 T3557 T3558 arg1Of agonists,for
R2996 T3560 T3558 arg2Of hours,for
R2997 T3560 T3559 arg1Of hours,24
R2998 T3561 T3562 arg1Of resulted,in
R2999 T3561 T3574 arg1Of resulted,;
R3000 T3564 T3562 arg2Of expression,in
R3001 T3564 T3563 arg2Of expression,increased
R3002 T3564 T3565 arg1Of expression,of
R3003 T3564 T3572 arg1Of expression,on
R3004 T3566 T3567 arg1Of CD40,","
R3005 T3567 T3570 arg1Of ",",and
R3006 T3568 T3567 arg2Of CD80,","
R3007 T3570 T3565 arg2Of and,of
R3008 T3570 T3569 arg1Of and,","
R3009 T3571 T3570 arg2Of CD86,and
R3010 T3573 T3572 arg2Of BMDC,on
R3011 T3577 T3578 arg1Of there,was
R3012 T3578 T3574 arg2Of was,;
R3013 T3578 T3575 arg1Of was,however
R3014 T3578 T3576 arg1Of was,","
R3015 T3580 T3578 arg2Of difference,was
R3016 T3580 T3579 arg1Of difference,no
R3017 T3580 T3581 arg1Of difference,in
R3018 T3580 T3588 arg1Of difference,between
R3019 T3584 T3581 arg2Of levels,in
R3020 T3584 T3582 arg1Of levels,the
R3021 T3584 T3583 arg1Of levels,expression
R3022 T3584 T3585 arg1Of levels,of
R3023 T3587 T3585 arg2Of markers,of
R3024 T3587 T3586 arg1Of markers,these
R3025 T3589 T3590 arg1Of WT,and
R3026 T3590 T3588 arg2Of and,between
R3027 T3592 T3590 arg2Of BMDC,and
R3028 T3592 T3591 arg1Of BMDC,cpdm
R3029 T3592 T3593 arg1Of BMDC,(
R3030 T3595 T3593 arg2Of 2B,(
R3031 T3595 T3594 arg1Of 2B,Fig.
R3032 T3596 T3593 arg3Of ),(
R3033 T3600 T3599 arg1Of deficiency,SHARPIN
R3034 T3600 T3601 arg1Of deficiency,did
R3035 T3600 T3603 arg1Of deficiency,influence
R3036 T3603 T3597 arg1Of influence,Thus
R3037 T3603 T3598 arg1Of influence,","
R3038 T3603 T3601 arg2Of influence,did
R3039 T3603 T3602 arg1Of influence,not
R3040 T3605 T3603 arg2Of expression,influence
R3041 T3605 T3604 arg1Of expression,the
R3042 T3605 T3606 arg1Of expression,of
R3043 T3605 T3609 arg1Of expression,by
R3044 T3608 T3606 arg2Of molecules,of
R3045 T3608 T3607 arg1Of molecules,co-stimulatory
R3046 T3610 T3609 arg2Of BMDC,by
R3323 T4014 T4015 arg1Of Production,of
R3324 T4014 T4018 arg1Of Production,by
R3325 T4017 T4015 arg2Of mediators,of
R3326 T4017 T4016 arg1Of mediators,proinflammatory
R3328 T4020 T4018 arg2Of BMDC,by
R3331 T4023 T4031 arg1Of Incubation,resulted
R3332 T4025 T4026 arg1Of LPS,or
R3333 T4026 T4024 arg2Of or,with
R3334 T4030 T4026 arg2Of C,or
R3335 T4030 T4027 arg1Of C,poly
R3336 T4030 T4028 arg1Of C,I
R3337 T4030 T4029 arg1Of C,:
R3338 T4031 T4032 arg1Of resulted,in
R3339 T4031 T4047 arg1Of resulted,;
R3340 T4033 T4032 arg2Of secretion,in
R3341 T4033 T4034 arg1Of secretion,of
R3342 T4033 T4041 arg1Of secretion,from
R3343 T4035 T4036 arg1Of IL6,","
R3344 T4036 T4039 arg1Of ",",and
R3345 T4037 T4036 arg2Of IL12P70,","
R3346 T4039 T4034 arg2Of and,of
R3347 T4039 T4038 arg1Of and,","
R3348 T4040 T4039 arg2Of GMCSF,and
R3349 T4043 T4044 arg1Of WT,and
R3350 T4044 T4042 arg1Of and,both
R3351 T4045 T4044 arg2Of cpdm,and
R3352 T4046 T4041 arg2Of BMDC,from
R3353 T4046 T4043 arg1Of BMDC,WT
R3354 T4046 T4045 arg1Of BMDC,cpdm
R3355 T4050 T4051 arg1Of BMDC,from
R3356 T4050 T4054 arg1Of BMDC,produced
R3357 T4053 T4051 arg2Of mice,from
R3358 T4053 T4052 arg1Of mice,cpdm
R3359 T4054 T4047 arg2Of produced,;
R3360 T4054 T4048 arg1Of produced,however
R3361 T4054 T4049 arg1Of produced,","
R3362 T4054 T4055 arg1Of produced,significantly
R3363 T4054 T4061 arg1Of produced,compared
R3364 T4056 T4054 arg2Of less,produced
R3365 T4056 T4057 arg1Of less,of
R3366 T4060 T4057 arg2Of cytokines,of
R3367 T4060 T4058 arg1Of cytokines,all
R3368 T4060 T4059 arg1Of cytokines,three
R3369 T4062 T4061 arg2Of with,compared
R3370 T4064 T4062 arg2Of BMDC,with
R3371 T4064 T4063 arg1Of BMDC,WT
R3372 T4064 T4065 arg1Of BMDC,(
R3373 T4067 T4065 arg2Of 3A–C,(
R3374 T4067 T4066 arg1Of 3A–C,Fig.
R3375 T4067 T4068 arg1Of 3A–C,","
R3376 T4069 T4068 arg2Of E–F,","
R3377 T4070 T4065 arg3Of ),(
R3378 T4072 T4071 arg1Of amount,The
R3379 T4072 T4073 arg1Of amount,of
R3380 T4072 T4080 arg1Of amount,was
R3381 T4075 T4073 arg2Of oxide,of
R3382 T4075 T4074 arg1Of oxide,nitric
R3383 T4075 T4076 arg2Of oxide,generated
R3384 T4076 T4077 arg1Of generated,by
R3385 T4079 T4077 arg2Of BMDC,by
R3386 T4079 T4078 arg1Of BMDC,mutant
R3387 T4080 T4081 arg1Of was,also
R3388 T4080 T4091 arg1Of was,)
R3389 T4080 T4092 arg1Of was,","
R3390 T4080 T4093 modOf was,indicating
R3391 T4083 T4082 arg1Of reduced,significantly
R3392 T4083 T4084 arg1Of reduced,compared
R3393 T4085 T4084 arg2Of with,compared
R3394 T4087 T4085 arg2Of cells,with
R3395 T4087 T4086 arg1Of cells,WT
R3396 T4090 T4080 arg2Of 3D,was
R3397 T4090 T4083 arg2Of 3D,reduced
R3398 T4090 T4088 arg1Of 3D,(
R3399 T4090 T4089 arg1Of 3D,Fig.
R3400 T4096 T4093 arg2Of production,indicating
R3401 T4096 T4094 arg1Of production,severely
R3402 T4096 T4095 arg2Of production,disrupted
R3403 T4096 T4097 arg1Of production,of
R3404 T4099 T4097 arg2Of mediators,of
R3405 T4099 T4098 arg1Of mediators,proinflammatory
R3406 T4099 T4100 arg1Of mediators,from
R3407 T4102 T4100 arg2Of BMDC,from
R3408 T4102 T4101 arg1Of BMDC,Sharpin-deficient
R3409 T4104 T4103 arg1Of vivo,In
R3410 T4105 T4104 arg1Of complementation,vivo
R3411 T4105 T4106 arg1Of complementation,with
R3412 T4105 T4111 arg1Of complementation,reversed
R3413 T4110 T4106 arg2Of BAC,with
R3414 T4110 T4107 arg1Of BAC,a
R3415 T4110 T4108 arg1Of BAC,Sharpin
R3416 T4110 T4109 arg1Of BAC,gene-containing
R3417 T4111 T4122 arg1Of reversed,and
R3418 T4113 T4111 arg2Of phenotype,reversed
R3419 T4113 T4112 arg1Of phenotype,the
R3420 T4113 T4114 arg1Of phenotype,of
R3421 T4117 T4114 arg2Of mice,of
R3422 T4117 T4115 arg1Of mice,the
R3423 T4117 T4116 arg1Of mice,mutant
R3424 T4117 T4118 arg1Of mice,[
R3425 T4119 T4118 arg2Of 2,[
R3426 T4120 T4118 arg3Of ],[
R3427 T4122 T4121 arg1Of and,","
R3428 T4123 T4124 arg2Of BMDC,generated
R3429 T4123 T4129 arg1Of BMDC,secreted
R3430 T4124 T4125 arg1Of generated,from
R3431 T4128 T4125 arg2Of mice,from
R3432 T4128 T4126 arg1Of mice,these
R3433 T4128 T4127 arg2Of mice,rescued
R3434 T4129 T4122 arg2Of secreted,and
R3435 T4129 T4142 arg1Of secreted,","
R3436 T4129 T4143 modOf secreted,supporting
R3437 T4131 T4130 arg1Of more,significantly
R3438 T4132 T4129 arg2Of IL12P70,secreted
R3439 T4132 T4131 arg1Of IL12P70,more
R3440 T4132 T4133 arg1Of IL12P70,than
R3441 T4134 T4133 arg2Of BMDC,than
R3442 T4134 T4135 arg1Of BMDC,from
R3443 T4137 T4135 arg2Of mice,from
R3444 T4137 T4136 arg1Of mice,cpdm
R3445 T4137 T4138 arg1Of mice,(
R3446 T4140 T4138 arg2Of 3G,(
R3447 T4140 T4139 arg1Of 3G,Fig.
R3448 T4141 T4138 arg3Of ),(
R3449 T4146 T4143 arg2Of role,supporting
R3450 T4146 T4144 arg1Of role,a
R3451 T4146 T4145 arg1Of role,necessary
R3452 T4146 T4147 arg1Of role,of
R3453 T4146 T4149 arg1Of role,for
R3454 T4148 T4147 arg2Of SHARPIN,of
R3455 T4151 T4149 arg2Of production,for
R3456 T4151 T4150 arg1Of production,the
R3457 T4151 T4152 arg1Of production,of
R3458 T4153 T4152 arg2Of IL12P70,of
R3459 T4155 T4154 arg2Of addition,In
R3460 T4159 T4157 arg1Of levels,the
R3461 T4159 T4158 arg1Of levels,transcript
R3462 T4159 T4160 arg1Of levels,of
R3463 T4159 T4172 arg1Of levels,were
R3464 T4159 T4173 arg2Of levels,examined
R3465 T4163 T4161 arg1Of cytokines,the
R3466 T4163 T4162 arg1Of cytokines,inflammatory
R3467 T4164 T4165 arg1Of Il6,","
R3468 T4165 T4167 arg1Of ",",","
R3469 T4166 T4165 arg2Of Il12p40,","
R3470 T4167 T4170 arg1Of ",",and
R3471 T4168 T4167 arg2Of Gmcsf,","
R3472 T4170 T4160 arg2Of and,of
R3473 T4170 T4163 arg1Of and,cytokines
R3474 T4170 T4169 arg1Of and,","
R3475 T4171 T4170 arg2Of Ifnb,and
R3476 T4173 T4172 arg2Of examined,were
R3477 T4173 T4175 arg1Of examined,and
R3478 T4175 T4154 arg1Of and,In
R3479 T4175 T4156 arg1Of and,","
R3480 T4175 T4174 arg1Of and,","
R3481 T4176 T4177 arg1Of these,were
R3482 T4176 T4180 arg2Of these,reduced
R3483 T4176 T4186 arg1Of these,compared
R3484 T4180 T4175 arg2Of reduced,and
R3485 T4180 T4177 arg2Of reduced,were
R3486 T4180 T4178 arg1Of reduced,all
R3487 T4180 T4179 arg1Of reduced,significantly
R3488 T4180 T4181 arg1Of reduced,in
R3489 T4180 T4185 arg1Of reduced,when
R3490 T4184 T4181 arg2Of BMDC,in
R3491 T4184 T4182 arg2Of BMDC,stimulated
R3492 T4184 T4183 arg1Of BMDC,cpdm
R3493 T4186 T4185 arg2Of compared,when
R3494 T4186 T4187 arg1Of compared,with
R3495 T4189 T4187 arg2Of controls,with
R3496 T4189 T4188 arg1Of controls,WT
R3497 T4189 T4190 arg1Of controls,(
R3498 T4191 T4190 arg2Of Fig.,(
R3499 T4191 T4192 arg1Of Fig.,4
R3500 T4193 T4190 arg3Of ),(
R4047 T4914 T4912 arg1Of production,Impaired
R4048 T4914 T4913 arg1Of production,cytokine
R4049 T4914 T4915 arg1Of production,by
R4050 T4914 T4918 arg1Of production,is
R4051 T4914 T4919 arg2Of production,correlated
R4052 T4917 T4915 arg2Of BMDC,by
R4053 T4917 T4916 arg1Of BMDC,cpdm
R4054 T4919 T4918 arg2Of correlated,is
R4055 T4919 T4920 arg1Of correlated,with
R4056 T4922 T4920 arg2Of defects,with
R4057 T4922 T4921 arg1Of defects,selective
R4058 T4922 T4923 arg1Of defects,in
R4059 T4925 T4923 arg2Of signaling,in
R4060 T4925 T4924 arg1Of signaling,NF-κB
R4061 T4926 T4927 arg1Of There,are
R4062 T4929 T4927 arg2Of number,are
R4063 T4929 T4928 arg1Of number,a
R4064 T4929 T4930 arg1Of number,of
R4065 T4929 T4933 arg1Of number,for
R4066 T4932 T4930 arg2Of explanations,of
R4067 T4932 T4931 arg1Of explanations,possible
R4068 T4937 T4935 arg1Of secretion,defective
R4069 T4937 T4936 arg1Of secretion,cytokine
R4070 T4937 T4938 arg1Of secretion,in
R4071 T4937 T4942 arg1Of secretion,","
R4072 T4937 T4943 arg1Of secretion,including
R4073 T4937 T4956 arg1Of secretion,)
R4074 T4937 T4965 modOf secretion,increased
R4075 T4937 T4974 arg1Of secretion,and
R4076 T4941 T4938 arg2Of BMDC,in
R4077 T4941 T4939 arg2Of BMDC,stimulated
R4078 T4941 T4940 arg1Of BMDC,cpdm
R4079 T4948 T4943 arg2Of expression,including
R4080 T4948 T4944 arg1Of expression,1
R4081 T4948 T4945 arg1Of expression,)
R4082 T4948 T4946 arg2Of expression,reduced
R4083 T4948 T4947 arg1Of expression,surface
R4084 T4948 T4949 arg1Of expression,of
R4085 T4953 T4949 arg2Of complex,of
R4086 T4953 T4950 arg1Of complex,the
R4087 T4953 T4951 arg1Of complex,LPS
R4088 T4953 T4952 arg1Of complex,receptor
R4089 T4953 T4954 arg1Of complex,","
R4090 T4955 T4954 arg2Of 2,","
R4091 T4958 T4957 arg2Of production,increased
R4092 T4958 T4959 arg1Of production,of
R4093 T4958 T4965 arg1Of production,increased
R4094 T4961 T4959 arg2Of mediators,of
R4095 T4961 T4960 arg1Of mediators,anti-inflammatory
R4096 T4961 T4962 arg1Of mediators,","
R4097 T4961 T4964 arg1Of mediators,)
R4098 T4963 T4962 arg2Of 3,","
R4099 T4966 T4965 arg2Of expression,increased
R4100 T4966 T4967 arg1Of expression,of
R4101 T4969 T4967 arg2Of regulators,of
R4102 T4969 T4968 arg1Of regulators,negative
R4103 T4969 T4970 arg1Of regulators,of
R4104 T4972 T4970 arg2Of pathways,of
R4105 T4972 T4971 arg1Of pathways,TLR
R4106 T4974 T4933 arg2Of and,for
R4107 T4974 T4934 arg1Of and,the
R4108 T4974 T4973 arg1Of and,","
R4109 T4980 T4974 arg2Of activation,and
R4110 T4980 T4975 arg1Of activation,4
R4111 T4980 T4976 arg1Of activation,)
R4112 T4980 T4977 arg1Of activation,impaired
R4113 T4980 T4978 arg1Of activation,TLR-induced
R4114 T4980 T4979 arg1Of activation,signaling
R4115 T4981 T4982 arg1Of We,determined
R4116 T4985 T4982 arg2Of expression,determined
R4117 T4985 T4983 arg1Of expression,the
R4118 T4985 T4984 arg1Of expression,surface
R4119 T4985 T4986 arg1Of expression,of
R4120 T4985 T4991 arg1Of expression,that
R4121 T4985 T4992 arg1Of expression,comprises
R4122 T4990 T4986 arg2Of complex,of
R4123 T4990 T4987 arg1Of complex,the
R4124 T4990 T4988 arg1Of complex,LPS
R4125 T4990 T4989 arg1Of complex,receptor
R4126 T4992 T5007 arg1Of comprises,[
R4127 T4993 T4994 arg1Of TLR4,","
R4128 T4994 T4999 arg1Of ",",and
R4129 T4997 T4995 arg1Of proteins,the
R4130 T4997 T4996 arg1Of proteins,accessory
R4131 T4998 T4994 arg2Of CD14,","
R4132 T4998 T4997 arg1Of CD14,proteins
R4133 T4999 T4992 arg2Of and,comprises
R4134 T5002 T4999 arg2Of factor,and
R4135 T5002 T5000 arg1Of factor,myeloid
R4136 T5002 T5001 arg1Of factor,differentiation
R4137 T5002 T5003 arg1Of factor,2
R4138 T5002 T5004 arg1Of factor,(
R4139 T5005 T5004 arg2Of MD2/LY96,(
R4140 T5006 T5004 arg3Of ),(
R4141 T5008 T5007 arg2Of 19,[
R4142 T5009 T5007 arg3Of ],[
R4143 T5012 T5010 arg1Of analysis,Flow
R4144 T5012 T5011 arg1Of analysis,cytometric
R4145 T5012 T5013 arg1Of analysis,shows
R4146 T5017 T5015 arg1Of levels,the
R4147 T5017 T5016 arg1Of levels,expression
R4148 T5017 T5018 arg1Of levels,of
R4149 T5017 T5022 arg1Of levels,between
R4150 T5017 T5027 arg1Of levels,were
R4151 T5017 T5028 arg1Of levels,similar
R4152 T5019 T5020 arg1Of CD14,and
R4153 T5020 T5018 arg2Of and,of
R4154 T5021 T5020 arg2Of TLR4/MD2,and
R4155 T5023 T5024 arg1Of WT,and
R4156 T5024 T5022 arg2Of and,between
R4157 T5026 T5024 arg2Of BMDC,and
R4158 T5026 T5025 arg1Of BMDC,cpdm
R4159 T5027 T5013 arg2Of were,shows
R4160 T5027 T5014 arg1Of were,that
R4161 T5028 T5027 arg2Of similar,were
R4162 T5028 T5029 arg1Of similar,(
R4163 T5031 T5029 arg2Of 5A,(
R4164 T5031 T5030 arg1Of 5A,Fig.
R4165 T5032 T5029 arg3Of ),(
R4166 T5033 T5035 arg1Of We,quantified
R4167 T5035 T5034 arg1Of quantified,then
R4168 T5037 T5035 arg2Of secretion,quantified
R4169 T5037 T5036 arg1Of secretion,the
R4170 T5037 T5038 arg1Of secretion,of
R4171 T5041 T5039 arg1Of cytokines,the
R4172 T5041 T5040 arg1Of cytokines,suppressive
R4173 T5042 T5038 arg2Of IL10,of
R4174 T5042 T5041 arg1Of IL10,cytokines
R4175 T5042 T5043 arg1Of IL10,that
R4176 T5042 T5044 arg1Of IL10,can
R4177 T5042 T5045 arg1Of IL10,inhibit
R4178 T5045 T5044 arg2Of inhibit,can
R4179 T5045 T5048 arg1Of inhibit,in
R4180 T5045 T5055 arg1Of inhibit,","
R4181 T5045 T5056 arg1Of inhibit,[
R4182 T5047 T5045 arg2Of secretion,inhibit
R4183 T5047 T5046 arg1Of secretion,IL12
R4184 T5051 T5048 arg2Of manner,in
R4185 T5051 T5049 arg1Of manner,an
R4186 T5051 T5050 arg1Of manner,autocrine
R4187 T5051 T5052 arg1Of manner,[
R4188 T5053 T5052 arg2Of 20,[
R4189 T5054 T5052 arg3Of ],[
R4190 T5057 T5056 arg2Of 21,[
R4191 T5058 T5056 arg3Of ],[
R4192 T5060 T5059 arg1Of supernatants,The
R4193 T5060 T5061 arg1Of supernatants,from
R4194 T5060 T5065 arg1Of supernatants,contained
R4195 T5064 T5061 arg2Of BMDC,from
R4196 T5064 T5062 arg1Of BMDC,LPS-stimulated
R4197 T5064 T5063 arg1Of BMDC,cpdm
R4198 T5065 T5079 arg1Of contained,","
R4199 T5065 T5080 modOf contained,suggesting
R4200 T5067 T5066 arg1Of lower,significantly
R4201 T5068 T5065 arg2Of levels,contained
R4202 T5068 T5067 arg1Of levels,lower
R4203 T5068 T5069 arg1Of levels,of
R4204 T5068 T5071 arg1Of levels,than
R4205 T5070 T5069 arg2Of IL10,of
R4206 T5074 T5071 arg2Of BMDC,than
R4207 T5074 T5072 arg2Of BMDC,stimulated
R4208 T5074 T5073 arg1Of BMDC,WT
R4209 T5074 T5075 arg1Of BMDC,(
R4210 T5077 T5075 arg2Of 5B,(
R4211 T5077 T5076 arg1Of 5B,Fig.
R4212 T5078 T5075 arg3Of ),(
R4213 T5082 T5083 arg1Of IL10,was
R4214 T5082 T5085 arg1Of IL10,responsible
R4215 T5083 T5080 arg2Of was,suggesting
R4216 T5083 T5081 arg1Of was,that
R4217 T5083 T5084 arg1Of was,not
R4218 T5085 T5083 arg2Of responsible,was
R4219 T5085 T5086 arg1Of responsible,for
R4220 T5088 T5086 arg2Of secretion,for
R4221 T5088 T5087 arg2Of secretion,decreased
R4222 T5088 T5089 arg1Of secretion,of
R4223 T5090 T5089 arg2Of IL12P70,of
R4224 T5090 T5091 arg1Of IL12P70,by
R4225 T5093 T5091 arg2Of BMDC,by
R4226 T5093 T5092 arg1Of BMDC,cpdm
R4227 T5095 T5094 arg2Of expression,Increased
R4228 T5095 T5096 arg1Of expression,of
R4229 T5095 T5109 arg1Of expression,may
R4230 T5095 T5111 arg1Of expression,suppress
R4231 T5099 T5096 arg2Of regulator,of
R4232 T5099 T5097 arg1Of regulator,a
R4233 T5099 T5098 arg1Of regulator,negative
R4234 T5099 T5100 arg1Of regulator,of
R4235 T5099 T5104 arg1Of regulator,as
R4236 T5102 T5100 arg2Of signaling,of
R4237 T5102 T5101 arg1Of signaling,TLR
R4238 T5104 T5103 arg1Of as,such
R4239 T5105 T5104 arg2Of A20,as
R4240 T5105 T5106 arg1Of A20,[
R4241 T5107 T5106 arg2Of 22,[
R4242 T5108 T5106 arg3Of ],[
R4243 T5111 T5109 arg2Of suppress,may
R4244 T5111 T5110 arg1Of suppress,also
R4245 T5113 T5111 arg2Of secretion,suppress
R4246 T5113 T5112 arg1Of secretion,cytokine
R4247 T5118 T5116 arg1Of level,the
R4248 T5118 T5117 arg1Of level,transcript
R4249 T5118 T5119 arg1Of level,of
R4250 T5118 T5121 arg1Of level,was
R4251 T5118 T5122 arg1Of level,lower
R4252 T5118 T5136 arg1Of level,ruling
R4253 T5120 T5119 arg2Of A20,of
R4254 T5121 T5114 arg1Of was,However
R4255 T5121 T5115 arg1Of was,","
R4256 T5121 T5130 arg1Of was,(
R4257 T5121 T5134 arg1Of was,","
R4258 T5121 T5136 modOf was,ruling
R4259 T5122 T5121 arg2Of lower,was
R4260 T5122 T5123 arg1Of lower,in
R4261 T5122 T5127 arg1Of lower,than
R4262 T5126 T5123 arg2Of BMDC,in
R4263 T5126 T5124 arg1Of BMDC,LPS-activated
R4264 T5126 T5125 arg1Of BMDC,cpdm
R4265 T5129 T5127 arg2Of controls,than
R4266 T5129 T5128 arg1Of controls,WT
R4267 T5132 T5130 arg2Of 5C,(
R4268 T5132 T5131 arg1Of 5C,Fig.
R4269 T5133 T5130 arg3Of ),(
R4270 T5136 T5135 arg1Of ruling,thereby
R4271 T5136 T5137 arg1Of ruling,out
R4272 T5136 T5141 arg1Of ruling,as
R4273 T5138 T5136 arg2Of overexpression,ruling
R4274 T5138 T5139 arg1Of overexpression,of
R4275 T5140 T5139 arg2Of A20,of
R4276 T5143 T5141 arg2Of factor,as
R4277 T5143 T5142 arg1Of factor,a
R4278 T5143 T5144 arg1Of factor,in
R4279 T5148 T5144 arg2Of production,in
R4280 T5148 T5145 arg1Of production,the
R4281 T5148 T5146 arg2Of production,reduced
R4282 T5148 T5147 arg1Of production,cytokine
R4283 T5150 T5149 arg1Of transcription,The
R4284 T5150 T5151 arg1Of transcription,of
R4285 T5150 T5155 arg1Of transcription,is
R4286 T5150 T5157 arg2Of transcription,regulated
R4287 T5154 T5151 arg2Of intermediates,of
R4288 T5154 T5152 arg1Of intermediates,TLR3/4-induced
R4289 T5154 T5153 arg1Of intermediates,proinflammatory
R4290 T5157 T5155 arg2Of regulated,is
R4291 T5157 T5156 arg1Of regulated,tightly
R4292 T5157 T5162 arg1Of regulated,","
R4293 T5157 T5163 arg1Of regulated,in
R4294 T5161 T5157 arg1Of pathways,regulated
R4295 T5161 T5158 arg2Of pathways,by
R4296 T5161 T5159 arg1Of pathways,cellular
R4297 T5161 T5160 arg1Of pathways,signaling
R4298 T5165 T5164 arg1Of NF-κB,particular
R4299 T5165 T5166 arg1Of NF-κB,","
R4300 T5166 T5169 arg1Of ",",and
R4301 T5167 T5166 arg2Of TBK1/IRF3,","
R4302 T5169 T5163 arg2Of and,in
R4303 T5169 T5168 arg1Of and,","
R4304 T5172 T5171 arg2Of 23,[
R4305 T5173 T5171 arg3Of ],[
R4306 T5174 T5169 arg2Of –,and
R4307 T5174 T5170 arg1Of –,MAPK
R4308 T5174 T5171 arg1Of –,[
R4309 T5174 T5175 arg1Of –,[
R4310 T5176 T5175 arg2Of 27,[
R4311 T5177 T5175 arg3Of ],[
R4312 T5178 T5180 arg1Of We,determined
R4313 T5180 T5179 arg1Of determined,next
R4314 T5180 T5181 arg1Of determined,if
R4315 T5183 T5182 arg2Of NF-κB,disrupted
R4316 T5183 T5184 arg1Of NF-κB,","
R4317 T5184 T5187 arg1Of ",",and/or
R4318 T5185 T5184 arg2Of TBK1/IRF3,","
R4319 T5187 T5186 arg1Of and/or,","
R4320 T5187 T5190 arg1Of and/or,may
R4321 T5187 T5191 arg1Of and/or,underlie
R4322 T5189 T5187 arg2Of signaling,and/or
R4323 T5189 T5188 arg1Of signaling,MAPK
R4324 T5191 T5181 arg2Of underlie,if
R4325 T5191 T5190 arg2Of underlie,may
R4326 T5195 T5191 arg2Of production,underlie
R4327 T5195 T5192 arg1Of production,the
R4328 T5195 T5193 arg1Of production,impaired
R4329 T5195 T5194 arg1Of production,cytokine
R4330 T5195 T5196 arg1Of production,from
R4331 T5199 T5196 arg2Of BMDC,from
R4332 T5199 T5197 arg2Of BMDC,stimulated
R4333 T5199 T5198 arg1Of BMDC,Sharpin-deficient
R4334 T5201 T5200 arg1Of phosphorylation,Stimulus-induced
R4335 T5201 T5202 arg1Of phosphorylation,of
R4336 T5201 T5209 arg1Of phosphorylation,is
R4337 T5205 T5202 arg2Of kinase,of
R4338 T5205 T5203 arg1Of kinase,the
R4339 T5205 T5204 arg1Of kinase,IκB
R4340 T5205 T5206 arg1Of kinase,(
R4341 T5207 T5206 arg2Of IKK1/2,(
R4342 T5208 T5206 arg3Of ),(
R4343 T5209 T5216 arg1Of is,","
R4344 T5209 T5217 modOf is,allowing
R4345 T5212 T5209 arg2Of step,is
R4346 T5212 T5210 arg1Of step,an
R4347 T5212 T5211 arg1Of step,essential
R4348 T5212 T5213 arg1Of step,in
R4349 T5215 T5213 arg2Of signaling,in
R4350 T5215 T5214 arg1Of signaling,NF-κB
R4351 T5218 T5219 arg1Of phosphorylation,and
R4352 T5219 T5217 arg2Of and,allowing
R4353 T5219 T5222 arg1Of and,of
R4354 T5219 T5228 arg1Of and,release
R4355 T5221 T5219 arg2Of degradation,and
R4356 T5221 T5220 arg1Of degradation,proteasome-mediated
R4357 T5226 T5222 arg2Of IκBα,of
R4358 T5226 T5223 arg1Of IκBα,the
R4359 T5226 T5224 arg1Of IκBα,NF-κB
R4360 T5226 T5225 arg1Of IκBα,inhibitor
R4361 T5228 T5217 arg3Of release,allowing
R4362 T5228 T5227 arg1Of release,to
R4363 T5228 T5233 arg1Of release,into
R4364 T5232 T5228 arg2Of factors,release
R4365 T5232 T5229 arg1Of factors,the
R4366 T5232 T5230 arg1Of factors,NF-κB
R4367 T5232 T5231 arg1Of factors,transcription
R4368 T5235 T5233 arg2Of nucleus,into
R4369 T5235 T5234 arg1Of nucleus,the
R4370 T5237 T5236 arg1Of amount,The
R4371 T5237 T5238 arg1Of amount,of
R4372 T5237 T5249 arg1Of amount,following
R4373 T5237 T5258 arg1Of amount,was
R4374 T5237 T5260 arg2Of amount,decreased
R4375 T5240 T5239 arg2Of IKK1/2,phosphorylated
R4376 T5240 T5241 arg1Of IKK1/2,(
R4377 T5240 T5244 arg1Of IKK1/2,and
R4378 T5242 T5241 arg2Of p-IKK1/2,(
R4379 T5243 T5241 arg3Of ),(
R4380 T5244 T5238 arg2Of and,of
R4381 T5245 T5244 arg2Of IκBα,and
R4382 T5245 T5246 arg1Of IκBα,(
R4383 T5247 T5246 arg2Of p-IκBα,(
R4384 T5248 T5246 arg3Of ),(
R4385 T5250 T5251 arg1Of incubation,with
R4386 T5250 T5253 arg1Of incubation,or
R4387 T5252 T5251 arg2Of LPS,with
R4388 T5253 T5249 arg2Of or,following
R4389 T5257 T5253 arg2Of C,or
R4390 T5257 T5254 arg1Of C,poly
R4391 T5257 T5255 arg1Of C,I
R4392 T5257 T5256 arg1Of C,:
R4393 T5260 T5258 arg2Of decreased,was
R4394 T5260 T5259 arg1Of decreased,severely
R4395 T5260 T5261 arg1Of decreased,in
R4396 T5260 T5264 arg1Of decreased,as
R4397 T5263 T5261 arg2Of BMDC,in
R4398 T5263 T5262 arg1Of BMDC,cpdm
R4399 T5265 T5264 arg2Of compared,as
R4400 T5265 T5266 arg1Of compared,with
R4401 T5268 T5266 arg2Of controls,with
R4402 T5268 T5267 arg1Of controls,WT
R4403 T5268 T5269 arg1Of controls,(
R4404 T5270 T5269 arg2Of Fig.,(
R4405 T5270 T5271 arg1Of Fig.,6
R4406 T5272 T5269 arg3Of ),(
R4407 T5275 T5273 arg1Of BMDC,The
R4408 T5275 T5274 arg1Of BMDC,cpdm
R4409 T5275 T5276 arg1Of BMDC,exhibited
R4410 T5276 T5287 arg1Of exhibited,to
R4411 T5278 T5276 arg2Of levels,exhibited
R4412 T5278 T5277 arg1Of levels,similar
R4413 T5278 T5279 arg1Of levels,of
R4414 T5280 T5281 arg1Of TBK1,","
R4415 T5281 T5284 arg1Of ",",and
R4416 T5282 T5281 arg2Of ERK1/2,","
R4417 T5284 T5279 arg2Of and,of
R4418 T5284 T5283 arg1Of and,","
R4419 T5286 T5284 arg2Of phosphorylation,and
R4420 T5286 T5285 arg1Of phosphorylation,p38
R4421 T5288 T5287 arg2Of those,to
R4422 T5288 T5289 arg1Of those,of
R4423 T5291 T5289 arg2Of cells,of
R4424 T5291 T5290 arg1Of cells,WT
R4425 T5291 T5292 arg1Of cells,(
R4426 T5293 T5292 arg2Of Fig.,(
R4427 T5293 T5294 arg1Of Fig.,6
R4428 T5295 T5292 arg3Of ),(
R4429 T5297 T5296 arg1Of results,These
R4430 T5297 T5298 arg1Of results,indicate
R4431 T5301 T5300 arg1Of absence,the
R4432 T5301 T5302 arg1Of absence,of
R4433 T5301 T5305 arg1Of absence,decreased
R4434 T5301 T5309 arg1Of absence,did
R4435 T5301 T5311 arg1Of absence,affect
R4436 T5304 T5302 arg2Of SHARPIN,of
R4437 T5304 T5303 arg1Of SHARPIN,functional
R4438 T5305 T5308 arg1Of decreased,but
R4439 T5307 T5305 arg2Of activation,decreased
R4440 T5307 T5306 arg1Of activation,NF-κB
R4441 T5308 T5298 arg2Of but,indicate
R4442 T5308 T5299 arg1Of but,that
R4443 T5311 T5308 arg2Of affect,but
R4444 T5311 T5309 arg2Of affect,did
R4445 T5311 T5310 arg1Of affect,not
R4446 T5312 T5313 arg1Of TBK1/IRF3,","
R4447 T5313 T5316 arg1Of ",",and
R4448 T5314 T5313 arg2Of ERK1/2,","
R4449 T5316 T5311 arg2Of and,affect
R4450 T5316 T5315 arg1Of and,","
R4451 T5316 T5319 arg1Of and,in
R4452 T5318 T5316 arg2Of signaling,and
R4453 T5318 T5317 arg1Of signaling,p38
R4454 T5320 T5319 arg2Of BMDC,in
R4958 T6138 T6137 arg1Of immunogenicity,Th2-biased
R4959 T6138 T6139 arg1Of immunogenicity,of
R4960 T6142 T6139 arg2Of BMDC,of
R4961 T6142 T6140 arg2Of BMDC,stimulated
R4962 T6142 T6141 arg1Of BMDC,cpdm
R4963 T6146 T6143 arg1Of production,The
R4964 T6146 T6144 arg1Of production,defective
R4965 T6146 T6145 arg1Of production,IL12
R4966 T6146 T6147 arg1Of production,(
R4967 T6146 T6151 arg1Of production,and
R4968 T6149 T6147 arg2Of 3A,(
R4969 T6149 T6148 arg1Of 3A,Fig.
R4970 T6150 T6147 arg3Of ),(
R4971 T6151 T6161 arg1Of and,suggest
R4972 T6154 T6151 arg2Of profile,and
R4973 T6154 T6152 arg1Of profile,Th2-dominant
R4974 T6154 T6153 arg1Of profile,cytokine
R4975 T6154 T6155 arg1Of profile,in
R4976 T6157 T6155 arg2Of mice,in
R4977 T6157 T6156 arg1Of mice,cpdm
R4978 T6157 T6158 arg1Of mice,[
R4979 T6159 T6158 arg2Of 5,[
R4980 T6160 T6158 arg3Of ],[
R4981 T6164 T6163 arg1Of absence,the
R4982 T6164 T6165 arg1Of absence,of
R4983 T6164 T6167 arg1Of absence,affects
R4984 T6166 T6165 arg2Of SHARPIN,of
R4985 T6167 T6161 arg2Of affects,suggest
R4986 T6167 T6162 arg1Of affects,that
R4987 T6169 T6167 arg2Of ability,affects
R4988 T6169 T6168 arg1Of ability,the
R4989 T6169 T6170 arg1Of ability,of
R4990 T6169 T6173 modOf ability,to
R4991 T6172 T6170 arg2Of BMDC,of
R4992 T6172 T6171 arg1Of BMDC,cpdm
R4993 T6174 T6173 arg1Of induce,to
R4994 T6174 T6178 arg1Of induce,into
R4995 T6177 T6174 arg2Of differentiation,induce
R4996 T6177 T6175 arg1Of differentiation,T
R4997 T6177 T6176 arg1Of differentiation,cell
R4998 T6180 T6178 arg2Of cells,into
R4999 T6180 T6179 arg1Of cells,effector
R5000 T6181 T6182 arg1Of Co-culture,of
R5001 T6181 T6188 arg1Of Co-culture,with
R5002 T6181 T6199 arg1Of Co-culture,elicited
R5003 T6187 T6182 arg2Of cells,of
R5004 T6187 T6183 arg1Of cells,allogeneic
R5005 T6187 T6184 arg1Of cells,naïve
R5006 T6187 T6185 arg1Of cells,CD4+
R5007 T6187 T6186 arg1Of cells,T
R5008 T6190 T6189 arg1Of BMDC,WT
R5009 T6190 T6191 arg2Of BMDC,stimulated
R5010 T6190 T6194 arg1Of BMDC,or
R5011 T6191 T6192 arg1Of stimulated,with
R5012 T6193 T6192 arg2Of LPS,with
R5013 T6194 T6188 arg2Of or,with
R5014 T6198 T6194 arg2Of C,or
R5015 T6198 T6195 arg1Of C,poly
R5016 T6198 T6196 arg1Of C,I
R5017 T6198 T6197 arg1Of C,:
R5018 T6199 T6203 arg1Of elicited,","
R5019 T6199 T6204 arg1Of elicited,whereas
R5020 T6202 T6199 arg2Of production,elicited
R5021 T6202 T6200 arg1Of production,robust
R5022 T6202 T6201 arg1Of production,IFNγ
R5023 T6206 T6205 arg1Of concentration,the
R5024 T6206 T6207 arg1Of concentration,of
R5025 T6206 T6209 arg1Of concentration,in
R5026 T6206 T6214 arg1Of concentration,was
R5027 T6206 T6216 arg1Of concentration,lower
R5028 T6208 T6207 arg2Of IFNγ,of
R5029 T6213 T6209 arg2Of cultures,in
R5030 T6213 T6210 arg1Of cultures,cpdm
R5031 T6213 T6211 arg1Of cultures,BMDC-T
R5032 T6213 T6212 arg1Of cultures,cell
R5033 T6214 T6204 arg2Of was,whereas
R5034 T6214 T6217 arg1Of was,after
R5035 T6214 T6224 arg1Of was,","
R5036 T6214 T6225 modOf was,indicating
R5037 T6216 T6214 arg2Of lower,was
R5038 T6216 T6215 arg1Of lower,significantly
R5039 T6219 T6217 arg2Of stimulation,after
R5040 T6219 T6218 arg1Of stimulation,LPS
R5041 T6219 T6220 arg1Of stimulation,(
R5042 T6222 T6220 arg2Of 7A,(
R5043 T6222 T6221 arg1Of 7A,Fig.
R5044 T6223 T6220 arg3Of ),(
R5045 T6227 T6226 arg1Of Th1-polarizing,impaired
R5046 T6228 T6225 arg2Of abilities,indicating
R5047 T6228 T6227 arg1Of abilities,Th1-polarizing
R5048 T6228 T6229 arg1Of abilities,of
R5049 T6231 T6229 arg2Of BMDC,of
R5050 T6231 T6230 arg1Of BMDC,cpdm
R5051 T6232 T6234 arg1Of In,to
R5052 T6233 T6232 arg2Of addition,In
R5053 T6236 T6232 arg3Of agonists,In
R5054 T6236 T6235 arg1Of agonists,TLR3/4
R5055 T6241 T6238 arg1Of Pam3CYS,the
R5056 T6241 T6239 arg1Of Pam3CYS,TLR2
R5057 T6241 T6240 arg1Of Pam3CYS,ligand
R5058 T6241 T6242 arg1Of Pam3CYS,was
R5059 T6241 T6243 arg2Of Pam3CYS,used
R5060 T6243 T6232 arg1Of used,In
R5061 T6243 T6237 arg1Of used,","
R5062 T6243 T6242 arg2Of used,was
R5063 T6243 T6244 arg1Of used,since
R5064 T6245 T6246 arg1Of it,has
R5065 T6245 T6247 arg1Of it,been
R5066 T6245 T6248 arg2Of it,shown
R5067 T6245 T6250 arg1Of it,induce
R5068 T6248 T6244 arg2Of shown,since
R5069 T6248 T6246 arg2Of shown,has
R5070 T6248 T6247 arg2Of shown,been
R5071 T6250 T6248 arg3Of induce,shown
R5072 T6250 T6249 arg1Of induce,to
R5073 T6250 T6260 arg1Of induce,[
R5074 T6252 T6253 arg1Of Th1,and
R5075 T6254 T6253 arg2Of Th2,and
R5076 T6255 T6250 arg2Of responses,induce
R5077 T6255 T6251 arg1Of responses,both
R5078 T6255 T6252 arg1Of responses,Th1
R5079 T6255 T6254 arg1Of responses,Th2
R5080 T6255 T6256 arg1Of responses,[
R5081 T6255 T6259 arg1Of responses,–
R5082 T6257 T6256 arg2Of 28,[
R5083 T6258 T6256 arg3Of ],[
R5084 T6261 T6260 arg2Of 30,[
R5085 T6262 T6260 arg3Of ],[
R5086 T6265 T6263 arg1Of BMDC,Pam3CYS-matured
R5087 T6265 T6264 arg1Of BMDC,WT
R5088 T6265 T6266 arg1Of BMDC,induced
R5089 T6269 T6266 arg2Of production,induced
R5090 T6269 T6267 arg1Of production,robust
R5091 T6269 T6268 arg1Of production,IFNγ
R5092 T6269 T6270 arg1Of production,at
R5093 T6269 T6275 arg1Of production,than
R5094 T6273 T6272 arg1Of higher,significantly
R5095 T6274 T6270 arg2Of level,at
R5096 T6274 T6271 arg1Of level,a
R5097 T6274 T6273 arg1Of level,higher
R5098 T6277 T6275 arg2Of BMDC,than
R5099 T6277 T6276 arg1Of BMDC,cpdm
R5100 T6277 T6278 arg1Of BMDC,(
R5101 T6280 T6278 arg2Of 7A,(
R5102 T6280 T6279 arg1Of 7A,Fig.
R5103 T6281 T6278 arg3Of ),(
R5104 T6285 T6282 arg1Of differentiation,The
R5105 T6285 T6283 arg2Of differentiation,reduced
R5106 T6285 T6284 arg1Of differentiation,Th1
R5107 T6285 T6286 arg1Of differentiation,following
R5108 T6285 T6289 arg1Of differentiation,is
R5109 T6285 T6290 arg1Of differentiation,consistent
R5110 T6288 T6286 arg2Of stimulation,following
R5111 T6288 T6287 arg1Of stimulation,Pam3CYS
R5112 T6290 T6289 arg2Of consistent,is
R5113 T6290 T6291 arg1Of consistent,with
R5114 T6294 T6291 arg2Of report,with
R5115 T6294 T6292 arg1Of report,the
R5116 T6294 T6293 arg1Of report,recent
R5117 T6294 T6295 arg1Of report,of
R5118 T6294 T6299 arg1Of report,in
R5119 T6298 T6295 arg2Of production,of
R5120 T6298 T6296 arg2Of production,decreased
R5121 T6298 T6297 arg1Of production,IL12
R5122 T6301 T6299 arg2Of macrophages,in
R5123 T6301 T6300 arg1Of macrophages,cpdm
R5124 T6301 T6302 arg1Of macrophages,following
R5125 T6304 T6302 arg2Of stimulation,following
R5126 T6304 T6303 arg1Of stimulation,TLR2
R5127 T6304 T6305 arg1Of stimulation,[
R5128 T6306 T6305 arg2Of 31,[
R5129 T6307 T6305 arg3Of ],[
R5130 T6309 T6308 arg2Of contrast,In
R5131 T6312 T6311 arg1Of Th2-specific,more
R5132 T6314 T6312 arg1Of cytokine,Th2-specific
R5133 T6314 T6313 arg1Of cytokine,IL4
R5134 T6314 T6315 arg1Of cytokine,was
R5135 T6314 T6316 arg2Of cytokine,produced
R5136 T6316 T6308 arg1Of produced,In
R5137 T6316 T6310 arg1Of produced,","
R5138 T6316 T6315 arg2Of produced,was
R5139 T6316 T6317 arg1Of produced,in
R5140 T6316 T6330 arg1Of produced,","
R5141 T6321 T6317 arg2Of co-cultures,in
R5142 T6321 T6318 arg1Of co-cultures,the
R5143 T6321 T6319 arg1Of co-cultures,cpdm
R5144 T6321 T6320 arg1Of co-cultures,BMDC
R5145 T6321 T6322 arg1Of co-cultures,than
R5146 T6325 T6322 arg2Of control,than
R5147 T6325 T6323 arg1Of control,the
R5148 T6325 T6324 arg1Of control,WT
R5149 T6325 T6326 arg1Of control,(
R5150 T6328 T6326 arg2Of 7B,(
R5151 T6328 T6327 arg1Of 7B,Fig.
R5152 T6329 T6326 arg3Of ),(
R5153 T6331 T6316 arg3Of suggesting,produced
R5154 T6333 T6331 arg2Of immunogenicity,suggesting
R5155 T6333 T6332 arg1Of immunogenicity,Th2-skewed
R5156 T6333 T6334 arg1Of immunogenicity,of
R5157 T6336 T6334 arg2Of BMDC,of
R5158 T6336 T6335 arg1Of BMDC,cpdm
R5159 T6338 T6337 arg1Of production,The
R5160 T6338 T6339 arg1Of production,of
R5161 T6338 T6343 arg1Of production,following
R5162 T6338 T6349 arg1Of production,was
R5163 T6338 T6350 arg1Of production,similar
R5164 T6342 T6339 arg2Of IL17A,of
R5165 T6342 T6340 arg1Of IL17A,Th17-specific
R5166 T6342 T6341 arg1Of IL17A,cytokine
R5167 T6345 T6343 arg2Of stimulation,following
R5168 T6345 T6344 arg1Of stimulation,LPS
R5169 T6345 T6346 arg1Of stimulation,of
R5170 T6348 T6346 arg2Of cells,of
R5171 T6348 T6347 arg1Of cells,dendritic
R5172 T6349 T6351 arg1Of was,between
R5173 T6350 T6349 arg2Of similar,was
R5174 T6353 T6352 arg2Of WT,stimulated
R5175 T6353 T6354 arg1Of WT,and
R5176 T6354 T6351 arg2Of and,between
R5177 T6357 T6354 arg2Of cocultures,and
R5178 T6357 T6355 arg1Of cocultures,cpdm
R5179 T6357 T6356 arg1Of cocultures,BMDC
R5180 T6357 T6358 arg1Of cocultures,(
R5181 T6359 T6358 arg2Of data,(
R5182 T6359 T6361 arg2Of data,shown
R5183 T6361 T6360 arg1Of shown,not
R5184 T6362 T6358 arg3Of ),(
R5185 T6365 T6366 arg1Of distinct,Th1-
R5186 T6365 T6367 arg1Of distinct,and
R5187 T6368 T6367 arg2Of Th2-stimulating,and
R5188 T6369 T6363 arg2Of abilities,Despite
R5189 T6369 T6364 arg1Of abilities,the
R5190 T6369 T6365 arg1Of abilities,distinct
R5191 T6369 T6368 arg1Of abilities,Th2-stimulating
R5192 T6369 T6370 arg1Of abilities,of
R5193 T6371 T6372 arg1Of WT,and
R5194 T6373 T6372 arg2Of cpdm,and
R5195 T6374 T6370 arg2Of BMDC,of
R5196 T6374 T6371 arg1Of BMDC,WT
R5197 T6374 T6373 arg1Of BMDC,cpdm
R5198 T6376 T6377 arg1Of they,were
R5199 T6376 T6379 arg1Of they,effective
R5200 T6377 T6363 arg1Of were,Despite
R5201 T6377 T6375 arg1Of were,","
R5202 T6379 T6377 arg2Of effective,were
R5203 T6379 T6378 arg1Of effective,equally
R5204 T6379 T6380 arg1Of effective,in
R5205 T6382 T6380 arg2Of production,in
R5206 T6382 T6381 arg1Of production,IL2
R5207 T6382 T6383 arg1Of production,from
R5208 T6382 T6388 arg1Of production,for
R5209 T6382 T6394 arg1Of production,where
R5210 T6386 T6383 arg2Of co-cultures,from
R5211 T6386 T6384 arg1Of co-cultures,BMDC-T
R5212 T6386 T6385 arg1Of co-cultures,cell
R5213 T6388 T6387 arg1Of for,except
R5214 T6390 T6391 arg1Of I,:
R5215 T6392 T6391 arg2Of C,:
R5216 T6393 T6388 arg2Of stimulation,for
R5217 T6393 T6389 arg1Of stimulation,poly
R5218 T6393 T6390 arg1Of stimulation,I
R5219 T6393 T6392 arg1Of stimulation,C
R5220 T6396 T6395 arg1Of BMDC,WT
R5221 T6396 T6397 arg1Of BMDC,induced
R5222 T6397 T6394 arg2Of induced,where
R5223 T6399 T6397 arg2Of IL2,induced
R5224 T6399 T6398 arg1Of IL2,more
R5225 T6399 T6400 arg1Of IL2,than
R5226 T6402 T6400 arg2Of BMDC,than
R5227 T6402 T6401 arg1Of BMDC,cpdm
R5228 T6402 T6403 arg1Of BMDC,(
R5229 T6405 T6403 arg2Of 7C,(
R5230 T6405 T6404 arg1Of 7C,Fig.
R5231 T6406 T6403 arg3Of ),(
R5232 T6410 T6407 arg1Of capability,The
R5233 T6410 T6408 arg1Of capability,Th2-biased
R5234 T6410 T6409 arg1Of capability,stimulating
R5235 T6410 T6411 arg1Of capability,of
R5236 T6410 T6415 arg1Of capability,co-cultured
R5237 T6410 T6422 arg1Of capability,is
R5238 T6410 T6423 arg1Of capability,consistent
R5239 T6413 T6411 arg2Of BMDC,of
R5240 T6413 T6412 arg1Of BMDC,cpdm
R5241 T6415 T6414 arg2Of co-cultured,when
R5242 T6415 T6416 arg1Of co-cultured,with
R5243 T6421 T6416 arg2Of cells,with
R5244 T6421 T6417 arg1Of cells,allogeneic
R5245 T6421 T6418 arg1Of cells,naïve
R5246 T6421 T6419 arg1Of cells,CD4+
R5247 T6421 T6420 arg1Of cells,T
R5248 T6422 T6414 arg1Of is,when
R5249 T6423 T6422 arg2Of consistent,is
R5250 T6423 T6424 arg1Of consistent,with
R5251 T6428 T6424 arg2Of phenotype,with
R5252 T6428 T6425 arg1Of phenotype,the
R5253 T6428 T6426 arg1Of phenotype,Th2-dominant
R5254 T6428 T6427 arg1Of phenotype,cytokine
R5255 T6428 T6429 arg2Of phenotype,observed
R5256 T6429 T6430 arg1Of observed,in
R5257 T6432 T6430 arg2Of mice,in
R5258 T6432 T6431 arg1Of mice,cpdm
R6548 T7944 T7942 arg1Of report,The
R6549 T7944 T7943 arg1Of report,present
R6550 T7944 T7945 arg1Of report,showed
R6551 T7948 T7947 arg1Of mutations,null
R6552 T7948 T7949 arg1Of mutations,of
R6553 T7948 T7954 arg1Of mutations,did
R6554 T7948 T7956 arg1Of mutations,affect
R6558 T7953 T7952 arg1Of gene,Sharpin
R6559 T7956 T7945 arg2Of affect,showed
R6560 T7956 T7946 arg1Of affect,that
R6561 T7956 T7954 arg2Of affect,did
R6562 T7956 T7955 arg1Of affect,not
R6563 T7959 T7957 arg1Of distribution,the
R6564 T7959 T7958 arg1Of distribution,steady-state
R6565 T7959 T7960 arg1Of distribution,of
R6566 T7959 T7964 arg1Of distribution,nor
R6567 T7963 T7960 arg2Of subsets,of
R6568 T7963 T7961 arg1Of subsets,splenic
R6569 T7963 T7962 arg1Of subsets,DC
R6570 T7964 T7956 arg2Of nor,affect
R6571 T7966 T7967 arg1Of development,and
R6572 T7967 T7964 arg2Of and,nor
R6573 T7967 T7965 arg1Of and,the
R6574 T7967 T7969 arg1Of and,of
R6575 T7968 T7967 arg2Of phenotype,and
R6576 T7970 T7969 arg2Of BMDC,of
R6577 T7973 T7974 arg1Of loss,of
R6578 T7973 T7977 arg1Of loss,diminished
R6579 T7975 T7974 arg2Of SHARPIN,of
R6580 T7977 T7971 arg1Of diminished,However
R6581 T7977 T7972 arg1Of diminished,","
R6582 T7977 T7976 arg1Of diminished,significantly
R6583 T7979 T7977 arg2Of capacity,diminished
R6584 T7979 T7978 arg1Of capacity,the
R6585 T7979 T7980 arg1Of capacity,of
R6586 T7979 T7982 modOf capacity,to
R6587 T7981 T7980 arg2Of BMDC,of
R6588 T7983 T7982 arg1Of secrete,to
R6589 T7985 T7984 arg1Of cytokines,inflammatory
R6590 T7985 T7986 arg1Of cytokines,and
R6591 T7986 T7983 arg2Of and,secrete
R6592 T7988 T7986 arg2Of oxide,and
R6593 T7988 T7987 arg1Of oxide,nitric
R6594 T7992 T7989 arg1Of production,The
R6595 T7992 T7990 arg2Of production,attenuated
R6596 T7992 T7991 arg1Of production,cytokine
R6597 T7992 T7993 arg1Of production,was
R6598 T7992 T7995 arg1Of production,due
R6599 T7992 T8004 arg1Of production,can
R6600 T7992 T8005 arg1Of production,be
R6601 T7992 T8007 arg2Of production,explained
R6602 T7993 T7994 arg1Of was,not
R6603 T7993 T8003 arg1Of was,and
R6604 T7995 T7993 arg2Of due,was
R6605 T7995 T7996 arg1Of due,to
R6606 T7998 T7996 arg2Of presence,to
R6607 T7998 T7997 arg1Of presence,the
R6608 T7998 T7999 arg1Of presence,of
R6609 T8001 T7999 arg2Of inhibitors,of
R6610 T8001 T8000 arg1Of inhibitors,anti-inflammatory
R6611 T8003 T8002 arg1Of and,","
R6612 T8007 T8003 arg2Of explained,and
R6613 T8007 T8004 arg2Of explained,can
R6614 T8007 T8005 arg2Of explained,be
R6615 T8007 T8006 arg1Of explained,largely
R6616 T8007 T8008 arg1Of explained,by
R6617 T8010 T8008 arg2Of inactivation,by
R6618 T8010 T8009 arg1Of inactivation,selective
R6619 T8010 T8011 arg1Of inactivation,of
R6620 T8013 T8011 arg2Of signaling,of
R6621 T8013 T8012 arg1Of signaling,NF-κB
R6622 T8016 T8014 arg2Of BMDC,Stimulated
R6623 T8016 T8015 arg1Of BMDC,cpdm
R6624 T8016 T8017 arg1Of BMDC,exhibited
R6625 T8017 T8022 arg1Of exhibited,","
R6626 T8017 T8023 arg1Of exhibited,consistent
R6627 T8021 T8017 arg2Of capabilities,exhibited
R6628 T8021 T8018 arg1Of capabilities,Th2-biased
R6629 T8021 T8019 arg1Of capabilities,T
R6630 T8021 T8020 arg1Of capabilities,cell-polarizing
R6631 T8023 T8024 arg1Of consistent,with
R6632 T8028 T8024 arg2Of phenotype,with
R6633 T8028 T8025 arg1Of phenotype,the
R6634 T8028 T8026 arg1Of phenotype,Th2
R6635 T8028 T8027 arg1Of phenotype,cytokine-dominant
R6636 T8028 T8029 arg1Of phenotype,in
R6637 T8031 T8029 arg2Of mice,in
R6638 T8031 T8030 arg1Of mice,cpdm
R6639 T8035 T8034 arg1Of results,these
R6640 T8035 T8036 arg1Of results,indicate
R6641 T8036 T8032 arg1Of indicate,Together
R6642 T8036 T8033 arg1Of indicate,","
R6643 T8039 T8036 arg2Of role,indicate
R6644 T8039 T8037 arg1Of role,an
R6645 T8039 T8038 arg1Of role,indispensible
R6646 T8039 T8040 arg1Of role,of
R6647 T8041 T8040 arg2Of SHARPIN,of
R6648 T8041 T8042 arg1Of SHARPIN,in
R6649 T8043 T8042 arg2Of regulating,in
R6650 T8046 T8043 arg2Of functions,regulating
R6651 T8046 T8044 arg1Of functions,DC
R6652 T8046 T8045 arg1Of functions,immunological
R6653 T8046 T8049 arg2Of functions,of
R6654 T8046 T8050 arg1Of functions,which
R6655 T8048 T8049 arg1Of disruption,of
R6656 T8048 T8051 arg1Of disruption,may
R6657 T8048 T8052 arg1Of disruption,contribute
R6658 T8052 T8047 arg1Of contribute,","
R6659 T8052 T8051 arg2Of contribute,may
R6660 T8052 T8053 arg1Of contribute,to
R6661 T8055 T8053 arg2Of development,to
R6662 T8055 T8054 arg1Of development,the
R6663 T8055 T8056 arg1Of development,of
R6664 T8058 T8056 arg2Of diseases,of
R6665 T8058 T8057 arg1Of diseases,immune
R6666 T8060 T8061 arg1Of WT,and
R6667 T8062 T8061 arg2Of cpdm,and
R6668 T8063 T8060 arg1Of mice,WT
R6669 T8063 T8062 arg1Of mice,cpdm
R6670 T8063 T8064 arg1Of mice,are
R6671 T8064 T8059 arg2Of are,Since
R6672 T8067 T8064 arg2Of pathogen-free,are
R6673 T8067 T8065 arg1Of pathogen-free,both
R6674 T8067 T8066 arg1Of pathogen-free,specific
R6675 T8070 T8069 arg1Of nature,the
R6676 T8070 T8071 arg1Of nature,of
R6677 T8070 T8081 arg1Of nature,is
R6678 T8070 T8083 arg1Of nature,obvious
R6679 T8073 T8071 arg2Of trigger,of
R6680 T8073 T8072 arg1Of trigger,the
R6681 T8073 T8074 arg1Of trigger,of
R6682 T8073 T8078 arg1Of trigger,in
R6683 T8077 T8074 arg2Of inflammation,of
R6684 T8077 T8075 arg1Of inflammation,the
R6685 T8077 T8076 arg1Of inflammation,severe
R6686 T8080 T8078 arg2Of mice,in
R6687 T8080 T8079 arg1Of mice,cpdm
R6688 T8081 T8059 arg1Of is,Since
R6689 T8081 T8068 arg1Of is,","
R6690 T8081 T8082 arg1Of is,not
R6691 T8083 T8081 arg2Of obvious,is
R6692 T8087 T8084 arg1Of factor,One
R6693 T8087 T8085 arg1Of factor,such
R6694 T8087 T8086 arg1Of factor,initiating
R6695 T8087 T8088 arg1Of factor,could
R6696 T8087 T8089 arg1Of factor,be
R6697 T8089 T8088 arg2Of be,could
R6698 T8091 T8092 arg1Of apoptotic,and/or
R6699 T8093 T8092 arg2Of necrotic,and/or
R6700 T8094 T8089 arg2Of cells,be
R6701 T8094 T8090 arg1Of cells,endogenous
R6702 T8094 T8091 arg1Of cells,apoptotic
R6703 T8094 T8093 arg1Of cells,necrotic
R6704 T8094 T8095 arg1Of cells,that
R6705 T8094 T8096 arg1Of cells,can
R6706 T8094 T8097 arg1Of cells,release
R6707 T8097 T8096 arg2Of release,can
R6708 T8100 T8097 arg2Of patterns,release
R6709 T8100 T8098 arg1Of patterns,danger-associated
R6710 T8100 T8099 arg1Of patterns,molecular
R6711 T8100 T8101 arg1Of patterns,(
R6712 T8100 T8105 arg1Of patterns,launch
R6713 T8100 T8107 arg1Of patterns,amplify
R6714 T8102 T8101 arg2Of DAMPs,(
R6715 T8103 T8101 arg3Of ),(
R6716 T8105 T8106 arg1Of launch,and
R6717 T8106 T8097 arg3Of and,release
R6718 T8106 T8104 arg1Of and,to
R6719 T8106 T8111 arg1Of and,[
R6720 T8107 T8106 arg2Of amplify,and
R6721 T8110 T8105 arg2Of response,launch
R6722 T8110 T8107 arg2Of response,amplify
R6723 T8110 T8108 arg1Of response,an
R6724 T8110 T8109 arg1Of response,inflammatory
R6725 T8112 T8111 arg2Of 32,[
R6726 T8113 T8111 arg3Of ],[
R6727 T8116 T8114 arg1Of inflammation,Such
R6728 T8116 T8115 arg1Of inflammation,‘sterile’
R6729 T8116 T8117 arg1Of inflammation,could
R6730 T8116 T8118 arg1Of inflammation,be
R6731 T8116 T8119 arg2Of inflammation,initiated
R6732 T8116 T8121 arg1Of inflammation,take
R6733 T8119 T8118 arg2Of initiated,be
R6734 T8119 T8120 arg1Of initiated,and
R6735 T8120 T8117 arg2Of and,could
R6736 T8120 T8132 arg1Of and,","
R6737 T8120 T8134 modOf and,causing
R6738 T8121 T8120 arg2Of take,and
R6739 T8121 T8123 arg1Of take,in
R6740 T8122 T8121 arg2Of place,take
R6741 T8125 T8126 arg1Of organs,and
R6742 T8126 T8123 arg2Of and,in
R6743 T8126 T8124 arg1Of and,all
R6744 T8126 T8128 arg2Of and,affected
R6745 T8127 T8126 arg2Of tissues,and
R6746 T8128 T8129 arg1Of affected,in
R6747 T8131 T8129 arg2Of mice,in
R6748 T8131 T8130 arg1Of mice,cpdm
R6749 T8134 T8133 arg1Of causing,thus
R6750 T8137 T8134 arg2Of disorders,causing
R6751 T8137 T8135 arg1Of disorders,multi-organ
R6752 T8137 T8136 arg1Of disorders,inflammatory
R6753 T8139 T8138 arg1Of hypothesis,This
R6754 T8139 T8140 arg1Of hypothesis,is
R6755 T8139 T8141 arg2Of hypothesis,supported
R6756 T8141 T8140 arg2Of supported,is
R6757 T8145 T8141 arg1Of studies,supported
R6758 T8145 T8142 arg2Of studies,by
R6759 T8145 T8143 arg1Of studies,several
R6760 T8145 T8144 arg1Of studies,recent
R6761 T8146 T8147 arg1Of Fibroblasts,of
R6762 T8146 T8150 arg1Of Fibroblasts,are
R6763 T8146 T8152 arg1Of Fibroblasts,sensitive
R6764 T8149 T8147 arg2Of mice,of
R6765 T8149 T8148 arg1Of mice,cpdm
R6766 T8150 T8157 arg1Of are,and
R6767 T8152 T8150 arg2Of sensitive,are
R6768 T8152 T8151 arg1Of sensitive,highly
R6769 T8152 T8153 arg1Of sensitive,to
R6770 T8156 T8153 arg2Of death,to
R6771 T8156 T8154 arg1Of death,TNFα-induced
R6772 T8156 T8155 arg1Of death,cell
R6773 T8160 T8158 arg1Of phenotype,the
R6774 T8160 T8159 arg1Of phenotype,cpdm
R6775 T8160 T8161 arg1Of phenotype,can
R6776 T8160 T8162 arg1Of phenotype,be
R6777 T8160 T8164 arg2Of phenotype,rescued
R6778 T8164 T8157 arg2Of rescued,and
R6779 T8164 T8161 arg2Of rescued,can
R6780 T8164 T8162 arg2Of rescued,be
R6781 T8164 T8163 arg1Of rescued,partially
R6782 T8164 T8172 arg1Of rescued,","
R6783 T8164 T8173 arg1Of rescued,[
R6784 T8164 T8176 arg1Of rescued,","
R6785 T8164 T8177 modOf rescued,suggesting
R6786 T8166 T8164 arg1Of deletion,rescued
R6787 T8166 T8165 arg2Of deletion,by
R6788 T8166 T8167 arg1Of deletion,of
R6789 T8168 T8167 arg2Of TNF,of
R6790 T8168 T8169 arg1Of TNF,[
R6791 T8170 T8169 arg2Of 7,[
R6792 T8171 T8169 arg3Of ],[
R6793 T8174 T8173 arg2Of 8,[
R6794 T8175 T8173 arg3Of ],[
R6795 T8179 T8180 arg1Of deficiency,of
R6796 T8179 T8182 arg1Of deficiency,compromises
R6797 T8181 T8180 arg2Of SHARPIN,of
R6798 T8182 T8177 arg2Of compromises,suggesting
R6799 T8182 T8178 arg1Of compromises,that
R6800 T8182 T8186 arg1Of compromises,in
R6801 T8185 T8182 arg2Of mechanisms,compromises
R6802 T8185 T8183 arg1Of mechanisms,the
R6803 T8185 T8184 arg1Of mechanisms,anti-apoptotic
R6804 T8188 T8186 arg2Of mice,in
R6805 T8188 T8187 arg1Of mice,cpdm
R6806 T8188 T8189 arg1Of mice,resulting
R6807 T8189 T8190 arg1Of resulting,in
R6808 T8194 T8190 arg2Of disease,in
R6809 T8194 T8191 arg1Of disease,cell
R6810 T8194 T8192 arg1Of disease,death-induced
R6811 T8194 T8193 arg1Of disease,inflammatory
R6812 T8195 T8196 arg1Of Apoptosis,of
R6813 T8195 T8198 arg1Of Apoptosis,is
R6814 T8197 T8196 arg2Of keratinocytes,of
R6815 T8198 T8209 arg1Of is,[
R6816 T8198 T8212 arg1Of is,and
R6817 T8201 T8198 arg2Of feature,is
R6818 T8201 T8199 arg1Of feature,a
R6819 T8201 T8200 arg1Of feature,prominent
R6820 T8201 T8202 arg1Of feature,of
R6821 T8201 T8206 arg1Of feature,in
R6822 T8205 T8202 arg2Of lesions,of
R6823 T8205 T8203 arg1Of lesions,the
R6824 T8205 T8204 arg1Of lesions,skin
R6825 T8208 T8206 arg2Of mice,in
R6826 T8208 T8207 arg1Of mice,cpdm
R6827 T8210 T8209 arg2Of 33,[
R6828 T8211 T8209 arg3Of ],[
R6829 T8212 T8220 arg1Of and,[
R6830 T8213 T8214 arg1Of this,is
R6831 T8213 T8215 arg2Of this,mediated
R6832 T8215 T8212 arg2Of mediated,and
R6833 T8215 T8214 arg2Of mediated,is
R6834 T8219 T8215 arg1Of pathways,mediated
R6835 T8219 T8216 arg2Of pathways,by
R6836 T8219 T8217 arg1Of pathways,caspase-dependent
R6837 T8219 T8218 arg1Of pathways,mitochondrial
R6838 T8221 T8220 arg2Of 34,[
R6839 T8222 T8220 arg3Of ],[
R6840 T8322 T8319 arg1Of inflammation,the
R6841 T8224 T8225 arg1Of induced,and/or
R6842 T8226 T8225 arg2Of intrinsic,and/or
R6843 T8228 T8223 arg1Of cells,These
R6844 T8228 T8224 arg1Of cells,induced
R6845 T8228 T8226 arg1Of cells,intrinsic
R6846 T8228 T8227 arg1Of cells,apoptotic
R6847 T8228 T8229 arg1Of cells,can
R6848 T8228 T8230 arg1Of cells,release
R6849 T8230 T8229 arg2Of release,can
R6850 T8230 T8264 arg1Of release,","
R6851 T8230 T8265 arg1Of release,[
R6852 T8322 T8320 arg1Of inflammation,Th2-biased
R6853 T8232 T8230 arg2Of types,release
R6854 T8232 T8231 arg1Of types,various
R6855 T8232 T8233 arg1Of types,of
R6856 T8322 T8321 arg1Of inflammation,systemic
R6857 T8234 T8233 arg2Of DAMPs,of
R6858 T8234 T8235 arg1Of DAMPs,that
R6859 T8234 T8236 arg1Of DAMPs,exert
R6860 T8234 T8241 arg1Of DAMPs,activating
R6861 T8234 T8254 arg1Of DAMPs,","
R6862 T8234 T8256 arg1Of DAMPs,as
R6863 T8322 T8323 arg1Of inflammation,in
R6864 T8236 T8240 arg1Of exert,by
R6865 T8236 T8250 arg1Of exert,","
R6866 T8236 T8251 arg1Of exert,[
R6867 T8322 T8326 arg1Of inflammation,is
R6868 T8322 T8327 arg2Of inflammation,caused
R6869 T8239 T8236 arg2Of properties,exert
R6870 T8239 T8237 arg1Of properties,their
R6871 T8239 T8238 arg1Of properties,pro-inflammatory
R6872 T8325 T8323 arg2Of mice,in
R6873 T8241 T8240 arg2Of activating,by
R6874 T8241 T8243 arg1Of activating,through
R6875 T8242 T8241 arg2Of DC,activating
R6876 T8325 T8324 arg1Of mice,cpdm
R6877 T8246 T8243 arg2Of receptors,through
R6878 T8246 T8244 arg1Of receptors,pattern
R6879 T8246 T8245 arg1Of receptors,recognition
R6880 T8246 T8247 arg1Of receptors,[
R6881 T8248 T8247 arg2Of 32,[
R6882 T8327 T8317 arg2Of caused,suggests
R6883 T8249 T8247 arg3Of ],[
R6884 T8252 T8251 arg2Of 35,[
R6885 T8327 T8318 arg1Of caused,that
R6886 T8253 T8251 arg3Of ],[
R6887 T8327 T8326 arg2Of caused,is
R6888 T8331 T8327 arg1Of production,caused
R6889 T8256 T8255 arg1Of as,such
R6890 T8257 T8256 arg2Of HMGB1,as
R6891 T8257 T8258 arg2Of HMGB1,recognized
R6892 T8260 T8258 arg1Of TLR2/4,recognized
R6893 T8260 T8259 arg2Of TLR2/4,by
R6894 T8260 T8261 arg1Of TLR2/4,[
R6895 T8262 T8261 arg2Of 36,[
R6896 T8331 T8328 arg2Of production,by
R6897 T8263 T8261 arg3Of ],[
R6898 T8266 T8265 arg2Of 37,[
R6899 T8331 T8329 arg2Of production,reduced
R6900 T8267 T8265 arg3Of ],[
R6901 T8268 T8269 arg1Of Consistent,with
R6902 T8331 T8330 arg1Of production,IL12P70
R6903 T8331 T8332 arg1Of production,from
R6904 T8333 T8332 arg2Of DC,from
R6905 T8274 T8269 arg2Of response,with
R6906 T8274 T8270 arg1Of response,the
R6907 T8274 T8271 arg1Of response,impaired
R6908 T8274 T8272 arg1Of response,Th1
R6909 T8274 T8273 arg1Of response,immune
R6910 T8274 T8275 arg1Of response,in
R6911 T8335 T8334 arg1Of importance,The
R6912 T8277 T8275 arg2Of mice,in
R6913 T8277 T8276 arg1Of mice,cpdm
R6914 T8277 T8278 arg1Of mice,[
R6915 T8279 T8278 arg2Of 5,[
R6916 T8280 T8278 arg3Of ],[
R6917 T8335 T8336 arg1Of importance,of
R6918 T8335 T8338 arg1Of importance,in
R6919 T8335 T8345 arg1Of importance,in
R6920 T8283 T8282 arg1Of BMDC,stimulated
R6921 T8283 T8285 arg1Of BMDC,polarized
R6922 T8283 T8291 arg1Of BMDC,supported
R6923 T8335 T8347 arg1Of importance,was
R6924 T8285 T8289 arg1Of polarized,but
R6925 T8335 T8349 arg2Of importance,demonstrated
R6926 T8337 T8336 arg2Of IL12P70,of
R6927 T8287 T8285 arg2Of differentiation,polarized
R6928 T8287 T8286 arg1Of differentiation,Th1
R6929 T8289 T8268 arg1Of but,Consistent
R6930 T8289 T8281 arg1Of but,","
R6931 T8289 T8284 arg1Of but,weakly
R6932 T8289 T8288 arg1Of but,","
R6933 T8291 T8289 arg2Of supported,but
R6934 T8291 T8290 arg1Of supported,strongly
R6935 T8339 T8338 arg2Of regulating,in
R6936 T8293 T8291 arg2Of development,supported
R6937 T8293 T8292 arg1Of development,the
R6938 T8293 T8294 arg1Of development,of
R6939 T8297 T8294 arg2Of cells,of
R6940 T8297 T8295 arg1Of cells,Th2
R6941 T8297 T8296 arg1Of cells,effector
R6942 T8298 T8299 arg1Of Combined,with
R6943 T8340 T8341 arg1Of Th1,and
R6944 T8342 T8341 arg2Of Th2,and
R6945 T8302 T8299 arg2Of effect,with
R6946 T8302 T8300 arg1Of effect,the
R6947 T8302 T8301 arg1Of effect,dramatic
R6948 T8302 T8303 arg1Of effect,of
R6949 T8302 T8306 arg1Of effect,on
R6950 T8305 T8303 arg2Of treatment,of
R6951 T8305 T8304 arg1Of treatment,IL12
R6952 T8308 T8306 arg2Of phenotype,on
R6953 T8308 T8307 arg1Of phenotype,the
R6954 T8308 T8309 arg1Of phenotype,of
R6955 T8344 T8339 arg2Of responses,regulating
R6956 T8311 T8309 arg2Of mice,of
R6957 T8311 T8310 arg1Of mice,these
R6958 T8311 T8312 arg1Of mice,[
R6959 T8313 T8312 arg2Of 5,[
R6960 T8314 T8312 arg3Of ],[
R6961 T8344 T8340 arg1Of responses,Th1
R6962 T8316 T8298 arg2Of this,Combined
R6963 T8316 T8317 arg1Of this,suggests
R6964 T8344 T8342 arg1Of responses,Th2
R6965 T8317 T8298 modOf suggests,Combined
R6966 T8317 T8315 arg1Of suggests,","
R6967 T8344 T8343 arg1Of responses,immune
R6968 T8346 T8345 arg2Of mice,in
R6969 T8349 T8347 arg2Of demonstrated,was
R6970 T8416 T8405 arg1Of C,NF-κB
R6971 T8416 T8417 arg1Of C,stimulated
R6972 T8349 T8348 arg1Of demonstrated,clearly
R6973 T8417 T8420 arg1Of stimulated,compared
R6974 T8349 T8350 arg1Of demonstrated,in
R6975 T8417 T8424 arg1Of stimulated,","
R6976 T8417 T8425 arg1Of stimulated,while
R6977 T8349 T8363 arg1Of demonstrated,through
R6978 T8419 T8417 arg2Of BMDC,stimulated
R6979 T8419 T8418 arg1Of BMDC,cpdm
R6980 T8349 T8369 arg1Of demonstrated,[
R6981 T8421 T8420 arg2Of to,compared
R6982 T8349 T8372 arg1Of demonstrated,–
R6983 T8349 T8373 arg1Of demonstrated,[
R6984 T8423 T8421 arg2Of type,to
R6985 T8423 T8422 arg1Of type,wild
R6986 T8350 T8362 arg1Of in,and
R6987 T8426 T8427 arg1Of activation,of
R6988 T8426 T8434 arg1Of activation,was
R6989 T8426 T8437 arg1Of activation,indicating
R6990 T8351 T8352 arg1Of IL12P35-,and
R6991 T8428 T8429 arg1Of TBK1/IRF3,","
R6992 T8429 T8432 arg1Of ",",or
R6993 T8430 T8429 arg2Of ERK1/2,","
R6994 T8353 T8352 arg2Of IL12P40-deficient,and
R6995 T8432 T8427 arg2Of or,of
R6996 T8432 T8431 arg1Of or,","
R6997 T8433 T8432 arg2Of p38,or
R6998 T8437 T8425 arg2Of indicating,while
R6999 T8437 T8434 arg2Of indicating,was
R7000 T8437 T8435 arg1Of indicating,not
R7001 T8437 T8436 arg1Of indicating,","
R7002 T8354 T8350 arg2Of mice,in
R7003 T8441 T8439 arg2Of signaling,disrupted
R7004 T8441 T8440 arg1Of signaling,NF-κB
R7005 T8441 T8442 arg1Of signaling,in
R7006 T8441 T8445 arg1Of signaling,is
R7007 T8441 T8446 arg1Of signaling,responsible
R7008 T8354 T8351 arg1Of mice,IL12P35-
R7009 T8444 T8442 arg2Of DC,in
R7010 T8444 T8443 arg1Of DC,cpdm
R7011 T8445 T8437 arg2Of is,indicating
R7012 T8445 T8438 arg1Of is,that
R7013 T8354 T8353 arg1Of mice,IL12P40-deficient
R7014 T8446 T8445 arg2Of responsible,is
R7015 T8446 T8447 arg1Of responsible,for
R7016 T8354 T8355 arg1Of mice,[
R7017 T8354 T8358 arg1Of mice,","
R7018 T8354 T8359 arg1Of mice,[
R7019 T8356 T8355 arg2Of 38,[
R7020 T8451 T8447 arg2Of expression,for
R7021 T8451 T8448 arg1Of expression,the
R7022 T8451 T8449 arg1Of expression,defective
R7023 T8451 T8450 arg1Of expression,cytokine
R7024 T8453 T8452 arg1Of results,These
R7025 T8453 T8454 arg1Of results,seem
R7026 T8453 T8456 arg1Of results,contradict
R7027 T8357 T8355 arg3Of ],[
R7028 T8456 T8454 arg2Of contradict,seem
R7029 T8456 T8455 arg1Of contradict,to
R7030 T8360 T8359 arg2Of 39,[
R7031 T8459 T8456 arg2Of study,contradict
R7032 T8459 T8457 arg1Of study,a
R7033 T8459 T8458 arg1Of study,recent
R7034 T8459 T8460 arg1Of study,that
R7035 T8459 T8461 arg1Of study,found
R7036 T8461 T8473 arg1Of found,[
R7037 T8361 T8359 arg3Of ],[
R7038 T8463 T8461 arg2Of levels,found
R7039 T8463 T8462 arg2Of levels,increased
R7040 T8463 T8464 arg1Of levels,of
R7041 T8363 T8362 arg2Of through,and
R7042 T8466 T8465 arg1Of activation,NF-κB
R7043 T8466 T8467 arg1Of activation,and
R7044 T8467 T8464 arg2Of and,of
R7045 T8467 T8470 arg1Of and,in
R7046 T8469 T8467 arg2Of transcription,and
R7047 T8469 T8468 arg1Of transcription,IL1
R7048 T8365 T8363 arg2Of studies,through
R7049 T8472 T8470 arg2Of mice,in
R7050 T8472 T8471 arg1Of mice,cpdm
R7051 T8474 T8473 arg2Of 43,[
R7052 T8475 T8473 arg3Of ],[
R7053 T8477 T8476 arg1Of difference,This
R7054 T8477 T8478 arg1Of difference,can
R7055 T8477 T8480 arg1Of difference,be
R7056 T8477 T8481 arg2Of difference,attributed
R7057 T8477 T8494 arg1Of difference,may
R7058 T8477 T8495 arg1Of difference,point
R7059 T8365 T8364 arg1Of studies,clinical
R7060 T8365 T8366 arg1Of studies,in
R7061 T8481 T8478 arg2Of attributed,can
R7062 T8481 T8479 arg1Of attributed,probably
R7063 T8481 T8480 arg2Of attributed,be
R7064 T8481 T8482 arg1Of attributed,to
R7065 T8481 T8493 arg1Of attributed,and
R7066 T8368 T8366 arg2Of patients,in
R7067 T8485 T8486 arg1Of cells,and
R7068 T8486 T8482 arg2Of and,to
R7069 T8486 T8483 arg1Of and,the
R7070 T8486 T8484 arg1Of and,different
R7071 T8486 T8488 arg2Of and,used
R7072 T8487 T8486 arg2Of tissues,and
R7073 T8488 T8489 arg1Of used,in
R7074 T8368 T8367 arg1Of patients,human
R7075 T8370 T8369 arg2Of 40,[
R7076 T8491 T8489 arg2Of studies,in
R7077 T8491 T8490 arg1Of studies,these
R7078 T8493 T8492 arg1Of and,","
R7079 T8371 T8369 arg3Of ],[
R7080 T8495 T8493 arg2Of point,and
R7081 T8495 T8494 arg2Of point,may
R7082 T8495 T8496 arg1Of point,to
R7083 T8374 T8373 arg2Of 42,[
R7084 T8497 T8498 arg1Of cell-,and
R7085 T8499 T8498 arg2Of tissue-,and
R7086 T8375 T8373 arg3Of ],[
R7087 T8502 T8496 arg2Of functions,to
R7088 T8502 T8497 arg1Of functions,cell-
R7089 T8502 T8499 arg1Of functions,tissue-
R7090 T8502 T8500 arg1Of functions,type
R7091 T8502 T8501 arg1Of functions,specific
R7092 T8502 T8503 arg1Of functions,of
R7093 T8504 T8503 arg2Of SHARPIN,of
R7094 T8505 T8506 arg1Of This,is
R7095 T8505 T8507 arg1Of This,consistent
R7096 T8376 T8377 arg1Of NF-κB,","
R7097 T8377 T8380 arg1Of ",",and
R7098 T8506 T8517 arg1Of is,[
R7099 T8507 T8506 arg2Of consistent,is
R7100 T8507 T8508 arg1Of consistent,with
R7101 T8378 T8377 arg2Of TBK/IRF3,","
R7102 T8510 T8508 arg2Of reports,with
R7103 T8510 T8509 arg1Of reports,recent
R7104 T8510 T8511 arg1Of reports,of
R7105 T8380 T8379 arg1Of and,","
R7106 T8380 T8383 arg1Of and,are
R7107 T8382 T8380 arg2Of signaling,and
R7108 T8382 T8381 arg1Of signaling,MAPK
R7109 T8383 T8392 arg1Of are,:
R7110 T8513 T8511 arg2Of effects,of
R7111 T8513 T8512 arg1Of effects,tissue-specific
R7112 T8513 T8514 arg1Of effects,of
R7113 T8385 T8383 arg2Of pathways,are
R7114 T8516 T8514 arg2Of signaling,of
R7115 T8516 T8515 arg1Of signaling,NF-κB
R7116 T8518 T8517 arg2Of 44,[
R7117 T8519 T8517 arg3Of ],[
R7118 T8385 T8384 arg1Of pathways,important
R7119 T8385 T8386 arg2Of pathways,activated
R7120 T8521 T8520 arg1Of activation,Ubiquitous
R7121 T8521 T8522 arg1Of activation,of
R7122 T8521 T8524 arg1Of activation,by
R7123 T8521 T8535 arg1Of activation,results
R7124 T8521 T8540 arg1Of activation,consistent
R7125 T8388 T8389 arg1Of LPS,or
R7126 T8523 T8522 arg2Of NF-κB,of
R7127 T8525 T8524 arg2Of removing,by
R7128 T8526 T8525 arg2Of inhibitors,removing
R7129 T8526 T8528 arg1Of inhibitors,as
R7130 T8389 T8386 arg1Of or,activated
R7131 T8528 T8527 arg1Of as,such
R7132 T8529 T8530 arg1Of A20,and
R7133 T8530 T8528 arg2Of and,as
R7134 T8530 T8532 arg1Of and,through
R7135 T8389 T8387 arg2Of or,by
R7136 T8531 T8530 arg2Of ITCH,and
R7137 T8391 T8389 arg2Of I,or
R7138 T8534 T8532 arg2Of manipulation,through
R7139 T8534 T8533 arg1Of manipulation,genetic
R7140 T8535 T8536 arg1Of results,in
R7141 T8535 T8539 arg1Of results,","
R7142 T8535 T8540 modOf results,consistent
R7143 T8391 T8390 arg1Of I,poly
R7144 T8535 T8555 arg1Of results,","
R7145 T8535 T8556 arg1Of results,[
R7146 T8393 T8394 arg1Of C,and
R7147 T8538 T8536 arg2Of inflammation,in
R7148 T8538 T8537 arg1Of inflammation,widespread
R7149 T8540 T8541 arg1Of consistent,with
R7150 T8394 T8396 arg1Of and,of
R7151 T8543 T8541 arg2Of role,with
R7152 T8543 T8542 arg1Of role,the
R7153 T8543 T8544 arg1Of role,of
R7154 T8543 T8546 arg1Of role,in
R7155 T8545 T8544 arg2Of NF-κB,of
R7156 T8394 T8399 arg1Of and,may
R7157 T8394 T8400 arg1Of and,lead
R7158 T8548 T8546 arg2Of production,in
R7159 T8548 T8547 arg1Of production,the
R7160 T8548 T8549 arg1Of production,of
R7161 T8395 T8394 arg2Of disruption,and
R7162 T8551 T8549 arg2Of mediators,of
R7163 T8551 T8550 arg1Of mediators,pro-inflammatory
R7164 T8551 T8552 arg1Of mediators,[
R7165 T8398 T8396 arg2Of pathway,of
R7166 T8553 T8552 arg2Of 45,[
R7167 T8554 T8552 arg3Of ],[
R7168 T8398 T8397 arg1Of pathway,either
R7169 T8557 T8556 arg2Of 46,[
R7170 T8400 T8392 arg2Of lead,:
R7171 T8558 T8556 arg3Of ],[
R7172 T8400 T8399 arg2Of lead,may
R7173 T8400 T8401 arg1Of lead,to
R7174 T8562 T8561 arg1Of inhibition,selective
R7175 T8562 T8563 arg1Of inhibition,of
R7176 T8562 T8566 arg1Of inhibition,in
R7177 T8562 T8577 arg1Of inhibition,results
R7178 T8565 T8563 arg2Of activation,of
R7179 T8565 T8564 arg1Of activation,NF-κB
R7180 T8404 T8401 arg2Of expression,to
R7181 T8568 T8566 arg2Of cells,in
R7182 T8568 T8567 arg1Of cells,parenchymal
R7183 T8568 T8569 arg1Of cells,of
R7184 T8571 T8572 arg1Of skin,","
R7185 T8572 T8575 arg1Of ",",and
R7186 T8404 T8402 arg2Of expression,decreased
R7187 T8573 T8572 arg2Of liver,","
R7188 T8404 T8403 arg1Of expression,cytokine
R7189 T8575 T8569 arg2Of and,of
R7190 T8575 T8570 arg1Of and,the
R7191 T8575 T8574 arg1Of and,","
R7192 T8576 T8575 arg2Of intestine,and
R7193 T8405 T8407 modOf NF-κB,was
R7194 T8577 T8559 arg1Of results,However
R7195 T8577 T8560 arg1Of results,","
R7196 T8577 T8578 arg1Of results,in
R7197 T8577 T8590 arg1Of results,[
R7198 T8580 T8578 arg2Of inflammation,in
R7199 T8580 T8579 arg1Of inflammation,chronic
R7200 T8580 T8581 arg2Of inflammation,driven
R7201 T8580 T8589 arg1Of inflammation,–
R7202 T8581 T8586 arg1Of driven,[
R7203 T8405 T8415 arg1Of NF-κB,:
R7204 T8585 T8581 arg1Of leukocytes,driven
R7205 T8585 T8582 arg2Of leukocytes,by
R7206 T8585 T8583 arg1Of leukocytes,NF-κB
R7207 T8585 T8584 arg1Of leukocytes,competent
R7208 T8406 T8407 arg1Of activation,was
R7209 T8587 T8586 arg2Of 47,[
R7210 T8588 T8586 arg3Of ],[
R7211 T8406 T8409 arg2Of activation,inhibited
R7212 T8409 T8407 arg2Of inhibited,was
R7213 T8591 T8590 arg2Of 50,[
R7214 T8592 T8590 arg3Of ],[
R7215 T8593 T8594 arg1Of This,indicates
R7216 T8409 T8408 arg1Of inhibited,selectively
R7217 T8409 T8410 arg1Of inhibited,in
R7218 T8597 T8596 arg1Of balance,a
R7219 T8597 T8598 arg1Of balance,between
R7220 T8597 T8613 arg1Of balance,is
R7221 T8597 T8614 arg1Of balance,critical
R7222 T8411 T8412 arg1Of LPS,and
R7223 T8601 T8599 arg1Of role,the
R7224 T8601 T8600 arg1Of role,pro-inflammatory
R7225 T8601 T8602 arg1Of role,of
R7226 T8601 T8604 arg1Of role,in
R7227 T8601 T8606 arg1Of role,and
R7228 T8603 T8602 arg2Of NF-κB,of
R7229 T8412 T8410 arg2Of and,in
R7230 T8605 T8604 arg2Of leukocytes,in
R7231 T8606 T8598 arg2Of and,between
R7232 T8414 T8412 arg2Of I,and
R7233 T8609 T8606 arg2Of role,and
R7234 T8609 T8607 arg1Of role,the
R7235 T8609 T8608 arg1Of role,anti-inflammatory
R7236 T8609 T8610 arg1Of role,in
R7237 T8414 T8413 arg1Of I,poly
R7238 T8709 T8707 arg1Of pathway,The
R7239 T8709 T8708 arg1Of pathway,non-canonical
R7240 T8612 T8610 arg2Of cells,in
R7241 T8612 T8611 arg1Of cells,parenchymal
R7242 T8613 T8594 arg2Of is,indicates
R7243 T8613 T8595 arg1Of is,that
R7244 T8709 T8710 arg1Of pathway,(
R7245 T8614 T8613 arg2Of critical,is
R7246 T8614 T8615 arg1Of critical,in
R7247 T8709 T8717 arg1Of pathway,appears
R7248 T8709 T8719 arg1Of pathway,be
R7249 T8617 T8615 arg2Of maintenance,in
R7250 T8617 T8616 arg1Of maintenance,the
R7251 T8617 T8618 arg1Of maintenance,of
R7252 T8709 T8720 arg1Of pathway,intact
R7253 T8712 T8710 arg2Of processing,(
R7254 T8620 T8618 arg2Of homeostasis,of
R7255 T8620 T8619 arg1Of homeostasis,tissue
R7256 T8621 T8622 arg1Of Experiments,with
R7257 T8621 T8636 arg1Of Experiments,will
R7258 T8621 T8637 arg1Of Experiments,help
R7259 T8621 T8639 arg1Of Experiments,elucidate
R7260 T8623 T8622 arg2Of mice,with
R7261 T8623 T8624 arg1Of mice,with
R7262 T8625 T8626 arg1Of cell-,and
R7263 T8712 T8711 arg1Of processing,p100
R7264 T8627 T8626 arg2Of tissue-specific,and
R7265 T8712 T8713 modOf processing,to
R7266 T8628 T8624 arg2Of deletion,with
R7267 T8628 T8625 arg1Of deletion,cell-
R7268 T8628 T8627 arg1Of deletion,tissue-specific
R7269 T8628 T8629 arg1Of deletion,of
R7270 T8714 T8713 arg1Of produce,to
R7271 T8630 T8629 arg2Of Sharpin,of
R7272 T8630 T8631 arg1Of Sharpin,","
R7273 T8630 T8633 arg1Of Sharpin,under
R7274 T8633 T8632 arg1Of under,currently
R7275 T8715 T8714 arg2Of p52,produce
R7276 T8634 T8633 arg2Of way,under
R7277 T8716 T8710 arg3Of ),(
R7278 T8637 T8635 arg1Of help,","
R7279 T8637 T8636 arg2Of help,will
R7280 T8639 T8637 arg2Of elucidate,help
R7281 T8639 T8638 arg1Of elucidate,to
R7282 T8719 T8717 arg2Of be,appears
R7283 T8641 T8639 arg2Of role,elucidate
R7284 T8641 T8640 arg1Of role,the
R7285 T8641 T8642 arg1Of role,of
R7286 T8641 T8644 arg1Of role,in
R7287 T8643 T8642 arg2Of SHARPIN,of
R7288 T8645 T8644 arg2Of inflammation,in
R7289 T8719 T8718 arg1Of be,to
R7290 T8719 T8731 modOf be,suggesting
R7291 T8720 T8719 arg2Of intact,be
R7292 T8649 T8646 arg2Of activation,Despite
R7293 T8649 T8647 arg1Of activation,defective
R7294 T8649 T8648 arg1Of activation,NF-κB
R7295 T8649 T8650 arg1Of activation,in
R7296 T8650 T8651 arg1Of in,the
R7297 T8650 T8653 arg1Of in,of
R7298 T8652 T8650 arg2Of absence,in
R7299 T8720 T8721 arg1Of intact,in
R7300 T8655 T8650 arg3Of expression,in
R7301 T8655 T8654 arg1Of expression,Sharpin
R7302 T8723 T8721 arg2Of cells,in
R7303 T8657 T8658 arg1Of there,was
R7304 T8658 T8646 arg1Of was,Despite
R7305 T8658 T8656 arg1Of was,","
R7306 T8658 T8662 arg1Of was,in
R7307 T8661 T8658 arg2Of change,was
R7308 T8661 T8659 arg1Of change,no
R7309 T8661 T8660 arg1Of change,significant
R7310 T8723 T8722 arg1Of cells,SHARPIN-deficient
R7311 T8723 T8724 arg1Of cells,[
R7312 T8665 T8663 arg1Of populations,splenic
R7313 T8665 T8664 arg1Of populations,DC
R7314 T8665 T8666 arg1Of populations,or
R7315 T8666 T8662 arg2Of or,in
R7316 T8723 T8727 arg1Of cells,","
R7317 T8667 T8666 arg2Of expression,or
R7318 T8667 T8668 arg1Of expression,of
R7319 T8667 T8671 arg1Of expression,on
R7320 T8723 T8728 arg1Of cells,[
R7321 T8725 T8724 arg2Of 7,[
R7322 T8670 T8668 arg2Of molecules,of
R7323 T8670 T8669 arg1Of molecules,co-stimulatory
R7324 T8672 T8671 arg2Of BMDC,on
R7325 T8726 T8724 arg3Of ],[
R7326 T8675 T8673 arg1Of subunits,Different
R7327 T8675 T8674 arg1Of subunits,NF-κB
R7328 T8675 T8676 arg2Of subunits,involved
R7329 T8675 T8688 arg1Of subunits,have
R7330 T8675 T8692 arg1Of subunits,control
R7331 T8729 T8728 arg2Of 9,[
R7332 T8676 T8677 arg1Of involved,in
R7333 T8679 T8680 arg1Of canonical,and
R7334 T8730 T8728 arg3Of ],[
R7335 T8681 T8680 arg2Of non-canonical,and
R7336 T8682 T8677 arg2Of branches,in
R7337 T8682 T8678 arg1Of branches,the
R7338 T8682 T8679 arg1Of branches,canonical
R7339 T8682 T8681 arg1Of branches,non-canonical
R7340 T8682 T8683 arg1Of branches,of
R7341 T8736 T8733 arg1Of p52/RELB,the
R7342 T8687 T8683 arg2Of pathway,of
R7343 T8687 T8684 arg1Of pathway,the
R7344 T8687 T8685 arg1Of pathway,NF-κB
R7345 T8687 T8686 arg1Of pathway,signaling
R7346 T8688 T8691 modOf have,to
R7347 T8688 T8703 arg1Of have,","
R7348 T8688 T8704 arg1Of have,[
R7349 T8690 T8688 arg2Of functions,have
R7350 T8690 T8689 arg1Of functions,distinct
R7351 T8692 T8691 arg1Of control,to
R7352 T8694 T8692 arg2Of aspects,control
R7353 T8694 T8693 arg1Of aspects,specific
R7354 T8694 T8695 arg1Of aspects,of
R7355 T8736 T8734 arg1Of p52/RELB,NF-κB
R7356 T8697 T8696 arg1Of development,DC
R7357 T8697 T8698 arg1Of development,and
R7358 T8698 T8695 arg2Of and,of
R7359 T8736 T8735 arg1Of p52/RELB,heterodimer
R7360 T8699 T8698 arg2Of function,and
R7361 T8699 T8700 arg1Of function,[
R7362 T8736 T8737 arg1Of p52/RELB,is
R7363 T8701 T8700 arg2Of 51,[
R7364 T8736 T8738 arg1Of p52/RELB,sufficient
R7365 T8702 T8700 arg3Of ],[
R7366 T8737 T8731 arg2Of is,suggesting
R7367 T8737 T8732 arg1Of is,that
R7368 T8705 T8704 arg2Of 52,[
R7369 T8706 T8704 arg3Of ],[
R7370 T8737 T8739 modOf is,to
R7371 T8738 T8737 arg2Of sufficient,is
R7372 T8740 T8739 arg1Of maintain,to
R7373 T8743 T8740 arg2Of regulation,maintain
R7374 T8804 T8802 arg1Of activation,defective
R7375 T8804 T8803 arg1Of activation,NF-κB
R7376 T8804 T8805 arg1Of activation,is
R7377 T8805 T8800 arg2Of is,suggesting
R7378 T8805 T8801 arg1Of is,that
R7379 T8805 T8806 arg1Of is,not
R7380 T8808 T8805 arg2Of result,is
R7381 T8808 T8807 arg1Of result,a
R7382 T8808 T8809 arg1Of result,of
R7383 T8743 T8741 arg1Of regulation,the
R7384 T8743 T8742 arg1Of regulation,normal
R7385 T8812 T8809 arg2Of expression,of
R7386 T8812 T8810 arg1Of expression,differential
R7387 T8812 T8811 arg1Of expression,TLR
R7388 T8812 T8813 arg1Of expression,on
R7389 T8743 T8744 arg1Of regulation,of
R7390 T8746 T8747 arg1Of homeostasis,and
R7391 T8815 T8813 arg2Of cells,on
R7392 T8815 T8814 arg1Of cells,target
R7393 T8816 T8817 arg1Of LPS,engages
R7394 T8817 T8819 modOf engages,to
R7395 T8817 T8826 arg1Of engages,whereas
R7396 T8818 T8817 arg2Of TLR4,engages
R7397 T8820 T8819 arg1Of activate,to
R7398 T8747 T8744 arg2Of and,of
R7399 T8821 T8822 arg1Of MYD88-dependent,and
R7400 T8747 T8745 arg1Of and,DC
R7401 T8823 T8822 arg2Of TRIF-dependent,and
R7402 T8824 T8820 arg2Of pathways,activate
R7403 T8824 T8821 arg1Of pathways,MYD88-dependent
R7404 T8824 T8823 arg1Of pathways,TRIF-dependent
R7405 T8826 T8825 arg1Of whereas,","
R7406 T8827 T8828 arg2Of TLR3,stimulated
R7407 T8827 T8835 arg1Of TLR3,triggers
R7408 T8748 T8747 arg2Of maturation,and
R7409 T8831 T8830 arg1Of I,poly
R7410 T8831 T8832 arg1Of I,:
R7411 T8751 T8749 arg1Of basis,The
R7412 T8833 T8828 arg1Of C,stimulated
R7413 T8833 T8829 arg2Of C,by
R7414 T8833 T8831 arg1Of C,I
R7415 T8835 T8826 arg2Of triggers,whereas
R7416 T8835 T8834 arg1Of triggers,only
R7417 T8837 T8835 arg2Of signaling,triggers
R7418 T8837 T8836 arg1Of signaling,TRIF-dependent
R7419 T8837 T8838 arg1Of signaling,[
R7420 T8751 T8750 arg1Of basis,molecular
R7421 T8751 T8752 arg2Of basis,by
R7422 T8751 T8753 arg1Of basis,which
R7423 T8751 T8763 arg1Of basis,remains
R7424 T8839 T8838 arg2Of 53,[
R7425 T8751 T8765 arg1Of basis,be
R7426 T8840 T8838 arg3Of ],[
R7427 T8751 T8766 arg2Of basis,determined
R7428 T8756 T8754 arg1Of mutation,the
R7429 T8844 T8841 arg1Of activation,The
R7430 T8844 T8842 arg1Of activation,defective
R7431 T8844 T8843 arg1Of activation,NF-κB
R7432 T8844 T8845 arg1Of activation,by
R7433 T8844 T8848 arg1Of activation,suggests
R7434 T8847 T8845 arg2Of stimuli,by
R7435 T8847 T8846 arg1Of stimuli,both
R7436 T8756 T8755 arg1Of mutation,Sharpin
R7437 T8852 T8850 arg1Of mutation,the
R7438 T8852 T8851 arg1Of mutation,Sharpin
R7439 T8852 T8853 arg1Of mutation,interferes
R7440 T8756 T8757 arg1Of mutation,causes
R7441 T8853 T8848 arg2Of interferes,suggests
R7442 T8853 T8849 arg1Of interferes,that
R7443 T8853 T8854 arg1Of interferes,with
R7444 T8757 T8752 arg1Of causes,by
R7445 T8857 T8858 arg1Of adaptors,or
R7446 T8760 T8757 arg2Of activation,causes
R7447 T8858 T8854 arg2Of or,with
R7448 T8858 T8855 arg1Of or,the
R7449 T8858 T8856 arg1Of or,protein
R7450 T8858 T8860 arg2Of or,shared
R7451 T8859 T8858 arg2Of kinases,or
R7452 T8864 T8860 arg1Of pathways,shared
R7453 T8864 T8861 arg2Of pathways,by
R7454 T8864 T8862 arg1Of pathways,both
R7455 T8864 T8863 arg1Of pathways,signaling
R7456 T8864 T8865 arg1Of pathways,","
R7457 T8864 T8867 arg1Of pathways,as
R7458 T8867 T8866 arg1Of as,such
R7459 T8868 T8869 arg1Of RIP1,and
R7460 T8760 T8758 arg2Of activation,reduced
R7461 T8869 T8867 arg2Of and,as
R7462 T8760 T8759 arg1Of activation,NF-κB
R7463 T8760 T8761 arg1Of activation,in
R7464 T8762 T8761 arg2Of BMDC,in
R7465 T8869 T8871 arg1Of and,[
R7466 T8766 T8763 arg2Of determined,remains
R7467 T8870 T8869 arg2Of TRAF6,and
R7468 T8872 T8871 arg2Of 18,[
R7469 T8873 T8871 arg3Of ],[
R7470 T8766 T8764 arg1Of determined,to
R7471 T8875 T8874 arg1Of studies,Recent
R7472 T8875 T8876 arg1Of studies,demonstrated
R7473 T8766 T8765 arg2Of determined,be
R7474 T8767 T8768 arg1Of LPS,and
R7475 T8878 T8879 arg1Of SHARPIN,interacts
R7476 T8879 T8876 arg2Of interacts,demonstrated
R7477 T8879 T8877 arg1Of interacts,that
R7478 T8879 T8880 arg1Of interacts,with
R7479 T8881 T8880 arg2Of HOIP,with
R7480 T8881 T8882 modOf HOIP,to
R7481 T8768 T8771 arg1Of and,:
R7482 T8883 T8882 arg1Of form,to
R7483 T8884 T8883 arg2Of LUBAC,form
R7484 T8884 T8885 arg1Of LUBAC,that
R7485 T8884 T8886 arg1Of LUBAC,exerts
R7486 T8770 T8768 arg2Of I,and
R7487 T8889 T8886 arg2Of activity,exerts
R7488 T8889 T8887 arg1Of activity,its
R7489 T8889 T8888 arg1Of activity,linear-ubiquitin-chain-ligase
R7490 T8889 T8890 arg1Of activity,on
R7491 T8770 T8769 arg1Of I,poly
R7492 T8772 T8767 arg1Of C,LPS
R7493 T8894 T8891 arg1Of RIP1,NF-κB
R7494 T8894 T8892 arg1Of RIP1,signaling
R7495 T8894 T8893 arg1Of RIP1,players
R7496 T8894 T8895 arg1Of RIP1,and
R7497 T8895 T8890 arg2Of and,on
R7498 T8895 T8900 arg1Of and,","
R7499 T8896 T8895 arg2Of NEMO,and
R7500 T8896 T8897 arg1Of NEMO,[
R7501 T8772 T8770 arg1Of C,I
R7502 T8898 T8897 arg2Of 7,[
R7503 T8899 T8897 arg3Of ],[
R7504 T8772 T8773 arg2Of C,used
R7505 T8772 T8775 arg1Of C,are
R7506 T8773 T8774 arg1Of used,here
R7507 T8777 T8775 arg2Of ligands,are
R7508 T8903 T8900 arg2Of step,","
R7509 T8903 T8901 arg1Of step,an
R7510 T8903 T8902 arg1Of step,essential
R7511 T8903 T8904 arg1Of step,for
R7512 T8777 T8776 arg1Of ligands,well-defined
R7513 T8777 T8778 arg1Of ligands,that
R7514 T8777 T8780 arg1Of ligands,engage
R7515 T8780 T8779 arg1Of engage,specifically
R7516 T8908 T8904 arg2Of activation,for
R7517 T8908 T8905 arg1Of activation,intact
R7518 T8908 T8906 arg1Of activation,TNFα-stimulated
R7519 T8908 T8907 arg1Of activation,NF-κB
R7520 T8912 T8909 arg1Of mutation,The
R7521 T8912 T8910 arg1Of mutation,Sharpin
R7522 T8912 T8911 arg1Of mutation,null
R7523 T8912 T8913 arg1Of mutation,disrupts
R7524 T8780 T8784 arg1Of engage,","
R7525 T8912 T8918 arg1Of mutation,abrogates
R7526 T8780 T8785 arg1Of engage,respectively
R7527 T8913 T8917 arg1Of disrupts,and
R7528 T8781 T8782 arg1Of TLR4,and
R7529 T8782 T8780 arg2Of and,engage
R7530 T8916 T8913 arg2Of process,disrupts
R7531 T8916 T8914 arg1Of process,the
R7532 T8916 T8915 arg1Of process,ubiquitylation
R7533 T8918 T8917 arg2Of abrogates,and
R7534 T8783 T8782 arg2Of TLR3,and
R7535 T8788 T8786 arg1Of profile,The
R7536 T8923 T8918 arg2Of pathway,abrogates
R7537 T8923 T8919 arg1Of pathway,the
R7538 T8923 T8920 arg1Of pathway,TNFα-induced
R7539 T8923 T8921 arg1Of pathway,NF-κB
R7540 T8923 T8922 arg1Of pathway,signaling
R7541 T8925 T8926 arg1Of TNFR,and
R7542 T8926 T8929 arg1Of and,share
R7543 T8927 T8926 arg2Of TLR,and
R7544 T8929 T8924 arg2Of share,Since
R7545 T8929 T8928 arg1Of share,partially
R7546 T8788 T8787 arg1Of profile,expression
R7547 T8788 T8789 arg1Of profile,of
R7548 T8788 T8793 arg1Of profile,is
R7549 T8933 T8929 arg2Of cascades,share
R7550 T8933 T8930 arg1Of cascades,their
R7551 T8933 T8931 arg1Of cascades,downstream
R7552 T8933 T8932 arg1Of cascades,signaling
R7553 T8788 T8794 arg1Of profile,similar
R7554 T8938 T8935 arg1Of regulation,a
R7555 T8938 T8936 arg1Of regulation,similar
R7556 T8938 T8937 arg1Of regulation,ubiquitin-mediated
R7557 T8938 T8939 arg1Of regulation,may
R7558 T8938 T8940 arg1Of regulation,hold
R7559 T8938 T8941 arg1Of regulation,true
R7560 T8940 T8924 arg1Of hold,Since
R7561 T8940 T8934 arg1Of hold,","
R7562 T8940 T8939 arg2Of hold,may
R7563 T8792 T8789 arg2Of complexes,of
R7564 T8941 T8940 arg2Of true,hold
R7565 T8941 T8942 arg1Of true,for
R7566 T8943 T8942 arg2Of SHARPIN,for
R7567 T8943 T8944 arg1Of SHARPIN,in
R7568 T8943 T8949 arg1Of SHARPIN,:
R7569 T8945 T8946 arg1Of LPS-,and
R7570 T8946 T8944 arg2Of and,in
R7571 T8947 T8946 arg2Of poly,and
R7572 T8947 T8948 arg1Of poly,I
R7573 T8792 T8790 arg1Of complexes,surface
R7574 T8792 T8791 arg1Of complexes,TLR4
R7575 T8793 T8800 modOf is,suggesting
R7576 T8952 T8949 arg2Of activation,:
R7577 T8952 T8950 arg1Of activation,C-induced
R7578 T8952 T8951 arg1Of activation,NF-κB
R7579 T8794 T8793 arg2Of similar,is
R7580 T8954 T8953 arg2Of summary,In
R7581 T8794 T8795 arg1Of similar,between
R7582 T8796 T8797 arg1Of WT,and
R7583 T8958 T8956 arg1Of study,the
R7584 T8958 T8957 arg1Of study,present
R7585 T8958 T8959 arg1Of study,identified
R7586 T8959 T8953 arg1Of identified,In
R7587 T8959 T8955 arg1Of identified,","
R7588 T8962 T8959 arg2Of role,identified
R7589 T8962 T8960 arg1Of role,an
R7590 T8962 T8961 arg1Of role,indispensible
R7591 T8962 T8963 arg1Of role,of
R7592 T8962 T8965 arg1Of role,in
R7593 T8964 T8963 arg2Of SHARPIN,of
R7594 T8797 T8795 arg2Of and,between
R7595 T8799 T8797 arg2Of BMDC,and
R7596 T8967 T8965 arg2Of production,in
R7597 T8967 T8966 arg1Of production,the
R7598 T8967 T8968 arg1Of production,of
R7599 T8970 T8969 arg1Of mediators,pro-inflammatory
R7600 T8970 T8971 arg1Of mediators,and
R7601 T8971 T8968 arg2Of and,of
R7602 T8974 T8971 arg2Of signaling,and
R7603 T8974 T8972 arg1Of signaling,TLR-induced
R7604 T8974 T8973 arg1Of signaling,NF-κB
R7605 T8799 T8798 arg1Of BMDC,cpdm
R7606 T8998 T8994 arg2Of differentiation,between
R7607 T8977 T8976 arg1Of Th1-stimulating,impaired
R7608 T8978 T8975 arg1Of ability,The
R7609 T8978 T8977 arg1Of ability,Th1-stimulating
R7610 T8978 T8979 arg1Of ability,of
R7611 T8978 T8982 arg1Of ability,may
R7612 T8978 T8983 arg1Of ability,account
R7613 T8981 T8979 arg2Of DC,of
R7614 T8981 T8980 arg1Of DC,Sharpin-deficient
R7615 T8983 T8982 arg2Of account,may
R7616 T8983 T8984 arg1Of account,for
R7617 T8998 T8995 arg1Of differentiation,Th1
R7618 T8998 T8997 arg1Of differentiation,Th2
R7619 T8988 T8984 arg2Of phenotype,for
R7620 T8988 T8985 arg1Of phenotype,the
R7621 T8988 T8986 arg1Of phenotype,Th2-dominant
R7622 T8988 T8987 arg1Of phenotype,inflammatory
R7623 T8988 T8989 arg1Of phenotype,of
R7624 T9000 T8999 arg2Of critical,is
R7625 T8991 T8989 arg2Of mice,of
R7626 T8991 T8990 arg1Of mice,cpdm
R7627 T9000 T9001 arg1Of critical,for
R7628 T9003 T9001 arg2Of homeostasis,for
R7629 T8993 T8992 arg1Of balance,The
R7630 T8993 T8994 arg1Of balance,between
R7631 T8993 T8999 arg1Of balance,is
R7632 T8993 T9000 arg1Of balance,critical
R7633 T8995 T8996 arg1Of Th1,and
R7634 T8997 T8996 arg2Of Th2,and
R7635 T9003 T9002 arg1Of homeostasis,immune
R7636 T9006 T9004 arg1Of understanding,A
R7637 T9006 T9005 arg1Of understanding,better
R7638 T9006 T9007 arg1Of understanding,of
R7639 T9006 T9013 arg1Of understanding,will
R7640 T9006 T9014 arg1Of understanding,help
R7642 T9006 T9015 arg1Of understanding,design
R7643 T9008 T9007 arg2Of how,of
R7646 T9010 T9009 arg1Of balance,such
R7647 T9010 T9011 arg1Of balance,is
R7648 T9010 T9012 arg2Of balance,maintained
R7649 T9012 T9008 arg1Of maintained,how
R7650 T9012 T9011 arg2Of maintained,is
R7651 T9015 T9013 arg2Of design,will
R7652 T9015 T9014 arg2Of design,help
R7653 T9017 T9015 arg2Of treatment,design
R7654 T9017 T9016 arg1Of treatment,cytokine
R7655 T9017 T9018 arg1Of treatment,for
R7656 T9017 T9030 arg1Of treatment,","
R7657 T9017 T9032 arg1Of treatment,as
R7658 T9020 T9018 arg2Of diseases,for
R7659 T9020 T9019 arg1Of diseases,human
R7660 T9020 T9021 arg1Of diseases,with
R7661 T9024 T9021 arg2Of secretion,with
R7662 T9024 T9022 arg1Of secretion,Th2-biased
R7663 T9024 T9023 arg1Of secretion,cytokine
R7664 T9024 T9025 arg1Of secretion,similar
R7665 T9025 T9026 arg1Of similar,to
R7666 T9029 T9026 arg2Of cpdm,to
R7667 T9029 T9027 arg1Of cpdm,the
R7668 T9029 T9028 arg1Of cpdm,mouse
R7669 T9032 T9031 arg1Of as,such
R7670 T9033 T9034 arg1Of allergies,and
R7671 T9034 T9032 arg2Of and,as
R7672 T9036 T9034 arg2Of syndromes,and
R7673 T9036 T9035 arg1Of syndromes,hypereosinophilic
R7864 T9603 T9602 arg1Of Statement,Ethics
R7865 T9606 T9604 arg1Of work,All
R7866 T9606 T9605 arg1Of work,mouse
R7867 T9606 T9607 arg1Of work,was
R7868 T9606 T9608 arg2Of work,carried
R7869 T9608 T9607 arg2Of carried,was
R7870 T9608 T9609 arg1Of carried,out
R7871 T9608 T9610 arg1Of carried,in
R7873 T9612 T9610 arg2Of accordance,in
R8035 T9789 T9787 arg1Of colonies,Specific-pathogen
R8036 T9789 T9788 arg1Of colonies,free
R8037 T9789 T9790 arg1Of colonies,of
R8038 T9789 T9792 arg1Of colonies,(
R8039 T9789 T9797 arg1Of colonies,and
R8040 T9791 T9790 arg2Of C57BL/KaLawRij-Sharpincpdm/RijSunJ,of
R8041 T9793 T9792 arg2Of JR,(
R8042 T9793 T9795 arg1Of JR,7599
R8043 T9795 T9794 arg1Of 7599,#
R8044 T9796 T9792 arg3Of ),(
R8045 T9797 T9800 arg1Of and,were
R8046 T9797 T9801 arg2Of and,obtained
R8047 T9797 T9813 arg1Of and,were
R8048 T9797 T9814 arg2Of and,maintained
R8049 T9799 T9797 arg2Of mice,and
R8050 T9799 T9798 arg1Of mice,WT
R8051 T9801 T9800 arg2Of obtained,were
R8052 T9801 T9802 arg1Of obtained,from
R8053 T9801 T9812 arg1Of obtained,and
R8054 T9805 T9802 arg2Of Laboratory,from
R8055 T9805 T9803 arg1Of Laboratory,The
R8056 T9805 T9804 arg1Of Laboratory,Jackson
R8057 T9805 T9806 arg1Of Laboratory,(
R8058 T9808 T9806 arg2Of Harbor,(
R8059 T9808 T9807 arg1Of Harbor,Bar
R8060 T9808 T9809 arg1Of Harbor,","
R8061 T9810 T9809 arg2Of ME,","
R8062 T9811 T9806 arg3Of ),(
R8063 T9814 T9812 arg2Of maintained,and
R8064 T9814 T9813 arg2Of maintained,were
R8065 T9814 T9815 arg1Of maintained,in
R8066 T9818 T9815 arg2Of facility,in
R8067 T9818 T9816 arg1Of facility,a
R8068 T9818 T9817 arg1Of facility,barrier
R8069 T9821 T9819 arg1Of controls,Normal
R8070 T9821 T9820 arg1Of controls,littermate
R8071 T9821 T9822 arg1Of controls,were
R8072 T9822 T9825 arg1Of were,or
R8073 T9824 T9822 arg2Of +/+,were
R8074 T9824 T9823 arg1Of +/+,either
R8075 T9829 T9826 arg1Of animals,+/Sharpincpdm.
R8076 T9829 T9827 arg1Of animals,These
R8077 T9829 T9828 arg1Of animals,control
R8078 T9829 T9830 arg1Of animals,were
R8079 T9829 T9832 arg1Of animals,indistinguishable
R8080 T9829 T9834 arg1Of animals,are
R8081 T9829 T9835 arg2Of animals,referred
R8082 T9830 T9833 arg1Of were,and
R8083 T9832 T9830 arg2Of indistinguishable,were
R8084 T9832 T9831 arg1Of indistinguishable,phenotypically
R8085 T9833 T9825 arg2Of and,or
R8086 T9835 T9833 arg2Of referred,and
R8087 T9835 T9834 arg2Of referred,are
R8088 T9835 T9836 arg1Of referred,to
R8089 T9835 T9837 arg1Of referred,as
R8090 T9838 T9837 arg2Of WT,as
R8091 T9839 T9840 arg1Of Sex-matched,WT
R8092 T9839 T9841 arg1Of Sex-matched,and
R8093 T9842 T9841 arg2Of mutant,and
R8094 T9843 T9839 arg1Of mice,Sex-matched
R8095 T9843 T9842 arg1Of mice,mutant
R8096 T9843 T9844 arg1Of mice,were
R8097 T9843 T9845 arg2Of mice,used
R8098 T9845 T9844 arg2Of used,were
R8099 T9845 T9846 arg1Of used,at
R8100 T9848 T9846 arg2Of weeks,at
R8101 T9848 T9847 arg1Of weeks,6–10
R8102 T9848 T9849 arg1Of weeks,of
R8103 T9850 T9849 arg2Of age,of
R8104 T9853 T9851 arg2Of experiments,For
R8105 T9853 T9852 arg1Of experiments,some
R8106 T9856 T9855 arg1Of mice,cpdm
R8107 T9856 T9857 arg1Of mice,were
R8108 T9856 T9858 arg2Of mice,crossed
R8109 T9858 T9851 arg1Of crossed,For
R8110 T9858 T9854 arg1Of crossed,","
R8111 T9858 T9857 arg2Of crossed,were
R8112 T9858 T9859 arg1Of crossed,with
R8113 T9858 T9862 arg1Of crossed,with
R8114 T9861 T9859 arg2Of mice,with
R8115 T9861 T9860 arg1Of mice,transgenic
R8116 T9866 T9862 arg2Of chromosome,with
R8117 T9866 T9863 arg1Of chromosome,a
R8118 T9866 T9864 arg1Of chromosome,bacterial
R8119 T9866 T9865 arg1Of chromosome,artificial
R8120 T9866 T9867 arg1Of chromosome,(
R8121 T9866 T9870 arg1Of chromosome,containing
R8122 T9868 T9867 arg2Of BAC,(
R8123 T9869 T9867 arg3Of ),(
R8124 T9873 T9870 arg2Of gene,containing
R8125 T9873 T9871 arg1Of gene,the
R8126 T9873 T9872 arg1Of gene,Sharpin
R8127 T9873 T9874 arg1Of gene,(
R8128 T9877 T9876 arg2Of RP24-173I23,(
R8129 T9878 T9876 arg3Of ),(
R8130 T9879 T9874 arg2Of 1Sun/Sun,(
R8131 T9879 T9875 arg1Of 1Sun/Sun,FVB/NJ-Tg
R8132 T9879 T9876 arg1Of 1Sun/Sun,(
R8133 T9879 T9880 arg1Of 1Sun/Sun,","
R8134 T9881 T9880 arg2Of JR,","
R8135 T9881 T9883 arg1Of JR,8279
R8136 T9883 T9882 arg1Of 8279,#
R8137 T9884 T9874 arg3Of ),(
R8138 T9886 T9885 arg1Of mice,These
R8139 T9886 T9887 arg1Of mice,were
R8140 T9886 T9888 arg2Of mice,backcrossed
R8141 T9888 T9887 arg2Of backcrossed,were
R8142 T9888 T9889 arg1Of backcrossed,onto
R8143 T9888 T9893 arg1Of backcrossed,and
R8144 T9892 T9889 arg2Of background,onto
R8145 T9892 T9890 arg1Of background,the
R8146 T9892 T9891 arg1Of background,C57BL/KaLawRij-Sharpincpdm/RijSunJ
R8147 T9895 T9894 arg1Of mice,N4
R8148 T9895 T9896 arg1Of mice,were
R8149 T9895 T9897 arg2Of mice,used
R8150 T9897 T9893 arg2Of used,and
R8151 T9897 T9896 arg2Of used,were
R8152 T9897 T9898 arg1Of used,in
R8153 T9900 T9898 arg2Of experiments,in
R8154 T9900 T9899 arg1Of experiments,the
R8155 T9900 T9901 arg2Of experiments,reported
R8156 T9901 T9902 arg1Of reported,here
R8157 T9905 T9903 arg1Of work,All
R8158 T9905 T9904 arg1Of work,mouse
R8159 T9905 T9906 arg1Of work,was
R8160 T9905 T9907 arg2Of work,carried
R8161 T9907 T9906 arg2Of carried,was
R8162 T9907 T9908 arg1Of carried,out
R8163 T9907 T9909 arg1Of carried,in
R8164 T9911 T9909 arg2Of accordance,in
R8165 T9911 T9910 arg1Of accordance,strict
R8166 T9911 T9912 arg1Of accordance,with
R8167 T9915 T9912 arg2Of protocols,with
R8168 T9915 T9913 arg1Of protocols,the
R8169 T9915 T9914 arg2Of protocols,approved
R8170 T9920 T9918 arg1Of Care,Institutional
R8171 T9920 T9919 arg1Of Care,Animal
R8172 T9920 T9921 arg1Of Care,and
R8173 T9921 T9907 arg1Of and,carried
R8174 T9921 T9916 arg2Of and,by
R8175 T9921 T9917 arg1Of and,the
R8176 T9923 T9921 arg2Of Committee,and
R8177 T9923 T9922 arg1Of Committee,Use
R8350 T10135 T10136 arg1Of Constructs,and
R8351 T10137 T10136 arg2Of transfection,and
R8352 T10140 T10138 arg1Of DNA,The
R8353 T10140 T10139 arg1Of DNA,complementary
R8354 T10140 T10141 arg1Of DNA,(
R8355 T10140 T10144 arg1Of DNA,of
R8356 T10140 T10149 arg1Of DNA,was
R8357 T10140 T10150 arg2Of DNA,cloned
R8358 T10140 T10152 arg2Of DNA,amplified
R8359 T10142 T10141 arg2Of cDNA,(
R8360 T10143 T10141 arg3Of ),(
R8361 T10148 T10144 arg2Of gene,of
R8362 T10148 T10145 arg1Of gene,the
R8363 T10148 T10146 arg1Of gene,mouse
R8364 T10148 T10147 arg1Of gene,Sharpin
R8365 T10150 T10151 arg1Of cloned,and
R8366 T10151 T10149 arg2Of and,was
R8367 T10151 T10153 arg1Of and,from
R8368 T10152 T10151 arg2Of amplified,and
R8369 T10156 T10153 arg2Of extracts,from
R8370 T10156 T10154 arg1Of extracts,RAW264.7
R8371 T10156 T10155 arg1Of extracts,RNA
R8372 T10159 T10157 arg1Of sequences,The
R8373 T10159 T10158 arg1Of sequences,primer
R8374 T10159 T10160 arg1Of sequences,were
R8375 T10160 T10161 arg1Of were,forward
R8376 T10160 T10162 arg1Of were,","
R8377 T10160 T10173 arg1Of were,;
R8378 T10172 T10160 arg2Of GGT,were
R8379 T10172 T10163 arg1Of GGT,5′-CC
R8380 T10172 T10164 arg1Of GGT,ATG
R8381 T10172 T10165 arg1Of GGT,GCG
R8382 T10172 T10166 arg1Of GGT,ATG
R8383 T10172 T10167 arg1Of GGT,TCG
R8384 T10172 T10168 arg1Of GGT,CCG
R8385 T10172 T10169 arg1Of GGT,CCC
R8386 T10172 T10170 arg1Of GGT,GCC
R8387 T10172 T10171 arg1Of GGT,GGC
R8388 T10174 T10175 arg1Of reverse,","
R8389 T10179 T10176 arg1Of CTA,5′-
R8390 T10179 T10177 arg1Of CTA,AAG
R8391 T10179 T10178 arg1Of CTA,CTT
R8392 T10184 T10180 arg1Of AGC,GGT
R8393 T10184 T10181 arg1Of AGC,GGA
R8394 T10184 T10182 arg1Of AGC,AGC
R8395 T10184 T10183 arg1Of AGC,TGC
R8396 T10186 T10175 arg2Of A.,","
R8397 T10186 T10179 arg1Of A.,CTA
R8398 T10186 T10184 arg1Of A.,AGC
R8399 T10186 T10185 arg1Of A.,AAG
R8400 T10189 T10187 arg1Of cDNA,The
R8401 T10189 T10188 arg1Of cDNA,Sharpin
R8402 T10189 T10190 arg1Of cDNA,was
R8403 T10189 T10191 arg2Of cDNA,cloned
R8404 T10191 T10173 arg2Of cloned,;
R8405 T10191 T10174 arg1Of cloned,reverse
R8406 T10191 T10190 arg2Of cloned,was
R8407 T10191 T10192 arg1Of cloned,into
R8408 T10195 T10192 arg2Of pFLAG-CMV-2,into
R8409 T10195 T10193 arg1Of pFLAG-CMV-2,expression
R8410 T10195 T10194 arg1Of pFLAG-CMV-2,vector
R8411 T10197 T10196 arg1Of fibroblasts,Murine
R8412 T10197 T10200 arg2Of fibroblasts,were
R8413 T10197 T10201 arg1Of fibroblasts,were
R8414 T10197 T10202 arg2Of fibroblasts,used
R8415 T10199 T10200 arg1Of macrophages,were
R8416 T10200 T10198 arg1Of were,and
R8417 T10202 T10201 arg2Of used,were
R8418 T10202 T10203 modOf used,to
R8419 T10204 T10203 arg1Of express,to
R8420 T10207 T10204 arg2Of protein,express
R8421 T10207 T10205 arg1Of protein,recombinant
R8422 T10207 T10206 arg1Of protein,SHARPIN
R8423 T10208 T10209 arg1Of Cells,(
R8424 T10208 T10212 arg1Of Cells,were
R8425 T10208 T10213 arg2Of Cells,seeded
R8426 T10210 T10209 arg2Of 2×104,(
R8427 T10211 T10209 arg3Of ),(
R8428 T10213 T10212 arg2Of seeded,were
R8429 T10213 T10214 arg1Of seeded,in
R8430 T10213 T10219 arg1Of seeded,day
R8431 T10213 T10220 arg1Of seeded,before
R8432 T10217 T10214 arg2Of plates,in
R8433 T10217 T10215 arg1Of plates,96-well
R8434 T10217 T10216 arg2Of plates,treated
R8435 T10219 T10218 arg1Of day,the
R8436 T10221 T10220 arg2Of transfection,before
R8437 T10224 T10222 arg2Of incubation,After
R8438 T10224 T10223 arg1Of incubation,overnight
R8439 T10229 T10226 arg1Of plasmids,200
R8440 T10229 T10227 arg1Of plasmids,ng
R8441 T10229 T10228 arg1Of plasmids,pFLAG-SHARPIN
R8442 T10229 T10230 arg1Of plasmids,were
R8443 T10229 T10231 arg2Of plasmids,transfected
R8444 T10231 T10222 arg1Of transfected,After
R8445 T10231 T10225 arg1Of transfected,","
R8446 T10231 T10230 arg2Of transfected,were
R8447 T10231 T10232 arg1Of transfected,with
R8448 T10235 T10232 arg2Of Lipofectamine,with
R8449 T10235 T10233 arg1Of Lipofectamine,0.5
R8450 T10235 T10234 arg1Of Lipofectamine,ul
R8451 T10235 T10236 arg1Of Lipofectamine,2000
R8452 T10238 T10237 arg1Of hours,24
R8453 T10239 T10238 arg1Of later,hours
R8454 T10241 T10242 arg1Of cells,were
R8455 T10241 T10243 arg2Of cells,incubated
R8456 T10243 T10239 arg1Of incubated,later
R8457 T10243 T10240 arg1Of incubated,","
R8458 T10243 T10242 arg2Of incubated,were
R8459 T10243 T10244 arg1Of incubated,with
R8460 T10247 T10244 arg2Of medium,with
R8461 T10247 T10245 arg1Of medium,fresh
R8462 T10247 T10246 arg1Of medium,culture
R8463 T10251 T10248 arg2Of hours,After
R8464 T10251 T10249 arg1Of hours,another
R8465 T10251 T10250 arg1Of hours,24
R8466 T10253 T10254 arg1Of cells,were
R8467 T10253 T10255 arg2Of cells,lysed
R8468 T10253 T10257 arg1Of cells,confirm
R8469 T10255 T10248 arg1Of lysed,After
R8470 T10255 T10252 arg1Of lysed,","
R8471 T10255 T10254 arg2Of lysed,were
R8472 T10257 T10255 arg3Of confirm,lysed
R8473 T10257 T10256 arg1Of confirm,to
R8474 T10260 T10257 arg2Of expression,confirm
R8475 T10260 T10258 arg1Of expression,the
R8476 T10260 T10259 arg1Of expression,FLAG-SHARPIN
R8477 T10260 T10261 arg1Of expression,by
R8478 T10262 T10261 arg2Of immunoblots,by
R8479 T10262 T10263 arg1Of immunoblots,with
R8480 T10264 T10263 arg2Of anti-FLAG,with
R8481 T10265 T10266 arg1Of Cells,with
R8482 T10265 T10270 arg2Of Cells,transfected
R8483 T10265 T10275 arg1Of Cells,were
R8484 T10265 T10276 arg2Of Cells,used
R8485 T10266 T10269 arg1Of with,and
R8486 T10268 T10266 arg2Of transfection,with
R8487 T10268 T10267 arg1Of transfection,no
R8488 T10270 T10269 arg2Of transfected,and
R8489 T10270 T10271 arg1Of transfected,with
R8490 T10274 T10271 arg2Of pFLAG-CMV-2,with
R8491 T10274 T10272 arg1Of pFLAG-CMV-2,empty
R8492 T10274 T10273 arg1Of pFLAG-CMV-2,vector
R8493 T10276 T10275 arg2Of used,were
R8494 T10276 T10277 arg1Of used,as
R8495 T10279 T10277 arg2Of control,as
R8496 T10279 T10278 arg1Of control,negative
R8497 T10279 T10280 arg1Of control,in
R8498 T10283 T10280 arg2Of experiments,in
R8499 T10283 T10281 arg1Of experiments,all
R8500 T10283 T10282 arg1Of experiments,transfection
R8552 T10381 T10382 arg1Of Generation,of
R8553 T10383 T10382 arg2Of BMDC,of
R8554 T10384 T10385 arg1Of BMDC,were
R8555 T10384 T10386 arg2Of BMDC,developed
R8556 T10386 T10385 arg2Of developed,were
R8557 T10386 T10387 arg1Of developed,as
R8558 T10386 T10393 arg1Of developed,","
R8559 T10386 T10394 arg1Of developed,[
R8560 T10389 T10388 arg1Of reported,previously
R8561 T10391 T10387 arg2Of 54,as
R8562 T10391 T10389 arg1Of 54,reported
R8563 T10391 T10390 arg2Of 54,[
R8564 T10392 T10390 arg3Of ],[
R8565 T10395 T10394 arg2Of 55,[
R8566 T10396 T10394 arg3Of ],[
R8567 T10397 T10398 arg1Of Cells,were
R8568 T10397 T10399 arg2Of Cells,collected
R8569 T10399 T10398 arg2Of collected,were
R8570 T10399 T10400 arg1Of collected,after
R8571 T10402 T10400 arg2Of days,after
R8572 T10402 T10401 arg1Of days,10–12
R8573 T10402 T10403 arg1Of days,of
R8574 T10404 T10403 arg2Of culture,of
R8575 T10407 T10405 arg1Of yield,The
R8576 T10407 T10406 arg1Of yield,cell
R8577 T10407 T10408 arg1Of yield,was
R8578 T10410 T10408 arg2Of cells/mouse,was
R8579 T10410 T10409 arg1Of cells/mouse,2–3×107
R8580 T10410 T10411 arg1Of cells/mouse,with
R8581 T10412 T10413 arg1Of 80–95,%
R8582 T10414 T10411 arg2Of BMDC,with
R8583 T10414 T10412 arg1Of BMDC,80–95
R8828 T10676 T10677 arg1Of Phenotype,of
R8829 T10678 T10679 arg1Of BMDC,and
R8830 T10680 T10679 arg2Of splenic,and
R8831 T10682 T10677 arg2Of subsets,of
R8832 T10682 T10678 arg1Of subsets,BMDC
R8833 T10682 T10680 arg1Of subsets,splenic
R8834 T10682 T10681 arg1Of subsets,DC
R8835 T10684 T10683 arg1Of phenotype,The
R8836 T10684 T10685 arg1Of phenotype,of
R8837 T10684 T10687 arg1Of phenotype,was
R8838 T10684 T10688 arg2Of phenotype,determined
R8839 T10686 T10685 arg2Of BMDC,of
R8840 T10688 T10687 arg2Of determined,was
R8841 T10688 T10689 arg1Of determined,before
R8842 T10688 T10691 arg1Of determined,after
R8843 T10688 T10695 arg1Of determined,in
R8844 T10689 T10690 arg1Of before,and
R8845 T10691 T10690 arg2Of after,and
R8846 T10692 T10693 arg1Of 24,hour
R8847 T10694 T10689 arg2Of culture,before
R8848 T10694 T10691 arg2Of culture,after
R8849 T10694 T10692 arg1Of culture,24
R8850 T10695 T10696 arg1Of in,the
R8851 T10695 T10698 arg1Of in,of
R8852 T10697 T10695 arg2Of presence,in
R8853 T10699 T10700 arg1Of 100,ng/mL
R8854 T10701 T10695 arg3Of LPS,in
R8855 T10701 T10699 arg1Of LPS,100
R8856 T10703 T10702 arg1Of cells,The
R8857 T10703 T10704 arg1Of cells,were
R8858 T10703 T10705 arg2Of cells,incubated
R8859 T10703 T10730 arg2Of cells,incubated
R8860 T10705 T10706 arg1Of incubated,in
R8861 T10705 T10708 arg1Of incubated,with
R8862 T10705 T10729 arg1Of incubated,and
R8863 T10707 T10706 arg2Of PBS,in
R8864 T10709 T10710 arg1Of 0.1,%
R8865 T10711 T10709 arg1Of NaN3,0.1
R8866 T10711 T10712 arg1Of NaN3,","
R8867 T10712 T10716 arg1Of ",",and
R8868 T10713 T10714 arg1Of 1,%
R8869 T10715 T10712 arg2Of BSA,","
R8870 T10715 T10713 arg1Of BSA,1
R8871 T10716 T10708 arg2Of and,with
R8872 T10716 T10727 arg1Of and,","
R8873 T10716 T10728 arg2Of and,washed
R8874 T10717 T10718 arg1Of 10,%
R8875 T10721 T10716 arg2Of serum,and
R8876 T10721 T10717 arg1Of serum,10
R8877 T10721 T10719 arg1Of serum,normal
R8878 T10721 T10720 arg1Of serum,rabbit
R8879 T10721 T10722 arg1Of serum,for
R8880 T10721 T10725 arg1Of serum,on
R8881 T10724 T10722 arg2Of minutes,for
R8882 T10724 T10723 arg1Of minutes,20
R8883 T10726 T10725 arg2Of ice,on
R8884 T10729 T10704 arg2Of and,were
R8885 T10730 T10729 arg2Of incubated,and
R8886 T10730 T10731 arg1Of incubated,for
R8887 T10730 T10734 arg1Of incubated,on
R8888 T10733 T10731 arg2Of minutes,for
R8889 T10733 T10732 arg1Of minutes,30
R8890 T10735 T10734 arg2Of ice,on
R8891 T10735 T10736 arg1Of ice,with
R8892 T10739 T10736 arg2Of anti-CD11c,with
R8893 T10739 T10737 arg1Of anti-CD11c,Alex
R8894 T10739 T10738 arg1Of anti-CD11c,Fluor-labeled
R8895 T10739 T10740 arg1Of anti-CD11c,(
R8896 T10739 T10745 arg1Of anti-CD11c,in
R8897 T10739 T10750 arg1Of anti-CD11c,(
R8898 T10739 T10756 arg1Of anti-CD11c,","
R8899 T10741 T10740 arg2Of MCD11C20,(
R8900 T10741 T10742 arg1Of MCD11C20,","
R8901 T10743 T10742 arg2Of CALTAG,","
R8902 T10744 T10740 arg3Of ),(
R8903 T10746 T10745 arg2Of combination,in
R8904 T10746 T10747 arg1Of combination,with
R8905 T10749 T10747 arg2Of anti-CD40,with
R8906 T10749 T10748 arg1Of anti-CD40,PE-
R8907 T10751 T10750 arg2Of 3/23,(
R8908 T10751 T10752 arg1Of 3/23,","
R8909 T10754 T10752 arg2Of Biosciences,","
R8910 T10754 T10753 arg1Of Biosciences,BD
R8911 T10755 T10750 arg3Of ),(
R8912 T10757 T10758 arg1Of PE-anti-CD80,(
R8913 T10757 T10763 arg1Of PE-anti-CD80,","
R8914 T10759 T10758 arg2Of 16-10A1,(
R8915 T10759 T10760 arg1Of 16-10A1,","
R8916 T10761 T10760 arg2Of eBioscience,","
R8917 T10762 T10758 arg3Of ),(
R8918 T10763 T10765 arg1Of ",",(
R8919 T10763 T10770 arg1Of ",",","
R8920 T10764 T10763 arg2Of PE-anti-CD86,","
R8921 T10766 T10765 arg2Of P03.1,(
R8922 T10766 T10767 arg1Of P03.1,","
R8923 T10768 T10767 arg2Of eBioscience,","
R8924 T10769 T10765 arg3Of ),(
R8925 T10770 T10756 arg2Of ",",","
R8926 T10771 T10772 arg1Of PE-anti-CD14,(
R8927 T10771 T10777 arg1Of PE-anti-CD14,and
R8928 T10773 T10772 arg2Of Sa2.8,(
R8929 T10773 T10774 arg1Of Sa2.8,","
R8930 T10775 T10774 arg2Of eBioscience,","
R8931 T10776 T10772 arg3Of ),(
R8932 T10777 T10770 arg2Of and,","
R8933 T10779 T10777 arg2Of anti-TLR4,and
R8934 T10779 T10778 arg2Of anti-TLR4,biotinylated
R8935 T10779 T10780 arg1Of anti-TLR4,(
R8936 T10781 T10780 arg2Of BioLegend,(
R8937 T10782 T10780 arg3Of ),(
R8938 T10784 T10783 arg1Of cells,The
R8939 T10784 T10785 arg2Of cells,incubated
R8940 T10784 T10789 arg1Of cells,were
R8941 T10784 T10790 arg2Of cells,washed
R8942 T10784 T10793 arg2Of cells,incubated
R8943 T10785 T10786 arg1Of incubated,with
R8944 T10788 T10786 arg2Of anti-TLR4/MD-2,with
R8945 T10788 T10787 arg2Of anti-TLR4/MD-2,biotinylated
R8946 T10790 T10791 arg1Of washed,twice
R8947 T10790 T10792 arg1Of washed,and
R8948 T10792 T10789 arg2Of and,were
R8949 T10793 T10792 arg2Of incubated,and
R8950 T10793 T10794 arg1Of incubated,for
R8951 T10796 T10794 arg2Of minutes,for
R8952 T10796 T10795 arg1Of minutes,30
R8953 T10796 T10797 arg1Of minutes,on
R8954 T10798 T10797 arg2Of ice,on
R8955 T10798 T10799 arg1Of ice,with
R8956 T10800 T10799 arg2Of avidin-PE,with
R8957 T10802 T10801 arg1Of isolate,To
R8958 T10804 T10802 arg2Of DC,isolate
R8959 T10804 T10803 arg1Of DC,splenic
R8960 T10806 T10802 arg1Of spleens,isolate
R8961 T10806 T10807 arg1Of spleens,were
R8962 T10806 T10809 arg1Of spleens,digested
R8963 T10806 T10811 arg1Of spleens,subject
R8964 T10807 T10801 modOf were,To
R8965 T10807 T10805 arg1Of were,","
R8966 T10809 T10808 arg1Of digested,collagenase
R8967 T10809 T10810 arg1Of digested,and
R8968 T10810 T10807 arg2Of and,were
R8969 T10811 T10810 arg2Of subject,and
R8970 T10811 T10812 arg1Of subject,to
R8971 T10815 T10812 arg2Of centrifugation,to
R8972 T10815 T10813 arg1Of centrifugation,Percoll
R8973 T10815 T10814 arg1Of centrifugation,gradient
R8974 T10817 T10816 arg1Of bands,The
R8975 T10817 T10818 arg1Of bands,at
R8976 T10817 T10823 arg1Of bands,were
R8977 T10817 T10824 arg2Of bands,collected
R8978 T10820 T10821 arg1Of 35–55,%
R8979 T10822 T10818 arg2Of interface,at
R8980 T10822 T10819 arg1Of interface,the
R8981 T10822 T10820 arg1Of interface,35–55
R8982 T10824 T10823 arg2Of collected,were
R8983 T10824 T10825 modOf collected,to
R8984 T10826 T10825 arg1Of stain,to
R8985 T10826 T10827 arg1Of stain,with
R8986 T10828 T10829 arg1Of PE-anti-CD11c,(
R8987 T10828 T10834 arg1Of PE-anti-CD11c,","
R8988 T10830 T10829 arg2Of HL3,(
R8989 T10830 T10831 arg1Of HL3,","
R8990 T10830 T10832 arg1Of HL3,BioLegend
R8991 T10833 T10829 arg3Of ),(
R8992 T10834 T10836 arg1Of ",",(
R8993 T10834 T10841 arg1Of ",",and
R8994 T10835 T10834 arg2Of APC-anti-PDCA-1,","
R8995 T10837 T10836 arg2Of 927,(
R8996 T10837 T10838 arg1Of 927,","
R8997 T10839 T10838 arg2Of BioLegend,","
R8998 T10840 T10836 arg3Of ),(
R8999 T10841 T10827 arg2Of and,with
R9000 T10842 T10841 arg2Of FITC-anti-CD8α,and
R9001 T10842 T10843 arg1Of FITC-anti-CD8α,(
R9002 T10844 T10843 arg2Of 53–6.7,(
R9003 T10844 T10845 arg1Of 53–6.7,","
R9004 T10847 T10845 arg2Of BioScience,","
R9005 T10847 T10846 arg1Of BioScience,BD
R9006 T10848 T10843 arg3Of ),(
R9007 T10850 T10849 arg1Of cells,The
R9008 T10850 T10851 arg1Of cells,were
R9009 T10850 T10852 arg2Of cells,washed
R9010 T10850 T10855 arg2Of cells,fixed
R9011 T10850 T10862 arg1Of cells,stored
R9012 T10852 T10853 arg1Of washed,twice
R9013 T10852 T10854 arg1Of washed,","
R9014 T10854 T10851 arg2Of ",",were
R9015 T10854 T10861 arg1Of ",",and
R9016 T10855 T10854 arg2Of fixed,","
R9017 T10855 T10856 arg1Of fixed,in
R9018 T10857 T10858 arg1Of 2,%
R9019 T10859 T10856 arg2Of paraformaldehyde,in
R9020 T10859 T10857 arg1Of paraformaldehyde,2
R9021 T10861 T10860 arg1Of and,","
R9022 T10862 T10861 arg2Of stored,and
R9023 T10862 T10863 arg1Of stored,at
R9024 T10862 T10865 arg1Of stored,until
R9025 T10864 T10863 arg2Of 4°C,at
R9026 T10866 T10865 arg2Of analysis,until
R9027 T10868 T10867 arg1Of cytometry,Flow
R9028 T10868 T10869 arg1Of cytometry,was
R9029 T10868 T10870 arg2Of cytometry,performed
R9030 T10870 T10869 arg2Of performed,was
R9031 T10870 T10871 arg1Of performed,in
R9032 T10875 T10874 arg2Of Coulter,(
R9033 T10876 T10874 arg3Of ),(
R9034 T10877 T10871 arg2Of instrument,in
R9035 T10877 T10872 arg1Of instrument,an
R9036 T10877 T10873 arg1Of instrument,Excel
R9037 T10877 T10874 arg1Of instrument,(
R9038 T10879 T10878 arg1Of cells,Dead
R9039 T10879 T10880 arg1Of cells,were
R9040 T10879 T10881 arg2Of cells,omitted
R9041 T10879 T10886 arg1Of cells,gating
R9042 T10881 T10880 arg2Of omitted,were
R9043 T10881 T10882 arg1Of omitted,from
R9044 T10881 T10885 arg1Of omitted,by
R9045 T10881 T10894 arg1Of omitted,and
R9046 T10884 T10882 arg2Of analysis,from
R9047 T10884 T10883 arg1Of analysis,the
R9048 T10886 T10885 arg2Of gating,by
R9049 T10886 T10887 arg1Of gating,on
R9050 T10888 T10889 arg1Of forward,and
R9051 T10889 T10891 arg1Of and,light
R9052 T10890 T10889 arg2Of 90°,and
R9053 T10892 T10887 arg2Of scatter,on
R9054 T10892 T10888 arg1Of scatter,forward
R9055 T10892 T10890 arg1Of scatter,90°
R9056 T10894 T10893 arg1Of and,","
R9057 T10896 T10895 arg1Of cells,"10,000"
R9058 T10896 T10897 arg1Of cells,were
R9059 T10896 T10898 arg2Of cells,analyzed
R9060 T10898 T10894 arg2Of analyzed,and
R9061 T10898 T10897 arg2Of analyzed,were
R9062 T10901 T10898 arg1Of software,analyzed
R9063 T10901 T10899 arg2Of software,by
R9064 T10901 T10900 arg1Of software,FlowJo
R9225 T11114 T11113 arg1Of vitro,in
R9226 T11115 T11111 arg1Of interaction,BMDC-T
R9227 T11115 T11112 arg1Of interaction,cell
R9228 T11115 T11114 arg1Of interaction,vitro
R9229 T11116 T11117 arg1Of WT,and
R9230 T11118 T11117 arg2Of cpdm,and
R9231 T11119 T11116 arg1Of BMDC,WT
R9232 T11119 T11118 arg1Of BMDC,cpdm
R9233 T11119 T11120 arg1Of BMDC,(
R9234 T11119 T11123 arg1Of BMDC,were
R9235 T11119 T11124 arg2Of BMDC,stimulated
R9236 T11121 T11120 arg2Of 5×104,(
R9237 T11122 T11120 arg3Of ),(
R9238 T11124 T11123 arg2Of stimulated,were
R9239 T11124 T11125 arg1Of stimulated,with
R9240 T11126 T11127 arg1Of medium,","
R9241 T11127 T11131 arg1Of ",",","
R9242 T11130 T11127 arg2Of LPS,","
R9243 T11130 T11128 arg1Of LPS,1
R9244 T11130 T11129 arg1Of LPS,µg/mL
R9245 T11131 T11134 arg1Of ",",and
R9246 T11133 T11131 arg2Of µg/mL,","
R9247 T11133 T11132 arg1Of µg/mL,25
R9248 T11134 T11125 arg2Of and,with
R9249 T11137 T11134 arg2Of Pam3CYS,and
R9250 T11137 T11135 arg1Of Pam3CYS,5
R9251 T11137 T11136 arg1Of Pam3CYS,µg/mL
R9252 T11139 T11138 arg1Of hours,24
R9253 T11140 T11139 arg1Of later,hours
R9254 T11142 T11143 arg1Of cells,were
R9255 T11142 T11144 arg2Of cells,collected
R9256 T11142 T11146 arg2Of cells,washed
R9257 T11144 T11145 arg1Of collected,and
R9258 T11145 T11140 arg1Of and,later
R9259 T11145 T11141 arg1Of and,","
R9260 T11145 T11143 arg2Of and,were
R9261 T11146 T11145 arg2Of washed,and
R9262 T11146 T11147 arg1Of washed,with
R9263 T11148 T11147 arg2Of PBS,with
R9264 T11153 T11149 arg1Of cells,Allogeneic
R9265 T11153 T11150 arg1Of cells,naïve
R9266 T11153 T11151 arg1Of cells,CD4+
R9267 T11153 T11152 arg1Of cells,T
R9268 T11153 T11154 arg1Of cells,were
R9269 T11153 T11155 arg2Of cells,isolated
R9270 T11153 T11169 arg1Of cells,were
R9271 T11153 T11171 arg2Of cells,added
R9272 T11153 T11175 arg2Of cells,co-cultured
R9273 T11155 T11154 arg2Of isolated,were
R9274 T11155 T11156 arg1Of isolated,from
R9275 T11155 T11168 arg1Of isolated,and
R9276 T11157 T11156 arg2Of spleens,from
R9277 T11157 T11158 arg1Of spleens,of
R9278 T11157 T11161 arg1Of spleens,by
R9279 T11160 T11158 arg2Of mice,of
R9280 T11160 T11159 arg1Of mice,BALB/c
R9281 T11164 T11161 arg2Of kit,by
R9282 T11164 T11162 arg1Of kit,negative
R9283 T11164 T11163 arg1Of kit,selection
R9284 T11164 T11165 arg1Of kit,(
R9285 T11166 T11165 arg2Of Invitrogen,(
R9286 T11167 T11165 arg3Of ),(
R9287 T11171 T11172 arg1Of added,at
R9288 T11171 T11174 arg1Of added,and
R9289 T11173 T11172 arg2Of 2.5×105,at
R9290 T11174 T11168 arg2Of and,and
R9291 T11174 T11169 arg2Of and,were
R9292 T11174 T11170 arg1Of and,then
R9293 T11175 T11174 arg2Of co-cultured,and
R9294 T11175 T11176 arg1Of co-cultured,with
R9295 T11178 T11176 arg2Of BMDC,with
R9296 T11178 T11177 arg2Of BMDC,activated
R9297 T11181 T11179 arg2Of days,After
R9298 T11181 T11180 arg1Of days,5
R9299 T11183 T11184 arg1Of supernatant,was
R9300 T11183 T11185 arg2Of supernatant,collected
R9301 T11185 T11184 arg2Of collected,was
R9302 T11185 T11186 arg1Of collected,and
R9303 T11186 T11179 arg1Of and,After
R9304 T11186 T11182 arg1Of and,","
R9305 T11188 T11187 arg1Of secretion,the
R9306 T11188 T11189 arg1Of secretion,of
R9307 T11188 T11198 arg2Of secretion,measured
R9308 T11190 T11191 arg1Of IFNγ,","
R9309 T11191 T11193 arg1Of ",",","
R9310 T11192 T11191 arg2Of IL4,","
R9311 T11193 T11196 arg1Of ",",and
R9312 T11194 T11193 arg2Of IL2,","
R9313 T11196 T11189 arg2Of and,of
R9314 T11196 T11195 arg1Of and,","
R9315 T11197 T11196 arg2Of IL17A,and
R9316 T11198 T11186 arg2Of measured,and
R9317 T11200 T11198 arg1Of ELISA,measured
R9318 T11200 T11199 arg2Of ELISA,by
R9319 T11202 T11201 arg1Of controls,Negative
R9320 T11202 T11203 arg1Of controls,are
R9321 T11204 T11206 arg1Of 1,stimulated
R9322 T11206 T11203 arg2Of stimulated,are
R9323 T11206 T11205 arg1Of stimulated,)
R9324 T11206 T11208 arg1Of stimulated,without
R9325 T11207 T11206 arg2Of BMDC,stimulated
R9326 T11209 T11208 arg2Of co-culture,without
R9327 T11209 T11210 arg1Of co-culture,with
R9328 T11214 T11211 arg1Of cells,allogeneic
R9329 T11214 T11212 arg1Of cells,CD4+
R9330 T11214 T11213 arg1Of cells,T
R9331 T11214 T11215 arg1Of cells,;
R9332 T11215 T11210 arg2Of ;,with
R9333 T11216 T11217 arg1Of 2,)
R9334 T11221 T11215 arg2Of cells,;
R9335 T11221 T11216 arg1Of cells,2
R9336 T11221 T11218 arg1Of cells,allogeneic
R9337 T11221 T11219 arg1Of cells,CD4+
R9338 T11221 T11220 arg1Of cells,T
R9339 T11221 T11222 arg1Of cells,without
R9340 T11223 T11222 arg2Of co-culture,without
R9341 T11223 T11224 arg1Of co-culture,with
R9342 T11226 T11224 arg2Of BMDC,with
R9343 T11226 T11225 arg2Of BMDC,stimulated
R9344 T11229 T11227 arg1Of controls,Both
R9345 T11229 T11228 arg1Of controls,negative
R9346 T11229 T11230 arg1Of controls,show
R9347 T11232 T11230 arg2Of production,show
R9348 T11232 T11231 arg1Of production,no
R9349 T11232 T11233 arg1Of production,of
R9350 T11235 T11233 arg2Of cytokines,of
R9351 T11235 T11234 arg1Of cytokines,aforementioned
R9640 T11603 T11602 arg1Of expression,RNA
R9641 T11603 T11604 arg1Of expression,by
R9642 T11605 T11604 arg2Of BMDC,by
R9643 T11606 T11607 arg1Of BMDC,were
R9644 T11606 T11608 arg2Of BMDC,cultured
R9645 T11608 T11607 arg2Of cultured,were
R9646 T11608 T11609 arg1Of cultured,at
R9647 T11608 T11612 arg1Of cultured,in
R9648 T11608 T11619 arg1Of cultured,in
R9649 T11608 T11635 arg1Of cultured,After
R9650 T11611 T11609 arg2Of cells/mL,at
R9651 T11611 T11610 arg1Of cells/mL,106
R9652 T11614 T11612 arg2Of mL,in
R9653 T11614 T11613 arg1Of mL,10
R9654 T11614 T11615 arg1Of mL,of
R9655 T11618 T11615 arg2Of medium,of
R9656 T11618 T11616 arg1Of medium,RPMI-1640
R9657 T11618 T11617 arg1Of medium,complete
R9658 T11621 T11622 arg1Of presence,or
R9659 T11622 T11619 arg2Of or,in
R9660 T11622 T11620 arg1Of or,the
R9661 T11622 T11624 arg1Of or,of
R9662 T11622 T11633 arg1Of or,:
R9663 T11623 T11622 arg2Of absence,or
R9664 T11625 T11626 arg1Of 100,ng/mL
R9665 T11627 T11625 arg1Of LPS,100
R9666 T11627 T11628 arg1Of LPS,or
R9667 T11628 T11624 arg2Of or,of
R9668 T11631 T11628 arg2Of poly,or
R9669 T11631 T11629 arg1Of poly,25
R9670 T11631 T11630 arg1Of poly,µg/mL
R9671 T11631 T11632 arg1Of poly,I
R9672 T11634 T11633 arg2Of C.,:
R9673 T11636 T11637 arg1Of 1,and
R9674 T11638 T11637 arg2Of 2,and
R9675 T11639 T11635 arg2Of hours,After
R9676 T11639 T11636 arg1Of hours,1
R9677 T11639 T11638 arg1Of hours,2
R9678 T11641 T11642 arg1Of RNA,was
R9679 T11641 T11643 arg2Of RNA,isolated
R9680 T11643 T11607 modOf isolated,were
R9681 T11643 T11640 arg1Of isolated,","
R9682 T11643 T11642 arg2Of isolated,was
R9683 T11643 T11644 arg1Of isolated,with
R9684 T11643 T11649 arg1Of isolated,according
R9685 T11645 T11644 arg2Of TRI-reagent,with
R9686 T11645 T11646 arg1Of TRI-reagent,(
R9687 T11647 T11646 arg2Of Sigma,(
R9688 T11648 T11646 arg3Of ),(
R9689 T11650 T11649 arg2Of to,according
R9690 T11652 T11651 arg1Of manufacturer,the
R9691 T11652 T11653 arg2Of manufacturer,'s
R9692 T11654 T11650 arg2Of instructions,to
R9693 T11654 T11653 arg1Of instructions,'s
R9694 T11656 T11655 arg1Of expression,The
R9695 T11656 T11657 arg1Of expression,of
R9696 T11656 T11667 arg1Of expression,was
R9697 T11656 T11668 arg2Of expression,determined
R9698 T11658 T11659 arg1Of Il6,","
R9699 T11659 T11661 arg1Of ",",","
R9700 T11660 T11659 arg2Of Il12p40,","
R9701 T11661 T11664 arg1Of ",",and
R9702 T11662 T11661 arg2Of Gmcsf,","
R9703 T11664 T11657 arg2Of and,of
R9704 T11664 T11663 arg1Of and,","
R9705 T11666 T11664 arg2Of mRNA,and
R9706 T11666 T11665 arg1Of mRNA,Sharpin
R9707 T11668 T11667 arg2Of determined,was
R9708 T11670 T11668 arg1Of qRT-PCR,determined
R9709 T11670 T11669 arg2Of qRT-PCR,by
R9710 T11671 T11672 arg1Of Primers,and
R9711 T11672 T11674 arg1Of and,were
R9712 T11672 T11675 arg2Of and,purchased
R9713 T11673 T11672 arg2Of probes,and
R9714 T11675 T11674 arg2Of purchased,were
R9715 T11675 T11676 arg1Of purchased,from
R9716 T11675 T11721 arg1Of purchased,and
R9717 T11679 T11677 arg1Of Reverse,Applied
R9718 T11679 T11678 arg1Of Reverse,Biosystems.
R9719 T11679 T11701 arg1Of Reverse,containing
R9720 T11680 T11681 arg1Of transcription,was
R9721 T11680 T11682 arg2Of transcription,performed
R9722 T11682 T11681 arg2Of performed,was
R9723 T11682 T11683 arg1Of performed,at
R9724 T11682 T11685 arg1Of performed,for
R9725 T11682 T11688 arg1Of performed,with
R9726 T11684 T11683 arg2Of 42°C,at
R9727 T11687 T11685 arg2Of minutes,for
R9728 T11687 T11686 arg1Of minutes,60
R9729 T11692 T11688 arg2Of step,with
R9730 T11692 T11689 arg1Of step,the
R9731 T11692 T11690 arg1Of step,final
R9732 T11692 T11691 arg1Of step,denaturation
R9733 T11692 T11693 arg1Of step,at
R9734 T11692 T11695 arg1Of step,for
R9735 T11692 T11698 arg1Of step,in
R9736 T11694 T11693 arg2Of 90°C,at
R9737 T11697 T11695 arg2Of minutes,for
R9738 T11697 T11696 arg1Of minutes,5
R9739 T11700 T11698 arg2Of µl,in
R9740 T11700 T11699 arg1Of µl,30
R9741 T11701 T11681 modOf containing,was
R9742 T11703 T11701 arg2Of µg,containing
R9743 T11703 T11702 arg1Of µg,0.5
R9744 T11703 T11704 arg1Of µg,of
R9745 T11707 T11704 arg2Of dNTPs,of
R9746 T11707 T11705 arg1Of dNTPs,total
R9747 T11707 T11706 arg1Of dNTPs,RNA.
R9748 T11707 T11708 arg1Of dNTPs,","
R9749 T11709 T11708 arg2Of oligo,","
R9750 T11709 T11710 arg1Of oligo,(
R9751 T11711 T11710 arg2Of dT,(
R9752 T11712 T11710 arg3Of ),(
R9753 T11714 T11676 arg2Of primer,from
R9754 T11714 T11679 arg1Of primer,Reverse
R9755 T11714 T11713 arg1Of primer,15
R9756 T11714 T11715 arg1Of primer,","
R9757 T11719 T11715 arg2Of Inhibitor,","
R9758 T11719 T11716 arg1Of Inhibitor,recombinant
R9759 T11719 T11717 arg1Of Inhibitor,RNasin
R9760 T11719 T11718 arg1Of Inhibitor,Ribonuclease
R9761 T11721 T11720 arg1Of and,","
R9762 T11724 T11722 arg1Of Transcriptase,M-MLV
R9763 T11724 T11723 arg1Of Transcriptase,Reverse
R9764 T11724 T11725 arg1Of Transcriptase,(
R9765 T11724 T11734 arg1Of Transcriptase,were
R9766 T11724 T11735 arg2Of Transcriptase,used
R9767 T11726 T11725 arg2Of all,(
R9768 T11726 T11727 arg1Of all,from
R9769 T11726 T11729 arg1Of all,","
R9770 T11728 T11727 arg2Of Promega,from
R9771 T11730 T11729 arg2Of Madison,","
R9772 T11730 T11731 arg1Of Madison,","
R9773 T11732 T11731 arg2Of WI,","
R9774 T11733 T11725 arg3Of ),(
R9775 T11735 T11721 arg2Of used,and
R9776 T11735 T11734 arg2Of used,were
R9777 T11735 T11736 arg1Of used,according
R9778 T11737 T11736 arg2Of to,according
R9779 T11739 T11738 arg1Of manufacturer,the
R9780 T11739 T11740 arg2Of manufacturer,'s
R9781 T11741 T11737 arg2Of instruction,to
R9782 T11741 T11740 arg1Of instruction,'s
R9783 T11743 T11742 arg1Of transcription,Reverse
R9784 T11743 T11744 arg1Of transcription,was
R9785 T11743 T11745 arg2Of transcription,done
R9786 T11745 T11744 arg2Of done,was
R9787 T11745 T11746 arg1Of done,in
R9788 T11751 T11746 arg2Of Cycler,in
R9789 T11751 T11747 arg1Of Cycler,a
R9790 T11751 T11748 arg1Of Cycler,PTC-200
R9791 T11751 T11749 arg1Of Cycler,Peltier
R9792 T11751 T11750 arg1Of Cycler,Thermal
R9793 T11751 T11752 arg1Of Cycler,(
R9794 T11754 T11753 arg1Of Research,MJ
R9795 T11754 T11755 arg1Of Research,","
R9796 T11755 T11752 arg2Of ",",(
R9797 T11755 T11757 arg1Of ",",","
R9798 T11755 T11758 arg1Of ",",MA
R9799 T11756 T11755 arg2Of Watertown,","
R9800 T11759 T11752 arg3Of ),(
R9801 T11760 T11761 arg1Of . qRT-P,CR
R9802 T11760 T11762 arg2Of . qRT-P,was perfo
R9803 T11762 T11744 modOf was perfo,was
R9804 T11762 T11761 arg2Of was perfo,CR
R9805 T11762 T11763 arg1Of was perfo,rm
R9806 T11762 T11798 arg1Of was perfo,", Ifnβ, G"
R9807 T11769 T11763 arg2Of Detect,rm
R9808 T11769 T11764 arg1Of Detect,ed
R9809 T11769 T11765 arg1Of Detect,in AB
R9810 T11769 T11766 arg1Of Detect,I Pr
R9811 T11769 T11767 arg1Of Detect,ism 7700
R9812 T11769 T11768 arg1Of Detect,Sequence
R9813 T11769 T11770 arg1Of Detect,ion
R9814 T11769 T11784 arg1Of Detect,"s, "
R9815 T11772 T11771 arg1Of with,System
R9816 T11774 T11770 arg2Of ne Exp,ion
R9817 T11774 T11772 arg1Of ne Exp,with
R9818 T11774 T11773 arg1Of ne Exp,TaqMan® Ge
R9819 T11774 T11775 arg1Of ne Exp,r
R9820 T11777 T11775 arg2Of Assays (Ap,r
R9821 T11777 T11776 arg1Of Assays (Ap,ession
R9822 T11777 T11778 arg1Of Assays (Ap,p
R9823 T11780 T11778 arg2Of iosy,p
R9824 T11780 T11779 arg1Of iosy,lied B
R9825 T11780 T11781 arg1Of iosy,s
R9826 T11782 T11781 arg2Of te,s
R9827 T11783 T11775 arg3Of m,r
R9828 T11786 T11785 arg1Of r Ci,Foste
R9829 T11786 T11787 arg1Of r Ci,t
R9830 T11787 T11789 arg1Of t,C
R9831 T11788 T11787 arg2Of "y, ",t
R9832 T11789 T11791 arg1Of C,m
R9833 T11790 T11789 arg2Of A) for ,C
R9834 T11791 T11793 arg1Of m,A
R9835 T11792 T11791 arg2Of ouse,m
R9836 T11793 T11796 arg1Of A,"L6,"
R9837 T11794 T11793 arg2Of "ctb, ",A
R9838 T11796 T11784 arg2Of "L6,","s, "
R9839 T11796 T11795 arg1Of "L6,",I
R9840 T11797 T11796 arg2Of IL12p40,"L6,"
R9841 T11799 T11798 arg2Of mc,", Ifnβ, G"
R9842 T11801 T11800 arg1Of and Sharpin ,"sf,"
R9843 T11801 T11802 arg2Of and Sharpin ,ac
R9844 T11803 T11799 arg2Of cording ,mc
R9845 T11803 T11802 arg1Of cording ,ac
R9846 T11806 T11804 arg1Of standard,The
R9847 T11806 T11805 arg1Of standard,endogenous
R9848 T11806 T11807 arg1Of standard,for
R9849 T11806 T11813 arg1Of standard,was
R9850 T11806 T11814 arg1Of standard,β-actin
R9851 T11808 T11807 arg2Of normalization,for
R9852 T11808 T11809 arg1Of normalization,of
R9853 T11812 T11809 arg2Of gene,of
R9854 T11812 T11810 arg1Of gene,the
R9855 T11812 T11811 arg1Of gene,target
R9856 T11814 T11813 arg2Of β-actin,was
R9857 T11817 T11815 arg1Of expression,Relative
R9858 T11817 T11816 arg1Of expression,gene
R9859 T11817 T11818 arg1Of expression,was
R9860 T11817 T11819 arg2Of expression,calculated
R9861 T11819 T11818 arg2Of calculated,was
R9862 T11819 T11820 modOf calculated,using
R9863 T11820 T11824 arg1Of using,[
R9864 T11823 T11820 arg2Of method,using
R9865 T11823 T11821 arg1Of method,the
R9866 T11823 T11822 arg1Of method,2−ΔΔCt
R9867 T11825 T11824 arg2Of 56,[
R9868 T11826 T11824 arg3Of ],[
R9960 T12002 T12001 arg1Of vitro,In
R9961 T12004 T12002 arg1Of secretion,vitro
R9962 T12004 T12003 arg1Of secretion,cytokine
R9963 T12004 T12005 arg1Of secretion,by
R9964 T12006 T12005 arg2Of BMDC,by
R9965 T12008 T12007 arg1Of BMDC,The
R9966 T12008 T12009 arg1Of BMDC,were
R9967 T12008 T12010 arg2Of BMDC,cultured
R9968 T12010 T12009 arg2Of cultured,were
R9969 T12010 T12011 arg1Of cultured,in
R9970 T12010 T12024 arg1Of cultured,","
R9971 T12010 T12025 arg1Of cultured,respectively
R9972 T12010 T12026 arg1Of cultured,","
R9973 T12010 T12027 arg1Of cultured,in
R9974 T12013 T12011 arg2Of wells,in
R9975 T12013 T12012 arg1Of wells,triplicate
R9976 T12013 T12014 arg1Of wells,of
R9977 T12015 T12016 arg1Of 24-well,or
R9978 T12017 T12016 arg2Of 96-well,or
R9979 T12018 T12014 arg2Of plates,of
R9980 T12018 T12015 arg1Of plates,24-well
R9981 T12018 T12017 arg1Of plates,96-well
R9982 T12018 T12019 arg1Of plates,at
R9983 T12020 T12021 arg1Of 106,or
R9984 T12022 T12021 arg2Of 105,or
R9985 T12023 T12019 arg2Of cells/mL,at
R9986 T12023 T12020 arg1Of cells/mL,106
R9987 T12023 T12022 arg1Of cells/mL,105
R9988 T12030 T12027 arg2Of medium,in
R9989 T12030 T12028 arg1Of medium,RPMI-1640
R9990 T12030 T12029 arg1Of medium,complete
R9991 T12032 T12031 arg1Of cells,The
R9992 T12032 T12033 arg1Of cells,were
R9993 T12032 T12034 arg2Of cells,washed
R9994 T12032 T12036 arg2Of cells,stimulated
R9995 T12034 T12035 arg1Of washed,and
R9996 T12035 T12033 arg2Of and,were
R9997 T12035 T12048 arg1Of and,After
R9998 T12035 T12051 arg1Of and,","
R9999 T12036 T12035 arg2Of stimulated,and
R10000 T12036 T12037 arg1Of stimulated,with
R10001 T12038 T12039 arg1Of 100,ng/mL
R10002 T12040 T12038 arg1Of LPS,100
R10003 T12040 T12041 arg1Of LPS,or
R10004 T12041 T12037 arg2Of or,with
R10005 T12041 T12046 arg1Of or,:
R10006 T12044 T12041 arg2Of poly,or
R10007 T12044 T12042 arg1Of poly,25
R10008 T12044 T12043 arg1Of poly,µg/mL
R10009 T12044 T12045 arg1Of poly,I
R10010 T12047 T12046 arg2Of C.,:
R10011 T12050 T12048 arg2Of hours,After
R10012 T12050 T12049 arg1Of hours,24
R10013 T12052 T12053 arg1Of supernatants,were
R10014 T12052 T12054 arg2Of supernatants,harvested
R10015 T12054 T12051 arg2Of harvested,","
R10016 T12054 T12053 arg2Of harvested,were
R10017 T12054 T12055 arg1Of harvested,for
R10018 T12057 T12055 arg2Of analysis,for
R10019 T12057 T12056 arg1Of analysis,ELISA
R10020 T12059 T12058 arg1Of presence,The
R10021 T12059 T12060 arg1Of presence,of
R10022 T12059 T12062 arg1Of presence,in
R10023 T12059 T12065 arg1Of presence,was
R10024 T12059 T12066 arg2Of presence,determined
R10025 T12061 T12060 arg2Of nitrite,of
R10026 T12064 T12062 arg2Of supernatants,in
R10027 T12064 T12063 arg1Of supernatants,the
R10028 T12066 T12065 arg2Of determined,was
R10029 T12067 T12066 arg3Of using,determined
R10030 T12070 T12067 arg2Of reagent,using
R10031 T12070 T12068 arg1Of reagent,the
R10032 T12070 T12069 arg1Of reagent,Griess
R10170 T12228 T12227 arg1Of hours,48
R10171 T12229 T12228 arg1Of later,hours
R10172 T12231 T12232 arg1Of cells,were
R10173 T12231 T12233 arg2Of cells,washed
R10174 T12233 T12229 arg1Of washed,later
R10175 T12233 T12230 arg1Of washed,","
R10176 T12233 T12232 arg2Of washed,were
R10177 T12233 T12234 arg1Of washed,briefly
R10178 T12233 T12235 arg1Of washed,with
R10179 T12236 T12235 arg2Of PBS,with
R10180 T12236 T12237 arg1Of PBS,","
R10181 T12236 T12239 arg2Of PBS,fixed
R10182 T12239 T12238 arg1Of fixed,then
R10183 T12239 T12240 arg1Of fixed,in
R10184 T12242 T12240 arg2Of methanol,in
R10185 T12242 T12241 arg1Of methanol,cold
R10186 T12242 T12243 arg1Of methanol,at
R10187 T12244 T12243 arg2Of −20°C,at
R10188 T12244 T12245 arg1Of −20°C,for
R10189 T12244 T12251 arg1Of −20°C,at
R10190 T12247 T12246 arg1Of minutes,10
R10191 T12247 T12248 arg1Of minutes,and
R10192 T12248 T12245 arg2Of and,for
R10193 T12250 T12248 arg2Of acetone,and
R10194 T12250 T12249 arg1Of acetone,cold
R10195 T12252 T12251 arg2Of -20°C,at
R10196 T12252 T12253 arg1Of -20°C,for
R10197 T12255 T12253 arg2Of minute,for
R10198 T12255 T12254 arg1Of minute,1
R10199 T12257 T12256 arg1Of cells,Incubate
R10200 T12257 T12258 arg1Of cells,with
R10201 T12257 T12262 arg1Of cells,for
R10202 T12261 T12258 arg2Of BSA,with
R10203 T12261 T12259 arg1Of BSA,PBS+1
R10204 T12261 T12260 arg1Of BSA,%
R10205 T12264 T12262 arg2Of minutes,for
R10206 T12264 T12263 arg1Of minutes,15
R10207 T12264 T12266 arg1Of minutes,block
R10208 T12266 T12262 arg3Of block,for
R10209 T12266 T12265 arg1Of block,to
R10210 T12268 T12266 arg2Of binding,block
R10211 T12268 T12267 arg1Of binding,non-specific
R10212 T12270 T12269 arg2Of cells,Fixed
R10213 T12270 T12271 arg1Of cells,were
R10214 T12270 T12273 arg2Of cells,incubated
R10215 T12273 T12271 arg2Of incubated,were
R10216 T12273 T12272 arg1Of incubated,then
R10217 T12273 T12274 arg1Of incubated,with
R10218 T12273 T12279 arg1Of incubated,as
R10219 T12273 T12289 arg1Of incubated,","
R10220 T12273 T12290 modOf incubated,followed
R10221 T12275 T12274 arg2Of anti-FLAG,with
R10222 T12275 T12276 arg1Of anti-FLAG,(
R10223 T12277 T12276 arg2Of 1∶100,(
R10224 T12278 T12276 arg3Of ),(
R10225 T12282 T12279 arg2Of antibody,as
R10226 T12282 T12280 arg1Of antibody,the
R10227 T12282 T12281 arg1Of antibody,primary
R10228 T12282 T12283 arg1Of antibody,at
R10229 T12285 T12283 arg2Of temperature,at
R10230 T12285 T12284 arg1Of temperature,room
R10231 T12285 T12286 arg1Of temperature,for
R10232 T12288 T12286 arg2Of hour,for
R10233 T12288 T12287 arg1Of hour,1
R10234 T12294 T12290 arg1Of washes,followed
R10235 T12294 T12291 arg2Of washes,by
R10236 T12294 T12292 arg1Of washes,three
R10237 T12294 T12293 arg1Of washes,5-minute
R10238 T12295 T12296 arg1Of Cells,were
R10239 T12295 T12298 arg2Of Cells,incubated
R10240 T12298 T12296 arg2Of incubated,were
R10241 T12298 T12297 arg1Of incubated,further
R10242 T12298 T12299 arg1Of incubated,with
R10243 T12298 T12306 arg1Of incubated,as
R10244 T12298 T12316 arg1Of incubated,","
R10245 T12298 T12317 modOf incubated,followed
R10246 T12302 T12299 arg2Of IgG-FITC,with
R10247 T12302 T12300 arg1Of IgG-FITC,goat
R10248 T12302 T12301 arg1Of IgG-FITC,anti-rabbit
R10249 T12302 T12303 arg1Of IgG-FITC,(
R10250 T12304 T12303 arg2Of 1∶100,(
R10251 T12305 T12303 arg3Of ),(
R10252 T12309 T12306 arg2Of antibody,as
R10253 T12309 T12307 arg1Of antibody,the
R10254 T12309 T12308 arg1Of antibody,secondary
R10255 T12309 T12310 arg1Of antibody,at
R10256 T12312 T12310 arg2Of temperature,at
R10257 T12312 T12311 arg1Of temperature,room
R10258 T12312 T12313 arg1Of temperature,for
R10259 T12315 T12313 arg2Of minutes,for
R10260 T12315 T12314 arg1Of minutes,30
R10261 T12321 T12317 arg1Of washes,followed
R10262 T12321 T12318 arg2Of washes,by
R10263 T12321 T12319 arg1Of washes,three
R10264 T12321 T12320 arg1Of washes,5-minute
R10265 T12322 T12323 arg1Of Cells,were
R10266 T12322 T12325 arg2Of Cells,examined
R10267 T12325 T12323 arg2Of examined,were
R10268 T12325 T12324 arg1Of examined,then
R10269 T12325 T12326 modOf examined,using
R10270 T12329 T12326 arg2Of microscope,using
R10271 T12329 T12327 arg1Of microscope,inverse
R10272 T12329 T12328 arg1Of microscope,fluorescence
R10390 T12505 T12504 arg2Of adding,After
R10391 T12505 T12517 arg1Of adding,:
R10392 T12505 T12518 arg1Of adding,C
R10393 T12507 T12506 arg1Of ligands,stimulating
R10394 T12507 T12508 arg1Of ligands,","
R10398 T12511 T12508 arg2Of LPS,","
R10399 T12512 T12505 arg2Of or,adding
R10400 T12516 T12512 arg2Of I,or
R10401 T12516 T12513 arg1Of I,25
R10402 T12516 T12514 arg1Of I,µg/mL
R10403 T12516 T12515 arg1Of I,poly
R10404 T12520 T12505 arg1Of BMDC,adding
R10405 T12520 T12521 arg1Of BMDC,were
R10406 T12520 T12522 arg2Of BMDC,collected
R10407 T12520 T12524 arg2Of BMDC,lysed
R10408 T12522 T12523 arg1Of collected,and
R10409 T12523 T12504 arg1Of and,After
R10410 T12523 T12519 arg1Of and,","
R10411 T12523 T12521 arg2Of and,were
R10412 T12523 T12525 arg1Of and,at
R10413 T12524 T12523 arg2Of lysed,and
R10414 T12526 T12527 arg1Of 0-,","
R10415 T12528 T12527 arg2Of 15-,","
R10416 T12530 T12532 arg1Of 30-,and
R10417 T12532 T12531 arg1Of and,","
R10418 T12533 T12532 arg2Of 60-minute,and
R10419 T12535 T12525 arg2Of points,at
R10420 T12535 T12526 arg1Of points,0-
R10421 T12535 T12528 arg1Of points,15-
R10422 T12535 T12529 arg1Of points,","
R10423 T12535 T12530 arg1Of points,30-
R10424 T12535 T12533 arg1Of points,60-minute
R10425 T12535 T12534 arg1Of points,time
R10426 T12536 T12537 arg1Of Immunoblots,were
R10427 T12536 T12538 arg2Of Immunoblots,performed
R10428 T12538 T12537 arg2Of performed,were
R10429 T12538 T12539 arg1Of performed,with
R10430 T12540 T12539 arg2Of antibodies,with
R10431 T12540 T12541 arg1Of antibodies,(
R10432 T12540 T12546 arg1Of antibodies,against
R10433 T12544 T12541 arg2Of Technology,(
R10434 T12544 T12542 arg1Of Technology,Cell
R10435 T12544 T12543 arg1Of Technology,Signaling
R10436 T12545 T12541 arg3Of ),(
R10437 T12547 T12548 arg1Of p-IKK1/2,(
R10438 T12547 T12552 arg1Of p-IKK1/2,","
R10439 T12550 T12548 arg2Of 2697,(
R10440 T12550 T12549 arg1Of 2697,#
R10441 T12551 T12548 arg3Of ),(
R10442 T12552 T12558 arg1Of ",",","
R10443 T12553 T12552 arg2Of p-IκBα,","
R10444 T12553 T12554 arg1Of p-IκBα,(
R10445 T12556 T12554 arg2Of 9246,(
R10446 T12556 T12555 arg1Of 9246,#
R10447 T12557 T12554 arg3Of ),(
R10448 T12558 T12546 arg2Of ",",against
R10449 T12559 T12560 arg1Of IκBα,(
R10450 T12559 T12564 arg1Of IκBα,","
R10451 T12562 T12560 arg2Of 4814,(
R10452 T12562 T12561 arg1Of 4814,#
R10453 T12563 T12560 arg3Of ),(
R10454 T12564 T12570 arg1Of ",",","
R10455 T12565 T12564 arg2Of p-TBK1,","
R10456 T12565 T12566 arg1Of p-TBK1,(
R10457 T12568 T12566 arg2Of 5483,(
R10458 T12568 T12567 arg1Of 5483,#
R10459 T12569 T12566 arg3Of ),(
R10460 T12570 T12576 arg1Of ",",","
R10461 T12571 T12570 arg2Of p-p38,","
R10462 T12571 T12572 arg1Of p-p38,(
R10463 T12574 T12572 arg2Of 9216,(
R10464 T12574 T12573 arg1Of 9216,#
R10465 T12575 T12572 arg3Of ),(
R10466 T12576 T12558 arg2Of ",",","
R10467 T12577 T12578 arg1Of p38,(
R10468 T12577 T12582 arg1Of p38,","
R10469 T12580 T12578 arg2Of 9212,(
R10470 T12580 T12579 arg1Of 9212,#
R10471 T12581 T12578 arg3Of ),(
R10472 T12582 T12589 arg1Of ",",and
R10473 T12583 T12582 arg2Of p-ERK1/2,","
R10474 T12583 T12584 arg1Of p-ERK1/2,(
R10475 T12586 T12584 arg2Of 4376,(
R10476 T12586 T12585 arg1Of 4376,#
R10477 T12587 T12584 arg3Of ),(
R10478 T12589 T12576 arg2Of and,","
R10479 T12589 T12588 arg1Of and,","
R10480 T12590 T12589 arg2Of ERK1/2,and
R10481 T12590 T12591 arg1Of ERK1/2,(
R10482 T12593 T12591 arg2Of 4695,(
R10483 T12593 T12592 arg1Of 4695,#
R10484 T12594 T12591 arg3Of ),(
R10485 T12595 T12596 arg1Of Beta-actin,(
R10486 T12595 T12603 arg1Of Beta-actin,was
R10487 T12595 T12604 arg2Of Beta-actin,used
R10488 T12597 T12596 arg2Of sc-47778,(
R10489 T12597 T12598 arg1Of sc-47778,","
R10490 T12601 T12598 arg2Of Biotechnology,","
R10491 T12601 T12599 arg1Of Biotechnology,Santa
R10492 T12601 T12600 arg1Of Biotechnology,Cruz
R10493 T12602 T12596 arg3Of ),(
R10494 T12604 T12603 arg2Of used,was
R10495 T12604 T12605 arg1Of used,as
R10496 T12606 T12605 arg2Of loading,as
R10497 T12607 T12606 arg2Of control,loading
R10525 T12710 T12709 arg1Of analysis,Statistical
R10526 T12711 T12712 arg1Of Data,are
R10527 T12711 T12713 arg2Of Data,expressed
R10528 T12713 T12712 arg2Of expressed,are
R10529 T12713 T12714 arg1Of expressed,as
R10530 T12717 T12714 arg2Of SD,as
R10531 T12717 T12715 arg1Of SD,mean
R10532 T12717 T12716 arg1Of SD,±
R10533 T12720 T12718 arg1Of significance,The
R10534 T12720 T12719 arg1Of significance,statistical
R10535 T12720 T12721 arg1Of significance,of
R10536 T12720 T12728 arg1Of significance,was
R10537 T12720 T12729 arg2Of significance,determined
R10538 T12722 T12721 arg2Of differences,of
R10539 T12722 T12723 arg1Of differences,of
R10540 T12722 T12725 arg1Of differences,between
R10541 T12724 T12723 arg2Of means,of
R10542 T12727 T12725 arg2Of groups,between
R10543 T12727 T12726 arg1Of groups,experimental
R10544 T12729 T12728 arg2Of determined,was
R10545 T12731 T12732 arg2Of Students,'
R10546 T12733 T12729 arg1Of t-test,determined
R10547 T12733 T12730 arg2Of t-test,by
R10548 T12733 T12732 arg1Of t-test,'
R10778 T12992 T12991 arg1Of vivo,In
R10779 T12992 T12993 arg1Of vivo,and
R10780 T12995 T12993 arg2Of vitro,and
R10781 T12995 T12994 arg1Of vitro,in
R10782 T12996 T12992 arg1Of features,vivo
R10783 T12996 T12995 arg1Of features,vitro
R10784 T12996 T12997 arg1Of features,of
R10785 T12998 T12997 arg2Of SHARPIN,of
R10786 T13000 T12999 arg2Of A,(
R10787 T13001 T12999 arg3Of ),(
R10788 T13002 T13003 arg1Of COILS,and
R10789 T13004 T13003 arg2Of MotifScan,and
R10790 T13005 T12999 arg1Of programs,(
R10791 T13005 T13002 arg1Of programs,COILS
R10792 T13005 T13004 arg1Of programs,MotifScan
R10793 T13005 T13006 arg1Of programs,were
R10794 T13005 T13007 arg2Of programs,used
R10795 T13007 T13006 arg2Of used,were
R10796 T13009 T13007 arg3Of predict,used
R10797 T13009 T13008 arg1Of predict,to
R10798 T13011 T13009 arg2Of presence,predict
R10799 T13011 T13010 arg1Of presence,the
R10800 T13011 T13012 arg1Of presence,of
R10801 T13015 T13014 arg2Of coiled-coil,(
R10802 T13016 T13014 arg3Of ),(
R10803 T13017 T13013 arg1Of domain,CC
R10804 T13017 T13014 arg1Of domain,(
R10805 T13017 T13018 arg1Of domain,","
R10806 T13018 T13024 arg1Of ",",and
R10807 T13021 T13020 arg2Of ubiquitin-like,(
R10808 T13022 T13020 arg3Of ),(
R10809 T13023 T13018 arg2Of domain,","
R10810 T13023 T13019 arg1Of domain,UBL
R10811 T13023 T13020 arg1Of domain,(
R10812 T13024 T13012 arg2Of and,of
R10813 T13024 T13033 arg1Of and,which
R10814 T13024 T13034 arg1Of and,form
R10815 T13025 T13026 arg1Of ZFRBP,(
R10816 T13029 T13026 arg2Of protein,(
R10817 T13029 T13027 arg1Of protein,zinc-finger
R10818 T13029 T13028 arg1Of protein,Ran-Binding
R10819 T13029 T13030 arg1Of protein,2
R10820 T13031 T13026 arg3Of ),(
R10821 T13032 T13024 arg2Of domain,and
R10822 T13032 T13025 arg1Of domain,ZFRBP
R10823 T13037 T13034 arg2Of patterns,form
R10824 T13037 T13035 arg1Of patterns,similar
R10825 T13037 T13036 arg1Of patterns,motif
R10826 T13037 T13038 arg1Of patterns,in
R10827 T13037 T13042 arg1Of patterns,of
R10828 T13041 T13038 arg2Of protein,in
R10829 T13041 T13039 arg1Of protein,the
R10830 T13041 T13040 arg1Of protein,SHARPIN
R10831 T13043 T13044 arg1Of human,","
R10832 T13045 T13046 arg1Of mouse,and
R10833 T13046 T13044 arg2Of and,","
R10834 T13048 T13046 arg2Of origins.,and
R10835 T13048 T13047 arg1Of origins.,rat
R10836 T13048 T13049 arg1Of origins.,(
R10837 T13050 T13049 arg2Of B,(
R10838 T13051 T13049 arg3Of ),(
R10839 T13054 T13042 arg2Of females,of
R10840 T13054 T13043 arg1Of females,human
R10841 T13054 T13045 arg1Of females,mouse
R10842 T13054 T13048 arg1Of females,origins.
R10843 T13054 T13052 arg1Of females,Eight
R10844 T13054 T13053 arg1Of females,week-old
R10845 T13054 T13055 arg1Of females,of
R10846 T13056 T13057 arg1Of WT,and
R10847 T13058 T13057 arg2Of cpdm,and
R10848 T13059 T13055 arg2Of mice,of
R10849 T13059 T13056 arg1Of mice,WT
R10850 T13059 T13058 arg1Of mice,cpdm
R10851 T13062 T13060 arg1Of mice,The
R10852 T13062 T13061 arg1Of mice,mutant
R10853 T13062 T13066 arg1Of mice,develop
R10854 T13066 T13063 arg1Of develop,(
R10855 T13066 T13064 arg1Of develop,above
R10856 T13066 T13065 arg1Of develop,)
R10857 T13069 T13066 arg2Of inflammation,develop
R10858 T13069 T13067 arg1Of inflammation,progressive
R10859 T13069 T13068 arg1Of inflammation,skin
R10860 T13069 T13070 arg1Of inflammation,starting
R10861 T13070 T13071 arg1Of starting,at
R10862 T13073 T13072 arg1Of four,about
R10863 T13074 T13073 arg1Of weeks.,four
R10864 T13074 T13075 arg1Of weeks.,(
R10865 T13076 T13075 arg2Of C,(
R10866 T13077 T13075 arg3Of ),(
R10867 T13078 T13074 arg1Of Extramedullary,weeks.
R10868 T13080 T13071 arg2Of causes,at
R10869 T13080 T13078 arg1Of causes,Extramedullary
R10870 T13080 T13079 arg1Of causes,hematopoiesis
R10871 T13080 T13093 modOf causes,were
R10872 T13082 T13081 arg1Of enlargement,marked
R10873 T13082 T13083 arg1Of enlargement,of
R10874 T13082 T13093 arg1Of enlargement,were
R10875 T13082 T13094 arg2Of enlargement,incubated
R10876 T13085 T13083 arg2Of spleen,of
R10877 T13085 T13084 arg1Of spleen,the
R10878 T13085 T13086 arg1Of spleen,of
R10879 T13088 T13087 arg1Of mice.,cpdm
R10880 T13088 T13089 arg1Of mice.,(
R10881 T13090 T13089 arg2Of D,(
R10882 T13091 T13089 arg3Of ),(
R10883 T13092 T13086 arg2Of BMDC,of
R10884 T13092 T13088 arg1Of BMDC,mice.
R10885 T13094 T13093 arg2Of incubated,were
R10886 T13094 T13095 arg1Of incubated,in
R10887 T13097 T13098 arg1Of absence,or
R10888 T13098 T13095 arg2Of or,in
R10889 T13098 T13096 arg1Of or,the
R10890 T13098 T13100 arg1Of or,of
R10891 T13099 T13098 arg2Of presence,or
R10892 T13101 T13102 arg1Of 100,ng/ml
R10893 T13103 T13100 arg2Of LPS,of
R10894 T13103 T13101 arg1Of LPS,100
R10895 T13103 T13104 arg1Of LPS,for
R10896 T13106 T13104 arg2Of hours,for
R10897 T13106 T13105 arg1Of hours,4
R10898 T13109 T13107 arg1Of level,The
R10899 T13109 T13108 arg1Of level,mRNA
R10900 T13109 T13110 arg1Of level,of
R10901 T13109 T13112 arg1Of level,was
R10902 T13109 T13113 arg2Of level,measured
R10903 T13109 T13117 arg1Of level,presented
R10904 T13111 T13110 arg2Of Sharpin,of
R10905 T13113 T13112 arg2Of measured,was
R10906 T13113 T13114 arg1Of measured,by
R10907 T13113 T13116 arg1Of measured,and
R10908 T13115 T13114 arg2Of qRT-PCR,by
R10909 T13117 T13116 arg2Of presented,and
R10910 T13117 T13119 arg1Of presented,to
R10911 T13119 T13118 arg1Of to,relative
R10912 T13122 T13119 arg2Of expression,to
R10913 T13122 T13120 arg1Of expression,the
R10914 T13122 T13121 arg1Of expression,mRNA
R10915 T13122 T13123 arg1Of expression,in
R10916 T13126 T13123 arg2Of BMDC,in
R10917 T13126 T13124 arg1Of BMDC,non-stimulated
R10918 T13126 T13125 arg1Of BMDC,WT
R10919 T13127 T13128 arg1Of Bars,represent
R10920 T13128 T13140 arg1Of represent,;
R10921 T13132 T13128 arg2Of s.d.,represent
R10922 T13132 T13129 arg1Of s.d.,the
R10923 T13132 T13130 arg1Of s.d.,mean
R10924 T13132 T13131 arg1Of s.d.,±
R10925 T13132 T13133 arg1Of s.d.,of
R10926 T13139 T13133 arg2Of 0.05,of
R10927 T13139 T13134 arg1Of 0.05,3
R10928 T13139 T13135 arg1Of 0.05,mice.
R10929 T13139 T13136 arg1Of 0.05,*
R10930 T13139 T13137 arg1Of 0.05,P
R10931 T13139 T13138 arg1Of 0.05,<
R10932 T13144 T13143 arg1Of 0.001.,<
R10933 T13146 T13145 arg2Of E,(
R10934 T13147 T13145 arg3Of ),(
R10935 T13148 T13141 arg1Of Fibroblast,**
R10936 T13148 T13142 arg1Of Fibroblast,P
R10937 T13148 T13144 arg1Of Fibroblast,0.001.
R10938 T13148 T13145 arg1Of Fibroblast,(
R10939 T13148 T13149 arg1Of Fibroblast,(
R10940 T13148 T13152 arg1Of Fibroblast,and
R10941 T13150 T13149 arg2Of NIH3T3,(
R10942 T13151 T13149 arg3Of ),(
R10943 T13152 T13154 arg1Of and,(
R10944 T13153 T13152 arg2Of macrophages,and
R10945 T13155 T13154 arg2Of RAW264.7,(
R10946 T13156 T13154 arg3Of ),(
R10947 T13157 T13148 arg1Of cells,Fibroblast
R10948 T13157 T13153 arg1Of cells,macrophages
R10949 T13157 T13158 arg1Of cells,were
R10950 T13157 T13159 arg2Of cells,transfected
R10951 T13159 T13140 arg2Of transfected,;
R10952 T13159 T13158 arg2Of transfected,were
R10953 T13159 T13160 arg1Of transfected,with
R10954 T13164 T13160 arg2Of pFLAG-SHARPIN,with
R10955 T13164 T13161 arg1Of pFLAG-SHARPIN,the
R10956 T13164 T13162 arg1Of pFLAG-SHARPIN,expression
R10957 T13164 T13163 arg1Of pFLAG-SHARPIN,plasmid
R10958 T13167 T13165 arg2Of hours,After
R10959 T13167 T13166 arg1Of hours,48
R10960 T13169 T13170 arg1Of cells,were
R10961 T13169 T13171 arg2Of cells,fixed
R10962 T13169 T13173 arg2Of cells,probed
R10963 T13171 T13172 arg1Of fixed,and
R10964 T13172 T13165 arg1Of and,After
R10965 T13172 T13168 arg1Of and,","
R10966 T13172 T13170 arg2Of and,were
R10967 T13173 T13172 arg2Of probed,and
R10968 T13173 T13174 arg1Of probed,with
R10969 T13175 T13176 arg1Of anti-FLAG,and
R10970 T13177 T13176 arg2Of FITC-conjugated,and
R10971 T13179 T13174 arg2Of antibody,with
R10972 T13179 T13175 arg1Of antibody,anti-FLAG
R10973 T13179 T13177 arg1Of antibody,FITC-conjugated
R10974 T13179 T13178 arg1Of antibody,secondary
R10975 T13180 T13181 arg1Of Nuclei,were
R10976 T13180 T13182 arg2Of Nuclei,stained
R10977 T13182 T13181 arg2Of stained,were
R10978 T13182 T13183 arg1Of stained,with
R10979 T13184 T13183 arg2Of DAPI,with
R10980 T13189 T13185 arg2Of lines,In
R10981 T13189 T13186 arg1Of lines,both
R10982 T13189 T13187 arg2Of lines,transfected
R10983 T13189 T13188 arg1Of lines,cell
R10984 T13191 T13192 arg1Of FLAG-SHARPIN,was
R10985 T13191 T13193 arg2Of FLAG-SHARPIN,found
R10986 T13191 T13195 arg1Of FLAG-SHARPIN,be
R10987 T13191 T13196 arg1Of FLAG-SHARPIN,cytoplasm-localized
R10988 T13193 T13185 arg1Of found,In
R10989 T13193 T13190 arg1Of found,","
R10990 T13193 T13192 arg2Of found,was
R10991 T13195 T13193 arg3Of be,found
R10992 T13195 T13194 arg1Of be,to
R10993 T13196 T13195 arg2Of cytoplasm-localized,be
R11269 T13537 T13538 arg1Of Effect,of
R11270 T13537 T13541 arg1Of Effect,on
R11271 T13540 T13538 arg2Of mutation,of
R11272 T13540 T13539 arg1Of mutation,Sharpin
R11273 T13543 T13542 arg1Of subpopulations,DC
R11274 T13543 T13544 arg1Of subpopulations,and
R11275 T13544 T13541 arg2Of and,on
R11276 T13545 T13544 arg2Of maturation,and
R11277 T13547 T13546 arg2Of A,(
R11278 T13548 T13546 arg3Of ),(
R11279 T13549 T13546 arg1Of Spleens,(
R11280 T13549 T13550 arg1Of Spleens,from
R11281 T13549 T13555 arg1Of Spleens,were
R11282 T13549 T13556 arg2Of Spleens,isolated
R11283 T13549 T13558 arg1Of Spleens,subject
R11284 T13551 T13552 arg1Of WT,and
R11285 T13553 T13552 arg2Of cpdm,and
R11286 T13554 T13550 arg2Of mice,from
R11287 T13554 T13551 arg1Of mice,WT
R11288 T13554 T13553 arg1Of mice,cpdm
R11289 T13556 T13557 arg1Of isolated,and
R11290 T13557 T13555 arg2Of and,were
R11291 T13558 T13557 arg2Of subject,and
R11292 T13558 T13559 arg1Of subject,to
R11293 T13560 T13561 arg1Of collagenase,and
R11294 T13561 T13559 arg2Of and,to
R11295 T13563 T13561 arg2Of digestion,and
R11296 T13563 T13562 arg1Of digestion,DNase
R11297 T13567 T13564 arg1Of homogenates,The
R11298 T13567 T13565 arg2Of homogenates,obtained
R11299 T13567 T13566 arg1Of homogenates,splenic
R11300 T13567 T13568 arg1Of homogenates,were
R11301 T13567 T13569 arg2Of homogenates,centrifuged
R11302 T13569 T13568 arg2Of centrifuged,were
R11303 T13569 T13570 arg1Of centrifuged,over
R11304 T13573 T13570 arg2Of gradient,over
R11305 T13573 T13571 arg1Of gradient,a
R11306 T13573 T13572 arg1Of gradient,Percoll
R11307 T13573 T13574 arg1Of gradient,(
R11308 T13573 T13582 arg1Of gradient,for
R11309 T13576 T13575 arg1Of %,35
R11310 T13576 T13577 arg1Of %,and
R11311 T13577 T13579 arg1Of and,%
R11312 T13578 T13577 arg2Of 55,and
R11313 T13580 T13574 arg2Of density,(
R11314 T13580 T13576 arg1Of density,%
R11315 T13580 T13578 arg1Of density,55
R11316 T13581 T13574 arg3Of ),(
R11317 T13584 T13582 arg2Of minutes,for
R11318 T13584 T13583 arg1Of minutes,15
R11319 T13586 T13585 arg1Of bands,The
R11320 T13586 T13587 arg1Of bands,at
R11321 T13586 T13597 arg1Of bands,were
R11322 T13586 T13598 arg2Of bands,pooled
R11323 T13586 T13600 arg2Of bands,washed
R11324 T13586 T13602 arg2Of bands,stained
R11325 T13589 T13590 arg1Of 35,%
R11326 T13591 T13588 arg1Of -medium,the
R11327 T13591 T13589 arg1Of -medium,35
R11328 T13591 T13592 arg1Of -medium,and
R11329 T13592 T13587 arg2Of and,at
R11330 T13594 T13595 arg1Of 35–55,%
R11331 T13596 T13592 arg2Of interface,and
R11332 T13596 T13593 arg1Of interface,the
R11333 T13596 T13594 arg1Of interface,35–55
R11334 T13598 T13599 arg1Of pooled,","
R11335 T13599 T13601 arg1Of ",",and
R11336 T13600 T13599 arg2Of washed,","
R11337 T13601 T13597 arg2Of and,were
R11338 T13602 T13601 arg2Of stained,and
R11339 T13602 T13603 arg1Of stained,with
R11340 T13605 T13603 arg2Of combination,with
R11341 T13605 T13604 arg1Of combination,a
R11342 T13605 T13606 arg1Of combination,of
R11343 T13605 T13609 modOf combination,to
R11344 T13605 T13628 modOf combination,were
R11345 T13608 T13606 arg2Of antibodies,of
R11346 T13608 T13607 arg1Of antibodies,various
R11347 T13610 T13609 arg1Of stain,to
R11348 T13613 T13610 arg2Of subsets,stain
R11349 T13613 T13611 arg1Of subsets,different
R11350 T13613 T13612 arg1Of subsets,DC
R11351 T13613 T13614 arg1Of subsets,","
R11352 T13616 T13617 arg1Of CD11c+CD8α+,","
R11353 T13617 T13619 arg1Of ",",and
R11354 T13618 T13617 arg2Of CD11c+CD8α−,","
R11355 T13619 T13614 arg2Of and,","
R11356 T13619 T13615 arg1Of and,conventional
R11357 T13621 T13619 arg2Of DC,and
R11358 T13621 T13620 arg1Of DC,plasmacytoid
R11359 T13623 T13622 arg2Of CD11c−PDCA-1+,(
R11360 T13624 T13622 arg3Of ),(
R11361 T13627 T13622 arg1Of panels,(
R11362 T13627 T13625 arg1Of panels,.The
R11363 T13627 T13626 arg1Of panels,top
R11364 T13627 T13628 arg1Of panels,were
R11365 T13627 T13629 arg2Of panels,gated
R11366 T13629 T13628 arg2Of gated,were
R11367 T13629 T13630 arg1Of gated,on
R11368 T13629 T13633 modOf gated,to
R11369 T13632 T13630 arg2Of cells,on
R11370 T13632 T13631 arg1Of cells,FSChiSSClo
R11371 T13634 T13633 arg1Of show,to
R11372 T13636 T13634 arg2Of populations,show
R11373 T13636 T13635 arg1Of populations,separate
R11374 T13636 T13637 arg1Of populations,of
R11375 T13638 T13639 arg1Of CD11c+PDCA-1−,and
R11376 T13640 T13639 arg2Of CD11c−PDCA-1+,and
R11377 T13641 T13637 arg2Of cells,of
R11378 T13641 T13638 arg1Of cells,CD11c+PDCA-1−
R11379 T13641 T13640 arg1Of cells,CD11c−PDCA-1+
R11380 T13643 T13642 arg1Of gating,Further
R11381 T13643 T13644 arg1Of gating,on
R11382 T13643 T13648 arg1Of gating,gave
R11383 T13647 T13644 arg2Of subpopulation,on
R11384 T13647 T13645 arg1Of subpopulation,the
R11385 T13647 T13646 arg1Of subpopulation,CD11c+PDCA-1−
R11386 T13651 T13648 arg2Of panel,gave
R11387 T13651 T13649 arg1Of panel,the
R11388 T13651 T13650 arg1Of panel,bottom
R11389 T13652 T13648 arg3Of that,gave
R11390 T13652 T13653 arg1Of that,showed
R11391 T13656 T13653 arg2Of pools,showed
R11392 T13656 T13654 arg1Of pools,two
R11393 T13656 T13655 arg1Of pools,distinct
R11394 T13656 T13657 arg1Of pools,of
R11395 T13661 T13657 arg2Of cells,of
R11396 T13661 T13658 arg1Of cells,CD11c+CD8α+
R11397 T13661 T13659 arg1Of cells,and
R11398 T13661 T13660 arg1Of cells,CD11c+CD8α−
R11399 T13662 T13663 arg1Of Percentages,were
R11400 T13662 T13664 arg2Of Percentages,calculated
R11401 T13662 T13671 arg1Of Percentages,were
R11402 T13662 T13673 arg2Of Percentages,shown
R11403 T13664 T13663 arg2Of calculated,were
R11404 T13664 T13665 arg1Of calculated,based
R11405 T13664 T13670 arg1Of calculated,and
R11406 T13666 T13665 arg2Of on,based
R11407 T13669 T13666 arg2Of population,on
R11408 T13669 T13667 arg1Of population,the
R11409 T13669 T13668 arg1Of population,parental
R11410 T13673 T13670 arg2Of shown,and
R11411 T13673 T13671 arg2Of shown,were
R11412 T13673 T13672 arg1Of shown,additionally
R11413 T13673 T13674 arg1Of shown,as
R11414 T13673 T13680 arg1Of shown,(
R11415 T13673 T13708 arg1Of shown,5
R11416 T13676 T13674 arg2Of graphs,as
R11417 T13676 T13675 arg1Of graphs,bar
R11418 T13676 T13677 arg1Of graphs,(
R11419 T13678 T13677 arg2Of n = 2,(
R11420 T13679 T13677 arg3Of ),(
R11421 T13681 T13680 arg2Of B,(
R11422 T13682 T13680 arg3Of ),(
R11423 T13684 T13683 arg2Of (,.
R11424 T13685 T13683 arg3Of C,.
R11425 T13686 T13683 arg1Of ) ,.
R11426 T13686 T13687 arg1Of ) ,WT
R11427 T13687 T13673 arg3Of WT ,shown
R11428 T13687 T13694 modOf WT ,cell
R11429 T13687 T13707 arg1Of WT ,2
R11430 T13689 T13687 arg2Of cpdm,WT
R11431 T13689 T13688 arg1Of cpdm,and
R11432 T13689 T13690 arg1Of cpdm,B
R11433 T13691 T13690 arg2Of MDC (,B
R11434 T13692 T13690 arg3Of 5,B
R11435 T13693 T13694 arg1Of ×105),cell
R11436 T13693 T13695 arg2Of ×105),s were sti
R11437 T13695 T13694 arg2Of s were sti,cell
R11438 T13695 T13696 arg1Of s were sti,mula
R11439 T13697 T13698 arg1Of ted wi,t
R11440 T13698 T13702 arg1Of t,00
R11441 T13699 T13700 arg1Of h m,edium
R11442 T13701 T13698 arg2Of ", 1",t
R11443 T13701 T13699 arg1Of ", 1",h m
R11444 T13702 T13696 arg2Of 00,mula
R11445 T13706 T13702 arg2Of r,00
R11446 T13706 T13703 arg1Of r,ng
R11447 T13706 T13704 arg1Of r,/ml L
R11448 T13706 T13705 arg1Of r,PS o
R11449 T13708 T13709 arg1Of 5,µg/
R11450 T13711 T13709 arg2Of poly ,µg/
R11451 T13711 T13710 arg1Of poly ,ml
R11452 T13713 T13712 arg1Of cells,The
R11453 T13713 T13714 arg1Of cells,were
R11454 T13713 T13715 arg2Of cells,labeled
R11455 T13713 T13726 arg2Of cells,subjected
R11456 T13713 T13728 arg1Of cells,flow
R11457 T13715 T13716 arg1Of labeled,with
R11458 T13715 T13725 arg1Of labeled,and
R11459 T13718 T13717 arg1Of anti-CD40,PE-labeled
R11460 T13718 T13719 arg1Of anti-CD40,","
R11461 T13719 T13722 arg1Of ",",and
R11462 T13720 T13719 arg2Of anti-CD80,","
R11463 T13722 T13716 arg2Of and,with
R11464 T13722 T13721 arg1Of and,","
R11465 T13723 T13722 arg2Of anti-CD86,and
R11466 T13725 T13714 arg2Of and,were
R11467 T13725 T13724 arg1Of and,","
R11468 T13726 T13725 arg2Of subjected,and
R11469 T13728 T13726 arg3Of flow,subjected
R11470 T13728 T13727 arg1Of flow,to
R11471 T13730 T13728 arg2Of analysis,flow
R11472 T13730 T13729 arg1Of analysis,cytometry
R11473 T13732 T13731 arg1Of populations,The
R11474 T13732 T13733 arg2Of populations,shown
R11475 T13732 T13736 arg1Of populations,were
R11476 T13732 T13737 arg2Of populations,gated
R11477 T13733 T13734 arg1Of shown,in
R11478 T13735 T13734 arg2Of histograms,in
R11479 T13737 T13736 arg2Of gated,were
R11480 T13737 T13738 arg1Of gated,on
R11481 T13740 T13738 arg2Of cells,on
R11482 T13740 T13739 arg1Of cells,CD11c+
R11483 T13742 T13741 arg1Of cells,Unstained
R11484 T13742 T13743 arg1Of cells,served
R11485 T13743 T13744 arg1Of served,as
R11486 T13746 T13744 arg2Of controls,as
R11487 T13746 T13745 arg1Of controls,negative
R11488 T13747 T13748 arg1Of Results,are
R11489 T13747 T13749 arg1Of Results,representative
R11490 T13749 T13748 arg2Of representative,are
R11491 T13749 T13750 arg1Of representative,of
R11492 T13753 T13750 arg2Of experiments,of
R11493 T13753 T13751 arg1Of experiments,two
R11494 T13753 T13752 arg1Of experiments,independent
R11703 T14019 T14018 arg1Of production,Defective
R11704 T14019 T14020 arg1Of production,of
R11705 T14022 T14020 arg2Of mediators,of
R11706 T14022 T14021 arg1Of mediators,pro-inflammatory
R11707 T14022 T14023 arg1Of mediators,from
R11708 T14026 T14023 arg2Of BMDC,from
R11709 T14026 T14024 arg2Of BMDC,stimulated
R11710 T14026 T14025 arg1Of BMDC,cpdm
R11711 T14028 T14027 arg2Of A–D,(
R11712 T14029 T14027 arg3Of ),(
R11713 T14031 T14032 arg1Of WT,and
R11714 T14033 T14032 arg2Of cpdm,and
R11715 T14034 T14027 arg1Of BMDC,(
R11716 T14034 T14030 arg1Of BMDC,Cultured
R11717 T14034 T14031 arg1Of BMDC,WT
R11718 T14034 T14033 arg1Of BMDC,cpdm
R11719 T14034 T14035 arg1Of BMDC,(
R11720 T14034 T14044 arg1Of BMDC,were
R11721 T14034 T14045 arg2Of BMDC,washed
R11722 T14034 T14047 arg2Of BMDC,stimulated
R11723 T14037 T14035 arg2Of cells,(
R11724 T14037 T14036 arg1Of cells,1×105
R11725 T14037 T14038 arg1Of cells,in
R11726 T14042 T14038 arg2Of medium,in
R11727 T14042 T14039 arg1Of medium,0.1
R11728 T14042 T14040 arg1Of medium,ml
R11729 T14042 T14041 arg1Of medium,complete
R11730 T14043 T14035 arg3Of ),(
R11731 T14045 T14046 arg1Of washed,and
R11732 T14046 T14044 arg2Of and,were
R11733 T14046 T14048 arg1Of and,with
R11734 T14047 T14046 arg2Of stimulated,and
R11735 T14049 T14050 arg1Of medium,","
R11736 T14050 T14054 arg1Of ",",or
R11737 T14051 T14052 arg1Of 100,ng/ml
R11738 T14053 T14050 arg2Of LPS,","
R11739 T14053 T14051 arg1Of LPS,100
R11740 T14054 T14048 arg2Of or,with
R11741 T14058 T14057 arg1Of I,poly
R11742 T14058 T14059 arg1Of I,:
R11743 T14060 T14054 arg2Of C,or
R11744 T14060 T14055 arg1Of C,25
R11745 T14060 T14056 arg1Of C,µg/ml
R11746 T14060 T14058 arg1Of C,I
R11747 T14060 T14061 arg1Of C,for
R11748 T14063 T14061 arg2Of hours,for
R11749 T14063 T14062 arg1Of hours,24
R11750 T14064 T14065 arg1Of Supernatants,were
R11751 T14064 T14066 arg2Of Supernatants,collected
R11752 T14066 T14065 arg2Of collected,were
R11753 T14066 T14067 arg1Of collected,for
R11754 T14068 T14067 arg2Of ELISA,for
R11755 T14068 T14069 arg1Of ELISA,of
R11756 T14068 T14078 arg1Of ELISA,for
R11757 T14069 T14077 arg1Of of,and
R11758 T14070 T14071 arg1Of IL12P70,","
R11759 T14071 T14074 arg1Of ",",and
R11760 T14072 T14071 arg2Of IL6,","
R11761 T14074 T14069 arg2Of and,of
R11762 T14074 T14073 arg1Of and,","
R11763 T14075 T14074 arg2Of GMCSF,and
R11764 T14077 T14076 arg1Of and,","
R11765 T14078 T14077 arg2Of for,and
R11766 T14079 T14078 arg2Of quantification,for
R11767 T14079 T14080 arg1Of quantification,of
R11768 T14082 T14080 arg2Of oxide,of
R11769 T14082 T14081 arg1Of oxide,nitric
R11770 T14082 T14083 arg1Of oxide,(
R11771 T14082 T14107 modOf oxide,dium
R11772 T14084 T14083 arg2Of NO,(
R11773 T14085 T14083 arg3Of ),(
R11774 T14087 T14086 arg2Of (E–,.
R11775 T14088 T14086 arg3Of F,.
R11776 T14090 T14086 arg1Of nt numb,.
R11777 T14090 T14089 arg1Of nt numb,) Gradie
R11778 T14090 T14091 arg1Of nt numb,e
R11779 T14090 T14105 arg1Of nt numb,et
R11780 T14090 T14107 arg1Of nt numb,dium
R11781 T14090 T14108 arg2Of nt numb,) of
R11782 T14092 T14093 arg1Of rs (1,×
R11783 T14093 T14096 arg1Of ×,×10
R11784 T14094 T14093 arg2Of "104, ",×
R11785 T14096 T14091 arg2Of ×10,e
R11786 T14096 T14095 arg1Of ×10,2
R11787 T14098 T14096 arg2Of d 4×1,×10
R11788 T14098 T14097 arg1Of d 4×1,"4, an"
R11789 T14098 T14099 arg1Of d 4×1,04
R11790 T14103 T14099 arg2Of L comp,04
R11791 T14103 T14100 arg1Of L comp,cel
R11792 T14103 T14101 arg1Of L comp,ls
R11793 T14103 T14102 arg1Of L comp,in 0.1 m
R11794 T14104 T14091 arg3Of l,e
R11795 T14106 T14105 arg2Of e me,et
R11796 T14108 T14107 arg2Of ) of,dium
R11797 T14112 T14108 arg3Of for100 ng/m,) of
R11798 T14112 T14109 arg1Of for100 ng/m,BMDC w
R11799 T14112 T14110 arg1Of for100 ng/m,ere u
R11800 T14112 T14111 arg1Of for100 ng/m,sed
R11801 T14114 T14113 arg2Of 24,After
R11802 T14115 T14126 modOf hours,were
R11803 T14115 T14134 arg2Of hours,rescued
R11804 T14115 T14138 arg1Of hours,had
R11805 T14118 T14117 arg1Of amounts,the
R11806 T14118 T14119 arg1Of amounts,of
R11807 T14118 T14123 arg1Of amounts,from
R11808 T14118 T14126 arg1Of amounts,were
R11809 T14118 T14127 arg2Of amounts,measured.
R11810 T14120 T14121 arg1Of IL12P70,and
R11811 T14121 T14119 arg2Of and,of
R11812 T14122 T14121 arg2Of IL6,and
R11813 T14125 T14123 arg2Of supernatant,from
R11814 T14125 T14124 arg1Of supernatant,the
R11815 T14127 T14116 arg1Of measured.,","
R11816 T14127 T14126 arg2Of measured.,were
R11817 T14127 T14128 arg1Of measured.,(
R11818 T14129 T14128 arg2Of G,(
R11819 T14130 T14128 arg3Of ),(
R11820 T14133 T14127 arg3Of mice,measured.
R11821 T14133 T14131 arg1Of mice,The
R11822 T14133 T14132 arg1Of mice,cpdm
R11823 T14137 T14134 arg1Of BAC,rescued
R11824 T14137 T14135 arg2Of BAC,by
R11825 T14137 T14136 arg1Of BAC,Sharpin-containing
R11826 T14138 T14113 arg1Of had,After
R11827 T14138 T14145 arg1Of had,[
R11828 T14140 T14138 arg2Of remission,had
R11829 T14140 T14139 arg1Of remission,complete
R11830 T14140 T14141 arg1Of remission,of
R11831 T14144 T14141 arg2Of phenotype,of
R11832 T14144 T14142 arg1Of phenotype,the
R11833 T14144 T14143 arg1Of phenotype,inflammatory
R11834 T14146 T14145 arg2Of 2,[
R11835 T14147 T14145 arg3Of ],[
R11836 T14148 T14149 arg1Of BMDC,developed
R11837 T14148 T14153 arg1Of BMDC,rescued
R11838 T14148 T14166 arg2Of BMDC,stimulated
R11839 T14149 T14150 arg1Of developed,from
R11840 T14149 T14152 arg1Of developed,and
R11841 T14151 T14150 arg2Of cpdm,from
R11842 T14153 T14161 arg1Of rescued,in
R11843 T14153 T14165 arg1Of rescued,and
R11844 T14154 T14156 modOf cpdm,were
R11845 T14155 T14156 arg1Of mice,were
R11846 T14155 T14157 arg2Of mice,plated
R11847 T14157 T14156 arg2Of plated,were
R11848 T14160 T14153 arg2Of cells,rescued
R11849 T14160 T14154 arg1Of cells,cpdm
R11850 T14160 T14158 arg1Of cells,(
R11851 T14160 T14159 arg1Of cells,1×106
R11852 T14163 T14161 arg2Of mL,in
R11853 T14163 T14162 arg1Of mL,0.3
R11854 T14163 T14164 arg1Of mL,)
R11855 T14165 T14152 arg2Of and,and
R11856 T14166 T14165 arg2Of stimulated,and
R11857 T14166 T14167 arg1Of stimulated,with
R11858 T14168 T14169 arg1Of 100,ng/mL
R11859 T14170 T14167 arg2Of LPS,with
R11860 T14170 T14168 arg1Of LPS,100
R11861 T14173 T14171 arg2Of hours,After
R11862 T14173 T14172 arg1Of hours,24
R11863 T14175 T14176 arg1Of supernatants,were
R11864 T14175 T14177 arg2Of supernatants,collected
R11865 T14177 T14171 arg1Of collected,After
R11866 T14177 T14174 arg1Of collected,","
R11867 T14177 T14176 arg2Of collected,were
R11868 T14177 T14178 arg1Of collected,for
R11869 T14179 T14178 arg2Of analysis,for
R11870 T14179 T14180 arg1Of analysis,of
R11871 T14182 T14180 arg2Of production,of
R11872 T14182 T14181 arg1Of production,IL12P70
R11873 T14183 T14184 arg1Of Data,are
R11874 T14183 T14185 arg1Of Data,representative
R11875 T14185 T14184 arg2Of representative,are
R11876 T14185 T14186 arg1Of representative,of
R11877 T14185 T14193 arg1Of representative,0.01
R11878 T14189 T14186 arg2Of experiments.,of
R11879 T14189 T14187 arg1Of experiments.,three
R11880 T14189 T14188 arg1Of experiments.,independent
R11881 T14193 T14190 arg1Of 0.01,*
R11882 T14193 T14191 arg1Of 0.01,P
R11883 T14193 T14192 arg1Of 0.01,<
R11884 T14193 T14194 arg1Of 0.01,;
R11885 T14198 T14194 arg2Of 0.005,;
R11886 T14198 T14195 arg1Of 0.005,**
R11887 T14198 T14196 arg1Of 0.005,P
R11888 T14198 T14197 arg1Of 0.005,<
R12030 T14390 T14388 arg2Of levels,Decreased
R12031 T14390 T14389 arg1Of levels,mRNA
R12032 T14390 T14391 arg1Of levels,of
R12033 T14393 T14391 arg2Of cytokines,of
R12034 T14393 T14392 arg1Of cytokines,inflammatory
R12035 T14393 T14394 arg1Of cytokines,from
R12036 T14396 T14394 arg2Of BMDC,from
R12037 T14396 T14395 arg1Of BMDC,cpdm
R12038 T14398 T14399 arg1Of WT,and
R12039 T14400 T14399 arg2Of cpdm,and
R12040 T14401 T14397 arg2Of BMDC,Cultured
R12041 T14401 T14398 arg1Of BMDC,WT
R12042 T14401 T14400 arg1Of BMDC,cpdm
R12043 T14401 T14402 arg1Of BMDC,(
R12044 T14401 T14411 arg1Of BMDC,were
R12045 T14401 T14412 arg2Of BMDC,washed
R12046 T14401 T14414 arg2Of BMDC,stimulated
R12047 T14404 T14402 arg2Of cells,(
R12048 T14404 T14403 arg1Of cells,5×105
R12049 T14404 T14405 arg1Of cells,in
R12050 T14409 T14405 arg2Of medium,in
R12051 T14409 T14406 arg1Of medium,0.2
R12052 T14409 T14407 arg1Of medium,ml
R12053 T14409 T14408 arg1Of medium,complete
R12054 T14410 T14402 arg3Of ),(
R12055 T14412 T14413 arg1Of washed,and
R12056 T14413 T14411 arg2Of and,were
R12057 T14413 T14415 arg1Of and,with
R12058 T14414 T14413 arg2Of stimulated,and
R12059 T14416 T14417 arg1Of 100,ng/ml
R12060 T14418 T14416 arg1Of LPS,100
R12061 T14418 T14419 arg1Of LPS,(
R12062 T14418 T14428 arg1Of LPS,or
R12063 T14420 T14421 arg1Of A,","
R12064 T14421 T14423 arg1Of ",",","
R12065 T14422 T14421 arg2Of C,","
R12066 T14423 T14419 arg2Of ",",(
R12067 T14423 T14425 arg1Of ",",","
R12068 T14424 T14423 arg2Of E,","
R12069 T14426 T14425 arg2Of G,","
R12070 T14427 T14419 arg3Of ),(
R12071 T14428 T14415 arg2Of or,with
R12072 T14434 T14428 arg2Of C,or
R12073 T14434 T14429 arg1Of C,25
R12074 T14434 T14430 arg1Of C,µg/ml
R12075 T14434 T14431 arg1Of C,poly
R12076 T14434 T14432 arg1Of C,I
R12077 T14434 T14433 arg1Of C,:
R12078 T14434 T14435 arg1Of C,(
R12079 T14436 T14435 arg2Of B,(
R12080 T14436 T14437 arg1Of B,","
R12081 T14436 T14441 arg1Of B,","
R12082 T14436 T14442 arg1Of B,H
R12083 T14438 T14439 arg1Of D,","
R12084 T14439 T14437 arg2Of ",",","
R12085 T14440 T14439 arg2Of F,","
R12086 T14443 T14435 arg3Of ),(
R12087 T14445 T14446 arg1Of 0,","
R12088 T14446 T14448 arg1Of ",",and
R12089 T14447 T14446 arg2Of 1,","
R12090 T14449 T14448 arg2Of 2,and
R12091 T14450 T14444 arg2Of hours,At
R12092 T14450 T14445 arg1Of hours,0
R12093 T14450 T14447 arg1Of hours,1
R12094 T14450 T14449 arg1Of hours,2
R12095 T14453 T14452 arg1Of RNA,total
R12096 T14453 T14454 arg1Of RNA,was
R12097 T14453 T14455 arg2Of RNA,extracted
R12098 T14453 T14457 arg1Of RNA,subject
R12099 T14455 T14456 arg1Of extracted,and
R12100 T14456 T14444 arg1Of and,At
R12101 T14456 T14451 arg1Of and,","
R12102 T14456 T14454 arg2Of and,was
R12103 T14457 T14456 arg2Of subject,and
R12104 T14457 T14458 arg1Of subject,to
R12105 T14459 T14458 arg2Of qRT-PCR,to
R12106 T14459 T14460 modOf qRT-PCR,to
R12107 T14461 T14460 arg1Of measure,to
R12108 T14463 T14464 arg1Of expression,of
R12109 T14463 T14478 arg1Of expression,","
R12110 T14465 T14466 arg1Of Il12p40,(
R12111 T14465 T14471 arg1Of Il12p40,","
R12112 T14467 T14466 arg2Of A,(
R12113 T14467 T14468 arg1Of A,","
R12114 T14469 T14468 arg2Of B,","
R12115 T14470 T14466 arg3Of ),(
R12116 T14471 T14464 arg2Of ",",of
R12117 T14471 T14473 arg1Of ",",(
R12118 T14472 T14471 arg2Of Il6,","
R12119 T14474 T14473 arg2Of C,(
R12120 T14474 T14475 arg1Of C,","
R12121 T14476 T14475 arg2Of D,","
R12122 T14477 T14473 arg3Of ),(
R12123 T14478 T14461 arg2Of ",",measure
R12124 T14478 T14462 arg1Of ",",the
R12125 T14479 T14480 arg1Of Gmcsf,(
R12126 T14479 T14486 arg1Of Gmcsf,and
R12127 T14481 T14480 arg2Of E,(
R12128 T14481 T14482 arg1Of E,","
R12129 T14483 T14482 arg2Of F,","
R12130 T14484 T14480 arg3Of ),(
R12131 T14486 T14478 arg2Of and,","
R12132 T14486 T14485 arg1Of and,","
R12133 T14487 T14488 arg1Of Ifnb,(
R12134 T14489 T14488 arg2Of G,(
R12135 T14489 T14490 arg1Of G,","
R12136 T14489 T14491 arg1Of G,H
R12137 T14492 T14488 arg3Of ),(
R12138 T14493 T14486 arg2Of mRNA,and
R12139 T14493 T14487 arg1Of mRNA,Ifnb
R12140 T14494 T14495 arg1Of Bars,represent
R12141 T14498 T14495 arg2Of SD,represent
R12142 T14498 T14496 arg1Of SD,mean
R12143 T14498 T14497 arg1Of SD,±
R12144 T14499 T14500 arg1Of Data,are
R12145 T14499 T14501 arg1Of Data,representative
R12146 T14501 T14500 arg2Of representative,are
R12147 T14501 T14502 arg1Of representative,of
R12148 T14505 T14502 arg2Of experiments,of
R12149 T14505 T14503 arg1Of experiments,two
R12150 T14505 T14504 arg1Of experiments,independent
R12302 T14699 T14698 arg1Of TLR4,Normal
R12303 T14699 T14700 arg1Of TLR4,and
R12304 T14700 T14704 arg1Of and,decreased
R12305 T14702 T14700 arg2Of expression,and
R12306 T14702 T14701 arg1Of expression,MD2
R12307 T14704 T14703 arg1Of decreased,and
R12308 T14706 T14705 arg1Of secretion,IL10
R12309 T14706 T14707 arg1Of secretion,and
R12310 T14707 T14704 arg2Of and,decreased
R12311 T14707 T14710 arg1Of and,by
R12312 T14709 T14707 arg2Of expression,and
R12313 T14709 T14708 arg1Of expression,A20
R12314 T14712 T14710 arg2Of BMDC,by
R12315 T14712 T14711 arg1Of BMDC,cpdm
R12316 T14714 T14713 arg2Of A,(
R12317 T14715 T14713 arg3Of ),(
R12318 T14717 T14713 arg1Of WT,(
R12319 T14717 T14716 arg1Of WT,Unstimulated
R12320 T14717 T14718 arg1Of WT,and
R12321 T14718 T14724 arg1Of and,were
R12322 T14718 T14725 arg2Of and,labeled
R12323 T14720 T14718 arg2Of BMDC,and
R12324 T14720 T14719 arg1Of BMDC,cpdm
R12325 T14720 T14721 arg1Of BMDC,(
R12326 T14722 T14721 arg2Of 5×105,(
R12327 T14723 T14721 arg3Of ),(
R12328 T14725 T14724 arg2Of labeled,were
R12329 T14725 T14726 arg1Of labeled,with
R12330 T14725 T14733 arg1Of labeled,subject
R12331 T14728 T14727 arg1Of anti-TLR4/MD2,PE-labeled
R12332 T14728 T14729 arg1Of anti-TLR4/MD2,or
R12333 T14729 T14726 arg2Of or,with
R12334 T14730 T14731 arg1Of anti-CD14,and
R12335 T14731 T14729 arg2Of and,or
R12336 T14732 T14731 arg2Of then,and
R12337 T14733 T14734 modOf subject,to
R12338 T14735 T14734 arg1Of flow,to
R12339 T14737 T14735 arg2Of analysis,flow
R12340 T14737 T14736 arg1Of analysis,cytometry
R12341 T14739 T14738 arg1Of cells,Unstained
R12342 T14739 T14740 arg1Of cells,serve
R12343 T14740 T14741 arg1Of serve,as
R12344 T14745 T14744 arg2Of B,(
R12345 T14746 T14744 arg3Of ),(
R12346 T14747 T14743 arg1Of Cultured,controls.
R12347 T14747 T14744 arg1Of Cultured,(
R12348 T14748 T14749 arg1Of WT,and
R12349 T14749 T14742 arg1Of and,negative
R12350 T14749 T14747 arg1Of and,Cultured
R12351 T14749 T14761 modOf and,were
R12352 T14749 T14776 arg1Of and,:
R12353 T14750 T14749 arg2Of cpdm,and
R12354 T14751 T14752 arg1Of BMDC,(
R12355 T14751 T14761 arg1Of BMDC,were
R12356 T14751 T14762 arg2Of BMDC,washed
R12357 T14751 T14764 arg2Of BMDC,stimulated
R12358 T14754 T14752 arg2Of cells,(
R12359 T14754 T14753 arg1Of cells,1×105
R12360 T14754 T14755 arg1Of cells,in
R12361 T14759 T14755 arg2Of medium,in
R12362 T14759 T14756 arg1Of medium,0.1
R12363 T14759 T14757 arg1Of medium,ml
R12364 T14759 T14758 arg1Of medium,complete
R12365 T14760 T14752 arg3Of ),(
R12366 T14762 T14763 arg1Of washed,and
R12367 T14763 T14761 arg2Of and,were
R12368 T14763 T14765 arg1Of and,with
R12369 T14764 T14763 arg2Of stimulated,and
R12370 T14766 T14767 arg1Of medium,","
R12371 T14767 T14771 arg1Of ",",or
R12372 T14768 T14769 arg1Of 100,ng/ml
R12373 T14770 T14767 arg2Of LPS,","
R12374 T14770 T14768 arg1Of LPS,100
R12375 T14771 T14765 arg2Of or,with
R12376 T14775 T14771 arg2Of I,or
R12377 T14775 T14772 arg1Of I,25
R12378 T14775 T14773 arg1Of I,µg/ml
R12379 T14775 T14774 arg1Of I,poly
R12380 T14777 T14741 arg2Of C,as
R12381 T14777 T14748 arg1Of C,WT
R12382 T14777 T14750 arg1Of C,cpdm
R12383 T14777 T14778 arg1Of C,for
R12384 T14780 T14778 arg2Of hours,for
R12385 T14780 T14779 arg1Of hours,24
R12386 T14781 T14782 arg1Of Supernatants,were
R12387 T14781 T14783 arg2Of Supernatants,collected
R12388 T14783 T14782 arg2Of collected,were
R12389 T14783 T14784 arg1Of collected,for
R12390 T14783 T14818 arg1Of collected,:
R12391 T14785 T14784 arg2Of ELISA,for
R12392 T14785 T14786 arg1Of ELISA,of
R12393 T14785 T14805 modOf ELISA,were
R12394 T14787 T14786 arg2Of IL10.,of
R12395 T14787 T14788 arg1Of IL10.,(
R12396 T14789 T14788 arg2Of C,(
R12397 T14790 T14788 arg3Of ),(
R12398 T14792 T14793 arg1Of WT,and
R12399 T14794 T14793 arg2Of cpdm,and
R12400 T14795 T14791 arg1Of BMDC,Cultured
R12401 T14795 T14792 arg1Of BMDC,WT
R12402 T14795 T14794 arg1Of BMDC,cpdm
R12403 T14795 T14796 arg1Of BMDC,(
R12404 T14795 T14805 arg1Of BMDC,were
R12405 T14795 T14806 arg2Of BMDC,washed
R12406 T14795 T14808 arg2Of BMDC,stimulated
R12407 T14798 T14796 arg2Of cells,(
R12408 T14798 T14797 arg1Of cells,5×105
R12409 T14798 T14799 arg1Of cells,in
R12410 T14803 T14799 arg2Of medium,in
R12411 T14803 T14800 arg1Of medium,0.2
R12412 T14803 T14801 arg1Of medium,ml
R12413 T14803 T14802 arg1Of medium,complete
R12414 T14804 T14796 arg3Of ),(
R12415 T14806 T14807 arg1Of washed,and
R12416 T14807 T14805 arg2Of and,were
R12417 T14807 T14809 arg1Of and,with
R12418 T14808 T14807 arg2Of stimulated,and
R12419 T14810 T14811 arg1Of 100,ng/ml
R12420 T14812 T14810 arg1Of LPS,100
R12421 T14812 T14813 arg1Of LPS,or
R12422 T14813 T14809 arg2Of or,with
R12423 T14816 T14813 arg2Of poly,or
R12424 T14816 T14814 arg1Of poly,25
R12425 T14816 T14815 arg1Of poly,µg/ml
R12426 T14816 T14817 arg1Of poly,I
R12427 T14819 T14838 arg2Of C.,measure
R12428 T14821 T14822 arg1Of 0,","
R12429 T14822 T14825 arg1Of ",",and
R12430 T14823 T14822 arg2Of 1,","
R12431 T14825 T14820 arg2Of and,At
R12432 T14825 T14824 arg1Of and,","
R12433 T14827 T14825 arg2Of hours,and
R12434 T14827 T14826 arg1Of hours,2
R12435 T14830 T14829 arg1Of RNA,total
R12436 T14830 T14831 arg1Of RNA,was
R12437 T14830 T14832 arg2Of RNA,extracted
R12438 T14830 T14834 arg1Of RNA,subject
R12439 T14832 T14833 arg1Of extracted,and
R12440 T14833 T14818 arg2Of and,:
R12441 T14833 T14820 arg1Of and,At
R12442 T14833 T14828 arg1Of and,","
R12443 T14833 T14831 arg2Of and,was
R12444 T14833 T14837 modOf and,to
R12445 T14834 T14833 arg2Of subject,and
R12446 T14834 T14835 arg1Of subject,to
R12447 T14836 T14835 arg2Of qRT-PCR,to
R12448 T14838 T14837 arg1Of measure,to
R12449 T14840 T14838 arg3Of production,measure
R12450 T14840 T14839 arg1Of production,the
R12451 T14840 T14841 arg1Of production,of
R12452 T14842 T14841 arg2Of A20,of
R12537 T14998 T14999 arg1Of Inhibition,of
R12538 T14998 T15002 arg1Of Inhibition,in
R12539 T15001 T14999 arg2Of signaling,of
R12540 T15001 T15000 arg1Of signaling,NF-κB
R12541 T15004 T15002 arg2Of BMDC,in
R12542 T15004 T15003 arg1Of BMDC,cpdm
R12543 T15005 T15006 arg1Of WT,and
R12544 T15007 T15006 arg2Of cpdm,and
R12545 T15008 T15005 arg1Of BMDC,WT
R12546 T15008 T15007 arg1Of BMDC,cpdm
R12547 T15008 T15009 arg1Of BMDC,(
R12548 T15008 T15018 arg1Of BMDC,were
R12549 T15008 T15019 arg2Of BMDC,stimulated
R12550 T15011 T15009 arg2Of cells,(
R12551 T15011 T15010 arg1Of cells,2×106
R12552 T15011 T15012 arg1Of cells,in
R12553 T15016 T15012 arg2Of medium,in
R12554 T15016 T15013 arg1Of medium,0.5
R12555 T15016 T15014 arg1Of medium,mL
R12556 T15016 T15015 arg1Of medium,complete
R12557 T15017 T15009 arg3Of ),(
R12558 T15019 T15018 arg2Of stimulated,were
R12559 T15019 T15020 arg1Of stimulated,with
R12560 T15021 T15022 arg1Of 100,ng/mL
R12561 T15023 T15021 arg1Of LPS,100
R12562 T15023 T15024 arg1Of LPS,(
R12563 T15023 T15027 arg1Of LPS,or
R12564 T15025 T15024 arg2Of A,(
R12565 T15026 T15024 arg3Of ),(
R12566 T15027 T15020 arg2Of or,with
R12567 T15033 T15027 arg2Of C,or
R12568 T15033 T15028 arg1Of C,25
R12569 T15033 T15029 arg1Of C,µg/mL
R12570 T15033 T15030 arg1Of C,poly
R12571 T15033 T15031 arg1Of C,I
R12572 T15033 T15032 arg1Of C,:
R12573 T15033 T15034 arg1Of C,(
R12574 T15035 T15034 arg2Of B,(
R12575 T15036 T15034 arg3Of ),(
R12576 T15038 T15039 arg1Of 0,","
R12577 T15039 T15041 arg1Of ",",","
R12578 T15040 T15039 arg2Of 15,","
R12579 T15041 T15044 arg1Of ",",and
R12580 T15042 T15041 arg2Of 30,","
R12581 T15044 T15037 arg2Of and,At
R12582 T15044 T15043 arg1Of and,","
R12583 T15046 T15044 arg2Of minutes,and
R12584 T15046 T15045 arg1Of minutes,60
R12585 T15049 T15048 arg1Of lysates,whole-cell
R12586 T15049 T15050 arg1Of lysates,were
R12587 T15049 T15051 arg2Of lysates,obtained
R12588 T15049 T15053 arg1Of lysates,subject
R12589 T15051 T15052 arg1Of obtained,and
R12590 T15052 T15037 arg1Of and,At
R12591 T15052 T15047 arg1Of and,","
R12592 T15052 T15050 arg2Of and,were
R12593 T15053 T15052 arg2Of subject,and
R12594 T15053 T15054 arg1Of subject,to
R12595 T15055 T15054 arg2Of immunoblots,to
R12596 T15055 T15056 arg1Of immunoblots,with
R12597 T15057 T15056 arg2Of antibodies,with
R12598 T15057 T15058 arg1Of antibodies,against
R12599 T15059 T15058 arg2Of proteins,against
R12600 T15059 T15060 arg2Of proteins,involved
R12601 T15060 T15061 arg1Of involved,in
R12602 T15062 T15063 arg1Of NF-κB,","
R12603 T15063 T15065 arg1Of ",",","
R12604 T15064 T15063 arg2Of TBK1/IRF3,","
R12605 T15065 T15068 arg1Of ",",and
R12606 T15066 T15065 arg2Of ERK1/2,","
R12607 T15068 T15067 arg1Of and,","
R12608 T15069 T15068 arg2Of p38,and
R12609 T15071 T15061 arg2Of pathways,in
R12610 T15071 T15062 arg1Of pathways,NF-κB
R12611 T15071 T15064 arg1Of pathways,TBK1/IRF3
R12612 T15071 T15066 arg1Of pathways,ERK1/2
R12613 T15071 T15069 arg1Of pathways,p38
R12614 T15071 T15070 arg1Of pathways,signaling
R12615 T15072 T15073 arg1Of Beta-actin,was
R12616 T15072 T15074 arg2Of Beta-actin,used
R12617 T15074 T15073 arg2Of used,was
R12618 T15074 T15075 arg1Of used,as
R12619 T15074 T15092 arg1Of used,:
R12620 T15076 T15075 arg2Of loading,as
R12621 T15079 T15078 arg2Of C,(
R12622 T15080 T15078 arg3Of ),(
R12623 T15082 T15076 arg2Of levels,loading
R12624 T15082 T15077 arg1Of levels,control.
R12625 T15082 T15078 arg1Of levels,(
R12626 T15082 T15081 arg1Of levels,Cellular
R12627 T15082 T15083 arg1Of levels,of
R12628 T15082 T15087 arg1Of levels,in
R12629 T15084 T15085 arg1Of p-IKK1/2,and
R12630 T15085 T15083 arg2Of and,of
R12631 T15086 T15085 arg2Of p-IκBα,and
R12632 T15088 T15089 arg1Of LPS-,or
R12633 T15089 T15087 arg2Of or,in
R12634 T15090 T15089 arg2Of poly,or
R12635 T15090 T15091 arg1Of poly,I
R12636 T15094 T15093 arg1Of BMDC,C-stimulated
R12637 T15094 T15095 arg1Of BMDC,were
R12638 T15094 T15096 arg2Of BMDC,quantitated
R12639 T15094 T15103 arg2Of BMDC,presented
R12640 T15096 T15097 arg1Of quantitated,with
R12641 T15096 T15102 arg1Of quantitated,and
R12642 T15098 T15097 arg2Of ImageJ,with
R12643 T15098 T15099 arg1Of ImageJ,(
R12644 T15100 T15099 arg2Of NIH,(
R12645 T15101 T15099 arg3Of ),(
R12646 T15102 T15092 arg2Of and,:
R12647 T15102 T15095 arg2Of and,were
R12648 T15103 T15102 arg2Of presented,and
R12649 T15103 T15104 arg1Of presented,as
R12650 T15106 T15104 arg2Of lines,as
R12651 T15106 T15105 arg1Of lines,trend
R12652 T15107 T15108 arg1Of Results,are
R12653 T15107 T15109 arg1Of Results,representative
R12654 T15109 T15108 arg2Of representative,are
R12655 T15109 T15110 arg1Of representative,of
R12656 T15113 T15111 arg1Of two,at
R12657 T15113 T15112 arg1Of two,least
R12658 T15115 T15110 arg2Of experiments,of
R12659 T15115 T15113 arg1Of experiments,two
R12660 T15115 T15114 arg1Of experiments,independent
R12806 T15343 T15341 arg2Of BMDC,Stimulated
R12807 T15343 T15342 arg1Of BMDC,cpdm
R12808 T15343 T15344 arg1Of BMDC,induced
R12809 T15347 T15344 arg2Of production,induced
R12810 T15347 T15345 arg1Of production,Th2-biased
R12811 T15347 T15346 arg1Of production,cytokine
R12812 T15347 T15348 arg1Of production,from
R12813 T15352 T15348 arg2Of cells,from
R12814 T15352 T15349 arg1Of cells,naïve
R12815 T15352 T15350 arg1Of cells,CD4+
R12816 T15352 T15351 arg1Of cells,T
R12817 T15353 T15354 arg1Of WT,and
R12818 T15355 T15354 arg2Of cpdm,and
R12819 T15356 T15353 arg1Of BMDC,WT
R12820 T15356 T15355 arg1Of BMDC,cpdm
R12821 T15356 T15357 arg1Of BMDC,(
R12822 T15356 T15371 arg1Of BMDC,were
R12823 T15356 T15372 arg2Of BMDC,incubated
R12824 T15359 T15357 arg2Of cells,(
R12825 T15359 T15358 arg1Of cells,5×104
R12826 T15359 T15360 arg1Of cells,in
R12827 T15359 T15368 arg1Of cells,:
R12828 T15364 T15361 arg1Of medium,0.1
R12829 T15364 T15362 arg1Of medium,mL
R12830 T15364 T15363 arg1Of medium,complete
R12831 T15364 T15365 arg1Of medium,;
R12832 T15365 T15360 arg2Of ;,in
R12833 T15367 T15365 arg2Of haplotype,;
R12834 T15367 T15366 arg1Of haplotype,MHC
R12835 T15369 T15368 arg2Of H-2b,:
R12836 T15370 T15357 arg3Of ),(
R12837 T15372 T15371 arg2Of incubated,were
R12838 T15372 T15373 arg1Of incubated,with
R12839 T15374 T15375 arg1Of medium,","
R12840 T15375 T15379 arg1Of ",",","
R12841 T15378 T15375 arg2Of LPS,","
R12842 T15378 T15376 arg1Of LPS,100
R12843 T15378 T15377 arg1Of LPS,ng/mL
R12844 T15379 T15386 arg1Of ",",or
R12845 T15383 T15382 arg1Of I,poly
R12846 T15383 T15384 arg1Of I,:
R12847 T15385 T15379 arg2Of C,","
R12848 T15385 T15380 arg1Of C,25
R12849 T15385 T15381 arg1Of C,µg/mL
R12850 T15385 T15383 arg1Of C,I
R12851 T15386 T15373 arg2Of or,with
R12852 T15389 T15386 arg2Of Pam3CYS,or
R12853 T15389 T15387 arg1Of Pam3CYS,5
R12854 T15389 T15388 arg1Of Pam3CYS,µg/mL
R12855 T15392 T15390 arg2Of hours,After
R12856 T15392 T15391 arg1Of hours,24
R12857 T15394 T15395 arg1Of cells,were
R12858 T15394 T15396 arg2Of cells,washed
R12859 T15394 T15400 arg2Of cells,incubated
R12860 T15396 T15397 arg1Of washed,with
R12861 T15396 T15399 arg1Of washed,and
R12862 T15398 T15397 arg2Of PBS,with
R12863 T15399 T15390 arg1Of and,After
R12864 T15399 T15393 arg1Of and,","
R12865 T15399 T15395 arg2Of and,were
R12866 T15400 T15399 arg2Of incubated,and
R12867 T15400 T15401 arg1Of incubated,with
R12868 T15403 T15402 arg1Of isolated,freshly
R12869 T15408 T15401 arg2Of cells,with
R12870 T15408 T15403 arg1Of cells,isolated
R12871 T15408 T15404 arg1Of cells,allogeneic
R12872 T15408 T15405 arg1Of cells,naïve
R12873 T15408 T15406 arg1Of cells,CD4+
R12874 T15408 T15407 arg1Of cells,T
R12875 T15408 T15409 arg1Of cells,(
R12876 T15411 T15409 arg2Of cells,(
R12877 T15411 T15410 arg1Of cells,2.5×105
R12878 T15411 T15412 arg1Of cells,in
R12879 T15411 T15420 arg1Of cells,:
R12880 T15416 T15413 arg1Of medium,0.1
R12881 T15416 T15414 arg1Of medium,mL
R12882 T15416 T15415 arg1Of medium,complete
R12883 T15416 T15417 arg1Of medium,;
R12884 T15417 T15412 arg2Of ;,in
R12885 T15419 T15417 arg2Of haplotype,;
R12886 T15419 T15418 arg1Of haplotype,MHC
R12887 T15421 T15420 arg2Of H-2d,:
R12888 T15422 T15409 arg3Of ),(
R12889 T15425 T15423 arg2Of days,After
R12890 T15425 T15424 arg1Of days,5
R12891 T15427 T15428 arg1Of supernatants,were
R12892 T15427 T15429 arg2Of supernatants,collected
R12893 T15429 T15428 arg2Of collected,were
R12894 T15429 T15430 arg1Of collected,and
R12895 T15430 T15423 arg1Of and,After
R12896 T15430 T15426 arg1Of and,","
R12897 T15432 T15431 arg1Of secretion,the
R12898 T15432 T15433 arg1Of secretion,of
R12899 T15432 T15440 arg1Of secretion,were
R12900 T15432 T15441 arg2Of secretion,measured
R12901 T15434 T15435 arg1Of IFNγ,","
R12902 T15435 T15438 arg1Of ",",and
R12903 T15436 T15435 arg2Of IL4,","
R12904 T15438 T15433 arg2Of and,of
R12905 T15438 T15437 arg1Of and,","
R12906 T15439 T15438 arg2Of IL2,and
R12907 T15441 T15430 arg2Of measured,and
R12908 T15441 T15440 arg2Of measured,were
R12909 T15443 T15441 arg1Of ELISA,measured
R12910 T15443 T15442 arg2Of ELISA,by
R12911 T15445 T15444 arg1Of controls,Negative
R12912 T15445 T15446 arg1Of controls,are
R12913 T15447 T15449 arg1Of 1,stimulated
R12914 T15449 T15446 arg2Of stimulated,are
R12915 T15449 T15448 arg1Of stimulated,)
R12916 T15449 T15451 arg1Of stimulated,without
R12917 T15450 T15449 arg2Of BMDC,stimulated
R12918 T15452 T15451 arg2Of co-culture,without
R12919 T15452 T15453 arg1Of co-culture,with
R12920 T15457 T15454 arg1Of cells,allogeneic
R12921 T15457 T15455 arg1Of cells,CD4+
R12922 T15457 T15456 arg1Of cells,T
R12923 T15457 T15458 arg1Of cells,;
R12924 T15458 T15453 arg2Of ;,with
R12925 T15459 T15460 arg1Of 2,)
R12926 T15464 T15458 arg2Of cells,;
R12927 T15464 T15459 arg1Of cells,2
R12928 T15464 T15461 arg1Of cells,allogeneic
R12929 T15464 T15462 arg1Of cells,CD4+
R12930 T15464 T15463 arg1Of cells,T
R12931 T15464 T15465 arg1Of cells,without
R12932 T15466 T15465 arg2Of co-culture,without
R12933 T15466 T15467 arg1Of co-culture,with
R12934 T15469 T15467 arg2Of BMDC,with
R12935 T15469 T15468 arg2Of BMDC,stimulated
R12936 T15470 T15471 arg1Of Samples,from
R12937 T15470 T15475 arg1Of Samples,had
R12938 T15474 T15471 arg2Of controls,from
R12939 T15474 T15472 arg1Of controls,both
R12940 T15474 T15473 arg1Of controls,negative
R12941 T15478 T15475 arg2Of production,had
R12942 T15478 T15476 arg1Of production,no
R12943 T15478 T15477 arg1Of production,detectable
R12944 T15478 T15479 arg1Of production,of
R12945 T15482 T15479 arg2Of cytokines,of
R12946 T15482 T15480 arg1Of cytokines,the
R12947 T15482 T15481 arg1Of cytokines,aforementioned
R12948 T15482 T15483 arg1Of cytokines,(
R12949 T15485 T15483 arg2Of shown,(
R12950 T15485 T15484 arg1Of shown,not
R12951 T15486 T15483 arg3Of ),(
R12952 T15487 T15488 arg1Of Results,are
R12953 T15487 T15489 arg2Of Results,analyzed
R12954 T15489 T15488 arg2Of analyzed,are
R12955 T15489 T15490 arg1Of analyzed,based
R12956 T15491 T15490 arg2Of on,based
R12957 T15493 T15491 arg2Of mice,on
R12958 T15493 T15492 arg1Of mice,2–4
R12959 T15493 T15494 arg1Of mice,per
R12960 T15495 T15494 arg2Of group.,per
R12961 T15499 T15489 arg3Of 0.05,analyzed
R12962 T15499 T15496 arg1Of 0.05,*
R12963 T15499 T15497 arg1Of 0.05,P
R12964 T15499 T15498 arg1Of 0.05,<
R2713 T3289 T3288 arg2Of cpdm,and
R2714 T3290 T3289 arg1Of mice,cpdm
R3321 T4014 T4021 arg1Of Production,is
R3322 T4014 T4022 arg2Of Production,impaired
R3327 T4020 T4019 arg1Of BMDC,cpdm
R3329 T4022 T4021 arg2Of impaired,is
R3330 T4023 T4024 arg1Of Incubation,with
R6555 T7953 T7949 arg2Of gene,of
R6556 T7953 T7950 arg1Of gene,the
R6557 T7953 T7951 arg1Of gene,mouse
R10395 T12508 T12512 arg1Of ",",or
R10396 T12509 T12510 arg1Of 100,ng/mL
R10397 T12511 T12509 arg1Of LPS,100
R10161 T12219 T12220 arg1Of Fibroblast,and
R10162 T12221 T12220 arg2Of macrophage,and
R10163 T12222 T12219 arg1Of cells,Fibroblast
R10164 T12222 T12221 arg1Of cells,macrophage
R10165 T12222 T12223 arg1Of cells,were
R10166 T12222 T12224 arg2Of cells,transfected
R10167 T12224 T12223 arg2Of transfected,were
R10168 T12224 T12225 arg1Of transfected,with
R10169 T12226 T12225 arg2Of pFLAG-SHARPIN,with
R7872 T9608 T9613 arg1Of carried,with
R7874 T9612 T9611 arg1Of accordance,strict
R7875 T9614 T9613 arg2Of protocols,with
R7876 T9614 T9615 arg2Of protocols,approved
R7877 T9620 T9618 arg1Of Care,Institutional
R7878 T9620 T9619 arg1Of Care,Animal
R7879 T9620 T9621 arg1Of Care,and
R7880 T9621 T9615 arg1Of and,approved
R7881 T9621 T9616 arg2Of and,by
R7882 T9621 T9617 arg1Of and,the
R7883 T9623 T9621 arg2Of Committees,and
R7884 T9623 T9622 arg1Of Committees,Use

bionlp-st-ge-2016-coref

Id Subject Object Predicate Lexical cue
T2686 5748-5757 Anaphor denotes its level
T2687 5695-5702 Antecedent denotes present
T1959 1885-1897 Anaphor denotes this protein
T1960 1771-1778 Antecedent denotes SHARPIN
T1961 2190-2194 Anaphor denotes mRNA
T1962 1997-2004 Antecedent denotes Sharpin
T1963 2220-2248 Anaphor denotes a functional protein product
T4194 10563-10582 Anaphor denotes all three cytokines
T4195 10451-10454 Antecedent denotes IL6
T4196 10469-10474 Antecedent denotes GMCSF
T15500 19241-19269 Anaphor denotes the aforementioned cytokines
T15501 18972-18976 Antecedent denotes IFNγ
T15502 18978-18981 Antecedent denotes IL4
T15503 18987-18990 Antecedent denotes IL2
T9037 20093-20098 Anaphor denotes which
T9038 20029-20036 Antecedent denotes SHARPIN
T9039 25078-25081 Anaphor denotes its
T9040 25024-25031 Antecedent denotes SHARPIN
T9041 25265-25291 Anaphor denotes the ubiquitylation process
T9042 25082-25120 Antecedent denotes linear-ubiquitin-chain-ligase activity
T3611 10183-10196 Anaphor denotes these markers
T3612 10092-10096 Antecedent denotes CD40
T3613 10098-10102 Antecedent denotes CD80
T3614 10108-10112 Antecedent denotes CD86
T11236 30187-30211 Anaphor denotes aforementioned cytokines
T11237 29939-29943 Antecedent denotes IFNγ
T11238 29945-29948 Antecedent denotes IL4
T11239 29950-29953 Antecedent denotes IL2
T11240 29959-29964 Antecedent denotes IL17A
R2262 T2686 T2687 boundBy its level,present
R1725 T1963 T1962 boundBy a functional protein product,Sharpin
R3501 T4194 T4195 boundBy all three cytokines,IL6
R3502 T4194 T4196 boundBy all three cytokines,GMCSF
R7641 T9037 T9038 boundBy which,SHARPIN
R7644 T9039 T9040 boundBy its,SHARPIN
R7645 T9041 T9042 boundBy the ubiquitylation process,linear-ubiquitin-chain-ligase activity
R1723 T1959 T1960 boundBy this protein,SHARPIN
R1724 T1961 T1962 boundBy mRNA,Sharpin
R12965 T15500 T15501 boundBy the aforementioned cytokines,IFNγ
R12966 T15500 T15502 boundBy the aforementioned cytokines,IL4
R12967 T15500 T15503 boundBy the aforementioned cytokines,IL2
R3047 T3611 T3612 boundBy these markers,CD40
R3048 T3611 T3613 boundBy these markers,CD80
R3049 T3611 T3614 boundBy these markers,CD86
R9352 T11236 T11237 boundBy aforementioned cytokines,IFNγ
R9353 T11236 T11238 boundBy aforementioned cytokines,IL4
R9354 T11236 T11239 boundBy aforementioned cytokines,IL2
R9355 T11236 T11240 boundBy aforementioned cytokines,IL17A

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T127 61-62 , denotes ,
T128 63-66 CC denotes and
T129 67-76 NNP denotes Induction
T130 77-79 IN denotes of
T131 80-83 NNP denotes Th1
T132 84-99 NNP denotes Differentiation
T133 100-102 IN denotes by
T134 103-112 NNP denotes Dendritic
T135 113-118 NNP denotes Cells
T136 165-176 NNP denotes Spontaneous
T137 177-186 NNS denotes mutations
T138 187-189 IN denotes of
T139 190-193 DT denotes the
T140 194-201 NNP denotes Sharpin
T141 202-203 -LRB- denotes (
T142 203-219 JJ denotes SHANK-associated
T143 220-222 NNP denotes RH
T144 223-241 NN denotes domain-interacting
T145 242-249 NN denotes protein
T146 249-250 , denotes ,
T147 251-256 JJ denotes other
T148 257-264 NNS denotes aliases
T149 264-265 : denotes :
T150 266-272 NNS denotes Rbckl1
T151 272-273 , denotes ,
T152 274-279 NNP denotes Sipl1
T153 279-280 -RRB- denotes )
T154 281-285 NN denotes gene
T155 286-288 IN denotes in
T156 289-293 NNS denotes mice
T157 294-300 VBP denotes result
T158 301-303 IN denotes in
T159 304-312 JJ denotes systemic
T160 313-325 NN denotes inflammation
T161 326-330 WDT denotes that
T162 331-333 VBZ denotes is
T163 334-347 VBN denotes characterized
T164 348-350 IN denotes by
T165 351-358 JJ denotes chronic
T166 359-372 JJ denotes proliferative
T167 373-383 NN denotes dermatitis
T168 384-387 CC denotes and
T169 388-400 VBD denotes dysregulated
T170 401-410 NN denotes secretion
T171 411-413 IN denotes of
T172 414-415 NNP denotes T
T173 416-423 CD denotes helper1
T174 424-425 -LRB- denotes (
T175 425-428 NN denotes Th1
T176 428-429 -RRB- denotes )
T177 430-433 CC denotes and
T178 434-437 JJ denotes Th2
T179 438-447 NNS denotes cytokines
T180 447-448 . denotes .
T181 449-452 DT denotes The
T182 453-461 JJ denotes cellular
T183 462-465 CC denotes and
T184 466-475 JJ denotes molecular
T185 476-486 NNS denotes mechanisms
T186 487-497 VBG denotes underlying
T187 498-502 DT denotes this
T188 503-515 JJ denotes inflammatory
T189 516-525 NN denotes phenotype
T190 526-532 VBP denotes remain
T191 533-540 JJ denotes elusive
T192 540-541 . denotes .
T193 542-551 JJ denotes Dendritic
T194 552-557 NNS denotes cells
T195 558-561 MD denotes may
T196 562-572 VB denotes contribute
T197 573-575 TO denotes to
T198 576-579 DT denotes the
T199 580-590 NN denotes initiation
T200 591-594 CC denotes and
T201 595-606 NN denotes progression
T202 607-609 IN denotes of
T203 610-613 DT denotes the
T204 614-623 NN denotes phenotype
T205 624-626 IN denotes of
T206 627-644 JJ denotes SHARPIN-deficient
T207 645-649 NNS denotes mice
T225 744-748 WDT denotes that
T208 650-657 IN denotes because
T209 658-660 IN denotes of
T210 661-666 PRP$ denotes their
T211 667-674 JJ denotes pivotal
T212 675-679 NN denotes role
T213 680-682 IN denotes in
T214 683-689 NN denotes innate
T215 690-693 CC denotes and
T216 694-702 JJ denotes adaptive
T217 703-711 NN denotes immunity
T218 711-712 . denotes .
T219 713-717 RB denotes Here
T220 718-720 PRP denotes we
T221 721-725 VBP denotes show
T222 726-728 IN denotes by
T223 729-733 NN denotes flow
T224 734-743 NN denotes cytometry
T226 749-756 NNP denotes SHARPIN
T227 756-757 : denotes -
T228 758-768 NN denotes deficiency
T229 769-772 VBD denotes did
T230 773-776 RB denotes not
T231 777-782 VB denotes alter
T232 783-786 DT denotes the
T233 787-799 NN denotes distribution
T234 800-802 IN denotes of
T235 803-812 JJ denotes different
T236 813-815 NN denotes DC
T237 816-824 NNS denotes subtypes
T238 825-827 IN denotes in
T239 828-831 DT denotes the
T240 832-838 NN denotes spleen
T241 838-839 . denotes .
T242 840-842 IN denotes In
T243 843-851 NN denotes response
T244 852-854 TO denotes to
T245 855-864 JJ denotes TOLL-like
T246 865-873 NN denotes receptor
T247 874-875 -LRB- denotes (
T248 875-878 NNP denotes TLR
T249 878-879 -RRB- denotes )
T250 880-888 VBZ denotes agonists
T251 889-892 NNP denotes LPS
T252 893-896 CC denotes and
T253 897-901 RB denotes poly
T254 902-903 PRP denotes I
T255 903-904 : denotes :
T256 904-905 NNP denotes C
T257 905-906 , denotes ,
T258 907-915 VBD denotes cultured
T259 916-920 NN denotes bone
T260 921-935 JJ denotes marrow-derived
T261 936-945 JJ denotes dendritic
T262 946-951 NNS denotes cells
T263 952-953 -LRB- denotes (
T264 953-957 NNP denotes BMDC
T265 957-958 -RRB- denotes )
T266 959-963 IN denotes from
T267 964-966 NNP denotes WT
T268 967-970 CC denotes and
T269 971-977 JJ denotes mutant
T270 978-982 NNS denotes mice
T271 983-992 VBD denotes exhibited
T272 993-1000 JJ denotes similar
T273 1001-1010 NNS denotes increases
T274 1011-1013 IN denotes in
T275 1014-1024 NN denotes expression
T276 1025-1027 IN denotes of
T277 1028-1042 JJ denotes co-stimulatory
T278 1043-1052 NNS denotes molecules
T279 1053-1057 NNP denotes CD40
T280 1057-1058 , denotes ,
T281 1059-1063 NNP denotes CD80
T282 1063-1064 , denotes ,
T283 1065-1068 CC denotes and
T284 1069-1073 NNP denotes CD86
T285 1073-1074 . denotes .
T286 1075-1082 RB denotes However
T287 1082-1083 , denotes ,
T288 1084-1094 VBD denotes stimulated
T289 1095-1112 JJ denotes SHARPIN-deficient
T290 1113-1117 NNP denotes BMDC
T291 1118-1121 VBD denotes had
T292 1122-1129 VBN denotes reduced
T293 1130-1143 NN denotes transcription
T294 1144-1147 CC denotes and
T295 1148-1157 NN denotes secretion
T296 1158-1160 IN denotes of
T297 1161-1177 JJ denotes pro-inflammatory
T298 1178-1187 NNS denotes mediators
T299 1188-1191 NNP denotes IL6
T300 1191-1192 , denotes ,
T301 1193-1200 NNP denotes IL12P70
T302 1200-1201 , denotes ,
T303 1202-1207 NNP denotes GMCSF
T304 1207-1208 , denotes ,
T305 1209-1212 CC denotes and
T306 1213-1219 JJ denotes nitric
T307 1220-1225 NN denotes oxide
T308 1225-1226 . denotes .
T309 1227-1233 JJ denotes Mutant
T310 1234-1238 NNP denotes BMDC
T311 1239-1242 VBD denotes had
T312 1243-1252 JJ denotes defective
T313 1253-1263 NN denotes activation
T314 1264-1266 IN denotes of
T315 1267-1272 JJ denotes NF-κB
T316 1273-1282 VBG denotes signaling
T317 1282-1283 , denotes ,
T318 1284-1291 IN denotes whereas
T319 1292-1295 DT denotes the
T320 1296-1303 NNP denotes MAPK1/3
T321 1304-1305 -LRB- denotes (
T322 1305-1311 NNP denotes ERK1/2
T323 1311-1312 -RRB- denotes )
T324 1313-1316 CC denotes and
T325 1317-1329 NNP denotes MAPK11/12/13
T326 1329-1330 NNP denotes /
T327 1330-1332 CD denotes 14
T328 1333-1334 -LRB- denotes (
T329 1334-1337 CD denotes p38
T330 1338-1341 NNP denotes MAP
T331 1342-1348 NN denotes kinase
T332 1349-1357 VBZ denotes isoforms
T333 1357-1358 -RRB- denotes )
T334 1359-1362 CC denotes and
T335 1363-1367 CD denotes TBK1
T336 1368-1377 VBG denotes signaling
T337 1378-1386 NNS denotes pathways
T338 1387-1391 VBD denotes were
T339 1392-1398 JJ denotes intact
T340 1398-1399 . denotes .
T341 1400-1401 DT denotes A
T342 1402-1407 JJ denotes mixed
T343 1408-1418 NN denotes lymphocyte
T344 1419-1427 NN denotes reaction
T345 1428-1434 VBD denotes showed
T346 1435-1439 IN denotes that
T347 1440-1446 JJ denotes mutant
T348 1447-1451 NNP denotes BMDC
T349 1452-1456 RB denotes only
T350 1457-1464 VBD denotes induced
T351 1465-1466 DT denotes a
T352 1467-1471 JJ denotes weak
T353 1472-1475 CD denotes Th1
T354 1476-1482 JJ denotes immune
T355 1483-1491 NN denotes response
T356 1492-1495 CC denotes but
T357 1496-1506 VBD denotes stimulated
T358 1507-1516 VBN denotes increased
T359 1517-1520 JJ denotes Th2
T360 1521-1529 NN denotes cytokine
T361 1530-1540 NN denotes production
T362 1541-1545 IN denotes from
T363 1546-1556 JJ denotes allogeneic
T364 1557-1562 NN denotes naïve
T365 1563-1566 NNP denotes CD4
T366 1566-1567 NNP denotes +
T367 1568-1569 NNP denotes T
T368 1570-1575 NNS denotes cells
T369 1575-1576 . denotes .
T370 1577-1579 IN denotes In
T371 1580-1590 NN denotes conclusion
T372 1590-1591 , denotes ,
T373 1592-1596 NN denotes loss
T374 1597-1599 IN denotes of
T375 1600-1607 NNP denotes Sharpin
T376 1608-1610 IN denotes in
T377 1611-1615 NNS denotes mice
T378 1616-1629 RB denotes significantly
T379 1630-1637 VBZ denotes affects
T380 1638-1641 DT denotes the
T381 1642-1648 JJ denotes immune
T382 1649-1657 NN denotes function
T383 1658-1660 IN denotes of
T384 1661-1663 NNP denotes DC
T385 1664-1667 CC denotes and
T386 1668-1672 DT denotes this
T387 1673-1676 MD denotes may
T388 1677-1686 RB denotes partially
T389 1687-1694 VB denotes account
T390 1695-1698 IN denotes for
T391 1699-1702 DT denotes the
T392 1703-1711 JJ denotes systemic
T393 1712-1724 NN denotes inflammation
T394 1725-1728 CC denotes and
T395 1729-1739 JJ denotes Th2-biased
T396 1740-1746 JJ denotes immune
T397 1747-1755 NN denotes response
T398 1755-1756 . denotes .
T1013 3940-3946 JJ denotes immune
T1014 3947-3956 NNS denotes responses
T1015 3957-3959 CC denotes or
T1016 3960-3969 NN denotes tolerance
T1017 3969-3970 . denotes .
T1018 3971-3973 NNP denotes DC
T1019 3974-3980 JJ denotes direct
T1020 3981-3984 DT denotes the
T1021 3985-4000 NN denotes differentiation
T1022 4001-4003 IN denotes of
T1023 4004-4007 CD denotes CD4
T1024 4007-4008 CD denotes +
T1025 4009-4010 NNP denotes T
T1026 4011-4016 NNS denotes cells
T1027 4016-4017 , denotes ,
T1028 4018-4021 CC denotes and
T1029 4022-4027 RB denotes hence
T1030 4028-4031 DT denotes the
T1031 4032-4036 NN denotes type
T1032 4037-4039 IN denotes of
T1033 4040-4046 JJ denotes immune
T1034 4047-4055 NN denotes response
T1035 4055-4056 , denotes ,
T1036 4057-4064 IN denotes through
T1037 4065-4068 DT denotes the
T1038 4069-4078 JJ denotes selective
T1039 4079-4088 NN denotes secretion
T1040 4089-4091 IN denotes of
T1041 4092-4101 NNS denotes cytokines
T1042 4101-4102 . denotes .
T1043 4103-4105 PRP denotes We
T1044 4106-4118 VBD denotes hypothesized
T1045 4119-4123 IN denotes that
T1046 4124-4133 JJ denotes defective
T1047 4134-4142 NN denotes cytokine
T1048 4143-4152 NN denotes secretion
T1049 4153-4155 IN denotes by
T1050 4156-4158 NNP denotes DC
T1051 4159-4170 VBD denotes contributed
T1052 4171-4173 TO denotes to
T1053 4174-4177 DT denotes the
T1054 4178-4188 JJ denotes Th2-biased
T1055 4189-4201 JJ denotes inflammatory
T1115 4580-4581 DT denotes a
T1116 4582-4587 JJ denotes novel
T1117 1771-1778 NNP denotes SHARPIN
T1118 1779-1782 VBD denotes was
T1119 1783-1793 RB denotes originally
T1120 1794-1804 VBN denotes identified
T1121 1805-1807 IN denotes in
T1122 1808-1821 JJ denotes post-synaptic
T1123 1822-1831 NNS denotes densities
T1124 1832-1834 IN denotes of
T1125 1835-1845 JJ denotes excitatory
T1126 1846-1854 NNS denotes synapses
T1127 1855-1857 IN denotes in
T1128 1858-1861 DT denotes the
T1129 1862-1867 NN denotes brain
T1130 1868-1870 IN denotes of
T1131 1871-1875 NNS denotes rats
T1132 1876-1877 VBP denotes [
T1133 1877-1878 CD denotes 1
T1134 1878-1879 NNP denotes ]
T1135 1879-1880 , denotes ,
T1136 1881-1884 CC denotes but
T1137 1885-1889 DT denotes this
T1138 1890-1897 NN denotes protein
T1139 1898-1900 VBZ denotes is
T1140 1901-1907 RB denotes widely
T1141 1908-1917 VBN denotes expressed
T1142 1918-1920 IN denotes in
T1143 1921-1922 DT denotes a
T1144 1923-1930 NN denotes variety
T1145 1931-1933 IN denotes of
T1146 1934-1941 NNS denotes tissues
T1147 1942-1943 NNP denotes [
T1148 1943-1944 CD denotes 2
T1149 1944-1945 NNP denotes ]
T1150 1945-1946 . denotes .
T1151 1947-1950 CD denotes Two
T1152 1951-1958 JJ denotes allelic
T1153 1958-1959 , denotes ,
T1154 1960-1969 JJ denotes autosomal
T1155 1970-1979 JJ denotes recessive
T1156 1980-1989 NNS denotes mutations
T1157 1990-1992 IN denotes in
T1158 1993-1996 DT denotes the
T1159 1997-2004 NNP denotes Sharpin
T1160 2005-2009 NN denotes gene
T1161 2010-2018 VBD denotes occurred
T1162 2019-2032 RB denotes spontaneously
T1163 2033-2035 IN denotes in
T1164 2036-2039 CD denotes two
T1165 2040-2046 JJ denotes inbred
T1166 2047-2054 NNS denotes strains
T1167 2055-2057 IN denotes of
T1168 2058-2062 NNS denotes mice
T1169 2062-2063 , denotes ,
T1170 2064-2101 NN denotes C57B/KaLawRij-Sharpincpdm/Sharpincpdm
T1171 2102-2105 CC denotes and
T1172 2106-2114 JJ denotes CBy.Ocb3
T1173 2114-2115 NN denotes /
T1174 2115-2150 NN denotes Dem-Sharpincpdm-Dem/Sharpincpdm-Dem
T1175 2150-2151 , denotes ,
T1176 2152-2161 VBG denotes resulting
T1177 2162-2164 IN denotes in
T1178 2165-2174 JJ denotes premature
T1179 2175-2186 NN denotes termination
T1180 2187-2189 IN denotes of
T1181 2190-2194 NNP denotes mRNA
T1182 2195-2204 NN denotes synthesis
T1183 2205-2208 CC denotes and
T1184 2209-2216 NN denotes absence
T1185 2217-2219 IN denotes of
T1186 2220-2221 DT denotes a
T1187 2222-2232 JJ denotes functional
T1188 2233-2240 NN denotes protein
T1189 2241-2248 NN denotes product
T1190 2249-2250 NNP denotes [
T1191 2250-2251 CD denotes 2
T1192 2251-2252 NNP denotes ]
T1193 2252-2253 . denotes .
T1194 2254-2261 IN denotes Despite
T1195 2262-2271 JJ denotes different
T1196 2272-2279 JJ denotes genetic
T1197 2280-2291 NNS denotes backgrounds
T1198 2291-2292 , denotes ,
T1199 2293-2297 DT denotes both
T1200 2298-2307 NNS denotes mutations
T1201 2308-2313 VBP denotes cause
T1202 2314-2321 JJ denotes similar
T1203 2322-2334 JJ denotes inflammatory
T1204 2335-2342 NN denotes disease
T1205 2343-2347 IN denotes with
T1206 2348-2354 JJ denotes severe
T1207 2355-2362 NN denotes chronic
T1208 2363-2374 JJ denotes progressive
T1209 2375-2385 NN denotes dermatitis
T1210 2386-2389 CC denotes and
T1211 2390-2399 JJ denotes defective
T1212 2400-2411 NN denotes development
T1213 2412-2414 IN denotes of
T1214 2415-2424 JJ denotes secondary
T1215 2425-2433 JJ denotes lymphoid
T1216 2434-2440 NNS denotes organs
T1217 2441-2442 VBP denotes [
T1218 2442-2443 CD denotes 2
T1219 2443-2444 JJ denotes ]
T1220 2445-2446 NN denotes [
T1221 2446-2447 CD denotes 4
T1222 2447-2448 NNP denotes ]
T1223 2448-2449 . denotes .
T1224 2450-2453 DT denotes The
T1225 2454-2464 NN denotes dermatitis
T1226 2465-2472 VBZ denotes becomes
T1227 2473-2483 RB denotes clinically
T1228 2484-2492 JJ denotes apparent
T1229 2493-2495 IN denotes at
T1230 2496-2501 IN denotes about
T1231 2502-2506 CD denotes four
T1232 2507-2512 NNS denotes weeks
T1233 2513-2515 IN denotes of
T1234 2516-2519 NN denotes age
T1235 2519-2520 . denotes .
T1236 2521-2526 EX denotes There
T1237 2527-2530 VBP denotes are
T1238 2531-2544 NNS denotes accumulations
T1239 2545-2547 IN denotes of
T1240 2548-2559 NNS denotes eosinophils
T1241 2559-2560 , denotes ,
T1242 2561-2572 NNS denotes neutrophils
T1243 2573-2576 CC denotes and
T1244 2577-2588 NNS denotes macrophages
T1245 2589-2591 IN denotes in
T1246 2592-2595 DT denotes the
T1247 2596-2600 NN denotes skin
T1248 2601-2603 IN denotes of
T1249 2604-2627 NNP denotes Sharpincpdm/Sharpincpdm
T1250 2628-2634 JJ denotes mutant
T1251 2635-2639 NNS denotes mice
T1252 2640-2641 -LRB- denotes (
T1253 2641-2650 NN denotes hereafter
T1254 2651-2659 VBD denotes referred
T1255 2660-2662 TO denotes to
T1256 2663-2665 IN denotes as
T1257 2666-2670 NN denotes cpdm
T1258 2671-2675 NNS denotes mice
T1259 2675-2676 -RRB- denotes )
T1260 2677-2687 VBN denotes associated
T1261 2688-2692 IN denotes with
T1262 2693-2702 VBN denotes increased
T1263 2703-2713 NN denotes expression
T1264 2714-2716 IN denotes of
T1265 2717-2720 JJ denotes Th2
T1266 2721-2730 NNS denotes cytokines
T1267 2731-2733 IN denotes in
T1268 2734-2737 DT denotes the
T1269 2738-2742 NN denotes skin
T1270 2743-2746 CC denotes and
T1271 2747-2749 IN denotes in
T1272 2750-2753 DT denotes the
T1273 2754-2766 NNS denotes supernatants
T1274 2767-2769 IN denotes of
T1275 2770-2779 VBN denotes activated
T1276 2780-2791 NNS denotes splenocytes
T1277 2792-2793 VBP denotes [
T1278 2793-2794 CD denotes 5
T1279 2794-2795 NNP denotes ]
T1280 2795-2796 . denotes .
T1281 2797-2800 DT denotes The
T1282 2801-2805 NNS denotes mice
T1283 2806-2810 VBP denotes have
T1284 2811-2813 DT denotes an
T1285 2814-2822 JJ denotes impaired
T1286 2823-2830 JJ denotes delayed
T1287 2831-2835 NN denotes type
T1288 2836-2852 NN denotes hypersensitivity
T1289 2853-2861 NN denotes response
T1290 2862-2865 CC denotes and
T1291 2866-2875 VBD denotes decreased
T1292 2876-2885 NN denotes secretion
T1293 2886-2888 IN denotes of
T1294 2889-2893 NNP denotes IFNγ
T1295 2894-2895 NNP denotes [
T1296 2895-2896 CD denotes 5
T1297 2896-2897 NNP denotes ]
T1298 2897-2898 , denotes ,
T1299 2899-2909 VBG denotes indicating
T1300 2910-2911 DT denotes a
T1301 2912-2918 NN denotes defect
T1302 2919-2921 IN denotes in
T1303 2922-2925 CD denotes Th1
T1304 2926-2932 JJ denotes immune
T1305 2933-2942 NNS denotes responses
T1306 2943-2946 CC denotes and
T1307 2947-2948 DT denotes a
T1308 2949-2953 NN denotes bias
T1309 2954-2961 IN denotes towards
T1310 2962-2963 DT denotes a
T1311 2964-2967 JJ denotes Th2
T1312 2968-2974 JJ denotes immune
T1313 2975-2983 NN denotes response
T1314 2983-2984 . denotes .
T1315 2985-2993 JJ denotes Systemic
T1316 2994-3003 NN denotes treatment
T1317 3004-3006 IN denotes of
T1318 3007-3011 NN denotes cpdm
T1319 3012-3016 NNS denotes mice
T1320 3017-3021 IN denotes with
T1321 3022-3033 JJ denotes recombinant
T1322 3034-3038 NNP denotes IL12
T1323 3039-3045 VBD denotes caused
T1324 3046-3054 JJ denotes complete
T1325 3055-3064 NN denotes remission
T1326 3065-3067 IN denotes of
T1327 3068-3071 DT denotes the
T1328 3072-3082 NN denotes dermatitis
T1329 3083-3084 NN denotes [
T1330 3084-3085 CD denotes 5
T1331 3085-3086 NNP denotes ]
T1332 3086-3087 . denotes .
T1333 3088-3102 NN denotes Neutralization
T1334 3103-3105 IN denotes of
T1335 3106-3109 CD denotes IL5
T1336 3110-3112 IN denotes by
T1337 3113-3121 NN denotes antibody
T1338 3122-3131 NN denotes treatment
T1339 3132-3134 CC denotes or
T1340 3135-3142 NNS denotes crosses
T1341 3143-3147 IN denotes with
T1342 3148-3161 JJ denotes IL5-deficient
T1343 3162-3166 NNS denotes mice
T1344 3167-3174 VBD denotes reduced
T1345 3175-3178 DT denotes the
T1346 3179-3185 NN denotes number
T1347 3186-3188 IN denotes of
T1348 3189-3200 VBG denotes circulating
T1349 3201-3204 CC denotes and
T1350 3205-3214 JJ denotes cutaneous
T1351 3215-3226 NNS denotes eosinophils
T1352 3226-3227 , denotes ,
T1353 3228-3231 CC denotes but
T1354 3232-3238 VBD denotes failed
T1355 3239-3241 TO denotes to
T1356 3242-3248 VB denotes reduce
T1357 3249-3252 DT denotes the
T1358 3253-3258 NN denotes onset
T1359 3259-3262 CC denotes and
T1360 3263-3271 NN denotes severity
T1361 3272-3274 IN denotes of
T1362 3275-3278 DT denotes the
T1363 3279-3289 NN denotes dermatitis
T1364 3290-3291 NN denotes [
T1365 3291-3292 CD denotes 6
T1366 3292-3293 NNP denotes ]
T1367 3293-3294 . denotes .
T1368 3295-3303 RB denotes Recently
T1369 3303-3304 , denotes ,
T1370 3305-3310 CD denotes three
T1371 3311-3322 JJ denotes independent
T1372 3323-3329 NNS denotes groups
T1373 3330-3340 VBN denotes identified
T1374 3341-3348 NNP denotes SHARPIN
T1375 3349-3351 IN denotes as
T1376 3352-3354 DT denotes an
T1377 3355-3364 JJ denotes essential
T1378 3365-3374 NN denotes component
T1379 3375-3377 IN denotes of
T1380 3378-3381 DT denotes the
T1381 3382-3388 JJ denotes linear
T1382 3389-3398 NN denotes ubiquitin
T1383 3399-3404 NN denotes chain
T1384 3405-3413 NN denotes assembly
T1385 3414-3421 NN denotes complex
T1386 3422-3423 -LRB- denotes (
T1387 3423-3428 NNP denotes LUBAC
T1388 3428-3429 -RRB- denotes )
T1389 3430-3434 WDT denotes that
T1390 3435-3444 VBZ denotes regulates
T1391 3445-3457 JJ denotes TNFα-induced
T1392 3458-3467 JJ denotes canonical
T1393 3468-3473 NN denotes NF-κB
T1394 3474-3483 VBG denotes signaling
T1395 3484-3485 NNP denotes [
T1396 3485-3486 CD denotes 7
T1397 3486-3487 NNP denotes ]
T1398 3488-3489 NN denotes [
T1399 3489-3490 CD denotes 9
T1400 3490-3491 NNP denotes ]
T1401 3491-3492 . denotes .
T1402 3493-3510 JJ denotes SHARPIN-deficient
T1403 3511-3516 NN denotes mouse
T1404 3517-3526 JJ denotes embryonic
T1405 3527-3537 NN denotes fibroblast
T1406 3538-3539 -LRB- denotes (
T1407 3539-3542 NNP denotes MEF
T1408 3542-3543 -RRB- denotes )
T1409 3544-3548 VBD denotes were
T1410 3549-3559 VBN denotes sensitized
T1411 3560-3562 TO denotes to
T1412 3563-3575 JJ denotes TNFα-induced
T1413 3576-3585 NN denotes apoptosis
T1414 3586-3589 CC denotes and
T1415 3590-3594 NN denotes cell
T1416 3595-3600 NN denotes death
T1417 3601-3604 VBD denotes was
T1418 3605-3615 VBN denotes implicated
T1419 3616-3618 IN denotes as
T1420 3619-3620 DT denotes a
T1421 3621-3627 NN denotes factor
T1422 3628-3630 IN denotes in
T1423 3631-3634 DT denotes the
T1424 3635-3645 NN denotes dermatitis
T1425 3646-3648 IN denotes of
T1426 3649-3653 NN denotes cpdm
T1427 3654-3658 NNS denotes mice
T1428 3659-3660 VBP denotes [
T1429 3660-3661 CD denotes 7
T1430 3661-3662 NNP denotes ]
T1431 3663-3664 NN denotes [
T1432 3664-3665 CD denotes 9
T1433 3665-3666 NNP denotes ]
T1434 3666-3667 . denotes .
T1435 3668-3677 JJ denotes Dendritic
T1436 3678-3683 NNS denotes cells
T1437 3684-3685 -LRB- denotes (
T1438 3685-3687 NNP denotes DC
T1439 3687-3688 -RRB- denotes )
T1440 3689-3693 VBP denotes have
T1441 3694-3695 DT denotes a
T1442 3696-3704 NN denotes sentinel
T1443 3705-3709 NN denotes role
T1444 3710-3712 IN denotes in
T1445 3713-3720 VBG denotes sensing
T1446 3721-3729 NN denotes pathogen
T1447 3730-3732 CC denotes or
T1448 3733-3739 NN denotes danger
T1449 3740-3747 NNS denotes signals
T1450 3748-3751 CC denotes and
T1451 3752-3760 NN denotes initiate
T1452 3761-3764 CC denotes and
T1453 3765-3771 JJ denotes direct
T1454 3772-3782 NN denotes activation
T1455 3783-3785 IN denotes of
T1456 3786-3789 DT denotes the
T1457 3790-3798 JJ denotes adaptive
T1458 3799-3805 JJ denotes immune
T1459 3806-3814 NN denotes response
T1460 3815-3816 NNP denotes [
T1461 3816-3818 CD denotes 10
T1462 3818-3819 NNP denotes ]
T1463 3819-3820 . denotes .
T1464 4588-4598 JJ denotes regulatory
T1465 4599-4607 NN denotes molecule
T1466 4608-4610 IN denotes in
T1467 4611-4613 NNP denotes DC
T1468 4614-4621 NN denotes biology
T1469 4622-4625 CC denotes and
T1470 4626-4633 VBP denotes suggest
T1471 4634-4638 IN denotes that
T1472 4639-4642 DT denotes the
T1473 4643-4655 JJ denotes dysregulated
T1474 4656-4664 NN denotes function
T1475 4665-4667 IN denotes of
T1476 4668-4685 JJ denotes SHARPIN-deficient
T1477 4686-4688 NNP denotes DC
T1478 4689-4694 VBZ denotes plays
T1479 4695-4696 DT denotes a
T1480 4697-4701 NN denotes role
T1481 4702-4704 IN denotes in
T1482 4705-4708 DT denotes the
T1483 4709-4713 NN denotes cpdm
T1484 4714-4723 NN denotes phenotype
T1485 4723-4724 . denotes .
T2226 4735-4751 NN denotes Characterization
T2227 4752-4754 IN denotes of
T2228 4755-4762 NNP denotes Sharpin
T2229 4764-4773 NNP denotes Orthologs
T2230 4774-4776 IN denotes of
T2231 4777-4784 NNP denotes SHARPIN
T2232 4785-4792 NN denotes protein
T2233 4793-4796 VBP denotes are
T2234 4797-4802 VBN denotes found
T2235 4803-4805 IN denotes in
T2236 4806-4813 JJ denotes various
T2237 4814-4821 NNS denotes species
T2238 4821-4822 , denotes ,
T2239 4823-4832 VBG denotes including
T2240 4833-4838 JJ denotes human
T2241 4838-4839 , denotes ,
T2242 4840-4845 NN denotes mouse
T2243 4846-4849 CC denotes and
T2244 4850-4853 NN denotes rat
T2245 4853-4854 . denotes .
T2246 4855-4860 NN denotes Motif
T2247 4861-4871 NN denotes prediction
T2248 4872-4883 NN denotes programming
T2249 4883-4884 , denotes ,
T2250 4885-4890 VBG denotes using
T2251 4891-4896 NNP denotes COILS
T2252 4897-4898 NNP denotes [
T2253 4898-4900 CD denotes 11
T2254 4900-4901 NNP denotes ]
T2255 4902-4905 CC denotes and
T2256 4906-4915 NNP denotes MotifScan
T2257 4916-4917 NNP denotes [
T2258 4917-4919 CD denotes 12
T2259 4919-4920 NNP denotes ]
T2260 4920-4921 , denotes ,
T2261 4922-4930 VBZ denotes suggests
T2262 4931-4935 IN denotes that
T2263 4936-4943 NNP denotes SHARPIN
T2264 4944-4952 VBZ denotes contains
T2265 4953-4954 DT denotes a
T2266 4955-4966 NN denotes coiled-coil
T2267 4967-4968 -LRB- denotes (
T2268 4968-4970 NNP denotes CC
T2269 4970-4971 -RRB- denotes )
T2270 4972-4978 NN denotes domain
T2271 4978-4979 , denotes ,
T2272 4980-4981 DT denotes a
T2273 4982-4996 JJ denotes ubiquitin-like
T2274 4997-4998 -LRB- denotes (
T2275 4998-5001 NNP denotes UBL
T2276 5001-5002 -RRB- denotes )
T2277 5003-5009 NN denotes domain
T2278 5009-5010 , denotes ,
T2279 5011-5014 CC denotes and
T2280 5015-5016 DT denotes a
T2281 5017-5028 NN denotes zinc-finger
T2282 5029-5040 NN denotes Ran-binding
T2283 5041-5048 NN denotes protein
T2284 5049-5050 CD denotes 2
T2285 5051-5052 -LRB- denotes (
T2286 5052-5057 NNP denotes ZFRBP
T2287 5057-5058 -RRB- denotes )
T2288 5059-5065 NN denotes domain
T2289 5065-5066 . denotes .
T2290 5067-5072 DT denotes These
T2291 5073-5083 JJ denotes functional
T2292 5084-5090 NNS denotes motifs
T2293 5091-5101 VBP denotes constitute
T2294 5102-5109 JJ denotes similar
T2295 5110-5116 NN denotes domain
T2296 5117-5125 NNS denotes profiles
T2297 5126-5130 WDT denotes that
T2298 5131-5134 VBP denotes are
T2299 5135-5142 JJ denotes present
T2300 5143-5145 IN denotes in
T2301 5146-5149 DT denotes the
T2302 5150-5157 NNP denotes SHARPIN
T2303 5158-5165 NN denotes protein
T2304 5166-5168 IN denotes of
T2305 5169-5172 DT denotes all
T2306 5173-5178 CD denotes three
T2307 5179-5186 NNS denotes origins
T2308 5187-5188 -LRB- denotes (
T2309 5188-5192 NNP denotes Fig.
T2310 5193-5195 NNP denotes 1A
T2311 5195-5196 -RRB- denotes )
T2312 5196-5197 , denotes ,
T2313 5198-5208 VBG denotes suggesting
T2314 5209-5213 IN denotes that
T2315 5214-5221 NNP denotes SHARPIN
T2316 5222-5228 VBZ denotes exerts
T2317 5229-5235 RB denotes highly
T2318 5236-5245 VBN denotes conserved
T2319 5246-5255 NNS denotes functions
T2320 5256-5262 IN denotes across
T2321 5263-5270 NNS denotes species
T2322 5270-5271 . denotes .
T2323 5272-5283 JJ denotes Spontaneous
T2324 5284-5293 NNS denotes mutations
T2325 5294-5296 IN denotes in
T2326 5297-5300 DT denotes the
T2327 5301-5306 NN denotes mouse
T2328 5307-5314 NNP denotes Sharpin
T2329 5315-5319 NN denotes gene
T2330 5320-5327 NNS denotes results
T2331 5328-5330 IN denotes in
T2332 5331-5332 DT denotes a
T2333 5333-5340 JJ denotes complex
T2334 5341-5353 JJ denotes inflammatory
T2335 5354-5363 NN denotes phenotype
T2336 5364-5377 VBN denotes characterized
T2337 5378-5380 IN denotes by
T2338 5381-5387 JJ denotes severe
T2339 5388-5398 NNS denotes dermatitis
T2340 5399-5400 -LRB- denotes (
T2341 5400-5404 NNP denotes Fig.
T2342 5405-5407 NNP denotes 1B
T2343 5407-5408 -RRB- denotes )
T2344 5408-5409 , denotes ,
T2345 5410-5418 JJ denotes systemic
T2346 5419-5431 NN denotes inflammation
T2347 5432-5435 CC denotes and
T2348 5436-5438 DT denotes an
T2349 5439-5447 JJ denotes enlarged
T2350 5448-5454 NN denotes spleen
T2351 5455-5456 -LRB- denotes (
T2352 5456-5460 NNP denotes Fig.
T2353 5461-5463 NNP denotes 1C
T2354 5463-5464 -RRB- denotes )
T2355 5465-5471 VBN denotes caused
T2356 5472-5474 IN denotes by
T2357 5475-5489 JJ denotes extramedullary
T2358 5490-5503 NN denotes hematopoiesis
T2359 5504-5505 NN denotes [
T2360 5505-5506 CD denotes 3
T2361 5506-5507 NNP denotes ]
T2362 5507-5508 . denotes .
T2363 5509-5512 DT denotes The
T2364 5513-5523 JJ denotes endogenous
T2365 5524-5534 NN denotes expression
T2366 5535-5537 IN denotes of
T2367 5538-5545 NNP denotes Sharpin
T2368 5546-5550 NNP denotes mRNA
T2369 5551-5553 IN denotes in
T2370 5554-5558 NNP denotes BMDC
T2371 5559-5562 VBD denotes was
T2372 5563-5573 VBN denotes determined
T2373 5574-5576 IN denotes by
T2374 5577-5589 JJ denotes quantitative
T2375 5590-5594 JJ denotes real
T2376 5595-5603 NN denotes time-PCR
T2377 5604-5605 -LRB- denotes (
T2378 5605-5612 NNP denotes qRT-PCR
T2379 5612-5613 -RRB- denotes )
T2380 5614-5623 JJ denotes following
T2381 5624-5631 NN denotes culture
T2382 5632-5634 IN denotes in
T2383 5635-5641 NN denotes medium
T2384 5642-5646 RB denotes only
T2385 5647-5649 CC denotes or
T2386 5650-5655 RB denotes after
T2387 5656-5667 NN denotes stimulation
T2388 5668-5672 IN denotes with
T2389 5673-5676 NNP denotes LPS
T2390 5676-5677 . denotes .
T2391 5678-5685 NNP denotes Sharpin
T2392 5686-5690 NNP denotes mRNA
T2393 5691-5694 VBD denotes was
T2394 5695-5702 JJ denotes present
T2395 5703-5705 IN denotes in
T2396 5706-5710 NNP denotes BMDC
T2397 5711-5720 VBD denotes generated
T2398 5721-5725 IN denotes from
T2399 5726-5728 NNP denotes WT
T2400 5729-5733 NNS denotes mice
T2401 5734-5735 -LRB- denotes (
T2402 5735-5739 NNP denotes Fig.
T2403 5740-5742 NNP denotes 1D
T2404 5742-5743 -RRB- denotes )
T2405 5744-5747 CC denotes and
T2406 5748-5751 PRP$ denotes its
T2407 5752-5757 NN denotes level
T2408 5758-5761 VBD denotes was
T2409 5762-5770 RB denotes modestly
T2410 5771-5780 VBN denotes decreased
T2411 5781-5783 IN denotes by
T2412 5784-5787 NNP denotes LPS
T2413 5788-5799 NN denotes stimulation
T2414 5799-5800 . denotes .
T2415 5801-5806 EX denotes There
T2416 5807-5810 VBD denotes was
T2417 5811-5812 DT denotes a
T2418 5813-5824 JJ denotes significant
T2419 5825-5834 NN denotes reduction
T2420 5835-5837 IN denotes of
T2421 5838-5845 NNP denotes Sharpin
T2422 5846-5850 NNP denotes mRNA
T2423 5851-5852 -LRB- denotes (
T2424 5852-5853 CD denotes 6
T2425 5854-5860 JJ denotes 7-fold
T2426 5860-5861 -RRB- denotes )
T2427 5862-5864 IN denotes in
T2428 5865-5869 NNP denotes BMDC
T2429 5870-5879 VBD denotes generated
T2430 5880-5884 IN denotes from
T2431 5885-5889 NN denotes cpdm
T2432 5890-5894 NNS denotes mice
T2433 5894-5895 . denotes .
T2434 5896-5908 NNP denotes Transfection
T2435 5909-5911 IN denotes of
T2436 5912-5923 NNP denotes Flag-tagged
T2437 5924-5931 NNP denotes Sharpin
T2438 5932-5934 IN denotes in
T2439 5935-5946 NNS denotes fibroblasts
T2440 5947-5948 -LRB- denotes (
T2441 5948-5954 CD denotes NIH3T3
T2442 5954-5955 -RRB- denotes )
T2443 5956-5959 CC denotes and
T2444 5960-5971 NNS denotes macrophages
T2445 5972-5973 -LRB- denotes (
T2446 5973-5979 CD denotes RAW264
T2447 5979-5981 CD denotes .7
T2448 5981-5982 -RRB- denotes )
T2449 5983-5992 VBD denotes indicated
T2450 5993-6004 JJ denotes cytoplasmic
T2451 6005-6017 NN denotes localization
T2452 6018-6020 IN denotes of
T2453 6021-6024 DT denotes the
T2454 6025-6032 NNP denotes SHARPIN
T2455 6033-6040 NN denotes protein
T2456 6041-6042 -LRB- denotes (
T2457 6042-6046 NNP denotes Fig.
T2458 6047-6049 NNP denotes 1E
T2459 6049-6050 -RRB- denotes )
T2460 6050-6051 . denotes .
T2885 7229-7240 VBG denotes Phenotyping
T2886 7241-7248 JJ denotes splenic
T2887 7249-7251 NNP denotes DC
T2888 7252-7255 CC denotes and
T2889 7256-7260 NNP denotes BMDC
T2890 7261-7265 IN denotes from
T2891 7266-7268 NNP denotes WT
T2892 7269-7272 CC denotes and
T2893 7273-7277 NN denotes cpdm
T2894 7278-7282 NNS denotes mice
T2895 7283-7285 NNP denotes DC
T2896 7286-7289 VBP denotes are
T2897 7290-7303 JJ denotes heterogeneous
T2898 7304-7307 CC denotes and
T2899 7308-7311 MD denotes can
T2900 7312-7314 VB denotes be
T2901 7315-7326 VBN denotes categorized
T2902 7327-7331 IN denotes into
T2903 7332-7340 JJ denotes multiple
T2904 7341-7349 NNS denotes subtypes
T2905 7350-7355 VBN denotes based
T2906 7356-7358 IN denotes on
T2907 7359-7366 NN denotes surface
T2908 7367-7374 NNS denotes markers
T2909 7375-7376 NNP denotes [
T2910 7376-7378 CD denotes 13
T2911 7378-7379 NNP denotes ]
T2912 7379-7380 . denotes .
T2913 7381-7383 TO denotes To
T2914 7384-7393 VB denotes determine
T2915 7394-7396 IN denotes if
T2916 7397-7400 DT denotes the
T2917 7401-7408 NNP denotes Sharpin
T2918 7409-7417 NN denotes mutation
T2919 7418-7425 VBZ denotes affects
T2920 7426-7428 NNP denotes DC
T2921 7429-7440 NN denotes development
T2922 7441-7443 IN denotes in
T2923 7444-7452 JJ denotes lymphoid
T2924 7453-7460 NNS denotes tissues
T2925 7460-7461 , denotes ,
T2926 7462-7467 NN denotes mouse
T2927 7468-7475 NNS denotes spleens
T2928 7476-7480 VBD denotes were
T2929 7481-7489 VBN denotes examined
T2930 7490-7493 IN denotes for
T2931 7494-7497 DT denotes the
T2932 7498-7510 NN denotes distribution
T2933 7511-7513 IN denotes of
T2934 7514-7526 JJ denotes conventional
T2935 7527-7529 NNP denotes DC
T2936 7530-7531 -LRB- denotes (
T2937 7531-7534 NNP denotes cDC
T2938 7534-7535 : denotes ;
T2939 7536-7541 NNP denotes CD11c
T2940 7541-7542 NNP denotes +
T2941 7542-7546 NNP denotes CD8α
T2942 7546-7547 NN denotes +
T2943 7548-7551 CC denotes and
T2944 7552-7557 JJ denotes CD11c
T2945 7557-7558 NN denotes +
T2946 7558-7562 NNP denotes CD8α
T2947 7562-7563 NNP denotes −
T2948 7563-7564 -RRB- denotes )
T2949 7565-7566 NNP denotes [
T2950 7566-7568 CD denotes 13
T2951 7568-7569 NNP denotes ]
T2952 7570-7573 CC denotes and
T2953 7574-7586 JJ denotes plasmacytoid
T2954 7587-7589 NNP denotes DC
T2955 7590-7591 -LRB- denotes (
T2956 7591-7594 NNP denotes pDC
T2957 7594-7595 : denotes ;
T2958 7596-7601 NNP denotes CD11c
T2959 7601-7602 NNP denotes −
T2960 7602-7608 NNP denotes PDCA-1
T2961 7608-7609 NNP denotes +
T2962 7609-7610 -RRB- denotes )
T2963 7611-7612 NNP denotes [
T2964 7612-7614 CD denotes 14
T2965 7614-7615 NNP denotes ]
T2966 7615-7616 . denotes .
T2967 7617-7620 DT denotes The
T2968 7621-7632 NNS denotes percentages
T2969 7633-7636 IN denotes for
T2970 7637-7644 JJ denotes splenic
T2971 7645-7648 NNP denotes cDC
T2972 7649-7652 CC denotes and
T2973 7653-7656 NNP denotes pDC
T2974 7657-7661 VBD denotes were
T2975 7662-7666 DT denotes both
T2976 7667-7674 VBN denotes reduced
T2977 7675-7677 IN denotes in
T2978 7678-7682 NN denotes cpdm
T2979 7683-7687 NNS denotes mice
T2980 7688-7692 WRB denotes when
T2981 7693-7701 VBN denotes compared
T2982 7702-7706 IN denotes with
T2983 7707-7709 NNP denotes WT
T2984 7710-7718 NNS denotes controls
T2985 7719-7720 -LRB- denotes (
T2986 7720-7724 NNP denotes Fig.
T2987 7725-7727 NNP denotes 2A
T2988 7727-7728 -RRB- denotes )
T2989 7728-7729 . denotes .
T2990 7730-7737 RB denotes However
T2991 7737-7738 , denotes ,
T2992 7739-7743 WRB denotes when
T2993 7744-7749 VBN denotes gated
T2994 7750-7752 IN denotes on
T2995 7753-7758 JJ denotes CD11c
T2996 7758-7759 NN denotes +
T2997 7760-7763 NNP denotes cDC
T2998 7763-7764 , denotes ,
T2999 7765-7768 DT denotes the
T3000 7769-7780 NNS denotes percentages
T3001 7781-7783 IN denotes of
T3002 7784-7788 JJ denotes CD8α
T3003 7788-7789 NN denotes +
T3004 7790-7793 CC denotes and
T3005 7794-7798 JJ denotes CD8α
T3006 7798-7799 NN denotes −
T3007 7800-7805 NNS denotes cells
T3008 7806-7810 VBD denotes were
T3009 7811-7814 RB denotes not
T3010 7815-7823 VBN denotes affected
T3011 7824-7826 IN denotes by
T3012 7827-7834 NNP denotes SHARPIN
T3013 7835-7845 NN denotes deficiency
T3014 7846-7847 -LRB- denotes (
T3015 7847-7851 NNP denotes Fig.
T3016 7852-7854 NNP denotes 2A
T3017 7854-7855 -RRB- denotes )
T3018 7855-7856 . denotes .
T3019 7857-7862 IN denotes Since
T3020 7863-7866 DT denotes the
T3021 7867-7873 NN denotes spleen
T3022 7874-7876 IN denotes of
T3023 7877-7881 NN denotes cpdm
T3024 7882-7886 NNS denotes mice
T3025 7887-7889 VBZ denotes is
T3026 7890-7898 RB denotes markedly
T3027 7899-7907 JJ denotes enlarged
T3028 7908-7911 CC denotes and
T3029 7912-7920 VBZ denotes contains
T3030 7921-7926 CD denotes three
T3031 7927-7932 NNS denotes times
T3032 7933-7935 IN denotes as
T3033 7936-7940 JJ denotes many
T3034 7941-7946 NNS denotes cells
T3035 7947-7948 -LRB- denotes (
T3036 7948-7952 NNP denotes Fig.
T3037 7953-7955 NNP denotes 1C
T3038 7955-7956 -RRB- denotes )
T3039 7956-7957 , denotes ,
T3040 7958-7961 DT denotes the
T3041 7962-7971 JJ denotes different
T3042 7972-7983 NNS denotes percentages
T3043 7984-7986 IN denotes of
T3044 7987-7994 JJ denotes splenic
T3045 7995-7998 NNP denotes cDC
T3046 7999-8002 CC denotes and
T3047 8003-8006 NNP denotes pDC
T3048 8007-8014 IN denotes between
T3049 8015-8017 NNP denotes WT
T3050 8018-8021 CC denotes and
T3051 8022-8028 JJ denotes mutant
T3052 8029-8033 NNS denotes mice
T3053 8034-8041 VBP denotes reflect
T3054 8042-8045 DT denotes the
T3055 8046-8055 VBN denotes increased
T3056 8056-8062 NN denotes number
T3057 8063-8065 IN denotes of
T3058 8066-8071 JJ denotes total
T3059 8072-8078 JJ denotes spleen
T3060 8079-8084 NNS denotes cells
T3061 8085-8091 RB denotes rather
T3062 8092-8096 IN denotes than
T3063 8097-8098 DT denotes a
T3064 8099-8108 NN denotes reduction
T3065 8109-8111 IN denotes in
T3066 8112-8115 NNP denotes cDC
T3067 8116-8119 CC denotes and
T3068 8120-8123 NNP denotes pDC
T3069 8124-8131 NNS denotes numbers
T3070 8131-8132 . denotes .
T3071 8133-8138 DT denotes These
T3072 8139-8143 NNS denotes data
T3073 8144-8152 VBP denotes indicate
T3074 8153-8157 IN denotes that
T3075 8158-8161 DT denotes the
T3076 8162-8169 NNP denotes Sharpin
T3077 8170-8178 NN denotes mutation
T3078 8179-8183 VBZ denotes does
T3079 8184-8187 RB denotes not
T3080 8188-8194 VB denotes affect
T3081 8195-8198 DT denotes the
T3082 8199-8211 NN denotes distribution
T3083 8212-8214 IN denotes of
T3084 8215-8218 DT denotes the
T3085 8219-8227 VBN denotes examined
T3086 8228-8230 NNP denotes DC
T3087 8231-8238 NNS denotes subsets
T3088 8239-8241 IN denotes in
T3089 8242-8245 DT denotes the
T3090 8246-8252 NN denotes spleen
T3091 8252-8253 . denotes .
T3092 9576-9580 NNP denotes BMDC
T3093 9581-9585 IN denotes from
T3094 9586-9588 IN denotes in
T3095 9589-9594 NN denotes vitro
T3096 9595-9603 NNS denotes cultures
T3097 9604-9616 RB denotes functionally
T3098 9617-9625 VBP denotes resemble
T3099 9626-9638 JJ denotes non-lymphoid
T3100 9639-9645 NN denotes tissue
T3101 9646-9648 NNP denotes DC
T3102 9649-9652 CC denotes and
T3103 9653-9669 JJ denotes monocyte-derived
T3104 9670-9682 JJ denotes inflammatory
T3105 9683-9685 NNP denotes DC
T3106 9686-9687 NNP denotes [
T3107 9687-9689 CD denotes 15
T3108 9689-9690 NNP denotes ]
T3109 9690-9691 , denotes ,
T3110 9692-9693 NNP denotes [
T3111 9693-9695 CD denotes 16
T3112 9695-9696 NNP denotes ]
T3113 9696-9697 . denotes .
T3114 9698-9701 DT denotes The
T3115 9702-9708 NNS denotes yields
T3116 9709-9711 IN denotes of
T3117 9712-9716 NNP denotes BMDC
T3118 9717-9721 IN denotes from
T3119 9722-9724 NNP denotes WT
T3120 9725-9728 CC denotes and
T3121 9729-9733 NN denotes cpdm
T3122 9734-9738 NNS denotes mice
T3123 9739-9743 VBD denotes were
T3124 9744-9751 JJ denotes similar
T3125 9751-9752 . denotes .
T3126 9753-9757 NNP denotes BMDC
T3127 9758-9762 VBD denotes were
T3128 9763-9768 JJ denotes CD11c
T3129 9768-9769 NN denotes +
T3130 9770-9773 CC denotes and
T3131 9774-9777 NNP denotes MHC
T3132 9778-9780 NNP denotes II
T3133 9780-9781 NN denotes +
T3134 9782-9786 IN denotes with
T3135 9787-9790 JJ denotes low
T3136 9791-9801 NN denotes expression
T3137 9802-9804 IN denotes of
T3138 9805-9819 JJ denotes co-stimulatory
T3139 9820-9829 NNS denotes molecules
T3140 9830-9834 NNP denotes CD40
T3141 9834-9835 , denotes ,
T3142 9836-9840 NNP denotes CD80
T3143 9840-9841 , denotes ,
T3144 9842-9845 CC denotes and
T3145 9846-9850 NNP denotes CD86
T3146 9850-9851 . denotes .
T3147 9852-9855 DT denotes The
T3148 9856-9860 CD denotes TLR3
T3149 9861-9867 NN denotes ligand
T3150 9868-9872 NN denotes poly
T3151 9873-9874 PRP denotes I
T3152 9874-9875 : denotes :
T3153 9875-9876 NNP denotes C
T3154 9877-9880 CC denotes and
T3155 9881-9884 DT denotes the
T3156 9885-9889 CD denotes TLR4
T3157 9890-9896 NN denotes ligand
T3158 9897-9900 NNP denotes LPS
T3159 9901-9905 DT denotes each
T3160 9906-9914 VBP denotes activate
T3161 9915-9926 VBG denotes overlapping
T3162 9927-9930 CC denotes but
T3163 9931-9940 JJ denotes different
T3164 9941-9950 VBG denotes signaling
T3165 9951-9959 NNS denotes pathways
T3166 9960-9963 CC denotes and
T3167 9964-9968 VBD denotes were
T3168 9969-9973 VBN denotes used
T3169 9974-9976 TO denotes to
T3170 9977-9983 VB denotes induce
T3171 9984-9986 NNP denotes DC
T3172 9987-9997 NN denotes maturation
T3173 9998-9999 NNP denotes [
T3174 9999-10001 CD denotes 17
T3175 10001-10002 NNP denotes ]
T3176 10002-10003 , denotes ,
T3177 10004-10005 NNP denotes [
T3178 10005-10007 CD denotes 18
T3179 10007-10008 NNP denotes ]
T3180 10008-10009 . denotes .
T3181 10010-10020 NNP denotes Incubation
T3182 10021-10025 IN denotes with
T3183 10026-10029 DT denotes the
T3184 10030-10033 NNP denotes TLR
T3185 10034-10042 NNS denotes agonists
T3186 10043-10046 IN denotes for
T3187 10047-10049 CD denotes 24
T3188 10050-10055 NNS denotes hours
T3189 10056-10064 VBD denotes resulted
T3190 10065-10067 IN denotes in
T3191 10068-10077 VBN denotes increased
T3192 10078-10088 NN denotes expression
T3193 10089-10091 IN denotes of
T3194 10092-10096 NNP denotes CD40
T3195 10096-10097 , denotes ,
T3196 10098-10102 NNP denotes CD80
T3197 10102-10103 , denotes ,
T3198 10104-10107 CC denotes and
T3199 10108-10112 NNP denotes CD86
T3200 10113-10115 IN denotes on
T3201 10116-10120 NNP denotes BMDC
T3202 10120-10121 : denotes ;
T3203 10122-10129 RB denotes however
T3204 10129-10130 , denotes ,
T3205 10131-10136 EX denotes there
T3206 10137-10140 VBD denotes was
T3207 10141-10143 DT denotes no
T3208 10144-10154 NN denotes difference
T3209 10155-10157 IN denotes in
T3210 10158-10161 DT denotes the
T3211 10162-10172 NN denotes expression
T3212 10173-10179 NNS denotes levels
T3213 10180-10182 IN denotes of
T3214 10183-10188 DT denotes these
T3215 10189-10196 NNS denotes markers
T3216 10197-10204 IN denotes between
T3217 10205-10207 NNP denotes WT
T3218 10208-10211 CC denotes and
T3219 10212-10216 VB denotes cpdm
T3220 10217-10221 NNP denotes BMDC
T3221 10222-10223 -LRB- denotes (
T3222 10223-10227 NNP denotes Fig.
T3223 10228-10230 NNP denotes 2B
T3224 10230-10231 -RRB- denotes )
T3225 10231-10232 . denotes .
T3226 10233-10237 RB denotes Thus
T3227 10237-10238 , denotes ,
T3228 10239-10246 NNP denotes SHARPIN
T3229 10247-10257 NN denotes deficiency
T3230 10258-10261 VBD denotes did
T3231 10262-10265 RB denotes not
T3232 10266-10275 VB denotes influence
T3233 10276-10279 DT denotes the
T3234 10280-10290 NN denotes expression
T3235 10291-10293 IN denotes of
T3236 10294-10308 JJ denotes co-stimulatory
T3237 10309-10318 NNS denotes molecules
T3238 10319-10321 IN denotes by
T3239 10322-10326 NNP denotes BMDC
T3240 10326-10327 . denotes .
T3828 10329-10339 NN denotes Production
T3829 10340-10342 IN denotes of
T3830 10343-10358 JJ denotes proinflammatory
T3831 10359-10368 NNS denotes mediators
T3832 10369-10371 IN denotes by
T3833 10372-10376 NN denotes cpdm
T3834 10377-10381 NNP denotes BMDC
T3835 10382-10384 VBZ denotes is
T3836 10385-10393 VBN denotes impaired
T3837 10394-10404 NNP denotes Incubation
T3838 10405-10409 IN denotes with
T3839 10410-10413 NNP denotes LPS
T3840 10414-10416 CC denotes or
T3841 10417-10421 RB denotes poly
T3842 10422-10423 PRP denotes I
T3843 10423-10424 : denotes :
T3844 10424-10425 NNP denotes C
T3845 10426-10434 VBD denotes resulted
T3846 10435-10437 IN denotes in
T3847 10438-10447 NN denotes secretion
T3848 10448-10450 IN denotes of
T3849 10451-10454 NNP denotes IL6
T3850 10454-10455 , denotes ,
T3851 10456-10463 NNP denotes IL12P70
T3852 10463-10464 , denotes ,
T3853 10465-10468 CC denotes and
T3854 10469-10474 NNP denotes GMCSF
T3855 10475-10479 IN denotes from
T3856 10480-10484 DT denotes both
T3857 10485-10487 NNP denotes WT
T3858 10488-10491 CC denotes and
T3859 10492-10496 VB denotes cpdm
T3860 10497-10501 NNP denotes BMDC
T3861 10501-10502 : denotes ;
T3862 10503-10510 RB denotes however
T3863 10510-10511 , denotes ,
T3864 10512-10516 NNP denotes BMDC
T3865 10517-10521 IN denotes from
T3866 10522-10526 NN denotes cpdm
T3867 10527-10531 NNS denotes mice
T3868 10532-10540 VBD denotes produced
T3869 10541-10554 RB denotes significantly
T3870 10555-10559 JJR denotes less
T3871 10560-10562 IN denotes of
T3872 10563-10566 DT denotes all
T3873 10567-10572 CD denotes three
T3874 10573-10582 NNS denotes cytokines
T3875 10583-10591 VBN denotes compared
T3876 10592-10596 IN denotes with
T3877 10597-10599 NNP denotes WT
T3878 10600-10604 NNP denotes BMDC
T3879 10605-10606 -LRB- denotes (
T3880 10606-10610 NNP denotes Fig.
T3881 10611-10613 NNP denotes 3A
T3882 10614-10615 NNP denotes C
T3883 10615-10616 , denotes ,
T3884 10617-10618 NNP denotes E
T3885 10619-10620 NN denotes F
T3886 10620-10621 -RRB- denotes )
T3887 10621-10622 . denotes .
T3888 10623-10626 DT denotes The
T3889 10627-10633 NN denotes amount
T3890 10634-10636 IN denotes of
T3891 10637-10643 JJ denotes nitric
T3892 10644-10649 NN denotes oxide
T3893 10650-10659 VBN denotes generated
T3894 10660-10662 IN denotes by
T3895 10663-10669 JJ denotes mutant
T3896 10670-10674 NNP denotes BMDC
T3897 10675-10678 VBD denotes was
T3898 10679-10683 RB denotes also
T3899 10684-10697 RB denotes significantly
T3900 10698-10705 VBN denotes reduced
T3901 10706-10714 VBN denotes compared
T3902 10715-10719 IN denotes with
T3903 10720-10722 NNP denotes WT
T3904 10723-10728 NNS denotes cells
T3905 10729-10730 -LRB- denotes (
T3906 10730-10734 NNP denotes Fig.
T3907 10735-10737 NNP denotes 3D
T3908 10737-10738 -RRB- denotes )
T3909 10738-10739 , denotes ,
T3910 10740-10750 VBG denotes indicating
T3911 10751-10759 RB denotes severely
T3912 10760-10769 VBN denotes disrupted
T3913 10770-10780 NN denotes production
T3914 10781-10783 IN denotes of
T3915 10784-10799 JJ denotes proinflammatory
T3916 10800-10809 NNS denotes mediators
T3917 10810-10814 IN denotes from
T3918 10815-10832 JJ denotes Sharpin-deficient
T3919 10833-10837 NNP denotes BMDC
T3920 10837-10838 . denotes .
T3921 10839-10841 IN denotes In
T3922 10842-10846 NN denotes vivo
T3923 10847-10862 NN denotes complementation
T3924 10863-10867 IN denotes with
T3925 10868-10869 DT denotes a
T3926 10870-10877 NNP denotes Sharpin
T3927 10878-10893 NN denotes gene-containing
T3928 10894-10897 NNP denotes BAC
T3929 10898-10906 VBD denotes reversed
T3930 10907-10910 DT denotes the
T3931 10911-10920 NN denotes phenotype
T3932 10921-10923 IN denotes of
T3933 10924-10927 DT denotes the
T3934 10928-10934 JJ denotes mutant
T3935 10935-10939 NNS denotes mice
T3936 10940-10941 VBP denotes [
T3937 10941-10942 CD denotes 2
T3938 10942-10943 NNP denotes ]
T3939 10943-10944 , denotes ,
T3940 10945-10948 CC denotes and
T3941 10949-10953 NNP denotes BMDC
T3942 10954-10963 VBD denotes generated
T3943 10964-10968 IN denotes from
T3944 10969-10974 DT denotes these
T3945 10975-10982 VBN denotes rescued
T3946 10983-10987 NNS denotes mice
T3947 10988-10996 VBD denotes secreted
T3948 10997-11010 RB denotes significantly
T3949 11011-11015 RBR denotes more
T3950 11016-11023 JJ denotes IL12P70
T3951 11024-11028 IN denotes than
T3952 11029-11033 NNP denotes BMDC
T3953 11034-11038 IN denotes from
T3954 11039-11043 NN denotes cpdm
T3955 11044-11048 NNS denotes mice
T3956 11049-11050 -LRB- denotes (
T3957 11050-11054 NNP denotes Fig.
T3958 11055-11057 NNP denotes 3G
T3959 11057-11058 -RRB- denotes )
T3960 11058-11059 , denotes ,
T3961 11060-11070 VBG denotes supporting
T3962 11071-11072 DT denotes a
T3963 11073-11082 JJ denotes necessary
T3964 11083-11087 NN denotes role
T3965 11088-11090 IN denotes of
T3966 11091-11098 NNP denotes SHARPIN
T3967 11099-11102 IN denotes for
T3968 11103-11106 DT denotes the
T3969 11107-11117 NN denotes production
T3970 11118-11120 IN denotes of
T3971 11121-11128 NNP denotes IL12P70
T3972 11128-11129 . denotes .
T3973 11130-11132 IN denotes In
T3974 11133-11141 NN denotes addition
T3975 11141-11142 , denotes ,
T3976 11143-11146 DT denotes the
T3977 11147-11157 NN denotes transcript
T3978 11158-11164 NNS denotes levels
T3979 11165-11167 IN denotes of
T3980 11168-11171 DT denotes the
T3981 11172-11184 JJ denotes inflammatory
T3982 11185-11194 NNS denotes cytokines
T3983 11195-11198 NNP denotes Il6
T3984 11198-11199 , denotes ,
T3985 11200-11207 NNP denotes Il12p40
T3986 11207-11208 , denotes ,
T3987 11209-11214 NNP denotes Gmcsf
T3988 11214-11215 , denotes ,
T3989 11216-11219 CC denotes and
T3990 11220-11224 NNP denotes Ifnb
T3991 11225-11229 VBD denotes were
T3992 11230-11238 VBN denotes examined
T3993 11238-11239 , denotes ,
T3994 11240-11243 CC denotes and
T3995 11244-11249 DT denotes these
T3996 11250-11254 VBD denotes were
T3997 11255-11258 DT denotes all
T3998 11259-11272 RB denotes significantly
T3999 11273-11280 VBN denotes reduced
T4000 11281-11283 IN denotes in
T4001 11284-11294 VBN denotes stimulated
T4002 11295-11299 NN denotes cpdm
T4003 11300-11304 NNP denotes BMDC
T4004 11305-11309 WRB denotes when
T4005 11310-11318 VBN denotes compared
T4006 11319-11323 IN denotes with
T4007 11324-11326 NNP denotes WT
T4008 11327-11335 NNS denotes controls
T4009 11336-11337 -LRB- denotes (
T4010 11337-11341 JJ denotes Fig.
T4011 11342-11343 CD denotes 4
T4012 11343-11344 -RRB- denotes )
T4013 11344-11345 . denotes .
T4489 12842-12850 VBN denotes Impaired
T4490 12851-12859 JJ denotes cytokine
T4491 12860-12870 NN denotes production
T4492 12871-12873 IN denotes by
T4493 12874-12878 NN denotes cpdm
T4494 12879-12883 NNP denotes BMDC
T4495 12884-12886 VBZ denotes is
T4496 12887-12897 VBN denotes correlated
T4497 12898-12902 IN denotes with
T4498 12903-12912 JJ denotes selective
T4499 12913-12920 NNS denotes defects
T4500 12921-12923 IN denotes in
T4501 12924-12929 JJ denotes NF-κB
T4502 12930-12939 VBG denotes signaling
T4503 12940-12945 EX denotes There
T4504 12946-12949 VBP denotes are
T4505 12950-12951 DT denotes a
T4506 12952-12958 NN denotes number
T4507 12959-12961 IN denotes of
T4508 12962-12970 JJ denotes possible
T4509 12971-12983 NNS denotes explanations
T4510 12984-12987 IN denotes for
T4511 12988-12991 DT denotes the
T4512 12992-13001 JJ denotes defective
T4513 13002-13010 NN denotes cytokine
T4514 13011-13020 NN denotes secretion
T4515 13021-13023 IN denotes in
T4516 13024-13034 VBN denotes stimulated
T4517 13035-13039 NN denotes cpdm
T4518 13040-13044 NNP denotes BMDC
T4519 13044-13045 , denotes ,
T4520 13046-13055 VBG denotes including
T4521 13056-13057 CD denotes 1
T4522 13057-13058 -RRB- denotes )
T4523 13059-13066 VBN denotes reduced
T4524 13067-13074 NN denotes surface
T4525 13075-13085 NN denotes expression
T4526 13086-13088 IN denotes of
T4527 13089-13092 DT denotes the
T4528 13093-13096 NNP denotes LPS
T4529 13097-13105 NN denotes receptor
T4530 13106-13113 NN denotes complex
T4531 13113-13114 , denotes ,
T4532 13115-13116 CD denotes 2
T4533 13116-13117 -RRB- denotes )
T4534 13118-13127 VBD denotes increased
T4535 13128-13138 NN denotes production
T4536 13139-13141 IN denotes of
T4537 13142-13159 JJ denotes anti-inflammatory
T4538 13160-13169 NNS denotes mediators
T4539 13169-13170 , denotes ,
T4540 13171-13172 LS denotes 3
T4541 13172-13173 -RRB- denotes )
T4542 13174-13183 VBD denotes increased
T4543 13184-13194 NN denotes expression
T4544 13195-13197 IN denotes of
T4545 13198-13206 JJ denotes negative
T4546 13207-13217 NNS denotes regulators
T4547 13218-13220 IN denotes of
T4548 13221-13224 NNP denotes TLR
T4549 13225-13233 NNS denotes pathways
T4550 13233-13234 , denotes ,
T4551 13235-13238 CC denotes and
T4552 13239-13240 LS denotes 4
T4553 13240-13241 -RRB- denotes )
T4554 13242-13250 VBN denotes impaired
T4555 13251-13262 JJ denotes TLR-induced
T4556 13263-13272 VBG denotes signaling
T4557 13273-13283 NN denotes activation
T4558 13283-13284 . denotes .
T4559 13285-13287 PRP denotes We
T4560 13288-13298 VBD denotes determined
T4561 13299-13302 DT denotes the
T4562 13303-13310 NN denotes surface
T4563 13311-13321 NN denotes expression
T4564 13322-13324 IN denotes of
T4565 13325-13328 DT denotes the
T4566 13329-13332 NNP denotes LPS
T4567 13333-13341 NN denotes receptor
T4568 13342-13349 NN denotes complex
T4569 13350-13354 WDT denotes that
T4570 13355-13364 VBZ denotes comprises
T4571 13365-13369 CD denotes TLR4
T4572 13369-13370 , denotes ,
T4573 13371-13374 DT denotes the
T4574 13375-13384 JJ denotes accessory
T4575 13385-13393 NNS denotes proteins
T4576 13394-13398 NNP denotes CD14
T4577 13399-13402 CC denotes and
T4578 13403-13410 JJ denotes myeloid
T4579 13411-13426 NN denotes differentiation
T4580 13427-13433 NN denotes factor
T4581 13434-13435 CD denotes 2
T4582 13436-13437 -LRB- denotes (
T4583 13437-13445 NNP denotes MD2/LY96
T4584 13445-13446 -RRB- denotes )
T4585 13447-13448 NNP denotes [
T4586 13448-13450 CD denotes 19
T4587 13450-13451 NNP denotes ]
T4588 13451-13452 . denotes .
T4589 13453-13457 NN denotes Flow
T4590 13458-13468 JJ denotes cytometric
T4591 13469-13477 NN denotes analysis
T4592 13478-13483 VBZ denotes shows
T4593 13484-13488 IN denotes that
T4594 13489-13492 DT denotes the
T4595 13493-13503 NN denotes expression
T4596 13504-13510 NNS denotes levels
T4597 13511-13513 IN denotes of
T4598 13514-13518 NNP denotes CD14
T4599 13519-13522 CC denotes and
T4600 13523-13531 NNP denotes TLR4/MD2
T4601 13532-13539 IN denotes between
T4602 13540-13542 NNP denotes WT
T4603 13543-13546 CC denotes and
T4604 13547-13551 VB denotes cpdm
T4605 13552-13556 NNP denotes BMDC
T4606 13557-13561 VBD denotes were
T4607 13562-13569 JJ denotes similar
T4608 13570-13571 -LRB- denotes (
T4609 13571-13575 NNP denotes Fig.
T4610 13576-13578 NNP denotes 5A
T4611 13578-13579 -RRB- denotes )
T4612 13579-13580 . denotes .
T4613 13581-13583 PRP denotes We
T4614 13584-13588 RB denotes then
T4615 13589-13599 VBD denotes quantified
T4616 13600-13603 DT denotes the
T4617 13604-13613 NN denotes secretion
T4618 13614-13616 IN denotes of
T4619 13617-13620 DT denotes the
T4620 13621-13632 JJ denotes suppressive
T4621 13633-13642 NNS denotes cytokines
T4622 13643-13647 VBP denotes IL10
T4623 13648-13652 IN denotes that
T4624 13653-13656 MD denotes can
T4625 13657-13664 VB denotes inhibit
T4626 13665-13669 NNP denotes IL12
T4627 13670-13679 NN denotes secretion
T4628 13680-13682 IN denotes in
T4629 13683-13685 DT denotes an
T4630 13686-13695 NN denotes autocrine
T4631 13696-13702 NN denotes manner
T4632 13703-13704 NNP denotes [
T4633 13704-13706 CD denotes 20
T4634 13706-13707 NNP denotes ]
T4635 13707-13708 , denotes ,
T4636 13709-13710 NNP denotes [
T4637 13710-13712 CD denotes 21
T4638 13712-13713 NNP denotes ]
T4639 13713-13714 . denotes .
T4640 13715-13718 DT denotes The
T4641 13719-13731 NNS denotes supernatants
T4642 13732-13736 IN denotes from
T4643 13737-13751 JJ denotes LPS-stimulated
T4644 13752-13756 NN denotes cpdm
T4645 13757-13761 NNP denotes BMDC
T4646 13762-13771 VBD denotes contained
T4647 13772-13785 RB denotes significantly
T4648 13786-13791 JJR denotes lower
T4649 13792-13798 NNS denotes levels
T4650 13799-13801 IN denotes of
T4651 13802-13806 NNP denotes IL10
T4652 13807-13811 IN denotes than
T4653 13812-13822 VBN denotes stimulated
T4654 13823-13825 NNP denotes WT
T4655 13826-13830 NNP denotes BMDC
T4656 13831-13832 -LRB- denotes (
T4657 13832-13836 NNP denotes Fig.
T4658 13837-13839 NNP denotes 5B
T4659 13839-13840 -RRB- denotes )
T4660 13840-13841 , denotes ,
T4661 13842-13852 VBG denotes suggesting
T4662 13853-13857 IN denotes that
T4663 13858-13862 NNP denotes IL10
T4664 13863-13866 VBD denotes was
T4665 13867-13870 RB denotes not
T4666 13871-13882 JJ denotes responsible
T4667 13883-13886 IN denotes for
T4668 13887-13896 VBN denotes decreased
T4669 13897-13906 NN denotes secretion
T4670 13907-13909 IN denotes of
T4671 13910-13917 CD denotes IL12P70
T4672 13918-13920 IN denotes by
T4673 13921-13925 NN denotes cpdm
T4674 13926-13930 NNP denotes BMDC
T4675 13930-13931 . denotes .
T4676 13932-13941 VBN denotes Increased
T4677 13942-13952 NN denotes expression
T4678 13953-13955 IN denotes of
T4679 13956-13957 DT denotes a
T4680 13958-13966 JJ denotes negative
T4681 13967-13976 NN denotes regulator
T4682 13977-13979 IN denotes of
T4683 13980-13983 NNP denotes TLR
T4684 13984-13993 VBG denotes signaling
T4685 13994-13998 JJ denotes such
T4686 13999-14001 IN denotes as
T4687 14002-14005 NNP denotes A20
T4688 14006-14007 NNP denotes [
T4689 14007-14009 CD denotes 22
T4690 14009-14010 NNP denotes ]
T4691 14011-14014 MD denotes may
T4692 14015-14019 RB denotes also
T4693 14020-14028 VB denotes suppress
T4694 14029-14037 JJ denotes cytokine
T4695 14038-14047 NN denotes secretion
T4696 14047-14048 . denotes .
T4697 14049-14056 RB denotes However
T4698 14056-14057 , denotes ,
T4699 14058-14061 DT denotes the
T4700 14062-14072 NN denotes transcript
T4701 14073-14078 NN denotes level
T4702 14079-14081 IN denotes of
T4703 14082-14085 NNP denotes A20
T4704 14086-14089 VBD denotes was
T4705 14090-14095 JJR denotes lower
T4706 14096-14098 IN denotes in
T4707 14099-14112 JJ denotes LPS-activated
T4708 14113-14117 NN denotes cpdm
T4709 14118-14122 NNP denotes BMDC
T4710 14123-14127 IN denotes than
T4711 14128-14130 NNP denotes WT
T4712 14131-14139 NNS denotes controls
T4713 14140-14141 -LRB- denotes (
T4714 14141-14145 NNP denotes Fig.
T4715 14146-14148 NNP denotes 5C
T4716 14148-14149 -RRB- denotes )
T4717 14149-14150 , denotes ,
T4718 14151-14158 RB denotes thereby
T4719 14159-14165 VBG denotes ruling
T4720 14166-14169 RP denotes out
T4721 14170-14184 NN denotes overexpression
T4722 14185-14187 IN denotes of
T4723 14188-14191 NNP denotes A20
T4724 14192-14194 IN denotes as
T4725 14195-14196 DT denotes a
T4726 14197-14203 NN denotes factor
T4727 14204-14206 IN denotes in
T4728 14207-14210 DT denotes the
T4729 14211-14218 VBN denotes reduced
T4730 14219-14227 NN denotes cytokine
T4731 14228-14238 NN denotes production
T4732 14238-14239 . denotes .
T4733 15014-15017 DT denotes The
T4734 15018-15031 NN denotes transcription
T4735 15032-15034 IN denotes of
T4736 15035-15049 JJ denotes TLR3/4-induced
T4737 15050-15065 JJ denotes proinflammatory
T4738 15066-15079 NNS denotes intermediates
T4739 15080-15082 VBZ denotes is
T4740 15083-15090 RB denotes tightly
T4741 15091-15100 VBN denotes regulated
T4742 15101-15103 IN denotes by
T4743 15104-15112 JJ denotes cellular
T4744 15113-15122 VBG denotes signaling
T4745 15123-15131 NNS denotes pathways
T4746 15131-15132 , denotes ,
T4747 15133-15135 IN denotes in
T4748 15136-15146 JJ denotes particular
T4749 15147-15152 NN denotes NF-κB
T4750 15152-15153 , denotes ,
T4751 15154-15163 CD denotes TBK1/IRF3
T4752 15163-15164 , denotes ,
T4753 15165-15168 CC denotes and
T4754 15169-15173 NNP denotes MAPK
T4755 15174-15175 NNP denotes [
T4756 15175-15177 CD denotes 23
T4757 15177-15178 NNP denotes ]
T4758 15179-15180 NNP denotes [
T4759 15180-15182 CD denotes 27
T4760 15182-15183 NNP denotes ]
T4761 15183-15184 . denotes .
T4762 15185-15187 PRP denotes We
T4763 15188-15192 JJ denotes next
T4764 15193-15203 VBN denotes determined
T4765 15204-15206 IN denotes if
T4766 15207-15216 VBN denotes disrupted
T4767 15217-15222 NNP denotes NF-κB
T4768 15222-15223 , denotes ,
T4769 15224-15233 CD denotes TBK1/IRF3
T4770 15233-15234 , denotes ,
T4771 15235-15241 CC denotes and/or
T4772 15242-15246 NNP denotes MAPK
T4773 15247-15256 VBG denotes signaling
T4774 15257-15260 MD denotes may
T4775 15261-15269 VB denotes underlie
T4776 15270-15273 DT denotes the
T4777 15274-15282 VBN denotes impaired
T4778 15283-15291 NN denotes cytokine
T4779 15292-15302 NN denotes production
T4780 15303-15307 IN denotes from
T4781 15308-15318 VBN denotes stimulated
T4782 15319-15336 JJ denotes Sharpin-deficient
T4783 15337-15341 NNP denotes BMDC
T4784 15341-15342 . denotes .
T4785 15343-15359 JJ denotes Stimulus-induced
T4786 15360-15375 NN denotes phosphorylation
T4787 15376-15378 IN denotes of
T4788 15379-15382 DT denotes the
T4789 15383-15386 NNP denotes IκB
T4790 15387-15393 NN denotes kinase
T4791 15394-15395 -LRB- denotes (
T4792 15395-15401 NNP denotes IKK1/2
T4793 15401-15402 -RRB- denotes )
T4794 15403-15405 VBZ denotes is
T4795 15406-15408 DT denotes an
T4796 15409-15418 JJ denotes essential
T4797 15419-15423 NN denotes step
T4798 15424-15426 IN denotes in
T4799 15427-15432 JJ denotes NF-κB
T4800 15433-15442 VBG denotes signaling
T4801 15442-15443 , denotes ,
T4802 15444-15452 VBG denotes allowing
T4803 15453-15468 NN denotes phosphorylation
T4804 15469-15472 CC denotes and
T4805 15473-15492 JJ denotes proteasome-mediated
T4806 15493-15504 NN denotes degradation
T4807 15505-15507 IN denotes of
T4808 15508-15511 DT denotes the
T4809 15512-15517 NNP denotes NF-κB
T4810 15518-15527 NN denotes inhibitor
T4811 15528-15532 NNP denotes IκBα
T4812 15533-15535 TO denotes to
T4813 15536-15543 VB denotes release
T4814 15544-15547 DT denotes the
T4815 15548-15553 JJ denotes NF-κB
T4816 15554-15567 NN denotes transcription
T4817 15568-15575 NNS denotes factors
T4818 15576-15580 IN denotes into
T4819 15581-15584 DT denotes the
T4820 15585-15592 NN denotes nucleus
T4821 15592-15593 . denotes .
T4822 15594-15597 DT denotes The
T4823 15598-15604 NN denotes amount
T4824 15605-15607 IN denotes of
T4825 15608-15622 VBN denotes phosphorylated
T4826 15623-15629 NNP denotes IKK1/2
T4827 15630-15631 -LRB- denotes (
T4828 15631-15637 JJ denotes p-IKK1
T4829 15637-15638 NN denotes /
T4830 15638-15639 CD denotes 2
T4831 15639-15640 -RRB- denotes )
T4832 15641-15644 CC denotes and
T4833 15645-15649 NNP denotes IκBα
T4834 15650-15651 -LRB- denotes (
T4835 15651-15657 NNP denotes p-IκBα
T4836 15657-15658 -RRB- denotes )
T4837 15659-15668 VBG denotes following
T4838 15669-15679 NN denotes incubation
T4839 15680-15684 IN denotes with
T4840 15685-15688 NNP denotes LPS
T4841 15689-15691 CC denotes or
T4842 15692-15696 RB denotes poly
T4843 15697-15698 PRP denotes I
T4844 15698-15699 : denotes :
T4845 15699-15700 NNP denotes C
T4846 15701-15704 VBD denotes was
T4847 15705-15713 RB denotes severely
T4848 15714-15723 VBN denotes decreased
T4849 15724-15726 IN denotes in
T4850 15727-15731 NN denotes cpdm
T4851 15732-15736 NNP denotes BMDC
T4852 15737-15739 IN denotes as
T4853 15740-15748 VBN denotes compared
T4854 15749-15753 IN denotes with
T4855 15754-15756 NNP denotes WT
T4856 15757-15765 NNS denotes controls
T4857 15766-15767 -LRB- denotes (
T4858 15767-15771 NN denotes Fig.
T4859 15772-15773 CD denotes 6
T4860 15773-15774 -RRB- denotes )
T4861 15774-15775 . denotes .
T4862 15776-15779 DT denotes The
T4863 15780-15784 NN denotes cpdm
T4864 15785-15789 NNP denotes BMDC
T4865 15790-15799 VBD denotes exhibited
T4866 15800-15807 JJ denotes similar
T4867 15808-15814 NNS denotes levels
T4868 15815-15817 IN denotes of
T4869 15818-15822 CD denotes TBK1
T4870 15822-15823 , denotes ,
T4871 15824-15830 NNP denotes ERK1/2
T4872 15830-15831 , denotes ,
T4873 15832-15835 CC denotes and
T4874 15836-15839 CD denotes p38
T4875 15840-15855 NN denotes phosphorylation
T4876 15856-15858 TO denotes to
T4877 15859-15864 DT denotes those
T4878 15865-15867 IN denotes of
T4879 15868-15870 NNP denotes WT
T4880 15871-15876 NNS denotes cells
T4881 15877-15878 -LRB- denotes (
T4882 15878-15882 NN denotes Fig.
T4883 15883-15884 CD denotes 6
T4884 15884-15885 -RRB- denotes )
T4885 15885-15886 . denotes .
T4886 15887-15892 DT denotes These
T4887 15893-15900 NNS denotes results
T4888 15901-15909 VBP denotes indicate
T4889 15910-15914 IN denotes that
T4890 15915-15918 DT denotes the
T4891 15919-15926 NN denotes absence
T4892 15927-15929 IN denotes of
T4893 15930-15940 JJ denotes functional
T4894 15941-15948 NNP denotes SHARPIN
T4895 15949-15958 VBD denotes decreased
T4896 15959-15964 NNP denotes NF-κB
T4897 15965-15975 NN denotes activation
T4898 15976-15979 CC denotes but
T4899 15980-15983 VBD denotes did
T4900 15984-15987 RB denotes not
T4901 15988-15994 VB denotes affect
T4902 15995-16004 CD denotes TBK1/IRF3
T4903 16004-16005 , denotes ,
T4904 16006-16012 NNP denotes ERK1/2
T4905 16012-16013 , denotes ,
T4906 16014-16017 CC denotes and
T4907 16018-16021 CD denotes p38
T4908 16022-16031 VBG denotes signaling
T4909 16032-16034 IN denotes in
T4910 16035-16039 NNP denotes BMDC
T4911 16039-16040 . denotes .
T5830 16693-16703 JJ denotes Th2-biased
T5831 16704-16718 NN denotes immunogenicity
T5832 16719-16721 IN denotes of
T5833 16722-16732 VBN denotes stimulated
T5834 16733-16737 NN denotes cpdm
T5835 16738-16742 NNP denotes BMDC
T5836 16743-16746 NNP denotes The
T5837 16747-16756 JJ denotes defective
T5838 16757-16761 NN denotes IL12
T5839 16762-16772 NN denotes production
T5840 16773-16774 -LRB- denotes (
T5841 16774-16778 NNP denotes Fig.
T5842 16779-16781 NNP denotes 3A
T5843 16781-16782 -RRB- denotes )
T5844 16783-16786 CC denotes and
T5845 16787-16799 JJ denotes Th2-dominant
T5846 16800-16808 NN denotes cytokine
T5847 16809-16816 NN denotes profile
T5848 16817-16819 IN denotes in
T5849 16820-16824 NN denotes cpdm
T5850 16825-16829 NNS denotes mice
T5851 16830-16831 VBP denotes [
T5852 16831-16832 CD denotes 5
T5853 16832-16833 JJ denotes ]
T5854 16834-16841 VBP denotes suggest
T5855 16842-16846 IN denotes that
T5856 16847-16850 DT denotes the
T5857 16851-16858 NN denotes absence
T5858 16859-16861 IN denotes of
T5859 16862-16869 NNP denotes SHARPIN
T5860 16870-16877 VBZ denotes affects
T5861 16878-16881 DT denotes the
T5862 16882-16889 NN denotes ability
T5863 16890-16892 IN denotes of
T5864 16893-16897 NN denotes cpdm
T5865 16898-16902 NNP denotes BMDC
T5866 16903-16905 TO denotes to
T5867 16906-16912 VB denotes induce
T5868 16913-16914 NNP denotes T
T5869 16915-16919 NN denotes cell
T5870 16920-16935 NN denotes differentiation
T5871 16936-16940 IN denotes into
T5872 16941-16949 NN denotes effector
T5873 16950-16955 NNS denotes cells
T5874 16955-16956 . denotes .
T5875 16957-16967 NN denotes Co-culture
T5876 16968-16970 IN denotes of
T5877 16971-16981 JJ denotes allogeneic
T5878 16982-16987 NN denotes naïve
T5879 16988-16991 NNP denotes CD4
T5880 16991-16992 NNP denotes +
T5881 16993-16994 NNP denotes T
T5882 16995-17000 NNS denotes cells
T5883 17001-17005 IN denotes with
T5884 17006-17008 NNP denotes WT
T5885 17009-17013 NNP denotes BMDC
T5886 17014-17024 VBD denotes stimulated
T5887 17025-17029 IN denotes with
T5888 17030-17033 NNP denotes LPS
T5889 17034-17036 CC denotes or
T5890 17037-17041 RB denotes poly
T5891 17042-17043 PRP denotes I
T5892 17043-17044 : denotes :
T5893 17044-17045 NNP denotes C
T5894 17046-17054 VBD denotes elicited
T5895 17055-17061 JJ denotes robust
T5896 17062-17066 JJ denotes IFNγ
T5897 17067-17077 NN denotes production
T5898 17077-17078 , denotes ,
T5899 17079-17086 IN denotes whereas
T5900 17087-17090 DT denotes the
T5901 17091-17104 NN denotes concentration
T5902 17105-17107 IN denotes of
T5903 17108-17112 NNP denotes IFNγ
T5904 17113-17115 IN denotes in
T5905 17116-17120 NN denotes cpdm
T5906 17121-17127 NNP denotes BMDC-T
T5907 17128-17132 NN denotes cell
T5908 17133-17141 NNS denotes cultures
T5909 17142-17145 VBD denotes was
T5910 17146-17159 RB denotes significantly
T5911 17160-17165 JJR denotes lower
T5912 17166-17171 IN denotes after
T5913 17172-17175 NNP denotes LPS
T5914 17176-17187 NN denotes stimulation
T5915 17188-17189 -LRB- denotes (
T5916 17189-17193 NNP denotes Fig.
T5917 17194-17196 NN denotes 7A
T5918 17196-17197 -RRB- denotes )
T5919 17197-17198 , denotes ,
T5920 17199-17209 VBG denotes indicating
T5921 17210-17218 VBN denotes impaired
T5922 17219-17233 JJ denotes Th1-polarizing
T5923 17234-17243 NNS denotes abilities
T5924 17244-17246 IN denotes of
T5925 17247-17251 NN denotes cpdm
T5926 17252-17256 NNP denotes BMDC
T5927 17256-17257 . denotes .
T5928 17258-17260 IN denotes In
T5929 17261-17269 NN denotes addition
T5930 17270-17272 TO denotes to
T5931 17273-17279 CD denotes TLR3/4
T5932 17280-17288 NNS denotes agonists
T5933 17288-17289 , denotes ,
T5934 17290-17293 DT denotes the
T5935 17294-17298 CD denotes TLR2
T5936 17299-17305 NN denotes ligand
T5937 17306-17313 NNS denotes Pam3CYS
T5938 17314-17317 VBD denotes was
T5939 17318-17322 VBN denotes used
T5940 17323-17328 IN denotes since
T5941 17329-17331 PRP denotes it
T5942 17332-17335 VBZ denotes has
T5943 17336-17340 VBN denotes been
T5944 17341-17346 VBN denotes shown
T5945 17347-17349 TO denotes to
T5946 17350-17356 VB denotes induce
T5947 17357-17361 DT denotes both
T5948 17362-17365 CD denotes Th1
T5949 17366-17369 CC denotes and
T5950 17370-17373 CD denotes Th2
T5951 17374-17383 NNS denotes responses
T5952 17384-17385 NNP denotes [
T5953 17385-17387 CD denotes 28
T5954 17387-17388 NNP denotes ]
T5955 17389-17390 NNP denotes [
T5956 17390-17392 CD denotes 30
T5957 17392-17393 NNP denotes ]
T5958 17393-17394 . denotes .
T5959 17395-17410 JJ denotes Pam3CYS-matured
T5960 17411-17413 NNP denotes WT
T5961 17414-17418 NNP denotes BMDC
T5962 17419-17426 VBD denotes induced
T5963 17427-17433 JJ denotes robust
T5964 17434-17438 JJ denotes IFNγ
T5965 17439-17449 NN denotes production
T5966 17450-17452 IN denotes at
T5967 17453-17454 DT denotes a
T5968 17455-17468 RB denotes significantly
T5969 17469-17475 JJR denotes higher
T5970 17476-17481 NN denotes level
T5971 17482-17486 IN denotes than
T5972 17487-17491 NN denotes cpdm
T5973 17492-17496 NNP denotes BMDC
T5974 17497-17498 -LRB- denotes (
T5975 17498-17502 NNP denotes Fig.
T5976 17503-17505 NN denotes 7A
T5977 17505-17506 -RRB- denotes )
T5978 17506-17507 . denotes .
T5979 17508-17511 DT denotes The
T5980 17512-17519 JJ denotes reduced
T5981 17520-17523 JJ denotes Th1
T5982 17524-17539 NN denotes differentiation
T5983 17540-17549 VBG denotes following
T5984 17550-17557 NNP denotes Pam3CYS
T5985 17558-17569 NN denotes stimulation
T5986 17570-17572 VBZ denotes is
T5987 17573-17583 JJ denotes consistent
T5988 17584-17588 IN denotes with
T5989 17589-17592 DT denotes the
T5990 17593-17599 JJ denotes recent
T5991 17600-17606 NN denotes report
T5992 17607-17609 IN denotes of
T5993 17610-17619 VBN denotes decreased
T5994 17620-17624 NN denotes IL12
T5995 17625-17635 NN denotes production
T5996 17636-17638 IN denotes in
T5997 17639-17643 NN denotes cpdm
T5998 17644-17655 NNS denotes macrophages
T5999 17656-17665 VBG denotes following
T6000 17666-17670 CD denotes TLR2
T6001 17671-17682 NN denotes stimulation
T6002 17683-17684 NNP denotes [
T6003 17684-17686 CD denotes 31
T6004 17686-17687 NNP denotes ]
T6005 17687-17688 . denotes .
T6006 17689-17691 IN denotes In
T6007 17692-17700 NN denotes contrast
T6008 17700-17701 , denotes ,
T6009 17702-17706 JJR denotes more
T6010 17707-17719 JJ denotes Th2-specific
T6011 17720-17723 NN denotes IL4
T6012 17724-17732 NN denotes cytokine
T6013 17733-17736 VBD denotes was
T6014 17737-17745 VBN denotes produced
T6015 17746-17748 IN denotes in
T6016 17749-17752 DT denotes the
T6017 17753-17757 NN denotes cpdm
T6018 17758-17762 NNP denotes BMDC
T6019 17763-17774 NNS denotes co-cultures
T6020 17775-17779 IN denotes than
T6021 17780-17783 DT denotes the
T6022 17784-17786 NNP denotes WT
T6023 17787-17794 NN denotes control
T6024 17795-17796 -LRB- denotes (
T6025 17796-17800 NNP denotes Fig.
T6026 17801-17803 NN denotes 7B
T6027 17803-17804 -RRB- denotes )
T6028 17804-17805 , denotes ,
T6029 17806-17816 VBG denotes suggesting
T6030 17817-17827 JJ denotes Th2-skewed
T6031 17828-17842 NN denotes immunogenicity
T6032 17843-17845 IN denotes of
T6033 17846-17850 NN denotes cpdm
T6034 17851-17855 NNP denotes BMDC
T6035 17855-17856 . denotes .
T6036 17857-17860 DT denotes The
T6037 17861-17871 NN denotes production
T6038 17872-17874 IN denotes of
T6039 17875-17888 JJ denotes Th17-specific
T6040 17889-17897 NN denotes cytokine
T6041 17898-17903 NNP denotes IL17A
T6042 17904-17913 VBG denotes following
T6043 17914-17917 NNP denotes LPS
T6044 17918-17929 NN denotes stimulation
T6045 17930-17932 IN denotes of
T6046 17933-17942 JJ denotes dendritic
T6047 17943-17948 NNS denotes cells
T6048 17949-17952 VBD denotes was
T6049 17953-17960 JJ denotes similar
T6050 17961-17968 IN denotes between
T6051 17969-17979 VBN denotes stimulated
T6052 17980-17982 NNP denotes WT
T6053 17983-17986 CC denotes and
T6054 17987-17991 VB denotes cpdm
T6055 17992-17996 NNP denotes BMDC
T6056 17997-18007 NNS denotes cocultures
T6057 18008-18009 -LRB- denotes (
T6058 18009-18013 NNS denotes data
T6059 18014-18017 RB denotes not
T6060 18018-18023 VBN denotes shown
T6061 18023-18024 -RRB- denotes )
T6062 18024-18025 . denotes .
T6063 18026-18033 IN denotes Despite
T6064 18034-18037 DT denotes the
T6065 18038-18046 JJ denotes distinct
T6066 18047-18050 SYM denotes Th1
T6067 18050-18051 : denotes -
T6068 18052-18055 CC denotes and
T6069 18056-18071 JJ denotes Th2-stimulating
T6070 18072-18081 NNS denotes abilities
T6071 18082-18084 IN denotes of
T6072 18085-18087 NNP denotes WT
T6073 18088-18091 CC denotes and
T6074 18092-18096 VB denotes cpdm
T6075 18097-18101 NNP denotes BMDC
T6076 18101-18102 , denotes ,
T6077 18103-18107 PRP denotes they
T6078 18108-18112 VBD denotes were
T6079 18113-18120 RB denotes equally
T6080 18121-18130 JJ denotes effective
T6081 18131-18133 IN denotes in
T6082 18134-18137 JJ denotes IL2
T6083 18138-18148 NN denotes production
T6084 18149-18153 IN denotes from
T6085 18154-18160 NNP denotes BMDC-T
T6086 18161-18165 NN denotes cell
T6087 18166-18177 NNS denotes co-cultures
T6088 18178-18184 IN denotes except
T6089 18185-18188 IN denotes for
T6090 18189-18193 NN denotes poly
T6091 18194-18195 PRP denotes I
T6092 18195-18196 : denotes :
T6093 18196-18197 NNP denotes C
T6094 18198-18209 NN denotes stimulation
T6095 18210-18215 WRB denotes where
T6096 18216-18218 NNP denotes WT
T6097 18219-18223 NNP denotes BMDC
T6098 18224-18231 VBD denotes induced
T6099 18232-18236 RBR denotes more
T6100 18237-18240 JJ denotes IL2
T6101 18241-18245 IN denotes than
T6102 18246-18250 NN denotes cpdm
T6103 18251-18255 NNP denotes BMDC
T6104 18256-18257 -LRB- denotes (
T6105 18257-18261 NNP denotes Fig.
T6106 18262-18264 NNP denotes 7C
T6107 18264-18265 -RRB- denotes )
T6108 18265-18266 . denotes .
T6109 18267-18270 DT denotes The
T6110 18271-18281 JJ denotes Th2-biased
T6111 18282-18293 VBG denotes stimulating
T6112 18294-18304 NN denotes capability
T6113 18305-18307 IN denotes of
T6114 18308-18312 NN denotes cpdm
T6115 18313-18317 NNP denotes BMDC
T6116 18318-18322 WRB denotes when
T6117 18323-18334 JJ denotes co-cultured
T6118 18335-18339 IN denotes with
T6119 18340-18350 JJ denotes allogeneic
T6120 18351-18356 NN denotes naïve
T6121 18357-18360 NNP denotes CD4
T6122 18360-18361 NNP denotes +
T6123 18362-18363 NNP denotes T
T6124 18364-18369 NNS denotes cells
T6125 18370-18372 VBZ denotes is
T6126 18373-18383 JJ denotes consistent
T6127 18384-18388 IN denotes with
T6128 18389-18392 DT denotes the
T6129 18393-18405 JJ denotes Th2-dominant
T6130 18406-18414 NN denotes cytokine
T6131 18415-18424 NN denotes phenotype
T6132 18425-18433 VBD denotes observed
T6133 18434-18436 IN denotes in
T6134 18437-18441 NN denotes cpdm
T6135 18442-18446 NNS denotes mice
T6136 18446-18447 . denotes .
T6805 19355-19358 DT denotes The
T6806 19359-19366 JJ denotes present
T6807 19367-19373 NN denotes report
T6808 19374-19380 VBD denotes showed
T6809 19381-19385 IN denotes that
T6810 19386-19390 JJ denotes null
T6811 19391-19400 NNS denotes mutations
T6812 19401-19403 IN denotes of
T6813 19404-19407 DT denotes the
T6814 19408-19413 NN denotes mouse
T6815 19414-19421 NNP denotes Sharpin
T6816 19422-19426 NN denotes gene
T6817 19427-19430 VBD denotes did
T6818 19431-19434 RB denotes not
T6819 19435-19441 VB denotes affect
T6820 19442-19445 DT denotes the
T6821 19446-19458 JJ denotes steady-state
T6822 19459-19471 NN denotes distribution
T6823 19472-19474 IN denotes of
T6824 19475-19482 JJ denotes splenic
T6825 19483-19485 NNP denotes DC
T6826 19486-19493 NNS denotes subsets
T6827 19494-19497 CC denotes nor
T6828 19498-19501 DT denotes the
T6829 19502-19513 NN denotes development
T6830 19514-19517 CC denotes and
T6831 19518-19527 NN denotes phenotype
T6832 19528-19530 IN denotes of
T6833 19531-19535 NNP denotes BMDC
T6834 19535-19536 . denotes .
T6835 19537-19544 RB denotes However
T6836 19544-19545 , denotes ,
T6837 19546-19550 NN denotes loss
T6838 19551-19553 IN denotes of
T6839 19554-19561 NNP denotes SHARPIN
T6840 19562-19575 RB denotes significantly
T6841 19576-19586 VBD denotes diminished
T6842 19587-19590 DT denotes the
T6843 19591-19599 NN denotes capacity
T6844 19600-19602 IN denotes of
T6845 19603-19607 NNP denotes BMDC
T6846 19608-19610 TO denotes to
T6847 19611-19618 VB denotes secrete
T6848 19619-19631 JJ denotes inflammatory
T6849 19632-19641 NNS denotes cytokines
T6850 19642-19645 CC denotes and
T6851 19646-19652 JJ denotes nitric
T6852 19653-19658 NN denotes oxide
T6853 19658-19659 . denotes .
T6854 19660-19663 DT denotes The
T6855 19664-19674 JJ denotes attenuated
T6856 19675-19683 NN denotes cytokine
T6857 19684-19694 NN denotes production
T6858 19695-19698 VBD denotes was
T6859 19699-19702 RB denotes not
T6860 19703-19706 JJ denotes due
T6861 19707-19709 TO denotes to
T6862 19710-19713 DT denotes the
T6863 19714-19722 NN denotes presence
T6864 19723-19725 IN denotes of
T6865 19726-19743 JJ denotes anti-inflammatory
T6866 19744-19754 NNS denotes inhibitors
T6867 19754-19755 , denotes ,
T6868 19756-19759 CC denotes and
T6869 19760-19763 MD denotes can
T6870 19764-19766 VB denotes be
T6871 19767-19774 RB denotes largely
T6872 19775-19784 VBN denotes explained
T6873 19785-19787 IN denotes by
T6874 19788-19797 JJ denotes selective
T6875 19798-19810 NN denotes inactivation
T6876 19811-19813 IN denotes of
T6877 19814-19819 JJ denotes NF-κB
T6878 19820-19829 VBG denotes signaling
T6879 19829-19830 . denotes .
T6880 19831-19841 VBN denotes Stimulated
T6881 19842-19846 NN denotes cpdm
T6882 19847-19851 NNP denotes BMDC
T6883 19852-19861 VBD denotes exhibited
T6884 19862-19872 JJ denotes Th2-biased
T6885 19873-19874 NNP denotes T
T6886 19875-19890 JJ denotes cell-polarizing
T6887 19891-19903 NNS denotes capabilities
T6888 19903-19904 , denotes ,
T6889 19905-19915 JJ denotes consistent
T6890 19916-19920 IN denotes with
T6891 19921-19924 DT denotes the
T6892 19925-19928 NNP denotes Th2
T6893 19929-19946 JJ denotes cytokine-dominant
T6894 19947-19956 NN denotes phenotype
T6895 19957-19959 IN denotes in
T6896 19960-19964 NN denotes cpdm
T6897 19965-19969 NNS denotes mice
T6898 19969-19970 . denotes .
T6899 19971-19979 RB denotes Together
T6900 19979-19980 , denotes ,
T6901 19981-19986 DT denotes these
T6902 19987-19994 NNS denotes results
T6903 19995-20003 VBP denotes indicate
T6904 20004-20006 DT denotes an
T6905 20007-20020 JJ denotes indispensible
T6906 20021-20025 NN denotes role
T6907 20026-20028 IN denotes of
T6908 20029-20036 NNP denotes SHARPIN
T6909 20037-20039 IN denotes in
T6910 20040-20050 VBG denotes regulating
T6911 20051-20053 NNP denotes DC
T6912 20054-20067 JJ denotes immunological
T6913 20068-20077 NNS denotes functions
T6914 20077-20078 , denotes ,
T6915 20079-20089 NN denotes disruption
T6916 20090-20092 IN denotes of
T6917 20093-20098 WDT denotes which
T6918 20099-20102 MD denotes may
T6919 20103-20113 VB denotes contribute
T6920 20114-20116 TO denotes to
T6921 20117-20120 DT denotes the
T6922 20121-20132 NN denotes development
T6923 20133-20135 IN denotes of
T6924 20136-20142 JJ denotes immune
T6925 20143-20151 NNS denotes diseases
T6926 20151-20152 . denotes .
T6927 20153-20158 IN denotes Since
T6928 20159-20161 NNP denotes WT
T6929 20162-20165 CC denotes and
T6930 20166-20170 NN denotes cpdm
T6931 20171-20175 NNS denotes mice
T6932 20176-20179 VBP denotes are
T6933 20180-20184 DT denotes both
T6934 20185-20193 JJ denotes specific
T6935 20194-20207 NN denotes pathogen-free
T6936 20207-20208 , denotes ,
T6937 20209-20212 DT denotes the
T6938 20213-20219 NN denotes nature
T6939 20220-20222 IN denotes of
T6940 20223-20226 DT denotes the
T6941 20227-20234 NN denotes trigger
T6942 20235-20237 IN denotes of
T6943 20238-20241 DT denotes the
T6944 20242-20248 JJ denotes severe
T6945 20249-20261 NN denotes inflammation
T6946 20262-20264 IN denotes in
T6947 20265-20269 NN denotes cpdm
T6948 20270-20274 NNS denotes mice
T6949 20275-20277 VBZ denotes is
T6950 20278-20281 RB denotes not
T6951 20282-20289 JJ denotes obvious
T6952 20289-20290 . denotes .
T6953 20291-20294 CD denotes One
T6954 20295-20299 JJ denotes such
T6955 20300-20310 VBG denotes initiating
T6956 20311-20317 NN denotes factor
T6957 20318-20323 MD denotes could
T6958 20324-20326 VB denotes be
T6959 20327-20337 JJ denotes endogenous
T6960 20338-20347 JJ denotes apoptotic
T6961 20348-20354 CC denotes and/or
T6962 20355-20363 JJ denotes necrotic
T6963 20364-20369 NNS denotes cells
T6964 20370-20374 WDT denotes that
T6965 20375-20378 MD denotes can
T6966 20379-20386 VB denotes release
T6967 20387-20404 JJ denotes danger-associated
T6968 20405-20414 JJ denotes molecular
T6969 20415-20423 NNS denotes patterns
T6970 20424-20425 -LRB- denotes (
T6971 20425-20430 NNS denotes DAMPs
T6972 20430-20431 -RRB- denotes )
T6973 20432-20434 TO denotes to
T6974 20435-20441 VB denotes launch
T6975 20442-20445 CC denotes and
T6976 20446-20453 VB denotes amplify
T6977 20454-20456 DT denotes an
T6978 20457-20469 JJ denotes inflammatory
T6979 20470-20478 NN denotes response
T6980 20479-20480 NNP denotes [
T6981 20480-20482 CD denotes 32
T6982 20482-20483 NNP denotes ]
T6983 20483-20484 . denotes .
T6984 20485-20489 JJ denotes Such
T6985 20491-20498 JJ denotes sterile
T6986 20500-20512 NN denotes inflammation
T6987 20513-20518 MD denotes could
T6988 20519-20521 VB denotes be
T6989 20522-20531 VBN denotes initiated
T6990 20532-20535 CC denotes and
T6991 20536-20540 VB denotes take
T6992 20541-20546 NN denotes place
T6993 20547-20549 IN denotes in
T6994 20550-20553 DT denotes all
T6995 20554-20560 NNS denotes organs
T6996 20561-20564 CC denotes and
T6997 20565-20572 NNS denotes tissues
T6998 20573-20581 VBN denotes affected
T6999 20582-20584 IN denotes in
T7000 20585-20589 NN denotes cpdm
T7001 20590-20594 NNS denotes mice
T7002 20594-20595 , denotes ,
T7003 20596-20600 RB denotes thus
T7004 20601-20608 VBG denotes causing
T7005 20609-20620 JJ denotes multi-organ
T7006 20621-20633 JJ denotes inflammatory
T7007 20634-20643 NNS denotes disorders
T7008 20643-20644 . denotes .
T7009 20645-20649 DT denotes This
T7010 20650-20660 NN denotes hypothesis
T7011 20661-20663 VBZ denotes is
T7012 20664-20673 VBN denotes supported
T7013 20674-20676 IN denotes by
T7014 20677-20684 JJ denotes several
T7015 20685-20691 JJ denotes recent
T7016 20692-20699 NNS denotes studies
T7017 20699-20700 . denotes .
T7018 20701-20712 NNS denotes Fibroblasts
T7019 20713-20715 IN denotes of
T7020 20716-20720 NN denotes cpdm
T7021 20721-20725 NNS denotes mice
T7022 20726-20729 VBP denotes are
T7023 20730-20736 RB denotes highly
T7024 20737-20746 JJ denotes sensitive
T7025 20747-20749 TO denotes to
T7026 20750-20762 JJ denotes TNFα-induced
T7027 20763-20767 NN denotes cell
T7028 20768-20773 NN denotes death
T7029 20774-20777 CC denotes and
T7030 20778-20781 DT denotes the
T7031 20782-20786 NN denotes cpdm
T7032 20787-20796 NN denotes phenotype
T7033 20797-20800 MD denotes can
T7034 20801-20803 VB denotes be
T7035 20804-20813 RB denotes partially
T7036 20814-20821 VBN denotes rescued
T7037 20822-20824 IN denotes by
T7038 20825-20833 NN denotes deletion
T7039 20834-20836 IN denotes of
T7040 20837-20840 NNP denotes TNF
T7041 20841-20842 NNP denotes [
T7042 20842-20843 CD denotes 7
T7043 20843-20844 NNP denotes ]
T7044 20844-20845 , denotes ,
T7045 20846-20847 NNP denotes [
T7046 20847-20848 CD denotes 8
T7047 20848-20849 NNP denotes ]
T7048 20849-20850 , denotes ,
T7049 20851-20861 VBG denotes suggesting
T7050 20862-20866 IN denotes that
T7051 20867-20877 NN denotes deficiency
T7052 20878-20880 IN denotes of
T7053 20881-20888 NNP denotes SHARPIN
T7054 20889-20900 VBZ denotes compromises
T7055 20901-20904 DT denotes the
T7056 20905-20919 JJ denotes anti-apoptotic
T7057 20920-20930 NNS denotes mechanisms
T7058 20931-20933 IN denotes in
T7059 20934-20938 NN denotes cpdm
T7060 20939-20943 NNS denotes mice
T7061 20944-20953 VBG denotes resulting
T7062 20954-20956 IN denotes in
T7063 20957-20961 NN denotes cell
T7064 20962-20975 JJ denotes death-induced
T7065 20976-20988 JJ denotes inflammatory
T7066 20989-20996 NN denotes disease
T7067 20996-20997 . denotes .
T7068 20998-21007 NNP denotes Apoptosis
T7069 21008-21010 IN denotes of
T7070 21011-21024 NNS denotes keratinocytes
T7071 21025-21027 VBZ denotes is
T7072 21028-21029 DT denotes a
T7073 21030-21039 JJ denotes prominent
T7074 21040-21047 NN denotes feature
T7075 21048-21050 IN denotes of
T7076 21051-21054 DT denotes the
T7077 21055-21059 NN denotes skin
T7078 21060-21067 NNS denotes lesions
T7079 21068-21070 IN denotes in
T7080 21071-21075 NN denotes cpdm
T7081 21076-21080 NNS denotes mice
T7082 21081-21082 NNP denotes [
T7083 21082-21084 CD denotes 33
T7084 21084-21085 NNP denotes ]
T7085 21086-21089 CC denotes and
T7086 21090-21094 DT denotes this
T7087 21095-21097 VBZ denotes is
T7088 21098-21106 VBN denotes mediated
T7089 21107-21109 IN denotes by
T7090 21110-21127 JJ denotes caspase-dependent
T7091 21128-21141 JJ denotes mitochondrial
T7092 21142-21150 NNS denotes pathways
T7093 21151-21152 NNP denotes [
T7094 21152-21154 CD denotes 34
T7095 21154-21155 NNP denotes ]
T7096 21155-21156 . denotes .
T7097 21157-21162 DT denotes These
T7098 21163-21170 JJ denotes induced
T7099 21171-21177 CC denotes and/or
T7100 21178-21187 JJ denotes intrinsic
T7101 21188-21197 JJ denotes apoptotic
T7102 21198-21203 NNS denotes cells
T7103 21204-21207 MD denotes can
T7104 21208-21215 VB denotes release
T7105 21216-21223 JJ denotes various
T7106 21224-21229 NNS denotes types
T7107 21230-21232 IN denotes of
T7108 21233-21238 NNS denotes DAMPs
T7109 21239-21243 WDT denotes that
T7110 21244-21249 VBP denotes exert
T7111 21250-21255 PRP$ denotes their
T7112 21256-21272 JJ denotes pro-inflammatory
T7113 21273-21283 NNS denotes properties
T7114 21284-21286 IN denotes by
T7115 21287-21297 VBG denotes activating
T7116 21298-21300 NNP denotes DC
T7117 21301-21308 IN denotes through
T7118 21309-21316 NN denotes pattern
T7119 21317-21328 NN denotes recognition
T7120 21329-21338 NNS denotes receptors
T7121 21339-21340 NNP denotes [
T7122 21340-21342 CD denotes 32
T7123 21342-21343 NNP denotes ]
T7124 21343-21344 , denotes ,
T7125 21345-21346 NNP denotes [
T7126 21346-21348 CD denotes 35
T7127 21348-21349 NNP denotes ]
T7128 21349-21350 , denotes ,
T7129 21351-21355 JJ denotes such
T7130 21356-21358 IN denotes as
T7131 21359-21364 CD denotes HMGB1
T7132 21365-21375 VBN denotes recognized
T7133 21376-21378 IN denotes by
T7134 21379-21385 CD denotes TLR2/4
T7135 21386-21387 NNP denotes [
T7136 21387-21389 CD denotes 36
T7137 21389-21390 NNP denotes ]
T7138 21390-21391 , denotes ,
T7139 21392-21393 NNP denotes [
T7140 21393-21395 CD denotes 37
T7141 21395-21396 NNP denotes ]
T7142 21396-21397 . denotes .
T7143 21398-21408 JJ denotes Consistent
T7144 21409-21413 IN denotes with
T7145 21414-21417 DT denotes the
T7146 21418-21426 VBN denotes impaired
T7147 21427-21430 NNP denotes Th1
T7148 21431-21437 JJ denotes immune
T7149 21438-21446 NN denotes response
T7150 21447-21449 IN denotes in
T7151 21450-21454 NN denotes cpdm
T7152 21455-21459 NNS denotes mice
T7153 21460-21461 VBP denotes [
T7154 21461-21462 CD denotes 5
T7155 21462-21463 NNP denotes ]
T7156 21463-21464 , denotes ,
T7157 21465-21475 VBD denotes stimulated
T7158 21476-21480 NNP denotes BMDC
T7159 21481-21487 RB denotes weakly
T7160 21488-21497 VBD denotes polarized
T7161 21498-21501 CD denotes Th1
T7162 21502-21517 NN denotes differentiation
T7163 21517-21518 , denotes ,
T7164 21519-21522 CC denotes but
T7165 21523-21531 RB denotes strongly
T7166 21532-21541 VBD denotes supported
T7167 21542-21545 DT denotes the
T7168 21546-21557 NN denotes development
T7169 21558-21560 IN denotes of
T7170 21561-21564 JJ denotes Th2
T7171 21565-21573 NN denotes effector
T7172 21574-21579 NNS denotes cells
T7173 21579-21580 . denotes .
T7174 21581-21589 VBN denotes Combined
T7175 21590-21594 IN denotes with
T7176 21595-21598 DT denotes the
T7177 21599-21607 JJ denotes dramatic
T7178 21608-21614 NN denotes effect
T7179 21615-21617 IN denotes of
T7180 21618-21622 CD denotes IL12
T7181 21623-21632 NN denotes treatment
T7182 21633-21635 IN denotes on
T7183 21636-21639 DT denotes the
T7184 21640-21649 NN denotes phenotype
T7185 21650-21652 IN denotes of
T7186 21653-21658 DT denotes these
T7187 21659-21663 NNS denotes mice
T7188 21664-21665 VBP denotes [
T7189 21665-21666 CD denotes 5
T7190 21666-21667 NNP denotes ]
T7191 21667-21668 , denotes ,
T7192 21669-21673 DT denotes this
T7193 21674-21682 VBZ denotes suggests
T7194 21683-21687 IN denotes that
T7195 21688-21691 DT denotes the
T7196 21692-21702 JJ denotes Th2-biased
T7197 21703-21711 JJ denotes systemic
T7198 21712-21724 NN denotes inflammation
T7199 21725-21727 IN denotes in
T7200 21728-21732 NN denotes cpdm
T7201 21733-21737 NNS denotes mice
T7202 21738-21740 VBZ denotes is
T7203 21741-21747 VBN denotes caused
T7204 21748-21750 IN denotes by
T7205 21751-21758 JJ denotes reduced
T7206 21759-21766 JJ denotes IL12P70
T7207 21767-21777 NN denotes production
T7208 21778-21782 IN denotes from
T7209 21783-21785 NNP denotes DC
T7210 21785-21786 . denotes .
T7211 21787-21790 DT denotes The
T7212 21791-21801 NN denotes importance
T7213 21802-21804 IN denotes of
T7214 21805-21812 NNP denotes IL12P70
T7215 21813-21815 IN denotes in
T7216 21816-21826 VBG denotes regulating
T7217 21827-21830 NNP denotes Th1
T7218 21831-21834 CC denotes and
T7219 21835-21838 NNP denotes Th2
T7220 21839-21845 JJ denotes immune
T7221 21846-21855 NNS denotes responses
T7222 21856-21858 IN denotes in
T7223 21859-21863 NNS denotes mice
T7224 21864-21867 VBD denotes was
T7225 21868-21875 RB denotes clearly
T7226 21876-21888 VBN denotes demonstrated
T7227 21889-21891 IN denotes in
T7228 21892-21899 NNP denotes IL12P35
T7229 21899-21900 : denotes -
T7230 21901-21904 CC denotes and
T7231 21905-21922 JJ denotes IL12P40-deficient
T7232 21923-21927 NNS denotes mice
T7233 21928-21929 NNP denotes [
T7234 21929-21931 CD denotes 38
T7235 21931-21932 NNP denotes ]
T7236 21932-21933 , denotes ,
T7237 21934-21935 NNP denotes [
T7238 21935-21937 CD denotes 39
T7239 21937-21938 NNP denotes ]
T7240 21939-21942 CC denotes and
T7241 21943-21950 IN denotes through
T7242 21951-21959 JJ denotes clinical
T7243 21960-21967 NNS denotes studies
T7244 21968-21970 IN denotes in
T7245 21971-21976 JJ denotes human
T7246 21977-21985 NNS denotes patients
T7247 21986-21987 NNP denotes [
T7248 21987-21989 CD denotes 40
T7249 21989-21990 NNP denotes ]
T7250 21991-21992 NNP denotes [
T7251 21992-21994 CD denotes 42
T7252 21994-21995 NNP denotes ]
T7253 21995-21996 . denotes .
T7254 21997-22002 NN denotes NF-κB
T7255 22002-22003 , denotes ,
T7256 22004-22012 CD denotes TBK/IRF3
T7257 22012-22013 , denotes ,
T7258 22014-22017 CC denotes and
T7259 22018-22022 NNP denotes MAPK
T7260 22023-22032 VBG denotes signaling
T7261 22033-22036 VBP denotes are
T7262 22037-22046 JJ denotes important
T7263 22047-22055 NNS denotes pathways
T7264 22056-22065 VBN denotes activated
T7265 22066-22068 IN denotes by
T7266 22069-22072 NNP denotes LPS
T7267 22073-22075 CC denotes or
T7268 22076-22080 RB denotes poly
T7269 22081-22082 PRP denotes I
T7270 22082-22083 : denotes :
T7271 22083-22084 NNP denotes C
T7272 22085-22088 CC denotes and
T7273 22089-22099 NN denotes disruption
T7274 22100-22102 IN denotes of
T7275 22103-22109 DT denotes either
T7276 22110-22117 NN denotes pathway
T7277 22118-22121 MD denotes may
T7278 22122-22126 VB denotes lead
T7279 22127-22129 TO denotes to
T7280 22130-22139 VBN denotes decreased
T7281 22140-22148 NN denotes cytokine
T7282 22149-22159 NN denotes expression
T7283 22159-22160 . denotes .
T7284 22161-22166 JJ denotes NF-κB
T7285 22167-22177 NN denotes activation
T7286 22178-22181 VBD denotes was
T7287 22182-22193 RB denotes selectively
T7288 22194-22203 VBN denotes inhibited
T7289 22204-22206 IN denotes in
T7290 22207-22210 NNP denotes LPS
T7291 22211-22214 CC denotes and
T7292 22215-22219 RB denotes poly
T7293 22220-22221 PRP denotes I
T7294 22221-22222 : denotes :
T7295 22222-22223 NNP denotes C
T7296 22224-22234 VBD denotes stimulated
T7297 22235-22239 NN denotes cpdm
T7298 22240-22244 NNP denotes BMDC
T7299 22245-22253 VBN denotes compared
T7300 22254-22256 TO denotes to
T7301 22257-22261 JJ denotes wild
T7302 22262-22266 NN denotes type
T7303 22266-22267 , denotes ,
T7304 22268-22273 IN denotes while
T7305 22274-22284 NN denotes activation
T7306 22285-22287 IN denotes of
T7307 22288-22297 CD denotes TBK1/IRF3
T7308 22297-22298 , denotes ,
T7309 22299-22305 NNP denotes ERK1/2
T7310 22305-22306 , denotes ,
T7311 22307-22309 CC denotes or
T7312 22310-22313 CD denotes p38
T7313 22314-22317 VBD denotes was
T7314 22318-22321 RB denotes not
T7315 22321-22322 , denotes ,
T7316 22323-22333 VBG denotes indicating
T7317 22334-22338 IN denotes that
T7318 22339-22348 VBN denotes disrupted
T7319 22349-22354 NNP denotes NF-κB
T7320 22355-22364 VBG denotes signaling
T7321 22365-22367 IN denotes in
T7322 22368-22372 NN denotes cpdm
T7323 22373-22375 NNP denotes DC
T7324 22376-22378 VBZ denotes is
T7325 22379-22390 JJ denotes responsible
T7326 22391-22394 IN denotes for
T7327 22395-22398 DT denotes the
T7328 22399-22408 JJ denotes defective
T7329 22409-22417 NN denotes cytokine
T7330 22418-22428 NN denotes expression
T7331 22428-22429 . denotes .
T7332 22430-22435 DT denotes These
T7333 22436-22443 NNS denotes results
T7334 22444-22448 VBP denotes seem
T7335 22449-22451 TO denotes to
T7336 22452-22462 VB denotes contradict
T7337 22463-22464 DT denotes a
T7338 22465-22471 JJ denotes recent
T7339 22472-22477 NN denotes study
T7340 22478-22482 WDT denotes that
T7341 22483-22488 VBD denotes found
T7342 22489-22498 VBN denotes increased
T7343 22499-22505 NNS denotes levels
T7344 22506-22508 IN denotes of
T7345 22509-22514 JJ denotes NF-κB
T7346 22515-22525 NN denotes activation
T7347 22526-22529 CC denotes and
T7348 22530-22533 CD denotes IL1
T7349 22534-22547 NN denotes transcription
T7350 22548-22550 IN denotes in
T7351 22551-22555 NN denotes cpdm
T7352 22556-22560 NNS denotes mice
T7353 22561-22562 NNP denotes [
T7354 22562-22564 CD denotes 43
T7355 22564-22565 NNP denotes ]
T7356 22565-22566 . denotes .
T7357 22567-22571 DT denotes This
T7358 22572-22582 NN denotes difference
T7359 22583-22586 MD denotes can
T7360 22587-22595 RB denotes probably
T7361 22596-22598 VB denotes be
T7362 22599-22609 VBN denotes attributed
T7363 22610-22612 TO denotes to
T7364 22613-22616 DT denotes the
T7365 22617-22626 JJ denotes different
T7366 22627-22632 NNS denotes cells
T7367 22633-22636 CC denotes and
T7368 22637-22644 NNS denotes tissues
T7369 22645-22649 VBN denotes used
T7370 22650-22652 IN denotes in
T7371 22653-22658 DT denotes these
T7372 22659-22666 NNS denotes studies
T7373 22666-22667 , denotes ,
T7374 22668-22671 CC denotes and
T7375 22672-22675 MD denotes may
T7376 22676-22681 VB denotes point
T7377 22682-22684 TO denotes to
T7378 22685-22689 NN denotes cell
T7379 22689-22690 : denotes -
T7380 22691-22694 CC denotes and
T7381 22695-22701 NN denotes tissue
T7382 22701-22702 : denotes -
T7383 22703-22707 NN denotes type
T7384 22708-22716 JJ denotes specific
T7385 22717-22726 NNS denotes functions
T7386 22727-22729 IN denotes of
T7387 22730-22737 NNP denotes SHARPIN
T7388 22737-22738 . denotes .
T7389 22739-22743 DT denotes This
T7390 22744-22746 VBZ denotes is
T7391 22747-22757 JJ denotes consistent
T7392 22758-22762 IN denotes with
T7393 22763-22769 JJ denotes recent
T7394 22770-22777 NNS denotes reports
T7395 22778-22780 IN denotes of
T7396 22781-22796 JJ denotes tissue-specific
T7397 22797-22804 NNS denotes effects
T7398 22805-22807 IN denotes of
T7399 22808-22813 JJ denotes NF-κB
T7400 22814-22823 VBG denotes signaling
T7401 22824-22825 NNP denotes [
T7402 22825-22827 CD denotes 44
T7403 22827-22828 NNP denotes ]
T7404 22828-22829 . denotes .
T7405 22830-22840 JJ denotes Ubiquitous
T7406 22841-22851 NN denotes activation
T7407 22852-22854 IN denotes of
T7408 22855-22860 NN denotes NF-κB
T7409 22861-22863 IN denotes by
T7410 22864-22872 VBG denotes removing
T7411 22873-22883 NNS denotes inhibitors
T7412 22884-22888 JJ denotes such
T7413 22889-22891 IN denotes as
T7414 22892-22895 NNP denotes A20
T7415 22896-22899 CC denotes and
T7416 22900-22904 NNP denotes ITCH
T7417 22905-22912 IN denotes through
T7418 22913-22920 JJ denotes genetic
T7419 22921-22933 NN denotes manipulation
T7420 22934-22941 NNS denotes results
T7421 22942-22944 IN denotes in
T7422 22945-22955 JJ denotes widespread
T7423 22956-22968 NN denotes inflammation
T7424 22968-22969 , denotes ,
T7425 22970-22980 JJ denotes consistent
T7426 22981-22985 IN denotes with
T7427 22986-22989 DT denotes the
T7428 22990-22994 NN denotes role
T7429 22995-22997 IN denotes of
T7430 22998-23003 NN denotes NF-κB
T7431 23004-23006 IN denotes in
T7432 23007-23010 DT denotes the
T7433 23011-23021 NN denotes production
T7434 23022-23024 IN denotes of
T7435 23025-23041 JJ denotes pro-inflammatory
T7436 23042-23051 NNS denotes mediators
T7437 23052-23053 NNP denotes [
T7438 23053-23055 CD denotes 45
T7439 23055-23056 NNP denotes ]
T7440 23056-23057 , denotes ,
T7441 23058-23059 NNP denotes [
T7442 23059-23061 CD denotes 46
T7443 23061-23062 NNP denotes ]
T7444 23062-23063 . denotes .
T7445 23064-23071 RB denotes However
T7446 23071-23072 , denotes ,
T7447 23073-23082 JJ denotes selective
T7448 23083-23093 NN denotes inhibition
T7449 23094-23096 IN denotes of
T7450 23097-23102 JJ denotes NF-κB
T7451 23103-23113 NN denotes activation
T7452 23114-23116 IN denotes in
T7453 23117-23128 JJ denotes parenchymal
T7454 23129-23134 NNS denotes cells
T7455 23135-23137 IN denotes of
T7456 23138-23141 DT denotes the
T7457 23142-23146 NN denotes skin
T7458 23146-23147 , denotes ,
T7459 23148-23153 NN denotes liver
T7460 23153-23154 , denotes ,
T7461 23155-23158 CC denotes and
T7462 23159-23168 JJ denotes intestine
T7463 23169-23176 NNS denotes results
T7464 23177-23179 IN denotes in
T7465 23180-23187 JJ denotes chronic
T7466 23188-23200 NN denotes inflammation
T7467 23201-23207 VBN denotes driven
T7468 23208-23210 IN denotes by
T7469 23211-23216 JJ denotes NF-κB
T7470 23217-23226 JJ denotes competent
T7471 23227-23237 NNS denotes leukocytes
T7472 23238-23239 NNP denotes [
T7473 23239-23241 CD denotes 47
T7474 23241-23242 NNP denotes ]
T7475 23243-23244 NNP denotes [
T7476 23244-23246 CD denotes 50
T7477 23246-23247 NNP denotes ]
T7478 23247-23248 . denotes .
T7479 23249-23253 DT denotes This
T7480 23254-23263 VBZ denotes indicates
T7481 23264-23268 IN denotes that
T7482 23269-23270 DT denotes a
T7483 23271-23278 NN denotes balance
T7484 23279-23286 IN denotes between
T7485 23287-23290 DT denotes the
T7486 23291-23307 JJ denotes pro-inflammatory
T7487 23308-23312 NN denotes role
T7488 23313-23315 IN denotes of
T7489 23316-23321 NN denotes NF-κB
T7490 23322-23324 IN denotes in
T7491 23325-23335 NNS denotes leukocytes
T7492 23336-23339 CC denotes and
T7493 23340-23343 DT denotes the
T7494 23344-23361 JJ denotes anti-inflammatory
T7495 23362-23366 NN denotes role
T7496 23367-23369 IN denotes in
T7497 23370-23381 JJ denotes parenchymal
T7498 23382-23387 NNS denotes cells
T7499 23388-23390 VBZ denotes is
T7500 23391-23399 JJ denotes critical
T7501 23400-23402 IN denotes in
T7502 23403-23406 DT denotes the
T7503 23407-23418 NN denotes maintenance
T7504 23419-23421 IN denotes of
T7505 23422-23428 NN denotes tissue
T7506 23429-23440 NN denotes homeostasis
T7507 23440-23441 . denotes .
T7508 23442-23453 NNS denotes Experiments
T7509 23454-23458 IN denotes with
T7510 23459-23463 NNS denotes mice
T7511 23464-23468 IN denotes with
T7512 23469-23473 NN denotes cell
T7513 23473-23474 : denotes -
T7514 23475-23478 CC denotes and
T7515 23479-23494 JJ denotes tissue-specific
T7516 23495-23503 NN denotes deletion
T7517 23504-23506 IN denotes of
T7518 23507-23514 NNP denotes Sharpin
T7519 23514-23515 , denotes ,
T7520 23516-23525 RB denotes currently
T7521 23526-23531 IN denotes under
T7522 23532-23535 NN denotes way
T7523 23535-23536 , denotes ,
T7524 23537-23541 MD denotes will
T7525 23542-23546 VB denotes help
T7526 23547-23549 TO denotes to
T7527 23550-23559 VB denotes elucidate
T7528 23560-23563 DT denotes the
T7529 23564-23568 NN denotes role
T7530 23569-23571 IN denotes of
T7531 23572-23579 NNP denotes SHARPIN
T7532 23580-23582 IN denotes in
T7533 23583-23595 NN denotes inflammation
T7534 23595-23596 . denotes .
T7535 23597-23604 IN denotes Despite
T7536 23605-23614 JJ denotes defective
T7537 23615-23620 NN denotes NF-κB
T7538 23621-23631 NN denotes activation
T7539 23632-23634 IN denotes in
T7540 23635-23638 DT denotes the
T7541 23639-23646 NN denotes absence
T7542 23647-23649 IN denotes of
T7543 23650-23657 NNP denotes Sharpin
T7544 23658-23668 NN denotes expression
T7545 23668-23669 , denotes ,
T7546 23670-23675 EX denotes there
T7547 23676-23679 VBD denotes was
T7548 23680-23682 DT denotes no
T7549 23683-23694 JJ denotes significant
T7550 23695-23701 NN denotes change
T7551 23702-23704 IN denotes in
T7552 23705-23712 JJ denotes splenic
T7553 23713-23715 NNP denotes DC
T7554 23716-23727 NNS denotes populations
T7555 23728-23730 CC denotes or
T7556 23731-23741 NN denotes expression
T7557 23742-23744 IN denotes of
T7558 23745-23759 JJ denotes co-stimulatory
T7559 23760-23769 NNS denotes molecules
T7560 23770-23772 IN denotes on
T7561 23773-23777 NNP denotes BMDC
T7562 23777-23778 . denotes .
T7563 23779-23788 JJ denotes Different
T7564 23789-23794 NN denotes NF-κB
T7565 23795-23803 NNS denotes subunits
T7566 23804-23812 VBN denotes involved
T7567 23813-23815 IN denotes in
T7568 23816-23819 DT denotes the
T7569 23820-23829 JJ denotes canonical
T7570 23830-23833 CC denotes and
T7571 23834-23847 JJ denotes non-canonical
T7572 23848-23856 NNS denotes branches
T7573 23857-23859 IN denotes of
T7574 23860-23863 DT denotes the
T7575 23864-23869 NNP denotes NF-κB
T7576 23870-23879 VBG denotes signaling
T7577 23880-23887 NN denotes pathway
T7578 23888-23892 VBP denotes have
T7579 23893-23901 JJ denotes distinct
T7580 23902-23911 NNS denotes functions
T7581 23912-23914 TO denotes to
T7582 23915-23922 VB denotes control
T7583 23923-23931 JJ denotes specific
T7584 23932-23939 NNS denotes aspects
T7585 23940-23942 IN denotes of
T7586 23943-23945 NNP denotes DC
T7587 23946-23957 NN denotes development
T7588 23958-23961 CC denotes and
T7589 23962-23970 NN denotes function
T7590 23971-23972 NNP denotes [
T7591 23972-23974 CD denotes 51
T7592 23974-23975 NNP denotes ]
T7593 23975-23976 , denotes ,
T7594 23977-23978 NNP denotes [
T7595 23978-23980 CD denotes 52
T7596 23980-23981 NNP denotes ]
T7597 23981-23982 . denotes .
T7598 23983-23986 DT denotes The
T7599 23987-24000 JJ denotes non-canonical
T7600 24001-24008 NN denotes pathway
T7601 24009-24010 -LRB- denotes (
T7602 24010-24014 JJ denotes p100
T7603 24015-24025 NN denotes processing
T7604 24026-24028 TO denotes to
T7605 24029-24036 VB denotes produce
T7606 24037-24040 CD denotes p52
T7607 24040-24041 -RRB- denotes )
T7608 24042-24049 VBZ denotes appears
T7609 24050-24052 TO denotes to
T7610 24053-24055 VB denotes be
T7611 24056-24062 JJ denotes intact
T7612 24063-24065 IN denotes in
T7613 24066-24083 JJ denotes SHARPIN-deficient
T7614 24084-24089 NNS denotes cells
T7615 24090-24091 VBP denotes [
T7616 24091-24092 CD denotes 7
T7617 24092-24093 NNP denotes ]
T7618 24093-24094 , denotes ,
T7619 24095-24096 NNP denotes [
T7620 24096-24097 CD denotes 9
T7621 24097-24098 NNP denotes ]
T7622 24099-24109 VBG denotes suggesting
T7623 24110-24114 IN denotes that
T7624 24115-24118 DT denotes the
T7625 24119-24124 JJ denotes NF-κB
T7626 24125-24136 NN denotes heterodimer
T7627 24137-24145 NN denotes p52/RELB
T7628 24146-24148 VBZ denotes is
T7629 24149-24159 JJ denotes sufficient
T7630 24160-24162 TO denotes to
T7631 24163-24171 VB denotes maintain
T7632 24172-24175 DT denotes the
T7633 24176-24182 JJ denotes normal
T7634 24183-24193 NN denotes regulation
T7635 24194-24196 IN denotes of
T7636 24197-24199 NNP denotes DC
T7637 24200-24211 NN denotes homeostasis
T7638 24212-24215 CC denotes and
T7639 24216-24226 NN denotes maturation
T7640 24226-24227 . denotes .
T7641 24228-24231 DT denotes The
T7642 24232-24241 JJ denotes molecular
T7643 24242-24247 NN denotes basis
T7644 24248-24250 IN denotes by
T7645 24251-24256 WDT denotes which
T7646 24257-24260 DT denotes the
T7647 24261-24268 NNP denotes Sharpin
T7648 24269-24277 NN denotes mutation
T7649 24278-24284 VBZ denotes causes
T7650 24285-24292 VBN denotes reduced
T7651 24293-24298 JJ denotes NF-κB
T7652 24299-24309 NN denotes activation
T7653 24310-24312 IN denotes in
T7654 24313-24317 NNP denotes BMDC
T7655 24318-24325 VBZ denotes remains
T7656 24326-24328 TO denotes to
T7657 24329-24331 VB denotes be
T7658 24332-24342 VBN denotes determined
T7659 24342-24343 . denotes .
T7660 24344-24347 NNP denotes LPS
T7661 24348-24351 CC denotes and
T7662 24352-24356 RB denotes poly
T7663 24357-24358 PRP denotes I
T7664 24358-24359 : denotes :
T7665 24359-24360 NNP denotes C
T7666 24361-24365 VBD denotes used
T7667 24366-24370 RB denotes here
T7668 24371-24374 VBP denotes are
T7669 24375-24387 JJ denotes well-defined
T7670 24388-24395 NNS denotes ligands
T7671 24396-24400 WDT denotes that
T7672 24401-24413 RB denotes specifically
T7673 24414-24420 VBP denotes engage
T7674 24421-24425 CD denotes TLR4
T7675 24426-24429 CC denotes and
T7676 24430-24434 CD denotes TLR3
T7677 24434-24435 , denotes ,
T7678 24436-24448 RB denotes respectively
T7679 24448-24449 . denotes .
T7680 24450-24453 DT denotes The
T7681 24454-24464 NN denotes expression
T7682 24465-24472 NN denotes profile
T7683 24473-24475 IN denotes of
T7684 24476-24483 NN denotes surface
T7685 24484-24488 CD denotes TLR4
T7686 24489-24498 NNS denotes complexes
T7687 24499-24501 VBZ denotes is
T7688 24502-24509 JJ denotes similar
T7689 24510-24517 IN denotes between
T7690 24518-24520 NNP denotes WT
T7691 24521-24524 CC denotes and
T7692 24525-24529 VB denotes cpdm
T7693 24530-24534 NNP denotes BMDC
T7694 24535-24545 VBG denotes suggesting
T7695 24546-24550 IN denotes that
T7696 24551-24560 JJ denotes defective
T7697 24561-24566 NN denotes NF-κB
T7698 24567-24577 NN denotes activation
T7699 24578-24580 VBZ denotes is
T7700 24581-24584 RB denotes not
T7701 24585-24586 DT denotes a
T7702 24587-24593 NN denotes result
T7703 24594-24596 IN denotes of
T7704 24597-24609 JJ denotes differential
T7705 24610-24613 NNP denotes TLR
T7706 24614-24624 NN denotes expression
T7707 24625-24627 IN denotes on
T7708 24628-24634 NN denotes target
T7709 24635-24640 NNS denotes cells
T7710 24640-24641 . denotes .
T7711 24642-24645 NNP denotes LPS
T7712 24646-24653 VBZ denotes engages
T7713 24654-24658 CD denotes TLR4
T7714 24659-24661 TO denotes to
T7715 24662-24670 VB denotes activate
T7716 24671-24686 JJ denotes MYD88-dependent
T7717 24687-24690 CC denotes and
T7718 24691-24705 JJ denotes TRIF-dependent
T7719 24706-24714 NNS denotes pathways
T7720 24714-24715 , denotes ,
T7721 24716-24723 IN denotes whereas
T7722 24724-24728 CD denotes TLR3
T7723 24729-24739 VBN denotes stimulated
T7724 24740-24742 IN denotes by
T7725 24743-24747 NN denotes poly
T7726 24748-24749 PRP denotes I
T7727 24749-24750 : denotes :
T7728 24750-24751 NNP denotes C
T7729 24752-24756 RB denotes only
T7730 24757-24765 VBZ denotes triggers
T7731 24766-24780 JJ denotes TRIF-dependent
T7732 24781-24790 VBG denotes signaling
T7733 24791-24792 NNP denotes [
T7734 24792-24794 CD denotes 53
T7735 24794-24795 NNP denotes ]
T7736 24795-24796 . denotes .
T7737 24797-24800 DT denotes The
T7738 24801-24810 JJ denotes defective
T7739 24811-24816 NN denotes NF-κB
T7740 24817-24827 NN denotes activation
T7741 24828-24830 IN denotes by
T7742 24831-24835 DT denotes both
T7743 24836-24843 NNS denotes stimuli
T7744 24844-24852 VBZ denotes suggests
T7745 24853-24857 IN denotes that
T7746 24858-24861 DT denotes the
T7747 24862-24869 NNP denotes Sharpin
T7748 24870-24878 NN denotes mutation
T7749 24879-24889 VBZ denotes interferes
T7750 24890-24894 IN denotes with
T7751 24895-24898 DT denotes the
T7752 24899-24906 NN denotes protein
T7753 24907-24915 NNS denotes adaptors
T7754 24916-24918 CC denotes or
T7755 24919-24926 NNS denotes kinases
T7756 24927-24933 VBN denotes shared
T7757 24934-24936 IN denotes by
T7758 24937-24941 DT denotes both
T7759 24942-24951 VBG denotes signaling
T7760 24952-24960 NNS denotes pathways
T7761 24960-24961 , denotes ,
T7762 24962-24966 JJ denotes such
T7763 24967-24969 IN denotes as
T7764 24970-24974 CD denotes RIP1
T7765 24975-24978 CC denotes and
T7766 24979-24984 CD denotes TRAF6
T7767 24985-24986 NNP denotes [
T7768 24986-24988 CD denotes 18
T7769 24988-24989 NNP denotes ]
T7770 24989-24990 . denotes .
T7771 24991-24997 JJ denotes Recent
T7772 24998-25005 NNS denotes studies
T7773 25006-25018 VBD denotes demonstrated
T7774 25019-25023 IN denotes that
T7775 25024-25031 NNP denotes SHARPIN
T7776 25032-25041 NNS denotes interacts
T7777 25042-25046 IN denotes with
T7778 25047-25051 NNP denotes HOIP
T7779 25052-25054 TO denotes to
T7780 25055-25059 VB denotes form
T7781 25060-25065 NNP denotes LUBAC
T7782 25066-25070 IN denotes that
T7783 25071-25077 VBZ denotes exerts
T7784 25078-25081 PRP$ denotes its
T7785 25082-25111 JJ denotes linear-ubiquitin-chain-ligase
T7786 25112-25120 NN denotes activity
T7787 25121-25123 IN denotes on
T7788 25124-25129 JJ denotes NF-κB
T7789 25130-25139 VBG denotes signaling
T7790 25140-25147 NNS denotes players
T7791 25148-25152 NNP denotes RIP1
T7792 25153-25156 CC denotes and
T7793 25157-25161 NNP denotes NEMO
T7794 25162-25163 NNP denotes [
T7795 25163-25164 CD denotes 7
T7796 25164-25165 NNP denotes ]
T7797 25165-25166 , denotes ,
T7798 25167-25169 DT denotes an
T7799 25170-25179 JJ denotes essential
T7800 25180-25184 NN denotes step
T7801 25185-25188 IN denotes for
T7802 25189-25195 JJ denotes intact
T7803 25196-25211 JJ denotes TNFα-stimulated
T7804 25212-25217 NN denotes NF-κB
T7805 25218-25228 NN denotes activation
T7806 25228-25229 . denotes .
T7807 25230-25233 DT denotes The
T7808 25234-25241 NNP denotes Sharpin
T7809 25242-25246 NN denotes null
T7810 25247-25255 NN denotes mutation
T7811 25256-25264 VBZ denotes disrupts
T7812 25265-25268 DT denotes the
T7813 25269-25283 NN denotes ubiquitylation
T7814 25284-25291 NN denotes process
T7815 25292-25295 CC denotes and
T7816 25296-25305 VBZ denotes abrogates
T7817 25306-25309 DT denotes the
T7818 25310-25322 JJ denotes TNFα-induced
T7819 25323-25328 NN denotes NF-κB
T7820 25329-25338 VBG denotes signaling
T7821 25339-25346 NN denotes pathway
T7822 25346-25347 . denotes .
T7823 25348-25353 IN denotes Since
T7824 25354-25358 NNP denotes TNFR
T7825 25359-25362 CC denotes and
T7826 25363-25366 NNP denotes TLR
T7827 25367-25376 RB denotes partially
T7828 25377-25382 VBP denotes share
T7829 25383-25388 PRP$ denotes their
T7830 25389-25399 JJ denotes downstream
T7831 25400-25409 VBG denotes signaling
T7832 25410-25418 NNS denotes cascades
T7833 25418-25419 , denotes ,
T7834 25420-25421 DT denotes a
T7835 25422-25429 JJ denotes similar
T7836 25430-25448 JJ denotes ubiquitin-mediated
T7837 25449-25459 NN denotes regulation
T7838 25460-25463 MD denotes may
T7839 25464-25468 VB denotes hold
T7840 25469-25473 JJ denotes true
T7841 25474-25477 IN denotes for
T7842 25478-25485 NNP denotes SHARPIN
T7843 25486-25488 IN denotes in
T7844 25489-25492 NNP denotes LPS
T7845 25492-25493 : denotes -
T7846 25494-25497 CC denotes and
T7847 25498-25502 RB denotes poly
T7848 25503-25504 PRP denotes I
T7849 25504-25505 : denotes :
T7850 25505-25514 JJ denotes C-induced
T7851 25515-25520 JJ denotes NF-κB
T7852 25521-25531 NN denotes activation
T7853 25531-25532 . denotes .
T7854 25533-25535 IN denotes In
T7855 25536-25543 NN denotes summary
T7856 25543-25544 , denotes ,
T7857 25545-25548 DT denotes the
T7858 25549-25556 JJ denotes present
T7859 25557-25562 NN denotes study
T7860 25563-25573 VBD denotes identified
T7861 25574-25576 DT denotes an
T7862 25577-25590 JJ denotes indispensible
T7863 25591-25595 NN denotes role
T7864 25596-25598 IN denotes of
T7865 25599-25606 NNP denotes SHARPIN
T7866 25607-25609 IN denotes in
T7867 25610-25613 DT denotes the
T7868 25614-25624 NN denotes production
T7869 25625-25627 IN denotes of
T7870 25628-25644 JJ denotes pro-inflammatory
T7871 25645-25654 NNS denotes mediators
T7872 25655-25658 CC denotes and
T7873 25659-25670 JJ denotes TLR-induced
T7874 25671-25676 NN denotes NF-κB
T7875 25677-25686 VBG denotes signaling
T7876 25686-25687 . denotes .
T7877 25688-25691 DT denotes The
T7878 25692-25700 VBN denotes impaired
T7879 25701-25716 NN denotes Th1-stimulating
T7880 25717-25724 NN denotes ability
T7881 25725-25727 IN denotes of
T7882 25728-25745 JJ denotes Sharpin-deficient
T7883 25746-25748 NNP denotes DC
T7884 25749-25752 MD denotes may
T7885 25753-25760 VB denotes account
T7886 25761-25764 IN denotes for
T7887 25765-25768 DT denotes the
T7888 25769-25781 JJ denotes Th2-dominant
T7889 25782-25794 JJ denotes inflammatory
T7890 25795-25804 NN denotes phenotype
T7891 25805-25807 IN denotes of
T7892 25808-25812 NN denotes cpdm
T7893 25813-25817 NNS denotes mice
T7894 25817-25818 . denotes .
T7895 25819-25822 DT denotes The
T7896 25823-25830 NN denotes balance
T7897 25831-25838 IN denotes between
T7898 25839-25842 CD denotes Th1
T7899 25843-25846 CC denotes and
T7900 25847-25850 CD denotes Th2
T7901 25851-25866 NN denotes differentiation
T7902 25867-25869 VBZ denotes is
T7903 25870-25878 JJ denotes critical
T7904 25879-25882 IN denotes for
T7905 25883-25889 JJ denotes immune
T7906 25890-25901 NN denotes homeostasis
T7907 25901-25902 . denotes .
T7908 25903-25904 DT denotes A
T7909 25905-25911 JJR denotes better
T7910 25912-25925 NN denotes understanding
T7911 25926-25928 IN denotes of
T7912 25929-25932 WRB denotes how
T7913 25933-25937 JJ denotes such
T7914 25938-25945 NN denotes balance
T7915 25946-25948 VBZ denotes is
T7916 25949-25959 VBN denotes maintained
T7917 25960-25964 MD denotes will
T7918 25965-25969 VB denotes help
T7919 25970-25976 NN denotes design
T7920 25977-25985 NN denotes cytokine
T7921 25986-25995 NN denotes treatment
T7922 25996-25999 IN denotes for
T7923 26000-26005 JJ denotes human
T7924 26006-26014 NNS denotes diseases
T7925 26015-26019 IN denotes with
T7926 26020-26030 JJ denotes Th2-biased
T7927 26031-26039 NN denotes cytokine
T7928 26040-26049 NN denotes secretion
T7929 26050-26057 JJ denotes similar
T7930 26058-26060 TO denotes to
T7931 26061-26064 DT denotes the
T7932 26065-26070 NN denotes mouse
T7933 26071-26075 NN denotes cpdm
T7934 26075-26076 , denotes ,
T7935 26077-26081 JJ denotes such
T7936 26082-26084 IN denotes as
T7937 26085-26094 NNS denotes allergies
T7938 26095-26098 CC denotes and
T7939 26099-26116 JJ denotes hypereosinophilic
T7940 26117-26126 NNS denotes syndromes
T7941 26126-26127 . denotes .
T9579 26152-26158 NNS denotes Ethics
T9580 26159-26168 NNP denotes Statement
T9581 26169-26172 NNP denotes All
T9582 26173-26178 NN denotes mouse
T9583 26179-26183 NN denotes work
T9584 26184-26187 VBD denotes was
T9585 26188-26195 VBN denotes carried
T9586 26196-26199 RP denotes out
T9587 26200-26202 IN denotes in
T9588 26203-26209 JJ denotes strict
T9589 26210-26220 NN denotes accordance
T9590 26221-26225 IN denotes with
T9591 26226-26235 NNS denotes protocols
T9592 26236-26244 VBN denotes approved
T9593 26245-26247 IN denotes by
T9594 26248-26251 DT denotes the
T9595 26252-26265 JJ denotes Institutional
T9596 26266-26272 NNP denotes Animal
T9597 26273-26277 NNP denotes Care
T9598 26278-26281 CC denotes and
T9599 26282-26285 NNP denotes Use
T9600 26286-26296 NNS denotes Committees
T9601 26296-26297 . denotes .
T9636 26299-26303 NNP denotes Mice
T9637 26304-26321 JJ denotes Specific-pathogen
T9638 26322-26326 JJ denotes free
T9639 26327-26335 NNS denotes colonies
T9640 26336-26338 IN denotes of
T9641 26339-26373 NN denotes C57BL/KaLawRij-Sharpincpdm/RijSunJ
T9642 26374-26375 -LRB- denotes (
T9643 26375-26377 NNP denotes JR
T9644 26377-26378 # denotes #
T9645 26378-26382 CD denotes 7599
T9646 26382-26383 -RRB- denotes )
T9647 26384-26387 CC denotes and
T9648 26388-26390 NNP denotes WT
T9649 26391-26395 NNS denotes mice
T9650 26396-26400 VBD denotes were
T9651 26401-26409 VBN denotes obtained
T9652 26410-26414 IN denotes from
T9653 26415-26418 DT denotes The
T9654 26419-26426 NNP denotes Jackson
T9655 26427-26437 NNP denotes Laboratory
T9656 26438-26439 -LRB- denotes (
T9657 26439-26442 NNP denotes Bar
T9658 26443-26449 NNP denotes Harbor
T9659 26449-26450 , denotes ,
T9660 26451-26453 NNP denotes ME
T9661 26453-26454 -RRB- denotes )
T9662 26455-26458 CC denotes and
T9663 26459-26463 VBD denotes were
T9664 26464-26474 VBN denotes maintained
T9665 26475-26477 IN denotes in
T9666 26478-26479 DT denotes a
T9667 26480-26487 NN denotes barrier
T9668 26488-26496 NN denotes facility
T9669 26496-26497 . denotes .
T9670 26498-26504 JJ denotes Normal
T9671 26505-26515 NN denotes littermate
T9672 26516-26524 NNS denotes controls
T9673 26525-26529 VBD denotes were
T9674 26530-26536 CC denotes either
T9675 26537-26538 VBD denotes +
T9676 26538-26539 CD denotes /
T9677 26539-26540 CD denotes +
T9678 26541-26543 CC denotes or
T9679 26544-26545 CD denotes +
T9680 26545-26546 CD denotes /
T9681 26546-26557 NNP denotes Sharpincpdm
T9682 26557-26558 . denotes .
T9683 26559-26564 DT denotes These
T9684 26565-26572 NN denotes control
T9685 26573-26580 NNS denotes animals
T9686 26581-26585 VBD denotes were
T9687 26586-26600 RB denotes phenotypically
T9688 26601-26618 JJ denotes indistinguishable
T9689 26619-26622 CC denotes and
T9690 26623-26626 VBP denotes are
T9691 26627-26635 VBN denotes referred
T9692 26636-26638 TO denotes to
T9693 26639-26641 IN denotes as
T9694 26642-26644 NNP denotes WT
T9695 26644-26645 . denotes .
T9696 26646-26657 JJ denotes Sex-matched
T9697 26658-26660 NNP denotes WT
T9698 26661-26664 CC denotes and
T9699 26665-26671 JJ denotes mutant
T9700 26672-26676 NNS denotes mice
T9701 26677-26681 VBD denotes were
T9702 26682-26686 VBN denotes used
T9703 26687-26689 IN denotes at
T9704 26690-26691 CD denotes 6
T9705 26692-26694 CD denotes 10
T9706 26695-26700 NNS denotes weeks
T9707 26701-26703 IN denotes of
T9708 26704-26707 NN denotes age
T9709 26707-26708 . denotes .
T9710 26709-26712 IN denotes For
T9711 26713-26717 DT denotes some
T9712 26718-26729 NNS denotes experiments
T9713 26729-26730 , denotes ,
T9714 26731-26735 NN denotes cpdm
T9715 26736-26740 NNS denotes mice
T9716 26741-26745 VBD denotes were
T9717 26746-26753 VBN denotes crossed
T9718 26754-26758 IN denotes with
T9719 26759-26769 JJ denotes transgenic
T9720 26770-26774 NNS denotes mice
T9721 26775-26779 IN denotes with
T9722 26780-26781 DT denotes a
T9723 26782-26791 JJ denotes bacterial
T9724 26792-26802 JJ denotes artificial
T9725 26803-26813 NN denotes chromosome
T9726 26814-26815 -LRB- denotes (
T9727 26815-26818 NNP denotes BAC
T9728 26818-26819 -RRB- denotes )
T9729 26820-26830 VBG denotes containing
T9730 26831-26834 DT denotes the
T9731 26835-26842 NNP denotes Sharpin
T9732 26843-26847 NN denotes gene
T9733 26848-26849 -LRB- denotes (
T9734 26849-26858 NNP denotes FVB/NJ-Tg
T9735 26858-26859 -LRB- denotes (
T9736 26859-26870 NNP denotes RP24-173I23
T9737 26870-26871 -RRB- denotes )
T9738 26871-26879 NNP denotes 1Sun/Sun
T9739 26879-26880 , denotes ,
T9740 26881-26883 NNP denotes JR
T9741 26883-26884 # denotes #
T9742 26884-26888 CD denotes 8279
T9743 26888-26889 -RRB- denotes )
T9744 26889-26890 . denotes .
T9745 26891-26896 DT denotes These
T9746 26897-26901 NNS denotes mice
T9747 26902-26906 VBD denotes were
T9748 26907-26918 VBN denotes backcrossed
T9749 26919-26923 IN denotes onto
T9750 26924-26927 DT denotes the
T9751 26928-26962 JJ denotes C57BL/KaLawRij-Sharpincpdm/RijSunJ
T9752 26963-26973 NN denotes background
T9753 26974-26977 CC denotes and
T9754 26978-26980 CD denotes N4
T9755 26981-26985 NNS denotes mice
T9756 26986-26990 VBD denotes were
T9757 26991-26995 VBN denotes used
T9758 26996-26998 IN denotes in
T9759 26999-27002 DT denotes the
T9760 27003-27014 NNS denotes experiments
T9761 27015-27023 VBD denotes reported
T9762 27024-27028 RB denotes here
T9763 27028-27029 . denotes .
T9764 27030-27033 DT denotes All
T9765 27034-27039 NN denotes mouse
T9766 27040-27044 NN denotes work
T9767 27045-27048 VBD denotes was
T9768 27049-27056 VBN denotes carried
T9769 27057-27060 RP denotes out
T9770 27061-27063 IN denotes in
T9771 27064-27070 JJ denotes strict
T9772 27071-27081 NN denotes accordance
T9773 27082-27086 IN denotes with
T9774 27087-27090 DT denotes the
T9775 27091-27099 VBN denotes approved
T9776 27100-27109 NNS denotes protocols
T9777 27110-27112 IN denotes by
T9778 27113-27116 DT denotes the
T9779 27117-27130 JJ denotes Institutional
T9780 27131-27137 NNP denotes Animal
T9781 27138-27142 NNP denotes Care
T9782 27143-27146 CC denotes and
T9783 27147-27150 NNP denotes Use
T9784 27151-27160 NNP denotes Committee
T9785 27160-27161 . denotes .
T9969 27163-27173 NNS denotes Constructs
T9970 27174-27177 CC denotes and
T9971 27178-27190 NN denotes transfection
T9972 27191-27194 DT denotes The
T9973 27195-27208 JJ denotes complementary
T9974 27209-27212 NN denotes DNA
T9975 27213-27214 -LRB- denotes (
T9976 27214-27218 NNP denotes cDNA
T9977 27218-27219 -RRB- denotes )
T9978 27220-27222 IN denotes of
T9979 27223-27226 DT denotes the
T9980 27227-27232 NN denotes mouse
T9981 27233-27240 NNP denotes Sharpin
T9982 27241-27245 NN denotes gene
T9983 27246-27249 VBD denotes was
T9984 27250-27256 VBN denotes cloned
T9985 27257-27260 CC denotes and
T9986 27261-27270 VBN denotes amplified
T9987 27271-27275 IN denotes from
T9988 27276-27282 NNP denotes RAW264
T9989 27282-27284 CD denotes .7
T9990 27285-27288 NNP denotes RNA
T9991 27289-27297 NNS denotes extracts
T9992 27297-27298 . denotes .
T9993 27299-27302 DT denotes The
T9994 27303-27309 NN denotes primer
T9995 27310-27319 NNS denotes sequences
T9996 27320-27324 VBD denotes were
T9997 27325-27332 RB denotes forward
T9998 27332-27333 , denotes ,
T9999 27334-27335 CD denotes 5
T10000 27335-27336 SYM denotes ′
T10001 27336-27337 : denotes -
T10002 27337-27339 NNP denotes CC
T10003 27340-27343 NNP denotes ATG
T10004 27344-27347 NNP denotes GCG
T10005 27348-27351 NNP denotes ATG
T10006 27352-27355 NNP denotes TCG
T10007 27356-27359 NNP denotes CCG
T10008 27360-27363 NNP denotes CCC
T10009 27364-27367 NNP denotes GCC
T10010 27368-27371 NNP denotes GGC
T10011 27372-27375 NNP denotes GGT
T10012 27375-27376 : denotes ;
T10013 27377-27384 VB denotes reverse
T10014 27384-27385 , denotes ,
T10015 27386-27387 CD denotes 5
T10016 27387-27388 SYM denotes ′
T10017 27388-27389 : denotes -
T10018 27390-27393 NNP denotes AAG
T10019 27394-27397 NNP denotes CTT
T10020 27398-27401 NNP denotes CTA
T10021 27402-27405 NNP denotes GGT
T10022 27406-27409 NNP denotes GGA
T10023 27410-27413 NNP denotes AGC
T10024 27414-27417 NNP denotes TGC
T10025 27418-27421 NNP denotes AGC
T10026 27422-27425 NNP denotes AAG
T10027 27426-27427 NNP denotes A
T10028 27427-27428 . denotes .
T10029 27429-27432 DT denotes The
T10030 27433-27440 NNP denotes Sharpin
T10031 27441-27445 NNP denotes cDNA
T10032 27446-27449 VBD denotes was
T10033 27450-27456 VBN denotes cloned
T10034 27457-27461 IN denotes into
T10035 27462-27472 NN denotes expression
T10036 27473-27479 NN denotes vector
T10037 27480-27491 NN denotes pFLAG-CMV-2
T10038 27491-27492 . denotes .
T10039 27493-27499 NNP denotes Murine
T10040 27500-27511 NNS denotes fibroblasts
T10041 27512-27515 CC denotes and
T10042 27516-27527 NNS denotes macrophages
T10043 27528-27532 VBD denotes were
T10044 27533-27537 VBD denotes were
T10045 27538-27542 VBN denotes used
T10046 27543-27545 TO denotes to
T10047 27546-27553 VB denotes express
T10048 27554-27565 JJ denotes recombinant
T10049 27566-27573 NNP denotes SHARPIN
T10050 27574-27581 NN denotes protein
T10051 27581-27582 . denotes .
T10052 27583-27588 NNS denotes Cells
T10053 27589-27590 -LRB- denotes (
T10054 27590-27591 CD denotes 2
T10055 27591-27592 CD denotes ×
T10056 27592-27595 CD denotes 104
T10057 27595-27596 -RRB- denotes )
T10058 27597-27601 VBD denotes were
T10059 27602-27608 VBN denotes seeded
T10060 27609-27611 IN denotes in
T10061 27612-27619 JJ denotes 96-well
T10062 27620-27627 VBN denotes treated
T10063 27628-27634 VBZ denotes plates
T10064 27635-27638 DT denotes the
T10065 27639-27642 NN denotes day
T10066 27643-27649 IN denotes before
T10067 27650-27662 NN denotes transfection
T10068 27662-27663 . denotes .
T10069 27664-27669 IN denotes After
T10070 27670-27679 JJ denotes overnight
T10071 27680-27690 NN denotes incubation
T10072 27690-27691 , denotes ,
T10073 27692-27695 CD denotes 200
T10074 27696-27698 NN denotes ng
T10075 27699-27712 NN denotes pFLAG-SHARPIN
T10076 27713-27721 NNS denotes plasmids
T10077 27722-27726 VBD denotes were
T10078 27727-27738 VBN denotes transfected
T10079 27739-27743 IN denotes with
T10080 27744-27747 CD denotes 0.5
T10081 27748-27750 NN denotes ul
T10082 27751-27764 NNP denotes Lipofectamine
T10083 27765-27769 CD denotes 2000
T10084 27769-27770 . denotes .
T10085 27771-27773 CD denotes 24
T10086 27774-27779 NNS denotes hours
T10087 27780-27785 RB denotes later
T10088 27785-27786 , denotes ,
T10089 27787-27792 NNS denotes cells
T10090 27793-27797 VBD denotes were
T10091 27798-27807 VBN denotes incubated
T10092 27808-27812 IN denotes with
T10093 27813-27818 JJ denotes fresh
T10094 27819-27826 NN denotes culture
T10095 27827-27833 NN denotes medium
T10096 27833-27834 . denotes .
T10097 27835-27840 IN denotes After
T10098 27841-27848 DT denotes another
T10099 27849-27851 CD denotes 24
T10100 27852-27857 NNS denotes hours
T10101 27857-27858 , denotes ,
T10102 27859-27864 NNS denotes cells
T10103 27865-27869 VBD denotes were
T10104 27870-27875 VBN denotes lysed
T10105 27876-27878 TO denotes to
T10106 27879-27886 VB denotes confirm
T10107 27887-27890 DT denotes the
T10108 27891-27903 NNP denotes FLAG-SHARPIN
T10109 27904-27914 NN denotes expression
T10110 27915-27917 IN denotes by
T10111 27918-27929 NNS denotes immunoblots
T10112 27930-27934 IN denotes with
T10113 27935-27944 JJ denotes anti-FLAG
T10114 27944-27945 . denotes .
T10115 27946-27951 NNS denotes Cells
T10116 27952-27956 IN denotes with
T10117 27957-27959 DT denotes no
T10118 27960-27972 NN denotes transfection
T10119 27973-27976 CC denotes and
T10120 27977-27988 VBN denotes transfected
T10121 27989-27993 IN denotes with
T10122 27994-27999 JJ denotes empty
T10123 28000-28006 NN denotes vector
T10124 28007-28018 NN denotes pFLAG-CMV-2
T10125 28019-28023 VBD denotes were
T10126 28024-28028 VBN denotes used
T10127 28029-28031 IN denotes as
T10128 28032-28040 JJ denotes negative
T10129 28041-28048 NN denotes control
T10130 28049-28051 IN denotes in
T10131 28052-28055 DT denotes all
T10132 28056-28068 NN denotes transfection
T10133 28069-28080 NNS denotes experiments
T10134 28080-28081 . denotes .
T10339 28083-28093 NNP denotes Generation
T10340 28094-28096 IN denotes of
T10341 28097-28101 NNP denotes BMDC
T10342 28102-28106 NNP denotes BMDC
T10343 28107-28111 VBD denotes were
T10344 28112-28121 VBN denotes developed
T10345 28122-28124 IN denotes as
T10346 28125-28135 RB denotes previously
T10347 28136-28144 VBN denotes reported
T10348 28145-28146 NNP denotes [
T10349 28146-28148 CD denotes 54
T10350 28148-28149 NNP denotes ]
T10351 28149-28150 , denotes ,
T10352 28151-28152 NNP denotes [
T10353 28152-28154 CD denotes 55
T10354 28154-28155 NNP denotes ]
T10355 28155-28156 . denotes .
T10356 28157-28162 NNS denotes Cells
T10357 28163-28167 VBD denotes were
T10358 28168-28177 VBN denotes collected
T10359 28178-28183 IN denotes after
T10360 28184-28186 CD denotes 10
T10361 28187-28189 CD denotes 12
T10362 28190-28194 NNS denotes days
T10363 28195-28197 IN denotes of
T10364 28198-28205 NN denotes culture
T10365 28205-28206 . denotes .
T10366 28207-28210 DT denotes The
T10367 28211-28215 NN denotes cell
T10368 28216-28221 NN denotes yield
T10369 28222-28225 VBD denotes was
T10370 28226-28227 CD denotes 2
T10371 28228-28229 CD denotes 3
T10372 28229-28230 CD denotes ×
T10373 28230-28233 CD denotes 107
T10374 28234-28245 NN denotes cells/mouse
T10375 28246-28250 IN denotes with
T10376 28251-28253 CD denotes 80
T10377 28254-28256 CD denotes 95
T10378 28256-28257 NN denotes %
T10379 28258-28262 NNP denotes BMDC
T10380 28262-28263 . denotes .
T10432 28265-28274 NNP denotes Phenotype
T10433 28275-28277 IN denotes of
T10434 28278-28282 NNP denotes BMDC
T10435 28283-28286 CC denotes and
T10436 28287-28294 JJ denotes splenic
T10437 28295-28297 NNP denotes DC
T10438 28298-28305 VBZ denotes subsets
T10439 28306-28309 DT denotes The
T10440 28310-28319 NN denotes phenotype
T10441 28320-28322 IN denotes of
T10442 28323-28327 NNP denotes BMDC
T10443 28328-28331 VBD denotes was
T10444 28332-28342 VBN denotes determined
T10445 28343-28349 IN denotes before
T10446 28350-28353 CC denotes and
T10447 28354-28359 IN denotes after
T10448 28360-28362 CD denotes 24
T10449 28363-28367 NN denotes hour
T10450 28368-28375 NN denotes culture
T10451 28376-28378 IN denotes in
T10452 28379-28382 DT denotes the
T10453 28383-28391 NN denotes presence
T10454 28392-28394 IN denotes of
T10455 28395-28398 CD denotes 100
T10456 28399-28404 NNP denotes ng/mL
T10457 28405-28408 NNP denotes LPS
T10458 28408-28409 . denotes .
T10459 28410-28413 DT denotes The
T10460 28414-28419 NNS denotes cells
T10461 28420-28424 VBD denotes were
T10462 28425-28434 VBN denotes incubated
T10463 28435-28437 IN denotes in
T10464 28438-28441 NNP denotes PBS
T10465 28442-28446 IN denotes with
T10466 28447-28450 CD denotes 0.1
T10467 28450-28451 NN denotes %
T10468 28452-28456 JJ denotes NaN3
T10469 28456-28457 , denotes ,
T10470 28458-28459 CD denotes 1
T10471 28459-28460 NN denotes %
T10472 28461-28464 NNP denotes BSA
T10473 28465-28468 CC denotes and
T10474 28469-28471 CD denotes 10
T10475 28471-28472 NN denotes %
T10476 28473-28479 JJ denotes normal
T10477 28480-28486 NN denotes rabbit
T10478 28487-28492 NN denotes serum
T10479 28493-28496 IN denotes for
T10480 28497-28499 CD denotes 20
T10481 28500-28507 NNS denotes minutes
T10482 28508-28510 IN denotes on
T10483 28511-28514 NN denotes ice
T10484 28514-28515 , denotes ,
T10485 28516-28522 VBD denotes washed
T10486 28523-28526 CC denotes and
T10487 28527-28536 VBD denotes incubated
T10488 28537-28540 IN denotes for
T10489 28541-28543 CD denotes 30
T10490 28544-28551 NNS denotes minutes
T10491 28552-28554 IN denotes on
T10492 28555-28558 NN denotes ice
T10493 28559-28563 IN denotes with
T10494 28564-28568 NNP denotes Alex
T10495 28569-28582 VBD denotes Fluor-labeled
T10496 28583-28593 JJ denotes anti-CD11c
T10497 28594-28595 -LRB- denotes (
T10498 28595-28603 NNP denotes MCD11C20
T10499 28603-28604 , denotes ,
T10500 28605-28611 NNP denotes CALTAG
T10501 28611-28612 -RRB- denotes )
T10502 28613-28615 IN denotes in
T10503 28616-28627 NN denotes combination
T10504 28628-28632 IN denotes with
T10505 28633-28635 NNP denotes PE
T10506 28635-28636 : denotes -
T10507 28637-28646 NN denotes anti-CD40
T10508 28647-28648 -LRB- denotes (
T10509 28648-28652 CD denotes 3/23
T10510 28652-28653 , denotes ,
T10511 28654-28656 NNP denotes BD
T10512 28657-28668 NNP denotes Biosciences
T10513 28668-28669 -RRB- denotes )
T10514 28669-28670 , denotes ,
T10515 28671-28683 NN denotes PE-anti-CD80
T10516 28684-28685 -LRB- denotes (
T10517 28685-28692 NNP denotes 16-10A1
T10518 28692-28693 , denotes ,
T10519 28694-28705 NNP denotes eBioscience
T10520 28705-28706 -RRB- denotes )
T10521 28706-28707 , denotes ,
T10522 28708-28720 NNP denotes PE-anti-CD86
T10523 28721-28722 -LRB- denotes (
T10524 28722-28725 NNP denotes P03
T10525 28725-28727 CD denotes .1
T10526 28727-28728 , denotes ,
T10527 28729-28740 NNP denotes eBioscience
T10528 28740-28741 -RRB- denotes )
T10529 28741-28742 , denotes ,
T10530 28743-28755 NNP denotes PE-anti-CD14
T10531 28756-28757 -LRB- denotes (
T10532 28757-28760 NNP denotes Sa2
T10533 28760-28762 CD denotes .8
T10534 28762-28763 , denotes ,
T10535 28764-28775 NNP denotes eBioscience
T10536 28775-28776 -RRB- denotes )
T10537 28777-28780 CC denotes and
T10538 28781-28793 VBD denotes biotinylated
T10539 28794-28803 JJ denotes anti-TLR4
T10540 28804-28805 -LRB- denotes (
T10541 28805-28814 NNP denotes BioLegend
T10542 28814-28815 -RRB- denotes )
T10543 28815-28816 . denotes .
T10544 28817-28820 DT denotes The
T10545 28821-28826 NNS denotes cells
T10546 28827-28836 VBN denotes incubated
T10547 28837-28841 IN denotes with
T10548 28842-28854 JJ denotes biotinylated
T10549 28855-28864 JJ denotes anti-TLR4
T10550 28864-28865 NN denotes /
T10551 28865-28869 NN denotes MD-2
T10552 28870-28874 VBD denotes were
T10553 28875-28881 VBN denotes washed
T10554 28882-28887 RB denotes twice
T10555 28888-28891 CC denotes and
T10556 28892-28901 VBN denotes incubated
T10557 28902-28905 IN denotes for
T10558 28906-28908 CD denotes 30
T10559 28909-28916 NNS denotes minutes
T10560 28917-28919 IN denotes on
T10561 28920-28923 NN denotes ice
T10562 28924-28928 IN denotes with
T10563 28929-28938 NNP denotes avidin-PE
T10564 28938-28939 . denotes .
T10565 28940-28942 TO denotes To
T10566 28943-28950 VB denotes isolate
T10567 28951-28958 JJ denotes splenic
T10568 28959-28961 NNP denotes DC
T10569 28961-28962 , denotes ,
T10570 28963-28970 NNS denotes spleens
T10571 28971-28975 VBD denotes were
T10572 28976-28987 JJ denotes collagenase
T10573 28988-28996 VBN denotes digested
T10574 28997-29000 CC denotes and
T10575 29001-29008 JJ denotes subject
T10576 29009-29011 TO denotes to
T10577 29012-29019 NNP denotes Percoll
T10578 29020-29028 NN denotes gradient
T10579 29029-29043 NN denotes centrifugation
T10580 29043-29044 . denotes .
T10581 29045-29048 DT denotes The
T10582 29049-29054 NNS denotes bands
T10583 29055-29057 IN denotes at
T10584 29058-29061 DT denotes the
T10585 29062-29064 CD denotes 35
T10586 29065-29067 CD denotes 55
T10587 29067-29068 NN denotes %
T10588 29069-29078 NN denotes interface
T10589 29079-29083 VBD denotes were
T10590 29084-29093 VBN denotes collected
T10591 29094-29096 TO denotes to
T10592 29097-29102 VB denotes stain
T10593 29103-29107 IN denotes with
T10594 29108-29121 NNP denotes PE-anti-CD11c
T10595 29122-29123 -LRB- denotes (
T10596 29123-29126 NNP denotes HL3
T10597 29126-29127 , denotes ,
T10598 29128-29137 NNP denotes BioLegend
T10599 29137-29138 -RRB- denotes )
T10600 29138-29139 , denotes ,
T10601 29140-29155 NNP denotes APC-anti-PDCA-1
T10602 29156-29157 -LRB- denotes (
T10603 29157-29160 CD denotes 927
T10604 29160-29161 , denotes ,
T10605 29162-29171 NNP denotes BioLegend
T10606 29171-29172 -RRB- denotes )
T10607 29173-29176 CC denotes and
T10608 29177-29191 NNP denotes FITC-anti-CD8α
T10609 29192-29193 -LRB- denotes (
T10610 29193-29195 CD denotes 53
T10611 29196-29199 CD denotes 6.7
T10612 29199-29200 , denotes ,
T10613 29201-29203 NNP denotes BD
T10614 29204-29214 NNP denotes BioScience
T10615 29214-29215 -RRB- denotes )
T10616 29215-29216 . denotes .
T10617 29217-29220 DT denotes The
T10618 29221-29226 NNS denotes cells
T10619 29227-29231 VBD denotes were
T10620 29232-29238 VBN denotes washed
T10621 29239-29244 RB denotes twice
T10622 29244-29245 , denotes ,
T10623 29246-29251 VBN denotes fixed
T10624 29252-29254 IN denotes in
T10625 29255-29256 CD denotes 2
T10626 29256-29257 NN denotes %
T10627 29258-29274 NN denotes paraformaldehyde
T10628 29274-29275 , denotes ,
T10629 29276-29279 CC denotes and
T10630 29280-29286 VBD denotes stored
T10631 29287-29289 IN denotes at
T10632 29290-29291 CD denotes 4
T10633 29291-29292 CD denotes °
T10634 29292-29293 NNP denotes C
T10635 29294-29299 IN denotes until
T10636 29300-29308 NN denotes analysis
T10637 29308-29309 . denotes .
T10638 29310-29314 NN denotes Flow
T10639 29315-29324 NN denotes cytometry
T10640 29325-29328 VBD denotes was
T10641 29329-29338 VBN denotes performed
T10642 29339-29341 IN denotes in
T10643 29342-29344 DT denotes an
T10644 29345-29350 NNP denotes Excel
T10645 29351-29352 -LRB- denotes (
T10646 29352-29359 NNP denotes Coulter
T10647 29359-29360 -RRB- denotes )
T10648 29361-29371 NN denotes instrument
T10649 29371-29372 . denotes .
T10650 29373-29377 NNP denotes Dead
T10651 29378-29383 NNS denotes cells
T10652 29384-29388 VBD denotes were
T10653 29389-29396 VBN denotes omitted
T10654 29397-29401 IN denotes from
T10655 29402-29405 DT denotes the
T10656 29406-29414 NN denotes analysis
T10657 29415-29417 IN denotes by
T10658 29418-29424 VBG denotes gating
T10659 29425-29427 IN denotes on
T10660 29428-29435 RB denotes forward
T10661 29436-29439 CC denotes and
T10662 29440-29442 CD denotes 90
T10663 29442-29443 NN denotes °
T10664 29444-29449 JJ denotes light
T10665 29450-29457 NN denotes scatter
T10666 29457-29458 , denotes ,
T10667 29459-29462 CC denotes and
T10668 29463-29469 CD denotes 10,000
T10669 29470-29475 NNS denotes cells
T10670 29476-29480 VBD denotes were
T10671 29481-29489 VBN denotes analyzed
T10672 29490-29492 IN denotes by
T10673 29493-29499 NNP denotes FlowJo
T10674 29500-29508 NN denotes software
T10675 29508-29509 . denotes .
T10967 29511-29517 NNP denotes BMDC-T
T10968 29518-29522 NN denotes cell
T10969 29523-29525 IN denotes in
T10970 29526-29531 NN denotes vitro
T10971 29532-29543 NN denotes interaction
T10972 29544-29546 NNP denotes WT
T10973 29547-29550 CC denotes and
T10974 29551-29555 VB denotes cpdm
T10975 29556-29560 NNP denotes BMDC
T10976 29561-29562 -LRB- denotes (
T10977 29562-29563 CD denotes 5
T10978 29563-29564 CD denotes ×
T10979 29564-29567 CD denotes 104
T10980 29567-29568 -RRB- denotes )
T10981 29569-29573 VBD denotes were
T10982 29574-29584 VBN denotes stimulated
T10983 29585-29589 IN denotes with
T10984 29590-29596 NN denotes medium
T10985 29596-29597 , denotes ,
T10986 29598-29599 CD denotes 1
T10987 29600-29602 NN denotes µg
T10988 29602-29603 NN denotes /
T10989 29603-29605 NNP denotes mL
T10990 29606-29609 NNP denotes LPS
T10991 29609-29610 , denotes ,
T10992 29611-29613 CD denotes 25
T10993 29614-29616 NN denotes µg
T10994 29616-29617 NN denotes /
T10995 29617-29619 NNP denotes mL
T10996 29620-29623 CC denotes and
T10997 29624-29625 CD denotes 5
T10998 29626-29628 NN denotes µg
T10999 29628-29629 NN denotes /
T11000 29629-29631 NNP denotes mL
T11001 29632-29639 NNP denotes Pam3CYS
T11002 29639-29640 . denotes .
T11003 29641-29643 CD denotes 24
T11004 29644-29649 NNS denotes hours
T11005 29650-29655 RB denotes later
T11006 29655-29656 , denotes ,
T11007 29657-29662 NNS denotes cells
T11008 29663-29667 VBD denotes were
T11009 29668-29677 VBN denotes collected
T11010 29678-29681 CC denotes and
T11011 29682-29688 VBN denotes washed
T11012 29689-29693 IN denotes with
T11013 29694-29697 NNP denotes PBS
T11014 29697-29698 . denotes .
T11015 29699-29709 JJ denotes Allogeneic
T11016 29710-29715 NN denotes naïve
T11017 29716-29719 NNP denotes CD4
T11018 29719-29720 NNP denotes +
T11019 29721-29722 NNP denotes T
T11020 29723-29728 NNS denotes cells
T11021 29729-29733 VBD denotes were
T11022 29734-29742 VBN denotes isolated
T11023 29743-29747 IN denotes from
T11024 29748-29755 NNS denotes spleens
T11025 29756-29758 IN denotes of
T11026 29759-29765 NNP denotes BALB/c
T11027 29766-29770 NNS denotes mice
T11028 29771-29773 IN denotes by
T11029 29774-29782 JJ denotes negative
T11030 29783-29792 NN denotes selection
T11031 29793-29796 NN denotes kit
T11032 29797-29798 -LRB- denotes (
T11033 29798-29808 NNP denotes Invitrogen
T11034 29808-29809 -RRB- denotes )
T11035 29810-29813 CC denotes and
T11036 29814-29818 VBD denotes were
T11037 29819-29823 RB denotes then
T11038 29824-29829 VBN denotes added
T11039 29830-29832 IN denotes at
T11040 29833-29836 CD denotes 2.5
T11041 29836-29837 CD denotes ×
T11042 29837-29840 CD denotes 105
T11043 29841-29844 CC denotes and
T11044 29845-29856 JJ denotes co-cultured
T11045 29857-29861 IN denotes with
T11046 29862-29871 VBN denotes activated
T11047 29872-29876 NNP denotes BMDC
T11048 29876-29877 . denotes .
T11049 29878-29883 IN denotes After
T11050 29884-29885 CD denotes 5
T11051 29886-29890 NNS denotes days
T11052 29890-29891 , denotes ,
T11053 29892-29903 NN denotes supernatant
T11054 29904-29907 VBD denotes was
T11055 29908-29917 VBN denotes collected
T11056 29918-29921 CC denotes and
T11057 29922-29925 DT denotes the
T11058 29926-29935 NN denotes secretion
T11059 29936-29938 IN denotes of
T11060 29939-29943 NNP denotes IFNγ
T11061 29943-29944 , denotes ,
T11062 29945-29948 NNP denotes IL4
T11063 29948-29949 , denotes ,
T11064 29950-29953 NNP denotes IL2
T11065 29953-29954 , denotes ,
T11066 29955-29958 CC denotes and
T11067 29959-29964 NNP denotes IL17A
T11068 29965-29973 VBN denotes measured
T11069 29974-29976 IN denotes by
T11070 29977-29982 NNP denotes ELISA
T11071 29982-29983 . denotes .
T11072 29984-29992 JJ denotes Negative
T11073 29993-30001 NNS denotes controls
T11074 30002-30005 VBP denotes are
T11075 30006-30007 CD denotes 1
T11076 30007-30008 -RRB- denotes )
T11077 30009-30019 VBD denotes stimulated
T11078 30020-30024 NNP denotes BMDC
T11079 30025-30032 IN denotes without
T11080 30033-30043 NN denotes co-culture
T11081 30044-30048 IN denotes with
T11082 30049-30059 JJ denotes allogeneic
T11083 30060-30063 NNP denotes CD4
T11084 30063-30064 CD denotes +
T11085 30065-30066 NNP denotes T
T11086 30067-30072 NNS denotes cells
T11087 30072-30073 : denotes ;
T11088 30074-30075 LS denotes 2
T11089 30075-30076 -RRB- denotes )
T11090 30077-30087 JJ denotes allogeneic
T11091 30088-30091 NNP denotes CD4
T11092 30091-30092 CD denotes +
T11093 30093-30094 NNP denotes T
T11094 30095-30100 NNS denotes cells
T11095 30101-30108 IN denotes without
T11096 30109-30119 NN denotes co-culture
T11097 30120-30124 IN denotes with
T11098 30125-30135 VBN denotes stimulated
T11099 30136-30140 NNP denotes BMDC
T11100 30140-30141 . denotes .
T11101 30142-30146 DT denotes Both
T11102 30147-30155 JJ denotes negative
T11103 30156-30164 NNS denotes controls
T11104 30165-30169 VBP denotes show
T11105 30170-30172 DT denotes no
T11106 30173-30183 NN denotes production
T11107 30184-30186 IN denotes of
T11108 30187-30201 JJ denotes aforementioned
T11109 30202-30211 NNS denotes cytokines
T11110 30211-30212 . denotes .
T11358 30214-30217 NNP denotes RNA
T11359 30218-30228 NN denotes expression
T11360 30229-30231 IN denotes by
T11361 30232-30236 NNP denotes BMDC
T11362 30237-30241 NNP denotes BMDC
T11363 30242-30246 VBD denotes were
T11364 30247-30255 VBN denotes cultured
T11365 30256-30258 IN denotes at
T11366 30259-30262 CD denotes 106
T11367 30263-30271 NN denotes cells/mL
T11368 30272-30274 IN denotes in
T11369 30275-30277 CD denotes 10
T11370 30278-30280 NN denotes mL
T11371 30281-30283 IN denotes of
T11372 30284-30293 NN denotes RPMI-1640
T11373 30294-30302 JJ denotes complete
T11374 30303-30309 NN denotes medium
T11375 30310-30312 IN denotes in
T11376 30313-30316 DT denotes the
T11377 30317-30325 NN denotes presence
T11378 30326-30328 CC denotes or
T11379 30329-30336 NN denotes absence
T11380 30337-30339 IN denotes of
T11381 30340-30343 CD denotes 100
T11382 30344-30349 NNP denotes ng/mL
T11383 30350-30353 NNP denotes LPS
T11384 30354-30356 CC denotes or
T11385 30357-30359 CD denotes 25
T11386 30360-30362 NN denotes µg
T11387 30362-30363 CD denotes /
T11388 30363-30365 NNP denotes mL
T11389 30366-30370 NN denotes poly
T11390 30371-30372 PRP denotes I
T11391 30372-30373 : denotes :
T11392 30373-30374 NNP denotes C
T11393 30374-30375 . denotes .
T11394 30376-30381 IN denotes After
T11395 30382-30383 CD denotes 1
T11396 30384-30387 CC denotes and
T11397 30388-30389 CD denotes 2
T11398 30390-30395 NNS denotes hours
T11399 30395-30396 , denotes ,
T11400 30397-30400 NNP denotes RNA
T11401 30401-30404 VBD denotes was
T11402 30405-30413 VBN denotes isolated
T11403 30414-30418 IN denotes with
T11404 30419-30430 JJ denotes TRI-reagent
T11405 30431-30432 -LRB- denotes (
T11406 30432-30437 NNP denotes Sigma
T11407 30437-30438 -RRB- denotes )
T11408 30439-30448 VBG denotes according
T11409 30449-30451 TO denotes to
T11410 30452-30455 DT denotes the
T11411 30456-30468 NN denotes manufacturer
T11412 30468-30470 POS denotes 's
T11413 30471-30483 NNS denotes instructions
T11414 30483-30484 . denotes .
T11415 30485-30488 DT denotes The
T11416 30489-30499 NN denotes expression
T11417 30500-30502 IN denotes of
T11418 30503-30506 NNP denotes Il6
T11419 30506-30507 , denotes ,
T11420 30508-30515 NNP denotes Il12p40
T11421 30515-30516 , denotes ,
T11422 30517-30522 NNP denotes Gmcsf
T11423 30522-30523 , denotes ,
T11424 30524-30527 CC denotes and
T11425 30528-30535 NNP denotes Sharpin
T11426 30536-30540 NNP denotes mRNA
T11427 30541-30544 VBD denotes was
T11428 30545-30555 VBN denotes determined
T11429 30556-30558 IN denotes by
T11430 30559-30566 NNP denotes qRT-PCR
T11431 30566-30567 . denotes .
T11432 30568-30575 NNS denotes Primers
T11433 30576-30579 CC denotes and
T11434 30580-30586 NNS denotes probes
T11435 30587-30591 VBD denotes were
T11436 30592-30601 VBN denotes purchased
T11437 30602-30606 IN denotes from
T11438 30607-30614 NNP denotes Applied
T11439 30615-30625 NNPS denotes Biosystems
T11440 30625-30626 . denotes .
T11441 30627-30634 VB denotes Reverse
T11442 30635-30648 NN denotes transcription
T11443 30649-30652 VBD denotes was
T11444 30653-30662 VBN denotes performed
T11445 30663-30665 IN denotes at
T11446 30666-30668 CD denotes 42
T11447 30668-30669 CD denotes °
T11448 30669-30670 NNP denotes C
T11449 30671-30674 IN denotes for
T11450 30675-30677 CD denotes 60
T11451 30678-30685 NNS denotes minutes
T11452 30686-30690 IN denotes with
T11453 30691-30694 DT denotes the
T11454 30695-30700 JJ denotes final
T11455 30701-30713 NN denotes denaturation
T11456 30714-30718 NN denotes step
T11457 30719-30721 IN denotes at
T11458 30722-30724 CD denotes 90
T11459 30724-30725 CD denotes °
T11460 30725-30726 NNP denotes C
T11461 30727-30730 IN denotes for
T11462 30731-30732 CD denotes 5
T11463 30733-30740 NNS denotes minutes
T11464 30741-30743 IN denotes in
T11465 30744-30746 CD denotes 30
T11466 30747-30749 NN denotes µl
T11467 30750-30760 VBG denotes containing
T11468 30761-30764 CD denotes 0.5
T11469 30765-30767 NN denotes µg
T11470 30768-30770 IN denotes of
T11471 30771-30776 JJ denotes total
T11472 30777-30780 NNP denotes RNA
T11473 30780-30781 . denotes .
T11474 30782-30787 NNS denotes dNTPs
T11475 30787-30788 , denotes ,
T11476 30789-30794 NN denotes oligo
T11477 30794-30795 -LRB- denotes (
T11478 30795-30797 NN denotes dT
T11479 30797-30798 -RRB- denotes )
T11480 30798-30800 CD denotes 15
T11481 30801-30807 NN denotes primer
T11482 30807-30808 , denotes ,
T11483 30809-30820 JJ denotes recombinant
T11484 30821-30827 NNP denotes RNasin
T11485 30828-30840 NNP denotes Ribonuclease
T11486 30841-30850 NNP denotes Inhibitor
T11487 30850-30851 , denotes ,
T11488 30852-30855 CC denotes and
T11489 30856-30861 NNP denotes M-MLV
T11490 30862-30869 VB denotes Reverse
T11491 30870-30883 NNP denotes Transcriptase
T11492 30884-30885 -LRB- denotes (
T11493 30885-30888 DT denotes all
T11494 30889-30893 IN denotes from
T11495 30894-30901 NNP denotes Promega
T11496 30901-30902 , denotes ,
T11497 30903-30910 NNP denotes Madison
T11498 30910-30911 , denotes ,
T11499 30912-30914 NNP denotes WI
T11500 30914-30915 -RRB- denotes )
T11501 30916-30920 VBD denotes were
T11502 30921-30925 VBN denotes used
T11503 30926-30935 VBG denotes according
T11504 30936-30938 TO denotes to
T11505 30939-30942 DT denotes the
T11506 30943-30955 NN denotes manufacturer
T11507 30955-30957 POS denotes 's
T11508 30958-30969 NN denotes instruction
T11509 30969-30970 . denotes .
T11510 30971-30978 VB denotes Reverse
T11511 30979-30992 NN denotes transcription
T11512 30993-30996 VBD denotes was
T11513 30997-31001 VBN denotes done
T11514 31002-31004 IN denotes in
T11515 31005-31006 DT denotes a
T11516 31007-31014 NN denotes PTC-200
T11517 31015-31022 NNP denotes Peltier
T11518 31023-31030 JJ denotes Thermal
T11519 31031-31037 NNP denotes Cycler
T11520 31038-31039 -LRB- denotes (
T11521 31039-31041 NNP denotes MJ
T11522 31042-31050 NNP denotes Research
T11523 31050-31051 , denotes ,
T11524 31052-31061 NNP denotes Watertown
T11525 31061-31062 , denotes ,
T11526 31063-31065 NNP denotes MA
T11527 31065-31066 -RRB- denotes )
T11528 31066-31067 . denotes .
T11529 31068-31075 NN denotes qRT-PCR
T11530 31076-31079 VBD denotes was
T11531 31080-31089 VBN denotes performed
T11532 31090-31092 IN denotes in
T11533 31093-31096 NNP denotes ABI
T11534 31097-31102 NNP denotes Prism
T11535 31103-31107 CD denotes 7700
T11536 31108-31116 NNP denotes Sequence
T11537 31117-31126 NNP denotes Detection
T11538 31127-31133 NNP denotes System
T11539 31134-31138 IN denotes with
T11540 31139-31145 NNP denotes TaqMan
T11541 31145-31146 NNP denotes ®
T11542 31147-31151 NNP denotes Gene
T11543 31152-31162 NNP denotes Expression
T11544 31163-31169 NNP denotes Assays
T11545 31170-31171 -LRB- denotes (
T11546 31171-31178 NNP denotes Applied
T11547 31179-31189 NNPS denotes Biosystems
T11548 31189-31190 , denotes ,
T11549 31191-31197 NNP denotes Foster
T11550 31198-31202 NNP denotes City
T11551 31202-31203 , denotes ,
T11552 31204-31206 NNP denotes CA
T11553 31206-31207 -RRB- denotes )
T11554 31208-31211 IN denotes for
T11555 31212-31217 NN denotes mouse
T11556 31218-31222 NNP denotes Actb
T11557 31222-31223 , denotes ,
T11558 31224-31227 NNP denotes IL6
T11559 31227-31228 , denotes ,
T11560 31229-31236 NNP denotes IL12p40
T11561 31236-31237 , denotes ,
T11562 31238-31242 NNP denotes Ifnβ
T11563 31242-31243 , denotes ,
T11564 31244-31249 NNP denotes Gmcsf
T11565 31249-31250 , denotes ,
T11566 31251-31254 CC denotes and
T11567 31255-31262 NNP denotes Sharpin
T11568 31263-31272 VBG denotes according
T11569 31273-31275 TO denotes to
T11570 31276-31279 DT denotes the
T11571 31280-31292 NN denotes manufacturer
T11572 31292-31294 POS denotes 's
T11573 31295-31303 NN denotes protocol
T11574 31303-31304 . denotes .
T11575 31305-31308 DT denotes The
T11576 31309-31319 JJ denotes endogenous
T11577 31320-31328 NN denotes standard
T11578 31329-31332 IN denotes for
T11579 31333-31346 NN denotes normalization
T11580 31347-31349 IN denotes of
T11581 31350-31353 DT denotes the
T11582 31354-31360 NN denotes target
T11583 31361-31365 NN denotes gene
T11584 31366-31369 VBD denotes was
T11585 31370-31377 JJ denotes β-actin
T11586 31377-31378 . denotes .
T11587 31379-31387 JJ denotes Relative
T11588 31388-31392 NN denotes gene
T11589 31393-31403 NN denotes expression
T11590 31404-31407 VBD denotes was
T11591 31408-31418 VBN denotes calculated
T11592 31419-31424 VBG denotes using
T11593 31425-31428 DT denotes the
T11594 31429-31430 CD denotes 2
T11595 31430-31431 NN denotes −
T11596 31431-31435 NNP denotes ΔΔCt
T11597 31436-31442 NN denotes method
T11598 31443-31444 NNP denotes [
T11599 31444-31446 CD denotes 56
T11600 31446-31447 NNP denotes ]
T11601 31447-31448 . denotes .
T11925 31450-31452 IN denotes In
T11926 31453-31458 NN denotes vitro
T11927 31459-31467 NN denotes cytokine
T11928 31468-31477 NN denotes secretion
T11929 31478-31480 IN denotes by
T11930 31481-31485 NNP denotes BMDC
T11931 31486-31489 NNP denotes The
T11932 31490-31494 NNP denotes BMDC
T11933 31495-31499 VBD denotes were
T11934 31500-31508 VBN denotes cultured
T11935 31509-31511 IN denotes in
T11936 31512-31522 NN denotes triplicate
T11937 31523-31528 NNS denotes wells
T11938 31529-31531 IN denotes of
T11939 31532-31539 JJ denotes 24-well
T11940 31540-31542 CC denotes or
T11941 31543-31550 JJ denotes 96-well
T11942 31551-31557 NNS denotes plates
T11943 31558-31560 IN denotes at
T11944 31561-31564 CD denotes 106
T11945 31565-31567 CC denotes or
T11946 31568-31571 CD denotes 105
T11947 31572-31580 NNP denotes cells/mL
T11948 31580-31581 , denotes ,
T11949 31582-31594 RB denotes respectively
T11950 31594-31595 , denotes ,
T11951 31596-31598 IN denotes in
T11952 31599-31608 NN denotes RPMI-1640
T11953 31609-31617 JJ denotes complete
T11954 31618-31624 NN denotes medium
T11955 31624-31625 . denotes .
T11956 31626-31629 DT denotes The
T11957 31630-31635 NNS denotes cells
T11958 31636-31640 VBD denotes were
T11959 31641-31647 VBN denotes washed
T11960 31648-31651 CC denotes and
T11961 31652-31662 VBN denotes stimulated
T11962 31663-31667 IN denotes with
T11963 31668-31671 CD denotes 100
T11964 31672-31677 NNP denotes ng/mL
T11965 31678-31681 NNP denotes LPS
T11966 31682-31684 CC denotes or
T11967 31685-31687 CD denotes 25
T11968 31688-31690 NN denotes µg
T11969 31690-31691 CD denotes /
T11970 31691-31693 NNP denotes mL
T11971 31694-31698 NN denotes poly
T11972 31699-31700 PRP denotes I
T11973 31700-31701 : denotes :
T11974 31701-31702 NNP denotes C
T11975 31702-31703 . denotes .
T11976 31704-31709 IN denotes After
T11977 31710-31712 CD denotes 24
T11978 31713-31718 NNS denotes hours
T11979 31718-31719 , denotes ,
T11980 31720-31732 NNS denotes supernatants
T11981 31733-31737 VBD denotes were
T11982 31738-31747 VBN denotes harvested
T11983 31748-31751 IN denotes for
T11984 31752-31757 NNP denotes ELISA
T11985 31758-31766 NN denotes analysis
T11986 31766-31767 . denotes .
T11987 31768-31771 DT denotes The
T11988 31772-31780 NN denotes presence
T11989 31781-31783 IN denotes of
T11990 31784-31791 NN denotes nitrite
T11991 31792-31794 IN denotes in
T11992 31795-31798 DT denotes the
T11993 31799-31811 NNS denotes supernatants
T11994 31812-31815 VBD denotes was
T11995 31816-31826 VBN denotes determined
T11996 31827-31832 VBG denotes using
T11997 31833-31836 DT denotes the
T11998 31837-31843 NNP denotes Griess
T11999 31844-31851 NN denotes reagent
T12000 31851-31852 . denotes .
T12090 31854-31872 NNP denotes Immunofluorescence
T12091 31873-31883 NNP denotes Fibroblast
T12092 31884-31887 CC denotes and
T12093 31888-31898 JJ denotes macrophage
T12094 31899-31904 NNS denotes cells
T12095 31905-31909 VBD denotes were
T12096 31910-31921 VBN denotes transfected
T12097 31922-31926 IN denotes with
T12098 31927-31940 NNP denotes pFLAG-SHARPIN
T12099 31940-31941 . denotes .
T12100 31942-31944 CD denotes 48
T12101 31945-31950 NNS denotes hours
T12102 31951-31956 RB denotes later
T12103 31956-31957 , denotes ,
T12104 31958-31963 NNS denotes cells
T12105 31964-31968 VBD denotes were
T12106 31969-31975 VBN denotes washed
T12107 31976-31983 RB denotes briefly
T12108 31984-31988 IN denotes with
T12109 31989-31992 NNP denotes PBS
T12110 31992-31993 , denotes ,
T12111 31994-31998 RB denotes then
T12112 31999-32004 VBN denotes fixed
T12113 32005-32007 IN denotes in
T12114 32008-32012 JJ denotes cold
T12115 32013-32021 NN denotes methanol
T12116 32022-32024 IN denotes at
T12117 32025-32026 CD denotes −
T12118 32026-32028 CD denotes 20
T12119 32028-32029 NN denotes °
T12120 32029-32030 NNP denotes C
T12121 32031-32034 IN denotes for
T12122 32035-32037 CD denotes 10
T12123 32038-32045 NNS denotes minutes
T12124 32046-32049 CC denotes and
T12125 32050-32054 JJ denotes cold
T12126 32055-32062 NN denotes acetone
T12127 32063-32065 IN denotes at
T12128 32066-32069 CD denotes -20
T12129 32069-32070 CD denotes °
T12130 32070-32071 NNP denotes C
T12131 32072-32075 IN denotes for
T12132 32076-32077 CD denotes 1
T12133 32078-32084 NN denotes minute
T12134 32084-32085 . denotes .
T12135 32086-32094 NNP denotes Incubate
T12136 32095-32100 NNS denotes cells
T12137 32101-32105 IN denotes with
T12138 32106-32109 NNP denotes PBS
T12139 32109-32111 CD denotes +1
T12140 32111-32112 NN denotes %
T12141 32113-32116 NNP denotes BSA
T12142 32117-32120 IN denotes for
T12143 32121-32123 CD denotes 15
T12144 32124-32131 NNS denotes minutes
T12145 32132-32134 TO denotes to
T12146 32135-32140 VB denotes block
T12147 32141-32153 JJ denotes non-specific
T12148 32154-32161 JJ denotes binding
T12149 32161-32162 . denotes .
T12150 32163-32168 VBN denotes Fixed
T12151 32169-32174 NNS denotes cells
T12152 32175-32179 VBD denotes were
T12153 32180-32184 RB denotes then
T12154 32185-32194 VBN denotes incubated
T12155 32195-32199 IN denotes with
T12156 32200-32209 JJ denotes anti-FLAG
T12157 32210-32211 -LRB- denotes (
T12158 32211-32212 CD denotes 1
T12159 32212-32213 CD denotes ∶
T12160 32213-32216 CD denotes 100
T12161 32216-32217 -RRB- denotes )
T12162 32218-32220 IN denotes as
T12163 32221-32224 DT denotes the
T12164 32225-32232 JJ denotes primary
T12165 32233-32241 NN denotes antibody
T12166 32242-32244 IN denotes at
T12167 32245-32249 NN denotes room
T12168 32250-32261 NN denotes temperature
T12169 32262-32265 IN denotes for
T12170 32266-32267 CD denotes 1
T12171 32268-32272 NN denotes hour
T12172 32272-32273 , denotes ,
T12173 32274-32282 VBN denotes followed
T12174 32283-32285 IN denotes by
T12175 32286-32291 CD denotes three
T12176 32292-32300 JJ denotes 5-minute
T12177 32301-32307 NNS denotes washes
T12178 32307-32308 . denotes .
T12179 32309-32314 NNS denotes Cells
T12180 32315-32319 VBD denotes were
T12181 32320-32327 JJ denotes further
T12182 32328-32337 VBN denotes incubated
T12183 32338-32342 IN denotes with
T12184 32343-32347 JJ denotes goat
T12185 32348-32359 JJ denotes anti-rabbit
T12186 32360-32368 NN denotes IgG-FITC
T12187 32369-32370 -LRB- denotes (
T12188 32370-32371 CD denotes 1
T12189 32371-32372 CD denotes ∶
T12190 32372-32375 CD denotes 100
T12191 32375-32376 -RRB- denotes )
T12192 32377-32379 IN denotes as
T12193 32380-32383 DT denotes the
T12194 32384-32393 JJ denotes secondary
T12195 32394-32402 NN denotes antibody
T12196 32403-32405 IN denotes at
T12197 32406-32410 NN denotes room
T12198 32411-32422 NN denotes temperature
T12199 32423-32426 IN denotes for
T12200 32427-32429 CD denotes 30
T12201 32430-32437 NNS denotes minutes
T12202 32437-32438 , denotes ,
T12203 32439-32447 VBN denotes followed
T12204 32448-32450 IN denotes by
T12205 32451-32456 CD denotes three
T12206 32457-32465 JJ denotes 5-minute
T12207 32466-32472 NNS denotes washes
T12208 32472-32473 . denotes .
T12209 32474-32479 NNS denotes Cells
T12210 32480-32484 VBD denotes were
T12211 32485-32489 RB denotes then
T12212 32490-32498 VBN denotes examined
T12213 32499-32504 VBG denotes using
T12214 32505-32512 JJ denotes inverse
T12215 32513-32525 NN denotes fluorescence
T12216 32526-32536 NN denotes microscope
T12217 32536-32537 . denotes .
T12386 32539-32550 NNP denotes Immunoblots
T12387 32551-32556 IN denotes After
T12388 32557-32563 VBG denotes adding
T12389 32564-32575 VBG denotes stimulating
T12390 32576-32583 NNS denotes ligands
T12391 32583-32584 , denotes ,
T12392 32585-32588 CD denotes 100
T12393 32589-32594 NNP denotes ng/mL
T12394 32595-32598 NNP denotes LPS
T12395 32599-32601 CC denotes or
T12396 32602-32604 CD denotes 25
T12397 32605-32607 NN denotes µg
T12398 32607-32608 CD denotes /
T12399 32608-32610 NNP denotes mL
T12400 32611-32615 NN denotes poly
T12401 32616-32617 PRP denotes I
T12402 32617-32618 : denotes :
T12403 32618-32619 NNP denotes C
T12404 32619-32620 , denotes ,
T12405 32621-32625 NNP denotes BMDC
T12406 32626-32630 VBD denotes were
T12407 32631-32640 VBN denotes collected
T12408 32641-32644 CC denotes and
T12409 32645-32650 VBN denotes lysed
T12410 32651-32653 IN denotes at
T12411 32654-32655 CD denotes 0
T12412 32655-32656 : denotes -
T12413 32656-32657 , denotes ,
T12414 32658-32660 CD denotes 15
T12415 32660-32661 : denotes -
T12416 32661-32662 , denotes ,
T12417 32663-32665 CD denotes 30
T12418 32665-32666 : denotes -
T12419 32666-32667 , denotes ,
T12420 32668-32671 CC denotes and
T12421 32672-32681 JJ denotes 60-minute
T12422 32682-32686 NN denotes time
T12423 32687-32693 NNS denotes points
T12424 32693-32694 . denotes .
T12425 32695-32706 NNS denotes Immunoblots
T12426 32707-32711 VBD denotes were
T12427 32712-32721 VBN denotes performed
T12428 32722-32726 IN denotes with
T12429 32727-32737 NNS denotes antibodies
T12430 32738-32739 -LRB- denotes (
T12431 32739-32743 NNP denotes Cell
T12432 32744-32753 NNP denotes Signaling
T12433 32754-32764 NNP denotes Technology
T12434 32764-32765 -RRB- denotes )
T12435 32766-32773 IN denotes against
T12436 32774-32780 NN denotes p-IKK1
T12437 32780-32781 NN denotes /
T12438 32781-32782 CD denotes 2
T12439 32782-32783 -LRB- denotes (
T12440 32783-32784 # denotes #
T12441 32784-32788 CD denotes 2697
T12442 32788-32789 -RRB- denotes )
T12443 32789-32790 , denotes ,
T12444 32791-32797 NNP denotes p-IκBα
T12445 32798-32799 -LRB- denotes (
T12446 32799-32800 # denotes #
T12447 32800-32804 CD denotes 9246
T12448 32804-32805 -RRB- denotes )
T12449 32805-32806 , denotes ,
T12450 32807-32811 NNP denotes IκBα
T12451 32812-32813 -LRB- denotes (
T12452 32813-32814 # denotes #
T12453 32814-32818 CD denotes 4814
T12454 32818-32819 -RRB- denotes )
T12455 32819-32820 , denotes ,
T12456 32821-32827 NN denotes p-TBK1
T12457 32827-32828 -LRB- denotes (
T12458 32828-32829 # denotes #
T12459 32829-32833 CD denotes 5483
T12460 32833-32834 -RRB- denotes )
T12461 32834-32835 , denotes ,
T12462 32836-32841 JJ denotes p-p38
T12463 32842-32843 -LRB- denotes (
T12464 32843-32844 # denotes #
T12465 32844-32848 CD denotes 9216
T12466 32848-32849 -RRB- denotes )
T12467 32849-32850 , denotes ,
T12468 32851-32854 CD denotes p38
T12469 32855-32856 -LRB- denotes (
T12470 32856-32857 # denotes #
T12471 32857-32861 CD denotes 9212
T12472 32861-32862 -RRB- denotes )
T12473 32862-32863 , denotes ,
T12474 32864-32870 JJ denotes p-ERK1
T12475 32870-32871 NN denotes /
T12476 32871-32872 CD denotes 2
T12477 32873-32874 -LRB- denotes (
T12478 32874-32875 # denotes #
T12479 32875-32879 CD denotes 4376
T12480 32879-32880 -RRB- denotes )
T12481 32880-32881 , denotes ,
T12482 32882-32885 CC denotes and
T12483 32886-32892 NNP denotes ERK1/2
T12484 32893-32894 -LRB- denotes (
T12485 32894-32895 # denotes #
T12486 32895-32899 CD denotes 4695
T12487 32899-32900 -RRB- denotes )
T12488 32900-32901 . denotes .
T12489 32902-32912 NN denotes Beta-actin
T12490 32913-32914 -LRB- denotes (
T12491 32914-32922 JJ denotes sc-47778
T12492 32922-32923 , denotes ,
T12493 32924-32929 NNP denotes Santa
T12494 32930-32934 NNP denotes Cruz
T12495 32935-32948 NNP denotes Biotechnology
T12496 32948-32949 -RRB- denotes )
T12497 32950-32953 VBD denotes was
T12498 32954-32958 VBN denotes used
T12499 32959-32961 IN denotes as
T12500 32962-32969 VBG denotes loading
T12501 32970-32977 NN denotes control
T12502 32977-32978 . denotes .
T12682 32980-32991 NNP denotes Statistical
T12683 32992-33000 NN denotes analysis
T12684 33001-33005 NNS denotes Data
T12685 33006-33009 VBP denotes are
T12686 33010-33019 VBN denotes expressed
T12687 33020-33022 IN denotes as
T12688 33023-33027 JJ denotes mean
T12689 33028-33029 NN denotes ±
T12690 33030-33032 NNP denotes SD
T12691 33032-33033 . denotes .
T12692 33034-33037 DT denotes The
T12693 33038-33049 JJ denotes statistical
T12694 33050-33062 NN denotes significance
T12695 33063-33065 IN denotes of
T12696 33066-33077 NNS denotes differences
T12697 33078-33080 IN denotes of
T12698 33081-33086 NNS denotes means
T12699 33087-33094 IN denotes between
T12700 33095-33107 JJ denotes experimental
T12701 33108-33114 NNS denotes groups
T12702 33115-33118 VBD denotes was
T12703 33119-33129 VBN denotes determined
T12704 33130-33132 IN denotes by
T12705 33133-33141 NNS denotes Students
T12706 33141-33142 POS denotes '
T12707 33143-33149 JJS denotes t-test
T12708 33149-33150 . denotes .
T12771 6096-6098 IN denotes In
T12772 6099-6103 NN denotes vivo
T12773 6104-6107 CC denotes and
T12774 6108-6110 IN denotes in
T12775 6111-6116 NN denotes vitro
T12776 6117-6125 NNS denotes features
T12777 6126-6128 IN denotes of
T12778 6129-6136 NNP denotes SHARPIN
T12779 6136-6137 . denotes .
T12780 6138-6139 -LRB- denotes (
T12781 6139-6140 NNP denotes A
T12782 6140-6141 -RRB- denotes )
T12783 6142-6147 NNP denotes COILS
T12784 6148-6151 CC denotes and
T12785 6152-6161 NNP denotes MotifScan
T12786 6162-6170 NNS denotes programs
T12787 6171-6175 VBD denotes were
T12788 6176-6180 VBN denotes used
T12789 6181-6183 TO denotes to
T12790 6184-6191 VB denotes predict
T12791 6192-6195 DT denotes the
T12792 6196-6204 NN denotes presence
T12793 6205-6207 IN denotes of
T12794 6208-6210 NNP denotes CC
T12795 6211-6212 -LRB- denotes (
T12796 6212-6223 NN denotes coiled-coil
T12797 6223-6224 -RRB- denotes )
T12798 6225-6231 NN denotes domain
T12799 6231-6232 , denotes ,
T12800 6233-6236 NNP denotes UBL
T12801 6237-6238 -LRB- denotes (
T12802 6238-6252 JJ denotes ubiquitin-like
T12803 6252-6253 -RRB- denotes )
T12804 6254-6260 NN denotes domain
T12805 6261-6264 CC denotes and
T12806 6265-6270 NNP denotes ZFRBP
T12807 6271-6272 -LRB- denotes (
T12808 6272-6283 NN denotes zinc-finger
T12809 6284-6295 NNP denotes Ran-Binding
T12810 6296-6303 NN denotes protein
T12811 6304-6305 CD denotes 2
T12812 6305-6306 -RRB- denotes )
T12813 6307-6313 NN denotes domain
T12814 6314-6319 WDT denotes which
T12815 6320-6324 VBP denotes form
T12816 6325-6332 JJ denotes similar
T12817 6333-6338 NN denotes motif
T12818 6339-6347 NNS denotes patterns
T12819 6348-6350 IN denotes in
T12820 6351-6354 DT denotes the
T12821 6355-6362 NNP denotes SHARPIN
T12822 6363-6370 NN denotes protein
T12823 6371-6373 IN denotes of
T12824 6374-6379 JJ denotes human
T12825 6379-6380 , denotes ,
T12826 6381-6386 NN denotes mouse
T12827 6387-6390 CC denotes and
T12828 6391-6394 NN denotes rat
T12829 6395-6402 NNS denotes origins
T12830 6402-6403 . denotes .
T12831 6404-6405 -LRB- denotes (
T12832 6405-6406 NNP denotes B
T12833 6406-6407 -RRB- denotes )
T12834 6408-6413 CD denotes Eight
T12835 6414-6422 JJ denotes week-old
T12836 6423-6430 NNS denotes females
T12837 6431-6433 IN denotes of
T12838 6434-6436 NNP denotes WT
T12839 6437-6440 CC denotes and
T12840 6441-6445 NN denotes cpdm
T12841 6446-6450 NNS denotes mice
T12842 6450-6451 . denotes .
T12843 6452-6455 DT denotes The
T12844 6456-6462 JJ denotes mutant
T12845 6463-6467 NNS denotes mice
T12846 6468-6469 -LRB- denotes (
T12847 6469-6474 IN denotes above
T12848 6474-6475 -RRB- denotes )
T12849 6476-6483 VB denotes develop
T12850 6484-6495 JJ denotes progressive
T12851 6496-6500 NN denotes skin
T12852 6501-6513 NN denotes inflammation
T12853 6514-6522 VBG denotes starting
T12854 6523-6525 IN denotes at
T12855 6526-6531 IN denotes about
T12856 6532-6536 CD denotes four
T12857 6537-6542 NNS denotes weeks
T12858 6542-6543 . denotes .
T12859 6544-6545 -LRB- denotes (
T12860 6545-6546 $ denotes C
T12861 6546-6547 -RRB- denotes )
T12862 6548-6562 JJ denotes Extramedullary
T12863 6563-6576 NN denotes hematopoiesis
T12864 6577-6583 VBZ denotes causes
T12865 6584-6590 JJ denotes marked
T12866 6591-6602 NN denotes enlargement
T12867 6603-6605 IN denotes of
T12868 6606-6609 DT denotes the
T12869 6610-6616 NN denotes spleen
T12870 6617-6619 IN denotes of
T12871 6620-6624 NN denotes cpdm
T12872 6625-6629 NNS denotes mice
T12873 6629-6630 . denotes .
T12874 6631-6632 -LRB- denotes (
T12875 6632-6633 NNP denotes D
T12876 6633-6634 -RRB- denotes )
T12877 6635-6639 NNP denotes BMDC
T12878 6640-6644 VBD denotes were
T12879 6645-6654 VBN denotes incubated
T12880 6655-6657 IN denotes in
T12881 6658-6661 DT denotes the
T12882 6662-6669 NN denotes absence
T12883 6670-6672 CC denotes or
T12884 6673-6681 NN denotes presence
T12885 6682-6684 IN denotes of
T12886 6685-6688 CD denotes 100
T12887 6689-6694 JJ denotes ng/ml
T12888 6695-6698 NNP denotes LPS
T12889 6699-6702 IN denotes for
T12890 6703-6704 CD denotes 4
T12891 6705-6710 NNS denotes hours
T12892 6710-6711 . denotes .
T12893 6712-6715 DT denotes The
T12894 6716-6720 NNP denotes mRNA
T12895 6721-6726 NN denotes level
T12896 6727-6729 IN denotes of
T12897 6730-6737 NNP denotes Sharpin
T12898 6738-6741 VBD denotes was
T12899 6742-6750 VBN denotes measured
T12900 6751-6753 IN denotes by
T12901 6754-6761 NNP denotes qRT-PCR
T12902 6762-6765 CC denotes and
T12903 6766-6775 VBN denotes presented
T12904 6776-6784 JJ denotes relative
T12905 6785-6787 TO denotes to
T12906 6788-6791 DT denotes the
T12907 6792-6796 NNP denotes mRNA
T12908 6797-6807 NN denotes expression
T12909 6808-6810 IN denotes in
T12910 6811-6825 JJ denotes non-stimulated
T12911 6826-6828 NNP denotes WT
T12912 6829-6833 NNP denotes BMDC
T12913 6833-6834 . denotes .
T12914 6835-6839 NNS denotes Bars
T12915 6840-6849 VBP denotes represent
T12916 6850-6853 DT denotes the
T12917 6854-6858 JJ denotes mean
T12918 6859-6860 NN denotes ±
T12919 6861-6865 NN denotes s.d.
T12920 6866-6868 IN denotes of
T12921 6869-6870 CD denotes 3
T12922 6871-6875 NNS denotes mice
T12923 6875-6876 . denotes .
T12924 6877-6878 SYM denotes *
T12925 6879-6880 NN denotes P
T12926 6880-6881 NN denotes <
T12927 6881-6885 CD denotes 0.05
T12928 6885-6886 : denotes ;
T12929 6887-6889 SYM denotes **
T12930 6890-6891 FW denotes P
T12931 6891-6892 FW denotes <
T12932 6892-6897 CD denotes 0.001
T12933 6897-6898 . denotes .
T12934 6899-6900 -LRB- denotes (
T12935 6900-6901 NNP denotes E
T12936 6901-6902 -RRB- denotes )
T12937 6903-6913 NNP denotes Fibroblast
T12938 6914-6915 -LRB- denotes (
T12939 6915-6921 NNP denotes NIH3T3
T12940 6921-6922 -RRB- denotes )
T12941 6923-6926 CC denotes and
T12942 6927-6938 NNS denotes macrophages
T12943 6939-6940 -LRB- denotes (
T12944 6940-6946 CD denotes RAW264
T12945 6946-6948 CD denotes .7
T12946 6948-6949 -RRB- denotes )
T12947 6950-6955 NNS denotes cells
T12948 6956-6960 VBD denotes were
T12949 6961-6972 VBN denotes transfected
T12950 6973-6977 IN denotes with
T12951 6978-6981 DT denotes the
T12952 6982-6992 NN denotes expression
T12953 6993-7000 VBD denotes plasmid
T12954 7001-7014 NNP denotes pFLAG-SHARPIN
T12955 7014-7015 . denotes .
T12956 7016-7021 IN denotes After
T12957 7022-7024 CD denotes 48
T12958 7025-7030 NNS denotes hours
T12959 7030-7031 , denotes ,
T12960 7032-7037 NNS denotes cells
T12961 7038-7042 VBD denotes were
T12962 7043-7048 VBN denotes fixed
T12963 7049-7052 CC denotes and
T12964 7053-7059 VBN denotes probed
T12965 7060-7064 IN denotes with
T12966 7065-7074 JJ denotes anti-FLAG
T12967 7075-7078 CC denotes and
T12968 7079-7094 JJ denotes FITC-conjugated
T12969 7095-7104 JJ denotes secondary
T12970 7105-7113 NN denotes antibody
T12971 7113-7114 . denotes .
T12972 7115-7121 NNP denotes Nuclei
T12973 7122-7126 VBD denotes were
T12974 7127-7134 VBN denotes stained
T12975 7135-7139 IN denotes with
T12976 7140-7144 NNP denotes DAPI
T12977 7144-7145 . denotes .
T12978 7146-7148 IN denotes In
T12979 7149-7153 DT denotes both
T12980 7154-7165 VBN denotes transfected
T12981 7166-7170 NN denotes cell
T12982 7171-7176 NNS denotes lines
T12983 7176-7177 , denotes ,
T12984 7178-7190 NNP denotes FLAG-SHARPIN
T12985 7191-7194 VBD denotes was
T12986 7195-7200 VBN denotes found
T12987 7201-7203 TO denotes to
T12988 7204-7206 VB denotes be
T12989 7207-7226 JJ denotes cytoplasm-localized
T12990 7226-7227 . denotes .
T13275 8298-8304 NN denotes Effect
T13276 8305-8307 IN denotes of
T13277 8308-8315 NNP denotes Sharpin
T13278 8316-8324 NN denotes mutation
T13279 8325-8327 IN denotes on
T13280 8328-8330 NNP denotes DC
T13281 8331-8345 NNS denotes subpopulations
T13282 8346-8349 CC denotes and
T13283 8350-8360 NN denotes maturation
T13284 8360-8361 . denotes .
T13285 8362-8363 -LRB- denotes (
T13286 8363-8364 NNP denotes A
T13287 8364-8365 -RRB- denotes )
T13288 8366-8373 NNP denotes Spleens
T13289 8374-8378 IN denotes from
T13290 8379-8381 NNP denotes WT
T13291 8382-8385 CC denotes and
T13292 8386-8390 NN denotes cpdm
T13293 8391-8395 NNS denotes mice
T13294 8396-8400 VBD denotes were
T13295 8401-8409 VBN denotes isolated
T13296 8410-8413 CC denotes and
T13297 8414-8421 JJ denotes subject
T13298 8422-8424 TO denotes to
T13299 8425-8436 VB denotes collagenase
T13300 8437-8440 CC denotes and
T13301 8441-8446 VB denotes DNase
T13302 8447-8456 NN denotes digestion
T13303 8456-8457 . denotes .
T13304 8458-8461 DT denotes The
T13305 8462-8470 VBN denotes obtained
T13306 8471-8478 JJ denotes splenic
T13307 8479-8490 NNS denotes homogenates
T13308 8491-8495 VBD denotes were
T13309 8496-8507 VBN denotes centrifuged
T13310 8508-8512 IN denotes over
T13311 8513-8514 DT denotes a
T13312 8515-8522 NNP denotes Percoll
T13313 8523-8531 NN denotes gradient
T13314 8532-8533 -LRB- denotes (
T13315 8533-8535 CD denotes 35
T13316 8535-8536 NN denotes %
T13317 8537-8540 CC denotes and
T13318 8541-8543 CD denotes 55
T13319 8543-8544 NN denotes %
T13320 8545-8552 NN denotes density
T13321 8552-8553 -RRB- denotes )
T13322 8554-8557 IN denotes for
T13323 8558-8560 CD denotes 15
T13324 8561-8568 NNS denotes minutes
T13325 8568-8569 . denotes .
T13326 8570-8573 DT denotes The
T13327 8574-8579 NNS denotes bands
T13328 8580-8582 IN denotes at
T13329 8583-8586 DT denotes the
T13330 8587-8589 CD denotes 35
T13331 8589-8590 NN denotes %
T13332 8590-8591 : denotes -
T13333 8591-8597 NN denotes medium
T13334 8598-8601 CC denotes and
T13335 8602-8605 DT denotes the
T13336 8606-8608 CD denotes 35
T13337 8609-8611 CD denotes 55
T13338 8611-8612 NN denotes %
T13339 8613-8622 NN denotes interface
T13340 8623-8627 VBD denotes were
T13341 8628-8634 VBN denotes pooled
T13342 8634-8635 , denotes ,
T13343 8636-8642 VBN denotes washed
T13344 8643-8646 CC denotes and
T13345 8647-8654 VBN denotes stained
T13346 8655-8659 IN denotes with
T13347 8660-8661 DT denotes a
T13348 8662-8673 NN denotes combination
T13349 8674-8676 IN denotes of
T13350 8677-8684 JJ denotes various
T13351 8685-8695 NNS denotes antibodies
T13352 8696-8698 TO denotes to
T13353 8699-8704 VB denotes stain
T13354 8705-8714 JJ denotes different
T13355 8715-8717 NN denotes DC
T13356 8718-8725 NNS denotes subsets
T13357 8725-8726 , denotes ,
T13358 8727-8739 JJ denotes conventional
T13359 8740-8745 JJ denotes CD11c
T13360 8745-8746 NN denotes +
T13361 8746-8750 NNP denotes CD8α
T13362 8750-8751 NNP denotes +
T13363 8751-8752 , denotes ,
T13364 8753-8758 NNP denotes CD11c
T13365 8758-8759 NNP denotes +
T13366 8759-8763 NNP denotes CD8α
T13367 8763-8764 NNP denotes −
T13368 8765-8768 CC denotes and
T13369 8769-8781 JJ denotes plasmacytoid
T13370 8782-8784 NNP denotes DC
T13371 8785-8786 -LRB- denotes (
T13372 8786-8791 NNP denotes CD11c
T13373 8791-8792 NNP denotes −
T13374 8792-8798 NNP denotes PDCA-1
T13375 8798-8799 NNP denotes +
T13376 8799-8800 -RRB- denotes )
T13377 8800-8801 . denotes .
T13378 8801-8804 DT denotes The
T13379 8805-8808 JJ denotes top
T13380 8809-8815 NNS denotes panels
T13381 8816-8820 VBD denotes were
T13382 8821-8826 VBN denotes gated
T13383 8827-8829 IN denotes on
T13384 8830-8840 NNP denotes FSChiSSClo
T13385 8841-8846 NNS denotes cells
T13386 8847-8849 TO denotes to
T13387 8850-8854 VB denotes show
T13388 8855-8863 JJ denotes separate
T13389 8864-8875 NNS denotes populations
T13390 8876-8878 IN denotes of
T13391 8879-8884 JJ denotes CD11c
T13392 8884-8885 NN denotes +
T13393 8885-8891 NN denotes PDCA-1
T13394 8891-8892 NN denotes −
T13395 8893-8896 CC denotes and
T13396 8897-8902 JJ denotes CD11c
T13397 8902-8903 NN denotes −
T13398 8903-8909 NN denotes PDCA-1
T13399 8909-8910 NN denotes +
T13400 8911-8916 NNS denotes cells
T13401 8916-8917 . denotes .
T13402 8918-8925 JJ denotes Further
T13403 8926-8932 NN denotes gating
T13404 8933-8935 IN denotes on
T13405 8936-8939 DT denotes the
T13406 8940-8945 JJ denotes CD11c
T13407 8945-8946 NN denotes +
T13408 8946-8952 NN denotes PDCA-1
T13409 8952-8953 NN denotes −
T13410 8954-8967 NN denotes subpopulation
T13411 8968-8972 VBD denotes gave
T13412 8973-8976 DT denotes the
T13413 8977-8983 JJ denotes bottom
T13414 8984-8989 NN denotes panel
T13415 8990-8994 WDT denotes that
T13416 8995-9001 VBD denotes showed
T13417 9002-9005 CD denotes two
T13418 9006-9014 JJ denotes distinct
T13419 9015-9020 NNS denotes pools
T13420 9021-9023 IN denotes of
T13421 9024-9029 JJ denotes CD11c
T13422 9029-9030 NN denotes +
T13423 9030-9034 NNP denotes CD8α
T13424 9034-9035 NNP denotes +
T13425 9036-9039 CC denotes and
T13426 9040-9045 NNP denotes CD11c
T13427 9045-9046 VBP denotes +
T13428 9046-9050 JJ denotes CD8α
T13429 9050-9051 NN denotes −
T13430 9052-9057 NNS denotes cells
T13431 9057-9058 . denotes .
T13432 9059-9070 NNS denotes Percentages
T13433 9071-9075 VBD denotes were
T13434 9076-9086 VBN denotes calculated
T13435 9087-9092 VBN denotes based
T13436 9093-9095 IN denotes on
T13437 9096-9099 DT denotes the
T13438 9100-9108 JJ denotes parental
T13439 9109-9119 NN denotes population
T13440 9120-9123 CC denotes and
T13441 9124-9128 VBD denotes were
T13442 9129-9141 RB denotes additionally
T13443 9142-9147 VBN denotes shown
T13444 9148-9150 IN denotes as
T13445 9151-9154 NN denotes bar
T13446 9155-9161 NNS denotes graphs
T13447 9162-9163 -LRB- denotes (
T13448 9163-9164 FW denotes n
T13449 9165-9166 SYM denotes =
T13450 9167-9168 LS denotes 2
T13451 9168-9169 -RRB- denotes )
T13452 9170-9171 -LRB- denotes (
T13453 9171-9172 NNP denotes B
T13454 9172-9173 -RRB- denotes )
T13455 9173-9174 . denotes .
T13456 9175-9176 -LRB- denotes (
T13457 9176-9177 NNP denotes C
T13458 9177-9178 -RRB- denotes )
T13459 9179-9181 NNP denotes WT
T13460 9182-9185 CC denotes and
T13461 9186-9190 VB denotes cpdm
T13462 9191-9195 NNP denotes BMDC
T13463 9196-9197 -LRB- denotes (
T13464 9197-9198 CD denotes 5
T13465 9198-9199 CD denotes ×
T13466 9199-9202 CD denotes 105
T13467 9202-9203 -RRB- denotes )
T13468 9204-9209 NNS denotes cells
T13469 9210-9214 VBD denotes were
T13470 9215-9225 VBN denotes stimulated
T13471 9226-9230 IN denotes with
T13472 9231-9237 NN denotes medium
T13473 9237-9238 , denotes ,
T13474 9239-9242 CD denotes 100
T13475 9243-9248 JJ denotes ng/ml
T13476 9249-9252 NNP denotes LPS
T13477 9253-9255 CC denotes or
T13478 9256-9258 CD denotes 25
T13479 9259-9261 NN denotes µg
T13480 9261-9262 NN denotes /
T13481 9262-9264 NN denotes ml
T13482 9265-9269 NN denotes poly
T13483 9270-9271 PRP denotes I
T13484 9271-9272 : denotes :
T13485 9272-9273 NNP denotes C
T13486 9274-9277 IN denotes for
T13487 9278-9280 CD denotes 24
T13488 9281-9286 NNS denotes hours
T13489 9286-9287 . denotes .
T13490 9288-9291 DT denotes The
T13491 9292-9297 NNS denotes cells
T13492 9298-9302 VBD denotes were
T13493 9303-9310 VBN denotes labeled
T13494 9311-9315 IN denotes with
T13495 9316-9326 JJ denotes PE-labeled
T13496 9327-9336 JJ denotes anti-CD40
T13497 9336-9337 , denotes ,
T13498 9338-9347 JJ denotes anti-CD80
T13499 9347-9348 , denotes ,
T13500 9349-9352 CC denotes and
T13501 9353-9362 NNP denotes anti-CD86
T13502 9362-9363 , denotes ,
T13503 9364-9367 CC denotes and
T13504 9368-9377 VBN denotes subjected
T13505 9378-9380 TO denotes to
T13506 9381-9385 VB denotes flow
T13507 9386-9395 JJ denotes cytometry
T13508 9396-9404 NN denotes analysis
T13509 9404-9405 . denotes .
T13510 9406-9409 DT denotes The
T13511 9410-9421 NNS denotes populations
T13512 9422-9427 VBN denotes shown
T13513 9428-9430 IN denotes in
T13514 9431-9441 NNS denotes histograms
T13515 9442-9446 VBD denotes were
T13516 9447-9452 VBN denotes gated
T13517 9453-9455 IN denotes on
T13518 9456-9461 JJ denotes CD11c
T13519 9461-9462 NN denotes +
T13520 9463-9468 NNS denotes cells
T13521 9468-9469 . denotes .
T13522 9470-9479 JJ denotes Unstained
T13523 9480-9485 NNS denotes cells
T13524 9486-9492 VBD denotes served
T13525 9493-9495 IN denotes as
T13526 9496-9504 JJ denotes negative
T13527 9505-9513 NNS denotes controls
T13528 9513-9514 . denotes .
T13529 9515-9522 NNS denotes Results
T13530 9523-9526 VBP denotes are
T13531 9527-9541 NN denotes representative
T13532 9542-9544 IN denotes of
T13533 9545-9548 CD denotes two
T13534 9549-9560 JJ denotes independent
T13535 9561-9572 NNS denotes experiments
T13536 9572-9573 . denotes .
T13813 11390-11399 JJ denotes Defective
T13814 11400-11410 NN denotes production
T13815 11411-11413 IN denotes of
T13816 11414-11430 JJ denotes pro-inflammatory
T13817 11431-11440 NNS denotes mediators
T13818 11441-11445 IN denotes from
T13819 11446-11456 VBN denotes stimulated
T13820 11457-11461 NN denotes cpdm
T13821 11462-11466 NNP denotes BMDC
T13822 11466-11467 . denotes .
T13823 11468-11469 -LRB- denotes (
T13824 11469-11470 DT denotes A
T13825 11471-11472 NNP denotes D
T13826 11472-11473 -RRB- denotes )
T13827 11474-11482 NNP denotes Cultured
T13828 11483-11485 NNP denotes WT
T13829 11486-11489 CC denotes and
T13830 11490-11494 VB denotes cpdm
T13831 11495-11499 NNP denotes BMDC
T13832 11500-11501 -LRB- denotes (
T13833 11501-11502 CD denotes 1
T13834 11502-11503 CD denotes ×
T13835 11503-11506 CD denotes 105
T13836 11507-11512 NNS denotes cells
T13837 11513-11515 IN denotes in
T13838 11516-11519 CD denotes 0.1
T13839 11520-11522 NN denotes ml
T13840 11523-11531 JJ denotes complete
T13841 11532-11538 NN denotes medium
T13842 11538-11539 -RRB- denotes )
T13843 11540-11544 VBD denotes were
T13844 11545-11551 VBN denotes washed
T13845 11552-11555 CC denotes and
T13846 11556-11566 VBN denotes stimulated
T13847 11567-11571 IN denotes with
T13848 11572-11578 NN denotes medium
T13849 11578-11579 , denotes ,
T13850 11580-11583 CD denotes 100
T13851 11584-11589 JJ denotes ng/ml
T13852 11590-11593 NNP denotes LPS
T13853 11594-11596 CC denotes or
T13854 11597-11599 CD denotes 25
T13855 11600-11602 NN denotes µg
T13856 11602-11603 NN denotes /
T13857 11603-11605 NN denotes ml
T13858 11606-11610 NN denotes poly
T13859 11611-11612 PRP denotes I
T13860 11612-11613 : denotes :
T13861 11613-11614 NNP denotes C
T13862 11615-11618 IN denotes for
T13863 11619-11621 CD denotes 24
T13864 11622-11627 NNS denotes hours
T13865 11627-11628 . denotes .
T13866 11629-11641 NNS denotes Supernatants
T13867 11642-11646 VBD denotes were
T13868 11647-11656 VBN denotes collected
T13869 11657-11660 IN denotes for
T13870 11661-11666 NNP denotes ELISA
T13871 11667-11669 IN denotes of
T13872 11670-11677 NNP denotes IL12P70
T13873 11677-11678 , denotes ,
T13874 11679-11682 NNP denotes IL6
T13875 11682-11683 , denotes ,
T13876 11684-11687 CC denotes and
T13877 11688-11693 NNP denotes GMCSF
T13878 11693-11694 , denotes ,
T13879 11695-11698 CC denotes and
T13880 11699-11702 IN denotes for
T13881 11703-11717 NN denotes quantification
T13882 11718-11720 IN denotes of
T13883 11721-11727 JJ denotes nitric
T13884 11728-11733 NN denotes oxide
T13885 11734-11735 -LRB- denotes (
T13886 11735-11737 UH denotes NO
T13887 11737-11738 -RRB- denotes )
T13888 11738-11739 . denotes .
T13889 11740-11741 -LRB- denotes (
T13890 11741-11742 NNP denotes E
T13891 11743-11744 NN denotes F
T13892 11744-11745 -RRB- denotes )
T13893 11746-11754 NNP denotes Gradient
T13894 11755-11762 NNS denotes numbers
T13895 11763-11764 -LRB- denotes (
T13896 11764-11765 CD denotes 1
T13897 11765-11766 CD denotes ×
T13898 11766-11769 CD denotes 104
T13899 11769-11770 , denotes ,
T13900 11771-11772 CD denotes 2
T13901 11772-11773 CD denotes ×
T13902 11773-11776 CD denotes 104
T13903 11776-11777 , denotes ,
T13904 11778-11781 CC denotes and
T13905 11782-11783 CD denotes 4
T13906 11783-11784 NN denotes ×
T13907 11784-11787 CD denotes 104
T13908 11788-11793 NNS denotes cells
T13909 11794-11796 IN denotes in
T13910 11797-11800 CD denotes 0.1
T13911 11801-11803 NNP denotes mL
T13912 11804-11812 JJ denotes complete
T13913 11813-11819 NN denotes medium
T13914 11819-11820 -RRB- denotes )
T13915 11821-11823 IN denotes of
T13916 11824-11828 NNP denotes BMDC
T13917 11829-11833 VBD denotes were
T13918 11834-11838 VBN denotes used
T13919 11839-11845 CD denotes for100
T13920 11846-11851 NNP denotes ng/mL
T13921 11852-11855 NNP denotes LPS
T13922 11856-11867 NN denotes stimulation
T13923 11867-11868 . denotes .
T13924 11869-11874 IN denotes After
T13925 11875-11877 CD denotes 24
T13926 11878-11883 NNS denotes hours
T13927 11883-11884 , denotes ,
T13928 11885-11888 DT denotes the
T13929 11889-11896 NNS denotes amounts
T13930 11897-11899 IN denotes of
T13931 11900-11907 NNP denotes IL12P70
T13932 11908-11911 CC denotes and
T13933 11912-11915 NNP denotes IL6
T13934 11916-11920 IN denotes from
T13935 11921-11924 DT denotes the
T13936 11925-11936 JJ denotes supernatant
T13937 11937-11941 VBD denotes were
T13938 11942-11950 VBN denotes measured
T13939 11950-11951 . denotes .
T13940 11952-11953 -LRB- denotes (
T13941 11953-11954 NNP denotes G
T13942 11954-11955 -RRB- denotes )
T13943 11956-11959 DT denotes The
T13944 11960-11964 NN denotes cpdm
T13945 11965-11969 NNS denotes mice
T13946 11970-11977 VBN denotes rescued
T13947 11978-11980 IN denotes by
T13948 11981-11999 VBG denotes Sharpin-containing
T13949 12000-12003 NNP denotes BAC
T13950 12004-12007 VBD denotes had
T13951 12008-12016 JJ denotes complete
T13952 12017-12026 NN denotes remission
T13953 12027-12029 IN denotes of
T13954 12030-12033 DT denotes the
T13955 12034-12046 JJ denotes inflammatory
T13956 12047-12056 NN denotes phenotype
T13957 12057-12058 NN denotes [
T13958 12058-12059 CD denotes 2
T13959 12059-12060 NNP denotes ]
T13960 12060-12061 . denotes .
T13961 12062-12066 NNP denotes BMDC
T13962 12067-12076 VBD denotes developed
T13963 12077-12081 IN denotes from
T13964 12082-12086 NN denotes cpdm
T13965 12087-12090 CC denotes and
T13966 12091-12098 VBD denotes rescued
T13967 12099-12103 NN denotes cpdm
T13968 12104-12108 NNS denotes mice
T13969 12109-12113 VBD denotes were
T13970 12114-12120 VBN denotes plated
T13971 12121-12122 -LRB- denotes (
T13972 12122-12123 CD denotes 1
T13973 12123-12124 CD denotes ×
T13974 12124-12127 CD denotes 106
T13975 12128-12133 NNS denotes cells
T13976 12134-12136 IN denotes in
T13977 12137-12140 CD denotes 0.3
T13978 12141-12143 NNP denotes mL
T13979 12143-12144 -RRB- denotes )
T13980 12145-12148 CC denotes and
T13981 12149-12159 VBN denotes stimulated
T13982 12160-12164 IN denotes with
T13983 12165-12168 CD denotes 100
T13984 12169-12174 NNP denotes ng/mL
T13985 12175-12178 NNP denotes LPS
T13986 12178-12179 . denotes .
T13987 12180-12185 IN denotes After
T13988 12186-12188 CD denotes 24
T13989 12189-12194 NNS denotes hours
T13990 12194-12195 , denotes ,
T13991 12196-12208 NNS denotes supernatants
T13992 12209-12213 VBD denotes were
T13993 12214-12223 VBN denotes collected
T13994 12224-12227 IN denotes for
T13995 12228-12236 NN denotes analysis
T13996 12237-12239 IN denotes of
T13997 12240-12247 CD denotes IL12P70
T13998 12248-12258 NN denotes production
T13999 12258-12259 . denotes .
T14000 12260-12264 NNS denotes Data
T14001 12265-12268 VBP denotes are
T14002 12269-12283 NN denotes representative
T14003 12284-12286 IN denotes of
T14004 12287-12292 CD denotes three
T14005 12293-12304 JJ denotes independent
T14006 12305-12316 NNS denotes experiments
T14007 12316-12317 . denotes .
T14008 12318-12319 SYM denotes *
T14009 12320-12321 NN denotes P
T14010 12321-12322 NN denotes <
T14011 12322-12326 CD denotes 0.01
T14012 12326-12327 : denotes ;
T14013 12328-12330 SYM denotes **
T14014 12331-12332 FW denotes P
T14015 12332-12333 FW denotes <
T14016 12333-12338 CD denotes 0.005
T14017 12338-12339 . denotes .
T14261 12384-12393 JJ denotes Decreased
T14262 12394-12398 NN denotes mRNA
T14263 12399-12405 NNS denotes levels
T14264 12406-12408 IN denotes of
T14265 12409-12421 JJ denotes inflammatory
T14266 12422-12431 NNS denotes cytokines
T14267 12432-12436 IN denotes from
T14268 12437-12441 NN denotes cpdm
T14269 12442-12446 NNP denotes BMDC
T14270 12446-12447 . denotes .
T14271 12448-12456 NNP denotes Cultured
T14272 12457-12459 NNP denotes WT
T14273 12460-12463 CC denotes and
T14274 12464-12468 VB denotes cpdm
T14275 12469-12473 NNP denotes BMDC
T14276 12474-12475 -LRB- denotes (
T14277 12475-12476 CD denotes 5
T14278 12476-12477 CD denotes ×
T14279 12477-12480 CD denotes 105
T14280 12481-12486 NNS denotes cells
T14281 12487-12489 IN denotes in
T14282 12490-12493 CD denotes 0.2
T14283 12494-12496 NN denotes ml
T14284 12497-12505 JJ denotes complete
T14285 12506-12512 NN denotes medium
T14286 12512-12513 -RRB- denotes )
T14287 12514-12518 VBD denotes were
T14288 12519-12525 VBN denotes washed
T14289 12526-12529 CC denotes and
T14290 12530-12540 VBN denotes stimulated
T14291 12541-12545 IN denotes with
T14292 12546-12549 CD denotes 100
T14293 12550-12555 JJ denotes ng/ml
T14294 12556-12559 NNP denotes LPS
T14295 12560-12561 -LRB- denotes (
T14296 12561-12562 NNP denotes A
T14297 12562-12563 , denotes ,
T14298 12563-12564 NNP denotes C
T14299 12564-12565 , denotes ,
T14300 12565-12566 NNP denotes E
T14301 12566-12567 , denotes ,
T14302 12567-12568 NNP denotes G
T14303 12568-12569 -RRB- denotes )
T14304 12570-12572 CC denotes or
T14305 12573-12575 CD denotes 25
T14306 12576-12578 NN denotes µg
T14307 12578-12579 NN denotes /
T14308 12579-12581 NN denotes ml
T14309 12582-12586 NN denotes poly
T14310 12587-12588 PRP denotes I
T14311 12588-12589 : denotes :
T14312 12589-12590 NNP denotes C
T14313 12591-12592 -LRB- denotes (
T14314 12592-12593 NNP denotes B
T14315 12593-12594 , denotes ,
T14316 12594-12595 NNP denotes D
T14317 12595-12596 , denotes ,
T14318 12596-12597 NN denotes F
T14319 12597-12598 , denotes ,
T14320 12598-12599 NNP denotes H
T14321 12599-12600 -RRB- denotes )
T14322 12600-12601 . denotes .
T14323 12602-12604 IN denotes At
T14324 12605-12606 CD denotes 0
T14325 12606-12607 , denotes ,
T14326 12608-12609 CD denotes 1
T14327 12610-12613 CC denotes and
T14328 12614-12615 CD denotes 2
T14329 12616-12621 NNS denotes hours
T14330 12621-12622 , denotes ,
T14331 12623-12628 JJ denotes total
T14332 12629-12632 NNP denotes RNA
T14333 12633-12636 VBD denotes was
T14334 12637-12646 VBN denotes extracted
T14335 12647-12650 CC denotes and
T14336 12651-12658 JJ denotes subject
T14337 12659-12661 TO denotes to
T14338 12662-12669 NNP denotes qRT-PCR
T14339 12670-12672 TO denotes to
T14340 12673-12680 VB denotes measure
T14341 12681-12684 DT denotes the
T14342 12685-12695 NN denotes expression
T14343 12696-12698 IN denotes of
T14344 12699-12706 NNP denotes Il12p40
T14345 12707-12708 -LRB- denotes (
T14346 12708-12709 NNP denotes A
T14347 12709-12710 , denotes ,
T14348 12710-12711 NNP denotes B
T14349 12711-12712 -RRB- denotes )
T14350 12712-12713 , denotes ,
T14351 12714-12717 NNP denotes Il6
T14352 12718-12719 -LRB- denotes (
T14353 12719-12720 NNP denotes C
T14354 12720-12721 , denotes ,
T14355 12721-12722 NNP denotes D
T14356 12722-12723 -RRB- denotes )
T14357 12723-12724 , denotes ,
T14358 12725-12730 NNP denotes Gmcsf
T14359 12731-12732 -LRB- denotes (
T14360 12732-12733 NNP denotes E
T14361 12733-12734 , denotes ,
T14362 12734-12735 NN denotes F
T14363 12735-12736 -RRB- denotes )
T14364 12736-12737 , denotes ,
T14365 12738-12741 CC denotes and
T14366 12742-12746 NNP denotes Ifnb
T14367 12747-12748 -LRB- denotes (
T14368 12748-12749 NNP denotes G
T14369 12749-12750 , denotes ,
T14370 12750-12751 NNP denotes H
T14371 12751-12752 -RRB- denotes )
T14372 12753-12757 NNP denotes mRNA
T14373 12757-12758 . denotes .
T14374 12759-12763 NNS denotes Bars
T14375 12764-12773 VBP denotes represent
T14376 12774-12778 JJ denotes mean
T14377 12779-12780 NN denotes ±
T14378 12781-12783 NNP denotes SD
T14379 12783-12784 . denotes .
T14380 12785-12789 NNS denotes Data
T14381 12790-12793 VBP denotes are
T14382 12794-12808 NN denotes representative
T14383 12809-12811 IN denotes of
T14384 12812-12815 CD denotes two
T14385 12816-12827 JJ denotes independent
T14386 12828-12839 NNS denotes experiments
T14387 12839-12840 . denotes .
T14584 14284-14290 JJ denotes Normal
T14585 14291-14295 CD denotes TLR4
T14586 14296-14299 CC denotes and
T14587 14300-14303 CD denotes MD2
T14588 14304-14314 NN denotes expression
T14589 14315-14318 CC denotes and
T14590 14319-14328 VBD denotes decreased
T14591 14329-14333 NNP denotes IL10
T14592 14334-14343 NN denotes secretion
T14593 14344-14347 CC denotes and
T14594 14348-14351 NNP denotes A20
T14595 14352-14362 NN denotes expression
T14596 14363-14365 IN denotes by
T14597 14366-14370 NN denotes cpdm
T14598 14371-14375 NNP denotes BMDC
T14599 14375-14376 . denotes .
T14600 14377-14378 -LRB- denotes (
T14601 14378-14379 NNP denotes A
T14602 14379-14380 -RRB- denotes )
T14603 14381-14393 NNP denotes Unstimulated
T14604 14394-14396 NNP denotes WT
T14605 14397-14400 CC denotes and
T14606 14401-14405 VB denotes cpdm
T14607 14406-14410 NNP denotes BMDC
T14608 14411-14412 -LRB- denotes (
T14609 14412-14413 CD denotes 5
T14610 14413-14414 CD denotes ×
T14611 14414-14417 CD denotes 105
T14612 14417-14418 -RRB- denotes )
T14613 14419-14423 VBD denotes were
T14614 14424-14431 VBN denotes labeled
T14615 14432-14436 IN denotes with
T14616 14437-14447 JJ denotes PE-labeled
T14617 14448-14457 JJ denotes anti-TLR4
T14618 14457-14458 NN denotes /
T14619 14458-14461 CD denotes MD2
T14620 14462-14464 CC denotes or
T14621 14465-14474 JJ denotes anti-CD14
T14622 14475-14478 CC denotes and
T14623 14479-14483 RB denotes then
T14624 14484-14491 JJ denotes subject
T14625 14492-14494 TO denotes to
T14626 14495-14499 VB denotes flow
T14627 14500-14509 JJ denotes cytometry
T14628 14510-14518 NN denotes analysis
T14629 14518-14519 . denotes .
T14630 14520-14529 JJ denotes Unstained
T14631 14530-14535 NNS denotes cells
T14632 14536-14541 VBP denotes serve
T14633 14542-14544 IN denotes as
T14634 14545-14553 JJ denotes negative
T14635 14554-14562 NNS denotes controls
T14636 14562-14563 . denotes .
T14637 14564-14565 -LRB- denotes (
T14638 14565-14566 NNP denotes B
T14639 14772-14773 -RRB- denotes )
T14640 14774-14782 NNP denotes Cultured
T14641 14783-14785 NNP denotes WT
T14642 14786-14789 CC denotes and
T14643 14790-14794 VB denotes cpdm
T14644 14795-14799 NNP denotes BMDC
T14645 14800-14801 -LRB- denotes (
T14646 14801-14802 CD denotes 5
T14647 14802-14803 CD denotes ×
T14648 14566-14567 -RRB- denotes )
T14649 14568-14576 NNP denotes Cultured
T14650 14577-14579 NNP denotes WT
T14651 14580-14583 CC denotes and
T14652 14584-14588 VB denotes cpdm
T14653 14589-14593 NNP denotes BMDC
T14654 14594-14595 -LRB- denotes (
T14655 14595-14596 CD denotes 1
T14656 14596-14597 CD denotes ×
T14657 14597-14600 CD denotes 105
T14658 14601-14606 NNS denotes cells
T14659 14607-14609 IN denotes in
T14660 14610-14613 CD denotes 0.1
T14661 14614-14616 NN denotes ml
T14662 14617-14625 JJ denotes complete
T14663 14626-14632 NN denotes medium
T14664 14632-14633 -RRB- denotes )
T14665 14634-14638 VBD denotes were
T14666 14639-14645 VBN denotes washed
T14667 14646-14649 CC denotes and
T14668 14650-14660 VBN denotes stimulated
T14669 14661-14665 IN denotes with
T14670 14666-14672 NN denotes medium
T14671 14672-14673 , denotes ,
T14672 14674-14677 CD denotes 100
T14673 14678-14683 JJ denotes ng/ml
T14674 14684-14687 NNP denotes LPS
T14675 14688-14690 CC denotes or
T14676 14691-14693 CD denotes 25
T14677 14694-14696 NN denotes µg
T14678 14696-14697 NN denotes /
T14679 14697-14699 NN denotes ml
T14680 14700-14704 NN denotes poly
T14681 14705-14706 PRP denotes I
T14682 14706-14707 : denotes :
T14683 14707-14708 NNP denotes C
T14684 14709-14712 IN denotes for
T14685 14713-14715 CD denotes 24
T14686 14716-14721 NNS denotes hours
T14687 14721-14722 . denotes .
T14688 14723-14735 NNS denotes Supernatants
T14689 14736-14740 VBD denotes were
T14690 14741-14750 VBN denotes collected
T14691 14751-14754 IN denotes for
T14692 14755-14760 NNP denotes ELISA
T14693 14761-14763 IN denotes of
T14694 14764-14768 NNP denotes IL10
T14695 14768-14769 . denotes .
T14696 14770-14771 -LRB- denotes (
T14697 14771-14772 NNP denotes C
T14860 14852-14855 CC denotes and
T14861 14856-14866 VBN denotes stimulated
T14862 14867-14871 IN denotes with
T14863 14872-14875 CD denotes 100
T14864 14876-14881 JJ denotes ng/ml
T14865 14882-14885 NNP denotes LPS
T14866 14886-14888 CC denotes or
T14867 14889-14891 CD denotes 25
T14868 14892-14894 NN denotes µg
T14869 14894-14895 NN denotes /
T14870 14895-14897 NN denotes ml
T14871 14898-14902 NN denotes poly
T14872 14903-14904 PRP denotes I
T14873 14904-14905 : denotes :
T14874 14905-14906 NNP denotes C
T14875 14906-14907 . denotes .
T14876 14908-14910 IN denotes At
T14877 14911-14912 CD denotes 0
T14878 14912-14913 , denotes ,
T14879 14914-14915 CD denotes 1
T14880 14915-14916 , denotes ,
T14881 14917-14920 CC denotes and
T14882 14921-14922 CD denotes 2
T14883 14923-14928 NNS denotes hours
T14884 14928-14929 , denotes ,
T14885 14930-14935 JJ denotes total
T14886 14936-14939 NNP denotes RNA
T14887 14940-14943 VBD denotes was
T14888 14944-14953 VBN denotes extracted
T14889 14954-14957 CC denotes and
T14890 14958-14965 JJ denotes subject
T14891 14966-14968 TO denotes to
T14892 14969-14976 NNP denotes qRT-PCR
T14893 14977-14979 TO denotes to
T14894 14980-14987 VB denotes measure
T14895 14988-14991 DT denotes the
T14896 14992-15002 NN denotes production
T14897 15003-15005 IN denotes of
T14898 15006-15009 NNP denotes A20
T14899 15009-15010 . denotes .
T15127 16085-16095 NNP denotes Inhibition
T15128 16096-16098 IN denotes of
T15129 16099-16104 NNP denotes NF-κB
T15130 16105-16114 VBG denotes signaling
T15131 16115-16117 IN denotes in
T15132 16118-16122 NN denotes cpdm
T15133 16123-16127 NNP denotes BMDC
T15134 16127-16128 . denotes .
T15135 16129-16131 NNP denotes WT
T15136 16132-16135 CC denotes and
T15137 16136-16140 VB denotes cpdm
T15138 16141-16145 NNP denotes BMDC
T15139 16146-16147 -LRB- denotes (
T15140 16147-16148 CD denotes 2
T15141 16148-16149 CD denotes ×
T15142 16149-16152 CD denotes 106
T15143 16153-16158 NNS denotes cells
T15144 16159-16161 IN denotes in
T15145 16162-16165 CD denotes 0.5
T15146 16166-16168 NNP denotes mL
T15147 16169-16177 JJ denotes complete
T15148 16178-16184 NN denotes medium
T15149 16184-16185 -RRB- denotes )
T15150 16186-16190 VBD denotes were
T15151 16191-16201 VBN denotes stimulated
T15152 16202-16206 IN denotes with
T15153 16207-16210 CD denotes 100
T15154 16211-16216 NNP denotes ng/mL
T15155 16217-16220 NNP denotes LPS
T15156 16221-16222 -LRB- denotes (
T15157 16222-16223 NNP denotes A
T15158 16223-16224 -RRB- denotes )
T15159 16225-16227 CC denotes or
T15160 16228-16230 CD denotes 25
T15161 16231-16233 NN denotes µg
T15162 16233-16234 CD denotes /
T15163 16234-16236 NNP denotes mL
T15164 16237-16241 NN denotes poly
T15165 16242-16243 PRP denotes I
T15166 16243-16244 : denotes :
T15167 16244-16245 NNP denotes C
T15168 16246-16247 -LRB- denotes (
T15169 16247-16248 NNP denotes B
T15170 16248-16249 -RRB- denotes )
T15171 16249-16250 . denotes .
T15172 16251-16253 IN denotes At
T15173 16254-16255 CD denotes 0
T15174 16255-16256 , denotes ,
T15175 16257-16259 CD denotes 15
T15176 16259-16260 , denotes ,
T15177 16261-16263 CD denotes 30
T15178 16263-16264 , denotes ,
T15179 16265-16268 CC denotes and
T15180 16269-16271 CD denotes 60
T15181 16272-16279 NNS denotes minutes
T15182 16279-16280 , denotes ,
T15183 16281-16291 JJ denotes whole-cell
T15184 16292-16299 NNS denotes lysates
T15185 16300-16304 VBD denotes were
T15186 16305-16313 VBN denotes obtained
T15187 16314-16317 CC denotes and
T15188 16318-16325 JJ denotes subject
T15189 16326-16328 TO denotes to
T15190 16329-16340 NNS denotes immunoblots
T15191 16341-16345 IN denotes with
T15192 16346-16356 NNS denotes antibodies
T15193 16357-16364 IN denotes against
T15194 16365-16373 NNS denotes proteins
T15195 16374-16382 VBN denotes involved
T15196 16383-16385 IN denotes in
T15197 16386-16391 NNP denotes NF-κB
T15198 16391-16392 , denotes ,
T15199 16393-16402 NNP denotes TBK1/IRF3
T15200 16402-16403 , denotes ,
T15201 16404-16410 NNP denotes ERK1/2
T15202 16410-16411 , denotes ,
T15203 16412-16415 CC denotes and
T15204 16416-16419 CD denotes p38
T15205 16420-16429 VBG denotes signaling
T15206 16430-16438 NNS denotes pathways
T15207 16438-16439 . denotes .
T15208 16440-16450 NN denotes Beta-actin
T15209 16451-16454 VBD denotes was
T15210 16455-16459 VBN denotes used
T15211 16460-16462 IN denotes as
T15212 16463-16470 VBG denotes loading
T15213 16471-16478 NN denotes control
T15214 16478-16479 . denotes .
T15215 16480-16481 -LRB- denotes (
T15216 16481-16482 NNP denotes C
T15217 16482-16483 -RRB- denotes )
T15218 16484-16492 NNP denotes Cellular
T15219 16493-16499 NNS denotes levels
T15220 16500-16502 IN denotes of
T15221 16503-16509 JJ denotes p-IKK1
T15222 16509-16510 NN denotes /
T15223 16510-16511 CD denotes 2
T15224 16512-16515 CC denotes and
T15225 16516-16522 NNP denotes p-IκBα
T15226 16523-16525 IN denotes in
T15227 16526-16529 NNP denotes LPS
T15228 16529-16530 : denotes -
T15229 16531-16533 CC denotes or
T15230 16534-16538 VB denotes poly
T15231 16539-16540 PRP denotes I
T15232 16540-16541 : denotes :
T15233 16541-16553 JJ denotes C-stimulated
T15234 16554-16558 NNP denotes BMDC
T15235 16559-16563 VBD denotes were
T15236 16564-16575 VBN denotes quantitated
T15237 16576-16580 IN denotes with
T15238 16581-16587 NNP denotes ImageJ
T15239 16588-16589 -LRB- denotes (
T15240 16589-16592 NNP denotes NIH
T15241 16592-16593 -RRB- denotes )
T15242 16594-16597 CC denotes and
T15243 16598-16607 VBN denotes presented
T15244 16608-16610 IN denotes as
T15245 16611-16616 NN denotes trend
T15246 16617-16622 NNS denotes lines
T15247 16622-16623 . denotes .
T15248 16624-16631 NNS denotes Results
T15249 16632-16635 VBP denotes are
T15250 16636-16650 NN denotes representative
T15251 16651-16653 IN denotes of
T15252 16654-16656 IN denotes at
T15253 16657-16662 JJS denotes least
T15254 16663-16666 CD denotes two
T15255 16667-16678 JJ denotes independent
T15256 16679-16690 NNS denotes experiments
T15257 16690-16691 . denotes .
T15510 18492-18502 VBN denotes Stimulated
T15511 18503-18507 NN denotes cpdm
T15512 18508-18512 NNP denotes BMDC
T15513 18513-18520 VBD denotes induced
T15514 18521-18531 JJ denotes Th2-biased
T15515 18532-18540 NN denotes cytokine
T15516 18541-18551 NN denotes production
T15517 18552-18556 IN denotes from
T15518 18557-18562 NN denotes naïve
T15519 18563-18566 NNP denotes CD4
T15520 18566-18567 NNP denotes +
T15521 18568-18569 NNP denotes T
T15522 18570-18575 NNS denotes cells
T15523 18575-18576 . denotes .
T15524 18577-18579 NNP denotes WT
T15525 18580-18583 CC denotes and
T15526 18584-18588 VB denotes cpdm
T15527 18589-18593 NNP denotes BMDC
T15528 18594-18595 -LRB- denotes (
T15529 18595-18596 CD denotes 5
T15530 18596-18597 CD denotes ×
T15531 18597-18600 CD denotes 104
T15532 18601-18606 NNS denotes cells
T15533 18607-18609 IN denotes in
T15534 18610-18613 CD denotes 0.1
T15535 18614-18616 NNP denotes mL
T15536 18617-18625 JJ denotes complete
T15537 18626-18632 NN denotes medium
T15538 18632-18633 : denotes ;
T15539 18634-18637 NNP denotes MHC
T15540 18638-18647 NN denotes haplotype
T15541 18647-18648 : denotes :
T15542 18649-18653 NN denotes H-2b
T15543 18653-18654 -RRB- denotes )
T15544 18655-18659 VBD denotes were
T15545 18660-18669 VBN denotes incubated
T15546 18670-18674 IN denotes with
T15547 18675-18681 NN denotes medium
T15548 18681-18682 , denotes ,
T15549 18683-18686 CD denotes 100
T15550 18687-18692 NNP denotes ng/mL
T15551 18693-18696 NNP denotes LPS
T15552 18696-18697 , denotes ,
T15553 18698-18700 CD denotes 25
T15554 18701-18703 NN denotes µg
T15555 18703-18704 CD denotes /
T15556 18704-18706 NNP denotes mL
T15557 18707-18711 NN denotes poly
T15558 18712-18713 PRP denotes I
T15559 18713-18714 : denotes :
T15560 18714-18715 NNP denotes C
T15561 18716-18718 CC denotes or
T15562 18719-18720 CD denotes 5
T15563 18721-18723 NN denotes µg
T15564 18723-18724 NN denotes /
T15565 18724-18726 NNP denotes mL
T15566 18727-18734 NNP denotes Pam3CYS
T15567 18734-18735 . denotes .
T15568 18736-18741 IN denotes After
T15569 18742-18744 CD denotes 24
T15570 18745-18750 NNS denotes hours
T15571 18750-18751 , denotes ,
T15572 18752-18757 NNS denotes cells
T15573 18758-18762 VBD denotes were
T15574 18763-18769 VBN denotes washed
T15575 18770-18774 IN denotes with
T15576 18775-18778 NNP denotes PBS
T15577 18779-18782 CC denotes and
T15578 18783-18792 VBN denotes incubated
T15579 18793-18797 IN denotes with
T15580 18798-18805 RB denotes freshly
T15581 18806-18814 VBN denotes isolated
T15582 18815-18825 JJ denotes allogeneic
T15583 18826-18831 NN denotes naïve
T15584 18832-18835 NNP denotes CD4
T15585 18835-18836 NNP denotes +
T15586 18837-18838 NNP denotes T
T15587 18839-18844 NNS denotes cells
T15588 18845-18846 -LRB- denotes (
T15589 18846-18849 CD denotes 2.5
T15590 18849-18850 CD denotes ×
T15591 18850-18853 CD denotes 105
T15592 18854-18859 NNS denotes cells
T15593 18860-18862 IN denotes in
T15594 18863-18866 CD denotes 0.1
T15595 18867-18869 NNP denotes mL
T15596 18870-18878 JJ denotes complete
T15597 18879-18885 NN denotes medium
T15598 18885-18886 : denotes ;
T15599 18887-18890 NNP denotes MHC
T15600 18891-18900 NN denotes haplotype
T15601 18900-18901 : denotes :
T15602 18902-18906 NN denotes H-2d
T15603 18906-18907 -RRB- denotes )
T15616 18972-18976 NNP denotes IFNγ
T15617 18976-18977 , denotes ,
T15618 18978-18981 NNP denotes IL4
T15619 18981-18982 , denotes ,
T15620 18983-18986 CC denotes and
T15621 18987-18990 NNP denotes IL2
T15622 18991-18995 VBD denotes were
T15623 18996-19004 VBN denotes measured
T15624 19005-19007 IN denotes by
T15625 19008-19013 NNP denotes ELISA
T15626 19013-19014 . denotes .
T15627 19015-19023 JJ denotes Negative
T15628 19024-19032 NNS denotes controls
T15629 19033-19036 VBP denotes are
T15630 19037-19038 CD denotes 1
T15631 19038-19039 -RRB- denotes )
T15632 19040-19050 VBD denotes stimulated
T15633 19051-19055 NNP denotes BMDC
T15634 19056-19063 IN denotes without
T15635 19064-19074 NN denotes co-culture
T15636 19075-19079 IN denotes with
T15637 19080-19090 JJ denotes allogeneic
T15638 19091-19094 NNP denotes CD4
T15639 19094-19095 CD denotes +
T15640 19096-19097 NNP denotes T
T15641 19098-19103 NNS denotes cells
T15642 19103-19104 : denotes ;
T15643 19105-19106 LS denotes 2
T15644 19106-19107 -RRB- denotes )
T15645 19108-19118 JJ denotes allogeneic
T15646 19119-19122 NNP denotes CD4
T15647 19122-19123 CD denotes +
T15648 19124-19125 NNP denotes T
T15649 19126-19131 NNS denotes cells
T15650 19132-19139 IN denotes without
T15651 19140-19150 NN denotes co-culture
T15652 19151-19155 IN denotes with
T15653 19156-19166 VBN denotes stimulated
T15654 19167-19171 NNP denotes BMDC
T15655 19171-19172 . denotes .
T15656 19173-19180 NNS denotes Samples
T15657 19181-19185 IN denotes from
T15658 19186-19190 DT denotes both
T15659 19191-19199 JJ denotes negative
T15660 19200-19208 NNS denotes controls
T15661 19209-19212 VBD denotes had
T15662 19213-19215 DT denotes no
T15663 19216-19226 JJ denotes detectable
T15664 19227-19237 NN denotes production
T15665 19238-19240 IN denotes of
T15666 19241-19244 DT denotes the
T15667 19245-19259 JJ denotes aforementioned
T15668 19260-19269 NNS denotes cytokines
T15669 19270-19271 -LRB- denotes (
T15670 19271-19274 RB denotes not
T15671 19275-19280 VBN denotes shown
T15672 19280-19281 -RRB- denotes )
T15673 19281-19282 . denotes .
T15674 19283-19290 NNS denotes Results
T15675 19291-19294 VBP denotes are
T15676 19295-19303 VBN denotes analyzed
T15677 19304-19309 VBN denotes based
T15678 19310-19312 IN denotes on
T15679 19313-19314 CD denotes 2
T15680 19315-19316 CD denotes 4
T15681 19317-19321 NNS denotes mice
T15682 19322-19325 IN denotes per
T15683 19326-19331 NN denotes group
T15684 19331-19332 . denotes .
T15685 19333-19334 SYM denotes *
T15686 19335-19336 NN denotes P
T15687 19336-19337 NN denotes <
T15688 19337-19341 CD denotes 0.05
T15689 19341-19342 . denotes .
T995 3821-3830 VBN denotes Activated
T996 3831-3834 CC denotes and
T997 3835-3841 VBP denotes mature
T998 3842-3844 NNP denotes DC
T999 3845-3848 MD denotes can
T1000 3849-3854 VB denotes carry
T1001 3855-3864 VBN denotes processed
T1002 3865-3874 JJ denotes antigenic
T1003 3875-3883 NNS denotes peptides
T1004 3883-3884 , denotes ,
T1005 3885-3892 VB denotes migrate
T1006 3893-3895 TO denotes to
T1007 3896-3904 JJ denotes lymphoid
T1008 3905-3911 NNS denotes organs
T1009 3911-3912 , denotes ,
T1010 3913-3916 CC denotes and
T1011 3917-3923 VB denotes induce
T1012 3924-3939 JJ denotes T-cell-mediated
T1056 4202-4211 NN denotes phenotype
T1057 4212-4214 IN denotes in
T1058 4215-4232 JJ denotes SHARPIN-deficient
T1059 4233-4237 NNS denotes mice
T1060 4237-4238 . denotes .
T1061 4239-4242 DT denotes The
T1062 4243-4250 NNS denotes studies
T1063 4251-4259 VBD denotes reported
T1064 4260-4264 RB denotes here
T1065 4265-4270 VBN denotes found
T1066 4271-4275 IN denotes that
T1067 4276-4280 NN denotes lack
T1068 4281-4283 IN denotes of
T1069 4284-4291 NNP denotes SHARPIN
T1070 4292-4299 NN denotes protein
T1071 4300-4302 IN denotes in
T1072 4303-4307 NNP denotes BMDC
T1073 4308-4314 VBD denotes caused
T1074 4315-4324 JJ denotes defective
T1075 4325-4335 NN denotes expression
T1076 4336-4338 IN denotes of
T1077 4339-4355 JJ denotes pro-inflammatory
T1078 4356-4365 NNS denotes mediators
T1079 4366-4369 CC denotes and
T1080 4370-4378 VBN denotes impaired
T1081 4379-4384 JJ denotes NF-κB
T1082 4385-4395 NN denotes activation
T1083 4396-4400 IN denotes upon
T1084 4401-4407 NN denotes ligand
T1085 4408-4419 NN denotes stimulation
T1086 4419-4420 . denotes .
T1087 4421-4424 DT denotes The
T1088 4425-4432 NN denotes ability
T1089 4433-4435 IN denotes of
T1090 4436-4440 NN denotes cpdm
T1091 4441-4445 NNP denotes BMDC
T1092 4446-4448 TO denotes to
T1093 4449-4458 VB denotes stimulate
T1094 4459-4462 JJ denotes Th1
T1095 4463-4471 NN denotes cytokine
T1096 4472-4482 NN denotes production
T1097 4483-4485 IN denotes in
T1098 4486-4496 JJ denotes allogeneic
T1099 4497-4500 NNP denotes CD4
T1100 4500-4501 CD denotes +
T1101 4502-4503 NNP denotes T
T1102 4504-4509 NNS denotes cells
T1103 4510-4513 VBD denotes was
T1104 4514-4525 VBN denotes compromised
T1105 4525-4526 . denotes .
T1106 4527-4532 VBN denotes Taken
T1107 4533-4541 RB denotes together
T1108 4541-4542 , denotes ,
T1109 4543-4548 DT denotes these
T1110 4549-4556 NNS denotes results
T1111 4557-4563 VBP denotes reveal
T1112 4564-4568 IN denotes that
T1113 4569-4576 NNP denotes SHARPIN
T1114 4577-4579 VBZ denotes is
T118 0-7 NNP denotes SHARPIN
T119 8-10 VBZ denotes Is
T120 11-20 JJ denotes Essential
T121 21-24 IN denotes for
T122 25-33 NNP denotes Cytokine
T123 34-44 NNP denotes Production
T124 44-45 , denotes ,
T125 46-51 NNP denotes NF-κB
T126 52-61 NNP denotes Signaling
T14843 14803-14806 CD denotes 105
T14844 14807-14812 NNS denotes cells
T14845 14813-14815 IN denotes in
T14846 14816-14819 CD denotes 0.2
T14847 14820-14822 NN denotes ml
T14848 14823-14831 JJ denotes complete
T14849 14832-14838 NN denotes medium
T14850 14838-14839 -RRB- denotes )
T14851 14840-14844 VBD denotes were
T14852 14845-14851 VBN denotes washed
T15604 18907-18908 . denotes .
T15605 18909-18914 IN denotes After
T15606 18915-18916 CD denotes 5
T15607 18917-18921 NNS denotes days
T15608 18921-18922 , denotes ,
T15609 18923-18935 NNS denotes supernatants
T15610 18936-18940 VBD denotes were
T15611 18941-18950 VBN denotes collected
T15612 18951-18954 CC denotes and
T15613 18955-18958 DT denotes the
T15614 18959-18968 NN denotes secretion
T15615 18969-18971 IN denotes of
R71 T155 T154 prep in,gene
R72 T156 T155 pobj mice,in
R73 T157 T119 conj result,Is
R74 T158 T157 prep in,result
R75 T159 T160 amod systemic,inflammation
R76 T160 T158 pobj inflammation,in
R77 T161 T163 nsubjpass that,characterized
R78 T162 T163 auxpass is,characterized
R79 T163 T160 relcl characterized,inflammation
R80 T164 T163 agent by,characterized
R81 T165 T167 amod chronic,dermatitis
R82 T166 T167 compound proliferative,dermatitis
R83 T167 T164 pobj dermatitis,by
R84 T168 T167 cc and,dermatitis
R85 T169 T170 amod dysregulated,secretion
R86 T170 T167 conj secretion,dermatitis
R87 T171 T170 prep of,secretion
R88 T172 T173 compound T,helper1
R89 T173 T171 pobj helper1,of
R90 T174 T175 punct (,Th1
R91 T175 T170 appos Th1,secretion
R92 T176 T175 punct ),Th1
R93 T177 T170 cc and,secretion
R94 T178 T179 amod Th2,cytokines
R95 T179 T170 conj cytokines,secretion
R96 T180 T157 punct .,result
R97 T181 T185 det The,mechanisms
R98 T182 T185 amod cellular,mechanisms
R99 T183 T182 cc and,cellular
R100 T184 T182 conj molecular,cellular
R101 T185 T190 nsubj mechanisms,remain
R102 T186 T185 acl underlying,mechanisms
R103 T187 T189 det this,phenotype
R104 T188 T189 amod inflammatory,phenotype
R105 T189 T190 nsubj phenotype,remain
R106 T190 T190 ROOT remain,remain
R107 T191 T190 acomp elusive,remain
R108 T192 T190 punct .,remain
R109 T193 T194 amod Dendritic,cells
R110 T194 T196 nsubj cells,contribute
R111 T195 T196 aux may,contribute
R112 T196 T196 ROOT contribute,contribute
R113 T197 T196 prep to,contribute
R114 T198 T199 det the,initiation
R115 T199 T197 pobj initiation,to
R116 T200 T199 cc and,initiation
R117 T201 T199 conj progression,initiation
R118 T202 T199 prep of,initiation
R119 T203 T204 det the,phenotype
R120 T204 T202 pobj phenotype,of
R121 T205 T204 prep of,phenotype
R122 T206 T207 amod SHARPIN-deficient,mice
R123 T207 T205 pobj mice,of
R124 T208 T196 prep because,contribute
R125 T209 T208 pcomp of,because
R126 T210 T212 poss their,role
R127 T211 T212 amod pivotal,role
R128 T212 T208 pobj role,because
R129 T213 T212 prep in,role
R130 T214 T217 amod innate,immunity
R131 T215 T214 cc and,innate
R132 T216 T214 conj adaptive,innate
R133 T217 T213 pobj immunity,in
R134 T218 T196 punct .,contribute
R135 T219 T221 advmod Here,show
R136 T220 T221 nsubj we,show
R137 T221 T221 ROOT show,show
R138 T222 T221 prep by,show
R139 T223 T222 pobj flow,by
R140 T224 T221 dobj cytometry,show
R141 T225 T231 mark that,alter
R142 T226 T228 compound SHARPIN,deficiency
R143 T227 T228 punct -,deficiency
R144 T228 T231 nsubj deficiency,alter
R145 T229 T231 aux did,alter
R146 T230 T231 neg not,alter
R147 T231 T221 ccomp alter,show
R148 T232 T233 det the,distribution
R149 T233 T231 dobj distribution,alter
R150 T234 T233 prep of,distribution
R151 T235 T237 amod different,subtypes
R152 T236 T237 compound DC,subtypes
R153 T237 T234 pobj subtypes,of
R154 T238 T233 prep in,distribution
R155 T239 T240 det the,spleen
R156 T240 T238 pobj spleen,in
R157 T241 T221 punct .,show
R158 T242 T250 nmod In,agonists
R159 T243 T242 pobj response,In
R160 T244 T243 prep to,response
R161 T245 T246 amod TOLL-like,receptor
R162 T246 T244 pobj receptor,to
R163 T247 T248 punct (,TLR
R164 T248 T246 appos TLR,receptor
R165 T249 T242 punct ),In
R166 T250 T250 ROOT agonists,agonists
R167 T251 T250 dobj LPS,agonists
R168 T252 T250 cc and,agonists
R169 T253 T258 advmod poly,cultured
R170 T254 T258 nsubj I,cultured
R171 T255 T256 punct :,C
R172 T256 T258 npadvmod C,cultured
R173 T257 T258 punct ",",cultured
R174 T258 T250 conj cultured,agonists
R175 T259 T262 nmod bone,cells
R176 T260 T262 amod marrow-derived,cells
R177 T261 T262 amod dendritic,cells
R178 T262 T258 dobj cells,cultured
R179 T263 T264 punct (,BMDC
R180 T264 T262 appos BMDC,cells
R181 T265 T264 punct ),BMDC
R182 T266 T264 prep from,BMDC
R183 T267 T266 pobj WT,from
R184 T268 T264 cc and,BMDC
R185 T269 T270 amod mutant,mice
R186 T270 T264 conj mice,BMDC
R187 T271 T258 conj exhibited,cultured
R188 T272 T273 amod similar,increases
R189 T273 T271 dobj increases,exhibited
R190 T274 T273 prep in,increases
R191 T275 T274 pobj expression,in
R192 T276 T275 prep of,expression
R193 T277 T278 amod co-stimulatory,molecules
R194 T278 T276 pobj molecules,of
R195 T279 T273 conj CD40,increases
R196 T280 T279 punct ",",CD40
R197 T281 T279 conj CD80,CD40
R198 T282 T281 punct ",",CD80
R199 T283 T281 cc and,CD80
R200 T284 T281 conj CD86,CD80
R201 T285 T258 punct .,cultured
R202 T286 T288 advmod However,stimulated
R203 T287 T288 punct ",",stimulated
R204 T288 T288 ROOT stimulated,stimulated
R205 T289 T290 compound SHARPIN-deficient,BMDC
R206 T290 T292 nsubj BMDC,reduced
R207 T291 T292 aux had,reduced
R208 T292 T288 ccomp reduced,stimulated
R209 T293 T292 dobj transcription,reduced
R210 T294 T293 cc and,transcription
R211 T295 T293 conj secretion,transcription
R212 T296 T293 prep of,transcription
R213 T297 T298 amod pro-inflammatory,mediators
R214 T298 T296 pobj mediators,of
R215 T299 T292 dobj IL6,reduced
R216 T300 T299 punct ",",IL6
R217 T301 T299 conj IL12P70,IL6
R218 T302 T301 punct ",",IL12P70
R219 T303 T301 conj GMCSF,IL12P70
R220 T304 T303 punct ",",GMCSF
R221 T305 T303 cc and,GMCSF
R222 T306 T307 amod nitric,oxide
R223 T307 T303 conj oxide,GMCSF
R224 T308 T292 punct .,reduced
R225 T309 T310 compound Mutant,BMDC
R226 T310 T311 nsubj BMDC,had
R227 T311 T311 ROOT had,had
R228 T312 T313 amod defective,activation
R229 T313 T311 dobj activation,had
R230 T314 T313 prep of,activation
R231 T315 T316 amod NF-κB,signaling
R232 T316 T314 pobj signaling,of
R233 T317 T311 punct ",",had
R234 T318 T338 mark whereas,were
R235 T319 T320 det the,MAPK1/3
R236 T320 T338 nsubj MAPK1/3,were
R237 T321 T322 punct (,ERK1/2
R238 T322 T320 appos ERK1/2,MAPK1/3
R239 T323 T322 punct ),ERK1/2
R240 T324 T320 cc and,MAPK1/3
R241 T325 T326 compound MAPK11/12/13,/
R242 T326 T338 nsubj /,were
R243 T327 T326 nummod 14,/
R244 T328 T332 punct (,isoforms
R245 T329 T331 nummod p38,kinase
R246 T330 T331 compound MAP,kinase
R247 T331 T332 nsubj kinase,isoforms
R248 T332 T326 parataxis isoforms,/
R249 T333 T332 punct ),isoforms
R250 T334 T326 cc and,/
R251 T335 T337 nummod TBK1,pathways
R252 T336 T337 compound signaling,pathways
R253 T337 T326 conj pathways,/
R254 T338 T311 advcl were,had
R255 T339 T338 acomp intact,were
R256 T340 T311 punct .,had
R257 T341 T344 det A,reaction
R258 T342 T344 amod mixed,reaction
R259 T343 T344 compound lymphocyte,reaction
R260 T344 T345 nsubj reaction,showed
R261 T345 T345 ROOT showed,showed
R262 T346 T350 mark that,induced
R263 T347 T348 compound mutant,BMDC
R264 T348 T350 nsubj BMDC,induced
R265 T349 T350 advmod only,induced
R266 T350 T345 ccomp induced,showed
R267 T351 T355 det a,response
R268 T352 T355 amod weak,response
R269 T353 T355 nummod Th1,response
R270 T354 T355 amod immune,response
R271 T355 T350 dobj response,induced
R272 T356 T350 cc but,induced
R273 T357 T350 conj stimulated,induced
R274 T358 T361 amod increased,production
R275 T359 T361 amod Th2,production
R276 T360 T361 compound cytokine,production
R277 T361 T357 dobj production,stimulated
R278 T362 T361 prep from,production
R279 T363 T364 amod allogeneic,naïve
R280 T364 T368 amod naïve,cells
R281 T365 T368 compound CD4,cells
R282 T366 T368 compound +,cells
R283 T367 T368 compound T,cells
R284 T368 T362 pobj cells,from
R285 T369 T345 punct .,showed
R286 T370 T379 prep In,affects
R287 T371 T370 pobj conclusion,In
R288 T372 T379 punct ",",affects
R289 T373 T379 nsubj loss,affects
R290 T374 T373 prep of,loss
R291 T375 T374 pobj Sharpin,of
R292 T376 T373 prep in,loss
R293 T377 T376 pobj mice,in
R294 T378 T379 advmod significantly,affects
R295 T379 T379 ROOT affects,affects
R296 T380 T382 det the,function
R297 T381 T382 amod immune,function
R298 T382 T379 dobj function,affects
R299 T383 T382 prep of,function
R300 T384 T383 pobj DC,of
R301 T385 T379 cc and,affects
R302 T386 T389 nsubj this,account
R303 T387 T389 aux may,account
R304 T388 T389 advmod partially,account
R305 T389 T379 conj account,affects
R306 T390 T389 prep for,account
R307 T391 T393 det the,inflammation
R308 T392 T393 amod systemic,inflammation
R309 T393 T390 pobj inflammation,for
R310 T394 T393 cc and,inflammation
R311 T395 T397 amod Th2-biased,response
R312 T396 T397 amod immune,response
R313 T397 T393 conj response,inflammation
R314 T398 T389 punct .,account
R685 T118 T119 nsubj SHARPIN,Is
R686 T119 T119 ROOT Is,Is
R687 T120 T119 acomp Essential,Is
R688 T121 T119 prep for,Is
R689 T122 T123 compound Cytokine,Production
R690 T123 T121 pobj Production,for
R691 T124 T123 punct ",",Production
R692 T125 T126 compound NF-κB,Signaling
R693 T126 T123 conj Signaling,Production
R757 T995 T1000 advcl Activated,carry
R758 T996 T995 cc and,Activated
R759 T997 T995 conj mature,Activated
R760 T998 T1000 nsubj DC,carry
R761 T999 T1000 aux can,carry
R762 T1000 T1000 ROOT carry,carry
R763 T1001 T1003 amod processed,peptides
R764 T1002 T1003 amod antigenic,peptides
R765 T1003 T1000 dobj peptides,carry
R766 T1004 T1000 punct ",",carry
R767 T1005 T1000 conj migrate,carry
R768 T1006 T1005 prep to,migrate
R769 T1007 T1008 amod lymphoid,organs
R770 T1008 T1006 pobj organs,to
R771 T1009 T1005 punct ",",migrate
R772 T1010 T1005 cc and,migrate
R773 T1011 T1005 conj induce,migrate
R774 T1012 T1014 amod T-cell-mediated,responses
R775 T1013 T1014 amod immune,responses
R776 T1014 T1011 dobj responses,induce
R777 T1015 T1014 cc or,responses
R778 T1016 T1014 conj tolerance,responses
R779 T1017 T1000 punct .,carry
R780 T1018 T1018 ROOT DC,DC
R781 T1019 T1036 amod direct,through
R782 T1020 T1021 det the,differentiation
R783 T1021 T1019 dobj differentiation,direct
R784 T1022 T1021 prep of,differentiation
R785 T1023 T1026 nummod CD4,cells
R786 T1024 T1026 nummod +,cells
R787 T1025 T1026 compound T,cells
R788 T1026 T1022 pobj cells,of
R789 T1027 T1021 punct ",",differentiation
R790 T1028 T1021 cc and,differentiation
R791 T1029 T1031 advmod hence,type
R792 T1030 T1031 det the,type
R793 T1031 T1021 conj type,differentiation
R794 T1032 T1031 prep of,type
R795 T1033 T1034 amod immune,response
R796 T1034 T1032 pobj response,of
R797 T1035 T1031 punct ",",type
R798 T1036 T1018 prep through,DC
R799 T1037 T1039 det the,secretion
R800 T1038 T1039 amod selective,secretion
R801 T1039 T1036 pobj secretion,through
R802 T1040 T1039 prep of,secretion
R803 T1041 T1040 pobj cytokines,of
R804 T1042 T1018 punct .,DC
R805 T1043 T1044 nsubj We,hypothesized
R806 T1044 T1044 ROOT hypothesized,hypothesized
R807 T1045 T1051 mark that,contributed
R808 T1046 T1048 amod defective,secretion
R809 T1047 T1048 compound cytokine,secretion
R810 T1048 T1051 nsubj secretion,contributed
R811 T1049 T1048 prep by,secretion
R812 T1050 T1049 pobj DC,by
R813 T1051 T1044 ccomp contributed,hypothesized
R814 T1052 T1051 prep to,contributed
R815 T1053 T1056 det the,phenotype
R816 T1054 T1056 amod Th2-biased,phenotype
R817 T1055 T1056 amod inflammatory,phenotype
R818 T1056 T1052 pobj phenotype,to
R819 T1057 T1056 prep in,phenotype
R820 T1058 T1059 amod SHARPIN-deficient,mice
R821 T1059 T1057 pobj mice,in
R822 T1060 T1044 punct .,hypothesized
R823 T1061 T1062 det The,studies
R824 T1062 T1065 nsubj studies,found
R825 T1063 T1062 acl reported,studies
R826 T1064 T1063 advmod here,reported
R827 T1065 T1065 ROOT found,found
R828 T1066 T1073 mark that,caused
R829 T1067 T1073 nsubj lack,caused
R830 T1068 T1067 prep of,lack
R831 T1069 T1070 compound SHARPIN,protein
R832 T1070 T1068 pobj protein,of
R833 T1071 T1067 prep in,lack
R834 T1072 T1071 pobj BMDC,in
R835 T1073 T1065 ccomp caused,found
R836 T1074 T1075 amod defective,expression
R837 T1075 T1073 dobj expression,caused
R838 T1076 T1075 prep of,expression
R839 T1077 T1078 amod pro-inflammatory,mediators
R840 T1078 T1076 pobj mediators,of
R841 T1079 T1075 cc and,expression
R842 T1080 T1082 amod impaired,activation
R843 T1081 T1082 amod NF-κB,activation
R844 T1082 T1075 conj activation,expression
R845 T1083 T1082 prep upon,activation
R846 T1084 T1085 amod ligand,stimulation
R847 T1085 T1083 pobj stimulation,upon
R848 T1086 T1065 punct .,found
R849 T1087 T1088 det The,ability
R850 T1088 T1104 nsubjpass ability,compromised
R851 T1089 T1088 prep of,ability
R852 T1090 T1091 compound cpdm,BMDC
R853 T1091 T1089 pobj BMDC,of
R854 T1092 T1093 aux to,stimulate
R855 T1093 T1088 acl stimulate,ability
R856 T1094 T1096 amod Th1,production
R857 T1095 T1096 compound cytokine,production
R858 T1096 T1093 dobj production,stimulate
R859 T1097 T1096 prep in,production
R860 T1098 T1099 amod allogeneic,CD4
R861 T1099 T1101 compound CD4,T
R862 T1100 T1101 compound +,T
R863 T1101 T1102 compound T,cells
R864 T1102 T1097 pobj cells,in
R865 T1103 T1104 auxpass was,compromised
R866 T1104 T1104 ROOT compromised,compromised
R867 T1105 T1104 punct .,compromised
R868 T1106 T1111 advcl Taken,reveal
R869 T1107 T1106 advmod together,Taken
R870 T1108 T1111 punct ",",reveal
R871 T1109 T1110 det these,results
R872 T1110 T1111 nsubj results,reveal
R873 T1111 T1111 ROOT reveal,reveal
R874 T1112 T1114 mark that,is
R875 T1113 T1114 nsubj SHARPIN,is
R876 T1114 T1111 ccomp is,reveal
R877 T1115 T1465 det a,molecule
R878 T1116 T1465 amod novel,molecule
R879 T1117 T1120 nsubjpass SHARPIN,identified
R880 T1118 T1120 auxpass was,identified
R881 T1119 T1120 advmod originally,identified
R882 T1120 T1120 ROOT identified,identified
R883 T1121 T1120 prep in,identified
R884 T1122 T1123 amod post-synaptic,densities
R885 T1123 T1121 pobj densities,in
R886 T1124 T1123 prep of,densities
R887 T1125 T1126 amod excitatory,synapses
R888 T1126 T1124 pobj synapses,of
R889 T1127 T1120 prep in,identified
R890 T1128 T1129 det the,brain
R891 T1129 T1127 pobj brain,in
R892 T1130 T1129 prep of,brain
R893 T1131 T1130 pobj rats,of
R894 T1132 T1134 nmod [,]
R895 T1133 T1134 nummod 1,]
R896 T1134 T1134 ROOT ],]
R897 T1135 T1134 punct ",",]
R898 T1136 T1141 cc but,expressed
R899 T1137 T1138 det this,protein
R900 T1138 T1141 nsubjpass protein,expressed
R901 T1139 T1141 auxpass is,expressed
R902 T1140 T1141 advmod widely,expressed
R903 T1141 T1134 parataxis expressed,]
R904 T1142 T1141 prep in,expressed
R905 T1143 T1144 det a,variety
R906 T1144 T1142 pobj variety,in
R907 T1145 T1144 prep of,variety
R908 T1146 T1145 pobj tissues,of
R909 T1147 T1149 nmod [,]
R910 T1148 T1149 nummod 2,]
R911 T1149 T1144 appos ],variety
R912 T1150 T1141 punct .,expressed
R913 T1151 T1156 nummod Two,mutations
R914 T1152 T1156 amod allelic,mutations
R915 T1153 T1156 punct ",",mutations
R916 T1154 T1156 amod autosomal,mutations
R917 T1155 T1156 amod recessive,mutations
R918 T1156 T1161 nsubj mutations,occurred
R919 T1157 T1156 prep in,mutations
R920 T1158 T1160 det the,gene
R921 T1159 T1160 compound Sharpin,gene
R922 T1160 T1157 pobj gene,in
R923 T1161 T1161 ROOT occurred,occurred
R924 T1162 T1161 advmod spontaneously,occurred
R925 T1163 T1161 prep in,occurred
R926 T1164 T1166 nummod two,strains
R927 T1165 T1166 amod inbred,strains
R928 T1166 T1163 pobj strains,in
R929 T1167 T1166 prep of,strains
R930 T1168 T1167 pobj mice,of
R931 T1169 T1166 punct ",",strains
R932 T1170 T1166 appos C57B/KaLawRij-Sharpincpdm/Sharpincpdm,strains
R933 T1171 T1170 cc and,C57B/KaLawRij-Sharpincpdm/Sharpincpdm
R934 T1172 T1174 amod CBy.Ocb3,Dem-Sharpincpdm-Dem/Sharpincpdm-Dem
R935 T1173 T1174 compound /,Dem-Sharpincpdm-Dem/Sharpincpdm-Dem
R936 T1174 T1170 conj Dem-Sharpincpdm-Dem/Sharpincpdm-Dem,C57B/KaLawRij-Sharpincpdm/Sharpincpdm
R937 T1175 T1166 punct ",",strains
R938 T1176 T1161 advcl resulting,occurred
R939 T1177 T1176 prep in,resulting
R940 T1178 T1179 amod premature,termination
R941 T1179 T1177 pobj termination,in
R942 T1180 T1179 prep of,termination
R943 T1181 T1182 compound mRNA,synthesis
R944 T1182 T1180 pobj synthesis,of
R945 T1183 T1182 cc and,synthesis
R946 T1184 T1182 conj absence,synthesis
R947 T1185 T1184 prep of,absence
R948 T1186 T1189 det a,product
R949 T1187 T1188 amod functional,protein
R950 T1188 T1189 compound protein,product
R951 T1189 T1185 pobj product,of
R952 T1190 T1192 nmod [,]
R953 T1191 T1192 nummod 2,]
R954 T1192 T1176 dobj ],resulting
R955 T1193 T1161 punct .,occurred
R956 T1194 T1201 prep Despite,cause
R957 T1195 T1197 amod different,backgrounds
R958 T1196 T1197 amod genetic,backgrounds
R959 T1197 T1194 pobj backgrounds,Despite
R960 T1198 T1201 punct ",",cause
R961 T1199 T1200 det both,mutations
R962 T1200 T1201 nsubj mutations,cause
R963 T1201 T1201 ROOT cause,cause
R964 T1202 T1204 amod similar,disease
R965 T1203 T1204 amod inflammatory,disease
R966 T1204 T1201 dobj disease,cause
R967 T1205 T1201 prep with,cause
R968 T1206 T1209 amod severe,dermatitis
R969 T1207 T1209 amod chronic,dermatitis
R970 T1208 T1209 amod progressive,dermatitis
R971 T1209 T1205 pobj dermatitis,with
R972 T1210 T1209 cc and,dermatitis
R973 T1211 T1212 amod defective,development
R974 T1212 T1209 conj development,dermatitis
R975 T1213 T1212 prep of,development
R976 T1214 T1216 amod secondary,organs
R977 T1215 T1216 amod lymphoid,organs
R978 T1216 T1213 pobj organs,of
R979 T1217 T1219 nmod [,]
R980 T1218 T1219 nummod 2,]
R981 T1219 T1220 meta ],[
R982 T1220 T1222 nmod [,]
R983 T1221 T1222 nummod 4,]
R984 T1222 T1201 npadvmod ],cause
R985 T1223 T1201 punct .,cause
R986 T1224 T1225 det The,dermatitis
R987 T1225 T1226 nsubj dermatitis,becomes
R988 T1226 T1226 ROOT becomes,becomes
R989 T1227 T1228 advmod clinically,apparent
R990 T1228 T1226 acomp apparent,becomes
R991 T1229 T1226 prep at,becomes
R992 T1230 T1231 quantmod about,four
R993 T1231 T1232 nummod four,weeks
R994 T1232 T1229 pobj weeks,at
R995 T1233 T1232 prep of,weeks
R996 T1234 T1233 pobj age,of
R997 T1235 T1226 punct .,becomes
R998 T1236 T1237 expl There,are
R999 T1237 T1237 ROOT are,are
R1000 T1238 T1237 attr accumulations,are
R1001 T1239 T1238 prep of,accumulations
R1002 T1240 T1239 pobj eosinophils,of
R1003 T1241 T1240 punct ",",eosinophils
R1004 T1242 T1240 conj neutrophils,eosinophils
R1005 T1243 T1242 cc and,neutrophils
R1006 T1244 T1242 conj macrophages,neutrophils
R1007 T1245 T1238 prep in,accumulations
R1008 T1246 T1247 det the,skin
R1009 T1247 T1245 pobj skin,in
R1010 T1248 T1247 prep of,skin
R1011 T1249 T1251 nmod Sharpincpdm/Sharpincpdm,mice
R1012 T1250 T1251 amod mutant,mice
R1013 T1251 T1248 pobj mice,of
R1014 T1252 T1254 punct (,referred
R1015 T1253 T1254 nsubj hereafter,referred
R1016 T1254 T1237 parataxis referred,are
R1017 T1255 T1254 prep to,referred
R1018 T1256 T1254 prep as,referred
R1019 T1257 T1258 compound cpdm,mice
R1020 T1258 T1256 pobj mice,as
R1021 T1259 T1254 punct ),referred
R1022 T1260 T1237 conj associated,are
R1023 T1261 T1260 prep with,associated
R1024 T1262 T1263 amod increased,expression
R1025 T1263 T1261 pobj expression,with
R1026 T1264 T1263 prep of,expression
R1027 T1265 T1266 amod Th2,cytokines
R1028 T1266 T1264 pobj cytokines,of
R1029 T1267 T1266 prep in,cytokines
R1030 T1268 T1269 det the,skin
R1031 T1269 T1267 pobj skin,in
R1032 T1270 T1267 cc and,in
R1033 T1271 T1267 conj in,in
R1034 T1272 T1273 det the,supernatants
R1035 T1273 T1271 pobj supernatants,in
R1036 T1274 T1273 prep of,supernatants
R1037 T1275 T1276 amod activated,splenocytes
R1038 T1276 T1274 pobj splenocytes,of
R1039 T1277 T1279 nmod [,]
R1040 T1278 T1279 nummod 5,]
R1041 T1279 T1260 npadvmod ],associated
R1042 T1280 T1260 punct .,associated
R1043 T1281 T1282 det The,mice
R1044 T1282 T1283 nsubj mice,have
R1045 T1283 T1283 ROOT have,have
R1046 T1284 T1287 det an,type
R1047 T1285 T1287 amod impaired,type
R1048 T1286 T1287 amod delayed,type
R1049 T1287 T1283 dobj type,have
R1050 T1288 T1289 amod hypersensitivity,response
R1051 T1289 T1287 dobj response,type
R1052 T1290 T1283 cc and,have
R1053 T1291 T1283 conj decreased,have
R1054 T1292 T1291 dobj secretion,decreased
R1055 T1293 T1292 prep of,secretion
R1056 T1294 T1295 compound IFNγ,[
R1057 T1295 T1297 nmod [,]
R1058 T1296 T1297 nummod 5,]
R1059 T1297 T1293 pobj ],of
R1060 T1298 T1291 punct ",",decreased
R1061 T1299 T1291 advcl indicating,decreased
R1062 T1300 T1301 det a,defect
R1063 T1301 T1299 dobj defect,indicating
R1064 T1302 T1301 prep in,defect
R1065 T1303 T1305 nummod Th1,responses
R1066 T1304 T1305 amod immune,responses
R1067 T1305 T1302 pobj responses,in
R1068 T1306 T1305 cc and,responses
R1069 T1307 T1308 det a,bias
R1070 T1308 T1305 conj bias,responses
R1071 T1309 T1308 prep towards,bias
R1072 T1310 T1313 det a,response
R1073 T1311 T1313 amod Th2,response
R1074 T1312 T1313 amod immune,response
R1075 T1313 T1309 pobj response,towards
R1076 T1314 T1283 punct .,have
R1077 T1315 T1316 amod Systemic,treatment
R1078 T1316 T1323 nsubj treatment,caused
R1079 T1317 T1316 prep of,treatment
R1080 T1318 T1319 compound cpdm,mice
R1081 T1319 T1317 pobj mice,of
R1082 T1320 T1316 prep with,treatment
R1083 T1321 T1322 amod recombinant,IL12
R1084 T1322 T1320 pobj IL12,with
R1085 T1323 T1323 ROOT caused,caused
R1086 T1324 T1325 amod complete,remission
R1087 T1325 T1323 dobj remission,caused
R1088 T1326 T1325 prep of,remission
R1089 T1327 T1329 det the,[
R1090 T1328 T1329 compound dermatitis,[
R1091 T1329 T1326 pobj [,of
R1092 T1330 T1329 nummod 5,[
R1093 T1331 T1329 appos ],[
R1094 T1332 T1323 punct .,caused
R1095 T1333 T1344 nsubj Neutralization,reduced
R1096 T1334 T1333 prep of,Neutralization
R1097 T1335 T1334 pobj IL5,of
R1098 T1336 T1333 prep by,Neutralization
R1099 T1337 T1338 compound antibody,treatment
R1100 T1338 T1336 pobj treatment,by
R1101 T1339 T1338 cc or,treatment
R1102 T1340 T1338 conj crosses,treatment
R1103 T1341 T1333 prep with,Neutralization
R1104 T1342 T1343 amod IL5-deficient,mice
R1105 T1343 T1341 pobj mice,with
R1106 T1344 T1344 ROOT reduced,reduced
R1107 T1345 T1346 det the,number
R1108 T1346 T1344 dobj number,reduced
R1109 T1347 T1346 prep of,number
R1110 T1348 T1347 pobj circulating,of
R1111 T1349 T1348 cc and,circulating
R1112 T1350 T1348 conj cutaneous,circulating
R1113 T1351 T1348 conj eosinophils,circulating
R1114 T1352 T1344 punct ",",reduced
R1115 T1353 T1344 cc but,reduced
R1116 T1354 T1344 conj failed,reduced
R1117 T1355 T1356 aux to,reduce
R1118 T1356 T1354 xcomp reduce,failed
R1119 T1357 T1358 det the,onset
R1120 T1358 T1356 dobj onset,reduce
R1121 T1359 T1358 cc and,onset
R1122 T1360 T1358 conj severity,onset
R1123 T1361 T1358 prep of,onset
R1124 T1362 T1364 det the,[
R1125 T1363 T1364 compound dermatitis,[
R1126 T1364 T1361 pobj [,of
R1127 T1365 T1364 nummod 6,[
R1128 T1366 T1364 appos ],[
R1129 T1367 T1344 punct .,reduced
R1130 T1368 T1373 advmod Recently,identified
R1131 T1369 T1373 punct ",",identified
R1132 T1370 T1372 nummod three,groups
R1133 T1371 T1372 amod independent,groups
R1134 T1372 T1373 nsubj groups,identified
R1135 T1373 T1373 ROOT identified,identified
R1136 T1374 T1373 dobj SHARPIN,identified
R1137 T1375 T1373 prep as,identified
R1138 T1376 T1378 det an,component
R1139 T1377 T1378 amod essential,component
R1140 T1378 T1375 pobj component,as
R1141 T1379 T1378 prep of,component
R1142 T1380 T1385 det the,complex
R1143 T1381 T1385 nmod linear,complex
R1144 T1382 T1385 compound ubiquitin,complex
R1145 T1383 T1385 compound chain,complex
R1146 T1384 T1385 compound assembly,complex
R1147 T1385 T1379 pobj complex,of
R1148 T1386 T1387 punct (,LUBAC
R1149 T1387 T1385 appos LUBAC,complex
R1150 T1388 T1385 punct ),complex
R1151 T1389 T1390 nsubj that,regulates
R1152 T1390 T1378 relcl regulates,component
R1153 T1391 T1393 amod TNFα-induced,NF-κB
R1154 T1392 T1393 amod canonical,NF-κB
R1155 T1393 T1390 dobj NF-κB,regulates
R1156 T1394 T1393 acl signaling,NF-κB
R1157 T1395 T1397 nmod [,]
R1158 T1396 T1397 nummod 7,]
R1159 T1397 T1394 dobj ],signaling
R1160 T1398 T1397 appos [,]
R1161 T1399 T1400 nummod 9,]
R1162 T1400 T1397 conj ],]
R1163 T1401 T1373 punct .,identified
R1164 T1402 T1410 amod SHARPIN-deficient,sensitized
R1165 T1403 T1410 nsubjpass mouse,sensitized
R1166 T1404 T1405 amod embryonic,fibroblast
R1167 T1405 T1403 appos fibroblast,mouse
R1168 T1406 T1407 punct (,MEF
R1169 T1407 T1405 appos MEF,fibroblast
R1170 T1408 T1403 punct ),mouse
R1171 T1409 T1410 auxpass were,sensitized
R1172 T1410 T1410 ROOT sensitized,sensitized
R1173 T1411 T1410 prep to,sensitized
R1174 T1412 T1413 advmod TNFα-induced,apoptosis
R1175 T1413 T1411 pobj apoptosis,to
R1176 T1414 T1413 cc and,apoptosis
R1177 T1415 T1416 compound cell,death
R1178 T1416 T1418 nsubjpass death,implicated
R1179 T1417 T1418 auxpass was,implicated
R1180 T1418 T1410 conj implicated,sensitized
R1181 T1419 T1418 prep as,implicated
R1182 T1420 T1421 det a,factor
R1183 T1421 T1419 pobj factor,as
R1184 T1422 T1421 prep in,factor
R1185 T1423 T1424 det the,dermatitis
R1186 T1424 T1422 pobj dermatitis,in
R1187 T1425 T1424 prep of,dermatitis
R1188 T1426 T1427 compound cpdm,mice
R1189 T1427 T1425 pobj mice,of
R1190 T1428 T1430 nmod [,]
R1191 T1429 T1430 nummod 7,]
R1192 T1430 T1431 nmod ],[
R1193 T1431 T1433 nmod [,]
R1194 T1432 T1433 nummod 9,]
R1195 T1433 T1418 conj ],implicated
R1196 T1434 T1418 punct .,implicated
R1197 T1435 T1436 amod Dendritic,cells
R1198 T1436 T1440 nsubj cells,have
R1199 T1437 T1438 punct (,DC
R1200 T1438 T1436 appos DC,cells
R1201 T1439 T1436 punct ),cells
R1202 T1440 T1440 ROOT have,have
R1203 T1441 T1443 det a,role
R1204 T1442 T1443 compound sentinel,role
R1205 T1443 T1440 dobj role,have
R1206 T1444 T1443 prep in,role
R1207 T1445 T1444 pcomp sensing,in
R1208 T1446 T1445 dobj pathogen,sensing
R1209 T1447 T1446 cc or,pathogen
R1210 T1448 T1446 conj danger,pathogen
R1211 T1449 T1445 dobj signals,sensing
R1212 T1450 T1445 cc and,sensing
R1213 T1451 T1445 conj initiate,sensing
R1214 T1452 T1451 cc and,initiate
R1215 T1453 T1454 amod direct,activation
R1216 T1454 T1451 conj activation,initiate
R1217 T1455 T1454 prep of,activation
R1218 T1456 T1459 det the,response
R1219 T1457 T1459 amod adaptive,response
R1220 T1458 T1459 amod immune,response
R1221 T1459 T1455 pobj response,of
R1222 T1460 T1462 nmod [,]
R1223 T1461 T1462 nummod 10,]
R1224 T1462 T1454 nmod ],activation
R1225 T1463 T1440 punct .,have
R1226 T1464 T1465 amod regulatory,molecule
R1227 T1465 T1114 attr molecule,is
R1228 T1466 T1465 prep in,molecule
R1229 T1467 T1468 compound DC,biology
R1230 T1468 T1466 pobj biology,in
R1231 T1469 T1114 cc and,is
R1232 T1470 T1114 conj suggest,is
R1233 T1471 T1478 mark that,plays
R1234 T1472 T1474 det the,function
R1235 T1473 T1474 amod dysregulated,function
R1236 T1474 T1478 nsubj function,plays
R1237 T1475 T1474 prep of,function
R1238 T1476 T1477 compound SHARPIN-deficient,DC
R1239 T1477 T1475 pobj DC,of
R1240 T1478 T1470 ccomp plays,suggest
R1241 T1479 T1480 det a,role
R1242 T1480 T1478 dobj role,plays
R1243 T1481 T1480 prep in,role
R1244 T1482 T1484 det the,phenotype
R1245 T1483 T1484 compound cpdm,phenotype
R1246 T1484 T1481 pobj phenotype,in
R1247 T1485 T1111 punct .,reveal
R1806 T2226 T2234 nsubjpass Characterization,found
R1807 T2227 T2226 prep of,Characterization
R1808 T2228 T2229 compound Sharpin,Orthologs
R1809 T2229 T2227 pobj Orthologs,of
R1810 T2230 T2229 prep of,Orthologs
R1811 T2231 T2232 compound SHARPIN,protein
R1812 T2232 T2230 pobj protein,of
R1813 T2233 T2234 auxpass are,found
R1814 T2234 T2234 ROOT found,found
R1815 T2235 T2234 prep in,found
R1816 T2236 T2237 amod various,species
R1817 T2237 T2235 pobj species,in
R1818 T2238 T2237 punct ",",species
R1819 T2239 T2237 prep including,species
R1820 T2240 T2239 pobj human,including
R1821 T2241 T2240 punct ",",human
R1822 T2242 T2240 conj mouse,human
R1823 T2243 T2242 cc and,mouse
R1824 T2244 T2242 conj rat,mouse
R1825 T2245 T2234 punct .,found
R1826 T2246 T2248 compound Motif,programming
R1827 T2247 T2248 compound prediction,programming
R1828 T2248 T2261 nsubj programming,suggests
R1829 T2249 T2248 punct ",",programming
R1830 T2250 T2248 acl using,programming
R1831 T2251 T2252 compound COILS,[
R1832 T2252 T2250 dobj [,using
R1833 T2253 T2254 nummod 11,]
R1834 T2254 T2252 conj ],[
R1835 T2255 T2254 cc and,]
R1836 T2256 T2257 compound MotifScan,[
R1837 T2257 T2254 conj [,]
R1838 T2258 T2259 nummod 12,]
R1839 T2259 T2254 conj ],]
R1840 T2260 T2261 punct ",",suggests
R1841 T2261 T2261 ROOT suggests,suggests
R1842 T2262 T2264 mark that,contains
R1843 T2263 T2264 nsubj SHARPIN,contains
R1844 T2264 T2261 ccomp contains,suggests
R1845 T2265 T2266 det a,coiled-coil
R1846 T2266 T2264 dobj coiled-coil,contains
R1847 T2267 T2268 punct (,CC
R1848 T2268 T2266 appos CC,coiled-coil
R1849 T2269 T2268 punct ),CC
R1850 T2270 T2266 conj domain,coiled-coil
R1851 T2271 T2266 punct ",",coiled-coil
R1852 T2272 T2273 det a,ubiquitin-like
R1853 T2273 T2266 appos ubiquitin-like,coiled-coil
R1854 T2274 T2275 punct (,UBL
R1855 T2275 T2273 appos UBL,ubiquitin-like
R1856 T2276 T2275 punct ),UBL
R1857 T2277 T2273 conj domain,ubiquitin-like
R1858 T2278 T2264 punct ",",contains
R1859 T2279 T2264 cc and,contains
R1860 T2280 T2283 det a,protein
R1861 T2281 T2283 nmod zinc-finger,protein
R1862 T2282 T2283 compound Ran-binding,protein
R1863 T2283 T2288 nmod protein,domain
R1864 T2284 T2283 nummod 2,protein
R1865 T2285 T2283 punct (,protein
R1866 T2286 T2283 appos ZFRBP,protein
R1867 T2287 T2283 punct ),protein
R1868 T2288 T2264 conj domain,contains
R1869 T2289 T2261 punct .,suggests
R1870 T2290 T2292 det These,motifs
R1871 T2291 T2292 amod functional,motifs
R1872 T2292 T2293 nsubj motifs,constitute
R1873 T2293 T2293 ROOT constitute,constitute
R1874 T2294 T2296 amod similar,profiles
R1875 T2295 T2296 compound domain,profiles
R1876 T2296 T2293 dobj profiles,constitute
R1877 T2297 T2298 nsubj that,are
R1878 T2298 T2296 relcl are,profiles
R1879 T2299 T2298 acomp present,are
R1880 T2300 T2299 prep in,present
R1881 T2301 T2303 det the,protein
R1882 T2302 T2303 compound SHARPIN,protein
R1883 T2303 T2300 pobj protein,in
R1884 T2304 T2303 prep of,protein
R1885 T2305 T2307 det all,origins
R1886 T2306 T2307 nummod three,origins
R1887 T2307 T2304 pobj origins,of
R1888 T2308 T2307 punct (,origins
R1889 T2309 T2310 compound Fig.,1A
R1890 T2310 T2307 appos 1A,origins
R1891 T2311 T2307 punct ),origins
R1892 T2312 T2296 punct ",",profiles
R1893 T2313 T2296 acl suggesting,profiles
R1894 T2314 T2318 mark that,conserved
R1895 T2315 T2318 nsubj SHARPIN,conserved
R1896 T2316 T2318 aux exerts,conserved
R1897 T2317 T2318 advmod highly,conserved
R1898 T2318 T2313 ccomp conserved,suggesting
R1899 T2319 T2318 dobj functions,conserved
R1900 T2320 T2319 prep across,functions
R1901 T2321 T2320 pobj species,across
R1902 T2322 T2293 punct .,constitute
R1903 T2323 T2324 amod Spontaneous,mutations
R1904 T2324 T2324 ROOT mutations,mutations
R1905 T2325 T2324 prep in,mutations
R1906 T2326 T2330 det the,results
R1907 T2327 T2329 compound mouse,gene
R1908 T2328 T2329 compound Sharpin,gene
R1909 T2329 T2330 compound gene,results
R1910 T2330 T2325 pobj results,in
R1911 T2331 T2324 prep in,mutations
R1912 T2332 T2335 det a,phenotype
R1913 T2333 T2335 amod complex,phenotype
R1914 T2334 T2335 amod inflammatory,phenotype
R1915 T2335 T2331 pobj phenotype,in
R1916 T2336 T2335 acl characterized,phenotype
R1917 T2337 T2336 agent by,characterized
R1918 T2338 T2339 amod severe,dermatitis
R1919 T2339 T2337 pobj dermatitis,by
R1920 T2340 T2342 punct (,1B
R1921 T2341 T2342 compound Fig.,1B
R1922 T2342 T2339 appos 1B,dermatitis
R1923 T2343 T2339 punct ),dermatitis
R1924 T2344 T2339 punct ",",dermatitis
R1925 T2345 T2346 amod systemic,inflammation
R1926 T2346 T2339 conj inflammation,dermatitis
R1927 T2347 T2346 cc and,inflammation
R1928 T2348 T2350 det an,spleen
R1929 T2349 T2350 amod enlarged,spleen
R1930 T2350 T2346 conj spleen,inflammation
R1931 T2351 T2350 punct (,spleen
R1932 T2352 T2353 compound Fig.,1C
R1933 T2353 T2350 appos 1C,spleen
R1934 T2354 T2350 punct ),spleen
R1935 T2355 T2346 acl caused,inflammation
R1936 T2356 T2355 agent by,caused
R1937 T2357 T2359 amod extramedullary,[
R1938 T2358 T2359 compound hematopoiesis,[
R1939 T2359 T2361 nmod [,]
R1940 T2360 T2359 nummod 3,[
R1941 T2361 T2356 pobj ],by
R1942 T2362 T2324 punct .,mutations
R1943 T2363 T2365 det The,expression
R1944 T2364 T2365 amod endogenous,expression
R1945 T2365 T2372 nsubjpass expression,determined
R1946 T2366 T2365 prep of,expression
R1947 T2367 T2368 compound Sharpin,mRNA
R1948 T2368 T2366 pobj mRNA,of
R1949 T2369 T2368 prep in,mRNA
R1950 T2370 T2369 pobj BMDC,in
R1951 T2371 T2372 auxpass was,determined
R1952 T2372 T2372 ROOT determined,determined
R1953 T2373 T2372 agent by,determined
R1954 T2374 T2376 amod quantitative,time-PCR
R1955 T2375 T2376 amod real,time-PCR
R1956 T2376 T2373 pobj time-PCR,by
R1957 T2377 T2376 punct (,time-PCR
R1958 T2378 T2376 appos qRT-PCR,time-PCR
R1959 T2379 T2376 punct ),time-PCR
R1960 T2380 T2372 prep following,determined
R1961 T2381 T2380 pobj culture,following
R1962 T2382 T2380 prep in,following
R1963 T2383 T2382 pobj medium,in
R1964 T2384 T2388 advmod only,with
R1965 T2385 T2384 cc or,only
R1966 T2386 T2384 conj after,only
R1967 T2387 T2386 pobj stimulation,after
R1968 T2388 T2380 prep with,following
R1969 T2389 T2388 pobj LPS,with
R1970 T2390 T2380 punct .,following
R1971 T2391 T2392 compound Sharpin,mRNA
R1972 T2392 T2393 nsubj mRNA,was
R1973 T2393 T2393 ROOT was,was
R1974 T2394 T2393 acomp present,was
R1975 T2395 T2394 prep in,present
R1976 T2396 T2395 pobj BMDC,in
R1977 T2397 T2393 conj generated,was
R1978 T2398 T2397 prep from,generated
R1979 T2399 T2400 compound WT,mice
R1980 T2400 T2398 pobj mice,from
R1981 T2401 T2403 punct (,1D
R1982 T2402 T2403 compound Fig.,1D
R1983 T2403 T2400 appos 1D,mice
R1984 T2404 T2403 punct ),1D
R1985 T2405 T2400 cc and,mice
R1986 T2406 T2407 poss its,level
R1987 T2407 T2410 nsubjpass level,decreased
R1988 T2408 T2410 auxpass was,decreased
R1989 T2409 T2410 advmod modestly,decreased
R1990 T2410 T2393 conj decreased,was
R1991 T2411 T2410 agent by,decreased
R1992 T2412 T2413 compound LPS,stimulation
R1993 T2413 T2411 pobj stimulation,by
R1994 T2414 T2410 punct .,decreased
R1995 T2415 T2416 expl There,was
R1996 T2416 T2416 ROOT was,was
R1997 T2417 T2419 det a,reduction
R1998 T2418 T2419 amod significant,reduction
R1999 T2419 T2416 attr reduction,was
R2000 T2420 T2419 prep of,reduction
R2001 T2421 T2422 compound Sharpin,mRNA
R2002 T2422 T2420 pobj mRNA,of
R2003 T2423 T2422 punct (,mRNA
R2004 T2424 T2422 appos 6,mRNA
R2005 T2425 T2422 appos 7-fold,mRNA
R2006 T2426 T2425 punct ),7-fold
R2007 T2427 T2425 prep in,7-fold
R2008 T2428 T2427 pobj BMDC,in
R2009 T2429 T2419 acl generated,reduction
R2010 T2430 T2429 prep from,generated
R2011 T2431 T2432 amod cpdm,mice
R2012 T2432 T2430 pobj mice,from
R2013 T2433 T2416 punct .,was
R2014 T2434 T2449 nsubj Transfection,indicated
R2015 T2435 T2434 prep of,Transfection
R2016 T2436 T2437 compound Flag-tagged,Sharpin
R2017 T2437 T2435 pobj Sharpin,of
R2018 T2438 T2434 prep in,Transfection
R2019 T2439 T2438 pobj fibroblasts,in
R2020 T2440 T2439 punct (,fibroblasts
R2021 T2441 T2439 appos NIH3T3,fibroblasts
R2022 T2442 T2439 punct ),fibroblasts
R2023 T2443 T2434 cc and,Transfection
R2024 T2444 T2434 conj macrophages,Transfection
R2025 T2445 T2444 punct (,macrophages
R2026 T2446 T2447 nummod RAW264,.7
R2027 T2447 T2444 appos .7,macrophages
R2028 T2448 T2444 punct ),macrophages
R2029 T2449 T2449 ROOT indicated,indicated
R2030 T2450 T2451 amod cytoplasmic,localization
R2031 T2451 T2449 dobj localization,indicated
R2032 T2452 T2451 prep of,localization
R2033 T2453 T2455 det the,protein
R2034 T2454 T2455 compound SHARPIN,protein
R2035 T2455 T2452 pobj protein,of
R2036 T2456 T2455 punct (,protein
R2037 T2457 T2458 compound Fig.,1E
R2038 T2458 T2455 appos 1E,protein
R2039 T2459 T2449 punct ),indicated
R2040 T2460 T2449 punct .,indicated
R2330 T2885 T2885 ROOT Phenotyping,Phenotyping
R2331 T2886 T2887 compound splenic,DC
R2332 T2887 T2894 nmod DC,mice
R2333 T2888 T2887 cc and,DC
R2334 T2889 T2887 conj BMDC,DC
R2335 T2890 T2889 prep from,BMDC
R2336 T2891 T2890 pobj WT,from
R2337 T2892 T2891 cc and,WT
R2338 T2893 T2894 compound cpdm,mice
R2339 T2894 T2885 dobj mice,Phenotyping
R2340 T2895 T2896 nsubj DC,are
R2341 T2896 T2885 ccomp are,Phenotyping
R2342 T2897 T2896 acomp heterogeneous,are
R2343 T2898 T2896 cc and,are
R2344 T2899 T2901 aux can,categorized
R2345 T2900 T2901 auxpass be,categorized
R2346 T2901 T2896 conj categorized,are
R2347 T2902 T2901 prep into,categorized
R2348 T2903 T2904 amod multiple,subtypes
R2349 T2904 T2902 pobj subtypes,into
R2350 T2905 T2904 acl based,subtypes
R2351 T2906 T2905 prep on,based
R2352 T2907 T2908 compound surface,markers
R2353 T2908 T2906 pobj markers,on
R2354 T2909 T2911 nmod [,]
R2355 T2910 T2911 nummod 13,]
R2356 T2911 T2908 appos ],markers
R2357 T2912 T2885 punct .,Phenotyping
R2358 T2913 T2914 aux To,determine
R2359 T2914 T2929 advcl determine,examined
R2360 T2915 T2919 mark if,affects
R2361 T2916 T2918 det the,mutation
R2362 T2917 T2918 compound Sharpin,mutation
R2363 T2918 T2919 nsubj mutation,affects
R2364 T2919 T2914 ccomp affects,determine
R2365 T2920 T2921 compound DC,development
R2366 T2921 T2919 dobj development,affects
R2367 T2922 T2921 prep in,development
R2368 T2923 T2924 amod lymphoid,tissues
R2369 T2924 T2922 pobj tissues,in
R2370 T2925 T2929 punct ",",examined
R2371 T2926 T2927 compound mouse,spleens
R2372 T2927 T2929 nsubjpass spleens,examined
R2373 T2928 T2929 auxpass were,examined
R2374 T2929 T2929 ROOT examined,examined
R2375 T2930 T2929 prep for,examined
R2376 T2931 T2932 det the,distribution
R2377 T2932 T2930 pobj distribution,for
R2378 T2933 T2932 prep of,distribution
R2379 T2934 T2935 amod conventional,DC
R2380 T2935 T2933 pobj DC,of
R2381 T2936 T2937 punct (,cDC
R2382 T2937 T2932 appos cDC,distribution
R2383 T2938 T2937 punct ;,cDC
R2384 T2939 T2942 compound CD11c,+
R2385 T2940 T2942 compound +,+
R2386 T2941 T2942 compound CD8α,+
R2387 T2942 T2966 dative +,.
R2388 T2943 T2942 cc and,+
R2389 T2944 T2942 conj CD11c,+
R2390 T2945 T2947 compound +,−
R2391 T2946 T2947 compound CD8α,−
R2392 T2947 T2942 appos −,+
R2393 T2948 T2947 punct ),−
R2394 T2949 T2951 nmod [,]
R2395 T2950 T2951 nummod 13,]
R2396 T2951 T2942 conj ],+
R2397 T2952 T2951 cc and,]
R2398 T2953 T2954 amod plasmacytoid,DC
R2399 T2954 T2951 conj DC,]
R2400 T2955 T2956 punct (,pDC
R2401 T2956 T2951 conj pDC,]
R2402 T2957 T2956 punct ;,pDC
R2403 T2958 T2961 compound CD11c,+
R2404 T2959 T2961 compound −,+
R2405 T2960 T2961 compound PDCA-1,+
R2406 T2961 T2966 dative +,.
R2407 T2962 T2963 punct ),[
R2408 T2963 T2965 nmod [,]
R2409 T2964 T2965 nummod 14,]
R2410 T2965 T2961 dobj ],+
R2411 T2966 T2929 punct .,examined
R2412 T2967 T2968 det The,percentages
R2413 T2968 T2976 nsubjpass percentages,reduced
R2414 T2969 T2968 prep for,percentages
R2415 T2970 T2971 compound splenic,cDC
R2416 T2971 T2969 pobj cDC,for
R2417 T2972 T2971 cc and,cDC
R2418 T2973 T2971 conj pDC,cDC
R2419 T2974 T2976 auxpass were,reduced
R2420 T2975 T2976 dep both,reduced
R2421 T2976 T2976 ROOT reduced,reduced
R2422 T2977 T2976 prep in,reduced
R2423 T2978 T2979 compound cpdm,mice
R2424 T2979 T2977 pobj mice,in
R2425 T2980 T2981 advmod when,compared
R2426 T2981 T2976 advcl compared,reduced
R2427 T2982 T2981 prep with,compared
R2428 T2983 T2984 compound WT,controls
R2429 T2984 T2982 pobj controls,with
R2430 T2985 T2987 punct (,2A
R2431 T2986 T2987 compound Fig.,2A
R2432 T2987 T2984 appos 2A,controls
R2433 T2988 T2984 punct ),controls
R2434 T2989 T2976 punct .,reduced
R2435 T2990 T3010 advmod However,affected
R2436 T2991 T3010 punct ",",affected
R2437 T2992 T2993 advmod when,gated
R2438 T2993 T3010 advcl gated,affected
R2439 T2994 T2993 prep on,gated
R2440 T2995 T2997 amod CD11c,cDC
R2441 T2996 T2997 compound +,cDC
R2442 T2997 T2994 pobj cDC,on
R2443 T2998 T2997 punct ",",cDC
R2444 T2999 T3000 det the,percentages
R2445 T3000 T3010 nsubjpass percentages,affected
R2446 T3001 T3000 prep of,percentages
R2447 T3002 T3003 amod CD8α,+
R2448 T3003 T3001 pobj +,of
R2449 T3004 T3003 cc and,+
R2450 T3005 T3007 amod CD8α,cells
R2451 T3006 T3007 compound −,cells
R2452 T3007 T3003 conj cells,+
R2453 T3008 T3010 auxpass were,affected
R2454 T3009 T3010 neg not,affected
R2455 T3010 T3010 ROOT affected,affected
R2456 T3011 T3010 agent by,affected
R2457 T3012 T3013 compound SHARPIN,deficiency
R2458 T3013 T3011 pobj deficiency,by
R2459 T3014 T3013 punct (,deficiency
R2460 T3015 T3016 compound Fig.,2A
R2461 T3016 T3013 appos 2A,deficiency
R2462 T3017 T3013 punct ),deficiency
R2463 T3018 T3010 punct .,affected
R2464 T3019 T3025 mark Since,is
R2465 T3020 T3021 det the,spleen
R2466 T3021 T3025 nsubj spleen,is
R2467 T3022 T3021 prep of,spleen
R2468 T3023 T3024 compound cpdm,mice
R2469 T3024 T3022 pobj mice,of
R2470 T3025 T3025 ROOT is,is
R2471 T3026 T3027 advmod markedly,enlarged
R2472 T3027 T3025 acomp enlarged,is
R2473 T3028 T3025 cc and,is
R2474 T3029 T3025 conj contains,is
R2475 T3030 T3034 nummod three,cells
R2476 T3031 T3030 quantmod times,three
R2477 T3032 T3033 advmod as,many
R2478 T3033 T3034 amod many,cells
R2479 T3034 T3029 dobj cells,contains
R2480 T3035 T3034 punct (,cells
R2481 T3036 T3037 compound Fig.,1C
R2482 T3037 T3034 appos 1C,cells
R2483 T3038 T3034 punct ),cells
R2484 T3039 T3034 punct ",",cells
R2485 T3040 T3042 det the,percentages
R2486 T3041 T3042 amod different,percentages
R2487 T3042 T3034 appos percentages,cells
R2488 T3043 T3042 prep of,percentages
R2489 T3044 T3045 compound splenic,cDC
R2490 T3045 T3043 pobj cDC,of
R2491 T3046 T3045 cc and,cDC
R2492 T3047 T3045 conj pDC,cDC
R2493 T3048 T3042 prep between,percentages
R2494 T3049 T3048 pobj WT,between
R2495 T3050 T3049 cc and,WT
R2496 T3051 T3052 amod mutant,mice
R2497 T3052 T3049 conj mice,WT
R2498 T3053 T3029 conj reflect,contains
R2499 T3054 T3056 det the,number
R2500 T3055 T3056 amod increased,number
R2501 T3056 T3053 dobj number,reflect
R2502 T3057 T3056 prep of,number
R2503 T3058 T3060 amod total,cells
R2504 T3059 T3060 compound spleen,cells
R2505 T3060 T3057 pobj cells,of
R2506 T3061 T3062 advmod rather,than
R2507 T3062 T3056 cc than,number
R2508 T3063 T3064 det a,reduction
R2509 T3064 T3056 conj reduction,number
R2510 T3065 T3064 prep in,reduction
R2511 T3066 T3065 pobj cDC,in
R2512 T3067 T3066 cc and,cDC
R2513 T3068 T3066 conj pDC,cDC
R2514 T3069 T3064 conj numbers,reduction
R2515 T3070 T3025 punct .,is
R2516 T3071 T3072 det These,data
R2517 T3072 T3073 nsubj data,indicate
R2518 T3073 T3073 ROOT indicate,indicate
R2519 T3074 T3080 mark that,affect
R2520 T3075 T3077 det the,mutation
R2521 T3076 T3077 compound Sharpin,mutation
R2522 T3077 T3080 nsubj mutation,affect
R2523 T3078 T3080 aux does,affect
R2524 T3079 T3080 neg not,affect
R2525 T3080 T3073 ccomp affect,indicate
R2526 T3081 T3082 det the,distribution
R2527 T3082 T3080 dobj distribution,affect
R2528 T3083 T3082 prep of,distribution
R2529 T3084 T3087 det the,subsets
R2530 T3085 T3087 amod examined,subsets
R2531 T3086 T3087 compound DC,subsets
R2532 T3087 T3083 pobj subsets,of
R2533 T3088 T3087 prep in,subsets
R2534 T3089 T3090 det the,spleen
R2535 T3090 T3088 pobj spleen,in
R2536 T3091 T3073 punct .,indicate
R2537 T3092 T3098 nsubj BMDC,resemble
R2538 T3093 T3092 prep from,BMDC
R2539 T3094 T3093 prep in,from
R2540 T3095 T3096 compound vitro,cultures
R2541 T3096 T3094 pobj cultures,in
R2542 T3097 T3098 advmod functionally,resemble
R2543 T3098 T3098 ROOT resemble,resemble
R2544 T3099 T3101 compound non-lymphoid,DC
R2545 T3100 T3101 compound tissue,DC
R2546 T3101 T3098 dobj DC,resemble
R2547 T3102 T3101 cc and,DC
R2548 T3103 T3108 amod monocyte-derived,]
R2549 T3104 T3108 nmod inflammatory,]
R2550 T3105 T3106 compound DC,[
R2551 T3106 T3104 appos [,inflammatory
R2552 T3107 T3108 nummod 15,]
R2553 T3108 T3101 conj ],DC
R2554 T3109 T3108 punct ",",]
R2555 T3110 T3112 nmod [,]
R2556 T3111 T3112 nummod 16,]
R2557 T3112 T3108 conj ],]
R2558 T3113 T3098 punct .,resemble
R2559 T3114 T3115 det The,yields
R2560 T3115 T3123 nsubj yields,were
R2561 T3116 T3115 prep of,yields
R2562 T3117 T3116 pobj BMDC,of
R2563 T3118 T3117 prep from,BMDC
R2564 T3119 T3118 pobj WT,from
R2565 T3120 T3117 cc and,BMDC
R2566 T3121 T3122 compound cpdm,mice
R2567 T3122 T3123 nsubj mice,were
R2568 T3123 T3123 ROOT were,were
R2569 T3124 T3123 acomp similar,were
R2570 T3125 T3123 punct .,were
R2571 T3126 T3127 nsubj BMDC,were
R2572 T3127 T3127 ROOT were,were
R2573 T3128 T3129 amod CD11c,+
R2574 T3129 T3127 attr +,were
R2575 T3130 T3129 cc and,+
R2576 T3131 T3133 compound MHC,+
R2577 T3132 T3133 compound II,+
R2578 T3133 T3129 conj +,+
R2579 T3134 T3129 prep with,+
R2580 T3135 T3136 amod low,expression
R2581 T3136 T3134 pobj expression,with
R2582 T3137 T3136 prep of,expression
R2583 T3138 T3139 amod co-stimulatory,molecules
R2584 T3139 T3137 pobj molecules,of
R2585 T3140 T3136 appos CD40,expression
R2586 T3141 T3140 punct ",",CD40
R2587 T3142 T3140 conj CD80,CD40
R2588 T3143 T3142 punct ",",CD80
R2589 T3144 T3142 cc and,CD80
R2590 T3145 T3142 conj CD86,CD80
R2591 T3146 T3127 punct .,were
R2592 T3147 T3150 det The,poly
R2593 T3148 T3150 nummod TLR3,poly
R2594 T3149 T3150 compound ligand,poly
R2595 T3150 T3160 nsubj poly,activate
R2596 T3151 T3153 nsubj I,C
R2597 T3152 T3153 punct :,C
R2598 T3153 T3150 appos C,poly
R2599 T3154 T3153 cc and,C
R2600 T3155 T3158 det the,LPS
R2601 T3156 T3158 nummod TLR4,LPS
R2602 T3157 T3158 compound ligand,LPS
R2603 T3158 T3153 conj LPS,C
R2604 T3159 T3160 dep each,activate
R2605 T3160 T3160 ROOT activate,activate
R2606 T3161 T3160 xcomp overlapping,activate
R2607 T3162 T3160 cc but,activate
R2608 T3163 T3165 amod different,pathways
R2609 T3164 T3165 compound signaling,pathways
R2610 T3165 T3160 dobj pathways,activate
R2611 T3166 T3160 cc and,activate
R2612 T3167 T3168 auxpass were,used
R2613 T3168 T3160 conj used,activate
R2614 T3169 T3170 aux to,induce
R2615 T3170 T3168 xcomp induce,used
R2616 T3171 T3177 nmod DC,[
R2617 T3172 T3175 nmod maturation,]
R2618 T3173 T3175 nmod [,]
R2619 T3174 T3175 nummod 17,]
R2620 T3175 T3177 nmod ],[
R2621 T3176 T3177 punct ",",[
R2622 T3177 T3179 nmod [,]
R2623 T3178 T3179 nummod 18,]
R2624 T3179 T3170 dobj ],induce
R2625 T3180 T3160 punct .,activate
R2626 T3181 T3189 nsubj Incubation,resulted
R2627 T3182 T3181 prep with,Incubation
R2628 T3183 T3185 det the,agonists
R2629 T3184 T3185 compound TLR,agonists
R2630 T3185 T3182 pobj agonists,with
R2631 T3186 T3181 prep for,Incubation
R2632 T3187 T3188 nummod 24,hours
R2633 T3188 T3186 pobj hours,for
R2634 T3189 T3206 ccomp resulted,was
R2650 T3205 T3206 expl there,was
R2651 T3206 T3206 ROOT was,was
R2652 T3207 T3208 det no,difference
R2653 T3208 T3206 attr difference,was
R2654 T3209 T3208 prep in,difference
R2655 T3210 T3212 det the,levels
R2656 T3211 T3212 compound expression,levels
R2657 T3212 T3209 pobj levels,in
R2658 T3213 T3212 prep of,levels
R2659 T3214 T3215 det these,markers
R2660 T3215 T3213 pobj markers,of
R2661 T3216 T3215 prep between,markers
R2662 T3217 T3216 pobj WT,between
R2663 T3218 T3217 cc and,WT
R2664 T3219 T3220 compound cpdm,BMDC
R2665 T3220 T3217 conj BMDC,WT
R2666 T3221 T3223 punct (,2B
R2667 T3222 T3223 compound Fig.,2B
R2668 T3223 T3220 appos 2B,BMDC
R2669 T3224 T3223 punct ),2B
R2670 T3225 T3206 punct .,was
R2671 T3226 T3232 advmod Thus,influence
R2672 T3227 T3232 punct ",",influence
R2673 T3228 T3229 compound SHARPIN,deficiency
R2674 T3229 T3232 nsubj deficiency,influence
R2675 T3230 T3232 aux did,influence
R2676 T3231 T3232 neg not,influence
R2677 T3232 T3232 ROOT influence,influence
R3135 T3828 T3836 nsubjpass Production,impaired
R3136 T3829 T3828 prep of,Production
R3137 T3830 T3831 amod proinflammatory,mediators
R3138 T3831 T3829 pobj mediators,of
R3139 T3832 T3828 prep by,Production
R3140 T3833 T3834 compound cpdm,BMDC
R3141 T3834 T3832 pobj BMDC,by
R3142 T3835 T3836 auxpass is,impaired
R3143 T3836 T3868 ccomp impaired,produced
R3144 T3837 T3836 dobj Incubation,impaired
R3145 T3838 T3836 prep with,impaired
R3146 T3839 T3838 pobj LPS,with
R3147 T3840 T3836 cc or,impaired
R3148 T3841 T3845 advmod poly,resulted
R3149 T3842 T3845 nsubj I,resulted
R3150 T3843 T3845 punct :,resulted
R3151 T3844 T3845 nsubj C,resulted
R3152 T3845 T3836 conj resulted,impaired
R3153 T3846 T3845 prep in,resulted
R3154 T3847 T3846 pobj secretion,in
R3155 T3848 T3847 prep of,secretion
R3156 T3849 T3848 pobj IL6,of
R3157 T3850 T3849 punct ",",IL6
R3158 T3851 T3849 conj IL12P70,IL6
R3159 T3852 T3851 punct ",",IL12P70
R3160 T3853 T3851 cc and,IL12P70
R3161 T3854 T3851 conj GMCSF,IL12P70
R3162 T3855 T3845 prep from,resulted
R3238 T3931 T3929 dobj phenotype,reversed
R3239 T3932 T3931 prep of,phenotype
R3240 T3933 T3935 det the,mice
R3241 T3934 T3935 amod mutant,mice
R3242 T3935 T3932 pobj mice,of
R3243 T3936 T3938 nmod [,]
R3244 T3937 T3938 nummod 2,]
R3245 T3938 T3947 nsubj ],secreted
R3246 T3939 T3938 punct ",",]
R3247 T3940 T3938 cc and,]
R3248 T3941 T3938 conj BMDC,]
R3249 T3942 T3938 acl generated,]
R3250 T3943 T3942 prep from,generated
R3251 T3944 T3946 det these,mice
R3252 T3945 T3946 amod rescued,mice
R3253 T3946 T3943 pobj mice,from
R3254 T3947 T3929 conj secreted,reversed
R3255 T3948 T3949 advmod significantly,more
R3256 T3949 T3950 advmod more,IL12P70
R3257 T3950 T3947 dobj IL12P70,secreted
R3258 T3951 T3950 prep than,IL12P70
R3259 T3952 T3951 pobj BMDC,than
R3260 T3953 T3952 prep from,BMDC
R3261 T3954 T3955 compound cpdm,mice
R3262 T3955 T3953 pobj mice,from
R3263 T3956 T3952 punct (,BMDC
R3264 T3957 T3958 compound Fig.,3G
R3265 T3958 T3952 appos 3G,BMDC
R3266 T3959 T3952 punct ),BMDC
R3267 T3960 T3947 punct ",",secreted
R3268 T3961 T3947 advcl supporting,secreted
R3269 T3962 T3964 det a,role
R3270 T3963 T3964 amod necessary,role
R3271 T3964 T3961 dobj role,supporting
R3272 T3965 T3964 prep of,role
R3273 T3966 T3965 pobj SHARPIN,of
R3274 T3967 T3964 prep for,role
R3275 T3968 T3969 det the,production
R3276 T3969 T3967 pobj production,for
R3277 T3970 T3969 prep of,production
R3278 T3971 T3970 pobj IL12P70,of
R3279 T3972 T3947 punct .,secreted
R3280 T3973 T3992 prep In,examined
R3281 T3974 T3973 pobj addition,In
R3282 T3975 T3992 punct ",",examined
R3283 T3976 T3978 det the,levels
R3284 T3977 T3978 compound transcript,levels
R3285 T3978 T3992 nsubjpass levels,examined
R3286 T3979 T3978 prep of,levels
R3287 T3980 T3983 det the,Il6
R3288 T3981 T3982 amod inflammatory,cytokines
R3289 T3982 T3983 compound cytokines,Il6
R3290 T3983 T3979 pobj Il6,of
R3291 T3984 T3983 punct ",",Il6
R3292 T3985 T3983 conj Il12p40,Il6
R3293 T3986 T3985 punct ",",Il12p40
R3294 T3987 T3985 conj Gmcsf,Il12p40
R3295 T3988 T3987 punct ",",Gmcsf
R3296 T3989 T3987 cc and,Gmcsf
R3297 T3990 T3987 conj Ifnb,Gmcsf
R3298 T3991 T3992 auxpass were,examined
R3299 T3992 T3992 ROOT examined,examined
R3300 T3993 T3992 punct ",",examined
R3301 T3994 T3992 cc and,examined
R3302 T3995 T3996 nsubj these,were
R3303 T3996 T3999 auxpass were,reduced
R3304 T3997 T3999 dep all,reduced
R3305 T3998 T3999 advmod significantly,reduced
R3306 T3999 T3992 conj reduced,examined
R3307 T4000 T3999 prep in,reduced
R3308 T4001 T4003 amod stimulated,BMDC
R3309 T4002 T4003 compound cpdm,BMDC
R3310 T4003 T4000 pobj BMDC,in
R3311 T4004 T4005 advmod when,compared
R3312 T4005 T3999 advcl compared,reduced
R3649 T4514 T4510 pobj secretion,for
R3650 T4515 T4514 prep in,secretion
R3651 T4516 T4518 amod stimulated,BMDC
R3652 T4517 T4518 compound cpdm,BMDC
R3653 T4518 T4515 pobj BMDC,in
R3654 T4519 T4514 punct ",",secretion
R3655 T4520 T4509 prep including,explanations
R3656 T4521 T4520 pobj 1,including
R3657 T4522 T4509 punct ),explanations
R3658 T4523 T4525 amod reduced,expression
R3659 T4524 T4525 compound surface,expression
R3660 T4525 T4506 conj expression,number
R3661 T4526 T4525 prep of,expression
R3662 T4527 T4530 det the,complex
R3663 T4528 T4530 compound LPS,complex
R3664 T4529 T4530 compound receptor,complex
R3665 T4530 T4526 pobj complex,of
R3666 T4531 T4525 punct ",",expression
R3667 T4532 T4525 appos 2,expression
R3668 T4533 T4525 punct ),expression
R3669 T4534 T4535 amod increased,production
R3670 T4535 T4535 ROOT production,production
R3671 T4536 T4535 prep of,production
R3672 T4537 T4538 amod anti-inflammatory,mediators
R3673 T4538 T4536 pobj mediators,of
R3674 T4539 T4535 punct ",",production
R3675 T4540 T4542 meta 3,increased
R3676 T4541 T4540 punct ),3
R3677 T4542 T4543 amod increased,expression
R3678 T4543 T4535 appos expression,production
R3679 T4544 T4543 prep of,expression
R3680 T4545 T4546 amod negative,regulators
R3681 T4546 T4544 pobj regulators,of
R3682 T4547 T4546 prep of,regulators
R3683 T4548 T4549 compound TLR,pathways
R3684 T4549 T4547 pobj pathways,of
R3685 T4550 T4535 punct ",",production
R3686 T4551 T4535 cc and,production
R3687 T4552 T4557 meta 4,activation
R3688 T4553 T4552 punct ),4
R3689 T4554 T4557 amod impaired,activation
R3690 T4555 T4557 amod TLR-induced,activation
R3691 T4556 T4557 compound signaling,activation
R3692 T4557 T4535 conj activation,production
R3693 T4558 T4496 punct .,correlated
R3694 T4559 T4560 nsubj We,determined
R3695 T4560 T4560 ROOT determined,determined
R3696 T4561 T4563 det the,expression
R3697 T4562 T4563 compound surface,expression
R3698 T4563 T4560 dobj expression,determined
R3699 T4564 T4563 prep of,expression
R3700 T4565 T4568 det the,complex
R3701 T4566 T4568 compound LPS,complex
R3702 T4567 T4568 compound receptor,complex
R3703 T4568 T4564 pobj complex,of
R3704 T4569 T4570 nsubj that,comprises
R3705 T4570 T4568 relcl comprises,complex
R3706 T4571 T4570 dobj TLR4,comprises
R2678 T3233 T3234 det the,expression
R2679 T3234 T3232 dobj expression,influence
R2680 T3235 T3234 prep of,expression
R2681 T3236 T3237 amod co-stimulatory,molecules
R2682 T3237 T3235 pobj molecules,of
R2683 T3238 T3232 prep by,influence
R2684 T3239 T3238 pobj BMDC,by
R2685 T3240 T3232 punct .,influence
R3163 T3856 T3857 preconj both,WT
R3164 T3857 T3855 pobj WT,from
R3165 T3858 T3857 cc and,WT
R3166 T3859 T3860 compound cpdm,BMDC
R3167 T3860 T3857 conj BMDC,WT
R3168 T3861 T3868 punct ;,produced
R3169 T3862 T3868 advmod however,produced
R3170 T3863 T3868 punct ",",produced
R3171 T3864 T3868 nsubj BMDC,produced
R3172 T3865 T3864 prep from,BMDC
R3173 T3866 T3867 compound cpdm,mice
R3174 T3867 T3865 pobj mice,from
R3175 T3868 T3868 ROOT produced,produced
R3176 T3869 T3870 advmod significantly,less
R3177 T3870 T3868 dobj less,produced
R3178 T3871 T3870 prep of,less
R3179 T3872 T3874 det all,cytokines
R3180 T3873 T3874 nummod three,cytokines
R3181 T3874 T3871 pobj cytokines,of
R3182 T3875 T3870 prep compared,less
R3183 T3876 T3875 prep with,compared
R3184 T3877 T3878 compound WT,BMDC
R3185 T3878 T3876 pobj BMDC,with
R3186 T3879 T3878 punct (,BMDC
R3187 T3880 T3881 compound Fig.,3A
R3188 T3881 T3878 appos 3A,BMDC
R3189 T3882 T3878 conj C,BMDC
R3190 T3883 T3882 punct ",",C
R3191 T3884 T3885 compound E,F
R3192 T3885 T3882 appos F,C
R3193 T3886 T3882 punct ),C
R3194 T3887 T3868 punct .,produced
R3195 T3888 T3889 det The,amount
R3196 T3889 T3897 nsubj amount,was
R3197 T3890 T3889 prep of,amount
R3198 T3891 T3892 amod nitric,oxide
R3199 T3892 T3890 pobj oxide,of
R3200 T3893 T3892 acl generated,oxide
R3201 T3894 T3893 agent by,generated
R3202 T3895 T3896 amod mutant,BMDC
R3203 T3896 T3894 pobj BMDC,by
R3204 T3897 T3897 ROOT was,was
R3205 T3898 T3897 advmod also,was
R3206 T3899 T3900 advmod significantly,reduced
R3207 T3900 T3897 acomp reduced,was
R3208 T3901 T3900 prep compared,reduced
R3209 T3902 T3901 prep with,compared
R3210 T3903 T3904 compound WT,cells
R3211 T3904 T3902 pobj cells,with
R3212 T3905 T3907 punct (,3D
R3213 T3906 T3907 compound Fig.,3D
R3214 T3907 T3904 appos 3D,cells
R3215 T3908 T3900 punct ),reduced
R3216 T3909 T3897 punct ",",was
R3217 T3910 T3897 advcl indicating,was
R3218 T3911 T3912 advmod severely,disrupted
R3219 T3912 T3913 amod disrupted,production
R3220 T3913 T3910 dobj production,indicating
R3221 T3914 T3913 prep of,production
R3222 T3915 T3916 amod proinflammatory,mediators
R3223 T3916 T3914 pobj mediators,of
R3224 T3917 T3913 prep from,production
R3225 T3918 T3919 compound Sharpin-deficient,BMDC
R3226 T3919 T3917 pobj BMDC,from
R3227 T3920 T3897 punct .,was
R3228 T3921 T3929 prep In,reversed
R3229 T3922 T3923 compound vivo,complementation
R3230 T3923 T3921 pobj complementation,In
R3231 T3924 T3923 prep with,complementation
R3232 T3925 T3928 det a,BAC
R3233 T3926 T3928 compound Sharpin,BAC
R3234 T3927 T3928 compound gene-containing,BAC
R3235 T3928 T3924 pobj BAC,with
R3236 T3929 T3929 ROOT reversed,reversed
R3237 T3930 T3931 det the,phenotype
R3313 T4006 T4005 prep with,compared
R3314 T4007 T4008 compound WT,controls
R3315 T4008 T4006 pobj controls,with
R3316 T4009 T4010 punct (,Fig.
R3317 T4010 T4008 parataxis Fig.,controls
R3318 T4011 T4010 nummod 4,Fig.
R3319 T4012 T4010 punct ),Fig.
R3320 T4013 T3992 punct .,examined
R3624 T4489 T4491 amod Impaired,production
R3625 T4490 T4491 amod cytokine,production
R3626 T4491 T4496 nsubjpass production,correlated
R3627 T4492 T4491 prep by,production
R3628 T4493 T4494 compound cpdm,BMDC
R3629 T4494 T4492 pobj BMDC,by
R3630 T4495 T4496 auxpass is,correlated
R3631 T4496 T4496 ROOT correlated,correlated
R3632 T4497 T4496 prep with,correlated
R3633 T4498 T4499 amod selective,defects
R3634 T4499 T4497 pobj defects,with
R3635 T4500 T4499 prep in,defects
R3636 T4501 T4502 amod NF-κB,signaling
R3637 T4502 T4496 advcl signaling,correlated
R3638 T4503 T4504 expl There,are
R3639 T4504 T4502 ccomp are,signaling
R3640 T4505 T4506 det a,number
R3641 T4506 T4504 attr number,are
R3642 T4507 T4506 prep of,number
R3643 T4508 T4509 amod possible,explanations
R3644 T4509 T4507 pobj explanations,of
R3645 T4510 T4509 prep for,explanations
R3646 T4511 T4514 det the,secretion
R3647 T4512 T4514 amod defective,secretion
R3648 T4513 T4514 compound cytokine,secretion
R3724 T4589 T4592 nsubj Flow,shows
R3725 T4590 T4591 amod cytometric,analysis
R3726 T4591 T4592 nsubj analysis,shows
R3727 T4592 T4592 ROOT shows,shows
R3728 T4593 T4606 mark that,were
R3729 T4594 T4596 det the,levels
R3730 T4595 T4596 compound expression,levels
R3731 T4596 T4606 nsubj levels,were
R3732 T4597 T4596 prep of,levels
R3733 T4598 T4597 pobj CD14,of
R3734 T4599 T4598 cc and,CD14
R3735 T4600 T4598 conj TLR4/MD2,CD14
R3736 T4601 T4596 prep between,levels
R3737 T4602 T4601 pobj WT,between
R3738 T4603 T4602 cc and,WT
R3739 T4604 T4605 compound cpdm,BMDC
R3740 T4605 T4602 conj BMDC,WT
R3741 T4606 T4592 ccomp were,shows
R3742 T4607 T4606 acomp similar,were
R3743 T4608 T4610 punct (,5A
R3744 T4609 T4610 compound Fig.,5A
R3745 T4610 T4606 parataxis 5A,were
R3746 T4611 T4610 punct ),5A
R3747 T4612 T4592 punct .,shows
R3748 T4613 T4615 nsubj We,quantified
R3749 T4614 T4615 advmod then,quantified
R3750 T4615 T4615 ROOT quantified,quantified
R3751 T4616 T4617 det the,secretion
R3752 T4617 T4615 dobj secretion,quantified
R3753 T4618 T4617 prep of,secretion
R3754 T4619 T4621 det the,cytokines
R3755 T4620 T4621 amod suppressive,cytokines
R3756 T4621 T4618 pobj cytokines,of
R3757 T4622 T4615 dobj IL10,quantified
R3758 T4623 T4625 nsubj that,inhibit
R3759 T4624 T4625 aux can,inhibit
R3760 T4625 T4615 ccomp inhibit,quantified
R3761 T4626 T4627 compound IL12,secretion
R3762 T4627 T4625 dobj secretion,inhibit
R3763 T4628 T4625 prep in,inhibit
R3764 T4629 T4631 det an,manner
R3765 T4630 T4631 compound autocrine,manner
R3707 T4572 T4571 punct ",",TLR4
R3708 T4573 T4575 det the,proteins
R3709 T4574 T4575 amod accessory,proteins
R3710 T4575 T4571 conj proteins,TLR4
R3711 T4576 T4580 nmod CD14,factor
R3712 T4577 T4576 cc and,CD14
R3713 T4578 T4580 amod myeloid,factor
R3714 T4579 T4580 compound differentiation,factor
R3715 T4580 T4575 dobj factor,proteins
R3716 T4581 T4580 nummod 2,factor
R3717 T4582 T4585 punct (,[
R3718 T4583 T4585 nmod MD2/LY96,[
R3719 T4584 T4585 punct ),[
R3720 T4585 T4580 appos [,factor
R3721 T4586 T4587 nummod 19,]
R3722 T4587 T4585 appos ],[
R3723 T4588 T4560 punct .,determined
R3799 T4664 T4661 ccomp was,suggesting
R3800 T4665 T4664 neg not,was
R3801 T4666 T4664 acomp responsible,was
R3802 T4667 T4666 prep for,responsible
R3803 T4668 T4669 amod decreased,secretion
R3804 T4669 T4667 pobj secretion,for
R3805 T4670 T4669 prep of,secretion
R3806 T4671 T4670 pobj IL12P70,of
R3807 T4672 T4669 prep by,secretion
R3808 T4673 T4674 compound cpdm,BMDC
R3809 T4674 T4672 pobj BMDC,by
R3810 T4675 T4646 punct .,contained
R3811 T4676 T4677 amod Increased,expression
R3812 T4677 T4693 nsubj expression,suppress
R3813 T4678 T4677 prep of,expression
R3814 T4679 T4681 det a,regulator
R3815 T4680 T4681 amod negative,regulator
R3816 T4681 T4678 pobj regulator,of
R3817 T4682 T4681 prep of,regulator
R3818 T4683 T4682 pobj TLR,of
R3819 T4684 T4677 acl signaling,expression
R3820 T4685 T4686 amod such,as
R3821 T4686 T4684 prep as,signaling
R3822 T4687 T4688 compound A20,[
R3823 T4688 T4686 pobj [,as
R3824 T4689 T4690 nummod 22,]
R3825 T4690 T4693 nsubj ],suppress
R3826 T4691 T4693 aux may,suppress
R3827 T4692 T4693 advmod also,suppress
R3828 T4693 T4693 ROOT suppress,suppress
R3829 T4694 T4695 amod cytokine,secretion
R3830 T4695 T4693 dobj secretion,suppress
R3831 T4696 T4693 punct .,suppress
R3832 T4697 T4704 advmod However,was
R3833 T4698 T4704 punct ",",was
R3834 T4699 T4701 det the,level
R3835 T4700 T4701 compound transcript,level
R3836 T4701 T4704 nsubj level,was
R3837 T4702 T4701 prep of,level
R3838 T4703 T4702 pobj A20,of
R3839 T4704 T4704 ROOT was,was
R3840 T4705 T4704 acomp lower,was
R3841 T4706 T4705 prep in,lower
R3842 T4707 T4709 amod LPS-activated,BMDC
R3843 T4708 T4709 compound cpdm,BMDC
R3844 T4709 T4706 pobj BMDC,in
R3845 T4710 T4709 prep than,BMDC
R3846 T4711 T4712 amod WT,controls
R3847 T4712 T4710 pobj controls,than
R3848 T4713 T4715 punct (,5C
R3849 T4714 T4715 compound Fig.,5C
R3850 T4715 T4712 appos 5C,controls
R3851 T4716 T4709 punct ),BMDC
R3852 T4717 T4704 punct ",",was
R3853 T4718 T4719 advmod thereby,ruling
R3854 T4719 T4704 advcl ruling,was
R3855 T4720 T4719 prt out,ruling
R3856 T4721 T4719 dobj overexpression,ruling
R3857 T4722 T4721 prep of,overexpression
R3858 T4723 T4722 pobj A20,of
R3859 T4724 T4719 prep as,ruling
R3860 T4725 T4726 det a,factor
R3861 T4726 T4724 pobj factor,as
R3862 T4727 T4726 prep in,factor
R3863 T4728 T4731 det the,production
R3864 T4729 T4731 amod reduced,production
R3865 T4730 T4731 compound cytokine,production
R3866 T4731 T4727 pobj production,in
R3867 T4732 T4704 punct .,was
R3868 T4733 T4734 det The,transcription
R3869 T4734 T4741 nsubjpass transcription,regulated
R3870 T4735 T4734 prep of,transcription
R3871 T4736 T4738 amod TLR3/4-induced,intermediates
R3872 T4737 T4738 amod proinflammatory,intermediates
R3948 T4813 T4802 ccomp release,allowing
R3949 T4814 T4817 det the,factors
R3950 T4815 T4817 amod NF-κB,factors
R3951 T4816 T4817 compound transcription,factors
R3952 T4817 T4813 dobj factors,release
R3953 T4818 T4813 prep into,release
R3954 T4819 T4820 det the,nucleus
R3955 T4820 T4818 pobj nucleus,into
R3956 T4821 T4794 punct .,is
R3957 T4822 T4823 det The,amount
R3958 T4823 T4848 nsubjpass amount,decreased
R3959 T4824 T4823 prep of,amount
R3960 T4825 T4826 amod phosphorylated,IKK1/2
R3961 T4826 T4824 pobj IKK1/2,of
R3962 T4827 T4826 punct (,IKK1/2
R3963 T4828 T4829 amod p-IKK1,/
R3964 T4829 T4826 appos /,IKK1/2
R3965 T4830 T4829 nummod 2,/
R3966 T4831 T4829 punct ),/
R3967 T4832 T4826 cc and,IKK1/2
R3968 T4833 T4826 conj IκBα,IKK1/2
R3969 T4834 T4835 punct (,p-IκBα
R3970 T4835 T4833 appos p-IκBα,IκBα
R3971 T4836 T4823 punct ),amount
R3972 T4837 T4823 acl following,amount
R3973 T4838 T4837 pobj incubation,following
R3974 T4839 T4838 prep with,incubation
R3975 T4840 T4839 pobj LPS,with
R3976 T4841 T4837 cc or,following
R3977 T4842 T4848 advmod poly,decreased
R3978 T4843 T4848 nsubjpass I,decreased
R3979 T4844 T4848 punct :,decreased
R3980 T4845 T4848 nsubjpass C,decreased
R3981 T4846 T4848 auxpass was,decreased
R3982 T4847 T4848 advmod severely,decreased
R3983 T4848 T4848 ROOT decreased,decreased
R3984 T4849 T4848 prep in,decreased
R3985 T4850 T4851 compound cpdm,BMDC
R3986 T4851 T4849 pobj BMDC,in
R3987 T4852 T4848 prep as,decreased
R3988 T4853 T4852 prep compared,as
R3989 T4854 T4853 prep with,compared
R3990 T4855 T4856 compound WT,controls
R3991 T4856 T4854 pobj controls,with
R3992 T4857 T4858 punct (,Fig.
R3993 T4858 T4856 appos Fig.,controls
R3994 T4859 T4858 nummod 6,Fig.
R3995 T4860 T4858 punct ),Fig.
R3996 T4861 T4848 punct .,decreased
R3997 T4862 T4864 det The,BMDC
R3998 T4863 T4864 compound cpdm,BMDC
R3999 T4864 T4865 nsubj BMDC,exhibited
R4000 T4865 T4865 ROOT exhibited,exhibited
R4001 T4866 T4867 amod similar,levels
R4002 T4867 T4865 dobj levels,exhibited
R4003 T4868 T4867 prep of,levels
R3766 T4631 T4628 pobj manner,in
R3767 T4632 T4634 nmod [,]
R3768 T4633 T4634 nummod 20,]
R3769 T4634 T4625 npadvmod ],inhibit
R3770 T4635 T4634 punct ",",]
R3771 T4636 T4638 nmod [,]
R3772 T4637 T4638 nummod 21,]
R3773 T4638 T4634 appos ],]
R3774 T4639 T4615 punct .,quantified
R3775 T4640 T4641 det The,supernatants
R3776 T4641 T4646 nsubj supernatants,contained
R3777 T4642 T4641 prep from,supernatants
R3778 T4643 T4644 amod LPS-stimulated,cpdm
R3779 T4644 T4645 compound cpdm,BMDC
R3780 T4645 T4642 pobj BMDC,from
R3781 T4646 T4646 ROOT contained,contained
R3782 T4647 T4648 advmod significantly,lower
R3783 T4648 T4649 amod lower,levels
R3784 T4649 T4646 dobj levels,contained
R3785 T4650 T4649 prep of,levels
R3786 T4651 T4650 pobj IL10,of
R3787 T4652 T4653 mark than,stimulated
R3788 T4653 T4655 amod stimulated,BMDC
R3789 T4654 T4655 compound WT,BMDC
R3790 T4655 T4649 appos BMDC,levels
R3791 T4656 T4658 punct (,5B
R3792 T4657 T4658 compound Fig.,5B
R3793 T4658 T4655 appos 5B,BMDC
R3794 T4659 T4658 punct ),5B
R3795 T4660 T4646 punct ",",contained
R3796 T4661 T4646 advcl suggesting,contained
R3797 T4662 T4664 mark that,was
R3798 T4663 T4664 nsubj IL10,was
R3873 T4738 T4735 pobj intermediates,of
R3874 T4739 T4741 auxpass is,regulated
R3875 T4740 T4741 advmod tightly,regulated
R3876 T4741 T4741 ROOT regulated,regulated
R3877 T4742 T4741 agent by,regulated
R3878 T4743 T4745 amod cellular,pathways
R3879 T4744 T4745 amod signaling,pathways
R3880 T4745 T4742 pobj pathways,by
R3881 T4746 T4745 punct ",",pathways
R3882 T4747 T4757 prep in,]
R3883 T4748 T4747 amod particular,in
R3884 T4749 T4757 nmod NF-κB,]
R3885 T4750 T4749 punct ",",NF-κB
R3886 T4751 T4749 appos TBK1/IRF3,NF-κB
R3887 T4752 T4749 punct ",",NF-κB
R3888 T4753 T4749 cc and,NF-κB
R3889 T4754 T4755 compound MAPK,[
R3890 T4755 T4749 conj [,NF-κB
R3891 T4756 T4757 nummod 23,]
R3892 T4757 T4745 appos ],pathways
R3893 T4758 T4760 nmod [,]
R3894 T4759 T4760 nummod 27,]
R3895 T4760 T4757 appos ],]
R3896 T4761 T4741 punct .,regulated
R3897 T4762 T4764 nsubjpass We,determined
R3898 T4763 T4764 advmod next,determined
R3899 T4764 T4764 ROOT determined,determined
R3900 T4765 T4766 mark if,disrupted
R3901 T4766 T4764 advcl disrupted,determined
R3902 T4767 T4766 dobj NF-κB,disrupted
R3903 T4768 T4767 punct ",",NF-κB
R3904 T4769 T4767 appos TBK1/IRF3,NF-κB
R3905 T4770 T4766 punct ",",disrupted
R3906 T4771 T4766 cc and/or,disrupted
R3907 T4772 T4773 compound MAPK,signaling
R3908 T4773 T4775 nsubj signaling,underlie
R3909 T4774 T4775 aux may,underlie
R3910 T4775 T4764 ccomp underlie,determined
R3911 T4776 T4779 det the,production
R3912 T4777 T4779 amod impaired,production
R3913 T4778 T4779 compound cytokine,production
R3914 T4779 T4775 dobj production,underlie
R3915 T4780 T4779 prep from,production
R3916 T4781 T4783 amod stimulated,BMDC
R3917 T4782 T4783 compound Sharpin-deficient,BMDC
R3918 T4783 T4780 pobj BMDC,from
R3919 T4784 T4775 punct .,underlie
R3920 T4785 T4786 amod Stimulus-induced,phosphorylation
R3921 T4786 T4794 nsubj phosphorylation,is
R3922 T4787 T4786 prep of,phosphorylation
R3923 T4788 T4790 det the,kinase
R3924 T4789 T4790 compound IκB,kinase
R3925 T4790 T4787 pobj kinase,of
R3926 T4791 T4792 punct (,IKK1/2
R3927 T4792 T4790 appos IKK1/2,kinase
R3928 T4793 T4790 punct ),kinase
R3929 T4794 T4794 ROOT is,is
R3930 T4795 T4797 det an,step
R3931 T4796 T4797 amod essential,step
R3932 T4797 T4794 attr step,is
R3933 T4798 T4797 prep in,step
R3934 T4799 T4800 amod NF-κB,signaling
R3935 T4800 T4798 pcomp signaling,in
R3936 T4801 T4800 punct ",",signaling
R3937 T4802 T4800 conj allowing,signaling
R3938 T4803 T4806 amod phosphorylation,degradation
R3939 T4804 T4803 cc and,phosphorylation
R3940 T4805 T4803 conj proteasome-mediated,phosphorylation
R3941 T4806 T4802 dobj degradation,allowing
R3942 T4807 T4806 prep of,degradation
R3943 T4808 T4810 det the,inhibitor
R3944 T4809 T4810 compound NF-κB,inhibitor
R3945 T4810 T4807 pobj inhibitor,of
R3946 T4811 T4806 appos IκBα,degradation
R3947 T4812 T4813 aux to,release
R4023 T4888 T4888 ROOT indicate,indicate
R4024 T4889 T4895 mark that,decreased
R4025 T4890 T4891 det the,absence
R4026 T4891 T4895 nsubj absence,decreased
R4027 T4892 T4891 prep of,absence
R4028 T4893 T4894 amod functional,SHARPIN
R4029 T4894 T4892 pobj SHARPIN,of
R4030 T4895 T4888 ccomp decreased,indicate
R4031 T4896 T4897 compound NF-κB,activation
R4032 T4897 T4895 dobj activation,decreased
R4033 T4898 T4895 cc but,decreased
R4034 T4899 T4901 aux did,affect
R4035 T4900 T4901 neg not,affect
R4036 T4901 T4895 conj affect,decreased
R4037 T4902 T4901 dobj TBK1/IRF3,affect
R4038 T4903 T4902 punct ",",TBK1/IRF3
R4039 T4904 T4902 conj ERK1/2,TBK1/IRF3
R4040 T4905 T4904 punct ",",ERK1/2
R4041 T4906 T4904 cc and,ERK1/2
R4042 T4907 T4908 nummod p38,signaling
R4043 T4908 T4904 conj signaling,ERK1/2
R4044 T4909 T4908 prep in,signaling
R4045 T4910 T4909 pobj BMDC,in
R4046 T4911 T4888 punct .,indicate
R4651 T5830 T5831 amod Th2-biased,immunogenicity
R4652 T5831 T5854 nsubj immunogenicity,suggest
R4653 T5832 T5831 prep of,immunogenicity
R4654 T5833 T5834 amod stimulated,cpdm
R4655 T5834 T5832 pobj cpdm,of
R4656 T5835 T5839 nmod BMDC,production
R4657 T5836 T5839 det The,production
R4658 T5837 T5839 amod defective,production
R4659 T5838 T5839 compound IL12,production
R4660 T5839 T5847 nmod production,profile
R4661 T5840 T5839 punct (,production
R4662 T5841 T5842 compound Fig.,3A
R4663 T5842 T5839 appos 3A,production
R4664 T5843 T5839 punct ),production
R4665 T5844 T5839 cc and,production
R4666 T5845 T5847 amod Th2-dominant,profile
R4667 T5846 T5847 compound cytokine,profile
R4668 T5847 T5831 conj profile,immunogenicity
R4669 T5848 T5847 prep in,profile
R4670 T5849 T5850 compound cpdm,mice
R4671 T5850 T5848 pobj mice,in
R4672 T5851 T5854 amod [,suggest
R4673 T5852 T5853 nummod 5,]
R4674 T5853 T5854 nsubj ],suggest
R4675 T5854 T5854 ROOT suggest,suggest
R4676 T5855 T5860 mark that,affects
R4004 T4869 T4868 pobj TBK1,of
R4005 T4870 T4865 punct ",",exhibited
R4006 T4871 T4865 npadvmod ERK1/2,exhibited
R4007 T4872 T4871 punct ",",ERK1/2
R4008 T4873 T4871 cc and,ERK1/2
R4009 T4874 T4875 nummod p38,phosphorylation
R4010 T4875 T4865 npadvmod phosphorylation,exhibited
R4011 T4876 T4875 prep to,phosphorylation
R4012 T4877 T4876 pobj those,to
R4013 T4878 T4877 prep of,those
R4014 T4879 T4880 compound WT,cells
R4015 T4880 T4878 pobj cells,of
R4016 T4881 T4882 punct (,Fig.
R4017 T4882 T4877 appos Fig.,those
R4018 T4883 T4882 nummod 6,Fig.
R4019 T4884 T4882 punct ),Fig.
R4020 T4885 T4865 punct .,exhibited
R4021 T4886 T4887 det These,results
R4022 T4887 T4888 nsubj results,indicate
R4727 T5906 T5908 compound BMDC-T,cultures
R4728 T5907 T5908 compound cell,cultures
R4729 T5908 T5904 pobj cultures,in
R4730 T5909 T5894 advcl was,elicited
R4731 T5910 T5911 advmod significantly,lower
R4732 T5911 T5909 acomp lower,was
R4733 T5912 T5909 prep after,was
R4734 T5913 T5914 compound LPS,stimulation
R4735 T5914 T5912 pobj stimulation,after
R4736 T5915 T5914 punct (,stimulation
R4737 T5916 T5917 compound Fig.,7A
R4738 T5917 T5914 appos 7A,stimulation
R4739 T5918 T5914 punct ),stimulation
R4740 T5919 T5909 punct ",",was
R4741 T5920 T5909 advcl indicating,was
R4742 T5921 T5923 amod impaired,abilities
R4743 T5922 T5923 amod Th1-polarizing,abilities
R4744 T5923 T5920 dobj abilities,indicating
R4745 T5924 T5923 prep of,abilities
R4746 T5925 T5926 compound cpdm,BMDC
R4747 T5926 T5924 pobj BMDC,of
R4748 T5927 T5894 punct .,elicited
R4749 T5928 T5939 prep In,used
R4750 T5929 T5928 pobj addition,In
R4751 T5930 T5929 prep to,addition
R4752 T5931 T5932 nummod TLR3/4,agonists
R4753 T5932 T5930 pobj agonists,to
R4754 T5933 T5932 punct ",",agonists
R4755 T5934 T5937 det the,Pam3CYS
R4756 T5935 T5937 nummod TLR2,Pam3CYS
R4757 T5936 T5937 compound ligand,Pam3CYS
R4758 T5937 T5939 nsubjpass Pam3CYS,used
R4759 T5938 T5939 auxpass was,used
R4760 T5939 T5939 ROOT used,used
R4761 T5940 T5944 mark since,shown
R4762 T5941 T5944 nsubjpass it,shown
R4763 T5942 T5944 aux has,shown
R4764 T5943 T5944 auxpass been,shown
R4765 T5944 T5939 advcl shown,used
R4766 T5945 T5946 aux to,induce
R4767 T5946 T5944 xcomp induce,shown
R4768 T5947 T5948 det both,Th1
R4769 T5948 T5946 dobj Th1,induce
R4770 T5949 T5948 cc and,Th1
R4771 T5950 T5948 conj Th2,Th1
R4772 T5951 T5939 nsubjpass responses,used
R4773 T5952 T5954 compound [,]
R4774 T5953 T5954 compound 28,]
R4775 T5954 T5951 nsubj ],responses
R4776 T5955 T5957 nmod [,]
R4777 T5956 T5957 nummod 30,]
R4778 T5957 T5954 appos ],]
R4779 T5958 T5939 punct .,used
R4780 T5959 T5961 compound Pam3CYS-matured,BMDC
R4781 T5960 T5961 compound WT,BMDC
R4782 T5961 T5962 nsubj BMDC,induced
R4783 T5962 T5962 ROOT induced,induced
R4784 T5963 T5965 amod robust,production
R4785 T5964 T5965 compound IFNγ,production
R4786 T5965 T5962 dobj production,induced
R4787 T5966 T5962 prep at,induced
R4788 T5967 T5970 det a,level
R4789 T5968 T5969 advmod significantly,higher
R4790 T5969 T5970 amod higher,level
R4791 T5970 T5966 pobj level,at
R4792 T5971 T5962 prep than,induced
R4793 T5972 T5973 compound cpdm,BMDC
R4794 T5973 T5971 pobj BMDC,than
R4795 T5974 T5976 punct (,7A
R4796 T5975 T5976 compound Fig.,7A
R4797 T5976 T5973 appos 7A,BMDC
R4798 T5977 T5976 punct ),7A
R4799 T5978 T5962 punct .,induced
R4800 T5979 T5982 det The,differentiation
R4801 T5980 T5982 amod reduced,differentiation
R4877 T6056 T6052 conj cocultures,WT
R4878 T6057 T6056 punct (,cocultures
R4879 T6058 T6061 nmod data,)
R4880 T6059 T6060 neg not,shown
R4881 T6060 T6058 acl shown,data
R4882 T6061 T6056 punct ),cocultures
R4883 T6062 T6048 punct .,was
R4884 T6063 T6078 prep Despite,were
R4885 T6064 T6070 det the,abilities
R4886 T6065 T6070 amod distinct,abilities
R4887 T6066 T6070 punct Th1,abilities
R4888 T6067 T6066 punct -,Th1
R4889 T6068 T6066 cc and,Th1
R4890 T6069 T6070 amod Th2-stimulating,abilities
R4891 T6070 T6063 pobj abilities,Despite
R4892 T6071 T6070 prep of,abilities
R4893 T6072 T6071 pobj WT,of
R4894 T6073 T6070 cc and,abilities
R4895 T6074 T6075 compound cpdm,BMDC
R4896 T6075 T6070 conj BMDC,abilities
R4897 T6076 T6078 punct ",",were
R4898 T6077 T6078 nsubj they,were
R4899 T6078 T6078 ROOT were,were
R4900 T6079 T6080 advmod equally,effective
R4901 T6080 T6078 acomp effective,were
R4902 T6081 T6080 prep in,effective
R4903 T6082 T6083 amod IL2,production
R4905 T6084 T6083 prep from,production
R4906 T6085 T6087 compound BMDC-T,co-cultures
R4907 T6086 T6087 compound cell,co-cultures
R4908 T6087 T6084 pobj co-cultures,from
R4909 T6088 T6083 prep except,production
R4910 T6089 T6088 prep for,except
R4911 T6090 T6089 pobj poly,for
R4912 T6091 T6078 nsubj I,were
R4913 T6092 T6091 punct :,I
R4914 T6093 T6094 compound C,stimulation
R4915 T6094 T6091 appos stimulation,I
R4916 T6095 T6098 advmod where,induced
R4917 T6096 T6097 compound WT,BMDC
R4918 T6097 T6098 nsubj BMDC,induced
R4919 T6098 T6094 relcl induced,stimulation
R4920 T6099 T6100 advmod more,IL2
R4921 T6100 T6098 dobj IL2,induced
R4922 T6101 T6100 prep than,IL2
R4923 T6102 T6103 compound cpdm,BMDC
R4924 T6103 T6101 pobj BMDC,than
R4925 T6104 T6103 punct (,BMDC
R4926 T6105 T6106 compound Fig.,7C
R4927 T6106 T6103 appos 7C,BMDC
R4677 T5856 T5857 det the,absence
R4678 T5857 T5860 nsubj absence,affects
R4679 T5858 T5857 prep of,absence
R4680 T5859 T5858 pobj SHARPIN,of
R4681 T5860 T5854 ccomp affects,suggest
R4682 T5861 T5862 det the,ability
R4683 T5862 T5860 dobj ability,affects
R4684 T5863 T5862 prep of,ability
R4685 T5864 T5865 compound cpdm,BMDC
R4686 T5865 T5863 pobj BMDC,of
R4687 T5866 T5867 aux to,induce
R4688 T5867 T5862 acl induce,ability
R4689 T5868 T5870 compound T,differentiation
R4690 T5869 T5870 compound cell,differentiation
R4691 T5870 T5867 dobj differentiation,induce
R4692 T5871 T5867 prep into,induce
R4693 T5872 T5873 amod effector,cells
R4694 T5873 T5871 pobj cells,into
R4695 T5874 T5854 punct .,suggest
R4696 T5875 T5886 nsubj Co-culture,stimulated
R4697 T5876 T5875 prep of,Co-culture
R4698 T5877 T5878 amod allogeneic,naïve
R4699 T5878 T5882 compound naïve,cells
R4700 T5879 T5882 compound CD4,cells
R4701 T5880 T5882 compound +,cells
R4702 T5881 T5882 compound T,cells
R4703 T5882 T5876 pobj cells,of
R4704 T5883 T5875 prep with,Co-culture
R4705 T5884 T5885 compound WT,BMDC
R4706 T5885 T5883 pobj BMDC,with
R4707 T5886 T5886 ROOT stimulated,stimulated
R4708 T5887 T5886 prep with,stimulated
R4709 T5888 T5887 pobj LPS,with
R4710 T5889 T5886 cc or,stimulated
R4711 T5890 T5894 advmod poly,elicited
R4712 T5891 T5894 nsubj I,elicited
R4713 T5892 T5894 punct :,elicited
R4714 T5893 T5894 nsubj C,elicited
R4715 T5894 T5886 conj elicited,stimulated
R4716 T5895 T5897 amod robust,production
R4717 T5896 T5897 compound IFNγ,production
R4718 T5897 T5894 dobj production,elicited
R4719 T5898 T5894 punct ",",elicited
R4720 T5899 T5909 mark whereas,was
R4721 T5900 T5901 det the,concentration
R4722 T5901 T5909 nsubj concentration,was
R4723 T5902 T5901 prep of,concentration
R4724 T5903 T5902 pobj IFNγ,of
R4725 T5904 T5901 prep in,concentration
R4726 T5905 T5906 compound cpdm,BMDC-T
R4802 T5981 T5982 compound Th1,differentiation
R4803 T5982 T5986 nsubj differentiation,is
R4804 T5983 T5982 prep following,differentiation
R4805 T5984 T5985 compound Pam3CYS,stimulation
R4806 T5985 T5983 pobj stimulation,following
R4807 T5986 T5986 ROOT is,is
R4808 T5987 T5986 acomp consistent,is
R4809 T5988 T5987 prep with,consistent
R4810 T5989 T5991 det the,report
R4811 T5990 T5991 amod recent,report
R4812 T5991 T5988 pobj report,with
R4813 T5992 T5991 prep of,report
R4814 T5993 T5995 amod decreased,production
R4815 T5994 T5995 compound IL12,production
R4816 T5995 T5992 pobj production,of
R4817 T5996 T5995 prep in,production
R4818 T5997 T5998 compound cpdm,macrophages
R4819 T5998 T5996 pobj macrophages,in
R4820 T5999 T5991 prep following,report
R4821 T6000 T6001 compound TLR2,stimulation
R4822 T6001 T5999 pobj stimulation,following
R4823 T6002 T6004 nmod [,]
R4824 T6003 T6004 nummod 31,]
R4825 T6004 T6001 appos ],stimulation
R4826 T6005 T5986 punct .,is
R4827 T6006 T6014 prep In,produced
R4828 T6007 T6006 pobj contrast,In
R4829 T6008 T6014 punct ",",produced
R4830 T6009 T6012 amod more,cytokine
R4831 T6010 T6012 amod Th2-specific,cytokine
R4832 T6011 T6012 compound IL4,cytokine
R4833 T6012 T6014 nsubjpass cytokine,produced
R4834 T6013 T6014 auxpass was,produced
R4835 T6014 T6014 ROOT produced,produced
R4836 T6015 T6014 prep in,produced
R4837 T6016 T6019 det the,co-cultures
R4838 T6017 T6019 compound cpdm,co-cultures
R4839 T6018 T6019 compound BMDC,co-cultures
R4840 T6019 T6015 pobj co-cultures,in
R4841 T6020 T6014 prep than,produced
R4842 T6021 T6023 det the,control
R4843 T6022 T6023 compound WT,control
R4844 T6023 T6020 pobj control,than
R4845 T6024 T6023 punct (,control
R4846 T6025 T6026 compound Fig.,7B
R4847 T6026 T6023 appos 7B,control
R4848 T6027 T6023 punct ),control
R4849 T6028 T6014 punct ",",produced
R4850 T6029 T6014 advcl suggesting,produced
R4851 T6030 T6031 amod Th2-skewed,immunogenicity
R4852 T6031 T6029 dobj immunogenicity,suggesting
R4853 T6032 T6031 prep of,immunogenicity
R4854 T6033 T6034 compound cpdm,BMDC
R4855 T6034 T6032 pobj BMDC,of
R4856 T6035 T6014 punct .,produced
R4857 T6036 T6037 det The,production
R4858 T6037 T6048 nsubj production,was
R4859 T6038 T6037 prep of,production
R4860 T6039 T6041 amod Th17-specific,IL17A
R4861 T6040 T6041 compound cytokine,IL17A
R4862 T6041 T6038 pobj IL17A,of
R4863 T6042 T6037 prep following,production
R4864 T6043 T6044 compound LPS,stimulation
R4865 T6044 T6042 pobj stimulation,following
R4866 T6045 T6044 prep of,stimulation
R4867 T6046 T6047 amod dendritic,cells
R4868 T6047 T6045 pobj cells,of
R4869 T6048 T6048 ROOT was,was
R4870 T6049 T6048 acomp similar,was
R4871 T6050 T6049 prep between,similar
R4872 T6051 T6052 amod stimulated,WT
R4873 T6052 T6050 pobj WT,between
R4874 T6053 T6052 cc and,WT
R4875 T6054 T6055 compound cpdm,BMDC
R4876 T6055 T6052 conj BMDC,WT
R4953 T6132 T6125 advcl observed,is
R4954 T6133 T6132 prep in,observed
R4955 T6134 T6135 compound cpdm,mice
R4956 T6135 T6133 pobj mice,in
R4957 T6136 T6125 punct .,is
R5411 T6805 T6807 det The,report
R5412 T6806 T6807 amod present,report
R5413 T6807 T6808 nsubj report,showed
R5414 T6808 T6808 ROOT showed,showed
R5415 T6809 T6819 mark that,affect
R5416 T6810 T6811 amod null,mutations
R5417 T6811 T6819 nsubj mutations,affect
R5418 T6812 T6811 prep of,mutations
R5419 T6813 T6816 det the,gene
R5420 T6814 T6816 compound mouse,gene
R5421 T6815 T6816 compound Sharpin,gene
R5422 T6816 T6812 pobj gene,of
R5423 T6817 T6819 aux did,affect
R5424 T6818 T6819 neg not,affect
R5425 T6819 T6808 ccomp affect,showed
R5426 T6820 T6822 det the,distribution
R5427 T6821 T6822 amod steady-state,distribution
R5428 T6822 T6819 dobj distribution,affect
R5429 T6823 T6822 prep of,distribution
R5430 T6824 T6826 amod splenic,subsets
R5431 T6825 T6826 compound DC,subsets
R5432 T6826 T6823 pobj subsets,of
R5433 T6827 T6822 cc nor,distribution
R5434 T6828 T6829 det the,development
R5435 T6829 T6822 conj development,distribution
R5436 T6830 T6829 cc and,development
R5437 T6831 T6829 conj phenotype,development
R5438 T6832 T6829 prep of,development
R4928 T6107 T6103 punct ),BMDC
R4929 T6108 T6078 punct .,were
R4930 T6109 T6112 det The,capability
R4931 T6110 T6112 amod Th2-biased,capability
R4932 T6111 T6112 amod stimulating,capability
R4933 T6112 T6125 nsubj capability,is
R4934 T6113 T6112 prep of,capability
R4935 T6114 T6115 compound cpdm,BMDC
R4936 T6115 T6113 pobj BMDC,of
R4937 T6116 T6117 advmod when,co-cultured
R4938 T6117 T6125 nsubj co-cultured,is
R4939 T6118 T6117 prep with,co-cultured
R4940 T6119 T6120 amod allogeneic,naïve
R4941 T6120 T6124 compound naïve,cells
R4942 T6121 T6124 compound CD4,cells
R4943 T6122 T6124 compound +,cells
R4944 T6123 T6124 compound T,cells
R4945 T6124 T6118 pobj cells,with
R4946 T6125 T6125 ROOT is,is
R4947 T6126 T6125 acomp consistent,is
R4948 T6127 T6126 prep with,consistent
R4949 T6128 T6131 det the,phenotype
R4950 T6129 T6131 amod Th2-dominant,phenotype
R4951 T6130 T6131 compound cytokine,phenotype
R4952 T6131 T6127 pobj phenotype,with
R5505 T6899 T6903 advmod Together,indicate
R5506 T6900 T6903 punct ",",indicate
R5507 T6901 T6902 det these,results
R5508 T6902 T6903 nsubj results,indicate
R5509 T6903 T6903 ROOT indicate,indicate
R5510 T6904 T6906 det an,role
R5511 T6905 T6906 amod indispensible,role
R5512 T6906 T6903 dobj role,indicate
R5513 T6907 T6906 prep of,role
R5514 T6908 T6907 pobj SHARPIN,of
R5515 T6909 T6906 prep in,role
R5516 T6910 T6909 pcomp regulating,in
R5517 T6911 T6913 nmod DC,functions
R5518 T6912 T6913 amod immunological,functions
R5519 T6913 T6910 dobj functions,regulating
R5520 T6914 T6915 punct ",",disruption
R5521 T6915 T6919 nsubj disruption,contribute
R5522 T6916 T6915 prep of,disruption
R5523 T6917 T6916 pobj which,of
R5524 T6918 T6919 aux may,contribute
R5525 T6919 T6903 ccomp contribute,indicate
R5526 T6920 T6919 prep to,contribute
R5527 T6921 T6922 det the,development
R5528 T6922 T6920 pobj development,to
R5529 T6923 T6922 prep of,development
R5530 T6924 T6925 amod immune,diseases
R5531 T6925 T6923 pobj diseases,of
R5532 T6926 T6903 punct .,indicate
R5533 T6927 T6932 mark Since,are
R5534 T6928 T6931 nmod WT,mice
R5535 T6929 T6928 cc and,WT
R5536 T6930 T6931 compound cpdm,mice
R5537 T6931 T6932 nsubj mice,are
R5538 T6932 T6949 csubj are,is
R5539 T6933 T6932 dep both,are
R5540 T6934 T6935 amod specific,pathogen-free
R5541 T6935 T6932 attr pathogen-free,are
R5542 T6936 T6935 punct ",",pathogen-free
R5543 T6937 T6938 det the,nature
R5544 T6938 T6935 appos nature,pathogen-free
R5545 T6939 T6938 prep of,nature
R5546 T6940 T6941 det the,trigger
R5547 T6941 T6939 pobj trigger,of
R5548 T6942 T6941 prep of,trigger
R5549 T6943 T6945 det the,inflammation
R5550 T6944 T6945 amod severe,inflammation
R5551 T6945 T6942 pobj inflammation,of
R5552 T6946 T6945 prep in,inflammation
R5553 T6947 T6948 compound cpdm,mice
R5554 T6948 T6946 pobj mice,in
R5555 T6949 T6949 ROOT is,is
R5556 T6950 T6949 neg not,is
R5557 T6951 T6949 acomp obvious,is
R5558 T6952 T6949 punct .,is
R5559 T6953 T6956 nummod One,factor
R5560 T6954 T6956 amod such,factor
R5561 T6955 T6956 amod initiating,factor
R5562 T6956 T6958 nsubj factor,be
R5563 T6957 T6958 aux could,be
R5564 T6958 T6958 ROOT be,be
R5565 T6959 T6960 advmod endogenous,apoptotic
R5566 T6960 T6963 amod apoptotic,cells
R5567 T6961 T6960 cc and/or,apoptotic
R5568 T6962 T6960 conj necrotic,apoptotic
R5569 T6963 T6958 attr cells,be
R5570 T6964 T6966 nsubj that,release
R5571 T6965 T6966 aux can,release
R5572 T6966 T6963 relcl release,cells
R5573 T6967 T6969 amod danger-associated,patterns
R5574 T6968 T6969 amod molecular,patterns
R5575 T6969 T6966 dobj patterns,release
R5576 T6970 T6969 punct (,patterns
R5577 T6971 T6969 appos DAMPs,patterns
R5578 T6972 T6969 punct ),patterns
R5579 T6973 T6974 aux to,launch
R5655 T7049 T7036 advcl suggesting,rescued
R5656 T7050 T7054 mark that,compromises
R5657 T7051 T7054 nsubj deficiency,compromises
R5658 T7052 T7051 prep of,deficiency
R5659 T7053 T7052 pobj SHARPIN,of
R5660 T7054 T7049 ccomp compromises,suggesting
R5661 T7055 T7057 det the,mechanisms
R5662 T7056 T7057 amod anti-apoptotic,mechanisms
R5663 T7057 T7054 dobj mechanisms,compromises
R5664 T7058 T7054 prep in,compromises
R5665 T7059 T7060 compound cpdm,mice
R5666 T7060 T7058 pobj mice,in
R5667 T7061 T7060 acl resulting,mice
R5668 T7062 T7061 prep in,resulting
R5669 T7063 T7066 nmod cell,disease
R5670 T7064 T7066 amod death-induced,disease
R5671 T7065 T7066 amod inflammatory,disease
R5672 T7066 T7062 pobj disease,in
R5673 T7067 T7036 punct .,rescued
R5674 T7068 T7071 nsubj Apoptosis,is
R5675 T7069 T7068 prep of,Apoptosis
R5676 T7070 T7069 pobj keratinocytes,of
R5695 T7089 T7088 agent by,mediated
R5696 T7090 T7092 amod caspase-dependent,pathways
R5697 T7091 T7092 amod mitochondrial,pathways
R5698 T7092 T7089 pobj pathways,by
R5699 T7093 T7095 nmod [,]
R5700 T7094 T7095 nummod 34,]
R5701 T7095 T7092 appos ],pathways
R5702 T7096 T7071 punct .,is
R5703 T7097 T7098 nsubj These,induced
R5704 T7098 T7104 advmod induced,release
R5705 T7099 T7098 cc and/or,induced
R5706 T7100 T7098 conj intrinsic,induced
R5707 T7101 T7102 amod apoptotic,cells
R5708 T7102 T7104 nsubj cells,release
R5709 T7103 T7104 aux can,release
R5710 T7104 T7104 ROOT release,release
R5711 T7105 T7106 amod various,types
R5712 T7106 T7104 dobj types,release
R5713 T7107 T7106 prep of,types
R5714 T7108 T7107 pobj DAMPs,of
R5439 T6833 T6832 pobj BMDC,of
R5440 T6834 T6808 punct .,showed
R5441 T6835 T6841 advmod However,diminished
R5442 T6836 T6841 punct ",",diminished
R5443 T6837 T6841 nsubj loss,diminished
R5444 T6838 T6837 prep of,loss
R5445 T6839 T6838 pobj SHARPIN,of
R5446 T6840 T6841 advmod significantly,diminished
R5459 T6853 T6841 punct .,diminished
R5460 T6854 T6857 det The,production
R5461 T6855 T6857 amod attenuated,production
R5462 T6856 T6857 compound cytokine,production
R5463 T6857 T6858 nsubj production,was
R5464 T6858 T6858 ROOT was,was
R5465 T6859 T6858 neg not,was
R5466 T6860 T6858 acomp due,was
R5467 T6861 T6860 prep to,due
R5468 T6862 T6863 det the,presence
R5469 T6863 T6861 pobj presence,to
R5470 T6864 T6863 prep of,presence
R5471 T6865 T6866 amod anti-inflammatory,inhibitors
R5472 T6866 T6864 pobj inhibitors,of
R5473 T6867 T6858 punct ",",was
R5474 T6868 T6858 cc and,was
R5475 T6869 T6872 aux can,explained
R5476 T6870 T6872 auxpass be,explained
R5477 T6871 T6872 advmod largely,explained
R5478 T6872 T6858 conj explained,was
R5479 T6873 T6872 agent by,explained
R5480 T6874 T6875 amod selective,inactivation
R5481 T6875 T6873 pobj inactivation,by
R5482 T6876 T6875 prep of,inactivation
R5483 T6877 T6878 amod NF-κB,signaling
R5484 T6878 T6876 pobj signaling,of
R5485 T6879 T6872 punct .,explained
R5486 T6880 T6883 advcl Stimulated,exhibited
R5487 T6881 T6882 compound cpdm,BMDC
R5488 T6882 T6883 nsubj BMDC,exhibited
R5489 T6883 T6883 ROOT exhibited,exhibited
R5490 T6884 T6887 amod Th2-biased,capabilities
R5491 T6885 T6887 compound T,capabilities
R5492 T6886 T6887 compound cell-polarizing,capabilities
R5493 T6887 T6883 dobj capabilities,exhibited
R5494 T6888 T6887 punct ",",capabilities
R5495 T6889 T6887 amod consistent,capabilities
R5496 T6890 T6889 prep with,consistent
R5497 T6891 T6894 det the,phenotype
R5498 T6892 T6894 nmod Th2,phenotype
R5499 T6893 T6894 amod cytokine-dominant,phenotype
R5500 T6894 T6890 pobj phenotype,with
R5501 T6895 T6894 prep in,phenotype
R5502 T6896 T6897 compound cpdm,mice
R5503 T6897 T6895 pobj mice,in
R5504 T6898 T6883 punct .,exhibited
R5580 T6974 T6966 advcl launch,release
R5581 T6975 T6974 cc and,launch
R5582 T6976 T6974 conj amplify,launch
R5583 T6977 T6979 det an,response
R5584 T6978 T6979 amod inflammatory,response
R5585 T6979 T6976 dobj response,amplify
R5586 T6980 T6982 nmod [,]
R5587 T6981 T6982 nummod 32,]
R5588 T6982 T6979 appos ],response
R5589 T6983 T6958 punct .,be
R5590 T6984 T6986 amod Such,inflammation
R5591 T6985 T6986 amod sterile,inflammation
R5592 T6986 T6989 nsubjpass inflammation,initiated
R5593 T6987 T6989 aux could,initiated
R5594 T6988 T6989 auxpass be,initiated
R5595 T6989 T6989 ROOT initiated,initiated
R5596 T6990 T6989 cc and,initiated
R5597 T6991 T6989 conj take,initiated
R5598 T6992 T6991 dobj place,take
R5599 T6993 T6991 prep in,take
R5600 T6994 T6995 det all,organs
R5601 T6995 T6993 pobj organs,in
R5602 T6996 T6995 cc and,organs
R5603 T6997 T6995 conj tissues,organs
R5604 T6998 T6997 acl affected,tissues
R5605 T6999 T6998 prep in,affected
R5606 T7000 T7001 compound cpdm,mice
R5607 T7001 T6999 pobj mice,in
R5608 T7002 T6991 punct ",",take
R5609 T7003 T7004 advmod thus,causing
R5610 T7004 T6991 advcl causing,take
R5611 T7005 T7007 amod multi-organ,disorders
R5612 T7006 T7007 amod inflammatory,disorders
R5613 T7007 T7004 dobj disorders,causing
R5614 T7008 T6989 punct .,initiated
R5615 T7009 T7010 det This,hypothesis
R5616 T7010 T7012 nsubjpass hypothesis,supported
R5617 T7011 T7012 auxpass is,supported
R5618 T7012 T7012 ROOT supported,supported
R5619 T7013 T7012 agent by,supported
R5620 T7014 T7016 amod several,studies
R5621 T7015 T7016 amod recent,studies
R5622 T7016 T7013 pobj studies,by
R5623 T7017 T7012 punct .,supported
R5624 T7018 T7022 nsubj Fibroblasts,are
R5625 T7019 T7018 prep of,Fibroblasts
R5626 T7020 T7021 amod cpdm,mice
R5627 T7021 T7019 pobj mice,of
R5628 T7022 T7022 ROOT are,are
R5629 T7023 T7024 advmod highly,sensitive
R5630 T7024 T7022 acomp sensitive,are
R5631 T7025 T7024 prep to,sensitive
R5632 T7026 T7028 amod TNFα-induced,death
R5633 T7027 T7028 compound cell,death
R5634 T7028 T7025 pobj death,to
R5635 T7029 T7028 cc and,death
R5636 T7030 T7032 det the,phenotype
R5637 T7031 T7032 compound cpdm,phenotype
R5638 T7032 T7028 conj phenotype,death
R5639 T7033 T7036 aux can,rescued
R5640 T7034 T7036 auxpass be,rescued
R5641 T7035 T7036 advmod partially,rescued
R5642 T7036 T7022 conj rescued,are
R5643 T7037 T7036 agent by,rescued
R5644 T7038 T7037 pobj deletion,by
R5645 T7039 T7038 prep of,deletion
R5646 T7040 T7041 compound TNF,[
R5647 T7041 T7043 nmod [,]
R5648 T7042 T7043 nummod 7,]
R5649 T7043 T7039 pobj ],of
R5650 T7044 T7043 punct ",",]
R5651 T7045 T7047 nmod [,]
R5652 T7046 T7047 nummod 8,]
R5653 T7047 T7043 conj ],]
R5654 T7048 T7036 punct ",",rescued
R5861 T7255 T7254 punct ",",NF-κB
R5862 T7256 T7254 appos TBK/IRF3,NF-κB
R5863 T7257 T7254 punct ",",NF-κB
R5864 T7258 T7254 cc and,NF-κB
R5865 T7259 T7254 conj MAPK,NF-κB
R5866 T7260 T7254 conj signaling,NF-κB
R5867 T7261 T7261 ROOT are,are
R5868 T7262 T7263 amod important,pathways
R5869 T7263 T7261 attr pathways,are
R5870 T7264 T7263 acl activated,pathways
R5871 T7265 T7264 agent by,activated
R5872 T7266 T7265 pobj LPS,by
R5873 T7267 T7264 cc or,activated
R5874 T7268 T7271 advmod poly,C
R5875 T7269 T7271 nsubj I,C
R5876 T7270 T7271 punct :,C
R5877 T7271 T7261 dep C,are
R5878 T7272 T7271 cc and,C
R5879 T7273 T7271 conj disruption,C
R5880 T7274 T7273 prep of,disruption
R5881 T7275 T7276 det either,pathway
R5882 T7276 T7274 pobj pathway,of
R5883 T7277 T7278 aux may,lead
R5884 T7278 T7261 advcl lead,are
R5885 T7279 T7278 prep to,lead
R5886 T7280 T7282 amod decreased,expression
R5887 T7281 T7282 compound cytokine,expression
R5888 T7282 T7279 pobj expression,to
R5889 T7283 T7261 punct .,are
R5715 T7109 T7110 nsubj that,exert
R5716 T7110 T7108 relcl exert,DAMPs
R5717 T7111 T7113 poss their,properties
R5718 T7112 T7113 compound pro-inflammatory,properties
R5719 T7113 T7110 dobj properties,exert
R5727 T7121 T7123 nmod [,]
R5807 T7201 T7199 pobj mice,in
R5835 T7229 T7226 punct -,demonstrated
R5836 T7230 T7226 cc and,demonstrated
R5837 T7231 T7232 amod IL12P40-deficient,mice
R5838 T7232 T7226 conj mice,demonstrated
R5839 T7233 T7235 nmod [,]
R5840 T7234 T7235 nummod 38,]
R5841 T7235 T7232 appos ],mice
R5842 T7236 T7235 punct ",",]
R5843 T7237 T7239 nmod [,]
R5844 T7238 T7239 nummod 39,]
R5845 T7239 T7235 conj ],]
R5846 T7240 T7232 cc and,mice
R5847 T7241 T7232 conj through,mice
R5848 T7242 T7243 amod clinical,studies
R5849 T7243 T7241 pobj studies,through
R5850 T7244 T7243 prep in,studies
R5851 T7245 T7246 amod human,patients
R5852 T7246 T7244 pobj patients,in
R5853 T7247 T7249 nmod [,]
R5854 T7248 T7249 nummod 40,]
R5855 T7249 T7243 appos ],studies
R5856 T7250 T7252 nmod [,]
R5857 T7251 T7252 nummod 42,]
R5858 T7252 T7249 appos ],]
R5859 T7253 T7226 punct .,demonstrated
R5860 T7254 T7261 nsubj NF-κB,are
R5936 T7330 T7326 pobj expression,for
R5937 T7331 T7296 punct .,stimulated
R5938 T7332 T7333 det These,results
R5939 T7333 T7334 nsubj results,seem
R5940 T7334 T7334 ROOT seem,seem
R5941 T7335 T7336 aux to,contradict
R5942 T7336 T7334 xcomp contradict,seem
R5943 T7337 T7339 det a,study
R5944 T7338 T7339 amod recent,study
R5945 T7339 T7336 dobj study,contradict
R5946 T7340 T7341 nsubj that,found
R5947 T7341 T7339 relcl found,study
R5948 T7342 T7343 amod increased,levels
R5949 T7343 T7341 dobj levels,found
R5950 T7344 T7343 prep of,levels
R5951 T7345 T7346 amod NF-κB,activation
R5952 T7346 T7344 pobj activation,of
R5953 T7347 T7346 cc and,activation
R5954 T7348 T7349 nummod IL1,transcription
R5955 T7349 T7346 conj transcription,activation
R5956 T7350 T7349 prep in,transcription
R5957 T7351 T7352 compound cpdm,mice
R5958 T7352 T7350 pobj mice,in
R5959 T7353 T7355 nmod [,]
R5960 T7354 T7355 nummod 43,]
R5961 T7355 T7352 appos ],mice
R5962 T7356 T7334 punct .,seem
R5963 T7357 T7358 det This,difference
R5964 T7358 T7362 nsubjpass difference,attributed
R5965 T7359 T7362 aux can,attributed
R5966 T7360 T7362 advmod probably,attributed
R5967 T7361 T7362 auxpass be,attributed
R5968 T7362 T7362 ROOT attributed,attributed
R5969 T7363 T7362 prep to,attributed
R5970 T7364 T7366 det the,cells
R5971 T7365 T7366 amod different,cells
R5972 T7366 T7363 pobj cells,to
R5973 T7367 T7366 cc and,cells
R5974 T7368 T7366 conj tissues,cells
R5975 T7369 T7368 acl used,tissues
R5976 T7370 T7369 prep in,used
R5977 T7371 T7372 det these,studies
R5978 T7372 T7370 pobj studies,in
R5979 T7373 T7362 punct ",",attributed
R5980 T7374 T7362 cc and,attributed
R5981 T7375 T7376 aux may,point
R5982 T7376 T7362 conj point,attributed
R5983 T7377 T7376 prep to,point
R5984 T7378 T7377 pobj cell,to
R5985 T7379 T7377 punct -,to
R5986 T7380 T7377 cc and,to
R5987 T7381 T7383 compound tissue,type
R5988 T7382 T7383 punct -,type
R5989 T7383 T7377 pobj type,to
R5990 T7384 T7385 amod specific,functions
R5991 T7385 T7383 dobj functions,type
R5992 T7386 T7385 prep of,functions
R5993 T7387 T7386 pobj SHARPIN,of
R5994 T7388 T7362 punct .,attributed
R5995 T7389 T7390 nsubj This,is
R5996 T7390 T7390 ROOT is,is
R5997 T7391 T7390 acomp consistent,is
R5998 T7392 T7391 prep with,consistent
R5999 T7393 T7394 amod recent,reports
R6000 T7394 T7392 pobj reports,with
R6001 T7395 T7394 prep of,reports
R6002 T7396 T7397 amod tissue-specific,effects
R6003 T7397 T7395 pobj effects,of
R6004 T7398 T7397 prep of,effects
R6005 T7399 T7400 nsubj NF-κB,signaling
R6006 T7400 T7398 pcomp signaling,of
R6007 T7401 T7403 nmod [,]
R6008 T7402 T7403 nummod 44,]
R6009 T7403 T7400 dobj ],signaling
R6010 T7404 T7390 punct .,is
R6086 T7480 T7480 ROOT indicates,indicates
R6087 T7481 T7499 mark that,is
R6088 T7482 T7483 det a,balance
R6089 T7483 T7499 nsubj balance,is
R6090 T7484 T7483 prep between,balance
R6091 T7485 T7487 det the,role
R6092 T7486 T7487 amod pro-inflammatory,role
R6093 T7487 T7484 pobj role,between
R6094 T7488 T7487 prep of,role
R6095 T7489 T7488 pobj NF-κB,of
R6096 T7490 T7487 prep in,role
R6097 T7491 T7490 pobj leukocytes,in
R6098 T7492 T7491 cc and,leukocytes
R6099 T7493 T7495 det the,role
R6100 T7494 T7495 amod anti-inflammatory,role
R6101 T7495 T7499 nsubj role,is
R6102 T7496 T7495 prep in,role
R6103 T7497 T7498 amod parenchymal,cells
R6104 T7498 T7496 pobj cells,in
R6105 T7499 T7480 ccomp is,indicates
R6106 T7500 T7499 acomp critical,is
R6107 T7501 T7500 prep in,critical
R6108 T7502 T7503 det the,maintenance
R6109 T7503 T7501 pobj maintenance,in
R6110 T7504 T7503 prep of,maintenance
R6111 T7505 T7506 compound tissue,homeostasis
R6112 T7506 T7504 pobj homeostasis,of
R6113 T7507 T7480 punct .,indicates
R6114 T7508 T7525 nsubj Experiments,help
R6115 T7509 T7508 prep with,Experiments
R6116 T7510 T7509 pobj mice,with
R6117 T7511 T7508 prep with,Experiments
R6118 T7512 T7511 pobj cell,with
R6119 T7513 T7508 punct -,Experiments
R6120 T7514 T7508 cc and,Experiments
R6121 T7515 T7516 amod tissue-specific,deletion
R6122 T7516 T7508 conj deletion,Experiments
R6123 T7517 T7516 prep of,deletion
R6124 T7518 T7517 pobj Sharpin,of
R5890 T7284 T7285 amod NF-κB,activation
R5891 T7285 T7288 nsubjpass activation,inhibited
R5892 T7286 T7288 auxpass was,inhibited
R5893 T7287 T7288 advmod selectively,inhibited
R5894 T7288 T7288 ROOT inhibited,inhibited
R5895 T7289 T7288 prep in,inhibited
R5896 T7290 T7289 pobj LPS,in
R5897 T7291 T7288 cc and,inhibited
R5898 T7292 T7296 advmod poly,stimulated
R5899 T7293 T7296 dep I,stimulated
R5900 T7294 T7296 punct :,stimulated
R5901 T7295 T7296 npadvmod C,stimulated
R5902 T7296 T7288 conj stimulated,inhibited
R5903 T7297 T7298 compound cpdm,BMDC
R5904 T7298 T7296 dobj BMDC,stimulated
R5905 T7299 T7296 prep compared,stimulated
R5906 T7300 T7299 prep to,compared
R5907 T7301 T7302 amod wild,type
R5908 T7302 T7300 pobj type,to
R5909 T7303 T7296 punct ",",stimulated
R5910 T7304 T7313 mark while,was
R5911 T7305 T7313 nsubj activation,was
R5912 T7306 T7305 prep of,activation
R5913 T7307 T7306 pobj TBK1/IRF3,of
R5914 T7308 T7305 punct ",",activation
R5915 T7309 T7305 conj ERK1/2,activation
R5916 T7310 T7309 punct ",",ERK1/2
R5917 T7311 T7309 cc or,ERK1/2
R5918 T7312 T7309 conj p38,ERK1/2
R5919 T7313 T7296 advcl was,stimulated
R5920 T7314 T7313 neg not,was
R5921 T7315 T7296 punct ",",stimulated
R5922 T7316 T7296 advcl indicating,stimulated
R5923 T7317 T7318 mark that,disrupted
R5924 T7318 T7316 ccomp disrupted,indicating
R5925 T7319 T7320 nsubj NF-κB,signaling
R5926 T7320 T7318 ccomp signaling,disrupted
R5927 T7321 T7320 prep in,signaling
R5928 T7322 T7323 compound cpdm,DC
R5929 T7323 T7321 pobj DC,in
R5930 T7324 T7320 ccomp is,signaling
R5931 T7325 T7324 acomp responsible,is
R5932 T7326 T7325 prep for,responsible
R5933 T7327 T7330 det the,expression
R5934 T7328 T7330 amod defective,expression
R5935 T7329 T7330 compound cytokine,expression
R6011 T7405 T7406 amod Ubiquitous,activation
R6012 T7406 T7406 ROOT activation,activation
R6013 T7407 T7406 prep of,activation
R6014 T7408 T7407 pobj NF-κB,of
R6015 T7409 T7406 prep by,activation
R6016 T7410 T7409 pcomp removing,by
R6017 T7411 T7410 dobj inhibitors,removing
R6018 T7412 T7413 amod such,as
R6019 T7413 T7411 prep as,inhibitors
R6020 T7414 T7413 pobj A20,as
R6021 T7415 T7414 cc and,A20
R6022 T7416 T7414 conj ITCH,A20
R6023 T7417 T7410 prep through,removing
R6024 T7418 T7419 amod genetic,manipulation
R6025 T7419 T7420 compound manipulation,results
R6026 T7420 T7417 pobj results,through
R6027 T7421 T7420 prep in,results
R6028 T7422 T7423 amod widespread,inflammation
R6029 T7423 T7421 pobj inflammation,in
R6030 T7424 T7423 punct ",",inflammation
R6031 T7425 T7420 amod consistent,results
R6032 T7426 T7425 prep with,consistent
R6033 T7427 T7428 det the,role
R6034 T7428 T7426 pobj role,with
R6035 T7429 T7428 prep of,role
R6036 T7430 T7429 pobj NF-κB,of
R6037 T7431 T7428 prep in,role
R6038 T7432 T7433 det the,production
R6039 T7433 T7431 pobj production,in
R6040 T7434 T7433 prep of,production
R6041 T7435 T7436 amod pro-inflammatory,mediators
R6042 T7436 T7434 pobj mediators,of
R6043 T7437 T7433 appos [,production
R6044 T7438 T7439 nummod 45,]
R6045 T7439 T7428 appos ],role
R6046 T7440 T7439 punct ",",]
R6047 T7441 T7443 nmod [,]
R6048 T7442 T7443 nummod 46,]
R6049 T7443 T7439 appos ],]
R6050 T7444 T7406 punct .,activation
R6051 T7445 T7445 ROOT However,However
R6052 T7446 T7445 pobj ",",However
R6053 T7447 T7448 amod selective,inhibition
R6054 T7448 T7477 nmod inhibition,]
R6055 T7449 T7448 prep of,inhibition
R6056 T7450 T7451 amod NF-κB,activation
R6057 T7451 T7449 pobj activation,of
R6058 T7452 T7451 prep in,activation
R6059 T7453 T7454 amod parenchymal,cells
R6060 T7454 T7452 pobj cells,in
R6061 T7455 T7454 prep of,cells
R6062 T7456 T7457 det the,skin
R6063 T7457 T7455 pobj skin,of
R6064 T7458 T7457 punct ",",skin
R6065 T7459 T7457 conj liver,skin
R6066 T7460 T7459 punct ",",liver
R6067 T7461 T7459 cc and,liver
R6068 T7462 T7463 compound intestine,results
R6069 T7463 T7448 conj results,inhibition
R6070 T7464 T7448 prep in,inhibition
R6071 T7465 T7466 amod chronic,inflammation
R6072 T7466 T7464 pobj inflammation,in
R6073 T7467 T7466 acl driven,inflammation
R6074 T7468 T7467 agent by,driven
R6075 T7469 T7471 amod NF-κB,leukocytes
R6076 T7470 T7471 amod competent,leukocytes
R6077 T7471 T7468 pobj leukocytes,by
R6078 T7472 T7474 nmod [,]
R6079 T7473 T7474 nummod 47,]
R6080 T7474 T7475 meta ],[
R6081 T7475 T7477 nmod [,]
R6082 T7476 T7477 nummod 50,]
R6083 T7477 T7446 dative ],","
R6084 T7478 T7477 punct .,]
R6085 T7479 T7480 nsubj This,indicates
R6161 T7555 T7554 cc or,populations
R6162 T7556 T7550 conj expression,change
R6163 T7557 T7556 prep of,expression
R6164 T7558 T7559 amod co-stimulatory,molecules
R6165 T7559 T7557 pobj molecules,of
R6166 T7560 T7547 prep on,was
R6167 T7561 T7560 pobj BMDC,on
R6168 T7562 T7547 punct .,was
R6169 T7563 T7565 amod Different,subunits
R6170 T7564 T7565 compound NF-κB,subunits
R6171 T7565 T7578 nsubj subunits,have
R6172 T7566 T7565 acl involved,subunits
R6173 T7567 T7566 prep in,involved
R6174 T7568 T7572 det the,branches
R6175 T7569 T7572 amod canonical,branches
R6176 T7570 T7569 cc and,canonical
R6177 T7571 T7569 conj non-canonical,canonical
R6178 T7572 T7567 pobj branches,in
R6179 T7573 T7572 prep of,branches
R6180 T7574 T7577 det the,pathway
R6181 T7575 T7577 nmod NF-κB,pathway
R6182 T7576 T7577 amod signaling,pathway
R6183 T7577 T7573 pobj pathway,of
R6184 T7578 T7578 ROOT have,have
R6185 T7579 T7580 amod distinct,functions
R6186 T7580 T7578 dobj functions,have
R6187 T7581 T7582 aux to,control
R6188 T7582 T7580 acl control,functions
R6189 T7583 T7584 amod specific,aspects
R6190 T7584 T7582 dobj aspects,control
R6191 T7585 T7584 prep of,aspects
R6192 T7586 T7587 compound DC,development
R6193 T7587 T7585 pobj development,of
R6194 T7588 T7587 cc and,development
R6195 T7589 T7582 conj function,control
R6196 T7590 T7592 nmod [,]
R6197 T7591 T7592 nummod 51,]
R6125 T7519 T7518 punct ",",Sharpin
R6126 T7520 T7521 advmod currently,under
R6127 T7521 T7516 prep under,deletion
R6128 T7522 T7521 pobj way,under
R6129 T7523 T7525 punct ",",help
R6130 T7524 T7525 aux will,help
R6131 T7525 T7525 ROOT help,help
R6132 T7526 T7527 aux to,elucidate
R6133 T7527 T7525 xcomp elucidate,help
R6134 T7528 T7529 det the,role
R6135 T7529 T7527 dobj role,elucidate
R6136 T7530 T7529 prep of,role
R6137 T7531 T7530 pobj SHARPIN,of
R6138 T7532 T7529 prep in,role
R6139 T7533 T7532 pobj inflammation,in
R6140 T7534 T7525 punct .,help
R6141 T7535 T7547 prep Despite,was
R6142 T7536 T7538 amod defective,activation
R6143 T7537 T7538 compound NF-κB,activation
R6144 T7538 T7535 pobj activation,Despite
R6145 T7539 T7538 prep in,activation
R6146 T7540 T7541 det the,absence
R6147 T7541 T7539 pobj absence,in
R6148 T7542 T7541 prep of,absence
R6149 T7543 T7544 compound Sharpin,expression
R6150 T7544 T7542 pobj expression,of
R6151 T7545 T7547 punct ",",was
R6152 T7546 T7547 expl there,was
R6153 T7547 T7547 ROOT was,was
R6154 T7548 T7550 det no,change
R6155 T7549 T7550 amod significant,change
R6156 T7550 T7547 attr change,was
R6157 T7551 T7550 prep in,change
R6158 T7552 T7554 amod splenic,populations
R6159 T7553 T7554 compound DC,populations
R6160 T7554 T7551 pobj populations,in
R6311 T7705 T7706 compound TLR,expression
R6312 T7706 T7703 pobj expression,of
R6313 T7707 T7706 prep on,expression
R6314 T7708 T7709 compound target,cells
R6315 T7709 T7707 pobj cells,on
R6316 T7710 T7687 punct .,is
R6317 T7711 T7712 nsubj LPS,engages
R6318 T7712 T7730 ccomp engages,triggers
R6319 T7713 T7712 dobj TLR4,engages
R6320 T7714 T7715 aux to,activate
R6321 T7715 T7712 advcl activate,engages
R6322 T7716 T7719 amod MYD88-dependent,pathways
R6323 T7717 T7716 cc and,MYD88-dependent
R6324 T7718 T7716 conj TRIF-dependent,MYD88-dependent
R6325 T7719 T7715 dobj pathways,activate
R6326 T7720 T7712 punct ",",engages
R6327 T7721 T7723 mark whereas,stimulated
R6328 T7722 T7723 nummod TLR3,stimulated
R6329 T7723 T7712 advcl stimulated,engages
R6330 T7724 T7723 agent by,stimulated
R6331 T7725 T7724 pobj poly,by
R6332 T7726 T7725 nummod I,poly
R6333 T7727 T7730 punct :,triggers
R6334 T7728 T7730 nsubj C,triggers
R6335 T7729 T7730 advmod only,triggers
R6336 T7730 T7730 ROOT triggers,triggers
R6337 T7731 T7732 nsubj TRIF-dependent,signaling
R6338 T7732 T7730 ccomp signaling,triggers
R6339 T7733 T7735 nmod [,]
R6340 T7734 T7735 nummod 53,]
R6341 T7735 T7732 dobj ],signaling
R6342 T7736 T7730 punct .,triggers
R6343 T7737 T7740 det The,activation
R6344 T7738 T7740 amod defective,activation
R6345 T7739 T7740 compound NF-κB,activation
R6346 T7740 T7744 nsubj activation,suggests
R6347 T7741 T7740 prep by,activation
R6348 T7742 T7743 det both,stimuli
R6349 T7743 T7741 pobj stimuli,by
R6350 T7744 T7744 ROOT suggests,suggests
R6351 T7745 T7749 mark that,interferes
R6352 T7746 T7748 det the,mutation
R6353 T7747 T7748 compound Sharpin,mutation
R6354 T7748 T7749 nsubj mutation,interferes
R6355 T7749 T7744 ccomp interferes,suggests
R6356 T7750 T7749 prep with,interferes
R6357 T7751 T7753 det the,adaptors
R6358 T7752 T7753 compound protein,adaptors
R6359 T7753 T7750 pobj adaptors,with
R6360 T7754 T7753 cc or,adaptors
R6361 T7755 T7753 conj kinases,adaptors
R6362 T7756 T7753 acl shared,adaptors
R6363 T7757 T7756 agent by,shared
R6364 T7758 T7760 det both,pathways
R6365 T7759 T7760 amod signaling,pathways
R6366 T7760 T7757 pobj pathways,by
R6367 T7761 T7760 punct ",",pathways
R6368 T7762 T7763 amod such,as
R6369 T7763 T7760 prep as,pathways
R6370 T7764 T7763 pobj RIP1,as
R6371 T7765 T7764 cc and,RIP1
R6372 T7766 T7764 conj TRAF6,RIP1
R6373 T7767 T7769 nmod [,]
R6374 T7768 T7769 nummod 18,]
R6375 T7769 T7760 appos ],pathways
R6376 T7770 T7744 punct .,suggests
R6377 T7771 T7772 amod Recent,studies
R6378 T7772 T7773 nsubj studies,demonstrated
R6379 T7773 T7773 ROOT demonstrated,demonstrated
R6380 T7774 T7776 mark that,interacts
R6381 T7775 T7776 nsubj SHARPIN,interacts
R6382 T7776 T7773 ccomp interacts,demonstrated
R6383 T7777 T7776 prep with,interacts
R6393 T7787 T7786 prep on,activity
R6394 T7788 T7789 amod NF-κB,signaling
R6470 T7864 T7863 prep of,role
R6471 T7865 T7864 pobj SHARPIN,of
R6472 T7866 T7863 prep in,role
R6473 T7867 T7868 det the,production
R6474 T7868 T7866 pobj production,in
R6475 T7869 T7868 prep of,production
R6476 T7870 T7871 amod pro-inflammatory,mediators
R6477 T7871 T7869 pobj mediators,of
R6478 T7872 T7871 cc and,mediators
R6479 T7873 T7874 amod TLR-induced,NF-κB
R6480 T7874 T7871 conj NF-κB,mediators
R6481 T7875 T7860 advcl signaling,identified
R6482 T7876 T7860 punct .,identified
R6483 T7877 T7880 det The,ability
R6484 T7878 T7880 amod impaired,ability
R6485 T7879 T7880 compound Th1-stimulating,ability
R6486 T7880 T7885 nsubj ability,account
R6487 T7881 T7880 prep of,ability
R6488 T7882 T7883 amod Sharpin-deficient,DC
R6489 T7883 T7881 pobj DC,of
R6490 T7884 T7885 aux may,account
R6491 T7885 T7885 ROOT account,account
R6492 T7886 T7885 prep for,account
R6493 T7887 T7890 det the,phenotype
R6494 T7888 T7890 amod Th2-dominant,phenotype
R6495 T7889 T7890 amod inflammatory,phenotype
R6496 T7890 T7886 pobj phenotype,for
R6497 T7891 T7890 prep of,phenotype
R6498 T7892 T7893 compound cpdm,mice
R6499 T7893 T7891 pobj mice,of
R6500 T7894 T7885 punct .,account
R6501 T7895 T7896 det The,balance
R6502 T7896 T7902 nsubj balance,is
R6198 T7592 T7589 dobj ],function
R6199 T7593 T7592 punct ",",]
R6200 T7594 T7592 conj [,]
R6201 T7595 T7596 nummod 52,]
R6202 T7596 T7592 conj ],]
R6203 T7597 T7578 punct .,have
R6204 T7598 T7600 det The,pathway
R6205 T7599 T7600 amod non-canonical,pathway
R6206 T7600 T7608 nsubj pathway,appears
R6207 T7601 T7600 punct (,pathway
R6208 T7602 T7603 amod p100,processing
R6209 T7603 T7600 appos processing,pathway
R6210 T7604 T7605 aux to,produce
R6211 T7605 T7603 relcl produce,processing
R6212 T7606 T7605 dobj p52,produce
R6213 T7607 T7600 punct ),pathway
R6214 T7608 T7608 ROOT appears,appears
R6215 T7609 T7610 aux to,be
R6216 T7610 T7608 xcomp be,appears
R6217 T7611 T7610 acomp intact,be
R6218 T7612 T7611 prep in,intact
R6219 T7613 T7614 amod SHARPIN-deficient,cells
R6220 T7614 T7612 pobj cells,in
R6221 T7615 T7617 nmod [,]
R6222 T7616 T7617 nummod 7,]
R6223 T7617 T7622 nsubj ],suggesting
R6224 T7618 T7617 punct ",",]
R6225 T7619 T7621 nmod [,]
R6226 T7620 T7621 nummod 9,]
R6227 T7621 T7617 conj ],]
R6228 T7622 T7608 advcl suggesting,appears
R6229 T7623 T7628 mark that,is
R6230 T7624 T7627 det the,p52/RELB
R6231 T7625 T7627 amod NF-κB,p52/RELB
R6232 T7626 T7627 compound heterodimer,p52/RELB
R6233 T7627 T7628 nsubj p52/RELB,is
R6234 T7628 T7622 ccomp is,suggesting
R6235 T7629 T7628 acomp sufficient,is
R6236 T7630 T7631 aux to,maintain
R6237 T7631 T7629 xcomp maintain,sufficient
R6238 T7632 T7634 det the,regulation
R6239 T7633 T7634 amod normal,regulation
R6240 T7634 T7631 dobj regulation,maintain
R6241 T7635 T7634 prep of,regulation
R6242 T7636 T7637 compound DC,homeostasis
R6243 T7637 T7635 pobj homeostasis,of
R6244 T7638 T7637 cc and,homeostasis
R6245 T7639 T7637 conj maturation,homeostasis
R6246 T7640 T7608 punct .,appears
R6247 T7641 T7643 det The,basis
R6248 T7642 T7643 amod molecular,basis
R6249 T7643 T7649 nsubj basis,causes
R6250 T7644 T7649 prep by,causes
R6251 T7645 T7644 pobj which,by
R6252 T7646 T7648 det the,mutation
R6253 T7647 T7648 compound Sharpin,mutation
R6254 T7648 T7649 nsubj mutation,causes
R6255 T7649 T7649 ROOT causes,causes
R6256 T7650 T7652 amod reduced,activation
R6257 T7651 T7652 amod NF-κB,activation
R6258 T7652 T7655 nsubj activation,remains
R6259 T7653 T7652 prep in,activation
R6260 T7654 T7653 pobj BMDC,in
R6261 T7655 T7649 ccomp remains,causes
R6262 T7656 T7658 aux to,determined
R6263 T7657 T7658 auxpass be,determined
R6264 T7658 T7655 xcomp determined,remains
R6265 T7659 T7655 punct .,remains
R6266 T7660 T7660 ROOT LPS,LPS
R6267 T7661 T7660 cc and,LPS
R6268 T7662 T7660 conj poly,LPS
R6269 T7663 T7668 nsubj I,are
R6270 T7664 T7668 punct :,are
R6271 T7665 T7666 npadvmod C,used
R6272 T7666 T7668 csubj used,are
R6273 T7667 T7666 advmod here,used
R6274 T7668 T7668 ROOT are,are
R6275 T7669 T7670 amod well-defined,ligands
R6276 T7670 T7668 attr ligands,are
R6277 T7671 T7673 nsubj that,engage
R6278 T7672 T7673 advmod specifically,engage
R6279 T7673 T7670 relcl engage,ligands
R6280 T7674 T7673 dobj TLR4,engage
R6281 T7675 T7674 cc and,TLR4
R6282 T7676 T7674 conj TLR3,TLR4
R6283 T7677 T7673 punct ",",engage
R6284 T7678 T7673 advmod respectively,engage
R6285 T7679 T7668 punct .,are
R6286 T7680 T7682 det The,profile
R6287 T7681 T7682 compound expression,profile
R6288 T7682 T7687 nsubj profile,is
R6289 T7683 T7682 prep of,profile
R6290 T7684 T7683 pobj surface,of
R6291 T7685 T7684 nummod TLR4,surface
R6292 T7686 T7682 appos complexes,profile
R6293 T7687 T7687 ROOT is,is
R6294 T7688 T7687 acomp similar,is
R6295 T7689 T7688 prep between,similar
R6296 T7690 T7689 pobj WT,between
R6297 T7691 T7690 cc and,WT
R6298 T7692 T7693 compound cpdm,BMDC
R6299 T7693 T7690 conj BMDC,WT
R6300 T7694 T7687 advcl suggesting,is
R6301 T7695 T7699 mark that,is
R6302 T7696 T7698 amod defective,activation
R6303 T7697 T7698 compound NF-κB,activation
R6304 T7698 T7699 nsubj activation,is
R6305 T7699 T7694 ccomp is,suggesting
R6306 T7700 T7699 neg not,is
R6307 T7701 T7702 det a,result
R6308 T7702 T7699 attr result,is
R6309 T7703 T7702 prep of,result
R6310 T7704 T7706 compound differential,expression
R6395 T7789 T7787 pobj signaling,on
R6396 T7790 T7791 compound players,RIP1
R6397 T7791 T7796 nmod RIP1,]
R6398 T7792 T7791 cc and,RIP1
R6399 T7793 T7794 compound NEMO,[
R6400 T7794 T7791 conj [,RIP1
R6401 T7795 T7796 nummod 7,]
R6402 T7796 T7789 dobj ],signaling
R6403 T7797 T7796 punct ",",]
R6404 T7798 T7800 det an,step
R6405 T7799 T7800 amod essential,step
R6406 T7800 T7796 appos step,]
R6407 T7801 T7800 prep for,step
R6408 T7802 T7805 amod intact,activation
R6409 T7803 T7805 amod TNFα-stimulated,activation
R6410 T7804 T7805 compound NF-κB,activation
R6411 T7805 T7801 pobj activation,for
R6412 T7806 T7773 punct .,demonstrated
R6413 T7807 T7810 det The,mutation
R6414 T7808 T7810 nmod Sharpin,mutation
R6415 T7809 T7810 amod null,mutation
R6416 T7810 T7811 nsubj mutation,disrupts
R6417 T7811 T7811 ROOT disrupts,disrupts
R6418 T7812 T7814 det the,process
R6419 T7813 T7814 compound ubiquitylation,process
R6420 T7814 T7811 dobj process,disrupts
R6421 T7815 T7811 cc and,disrupts
R6422 T7816 T7811 conj abrogates,disrupts
R6423 T7817 T7819 det the,NF-κB
R6424 T7818 T7819 amod TNFα-induced,NF-κB
R6425 T7819 T7820 nsubj NF-κB,signaling
R6426 T7820 T7816 ccomp signaling,abrogates
R6427 T7821 T7820 dobj pathway,signaling
R6428 T7822 T7811 punct .,disrupts
R6429 T7823 T7828 mark Since,share
R6430 T7824 T7828 nsubj TNFR,share
R6431 T7825 T7824 cc and,TNFR
R6432 T7826 T7824 conj TLR,TNFR
R6433 T7827 T7828 advmod partially,share
R6434 T7828 T7839 advcl share,hold
R6435 T7829 T7832 poss their,cascades
R6436 T7830 T7832 amod downstream,cascades
R6437 T7831 T7832 compound signaling,cascades
R6438 T7832 T7828 dobj cascades,share
R6439 T7833 T7839 punct ",",hold
R6503 T7897 T7896 prep between,balance
R6504 T7898 T7897 pobj Th1,between
R6505 T7899 T7898 cc and,Th1
R6506 T7900 T7898 conj Th2,Th1
R6507 T7901 T7902 nsubj differentiation,is
R6508 T7902 T7902 ROOT is,is
R6509 T7903 T7902 acomp critical,is
R6510 T7904 T7903 prep for,critical
R6511 T7905 T7906 amod immune,homeostasis
R6512 T7906 T7904 pobj homeostasis,for
R6513 T7907 T7902 punct .,is
R6514 T7908 T7910 det A,understanding
R6515 T7909 T7910 amod better,understanding
R6516 T7910 T7918 nsubj understanding,help
R6517 T7911 T7910 prep of,understanding
R6518 T7912 T7916 advmod how,maintained
R6519 T7913 T7914 amod such,balance
R6520 T7914 T7916 nsubjpass balance,maintained
R6521 T7915 T7916 auxpass is,maintained
R6522 T7916 T7918 acomp maintained,help
R6523 T7917 T7918 aux will,help
R6524 T7918 T7918 ROOT help,help
R6525 T7919 T7918 xcomp design,help
R6526 T7920 T7921 compound cytokine,treatment
R6527 T7921 T7919 dobj treatment,design
R6528 T7922 T7921 prep for,treatment
R6529 T7923 T7924 amod human,diseases
R6530 T7924 T7922 pobj diseases,for
R6531 T7925 T7921 prep with,treatment
R6532 T7926 T7928 amod Th2-biased,secretion
R6533 T7927 T7928 compound cytokine,secretion
R6534 T7928 T7925 pobj secretion,with
R6535 T7929 T7928 amod similar,secretion
R6536 T7930 T7929 prep to,similar
R6537 T7931 T7933 det the,cpdm
R6538 T7932 T7933 compound mouse,cpdm
R6539 T7933 T7930 pobj cpdm,to
R6540 T7934 T7933 punct ",",cpdm
R6541 T7935 T7936 amod such,as
R6542 T7936 T7929 prep as,similar
R6543 T7937 T7936 pobj allergies,as
R6544 T7938 T7937 cc and,allergies
R7885 T9636 T9636 ROOT Mice,Mice
R7890 T9641 T9640 pobj C57BL/KaLawRij-Sharpincpdm/RijSunJ,of
R7891 T9642 T9651 punct (,obtained
R7892 T9643 T9649 nmod JR,mice
R7893 T9644 T9645 nmod #,7599
R7894 T9645 T9643 nummod 7599,JR
R7895 T9646 T9643 punct ),JR
R7896 T9647 T9643 cc and,JR
R7897 T9648 T9649 compound WT,mice
R7898 T9649 T9651 nsubjpass mice,obtained
R7899 T9650 T9651 auxpass were,obtained
R7900 T9651 T9636 parataxis obtained,Mice
R7901 T9652 T9651 prep from,obtained
R7902 T9653 T9655 det The,Laboratory
R7903 T9654 T9655 compound Jackson,Laboratory
R7904 T9655 T9652 pobj Laboratory,from
R7905 T9656 T9655 punct (,Laboratory
R7906 T9657 T9658 compound Bar,Harbor
R7907 T9658 T9655 appos Harbor,Laboratory
R7908 T9659 T9658 punct ",",Harbor
R7909 T9660 T9658 appos ME,Harbor
R7910 T9661 T9658 punct ),Harbor
R7911 T9662 T9651 cc and,obtained
R7912 T9663 T9664 auxpass were,maintained
R7913 T9664 T9651 conj maintained,obtained
R7914 T9665 T9664 prep in,maintained
R7915 T9666 T9668 det a,facility
R7916 T9667 T9668 compound barrier,facility
R7917 T9668 T9665 pobj facility,in
R7918 T9669 T9651 punct .,obtained
R7919 T9670 T9672 amod Normal,controls
R7920 T9671 T9672 compound littermate,controls
R7921 T9672 T9673 nsubj controls,were
R7922 T9673 T9673 ROOT were,were
R7923 T9674 T9675 preconj either,+
R7924 T9675 T9673 conj +,were
R7925 T9676 T9677 nummod /,+
R7926 T9677 T9675 nsubj +,+
R7927 T9678 T9677 cc or,+
R7928 T9679 T9681 nummod +,Sharpincpdm
R7929 T9680 T9681 nummod /,Sharpincpdm
R7930 T9681 T9677 conj Sharpincpdm,+
R7931 T9682 T9675 punct .,+
R7932 T9683 T9685 det These,animals
R7933 T9684 T9685 compound control,animals
R7934 T9685 T9686 nsubj animals,were
R7935 T9686 T9686 ROOT were,were
R7936 T9687 T9688 advmod phenotypically,indistinguishable
R7937 T9688 T9686 acomp indistinguishable,were
R7938 T9689 T9686 cc and,were
R7939 T9690 T9691 auxpass are,referred
R7940 T9691 T9686 conj referred,were
R7941 T9692 T9691 prep to,referred
R7942 T9693 T9691 prep as,referred
R7943 T9694 T9693 pobj WT,as
R7944 T9695 T9691 punct .,referred
R7945 T9696 T9697 compound Sex-matched,WT
R7946 T9697 T9702 nsubjpass WT,used
R7947 T9698 T9697 cc and,WT
R7948 T9699 T9700 amod mutant,mice
R7949 T9700 T9697 conj mice,WT
R7950 T9701 T9702 auxpass were,used
R7951 T9702 T9702 ROOT used,used
R7952 T9703 T9702 prep at,used
R7953 T9704 T9703 pobj 6,at
R7954 T9705 T9706 nummod 10,weeks
R7955 T9706 T9704 appos weeks,6
R7956 T9707 T9706 prep of,weeks
R7957 T9708 T9707 pobj age,of
R7958 T9709 T9702 punct .,used
R7959 T9710 T9717 prep For,crossed
R7960 T9711 T9712 det some,experiments
R7961 T9712 T9710 pobj experiments,For
R7962 T9713 T9717 punct ",",crossed
R8190 T9975 T9974 punct (,DNA
R8191 T9976 T9974 appos cDNA,DNA
R8192 T9977 T9974 punct ),DNA
R8193 T9978 T9974 prep of,DNA
R8194 T9979 T9982 det the,gene
R8195 T9980 T9982 compound mouse,gene
R8196 T9981 T9982 compound Sharpin,gene
R8197 T9982 T9978 pobj gene,of
R8198 T9983 T9984 auxpass was,cloned
R8199 T9984 T9984 ROOT cloned,cloned
R8200 T9985 T9984 cc and,cloned
R8201 T9986 T9984 conj amplified,cloned
R8202 T9987 T9986 prep from,amplified
R8203 T9988 T9990 compound RAW264,RNA
R8204 T9989 T9990 compound .7,RNA
R8205 T9990 T9991 compound RNA,extracts
R8206 T9991 T9987 pobj extracts,from
R8207 T9992 T9984 punct .,cloned
R8208 T9993 T9995 det The,sequences
R8209 T9994 T9995 compound primer,sequences
R8210 T9995 T9996 nsubj sequences,were
R8211 T9996 T10027 ccomp were,A
R8212 T9997 T9996 advmod forward,were
R8213 T9998 T9996 punct ",",were
R8214 T9999 T10011 nummod 5,GGT
R8215 T10000 T10002 punct ′,CC
R8216 T10001 T10002 punct -,CC
R8217 T10002 T10004 compound CC,GCG
R8218 T10003 T10004 compound ATG,GCG
R8219 T10004 T10011 compound GCG,GGT
R8220 T10005 T10011 compound ATG,GGT
R8221 T10006 T10011 compound TCG,GGT
R8222 T10007 T10011 compound CCG,GGT
R8223 T10008 T10011 compound CCC,GGT
R8224 T10009 T10011 compound GCC,GGT
R8225 T10010 T10011 compound GGC,GGT
R8226 T10011 T9996 attr GGT,were
R8227 T10012 T10027 punct ;,A
R6440 T7834 T7837 det a,regulation
R6441 T7835 T7837 amod similar,regulation
R6442 T7836 T7837 amod ubiquitin-mediated,regulation
R6443 T7837 T7839 nsubj regulation,hold
R6444 T7838 T7839 aux may,hold
R6445 T7839 T7839 ROOT hold,hold
R6446 T7840 T7839 acomp true,hold
R6447 T7841 T7839 prep for,hold
R6448 T7842 T7841 pobj SHARPIN,for
R6449 T7843 T7839 prep in,hold
R6450 T7844 T7843 pobj LPS,in
R6451 T7845 T7839 punct -,hold
R6452 T7846 T7839 cc and,hold
R6453 T7847 T7839 conj poly,hold
R6454 T7848 T7847 nsubj I,poly
R6455 T7849 T7847 punct :,poly
R6456 T7850 T7852 amod C-induced,activation
R6457 T7851 T7852 amod NF-κB,activation
R6458 T7852 T7847 appos activation,poly
R6459 T7853 T7839 punct .,hold
R6460 T7854 T7860 prep In,identified
R6461 T7855 T7854 pobj summary,In
R6462 T7856 T7860 punct ",",identified
R6463 T7857 T7859 det the,study
R6464 T7858 T7859 amod present,study
R6465 T7859 T7860 nsubj study,identified
R6466 T7860 T7860 ROOT identified,identified
R6467 T7861 T7863 det an,role
R6468 T7862 T7863 amod indispensible,role
R6469 T7863 T7860 dobj role,identified
R6545 T7939 T7940 amod hypereosinophilic,syndromes
R6546 T7940 T7937 conj syndromes,allergies
R6547 T7941 T7918 punct .,help
R7886 T9637 T9639 amod Specific-pathogen,colonies
R7887 T9638 T9639 amod free,colonies
R7888 T9639 T9636 dobj colonies,Mice
R7889 T9640 T9639 prep of,colonies
R7841 T9579 T9580 compound Ethics,Statement
R7842 T9580 T9583 compound Statement,work
R7843 T9581 T9583 compound All,work
R7858 T9596 T9597 compound Animal,Care
R7859 T9597 T9600 nmod Care,Committees
R7860 T9598 T9597 cc and,Care
R7861 T9599 T9600 compound Use,Committees
R7862 T9600 T9593 pobj Committees,by
R7863 T9601 T9585 punct .,carried
R7963 T9714 T9715 compound cpdm,mice
R7964 T9715 T9717 nsubjpass mice,crossed
R7965 T9716 T9717 auxpass were,crossed
R7966 T9717 T9717 ROOT crossed,crossed
R7967 T9718 T9717 prep with,crossed
R7968 T9719 T9720 amod transgenic,mice
R7969 T9720 T9718 pobj mice,with
R7970 T9721 T9717 prep with,crossed
R7971 T9722 T9725 det a,chromosome
R7972 T9723 T9725 amod bacterial,chromosome
R7973 T9724 T9725 amod artificial,chromosome
R7974 T9725 T9721 pobj chromosome,with
R7975 T9726 T9725 punct (,chromosome
R7976 T9727 T9725 appos BAC,chromosome
R7977 T9728 T9725 punct ),chromosome
R7978 T9729 T9717 advcl containing,crossed
R7979 T9730 T9732 det the,gene
R7980 T9731 T9732 compound Sharpin,gene
R7981 T9732 T9729 dobj gene,containing
R7982 T9733 T9738 punct (,1Sun/Sun
R7983 T9734 T9738 nmod FVB/NJ-Tg,1Sun/Sun
R7984 T9735 T9736 punct (,RP24-173I23
R7985 T9736 T9734 appos RP24-173I23,FVB/NJ-Tg
R7986 T9737 T9738 punct ),1Sun/Sun
R7987 T9738 T9732 appos 1Sun/Sun,gene
R7988 T9739 T9738 punct ",",1Sun/Sun
R7989 T9740 T9741 compound JR,#
R7990 T9741 T9742 nmod #,8279
R7991 T9742 T9729 npadvmod 8279,containing
R7992 T9743 T9717 punct ),crossed
R7993 T9744 T9717 punct .,crossed
R7994 T9745 T9746 det These,mice
R7995 T9746 T9748 nsubjpass mice,backcrossed
R7996 T9747 T9748 auxpass were,backcrossed
R7997 T9748 T9748 ROOT backcrossed,backcrossed
R7998 T9749 T9748 prep onto,backcrossed
R7999 T9750 T9752 det the,background
R8000 T9751 T9752 amod C57BL/KaLawRij-Sharpincpdm/RijSunJ,background
R8001 T9752 T9749 pobj background,onto
R8002 T9753 T9752 cc and,background
R8003 T9754 T9755 nummod N4,mice
R8004 T9755 T9752 conj mice,background
R8005 T9756 T9757 auxpass were,used
R8006 T9757 T9748 conj used,backcrossed
R8007 T9758 T9757 prep in,used
R8008 T9759 T9760 det the,experiments
R8009 T9760 T9758 pobj experiments,in
R8010 T9761 T9748 conj reported,backcrossed
R8011 T9762 T9761 advmod here,reported
R8012 T9763 T9748 punct .,backcrossed
R8013 T9764 T9766 det All,work
R8014 T9765 T9766 compound mouse,work
R8015 T9766 T9768 nsubjpass work,carried
R8016 T9767 T9768 auxpass was,carried
R8017 T9768 T9768 ROOT carried,carried
R8018 T9769 T9768 prt out,carried
R8019 T9770 T9768 prep in,carried
R8020 T9771 T9772 amod strict,accordance
R8021 T9772 T9770 pobj accordance,in
R8022 T9773 T9772 prep with,accordance
R8023 T9774 T9776 det the,protocols
R8024 T9775 T9776 amod approved,protocols
R8025 T9776 T9773 pobj protocols,with
R8026 T9777 T9776 prep by,protocols
R8027 T9778 T9784 det the,Committee
R8028 T9779 T9781 compound Institutional,Care
R8029 T9780 T9781 compound Animal,Care
R8030 T9781 T9784 nmod Care,Committee
R8031 T9782 T9781 cc and,Care
R8032 T9783 T9784 compound Use,Committee
R8033 T9784 T9777 pobj Committee,by
R8034 T9785 T9768 punct .,carried
R8184 T9969 T9969 ROOT Constructs,Constructs
R8185 T9970 T9969 cc and,Constructs
R8186 T9971 T9969 conj transfection,Constructs
R8187 T9972 T9974 det The,DNA
R8188 T9973 T9974 amod complementary,DNA
R8189 T9974 T9984 nsubjpass DNA,cloned
R8265 T10050 T10047 dobj protein,express
R8266 T10051 T10045 punct .,used
R8267 T10052 T10059 nsubjpass Cells,seeded
R8268 T10053 T10052 punct (,Cells
R8269 T10054 T10056 nummod 2,104
R8270 T10055 T10056 nummod ×,104
R8271 T10056 T10052 appos 104,Cells
R8272 T10057 T10052 punct ),Cells
R8273 T10058 T10059 auxpass were,seeded
R8274 T10059 T10059 ROOT seeded,seeded
R8275 T10060 T10059 prep in,seeded
R8276 T10061 T10062 npadvmod 96-well,treated
R8277 T10062 T10063 amod treated,plates
R8278 T10063 T10060 pobj plates,in
R8279 T10064 T10065 det the,day
R8280 T10065 T10063 npadvmod day,plates
R8281 T10066 T10059 prep before,seeded
R8282 T10067 T10066 pobj transfection,before
R8283 T10068 T10059 punct .,seeded
R8284 T10069 T10078 prep After,transfected
R8285 T10070 T10071 amod overnight,incubation
R8286 T10071 T10069 pobj incubation,After
R8287 T10072 T10078 punct ",",transfected
R8288 T10073 T10076 nummod 200,plasmids
R8289 T10074 T10076 compound ng,plasmids
R8290 T10075 T10076 compound pFLAG-SHARPIN,plasmids
R8291 T10076 T10078 nsubjpass plasmids,transfected
R8292 T10077 T10078 auxpass were,transfected
R8293 T10078 T10078 ROOT transfected,transfected
R8294 T10079 T10078 prep with,transfected
R8295 T10080 T10081 nummod 0.5,ul
R8296 T10081 T10082 compound ul,Lipofectamine
R8297 T10082 T10079 pobj Lipofectamine,with
R8228 T10013 T10027 advmod reverse,A
R8229 T10014 T10027 punct ",",A
R8230 T10015 T10027 nummod 5,A
R8231 T10016 T10027 punct ′,A
R8232 T10017 T10027 punct -,A
R8233 T10018 T10027 compound AAG,A
R8234 T10019 T10027 compound CTT,A
R8235 T10020 T10027 compound CTA,A
R8236 T10021 T10027 compound GGT,A
R8237 T10022 T10027 compound GGA,A
R8238 T10023 T10027 compound AGC,A
R8239 T10024 T10027 compound TGC,A
R8240 T10025 T10027 compound AGC,A
R8241 T10026 T10027 compound AAG,A
R8242 T10027 T10027 ROOT A,A
R8243 T10028 T10027 punct .,A
R8244 T10029 T10031 det The,cDNA
R8245 T10030 T10031 compound Sharpin,cDNA
R8246 T10031 T10033 nsubjpass cDNA,cloned
R8247 T10032 T10033 auxpass was,cloned
R8248 T10033 T10033 ROOT cloned,cloned
R8249 T10034 T10033 prep into,cloned
R8250 T10035 T10036 compound expression,vector
R8251 T10036 T10037 compound vector,pFLAG-CMV-2
R8252 T10037 T10034 pobj pFLAG-CMV-2,into
R8253 T10038 T10033 punct .,cloned
R8254 T10039 T10040 compound Murine,fibroblasts
R8255 T10040 T10045 nsubjpass fibroblasts,used
R8256 T10041 T10040 cc and,fibroblasts
R8257 T10042 T10040 conj macrophages,fibroblasts
R8258 T10043 T10045 auxpass were,used
R8259 T10044 T10045 auxpass were,used
R8260 T10045 T10045 ROOT used,used
R8261 T10046 T10047 aux to,express
R8262 T10047 T10045 xcomp express,used
R8263 T10048 T10050 compound recombinant,protein
R8264 T10049 T10050 compound SHARPIN,protein
R8510 T10339 T10344 nsubjpass Generation,developed
R8511 T10340 T10339 prep of,Generation
R8512 T10341 T10342 compound BMDC,BMDC
R8513 T10342 T10340 pobj BMDC,of
R8514 T10343 T10344 auxpass were,developed
R8515 T10344 T10344 ROOT developed,developed
R8516 T10345 T10347 mark as,reported
R8517 T10346 T10347 advmod previously,reported
R8518 T10347 T10344 advcl reported,developed
R8519 T10348 T10350 nmod [,]
R8520 T10349 T10350 nummod 54,]
R8521 T10350 T10347 nsubj ],reported
R8522 T10351 T10350 punct ",",]
R8523 T10352 T10354 nmod [,]
R8524 T10353 T10354 nummod 55,]
R8525 T10354 T10350 appos ],]
R8526 T10355 T10344 punct .,developed
R8527 T10356 T10358 nsubjpass Cells,collected
R8528 T10357 T10358 auxpass were,collected
R8529 T10358 T10358 ROOT collected,collected
R8530 T10359 T10358 prep after,collected
R8531 T10360 T10359 pobj 10,after
R8532 T10361 T10362 nummod 12,days
R8533 T10362 T10360 appos days,10
R8534 T10363 T10362 prep of,days
R8535 T10364 T10363 pobj culture,of
R8536 T10365 T10358 punct .,collected
R8537 T10366 T10368 det The,yield
R8538 T10367 T10368 compound cell,yield
R8539 T10368 T10369 nsubj yield,was
R8540 T10369 T10369 ROOT was,was
R8541 T10370 T10369 attr 2,was
R8542 T10371 T10374 nummod 3,cells/mouse
R8543 T10372 T10374 nmod ×,cells/mouse
R8544 T10373 T10374 nummod 107,cells/mouse
R8545 T10374 T10369 attr cells/mouse,was
R8546 T10375 T10374 prep with,cells/mouse
R8547 T10376 T10375 pobj 80,with
R8548 T10377 T10379 nummod 95,BMDC
R8549 T10378 T10377 quantmod %,95
R8550 T10379 T10376 appos BMDC,80
R8551 T10380 T10369 punct .,was
R8640 T10488 T10487 prep for,incubated
R8641 T10489 T10490 nummod 30,minutes
R8642 T10490 T10488 pobj minutes,for
R8643 T10491 T10490 prep on,minutes
R8644 T10492 T10491 pobj ice,on
R8645 T10493 T10490 prep with,minutes
R8646 T10494 T10496 compound Alex,anti-CD11c
R8647 T10495 T10496 compound Fluor-labeled,anti-CD11c
R8648 T10496 T10493 pobj anti-CD11c,with
R8698 T10546 T10545 acl incubated,cells
R8699 T10547 T10546 prep with,incubated
R8700 T10548 T10551 amod biotinylated,MD-2
R8701 T10549 T10551 amod anti-TLR4,MD-2
R8702 T10550 T10551 compound /,MD-2
R8703 T10551 T10547 pobj MD-2,with
R8704 T10552 T10553 auxpass were,washed
R8705 T10553 T10553 ROOT washed,washed
R8706 T10554 T10553 advmod twice,washed
R8707 T10555 T10553 cc and,washed
R8708 T10556 T10553 conj incubated,washed
R8709 T10557 T10556 prep for,incubated
R8710 T10558 T10559 nummod 30,minutes
R8711 T10559 T10557 pobj minutes,for
R8712 T10560 T10559 prep on,minutes
R8713 T10561 T10560 pobj ice,on
R8714 T10562 T10559 prep with,minutes
R8715 T10563 T10562 pobj avidin-PE,with
R8716 T10564 T10553 punct .,washed
R8717 T10565 T10566 aux To,isolate
R8718 T10566 T10573 advcl isolate,digested
R8719 T10567 T10568 amod splenic,DC
R8720 T10568 T10566 dobj DC,isolate
R8721 T10569 T10573 punct ",",digested
R8722 T10570 T10573 nsubjpass spleens,digested
R8723 T10571 T10573 auxpass were,digested
R8724 T10572 T10573 advmod collagenase,digested
R8725 T10573 T10573 ROOT digested,digested
R8726 T10574 T10573 cc and,digested
R8727 T10575 T10573 conj subject,digested
R8728 T10576 T10575 prep to,subject
R8729 T10577 T10579 compound Percoll,centrifugation
R8730 T10578 T10579 compound gradient,centrifugation
R8731 T10579 T10576 pobj centrifugation,to
R8732 T10580 T10573 punct .,digested
R8733 T10581 T10582 det The,bands
R8734 T10582 T10590 nsubjpass bands,collected
R8735 T10583 T10582 prep at,bands
R8736 T10584 T10585 det the,35
R8737 T10585 T10583 pobj 35,at
R8738 T10586 T10587 nummod 55,%
R8739 T10587 T10588 compound %,interface
R8740 T10588 T10590 nsubjpass interface,collected
R8741 T10589 T10590 auxpass were,collected
R8742 T10590 T10590 ROOT collected,collected
R8743 T10591 T10592 aux to,stain
R8744 T10592 T10590 xcomp stain,collected
R8745 T10593 T10592 prep with,stain
R8746 T10594 T10593 pobj PE-anti-CD11c,with
R8747 T10595 T10594 punct (,PE-anti-CD11c
R8748 T10596 T10594 appos HL3,PE-anti-CD11c
R8749 T10597 T10596 punct ",",HL3
R8750 T10598 T10596 conj BioLegend,HL3
R8751 T10599 T10594 punct ),PE-anti-CD11c
R8752 T10600 T10594 punct ",",PE-anti-CD11c
R8753 T10601 T10594 conj APC-anti-PDCA-1,PE-anti-CD11c
R8754 T10602 T10601 punct (,APC-anti-PDCA-1
R8755 T10603 T10601 appos 927,APC-anti-PDCA-1
R8298 T10083 T10082 nummod 2000,Lipofectamine
R8299 T10084 T10078 punct .,transfected
R8300 T10085 T10086 nummod 24,hours
R8301 T10086 T10087 npadvmod hours,later
R8302 T10087 T10091 advmod later,incubated
R8303 T10088 T10091 punct ",",incubated
R8304 T10089 T10091 nsubjpass cells,incubated
R8305 T10090 T10091 auxpass were,incubated
R8306 T10091 T10091 ROOT incubated,incubated
R8307 T10092 T10091 prep with,incubated
R8308 T10093 T10095 amod fresh,medium
R8309 T10094 T10095 compound culture,medium
R8310 T10095 T10092 pobj medium,with
R8311 T10096 T10091 punct .,incubated
R8312 T10097 T10104 prep After,lysed
R8313 T10098 T10100 det another,hours
R8314 T10099 T10100 nummod 24,hours
R8315 T10100 T10097 pobj hours,After
R8316 T10101 T10104 punct ",",lysed
R8317 T10102 T10104 nsubjpass cells,lysed
R8318 T10103 T10104 auxpass were,lysed
R8319 T10104 T10104 ROOT lysed,lysed
R8320 T10105 T10106 aux to,confirm
R8321 T10106 T10104 advcl confirm,lysed
R8322 T10107 T10109 det the,expression
R8323 T10108 T10109 compound FLAG-SHARPIN,expression
R8324 T10109 T10106 dobj expression,confirm
R8325 T10110 T10109 prep by,expression
R8326 T10111 T10110 pobj immunoblots,by
R8327 T10112 T10111 prep with,immunoblots
R8328 T10113 T10112 pobj anti-FLAG,with
R8329 T10114 T10104 punct .,lysed
R8330 T10115 T10120 nsubj Cells,transfected
R8331 T10116 T10115 prep with,Cells
R8332 T10117 T10118 det no,transfection
R8333 T10118 T10116 pobj transfection,with
R8334 T10119 T10115 cc and,Cells
R8335 T10120 T10126 nsubjpass transfected,used
R8336 T10121 T10120 prep with,transfected
R8337 T10122 T10124 amod empty,pFLAG-CMV-2
R8338 T10123 T10124 compound vector,pFLAG-CMV-2
R8339 T10124 T10121 pobj pFLAG-CMV-2,with
R8340 T10125 T10126 auxpass were,used
R8341 T10126 T10126 ROOT used,used
R8342 T10127 T10126 prep as,used
R8343 T10128 T10129 amod negative,control
R8344 T10129 T10127 pobj control,as
R8345 T10130 T10126 prep in,used
R8346 T10131 T10133 det all,experiments
R8347 T10132 T10133 compound transfection,experiments
R8348 T10133 T10130 pobj experiments,in
R8349 T10134 T10126 punct .,used
R8584 T10432 T10438 nsubj Phenotype,subsets
R8585 T10433 T10432 prep of,Phenotype
R8586 T10434 T10433 pobj BMDC,of
R8587 T10435 T10432 cc and,Phenotype
R8588 T10436 T10437 compound splenic,DC
R8589 T10437 T10432 conj DC,Phenotype
R8590 T10438 T10438 ROOT subsets,subsets
R8591 T10439 T10440 det The,phenotype
R8592 T10440 T10444 nsubjpass phenotype,determined
R8593 T10441 T10440 prep of,phenotype
R8594 T10442 T10441 pobj BMDC,of
R8595 T10443 T10444 auxpass was,determined
R8596 T10444 T10438 ccomp determined,subsets
R8597 T10445 T10444 advmod before,determined
R8598 T10446 T10445 cc and,before
R8599 T10447 T10445 conj after,before
R8600 T10448 T10450 nummod 24,culture
R8601 T10449 T10450 compound hour,culture
R8602 T10450 T10447 pobj culture,after
R8603 T10451 T10450 prep in,culture
R8604 T10452 T10453 det the,presence
R8605 T10453 T10451 pobj presence,in
R8606 T10454 T10453 prep of,presence
R8607 T10455 T10457 nummod 100,LPS
R8608 T10456 T10457 compound ng/mL,LPS
R8609 T10457 T10454 pobj LPS,of
R8610 T10458 T10438 punct .,subsets
R8611 T10459 T10460 det The,cells
R8612 T10460 T10462 nsubjpass cells,incubated
R8613 T10461 T10462 auxpass were,incubated
R8614 T10462 T10462 ROOT incubated,incubated
R8615 T10463 T10462 prep in,incubated
R8616 T10464 T10463 pobj PBS,in
R8617 T10465 T10462 prep with,incubated
R8618 T10466 T10467 nummod 0.1,%
R8619 T10467 T10485 nsubj %,washed
R8620 T10468 T10467 amod NaN3,%
R8621 T10469 T10468 punct ",",NaN3
R8622 T10470 T10471 nummod 1,%
R8623 T10471 T10472 compound %,BSA
R8624 T10472 T10485 nsubj BSA,washed
R8625 T10473 T10472 cc and,BSA
R8626 T10474 T10475 nummod 10,%
R8627 T10475 T10478 nmod %,serum
R8628 T10476 T10478 amod normal,serum
R8629 T10477 T10478 compound rabbit,serum
R8630 T10478 T10472 conj serum,BSA
R8631 T10479 T10478 prep for,serum
R8632 T10480 T10481 nummod 20,minutes
R8633 T10481 T10479 pobj minutes,for
R8634 T10482 T10481 prep on,minutes
R8635 T10483 T10482 pobj ice,on
R8636 T10484 T10478 punct ",",serum
R8637 T10485 T10462 conj washed,incubated
R8638 T10486 T10485 cc and,washed
R8639 T10487 T10485 conj incubated,washed
R8764 T10612 T10608 punct ",",FITC-anti-CD8α
R8765 T10613 T10614 compound BD,BioScience
R8766 T10614 T10608 appos BioScience,FITC-anti-CD8α
R8767 T10615 T10608 punct ),FITC-anti-CD8α
R8768 T10616 T10590 punct .,collected
R8769 T10617 T10618 det The,cells
R8770 T10618 T10620 nsubjpass cells,washed
R8771 T10619 T10620 auxpass were,washed
R8772 T10620 T10620 ROOT washed,washed
R8773 T10621 T10620 advmod twice,washed
R8774 T10622 T10620 punct ",",washed
R8775 T10623 T10620 conj fixed,washed
R8776 T10624 T10623 prep in,fixed
R8777 T10625 T10626 nummod 2,%
R8778 T10626 T10627 compound %,paraformaldehyde
R8779 T10627 T10624 pobj paraformaldehyde,in
R8780 T10628 T10623 punct ",",fixed
R8781 T10629 T10623 cc and,fixed
R8782 T10630 T10623 conj stored,fixed
R8783 T10631 T10630 prep at,stored
R8784 T10632 T10633 nummod 4,°
R8785 T10633 T10631 pobj °,at
R8786 T10634 T10630 npadvmod C,stored
R8787 T10635 T10623 conj until,fixed
R8788 T10636 T10635 pobj analysis,until
R8789 T10637 T10620 punct .,washed
R8790 T10638 T10639 compound Flow,cytometry
R8791 T10639 T10641 nsubjpass cytometry,performed
R8792 T10640 T10641 auxpass was,performed
R8793 T10641 T10641 ROOT performed,performed
R8794 T10642 T10641 prep in,performed
R8795 T10643 T10648 det an,instrument
R8796 T10644 T10648 nmod Excel,instrument
R8797 T10645 T10646 punct (,Coulter
R8798 T10646 T10644 appos Coulter,Excel
R8799 T10647 T10644 punct ),Excel
R8800 T10648 T10642 pobj instrument,in
R8801 T10649 T10641 punct .,performed
R8802 T10650 T10651 compound Dead,cells
R8803 T10651 T10653 nsubjpass cells,omitted
R8804 T10652 T10653 auxpass were,omitted
R8805 T10653 T10653 ROOT omitted,omitted
R8806 T10654 T10653 prep from,omitted
R8807 T10655 T10656 det the,analysis
R8808 T10656 T10654 pobj analysis,from
R8809 T10657 T10653 prep by,omitted
R8810 T10658 T10657 pcomp gating,by
R8811 T10659 T10658 prep on,gating
R8812 T10660 T10659 pobj forward,on
R8756 T10604 T10601 punct ",",APC-anti-PDCA-1
R8757 T10605 T10601 conj BioLegend,APC-anti-PDCA-1
R8758 T10606 T10605 punct ),BioLegend
R8759 T10607 T10605 cc and,BioLegend
R8760 T10608 T10605 conj FITC-anti-CD8α,BioLegend
R8761 T10609 T10608 punct (,FITC-anti-CD8α
R8762 T10610 T10608 appos 53,FITC-anti-CD8α
R8763 T10611 T10608 appos 6.7,FITC-anti-CD8α
R9081 T10967 T10968 compound BMDC-T,cell
R9082 T10968 T10982 nsubjpass cell,stimulated
R9083 T10969 T10968 prep in,cell
R9084 T10970 T10971 amod vitro,interaction
R9085 T10971 T10969 pobj interaction,in
R9086 T10972 T10968 npadvmod WT,cell
R9087 T10973 T10968 cc and,cell
R9088 T10974 T10975 compound cpdm,BMDC
R9089 T10975 T10968 conj BMDC,cell
R9090 T10976 T10975 punct (,BMDC
R9091 T10977 T10979 nummod 5,104
R9092 T10978 T10979 nummod ×,104
R9093 T10979 T10975 appos 104,BMDC
R9094 T10980 T10975 punct ),BMDC
R9095 T10981 T10982 auxpass were,stimulated
R9096 T10982 T10982 ROOT stimulated,stimulated
R9097 T10983 T10982 prep with,stimulated
R9098 T10984 T10983 pobj medium,with
R9099 T10985 T10984 punct ",",medium
R9100 T10986 T10987 nummod 1,µg
R9101 T10987 T10990 compound µg,LPS
R9102 T10988 T10990 compound /,LPS
R9103 T10989 T10990 compound mL,LPS
R9104 T10990 T10984 appos LPS,medium
R9105 T10991 T10990 punct ",",LPS
R9106 T10992 T10995 nummod 25,mL
R9107 T10993 T10995 compound µg,mL
R9108 T10994 T10995 compound /,mL
R9109 T10995 T10990 conj mL,LPS
R9110 T10996 T10995 cc and,mL
R9111 T10997 T10999 nummod 5,/
R9112 T10998 T10999 compound µg,/
R9113 T10999 T11001 compound /,Pam3CYS
R9114 T11000 T11001 compound mL,Pam3CYS
R9115 T11001 T10995 conj Pam3CYS,mL
R9116 T11002 T10982 punct .,stimulated
R9117 T11003 T11004 nummod 24,hours
R9118 T11004 T11005 npadvmod hours,later
R9119 T11005 T11009 advmod later,collected
R9120 T11006 T11009 punct ",",collected
R9121 T11007 T11009 nsubjpass cells,collected
R9122 T11008 T11009 auxpass were,collected
R9123 T11009 T11009 ROOT collected,collected
R9124 T11010 T11009 cc and,collected
R9125 T11011 T11009 conj washed,collected
R9126 T11012 T11011 prep with,washed
R9127 T11013 T11012 pobj PBS,with
R9128 T11014 T11009 punct .,collected
R9129 T11015 T11016 amod Allogeneic,naïve
R9130 T11016 T11020 compound naïve,cells
R9131 T11017 T11020 compound CD4,cells
R9132 T11018 T11020 compound +,cells
R9133 T11019 T11020 compound T,cells
R9134 T11020 T11021 nsubj cells,were
R9135 T11021 T11021 ROOT were,were
R9136 T11022 T11021 acomp isolated,were
R9137 T11023 T11022 prep from,isolated
R9138 T11024 T11023 pobj spleens,from
R9139 T11025 T11024 prep of,spleens
R9140 T11026 T11027 compound BALB/c,mice
R9141 T11027 T11025 pobj mice,of
R9217 T11103 T11104 nsubj controls,show
R9218 T11104 T11104 ROOT show,show
R9219 T11105 T11106 det no,production
R9220 T11106 T11104 dobj production,show
R9221 T11107 T11106 prep of,production
R9222 T11108 T11109 amod aforementioned,cytokines
R9223 T11109 T11107 pobj cytokines,of
R9224 T11110 T11104 punct .,show
R9396 T11358 T11359 compound RNA,expression
R9397 T11359 T11364 nsubjpass expression,cultured
R9398 T11360 T11359 prep by,expression
R9399 T11361 T11362 compound BMDC,BMDC
R9400 T11362 T11360 pobj BMDC,by
R9401 T11363 T11364 auxpass were,cultured
R9402 T11364 T11364 ROOT cultured,cultured
R9403 T11365 T11364 prep at,cultured
R9404 T11366 T11367 nummod 106,cells/mL
R9405 T11367 T11365 pobj cells/mL,at
R9406 T11368 T11364 prep in,cultured
R9407 T11369 T11370 nummod 10,mL
R9408 T11370 T11368 pobj mL,in
R9409 T11371 T11370 prep of,mL
R9410 T11372 T11374 amod RPMI-1640,medium
R9411 T11373 T11374 amod complete,medium
R9412 T11374 T11371 pobj medium,of
R9413 T11375 T11364 prep in,cultured
R9414 T11376 T11377 det the,presence
R9415 T11377 T11375 pobj presence,in
R9416 T11378 T11377 cc or,presence
R9417 T11379 T11377 conj absence,presence
R9418 T11380 T11379 prep of,absence
R9419 T11381 T11383 nummod 100,LPS
R9420 T11382 T11383 compound ng/mL,LPS
R9421 T11383 T11380 pobj LPS,of
R9422 T11384 T11383 cc or,LPS
R9423 T11385 T11387 quantmod 25,/
R9424 T11386 T11387 quantmod µg,/
R9425 T11387 T11383 conj /,LPS
R9426 T11388 T11389 compound mL,poly
R9427 T11389 T11390 dep poly,I
R9428 T11390 T11387 quantmod I,/
R9429 T11391 T11390 punct :,I
R9430 T11392 T11390 appos C,I
R9431 T11393 T11364 punct .,cultured
R9432 T11394 T11402 prep After,isolated
R9433 T11395 T11398 nummod 1,hours
R9434 T11396 T11395 cc and,1
R9435 T11397 T11395 conj 2,1
R9436 T11398 T11394 pobj hours,After
R9437 T11399 T11402 punct ",",isolated
R9438 T11400 T11402 nsubjpass RNA,isolated
R9439 T11401 T11402 auxpass was,isolated
R9440 T11402 T11402 ROOT isolated,isolated
R9441 T11403 T11402 prep with,isolated
R9442 T11404 T11406 amod TRI-reagent,Sigma
R9443 T11405 T11406 punct (,Sigma
R9444 T11406 T11403 pobj Sigma,with
R9445 T11407 T11402 punct ),isolated
R9446 T11408 T11402 prep according,isolated
R9447 T11409 T11408 prep to,according
R9448 T11410 T11411 det the,manufacturer
R9449 T11411 T11413 poss manufacturer,instructions
R9450 T11412 T11411 case 's,manufacturer
R9451 T11413 T11409 pobj instructions,to
R9452 T11414 T11402 punct .,isolated
R9453 T11415 T11416 det The,expression
R9454 T11416 T11428 nsubjpass expression,determined
R9455 T11417 T11416 prep of,expression
R9456 T11418 T11417 pobj Il6,of
R9457 T11419 T11418 punct ",",Il6
R9458 T11420 T11418 conj Il12p40,Il6
R9459 T11421 T11420 punct ",",Il12p40
R9460 T11422 T11420 conj Gmcsf,Il12p40
R9461 T11423 T11422 punct ",",Gmcsf
R9462 T11424 T11422 cc and,Gmcsf
R9463 T11425 T11426 compound Sharpin,mRNA
R9539 T11501 T11502 auxpass were,used
R9540 T11502 T11502 ROOT used,used
R9541 T11503 T11502 prep according,used
R9542 T11504 T11503 prep to,according
R9543 T11505 T11506 det the,manufacturer
R8813 T10661 T10660 cc and,forward
R8814 T10662 T10663 nummod 90,°
R8815 T10663 T10665 npadvmod °,scatter
R8816 T10664 T10665 amod light,scatter
R8817 T10665 T10660 conj scatter,forward
R8818 T10666 T10665 punct ",",scatter
R8819 T10667 T10665 cc and,scatter
R8820 T10668 T10669 nummod "10,000",cells
R8821 T10669 T10671 nsubjpass cells,analyzed
R8822 T10670 T10671 auxpass were,analyzed
R8823 T10671 T10653 conj analyzed,omitted
R8824 T10672 T10671 agent by,analyzed
R8825 T10673 T10674 compound FlowJo,software
R8826 T10674 T10672 pobj software,by
R8827 T10675 T10671 punct .,analyzed
R9142 T11028 T11022 parataxis by,isolated
R9143 T11029 T11031 amod negative,kit
R9144 T11030 T11031 compound selection,kit
R9145 T11031 T11028 pobj kit,by
R9146 T11032 T11031 punct (,kit
R9147 T11033 T11031 appos Invitrogen,kit
R9148 T11034 T11022 punct ),isolated
R9149 T11035 T11021 cc and,were
R9150 T11036 T11038 auxpass were,added
R9151 T11037 T11038 advmod then,added
R9152 T11038 T11021 conj added,were
R9153 T11039 T11038 prep at,added
R9154 T11040 T11042 nummod 2.5,105
R9155 T11041 T11042 nummod ×,105
R9156 T11042 T11039 pobj 105,at
R9157 T11043 T11038 cc and,added
R9158 T11044 T11038 conj co-cultured,added
R9159 T11045 T11044 prep with,co-cultured
R9160 T11046 T11047 amod activated,BMDC
R9161 T11047 T11045 pobj BMDC,with
R9162 T11048 T11021 punct .,were
R9163 T11049 T11055 prep After,collected
R9164 T11050 T11051 nummod 5,days
R9165 T11051 T11049 pobj days,After
R9166 T11052 T11055 punct ",",collected
R9167 T11053 T11055 nsubjpass supernatant,collected
R9168 T11054 T11055 auxpass was,collected
R9169 T11055 T11055 ROOT collected,collected
R9170 T11056 T11055 cc and,collected
R9171 T11057 T11058 det the,secretion
R9172 T11058 T11055 conj secretion,collected
R9173 T11059 T11058 prep of,secretion
R9174 T11060 T11059 pobj IFNγ,of
R9175 T11061 T11060 punct ",",IFNγ
R9176 T11062 T11060 conj IL4,IFNγ
R9177 T11063 T11062 punct ",",IL4
R9178 T11064 T11062 conj IL2,IL4
R9179 T11065 T11064 punct ",",IL2
R9180 T11066 T11064 cc and,IL2
R9181 T11067 T11064 conj IL17A,IL2
R9182 T11068 T11058 acl measured,secretion
R9183 T11069 T11068 agent by,measured
R9184 T11070 T11069 pobj ELISA,by
R9185 T11071 T11055 punct .,collected
R9186 T11072 T11073 amod Negative,controls
R9187 T11073 T11074 nsubj controls,are
R9188 T11074 T11098 ccomp are,stimulated
R9189 T11075 T11074 attr 1,are
R9190 T11076 T11075 punct ),1
R9191 T11077 T11074 advcl stimulated,are
R9192 T11078 T11077 dobj BMDC,stimulated
R9193 T11079 T11077 prep without,stimulated
R9194 T11080 T11079 pobj co-culture,without
R9195 T11081 T11077 prep with,stimulated
R9196 T11082 T11086 amod allogeneic,cells
R9197 T11083 T11086 compound CD4,cells
R9198 T11084 T11086 nummod +,cells
R9199 T11085 T11086 compound T,cells
R9200 T11086 T11081 pobj cells,with
R9201 T11087 T11098 punct ;,stimulated
R9202 T11088 T11094 meta 2,cells
R9203 T11089 T11088 punct ),2
R9204 T11090 T11094 amod allogeneic,cells
R9205 T11091 T11094 compound CD4,cells
R9206 T11092 T11094 nummod +,cells
R9207 T11093 T11094 compound T,cells
R9208 T11094 T11098 nsubj cells,stimulated
R9209 T11095 T11094 prep without,cells
R9210 T11096 T11095 pobj co-culture,without
R9211 T11097 T11094 prep with,cells
R9212 T11098 T11098 ROOT stimulated,stimulated
R9213 T11099 T11098 dobj BMDC,stimulated
R9214 T11100 T11098 punct .,stimulated
R9215 T11101 T11103 det Both,controls
R9216 T11102 T11103 amod negative,controls
R9464 T11426 T11422 conj mRNA,Gmcsf
R9465 T11427 T11428 auxpass was,determined
R9466 T11428 T11428 ROOT determined,determined
R9467 T11429 T11428 agent by,determined
R9468 T11430 T11429 pobj qRT-PCR,by
R9469 T11431 T11428 punct .,determined
R9470 T11432 T11436 nsubjpass Primers,purchased
R9471 T11433 T11432 cc and,Primers
R9472 T11434 T11432 conj probes,Primers
R9473 T11435 T11436 auxpass were,purchased
R9474 T11436 T11436 ROOT purchased,purchased
R9475 T11437 T11436 prep from,purchased
R9476 T11438 T11439 compound Applied,Biosystems
R9477 T11439 T11437 pobj Biosystems,from
R9478 T11440 T11436 punct .,purchased
R9479 T11441 T11442 amod Reverse,transcription
R9480 T11442 T11444 nsubjpass transcription,performed
R9481 T11443 T11444 auxpass was,performed
R9482 T11444 T11444 ROOT performed,performed
R9483 T11445 T11444 prep at,performed
R9484 T11446 T11447 nummod 42,°
R9485 T11447 T11445 pobj °,at
R9486 T11448 T11444 npadvmod C,performed
R9487 T11449 T11444 prep for,performed
R9488 T11450 T11451 nummod 60,minutes
R9489 T11451 T11449 pobj minutes,for
R9490 T11452 T11451 prep with,minutes
R9491 T11453 T11456 det the,step
R9492 T11454 T11456 amod final,step
R9493 T11455 T11456 compound denaturation,step
R9494 T11456 T11452 pobj step,with
R9495 T11457 T11456 prep at,step
R9496 T11458 T11459 nummod 90,°
R9497 T11459 T11457 pobj °,at
R9498 T11460 T11461 compound C,for
R9499 T11461 T11444 prep for,performed
R9500 T11462 T11463 nummod 5,minutes
R9501 T11463 T11461 pobj minutes,for
R9502 T11464 T11463 prep in,minutes
R9503 T11465 T11466 nummod 30,µl
R9504 T11466 T11464 pobj µl,in
R9505 T11467 T11466 acl containing,µl
R9506 T11468 T11469 nummod 0.5,µg
R9507 T11469 T11467 dobj µg,containing
R9508 T11470 T11469 prep of,µg
R9509 T11471 T11472 amod total,RNA
R9510 T11472 T11470 pobj RNA,of
R9511 T11473 T11444 punct .,performed
R9512 T11474 T11502 nsubjpass dNTPs,used
R9513 T11475 T11474 punct ",",dNTPs
R9514 T11476 T11474 conj oligo,dNTPs
R9515 T11477 T11478 punct (,dT
R9516 T11478 T11481 nmod dT,primer
R9517 T11479 T11478 punct ),dT
R9518 T11480 T11481 nummod 15,primer
R9519 T11481 T11474 appos primer,dNTPs
R9520 T11482 T11481 punct ",",primer
R9521 T11483 T11486 amod recombinant,Inhibitor
R9522 T11484 T11486 compound RNasin,Inhibitor
R9523 T11485 T11486 compound Ribonuclease,Inhibitor
R9524 T11486 T11481 appos Inhibitor,primer
R9525 T11487 T11481 punct ",",primer
R9526 T11488 T11481 cc and,primer
R9527 T11489 T11490 nsubj M-MLV,Reverse
R9528 T11490 T11502 advmod Reverse,used
R9529 T11491 T11490 npadvmod Transcriptase,Reverse
R9530 T11492 T11493 punct (,all
R9544 T11506 T11508 poss manufacturer,instruction
R9545 T11507 T11506 case 's,manufacturer
R9546 T11508 T11504 pobj instruction,to
R9547 T11509 T11502 punct .,used
R9548 T11510 T11511 amod Reverse,transcription
R9549 T11511 T11513 nsubjpass transcription,done
R9550 T11512 T11513 auxpass was,done
R9551 T11513 T11513 ROOT done,done
R9552 T11514 T11513 prep in,done
R9553 T11515 T11519 det a,Cycler
R9554 T11516 T11519 compound PTC-200,Cycler
R9555 T11517 T11519 compound Peltier,Cycler
R9556 T11518 T11519 compound Thermal,Cycler
R9557 T11519 T11514 pobj Cycler,in
R9558 T11520 T11522 punct (,Research
R9559 T11521 T11522 compound MJ,Research
R9560 T11522 T11519 appos Research,Cycler
R9561 T11523 T11522 punct ",",Research
R9562 T11524 T11522 npadvmod Watertown,Research
R9563 T11525 T11524 punct ",",Watertown
R9564 T11526 T11522 appos MA,Research
R9565 T11527 T11522 punct ),Research
R9566 T11528 T11513 punct .,done
R9567 T11529 T11531 nsubjpass qRT-PCR,performed
R9568 T11530 T11531 auxpass was,performed
R9569 T11531 T11531 ROOT performed,performed
R9570 T11532 T11531 prep in,performed
R9571 T11533 T11534 compound ABI,Prism
R9572 T11534 T11538 compound Prism,System
R9573 T11535 T11538 nummod 7700,System
R9574 T11536 T11538 compound Sequence,System
R9575 T11537 T11538 compound Detection,System
R9576 T11538 T11532 pobj System,in
R9577 T11539 T11538 prep with,System
R9578 T11540 T11541 compound TaqMan,®
R9579 T11541 T11539 pobj ®,with
R9580 T11542 T11544 compound Gene,Assays
R9581 T11543 T11544 compound Expression,Assays
R9582 T11544 T11538 conj Assays,System
R9583 T11545 T11547 punct (,Biosystems
R9584 T11546 T11547 compound Applied,Biosystems
R9585 T11547 T11544 parataxis Biosystems,Assays
R9586 T11548 T11547 punct ",",Biosystems
R9587 T11549 T11550 compound Foster,City
R9588 T11550 T11547 conj City,Biosystems
R9589 T11551 T11550 punct ",",City
R9590 T11552 T11550 npadvmod CA,City
R9591 T11553 T11531 punct ),performed
R9592 T11554 T11531 prep for,performed
R9593 T11555 T11556 compound mouse,Actb
R9594 T11556 T11554 pobj Actb,for
R9595 T11557 T11556 punct ",",Actb
R9596 T11558 T11556 conj IL6,Actb
R9597 T11559 T11558 punct ",",IL6
R9598 T11560 T11558 conj IL12p40,IL6
R9599 T11561 T11560 punct ",",IL12p40
R9600 T11562 T11560 conj Ifnβ,IL12p40
R9601 T11563 T11562 punct ",",Ifnβ
R9602 T11564 T11562 conj Gmcsf,Ifnβ
R9603 T11565 T11564 punct ",",Gmcsf
R9605 T11567 T11564 conj Sharpin,Gmcsf
R9606 T11568 T11531 prep according,performed
R9607 T11569 T11568 prep to,according
R9608 T11570 T11571 det the,manufacturer
R9609 T11571 T11573 poss manufacturer,protocol
R9610 T11572 T11571 case 's,manufacturer
R9611 T11573 T11569 pobj protocol,to
R9612 T11574 T11531 punct .,performed
R9613 T11575 T11577 det The,standard
R9614 T11576 T11577 amod endogenous,standard
R10033 T12090 T12091 compound Immunofluorescence,Fibroblast
R10034 T12091 T12096 nsubjpass Fibroblast,transfected
R10035 T12092 T12091 cc and,Fibroblast
R10036 T12093 T12094 amod macrophage,cells
R10037 T12094 T12091 conj cells,Fibroblast
R10038 T12095 T12096 auxpass were,transfected
R10039 T12096 T12096 ROOT transfected,transfected
R10040 T12097 T12096 prep with,transfected
R10041 T12098 T12097 pobj pFLAG-SHARPIN,with
R10042 T12099 T12096 punct .,transfected
R10043 T12100 T12101 nummod 48,hours
R10044 T12101 T12102 npadvmod hours,later
R10045 T12102 T12106 advmod later,washed
R10046 T12103 T12106 punct ",",washed
R10047 T12104 T12106 nsubjpass cells,washed
R10048 T12105 T12106 auxpass were,washed
R10049 T12106 T12106 ROOT washed,washed
R10050 T12107 T12106 advmod briefly,washed
R10051 T12108 T12106 prep with,washed
R10052 T12109 T12108 pobj PBS,with
R10053 T12110 T12106 punct ",",washed
R10054 T12111 T12112 advmod then,fixed
R10055 T12112 T12106 conj fixed,washed
R10056 T12113 T12112 prep in,fixed
R10057 T12114 T12115 amod cold,methanol
R10058 T12115 T12113 pobj methanol,in
R10059 T12116 T12112 prep at,fixed
R10060 T12117 T12127 preconj −,at
R10061 T12118 T12119 nummod 20,°
R10062 T12119 T12120 compound °,C
R10063 T12120 T12117 appos C,−
R10064 T12121 T12120 prep for,C
R10140 T12197 T12198 compound room,temperature
R10141 T12198 T12196 pobj temperature,at
R10142 T12199 T12182 prep for,incubated
R10143 T12200 T12201 nummod 30,minutes
R10144 T12201 T12199 pobj minutes,for
R10145 T12202 T12182 punct ",",incubated
R10146 T12203 T12182 advcl followed,incubated
R10147 T12204 T12203 agent by,followed
R10148 T12205 T12207 nummod three,washes
R10149 T12206 T12207 amod 5-minute,washes
R10150 T12207 T12204 pobj washes,by
R10151 T12208 T12182 punct .,incubated
R10152 T12209 T12212 nsubjpass Cells,examined
R10153 T12210 T12212 auxpass were,examined
R10154 T12211 T12212 advmod then,examined
R10155 T12212 T12212 ROOT examined,examined
R10156 T12213 T12212 advcl using,examined
R10157 T12214 T12216 amod inverse,microscope
R10158 T12215 T12216 compound fluorescence,microscope
R10159 T12216 T12213 dobj microscope,using
R10160 T12217 T12212 punct .,examined
R10273 T12386 T12407 nsubj Immunoblots,collected
R10274 T12387 T12407 prep After,collected
R10275 T12388 T12387 pcomp adding,After
R10276 T12389 T12390 amod stimulating,ligands
R10277 T12390 T12388 dobj ligands,adding
R10278 T12391 T12390 punct ",",ligands
R10279 T12392 T12394 nummod 100,LPS
R10280 T12393 T12394 compound ng/mL,LPS
R10281 T12394 T12390 conj LPS,ligands
R10282 T12395 T12394 cc or,LPS
R10283 T12396 T12398 quantmod 25,/
R10284 T12397 T12398 quantmod µg,/
R10285 T12398 T12400 npadvmod /,poly
R10286 T12399 T12400 compound mL,poly
R10287 T12400 T12394 conj poly,LPS
R10288 T12401 T12394 conj I,LPS
R10289 T12402 T12394 punct :,LPS
R10290 T12403 T12394 conj C,LPS
R10291 T12404 T12407 punct ",",collected
R10292 T12405 T12407 nsubjpass BMDC,collected
R10293 T12406 T12407 auxpass were,collected
R10294 T12407 T12407 ROOT collected,collected
R10295 T12408 T12407 cc and,collected
R10296 T12409 T12407 conj lysed,collected
R9531 T11493 T11494 advmod all,from
R9532 T11494 T11490 prep from,Reverse
R9533 T11495 T11494 pobj Promega,from
R9534 T11496 T11495 punct ",",Promega
R9535 T11497 T11495 conj Madison,Promega
R9536 T11498 T11497 punct ",",Madison
R9537 T11499 T11497 conj WI,Madison
R9538 T11500 T11490 punct ),Reverse
R9615 T11577 T11584 nsubj standard,was
R9616 T11578 T11577 prep for,standard
R9617 T11579 T11578 pobj normalization,for
R9618 T11580 T11579 prep of,normalization
R9619 T11581 T11583 det the,gene
R9620 T11582 T11583 compound target,gene
R9621 T11583 T11580 pobj gene,of
R9622 T11584 T11584 ROOT was,was
R9623 T11585 T11584 acomp β-actin,was
R9624 T11586 T11584 punct .,was
R9625 T11587 T11589 amod Relative,expression
R9626 T11588 T11589 compound gene,expression
R9627 T11589 T11591 nsubjpass expression,calculated
R9628 T11590 T11591 auxpass was,calculated
R9629 T11591 T11591 ROOT calculated,calculated
R9630 T11592 T11591 advcl using,calculated
R9631 T11593 T11597 det the,method
R9632 T11594 T11595 nummod 2,−
R9633 T11595 T11597 compound −,method
R9634 T11596 T11597 compound ΔΔCt,method
R9635 T11597 T11592 dobj method,using
R9636 T11598 T11600 nmod [,]
R9637 T11599 T11600 nummod 56,]
R9638 T11600 T11597 appos ],method
R9639 T11601 T11591 punct .,calculated
R9884 T11925 T11934 prep In,cultured
R9885 T11926 T11928 amod vitro,secretion
R9886 T11927 T11928 compound cytokine,secretion
R9887 T11928 T11925 pobj secretion,In
R9888 T11929 T11928 prep by,secretion
R9889 T11930 T11929 pobj BMDC,by
R9890 T11931 T11932 det The,BMDC
R9891 T11932 T11934 nsubjpass BMDC,cultured
R9892 T11933 T11934 auxpass were,cultured
R9893 T11934 T11934 ROOT cultured,cultured
R9894 T11935 T11934 prep in,cultured
R9895 T11936 T11937 amod triplicate,wells
R9896 T11937 T11935 pobj wells,in
R9897 T11938 T11937 prep of,wells
R9898 T11939 T11942 amod 24-well,plates
R9899 T11940 T11939 cc or,24-well
R9900 T11941 T11939 conj 96-well,24-well
R9901 T11942 T11938 pobj plates,of
R9902 T11943 T11934 prep at,cultured
R9903 T11944 T11947 nummod 106,cells/mL
R9904 T11945 T11944 cc or,106
R9905 T11946 T11944 conj 105,106
R9906 T11947 T11943 pobj cells/mL,at
R9907 T11948 T11947 punct ",",cells/mL
R9908 T11949 T11947 npadvmod respectively,cells/mL
R9909 T11950 T11947 punct ",",cells/mL
R9910 T11951 T11947 prep in,cells/mL
R9911 T11952 T11954 amod RPMI-1640,medium
R9912 T11953 T11954 amod complete,medium
R9913 T11954 T11951 pobj medium,in
R9914 T11955 T11934 punct .,cultured
R9915 T11956 T11957 det The,cells
R9916 T11957 T11959 nsubjpass cells,washed
R9917 T11958 T11959 auxpass were,washed
R9918 T11959 T11959 ROOT washed,washed
R9919 T11960 T11959 cc and,washed
R9920 T11961 T11959 conj stimulated,washed
R9921 T11962 T11961 prep with,stimulated
R9922 T11963 T11965 nummod 100,LPS
R9923 T11964 T11965 compound ng/mL,LPS
R9924 T11965 T11962 pobj LPS,with
R9925 T11966 T11965 cc or,LPS
R9926 T11967 T11969 quantmod 25,/
R9927 T11968 T11969 quantmod µg,/
R9928 T11969 T11972 npadvmod /,I
R9929 T11970 T11971 compound mL,poly
R9930 T11971 T11972 dep poly,I
R9931 T11972 T11965 conj I,LPS
R9932 T11973 T11972 punct :,I
R9933 T11974 T11972 appos C,I
R9934 T11975 T11959 punct .,washed
R9935 T11976 T11982 prep After,harvested
R9936 T11977 T11978 nummod 24,hours
R9937 T11978 T11976 pobj hours,After
R9938 T11979 T11982 punct ",",harvested
R9939 T11980 T11982 nsubjpass supernatants,harvested
R9940 T11981 T11982 auxpass were,harvested
R9941 T11982 T11982 ROOT harvested,harvested
R9942 T11983 T11982 prep for,harvested
R9943 T11984 T11985 compound ELISA,analysis
R9944 T11985 T11983 pobj analysis,for
R9945 T11986 T11982 punct .,harvested
R9946 T11987 T11988 det The,presence
R9947 T11988 T11995 nsubjpass presence,determined
R9948 T11989 T11988 prep of,presence
R9949 T11990 T11989 pobj nitrite,of
R9950 T11991 T11988 prep in,presence
R9951 T11992 T11993 det the,supernatants
R9952 T11993 T11991 pobj supernatants,in
R9953 T11994 T11995 auxpass was,determined
R9954 T11995 T11995 ROOT determined,determined
R9955 T11996 T11995 advcl using,determined
R9956 T11997 T11999 det the,reagent
R9957 T11998 T11999 compound Griess,reagent
R9958 T11999 T11996 dobj reagent,using
R9959 T12000 T11995 punct .,determined
R10065 T12122 T12123 nummod 10,minutes
R10066 T12123 T12121 pobj minutes,for
R10067 T12124 T12123 cc and,minutes
R10068 T12125 T12126 amod cold,acetone
R10069 T12126 T12123 conj acetone,minutes
R10070 T12127 T12112 prep at,fixed
R10071 T12128 T12129 nummod -20,°
R10072 T12129 T12130 nummod °,C
R10073 T12130 T12127 pobj C,at
R10074 T12131 T12112 prep for,fixed
R10075 T12132 T12133 nummod 1,minute
R10076 T12133 T12131 pobj minute,for
R10077 T12134 T12106 punct .,washed
R10078 T12135 T12136 compound Incubate,cells
R10079 T12136 T12136 ROOT cells,cells
R10080 T12137 T12136 prep with,cells
R10081 T12138 T12137 pobj PBS,with
R10082 T12139 T12140 nummod +1,%
R10083 T12140 T12141 compound %,BSA
R10084 T12141 T12136 appos BSA,cells
R10085 T12142 T12141 prep for,BSA
R10086 T12143 T12144 nummod 15,minutes
R10087 T12144 T12142 pobj minutes,for
R10088 T12145 T12146 aux to,block
R10089 T12146 T12136 xcomp block,cells
R10090 T12147 T12148 amod non-specific,binding
R10091 T12148 T12146 dobj binding,block
R10092 T12149 T12136 punct .,cells
R10093 T12150 T12151 amod Fixed,cells
R10094 T12151 T12154 nsubjpass cells,incubated
R10095 T12152 T12154 auxpass were,incubated
R10096 T12153 T12154 advmod then,incubated
R10097 T12154 T12154 ROOT incubated,incubated
R10098 T12155 T12154 prep with,incubated
R10099 T12156 T12155 pobj anti-FLAG,with
R10100 T12157 T12160 punct (,100
R10101 T12158 T12160 nummod 1,100
R10102 T12159 T12160 nummod ∶,100
R10103 T12160 T12156 appos 100,anti-FLAG
R10104 T12161 T12160 punct ),100
R10105 T12162 T12156 prep as,anti-FLAG
R10106 T12163 T12165 det the,antibody
R10107 T12164 T12165 amod primary,antibody
R10108 T12165 T12162 pobj antibody,as
R10109 T12166 T12165 prep at,antibody
R10110 T12167 T12168 compound room,temperature
R10111 T12168 T12166 pobj temperature,at
R10112 T12169 T12154 prep for,incubated
R10297 T12410 T12409 prep at,lysed
R10298 T12411 T12410 pobj 0,at
R10299 T12412 T12409 punct -,lysed
R10300 T12413 T12409 punct ",",lysed
R10301 T12414 T12423 nummod 15,points
R10302 T12415 T12423 punct -,points
R10303 T12416 T12423 punct ",",points
R10304 T12417 T12423 nummod 30,points
R10305 T12418 T12417 punct -,30
R10306 T12419 T12418 punct ",",-
R10307 T12420 T12417 cc and,30
R10308 T12421 T12423 amod 60-minute,points
R10309 T12422 T12423 compound time,points
R10310 T12423 T12407 npadvmod points,collected
R10311 T12424 T12407 punct .,collected
R10312 T12425 T12427 nsubjpass Immunoblots,performed
R10313 T12426 T12427 auxpass were,performed
R10314 T12427 T12427 ROOT performed,performed
R10315 T12428 T12427 prep with,performed
R10316 T12429 T12428 pobj antibodies,with
R10317 T12430 T12433 punct (,Technology
R10318 T12431 T12433 compound Cell,Technology
R10319 T12432 T12433 compound Signaling,Technology
R10320 T12433 T12429 appos Technology,antibodies
R10321 T12434 T12429 punct ),antibodies
R10322 T12435 T12427 prep against,performed
R10323 T12436 T12435 pobj p-IKK1,against
R10324 T12437 T12427 advcl /,performed
R10325 T12438 T12437 nummod 2,/
R10326 T12439 T12440 punct (,#
R10327 T12440 T12441 nmod #,2697
R10328 T12441 T12437 appos 2697,/
R10329 T12442 T12437 punct ),/
R10330 T12443 T12437 punct ",",/
R10331 T12444 T12444 ROOT p-IκBα,p-IκBα
R10332 T12445 T12446 punct (,#
R10333 T12446 T12446 ROOT #,#
R10334 T12447 T12446 npadvmod 9246,#
R10335 T12448 T12446 punct ),#
R10336 T12449 T12446 punct ",",#
R10337 T12450 T12446 conj IκBα,#
R10338 T12451 T12452 punct (,#
R10339 T12452 T12453 nmod #,4814
R10340 T12453 T12450 parataxis 4814,IκBα
R10341 T12454 T12453 punct ),4814
R10342 T12455 T12450 punct ",",IκBα
R10343 T12456 T12450 conj p-TBK1,IκBα
R10344 T12457 T12456 punct (,p-TBK1
R10345 T12458 T12459 nmod #,5483
R10346 T12459 T12456 appos 5483,p-TBK1
R10347 T12460 T12456 punct ),p-TBK1
R10348 T12461 T12456 punct ",",p-TBK1
R10349 T12462 T12456 conj p-p38,p-TBK1
R10350 T12463 T12464 punct (,#
R10351 T12464 T12462 parataxis #,p-p38
R10352 T12465 T12464 dep 9216,#
R10353 T12466 T12464 punct ),#
R10354 T12467 T12462 punct ",",p-p38
R10355 T12468 T12462 appos p38,p-p38
R10356 T12469 T12471 punct (,9212
R10357 T12470 T12471 nmod #,9212
R10358 T12471 T12468 parataxis 9212,p38
R10360 T12473 T12462 punct ",",p-p38
R10361 T12474 T12475 compound p-ERK1,/
R10362 T12475 T12456 appos /,p-TBK1
R10363 T12476 T12475 nummod 2,/
R10364 T12477 T12478 punct (,#
R10365 T12478 T12475 appos #,/
R10366 T12479 T12478 nummod 4376,#
R10367 T12480 T12478 punct ),#
R10368 T12481 T12475 punct ",",/
R10369 T12482 T12475 cc and,/
R10370 T12483 T12475 conj ERK1/2,/
R10371 T12484 T12485 punct (,#
R10372 T12485 T12483 parataxis #,ERK1/2
R10373 T12486 T12485 npadvmod 4695,#
R10374 T12487 T12485 punct ),#
R10606 T12819 T12818 prep in,patterns
R10607 T12820 T12822 det the,protein
R10608 T12821 T12822 compound SHARPIN,protein
R10609 T12822 T12819 pobj protein,in
R10610 T12823 T12822 prep of,protein
R10611 T12824 T12826 amod human,mouse
R10612 T12825 T12824 punct ",",human
R10613 T12826 T12823 pobj mouse,of
R10614 T12827 T12826 cc and,mouse
R10615 T12828 T12829 compound rat,origins
R10616 T12829 T12826 conj origins,mouse
R10617 T12830 T12788 punct .,used
R10618 T12831 T12832 punct (,B
R10619 T12832 T12832 ROOT B,B
R10620 T12833 T12832 punct ),B
R10621 T12834 T12836 nummod Eight,females
R10622 T12835 T12836 amod week-old,females
R10623 T12836 T12836 ROOT females,females
R10624 T12837 T12836 prep of,females
R10625 T12838 T12841 nmod WT,mice
R10626 T12839 T12838 cc and,WT
R10627 T12840 T12841 compound cpdm,mice
R10628 T12841 T12837 pobj mice,of
R10629 T12842 T12836 punct .,females
R10630 T12843 T12845 det The,mice
R10631 T12844 T12845 amod mutant,mice
R10632 T12845 T12849 nsubj mice,develop
R10633 T12846 T12847 punct (,above
R10634 T12847 T12845 advmod above,mice
R10635 T12848 T12847 punct ),above
R10636 T12849 T12849 ROOT develop,develop
R10637 T12850 T12852 amod progressive,inflammation
R10638 T12851 T12852 compound skin,inflammation
R10639 T12852 T12849 dobj inflammation,develop
R10640 T12853 T12849 advcl starting,develop
R10641 T12854 T12853 prep at,starting
R10642 T12855 T12856 advmod about,four
R10643 T12856 T12857 nummod four,weeks
R10644 T12857 T12854 pobj weeks,at
R10645 T12858 T12849 punct .,develop
R10646 T12859 T12860 punct (,C
R10647 T12860 T12860 ROOT C,C
R10648 T12861 T12860 punct ),C
R10649 T12862 T12863 amod Extramedullary,hematopoiesis
R10650 T12863 T12864 nsubj hematopoiesis,causes
R10651 T12864 T12864 ROOT causes,causes
R10652 T12865 T12866 amod marked,enlargement
R10653 T12866 T12864 dobj enlargement,causes
R10654 T12867 T12866 prep of,enlargement
R10655 T12868 T12869 det the,spleen
R10656 T12869 T12867 pobj spleen,of
R10657 T12870 T12869 prep of,spleen
R10658 T12871 T12872 compound cpdm,mice
R10659 T12872 T12870 pobj mice,of
R10660 T12873 T12864 punct .,causes
R10661 T12874 T12877 punct (,BMDC
R10662 T12875 T12877 nmod D,BMDC
R10663 T12876 T12877 punct ),BMDC
R10664 T12877 T12879 nsubjpass BMDC,incubated
R10665 T12878 T12879 auxpass were,incubated
R10666 T12879 T12879 ROOT incubated,incubated
R10667 T12880 T12879 prep in,incubated
R10668 T12881 T12882 det the,absence
R10669 T12882 T12880 pobj absence,in
R10670 T12883 T12882 cc or,absence
R10671 T12884 T12882 conj presence,absence
R10672 T12885 T12882 prep of,absence
R10673 T12886 T12888 nummod 100,LPS
R10674 T12887 T12888 compound ng/ml,LPS
R10675 T12888 T12885 pobj LPS,of
R10676 T12889 T12879 prep for,incubated
R10113 T12170 T12171 nummod 1,hour
R10114 T12171 T12169 pobj hour,for
R10115 T12172 T12171 punct ",",hour
R10116 T12173 T12154 advcl followed,incubated
R10117 T12174 T12173 agent by,followed
R10118 T12175 T12177 nummod three,washes
R10119 T12176 T12177 amod 5-minute,washes
R10120 T12177 T12174 pobj washes,by
R10121 T12178 T12154 punct .,incubated
R10122 T12179 T12182 nsubjpass Cells,incubated
R10123 T12180 T12182 auxpass were,incubated
R10124 T12181 T12182 advmod further,incubated
R10125 T12182 T12182 ROOT incubated,incubated
R10126 T12183 T12182 prep with,incubated
R10127 T12184 T12186 amod goat,IgG-FITC
R10128 T12185 T12186 amod anti-rabbit,IgG-FITC
R10129 T12186 T12183 pobj IgG-FITC,with
R10130 T12187 T12188 punct (,1
R10131 T12188 T12190 nummod 1,100
R10132 T12189 T12190 nummod ∶,100
R10133 T12190 T12186 appos 100,IgG-FITC
R10134 T12191 T12186 punct ),IgG-FITC
R10135 T12192 T12182 prep as,incubated
R10136 T12193 T12195 det the,antibody
R10137 T12194 T12195 amod secondary,antibody
R10138 T12195 T12192 pobj antibody,as
R10139 T12196 T12195 prep at,antibody
R10375 T12488 T12446 punct .,#
R10376 T12489 T12498 nsubjpass Beta-actin,used
R10377 T12490 T12495 punct (,Biotechnology
R10378 T12491 T12495 amod sc-47778,Biotechnology
R10379 T12492 T12495 punct ",",Biotechnology
R10380 T12493 T12494 compound Santa,Cruz
R10381 T12494 T12495 compound Cruz,Biotechnology
R10382 T12495 T12495 ROOT Biotechnology,Biotechnology
R10383 T12496 T12495 punct ),Biotechnology
R10384 T12497 T12498 auxpass was,used
R10385 T12498 T12498 ROOT used,used
R10386 T12499 T12498 prep as,used
R10387 T12500 T12499 pcomp loading,as
R10388 T12501 T12500 dobj control,loading
R10389 T12502 T12498 punct .,used
R10498 T12682 T12683 compound Statistical,analysis
R10499 T12683 T12684 compound analysis,Data
R10500 T12684 T12686 nsubjpass Data,expressed
R10501 T12685 T12686 auxpass are,expressed
R10502 T12686 T12686 ROOT expressed,expressed
R10503 T12687 T12686 prep as,expressed
R10504 T12688 T12690 amod mean,SD
R10505 T12689 T12690 compound ±,SD
R10506 T12690 T12687 pobj SD,as
R10507 T12691 T12686 punct .,expressed
R10508 T12692 T12694 det The,significance
R10509 T12693 T12694 amod statistical,significance
R10510 T12694 T12703 nsubjpass significance,determined
R10511 T12695 T12694 prep of,significance
R10512 T12696 T12695 pobj differences,of
R10513 T12697 T12696 prep of,differences
R10514 T12698 T12697 pobj means,of
R10515 T12699 T12698 prep between,means
R10516 T12700 T12701 amod experimental,groups
R10517 T12701 T12699 pobj groups,between
R10518 T12702 T12703 auxpass was,determined
R10519 T12703 T12703 ROOT determined,determined
R10520 T12704 T12703 agent by,determined
R10521 T12705 T12707 poss Students,t-test
R10522 T12706 T12705 case ',Students
R10523 T12707 T12704 pobj t-test,by
R10524 T12708 T12703 punct .,determined
R10558 T12771 T12771 ROOT In,In
R10559 T12772 T12771 pobj vivo,In
R10560 T12773 T12771 cc and,In
R10561 T12774 T12771 conj in,In
R10562 T12775 T12776 amod vitro,features
R10563 T12776 T12774 pobj features,in
R10564 T12777 T12776 prep of,features
R10565 T12778 T12777 pobj SHARPIN,of
R10566 T12779 T12771 punct .,In
R10567 T12780 T12788 punct (,used
R10568 T12781 T12786 nmod A,programs
R10569 T12782 T12781 punct ),A
R10570 T12783 T12786 nmod COILS,programs
R10571 T12784 T12783 cc and,COILS
R10572 T12785 T12783 conj MotifScan,COILS
R10573 T12786 T12788 nsubjpass programs,used
R10574 T12787 T12788 auxpass were,used
R10575 T12788 T12788 ROOT used,used
R10576 T12789 T12790 aux to,predict
R10577 T12790 T12788 xcomp predict,used
R10578 T12791 T12792 det the,presence
R10579 T12792 T12790 dobj presence,predict
R10580 T12793 T12792 prep of,presence
R10581 T12794 T12793 pobj CC,of
R10582 T12795 T12794 punct (,CC
R10583 T12796 T12798 nmod coiled-coil,domain
R10584 T12797 T12798 punct ),domain
R10585 T12798 T12794 appos domain,CC
R10586 T12799 T12794 punct ",",CC
R10587 T12800 T12794 conj UBL,CC
R10588 T12801 T12802 punct (,ubiquitin-like
R10589 T12802 T12800 appos ubiquitin-like,UBL
R10590 T12803 T12802 punct ),ubiquitin-like
R10591 T12804 T12793 pobj domain,of
R10592 T12805 T12804 cc and,domain
R10593 T12806 T12813 compound ZFRBP,domain
R10594 T12807 T12810 punct (,protein
R10595 T12808 T12809 compound zinc-finger,Ran-Binding
R10596 T12809 T12810 compound Ran-Binding,protein
R10597 T12810 T12806 appos protein,ZFRBP
R10598 T12811 T12810 nummod 2,protein
R10599 T12812 T12810 punct ),protein
R10600 T12813 T12792 appos domain,presence
R10601 T12814 T12815 nsubj which,form
R10602 T12815 T12813 relcl form,domain
R10603 T12816 T12818 amod similar,patterns
R10604 T12817 T12818 compound motif,patterns
R10605 T12818 T12815 dobj patterns,form
R10681 T12894 T12895 compound mRNA,level
R10682 T12895 T12899 nsubjpass level,measured
R10683 T12896 T12895 prep of,level
R10684 T12897 T12896 pobj Sharpin,of
R10685 T12898 T12899 auxpass was,measured
R10686 T12899 T12899 ROOT measured,measured
R10687 T12900 T12899 agent by,measured
R10688 T12901 T12900 pobj qRT-PCR,by
R10689 T12902 T12899 cc and,measured
R10690 T12903 T12899 conj presented,measured
R10691 T12904 T12903 advmod relative,presented
R10692 T12905 T12904 prep to,relative
R10693 T12906 T12908 det the,expression
R10694 T12907 T12908 compound mRNA,expression
R10695 T12908 T12905 pobj expression,to
R10696 T12909 T12908 prep in,expression
R10697 T12910 T12912 compound non-stimulated,BMDC
R10698 T12911 T12912 compound WT,BMDC
R10699 T12912 T12909 pobj BMDC,in
R10700 T12913 T12899 punct .,measured
R10701 T12914 T12915 nsubj Bars,represent
R10702 T12915 T12915 ROOT represent,represent
R10703 T12916 T12919 det the,s.d.
R10704 T12917 T12919 amod mean,s.d.
R10705 T12918 T12919 compound ±,s.d.
R10706 T12919 T12915 dobj s.d.,represent
R10707 T12920 T12919 prep of,s.d.
R10708 T12921 T12922 nummod 3,mice
R10709 T12922 T12920 pobj mice,of
R10710 T12923 T12915 punct .,represent
R10711 T12924 T12930 punct *,P
R10712 T12925 T12927 compound P,0.05
R10713 T12926 T12927 compound <,0.05
R10714 T12927 T12924 npadvmod 0.05,*
R10715 T12928 T12930 punct ;,P
R10716 T12929 T12930 punct **,P
R10717 T12930 T12930 ROOT P,P
R10718 T12931 T12930 prep <,P
R10719 T12932 T12931 pobj 0.001,<
R10720 T12933 T12930 punct .,P
R10677 T12890 T12891 nummod 4,hours
R10678 T12891 T12889 pobj hours,for
R10679 T12892 T12879 punct .,incubated
R10680 T12893 T12895 det The,level
R10756 T12969 T12970 amod secondary,antibody
R10757 T12970 T12965 pobj antibody,with
R10758 T12971 T12962 punct .,fixed
R10759 T12972 T12974 nsubjpass Nuclei,stained
R10760 T12973 T12974 auxpass were,stained
R10761 T12974 T12974 ROOT stained,stained
R10762 T12975 T12974 prep with,stained
R10763 T12976 T12975 pobj DAPI,with
R10764 T12977 T12974 punct .,stained
R10765 T12978 T12986 prep In,found
R10766 T12979 T12982 det both,lines
R10767 T12980 T12982 amod transfected,lines
R10768 T12981 T12982 compound cell,lines
R10769 T12982 T12978 pobj lines,In
R10770 T12983 T12986 punct ",",found
R10771 T12984 T12986 nsubjpass FLAG-SHARPIN,found
R10772 T12985 T12986 auxpass was,found
R10773 T12986 T12986 ROOT found,found
R10774 T12987 T12988 aux to,be
R10775 T12988 T12986 xcomp be,found
R10776 T12989 T12988 acomp cytoplasm-localized,be
R10777 T12990 T12986 punct .,found
R11007 T13275 T13275 ROOT Effect,Effect
R11008 T13276 T13275 prep of,Effect
R11009 T13277 T13278 compound Sharpin,mutation
R11010 T13278 T13276 pobj mutation,of
R11011 T13279 T13278 prep on,mutation
R11012 T13280 T13281 compound DC,subpopulations
R11013 T13281 T13279 pobj subpopulations,on
R11014 T13282 T13281 cc and,subpopulations
R11015 T13283 T13281 conj maturation,subpopulations
R11016 T13284 T13275 punct .,Effect
R11017 T13285 T13288 punct (,Spleens
R11018 T13286 T13288 nmod A,Spleens
R11019 T13287 T13288 punct ),Spleens
R11020 T13288 T13293 nmod Spleens,mice
R11021 T13289 T13288 prep from,Spleens
R11022 T13290 T13289 pobj WT,from
R11023 T13291 T13290 cc and,WT
R11024 T13292 T13293 compound cpdm,mice
R11025 T13293 T13293 ROOT mice,mice
R11026 T13294 T13293 aux were,mice
R11027 T13295 T13294 acomp isolated,were
R11028 T13296 T13295 cc and,isolated
R11029 T13297 T13295 conj subject,isolated
R11030 T13298 T13297 prep to,subject
R11031 T13299 T13298 pobj collagenase,to
R11032 T13300 T13299 cc and,collagenase
R11033 T13301 T13299 conj DNase,collagenase
R11034 T13302 T13301 dobj digestion,DNase
R11035 T13303 T13294 punct .,were
R11036 T13304 T13307 det The,homogenates
R11037 T13305 T13307 amod obtained,homogenates
R11038 T13306 T13307 amod splenic,homogenates
R11039 T13307 T13309 nsubjpass homogenates,centrifuged
R11040 T13308 T13309 auxpass were,centrifuged
R11041 T13309 T13309 ROOT centrifuged,centrifuged
R11042 T13310 T13309 prep over,centrifuged
R11043 T13311 T13313 det a,gradient
R11044 T13312 T13313 compound Percoll,gradient
R11045 T13313 T13310 pobj gradient,over
R11046 T13314 T13313 punct (,gradient
R11047 T13315 T13316 nummod 35,%
R11048 T13316 T13313 appos %,gradient
R11049 T13317 T13316 cc and,%
R11050 T13318 T13319 nummod 55,%
R11051 T13319 T13320 compound %,density
R11052 T13320 T13316 conj density,%
R11053 T13321 T13313 punct ),gradient
R11054 T13322 T13309 prep for,centrifuged
R11055 T13323 T13324 nummod 15,minutes
R11056 T13324 T13322 pobj minutes,for
R11057 T13325 T13309 punct .,centrifuged
R11058 T13326 T13327 det The,bands
R11059 T13327 T13341 nsubjpass bands,pooled
R11135 T13403 T13411 nsubj gating,gave
R11136 T13404 T13403 prep on,gating
R11137 T13405 T13410 det the,subpopulation
R11138 T13406 T13410 amod CD11c,subpopulation
R11139 T13407 T13410 nmod +,subpopulation
R11140 T13408 T13410 nmod PDCA-1,subpopulation
R11141 T13409 T13410 compound −,subpopulation
R11142 T13410 T13404 pobj subpopulation,on
R11143 T13411 T13411 ROOT gave,gave
R11144 T13412 T13414 det the,panel
R11145 T13413 T13414 amod bottom,panel
R11146 T13414 T13411 dobj panel,gave
R11147 T13415 T13416 nsubj that,showed
R11148 T13416 T13414 relcl showed,panel
R11149 T13417 T13419 nummod two,pools
R11150 T13418 T13419 amod distinct,pools
R11151 T13419 T13416 dobj pools,showed
R11152 T13420 T13419 prep of,pools
R11153 T13421 T13424 amod CD11c,+
R11154 T13422 T13424 compound +,+
R11155 T13423 T13424 compound CD8α,+
R11156 T13424 T13420 pobj +,of
R11157 T13425 T13424 cc and,+
R11158 T13426 T13424 conj CD11c,+
R11159 T13427 T13416 ccomp +,showed
R11160 T13428 T13430 amod CD8α,cells
R11161 T13429 T13430 compound −,cells
R11162 T13430 T13427 dobj cells,+
R11163 T13431 T13411 punct .,gave
R11164 T13432 T13434 nsubjpass Percentages,calculated
R11165 T13433 T13434 auxpass were,calculated
R11166 T13434 T13434 ROOT calculated,calculated
R11167 T13435 T13434 prep based,calculated
R11168 T13436 T13435 prep on,based
R11169 T13437 T13439 det the,population
R11170 T13438 T13439 amod parental,population
R11171 T13439 T13436 pobj population,on
R11172 T13440 T13434 cc and,calculated
R11173 T13441 T13443 auxpass were,shown
R11174 T13442 T13443 advmod additionally,shown
R11175 T13443 T13434 conj shown,calculated
R11176 T13444 T13443 prep as,shown
R11177 T13445 T13446 compound bar,graphs
R11178 T13446 T13444 pobj graphs,as
R11179 T13447 T13446 punct (,graphs
R11180 T13448 T13446 appos n,graphs
R11181 T13449 T13450 punct =,2
R11182 T13450 T13453 meta 2,B
R11183 T13451 T13450 punct ),2
R11184 T13452 T13453 punct (,B
R11185 T13453 T13446 appos B,graphs
R11186 T13454 T13453 punct ),B
R11187 T13455 T13443 punct .,shown
R11188 T13456 T13459 punct (,WT
R11189 T13457 T13459 compound C,WT
R11190 T13458 T13457 punct ),C
R11191 T13459 T13468 nmod WT,cells
R11192 T13460 T13459 cc and,WT
R11193 T13461 T13462 compound cpdm,BMDC
R11194 T13462 T13459 conj BMDC,WT
R11195 T13463 T13464 punct (,5
R11196 T13464 T13468 nummod 5,cells
R11197 T13465 T13466 nummod ×,105
R11198 T13466 T13464 appos 105,5
R11199 T13467 T13464 punct ),5
R11200 T13468 T13470 nsubjpass cells,stimulated
R11201 T13469 T13470 auxpass were,stimulated
R11202 T13470 T13470 ROOT stimulated,stimulated
R11203 T13471 T13470 prep with,stimulated
R11204 T13472 T13471 pobj medium,with
R10721 T12934 T12937 punct (,Fibroblast
R10722 T12935 T12937 nmod E,Fibroblast
R10723 T12936 T12937 punct ),Fibroblast
R10724 T12937 T12947 nmod Fibroblast,cells
R10725 T12938 T12939 punct (,NIH3T3
R10726 T12939 T12937 appos NIH3T3,Fibroblast
R10727 T12940 T12939 punct ),NIH3T3
R10728 T12941 T12937 cc and,Fibroblast
R10729 T12942 T12937 conj macrophages,Fibroblast
R10730 T12943 T12942 punct (,macrophages
R10731 T12944 T12945 nummod RAW264,.7
R10732 T12945 T12942 appos .7,macrophages
R10733 T12946 T12942 punct ),macrophages
R10734 T12947 T12949 nsubjpass cells,transfected
R10735 T12948 T12949 auxpass were,transfected
R10736 T12949 T12949 ROOT transfected,transfected
R10737 T12950 T12949 prep with,transfected
R10738 T12951 T12952 det the,expression
R10739 T12952 T12950 pobj expression,with
R10740 T12953 T12949 conj plasmid,transfected
R10741 T12954 T12953 nsubj pFLAG-SHARPIN,plasmid
R10742 T12955 T12949 punct .,transfected
R10743 T12956 T12962 prep After,fixed
R10744 T12957 T12958 nummod 48,hours
R10745 T12958 T12956 pobj hours,After
R10746 T12959 T12962 punct ",",fixed
R10747 T12960 T12962 nsubjpass cells,fixed
R10748 T12961 T12962 auxpass were,fixed
R10749 T12962 T12962 ROOT fixed,fixed
R10750 T12963 T12962 cc and,fixed
R10751 T12964 T12962 conj probed,fixed
R10752 T12965 T12964 prep with,probed
R10753 T12966 T12970 amod anti-FLAG,antibody
R10754 T12967 T12966 cc and,anti-FLAG
R10755 T12968 T12966 conj FITC-conjugated,anti-FLAG
R11060 T13328 T13327 prep at,bands
R11061 T13329 T13333 det the,medium
R11062 T13330 T13331 nummod 35,%
R11063 T13331 T13333 compound %,medium
R11064 T13332 T13333 punct -,medium
R11065 T13333 T13328 pobj medium,at
R11066 T13334 T13333 cc and,medium
R11067 T13335 T13336 det the,35
R11068 T13336 T13333 conj 35,medium
R11069 T13337 T13338 nummod 55,%
R11070 T13338 T13339 compound %,interface
R11071 T13339 T13341 nsubjpass interface,pooled
R11072 T13340 T13341 auxpass were,pooled
R11073 T13341 T13341 ROOT pooled,pooled
R11074 T13342 T13341 punct ",",pooled
R11075 T13343 T13341 conj washed,pooled
R11076 T13344 T13343 cc and,washed
R11077 T13345 T13343 conj stained,washed
R11078 T13346 T13345 prep with,stained
R11079 T13347 T13348 det a,combination
R11080 T13348 T13346 pobj combination,with
R11081 T13349 T13348 prep of,combination
R11082 T13350 T13351 amod various,antibodies
R11083 T13351 T13349 pobj antibodies,of
R11084 T13352 T13353 aux to,stain
R11085 T13353 T13345 advcl stain,stained
R11086 T13354 T13356 amod different,subsets
R11087 T13355 T13356 compound DC,subsets
R11088 T13356 T13353 dobj subsets,stain
R11089 T13357 T13356 punct ",",subsets
R11090 T13358 T13360 amod conventional,+
R11091 T13359 T13360 amod CD11c,+
R11092 T13360 T13362 compound +,+
R11093 T13361 T13362 compound CD8α,+
R11094 T13362 T13353 npadvmod +,stain
R11095 T13363 T13362 punct ",",+
R11096 T13364 T13367 compound CD11c,−
R11097 T13365 T13367 compound +,−
R11098 T13366 T13367 compound CD8α,−
R11099 T13367 T13362 conj −,+
R11100 T13368 T13367 cc and,−
R11101 T13369 T13370 amod plasmacytoid,DC
R11102 T13370 T13367 conj DC,−
R11103 T13371 T13375 punct (,+
R11104 T13372 T13375 compound CD11c,+
R11105 T13373 T13375 compound −,+
R11106 T13374 T13375 compound PDCA-1,+
R11107 T13375 T13367 appos +,−
R11108 T13376 T13367 punct ),−
R11109 T13377 T13341 punct .,pooled
R11110 T13378 T13380 det The,panels
R11111 T13379 T13380 amod top,panels
R11112 T13380 T13382 nsubjpass panels,gated
R11113 T13381 T13382 auxpass were,gated
R11114 T13382 T13382 ROOT gated,gated
R11115 T13383 T13382 prep on,gated
R11116 T13384 T13385 compound FSChiSSClo,cells
R11117 T13385 T13383 pobj cells,on
R11118 T13386 T13387 aux to,show
R11119 T13387 T13382 advcl show,gated
R11120 T13388 T13389 amod separate,populations
R11121 T13389 T13387 dobj populations,show
R11122 T13390 T13389 prep of,populations
R11123 T13391 T13394 amod CD11c,−
R11124 T13392 T13394 nmod +,−
R11125 T13393 T13394 compound PDCA-1,−
R11126 T13394 T13400 nmod −,cells
R11127 T13395 T13394 cc and,−
R11128 T13396 T13400 amod CD11c,cells
R11129 T13397 T13400 compound −,cells
R11130 T13398 T13400 compound PDCA-1,cells
R11131 T13399 T13400 compound +,cells
R11132 T13400 T13390 pobj cells,of
R11133 T13401 T13382 punct .,gated
R11134 T13402 T13403 amod Further,gating
R11210 T13478 T13482 nummod 25,poly
R11211 T13479 T13482 nmod µg,poly
R11212 T13480 T13482 nmod /,poly
R11213 T13481 T13482 compound ml,poly
R11214 T13482 T13476 conj poly,LPS
R11215 T13483 T13485 nsubj I,C
R11216 T13484 T13485 punct :,C
R11217 T13485 T13470 npadvmod C,stimulated
R11218 T13486 T13485 prep for,C
R11219 T13487 T13488 nummod 24,hours
R11220 T13488 T13486 pobj hours,for
R11221 T13489 T13470 punct .,stimulated
R11222 T13490 T13491 det The,cells
R11223 T13491 T13493 nsubjpass cells,labeled
R11224 T13492 T13493 auxpass were,labeled
R11225 T13493 T13493 ROOT labeled,labeled
R11226 T13494 T13493 prep with,labeled
R11227 T13495 T13504 amod PE-labeled,subjected
R11228 T13496 T13504 amod anti-CD40,subjected
R11229 T13497 T13496 punct ",",anti-CD40
R11230 T13498 T13496 conj anti-CD80,anti-CD40
R11231 T13499 T13498 punct ",",anti-CD80
R11232 T13500 T13498 cc and,anti-CD80
R11233 T13501 T13498 conj anti-CD86,anti-CD80
R11234 T13502 T13501 punct ",",anti-CD86
R11235 T13503 T13498 cc and,anti-CD80
R11236 T13504 T13493 conj subjected,labeled
R11237 T13505 T13506 aux to,flow
R11238 T13506 T13504 xcomp flow,subjected
R11239 T13507 T13508 amod cytometry,analysis
R11240 T13508 T13506 dobj analysis,flow
R11241 T13509 T13493 punct .,labeled
R11242 T13510 T13511 det The,populations
R11243 T13511 T13516 nsubjpass populations,gated
R11244 T13512 T13511 acl shown,populations
R11245 T13513 T13512 prep in,shown
R11246 T13514 T13513 pobj histograms,in
R11247 T13515 T13516 auxpass were,gated
R11248 T13516 T13516 ROOT gated,gated
R11249 T13517 T13516 prep on,gated
R11250 T13518 T13520 amod CD11c,cells
R11251 T13519 T13520 compound +,cells
R11252 T13520 T13517 pobj cells,on
R11253 T13521 T13516 punct .,gated
R11254 T13522 T13523 amod Unstained,cells
R11255 T13523 T13524 nsubj cells,served
R11256 T13524 T13524 ROOT served,served
R11205 T13473 T13472 punct ",",medium
R11206 T13474 T13476 nummod 100,LPS
R11207 T13475 T13476 compound ng/ml,LPS
R11208 T13476 T13472 conj LPS,medium
R11209 T13477 T13476 cc or,LPS
R11498 T13813 T13814 amod Defective,production
R11499 T13814 T13814 ROOT production,production
R11500 T13815 T13814 prep of,production
R11501 T13816 T13817 amod pro-inflammatory,mediators
R11502 T13817 T13815 pobj mediators,of
R11503 T13818 T13814 prep from,production
R11504 T13819 T13820 amod stimulated,cpdm
R11505 T13820 T13821 compound cpdm,BMDC
R11506 T13821 T13818 pobj BMDC,from
R11507 T13822 T13814 punct .,production
R11508 T13823 T13844 punct (,washed
R11509 T13824 T13844 intj A,washed
R11585 T13900 T13902 nummod 2,104
R11586 T13901 T13902 nummod ×,104
R11587 T13902 T13896 appos 104,1
R11588 T13903 T13896 punct ",",1
R11589 T13904 T13896 cc and,1
R11590 T13905 T13906 nummod 4,×
R11591 T13906 T13918 nsubjpass ×,used
R11592 T13907 T13908 nummod 104,cells
R11593 T13908 T13906 appos cells,×
R11594 T13909 T13908 prep in,cells
R11595 T13910 T13913 nummod 0.1,medium
R11596 T13911 T13913 nmod mL,medium
R11597 T13912 T13913 amod complete,medium
R11598 T13913 T13909 pobj medium,in
R11599 T13914 T13908 punct ),cells
R11600 T13915 T13908 prep of,cells
R11601 T13916 T13915 pobj BMDC,of
R11602 T13917 T13918 auxpass were,used
R11603 T13918 T13918 ROOT used,used
R11604 T13919 T13922 nummod for100,stimulation
R11605 T13920 T13922 compound ng/mL,stimulation
R11606 T13921 T13922 compound LPS,stimulation
R11607 T13922 T13918 dobj stimulation,used
R11608 T13923 T13918 punct .,used
R11609 T13924 T13938 prep After,measured
R11610 T13925 T13926 nummod 24,hours
R11611 T13926 T13924 pobj hours,After
R11612 T13927 T13938 punct ",",measured
R11613 T13928 T13929 det the,amounts
R11614 T13929 T13938 nsubjpass amounts,measured
R11615 T13930 T13929 prep of,amounts
R11616 T13931 T13930 pobj IL12P70,of
R11617 T13932 T13931 cc and,IL12P70
R11618 T13933 T13931 conj IL6,IL12P70
R11619 T13934 T13929 prep from,amounts
R11620 T13935 T13936 det the,supernatant
R11621 T13936 T13934 pobj supernatant,from
R11622 T13937 T13938 auxpass were,measured
R11623 T13938 T13938 ROOT measured,measured
R11624 T13939 T13938 punct .,measured
R11625 T13940 T13941 punct (,G
R11626 T13941 T13941 ROOT G,G
R11627 T13942 T13941 punct ),G
R11628 T13943 T13945 det The,mice
R11629 T13944 T13945 compound cpdm,mice
R11630 T13945 T13950 nsubj mice,had
R11631 T13946 T13945 acl rescued,mice
R11632 T13947 T13946 agent by,rescued
R11633 T13948 T13949 amod Sharpin-containing,BAC
R11634 T13949 T13947 pobj BAC,by
R11635 T13950 T13950 ROOT had,had
R11636 T13951 T13952 amod complete,remission
R11637 T13952 T13950 dobj remission,had
R11638 T13953 T13952 prep of,remission
R11639 T13954 T13957 det the,[
R11640 T13955 T13956 amod inflammatory,phenotype
R11641 T13956 T13957 compound phenotype,[
R11642 T13957 T13953 pobj [,of
R11643 T13958 T13957 nummod 2,[
R11644 T13959 T13957 appos ],[
R11645 T13960 T13950 punct .,had
R11646 T13961 T13962 nsubj BMDC,developed
R11647 T13962 T13962 ROOT developed,developed
R11648 T13963 T13962 prep from,developed
R11649 T13964 T13963 pobj cpdm,from
R11650 T13965 T13962 cc and,developed
R11651 T13966 T13962 conj rescued,developed
R11652 T13967 T13968 compound cpdm,mice
R11653 T13968 T13970 nsubjpass mice,plated
R11654 T13969 T13970 auxpass were,plated
R11655 T13970 T13966 ccomp plated,rescued
R11656 T13971 T13970 punct (,plated
R11657 T13972 T13975 nummod 1,cells
R11658 T13973 T13975 nummod ×,cells
R11659 T13974 T13975 nummod 106,cells
R11949 T14307 T14309 nmod /,poly
R11950 T14308 T14309 compound ml,poly
R11951 T14309 T14310 nmod poly,I
R11952 T14310 T14294 nummod I,LPS
R11953 T14311 T14312 punct :,C
R11954 T14312 T14310 acl C,I
R11955 T14313 T14314 punct (,B
R11956 T14314 T14312 appos B,C
R11957 T14315 T14314 punct ",",B
R11958 T14316 T14314 appos D,B
R11959 T14317 T14316 punct ",",D
R11960 T14318 T14316 appos F,D
R11961 T14319 T14316 punct ",",D
R12189 T14585 T14584 nummod TLR4,Normal
R12190 T14586 T14585 cc and,TLR4
R12191 T14587 T14585 conj MD2,TLR4
R12192 T14588 T14590 nsubj expression,decreased
R12193 T14589 T14588 cc and,expression
R12194 T14590 T14590 ROOT decreased,decreased
R12195 T14591 T14592 compound IL10,secretion
R12196 T14592 T14590 dobj secretion,decreased
R12197 T14593 T14592 cc and,secretion
R12198 T14594 T14595 compound A20,expression
R12199 T14595 T14592 conj expression,secretion
R12200 T14596 T14595 prep by,expression
R12201 T14597 T14598 compound cpdm,BMDC
R12202 T14598 T14596 pobj BMDC,by
R12203 T14599 T14590 punct .,decreased
R12204 T14600 T14614 punct (,labeled
R12205 T14601 T14604 nmod A,WT
R12206 T14602 T14601 punct ),A
R12207 T14603 T14604 compound Unstimulated,WT
R12208 T14604 T14614 nsubjpass WT,labeled
R12209 T14605 T14604 cc and,WT
R12210 T14606 T14607 compound cpdm,BMDC
R12211 T14607 T14604 conj BMDC,WT
R12212 T14608 T14609 punct (,5
R12213 T14609 T14611 nummod 5,105
R12214 T14610 T14611 nummod ×,105
R12215 T14611 T14604 appos 105,WT
R12216 T14612 T14604 punct ),WT
R12217 T14613 T14614 auxpass were,labeled
R12218 T14614 T14614 ROOT labeled,labeled
R12219 T14615 T14614 prep with,labeled
R12220 T14616 T14618 nmod PE-labeled,/
R12221 T14617 T14618 amod anti-TLR4,/
R12222 T14618 T14615 pobj /,with
R12223 T14619 T14618 nummod MD2,/
R12224 T14620 T14618 cc or,/
R12225 T14621 T14618 conj anti-CD14,/
R12226 T14622 T14621 cc and,anti-CD14
R12227 T14623 T14624 advmod then,subject
R12228 T14624 T14621 conj subject,anti-CD14
R12229 T14625 T14626 aux to,flow
R12230 T14626 T14624 xcomp flow,subject
R12231 T14627 T14628 amod cytometry,analysis
R11257 T13525 T13524 prep as,served
R11258 T13526 T13527 amod negative,controls
R11259 T13527 T13525 pobj controls,as
R11260 T13528 T13524 punct .,served
R11261 T13529 T13530 nsubj Results,are
R11262 T13530 T13530 ROOT are,are
R11263 T13531 T13530 attr representative,are
R11264 T13532 T13531 prep of,representative
R11265 T13533 T13535 nummod two,experiments
R11266 T13534 T13535 amod independent,experiments
R11267 T13535 T13532 pobj experiments,of
R11268 T13536 T13530 punct .,are
R11510 T13825 T13844 meta D,washed
R11511 T13826 T13825 punct ),D
R11512 T13827 T13828 compound Cultured,WT
R11513 T13828 T13825 conj WT,D
R11514 T13829 T13828 cc and,WT
R11515 T13830 T13831 compound cpdm,BMDC
R11516 T13831 T13828 conj BMDC,WT
R11517 T13832 T13833 punct (,1
R11518 T13833 T13836 nummod 1,cells
R11519 T13834 T13836 nummod ×,cells
R11520 T13835 T13836 nummod 105,cells
R11521 T13836 T13844 nsubjpass cells,washed
R11522 T13837 T13836 prep in,cells
R11523 T13838 T13841 nummod 0.1,medium
R11524 T13839 T13841 amod ml,medium
R11525 T13840 T13841 amod complete,medium
R11526 T13841 T13837 pobj medium,in
R11527 T13842 T13844 punct ),washed
R11528 T13843 T13844 auxpass were,washed
R11529 T13844 T13844 ROOT washed,washed
R11530 T13845 T13844 cc and,washed
R11531 T13846 T13844 conj stimulated,washed
R11532 T13847 T13846 prep with,stimulated
R11533 T13848 T13847 pobj medium,with
R11534 T13849 T13848 punct ",",medium
R11535 T13850 T13852 nummod 100,LPS
R11536 T13851 T13852 compound ng/ml,LPS
R11537 T13852 T13848 conj LPS,medium
R11538 T13853 T13852 cc or,LPS
R11539 T13854 T13858 nummod 25,poly
R11540 T13855 T13858 nmod µg,poly
R11541 T13856 T13858 nmod /,poly
R11542 T13857 T13858 compound ml,poly
R11543 T13858 T13852 conj poly,LPS
R11544 T13859 T13858 nummod I,poly
R11545 T13860 T13858 punct :,poly
R11546 T13861 T13858 appos C,poly
R11547 T13862 T13852 prep for,LPS
R11548 T13863 T13864 nummod 24,hours
R11549 T13864 T13862 pobj hours,for
R11550 T13865 T13844 punct .,washed
R11551 T13866 T13868 nsubjpass Supernatants,collected
R11552 T13867 T13868 auxpass were,collected
R11553 T13868 T13868 ROOT collected,collected
R11554 T13869 T13868 prep for,collected
R11555 T13870 T13869 pobj ELISA,for
R11556 T13871 T13870 prep of,ELISA
R11557 T13872 T13871 pobj IL12P70,of
R11558 T13873 T13872 punct ",",IL12P70
R11559 T13874 T13872 conj IL6,IL12P70
R11560 T13875 T13874 punct ",",IL6
R11561 T13876 T13874 cc and,IL6
R11562 T13877 T13874 conj GMCSF,IL6
R11563 T13878 T13877 punct ",",GMCSF
R11564 T13879 T13869 cc and,for
R11565 T13880 T13869 conj for,for
R11566 T13881 T13880 pobj quantification,for
R11567 T13882 T13881 prep of,quantification
R11568 T13883 T13884 amod nitric,oxide
R11569 T13884 T13882 pobj oxide,of
R11570 T13885 T13884 punct (,oxide
R11571 T13886 T13880 intj NO,for
R11572 T13887 T13880 punct ),for
R11573 T13888 T13868 punct .,collected
R11574 T13889 T13891 punct (,F
R11575 T13890 T13891 compound E,F
R11576 T13891 T13891 ROOT F,F
R11577 T13892 T13891 punct ),F
R11578 T13893 T13894 compound Gradient,numbers
R11579 T13894 T13918 nsubjpass numbers,used
R11580 T13895 T13896 punct (,1
R11581 T13896 T13918 meta 1,used
R11582 T13897 T13898 nummod ×,104
R11583 T13898 T13896 appos 104,1
R11584 T13899 T13902 punct ",",104
R11660 T13975 T13970 appos cells,plated
R11661 T13976 T13975 prep in,cells
R11662 T13977 T13978 nummod 0.3,mL
R11663 T13978 T13976 pobj mL,in
R11664 T13979 T13975 punct ),cells
R11665 T13980 T13970 cc and,plated
R11666 T13981 T13970 conj stimulated,plated
R11667 T13982 T13981 prep with,stimulated
R11668 T13983 T13985 nummod 100,LPS
R11669 T13984 T13985 compound ng/mL,LPS
R11670 T13985 T13982 pobj LPS,with
R11671 T13986 T13962 punct .,developed
R11672 T13987 T13993 prep After,collected
R11673 T13988 T13989 nummod 24,hours
R11674 T13989 T13987 pobj hours,After
R11675 T13990 T13993 punct ",",collected
R11962 T14320 T14316 appos H,D
R11963 T14321 T14314 punct ),B
R11964 T14322 T14288 punct .,washed
R11965 T14323 T14334 prep At,extracted
R11966 T14324 T14323 pobj 0,At
R11967 T14325 T14334 punct ",",extracted
R11968 T14326 T14329 nummod 1,hours
R11969 T14327 T14326 cc and,1
R11970 T14328 T14326 conj 2,1
R11971 T14329 T14334 npadvmod hours,extracted
R11972 T14330 T14334 punct ",",extracted
R11973 T14331 T14332 amod total,RNA
R11974 T14332 T14334 nsubjpass RNA,extracted
R11975 T14333 T14334 auxpass was,extracted
R11976 T14334 T14334 ROOT extracted,extracted
R11977 T14335 T14334 cc and,extracted
R11978 T14336 T14372 nmod subject,mRNA
R11979 T14337 T14336 prep to,subject
R11980 T14338 T14337 pobj qRT-PCR,to
R11981 T14339 T14340 aux to,measure
R11982 T14340 T14336 xcomp measure,subject
R11983 T14341 T14342 det the,expression
R11984 T14342 T14340 dobj expression,measure
R11985 T14343 T14342 prep of,expression
R11986 T14344 T14343 pobj Il12p40,of
R11987 T14345 T14346 punct (,A
R11988 T14346 T14348 advmod A,B
R11989 T14347 T14346 punct ",",A
R11990 T14348 T14344 appos B,Il12p40
R11991 T14349 T14344 punct ),Il12p40
R11992 T14350 T14344 punct ",",Il12p40
R11993 T14351 T14344 conj Il6,Il12p40
R11994 T14352 T14353 punct (,C
R11995 T14353 T14344 appos C,Il12p40
R12239 T14635 T14633 pobj controls,as
R12240 T14636 T14632 punct .,serve
R12241 T14637 T14666 punct (,washed
R12242 T14638 T14666 nsubjpass B,washed
R12243 T14639 T14697 punct ),C
R12244 T14640 T14641 compound Cultured,WT
R12245 T14641 T14852 nsubjpass WT,washed
R12246 T14642 T14641 cc and,WT
R12247 T14643 T14644 compound cpdm,BMDC
R12248 T14644 T14641 conj BMDC,WT
R12249 T14645 T14646 punct (,5
R12250 T14646 T14844 nummod 5,cells
R12251 T14647 T14844 nummod ×,cells
R12252 T14648 T14638 punct ),B
R12253 T14649 T14650 compound Cultured,WT
R12254 T14650 T14638 appos WT,B
R12255 T14651 T14650 cc and,WT
R12256 T14652 T14653 compound cpdm,BMDC
R12257 T14653 T14650 conj BMDC,WT
R12258 T14654 T14655 punct (,1
R12232 T14628 T14626 dobj analysis,flow
R12233 T14629 T14614 punct .,labeled
R12234 T14630 T14631 amod Unstained,cells
R12235 T14631 T14632 nsubj cells,serve
R12236 T14632 T14632 ROOT serve,serve
R12237 T14633 T14632 prep as,serve
R12238 T14634 T14635 amod negative,controls
R12453 T14843 T14844 nummod 105,cells
R12454 T14844 T14641 appos cells,WT
R12455 T14845 T14844 prep in,cells
R12456 T14846 T14847 nummod 0.2,ml
R12457 T14847 T14849 amod ml,medium
R12458 T14848 T14849 amod complete,medium
R12459 T14849 T14845 pobj medium,in
R12460 T14850 T14844 punct ),cells
R12461 T14851 T14852 auxpass were,washed
R12462 T14852 T14852 ROOT washed,washed
R12463 T14860 T14852 cc and,washed
R12464 T14861 T14852 conj stimulated,washed
R12465 T14862 T14861 prep with,stimulated
R12466 T14863 T14865 nummod 100,LPS
R12467 T14864 T14865 compound ng/ml,LPS
R12468 T14865 T14862 pobj LPS,with
R12469 T14866 T14865 cc or,LPS
R12470 T14867 T14871 nummod 25,poly
R12471 T14868 T14871 nmod µg,poly
R12472 T14869 T14871 nmod /,poly
R12473 T14870 T14871 compound ml,poly
R12474 T14871 T14874 compound poly,C
R12475 T14872 T14874 compound I,C
R12476 T14873 T14872 punct :,I
R12477 T14874 T14865 conj C,LPS
R12478 T14875 T14852 punct .,washed
R12479 T14876 T14888 prep At,extracted
R12480 T14877 T14883 nummod 0,hours
R12481 T14878 T14879 dobj ",",1
R12482 T14879 T14877 appos 1,0
R12483 T14880 T14877 punct ",",0
R12484 T14881 T14877 cc and,0
R12485 T14882 T14877 conj 2,0
R12486 T14883 T14876 pobj hours,At
R12487 T14884 T14888 punct ",",extracted
R12488 T14885 T14886 amod total,RNA
R12489 T14886 T14888 nsubjpass RNA,extracted
R12490 T14887 T14888 auxpass was,extracted
R12491 T14888 T14888 ROOT extracted,extracted
R12492 T14889 T14888 cc and,extracted
R12493 T14890 T14888 conj subject,extracted
R12494 T14891 T14890 prep to,subject
R12495 T14892 T14891 pobj qRT-PCR,to
R12496 T14893 T14894 aux to,measure
R12497 T14894 T14890 xcomp measure,subject
R12498 T14895 T14896 det the,production
R12499 T14896 T14894 dobj production,measure
R12500 T14897 T14896 prep of,production
R12501 T14898 T14897 pobj A20,of
R12502 T14899 T14888 punct .,extracted
R12689 T15155 T15152 pobj LPS,with
R12690 T15156 T15157 punct (,A
R12691 T15157 T15155 appos A,LPS
R12692 T15158 T15157 punct ),A
R12693 T15159 T15155 cc or,LPS
R12694 T15160 T15162 quantmod 25,/
R12695 T15161 T15162 quantmod µg,/
R12696 T15162 T15155 conj /,LPS
R12697 T15163 T15164 compound mL,poly
R12698 T15164 T15165 dep poly,I
R12699 T15165 T15162 meta I,/
R12700 T15166 T15167 punct :,C
R12701 T15167 T15170 nmod C,)
R12702 T15168 T15169 punct (,B
R12703 T15169 T15167 appos B,C
R12704 T15170 T15165 punct ),I
R12705 T15171 T15151 punct .,stimulated
R12706 T15172 T15186 prep At,obtained
R12707 T15173 T15172 pobj 0,At
R12708 T15174 T15177 punct ",",30
R12709 T15175 T15177 quantmod 15,30
R12710 T15176 T15177 punct ",",30
R12711 T15177 T15173 appos 30,0
R12712 T15178 T15177 punct ",",30
R12713 T15179 T15177 cc and,30
R12714 T15180 T15181 nummod 60,minutes
R12715 T15181 T15186 npadvmod minutes,obtained
R12716 T15182 T15186 punct ",",obtained
R12717 T15183 T15184 amod whole-cell,lysates
R12718 T15184 T15186 nsubjpass lysates,obtained
R12719 T15185 T15186 auxpass were,obtained
R12720 T15186 T15186 ROOT obtained,obtained
R12721 T15187 T15186 cc and,obtained
R12722 T15188 T15186 conj subject,obtained
R12723 T15189 T15188 prep to,subject
R12724 T15190 T15189 pobj immunoblots,to
R12725 T15191 T15190 prep with,immunoblots
R12726 T15192 T15191 pobj antibodies,with
R12727 T15193 T15190 prep against,immunoblots
R12728 T15194 T15193 pobj proteins,against
R12729 T15195 T15194 acl involved,proteins
R12730 T15196 T15195 prep in,involved
R12731 T15197 T15196 pobj NF-κB,in
R12732 T15198 T15197 punct ",",NF-κB
R12733 T15199 T15197 conj TBK1/IRF3,NF-κB
R12734 T15200 T15199 punct ",",TBK1/IRF3
R12735 T15201 T15199 conj ERK1/2,TBK1/IRF3
R12736 T15202 T15201 punct ",",ERK1/2
R12737 T15203 T15201 cc and,ERK1/2
R12738 T15204 T15206 nummod p38,pathways
R12739 T15205 T15206 compound signaling,pathways
R12740 T15206 T15197 appos pathways,NF-κB
R12741 T15207 T15186 punct .,obtained
R12742 T15208 T15210 nsubjpass Beta-actin,used
R12743 T15209 T15210 auxpass was,used
R12744 T15210 T15210 ROOT used,used
R12745 T15211 T15210 prep as,used
R12746 T15212 T15211 pcomp loading,as
R12747 T15213 T15212 dobj control,loading
R12748 T15214 T15210 punct .,used
R12749 T15215 T15225 punct (,p-IκBα
R12750 T15216 T15219 nmod C,levels
R12751 T15217 T15216 punct ),C
R12752 T15218 T15219 amod Cellular,levels
R12753 T15219 T15225 nsubj levels,p-IκBα
R12754 T15220 T15219 prep of,levels
R12755 T15221 T15222 compound p-IKK1,/
R12756 T15222 T15220 pobj /,of
R12757 T15223 T15222 nummod 2,/
R12758 T15224 T15222 cc and,/
R12759 T15225 T15225 ROOT p-IκBα,p-IκBα
R12760 T15226 T15225 prep in,p-IκBα
R12761 T15227 T15226 pobj LPS,in
R12762 T15228 T15225 punct -,p-IκBα
R12763 T15229 T15225 cc or,p-IκBα
R13073 T15615 T15614 prep of,secretion
R13074 T15616 T15615 pobj IFNγ,of
R13075 T15617 T15616 punct ",",IFNγ
R13076 T15618 T15616 conj IL4,IFNγ
R13077 T15619 T15618 punct ",",IL4
R13078 T15620 T15618 cc and,IL4
R13079 T15621 T15618 conj IL2,IL4
R13080 T15622 T15623 auxpass were,measured
R13081 T15623 T15611 conj measured,collected
R13082 T15624 T15623 agent by,measured
R13083 T15625 T15624 pobj ELISA,by
R13084 T15626 T15623 punct .,measured
R13085 T15627 T15628 amod Negative,controls
R13086 T15628 T15629 nsubj controls,are
R13087 T15629 T15653 ccomp are,stimulated
R13088 T15630 T15629 attr 1,are
R13089 T15631 T15630 punct ),1
R13090 T15632 T15629 conj stimulated,are
R13091 T15633 T15632 dobj BMDC,stimulated
R12259 T14655 T14653 nummod 1,BMDC
R12260 T14656 T14658 nummod ×,cells
R12261 T14657 T14658 nummod 105,cells
R12262 T14658 T14638 appos cells,B
R12263 T14659 T14658 prep in,cells
R12264 T14660 T14663 nummod 0.1,medium
R12265 T14661 T14663 amod ml,medium
R12266 T14662 T14663 amod complete,medium
R12267 T14663 T14659 pobj medium,in
R12268 T14664 T14638 punct ),B
R12269 T14665 T14666 auxpass were,washed
R12270 T14666 T14666 ROOT washed,washed
R12271 T14667 T14666 cc and,washed
R12272 T14668 T14666 conj stimulated,washed
R12273 T14669 T14668 prep with,stimulated
R12274 T14670 T14669 pobj medium,with
R12275 T14671 T14670 punct ",",medium
R12276 T14672 T14674 nummod 100,LPS
R12277 T14673 T14674 compound ng/ml,LPS
R12278 T14674 T14670 conj LPS,medium
R12279 T14675 T14674 cc or,LPS
R12280 T14676 T14680 nummod 25,poly
R12281 T14677 T14680 nmod µg,poly
R12282 T14678 T14680 nmod /,poly
R12283 T14679 T14680 compound ml,poly
R12284 T14680 T14674 conj poly,LPS
R12285 T14681 T14683 nsubj I,C
R12286 T14682 T14683 punct :,C
R12287 T14683 T14683 ROOT C,C
R12288 T14684 T14683 prep for,C
R12289 T14685 T14686 nummod 24,hours
R12290 T14686 T14684 pobj hours,for
R12291 T14687 T14683 punct .,C
R12292 T14688 T14690 nsubjpass Supernatants,collected
R12293 T14689 T14690 auxpass were,collected
R12294 T14690 T14690 ROOT collected,collected
R12295 T14691 T14690 prep for,collected
R12296 T14692 T14691 pobj ELISA,for
R12297 T14693 T14692 prep of,ELISA
R12298 T14694 T14693 pobj IL10,of
R12299 T14695 T14690 punct .,collected
R12300 T14696 T14641 punct (,WT
R12301 T14697 T14641 compound C,WT
R12661 T15127 T15130 nsubj Inhibition,signaling
R12662 T15128 T15127 prep of,Inhibition
R12663 T15129 T15128 pobj NF-κB,of
R12664 T15130 T15130 ROOT signaling,signaling
R12665 T15131 T15130 prep in,signaling
R12666 T15132 T15133 compound cpdm,BMDC
R12667 T15133 T15131 pobj BMDC,in
R12668 T15134 T15130 punct .,signaling
R12669 T15135 T15143 nmod WT,cells
R12670 T15136 T15135 cc and,WT
R12671 T15137 T15138 compound cpdm,BMDC
R12672 T15138 T15135 conj BMDC,WT
R12673 T15139 T15140 punct (,2
R12674 T15140 T15143 nummod 2,cells
R12675 T15141 T15143 nummod ×,cells
R12676 T15142 T15143 nummod 106,cells
R12677 T15143 T15151 nsubjpass cells,stimulated
R12678 T15144 T15143 prep in,cells
R12679 T15145 T15148 nummod 0.5,medium
R12680 T15146 T15148 nmod mL,medium
R12681 T15147 T15148 amod complete,medium
R12682 T15148 T15144 pobj medium,in
R12683 T15149 T15143 punct ),cells
R12684 T15150 T15151 auxpass were,stimulated
R12685 T15151 T15151 ROOT stimulated,stimulated
R12686 T15152 T15151 prep with,stimulated
R12687 T15153 T15155 nummod 100,LPS
R12688 T15154 T15155 compound ng/mL,LPS
R12764 T15230 T15236 intj poly,quantitated
R12765 T15231 T15236 nsubjpass I,quantitated
R12766 T15232 T15236 punct :,quantitated
R12767 T15233 T15234 compound C-stimulated,BMDC
R12768 T15234 T15236 nsubjpass BMDC,quantitated
R12769 T15235 T15236 auxpass were,quantitated
R12770 T15236 T15236 ROOT quantitated,quantitated
R12771 T15237 T15236 prep with,quantitated
R12772 T15238 T15237 pobj ImageJ,with
R12773 T15239 T15238 punct (,ImageJ
R12774 T15240 T15238 appos NIH,ImageJ
R12775 T15241 T15238 punct ),ImageJ
R12776 T15242 T15236 cc and,quantitated
R12777 T15243 T15236 conj presented,quantitated
R12778 T15244 T15243 prep as,presented
R12779 T15245 T15246 compound trend,lines
R12780 T15246 T15244 pobj lines,as
R12781 T15247 T15236 punct .,quantitated
R12782 T15248 T15249 nsubj Results,are
R12783 T15249 T15249 ROOT are,are
R12784 T15250 T15249 attr representative,are
R12785 T15251 T15250 prep of,representative
R12786 T15252 T15253 advmod at,least
R12787 T15253 T15254 advmod least,two
R12788 T15254 T15256 nummod two,experiments
R12789 T15255 T15256 amod independent,experiments
R12790 T15256 T15251 pobj experiments,of
R12791 T15257 T15249 punct .,are
R12968 T15510 T15513 advcl Stimulated,induced
R12969 T15511 T15512 compound cpdm,BMDC
R12970 T15512 T15513 nsubj BMDC,induced
R12971 T15513 T15513 ROOT induced,induced
R12972 T15514 T15516 amod Th2-biased,production
R12973 T15515 T15516 compound cytokine,production
R12974 T15516 T15513 dobj production,induced
R12975 T15517 T15516 prep from,production
R12976 T15518 T15522 amod naïve,cells
R12977 T15519 T15522 compound CD4,cells
R12978 T15520 T15522 compound +,cells
R12979 T15521 T15522 compound T,cells
R12980 T15522 T15517 pobj cells,from
R12981 T15523 T15513 punct .,induced
R12982 T15524 T15532 nmod WT,cells
R12983 T15525 T15524 cc and,WT
R12984 T15526 T15527 compound cpdm,BMDC
R12985 T15527 T15524 conj BMDC,WT
R12986 T15528 T15529 punct (,5
R12987 T15529 T15532 nummod 5,cells
R12988 T15530 T15532 nummod ×,cells
R12989 T15531 T15532 nummod 104,cells
R12990 T15532 T15532 ROOT cells,cells
R12991 T15533 T15532 prep in,cells
R12992 T15534 T15537 nummod 0.1,medium
R12993 T15535 T15537 nmod mL,medium
R12994 T15536 T15537 amod complete,medium
R12995 T15537 T15533 pobj medium,in
R12996 T15538 T15532 punct ;,cells
R12997 T15539 T15532 appos MHC,cells
R12998 T15540 T15545 nsubjpass haplotype,incubated
R12999 T15541 T15540 punct :,haplotype
R13000 T15542 T15540 appos H-2b,haplotype
R13001 T15543 T15540 punct ),haplotype
R13002 T15544 T15545 auxpass were,incubated
R13003 T15545 T15545 ROOT incubated,incubated
R13004 T15546 T15545 prep with,incubated
R13005 T15547 T15546 pobj medium,with
R13006 T15548 T15547 punct ",",medium
R13007 T15549 T15551 nummod 100,LPS
R13008 T15550 T15551 compound ng/mL,LPS
R13009 T15551 T15547 conj LPS,medium
R13010 T15552 T15551 punct ",",LPS
R13011 T15553 T15555 quantmod 25,/
R13012 T15554 T15555 quantmod µg,/
R13013 T15555 T15557 npadvmod /,poly
R13014 T15556 T15557 compound mL,poly
R13015 T15557 T15558 dep poly,I
R13016 T15558 T15545 advcl I,incubated
R13017 T15559 T15558 punct :,I
R13018 T15560 T15558 appos C,I
R13019 T15561 T15560 cc or,C
R13020 T15562 T15564 nummod 5,/
R13021 T15563 T15564 compound µg,/
R13022 T15564 T15566 compound /,Pam3CYS
R13023 T15565 T15566 compound mL,Pam3CYS
R13024 T15566 T15560 conj Pam3CYS,C
R13025 T15567 T15545 punct .,incubated
R13026 T15568 T15574 prep After,washed
R13092 T15634 T15632 prep without,stimulated
R13093 T15635 T15634 pobj co-culture,without
R13094 T15636 T15632 prep with,stimulated
R13095 T15637 T15641 amod allogeneic,cells
R13096 T15638 T15641 compound CD4,cells
R13097 T15639 T15641 nummod +,cells
R13098 T15640 T15641 compound T,cells
R13099 T15641 T15636 pobj cells,with
R13100 T15642 T15653 punct ;,stimulated
R13101 T15643 T15649 meta 2,cells
R13102 T15644 T15643 punct ),2
R13103 T15645 T15649 amod allogeneic,cells
R13104 T15646 T15649 compound CD4,cells
R13105 T15647 T15649 nummod +,cells
R13106 T15648 T15649 compound T,cells
R13107 T15649 T15653 nsubj cells,stimulated
R13108 T15650 T15649 prep without,cells
R13109 T15651 T15650 pobj co-culture,without
R13110 T15652 T15649 prep with,cells
R13111 T15653 T15653 ROOT stimulated,stimulated
R13112 T15654 T15653 dobj BMDC,stimulated
R13113 T15655 T15653 punct .,stimulated
R13114 T15656 T15661 nsubj Samples,had
R13115 T15657 T15656 prep from,Samples
R13116 T15658 T15660 det both,controls
R13117 T15659 T15660 amod negative,controls
R13118 T15660 T15657 pobj controls,from
R13119 T15661 T15661 ROOT had,had
R13120 T15662 T15664 det no,production
R13121 T15663 T15664 amod detectable,production
R13122 T15664 T15661 dobj production,had
R13123 T15665 T15664 prep of,production
R13124 T15666 T15668 det the,cytokines
R5447 T6841 T6841 ROOT diminished,diminished
R5448 T6842 T6843 det the,capacity
R5449 T6843 T6841 dobj capacity,diminished
R5450 T6844 T6843 prep of,capacity
R5451 T6845 T6844 pobj BMDC,of
R5452 T6846 T6847 aux to,secrete
R5453 T6847 T6841 advcl secrete,diminished
R5454 T6848 T6849 amod inflammatory,cytokines
R5455 T6849 T6847 dobj cytokines,secrete
R5456 T6850 T6849 cc and,cytokines
R5457 T6851 T6852 amod nitric,oxide
R5458 T6852 T6849 conj oxide,cytokines
R5767 T7161 T7162 nummod Th1,differentiation
R5768 T7162 T7160 dobj differentiation,polarized
R5769 T7163 T7160 punct ",",polarized
R5770 T7164 T7157 cc but,stimulated
R5772 T7166 T7157 conj supported,stimulated
R5773 T7167 T7168 det the,development
R5775 T7169 T7168 prep of,development
R5777 T7171 T7172 compound effector,cells
R5779 T7173 T7157 punct .,stimulated
R5781 T7175 T7174 prep with,Combined
R5782 T7176 T7178 det the,effect
R5784 T7178 T7175 pobj effect,with
R5785 T7179 T7178 prep of,effect
R5787 T7181 T7179 pobj treatment,of
R5789 T7183 T7184 det the,phenotype
R5790 T7184 T7182 pobj phenotype,on
R5792 T7186 T7187 det these,mice
R5794 T7188 T7190 nmod [,]
R5795 T7189 T7190 nummod 5,]
R5797 T7191 T7190 punct ",",]
R5799 T7193 T7193 ROOT suggests,suggests
R5800 T7194 T7203 mark that,caused
R5802 T7196 T7198 amod Th2-biased,inflammation
R5803 T7197 T7198 amod systemic,inflammation
R11693 T14008 T14016 ccomp *,0.005
R11695 T14010 T14011 compound <,0.01
R11697 T14012 T14016 punct ;,0.005
R11698 T14013 T14016 punct **,0.005
R11700 T14015 T14016 nmod <,0.005
R11702 T14017 T14016 punct .,0.005
R11904 T14262 T14263 compound mRNA,levels
R11906 T14264 T14263 prep of,levels
R11908 T14266 T14264 pobj cytokines,of
R11909 T14267 T14263 prep from,levels
R11911 T14269 T14267 pobj BMDC,from
R11913 T14271 T14272 compound Cultured,WT
R13143 T15685 T15687 punct *,<
R13145 T15687 T15688 compound <,0.05
R13147 T15689 T15688 punct .,0.05
R43 T127 T119 punct ",",Is
R44 T128 T119 cc and,Is
R45 T129 T157 nsubj Induction,result
R46 T130 T129 prep of,Induction
R47 T131 T132 compound Th1,Differentiation
R48 T132 T130 pobj Differentiation,of
R49 T133 T129 agent by,Induction
R50 T134 T136 compound Dendritic,Spontaneous
R51 T135 T136 compound Cells,Spontaneous
R52 T136 T133 pobj Spontaneous,by
R53 T137 T129 conj mutations,Induction
R54 T138 T137 prep of,mutations
R55 T139 T140 det the,Sharpin
R56 T140 T138 pobj Sharpin,of
R57 T141 T145 punct (,protein
R58 T142 T145 amod SHANK-associated,protein
R59 T143 T145 compound RH,protein
R60 T144 T145 compound domain-interacting,protein
R61 T145 T140 appos protein,Sharpin
R62 T146 T148 punct ",",aliases
R63 T147 T148 amod other,aliases
R64 T148 T137 conj aliases,mutations
R65 T149 T148 punct :,aliases
R66 T150 T148 conj Rbckl1,aliases
R67 T151 T150 punct ",",Rbckl1
R68 T152 T154 nmod Sipl1,gene
R69 T153 T152 punct ),Sipl1
R70 T154 T157 nsubj gene,result
R4904 T6083 T6081 pobj production,in
R5677 T7071 T7071 ROOT is,is
R5678 T7072 T7074 det a,feature
R5679 T7073 T7074 amod prominent,feature
R5680 T7074 T7071 attr feature,is
R5681 T7075 T7074 prep of,feature
R5682 T7076 T7078 det the,lesions
R5683 T7077 T7078 compound skin,lesions
R5684 T7078 T7075 pobj lesions,of
R5685 T7079 T7078 prep in,lesions
R5686 T7080 T7081 compound cpdm,mice
R5687 T7081 T7079 pobj mice,in
R5688 T7082 T7084 nmod [,]
R5689 T7083 T7084 nummod 33,]
R5690 T7084 T7078 conj ],lesions
R5691 T7085 T7084 cc and,]
R5692 T7086 T7088 nsubjpass this,mediated
R5693 T7087 T7088 auxpass is,mediated
R5694 T7088 T7071 advcl mediated,is
R5720 T7114 T7104 prep by,release
R5721 T7115 T7114 pcomp activating,by
R5722 T7116 T7115 dobj DC,activating
R5723 T7117 T7115 prep through,activating
R5724 T7118 T7119 compound pattern,recognition
R5725 T7119 T7120 compound recognition,receptors
R5726 T7120 T7117 pobj receptors,through
R5728 T7122 T7123 nummod 32,]
R5729 T7123 T7120 appos ],receptors
R5730 T7124 T7123 punct ",",]
R5731 T7125 T7127 nmod [,]
R5732 T7126 T7127 nummod 35,]
R5733 T7127 T7123 conj ],]
R5734 T7128 T7127 punct ",",]
R5735 T7129 T7130 amod such,as
R5736 T7130 T7127 prep as,]
R5737 T7131 T7130 pobj HMGB1,as
R5738 T7132 T7104 advcl recognized,release
R5739 T7133 T7132 agent by,recognized
R5740 T7134 T7137 nummod TLR2/4,]
R5741 T7135 T7137 nmod [,]
R5742 T7136 T7137 nummod 36,]
R5743 T7137 T7133 pobj ],by
R5744 T7138 T7137 punct ",",]
R5745 T7139 T7141 nmod [,]
R5746 T7140 T7141 nummod 37,]
R5747 T7141 T7137 appos ],]
R5748 T7142 T7104 punct .,release
R5749 T7143 T7157 advmod Consistent,stimulated
R5750 T7144 T7143 prep with,Consistent
R5751 T7145 T7149 det the,response
R5752 T7146 T7149 amod impaired,response
R5753 T7147 T7149 nmod Th1,response
R5754 T7148 T7149 amod immune,response
R5755 T7149 T7144 pobj response,with
R5756 T7150 T7149 prep in,response
R5757 T7151 T7152 compound cpdm,mice
R5758 T7152 T7150 pobj mice,in
R5759 T7153 T7155 nmod [,]
R8649 T10497 T10498 punct (,MCD11C20
R8650 T10498 T10496 appos MCD11C20,anti-CD11c
R8651 T10499 T10500 punct ",",CALTAG
R8652 T10500 T10498 appos CALTAG,MCD11C20
R8653 T10501 T10498 punct ),MCD11C20
R8654 T10502 T10498 prep in,MCD11C20
R8655 T10503 T10502 pobj combination,in
R8656 T10504 T10503 prep with,combination
R8657 T10505 T10507 compound PE,anti-CD40
R8658 T10506 T10507 punct -,anti-CD40
R8659 T10507 T10504 pobj anti-CD40,with
R8660 T10508 T10507 punct (,anti-CD40
R8661 T10509 T10507 appos 3/23,anti-CD40
R8662 T10510 T10507 punct ",",anti-CD40
R8663 T10511 T10512 compound BD,Biosciences
R8664 T10512 T10507 appos Biosciences,anti-CD40
R8665 T10513 T10507 punct ),anti-CD40
R8666 T10514 T10507 punct ",",anti-CD40
R8667 T10515 T10507 appos PE-anti-CD80,anti-CD40
R8668 T10516 T10517 punct (,16-10A1
R8669 T10517 T10515 appos 16-10A1,PE-anti-CD80
R13027 T15569 T15570 nummod 24,hours
R13028 T15570 T15568 pobj hours,After
R13029 T15571 T15574 punct ",",washed
R13030 T15572 T15574 nsubjpass cells,washed
R13031 T15573 T15574 auxpass were,washed
R13032 T15574 T15574 ROOT washed,washed
R13033 T15575 T15574 prep with,washed
R13034 T15576 T15575 pobj PBS,with
R13035 T15577 T15574 cc and,washed
R13036 T15578 T15574 conj incubated,washed
R13037 T15579 T15578 prep with,incubated
R13038 T15580 T15581 advmod freshly,isolated
R13039 T15581 T15583 amod isolated,naïve
R13040 T15582 T15583 amod allogeneic,naïve
R13041 T15583 T15587 compound naïve,cells
R13042 T15584 T15587 compound CD4,cells
R13043 T15585 T15587 compound +,cells
R13044 T15586 T15587 compound T,cells
R13045 T15587 T15579 pobj cells,with
R13046 T15588 T15587 punct (,cells
R13047 T15589 T15592 nummod 2.5,cells
R13048 T15590 T15592 nummod ×,cells
R13049 T15591 T15592 nummod 105,cells
R13050 T15592 T15587 appos cells,cells
R13051 T15593 T15592 prep in,cells
R13052 T15594 T15597 nummod 0.1,medium
R13053 T15595 T15597 nmod mL,medium
R13054 T15596 T15597 amod complete,medium
R13055 T15597 T15593 pobj medium,in
R13056 T15598 T15587 punct ;,cells
R13057 T15599 T15600 compound MHC,haplotype
R13058 T15600 T15578 conj haplotype,incubated
R13059 T15601 T15600 punct :,haplotype
R13060 T15602 T15600 appos H-2d,haplotype
R13061 T15603 T15600 punct ),haplotype
R13062 T15604 T15574 punct .,washed
R13063 T15605 T15611 prep After,collected
R13064 T15606 T15607 nummod 5,days
R13065 T15607 T15605 pobj days,After
R13066 T15608 T15611 punct ",",collected
R13067 T15609 T15611 nsubjpass supernatants,collected
R13068 T15610 T15611 auxpass were,collected
R13069 T15611 T15611 ROOT collected,collected
R13070 T15612 T15611 cc and,collected
R13071 T15613 T15614 det the,secretion
R13072 T15614 T15623 nsubjpass secretion,measured
R5771 T7165 T7166 advmod strongly,supported
R5774 T7168 T7166 dobj development,supported
R5776 T7170 T7172 amod Th2,cells
R5778 T7172 T7169 pobj cells,of
R5780 T7174 T7193 advcl Combined,suggests
R5783 T7177 T7178 amod dramatic,effect
R5786 T7180 T7181 nummod IL12,treatment
R5788 T7182 T7181 prep on,treatment
R5791 T7185 T7184 prep of,phenotype
R5793 T7187 T7185 pobj mice,of
R5796 T7190 T7174 nsubj ],Combined
R5798 T7192 T7193 nsubj this,suggests
R5801 T7195 T7198 det the,inflammation
R11692 T14007 T14001 punct .,are
R11694 T14009 T14011 nmod P,0.01
R11696 T14011 T14008 npadvmod 0.01,*
R11699 T14014 T14015 nmod P,<
R11701 T14016 T14016 ROOT 0.005,0.005
R11903 T14261 T14263 amod Decreased,levels
R11905 T14263 T14263 ROOT levels,levels
R11907 T14265 T14266 amod inflammatory,cytokines
R11910 T14268 T14269 compound cpdm,BMDC
R11912 T14270 T14263 punct .,levels
R13142 T15684 T15676 punct .,analyzed
R13144 T15686 T15687 compound P,<
R13146 T15688 T15688 ROOT 0.05,0.05
R2635 T3190 T3189 prep in,resulted
R2636 T3191 T3192 amod increased,expression
R2637 T3192 T3190 pobj expression,in
R2638 T3193 T3192 prep of,expression
R2639 T3194 T3193 pobj CD40,of
R2640 T3195 T3194 punct ",",CD40
R2641 T3196 T3194 conj CD80,CD40
R2642 T3197 T3196 punct ",",CD80
R2643 T3198 T3196 cc and,CD80
R2644 T3199 T3196 conj CD86,CD80
R2645 T3200 T3199 prep on,CD86
R2646 T3201 T3200 pobj BMDC,on
R2647 T3202 T3206 punct ;,was
R2648 T3203 T3206 advmod however,was
R2649 T3204 T3206 punct ",",was
R5760 T7154 T7155 nummod 5,]
R5761 T7155 T7157 nsubj ],stimulated
R5762 T7156 T7157 punct ",",stimulated
R5763 T7157 T7157 ROOT stimulated,stimulated
R5764 T7158 T7157 dobj BMDC,stimulated
R5765 T7159 T7160 advmod weakly,polarized
R5766 T7160 T7157 conj polarized,stimulated
R5804 T7198 T7203 nsubjpass inflammation,caused
R5805 T7199 T7198 prep in,inflammation
R5806 T7200 T7201 compound cpdm,mice
R5808 T7202 T7203 auxpass is,caused
R5809 T7203 T7193 ccomp caused,suggests
R5810 T7204 T7203 agent by,caused
R5811 T7205 T7207 amod reduced,production
R5812 T7206 T7207 compound IL12P70,production
R5813 T7207 T7204 pobj production,by
R5814 T7208 T7207 prep from,production
R5815 T7209 T7208 pobj DC,from
R5816 T7210 T7193 punct .,suggests
R5817 T7211 T7212 det The,importance
R5818 T7212 T7226 nsubjpass importance,demonstrated
R5819 T7213 T7212 prep of,importance
R5820 T7214 T7213 pobj IL12P70,of
R5821 T7215 T7212 prep in,importance
R5822 T7216 T7215 pcomp regulating,in
R5823 T7217 T7221 nmod Th1,responses
R5824 T7218 T7217 cc and,Th1
R5825 T7219 T7217 conj Th2,Th1
R5826 T7220 T7221 amod immune,responses
R5827 T7221 T7216 dobj responses,regulating
R5828 T7222 T7221 prep in,responses
R5829 T7223 T7222 pobj mice,in
R5830 T7224 T7226 auxpass was,demonstrated
R5831 T7225 T7226 advmod clearly,demonstrated
R5832 T7226 T7226 ROOT demonstrated,demonstrated
R5833 T7227 T7226 prep in,demonstrated
R5834 T7228 T7227 pobj IL12P35,in
R6384 T7778 T7777 pobj HOIP,with
R6385 T7779 T7780 aux to,form
R6386 T7780 T7776 advcl form,interacts
R6387 T7781 T7780 dobj LUBAC,form
R6388 T7782 T7783 nsubj that,exerts
R6389 T7783 T7781 relcl exerts,LUBAC
R6390 T7784 T7786 poss its,activity
R6391 T7785 T7786 amod linear-ubiquitin-chain-ligase,activity
R6392 T7786 T7783 dobj activity,exerts
R7844 T9582 T9583 compound mouse,work
R7845 T9583 T9585 nsubjpass work,carried
R7846 T9584 T9585 auxpass was,carried
R7847 T9585 T9585 ROOT carried,carried
R7848 T9586 T9585 prt out,carried
R7849 T9587 T9585 prep in,carried
R7850 T9588 T9589 amod strict,accordance
R7851 T9589 T9587 pobj accordance,in
R7852 T9590 T9589 prep with,accordance
R7853 T9591 T9590 pobj protocols,with
R7854 T9592 T9591 acl approved,protocols
R7855 T9593 T9592 agent by,approved
R7856 T9594 T9600 det the,Committees
R7857 T9595 T9597 compound Institutional,Care
R9604 T11566 T11564 cc and,Gmcsf
R10359 T12472 T12462 punct ),p-p38
R11676 T13991 T13993 nsubjpass supernatants,collected
R11677 T13992 T13993 auxpass were,collected
R11678 T13993 T13993 ROOT collected,collected
R11679 T13994 T13993 prep for,collected
R11680 T13995 T13994 pobj analysis,for
R11681 T13996 T13995 prep of,analysis
R11682 T13997 T13998 compound IL12P70,production
R11683 T13998 T13996 pobj production,of
R8670 T10518 T10517 punct ",",16-10A1
R8671 T10519 T10517 appos eBioscience,16-10A1
R8672 T10520 T10517 punct ),16-10A1
R8673 T10521 T10517 punct ",",16-10A1
R8674 T10522 T10517 conj PE-anti-CD86,16-10A1
R8675 T10523 T10522 punct (,PE-anti-CD86
R8676 T10524 T10525 compound P03,.1
R8677 T10525 T10522 appos .1,PE-anti-CD86
R8678 T10526 T10522 punct ",",PE-anti-CD86
R8679 T10527 T10522 conj eBioscience,PE-anti-CD86
R8680 T10528 T10522 punct ),PE-anti-CD86
R8681 T10529 T10522 punct ",",PE-anti-CD86
R8682 T10530 T10522 conj PE-anti-CD14,PE-anti-CD86
R8683 T10531 T10530 punct (,PE-anti-CD14
R8684 T10532 T10533 compound Sa2,.8
R8685 T10533 T10530 appos .8,PE-anti-CD14
R8686 T10534 T10530 punct ",",PE-anti-CD14
R8687 T10535 T10530 conj eBioscience,PE-anti-CD14
R8688 T10536 T10535 punct ),eBioscience
R8689 T10537 T10515 cc and,PE-anti-CD80
R8690 T10538 T10539 compound biotinylated,anti-TLR4
R8691 T10539 T10515 conj anti-TLR4,PE-anti-CD80
R8692 T10540 T10541 punct (,BioLegend
R8693 T10541 T10539 appos BioLegend,anti-TLR4
R8694 T10542 T10541 punct ),BioLegend
R8695 T10543 T10485 punct .,washed
R8696 T10544 T10545 det The,cells
R8697 T10545 T10553 nsubjpass cells,washed
R11935 T14293 T14294 amod ng/ml,LPS
R11936 T14294 T14291 pobj LPS,with
R11937 T14295 T14298 punct (,C
R11938 T14296 T14298 nmod A,C
R11939 T14297 T14298 punct ",",C
R11940 T14298 T14294 appos C,LPS
R11941 T14299 T14298 punct ",",C
R11942 T14300 T14298 conj E,C
R11943 T14301 T14300 punct ",",E
R11944 T14302 T14300 conj G,E
R11945 T14303 T14300 punct ),E
R11946 T14304 T14298 cc or,C
R11947 T14305 T14298 nummod 25,C
R11948 T14306 T14309 nmod µg,poly
R13125 T15667 T15668 amod aforementioned,cytokines
R13126 T15668 T15665 pobj cytokines,of
R13127 T15669 T15671 punct (,shown
R13128 T15670 T15671 neg not,shown
R13129 T15671 T15664 parataxis shown,production
R13130 T15672 T15664 punct ),production
R13131 T15673 T15661 punct .,had
R13132 T15674 T15676 nsubjpass Results,analyzed
R13133 T15675 T15676 auxpass are,analyzed
R13134 T15676 T15676 ROOT analyzed,analyzed
R13135 T15677 T15676 prep based,analyzed
R13136 T15678 T15677 prep on,based
R13137 T15679 T15678 pobj 2,on
R13138 T15680 T15681 nummod 4,mice
R13139 T15681 T15679 appos mice,2
R13140 T15682 T15681 prep per,mice
R13141 T15683 T15682 pobj group,per
R11684 T13999 T13993 punct .,collected
R11685 T14000 T14001 nsubj Data,are
R11686 T14001 T14001 ROOT are,are
R11687 T14002 T14001 attr representative,are
R11688 T14003 T14002 prep of,representative
R11689 T14004 T14006 nummod three,experiments
R11690 T14005 T14006 amod independent,experiments
R11691 T14006 T14003 pobj experiments,of
R11914 T14272 T14288 nsubjpass WT,washed
R11915 T14273 T14272 cc and,WT
R11916 T14274 T14275 compound cpdm,BMDC
R11917 T14275 T14272 conj BMDC,WT
R11918 T14276 T14277 punct (,5
R11919 T14277 T14280 nummod 5,cells
R11920 T14278 T14280 nummod ×,cells
R11921 T14279 T14280 nummod 105,cells
R11922 T14280 T14272 appos cells,WT
R11923 T14281 T14280 prep in,cells
R11924 T14282 T14283 nummod 0.2,ml
R11925 T14283 T14285 nmod ml,medium
R11926 T14284 T14285 amod complete,medium
R11927 T14285 T14281 pobj medium,in
R11928 T14286 T14280 punct ),cells
R11929 T14287 T14288 auxpass were,washed
R11930 T14288 T14288 ROOT washed,washed
R11931 T14289 T14288 cc and,washed
R11932 T14290 T14288 conj stimulated,washed
R11933 T14291 T14290 prep with,stimulated
R11934 T14292 T14294 nummod 100,LPS
R11996 T14354 T14353 punct ",",C
R11997 T14355 T14353 appos D,C
R11998 T14356 T14353 punct ),C
R11999 T14357 T14353 punct ",",C
R12000 T14358 T14343 pobj Gmcsf,of
R12001 T14359 T14360 punct (,E
R12002 T14360 T14358 appos E,Gmcsf
R12003 T14361 T14358 punct ",",Gmcsf
R12004 T14362 T14358 appos F,Gmcsf
R12005 T14363 T14358 punct ),Gmcsf
R12006 T14364 T14336 punct ",",subject
R12007 T14365 T14336 cc and,subject
R12008 T14366 T14336 conj Ifnb,subject
R12009 T14367 T14368 punct (,G
R12010 T14368 T14372 nmod G,mRNA
R12011 T14369 T14368 punct ",",G
R12012 T14370 T14368 appos H,G
R12013 T14371 T14372 punct ),mRNA
R12014 T14372 T14334 conj mRNA,extracted
R12015 T14373 T14334 punct .,extracted
R12016 T14374 T14375 nsubj Bars,represent
R12017 T14375 T14375 ROOT represent,represent
R12018 T14376 T14378 amod mean,SD
R12019 T14377 T14378 compound ±,SD
R12020 T14378 T14375 dobj SD,represent
R12021 T14379 T14375 punct .,represent
R12022 T14380 T14381 nsubj Data,are
R12023 T14381 T14381 ROOT are,are
R12024 T14382 T14381 attr representative,are
R12025 T14383 T14382 prep of,representative
R12026 T14384 T14386 nummod two,experiments
R12027 T14385 T14386 amod independent,experiments
R12028 T14386 T14383 pobj experiments,of
R12029 T14387 T14381 punct .,are
R12188 T14584 T14588 amod Normal,expression

UBERON-AE

Id Subject Object Predicate Lexical cue
T2869 7867-7873 http://purl.obolibrary.org/obo/UBERON_0002106 denotes spleen
T2870 8072-8078 http://purl.obolibrary.org/obo/UBERON_0002106 denotes spleen
T2871 8246-8252 http://purl.obolibrary.org/obo/UBERON_0002106 denotes spleen
T6671 20554-20560 http://purl.obolibrary.org/obo/UBERON_0000062 denotes organs
T6672 20615-20620 http://purl.obolibrary.org/obo/UBERON_0000062 denotes organ
T6673 20565-20572 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissues
T6674 22637-22644 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissues
T6675 22695-22701 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissue
T6676 22781-22787 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissue
T6677 23422-23428 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissue
T6678 23479-23485 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissue
T6679 23117-23128 http://purl.obolibrary.org/obo/UBERON_0000353 denotes parenchymal
T6680 23370-23381 http://purl.obolibrary.org/obo/UBERON_0000353 denotes parenchymal
T6681 23148-23153 http://purl.obolibrary.org/obo/UBERON_0002107 denotes liver
T6682 23159-23168 http://purl.obolibrary.org/obo/UBERON_0000160 denotes intestine
T10421 28487-28492 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T10422 28963-28970 http://purl.obolibrary.org/obo/UBERON_0002106 denotes spleens
T10947 29748-29755 http://purl.obolibrary.org/obo/UBERON_0002106 denotes spleens
T12754 6610-6616 http://purl.obolibrary.org/obo/UBERON_0002106 denotes spleen
T13262 8366-8373 http://purl.obolibrary.org/obo/UBERON_0002106 denotes Spleens
T69 832-838 http://purl.obolibrary.org/obo/UBERON_0002106 denotes spleen
T70 916-927 http://purl.obolibrary.org/obo/UBERON_0002371 denotes bone marrow
T946 1862-1867 http://purl.obolibrary.org/obo/UBERON_0000955 denotes brain
T947 1934-1941 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissues
T948 2434-2440 http://purl.obolibrary.org/obo/UBERON_0000062 denotes organs
T949 3905-3911 http://purl.obolibrary.org/obo/UBERON_0000062 denotes organs
T2198 5448-5454 http://purl.obolibrary.org/obo/UBERON_0002106 denotes spleen
T2864 7444-7460 http://purl.obolibrary.org/obo/UBERON_0001744 denotes lymphoid tissues
T2865 9630-9645 http://purl.obolibrary.org/obo/UBERON_0001744 denotes lymphoid tissue
T2866 7453-7460 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissues
T2867 9639-9645 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissue
T2868 7468-7475 http://purl.obolibrary.org/obo/UBERON_0002106 denotes spleens

GO-BP

Id Subject Object Predicate Lexical cue
T699 25-44 http://purl.obolibrary.org/obo/GO_0001816 denotes Cytokine Production
T700 1521-1540 http://purl.obolibrary.org/obo/GO_0001816 denotes cytokine production
T701 313-325 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T702 1712-1724 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T703 401-410 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T704 1148-1157 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T705 453-461 http://purl.obolibrary.org/obo/GO_0007349 denotes cellular
T706 1130-1143 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T707 1253-1272 http://purl.obolibrary.org/obo/GO_0051092 denotes activation of NF-κB
T708 1253-1282 http://purl.obolibrary.org/obo/GO_1901224 denotes activation of NF-κB signaling
T709 1267-1282 http://purl.obolibrary.org/obo/GO_0038061 denotes NF-κB signaling
T710 1273-1282 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T711 1368-1377 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T712 1305-1309 http://purl.obolibrary.org/obo/GO_0004707 denotes ERK1
T713 1334-1337 http://purl.obolibrary.org/obo/GO_0004707 denotes p38
T714 1363-1367 http://purl.obolibrary.org/obo/GO_0008384 denotes TBK1
T715 1368-1386 http://purl.obolibrary.org/obo/GO_0007165 denotes signaling pathways
T716 1472-1491 http://purl.obolibrary.org/obo/GO_0042088 denotes Th1 immune response
T717 1476-1491 http://purl.obolibrary.org/obo/GO_0006955 denotes immune response
T718 1740-1755 http://purl.obolibrary.org/obo/GO_0006955 denotes immune response
T719 1476-1506 http://purl.obolibrary.org/obo/GO_0002922 denotes immune response but stimulated
T720 1476-1506 http://purl.obolibrary.org/obo/GO_0045089 denotes immune response but stimulated
T721 1476-1506 http://purl.obolibrary.org/obo/GO_0050778 denotes immune response but stimulated
T722 1476-1506 http://purl.obolibrary.org/obo/GO_0002821 denotes immune response but stimulated
T1993 2190-2204 http://purl.obolibrary.org/obo/GO_0009299 denotes mRNA synthesis
T1994 2195-2204 http://purl.obolibrary.org/obo/GO_0009058 denotes synthesis
T1995 2400-2411 http://purl.obolibrary.org/obo/GO_0032502 denotes development
T1996 2516-2519 http://purl.obolibrary.org/obo/GO_0007568 denotes age
T1997 2823-2852 http://purl.obolibrary.org/obo/GO_0001806 denotes delayed type hypersensitivity
T1998 2836-2861 http://purl.obolibrary.org/obo/GO_0002524 denotes hypersensitivity response
T1999 2876-2885 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T2000 4079-4088 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T2001 4143-4152 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T2002 2922-2942 http://purl.obolibrary.org/obo/GO_0042088 denotes Th1 immune responses
T2003 2926-2942 http://purl.obolibrary.org/obo/GO_0006955 denotes immune responses
T2004 3940-3956 http://purl.obolibrary.org/obo/GO_0006955 denotes immune responses
T2005 4040-4055 http://purl.obolibrary.org/obo/GO_0006955 denotes immune response
T2006 2964-2983 http://purl.obolibrary.org/obo/GO_0042092 denotes Th2 immune response
T2007 2964-2983 http://purl.obolibrary.org/obo/GO_0002828 denotes Th2 immune response
T2008 3474-3483 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T2009 3740-3747 http://purl.obolibrary.org/obo/GO_0023052 denotes signals
T2010 3549-3559 http://purl.obolibrary.org/obo/GO_0046960 denotes sensitized
T2011 3576-3585 http://purl.obolibrary.org/obo/GO_0006915 denotes apoptosis
T2012 3576-3585 http://purl.obolibrary.org/obo/GO_0097194 denotes apoptosis
T2013 3590-3600 http://purl.obolibrary.org/obo/GO_0008219 denotes cell death
T2014 3595-3600 http://purl.obolibrary.org/obo/GO_0016265 denotes death
T2015 3790-3814 http://purl.obolibrary.org/obo/GO_0002820 denotes adaptive immune response
T2016 3790-3814 http://purl.obolibrary.org/obo/GO_0002821 denotes adaptive immune response
T2017 3790-3814 http://purl.obolibrary.org/obo/GO_0002819 denotes adaptive immune response
T2018 3790-3814 http://purl.obolibrary.org/obo/GO_0002250 denotes adaptive immune response
T2019 3855-3874 http://purl.obolibrary.org/obo/GO_0019882 denotes processed antigenic
T2020 3855-3883 http://purl.obolibrary.org/obo/GO_0048002 denotes processed antigenic peptides
T2021 3924-3939 http://purl.obolibrary.org/obo/GO_0001913 denotes T-cell-mediated
T2022 3926-3946 http://purl.obolibrary.org/obo/GO_0002456 denotes cell-mediated immune
T2023 3924-3946 http://purl.obolibrary.org/obo/GO_0002709 denotes T-cell-mediated immune
T2024 3926-3946 http://purl.obolibrary.org/obo/GO_0002449 denotes cell-mediated immune
T2025 3926-3946 http://purl.obolibrary.org/obo/GO_0019724 denotes cell-mediated immune
T2026 3926-3946 http://purl.obolibrary.org/obo/GO_0002228 denotes cell-mediated immune
T2027 3926-3956 http://purl.obolibrary.org/obo/GO_0002443 denotes cell-mediated immune responses
T2028 3926-3946 http://purl.obolibrary.org/obo/GO_0001909 denotes cell-mediated immune
T2029 4079-4101 http://purl.obolibrary.org/obo/GO_0050663 denotes secretion of cytokines
T2030 4134-4152 http://purl.obolibrary.org/obo/GO_0050663 denotes cytokine secretion
T2031 4379-4395 http://purl.obolibrary.org/obo/GO_0051092 denotes NF-κB activation
T2032 4463-4482 http://purl.obolibrary.org/obo/GO_0001816 denotes cytokine production
T2708 5419-5431 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T2709 5490-5503 http://purl.obolibrary.org/obo/GO_0030097 denotes hematopoiesis
T2710 6005-6017 http://purl.obolibrary.org/obo/GO_0051179 denotes localization
T3645 7429-7440 http://purl.obolibrary.org/obo/GO_0032502 denotes development
T3646 9941-9950 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T3647 9941-9959 http://purl.obolibrary.org/obo/GO_0007165 denotes signaling pathways
T4209 10438-10447 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T5376 12851-12870 http://purl.obolibrary.org/obo/GO_0001816 denotes cytokine production
T5377 14219-14238 http://purl.obolibrary.org/obo/GO_0001816 denotes cytokine production
T5378 15283-15302 http://purl.obolibrary.org/obo/GO_0001816 denotes cytokine production
T5379 12930-12939 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T5380 13263-13272 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T5381 13984-13993 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T5382 15113-15122 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T5383 15247-15256 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T5384 15433-15442 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T5385 16022-16031 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T5386 13002-13020 http://purl.obolibrary.org/obo/GO_0050663 denotes cytokine secretion
T5387 14029-14047 http://purl.obolibrary.org/obo/GO_0050663 denotes cytokine secretion
T5388 13002-13034 http://purl.obolibrary.org/obo/GO_0050715 denotes cytokine secretion in stimulated
T5389 13011-13020 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T5390 13604-13613 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T5391 13670-13679 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T5392 13897-13906 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T5393 14038-14047 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T5394 13011-13034 http://purl.obolibrary.org/obo/GO_0051047 denotes secretion in stimulated
T5395 13665-13679 http://purl.obolibrary.org/obo/GO_0072610 denotes IL12 secretion
T5396 15018-15031 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T5397 15554-15567 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T5398 15104-15112 http://purl.obolibrary.org/obo/GO_0007349 denotes cellular
T5399 15113-15131 http://purl.obolibrary.org/obo/GO_0007165 denotes signaling pathways
T5400 15154-15158 http://purl.obolibrary.org/obo/GO_0008384 denotes TBK1
T5401 15224-15228 http://purl.obolibrary.org/obo/GO_0008384 denotes TBK1
T5402 15818-15822 http://purl.obolibrary.org/obo/GO_0008384 denotes TBK1
T5403 15995-15999 http://purl.obolibrary.org/obo/GO_0008384 denotes TBK1
T5404 15169-15173 http://purl.obolibrary.org/obo/GO_0004707 denotes MAPK
T5405 15242-15246 http://purl.obolibrary.org/obo/GO_0004707 denotes MAPK
T5406 15824-15828 http://purl.obolibrary.org/obo/GO_0004707 denotes ERK1
T5407 15829-15839 http://purl.obolibrary.org/obo/GO_0004707 denotes 2, and p38
T5408 15836-15839 http://purl.obolibrary.org/obo/GO_0004707 denotes p38
T5409 16018-16021 http://purl.obolibrary.org/obo/GO_0004707 denotes p38
T5410 15242-15256 http://purl.obolibrary.org/obo/GO_0000165 denotes MAPK signaling
T5411 15360-15375 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T5412 15453-15468 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T5413 15840-15855 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T5414 15360-15386 http://purl.obolibrary.org/obo/GO_0007252 denotes phosphorylation of the IκB
T5415 15512-15527 http://purl.obolibrary.org/obo/GO_0007252 denotes NF-κB inhibitor
T5416 15493-15504 http://purl.obolibrary.org/obo/GO_0009056 denotes degradation
T5417 15512-15527 http://purl.obolibrary.org/obo/GO_0032088 denotes NF-κB inhibitor
T5418 15554-15592 http://purl.obolibrary.org/obo/GO_0042991 denotes transcription factors into the nucleus
T5419 15959-15975 http://purl.obolibrary.org/obo/GO_0051092 denotes NF-κB activation
T6453 16915-16935 http://purl.obolibrary.org/obo/GO_0030154 denotes cell differentiation
T6454 18159-18168 http://purl.obolibrary.org/obo/GO_0031295 denotes T cell co
T9123 19502-19513 http://purl.obolibrary.org/obo/GO_0032502 denotes development
T9124 20121-20132 http://purl.obolibrary.org/obo/GO_0032502 denotes development
T9125 21546-21557 http://purl.obolibrary.org/obo/GO_0032502 denotes development
T9126 23946-23957 http://purl.obolibrary.org/obo/GO_0032502 denotes development
T9127 19675-19694 http://purl.obolibrary.org/obo/GO_0001816 denotes cytokine production
T9128 19820-19829 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T9129 22023-22032 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T9130 22355-22364 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T9131 22814-22823 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T9132 23870-23879 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T9133 24781-24790 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T9134 24942-24951 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T9135 25130-25139 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T9136 25329-25338 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T9137 25400-25409 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T9138 25677-25686 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T9139 20249-20261 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T9140 20500-20512 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T9141 21712-21724 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T9142 22956-22968 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T9143 23188-23200 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T9144 20457-20478 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammatory response
T9145 20763-20773 http://purl.obolibrary.org/obo/GO_0008219 denotes cell death
T9146 20957-20967 http://purl.obolibrary.org/obo/GO_0008219 denotes cell death
T9147 20768-20773 http://purl.obolibrary.org/obo/GO_0016265 denotes death
T9148 20962-20967 http://purl.obolibrary.org/obo/GO_0016265 denotes death
T9149 20957-20975 http://purl.obolibrary.org/obo/GO_0043276 denotes cell death-induced
T9150 20957-20975 http://purl.obolibrary.org/obo/GO_0071888 denotes cell death-induced
T9151 20998-21007 http://purl.obolibrary.org/obo/GO_0006915 denotes Apoptosis
T9152 20998-21007 http://purl.obolibrary.org/obo/GO_0097194 denotes Apoptosis
T9153 20998-21024 http://purl.obolibrary.org/obo/GO_0097283 denotes Apoptosis of keratinocytes
T9154 21427-21446 http://purl.obolibrary.org/obo/GO_0042088 denotes Th1 immune response
T9155 21431-21446 http://purl.obolibrary.org/obo/GO_0006955 denotes immune response
T9156 21839-21855 http://purl.obolibrary.org/obo/GO_0006955 denotes immune responses
T9157 21835-21855 http://purl.obolibrary.org/obo/GO_0042092 denotes Th2 immune responses
T9158 21835-21855 http://purl.obolibrary.org/obo/GO_0002828 denotes Th2 immune responses
T9159 22018-22022 http://purl.obolibrary.org/obo/GO_0004707 denotes MAPK
T9160 22299-22303 http://purl.obolibrary.org/obo/GO_0004707 denotes ERK1
T9161 22310-22313 http://purl.obolibrary.org/obo/GO_0004707 denotes p38
T9162 22018-22032 http://purl.obolibrary.org/obo/GO_0000165 denotes MAPK signaling
T9163 22161-22177 http://purl.obolibrary.org/obo/GO_0051092 denotes NF-κB activation
T9164 22509-22525 http://purl.obolibrary.org/obo/GO_0051092 denotes NF-κB activation
T9165 23097-23113 http://purl.obolibrary.org/obo/GO_0051092 denotes NF-κB activation
T9166 23615-23631 http://purl.obolibrary.org/obo/GO_0051092 denotes NF-κB activation
T9167 24293-24309 http://purl.obolibrary.org/obo/GO_0051092 denotes NF-κB activation
T9168 24561-24577 http://purl.obolibrary.org/obo/GO_0051092 denotes NF-κB activation
T9169 24811-24827 http://purl.obolibrary.org/obo/GO_0051092 denotes NF-κB activation
T9170 25212-25228 http://purl.obolibrary.org/obo/GO_0051092 denotes NF-κB activation
T9171 25515-25531 http://purl.obolibrary.org/obo/GO_0051092 denotes NF-κB activation
T9172 22288-22292 http://purl.obolibrary.org/obo/GO_0008384 denotes TBK1
T9173 22534-22547 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T9174 23422-23440 http://purl.obolibrary.org/obo/GO_0001894 denotes tissue homeostasis
T9175 23429-23440 http://purl.obolibrary.org/obo/GO_0042592 denotes homeostasis
T9176 24200-24211 http://purl.obolibrary.org/obo/GO_0042592 denotes homeostasis
T9177 25890-25901 http://purl.obolibrary.org/obo/GO_0042592 denotes homeostasis
T9178 23870-23887 http://purl.obolibrary.org/obo/GO_0007165 denotes signaling pathway
T9179 25329-25346 http://purl.obolibrary.org/obo/GO_0007165 denotes signaling pathway
T9180 25400-25418 http://purl.obolibrary.org/obo/GO_0007165 denotes signaling cascades
T9181 24183-24193 http://purl.obolibrary.org/obo/GO_0065007 denotes regulation
T9182 25449-25459 http://purl.obolibrary.org/obo/GO_0065007 denotes regulation
T9183 24197-24211 http://purl.obolibrary.org/obo/GO_0036145 denotes DC homeostasis
T9184 25105-25120 http://purl.obolibrary.org/obo/GO_0016874 denotes ligase activity
T9185 25430-25459 http://purl.obolibrary.org/obo/GO_2000395 denotes ubiquitin-mediated regulation
T9186 26031-26049 http://purl.obolibrary.org/obo/GO_0050663 denotes cytokine secretion
T9187 26040-26049 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T9932 26704-26707 http://purl.obolibrary.org/obo/GO_0007568 denotes age
T11254 29926-29935 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T11843 30627-30648 http://purl.obolibrary.org/obo/GO_0001171 denotes Reverse transcription
T11844 30971-30992 http://purl.obolibrary.org/obo/GO_0001171 denotes Reverse transcription
T11845 30635-30648 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T11846 30979-30992 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T11847 30870-30883 http://purl.obolibrary.org/obo/GO_0003899 denotes Transcriptase
T11848 30870-30883 http://purl.obolibrary.org/obo/GO_0003968 denotes Transcriptase
T11849 30870-30883 http://purl.obolibrary.org/obo/GO_0034062 denotes Transcriptase
T11850 31147-31162 http://purl.obolibrary.org/obo/GO_0010467 denotes Gene Expression
T11851 31388-31403 http://purl.obolibrary.org/obo/GO_0010467 denotes gene expression
T11852 31204-31206 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T12075 31459-31477 http://purl.obolibrary.org/obo/GO_0050663 denotes cytokine secretion
T12076 31468-31477 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T12628 32823-32827 http://purl.obolibrary.org/obo/GO_0008384 denotes TBK1
T12629 32838-32841 http://purl.obolibrary.org/obo/GO_0004707 denotes p38
T12630 32851-32854 http://purl.obolibrary.org/obo/GO_0004707 denotes p38
T12631 32866-32870 http://purl.obolibrary.org/obo/GO_0004707 denotes ERK1
T12632 32886-32890 http://purl.obolibrary.org/obo/GO_0004707 denotes ERK1
T13211 6501-6513 http://purl.obolibrary.org/obo/GO_0006954 denotes inflammation
T13212 6563-6576 http://purl.obolibrary.org/obo/GO_0030097 denotes hematopoiesis
T13213 7217-7226 http://purl.obolibrary.org/obo/GO_0051179 denotes localized
T13777 8447-8456 http://purl.obolibrary.org/obo/GO_0007586 denotes digestion
T14210 11721-11737 http://purl.obolibrary.org/obo/GO_0051410 denotes nitric oxide (NO
T14904 14334-14343 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion
T15263 16105-16114 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T15264 16420-16429 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T15265 16393-16397 http://purl.obolibrary.org/obo/GO_0008384 denotes TBK1
T15266 16404-16408 http://purl.obolibrary.org/obo/GO_0004707 denotes ERK1
T15267 16416-16419 http://purl.obolibrary.org/obo/GO_0004707 denotes p38
T15268 16420-16438 http://purl.obolibrary.org/obo/GO_0007165 denotes signaling pathways
T15269 16420-16444 http://purl.obolibrary.org/obo/GO_0035791 denotes signaling pathways. Beta
T15270 16484-16492 http://purl.obolibrary.org/obo/GO_0007349 denotes Cellular
T15697 18532-18551 http://purl.obolibrary.org/obo/GO_0001816 denotes cytokine production
T15698 18959-18968 http://purl.obolibrary.org/obo/GO_0046903 denotes secretion

GO-MF

Id Subject Object Predicate Lexical cue
T724 1305-1309 http://purl.obolibrary.org/obo/GO_0004707 denotes ERK1
T725 1334-1337 http://purl.obolibrary.org/obo/GO_0004707 denotes p38
T726 1363-1367 http://purl.obolibrary.org/obo/GO_0008384 denotes TBK1
T2038 3113-3121 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T2712 4982-4991 http://purl.obolibrary.org/obo/GO_0031386 denotes ubiquitin
T2713 5029-5048 http://purl.obolibrary.org/obo/GO_0008536 denotes Ran-binding protein
T2714 5033-5040 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T2715 5033-5048 http://purl.obolibrary.org/obo/GO_0005515 denotes binding protein
T2716 5033-5050 http://purl.obolibrary.org/obo/GO_0051721 denotes binding protein 2
T3648 9861-9867 http://purl.obolibrary.org/obo/GO_0005488 denotes ligand
T3649 9890-9896 http://purl.obolibrary.org/obo/GO_0005488 denotes ligand
T5420 15154-15158 http://purl.obolibrary.org/obo/GO_0008384 denotes TBK1
T5421 15224-15228 http://purl.obolibrary.org/obo/GO_0008384 denotes TBK1
T5422 15818-15822 http://purl.obolibrary.org/obo/GO_0008384 denotes TBK1
T5423 15995-15999 http://purl.obolibrary.org/obo/GO_0008384 denotes TBK1
T5424 15169-15173 http://purl.obolibrary.org/obo/GO_0004707 denotes MAPK
T5425 15242-15246 http://purl.obolibrary.org/obo/GO_0004707 denotes MAPK
T5426 15824-15828 http://purl.obolibrary.org/obo/GO_0004707 denotes ERK1
T5427 15829-15839 http://purl.obolibrary.org/obo/GO_0004707 denotes 2, and p38
T5428 15836-15839 http://purl.obolibrary.org/obo/GO_0004707 denotes p38
T5429 16018-16021 http://purl.obolibrary.org/obo/GO_0004707 denotes p38
T6455 17299-17305 http://purl.obolibrary.org/obo/GO_0005488 denotes ligand
T9190 22018-22022 http://purl.obolibrary.org/obo/GO_0004707 denotes MAPK
T9191 22299-22303 http://purl.obolibrary.org/obo/GO_0004707 denotes ERK1
T9192 22310-22313 http://purl.obolibrary.org/obo/GO_0004707 denotes p38
T9193 22288-22292 http://purl.obolibrary.org/obo/GO_0008384 denotes TBK1
T9194 24388-24395 http://purl.obolibrary.org/obo/GO_0005488 denotes ligands
T9195 25089-25098 http://purl.obolibrary.org/obo/GO_0031386 denotes ubiquitin
T9196 25430-25439 http://purl.obolibrary.org/obo/GO_0031386 denotes ubiquitin
T9197 25105-25120 http://purl.obolibrary.org/obo/GO_0016874 denotes ligase activity
T11853 30870-30883 http://purl.obolibrary.org/obo/GO_0003899 denotes Transcriptase
T11854 30870-30883 http://purl.obolibrary.org/obo/GO_0003968 denotes Transcriptase
T11855 30870-30883 http://purl.obolibrary.org/obo/GO_0034062 denotes Transcriptase
T11856 31204-31206 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T12338 32154-32161 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T12339 32233-32241 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T12340 32394-32402 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T12633 32576-32583 http://purl.obolibrary.org/obo/GO_0005488 denotes ligands
T12634 32727-32737 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T12635 32823-32827 http://purl.obolibrary.org/obo/GO_0008384 denotes TBK1
T12636 32838-32841 http://purl.obolibrary.org/obo/GO_0004707 denotes p38
T12637 32851-32854 http://purl.obolibrary.org/obo/GO_0004707 denotes p38
T12638 32866-32870 http://purl.obolibrary.org/obo/GO_0004707 denotes ERK1
T12639 32886-32890 http://purl.obolibrary.org/obo/GO_0004707 denotes ERK1
T13214 6238-6247 http://purl.obolibrary.org/obo/GO_0031386 denotes ubiquitin
T13215 7105-7113 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T13778 8685-8695 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T15271 16346-16356 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T15272 16393-16397 http://purl.obolibrary.org/obo/GO_0008384 denotes TBK1
T15273 16404-16408 http://purl.obolibrary.org/obo/GO_0004707 denotes ERK1
T15274 16416-16419 http://purl.obolibrary.org/obo/GO_0004707 denotes p38
T9188 21309-21338 http://purl.obolibrary.org/obo/GO_0038187 denotes pattern recognition receptors
T2039 3389-3398 http://purl.obolibrary.org/obo/GO_0031386 denotes ubiquitin
T2040 4401-4407 http://purl.obolibrary.org/obo/GO_0005488 denotes ligand

GO-CC

Id Subject Object Predicate Lexical cue
T727 552-557 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T728 946-951 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T729 1570-1575 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T4210 10723-10728 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5432 13097-13113 http://purl.obolibrary.org/obo/GO_0043235 denotes receptor complex
T5433 13333-13349 http://purl.obolibrary.org/obo/GO_0043235 denotes receptor complex
T5434 13097-13116 http://purl.obolibrary.org/obo/GO_0005893 denotes receptor complex, 2
T5435 15473-15483 http://purl.obolibrary.org/obo/GO_0000502 denotes proteasome
T5436 15585-15592 http://purl.obolibrary.org/obo/GO_0005634 denotes nucleus
T5437 15871-15876 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6456 16915-16919 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T6457 17128-17132 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T6458 18161-18165 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T6459 18364-18369 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T9933 26803-26813 http://purl.obolibrary.org/obo/GO_0005694 denotes chromosome
T10299 27360-27363 http://purl.obolibrary.org/obo/GO_0030896 denotes CCC
T10300 27787-27792 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10301 27859-27864 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10419 28211-28215 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T10420 28234-28239 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10919 28414-28419 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10920 28821-28826 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10921 29221-29226 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10922 29378-29383 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10923 29470-29475 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T10924 29140-29143 http://purl.obolibrary.org/obo/GO_0005680 denotes APC
T11255 29518-29522 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T11256 29657-29662 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T11257 29723-29728 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T11258 30067-30072 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T11259 30095-30100 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T11857 30263-30268 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12077 31572-31577 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12078 31630-31635 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12341 31899-31904 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12342 31958-31963 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12343 32095-32100 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12344 32169-32174 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12345 32233-32241 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T12346 32394-32402 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T12347 32233-32241 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T12348 32394-32402 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T12640 32727-32737 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T12641 32727-32737 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T13216 6950-6955 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13217 7032-7037 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13218 7166-7170 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T13219 7105-7113 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T13220 7105-7113 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T13221 7207-7216 http://purl.obolibrary.org/obo/GO_0005737 denotes cytoplasm
T13779 8685-8695 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T13780 8685-8695 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T13781 8841-8846 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13782 8911-8916 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13783 9052-9057 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13784 9204-9209 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13785 9292-9297 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13786 9463-9468 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13787 9480-9485 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14211 11507-11512 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14212 11788-11793 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14213 12128-12133 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14905 14530-14535 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14906 14601-14606 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14907 14807-14812 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15275 16153-16158 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15276 16287-16291 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T15277 16346-16356 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T15278 16346-16356 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T15699 18570-18575 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15700 18601-18606 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15701 18752-18757 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15702 18839-18844 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15703 18854-18859 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15704 19098-19103 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15705 19126-19131 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3650 7800-7805 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3651 7941-7946 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3652 8079-8084 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5430 13093-13113 http://purl.obolibrary.org/obo/GO_0046696 denotes LPS receptor complex
T5431 13329-13349 http://purl.obolibrary.org/obo/GO_0046696 denotes LPS receptor complex
T9198 19875-19879 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T9199 20763-20767 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T9200 20957-20961 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T9201 22685-22689 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T9202 23469-23473 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T9203 24084-24089 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T9204 24635-24640 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2041 1808-1831 http://purl.obolibrary.org/obo/GO_0014069 denotes post-synaptic densities
T2042 1835-1854 http://purl.obolibrary.org/obo/GO_0060076 denotes excitatory synapses
T2043 1846-1854 http://purl.obolibrary.org/obo/GO_0045202 denotes synapses
T2044 3113-3121 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T2045 3113-3121 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T2046 3414-3428 http://purl.obolibrary.org/obo/GO_0071797 denotes complex (LUBAC
T2047 3590-3594 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T2048 3926-3930 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T2049 4011-4016 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T2050 4504-4509 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14513 12481-12486 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

sentences

Id Subject Object Predicate Lexical cue
T107 0-118 Sentence denotes SHARPIN Is Essential for Cytokine Production, NF-κB Signaling, and Induction of Th1 Differentiation by Dendritic Cells
T108 165-265 Sentence denotes Spontaneous mutations of the Sharpin (SHANK-associated RH domain-interacting protein, other aliases:
T109 266-448 Sentence denotes Rbckl1, Sipl1) gene in mice result in systemic inflammation that is characterized by chronic proliferative dermatitis and dysregulated secretion of T helper1 (Th1) and Th2 cytokines.
T110 449-541 Sentence denotes The cellular and molecular mechanisms underlying this inflammatory phenotype remain elusive.
T111 542-712 Sentence denotes Dendritic cells may contribute to the initiation and progression of the phenotype of SHARPIN-deficient mice because of their pivotal role in innate and adaptive immunity.
T112 713-839 Sentence denotes Here we show by flow cytometry that SHARPIN- deficiency did not alter the distribution of different DC subtypes in the spleen.
T113 840-1074 Sentence denotes In response to TOLL-like receptor (TLR) agonists LPS and poly I:C, cultured bone marrow-derived dendritic cells (BMDC) from WT and mutant mice exhibited similar increases in expression of co-stimulatory molecules CD40, CD80, and CD86.
T114 1075-1226 Sentence denotes However, stimulated SHARPIN-deficient BMDC had reduced transcription and secretion of pro-inflammatory mediators IL6, IL12P70, GMCSF, and nitric oxide.
T115 1227-1399 Sentence denotes Mutant BMDC had defective activation of NF-κB signaling, whereas the MAPK1/3 (ERK1/2) and MAPK11/12/13/14 (p38 MAP kinase isoforms) and TBK1 signaling pathways were intact.
T116 1400-1576 Sentence denotes A mixed lymphocyte reaction showed that mutant BMDC only induced a weak Th1 immune response but stimulated increased Th2 cytokine production from allogeneic naïve CD4+ T cells.
T117 1577-1756 Sentence denotes In conclusion, loss of Sharpin in mice significantly affects the immune function of DC and this may partially account for the systemic inflammation and Th2-biased immune response.
T978 1771-1946 Sentence denotes SHARPIN was originally identified in post-synaptic densities of excitatory synapses in the brain of rats [1], but this protein is widely expressed in a variety of tissues [2].
T979 1947-2253 Sentence denotes Two allelic, autosomal recessive mutations in the Sharpin gene occurred spontaneously in two inbred strains of mice, C57B/KaLawRij-Sharpincpdm/Sharpincpdm and CBy.Ocb3/Dem-Sharpincpdm-Dem/Sharpincpdm-Dem, resulting in premature termination of mRNA synthesis and absence of a functional protein product [2].
T980 2254-2449 Sentence denotes Despite different genetic backgrounds, both mutations cause similar inflammatory disease with severe chronic progressive dermatitis and defective development of secondary lymphoid organs [2]–[4].
T981 2450-2520 Sentence denotes The dermatitis becomes clinically apparent at about four weeks of age.
T982 2521-2796 Sentence denotes There are accumulations of eosinophils, neutrophils and macrophages in the skin of Sharpincpdm/Sharpincpdm mutant mice (hereafter referred to as cpdm mice) associated with increased expression of Th2 cytokines in the skin and in the supernatants of activated splenocytes [5].
T983 2797-2984 Sentence denotes The mice have an impaired delayed type hypersensitivity response and decreased secretion of IFNγ [5], indicating a defect in Th1 immune responses and a bias towards a Th2 immune response.
T984 2985-3087 Sentence denotes Systemic treatment of cpdm mice with recombinant IL12 caused complete remission of the dermatitis [5].
T985 3088-3294 Sentence denotes Neutralization of IL5 by antibody treatment or crosses with IL5-deficient mice reduced the number of circulating and cutaneous eosinophils, but failed to reduce the onset and severity of the dermatitis [6].
T986 3295-3492 Sentence denotes Recently, three independent groups identified SHARPIN as an essential component of the linear ubiquitin chain assembly complex (LUBAC) that regulates TNFα-induced canonical NF-κB signaling [7]–[9].
T987 3493-3667 Sentence denotes SHARPIN-deficient mouse embryonic fibroblast (MEF) were sensitized to TNFα-induced apoptosis and cell death was implicated as a factor in the dermatitis of cpdm mice [7]–[9].
T988 3668-3820 Sentence denotes Dendritic cells (DC) have a sentinel role in sensing pathogen or danger signals and initiate and direct activation of the adaptive immune response [10].
T989 3821-3970 Sentence denotes Activated and mature DC can carry processed antigenic peptides, migrate to lymphoid organs, and induce T-cell-mediated immune responses or tolerance.
T990 3971-4102 Sentence denotes DC direct the differentiation of CD4+ T cells, and hence the type of immune response, through the selective secretion of cytokines.
T991 4103-4238 Sentence denotes We hypothesized that defective cytokine secretion by DC contributed to the Th2-biased inflammatory phenotype in SHARPIN-deficient mice.
T992 4239-4420 Sentence denotes The studies reported here found that lack of SHARPIN protein in BMDC caused defective expression of pro-inflammatory mediators and impaired NF-κB activation upon ligand stimulation.
T993 4421-4526 Sentence denotes The ability of cpdm BMDC to stimulate Th1 cytokine production in allogeneic CD4+ T cells was compromised.
T994 4527-4724 Sentence denotes Taken together, these results reveal that SHARPIN is a novel regulatory molecule in DC biology and suggest that the dysregulated function of SHARPIN-deficient DC plays a role in the cpdm phenotype.
T2877 7857-8132 Sentence denotes Since the spleen of cpdm mice is markedly enlarged and contains three times as many cells (Fig. 1C), the different percentages of splenic cDC and pDC between WT and mutant mice reflect the increased number of total spleen cells rather than a reduction in cDC and pDC numbers.
T2878 8133-8253 Sentence denotes These data indicate that the Sharpin mutation does not affect the distribution of the examined DC subsets in the spleen.
T2879 9576-9697 Sentence denotes BMDC from in vitro cultures functionally resemble non-lymphoid tissue DC and monocyte-derived inflammatory DC [15], [16].
T2880 9698-9752 Sentence denotes The yields of BMDC from WT and cpdm mice were similar.
T2881 9753-9851 Sentence denotes BMDC were CD11c+ and MHC II+ with low expression of co-stimulatory molecules CD40, CD80, and CD86.
T2882 9852-10009 Sentence denotes The TLR3 ligand poly I:C and the TLR4 ligand LPS each activate overlapping but different signaling pathways and were used to induce DC maturation [17], [18].
T2883 10010-10232 Sentence denotes Incubation with the TLR agonists for 24 hours resulted in increased expression of CD40, CD80, and CD86 on BMDC; however, there was no difference in the expression levels of these markers between WT and cpdm BMDC (Fig. 2B).
T2884 10233-10327 Sentence denotes Thus, SHARPIN deficiency did not influence the expression of co-stimulatory molecules by BMDC.
T3823 10329-10393 Sentence denotes Production of proinflammatory mediators by cpdm BMDC is impaired
T3824 10394-10622 Sentence denotes Incubation with LPS or poly I:C resulted in secretion of IL6, IL12P70, and GMCSF from both WT and cpdm BMDC; however, BMDC from cpdm mice produced significantly less of all three cytokines compared with WT BMDC (Fig. 3A–C, E–F).
T3825 10623-10838 Sentence denotes The amount of nitric oxide generated by mutant BMDC was also significantly reduced compared with WT cells (Fig. 3D), indicating severely disrupted production of proinflammatory mediators from Sharpin-deficient BMDC.
T3826 10839-11129 Sentence denotes In vivo complementation with a Sharpin gene-containing BAC reversed the phenotype of the mutant mice [2], and BMDC generated from these rescued mice secreted significantly more IL12P70 than BMDC from cpdm mice (Fig. 3G), supporting a necessary role of SHARPIN for the production of IL12P70.
T3827 11130-11345 Sentence denotes In addition, the transcript levels of the inflammatory cytokines Il6, Il12p40, Gmcsf, and Ifnb were examined, and these were all significantly reduced in stimulated cpdm BMDC when compared with WT controls (Fig. 4).
T4475 12842-12939 Sentence denotes Impaired cytokine production by cpdm BMDC is correlated with selective defects in NF-κB signaling
T4476 12940-13284 Sentence denotes There are a number of possible explanations for the defective cytokine secretion in stimulated cpdm BMDC, including 1) reduced surface expression of the LPS receptor complex, 2) increased production of anti-inflammatory mediators, 3) increased expression of negative regulators of TLR pathways, and 4) impaired TLR-induced signaling activation.
T4477 13285-13452 Sentence denotes We determined the surface expression of the LPS receptor complex that comprises TLR4, the accessory proteins CD14 and myeloid differentiation factor 2 (MD2/LY96) [19].
T4478 13453-13580 Sentence denotes Flow cytometric analysis shows that the expression levels of CD14 and TLR4/MD2 between WT and cpdm BMDC were similar (Fig. 5A).
T4479 13581-13714 Sentence denotes We then quantified the secretion of the suppressive cytokines IL10 that can inhibit IL12 secretion in an autocrine manner [20], [21].
T4480 13715-13931 Sentence denotes The supernatants from LPS-stimulated cpdm BMDC contained significantly lower levels of IL10 than stimulated WT BMDC (Fig. 5B), suggesting that IL10 was not responsible for decreased secretion of IL12P70 by cpdm BMDC.
T4481 13932-14048 Sentence denotes Increased expression of a negative regulator of TLR signaling such as A20 [22] may also suppress cytokine secretion.
T4482 14049-14239 Sentence denotes However, the transcript level of A20 was lower in LPS-activated cpdm BMDC than WT controls (Fig. 5C), thereby ruling out overexpression of A20 as a factor in the reduced cytokine production.
T4483 15014-15184 Sentence denotes The transcription of TLR3/4-induced proinflammatory intermediates is tightly regulated by cellular signaling pathways, in particular NF-κB, TBK1/IRF3, and MAPK [23]–[27].
T4484 15185-15342 Sentence denotes We next determined if disrupted NF-κB, TBK1/IRF3, and/or MAPK signaling may underlie the impaired cytokine production from stimulated Sharpin-deficient BMDC.
T4485 15343-15593 Sentence denotes Stimulus-induced phosphorylation of the IκB kinase (IKK1/2) is an essential step in NF-κB signaling, allowing phosphorylation and proteasome-mediated degradation of the NF-κB inhibitor IκBα to release the NF-κB transcription factors into the nucleus.
T4486 15594-15775 Sentence denotes The amount of phosphorylated IKK1/2 (p-IKK1/2) and IκBα (p-IκBα) following incubation with LPS or poly I:C was severely decreased in cpdm BMDC as compared with WT controls (Fig. 6).
T4487 15776-15886 Sentence denotes The cpdm BMDC exhibited similar levels of TBK1, ERK1/2, and p38 phosphorylation to those of WT cells (Fig. 6).
T4488 15887-16040 Sentence denotes These results indicate that the absence of functional SHARPIN decreased NF-κB activation but did not affect TBK1/IRF3, ERK1/2, and p38 signaling in BMDC.
T5820 16693-16742 Sentence denotes Th2-biased immunogenicity of stimulated cpdm BMDC
T5821 16743-16956 Sentence denotes The defective IL12 production (Fig. 3A) and Th2-dominant cytokine profile in cpdm mice [5] suggest that the absence of SHARPIN affects the ability of cpdm BMDC to induce T cell differentiation into effector cells.
T5822 16957-17257 Sentence denotes Co-culture of allogeneic naïve CD4+ T cells with WT BMDC stimulated with LPS or poly I:C elicited robust IFNγ production, whereas the concentration of IFNγ in cpdm BMDC-T cell cultures was significantly lower after LPS stimulation (Fig. 7A), indicating impaired Th1-polarizing abilities of cpdm BMDC.
T5823 17258-17394 Sentence denotes In addition to TLR3/4 agonists, the TLR2 ligand Pam3CYS was used since it has been shown to induce both Th1 and Th2 responses [28]–[30].
T5824 17395-17507 Sentence denotes Pam3CYS-matured WT BMDC induced robust IFNγ production at a significantly higher level than cpdm BMDC (Fig. 7A).
T5825 17508-17688 Sentence denotes The reduced Th1 differentiation following Pam3CYS stimulation is consistent with the recent report of decreased IL12 production in cpdm macrophages following TLR2 stimulation [31].
T5826 17689-17856 Sentence denotes In contrast, more Th2-specific IL4 cytokine was produced in the cpdm BMDC co-cultures than the WT control (Fig. 7B), suggesting Th2-skewed immunogenicity of cpdm BMDC.
T5827 17857-18025 Sentence denotes The production of Th17-specific cytokine IL17A following LPS stimulation of dendritic cells was similar between stimulated WT and cpdm BMDC cocultures (data not shown).
T5828 18026-18266 Sentence denotes Despite the distinct Th1- and Th2-stimulating abilities of WT and cpdm BMDC, they were equally effective in IL2 production from BMDC-T cell co-cultures except for poly I:C stimulation where WT BMDC induced more IL2 than cpdm BMDC (Fig. 7C).
T5829 18267-18447 Sentence denotes The Th2-biased stimulating capability of cpdm BMDC when co-cultured with allogeneic naïve CD4+ T cells is consistent with the Th2-dominant cytokine phenotype observed in cpdm mice.
T6766 19355-19536 Sentence denotes The present report showed that null mutations of the mouse Sharpin gene did not affect the steady-state distribution of splenic DC subsets nor the development and phenotype of BMDC.
T6767 19537-19659 Sentence denotes However, loss of SHARPIN significantly diminished the capacity of BMDC to secrete inflammatory cytokines and nitric oxide.
T6768 19660-19830 Sentence denotes The attenuated cytokine production was not due to the presence of anti-inflammatory inhibitors, and can be largely explained by selective inactivation of NF-κB signaling.
T6769 19831-19970 Sentence denotes Stimulated cpdm BMDC exhibited Th2-biased T cell-polarizing capabilities, consistent with the Th2 cytokine-dominant phenotype in cpdm mice.
T6770 19971-20152 Sentence denotes Together, these results indicate an indispensible role of SHARPIN in regulating DC immunological functions, disruption of which may contribute to the development of immune diseases.
T6771 20153-20290 Sentence denotes Since WT and cpdm mice are both specific pathogen-free, the nature of the trigger of the severe inflammation in cpdm mice is not obvious.
T6772 20291-20484 Sentence denotes One such initiating factor could be endogenous apoptotic and/or necrotic cells that can release danger-associated molecular patterns (DAMPs) to launch and amplify an inflammatory response [32].
T6773 20485-20644 Sentence denotes Such ‘sterile’ inflammation could be initiated and take place in all organs and tissues affected in cpdm mice, thus causing multi-organ inflammatory disorders.
T6774 20645-20700 Sentence denotes This hypothesis is supported by several recent studies.
T6775 20701-20997 Sentence denotes Fibroblasts of cpdm mice are highly sensitive to TNFα-induced cell death and the cpdm phenotype can be partially rescued by deletion of TNF [7], [8], suggesting that deficiency of SHARPIN compromises the anti-apoptotic mechanisms in cpdm mice resulting in cell death-induced inflammatory disease.
T6776 20998-21156 Sentence denotes Apoptosis of keratinocytes is a prominent feature of the skin lesions in cpdm mice [33] and this is mediated by caspase-dependent mitochondrial pathways [34].
T6777 21157-21397 Sentence denotes These induced and/or intrinsic apoptotic cells can release various types of DAMPs that exert their pro-inflammatory properties by activating DC through pattern recognition receptors [32], [35], such as HMGB1 recognized by TLR2/4 [36], [37].
T6778 21398-21580 Sentence denotes Consistent with the impaired Th1 immune response in cpdm mice [5], stimulated BMDC weakly polarized Th1 differentiation, but strongly supported the development of Th2 effector cells.
T6779 21581-21786 Sentence denotes Combined with the dramatic effect of IL12 treatment on the phenotype of these mice [5], this suggests that the Th2-biased systemic inflammation in cpdm mice is caused by reduced IL12P70 production from DC.
T6780 21787-21996 Sentence denotes The importance of IL12P70 in regulating Th1 and Th2 immune responses in mice was clearly demonstrated in IL12P35- and IL12P40-deficient mice [38], [39] and through clinical studies in human patients [40]–[42].
T6781 21997-22160 Sentence denotes NF-κB, TBK/IRF3, and MAPK signaling are important pathways activated by LPS or poly I:C and disruption of either pathway may lead to decreased cytokine expression.
T6782 22161-22429 Sentence denotes NF-κB activation was selectively inhibited in LPS and poly I:C stimulated cpdm BMDC compared to wild type, while activation of TBK1/IRF3, ERK1/2, or p38 was not, indicating that disrupted NF-κB signaling in cpdm DC is responsible for the defective cytokine expression.
T6783 22430-22566 Sentence denotes These results seem to contradict a recent study that found increased levels of NF-κB activation and IL1 transcription in cpdm mice [43].
T6784 22567-22738 Sentence denotes This difference can probably be attributed to the different cells and tissues used in these studies, and may point to cell- and tissue- type specific functions of SHARPIN.
T6785 22739-22829 Sentence denotes This is consistent with recent reports of tissue-specific effects of NF-κB signaling [44].
T6786 22830-23063 Sentence denotes Ubiquitous activation of NF-κB by removing inhibitors such as A20 and ITCH through genetic manipulation results in widespread inflammation, consistent with the role of NF-κB in the production of pro-inflammatory mediators [45], [46].
T6787 23064-23248 Sentence denotes However, selective inhibition of NF-κB activation in parenchymal cells of the skin, liver, and intestine results in chronic inflammation driven by NF-κB competent leukocytes [47]–[50].
T6788 23249-23441 Sentence denotes This indicates that a balance between the pro-inflammatory role of NF-κB in leukocytes and the anti-inflammatory role in parenchymal cells is critical in the maintenance of tissue homeostasis.
T6789 23442-23596 Sentence denotes Experiments with mice with cell- and tissue-specific deletion of Sharpin, currently under way, will help to elucidate the role of SHARPIN in inflammation.
T6790 23597-23778 Sentence denotes Despite defective NF-κB activation in the absence of Sharpin expression, there was no significant change in splenic DC populations or expression of co-stimulatory molecules on BMDC.
T6791 23779-23982 Sentence denotes Different NF-κB subunits involved in the canonical and non-canonical branches of the NF-κB signaling pathway have distinct functions to control specific aspects of DC development and function [51], [52].
T6792 23983-24227 Sentence denotes The non-canonical pathway (p100 processing to produce p52) appears to be intact in SHARPIN-deficient cells [7], [9] suggesting that the NF-κB heterodimer p52/RELB is sufficient to maintain the normal regulation of DC homeostasis and maturation.
T6793 24228-24343 Sentence denotes The molecular basis by which the Sharpin mutation causes reduced NF-κB activation in BMDC remains to be determined.
T6794 24344-24449 Sentence denotes LPS and poly I:C used here are well-defined ligands that specifically engage TLR4 and TLR3, respectively.
T6795 24450-24641 Sentence denotes The expression profile of surface TLR4 complexes is similar between WT and cpdm BMDC suggesting that defective NF-κB activation is not a result of differential TLR expression on target cells.
T6796 24642-24796 Sentence denotes LPS engages TLR4 to activate MYD88-dependent and TRIF-dependent pathways, whereas TLR3 stimulated by poly I:C only triggers TRIF-dependent signaling [53].
T6797 24797-24990 Sentence denotes The defective NF-κB activation by both stimuli suggests that the Sharpin mutation interferes with the protein adaptors or kinases shared by both signaling pathways, such as RIP1 and TRAF6 [18].
T6798 24991-25229 Sentence denotes Recent studies demonstrated that SHARPIN interacts with HOIP to form LUBAC that exerts its linear-ubiquitin-chain-ligase activity on NF-κB signaling players RIP1 and NEMO [7], an essential step for intact TNFα-stimulated NF-κB activation.
T6799 25230-25347 Sentence denotes The Sharpin null mutation disrupts the ubiquitylation process and abrogates the TNFα-induced NF-κB signaling pathway.
T6800 25348-25532 Sentence denotes Since TNFR and TLR partially share their downstream signaling cascades, a similar ubiquitin-mediated regulation may hold true for SHARPIN in LPS- and poly I:C-induced NF-κB activation.
T6801 25533-25687 Sentence denotes In summary, the present study identified an indispensible role of SHARPIN in the production of pro-inflammatory mediators and TLR-induced NF-κB signaling.
T6802 25688-25818 Sentence denotes The impaired Th1-stimulating ability of Sharpin-deficient DC may account for the Th2-dominant inflammatory phenotype of cpdm mice.
T6803 25819-25902 Sentence denotes The balance between Th1 and Th2 differentiation is critical for immune homeostasis.
T6804 25903-26127 Sentence denotes A better understanding of how such balance is maintained will help design cytokine treatment for human diseases with Th2-biased cytokine secretion similar to the mouse cpdm, such as allergies and hypereosinophilic syndromes.
T9577 26152-26168 Sentence denotes Ethics Statement
T9578 26169-26297 Sentence denotes All mouse work was carried out in strict accordance with protocols approved by the Institutional Animal Care and Use Committees.
T9629 26299-26303 Sentence denotes Mice
T9630 26304-26497 Sentence denotes Specific-pathogen free colonies of C57BL/KaLawRij-Sharpincpdm/RijSunJ (JR#7599) and WT mice were obtained from The Jackson Laboratory (Bar Harbor, ME) and were maintained in a barrier facility.
T9631 26498-26645 Sentence denotes Normal littermate controls were either +/+ or +/Sharpincpdm. These control animals were phenotypically indistinguishable and are referred to as WT.
T9632 26646-26708 Sentence denotes Sex-matched WT and mutant mice were used at 6–10 weeks of age.
T9633 26709-26890 Sentence denotes For some experiments, cpdm mice were crossed with transgenic mice with a bacterial artificial chromosome (BAC) containing the Sharpin gene (FVB/NJ-Tg(RP24-173I23)1Sun/Sun, JR#8279).
T9634 26891-27029 Sentence denotes These mice were backcrossed onto the C57BL/KaLawRij-Sharpincpdm/RijSunJ background and N4 mice were used in the experiments reported here.
T9635 27030-27161 Sentence denotes All mouse work was carried out in strict accordance with the approved protocols by the Institutional Animal Care and Use Committee.
T9960 27163-27190 Sentence denotes Constructs and transfection
T9961 27191-27298 Sentence denotes The complementary DNA (cDNA) of the mouse Sharpin gene was cloned and amplified from RAW264.7 RNA extracts.
T9962 27299-27492 Sentence denotes The primer sequences were forward, 5′-CC ATG GCG ATG TCG CCG CCC GCC GGC GGT; reverse, 5′- AAG CTT CTA GGT GGA AGC TGC AGC AAG A. The Sharpin cDNA was cloned into expression vector pFLAG-CMV-2.
T9963 27493-27582 Sentence denotes Murine fibroblasts and macrophages were were used to express recombinant SHARPIN protein.
T9964 27583-27663 Sentence denotes Cells (2×104) were seeded in 96-well treated plates the day before transfection.
T9965 27664-27770 Sentence denotes After overnight incubation, 200 ng pFLAG-SHARPIN plasmids were transfected with 0.5 ul Lipofectamine 2000.
T9966 27771-27834 Sentence denotes 24 hours later, cells were incubated with fresh culture medium.
T9967 27835-27945 Sentence denotes After another 24 hours, cells were lysed to confirm the FLAG-SHARPIN expression by immunoblots with anti-FLAG.
T9968 27946-28081 Sentence denotes Cells with no transfection and transfected with empty vector pFLAG-CMV-2 were used as negative control in all transfection experiments.
T10335 28083-28101 Sentence denotes Generation of BMDC
T10336 28102-28156 Sentence denotes BMDC were developed as previously reported [54], [55].
T10337 28157-28206 Sentence denotes Cells were collected after 10–12 days of culture.
T10338 28207-28263 Sentence denotes The cell yield was 2–3×107 cells/mouse with 80–95% BMDC.
T10423 28265-28305 Sentence denotes Phenotype of BMDC and splenic DC subsets
T10424 28306-28409 Sentence denotes The phenotype of BMDC was determined before and after 24 hour culture in the presence of 100 ng/mL LPS.
T10425 28410-28816 Sentence denotes The cells were incubated in PBS with 0.1% NaN3, 1% BSA and 10% normal rabbit serum for 20 minutes on ice, washed and incubated for 30 minutes on ice with Alex Fluor-labeled anti-CD11c (MCD11C20, CALTAG) in combination with PE- anti-CD40 (3/23, BD Biosciences), PE-anti-CD80 (16-10A1, eBioscience), PE-anti-CD86 (P03.1, eBioscience), PE-anti-CD14 (Sa2.8, eBioscience) and biotinylated anti-TLR4 (BioLegend).
T10426 28817-28939 Sentence denotes The cells incubated with biotinylated anti-TLR4/MD-2 were washed twice and incubated for 30 minutes on ice with avidin-PE.
T10427 28940-29044 Sentence denotes To isolate splenic DC, spleens were collagenase digested and subject to Percoll gradient centrifugation.
T10428 29045-29216 Sentence denotes The bands at the 35–55% interface were collected to stain with PE-anti-CD11c (HL3, BioLegend), APC-anti-PDCA-1 (927, BioLegend) and FITC-anti-CD8α (53–6.7, BD BioScience).
T10429 29217-29309 Sentence denotes The cells were washed twice, fixed in 2% paraformaldehyde, and stored at 4°C until analysis.
T10430 29310-29372 Sentence denotes Flow cytometry was performed in an Excel (Coulter) instrument.
T10431 29373-29509 Sentence denotes Dead cells were omitted from the analysis by gating on forward and 90° light scatter, and 10,000 cells were analyzed by FlowJo software.
T10960 29511-29543 Sentence denotes BMDC-T cell in vitro interaction
T10961 29544-29640 Sentence denotes WT and cpdm BMDC (5×104) were stimulated with medium, 1 µg/mL LPS, 25 µg/mL and 5 µg/mL Pam3CYS.
T10962 29641-29698 Sentence denotes 24 hours later, cells were collected and washed with PBS.
T10963 29699-29877 Sentence denotes Allogeneic naïve CD4+ T cells were isolated from spleens of BALB/c mice by negative selection kit (Invitrogen) and were then added at 2.5×105 and co-cultured with activated BMDC.
T10964 29878-29983 Sentence denotes After 5 days, supernatant was collected and the secretion of IFNγ, IL4, IL2, and IL17A measured by ELISA.
T10965 29984-30141 Sentence denotes Negative controls are 1) stimulated BMDC without co-culture with allogeneic CD4+ T cells; 2) allogeneic CD4+ T cells without co-culture with stimulated BMDC.
T10966 30142-30212 Sentence denotes Both negative controls show no production of aforementioned cytokines.
T11351 30214-30236 Sentence denotes RNA expression by BMDC
T11352 30237-30484 Sentence denotes BMDC were cultured at 106 cells/mL in 10 mL of RPMI-1640 complete medium in the presence or absence of 100 ng/mL LPS or 25 µg/mL poly I:C. After 1 and 2 hours, RNA was isolated with TRI-reagent (Sigma) according to the manufacturer's instructions.
T11353 30485-30567 Sentence denotes The expression of Il6, Il12p40, Gmcsf, and Sharpin mRNA was determined by qRT-PCR.
T11354 30568-30970 Sentence denotes Primers and probes were purchased from Applied Biosystems. Reverse transcription was performed at 42°C for 60 minutes with the final denaturation step at 90°C for 5 minutes in 30 µl containing 0.5 µg of total RNA. dNTPs, oligo(dT)15 primer, recombinant RNasin Ribonuclease Inhibitor, and M-MLV Reverse Transcriptase (all from Promega, Madison, WI) were used according to the manufacturer's instruction.
T11355 30971-31304 Sentence denotes Reverse transcription was done in a PTC-200 Peltier Thermal Cycler (MJ Research, Watertown, MA). qRT-PCR was performed in ABI Prism 7700 Sequence Detection System with TaqMan® Gene Expression Assays (Applied Biosystems, Foster City, CA) for mouse Actb, IL6, IL12p40, Ifnβ, Gmcsf, and Sharpin according to the manufacturer's protocol.
T11356 31305-31378 Sentence denotes The endogenous standard for normalization of the target gene was β-actin.
T11357 31379-31448 Sentence denotes Relative gene expression was calculated using the 2−ΔΔCt method [56].
T11921 31450-31485 Sentence denotes In vitro cytokine secretion by BMDC
T11922 31486-31625 Sentence denotes The BMDC were cultured in triplicate wells of 24-well or 96-well plates at 106 or 105 cells/mL, respectively, in RPMI-1640 complete medium.
T11923 31626-31767 Sentence denotes The cells were washed and stimulated with 100 ng/mL LPS or 25 µg/mL poly I:C. After 24 hours, supernatants were harvested for ELISA analysis.
T11924 31768-31852 Sentence denotes The presence of nitrite in the supernatants was determined using the Griess reagent.
T12083 31854-31872 Sentence denotes Immunofluorescence
T12084 31873-31941 Sentence denotes Fibroblast and macrophage cells were transfected with pFLAG-SHARPIN.
T12085 31942-32085 Sentence denotes 48 hours later, cells were washed briefly with PBS, then fixed in cold methanol at −20°C for 10 minutes and cold acetone at -20°C for 1 minute.
T12086 32086-32162 Sentence denotes Incubate cells with PBS+1% BSA for 15 minutes to block non-specific binding.
T12087 32163-32308 Sentence denotes Fixed cells were then incubated with anti-FLAG (1∶100) as the primary antibody at room temperature for 1 hour, followed by three 5-minute washes.
T12088 32309-32473 Sentence denotes Cells were further incubated with goat anti-rabbit IgG-FITC (1∶100) as the secondary antibody at room temperature for 30 minutes, followed by three 5-minute washes.
T12089 32474-32537 Sentence denotes Cells were then examined using inverse fluorescence microscope.
T12382 32539-32550 Sentence denotes Immunoblots
T12383 32551-32694 Sentence denotes After adding stimulating ligands, 100 ng/mL LPS or 25 µg/mL poly I:C, BMDC were collected and lysed at 0-, 15-, 30-, and 60-minute time points.
T12384 32695-32901 Sentence denotes Immunoblots were performed with antibodies (Cell Signaling Technology) against p-IKK1/2(#2697), p-IκBα (#9246), IκBα (#4814), p-TBK1(#5483), p-p38 (#9216), p38 (#9212), p-ERK1/2 (#4376), and ERK1/2 (#4695).
T12385 32902-32978 Sentence denotes Beta-actin (sc-47778, Santa Cruz Biotechnology) was used as loading control.
T12679 32980-33000 Sentence denotes Statistical analysis
T12680 33001-33033 Sentence denotes Data are expressed as mean ± SD.
T12681 33034-33150 Sentence denotes The statistical significance of differences of means between experimental groups was determined by Students' t-test.
T12763 6096-6137 Sentence denotes In vivo and in vitro features of SHARPIN.
T12764 6138-6451 Sentence denotes (A) COILS and MotifScan programs were used to predict the presence of CC (coiled-coil) domain, UBL (ubiquitin-like) domain and ZFRBP (zinc-finger Ran-Binding protein 2) domain which form similar motif patterns in the SHARPIN protein of human, mouse and rat origins. (B) Eight week-old females of WT and cpdm mice.
T12765 6452-6711 Sentence denotes The mutant mice (above) develop progressive skin inflammation starting at about four weeks. (C) Extramedullary hematopoiesis causes marked enlargement of the spleen of cpdm mice. (D) BMDC were incubated in the absence or presence of 100 ng/ml LPS for 4 hours.
T12766 6712-6834 Sentence denotes The mRNA level of Sharpin was measured by qRT-PCR and presented relative to the mRNA expression in non-stimulated WT BMDC.
T12767 6835-7015 Sentence denotes Bars represent the mean ± s.d. of 3 mice. * P<0.05; ** P<0.001. (E) Fibroblast (NIH3T3) and macrophages (RAW264.7) cells were transfected with the expression plasmid pFLAG-SHARPIN.
T12768 7016-7114 Sentence denotes After 48 hours, cells were fixed and probed with anti-FLAG and FITC-conjugated secondary antibody.
T12769 7115-7145 Sentence denotes Nuclei were stained with DAPI.
T12770 7146-7227 Sentence denotes In both transfected cell lines, FLAG-SHARPIN was found to be cytoplasm-localized.
T13265 8298-8361 Sentence denotes Effect of Sharpin mutation on DC subpopulations and maturation.
T13266 8362-8457 Sentence denotes (A) Spleens from WT and cpdm mice were isolated and subject to collagenase and DNase digestion.
T13267 8458-8569 Sentence denotes The obtained splenic homogenates were centrifuged over a Percoll gradient (35% and 55% density) for 15 minutes.
T13268 8570-8917 Sentence denotes The bands at the 35%-medium and the 35–55% interface were pooled, washed and stained with a combination of various antibodies to stain different DC subsets, conventional CD11c+CD8α+, CD11c+CD8α− and plasmacytoid DC (CD11c−PDCA-1+).The top panels were gated on FSChiSSClo cells to show separate populations of CD11c+PDCA-1− and CD11c−PDCA-1+ cells.
T13269 8918-9058 Sentence denotes Further gating on the CD11c+PDCA-1− subpopulation gave the bottom panel that showed two distinct pools of CD11c+CD8α+ and CD11c+CD8α− cells.
T13270 9059-9287 Sentence denotes Percentages were calculated based on the parental population and were additionally shown as bar graphs (n = 2) (B). (C) WT and cpdm BMDC (5×105) cells were stimulated with medium, 100 ng/ml LPS or 25 µg/ml poly I:C for 24 hours.
T13271 9288-9405 Sentence denotes The cells were labeled with PE-labeled anti-CD40, anti-CD80, and anti-CD86, and subjected to flow cytometry analysis.
T13272 9406-9469 Sentence denotes The populations shown in histograms were gated on CD11c+ cells.
T13273 9470-9514 Sentence denotes Unstained cells served as negative controls.
T13274 9515-9573 Sentence denotes Results are representative of two independent experiments.
T13806 11390-11467 Sentence denotes Defective production of pro-inflammatory mediators from stimulated cpdm BMDC.
T13807 11468-11628 Sentence denotes (A–D) Cultured WT and cpdm BMDC (1×105 cells in 0.1 ml complete medium) were washed and stimulated with medium, 100 ng/ml LPS or 25 µg/ml poly I:C for 24 hours.
T13808 11629-11868 Sentence denotes Supernatants were collected for ELISA of IL12P70, IL6, and GMCSF, and for quantification of nitric oxide (NO). (E–F) Gradient numbers (1×104, 2×104, and 4×104 cells in 0.1 mL complete medium) of BMDC were used for100 ng/mL LPS stimulation.
T13809 11869-12061 Sentence denotes After 24 hours, the amounts of IL12P70 and IL6 from the supernatant were measured. (G) The cpdm mice rescued by Sharpin-containing BAC had complete remission of the inflammatory phenotype [2].
T13810 12062-12179 Sentence denotes BMDC developed from cpdm and rescued cpdm mice were plated (1×106 cells in 0.3 mL) and stimulated with 100 ng/mL LPS.
T13811 12180-12259 Sentence denotes After 24 hours, supernatants were collected for analysis of IL12P70 production.
T13812 12260-12339 Sentence denotes Data are representative of three independent experiments. * P<0.01; ** P<0.005.
T14256 12384-12447 Sentence denotes Decreased mRNA levels of inflammatory cytokines from cpdm BMDC.
T14257 12448-12601 Sentence denotes Cultured WT and cpdm BMDC (5×105 cells in 0.2 ml complete medium) were washed and stimulated with 100 ng/ml LPS (A,C,E,G) or 25 µg/ml poly I:C (B,D,F,H).
T14258 12602-12758 Sentence denotes At 0, 1 and 2 hours, total RNA was extracted and subject to qRT-PCR to measure the expression of Il12p40 (A,B), Il6 (C,D), Gmcsf (E,F), and Ifnb (G,H) mRNA.
T14259 12759-12784 Sentence denotes Bars represent mean ± SD.
T14260 12785-12840 Sentence denotes Data are representative of two independent experiments.
T14580 14284-14376 Sentence denotes Normal TLR4 and MD2 expression and decreased IL10 secretion and A20 expression by cpdm BMDC.
T14581 14377-14519 Sentence denotes (A) Unstimulated WT and cpdm BMDC (5×105) were labeled with PE-labeled anti-TLR4/MD2 or anti-CD14 and then subject to flow cytometry analysis.
T14582 14520-14722 Sentence denotes Unstained cells serve as negative controls. (B) Cultured WT and cpdm BMDC (1×105 cells in 0.1 ml complete medium) were washed and stimulated with medium, 100 ng/ml LPS or 25 µg/ml poly I:C for 24 hours.
T14583 14723-15010 Sentence denotes Supernatants were collected for ELISA of IL10. (C) Cultured WT and cpdm BMDC (5×105 cells in 0.2 ml complete medium) were washed and stimulated with 100 ng/ml LPS or 25 µg/ml poly I:C. At 0, 1, and 2 hours, total RNA was extracted and subject to qRT-PCR to measure the production of A20.
T14993 16085-16128 Sentence denotes Inhibition of NF-κB signaling in cpdm BMDC.
T14994 16129-16250 Sentence denotes WT and cpdm BMDC (2×106 cells in 0.5 mL complete medium) were stimulated with 100 ng/mL LPS (A) or 25 µg/mL poly I:C (B).
T14995 16251-16439 Sentence denotes At 0, 15, 30, and 60 minutes, whole-cell lysates were obtained and subject to immunoblots with antibodies against proteins involved in NF-κB, TBK1/IRF3, ERK1/2, and p38 signaling pathways.
T14996 16440-16623 Sentence denotes Beta-actin was used as loading control. (C) Cellular levels of p-IKK1/2 and p-IκBα in LPS- or poly I:C-stimulated BMDC were quantitated with ImageJ (NIH) and presented as trend lines.
T14997 16624-16691 Sentence denotes Results are representative of at least two independent experiments.
T15334 18492-18576 Sentence denotes Stimulated cpdm BMDC induced Th2-biased cytokine production from naïve CD4+ T cells.
T15335 18577-18735 Sentence denotes WT and cpdm BMDC (5×104 cells in 0.1 mL complete medium; MHC haplotype: H-2b) were incubated with medium, 100 ng/mL LPS, 25 µg/mL poly I:C or 5 µg/mL Pam3CYS.
T15336 18736-18908 Sentence denotes After 24 hours, cells were washed with PBS and incubated with freshly isolated allogeneic naïve CD4+ T cells (2.5×105 cells in 0.1 mL complete medium; MHC haplotype: H-2d).
T15337 18909-19014 Sentence denotes After 5 days, supernatants were collected and the secretion of IFNγ, IL4, and IL2 were measured by ELISA.
T15338 19015-19172 Sentence denotes Negative controls are 1) stimulated BMDC without co-culture with allogeneic CD4+ T cells; 2) allogeneic CD4+ T cells without co-culture with stimulated BMDC.
T15339 19173-19282 Sentence denotes Samples from both negative controls had no detectable production of the aforementioned cytokines (not shown).
T15340 19283-19342 Sentence denotes Results are analyzed based on 2–4 mice per group. * P<0.05.
T2217 4735-4762 Sentence denotes Characterization of Sharpin
T2218 4764-4854 Sentence denotes Orthologs of SHARPIN protein are found in various species, including human, mouse and rat.
T2219 4855-5066 Sentence denotes Motif prediction programming, using COILS [11] and MotifScan [12], suggests that SHARPIN contains a coiled-coil (CC) domain, a ubiquitin-like (UBL) domain, and a zinc-finger Ran-binding protein 2 (ZFRBP) domain.
T2220 5067-5271 Sentence denotes These functional motifs constitute similar domain profiles that are present in the SHARPIN protein of all three origins (Fig. 1A), suggesting that SHARPIN exerts highly conserved functions across species.
T2221 5272-5508 Sentence denotes Spontaneous mutations in the mouse Sharpin gene results in a complex inflammatory phenotype characterized by severe dermatitis (Fig. 1B), systemic inflammation and an enlarged spleen (Fig. 1C) caused by extramedullary hematopoiesis [3].
T2222 5509-5677 Sentence denotes The endogenous expression of Sharpin mRNA in BMDC was determined by quantitative real time-PCR (qRT-PCR) following culture in medium only or after stimulation with LPS.
T2223 5678-5800 Sentence denotes Sharpin mRNA was present in BMDC generated from WT mice (Fig. 1D) and its level was modestly decreased by LPS stimulation.
T2224 5801-5895 Sentence denotes There was a significant reduction of Sharpin mRNA (6–7-fold) in BMDC generated from cpdm mice.
T2225 5896-6051 Sentence denotes Transfection of Flag-tagged Sharpin in fibroblasts (NIH3T3) and macrophages (RAW264.7) indicated cytoplasmic localization of the SHARPIN protein (Fig. 1E).
T2872 7229-7282 Sentence denotes Phenotyping splenic DC and BMDC from WT and cpdm mice
T2873 7283-7380 Sentence denotes DC are heterogeneous and can be categorized into multiple subtypes based on surface markers [13].
T2874 7381-7616 Sentence denotes To determine if the Sharpin mutation affects DC development in lymphoid tissues, mouse spleens were examined for the distribution of conventional DC (cDC; CD11c+CD8α+ and CD11c+CD8α−) [13] and plasmacytoid DC (pDC; CD11c−PDCA-1+) [14].
T2875 7617-7729 Sentence denotes The percentages for splenic cDC and pDC were both reduced in cpdm mice when compared with WT controls (Fig. 2A).
T2876 7730-7856 Sentence denotes However, when gated on CD11c+ cDC, the percentages of CD8α+ and CD8α− cells were not affected by SHARPIN deficiency (Fig. 2A).
T1 0-153 Sentence denotes SHARPIN Is Essential for Cytokine Production, NF-κB Signaling, and Induction of Th1 Differentiation by Dendritic Cells Role of SHARPIN in Dendritic Cells
T2 156-164 Sentence denotes Abstract
T3 165-265 Sentence denotes Spontaneous mutations of the Sharpin (SHANK-associated RH domain-interacting protein, other aliases:
T4 266-448 Sentence denotes Rbckl1, Sipl1) gene in mice result in systemic inflammation that is characterized by chronic proliferative dermatitis and dysregulated secretion of T helper1 (Th1) and Th2 cytokines.
T5 449-541 Sentence denotes The cellular and molecular mechanisms underlying this inflammatory phenotype remain elusive.
T6 542-712 Sentence denotes Dendritic cells may contribute to the initiation and progression of the phenotype of SHARPIN-deficient mice because of their pivotal role in innate and adaptive immunity.
T7 713-839 Sentence denotes Here we show by flow cytometry that SHARPIN- deficiency did not alter the distribution of different DC subtypes in the spleen.
T8 840-1074 Sentence denotes In response to TOLL-like receptor (TLR) agonists LPS and poly I:C, cultured bone marrow-derived dendritic cells (BMDC) from WT and mutant mice exhibited similar increases in expression of co-stimulatory molecules CD40, CD80, and CD86.
T9 1075-1226 Sentence denotes However, stimulated SHARPIN-deficient BMDC had reduced transcription and secretion of pro-inflammatory mediators IL6, IL12P70, GMCSF, and nitric oxide.
T10 1227-1399 Sentence denotes Mutant BMDC had defective activation of NF-κB signaling, whereas the MAPK1/3 (ERK1/2) and MAPK11/12/13/14 (p38 MAP kinase isoforms) and TBK1 signaling pathways were intact.
T11 1400-1576 Sentence denotes A mixed lymphocyte reaction showed that mutant BMDC only induced a weak Th1 immune response but stimulated increased Th2 cytokine production from allogeneic naïve CD4+ T cells.
T12 1577-1756 Sentence denotes In conclusion, loss of Sharpin in mice significantly affects the immune function of DC and this may partially account for the systemic inflammation and Th2-biased immune response.
T13 1758-1770 Sentence denotes Introduction
T14 1771-1946 Sentence denotes SHARPIN was originally identified in post-synaptic densities of excitatory synapses in the brain of rats [1], but this protein is widely expressed in a variety of tissues [2].
T15 1947-2253 Sentence denotes Two allelic, autosomal recessive mutations in the Sharpin gene occurred spontaneously in two inbred strains of mice, C57B/KaLawRij-Sharpincpdm/Sharpincpdm and CBy.Ocb3/Dem-Sharpincpdm-Dem/Sharpincpdm-Dem, resulting in premature termination of mRNA synthesis and absence of a functional protein product [2].
T16 2254-2449 Sentence denotes Despite different genetic backgrounds, both mutations cause similar inflammatory disease with severe chronic progressive dermatitis and defective development of secondary lymphoid organs [2]–[4].
T17 2450-2520 Sentence denotes The dermatitis becomes clinically apparent at about four weeks of age.
T18 2521-2796 Sentence denotes There are accumulations of eosinophils, neutrophils and macrophages in the skin of Sharpincpdm/Sharpincpdm mutant mice (hereafter referred to as cpdm mice) associated with increased expression of Th2 cytokines in the skin and in the supernatants of activated splenocytes [5].
T19 2797-2984 Sentence denotes The mice have an impaired delayed type hypersensitivity response and decreased secretion of IFNγ [5], indicating a defect in Th1 immune responses and a bias towards a Th2 immune response.
T20 2985-3087 Sentence denotes Systemic treatment of cpdm mice with recombinant IL12 caused complete remission of the dermatitis [5].
T21 3088-3294 Sentence denotes Neutralization of IL5 by antibody treatment or crosses with IL5-deficient mice reduced the number of circulating and cutaneous eosinophils, but failed to reduce the onset and severity of the dermatitis [6].
T22 3295-3492 Sentence denotes Recently, three independent groups identified SHARPIN as an essential component of the linear ubiquitin chain assembly complex (LUBAC) that regulates TNFα-induced canonical NF-κB signaling [7]–[9].
T23 3493-3667 Sentence denotes SHARPIN-deficient mouse embryonic fibroblast (MEF) were sensitized to TNFα-induced apoptosis and cell death was implicated as a factor in the dermatitis of cpdm mice [7]–[9].
T24 3668-3820 Sentence denotes Dendritic cells (DC) have a sentinel role in sensing pathogen or danger signals and initiate and direct activation of the adaptive immune response [10].
T25 3821-3970 Sentence denotes Activated and mature DC can carry processed antigenic peptides, migrate to lymphoid organs, and induce T-cell-mediated immune responses or tolerance.
T26 3971-4102 Sentence denotes DC direct the differentiation of CD4+ T cells, and hence the type of immune response, through the selective secretion of cytokines.
T27 4103-4238 Sentence denotes We hypothesized that defective cytokine secretion by DC contributed to the Th2-biased inflammatory phenotype in SHARPIN-deficient mice.
T28 4239-4420 Sentence denotes The studies reported here found that lack of SHARPIN protein in BMDC caused defective expression of pro-inflammatory mediators and impaired NF-κB activation upon ligand stimulation.
T29 4421-4526 Sentence denotes The ability of cpdm BMDC to stimulate Th1 cytokine production in allogeneic CD4+ T cells was compromised.
T30 4527-4724 Sentence denotes Taken together, these results reveal that SHARPIN is a novel regulatory molecule in DC biology and suggest that the dysregulated function of SHARPIN-deficient DC plays a role in the cpdm phenotype.
T31 4726-4733 Sentence denotes Results
T32 4735-4762 Sentence denotes Characterization of Sharpin
T33 4764-4854 Sentence denotes Orthologs of SHARPIN protein are found in various species, including human, mouse and rat.
T34 4855-5066 Sentence denotes Motif prediction programming, using COILS [11] and MotifScan [12], suggests that SHARPIN contains a coiled-coil (CC) domain, a ubiquitin-like (UBL) domain, and a zinc-finger Ran-binding protein 2 (ZFRBP) domain.
T35 5067-5271 Sentence denotes These functional motifs constitute similar domain profiles that are present in the SHARPIN protein of all three origins (Fig. 1A), suggesting that SHARPIN exerts highly conserved functions across species.
T36 5272-5508 Sentence denotes Spontaneous mutations in the mouse Sharpin gene results in a complex inflammatory phenotype characterized by severe dermatitis (Fig. 1B), systemic inflammation and an enlarged spleen (Fig. 1C) caused by extramedullary hematopoiesis [3].
T37 5509-5677 Sentence denotes The endogenous expression of Sharpin mRNA in BMDC was determined by quantitative real time-PCR (qRT-PCR) following culture in medium only or after stimulation with LPS.
T38 5678-5800 Sentence denotes Sharpin mRNA was present in BMDC generated from WT mice (Fig. 1D) and its level was modestly decreased by LPS stimulation.
T39 5801-5895 Sentence denotes There was a significant reduction of Sharpin mRNA (6–7-fold) in BMDC generated from cpdm mice.
T40 5896-6051 Sentence denotes Transfection of Flag-tagged Sharpin in fibroblasts (NIH3T3) and macrophages (RAW264.7) indicated cytoplasmic localization of the SHARPIN protein (Fig. 1E).
T41 6052-6137 Sentence denotes 10.1371/journal.pone.0031809.g001 Figure 1 In vivo and in vitro features of SHARPIN.
T42 6138-6451 Sentence denotes (A) COILS and MotifScan programs were used to predict the presence of CC (coiled-coil) domain, UBL (ubiquitin-like) domain and ZFRBP (zinc-finger Ran-Binding protein 2) domain which form similar motif patterns in the SHARPIN protein of human, mouse and rat origins. (B) Eight week-old females of WT and cpdm mice.
T43 6452-6711 Sentence denotes The mutant mice (above) develop progressive skin inflammation starting at about four weeks. (C) Extramedullary hematopoiesis causes marked enlargement of the spleen of cpdm mice. (D) BMDC were incubated in the absence or presence of 100 ng/ml LPS for 4 hours.
T44 6712-6834 Sentence denotes The mRNA level of Sharpin was measured by qRT-PCR and presented relative to the mRNA expression in non-stimulated WT BMDC.
T45 6835-7015 Sentence denotes Bars represent the mean ± s.d. of 3 mice. * P<0.05; ** P<0.001. (E) Fibroblast (NIH3T3) and macrophages (RAW264.7) cells were transfected with the expression plasmid pFLAG-SHARPIN.
T46 7016-7114 Sentence denotes After 48 hours, cells were fixed and probed with anti-FLAG and FITC-conjugated secondary antibody.
T47 7115-7145 Sentence denotes Nuclei were stained with DAPI.
T48 7146-7227 Sentence denotes In both transfected cell lines, FLAG-SHARPIN was found to be cytoplasm-localized.
T49 7229-7282 Sentence denotes Phenotyping splenic DC and BMDC from WT and cpdm mice
T50 7283-7380 Sentence denotes DC are heterogeneous and can be categorized into multiple subtypes based on surface markers [13].
T51 7381-7616 Sentence denotes To determine if the Sharpin mutation affects DC development in lymphoid tissues, mouse spleens were examined for the distribution of conventional DC (cDC; CD11c+CD8α+ and CD11c+CD8α−) [13] and plasmacytoid DC (pDC; CD11c−PDCA-1+) [14].
T52 7617-7729 Sentence denotes The percentages for splenic cDC and pDC were both reduced in cpdm mice when compared with WT controls (Fig. 2A).
T53 7730-7856 Sentence denotes However, when gated on CD11c+ cDC, the percentages of CD8α+ and CD8α− cells were not affected by SHARPIN deficiency (Fig. 2A).
T54 7857-8132 Sentence denotes Since the spleen of cpdm mice is markedly enlarged and contains three times as many cells (Fig. 1C), the different percentages of splenic cDC and pDC between WT and mutant mice reflect the increased number of total spleen cells rather than a reduction in cDC and pDC numbers.
T55 8133-8253 Sentence denotes These data indicate that the Sharpin mutation does not affect the distribution of the examined DC subsets in the spleen.
T56 8254-8361 Sentence denotes 10.1371/journal.pone.0031809.g002 Figure 2 Effect of Sharpin mutation on DC subpopulations and maturation.
T57 8362-8457 Sentence denotes (A) Spleens from WT and cpdm mice were isolated and subject to collagenase and DNase digestion.
T58 8458-8569 Sentence denotes The obtained splenic homogenates were centrifuged over a Percoll gradient (35% and 55% density) for 15 minutes.
T59 8570-8917 Sentence denotes The bands at the 35%-medium and the 35–55% interface were pooled, washed and stained with a combination of various antibodies to stain different DC subsets, conventional CD11c+CD8α+, CD11c+CD8α− and plasmacytoid DC (CD11c−PDCA-1+).The top panels were gated on FSChiSSClo cells to show separate populations of CD11c+PDCA-1− and CD11c−PDCA-1+ cells.
T60 8918-9058 Sentence denotes Further gating on the CD11c+PDCA-1− subpopulation gave the bottom panel that showed two distinct pools of CD11c+CD8α+ and CD11c+CD8α− cells.
T61 9059-9287 Sentence denotes Percentages were calculated based on the parental population and were additionally shown as bar graphs (n = 2) (B). (C) WT and cpdm BMDC (5×105) cells were stimulated with medium, 100 ng/ml LPS or 25 µg/ml poly I:C for 24 hours.
T62 9288-9405 Sentence denotes The cells were labeled with PE-labeled anti-CD40, anti-CD80, and anti-CD86, and subjected to flow cytometry analysis.
T63 9406-9469 Sentence denotes The populations shown in histograms were gated on CD11c+ cells.
T64 9470-9514 Sentence denotes Unstained cells served as negative controls.
T65 9515-9573 Sentence denotes Results are representative of two independent experiments.
T66 9576-9697 Sentence denotes BMDC from in vitro cultures functionally resemble non-lymphoid tissue DC and monocyte-derived inflammatory DC [15], [16].
T67 9698-9752 Sentence denotes The yields of BMDC from WT and cpdm mice were similar.
T68 9753-9851 Sentence denotes BMDC were CD11c+ and MHC II+ with low expression of co-stimulatory molecules CD40, CD80, and CD86.
T69 9852-10009 Sentence denotes The TLR3 ligand poly I:C and the TLR4 ligand LPS each activate overlapping but different signaling pathways and were used to induce DC maturation [17], [18].
T70 10010-10232 Sentence denotes Incubation with the TLR agonists for 24 hours resulted in increased expression of CD40, CD80, and CD86 on BMDC; however, there was no difference in the expression levels of these markers between WT and cpdm BMDC (Fig. 2B).
T71 10233-10327 Sentence denotes Thus, SHARPIN deficiency did not influence the expression of co-stimulatory molecules by BMDC.
T72 10329-10393 Sentence denotes Production of proinflammatory mediators by cpdm BMDC is impaired
T73 10394-10622 Sentence denotes Incubation with LPS or poly I:C resulted in secretion of IL6, IL12P70, and GMCSF from both WT and cpdm BMDC; however, BMDC from cpdm mice produced significantly less of all three cytokines compared with WT BMDC (Fig. 3A–C, E–F).
T74 10623-10838 Sentence denotes The amount of nitric oxide generated by mutant BMDC was also significantly reduced compared with WT cells (Fig. 3D), indicating severely disrupted production of proinflammatory mediators from Sharpin-deficient BMDC.
T75 10839-11129 Sentence denotes In vivo complementation with a Sharpin gene-containing BAC reversed the phenotype of the mutant mice [2], and BMDC generated from these rescued mice secreted significantly more IL12P70 than BMDC from cpdm mice (Fig. 3G), supporting a necessary role of SHARPIN for the production of IL12P70.
T76 11130-11345 Sentence denotes In addition, the transcript levels of the inflammatory cytokines Il6, Il12p40, Gmcsf, and Ifnb were examined, and these were all significantly reduced in stimulated cpdm BMDC when compared with WT controls (Fig. 4).
T77 11346-11467 Sentence denotes 10.1371/journal.pone.0031809.g003 Figure 3 Defective production of pro-inflammatory mediators from stimulated cpdm BMDC.
T78 11468-11628 Sentence denotes (A–D) Cultured WT and cpdm BMDC (1×105 cells in 0.1 ml complete medium) were washed and stimulated with medium, 100 ng/ml LPS or 25 µg/ml poly I:C for 24 hours.
T79 11629-11868 Sentence denotes Supernatants were collected for ELISA of IL12P70, IL6, and GMCSF, and for quantification of nitric oxide (NO). (E–F) Gradient numbers (1×104, 2×104, and 4×104 cells in 0.1 mL complete medium) of BMDC were used for100 ng/mL LPS stimulation.
T80 11869-12061 Sentence denotes After 24 hours, the amounts of IL12P70 and IL6 from the supernatant were measured. (G) The cpdm mice rescued by Sharpin-containing BAC had complete remission of the inflammatory phenotype [2].
T81 12062-12179 Sentence denotes BMDC developed from cpdm and rescued cpdm mice were plated (1×106 cells in 0.3 mL) and stimulated with 100 ng/mL LPS.
T82 12180-12259 Sentence denotes After 24 hours, supernatants were collected for analysis of IL12P70 production.
T83 12260-12339 Sentence denotes Data are representative of three independent experiments. * P<0.01; ** P<0.005.
T84 12340-12447 Sentence denotes 10.1371/journal.pone.0031809.g004 Figure 4 Decreased mRNA levels of inflammatory cytokines from cpdm BMDC.
T85 12448-12601 Sentence denotes Cultured WT and cpdm BMDC (5×105 cells in 0.2 ml complete medium) were washed and stimulated with 100 ng/ml LPS (A,C,E,G) or 25 µg/ml poly I:C (B,D,F,H).
T86 12602-12758 Sentence denotes At 0, 1 and 2 hours, total RNA was extracted and subject to qRT-PCR to measure the expression of Il12p40 (A,B), Il6 (C,D), Gmcsf (E,F), and Ifnb (G,H) mRNA.
T87 12759-12784 Sentence denotes Bars represent mean ± SD.
T88 12785-12840 Sentence denotes Data are representative of two independent experiments.
T89 12842-12939 Sentence denotes Impaired cytokine production by cpdm BMDC is correlated with selective defects in NF-κB signaling
T90 12940-13284 Sentence denotes There are a number of possible explanations for the defective cytokine secretion in stimulated cpdm BMDC, including 1) reduced surface expression of the LPS receptor complex, 2) increased production of anti-inflammatory mediators, 3) increased expression of negative regulators of TLR pathways, and 4) impaired TLR-induced signaling activation.
T91 13285-13452 Sentence denotes We determined the surface expression of the LPS receptor complex that comprises TLR4, the accessory proteins CD14 and myeloid differentiation factor 2 (MD2/LY96) [19].
T92 13453-13580 Sentence denotes Flow cytometric analysis shows that the expression levels of CD14 and TLR4/MD2 between WT and cpdm BMDC were similar (Fig. 5A).
T93 13581-13714 Sentence denotes We then quantified the secretion of the suppressive cytokines IL10 that can inhibit IL12 secretion in an autocrine manner [20], [21].
T94 13715-13931 Sentence denotes The supernatants from LPS-stimulated cpdm BMDC contained significantly lower levels of IL10 than stimulated WT BMDC (Fig. 5B), suggesting that IL10 was not responsible for decreased secretion of IL12P70 by cpdm BMDC.
T95 13932-14048 Sentence denotes Increased expression of a negative regulator of TLR signaling such as A20 [22] may also suppress cytokine secretion.
T96 14049-14239 Sentence denotes However, the transcript level of A20 was lower in LPS-activated cpdm BMDC than WT controls (Fig. 5C), thereby ruling out overexpression of A20 as a factor in the reduced cytokine production.
T97 14240-14376 Sentence denotes 10.1371/journal.pone.0031809.g005 Figure 5 Normal TLR4 and MD2 expression and decreased IL10 secretion and A20 expression by cpdm BMDC.
T98 14377-14519 Sentence denotes (A) Unstimulated WT and cpdm BMDC (5×105) were labeled with PE-labeled anti-TLR4/MD2 or anti-CD14 and then subject to flow cytometry analysis.
T99 14520-14722 Sentence denotes Unstained cells serve as negative controls. (B) Cultured WT and cpdm BMDC (1×105 cells in 0.1 ml complete medium) were washed and stimulated with medium, 100 ng/ml LPS or 25 µg/ml poly I:C for 24 hours.
T100 14723-14907 Sentence denotes Supernatants were collected for ELISA of IL10. (C) Cultured WT and cpdm BMDC (5×105 cells in 0.2 ml complete medium) were washed and stimulated with 100 ng/ml LPS or 25 µg/ml poly I:C.
T101 14908-15010 Sentence denotes At 0, 1, and 2 hours, total RNA was extracted and subject to qRT-PCR to measure the production of A20.
T102 15014-15184 Sentence denotes The transcription of TLR3/4-induced proinflammatory intermediates is tightly regulated by cellular signaling pathways, in particular NF-κB, TBK1/IRF3, and MAPK [23]–[27].
T103 15185-15342 Sentence denotes We next determined if disrupted NF-κB, TBK1/IRF3, and/or MAPK signaling may underlie the impaired cytokine production from stimulated Sharpin-deficient BMDC.
T104 15343-15593 Sentence denotes Stimulus-induced phosphorylation of the IκB kinase (IKK1/2) is an essential step in NF-κB signaling, allowing phosphorylation and proteasome-mediated degradation of the NF-κB inhibitor IκBα to release the NF-κB transcription factors into the nucleus.
T105 15594-15775 Sentence denotes The amount of phosphorylated IKK1/2 (p-IKK1/2) and IκBα (p-IκBα) following incubation with LPS or poly I:C was severely decreased in cpdm BMDC as compared with WT controls (Fig. 6).
T106 15776-15886 Sentence denotes The cpdm BMDC exhibited similar levels of TBK1, ERK1/2, and p38 phosphorylation to those of WT cells (Fig. 6).
T107 15887-16040 Sentence denotes These results indicate that the absence of functional SHARPIN decreased NF-κB activation but did not affect TBK1/IRF3, ERK1/2, and p38 signaling in BMDC.
T108 16041-16128 Sentence denotes 10.1371/journal.pone.0031809.g006 Figure 6 Inhibition of NF-κB signaling in cpdm BMDC.
T109 16129-16250 Sentence denotes WT and cpdm BMDC (2×106 cells in 0.5 mL complete medium) were stimulated with 100 ng/mL LPS (A) or 25 µg/mL poly I:C (B).
T110 16251-16439 Sentence denotes At 0, 15, 30, and 60 minutes, whole-cell lysates were obtained and subject to immunoblots with antibodies against proteins involved in NF-κB, TBK1/IRF3, ERK1/2, and p38 signaling pathways.
T111 16440-16623 Sentence denotes Beta-actin was used as loading control. (C) Cellular levels of p-IKK1/2 and p-IκBα in LPS- or poly I:C-stimulated BMDC were quantitated with ImageJ (NIH) and presented as trend lines.
T112 16624-16691 Sentence denotes Results are representative of at least two independent experiments.
T113 16693-16742 Sentence denotes Th2-biased immunogenicity of stimulated cpdm BMDC
T114 16743-16956 Sentence denotes The defective IL12 production (Fig. 3A) and Th2-dominant cytokine profile in cpdm mice [5] suggest that the absence of SHARPIN affects the ability of cpdm BMDC to induce T cell differentiation into effector cells.
T115 16957-17257 Sentence denotes Co-culture of allogeneic naïve CD4+ T cells with WT BMDC stimulated with LPS or poly I:C elicited robust IFNγ production, whereas the concentration of IFNγ in cpdm BMDC-T cell cultures was significantly lower after LPS stimulation (Fig. 7A), indicating impaired Th1-polarizing abilities of cpdm BMDC.
T116 17258-17394 Sentence denotes In addition to TLR3/4 agonists, the TLR2 ligand Pam3CYS was used since it has been shown to induce both Th1 and Th2 responses [28]–[30].
T117 17395-17507 Sentence denotes Pam3CYS-matured WT BMDC induced robust IFNγ production at a significantly higher level than cpdm BMDC (Fig. 7A).
T118 17508-17688 Sentence denotes The reduced Th1 differentiation following Pam3CYS stimulation is consistent with the recent report of decreased IL12 production in cpdm macrophages following TLR2 stimulation [31].
T119 17689-17856 Sentence denotes In contrast, more Th2-specific IL4 cytokine was produced in the cpdm BMDC co-cultures than the WT control (Fig. 7B), suggesting Th2-skewed immunogenicity of cpdm BMDC.
T120 17857-18025 Sentence denotes The production of Th17-specific cytokine IL17A following LPS stimulation of dendritic cells was similar between stimulated WT and cpdm BMDC cocultures (data not shown).
T121 18026-18266 Sentence denotes Despite the distinct Th1- and Th2-stimulating abilities of WT and cpdm BMDC, they were equally effective in IL2 production from BMDC-T cell co-cultures except for poly I:C stimulation where WT BMDC induced more IL2 than cpdm BMDC (Fig. 7C).
T122 18267-18447 Sentence denotes The Th2-biased stimulating capability of cpdm BMDC when co-cultured with allogeneic naïve CD4+ T cells is consistent with the Th2-dominant cytokine phenotype observed in cpdm mice.
T123 18448-18576 Sentence denotes 10.1371/journal.pone.0031809.g007 Figure 7 Stimulated cpdm BMDC induced Th2-biased cytokine production from naïve CD4+ T cells.
T124 18577-18735 Sentence denotes WT and cpdm BMDC (5×104 cells in 0.1 mL complete medium; MHC haplotype: H-2b) were incubated with medium, 100 ng/mL LPS, 25 µg/mL poly I:C or 5 µg/mL Pam3CYS.
T125 18736-18908 Sentence denotes After 24 hours, cells were washed with PBS and incubated with freshly isolated allogeneic naïve CD4+ T cells (2.5×105 cells in 0.1 mL complete medium; MHC haplotype: H-2d).
T126 18909-19014 Sentence denotes After 5 days, supernatants were collected and the secretion of IFNγ, IL4, and IL2 were measured by ELISA.
T127 19015-19172 Sentence denotes Negative controls are 1) stimulated BMDC without co-culture with allogeneic CD4+ T cells; 2) allogeneic CD4+ T cells without co-culture with stimulated BMDC.
T128 19173-19282 Sentence denotes Samples from both negative controls had no detectable production of the aforementioned cytokines (not shown).
T129 19283-19342 Sentence denotes Results are analyzed based on 2–4 mice per group. * P<0.05.
T130 19344-19354 Sentence denotes Discussion
T131 19355-19536 Sentence denotes The present report showed that null mutations of the mouse Sharpin gene did not affect the steady-state distribution of splenic DC subsets nor the development and phenotype of BMDC.
T132 19537-19659 Sentence denotes However, loss of SHARPIN significantly diminished the capacity of BMDC to secrete inflammatory cytokines and nitric oxide.
T133 19660-19830 Sentence denotes The attenuated cytokine production was not due to the presence of anti-inflammatory inhibitors, and can be largely explained by selective inactivation of NF-κB signaling.
T134 19831-19970 Sentence denotes Stimulated cpdm BMDC exhibited Th2-biased T cell-polarizing capabilities, consistent with the Th2 cytokine-dominant phenotype in cpdm mice.
T135 19971-20152 Sentence denotes Together, these results indicate an indispensible role of SHARPIN in regulating DC immunological functions, disruption of which may contribute to the development of immune diseases.
T136 20153-20290 Sentence denotes Since WT and cpdm mice are both specific pathogen-free, the nature of the trigger of the severe inflammation in cpdm mice is not obvious.
T137 20291-20484 Sentence denotes One such initiating factor could be endogenous apoptotic and/or necrotic cells that can release danger-associated molecular patterns (DAMPs) to launch and amplify an inflammatory response [32].
T138 20485-20644 Sentence denotes Such ‘sterile’ inflammation could be initiated and take place in all organs and tissues affected in cpdm mice, thus causing multi-organ inflammatory disorders.
T139 20645-20700 Sentence denotes This hypothesis is supported by several recent studies.
T140 20701-20997 Sentence denotes Fibroblasts of cpdm mice are highly sensitive to TNFα-induced cell death and the cpdm phenotype can be partially rescued by deletion of TNF [7], [8], suggesting that deficiency of SHARPIN compromises the anti-apoptotic mechanisms in cpdm mice resulting in cell death-induced inflammatory disease.
T141 20998-21156 Sentence denotes Apoptosis of keratinocytes is a prominent feature of the skin lesions in cpdm mice [33] and this is mediated by caspase-dependent mitochondrial pathways [34].
T142 21157-21397 Sentence denotes These induced and/or intrinsic apoptotic cells can release various types of DAMPs that exert their pro-inflammatory properties by activating DC through pattern recognition receptors [32], [35], such as HMGB1 recognized by TLR2/4 [36], [37].
T143 21398-21580 Sentence denotes Consistent with the impaired Th1 immune response in cpdm mice [5], stimulated BMDC weakly polarized Th1 differentiation, but strongly supported the development of Th2 effector cells.
T144 21581-21786 Sentence denotes Combined with the dramatic effect of IL12 treatment on the phenotype of these mice [5], this suggests that the Th2-biased systemic inflammation in cpdm mice is caused by reduced IL12P70 production from DC.
T145 21787-21996 Sentence denotes The importance of IL12P70 in regulating Th1 and Th2 immune responses in mice was clearly demonstrated in IL12P35- and IL12P40-deficient mice [38], [39] and through clinical studies in human patients [40]–[42].
T146 21997-22160 Sentence denotes NF-κB, TBK/IRF3, and MAPK signaling are important pathways activated by LPS or poly I:C and disruption of either pathway may lead to decreased cytokine expression.
T147 22161-22429 Sentence denotes NF-κB activation was selectively inhibited in LPS and poly I:C stimulated cpdm BMDC compared to wild type, while activation of TBK1/IRF3, ERK1/2, or p38 was not, indicating that disrupted NF-κB signaling in cpdm DC is responsible for the defective cytokine expression.
T148 22430-22566 Sentence denotes These results seem to contradict a recent study that found increased levels of NF-κB activation and IL1 transcription in cpdm mice [43].
T149 22567-22738 Sentence denotes This difference can probably be attributed to the different cells and tissues used in these studies, and may point to cell- and tissue- type specific functions of SHARPIN.
T150 22739-22829 Sentence denotes This is consistent with recent reports of tissue-specific effects of NF-κB signaling [44].
T151 22830-23063 Sentence denotes Ubiquitous activation of NF-κB by removing inhibitors such as A20 and ITCH through genetic manipulation results in widespread inflammation, consistent with the role of NF-κB in the production of pro-inflammatory mediators [45], [46].
T152 23064-23248 Sentence denotes However, selective inhibition of NF-κB activation in parenchymal cells of the skin, liver, and intestine results in chronic inflammation driven by NF-κB competent leukocytes [47]–[50].
T153 23249-23441 Sentence denotes This indicates that a balance between the pro-inflammatory role of NF-κB in leukocytes and the anti-inflammatory role in parenchymal cells is critical in the maintenance of tissue homeostasis.
T154 23442-23596 Sentence denotes Experiments with mice with cell- and tissue-specific deletion of Sharpin, currently under way, will help to elucidate the role of SHARPIN in inflammation.
T155 23597-23778 Sentence denotes Despite defective NF-κB activation in the absence of Sharpin expression, there was no significant change in splenic DC populations or expression of co-stimulatory molecules on BMDC.
T156 23779-23982 Sentence denotes Different NF-κB subunits involved in the canonical and non-canonical branches of the NF-κB signaling pathway have distinct functions to control specific aspects of DC development and function [51], [52].
T157 23983-24227 Sentence denotes The non-canonical pathway (p100 processing to produce p52) appears to be intact in SHARPIN-deficient cells [7], [9] suggesting that the NF-κB heterodimer p52/RELB is sufficient to maintain the normal regulation of DC homeostasis and maturation.
T158 24228-24343 Sentence denotes The molecular basis by which the Sharpin mutation causes reduced NF-κB activation in BMDC remains to be determined.
T159 24344-24449 Sentence denotes LPS and poly I:C used here are well-defined ligands that specifically engage TLR4 and TLR3, respectively.
T160 24450-24641 Sentence denotes The expression profile of surface TLR4 complexes is similar between WT and cpdm BMDC suggesting that defective NF-κB activation is not a result of differential TLR expression on target cells.
T161 24642-24796 Sentence denotes LPS engages TLR4 to activate MYD88-dependent and TRIF-dependent pathways, whereas TLR3 stimulated by poly I:C only triggers TRIF-dependent signaling [53].
T162 24797-24990 Sentence denotes The defective NF-κB activation by both stimuli suggests that the Sharpin mutation interferes with the protein adaptors or kinases shared by both signaling pathways, such as RIP1 and TRAF6 [18].
T163 24991-25229 Sentence denotes Recent studies demonstrated that SHARPIN interacts with HOIP to form LUBAC that exerts its linear-ubiquitin-chain-ligase activity on NF-κB signaling players RIP1 and NEMO [7], an essential step for intact TNFα-stimulated NF-κB activation.
T164 25230-25347 Sentence denotes The Sharpin null mutation disrupts the ubiquitylation process and abrogates the TNFα-induced NF-κB signaling pathway.
T165 25348-25532 Sentence denotes Since TNFR and TLR partially share their downstream signaling cascades, a similar ubiquitin-mediated regulation may hold true for SHARPIN in LPS- and poly I:C-induced NF-κB activation.
T166 25533-25687 Sentence denotes In summary, the present study identified an indispensible role of SHARPIN in the production of pro-inflammatory mediators and TLR-induced NF-κB signaling.
T167 25688-25818 Sentence denotes The impaired Th1-stimulating ability of Sharpin-deficient DC may account for the Th2-dominant inflammatory phenotype of cpdm mice.
T168 25819-25902 Sentence denotes The balance between Th1 and Th2 differentiation is critical for immune homeostasis.
T169 25903-26127 Sentence denotes A better understanding of how such balance is maintained will help design cytokine treatment for human diseases with Th2-biased cytokine secretion similar to the mouse cpdm, such as allergies and hypereosinophilic syndromes.
T170 26129-26150 Sentence denotes Materials and Methods
T171 26152-26168 Sentence denotes Ethics Statement
T172 26169-26297 Sentence denotes All mouse work was carried out in strict accordance with protocols approved by the Institutional Animal Care and Use Committees.
T173 26299-26303 Sentence denotes Mice
T174 26304-26497 Sentence denotes Specific-pathogen free colonies of C57BL/KaLawRij-Sharpincpdm/RijSunJ (JR#7599) and WT mice were obtained from The Jackson Laboratory (Bar Harbor, ME) and were maintained in a barrier facility.
T175 26498-26558 Sentence denotes Normal littermate controls were either +/+ or +/Sharpincpdm.
T176 26559-26645 Sentence denotes These control animals were phenotypically indistinguishable and are referred to as WT.
T177 26646-26708 Sentence denotes Sex-matched WT and mutant mice were used at 6–10 weeks of age.
T178 26709-26890 Sentence denotes For some experiments, cpdm mice were crossed with transgenic mice with a bacterial artificial chromosome (BAC) containing the Sharpin gene (FVB/NJ-Tg(RP24-173I23)1Sun/Sun, JR#8279).
T179 26891-27029 Sentence denotes These mice were backcrossed onto the C57BL/KaLawRij-Sharpincpdm/RijSunJ background and N4 mice were used in the experiments reported here.
T180 27030-27161 Sentence denotes All mouse work was carried out in strict accordance with the approved protocols by the Institutional Animal Care and Use Committee.
T181 27163-27190 Sentence denotes Constructs and transfection
T182 27191-27298 Sentence denotes The complementary DNA (cDNA) of the mouse Sharpin gene was cloned and amplified from RAW264.7 RNA extracts.
T183 27299-27428 Sentence denotes The primer sequences were forward, 5′-CC ATG GCG ATG TCG CCG CCC GCC GGC GGT; reverse, 5′- AAG CTT CTA GGT GGA AGC TGC AGC AAG A.
T184 27429-27492 Sentence denotes The Sharpin cDNA was cloned into expression vector pFLAG-CMV-2.
T185 27493-27582 Sentence denotes Murine fibroblasts and macrophages were were used to express recombinant SHARPIN protein.
T186 27583-27663 Sentence denotes Cells (2×104) were seeded in 96-well treated plates the day before transfection.
T187 27664-27770 Sentence denotes After overnight incubation, 200 ng pFLAG-SHARPIN plasmids were transfected with 0.5 ul Lipofectamine 2000.
T188 27771-27834 Sentence denotes 24 hours later, cells were incubated with fresh culture medium.
T189 27835-27945 Sentence denotes After another 24 hours, cells were lysed to confirm the FLAG-SHARPIN expression by immunoblots with anti-FLAG.
T190 27946-28081 Sentence denotes Cells with no transfection and transfected with empty vector pFLAG-CMV-2 were used as negative control in all transfection experiments.
T191 28083-28101 Sentence denotes Generation of BMDC
T192 28102-28156 Sentence denotes BMDC were developed as previously reported [54], [55].
T193 28157-28206 Sentence denotes Cells were collected after 10–12 days of culture.
T194 28207-28263 Sentence denotes The cell yield was 2–3×107 cells/mouse with 80–95% BMDC.
T195 28265-28305 Sentence denotes Phenotype of BMDC and splenic DC subsets
T196 28306-28409 Sentence denotes The phenotype of BMDC was determined before and after 24 hour culture in the presence of 100 ng/mL LPS.
T197 28410-28816 Sentence denotes The cells were incubated in PBS with 0.1% NaN3, 1% BSA and 10% normal rabbit serum for 20 minutes on ice, washed and incubated for 30 minutes on ice with Alex Fluor-labeled anti-CD11c (MCD11C20, CALTAG) in combination with PE- anti-CD40 (3/23, BD Biosciences), PE-anti-CD80 (16-10A1, eBioscience), PE-anti-CD86 (P03.1, eBioscience), PE-anti-CD14 (Sa2.8, eBioscience) and biotinylated anti-TLR4 (BioLegend).
T198 28817-28939 Sentence denotes The cells incubated with biotinylated anti-TLR4/MD-2 were washed twice and incubated for 30 minutes on ice with avidin-PE.
T199 28940-29044 Sentence denotes To isolate splenic DC, spleens were collagenase digested and subject to Percoll gradient centrifugation.
T200 29045-29216 Sentence denotes The bands at the 35–55% interface were collected to stain with PE-anti-CD11c (HL3, BioLegend), APC-anti-PDCA-1 (927, BioLegend) and FITC-anti-CD8α (53–6.7, BD BioScience).
T201 29217-29309 Sentence denotes The cells were washed twice, fixed in 2% paraformaldehyde, and stored at 4°C until analysis.
T202 29310-29372 Sentence denotes Flow cytometry was performed in an Excel (Coulter) instrument.
T203 29373-29509 Sentence denotes Dead cells were omitted from the analysis by gating on forward and 90° light scatter, and 10,000 cells were analyzed by FlowJo software.
T204 29511-29543 Sentence denotes BMDC-T cell in vitro interaction
T205 29544-29640 Sentence denotes WT and cpdm BMDC (5×104) were stimulated with medium, 1 µg/mL LPS, 25 µg/mL and 5 µg/mL Pam3CYS.
T206 29641-29698 Sentence denotes 24 hours later, cells were collected and washed with PBS.
T207 29699-29877 Sentence denotes Allogeneic naïve CD4+ T cells were isolated from spleens of BALB/c mice by negative selection kit (Invitrogen) and were then added at 2.5×105 and co-cultured with activated BMDC.
T208 29878-29983 Sentence denotes After 5 days, supernatant was collected and the secretion of IFNγ, IL4, IL2, and IL17A measured by ELISA.
T209 29984-30141 Sentence denotes Negative controls are 1) stimulated BMDC without co-culture with allogeneic CD4+ T cells; 2) allogeneic CD4+ T cells without co-culture with stimulated BMDC.
T210 30142-30212 Sentence denotes Both negative controls show no production of aforementioned cytokines.
T211 30214-30236 Sentence denotes RNA expression by BMDC
T212 30237-30375 Sentence denotes BMDC were cultured at 106 cells/mL in 10 mL of RPMI-1640 complete medium in the presence or absence of 100 ng/mL LPS or 25 µg/mL poly I:C.
T213 30376-30484 Sentence denotes After 1 and 2 hours, RNA was isolated with TRI-reagent (Sigma) according to the manufacturer's instructions.
T214 30485-30567 Sentence denotes The expression of Il6, Il12p40, Gmcsf, and Sharpin mRNA was determined by qRT-PCR.
T215 30568-30626 Sentence denotes Primers and probes were purchased from Applied Biosystems.
T216 30627-30970 Sentence denotes Reverse transcription was performed at 42°C for 60 minutes with the final denaturation step at 90°C for 5 minutes in 30 µl containing 0.5 µg of total RNA. dNTPs, oligo(dT)15 primer, recombinant RNasin Ribonuclease Inhibitor, and M-MLV Reverse Transcriptase (all from Promega, Madison, WI) were used according to the manufacturer's instruction.
T217 30971-31304 Sentence denotes Reverse transcription was done in a PTC-200 Peltier Thermal Cycler (MJ Research, Watertown, MA). qRT-PCR was performed in ABI Prism 7700 Sequence Detection System with TaqMan® Gene Expression Assays (Applied Biosystems, Foster City, CA) for mouse Actb, IL6, IL12p40, Ifnβ, Gmcsf, and Sharpin according to the manufacturer's protocol.
T218 31305-31378 Sentence denotes The endogenous standard for normalization of the target gene was β-actin.
T219 31379-31448 Sentence denotes Relative gene expression was calculated using the 2−ΔΔCt method [56].
T220 31450-31485 Sentence denotes In vitro cytokine secretion by BMDC
T221 31486-31625 Sentence denotes The BMDC were cultured in triplicate wells of 24-well or 96-well plates at 106 or 105 cells/mL, respectively, in RPMI-1640 complete medium.
T222 31626-31703 Sentence denotes The cells were washed and stimulated with 100 ng/mL LPS or 25 µg/mL poly I:C.
T223 31704-31767 Sentence denotes After 24 hours, supernatants were harvested for ELISA analysis.
T224 31768-31852 Sentence denotes The presence of nitrite in the supernatants was determined using the Griess reagent.
T225 31854-31872 Sentence denotes Immunofluorescence
T226 31873-31941 Sentence denotes Fibroblast and macrophage cells were transfected with pFLAG-SHARPIN.
T227 31942-32085 Sentence denotes 48 hours later, cells were washed briefly with PBS, then fixed in cold methanol at −20°C for 10 minutes and cold acetone at -20°C for 1 minute.
T228 32086-32162 Sentence denotes Incubate cells with PBS+1% BSA for 15 minutes to block non-specific binding.
T229 32163-32308 Sentence denotes Fixed cells were then incubated with anti-FLAG (1∶100) as the primary antibody at room temperature for 1 hour, followed by three 5-minute washes.
T230 32309-32473 Sentence denotes Cells were further incubated with goat anti-rabbit IgG-FITC (1∶100) as the secondary antibody at room temperature for 30 minutes, followed by three 5-minute washes.
T231 32474-32537 Sentence denotes Cells were then examined using inverse fluorescence microscope.
T232 32539-32550 Sentence denotes Immunoblots
T233 32551-32694 Sentence denotes After adding stimulating ligands, 100 ng/mL LPS or 25 µg/mL poly I:C, BMDC were collected and lysed at 0-, 15-, 30-, and 60-minute time points.
T234 32695-32901 Sentence denotes Immunoblots were performed with antibodies (Cell Signaling Technology) against p-IKK1/2(#2697), p-IκBα (#9246), IκBα (#4814), p-TBK1(#5483), p-p38 (#9216), p38 (#9212), p-ERK1/2 (#4376), and ERK1/2 (#4695).
T235 32902-32978 Sentence denotes Beta-actin (sc-47778, Santa Cruz Biotechnology) was used as loading control.
T236 32980-33000 Sentence denotes Statistical analysis
T237 33001-33033 Sentence denotes Data are expressed as mean ± SD.
T238 33034-33150 Sentence denotes The statistical significance of differences of means between experimental groups was determined by Students' t-test.

ICD10

Id Subject Object Predicate Lexical cue
T723 373-383 http://purl.bioontology.org/ontology/ICD10/L30.9 denotes dermatitis
T2033 2375-2385 http://purl.bioontology.org/ontology/ICD10/L30.9 denotes dermatitis
T2034 2454-2464 http://purl.bioontology.org/ontology/ICD10/L30.9 denotes dermatitis
T2035 3072-3082 http://purl.bioontology.org/ontology/ICD10/L30.9 denotes dermatitis
T2036 3279-3289 http://purl.bioontology.org/ontology/ICD10/L30.9 denotes dermatitis
T2037 3635-3645 http://purl.bioontology.org/ontology/ICD10/L30.9 denotes dermatitis
T2711 5388-5398 http://purl.bioontology.org/ontology/ICD10/L30.9 denotes dermatitis
T9189 26085-26094 http://purl.bioontology.org/ontology/ICD10/T78.4 denotes allergies

events-check-again

Id Subject Object Predicate Lexical cue Negation Speculation
T831 1317-1323 Protein denotes MAPK11
T832 1324-1326 Protein denotes 12
T833 1327-1329 Protein denotes 13
T834 1330-1332 Protein denotes 14
T835 1363-1367 Protein denotes TBK1
T836 1592-1596 Negative_regulation denotes loss
T837 1600-1607 Protein denotes Sharpin
T2107 1771-1778 Protein denotes SHARPIN
T2108 1908-1917 Gene_expression denotes expressed
T2109 1970-1989 Negative_regulation denotes recessive mutations
T2110 1997-2004 Protein denotes Sharpin
T2111 2152-2161 Positive_regulation denotes resulting
T2112 2152-2161 Positive_regulation denotes resulting
T2113 2175-2186 Negative_regulation denotes termination
T2114 2195-2204 Transcription denotes synthesis
T2115 2209-2216 Negative_regulation denotes absence
T2116 2866-2875 Negative_regulation denotes decreased
T2117 2876-2885 Gene_expression denotes secretion
T2118 2889-2893 Protein denotes IFNγ
T2753 4755-4762 Protein denotes Sharpin
T2754 4777-4784 Protein denotes SHARPIN
T2755 4936-4943 Protein denotes SHARPIN
T2756 5150-5157 Protein denotes SHARPIN
T2757 5214-5221 Protein denotes SHARPIN
T2758 5284-5293 Negative_regulation denotes mutations
T2759 5307-5314 Protein denotes Sharpin
T2760 5524-5534 Transcription denotes expression
T2761 5538-5545 Protein denotes Sharpin
T2762 5678-5685 Protein denotes Sharpin
T2763 5695-5702 Transcription denotes present
T2764 5771-5780 Negative_regulation denotes decreased
T2765 5825-5834 Negative_regulation denotes reduction
T2766 5838-5845 Protein denotes Sharpin
T2767 5924-5931 Protein denotes Sharpin
T2768 5993-6004 Entity denotes cytoplasmic
T2769 6005-6017 Localization denotes localization
T2770 6025-6032 Protein denotes SHARPIN
T3733 7401-7408 Protein denotes Sharpin
T3734 7409-7417 Negative_regulation denotes mutation
T3735 7536-7541 Protein denotes CD11c
T3736 7542-7546 Protein denotes CD8α
T3737 7552-7557 Protein denotes CD11c
T3738 7558-7562 Protein denotes CD8α
T3739 7596-7601 Protein denotes CD11c
T3740 7602-7608 Protein denotes PDCA-1
T3741 7827-7834 Protein denotes SHARPIN
T3742 7835-7845 Negative_regulation denotes deficiency
T3743 8162-8169 Protein denotes Sharpin
T3744 8170-8178 Negative_regulation denotes mutation
T3745 9763-9768 Protein denotes CD11c
T3746 9830-9834 Protein denotes CD40
T3747 9836-9840 Protein denotes CD80
T3748 9846-9850 Protein denotes CD86
T3749 9856-9860 Protein denotes TLR3
T3750 9861-9867 Binding denotes ligand
T3751 9885-9889 Protein denotes TLR4
T3752 9890-9896 Binding denotes ligand
T3753 10056-10064 Positive_regulation denotes resulted
T3754 10056-10064 Positive_regulation denotes resulted
T3755 10056-10064 Positive_regulation denotes resulted
T3756 10068-10077 Positive_regulation denotes increased
T3757 10068-10077 Positive_regulation denotes increased
T3758 10068-10077 Positive_regulation denotes increased
T3759 10078-10088 Gene_expression denotes expression
T3760 10078-10088 Gene_expression denotes expression
T3761 10078-10088 Gene_expression denotes expression
T3762 10092-10096 Protein denotes CD40
T3763 10098-10102 Protein denotes CD80
T3764 10108-10112 Protein denotes CD86
T3765 10144-10154 Regulation denotes difference true
T3766 10144-10154 Regulation denotes difference true
T3767 10144-10154 Regulation denotes difference true
T3768 10162-10172 Gene_expression denotes expression
T3769 10162-10172 Gene_expression denotes expression
T3770 10162-10172 Gene_expression denotes expression
T3771 10239-10246 Protein denotes SHARPIN
T3772 10247-10257 Negative_regulation denotes deficiency
T4247 10426-10434 Positive_regulation denotes resulted
T4248 10426-10434 Positive_regulation denotes resulted
T4249 10438-10447 Gene_expression denotes secretion
T4250 10438-10447 Gene_expression denotes secretion
T4251 10451-10454 Protein denotes IL6
T4252 10469-10474 Protein denotes GMCSF
T4253 10532-10540 Gene_expression denotes produced
T4254 10532-10540 Gene_expression denotes produced
T4255 10555-10559 Positive_regulation denotes less true
T4256 10555-10559 Positive_regulation denotes less true
T4257 10815-10822 Protein denotes Sharpin
T4258 10823-10832 Negative_regulation denotes deficient
T4259 10870-10877 Protein denotes Sharpin
T4260 11091-11098 Protein denotes SHARPIN
T4261 11195-11198 Protein denotes Il6
T4262 11200-11207 Protein denotes Il12p40
T4263 11209-11214 Protein denotes Gmcsf
T4264 11220-11224 Protein denotes Ifnb
T5568 13365-13369 Protein denotes TLR4
T5569 13394-13398 Protein denotes CD14
T5570 13403-13435 Protein denotes myeloid differentiation factor 2
T5571 13437-13440 Protein denotes MD2
T5572 13441-13445 Protein denotes LY96
T5573 13493-13503 Gene_expression denotes expression
T5574 13493-13503 Gene_expression denotes expression
T5575 13493-13503 Gene_expression denotes expression
T5576 13514-13518 Protein denotes CD14
T5577 13523-13527 Protein denotes TLR4
T5578 13528-13531 Protein denotes MD2
T5579 13604-13613 Gene_expression denotes secretion true
T5580 13643-13647 Protein denotes IL10
T5581 13786-13791 Positive_regulation denotes lower true
T5582 13792-13798 Gene_expression denotes levels
T5583 13802-13806 Protein denotes IL10
T5584 13858-13862 Protein denotes IL10
T5585 13932-13941 Positive_regulation denotes Increased
T5586 13942-13952 Gene_expression denotes expression
T5587 14002-14005 Protein denotes A20
T5588 14062-14078 Transcription denotes transcript level
T5589 14082-14085 Protein denotes A20
T5590 14090-14095 Positive_regulation denotes lower true
T5591 14170-14184 Positive_regulation denotes overexpression
T5592 14188-14191 Protein denotes A20
T5593 15035-15039 Protein denotes TLR3
T5594 15040-15041 Protein denotes 4
T5595 15154-15158 Protein denotes TBK1
T5596 15159-15163 Protein denotes IRF3
T5597 15224-15228 Protein denotes TBK1
T5598 15229-15233 Protein denotes IRF3
T5599 15319-15326 Protein denotes Sharpin
T5600 15327-15336 Negative_regulation denotes deficient
T5601 15395-15399 Protein denotes IKK1
T5602 15400-15401 Protein denotes 2
T5603 15444-15452 Positive_regulation denotes allowing
T5604 15444-15452 Positive_regulation denotes allowing
T5605 15453-15468 Phosphorylation denotes phosphorylation
T5606 15484-15492 Positive_regulation denotes mediated
T5607 15493-15504 Protein_catabolism denotes degradation
T5608 15528-15532 Protein denotes IκBα
T5609 15608-15622 Phosphorylation denotes phosphorylated
T5610 15608-15622 Phosphorylation denotes phosphorylated
T5611 15608-15622 Phosphorylation denotes phosphorylated
T5612 15623-15627 Protein denotes IKK1
T5613 15628-15629 Protein denotes 2
T5614 15633-15637 Protein denotes IKK1
T5615 15638-15639 Protein denotes 2
T5616 15645-15649 Protein denotes IκBα
T5617 15653-15657 Protein denotes IκBα
T5618 15714-15723 Negative_regulation denotes decreased
T5619 15714-15723 Negative_regulation denotes decreased
T5620 15714-15723 Negative_regulation denotes decreased
T5621 15818-15822 Protein denotes TBK1
T5622 15824-15828 Protein denotes ERK1
T5623 15829-15830 Protein denotes 2
T5624 15840-15855 Phosphorylation denotes phosphorylation
T5625 15840-15855 Phosphorylation denotes phosphorylation
T5626 15840-15855 Phosphorylation denotes phosphorylation
T5627 15919-15926 Negative_regulation denotes absence
T5628 15941-15948 Protein denotes SHARPIN
T5629 15995-15999 Protein denotes TBK1
T5630 16000-16004 Protein denotes IRF3
T5631 16006-16010 Protein denotes ERK1
T5632 16011-16012 Protein denotes 2
T6522 16851-16858 Negative_regulation denotes absence
T6523 16862-16869 Protein denotes SHARPIN
T6524 17046-17054 Positive_regulation denotes elicited
T6525 17062-17066 Protein denotes IFNγ
T6526 17067-17077 Gene_expression denotes production
T6527 17091-17104 Gene_expression denotes concentration
T6528 17108-17112 Protein denotes IFNγ
T6529 17160-17165 Positive_regulation denotes lower true
T6530 17273-17277 Protein denotes TLR3
T6531 17278-17279 Protein denotes 4
T6532 17280-17288 Positive_regulation denotes agonists
T6533 17280-17288 Positive_regulation denotes agonists
T6534 17294-17298 Protein denotes TLR2
T6535 17299-17305 Binding denotes ligand
T6536 17306-17313 Protein denotes Pam3CYS
T6537 17395-17402 Protein denotes Pam3CYS
T6538 17419-17426 Positive_regulation denotes induced
T6539 17434-17438 Protein denotes IFNγ
T6540 17439-17449 Gene_expression denotes production
T6541 17550-17557 Protein denotes Pam3CYS
T6542 17666-17670 Protein denotes TLR2
T6543 17720-17723 Protein denotes IL4
T6544 17737-17745 Gene_expression denotes produced
T6545 17861-17871 Gene_expression denotes production
T6546 17898-17903 Protein denotes IL17A
T6547 17904-17913 Positive_regulation denotes following
T6548 18134-18137 Protein denotes IL2
T6549 18138-18148 Gene_expression denotes production
T6550 18224-18231 Positive_regulation denotes induced
T6551 18232-18236 Positive_regulation denotes more
T6552 18237-18240 Protein denotes IL2
T9371 19386-19400 Negative_regulation denotes null mutations
T9372 19414-19421 Protein denotes Sharpin
T9373 19546-19550 Negative_regulation denotes loss
T9374 19554-19561 Protein denotes SHARPIN
T9375 20029-20036 Protein denotes SHARPIN
T9376 20079-20089 Negative_regulation denotes disruption
T9377 20750-20754 Protein denotes TNFα
T9378 20825-20833 Negative_regulation denotes deletion
T9379 20837-20840 Protein denotes TNF
T9380 20867-20877 Negative_regulation denotes deficiency
T9381 20881-20888 Protein denotes SHARPIN
T9382 21359-21364 Protein denotes HMGB1
T9383 21365-21375 Binding denotes recognized
T9384 21379-21383 Protein denotes TLR2
T9385 21384-21385 Protein denotes 4
T9386 21892-21899 Protein denotes IL12P35
T9387 21905-21912 Protein denotes IL12P40
T9388 21913-21922 Negative_regulation denotes deficient
T9389 21913-21922 Negative_regulation denotes deficient
T9390 22008-22012 Protein denotes IRF3
T9391 22274-22284 Positive_regulation denotes activation
T9392 22274-22284 Positive_regulation denotes activation
T9393 22274-22284 Positive_regulation denotes activation
T9394 22274-22284 Positive_regulation denotes activation
T9395 22288-22292 Protein denotes TBK1
T9396 22293-22297 Protein denotes IRF3
T9397 22299-22303 Protein denotes ERK1
T9398 22304-22305 Protein denotes 2
T9399 22314-22321 Negative_regulation denotes was not true
T9400 22314-22321 Negative_regulation denotes was not true
T9401 22314-22321 Negative_regulation denotes was not true
T9402 22314-22321 Negative_regulation denotes was not true
T9403 22730-22737 Protein denotes SHARPIN
T9404 22892-22895 Protein denotes A20
T9405 22900-22904 Protein denotes ITCH
T9406 23495-23503 Negative_regulation denotes deletion
T9407 23507-23514 Protein denotes Sharpin
T9408 23572-23579 Protein denotes SHARPIN
T9409 23639-23646 Negative_regulation denotes absence
T9410 23650-23657 Protein denotes Sharpin
T9411 23658-23668 Gene_expression denotes expression
T9412 24010-24014 Protein denotes p100
T9413 24037-24040 Protein denotes p52
T9414 24066-24073 Protein denotes SHARPIN
T9415 24074-24083 Negative_regulation denotes deficient
T9416 24137-24140 Protein denotes p52
T9417 24141-24145 Protein denotes RELB
T9418 24261-24268 Protein denotes Sharpin
T9419 24269-24277 Negative_regulation denotes mutation
T9420 24414-24420 Binding denotes engage
T9421 24414-24420 Binding denotes engage
T9422 24421-24425 Protein denotes TLR4
T9423 24430-24434 Protein denotes TLR3
T9424 24646-24653 Binding denotes engages
T9425 24654-24658 Protein denotes TLR4
T9426 24671-24676 Protein denotes MYD88
T9427 24691-24695 Protein denotes TRIF
T9428 24724-24728 Protein denotes TLR3
T9429 24729-24739 Positive_regulation denotes stimulated
T9430 24766-24770 Protein denotes TRIF
T9431 24862-24869 Protein denotes Sharpin
T9432 24870-24878 Negative_regulation denotes mutation
T9433 24970-24974 Protein denotes RIP1
T9434 24979-24984 Protein denotes TRAF6
T9435 25024-25031 Protein denotes SHARPIN
T9436 25032-25041 Binding denotes interacts
T9437 25047-25051 Protein denotes HOIP
T9438 25071-25077 Positive_regulation denotes exerts
T9439 25071-25077 Positive_regulation denotes exerts
T9440 25082-25120 Ubiquitination denotes linear-ubiquitin-chain-ligase activity
T9441 25082-25120 Ubiquitination denotes linear-ubiquitin-chain-ligase activity
T9442 25148-25152 Protein denotes RIP1
T9443 25157-25161 Protein denotes NEMO
T9444 25196-25200 Protein denotes TNFα
T9445 25234-25241 Protein denotes Sharpin
T9446 25242-25255 Negative_regulation denotes null mutation
T9447 25256-25264 Negative_regulation denotes disrupts
T9448 25256-25264 Negative_regulation denotes disrupts
T9449 25310-25314 Protein denotes TNFα
T9450 25478-25485 Protein denotes SHARPIN
T9451 25599-25606 Protein denotes SHARPIN
T9452 25728-25735 Protein denotes Sharpin
T9453 25736-25745 Negative_regulation denotes deficient
T9936 26835-26842 Protein denotes Sharpin
T10316 27233-27240 Protein denotes Sharpin
T10317 27433-27440 Protein denotes Sharpin
T10318 27546-27553 Gene_expression denotes express
T10319 27566-27573 Protein denotes SHARPIN
T10320 27705-27712 Protein denotes SHARPIN
T10321 27896-27903 Protein denotes SHARPIN
T10322 27904-27914 Gene_expression denotes expression true
T11284 29926-29935 Gene_expression denotes secretion true
T11285 29926-29935 Gene_expression denotes secretion true
T11286 29926-29935 Gene_expression denotes secretion true
T11287 29926-29935 Gene_expression denotes secretion true
T11288 29939-29943 Protein denotes IFNγ
T11289 29945-29948 Protein denotes IL4
T11290 29950-29953 Protein denotes IL2
T11291 29959-29964 Protein denotes IL17A
T11292 30173-30183 Gene_expression denotes production true
T11293 30173-30183 Gene_expression denotes production true
T11294 30173-30183 Gene_expression denotes production true
T11295 30173-30183 Gene_expression denotes production true
T11890 30489-30499 Transcription denotes expression true
T11891 30489-30499 Transcription denotes expression true
T11892 30489-30499 Transcription denotes expression true
T11893 30489-30499 Transcription denotes expression true
T11894 30503-30506 Protein denotes Il6
T11895 30508-30515 Protein denotes Il12p40
T11896 30517-30522 Protein denotes Gmcsf
T11897 30528-30535 Protein denotes Sharpin
T11898 30856-30883 Protein denotes M-MLV Reverse Transcriptase
T11899 31218-31222 Protein denotes Actb
T11900 31224-31227 Protein denotes IL6
T11901 31229-31236 Protein denotes IL12p40
T11902 31238-31242 Protein denotes Ifnβ
T11903 31244-31249 Protein denotes Gmcsf
T11904 31255-31262 Protein denotes Sharpin
T11905 31370-31377 Protein denotes β-actin
T12351 31933-31940 Protein denotes SHARPIN
T12662 32776-32780 Protein denotes IKK1
T12663 32781-32782 Protein denotes 2
T12664 32793-32797 Protein denotes IκBα
T12665 32807-32811 Protein denotes IκBα
T12666 32823-32827 Protein denotes TBK1
T12667 32866-32870 Protein denotes ERK1
T12668 32871-32872 Protein denotes 2
T12669 32886-32890 Protein denotes ERK1
T12670 32891-32892 Protein denotes 2
T12671 32902-32912 Protein denotes Beta-actin
T13238 6129-6136 Protein denotes SHARPIN
T13239 6355-6362 Protein denotes SHARPIN
T13240 6716-6726 Transcription denotes mRNA level true
T13241 6730-6737 Protein denotes Sharpin
T13242 7007-7014 Protein denotes SHARPIN
T13243 7183-7190 Protein denotes SHARPIN
T13244 7207-7216 Entity denotes cytoplasm
T13245 7217-7226 Localization denotes localized
T13792 8308-8315 Protein denotes Sharpin
T13793 8316-8324 Negative_regulation denotes mutation
T14222 11679-11682 Protein denotes IL6
T14223 11688-11693 Protein denotes GMCSF
T14224 11912-11915 Protein denotes IL6
T14225 11981-11988 Protein denotes Sharpin
T14530 12685-12695 Transcription denotes expression true
T14531 12685-12695 Transcription denotes expression true
T14532 12685-12695 Transcription denotes expression true
T14533 12685-12695 Transcription denotes expression true
T14534 12699-12706 Protein denotes Il12p40
T14535 12714-12717 Protein denotes Il6
T14536 12725-12730 Protein denotes Gmcsf
T14537 12742-12746 Protein denotes Ifnb
T14938 14284-14290 Negative_regulation denotes Normal true
T14939 14284-14290 Negative_regulation denotes Normal true
T14940 14291-14295 Protein denotes TLR4
T14941 14300-14303 Protein denotes MD2
T14942 14304-14314 Gene_expression denotes expression
T14943 14304-14314 Gene_expression denotes expression
T14944 14319-14328 Negative_regulation denotes decreased
T14945 14319-14328 Negative_regulation denotes decreased
T14946 14329-14333 Protein denotes IL10
T14947 14334-14343 Gene_expression denotes secretion
T14948 14348-14351 Protein denotes A20
T14949 14352-14362 Gene_expression denotes expression
T14950 14764-14768 Protein denotes IL10
T14951 14992-15002 Gene_expression denotes production true
T14952 15006-15009 Protein denotes A20
T15295 16393-16397 Protein denotes TBK1
T15296 16398-16402 Protein denotes IRF3
T15297 16404-16408 Protein denotes ERK1
T15298 16409-16410 Protein denotes 2
T15299 16440-16450 Protein denotes Beta-actin
T15300 16505-16509 Protein denotes IKK1
T15301 16510-16511 Protein denotes 2
T15302 16518-16522 Protein denotes IκBα
T15724 18959-18968 Gene_expression denotes secretion true
T15725 18959-18968 Gene_expression denotes secretion true
T15726 18959-18968 Gene_expression denotes secretion true
T15727 18972-18976 Protein denotes IFNγ
T15728 18978-18981 Protein denotes IL4
T15729 18987-18990 Protein denotes IL2
T15730 19227-19237 Gene_expression denotes production true
T15731 19227-19237 Gene_expression denotes production true
T15732 19227-19237 Gene_expression denotes production true
T802 0-7 Protein denotes SHARPIN
T830 1310-1311 Protein denotes 2
T803 177-186 Negative_regulation denotes mutations
T804 194-201 Protein denotes Sharpin
T805 203-249 Protein denotes SHANK-associated RH domain-interacting protein
T806 266-272 Protein denotes Rbckl1
T807 274-279 Protein denotes Sipl1
T808 627-634 Protein denotes SHARPIN
T809 635-644 Negative_regulation denotes deficient
T810 749-756 Protein denotes SHARPIN
T811 758-768 Negative_regulation denotes deficiency
T812 1053-1057 Protein denotes CD40
T813 1059-1063 Protein denotes CD80
T814 1069-1073 Protein denotes CD86
T815 1095-1102 Protein denotes SHARPIN
T816 1103-1112 Negative_regulation denotes deficient
T817 1122-1129 Negative_regulation denotes reduced
T818 1122-1129 Negative_regulation denotes reduced
T819 1122-1129 Negative_regulation denotes reduced
T820 1122-1129 Negative_regulation denotes reduced
T821 1130-1143 Transcription denotes transcription
T822 1130-1143 Transcription denotes transcription
T823 1148-1157 Gene_expression denotes secretion
T824 1148-1157 Gene_expression denotes secretion
T825 1188-1191 Protein denotes IL6
T826 1202-1207 Protein denotes GMCSF
T827 1296-1301 Protein denotes MAPK1
T828 1302-1303 Protein denotes 3
T829 1305-1309 Protein denotes ERK1
T2119 3088-3102 Negative_regulation denotes Neutralization
T2120 3106-3109 Protein denotes IL5
T2121 3148-3151 Protein denotes IL5
T2122 3152-3161 Negative_regulation denotes deficient
T2123 3341-3348 Protein denotes SHARPIN
T2124 3445-3449 Protein denotes TNFα
T2125 3493-3500 Protein denotes SHARPIN
T2126 3501-3510 Negative_regulation denotes deficient
T2127 3563-3567 Protein denotes TNFα
T2128 4215-4222 Protein denotes SHARPIN
T2129 4223-4232 Negative_regulation denotes deficient
T2130 4276-4280 Negative_regulation denotes lack
T2131 4284-4291 Protein denotes SHARPIN
T2132 4569-4576 Protein denotes SHARPIN
T2133 4668-4675 Protein denotes SHARPIN
T2134 4676-4685 Negative_regulation denotes deficient
R7734 T9372 T9371 themeOf Sharpin,null mutations
R7735 T9374 T9373 themeOf SHARPIN,loss
R7736 T9375 T9376 themeOf SHARPIN,disruption
R7739 T9379 T9378 themeOf TNF,deletion
R7740 T9381 T9380 themeOf SHARPIN,deficiency
R7741 T9382 T9383 themeOf HMGB1,recognized
R7743 T9384 T9383 themeOf TLR2,recognized
R7744 T9386 T9389 themeOf IL12P35,deficient
R7745 T9387 T9388 themeOf IL12P40,deficient
R7747 T9391 T9400 themeOf activation,was not
R7748 T9392 T9402 themeOf activation,was not
R7749 T9393 T9399 themeOf activation,was not
R7750 T9394 T9401 themeOf activation,was not
R7751 T9395 T9394 themeOf TBK1,activation
R7753 T9396 T9393 themeOf IRF3,activation
R7754 T9397 T9391 themeOf ERK1,activation
R7755 T9398 T9392 themeOf 2,activation
R7759 T9407 T9406 themeOf Sharpin,deletion
R7761 T9410 T9411 themeOf Sharpin,expression
R7762 T9411 T9409 themeOf expression,absence
R7764 T9414 T9415 themeOf SHARPIN,deficient
R7767 T9418 T9419 themeOf Sharpin,mutation
R7769 T9422 T9420 themeOf TLR4,engage
R7770 T9423 T9421 themeOf TLR3,engage
R7772 T9425 T9424 themeOf TLR4,engages
R7775 T9428 T9429 themeOf TLR3,stimulated
R7777 T9431 T9432 themeOf Sharpin,mutation
R7780 T9435 T9436 themeOf SHARPIN,interacts
R7781 T9435 T9440 causeOf SHARPIN,linear-ubiquitin-chain-ligase activity
R7782 T9435 T9441 causeOf SHARPIN,linear-ubiquitin-chain-ligase activity
R7783 T9437 T9436 themeOf HOIP,interacts
R7785 T9440 T9439 themeOf linear-ubiquitin-chain-ligase activity,exerts
R7786 T9440 T9447 themeOf linear-ubiquitin-chain-ligase activity,disrupts
R7788 T9441 T9438 themeOf linear-ubiquitin-chain-ligase activity,exerts
R7789 T9441 T9448 themeOf linear-ubiquitin-chain-ligase activity,disrupts
R7791 T9442 T9440 themeOf RIP1,linear-ubiquitin-chain-ligase activity
R7792 T9443 T9441 themeOf NEMO,linear-ubiquitin-chain-ligase activity
R7793 T9445 T9446 themeOf Sharpin,null mutation
R7794 T9446 T9447 causeOf null mutation,disrupts
R7795 T9446 T9448 causeOf null mutation,disrupts
R7797 T9452 T9453 themeOf Sharpin,deficient
R1758 T2109 T2112 causeOf recessive mutations,resulting
R1762 T2110 T2115 themeOf Sharpin,absence
R1763 T2110 T2114 themeOf Sharpin,synthesis
R1767 T2115 T2112 themeOf absence,resulting
R1768 T2120 T2119 themeOf IL5,Neutralization
R1769 T2117 T2116 themeOf secretion,decreased
R1770 T2121 T2122 themeOf IL5,deficient
R1771 T2118 T2117 themeOf IFNγ,secretion
R1772 T2125 T2126 themeOf SHARPIN,deficient
R1774 T2128 T2129 themeOf SHARPIN,deficient
R1775 T2131 T2130 themeOf SHARPIN,lack
R2277 T2759 T2758 themeOf Sharpin,mutations
R2278 T2761 T2760 themeOf Sharpin,expression
R2279 T2762 T2763 themeOf Sharpin,present
R2280 T2763 T2764 themeOf present,decreased
R2281 T2766 T2765 themeOf Sharpin,reduction
R2282 T2768 T2769 locationOf cytoplasmic,localization
R2283 T2770 T2769 themeOf SHARPIN,localization
R3092 T3733 T3734 themeOf Sharpin,mutation
R3093 T3741 T3742 themeOf SHARPIN,deficiency
R3094 T3743 T3744 themeOf Sharpin,mutation
R3095 T3749 T3750 themeOf TLR3,ligand
R3096 T3751 T3752 themeOf TLR4,ligand
R3097 T3756 T3753 themeOf increased,resulted
R3098 T3757 T3754 themeOf increased,resulted
R3099 T3758 T3755 themeOf increased,resulted
R3100 T3759 T3757 themeOf expression,increased
R3101 T3760 T3758 themeOf expression,increased
R3102 T3761 T3756 themeOf expression,increased
R3103 T3762 T3759 themeOf CD40,expression
R3104 T3762 T3768 themeOf CD40,expression
R3105 T3763 T3760 themeOf CD80,expression
R3106 T3763 T3769 themeOf CD80,expression
R3107 T3764 T3761 themeOf CD86,expression
R3108 T3764 T3770 themeOf CD86,expression
R3109 T3768 T3765 themeOf expression,difference
R3110 T3769 T3766 themeOf expression,difference
R3111 T3770 T3767 themeOf expression,difference
R3112 T3771 T3772 themeOf SHARPIN,deficiency
R3521 T4249 T4248 themeOf secretion,resulted
R3522 T4250 T4247 themeOf secretion,resulted
R3523 T4251 T4250 themeOf IL6,secretion
R3524 T4251 T4253 themeOf IL6,produced
R3525 T4252 T4249 themeOf GMCSF,secretion
R3526 T4252 T4254 themeOf GMCSF,produced
R3527 T4253 T4255 themeOf produced,less
R3528 T4254 T4256 themeOf produced,less
R3529 T4257 T4258 themeOf Sharpin,deficient
R4513 T5571 T5570 equivalentTo MD2,myeloid differentiation factor 2
R4514 T5572 T5570 equivalentTo LY96,myeloid differentiation factor 2
R4515 T5576 T5574 themeOf CD14,expression
R4516 T5577 T5575 themeOf TLR4,expression
R4517 T5578 T5573 themeOf MD2,expression
R4518 T5580 T5579 themeOf IL10,secretion
R4519 T5582 T5581 themeOf levels,lower
R4520 T5583 T5582 themeOf IL10,levels
R4521 T5586 T5585 themeOf expression,Increased
R4522 T5587 T5586 themeOf A20,expression
R4523 T5588 T5590 themeOf transcript level,lower
R4524 T5589 T5588 themeOf A20,transcript level
R4525 T5592 T5591 themeOf A20,overexpression
R4526 T5599 T5600 themeOf Sharpin,deficient
R4527 T5605 T5604 themeOf phosphorylation,allowing
R4528 T5607 T5603 themeOf degradation,allowing
R4529 T5607 T5606 themeOf degradation,mediated
R4530 T5608 T5605 themeOf IκBα,phosphorylation
R4531 T5608 T5607 themeOf IκBα,degradation
R4532 T5609 T5618 themeOf phosphorylated,decreased
R4533 T5610 T5620 themeOf phosphorylated,decreased
R4534 T5611 T5619 themeOf phosphorylated,decreased
R4535 T5612 T5610 themeOf IKK1,phosphorylated
R4536 T5613 T5609 themeOf 2,phosphorylated
R4537 T5616 T5611 themeOf IκBα,phosphorylated
R4538 T5621 T5626 themeOf TBK1,phosphorylation
R4539 T5622 T5624 themeOf ERK1,phosphorylation
R4540 T5623 T5625 themeOf 2,phosphorylation
R4541 T5628 T5627 themeOf SHARPIN,absence
R5295 T6523 T6522 themeOf SHARPIN,absence
R5296 T6525 T6526 themeOf IFNγ,production
R5297 T6526 T6524 themeOf production,elicited
R5298 T6527 T6529 themeOf concentration,lower
R5299 T6528 T6527 themeOf IFNγ,concentration
R5300 T6530 T6533 themeOf TLR3,agonists
R5301 T6531 T6532 themeOf 4,agonists
R5302 T6534 T6535 themeOf TLR2,ligand
R5303 T6536 T6535 themeOf Pam3CYS,ligand
R5304 T6537 T6538 causeOf Pam3CYS,induced
R5305 T6539 T6540 themeOf IFNγ,production
R5306 T6540 T6538 themeOf production,induced
R5307 T6543 T6544 themeOf IL4,produced
R5308 T6545 T6547 themeOf production,following
R5309 T6546 T6545 themeOf IL17A,production
R5310 T6548 T6549 themeOf IL2,production
R5311 T6550 T6551 themeOf induced,more
R5312 T6552 T6550 themeOf IL2,induced
R8505 T10319 T10318 themeOf SHARPIN,express
R8506 T10321 T10322 themeOf SHARPIN,expression
R9877 T11894 T11893 themeOf Il6,expression
R9878 T11895 T11892 themeOf Il12p40,expression
R9879 T11896 T11891 themeOf Gmcsf,expression
R9880 T11897 T11890 themeOf Sharpin,expression
R11497 T13792 T13793 themeOf Sharpin,mutation
R12159 T14534 T14533 themeOf Il12p40,expression
R12160 T14535 T14532 themeOf Il6,expression
R12161 T14536 T14531 themeOf Gmcsf,expression
R12162 T14537 T14530 themeOf Ifnb,expression
R12521 T14940 T14943 themeOf TLR4,expression
R12522 T14941 T14942 themeOf MD2,expression
R12523 T14942 T14939 themeOf expression,Normal
R12524 T14943 T14938 themeOf expression,Normal
R12525 T14946 T14947 themeOf IL10,secretion
R12526 T14947 T14944 themeOf secretion,decreased
R12527 T14948 T14949 themeOf A20,expression
R12528 T14949 T14945 themeOf expression,decreased
R12529 T14952 T14951 themeOf A20,production
R13160 T15727 T15726 themeOf IFNγ,secretion
R13161 T15727 T15730 themeOf IFNγ,production
R13162 T15728 T15724 themeOf IL4,secretion
R13163 T15728 T15731 themeOf IL4,production
R13164 T15729 T15732 themeOf IL2,production
R13165 T15729 T15725 themeOf IL2,secretion
R711 T826 T822 themeOf GMCSF,transcription
R712 T826 T823 themeOf GMCSF,secretion
R713 T829 T827 equivalentTo ERK1,MAPK1
R714 T830 T828 equivalentTo 2,3
R629 T837 T836 themeOf Sharpin,loss
R694 T804 T803 themeOf Sharpin,mutations
R695 T805 T804 equivalentTo SHANK-associated RH domain-interacting protein,Sharpin
R696 T806 T804 equivalentTo Rbckl1,Sharpin
R697 T807 T804 equivalentTo Sipl1,Sharpin
R698 T808 T809 themeOf SHARPIN,deficient
R699 T810 T811 themeOf SHARPIN,deficiency
R700 T815 T816 themeOf SHARPIN,deficient
R701 T816 T818 causeOf deficient,reduced
R702 T816 T820 causeOf deficient,reduced
R703 T816 T819 causeOf deficient,reduced
R704 T816 T817 causeOf deficient,reduced
R705 T821 T818 themeOf transcription,reduced
R706 T822 T820 themeOf transcription,reduced
R707 T823 T817 themeOf secretion,reduced
R708 T824 T819 themeOf secretion,reduced
R709 T825 T821 themeOf IL6,transcription
R710 T825 T824 themeOf IL6,secretion
R9372 T11288 T11292 themeOf IFNγ,production
R9373 T11288 T11287 themeOf IFNγ,secretion
R9374 T11289 T11293 themeOf IL4,production
R9375 T11289 T11286 themeOf IL4,secretion
R9376 T11290 T11294 themeOf IL2,production
R9377 T11290 T11284 themeOf IL2,secretion
R9378 T11291 T11295 themeOf IL17A,production
R9379 T11291 T11285 themeOf IL17A,secretion
R11000 T13241 T13240 themeOf Sharpin,mRNA level
R11001 T13243 T13245 themeOf SHARPIN,localized
R11002 T13244 T13245 locationOf cytoplasm,localized
R1757 T2107 T2108 themeOf SHARPIN,expressed
R1761 T2109 T2111 causeOf recessive mutations,resulting
R1764 T2110 T2109 themeOf Sharpin,recessive mutations
R1765 T2113 T2111 themeOf termination,resulting
R1766 T2114 T2113 themeOf synthesis,termination
R1777 T2133 T2134 themeOf SHARPIN,deficient

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T838 0-7 Protein denotes SHARPIN
T839 46-51 Protein denotes NF-κB
T840 194-201 Protein denotes Sharpin
T841 203-208 Protein denotes SHANK
T842 220-222 Protein denotes RH
T843 266-272 Protein denotes Rbckl1
T844 274-279 Protein denotes Sipl1
T845 414-423 Protein denotes T helper1
T846 425-428 Protein denotes Th1
T847 177-186 Gene_expression denotes mutations
T848 177-186 Gene_expression denotes mutations
T849 177-186 Gene_expression denotes mutations
T850 177-186 Gene_expression denotes mutations
T851 209-219 Gene_expression denotes associated
T852 209-219 Gene_expression denotes associated
T853 209-219 Gene_expression denotes associated
T854 209-219 Gene_expression denotes associated
T855 401-410 Localization denotes secretion
T856 401-410 Localization denotes secretion
T857 388-400 Regulation denotes dysregulated
T858 388-400 Regulation denotes dysregulated
T859 627-634 Protein denotes SHARPIN
T860 635-644 Negative_regulation denotes deficient
T861 749-756 Protein denotes SHARPIN
T862 758-768 Negative_regulation denotes deficiency
T863 855-873 Protein denotes TOLL-like receptor
T864 875-878 Protein denotes TLR
T865 1053-1057 Protein denotes CD40
T866 1059-1063 Protein denotes CD80
T867 1069-1073 Protein denotes CD86
T868 1014-1024 Gene_expression denotes expression
T869 1014-1024 Gene_expression denotes expression
T870 1014-1024 Gene_expression denotes expression
T871 1001-1010 Positive_regulation denotes increases
T872 1001-1010 Positive_regulation denotes increases
T873 1001-1010 Positive_regulation denotes increases
T874 1095-1102 Protein denotes SHARPIN
T875 1188-1191 Protein denotes IL6
T876 1193-1200 Protein denotes IL12P70
T877 1202-1207 Protein denotes GMCSF
T878 1103-1112 Negative_regulation denotes deficient
T879 1130-1143 Transcription denotes transcription
T880 1130-1143 Transcription denotes transcription
T881 1130-1143 Transcription denotes transcription
T882 1148-1157 Localization denotes secretion
T883 1148-1157 Localization denotes secretion
T884 1148-1157 Localization denotes secretion
T885 1122-1129 Negative_regulation denotes reduced
T886 1122-1129 Negative_regulation denotes reduced
T887 1122-1129 Negative_regulation denotes reduced
T888 1267-1272 Protein denotes NF-κB
T889 1296-1303 Protein denotes MAPK1/3
T890 1305-1309 Protein denotes ERK1
T891 1334-1357 Protein denotes p38 MAP kinase isoforms
T892 1363-1367 Protein denotes TBK1
T893 1253-1263 Positive_regulation denotes activation
T894 1563-1567 Protein denotes CD4+
T895 1600-1607 Protein denotes Sharpin
T896 1592-1596 Negative_regulation denotes loss
T2139 2922-2925 Protein denotes Th1
T2140 2876-2885 Localization denotes secretion
T2141 2866-2875 Negative_regulation denotes decreased
T2142 3034-3038 Protein denotes IL12
T2143 3106-3109 Protein denotes IL5
T2144 3148-3151 Protein denotes IL5
T2145 3152-3161 Negative_regulation denotes deficient
T2146 3341-3348 Protein denotes SHARPIN
T2147 3445-3449 Protein denotes TNFα
T2148 3468-3473 Protein denotes NF-κB
T2149 3493-3500 Protein denotes SHARPIN
T2150 3563-3567 Protein denotes TNFα
T2151 3501-3510 Negative_regulation denotes deficient
T2152 4004-4008 Protein denotes CD4+
T2153 4215-4222 Protein denotes SHARPIN
T2154 4223-4232 Negative_regulation denotes deficient
T2155 4284-4299 Protein denotes SHARPIN protein
T2156 4379-4384 Protein denotes NF-κB
T2157 4276-4280 Negative_regulation denotes lack
T2158 4385-4395 Positive_regulation denotes activation
T2159 4370-4378 Negative_regulation denotes impaired
T2160 4459-4462 Protein denotes Th1
T2161 4472-4482 Gene_expression denotes production
T2162 4449-4458 Positive_regulation denotes stimulate
T2163 4569-4576 Protein denotes SHARPIN
T2164 4668-4675 Protein denotes SHARPIN
T2165 4676-4685 Negative_regulation denotes deficient
T2771 4777-4792 Protein denotes SHARPIN protein
T2772 4936-4943 Protein denotes SHARPIN
T2773 4982-4991 Protein denotes ubiquitin
T2774 5017-5050 Protein denotes zinc-finger Ran-binding protein 2
T2775 5052-5057 Protein denotes ZFRBP
T2776 5059-5065 Entity denotes domain
T2777 5150-5165 Protein denotes SHARPIN protein
T2778 5214-5221 Protein denotes SHARPIN
T2779 5135-5142 Gene_expression denotes present
T2780 5307-5319 Protein denotes Sharpin gene
T2781 5538-5545 Protein denotes Sharpin
T2782 5524-5534 Transcription denotes expression
T2783 5678-5690 Protein denotes Sharpin mRNA
T2784 5771-5780 Negative_regulation denotes decreased
T2785 5838-5850 Protein denotes Sharpin mRNA
T2786 5825-5834 Negative_regulation denotes reduction
T2787 5924-5931 Protein denotes Sharpin
T2788 6025-6040 Protein denotes SHARPIN protein
T2789 6042-6049 Protein denotes Fig. 1E
T2790 5896-5908 Gene_expression denotes Transfection
T2791 5896-5908 Positive_regulation denotes Transfection
T2792 5993-6004 Entity denotes cytoplasmic
T2793 6005-6017 Localization denotes localization
T2794 6005-6017 Localization denotes localization
T4265 10451-10454 Protein denotes IL6
T4266 10456-10463 Protein denotes IL12P70
T4267 10469-10474 Protein denotes GMCSF
T4268 10597-10604 Protein denotes WT BMDC
T4269 10606-10609 Protein denotes Fig
T4270 10438-10447 Localization denotes secretion
T4271 10438-10447 Localization denotes secretion
T4272 10438-10447 Localization denotes secretion
T4273 10532-10540 Gene_expression denotes produced
T4274 10532-10540 Gene_expression denotes produced
T4275 10426-10434 Positive_regulation denotes resulted
T4276 10426-10434 Positive_regulation denotes resulted
T4277 10426-10434 Positive_regulation denotes resulted
T4278 10815-10822 Protein denotes Sharpin
T4279 10823-10832 Negative_regulation denotes deficient
T4280 10870-10877 Protein denotes Sharpin
T4281 11016-11023 Protein denotes IL12P70
T4282 11091-11098 Protein denotes SHARPIN
T4283 11121-11128 Protein denotes IL12P70
T4284 10988-10996 Localization denotes secreted
T4285 11107-11117 Gene_expression denotes production
T4286 11083-11087 Positive_regulation denotes role
T4287 11185-11198 Protein denotes cytokines Il6
T4288 11200-11207 Protein denotes Il12p40
T4289 11158-11164 Transcription denotes levels
T4290 11158-11164 Transcription denotes levels
T4291 11273-11280 Negative_regulation denotes reduced
T4292 11273-11280 Negative_regulation denotes reduced
T5647 13528-13531 Protein denotes MD2
T5648 13493-13503 Gene_expression denotes expression
T5649 13493-13503 Gene_expression denotes expression
T5650 13493-13503 Gene_expression denotes expression
T5651 13633-13647 Protein denotes cytokines IL10
T5652 13665-13669 Protein denotes IL12
T5653 13604-13613 Localization denotes secretion
T5654 13670-13679 Localization denotes secretion
T5655 13657-13664 Negative_regulation denotes inhibit
T5656 13802-13806 Protein denotes IL10
T5657 13858-13862 Protein denotes IL10
T5658 13910-13917 Protein denotes IL12P70
T5659 13812-13822 Positive_regulation denotes stimulated
T5660 13897-13906 Localization denotes secretion
T5661 13871-13882 Positive_regulation denotes responsible
T5662 13887-13896 Negative_regulation denotes decreased
T5663 13980-13983 Protein denotes TLR
T5664 13942-13952 Gene_expression denotes expression
T5665 13932-13941 Positive_regulation denotes Increased
T5666 14082-14085 Protein denotes A20
T5667 14188-14191 Protein denotes A20
T5668 14170-14184 Gene_expression denotes overexpression
T5669 14170-14184 Positive_regulation denotes overexpression
T5670 14159-14165 Positive_regulation denotes ruling
T5671 14159-14165 Positive_regulation denotes ruling
T5672 15035-15039 Protein denotes TLR3
T5673 15147-15152 Protein denotes NF-κB
T5674 15154-15158 Protein denotes TBK1
T5675 15159-15163 Protein denotes IRF3
T5676 15091-15100 Regulation denotes regulated
T5677 15091-15100 Regulation denotes regulated
T5678 15091-15100 Regulation denotes regulated
T5679 15217-15222 Protein denotes NF-κB
T5680 15224-15228 Protein denotes TBK1
T5681 15229-15233 Protein denotes IRF3
T5682 15242-15246 Protein denotes MAPK
T5683 15319-15326 Protein denotes Sharpin
T5684 15327-15336 Negative_regulation denotes deficient
T5685 15383-15393 Protein denotes IκB kinase
T5686 15395-15401 Protein denotes IKK1/2
T5687 15427-15432 Protein denotes NF-κB
T5688 15512-15517 Protein denotes NF-κB
T5689 15528-15532 Protein denotes IκBα
T5690 15548-15575 Protein denotes NF-κB transcription factors
T5691 15360-15375 Phosphorylation denotes phosphorylation
T5692 15360-15375 Phosphorylation denotes phosphorylation
T5693 15453-15468 Phosphorylation denotes phosphorylation
T5694 15453-15468 Phosphorylation denotes phosphorylation
T5695 15493-15504 Protein_catabolism denotes degradation
T5696 15493-15504 Protein_catabolism denotes degradation
T5697 15493-15504 Protein_catabolism denotes degradation
T5698 15585-15592 Entity denotes nucleus
T5699 15343-15359 Positive_regulation denotes Stimulus-induced
T5700 15343-15359 Positive_regulation denotes Stimulus-induced
T5701 15444-15452 Positive_regulation denotes allowing
T5702 15444-15452 Positive_regulation denotes allowing
T5703 15444-15452 Positive_regulation denotes allowing
T5704 15473-15492 Positive_regulation denotes proteasome-mediated
T5705 15473-15492 Positive_regulation denotes proteasome-mediated
T5706 15473-15492 Positive_regulation denotes proteasome-mediated
T5707 15536-15543 Localization denotes release
T5708 15818-15822 Protein denotes TBK1
T5709 15824-15830 Protein denotes ERK1/2
T5710 15836-15839 Protein denotes p38
T5711 15808-15814 Positive_regulation denotes levels
T5712 15808-15814 Positive_regulation denotes levels
T5713 15840-15855 Phosphorylation denotes phosphorylation
T5714 15840-15855 Phosphorylation denotes phosphorylation
T5715 15840-15855 Phosphorylation denotes phosphorylation
T5716 15790-15799 Negative_regulation denotes exhibited
T5717 15790-15799 Negative_regulation denotes exhibited
T5718 15808-15814 Positive_regulation denotes levels
T5719 15808-15814 Positive_regulation denotes levels
T5720 15808-15814 Positive_regulation denotes levels
T5721 15941-15961 Protein denotes SHARPIN decreased NF
T5722 15962-15964 Protein denotes κB
T5723 15995-15999 Protein denotes TBK1
T5724 16000-16004 Protein denotes IRF3
T5725 16006-16010 Protein denotes ERK1
T5726 16018-16021 Protein denotes p38
T5727 15919-15926 Negative_regulation denotes absence
T5728 15965-15975 Positive_regulation denotes activation
T5729 15988-15994 Regulation denotes affect
T5730 15988-15994 Regulation denotes affect
T5731 15988-15994 Regulation denotes affect
T6553 16757-16761 Protein denotes IL12
T6554 16862-16869 Protein denotes SHARPIN
T6555 16762-16772 Gene_expression denotes production
T6556 16747-16756 Negative_regulation denotes defective
T6557 16988-16992 Protein denotes CD4+
T6558 17006-17013 Protein denotes WT BMDC
T6559 17108-17112 Protein denotes IFNγ
T6560 17219-17222 Protein denotes Th1
T6561 17294-17313 Protein denotes TLR2 ligand Pam3CYS
T6562 17362-17365 Protein denotes Th1
T6563 17370-17373 Protein denotes Th2
T6564 17350-17356 Positive_regulation denotes induce
T6565 17350-17356 Positive_regulation denotes induce
T6566 17411-17418 Protein denotes WT BMDC
T6567 17427-17438 Protein denotes robust IFNγ
T6568 17439-17449 Gene_expression denotes production
T6569 17419-17426 Positive_regulation denotes induced
T6570 17520-17523 Protein denotes Th1
T6571 17620-17624 Protein denotes IL12
T6572 17666-17670 Protein denotes TLR2
T6573 17512-17519 Negative_regulation denotes reduced
T6574 17625-17635 Gene_expression denotes production
T6575 17600-17606 Negative_regulation denotes report
T6576 17610-17619 Negative_regulation denotes decreased
T6577 17720-17723 Protein denotes IL4
T6578 17875-17903 Protein denotes Th17-specific cytokine IL17A
T6579 17861-17871 Gene_expression denotes production
T6580 18047-18050 Protein denotes Th1
T6581 18134-18137 Protein denotes IL2
T6582 18216-18223 Protein denotes WT BMDC
T6583 18237-18240 Protein denotes IL2
T6584 18138-18148 Gene_expression denotes production
T6585 18224-18231 Positive_regulation denotes induced
T6586 18121-18130 Positive_regulation denotes effective
T6587 18357-18361 Protein denotes CD4+
T9454 19408-19426 Protein denotes mouse Sharpin gene
T9455 19435-19441 Regulation denotes affect
T9456 19814-19819 Protein denotes NF-κB
T9457 19925-19928 Protein denotes Th2
T9458 20029-20036 Protein denotes SHARPIN
T9459 20750-20754 Protein denotes TNFα
T9460 20837-20840 Protein denotes TNF
T9461 20881-20888 Protein denotes SHARPIN
T9462 20867-20877 Negative_regulation denotes deficiency
T9463 21110-21117 Protein denotes caspase
T9464 21233-21238 Protein denotes DAMPs
T9465 21359-21364 Protein denotes HMGB1
T9466 21208-21215 Localization denotes release
T9467 21365-21375 Binding denotes recognized
T9468 21427-21430 Protein denotes Th1
T9469 21498-21501 Protein denotes Th1
T9470 21618-21622 Protein denotes IL12
T9471 21759-21766 Protein denotes IL12P70
T9472 21767-21777 Gene_expression denotes production
T9473 21751-21758 Negative_regulation denotes reduced
T9474 21805-21812 Protein denotes IL12P70
T9475 21892-21912 Protein denotes IL12P35- and IL12P40
T9476 21791-21801 Positive_regulation denotes importance
T9477 21997-22002 Protein denotes NF-κB
T9478 22004-22007 Protein denotes TBK
T9479 22008-22012 Protein denotes IRF3
T9480 22018-22022 Protein denotes MAPK
T9481 22161-22166 Protein denotes NF-κB
T9482 22288-22292 Protein denotes TBK1
T9483 22293-22297 Protein denotes IRF3
T9484 22299-22303 Protein denotes ERK1
T9485 22310-22313 Protein denotes p38
T9486 22349-22354 Protein denotes NF-κB
T9487 22167-22177 Positive_regulation denotes activation
T9488 22194-22203 Negative_regulation denotes inhibited
T9489 22274-22284 Positive_regulation denotes activation
T9490 22274-22284 Positive_regulation denotes activation
T9491 22274-22284 Positive_regulation denotes activation
T9492 22274-22284 Positive_regulation denotes activation
T9493 22509-22514 Protein denotes NF-κB
T9494 22530-22533 Protein denotes IL1
T9495 22515-22525 Positive_regulation denotes activation
T9496 22534-22547 Transcription denotes transcription
T9497 22489-22498 Positive_regulation denotes increased
T9498 22489-22498 Positive_regulation denotes increased
T9499 22515-22525 Positive_regulation denotes activation
T9500 22730-22737 Protein denotes SHARPIN
T9501 22808-22813 Protein denotes NF-κB
T9502 22855-22860 Protein denotes NF-κB
T9503 22998-23003 Protein denotes NF-κB
T9504 22841-22851 Positive_regulation denotes activation
T9505 23097-23102 Protein denotes NF-κB
T9506 23211-23216 Protein denotes NF-κB
T9507 23103-23113 Positive_regulation denotes activation
T9508 23083-23093 Negative_regulation denotes inhibition
T9509 23316-23321 Protein denotes NF-κB
T9510 23507-23514 Protein denotes Sharpin
T9511 23572-23579 Protein denotes SHARPIN
T9512 23615-23620 Protein denotes NF-κB
T9513 23650-23657 Protein denotes Sharpin
T9514 23621-23631 Positive_regulation denotes activation
T9515 23658-23668 Gene_expression denotes expression
T9516 23621-23631 Positive_regulation denotes activation
T9517 23789-23803 Protein denotes NF-κB subunits
T9518 23864-23869 Protein denotes NF-κB
T9519 24037-24040 Protein denotes p52
T9520 24066-24073 Protein denotes SHARPIN
T9521 24119-24124 Protein denotes NF-κB
T9522 24137-24140 Protein denotes p52
T9523 24141-24145 Protein denotes RELB
T9524 24029-24036 Gene_expression denotes produce
T9525 24074-24083 Negative_regulation denotes deficient
T9526 24261-24268 Protein denotes Sharpin
T9527 24293-24298 Protein denotes NF-κB
T9528 24421-24425 Protein denotes TLR4
T9529 24430-24434 Protein denotes TLR3
T9530 24414-24420 Binding denotes engage
T9531 24484-24488 Protein denotes TLR4
T9532 24561-24566 Protein denotes NF-κB
T9533 24610-24613 Protein denotes TLR
T9534 24454-24464 Gene_expression denotes expression
T9535 24567-24577 Positive_regulation denotes activation
T9536 24614-24624 Gene_expression denotes expression
T9537 24654-24658 Protein denotes TLR4
T9538 24671-24676 Protein denotes MYD88
T9539 24691-24695 Protein denotes TRIF
T9540 24724-24728 Protein denotes TLR3
T9541 24766-24770 Protein denotes TRIF
T9542 24646-24653 Positive_regulation denotes engages
T9543 24811-24816 Protein denotes NF-κB
T9544 24862-24869 Protein denotes Sharpin
T9545 24970-24974 Protein denotes RIP1
T9546 24979-24984 Protein denotes TRAF6
T9547 24817-24827 Positive_regulation denotes activation
T9548 24879-24889 Binding denotes interferes
T9549 24879-24889 Binding denotes interferes
T9550 24879-24889 Binding denotes interferes
T9551 25024-25031 Protein denotes SHARPIN
T9552 25089-25098 Protein denotes ubiquitin
T9553 25099-25104 Protein denotes chain
T9554 25105-25111 Protein denotes ligase
T9555 25124-25129 Protein denotes NF-κB
T9556 25148-25152 Protein denotes RIP1
T9557 25196-25214 Protein denotes TNFα-stimulated NF
T9558 25215-25217 Protein denotes κB
T9559 25032-25041 Binding denotes interacts
T9560 25218-25228 Positive_regulation denotes activation
T9561 25218-25228 Positive_regulation denotes activation
T9562 25234-25241 Protein denotes Sharpin
T9563 25310-25314 Protein denotes TNFα
T9564 25323-25328 Protein denotes NF-κB
T9565 25354-25358 Protein denotes TNFR
T9566 25363-25366 Protein denotes TLR
T9567 25478-25485 Protein denotes SHARPIN
T9568 25515-25520 Protein denotes NF-κB
T9569 25521-25531 Positive_regulation denotes activation
T9570 25449-25459 Regulation denotes regulation
T9571 25599-25606 Protein denotes SHARPIN
T9572 25671-25676 Protein denotes NF-κB
T9573 25614-25624 Gene_expression denotes production
T9574 25701-25704 Protein denotes Th1
T9575 25728-25735 Protein denotes Sharpin
T9576 25736-25745 Negative_regulation denotes deficient
T9937 26642-26644 Protein denotes WT
T9938 26835-26842 Protein denotes Sharpin
T10323 27233-27240 Protein denotes Sharpin
T10324 27356-27367 Protein denotes CCG CCC GCC
T10325 27368-27375 Protein denotes GGC GGT
T10326 27390-27401 Protein denotes AAG CTT CTA
T10327 27402-27427 Protein denotes GGT GGA AGC TGC AGC AAG A
T10328 27433-27445 Protein denotes Sharpin cDNA
T10329 27554-27581 Protein denotes recombinant SHARPIN protein
T10330 27546-27553 Gene_expression denotes express
T10331 27891-27895 Protein denotes FLAG
T10332 27896-27903 Protein denotes SHARPIN
T10333 27904-27914 Gene_expression denotes expression
T10334 27904-27914 Gene_expression denotes expression
T10925 28588-28593 Protein denotes CD11c
T10926 28595-28603 Protein denotes MCD11C20
T10927 28743-28750 Protein denotes PE-anti
T10928 28751-28755 Protein denotes CD14
T10929 28929-28938 Protein denotes avidin-PE
T10930 28976-28987 Protein denotes collagenase
T10931 29116-29121 Protein denotes CD11c
T10932 29123-29126 Protein denotes HL3
T10933 29177-29186 Protein denotes FITC-anti
T10934 29187-29191 Protein denotes CD8α
T11296 29716-29720 Protein denotes CD4+
T11297 29939-29943 Protein denotes IFNγ
T11298 29945-29948 Protein denotes IL4
T11299 29950-29953 Protein denotes IL2
T11300 29959-29964 Protein denotes IL17A
T11301 29926-29935 Localization denotes secretion
T11302 29926-29935 Localization denotes secretion
T11303 29926-29935 Localization denotes secretion
T11304 29926-29935 Localization denotes secretion
T11906 30419-30422 Protein denotes TRI
T11907 30503-30506 Protein denotes Il6
T11908 30508-30515 Protein denotes Il12p40
T11909 30528-30540 Protein denotes Sharpin mRNA
T11910 30489-30499 Gene_expression denotes expression
T11911 30489-30499 Gene_expression denotes expression
T11912 30489-30499 Gene_expression denotes expression
T11913 30821-30840 Protein denotes RNasin Ribonuclease
T11914 30856-30883 Protein denotes M-MLV Reverse Transcriptase
T11915 31218-31222 Protein denotes Actb
T11916 31224-31227 Protein denotes IL6
T11917 31229-31236 Protein denotes IL12p40
T11918 31238-31242 Protein denotes Ifnβ
T11919 31255-31262 Protein denotes Sharpin
T11920 31370-31377 Protein denotes β-actin
T12079 31599-31624 Protein denotes RPMI-1640 complete medium
T12672 32791-32797 Protein denotes p-IκBα
T12673 32807-32811 Protein denotes IκBα
T12674 32823-32827 Protein denotes TBK1
T12675 32838-32841 Protein denotes p38
T12676 32851-32854 Protein denotes p38
T12677 32902-32906 Protein denotes Beta
T12678 32907-32912 Protein denotes actin
T13246 6129-6136 Protein denotes SHARPIN
T13247 6233-6236 Protein denotes UBL
T13248 6238-6247 Protein denotes ubiquitin
T13249 6265-6270 Protein denotes ZFRBP
T13250 6272-6305 Protein denotes zinc-finger Ran-Binding protein 2
T13251 6355-6370 Protein denotes SHARPIN protein
T13252 6730-6737 Protein denotes Sharpin
T13253 6826-6833 Protein denotes WT BMDC
T13254 6797-6807 Transcription denotes expression
T13255 7178-7182 Protein denotes FLAG
T13256 7183-7190 Protein denotes SHARPIN
T14953 14291-14295 Protein denotes TLR4
T14954 14300-14303 Protein denotes MD2
T14955 14329-14333 Protein denotes IL10
T14956 14304-14314 Gene_expression denotes expression
T14957 14304-14314 Gene_expression denotes expression
T14958 14319-14328 Negative_regulation denotes decreased
T14959 14319-14328 Negative_regulation denotes decreased
T14960 14334-14343 Localization denotes secretion
T14961 14319-14328 Negative_regulation denotes decreased
T14962 14453-14457 Protein denotes TLR4
T14963 14458-14461 Protein denotes MD2
T14964 14470-14474 Protein denotes CD14
T14965 14764-14768 Protein denotes IL10
T14966 14969-14972 Protein denotes qRT
T14967 15006-15009 Protein denotes A20
T14968 14992-15002 Gene_expression denotes production
T15733 18563-18567 Protein denotes CD4+
T15734 18634-18653 Protein denotes MHC haplotype: H-2b
T15735 18832-18836 Protein denotes CD4+
T15736 18887-18900 Protein denotes MHC haplotype
T15737 18972-18976 Protein denotes IFNγ
T15738 18978-18981 Protein denotes IL4
T15739 18987-18990 Protein denotes IL2
T15740 18959-18968 Localization denotes secretion
T15741 18959-18968 Localization denotes secretion
T15742 18959-18968 Localization denotes secretion
T5633 12924-12929 Protein denotes NF-κB
T5634 13221-13224 Protein denotes TLR
T5635 13184-13194 Gene_expression denotes expression
T5636 13174-13183 Positive_regulation denotes increased
T5637 13329-13341 Protein denotes LPS receptor
T5638 13365-13369 Protein denotes TLR4
T5639 13394-13398 Protein denotes CD14
T5640 13403-13435 Protein denotes myeloid differentiation factor 2
T5641 13437-13440 Protein denotes MD2
T5642 13441-13445 Protein denotes LY96
T5643 13311-13321 Gene_expression denotes expression
T5644 13311-13321 Gene_expression denotes expression
T5645 13514-13518 Protein denotes CD14
T5646 13523-13527 Protein denotes TLR4
T14226 11670-11677 Protein denotes IL12P70
T14227 11679-11682 Protein denotes IL6
T14228 11688-11693 Protein denotes GMCSF
T14229 11900-11907 Protein denotes IL12P70
T14230 11912-11915 Protein denotes IL6
T14231 11981-12003 Protein denotes Sharpin-containing BAC
T14232 12240-12247 Protein denotes IL12P70
T14233 12248-12258 Gene_expression denotes production
T14538 12662-12665 Protein denotes qRT
T15303 16099-16104 Protein denotes NF-κB
T15304 16085-16095 Negative_regulation denotes Inhibition
T15305 16386-16391 Protein denotes NF-κB
T15306 16393-16397 Protein denotes TBK1
T15307 16398-16402 Protein denotes IRF3
T15308 16404-16408 Protein denotes ERK1
T15309 16416-16419 Protein denotes p38
T15310 16440-16444 Protein denotes Beta
T15311 16445-16450 Protein denotes actin
T15312 16516-16522 Protein denotes p-IκBα
T15313 16581-16587 Protein denotes ImageJ
T15314 16589-16592 Protein denotes NIH
T15315 16493-16499 Gene_expression denotes levels
T2135 1771-1778 Protein denotes SHARPIN
T2136 1908-1917 Gene_expression denotes expressed
T2137 1997-2009 Protein denotes Sharpin gene
T2138 2889-2897 Protein denotes IFNγ [5]
R7796 T9454 T9455 themeOf mouse Sharpin gene,affect
R7798 T9543 T9547 themeOf NF-κB,activation
R7799 T9544 T9548 themeOf Sharpin,interferes
R7800 T9544 T9549 themeOf Sharpin,interferes
R7801 T9544 T9550 themeOf Sharpin,interferes
R7802 T9545 T9549 themeOf RIP1,interferes
R7803 T9546 T9550 themeOf TRAF6,interferes
R7804 T9551 T9559 themeOf SHARPIN,interacts
R7805 T9461 T9462 themeOf SHARPIN,deficiency
R7806 T9464 T9466 themeOf DAMPs,release
R7807 T9465 T9467 themeOf HMGB1,recognized
R7808 T9557 T9560 themeOf TNFα-stimulated NF,activation
R7809 T9471 T9472 themeOf IL12P70,production
R7810 T9472 T9473 themeOf production,reduced
R7811 T9474 T9476 themeOf IL12P70,importance
R7812 T9558 T9561 themeOf κB,activation
R7813 T9568 T9569 themeOf NF-κB,activation
R7814 T9481 T9487 themeOf NF-κB,activation
R7815 T9482 T9489 themeOf TBK1,activation
R7816 T9483 T9490 themeOf IRF3,activation
R7817 T9484 T9491 themeOf ERK1,activation
R7818 T9485 T9492 themeOf p38,activation
R7819 T9487 T9488 themeOf activation,inhibited
R7820 T9493 T9495 themeOf NF-κB,activation
R7821 T9494 T9496 themeOf IL1,transcription
R630 T840 T847 themeOf Sharpin,mutations
R631 T841 T851 themeOf SHANK,associated
R632 T842 T848 themeOf RH,mutations
R634 T843 T849 themeOf Rbckl1,mutations
R636 T844 T850 themeOf Sipl1,mutations
R1787 T2161 T2162 themeOf production,stimulate
R1788 T2164 T2165 themeOf SHARPIN,deficient
R2284 T2772 T2776 partOf SHARPIN,domain
R2285 T2774 T2776 partOf zinc-finger Ran-binding protein 2,domain
R2286 T2775 T2776 partOf ZFRBP,domain
R2287 T2777 T2779 themeOf SHARPIN protein,present
R2288 T2781 T2782 themeOf Sharpin,expression
R2289 T2783 T2784 themeOf Sharpin mRNA,decreased
R2290 T2785 T2786 themeOf Sharpin mRNA,reduction
R2291 T2787 T2790 themeOf Sharpin,Transfection
R2292 T2788 T2793 themeOf SHARPIN protein,localization
R2293 T2789 T2794 themeOf Fig. 1E,localization
R2294 T2790 T2791 themeOf Transfection,Transfection
R2295 T2792 T2793 locationOf cytoplasmic,localization
R2296 T2792 T2794 locationOf cytoplasmic,localization
R3530 T4265 T4270 themeOf IL6,secretion
R3531 T4266 T4271 themeOf IL12P70,secretion
R3532 T4267 T4272 themeOf GMCSF,secretion
R3533 T4268 T4273 themeOf WT BMDC,produced
R3534 T4269 T4274 themeOf Fig,produced
R3535 T4270 T4275 themeOf secretion,resulted
R3536 T4271 T4276 themeOf secretion,resulted
R3537 T4272 T4277 themeOf secretion,resulted
R3538 T4278 T4279 themeOf Sharpin,deficient
R3539 T4281 T4284 themeOf IL12P70,secreted
R3540 T4282 T4286 causeOf SHARPIN,role
R3541 T4283 T4285 themeOf IL12P70,production
R3542 T4285 T4286 themeOf production,role
R3543 T4287 T4289 themeOf cytokines Il6,levels
R3544 T4288 T4290 themeOf Il12p40,levels
R3545 T4289 T4291 themeOf levels,reduced
R3546 T4290 T4292 themeOf levels,reduced
R4543 T5635 T5636 themeOf expression,increased
R4544 T5637 T5643 themeOf LPS receptor,expression
R4545 T5638 T5644 themeOf TLR4,expression
R4546 T5645 T5648 themeOf CD14,expression
R4547 T5646 T5649 themeOf TLR4,expression
R4548 T5647 T5650 themeOf MD2,expression
R4549 T5651 T5653 themeOf cytokines IL10,secretion
R4550 T5651 T5655 causeOf cytokines IL10,inhibit
R4551 T5652 T5654 themeOf IL12,secretion
R4552 T5654 T5655 themeOf secretion,inhibit
R4553 T5656 T5659 themeOf IL10,stimulated
R4554 T5657 T5661 causeOf IL10,responsible
R4555 T5658 T5660 themeOf IL12P70,secretion
R4556 T5660 T5661 themeOf secretion,responsible
R4557 T5660 T5662 themeOf secretion,decreased
R4558 T5663 T5664 themeOf TLR,expression
R4559 T5664 T5665 themeOf expression,Increased
R4560 T5667 T5668 themeOf A20,overexpression
R4561 T5668 T5669 themeOf overexpression,overexpression
R4562 T5668 T5670 themeOf overexpression,ruling
R4563 T5669 T5671 themeOf overexpression,ruling
R4564 T5673 T5676 themeOf NF-κB,regulated
R4565 T5674 T5677 themeOf TBK1,regulated
R4566 T5675 T5678 themeOf IRF3,regulated
R4567 T5683 T5684 themeOf Sharpin,deficient
R4568 T5685 T5691 themeOf IκB kinase,phosphorylation
R4569 T5686 T5692 themeOf IKK1/2,phosphorylation
R4570 T5686 T5695 themeOf IKK1/2,degradation
R4571 T5688 T5693 themeOf NF-κB,phosphorylation
R4572 T5688 T5696 themeOf NF-κB,degradation
R4573 T5689 T5694 themeOf IκBα,phosphorylation
R4574 T5689 T5697 themeOf IκBα,degradation
R4575 T5690 T5707 themeOf NF-κB transcription factors,release
R4576 T5691 T5699 themeOf phosphorylation,Stimulus-induced
R4577 T5692 T5700 themeOf phosphorylation,Stimulus-induced
R4578 T5695 T5701 themeOf degradation,allowing
R4579 T5695 T5704 themeOf degradation,proteasome-mediated
R4580 T5696 T5702 themeOf degradation,allowing
R4581 T5696 T5705 themeOf degradation,proteasome-mediated
R4582 T5697 T5703 themeOf degradation,allowing
R4583 T5697 T5706 themeOf degradation,proteasome-mediated
R4584 T5698 T5707 locationOf nucleus,release
R4585 T5708 T5711 themeOf TBK1,levels
R4586 T5708 T5713 themeOf TBK1,phosphorylation
R4587 T5709 T5712 themeOf ERK1/2,levels
R4588 T5709 T5714 themeOf ERK1/2,phosphorylation
R4589 T5710 T5715 themeOf p38,phosphorylation
R4590 T5711 T5716 themeOf levels,exhibited
R4591 T5712 T5717 themeOf levels,exhibited
R4592 T5713 T5718 themeOf phosphorylation,levels
R4593 T5714 T5719 themeOf phosphorylation,levels
R4594 T5715 T5720 themeOf phosphorylation,levels
R4595 T5721 T5727 themeOf SHARPIN decreased NF,absence
R4596 T5722 T5728 themeOf κB,activation
R4597 T5723 T5729 themeOf TBK1,affect
R4598 T5724 T5730 themeOf IRF3,affect
R4599 T5725 T5731 themeOf ERK1,affect
R4600 T5727 T5729 causeOf absence,affect
R4601 T5727 T5730 causeOf absence,affect
R4602 T5727 T5731 causeOf absence,affect
R5313 T6553 T6555 themeOf IL12,production
R5314 T6555 T6556 themeOf production,defective
R5315 T6562 T6564 themeOf Th1,induce
R5316 T6563 T6565 themeOf Th2,induce
R5317 T6566 T6569 causeOf WT BMDC,induced
R5318 T6567 T6568 themeOf robust IFNγ,production
R5319 T6568 T6569 themeOf production,induced
R5320 T6570 T6573 themeOf Th1,reduced
R5321 T6571 T6574 themeOf IL12,production
R5322 T6574 T6575 themeOf production,report
R5323 T6574 T6576 themeOf production,decreased
R5324 T6578 T6579 themeOf Th17-specific cytokine IL17A,production
R5325 T6581 T6584 themeOf IL2,production
R5326 T6582 T6585 causeOf WT BMDC,induced
R5327 T6583 T6585 themeOf IL2,induced
R5328 T6584 T6586 themeOf production,effective
R7822 T9569 T9570 themeOf activation,regulation
R7823 T9495 T9497 themeOf activation,increased
R7824 T9496 T9498 themeOf transcription,increased
R7825 T9496 T9499 themeOf transcription,activation
R7826 T9571 T9573 themeOf SHARPIN,production
R7827 T9575 T9576 themeOf Sharpin,deficient
R7828 T9502 T9504 themeOf NF-κB,activation
R7829 T9505 T9507 themeOf NF-κB,activation
R7830 T9507 T9508 themeOf activation,inhibition
R7831 T9512 T9514 themeOf NF-κB,activation
R7832 T9513 T9515 themeOf Sharpin,expression
R7833 T9515 T9516 themeOf expression,activation
R7834 T9519 T9524 themeOf p52,produce
R7835 T9520 T9525 themeOf SHARPIN,deficient
R7836 T9529 T9530 themeOf TLR3,engage
R7837 T9531 T9534 themeOf TLR4,expression
R7838 T9532 T9535 themeOf NF-κB,activation
R7839 T9533 T9536 themeOf TLR,expression
R7840 T9537 T9542 themeOf TLR4,engages
R8507 T10329 T10330 themeOf recombinant SHARPIN protein,express
R8508 T10331 T10333 themeOf FLAG,expression
R8509 T10332 T10334 themeOf SHARPIN,expression
R9380 T11297 T11301 themeOf IFNγ,secretion
R9381 T11298 T11302 themeOf IL4,secretion
R9382 T11299 T11303 themeOf IL2,secretion
R9383 T11300 T11304 themeOf IL17A,secretion
R9881 T11907 T11910 themeOf Il6,expression
R9882 T11908 T11911 themeOf Il12p40,expression
R9883 T11909 T11912 themeOf Sharpin mRNA,expression
R11003 T13253 T13254 themeOf WT BMDC,expression
R11889 T14232 T14233 themeOf IL12P70,production
R12530 T14953 T14956 themeOf TLR4,expression
R12531 T14954 T14957 themeOf MD2,expression
R12532 T14955 T14960 themeOf IL10,secretion
R12533 T14956 T14958 themeOf expression,decreased
R12534 T14957 T14959 themeOf expression,decreased
R12535 T14960 T14961 themeOf secretion,decreased
R12536 T14967 T14968 themeOf A20,production
R12792 T15303 T15304 themeOf NF-κB,Inhibition
R12793 T15312 T15315 themeOf p-IκBα,levels
R13166 T15737 T15740 themeOf IFNγ,secretion
R13167 T15738 T15741 themeOf IL4,secretion
R13168 T15739 T15742 themeOf IL2,secretion
R4542 T5634 T5635 themeOf TLR,expression
R633 T842 T852 themeOf RH,associated
R635 T843 T853 themeOf Rbckl1,associated
R637 T844 T854 themeOf Sipl1,associated
R638 T845 T855 themeOf T helper1,secretion
R639 T846 T856 themeOf Th1,secretion
R640 T855 T857 themeOf secretion,dysregulated
R641 T856 T858 themeOf secretion,dysregulated
R642 T859 T860 themeOf SHARPIN,deficient
R643 T861 T862 themeOf SHARPIN,deficiency
R644 T865 T868 themeOf CD40,expression
R645 T866 T869 themeOf CD80,expression
R646 T867 T870 themeOf CD86,expression
R647 T868 T871 themeOf expression,increases
R648 T869 T872 themeOf expression,increases
R649 T870 T873 themeOf expression,increases
R650 T874 T878 themeOf SHARPIN,deficient
R651 T875 T879 themeOf IL6,transcription
R652 T875 T882 themeOf IL6,secretion
R653 T876 T880 themeOf IL12P70,transcription
R654 T876 T883 themeOf IL12P70,secretion
R655 T877 T881 themeOf GMCSF,transcription
R656 T877 T884 themeOf GMCSF,secretion
R657 T879 T885 themeOf transcription,reduced
R658 T880 T886 themeOf transcription,reduced
R659 T881 T887 themeOf transcription,reduced
R660 T888 T893 themeOf NF-κB,activation
R661 T895 T896 themeOf Sharpin,loss
R1773 T2135 T2136 themeOf SHARPIN,expressed
R1776 T2138 T2140 themeOf IFNγ [5],secretion
R1778 T2140 T2141 themeOf secretion,decreased
R1779 T2144 T2145 themeOf IL5,deficient
R1780 T2149 T2151 themeOf SHARPIN,deficient
R1781 T2153 T2154 themeOf SHARPIN,deficient
R1782 T2155 T2157 themeOf SHARPIN protein,lack
R1783 T2156 T2158 themeOf NF-κB,activation
R1784 T2157 T2159 causeOf lack,impaired
R1785 T2158 T2159 themeOf activation,impaired
R1786 T2160 T2161 themeOf Th1,production

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue Negation Speculation
T93 1148-1157 Gene_expression denotes secretion
T94 1188-1191 Protein denotes IL6
T95 1202-1207 Protein denotes GMCSF
T96 1296-1301 Protein denotes MAPK1
T97 1302-1303 Protein denotes 3
T98 1305-1309 Protein denotes ERK1
T99 1310-1311 Protein denotes 2
T100 1317-1323 Protein denotes MAPK11
T101 1324-1326 Protein denotes 12
T102 1327-1329 Protein denotes 13
T103 1330-1332 Protein denotes 14
T104 1363-1367 Protein denotes TBK1
T105 1592-1596 Negative_regulation denotes loss
T106 1600-1607 Protein denotes Sharpin
T972 4223-4232 Negative_regulation denotes deficient
T973 4276-4280 Negative_regulation denotes lack
T974 4284-4291 Protein denotes SHARPIN
T975 4569-4576 Protein denotes SHARPIN
T976 4668-4675 Protein denotes SHARPIN
T977 4676-4685 Negative_regulation denotes deficient
T2214 5993-6004 Entity denotes cytoplasmic
T2215 6005-6017 Localization denotes localization
T2216 6025-6032 Protein denotes SHARPIN
T3241 7409-7417 Negative_regulation denotes mutation
T3242 10056-10064 Positive_regulation denotes resulted
T3243 10068-10077 Positive_regulation denotes increased
T3244 10078-10088 Gene_expression denotes expression
T3245 10078-10088 Gene_expression denotes expression
T3246 10078-10088 Gene_expression denotes expression
T3247 10144-10154 Regulation denotes difference true
T3248 10162-10172 Gene_expression denotes expression
T3249 10144-10154 Regulation denotes difference true
T3250 10162-10172 Gene_expression denotes expression
T3251 10144-10154 Regulation denotes difference true
T3252 7835-7845 Negative_regulation denotes deficiency
T3253 10162-10172 Gene_expression denotes expression
T3254 10247-10257 Negative_regulation denotes deficiency
T3255 8170-8178 Negative_regulation denotes mutation
T3256 9861-9867 Binding denotes ligand
T3257 9890-9896 Binding denotes ligand
T3258 10056-10064 Positive_regulation denotes resulted
T3259 10068-10077 Positive_regulation denotes increased
T3260 10056-10064 Positive_regulation denotes resulted
T3261 10068-10077 Positive_regulation denotes increased
T3262 7401-7408 Protein denotes Sharpin
T3263 9763-9768 Protein denotes CD11c
T3264 9830-9834 Protein denotes CD40
T3265 9836-9840 Protein denotes CD80
T3266 9846-9850 Protein denotes CD86
T3267 9856-9860 Protein denotes TLR3
T3268 9885-9889 Protein denotes TLR4
T3269 10092-10096 Protein denotes CD40
T3270 10098-10102 Protein denotes CD80
T3271 10108-10112 Protein denotes CD86
T3272 10239-10246 Protein denotes SHARPIN
T3273 7536-7541 Protein denotes CD11c
T3274 7542-7546 Protein denotes CD8α
T3275 7552-7557 Protein denotes CD11c
T3276 7596-7601 Protein denotes CD11c
T3277 7602-7608 Protein denotes PDCA-1
T3278 7827-7834 Protein denotes SHARPIN
T3279 8162-8169 Protein denotes Sharpin
T3280 7558-7562 Protein denotes CD8α
T4410 13365-13369 Protein denotes TLR4
T4411 13394-13398 Protein denotes CD14
T4412 13403-13435 Protein denotes myeloid differentiation factor 2
T4413 13437-13440 Protein denotes MD2
T4414 13441-13445 Protein denotes LY96
T4415 13493-13503 Gene_expression denotes expression
T4416 13493-13503 Gene_expression denotes expression
T4417 13493-13503 Gene_expression denotes expression
T4418 13514-13518 Protein denotes CD14
T4419 13523-13527 Protein denotes TLR4
T4420 13528-13531 Protein denotes MD2
T4421 13604-13613 Gene_expression denotes secretion true
T4422 13643-13647 Protein denotes IL10
T4423 13786-13791 Positive_regulation denotes lower true
T4424 13792-13798 Gene_expression denotes levels
T4425 13802-13806 Protein denotes IL10
T4426 13858-13862 Protein denotes IL10
T4427 13932-13941 Positive_regulation denotes Increased
T4428 13942-13952 Gene_expression denotes expression
T4429 14002-14005 Protein denotes A20
T4430 14062-14078 Transcription denotes transcript level
T4431 14082-14085 Protein denotes A20
T4432 14090-14095 Positive_regulation denotes lower true
T4433 14170-14184 Positive_regulation denotes overexpression
T4434 14188-14191 Protein denotes A20
T4435 15035-15039 Protein denotes TLR3
T4436 15040-15041 Protein denotes 4
T4437 15154-15158 Protein denotes TBK1
T4438 15159-15163 Protein denotes IRF3
T4439 15224-15228 Protein denotes TBK1
T4440 15229-15233 Protein denotes IRF3
T4441 15319-15326 Protein denotes Sharpin
T4442 15327-15336 Negative_regulation denotes deficient
T4443 15395-15399 Protein denotes IKK1
T4444 15400-15401 Protein denotes 2
T4445 15444-15452 Positive_regulation denotes allowing
T4446 15444-15452 Positive_regulation denotes allowing
T4447 15453-15468 Phosphorylation denotes phosphorylation
T4448 15484-15492 Positive_regulation denotes mediated
T4449 15493-15504 Protein_catabolism denotes degradation
T4450 15528-15532 Protein denotes IκBα
T4451 15608-15622 Phosphorylation denotes phosphorylated
T4452 15608-15622 Phosphorylation denotes phosphorylated
T4453 15608-15622 Phosphorylation denotes phosphorylated
T4454 15623-15627 Protein denotes IKK1
T4455 15628-15629 Protein denotes 2
T4456 15633-15637 Protein denotes IKK1
T4457 15638-15639 Protein denotes 2
T4458 15645-15649 Protein denotes IκBα
T4459 15653-15657 Protein denotes IκBα
T4460 15714-15723 Negative_regulation denotes decreased
T4461 15714-15723 Negative_regulation denotes decreased
T4462 15714-15723 Negative_regulation denotes decreased
T4463 15818-15822 Protein denotes TBK1
T4464 15824-15828 Protein denotes ERK1
T4465 15829-15830 Protein denotes 2
T5809 17666-17670 Protein denotes TLR2
T5810 17720-17723 Protein denotes IL4
T5811 17737-17745 Gene_expression denotes produced
T5812 17861-17871 Gene_expression denotes production
T5813 17898-17903 Protein denotes IL17A
T5814 17904-17913 Positive_regulation denotes following
T5815 18134-18137 Protein denotes IL2
T5816 18138-18148 Gene_expression denotes production
T5817 18224-18231 Positive_regulation denotes induced
T5818 18232-18236 Positive_regulation denotes more
T5819 18237-18240 Protein denotes IL2
T6684 19414-19421 Protein denotes Sharpin
T6685 19546-19550 Negative_regulation denotes loss
T6686 19554-19561 Protein denotes SHARPIN
T6687 20029-20036 Protein denotes SHARPIN
T6688 20079-20089 Negative_regulation denotes disruption
T6689 20750-20754 Protein denotes TNFα
T6690 20825-20833 Negative_regulation denotes deletion
T6691 20837-20840 Protein denotes TNF
T6692 20867-20877 Negative_regulation denotes deficiency
T6693 20881-20888 Protein denotes SHARPIN
T6694 21359-21364 Protein denotes HMGB1
T6695 21365-21375 Binding denotes recognized
T6696 21379-21383 Protein denotes TLR2
T6697 21384-21385 Protein denotes 4
T6698 21892-21899 Protein denotes IL12P35
T6699 21905-21912 Protein denotes IL12P40
T6700 21913-21922 Negative_regulation denotes deficient
T6701 21913-21922 Negative_regulation denotes deficient
T6702 22008-22012 Protein denotes IRF3
T6703 22274-22284 Positive_regulation denotes activation
T6704 22274-22284 Positive_regulation denotes activation
T6705 22274-22284 Positive_regulation denotes activation
T6706 22274-22284 Positive_regulation denotes activation
T6707 22288-22292 Protein denotes TBK1
T6708 22293-22297 Protein denotes IRF3
T6709 22299-22303 Protein denotes ERK1
T6710 22304-22305 Protein denotes 2
T6711 22314-22321 Negative_regulation denotes was not true
T6712 22314-22321 Negative_regulation denotes was not true
T6713 22314-22321 Negative_regulation denotes was not true
T6714 22314-22321 Negative_regulation denotes was not true
T6715 22730-22737 Protein denotes SHARPIN
T6716 22892-22895 Protein denotes A20
T6717 22900-22904 Protein denotes ITCH
T6718 23495-23503 Negative_regulation denotes deletion
T6719 23507-23514 Protein denotes Sharpin
T6720 23572-23579 Protein denotes SHARPIN
T6721 23639-23646 Negative_regulation denotes absence
T6722 23650-23657 Protein denotes Sharpin
T6723 23658-23668 Gene_expression denotes expression
T6724 24010-24014 Protein denotes p100
T6725 24037-24040 Protein denotes p52
T6726 24066-24073 Protein denotes SHARPIN
T6727 24074-24083 Negative_regulation denotes deficient
T6728 24137-24140 Protein denotes p52
T6729 24141-24145 Protein denotes RELB
T6730 24261-24268 Protein denotes Sharpin
T6731 24269-24277 Negative_regulation denotes mutation
T6732 24414-24420 Binding denotes engage
T6733 24414-24420 Binding denotes engage
T6734 24421-24425 Protein denotes TLR4
T6735 24430-24434 Protein denotes TLR3
T6736 24646-24653 Binding denotes engages
T6737 24654-24658 Protein denotes TLR4
T6738 24671-24676 Protein denotes MYD88
T6739 24691-24695 Protein denotes TRIF
T6740 24724-24728 Protein denotes TLR3
T6741 24729-24739 Positive_regulation denotes stimulated
T6742 24766-24770 Protein denotes TRIF
T6743 24862-24869 Protein denotes Sharpin
T6744 24870-24878 Negative_regulation denotes mutation
T6745 24970-24974 Protein denotes RIP1
T6746 24979-24984 Protein denotes TRAF6
T6747 25024-25031 Protein denotes SHARPIN
T6748 25032-25041 Binding denotes interacts
T6749 25047-25051 Protein denotes HOIP
T6750 25071-25077 Positive_regulation denotes exerts
T6751 25071-25077 Positive_regulation denotes exerts
T6752 25082-25120 Ubiquitination denotes linear-ubiquitin-chain-ligase activity
T6753 25082-25120 Ubiquitination denotes linear-ubiquitin-chain-ligase activity
T6754 25148-25152 Protein denotes RIP1
T6755 25157-25161 Protein denotes NEMO
T6756 25196-25200 Protein denotes TNFα
T6757 25234-25241 Protein denotes Sharpin
T6758 25242-25255 Negative_regulation denotes null mutation
T6759 25256-25264 Negative_regulation denotes disrupts
T6760 25256-25264 Negative_regulation denotes disrupts
T9953 27233-27240 Protein denotes Sharpin
T9954 27433-27440 Protein denotes Sharpin
T9955 27546-27553 Gene_expression denotes express
T9956 27566-27573 Protein denotes SHARPIN
T9957 27705-27712 Protein denotes SHARPIN
T9958 27896-27903 Protein denotes SHARPIN
T9959 27904-27914 Gene_expression denotes expression true
T11350 31370-31377 Protein denotes β-actin
T12755 6129-6136 Protein denotes SHARPIN
T12756 6355-6362 Protein denotes SHARPIN
T12757 6716-6726 Transcription denotes mRNA level true
T12758 6730-6737 Protein denotes Sharpin
T12759 7007-7014 Protein denotes SHARPIN
T12760 7183-7190 Protein denotes SHARPIN
T12761 7207-7216 Entity denotes cytoplasm
T12762 7217-7226 Localization denotes localized
T14990 16505-16509 Protein denotes IKK1
T14991 16510-16511 Protein denotes 2
T14992 16518-16522 Protein denotes IκBα
T11335 30489-30499 Transcription denotes expression true
T11336 30489-30499 Transcription denotes expression true
T11337 30489-30499 Transcription denotes expression true
T11338 30489-30499 Transcription denotes expression true
T11339 30503-30506 Protein denotes Il6
T11340 30508-30515 Protein denotes Il12p40
T11341 30517-30522 Protein denotes Gmcsf
T11342 30528-30535 Protein denotes Sharpin
T11343 30856-30883 Protein denotes M-MLV Reverse Transcriptase
T11344 31218-31222 Protein denotes Actb
T11345 31224-31227 Protein denotes IL6
T11346 31229-31236 Protein denotes IL12p40
T11347 31238-31242 Protein denotes Ifnβ
T11348 31244-31249 Protein denotes Gmcsf
T11349 31255-31262 Protein denotes Sharpin
T14248 12685-12695 Transcription denotes expression true
T14249 12685-12695 Transcription denotes expression true
T14250 12685-12695 Transcription denotes expression true
T14251 12685-12695 Transcription denotes expression true
T14252 12699-12706 Protein denotes Il12p40
T14253 12714-12717 Protein denotes Il6
T14254 12725-12730 Protein denotes Gmcsf
T14255 12742-12746 Protein denotes Ifnb
T950 1771-1778 Protein denotes SHARPIN
T951 1908-1917 Gene_expression denotes expressed
T952 1970-1989 Negative_regulation denotes recessive mutations
T953 1997-2004 Protein denotes Sharpin
T954 2152-2161 Positive_regulation denotes resulting
T955 2152-2161 Positive_regulation denotes resulting
T956 2175-2186 Negative_regulation denotes termination
T957 2195-2204 Transcription denotes synthesis
T958 2209-2216 Negative_regulation denotes absence
T959 2866-2875 Negative_regulation denotes decreased
T960 2876-2885 Gene_expression denotes secretion
T961 2889-2893 Protein denotes IFNγ
T962 3088-3102 Negative_regulation denotes Neutralization
T963 3106-3109 Protein denotes IL5
T964 3148-3151 Protein denotes IL5
T965 3152-3161 Negative_regulation denotes deficient
T966 3341-3348 Protein denotes SHARPIN
T967 3445-3449 Protein denotes TNFα
T968 3493-3500 Protein denotes SHARPIN
T969 3501-3510 Negative_regulation denotes deficient
T970 3563-3567 Protein denotes TNFα
T971 4215-4222 Protein denotes SHARPIN
T5789 16851-16858 Negative_regulation denotes absence
T5790 16862-16869 Protein denotes SHARPIN
T5791 17046-17054 Positive_regulation denotes elicited
T5792 17062-17066 Protein denotes IFNγ
T5793 17067-17077 Gene_expression denotes production
T5794 17091-17104 Gene_expression denotes concentration
T5795 17108-17112 Protein denotes IFNγ
T5796 17160-17165 Positive_regulation denotes lower true
T5797 17273-17277 Protein denotes TLR3
T5798 17278-17279 Protein denotes 4
T5799 17280-17288 Positive_regulation denotes agonists
T5800 17280-17288 Positive_regulation denotes agonists
T5801 17294-17298 Protein denotes TLR2
T5802 17299-17305 Binding denotes ligand
T5803 17306-17313 Protein denotes Pam3CYS
T5804 17395-17402 Protein denotes Pam3CYS
T5805 17419-17426 Positive_regulation denotes induced
T5806 17434-17438 Protein denotes IFNγ
T5807 17439-17449 Gene_expression denotes production
T5808 17550-17557 Protein denotes Pam3CYS
T13802 11679-11682 Protein denotes IL6
T13803 11688-11693 Protein denotes GMCSF
T13804 11912-11915 Protein denotes IL6
T13805 11981-11988 Protein denotes Sharpin
T2199 4755-4762 Protein denotes Sharpin
T2200 4777-4784 Protein denotes SHARPIN
T2201 4936-4943 Protein denotes SHARPIN
T2202 5150-5157 Protein denotes SHARPIN
T2203 5214-5221 Protein denotes SHARPIN
T2204 5284-5293 Negative_regulation denotes mutations
T2205 5307-5314 Protein denotes Sharpin
T2206 5524-5534 Transcription denotes expression
T2207 5538-5545 Protein denotes Sharpin
T2208 5678-5685 Protein denotes Sharpin
T2209 5695-5702 Transcription denotes present
T2210 5771-5780 Negative_regulation denotes decreased
T2211 5825-5834 Negative_regulation denotes reduction
T2212 5838-5845 Protein denotes Sharpin
T2213 5924-5931 Protein denotes Sharpin
T71 0-7 Protein denotes SHARPIN
T72 177-186 Negative_regulation denotes mutations
T73 194-201 Protein denotes Sharpin
T74 203-249 Protein denotes SHANK-associated RH domain-interacting protein
T75 266-272 Protein denotes Rbckl1
T76 274-279 Protein denotes Sipl1
T77 627-634 Protein denotes SHARPIN
T78 635-644 Negative_regulation denotes deficient
T79 749-756 Protein denotes SHARPIN
T80 758-768 Negative_regulation denotes deficiency
T81 1053-1057 Protein denotes CD40
T82 1059-1063 Protein denotes CD80
T83 1069-1073 Protein denotes CD86
T84 1095-1102 Protein denotes SHARPIN
T85 1103-1112 Negative_regulation denotes deficient
T86 1122-1129 Negative_regulation denotes reduced
T87 1122-1129 Negative_regulation denotes reduced
T88 1122-1129 Negative_regulation denotes reduced
T89 1122-1129 Negative_regulation denotes reduced
T90 1130-1143 Transcription denotes transcription
T91 1130-1143 Transcription denotes transcription
T92 1148-1157 Gene_expression denotes secretion
T12372 32776-32780 Protein denotes IKK1
T12373 32781-32782 Protein denotes 2
T12374 32793-32797 Protein denotes IκBα
T12375 32807-32811 Protein denotes IκBα
T12376 32823-32827 Protein denotes TBK1
T12377 32866-32870 Protein denotes ERK1
T12378 32871-32872 Protein denotes 2
T12379 32886-32890 Protein denotes ERK1
T12380 32891-32892 Protein denotes 2
T12381 32902-32912 Protein denotes Beta-actin
T13263 8308-8315 Protein denotes Sharpin
T13264 8316-8324 Negative_regulation denotes mutation
T14985 16393-16397 Protein denotes TBK1
T14986 16398-16402 Protein denotes IRF3
T14987 16404-16408 Protein denotes ERK1
T14988 16409-16410 Protein denotes 2
T14989 16440-16450 Protein denotes Beta-actin
T15325 18959-18968 Gene_expression denotes secretion true
T15326 18959-18968 Gene_expression denotes secretion true
T15327 18959-18968 Gene_expression denotes secretion true
T15328 18972-18976 Protein denotes IFNγ
T15329 18978-18981 Protein denotes IL4
T15330 18987-18990 Protein denotes IL2
T15331 19227-19237 Gene_expression denotes production true
T15332 19227-19237 Gene_expression denotes production true
T15333 19227-19237 Gene_expression denotes production true
T6683 19386-19400 Negative_regulation denotes null mutations
T6761 25310-25314 Protein denotes TNFα
T6762 25478-25485 Protein denotes SHARPIN
T6763 25599-25606 Protein denotes SHARPIN
T6764 25728-25735 Protein denotes Sharpin
T6765 25736-25745 Negative_regulation denotes deficient
T9628 26835-26842 Protein denotes Sharpin
T12082 31933-31940 Protein denotes SHARPIN
T4466 15840-15855 Phosphorylation denotes phosphorylation
T4467 15840-15855 Phosphorylation denotes phosphorylation
T4468 15840-15855 Phosphorylation denotes phosphorylation
T4469 15919-15926 Negative_regulation denotes absence
T4470 15941-15948 Protein denotes SHARPIN
T4471 15995-15999 Protein denotes TBK1
T4472 16000-16004 Protein denotes IRF3
T4473 16006-16010 Protein denotes ERK1
T4474 16011-16012 Protein denotes 2
T10948 29926-29935 Gene_expression denotes secretion true
T10949 29926-29935 Gene_expression denotes secretion true
T10950 29926-29935 Gene_expression denotes secretion true
T10951 29926-29935 Gene_expression denotes secretion true
T10952 29939-29943 Protein denotes IFNγ
T10953 29945-29948 Protein denotes IL4
T10954 29950-29953 Protein denotes IL2
T10955 29959-29964 Protein denotes IL17A
T10956 30173-30183 Gene_expression denotes production true
T10957 30173-30183 Gene_expression denotes production true
T10958 30173-30183 Gene_expression denotes production true
T10959 30173-30183 Gene_expression denotes production true
T3805 10426-10434 Positive_regulation denotes resulted
T3806 10426-10434 Positive_regulation denotes resulted
T3807 10438-10447 Gene_expression denotes secretion
T3808 10438-10447 Gene_expression denotes secretion
T3809 10451-10454 Protein denotes IL6
T3810 10469-10474 Protein denotes GMCSF
T3811 10532-10540 Gene_expression denotes produced
T3812 10532-10540 Gene_expression denotes produced
T3813 10555-10559 Positive_regulation denotes less true
T3814 10555-10559 Positive_regulation denotes less true
T3815 10815-10822 Protein denotes Sharpin
T3816 10823-10832 Negative_regulation denotes deficient
T3817 10870-10877 Protein denotes Sharpin
T3818 11091-11098 Protein denotes SHARPIN
T3819 11195-11198 Protein denotes Il6
T3820 11200-11207 Protein denotes Il12p40
T3821 11209-11214 Protein denotes Gmcsf
T3822 11220-11224 Protein denotes Ifnb
T14565 14284-14290 Negative_regulation denotes Normal true
T14566 14284-14290 Negative_regulation denotes Normal true
T14567 14291-14295 Protein denotes TLR4
T14568 14300-14303 Protein denotes MD2
T14569 14304-14314 Gene_expression denotes expression
T14570 14304-14314 Gene_expression denotes expression
T14571 14319-14328 Negative_regulation denotes decreased
T14572 14319-14328 Negative_regulation denotes decreased
T14573 14329-14333 Protein denotes IL10
T14574 14334-14343 Gene_expression denotes secretion
T14575 14348-14351 Protein denotes A20
T14576 14352-14362 Gene_expression denotes expression
T14577 14764-14768 Protein denotes IL10
T14578 14992-15002 Gene_expression denotes production true
T14579 15006-15009 Protein denotes A20
R4647 T5813 T5812 themeOf IL17A,production
R4648 T5815 T5816 themeOf IL2,production
R4649 T5817 T5818 themeOf induced,more
R4650 T5819 T5817 themeOf IL2,induced
R5387 T6719 T6718 themeOf Sharpin,deletion
R5388 T6722 T6723 themeOf Sharpin,expression
R5389 T6723 T6721 themeOf expression,absence
R5390 T6726 T6727 themeOf SHARPIN,deficient
R5391 T6730 T6731 themeOf Sharpin,mutation
R5392 T6734 T6732 themeOf TLR4,engage
R5393 T6735 T6733 themeOf TLR3,engage
R5394 T6737 T6736 themeOf TLR4,engages
R5395 T6740 T6741 themeOf TLR3,stimulated
R5396 T6743 T6744 themeOf Sharpin,mutation
R5397 T6747 T6748 themeOf SHARPIN,interacts
R5398 T6747 T6752 causeOf SHARPIN,linear-ubiquitin-chain-ligase activity
R5399 T6747 T6753 causeOf SHARPIN,linear-ubiquitin-chain-ligase activity
R5400 T6749 T6748 themeOf HOIP,interacts
R5401 T6752 T6751 themeOf linear-ubiquitin-chain-ligase activity,exerts
R5402 T6752 T6759 themeOf linear-ubiquitin-chain-ligase activity,disrupts
R5403 T6753 T6750 themeOf linear-ubiquitin-chain-ligase activity,exerts
R5404 T6753 T6760 themeOf linear-ubiquitin-chain-ligase activity,disrupts
R5405 T6754 T6752 themeOf RIP1,linear-ubiquitin-chain-ligase activity
R5406 T6755 T6753 themeOf NEMO,linear-ubiquitin-chain-ligase activity
R5407 T6757 T6758 themeOf Sharpin,null mutation
R5408 T6758 T6759 causeOf null mutation,disrupts
R5409 T6758 T6760 causeOf null mutation,disrupts
R5410 T6764 T6765 themeOf Sharpin,deficient
R8182 T9956 T9955 themeOf SHARPIN,express
R8183 T9958 T9959 themeOf SHARPIN,expression
R9076 T10953 T10950 themeOf IL4,secretion
R9077 T10954 T10958 themeOf IL2,production
R9078 T10954 T10948 themeOf IL2,secretion
R9079 T10955 T10959 themeOf IL17A,production
R9080 T10955 T10949 themeOf IL17A,secretion
R9392 T11339 T11338 themeOf Il6,expression
R9393 T11340 T11337 themeOf Il12p40,expression
R9394 T11341 T11336 themeOf Gmcsf,expression
R9395 T11342 T11335 themeOf Sharpin,expression
R11899 T14252 T14251 themeOf Il12p40,expression
R11900 T14253 T14250 themeOf Il6,expression
R11901 T14254 T14249 themeOf Gmcsf,expression
R11902 T14255 T14248 themeOf Ifnb,expression
R746 T956 T954 themeOf termination,resulting
R747 T957 T956 themeOf synthesis,termination
R748 T958 T955 themeOf absence,resulting
R749 T960 T959 themeOf secretion,decreased
R750 T961 T960 themeOf IFNγ,secretion
R751 T963 T962 themeOf IL5,Neutralization
R752 T964 T965 themeOf IL5,deficient
R753 T968 T969 themeOf SHARPIN,deficient
R754 T971 T972 themeOf SHARPIN,deficient
R755 T974 T973 themeOf SHARPIN,lack
R756 T976 T977 themeOf SHARPIN,deficient
R740 T950 T951 themeOf SHARPIN,expressed
R741 T952 T955 causeOf recessive mutations,resulting
R742 T952 T954 causeOf recessive mutations,resulting
R743 T953 T958 themeOf Sharpin,absence
R744 T953 T957 themeOf Sharpin,synthesis
R745 T953 T952 themeOf Sharpin,recessive mutations
R4633 T5790 T5789 themeOf SHARPIN,absence
R4634 T5792 T5793 themeOf IFNγ,production
R4635 T5793 T5791 themeOf production,elicited
R4636 T5794 T5796 themeOf concentration,lower
R4637 T5795 T5794 themeOf IFNγ,concentration
R4638 T5797 T5800 themeOf TLR3,agonists
R4639 T5798 T5799 themeOf 4,agonists
R4640 T5801 T5802 themeOf TLR2,ligand
R4641 T5803 T5802 themeOf Pam3CYS,ligand
R4642 T5804 T5805 causeOf Pam3CYS,induced
R4643 T5806 T5807 themeOf IFNγ,production
R4644 T5807 T5805 themeOf production,induced
R4645 T5810 T5811 themeOf IL4,produced
R4646 T5812 T5814 themeOf production,following
R1799 T2205 T2204 themeOf Sharpin,mutations
R1800 T2207 T2206 themeOf Sharpin,expression
R1801 T2208 T2209 themeOf Sharpin,present
R1802 T2209 T2210 themeOf present,decreased
R1803 T2212 T2211 themeOf Sharpin,reduction
R1804 T2214 T2215 locationOf cytoplasmic,localization
R1805 T2216 T2215 themeOf SHARPIN,localization
R36 T94 T90 themeOf IL6,transcription
R37 T94 T93 themeOf IL6,secretion
R38 T95 T91 themeOf GMCSF,transcription
R672 T74 T73 equivalentTo SHANK-associated RH domain-interacting protein,Sharpin
R674 T76 T73 equivalentTo Sipl1,Sharpin
R678 T85 T87 causeOf deficient,reduced
R679 T85 T89 causeOf deficient,reduced
R680 T85 T88 causeOf deficient,reduced
R681 T85 T86 causeOf deficient,reduced
R682 T90 T87 themeOf transcription,reduced
R683 T91 T89 themeOf transcription,reduced
R684 T92 T86 themeOf secretion,reduced
R35 T93 T88 themeOf secretion,reduced
R677 T84 T85 themeOf SHARPIN,deficient
R39 T95 T92 themeOf GMCSF,secretion
R40 T98 T96 equivalentTo ERK1,MAPK1
R41 T99 T97 equivalentTo 2,3
R42 T106 T105 themeOf Sharpin,loss
R671 T73 T72 themeOf Sharpin,mutations
R673 T75 T73 equivalentTo Rbckl1,Sharpin
R675 T77 T78 themeOf SHARPIN,deficient
R676 T79 T80 themeOf SHARPIN,deficiency
R10555 T12758 T12757 themeOf Sharpin,mRNA level
R10556 T12760 T12762 themeOf SHARPIN,localized
R10557 T12761 T12762 locationOf cytoplasm,localized
R11006 T13263 T13264 themeOf Sharpin,mutation
R12800 T15328 T15327 themeOf IFNγ,secretion
R12801 T15328 T15331 themeOf IFNγ,production
R12802 T15329 T15325 themeOf IL4,secretion
R12803 T15329 T15332 themeOf IL4,production
R12804 T15330 T15333 themeOf IL2,production
R12805 T15330 T15326 themeOf IL2,secretion
R5370 T6684 T6683 themeOf Sharpin,null mutations
R5371 T6686 T6685 themeOf SHARPIN,loss
R5372 T6687 T6688 themeOf SHARPIN,disruption
R5373 T6691 T6690 themeOf TNF,deletion
R5374 T6693 T6692 themeOf SHARPIN,deficiency
R5375 T6694 T6695 themeOf HMGB1,recognized
R5376 T6696 T6695 themeOf TLR2,recognized
R5377 T6698 T6701 themeOf IL12P35,deficient
R5378 T6699 T6700 themeOf IL12P40,deficient
R5379 T6703 T6712 themeOf activation,was not
R5380 T6704 T6714 themeOf activation,was not
R5381 T6705 T6711 themeOf activation,was not
R5382 T6706 T6713 themeOf activation,was not
R5383 T6707 T6706 themeOf TBK1,activation
R5384 T6708 T6705 themeOf IRF3,activation
R5385 T6709 T6703 themeOf ERK1,activation
R5386 T6710 T6704 themeOf 2,activation
R3595 T4413 T4412 equivalentTo MD2,myeloid differentiation factor 2
R3596 T4414 T4412 equivalentTo LY96,myeloid differentiation factor 2
R3597 T4418 T4416 themeOf CD14,expression
R3598 T4419 T4417 themeOf TLR4,expression
R3599 T4420 T4415 themeOf MD2,expression
R3600 T4422 T4421 themeOf IL10,secretion
R3601 T4424 T4423 themeOf levels,lower
R3602 T4425 T4424 themeOf IL10,levels
R3603 T4428 T4427 themeOf expression,Increased
R3604 T4429 T4428 themeOf A20,expression
R3605 T4430 T4432 themeOf transcript level,lower
R3606 T4431 T4430 themeOf A20,transcript level
R3607 T4434 T4433 themeOf A20,overexpression
R3608 T4441 T4442 themeOf Sharpin,deficient
R3609 T4447 T4446 themeOf phosphorylation,allowing
R3610 T4449 T4445 themeOf degradation,allowing
R3611 T4449 T4448 themeOf degradation,mediated
R3612 T4450 T4447 themeOf IκBα,phosphorylation
R3613 T4450 T4449 themeOf IκBα,degradation
R3614 T4451 T4460 themeOf phosphorylated,decreased
R3615 T4452 T4462 themeOf phosphorylated,decreased
R3616 T4453 T4461 themeOf phosphorylated,decreased
R3617 T4454 T4452 themeOf IKK1,phosphorylated
R3618 T4455 T4451 themeOf 2,phosphorylated
R3619 T4458 T4453 themeOf IκBα,phosphorylated
R3620 T4463 T4468 themeOf TBK1,phosphorylation
R3621 T4464 T4466 themeOf ERK1,phosphorylation
R3622 T4465 T4467 themeOf 2,phosphorylation
R3623 T4470 T4469 themeOf SHARPIN,absence
R9073 T10952 T10956 themeOf IFNγ,production
R9074 T10952 T10951 themeOf IFNγ,secretion
R9075 T10953 T10957 themeOf IL4,production
R3126 T3807 T3806 themeOf secretion,resulted
R3127 T3808 T3805 themeOf secretion,resulted
R3128 T3809 T3808 themeOf IL6,secretion
R3129 T3809 T3811 themeOf IL6,produced
R3130 T3810 T3807 themeOf GMCSF,secretion
R3131 T3810 T3812 themeOf GMCSF,produced
R3132 T3811 T3813 themeOf produced,less
R3133 T3812 T3814 themeOf produced,less
R3134 T3815 T3816 themeOf Sharpin,deficient
R12179 T14567 T14570 themeOf TLR4,expression
R12180 T14568 T14569 themeOf MD2,expression
R12181 T14569 T14566 themeOf expression,Normal
R12182 T14570 T14565 themeOf expression,Normal
R12183 T14573 T14574 themeOf IL10,secretion
R12184 T14574 T14571 themeOf secretion,decreased
R12185 T14575 T14576 themeOf A20,expression
R12186 T14576 T14572 themeOf expression,decreased
R12187 T14579 T14578 themeOf A20,production
R2686 T3243 T3242 themeOf increased,resulted
R2687 T3244 T3259 themeOf expression,increased
R2688 T3245 T3261 themeOf expression,increased
R2689 T3246 T3243 themeOf expression,increased
R2690 T3248 T3247 themeOf expression,difference
R2691 T3250 T3249 themeOf expression,difference
R2692 T3253 T3251 themeOf expression,difference
R2693 T3259 T3258 themeOf increased,resulted
R2694 T3261 T3260 themeOf increased,resulted
R2695 T3262 T3241 themeOf Sharpin,mutation
R2696 T3267 T3256 themeOf TLR3,ligand
R2697 T3268 T3257 themeOf TLR4,ligand
R2698 T3269 T3244 themeOf CD40,expression
R2699 T3269 T3248 themeOf CD40,expression
R2700 T3270 T3245 themeOf CD80,expression
R2701 T3270 T3250 themeOf CD80,expression
R2702 T3271 T3246 themeOf CD86,expression
R2703 T3271 T3253 themeOf CD86,expression
R2704 T3272 T3254 themeOf SHARPIN,deficiency
R2705 T3278 T3252 themeOf SHARPIN,deficiency
R2706 T3279 T3255 themeOf Sharpin,mutation

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T667 0-7 Q9H0F6 denotes SHARPIN
T668 194-201 Q9H0F6 denotes Sharpin
T669 274-279 Q9H0F6 denotes Sipl1
T670 627-634 Q9H0F6 denotes SHARPIN
T671 749-756 Q9H0F6 denotes SHARPIN
T672 1053-1057 P25942 denotes CD40
T673 1059-1063 P33681 denotes CD80
T674 1069-1073 P42081 denotes CD86
T675 1095-1102 Q9H0F6 denotes SHARPIN
T676 1188-1191 P05231 denotes IL6
T677 1202-1207 P04141 denotes GMCSF
T678 1296-1301 P28482 denotes MAPK1
T679 1305-1309 P27361 denotes ERK1
T680 1317-1323 Q15759 denotes MAPK11
T681 1334-1337 P53778 denotes p38
T682 1334-1337 Q16539 denotes p38
T683 1334-1337 Q15759 denotes p38
T684 1334-1337 O15264 denotes p38
T685 1363-1367 Q9UHD2 denotes TBK1
T686 1563-1566 P01730 denotes CD4
T687 1600-1607 Q9H0F6 denotes Sharpin
T1964 1771-1778 Q9H0F6 denotes SHARPIN
T1965 1997-2004 Q9H0F6 denotes Sharpin
T1966 3106-3109 P05113 denotes IL5
T1967 3148-3151 P05113 denotes IL5
T1968 3341-3348 Q9H0F6 denotes SHARPIN
T1969 3493-3500 Q9H0F6 denotes SHARPIN
T1970 4004-4007 P01730 denotes CD4
T1971 4215-4222 Q9H0F6 denotes SHARPIN
T1972 4284-4291 Q9H0F6 denotes SHARPIN
T1973 4497-4500 P01730 denotes CD4
T1974 4569-4576 Q9H0F6 denotes SHARPIN
T1975 4668-4675 Q9H0F6 denotes SHARPIN
T2688 4755-4762 Q9H0F6 denotes Sharpin
T2689 4777-4784 Q9H0F6 denotes SHARPIN
T2690 4936-4943 Q9H0F6 denotes SHARPIN
T2691 5150-5157 Q9H0F6 denotes SHARPIN
T2692 5214-5221 Q9H0F6 denotes SHARPIN
T2693 5307-5314 Q9H0F6 denotes Sharpin
T2694 5538-5545 Q9H0F6 denotes Sharpin
T2695 5678-5685 Q9H0F6 denotes Sharpin
T2696 5838-5845 Q9H0F6 denotes Sharpin
T2697 5924-5931 Q9H0F6 denotes Sharpin
T2698 6025-6032 Q9H0F6 denotes SHARPIN
T3615 7401-7408 Q9H0F6 denotes Sharpin
T3616 7536-7541 P20702 denotes CD11c
T3617 7552-7557 P20702 denotes CD11c
T3618 7596-7601 P20702 denotes CD11c
T3619 7753-7758 P20702 denotes CD11c
T3620 7827-7834 Q9H0F6 denotes SHARPIN
T3621 8162-8169 Q9H0F6 denotes Sharpin
T3622 9763-9768 P20702 denotes CD11c
T3623 9830-9834 P25942 denotes CD40
T3624 9836-9840 P33681 denotes CD80
T3625 9846-9850 P42081 denotes CD86
T3626 9856-9860 O15455 denotes TLR3
T3627 9885-9889 O00206 denotes TLR4
T3628 10092-10096 P25942 denotes CD40
T3629 10098-10102 P33681 denotes CD80
T3630 10108-10112 P42081 denotes CD86
T3631 10239-10246 Q9H0F6 denotes SHARPIN
T5355 15995-15999 Q9UHD2 denotes TBK1
T5356 16000-16004 Q14653 denotes IRF3
T5357 16006-16010 P27361 denotes ERK1
T5358 16018-16021 O15264 denotes p38
T5359 16018-16021 Q15759 denotes p38
T5360 16018-16021 Q16539 denotes p38
T5361 16018-16021 P53778 denotes p38
T6433 16862-16869 Q9H0F6 denotes SHARPIN
T6434 16988-16991 P01730 denotes CD4
T6435 17273-17277 O15455 denotes TLR3
T6436 17294-17298 O60603 denotes TLR2
T6437 17666-17670 O60603 denotes TLR2
T6438 17720-17723 P05112 denotes IL4
T6439 17898-17903 Q16552 denotes IL17A
T6440 18134-18137 P60568 denotes IL2
T6441 18237-18240 P60568 denotes IL2
T6442 18357-18360 P01730 denotes CD4
T9043 19414-19421 Q9H0F6 denotes Sharpin
T9044 19554-19561 Q9H0F6 denotes SHARPIN
T9045 20029-20036 Q9H0F6 denotes SHARPIN
T9046 20837-20840 P01375 denotes TNF
T9047 20881-20888 Q9H0F6 denotes SHARPIN
T9048 21379-21383 O60603 denotes TLR2
T9049 22008-22012 Q14653 denotes IRF3
T9050 22288-22292 Q9UHD2 denotes TBK1
T9051 22293-22297 Q14653 denotes IRF3
T9052 22299-22303 P27361 denotes ERK1
T9053 22310-22313 Q16539 denotes p38
T9054 22310-22313 P53778 denotes p38
T9055 22310-22313 O15264 denotes p38
T9056 22310-22313 Q15759 denotes p38
T9057 22730-22737 Q9H0F6 denotes SHARPIN
T9058 22892-22895 P21580 denotes A20
T9059 23507-23514 Q9H0F6 denotes Sharpin
T9060 23572-23579 Q9H0F6 denotes SHARPIN
T9061 23650-23657 Q9H0F6 denotes Sharpin
T9062 24010-24014 Q00653 denotes p100
T9063 24037-24040 Q00653 denotes p52
T9064 24066-24073 Q9H0F6 denotes SHARPIN
T9065 24137-24140 Q00653 denotes p52
T9066 24141-24145 Q01201 denotes RELB
T9067 24261-24268 Q9H0F6 denotes Sharpin
T9068 24421-24425 O00206 denotes TLR4
T9069 24430-24434 O15455 denotes TLR3
T9070 24484-24488 O00206 denotes TLR4
T9071 24654-24658 O00206 denotes TLR4
T9072 24724-24728 O15455 denotes TLR3
T9073 24862-24869 Q9H0F6 denotes Sharpin
T9074 24970-24974 Q13546 denotes RIP1
T9075 24979-24984 Q9Y4K3 denotes TRAF6
T9076 25024-25031 Q9H0F6 denotes SHARPIN
T9077 25047-25051 Q96EP0 denotes HOIP
T9078 25148-25152 Q13546 denotes RIP1
T9079 25157-25161 Q9Y6K9 denotes NEMO
T9080 25234-25241 Q9H0F6 denotes Sharpin
T9081 25478-25485 Q9H0F6 denotes SHARPIN
T9082 25599-25606 Q9H0F6 denotes SHARPIN
T9083 25728-25735 Q9H0F6 denotes Sharpin
T10284 27233-27240 Q9H0F6 denotes Sharpin
T10285 27414-27417 P21980 denotes TGC
T10286 27433-27440 Q9H0F6 denotes Sharpin
T10287 27566-27573 Q9H0F6 denotes SHARPIN
T10288 27705-27712 Q9H0F6 denotes SHARPIN
T10289 27896-27903 Q9H0F6 denotes SHARPIN
T10902 28588-28593 P20702 denotes CD11c
T10903 28642-28646 P25942 denotes CD40
T10904 28679-28683 P33681 denotes CD80
T11241 29716-29719 P01730 denotes CD4
T11242 29945-29948 P05112 denotes IL4
T11243 29950-29953 P60568 denotes IL2
T11244 29959-29964 Q16552 denotes IL17A
T11245 30060-30063 P01730 denotes CD4
T11246 30088-30091 P01730 denotes CD4
T11827 30503-30506 P05231 denotes Il6
T11828 30517-30522 P04141 denotes Gmcsf
T11829 30528-30535 Q9H0F6 denotes Sharpin
T11830 31218-31222 P60709 denotes Actb
T11831 31224-31227 P05231 denotes IL6
T11832 31244-31249 P04141 denotes Gmcsf
T11833 31255-31262 Q9H0F6 denotes Sharpin
T11834 31370-31377 P60709 denotes β-actin
T11835 31379-31387 Q04864 denotes Relative
T12608 32776-32780 O15111 denotes IKK1
T12609 32776-32780 Q60680-2 denotes IKK1
T12610 32793-32797 P25963 denotes IκBα
T12611 32807-32811 P25963 denotes IκBα
T12612 32823-32827 Q9UHD2 denotes TBK1
T12613 32838-32841 P53778 denotes p38
T12614 32838-32841 Q15759 denotes p38
T12615 32838-32841 Q16539 denotes p38
T12616 32838-32841 O15264 denotes p38
T12617 32851-32854 Q15759 denotes p38
T12618 32851-32854 O15264 denotes p38
T12619 32851-32854 P53778 denotes p38
T12620 32851-32854 Q16539 denotes p38
T12621 32866-32870 P27361 denotes ERK1
T12622 32886-32890 P27361 denotes ERK1
T12623 32902-32912 P60709 denotes Beta-actin
T13197 6129-6136 Q9H0F6 denotes SHARPIN
T13198 6355-6362 Q9H0F6 denotes SHARPIN
T13199 6730-6737 Q9H0F6 denotes Sharpin
T13200 6776-6784 Q04864 denotes relative
T13201 7007-7014 Q9H0F6 denotes SHARPIN
T13202 7183-7190 Q9H0F6 denotes SHARPIN
T13754 8308-8315 Q9H0F6 denotes Sharpin
T13755 8740-8745 P20702 denotes CD11c
T13756 8753-8758 P20702 denotes CD11c
T13757 8786-8791 P20702 denotes CD11c
T13758 8879-8884 P20702 denotes CD11c
T13759 8897-8902 P20702 denotes CD11c
T13760 8940-8945 P20702 denotes CD11c
T13761 9024-9029 P20702 denotes CD11c
T13762 9040-9045 P20702 denotes CD11c
T13763 9332-9336 P25942 denotes CD40
T13764 9343-9347 P33681 denotes CD80
T13765 9358-9362 P42081 denotes CD86
T13766 9456-9461 P20702 denotes CD11c
T14199 11679-11682 P05231 denotes IL6
T14200 11688-11693 P04141 denotes GMCSF
T14201 11912-11915 P05231 denotes IL6
T14202 11981-11988 Q9H0F6 denotes Sharpin
T14506 12714-12717 P05231 denotes Il6
T14507 12725-12730 P04141 denotes Gmcsf
T14853 14291-14295 O00206 denotes TLR4
T14854 14329-14333 P22301 denotes IL10
T14855 14348-14351 P21580 denotes A20
T14856 14453-14457 O00206 denotes TLR4
T14857 14470-14474 P08571 denotes CD14
T14858 14764-14768 P22301 denotes IL10
T14859 15006-15009 P21580 denotes A20
T15116 16393-16397 Q9UHD2 denotes TBK1
T15117 16398-16402 Q14653 denotes IRF3
T15118 16404-16408 P27361 denotes ERK1
T15119 16416-16419 O15264 denotes p38
T15120 16416-16419 P53778 denotes p38
T15121 16416-16419 Q16539 denotes p38
T15122 16416-16419 Q15759 denotes p38
T15123 16440-16450 P60709 denotes Beta-actin
T15124 16505-16509 O15111 denotes IKK1
T15125 16505-16509 Q60680-2 denotes IKK1
T15126 16518-16522 P25963 denotes IκBα
T15504 18563-18566 P01730 denotes CD4
T15505 18832-18835 P01730 denotes CD4
T15506 18978-18981 P05112 denotes IL4
T15507 18987-18990 P60568 denotes IL2
T15508 19091-19094 P01730 denotes CD4
T15509 19119-19122 P01730 denotes CD4
T4197 10451-10454 P05231 denotes IL6
T4198 10469-10474 P04141 denotes GMCSF
T4199 10815-10822 Q9H0F6 denotes Sharpin
T4200 10870-10877 Q9H0F6 denotes Sharpin
T4201 11091-11098 Q9H0F6 denotes SHARPIN
T4202 11195-11198 P05231 denotes Il6
T4203 11209-11214 P04141 denotes Gmcsf
T5321 13365-13369 O00206 denotes TLR4
T5322 13394-13398 P08571 denotes CD14
T5323 13514-13518 P08571 denotes CD14
T5324 13523-13527 O00206 denotes TLR4
T5325 13643-13647 P22301 denotes IL10
T5326 13802-13806 P22301 denotes IL10
T5327 13858-13862 P22301 denotes IL10
T5328 14002-14005 P21580 denotes A20
T5329 14082-14085 P21580 denotes A20
T5330 14188-14191 P21580 denotes A20
T5331 15035-15039 O15455 denotes TLR3
T5332 15154-15158 Q9UHD2 denotes TBK1
T5333 15159-15163 Q14653 denotes IRF3
T5334 15224-15228 Q9UHD2 denotes TBK1
T5335 15229-15233 Q14653 denotes IRF3
T5336 15319-15326 Q9H0F6 denotes Sharpin
T5337 15383-15393 Q60680-2 denotes IκB kinase
T5338 15383-15393 O15111 denotes IκB kinase
T5339 15395-15399 O15111 denotes IKK1
T5340 15395-15399 Q60680-2 denotes IKK1
T5341 15528-15532 P25963 denotes IκBα
T5342 15623-15627 O15111 denotes IKK1
T5343 15623-15627 Q60680-2 denotes IKK1
T5344 15633-15637 O15111 denotes IKK1
T5345 15633-15637 Q60680-2 denotes IKK1
T5346 15645-15649 P25963 denotes IκBα
T5347 15653-15657 P25963 denotes IκBα
T5348 15818-15822 Q9UHD2 denotes TBK1
T5349 15824-15828 P27361 denotes ERK1
T5350 15836-15839 O15264 denotes p38
T5351 15836-15839 P53778 denotes p38
T5352 15836-15839 Q16539 denotes p38
T5353 15836-15839 Q15759 denotes p38
T5354 15941-15948 Q9H0F6 denotes SHARPIN
T9924 26835-26842 Q9H0F6 denotes Sharpin
T10905 28716-28720 P42081 denotes CD86
T10906 28751-28755 P08571 denotes CD14
T10907 28799-28803 O00206 denotes TLR4
T10908 28860-28864 O00206 denotes TLR4
T10909 29116-29121 P20702 denotes CD11c
T12330 31933-31940 Q9H0F6 denotes SHARPIN

test2

Id Subject Object Predicate Lexical cue Negation Speculation
T50 1059-1063 Protein denotes CD80
T51 1069-1073 Protein denotes CD86
T52 1095-1102 Protein denotes SHARPIN
T53 1103-1112 Negative_regulation denotes deficient
T54 1122-1129 Negative_regulation denotes reduced
T55 1130-1143 Transcription denotes transcription
T56 1188-1191 Protein denotes IL6
T57 1202-1207 Protein denotes GMCSF
T58 1296-1301 Protein denotes MAPK1
T59 1302-1303 Protein denotes 3
T60 1305-1309 Protein denotes ERK1
T61 1310-1311 Protein denotes 2
T62 1317-1323 Protein denotes MAPK11
T63 1324-1326 Protein denotes 12
T64 1327-1329 Protein denotes 13
T65 1330-1332 Protein denotes 14
T66 1363-1367 Protein denotes TBK1
T67 1592-1596 Negative_regulation denotes loss
T68 1600-1607 Protein denotes Sharpin
T925 1771-1778 Protein denotes SHARPIN
T926 1997-2004 Protein denotes Sharpin
T927 2866-2875 Negative_regulation denotes decreased
T928 2876-2885 Gene_expression denotes secretion
T929 2889-2893 Protein denotes IFNγ
T930 3088-3102 Negative_regulation denotes Neutralization
T931 3106-3109 Protein denotes IL5
T932 3148-3151 Protein denotes IL5
T933 3152-3161 Negative_regulation denotes deficient
T934 3341-3348 Protein denotes SHARPIN
T935 3445-3449 Protein denotes TNFα
T936 3493-3500 Protein denotes SHARPIN
T937 3501-3510 Negative_regulation denotes deficient
T938 3563-3567 Protein denotes TNFα
T939 4215-4222 Protein denotes SHARPIN
T940 4223-4232 Negative_regulation denotes deficient
T941 4276-4280 Negative_regulation denotes lack
T942 4284-4291 Protein denotes SHARPIN
T943 4569-4576 Protein denotes SHARPIN
T944 4668-4675 Protein denotes SHARPIN
T945 4676-4685 Negative_regulation denotes deficient
T2184 4755-4762 Protein denotes Sharpin
T2185 4777-4784 Protein denotes SHARPIN
T2186 4936-4943 Protein denotes SHARPIN
T2187 5150-5157 Protein denotes SHARPIN
T2188 5214-5221 Protein denotes SHARPIN
T2189 5307-5314 Protein denotes Sharpin
T2190 5524-5534 Gene_expression denotes expression
T2191 5538-5545 Protein denotes Sharpin
T2192 5678-5685 Protein denotes Sharpin
T2193 5825-5834 Negative_regulation denotes reduction
T2194 5838-5845 Protein denotes Sharpin
T2195 5924-5931 Protein denotes Sharpin
T2196 6005-6017 Localization denotes localization
T2197 6025-6032 Protein denotes SHARPIN
T2835 7401-7408 Protein denotes Sharpin
T2836 7409-7417 Negative_regulation denotes mutation
T2837 7536-7541 Protein denotes CD11c
T2838 7542-7546 Protein denotes CD8α
T2839 7552-7557 Protein denotes CD11c
T2840 7558-7562 Protein denotes CD8α
T2841 7596-7601 Protein denotes CD11c
T2842 7602-7608 Protein denotes PDCA-1
T2843 7815-7823 Regulation denotes affected
T2844 7827-7834 Protein denotes SHARPIN
T2845 7835-7845 Negative_regulation denotes deficiency
T2846 8162-8169 Protein denotes Sharpin
T2847 8170-8178 Negative_regulation denotes mutation
T2848 9763-9768 Protein denotes CD11c
T2849 9791-9801 Gene_expression denotes expression true
T2850 9830-9834 Protein denotes CD40
T2851 9836-9840 Protein denotes CD80 true
T2852 9846-9850 Protein denotes CD86
T2853 9856-9860 Protein denotes TLR3 true
T2854 9885-9889 Protein denotes TLR4
T2855 9890-9896 Binding denotes ligand
T2856 10056-10064 Positive_regulation denotes resulted
T2857 10068-10077 Positive_regulation denotes increased
T2858 10078-10088 Gene_expression denotes expression
T2859 10092-10096 Protein denotes CD40
T2860 10098-10102 Protein denotes CD80
T2861 10108-10112 Protein denotes CD86
T2862 10239-10246 Protein denotes SHARPIN
T2863 10247-10257 Negative_regulation denotes deficiency
T3791 10385-10393 Negative_regulation denotes impaired
T3792 10426-10434 Positive_regulation denotes resulted
T3793 10438-10447 Gene_expression denotes secretion
T3794 10451-10454 Protein denotes IL6
T3795 10469-10474 Protein denotes GMCSF
T3796 10815-10822 Protein denotes Sharpin
T3797 10823-10832 Negative_regulation denotes deficient true
T3798 10870-10877 Protein denotes Sharpin true
T3799 11083-11087 Regulation denotes role
T3800 11091-11098 Protein denotes SHARPIN
T3801 11195-11198 Protein denotes Il6
T3802 11200-11207 Protein denotes Il12p40
T3803 11209-11214 Protein denotes Gmcsf
T3804 11220-11224 Protein denotes Ifnb
T4358 13365-13369 Protein denotes TLR4
T4359 13394-13398 Protein denotes CD14
T4360 13403-13435 Protein denotes myeloid differentiation factor 2
T4361 13437-13440 Protein denotes MD2 true
T4362 13441-13445 Protein denotes LY96 true
T4363 13493-13503 Gene_expression denotes expression
T4364 13514-13518 Protein denotes CD14
T4365 13523-13527 Protein denotes TLR4
T4366 13528-13531 Protein denotes MD2
T4367 13643-13647 Protein denotes IL10 true
T4368 13802-13806 Protein denotes IL10
T4369 13858-13862 Protein denotes IL10
T4370 14002-14005 Protein denotes A20
T4371 14062-14072 Transcription denotes transcript
T4372 14082-14085 Protein denotes A20
T4373 14170-14184 Positive_regulation denotes overexpression
T4374 14188-14191 Protein denotes A20
T4375 15035-15039 Protein denotes TLR3
T4376 15040-15041 Protein denotes 4
T4377 15091-15100 Regulation denotes regulated
T4378 15154-15158 Protein denotes TBK1
T4379 15159-15163 Protein denotes IRF3
T4380 15224-15228 Protein denotes TBK1
T4381 15229-15233 Protein denotes IRF3
T4382 15319-15326 Protein denotes Sharpin
T4383 15327-15336 Negative_regulation denotes deficient
T4384 15395-15399 Protein denotes IKK1
T4385 15400-15401 Protein denotes 2
T4386 15453-15468 Phosphorylation denotes phosphorylation
T4387 15493-15504 Protein_catabolism denotes degradation
T4388 15528-15532 Protein denotes IκBα
T4389 15536-15543 Localization denotes release
T4390 15608-15622 Phosphorylation denotes phosphorylated
T4391 15623-15627 Protein denotes IKK1
T4392 15628-15629 Protein denotes 2
T4393 15633-15637 Protein denotes IKK1
T4394 15638-15639 Protein denotes 2
T4395 15645-15649 Protein denotes IκBα
T4396 15653-15657 Protein denotes IκBα
T4397 15659-15668 Positive_regulation denotes following
T4398 15714-15723 Negative_regulation denotes decreased
T4399 15818-15822 Protein denotes TBK1
T4400 15824-15828 Protein denotes ERK1
T4401 15829-15830 Protein denotes 2
T4402 15840-15855 Phosphorylation denotes phosphorylation
T4403 15919-15926 Negative_regulation denotes absence
T4404 15941-15948 Protein denotes SHARPIN
T4405 15988-15994 Regulation denotes affect
T4406 15995-15999 Protein denotes TBK1
T4407 16000-16004 Protein denotes IRF3
T4408 16006-16010 Protein denotes ERK1
T4409 16011-16012 Protein denotes 2
T5763 16851-16858 Negative_regulation denotes absence
T5764 16862-16869 Protein denotes SHARPIN
T5765 17046-17054 Positive_regulation denotes elicited
T5766 17062-17066 Protein denotes IFNγ
T5767 17067-17077 Gene_expression denotes production true
T5768 17091-17104 Gene_expression denotes concentration
T5769 17108-17112 Protein denotes IFNγ
T5770 17273-17277 Protein denotes TLR3
T5771 17278-17279 Protein denotes 4
T5772 17294-17298 Protein denotes TLR2
T5773 17299-17305 Binding denotes ligand
T5774 17306-17313 Protein denotes Pam3CYS
T5775 17395-17402 Protein denotes Pam3CYS
T5776 17419-17426 Positive_regulation denotes induced
T5777 17434-17438 Protein denotes IFNγ
T5778 17439-17449 Gene_expression denotes production
T5779 17550-17557 Protein denotes Pam3CYS
T5780 17656-17665 Positive_regulation denotes following
T5781 17666-17670 Protein denotes TLR2
T5782 17720-17723 Protein denotes IL4
T5783 17861-17871 Gene_expression denotes production
T5784 17898-17903 Protein denotes IL17A
T5785 17904-17913 Positive_regulation denotes following
T5786 18134-18137 Protein denotes IL2
T5787 18138-18148 Gene_expression denotes production
T5788 18237-18240 Protein denotes IL2
T9627 26835-26842 Protein denotes Sharpin
T9946 27233-27240 Protein denotes Sharpin
T9947 27433-27440 Protein denotes Sharpin true
T9948 27546-27553 Gene_expression denotes express
T9949 27566-27573 Protein denotes SHARPIN
T9950 27705-27712 Protein denotes SHARPIN
T9951 27896-27903 Protein denotes SHARPIN
T9952 27904-27914 Gene_expression denotes expression
T11321 30218-30228 Transcription denotes expression true
T11322 30489-30499 Gene_expression denotes expression true
T11323 30503-30506 Protein denotes Il6 true
T11324 30508-30515 Protein denotes Il12p40 true
T11325 30517-30522 Protein denotes Gmcsf
T11326 30528-30535 Protein denotes Sharpin
T11327 30856-30883 Protein denotes M-MLV Reverse Transcriptase
T11328 31218-31222 Protein denotes Actb
T11329 31224-31227 Protein denotes IL6
T11330 31229-31236 Protein denotes IL12p40
T11331 31238-31242 Protein denotes Ifnβ
T11332 31244-31249 Protein denotes Gmcsf
T11333 31255-31262 Protein denotes Sharpin
T11334 31370-31377 Protein denotes β-actin
T12081 31933-31940 Protein denotes SHARPIN
T12362 32776-32780 Protein denotes IKK1
T12363 32781-32782 Protein denotes 2
T12364 32793-32797 Protein denotes IκBα
T12365 32807-32811 Protein denotes IκBα
T12366 32823-32827 Protein denotes TBK1
T12367 32866-32870 Protein denotes ERK1
T12368 32871-32872 Protein denotes 2
T12369 32886-32890 Protein denotes ERK1
T12370 32891-32892 Protein denotes 2
T12371 32902-32912 Protein denotes Beta-actin
T12745 6129-6136 Protein denotes SHARPIN true
T12746 6355-6362 Protein denotes SHARPIN
T12747 6716-6726 Transcription denotes mRNA level
T12748 6730-6737 Protein denotes Sharpin
T12749 6792-6807 Transcription denotes mRNA expression
T12750 6982-6992 Gene_expression denotes expression
T12751 7007-7014 Protein denotes SHARPIN
T12752 7183-7190 Protein denotes SHARPIN
T12753 7217-7226 Localization denotes localized
T13259 8298-8304 Regulation denotes Effect
T13260 8308-8315 Protein denotes Sharpin
T13261 8316-8324 Negative_regulation denotes mutation
T13798 11679-11682 Protein denotes IL6
T13799 11688-11693 Protein denotes GMCSF
T13800 11912-11915 Protein denotes IL6
T13801 11981-11988 Protein denotes Sharpin
T14242 12673-12680 Regulation denotes measure true
T14243 12685-12695 Gene_expression denotes expression true
T14244 12699-12706 Protein denotes Il12p40 true
T14245 12714-12717 Protein denotes Il6 true
T14246 12725-12730 Protein denotes Gmcsf
T14247 12742-12746 Protein denotes Ifnb
T14554 14291-14295 Protein denotes TLR4 true
T14555 14300-14303 Protein denotes MD2
T14556 14304-14314 Gene_expression denotes expression true
T14557 14319-14328 Negative_regulation denotes decreased
T14558 14329-14333 Protein denotes IL10
T14559 14334-14343 Localization denotes secretion
T14560 14348-14351 Protein denotes A20
T14561 14352-14362 Gene_expression denotes expression
T14562 14764-14768 Protein denotes IL10 true
T14563 14992-15002 Gene_expression denotes production
T14564 15006-15009 Protein denotes A20
T14977 16393-16397 Protein denotes TBK1
T14978 16398-16402 Protein denotes IRF3
T14979 16404-16408 Protein denotes ERK1
T14980 16409-16410 Protein denotes 2
T14981 16440-16450 Protein denotes Beta-actin
T14982 16505-16509 Protein denotes IKK1
T14983 16510-16511 Protein denotes 2
T14984 16518-16522 Protein denotes IκBα
T37 0-7 Protein denotes SHARPIN
T38 177-186 Negative_regulation denotes mutations
T39 194-201 Protein denotes Sharpin
T40 203-249 Protein denotes SHANK-associated RH domain-interacting protein
T41 266-272 Protein denotes Rbckl1
T42 274-279 Protein denotes Sipl1
T43 627-634 Protein denotes SHARPIN
T44 635-644 Negative_regulation denotes deficient
T45 749-756 Protein denotes SHARPIN
T46 758-768 Negative_regulation denotes deficiency
T47 1001-1010 Positive_regulation denotes increases
T48 1014-1024 Gene_expression denotes expression
T49 1053-1057 Protein denotes CD40
R1796 T2191 T2190 themeOf Sharpin,expression
R1797 T2194 T2193 themeOf Sharpin,reduction
R1798 T2197 T2196 themeOf SHARPIN,localization
R2318 T2835 T2836 themeOf Sharpin,mutation
R2319 T2844 T2845 themeOf SHARPIN,deficiency
R2320 T2845 T2843 causeOf deficiency,affected
R2321 T2846 T2847 themeOf Sharpin,mutation
R2322 T2853 T2855 themeOf TLR3,ligand
R2323 T2854 T2855 themeOf TLR4,ligand
R2324 T2857 T2856 themeOf increased,resulted
R2325 T2858 T2857 themeOf expression,increased
R2326 T2859 T2858 themeOf CD40,expression
R2327 T2860 T2858 themeOf CD80,expression
R2328 T2861 T2858 themeOf CD86,expression
R2329 T2862 T2863 themeOf SHARPIN,deficiency
R3122 T3793 T3792 themeOf secretion,resulted
R3123 T3794 T3793 themeOf IL6,secretion
R3124 T3795 T3793 themeOf GMCSF,secretion
R3125 T3796 T3797 themeOf Sharpin,deficient
R3576 T4361 T4360 equivalentTo MD2,myeloid differentiation factor 2
R3577 T4362 T4360 equivalentTo LY96,myeloid differentiation factor 2
R3578 T4364 T4363 themeOf CD14,expression
R3579 T4365 T4363 themeOf TLR4,expression
R3580 T4366 T4363 themeOf MD2,expression
R3581 T4372 T4371 themeOf A20,transcript
R3582 T4374 T4373 themeOf A20,overexpression
R3583 T4382 T4383 themeOf Sharpin,deficient
R3584 T4388 T4386 themeOf IκBα,phosphorylation
R3585 T4388 T4389 themeOf IκBα,release
R3586 T4388 T4387 themeOf IκBα,degradation
R3587 T4391 T4390 themeOf IKK1,phosphorylated
R3588 T4392 T4390 themeOf 2,phosphorylated
R3589 T4395 T4390 themeOf IκBα,phosphorylated
R3590 T4399 T4402 themeOf TBK1,phosphorylation
R3591 T4400 T4402 themeOf ERK1,phosphorylation
R3592 T4401 T4402 themeOf 2,phosphorylation
R3593 T4403 T4405 causeOf absence,affect
R3594 T4404 T4403 themeOf SHARPIN,absence
R4621 T5764 T5763 themeOf SHARPIN,absence
R4622 T5766 T5767 themeOf IFNγ,production
R4623 T5767 T5765 themeOf production,elicited
R4624 T5769 T5768 themeOf IFNγ,concentration
R4625 T5772 T5773 themeOf TLR2,ligand
R4626 T5774 T5773 themeOf Pam3CYS,ligand
R4627 T5775 T5776 causeOf Pam3CYS,induced
R4628 T5777 T5778 themeOf IFNγ,production
R4629 T5778 T5776 themeOf production,induced
R4630 T5783 T5785 themeOf production,following
R4631 T5784 T5783 themeOf IL17A,production
R4632 T5786 T5787 themeOf IL2,production
R9388 T11323 T11322 themeOf Il6,expression
R9390 T11325 T11322 themeOf Gmcsf,expression
R9391 T11326 T11322 themeOf Sharpin,expression
R10552 T12748 T12749 themeOf Sharpin,mRNA expression
R10553 T12748 T12747 themeOf Sharpin,mRNA level
R10554 T12752 T12753 themeOf SHARPIN,localized
R11005 T13260 T13261 themeOf Sharpin,mutation
R11894 T14243 T14242 themeOf expression,measure
R11895 T14244 T14243 themeOf Il12p40,expression
R11896 T14245 T14243 themeOf Il6,expression
R11897 T14246 T14243 themeOf Gmcsf,expression
R11898 T14247 T14243 themeOf Ifnb,expression
R12172 T14554 T14556 themeOf TLR4,expression
R12173 T14555 T14556 themeOf MD2,expression
R12174 T14558 T14559 themeOf IL10,secretion
R12175 T14559 T14557 themeOf secretion,decreased
R12176 T14560 T14561 themeOf A20,expression
R12177 T14561 T14557 themeOf expression,decreased
R12178 T14564 T14563 themeOf A20,production
R662 T39 T38 themeOf Sharpin,mutations
R663 T40 T39 equivalentTo SHANK-associated RH domain-interacting protein,Sharpin
R664 T41 T39 equivalentTo Rbckl1,Sharpin
R665 T42 T38 themeOf Sipl1,mutations
R666 T42 T39 equivalentTo Sipl1,Sharpin
R667 T43 T44 themeOf SHARPIN,deficient
R668 T45 T46 themeOf SHARPIN,deficiency
R669 T48 T47 themeOf expression,increases
R670 T49 T48 themeOf CD40,expression
R732 T928 T927 themeOf secretion,decreased
R733 T929 T928 themeOf IFNγ,secretion
R734 T931 T930 themeOf IL5,Neutralization
R735 T932 T933 themeOf IL5,deficient
R736 T936 T937 themeOf SHARPIN,deficient
R737 T939 T940 themeOf SHARPIN,deficient
R738 T942 T941 themeOf SHARPIN,lack
R739 T944 T945 themeOf SHARPIN,deficient
R23 T50 T48 themeOf CD80,expression
R24 T51 T48 themeOf CD86,expression
R25 T52 T53 themeOf SHARPIN,deficient
R26 T53 T54 causeOf deficient,reduced
R27 T55 T54 themeOf transcription,reduced
R28 T56 T55 themeOf IL6,transcription
R29 T57 T55 themeOf GMCSF,transcription
R30 T60 T58 equivalentTo ERK1,MAPK1
R31 T60 T59 equivalentTo ERK1,3
R32 T61 T59 equivalentTo 2,3
R33 T61 T58 equivalentTo 2,MAPK1
R34 T68 T67 themeOf Sharpin,loss
R8180 T9949 T9948 themeOf SHARPIN,express
R8181 T9951 T9952 themeOf SHARPIN,expression
R9389 T11324 T11322 themeOf Il12p40,expression