PMC:3265313 / 5097-6069 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3265313","sourcedb":"PMC","sourceid":"3265313","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3265313","text":"2.4. Effect of Drying, Heat, and Relative Humidity\nTen microlitre of maintenance medium containing 107 TCID50 per mL of virus was placed in individual wells of a 24-well plastic plates and allowed to dry at room temperature (22~25°C) and relative humidity of 40–50% (i.e., conditions prevailing in a typical air-conditioned room). One hundred microlitre of MM was used to resuspend the virus at 0 hr, 3 hr, 7 hr, 11 hr, 13 hr, 24 hr, and up to 4 weeks and the residual virus infectivity was titrated. Controls in closed screw cap eppendorf tube were included each time and treated similarly but without drying.\nThe experiment was repeated at different temperatures (38°C, 33°C, 28°C) and relative humidities (\u003e95%, 80~89%) for 3 hr, 7 hr, 11 hr, 13 hr, and 24 hr. A nebulizer under a controlled condition was used to generate high and relative low humidity environment. All the experiments above were conducted in duplicate and the residual viral infectivity was titrated.","divisions":[{"label":"title","span":{"begin":0,"end":50}},{"label":"p","span":{"begin":51,"end":610}}],"tracks":[]}