PMC:3245220 / 541-1876
Annnotations
bionlp-st-ge-2016-uniprot
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T451 | 166-170 | P17252 | denotes | PKCα |
| T452 | 228-232 | P17252 | denotes | PKCα |
| T453 | 248-252 | P17252 | denotes | PKCα |
| T454 | 297-302 | P09603 | denotes | M-CSF |
| T455 | 299-302 | P04141 | denotes | CSF |
| T456 | 394-399 | P09603 | denotes | M-CSF |
| T457 | 396-399 | P04141 | denotes | CSF |
| T458 | 484-488 | P17252 | denotes | PKCα |
| T459 | 523-528 | P09603 | denotes | M-CSF |
| T460 | 525-528 | P04141 | denotes | CSF |
| T461 | 610-613 | Q04206 | denotes | p65 |
| T462 | 610-613 | P21579 | denotes | p65 |
| T463 | 690-693 | Q04206 | denotes | p65 |
| T464 | 690-693 | P21579 | denotes | p65 |
| T465 | 738-743 | P09603 | denotes | M-CSF |
| T466 | 740-743 | P04141 | denotes | CSF |
| T3308 | 968-1004 | P09603 | denotes | Macrophage colony-stimulating factor |
| T3309 | 979-1004 | P04141 | denotes | colony-stimulating factor |
| T3310 | 1006-1011 | P09603 | denotes | M-CSF |
| T3311 | 1008-1011 | P04141 | denotes | CSF |
| T3312 | 1093-1098 | P09603 | denotes | M-CSF |
| T3313 | 1095-1098 | P04141 | denotes | CSF |
bionlp-st-ge-2016-reference
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T468 | 173-182 | Negative_regulation | denotes | inhibitor |
| T469 | 173-182 | Negative_regulation | denotes | inhibitor |
| T470 | 192-206 | Gene_expression | denotes | overexpression |
| T471 | 330-337 | Negative_regulation | denotes | reduced |
| T472 | 345-360 | Phosphorylation | denotes | phosphorylation |
| T473 | 492-501 | Regulation | denotes | important |
| T474 | 529-536 | Positive_regulation | denotes | induced |
| T475 | 621-636 | Phosphorylation | denotes | phosphorylation |
| T478 | 338-344 | Entity | denotes | Ser276 |
| T479 | 614-620 | Entity | denotes | Ser276 |
| T480 | 166-170 | Protein | denotes | PKCα |
| T487 | 297-302 | Protein | denotes | M-CSF |
| T488 | 394-399 | Protein | denotes | M-CSF |
| T489 | 523-528 | Protein | denotes | M-CSF |
| T490 | 738-743 | Protein | denotes | M-CSF |
| T491 | 171-172 | Protein | denotes | β |
| T493 | 694-700 | Entity | denotes | Ser276 |
| T494 | 210-232 | Protein | denotes | dominant negative PKCα |
| T495 | 248-258 | Protein | denotes | PKCα siRNA |
| T496 | 369-372 | Protein | denotes | p65 |
| T497 | 484-488 | Protein | denotes | PKCα |
| T498 | 610-613 | Protein | denotes | p65 |
| T499 | 690-693 | Protein | denotes | p65 |
| T3526 | 968-1004 | Protein | denotes | Macrophage colony-stimulating factor |
| T3527 | 1006-1011 | Protein | denotes | M-CSF |
| T3529 | 1093-1098 | Protein | denotes | M-CSF |
| R402 | T472 | T471 | themeOf | phosphorylation,reduced |
| R403 | T474 | T473 | themeOf | induced,important |
| R404 | T475 | T474 | themeOf | phosphorylation,induced |
| R407 | T478 | T472 | themeOf | Ser276,phosphorylation |
| R408 | T478 | T496 | partOf | Ser276,p65 |
| R409 | T479 | T475 | themeOf | Ser276,phosphorylation |
| R410 | T479 | T498 | partOf | Ser276,p65 |
| R411 | T480 | T468 | themeOf | PKCα,inhibitor |
| R415 | T491 | T469 | themeOf | β,inhibitor |
| R416 | T493 | T499 | partOf | Ser276,p65 |
| R417 | T494 | T470 | themeOf | dominant negative PKCα,overexpression |
| R2985 | T3527 | T3526 | equivalentTo | M-CSF,Macrophage colony-stimulating factor |
bionlp-st-ge-2016-reference-tees
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T747 | 96-120 | Protein | denotes | general protein kinase C |
| T748 | 122-125 | Protein | denotes | PKC |
| T749 | 166-172 | Protein | denotes | PKCα/β |
| T750 | 210-243 | Protein | denotes | dominant negative PKCα constructs |
| T751 | 248-266 | Protein | denotes | PKCα siRNA reduced |
| T752 | 267-272 | Protein | denotes | NF-κB |
| T753 | 297-302 | Protein | denotes | M-CSF |
| T754 | 192-206 | Gene_expression | denotes | overexpression |
| T755 | 192-206 | Gene_expression | denotes | overexpression |
| T756 | 192-206 | Gene_expression | denotes | overexpression |
| T757 | 192-206 | Gene_expression | denotes | overexpression |
| T758 | 285-293 | Positive_regulation | denotes | response |
| T759 | 285-293 | Positive_regulation | denotes | response |
| T760 | 285-293 | Positive_regulation | denotes | response |
| T761 | 285-293 | Positive_regulation | denotes | response |
| T762 | 285-293 | Positive_regulation | denotes | response |
| T763 | 364-372 | Protein | denotes | NF-κBp65 |
| T764 | 394-399 | Protein | denotes | M-CSF |
| T765 | 338-344 | Entity | denotes | Ser276 |
| T766 | 345-360 | Phosphorylation | denotes | phosphorylation |
| T767 | 384-393 | Negative_regulation | denotes | decreased |
| T768 | 330-337 | Negative_regulation | denotes | reduced |
| T769 | 468-472 | Protein | denotes | PKCs |
| T770 | 484-488 | Protein | denotes | PKCα |
| T771 | 523-528 | Protein | denotes | M-CSF |
| T772 | 537-542 | Protein | denotes | NF-κB |
| T773 | 571-576 | Protein | denotes | NF-κB |
| T774 | 604-613 | Protein | denotes | NF-κB p65 |
| T775 | 614-620 | Protein | denotes | Ser276 |
| T776 | 621-636 | Phosphorylation | denotes | phosphorylation |
| T777 | 621-636 | Phosphorylation | denotes | phosphorylation |
| T778 | 511-518 | Positive_regulation | denotes | kinases |
| T779 | 511-518 | Positive_regulation | denotes | kinases |
| T780 | 684-693 | Protein | denotes | NF-κB p65 |
| T781 | 694-700 | Protein | denotes | Ser276 |
| T782 | 738-743 | Protein | denotes | M-CSF |
| T783 | 755-760 | Protein | denotes | NF-κB |
| T784 | 761-771 | Positive_regulation | denotes | activation |
| T785 | 744-754 | Positive_regulation | denotes | stimulated |
| T786 | 761-771 | Positive_regulation | denotes | activation |
| T4393 | 968-1004 | Protein | denotes | Macrophage colony-stimulating factor |
| T4394 | 1006-1011 | Protein | denotes | M-CSF |
| T4395 | 1093-1098 | Protein | denotes | M-CSF |
| T4396 | 1316-1321 | Protein | denotes | NF-κB |
| T4398 | 1322-1332 | Positive_regulation | denotes | activation |
| R525 | T747 | T754 | themeOf | general protein kinase C,overexpression |
| R526 | T748 | T755 | themeOf | PKC,overexpression |
| R527 | T750 | T756 | themeOf | dominant negative PKCα constructs,overexpression |
| R528 | T751 | T757 | themeOf | PKCα siRNA reduced,overexpression |
| R529 | T751 | T758 | causeOf | PKCα siRNA reduced,response |
| R530 | T751 | T759 | causeOf | PKCα siRNA reduced,response |
| R531 | T751 | T760 | causeOf | PKCα siRNA reduced,response |
| R532 | T751 | T761 | causeOf | PKCα siRNA reduced,response |
| R533 | T751 | T762 | causeOf | PKCα siRNA reduced,response |
| R534 | T753 | T758 | themeOf | M-CSF,response |
| R535 | T754 | T759 | themeOf | overexpression,response |
| R536 | T755 | T760 | themeOf | overexpression,response |
| R537 | T756 | T761 | themeOf | overexpression,response |
| R538 | T757 | T762 | themeOf | overexpression,response |
| R539 | T763 | T766 | themeOf | NF-κBp65,phosphorylation |
| R540 | T763 | T765 | partOf | NF-κBp65,Ser276 |
| R541 | T765 | T766 | Site | Ser276,phosphorylation |
| R542 | T766 | T767 | themeOf | phosphorylation,decreased |
| R543 | T766 | T768 | themeOf | phosphorylation,reduced |
| R544 | T770 | T778 | causeOf | PKCα,kinases |
| R545 | T770 | T779 | causeOf | PKCα,kinases |
| R546 | T774 | T776 | themeOf | NF-κB p65,phosphorylation |
| R547 | T775 | T777 | themeOf | Ser276,phosphorylation |
| R548 | T776 | T778 | themeOf | phosphorylation,kinases |
| R549 | T777 | T779 | themeOf | phosphorylation,kinases |
| R550 | T782 | T785 | causeOf | M-CSF,stimulated |
| R551 | T783 | T784 | themeOf | NF-κB,activation |
| R552 | T784 | T785 | themeOf | activation,stimulated |
| R553 | T785 | T786 | themeOf | stimulated,activation |
| R3330 | T4396 | T4398 | themeOf | NF-κB,activation |
events-check-again
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T705 | 166-170 | Protein | denotes | PKCα |
| T706 | 171-172 | Protein | denotes | β |
| T707 | 173-182 | Negative_regulation | denotes | inhibitor |
| T708 | 173-182 | Negative_regulation | denotes | inhibitor |
| T709 | 192-206 | Gene_expression | denotes | overexpression |
| T710 | 210-232 | Protein | denotes | dominant negative PKCα |
| T711 | 248-258 | Protein | denotes | PKCα siRNA |
| T712 | 297-302 | Protein | denotes | M-CSF |
| T713 | 330-337 | Negative_regulation | denotes | reduced |
| T714 | 338-344 | Entity | denotes | Ser276 |
| T715 | 345-360 | Phosphorylation | denotes | phosphorylation |
| T716 | 369-372 | Protein | denotes | p65 |
| T717 | 394-399 | Protein | denotes | M-CSF |
| T718 | 484-488 | Protein | denotes | PKCα |
| T719 | 492-501 | Regulation | denotes | important |
| T720 | 523-528 | Protein | denotes | M-CSF |
| T721 | 529-536 | Positive_regulation | denotes | induced |
| T722 | 610-613 | Protein | denotes | p65 |
| T723 | 614-620 | Entity | denotes | Ser276 |
| T724 | 621-636 | Phosphorylation | denotes | phosphorylation |
| T725 | 690-693 | Protein | denotes | p65 |
| T726 | 694-700 | Entity | denotes | Ser276 |
| T727 | 738-743 | Protein | denotes | M-CSF |
| T4262 | 968-1004 | Protein | denotes | Macrophage colony-stimulating factor |
| T4263 | 1006-1011 | Protein | denotes | M-CSF |
| T4264 | 1093-1098 | Protein | denotes | M-CSF |
| R501 | T705 | T707 | themeOf | PKCα,inhibitor |
| R502 | T706 | T708 | themeOf | β,inhibitor |
| R503 | T710 | T709 | themeOf | dominant negative PKCα,overexpression |
| R504 | T714 | T715 | themeOf | Ser276,phosphorylation |
| R505 | T714 | T716 | partOf | Ser276,p65 |
| R506 | T715 | T713 | themeOf | phosphorylation,reduced |
| R507 | T721 | T719 | themeOf | induced,important |
| R508 | T723 | T724 | themeOf | Ser276,phosphorylation |
| R509 | T723 | T722 | partOf | Ser276,p65 |
| R510 | T724 | T721 | themeOf | phosphorylation,induced |
| R511 | T726 | T725 | partOf | Ser276,p65 |
| R3218 | T4263 | T4262 | equivalentTo | M-CSF,Macrophage colony-stimulating factor |
ICD10
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T3588 | 1195-1210 | http://purl.bioontology.org/ontology/ICD10/I70 | denotes | atherosclerosis |
GO-CC
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T533 | 71-75 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cell |
| T3618 | 1108-1112 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cell |
GO-MF
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T528 | 122-125 | http://purl.obolibrary.org/obo/GO_0004697 | denotes | PKC |
| T529 | 166-170 | http://purl.obolibrary.org/obo/GO_0004697 | denotes | PKCα |
| T530 | 228-232 | http://purl.obolibrary.org/obo/GO_0004697 | denotes | PKCα |
| T531 | 248-252 | http://purl.obolibrary.org/obo/GO_0004697 | denotes | PKCα |
| T532 | 484-488 | http://purl.obolibrary.org/obo/GO_0004697 | denotes | PKCα |
GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T509 | 345-360 | http://purl.obolibrary.org/obo/GO_0016310 | denotes | phosphorylation |
| T510 | 621-636 | http://purl.obolibrary.org/obo/GO_0016310 | denotes | phosphorylation |
| T514 | 543-558 | http://purl.obolibrary.org/obo/GO_0006351 | denotes | transcriptional |
| T515 | 122-125 | http://purl.obolibrary.org/obo/GO_0004697 | denotes | PKC |
| T516 | 166-170 | http://purl.obolibrary.org/obo/GO_0004697 | denotes | PKCα |
| T517 | 228-232 | http://purl.obolibrary.org/obo/GO_0004697 | denotes | PKCα |
| T518 | 248-252 | http://purl.obolibrary.org/obo/GO_0004697 | denotes | PKCα |
| T519 | 484-488 | http://purl.obolibrary.org/obo/GO_0004697 | denotes | PKCα |
| T520 | 273-293 | http://purl.obolibrary.org/obo/GO_0014823 | denotes | activity in response |
| T521 | 285-302 | http://purl.obolibrary.org/obo/GO_1903969 | denotes | response to M-CSF |
| T522 | 285-302 | http://purl.obolibrary.org/obo/GO_0036005 | denotes | response to M-CSF |
| T523 | 285-302 | http://purl.obolibrary.org/obo/GO_1903970 | denotes | response to M-CSF |
| T524 | 285-302 | http://purl.obolibrary.org/obo/GO_1903971 | denotes | response to M-CSF |
| T525 | 559-576 | http://purl.obolibrary.org/obo/GO_0051092 | denotes | activation, NF-κB |
| T526 | 755-771 | http://purl.obolibrary.org/obo/GO_0051092 | denotes | NF-κB activation |
| T527 | 587-602 | http://purl.obolibrary.org/obo/GO_0010467 | denotes | gene expression |
| T4585 | 948-957 | http://purl.obolibrary.org/obo/GO_0006915 | denotes | apoptosis |
| T4639 | 948-957 | http://purl.obolibrary.org/obo/GO_0097194 | denotes | apoptosis |
UBERON-AE
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T960 | 849-860 | http://purl.obolibrary.org/obo/UBERON_0002371 | denotes | bone marrow |
| T961 | 878-883 | http://purl.obolibrary.org/obo/UBERON_0000178 | denotes | blood |
| T962 | 923-928 | http://purl.obolibrary.org/obo/UBERON_0001977 | denotes | serum |
| T963 | 1246-1252 | http://purl.obolibrary.org/obo/UBERON_0000310 | denotes | breast |
pmc-enju-pas
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T322 | 0-2 | IN | denotes | in |
| T323 | 3-8 | JJ | denotes | human |
| T324 | 9-25 | JJ | denotes | monocyte-derived |
| T325 | 26-37 | NN | denotes | macrophages |
| T326 | 38-39 | -LRB- | denotes | ( |
| T327 | 39-43 | NN | denotes | MDMs |
| T328 | 43-44 | -RRB- | denotes | ) |
| T329 | 45-48 | CC | denotes | and |
| T330 | 49-52 | DT | denotes | the |
| T331 | 53-59 | JJ | denotes | murine |
| T332 | 60-70 | NN | denotes | macrophage |
| T333 | 71-75 | NN | denotes | cell |
| T334 | 76-80 | NN | denotes | line |
| T335 | 81-84 | NN | denotes | RAW |
| T336 | 85-90 | CD | denotes | 264.7 |
| T337 | 92-95 | DT | denotes | The |
| T338 | 96-103 | JJ | denotes | general |
| T339 | 104-111 | NN | denotes | protein |
| T340 | 112-118 | NN | denotes | kinase |
| T341 | 119-120 | NN | denotes | C |
| T342 | 121-122 | -LRB- | denotes | ( |
| T343 | 122-125 | NN | denotes | PKC |
| T344 | 125-126 | -RRB- | denotes | ) |
| T345 | 127-136 | NN | denotes | inhibitor |
| T346 | 137-147 | NN | denotes | Ro-31-8220 |
| T347 | 147-148 | -COMMA- | denotes | , |
| T348 | 149-152 | DT | denotes | the |
| T349 | 153-165 | JJ | denotes | conventional |
| T350 | 166-172 | NN | denotes | PKCα/β |
| T351 | 173-182 | NN | denotes | inhibitor |
| T352 | 183-190 | NN | denotes | Gö-6976 |
| T353 | 190-191 | -COMMA- | denotes | , |
| T354 | 192-206 | NN | denotes | overexpression |
| T355 | 207-209 | IN | denotes | of |
| T356 | 210-218 | JJ | denotes | dominant |
| T357 | 219-227 | JJ | denotes | negative |
| T358 | 228-232 | NN | denotes | PKCα |
| T359 | 233-243 | NN | denotes | constructs |
| T360 | 244-247 | CC | denotes | and |
| T361 | 248-252 | NN | denotes | PKCα |
| T362 | 253-258 | NN | denotes | siRNA |
| T363 | 259-266 | VB | denotes | reduced |
| T364 | 267-272 | NN | denotes | NF-κB |
| T365 | 273-281 | NN | denotes | activity |
| T366 | 282-284 | IN | denotes | in |
| T367 | 285-293 | NN | denotes | response |
| T368 | 294-296 | TO | denotes | to |
| T369 | 297-302 | NN | denotes | M-CSF |
| T370 | 304-317 | RB | denotes | Interestingly |
| T371 | 317-318 | -COMMA- | denotes | , |
| T372 | 319-329 | NN | denotes | Ro-31-8220 |
| T373 | 330-337 | VB | denotes | reduced |
| T374 | 338-344 | NN | denotes | Ser276 |
| T375 | 345-360 | NN | denotes | phosphorylation |
| T376 | 361-363 | IN | denotes | of |
| T377 | 364-372 | NN | denotes | NF-κBp65 |
| T378 | 373-380 | VB | denotes | leading |
| T379 | 381-383 | TO | denotes | to |
| T380 | 384-393 | VB | denotes | decreased |
| T381 | 394-407 | JJ | denotes | M-CSF-induced |
| T382 | 408-416 | NN | denotes | monocyte |
| T383 | 417-425 | NN | denotes | survival |
| T384 | 427-429 | IN | denotes | In |
| T385 | 430-434 | DT | denotes | this |
| T386 | 435-441 | NN | denotes | report |
| T387 | 441-442 | -COMMA- | denotes | , |
| T388 | 443-445 | PRP | denotes | we |
| T389 | 446-454 | VB | denotes | identify |
| T390 | 455-467 | JJ | denotes | conventional |
| T391 | 468-472 | NN | denotes | PKCs |
| T392 | 472-473 | -COMMA- | denotes | , |
| T393 | 474-483 | VB | denotes | including |
| T394 | 484-488 | NN | denotes | PKCα |
| T395 | 489-491 | IN | denotes | as |
| T396 | 492-501 | JJ | denotes | important |
| T397 | 502-510 | JJ | denotes | upstream |
| T398 | 511-518 | NN | denotes | kinases |
| T399 | 519-522 | IN | denotes | for |
| T400 | 523-536 | JJ | denotes | M-CSF-induced |
| T401 | 537-542 | NN | denotes | NF-κB |
| T402 | 543-558 | JJ | denotes | transcriptional |
| T403 | 559-569 | NN | denotes | activation |
| T404 | 569-570 | -COMMA- | denotes | , |
| T405 | 571-586 | JJ | denotes | NF-κB-regulated |
| T406 | 587-591 | NN | denotes | gene |
| T407 | 592-602 | NN | denotes | expression |
| T408 | 602-603 | -COMMA- | denotes | , |
| T409 | 604-609 | NN | denotes | NF-κB |
| T410 | 610-613 | NN | denotes | p65 |
| T411 | 614-620 | NN | denotes | Ser276 |
| T412 | 621-636 | NN | denotes | phosphorylation |
| T413 | 636-637 | -COMMA- | denotes | , |
| T414 | 638-641 | CC | denotes | and |
| T415 | 642-652 | NN | denotes | macrophage |
| T416 | 653-661 | NN | denotes | survival |
| T417 | 663-669 | RB | denotes | Lastly |
| T418 | 669-670 | -COMMA- | denotes | , |
| T419 | 671-673 | PRP | denotes | we |
| T420 | 674-678 | VB | denotes | find |
| T421 | 679-683 | IN | denotes | that |
| T422 | 684-689 | NN | denotes | NF-κB |
| T423 | 690-693 | NN | denotes | p65 |
| T424 | 694-700 | NN | denotes | Ser276 |
| T425 | 701-706 | VB | denotes | plays |
| T426 | 707-709 | DT | denotes | an |
| T427 | 710-719 | JJ | denotes | important |
| T428 | 720-724 | NN | denotes | role |
| T429 | 725-727 | IN | denotes | in |
| T430 | 728-733 | JJ | denotes | basal |
| T431 | 734-737 | CC | denotes | and |
| T432 | 738-754 | JJ | denotes | M-CSF-stimulated |
| T433 | 755-760 | NN | denotes | NF-κB |
| T434 | 761-771 | NN | denotes | activation |
| T435 | 772-774 | IN | denotes | in |
| T436 | 775-780 | JJ | denotes | human |
| T437 | 781-792 | JJ | denotes | mononuclear |
| T438 | 793-803 | NN | denotes | phagocytes |
| T2187 | 819-828 | NN | denotes | Monocytes |
| T2188 | 829-832 | VB | denotes | are |
| T2189 | 833-841 | VB | denotes | produced |
| T2190 | 842-844 | IN | denotes | in |
| T2191 | 845-848 | DT | denotes | the |
| T2192 | 849-853 | NN | denotes | bone |
| T2193 | 854-860 | NN | denotes | marrow |
| T2194 | 861-864 | CC | denotes | and |
| T2195 | 865-874 | VB | denotes | circulate |
| T2196 | 875-877 | IN | denotes | in |
| T2197 | 878-883 | NN | denotes | blood |
| T2198 | 884-887 | IN | denotes | for |
| T2199 | 888-893 | CD | denotes | 24–48 |
| T2200 | 894-899 | NN | denotes | hours |
| T2201 | 900-901 | -LRB- | denotes | [ |
| T2202 | 901-902 | CD | denotes | 1 |
| T2203 | 902-903 | -RRB- | denotes | ] |
| T2204 | 905-907 | IN | denotes | In |
| T2205 | 908-911 | DT | denotes | the |
| T2206 | 912-919 | NN | denotes | absence |
| T2207 | 920-922 | IN | denotes | of |
| T2208 | 923-928 | NN | denotes | serum |
| T2209 | 928-929 | -COMMA- | denotes | , |
| T2210 | 930-939 | NN | denotes | monocytes |
| T2211 | 940-943 | VB | denotes | die |
| T2212 | 944-947 | IN | denotes | via |
| T2213 | 948-957 | NN | denotes | apoptosis |
| T2214 | 958-959 | -LRB- | denotes | [ |
| T2215 | 959-960 | CD | denotes | 1 |
| T2216 | 960-961 | -RRB- | denotes | ] |
| T2217 | 961-962 | -COMMA- | denotes | , |
| T2218 | 963-964 | -LRB- | denotes | [ |
| T2219 | 964-965 | CD | denotes | 2 |
| T2220 | 965-966 | -RRB- | denotes | ] |
| T2221 | 968-978 | NN | denotes | Macrophage |
| T2222 | 979-997 | JJ | denotes | colony-stimulating |
| T2223 | 998-1004 | NN | denotes | factor |
| T2224 | 1005-1006 | -LRB- | denotes | ( |
| T2225 | 1006-1011 | NN | denotes | M-CSF |
| T2226 | 1011-1012 | -RRB- | denotes | ) |
| T2227 | 1013-1023 | VB | denotes | stimulates |
| T2228 | 1024-1035 | JJ | denotes | mononuclear |
| T2229 | 1036-1045 | NN | denotes | phagocyte |
| T2230 | 1046-1054 | NN | denotes | survival |
| T2231 | 1055-1058 | CC | denotes | and |
| T2232 | 1059-1074 | NN | denotes | differentiation |
| T2233 | 1075-1076 | -LRB- | denotes | [ |
| T2234 | 1076-1077 | CD | denotes | 3 |
| T2235 | 1077-1078 | -RRB- | denotes | ] |
| T2236 | 1080-1091 | RB | denotes | Importantly |
| T2237 | 1091-1092 | -COMMA- | denotes | , |
| T2238 | 1093-1107 | JJ | denotes | M-CSF-mediated |
| T2239 | 1108-1112 | NN | denotes | cell |
| T2240 | 1113-1121 | NN | denotes | survival |
| T2241 | 1122-1125 | CC | denotes | and |
| T2242 | 1126-1136 | NN | denotes | activation |
| T2243 | 1137-1139 | VB | denotes | is |
| T2244 | 1140-1150 | VB | denotes | associated |
| T2245 | 1151-1155 | IN | denotes | with |
| T2246 | 1156-1157 | DT | denotes | a |
| T2247 | 1158-1165 | NN | denotes | variety |
| T2248 | 1166-1168 | IN | denotes | of |
| T2249 | 1169-1174 | JJ | denotes | human |
| T2250 | 1175-1183 | NN | denotes | diseases |
| T2251 | 1183-1184 | -COMMA- | denotes | , |
| T2252 | 1185-1194 | VB | denotes | including |
| T2253 | 1195-1210 | NN | denotes | atherosclerosis |
| T2254 | 1210-1211 | -COMMA- | denotes | , |
| T2255 | 1212-1222 | NN | denotes | transplant |
| T2256 | 1223-1231 | JJ | denotes | vascular |
| T2257 | 1232-1241 | NN | denotes | sclerosis |
| T2258 | 1242-1245 | CC | denotes | and |
| T2259 | 1246-1252 | NN | denotes | breast |
| T2260 | 1253-1259 | NN | denotes | cancer |
| T2261 | 1260-1270 | NN | denotes | metastasis |
| T2262 | 1271-1272 | -LRB- | denotes | [ |
| T2263 | 1272-1273 | CD | denotes | 4 |
| T2264 | 1273-1274 | -RRB- | denotes | ] |
| T2265 | 1274-1275 | -COMMA- | denotes | , |
| T2266 | 1276-1277 | -LRB- | denotes | [ |
| T2267 | 1277-1278 | CD | denotes | 5 |
| T2268 | 1278-1279 | -RRB- | denotes | ] |
| T2269 | 1279-1280 | -COMMA- | denotes | , |
| T2270 | 1281-1282 | -LRB- | denotes | [ |
| T2271 | 1282-1283 | CD | denotes | 6 |
| T2272 | 1283-1284 | -RRB- | denotes | ] |
| T2273 | 1286-1288 | PRP | denotes | We |
| T2274 | 1289-1299 | RB | denotes | previously |
| T2275 | 1300-1310 | VB | denotes | identified |
| T2276 | 1311-1315 | IN | denotes | that |
| T2277 | 1316-1321 | NN | denotes | NF-κB |
| T2278 | 1322-1332 | NN | denotes | activation |
| T2279 | 1333-1335 | VB | denotes | is |
| T15743 | 0-1335 | VB | denotes | in human monocyte-derived macrophages (MDMs) and the murine macrophage cell line RAW 264.7. The general protein kinase C (PKC) inhibitor Ro-31-8220, the conventional PKCα/β inhibitor Gö-6976, overexpression of dominant negative PKCα constructs and PKCα siRNA reduced NF-κB activity in response to M-CSF. Interestingly, Ro-31-8220 reduced Ser276 phosphorylation of NF-κBp65 leading to decreased M-CSF-induced monocyte survival. In this report, we identify conventional PKCs, including PKCα as important upstream kinases for M-CSF-induced NF-κB transcriptional activation, NF-κB-regulated gene expression, NF-κB p65 Ser276 phosphorylation, and macrophage survival. Lastly, we find that NF-κB p65 Ser276 plays an important role in basal and M-CSF-stimulated NF-κB activation in human mononuclear phagocytes. Introduction Monocytes are produced in the bone marrow and circulate in blood for 24–48 hours [1]. In the absence of serum, monocytes die via apoptosis [1], [2]. Macrophage colony-stimulating factor (M-CSF) stimulates mononuclear phagocyte survival and differentiation [3]. Importantly, M-CSF-mediated cell survival and activation is associated with a variety of human diseases, including atherosclerosis, transplant vascular sclerosis and breast cancer metastasis [4], [5], [6]. We previously identified that NF-κB activation is |
| R288 | T325 | T322 | arg2Of | macrophages,in |
| R289 | T325 | T323 | arg1Of | macrophages,human |
| R290 | T325 | T324 | arg1Of | macrophages,monocyte-derived |
| R291 | T325 | T326 | arg1Of | macrophages,( |
| R292 | T327 | T326 | arg2Of | MDMs,( |
| R293 | T328 | T326 | arg3Of | ),( |
| R295 | T335 | T329 | arg2Of | RAW,and |
| R296 | T335 | T330 | arg1Of | RAW,the |
| R297 | T335 | T331 | arg1Of | RAW,murine |
| R298 | T335 | T332 | arg1Of | RAW,macrophage |
| R299 | T335 | T333 | arg1Of | RAW,cell |
| R300 | T335 | T334 | arg1Of | RAW,line |
| R301 | T335 | T336 | arg1Of | RAW,264.7 |
| R302 | T341 | T338 | arg1Of | C,general |
| R303 | T341 | T339 | arg1Of | C,protein |
| R304 | T341 | T340 | arg1Of | C,kinase |
| R305 | T341 | T342 | arg1Of | C,( |
| R306 | T343 | T342 | arg2Of | PKC,( |
| R307 | T344 | T342 | arg3Of | ),( |
| R308 | T346 | T337 | arg1Of | Ro-31-8220,The |
| R309 | T346 | T341 | arg1Of | Ro-31-8220,C |
| R310 | T346 | T345 | arg1Of | Ro-31-8220,inhibitor |
| R311 | T346 | T347 | arg1Of | Ro-31-8220,"," |
| R312 | T346 | T353 | arg1Of | Ro-31-8220,"," |
| R313 | T352 | T347 | arg2Of | Gö-6976,"," |
| R314 | T352 | T348 | arg1Of | Gö-6976,the |
| R315 | T352 | T349 | arg1Of | Gö-6976,conventional |
| R316 | T352 | T350 | arg1Of | Gö-6976,PKCα/β |
| R317 | T352 | T351 | arg1Of | Gö-6976,inhibitor |
| R318 | T353 | T360 | arg1Of | ",",and |
| R319 | T354 | T353 | arg2Of | overexpression,"," |
| R320 | T354 | T355 | arg1Of | overexpression,of |
| R321 | T359 | T355 | arg2Of | constructs,of |
| R322 | T359 | T356 | arg1Of | constructs,dominant |
| R323 | T359 | T357 | arg1Of | constructs,negative |
| R324 | T359 | T358 | arg1Of | constructs,PKCα |
| R325 | T360 | T363 | arg1Of | and,reduced |
| R326 | T362 | T360 | arg2Of | siRNA,and |
| R327 | T362 | T361 | arg1Of | siRNA,PKCα |
| R328 | T363 | T366 | arg1Of | reduced,in |
| R329 | T365 | T363 | arg2Of | activity,reduced |
| R330 | T365 | T364 | arg1Of | activity,NF-κB |
| R331 | T367 | T366 | arg2Of | response,in |
| R332 | T367 | T368 | arg1Of | response,to |
| R333 | T369 | T368 | arg2Of | M-CSF,to |
| R334 | T372 | T373 | arg1Of | Ro-31-8220,reduced |
| R335 | T373 | T370 | arg1Of | reduced,Interestingly |
| R336 | T373 | T371 | arg1Of | reduced,"," |
| R337 | T375 | T373 | arg2Of | phosphorylation,reduced |
| R338 | T375 | T374 | arg1Of | phosphorylation,Ser276 |
| R339 | T375 | T376 | arg1Of | phosphorylation,of |
| R340 | T375 | T378 | arg1Of | phosphorylation,leading |
| R341 | T377 | T376 | arg2Of | NF-κBp65,of |
| R342 | T378 | T379 | arg1Of | leading,to |
| R343 | T383 | T379 | arg2Of | survival,to |
| R344 | T383 | T380 | arg2Of | survival,decreased |
| R345 | T383 | T381 | arg1Of | survival,M-CSF-induced |
| R346 | T383 | T382 | arg1Of | survival,monocyte |
| R347 | T386 | T384 | arg2Of | report,In |
| R348 | T386 | T385 | arg1Of | report,this |
| R349 | T388 | T389 | arg1Of | we,identify |
| R350 | T389 | T384 | arg1Of | identify,In |
| R351 | T389 | T387 | arg1Of | identify,"," |
| R352 | T391 | T389 | arg2Of | PKCs,identify |
| R353 | T391 | T390 | arg1Of | PKCs,conventional |
| R354 | T391 | T392 | arg1Of | PKCs,"," |
| R355 | T391 | T393 | arg1Of | PKCs,including |
| R356 | T394 | T393 | arg2Of | PKCα,including |
| R357 | T394 | T395 | arg1Of | PKCα,as |
| R358 | T398 | T396 | arg1Of | kinases,important |
| R359 | T398 | T397 | arg1Of | kinases,upstream |
| R360 | T398 | T399 | arg1Of | kinases,for |
| R361 | T398 | T414 | arg1Of | kinases,and |
| R362 | T403 | T400 | arg1Of | activation,M-CSF-induced |
| R363 | T403 | T401 | arg1Of | activation,NF-κB |
| R364 | T403 | T402 | arg1Of | activation,transcriptional |
| R365 | T403 | T404 | arg1Of | activation,"," |
| R366 | T404 | T408 | arg1Of | ",","," |
| R367 | T407 | T404 | arg2Of | expression,"," |
| R368 | T407 | T405 | arg1Of | expression,NF-κB-regulated |
| R369 | T407 | T406 | arg1Of | expression,gene |
| R370 | T408 | T399 | arg2Of | ",",for |
| R371 | T412 | T408 | arg2Of | phosphorylation,"," |
| R372 | T412 | T409 | arg1Of | phosphorylation,NF-κB |
| R373 | T412 | T410 | arg1Of | phosphorylation,p65 |
| R374 | T412 | T411 | arg1Of | phosphorylation,Ser276 |
| R375 | T414 | T395 | arg2Of | and,as |
| R376 | T414 | T413 | arg1Of | and,"," |
| R377 | T416 | T414 | arg2Of | survival,and |
| R378 | T416 | T415 | arg1Of | survival,macrophage |
| R379 | T419 | T420 | arg1Of | we,find |
| R380 | T420 | T417 | arg1Of | find,Lastly |
| R381 | T420 | T418 | arg1Of | find,"," |
| R382 | T424 | T422 | arg1Of | Ser276,NF-κB |
| R383 | T424 | T423 | arg1Of | Ser276,p65 |
| R384 | T424 | T425 | arg1Of | Ser276,plays |
| R385 | T425 | T420 | arg2Of | plays,find |
| R386 | T425 | T421 | arg1Of | plays,that |
| R387 | T425 | T429 | arg1Of | plays,in |
| R388 | T428 | T425 | arg2Of | role,plays |
| R389 | T428 | T426 | arg1Of | role,an |
| R390 | T428 | T427 | arg1Of | role,important |
| R391 | T430 | T431 | arg1Of | basal,and |
| R392 | T432 | T431 | arg2Of | M-CSF-stimulated,and |
| R393 | T434 | T429 | arg2Of | activation,in |
| R394 | T434 | T430 | arg1Of | activation,basal |
| R395 | T434 | T432 | arg1Of | activation,M-CSF-stimulated |
| R396 | T434 | T433 | arg1Of | activation,NF-κB |
| R397 | T434 | T435 | arg1Of | activation,in |
| R398 | T438 | T435 | arg2Of | phagocytes,in |
| R399 | T438 | T436 | arg1Of | phagocytes,human |
| R400 | T438 | T437 | arg1Of | phagocytes,mononuclear |
| R1754 | T2187 | T2188 | arg1Of | Monocytes,are |
| R1755 | T2187 | T2189 | arg2Of | Monocytes,produced |
| R1756 | T2187 | T2195 | arg1Of | Monocytes,circulate |
| R1757 | T2189 | T2188 | arg2Of | produced,are |
| R1758 | T2189 | T2190 | arg1Of | produced,in |
| R1759 | T2189 | T2194 | arg1Of | produced,and |
| R1762 | T2193 | T2190 | arg2Of | marrow,in |
| R1763 | T2193 | T2191 | arg1Of | marrow,the |
| R1764 | T2193 | T2192 | arg1Of | marrow,bone |
| R1765 | T2195 | T2194 | arg2Of | circulate,and |
| R1766 | T2195 | T2196 | arg1Of | circulate,in |
| R1768 | T2197 | T2196 | arg2Of | blood,in |
| R1769 | T2197 | T2198 | arg1Of | blood,for |
| R1771 | T2200 | T2198 | arg2Of | hours,for |
| R1772 | T2200 | T2199 | arg1Of | hours,24–48 |
| R1773 | T2200 | T2201 | arg1Of | hours,[ |
| R1774 | T2202 | T2201 | arg2Of | 1,[ |
| R1775 | T2203 | T2201 | arg3Of | ],[ |
| R1777 | T2206 | T2204 | arg2Of | absence,In |
| R1778 | T2206 | T2205 | arg1Of | absence,the |
| R1779 | T2206 | T2207 | arg1Of | absence,of |
| R1781 | T2208 | T2207 | arg2Of | serum,of |
| R1783 | T2210 | T2211 | arg1Of | monocytes,die |
| R1784 | T2211 | T2204 | arg1Of | die,In |
| R1785 | T2211 | T2209 | arg1Of | die,"," |
| R1786 | T2211 | T2212 | arg1Of | die,via |
| R1787 | T2211 | T2214 | arg1Of | die,[ |
| R1788 | T2211 | T2217 | arg1Of | die,"," |
| R1789 | T2211 | T2218 | arg1Of | die,[ |
| R1790 | T2213 | T2212 | arg2Of | apoptosis,via |
| R1792 | T2215 | T2214 | arg2Of | 1,[ |
| R1793 | T2216 | T2214 | arg3Of | ],[ |
| R1796 | T2219 | T2218 | arg2Of | 2,[ |
| R1797 | T2220 | T2218 | arg3Of | ],[ |
| R1799 | T2223 | T2221 | arg1Of | factor,Macrophage |
| R1800 | T2223 | T2222 | arg1Of | factor,colony-stimulating |
| R1801 | T2223 | T2224 | arg1Of | factor,( |
| R1802 | T2223 | T2227 | arg1Of | factor,stimulates |
| R1803 | T2225 | T2224 | arg2Of | M-CSF,( |
| R1805 | T2226 | T2224 | arg3Of | ),( |
| R1807 | T2230 | T2228 | arg1Of | survival,mononuclear |
| R1808 | T2230 | T2229 | arg1Of | survival,phagocyte |
| R1809 | T2230 | T2231 | arg1Of | survival,and |
| R1811 | T2231 | T2227 | arg2Of | and,stimulates |
| R1812 | T2232 | T2231 | arg2Of | differentiation,and |
| R1813 | T2232 | T2233 | arg1Of | differentiation,[ |
| R1814 | T2234 | T2233 | arg2Of | 3,[ |
| R1816 | T2235 | T2233 | arg3Of | ],[ |
| R1819 | T2240 | T2238 | arg1Of | survival,M-CSF-mediated |
| R1820 | T2240 | T2239 | arg1Of | survival,cell |
| R1821 | T2240 | T2241 | arg1Of | survival,and |
| R1822 | T2241 | T2243 | arg1Of | and,is |
| R1823 | T2241 | T2244 | arg2Of | and,associated |
| R1825 | T2242 | T2241 | arg2Of | activation,and |
| R1828 | T2244 | T2236 | arg1Of | associated,Importantly |
| R1829 | T2244 | T2237 | arg1Of | associated,"," |
| R1830 | T2244 | T2243 | arg2Of | associated,is |
| R1831 | T2244 | T2245 | arg1Of | associated,with |
| R1832 | T2244 | T2265 | arg1Of | associated,"," |
| R1833 | T2244 | T2266 | arg1Of | associated,[ |
| R1834 | T2244 | T2269 | arg1Of | associated,"," |
| R1835 | T2244 | T2270 | arg1Of | associated,[ |
| R1837 | T2247 | T2245 | arg2Of | variety,with |
| R1838 | T2247 | T2246 | arg1Of | variety,a |
| R1839 | T2247 | T2248 | arg1Of | variety,of |
| R1841 | T2250 | T2248 | arg2Of | diseases,of |
| R1842 | T2250 | T2249 | arg1Of | diseases,human |
| R1843 | T2250 | T2251 | arg1Of | diseases,"," |
| R1844 | T2250 | T2252 | arg1Of | diseases,including |
| R1846 | T2253 | T2254 | arg1Of | atherosclerosis,"," |
| R1847 | T2254 | T2258 | arg1Of | ",",and |
| R1849 | T2257 | T2254 | arg2Of | sclerosis,"," |
| R1850 | T2257 | T2255 | arg1Of | sclerosis,transplant |
| R1851 | T2257 | T2256 | arg1Of | sclerosis,vascular |
| R1852 | T2258 | T2252 | arg2Of | and,including |
| R1854 | T2261 | T2258 | arg2Of | metastasis,and |
| R1855 | T2261 | T2259 | arg1Of | metastasis,breast |
| R1856 | T2261 | T2260 | arg1Of | metastasis,cancer |
| R1857 | T2261 | T2262 | arg1Of | metastasis,[ |
| R1858 | T2263 | T2262 | arg2Of | 4,[ |
| R1860 | T2264 | T2262 | arg3Of | ],[ |
| R1862 | T2267 | T2266 | arg2Of | 5,[ |
| R1863 | T2268 | T2266 | arg3Of | ],[ |
| R1866 | T2271 | T2270 | arg2Of | 6,[ |
| R1867 | T2272 | T2270 | arg3Of | ],[ |
| R1869 | T2273 | T2275 | arg1Of | We,identified |
| R1870 | T2275 | T2274 | arg1Of | identified,previously |
| R1872 | T2278 | T2277 | arg1Of | activation,NF-κB |
| R1873 | T2278 | T2279 | arg1Of | activation,is |
| R1875 | T2279 | T2275 | arg2Of | is,identified |
| R1876 | T2279 | T2276 | arg1Of | is,that |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T54 | 92-303 | Sentence | denotes | The general protein kinase C (PKC) inhibitor Ro-31-8220, the conventional PKCα/β inhibitor Gö-6976, overexpression of dominant negative PKCα constructs and PKCα siRNA reduced NF-κB activity in response to M-CSF. |
| T55 | 304-426 | Sentence | denotes | Interestingly, Ro-31-8220 reduced Ser276 phosphorylation of NF-κBp65 leading to decreased M-CSF-induced monocyte survival. |
| T56 | 427-662 | Sentence | denotes | In this report, we identify conventional PKCs, including PKCα as important upstream kinases for M-CSF-induced NF-κB transcriptional activation, NF-κB-regulated gene expression, NF-κB p65 Ser276 phosphorylation, and macrophage survival. |
| T57 | 663-804 | Sentence | denotes | Lastly, we find that NF-κB p65 Ser276 plays an important role in basal and M-CSF-stimulated NF-κB activation in human mononuclear phagocytes. |
| T968 | 819-904 | Sentence | denotes | Monocytes are produced in the bone marrow and circulate in blood for 24–48 hours [1]. |
| T969 | 905-967 | Sentence | denotes | In the absence of serum, monocytes die via apoptosis [1], [2]. |
| T970 | 968-1079 | Sentence | denotes | Macrophage colony-stimulating factor (M-CSF) stimulates mononuclear phagocyte survival and differentiation [3]. |
| T971 | 1080-1285 | Sentence | denotes | Importantly, M-CSF-mediated cell survival and activation is associated with a variety of human diseases, including atherosclerosis, transplant vascular sclerosis and breast cancer metastasis [4], [5], [6]. |
| T6 | 92-303 | Sentence | denotes | The general protein kinase C (PKC) inhibitor Ro-31-8220, the conventional PKCα/β inhibitor Gö-6976, overexpression of dominant negative PKCα constructs and PKCα siRNA reduced NF-κB activity in response to M-CSF. |
| T7 | 304-426 | Sentence | denotes | Interestingly, Ro-31-8220 reduced Ser276 phosphorylation of NF-κBp65 leading to decreased M-CSF-induced monocyte survival. |
| T8 | 427-662 | Sentence | denotes | In this report, we identify conventional PKCs, including PKCα as important upstream kinases for M-CSF-induced NF-κB transcriptional activation, NF-κB-regulated gene expression, NF-κB p65 Ser276 phosphorylation, and macrophage survival. |
| T9 | 663-804 | Sentence | denotes | Lastly, we find that NF-κB p65 Ser276 plays an important role in basal and M-CSF-stimulated NF-κB activation in human mononuclear phagocytes. |
| T10 | 806-818 | Sentence | denotes | Introduction |
| T11 | 819-904 | Sentence | denotes | Monocytes are produced in the bone marrow and circulate in blood for 24–48 hours [1]. |
| T12 | 905-967 | Sentence | denotes | In the absence of serum, monocytes die via apoptosis [1], [2]. |
| T13 | 968-1079 | Sentence | denotes | Macrophage colony-stimulating factor (M-CSF) stimulates mononuclear phagocyte survival and differentiation [3]. |
| T14 | 1080-1285 | Sentence | denotes | Importantly, M-CSF-mediated cell survival and activation is associated with a variety of human diseases, including atherosclerosis, transplant vascular sclerosis and breast cancer metastasis [4], [5], [6]. |
2_test
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| 22216091-355567-90609669 | 901-902 | 355567 | denotes | 1 |
| 22216091-355567-90609670 | 959-960 | 355567 | denotes | 1 |
| 22216091-1940344-90609671 | 964-965 | 1940344 | denotes | 2 |
| 22216091-10473597-90609672 | 1076-1077 | 10473597 | denotes | 3 |
| 22216091-15289345-90609673 | 1272-1273 | 15289345 | denotes | 4 |
| 22216091-8633015-90609674 | 1277-1278 | 8633015 | denotes | 5 |
| 22216091-7667279-90609675 | 1282-1283 | 7667279 | denotes | 6 |
bionlp-st-ge-2016-spacy-parsed
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T129 | 0-2 | IN | denotes | in |
| T130 | 3-8 | JJ | denotes | human |
| T131 | 9-25 | JJ | denotes | monocyte-derived |
| T132 | 26-37 | NNS | denotes | macrophages |
| T133 | 38-39 | -LRB- | denotes | ( |
| T134 | 39-43 | NNS | denotes | MDMs |
| T135 | 43-44 | -RRB- | denotes | ) |
| T136 | 45-48 | CC | denotes | and |
| T137 | 49-52 | DT | denotes | the |
| T138 | 53-59 | NN | denotes | murine |
| T139 | 60-70 | NN | denotes | macrophage |
| T140 | 71-75 | NN | denotes | cell |
| T141 | 76-80 | NN | denotes | line |
| T142 | 81-84 | NNP | denotes | RAW |
| T143 | 85-90 | CD | denotes | 264.7 |
| T144 | 90-91 | . | denotes | . |
| T145 | 92-95 | DT | denotes | The |
| T146 | 96-103 | JJ | denotes | general |
| T147 | 104-111 | NN | denotes | protein |
| T148 | 112-118 | NN | denotes | kinase |
| T149 | 119-120 | NNP | denotes | C |
| T150 | 121-122 | -LRB- | denotes | ( |
| T151 | 122-125 | NNP | denotes | PKC |
| T152 | 125-126 | -RRB- | denotes | ) |
| T153 | 127-136 | NN | denotes | inhibitor |
| T154 | 137-147 | NN | denotes | Ro-31-8220 |
| T155 | 147-148 | , | denotes | , |
| T156 | 149-152 | DT | denotes | the |
| T157 | 153-165 | JJ | denotes | conventional |
| T158 | 166-170 | NNP | denotes | PKCα |
| T159 | 170-171 | NN | denotes | / |
| T160 | 171-172 | NN | denotes | β |
| T161 | 173-182 | NN | denotes | inhibitor |
| T162 | 183-190 | NNP | denotes | Gö-6976 |
| T163 | 190-191 | , | denotes | , |
| T164 | 192-206 | NN | denotes | overexpression |
| T165 | 207-209 | IN | denotes | of |
| T166 | 210-218 | JJ | denotes | dominant |
| T167 | 219-227 | JJ | denotes | negative |
| T168 | 228-232 | NNP | denotes | PKCα |
| T169 | 233-243 | NNS | denotes | constructs |
| T170 | 244-247 | CC | denotes | and |
| T171 | 248-252 | NNP | denotes | PKCα |
| T172 | 253-258 | NNP | denotes | siRNA |
| T173 | 259-266 | VBD | denotes | reduced |
| T174 | 267-272 | JJ | denotes | NF-κB |
| T175 | 273-281 | NN | denotes | activity |
| T176 | 282-284 | IN | denotes | in |
| T177 | 285-293 | NN | denotes | response |
| T178 | 294-296 | TO | denotes | to |
| T179 | 297-302 | NNP | denotes | M-CSF |
| T180 | 302-303 | . | denotes | . |
| T181 | 304-317 | RB | denotes | Interestingly |
| T182 | 317-318 | , | denotes | , |
| T183 | 319-329 | JJ | denotes | Ro-31-8220 |
| T184 | 330-337 | JJ | denotes | reduced |
| T185 | 338-344 | NNP | denotes | Ser276 |
| T186 | 345-360 | NN | denotes | phosphorylation |
| T187 | 361-363 | IN | denotes | of |
| T188 | 364-372 | NN | denotes | NF-κBp65 |
| T189 | 373-380 | VBG | denotes | leading |
| T190 | 381-383 | TO | denotes | to |
| T191 | 384-393 | VBN | denotes | decreased |
| T192 | 394-407 | JJ | denotes | M-CSF-induced |
| T193 | 408-416 | NN | denotes | monocyte |
| T194 | 417-425 | NN | denotes | survival |
| T195 | 425-426 | . | denotes | . |
| T196 | 427-429 | IN | denotes | In |
| T197 | 430-434 | DT | denotes | this |
| T198 | 435-441 | NN | denotes | report |
| T199 | 441-442 | , | denotes | , |
| T200 | 443-445 | PRP | denotes | we |
| T201 | 446-454 | VBP | denotes | identify |
| T202 | 455-467 | JJ | denotes | conventional |
| T203 | 468-472 | NNS | denotes | PKCs |
| T204 | 472-473 | , | denotes | , |
| T205 | 474-483 | VBG | denotes | including |
| T206 | 484-488 | NNP | denotes | PKCα |
| T207 | 489-491 | IN | denotes | as |
| T208 | 492-501 | JJ | denotes | important |
| T209 | 502-510 | JJ | denotes | upstream |
| T210 | 511-518 | NNS | denotes | kinases |
| T211 | 519-522 | IN | denotes | for |
| T212 | 523-536 | JJ | denotes | M-CSF-induced |
| T213 | 537-542 | NN | denotes | NF-κB |
| T214 | 543-558 | JJ | denotes | transcriptional |
| T215 | 559-569 | NN | denotes | activation |
| T216 | 569-570 | , | denotes | , |
| T217 | 571-586 | JJ | denotes | NF-κB-regulated |
| T218 | 587-591 | NN | denotes | gene |
| T219 | 592-602 | NN | denotes | expression |
| T220 | 602-603 | , | denotes | , |
| T221 | 604-609 | NNP | denotes | NF-κB |
| T222 | 610-613 | CD | denotes | p65 |
| T223 | 614-620 | NNP | denotes | Ser276 |
| T224 | 621-636 | NN | denotes | phosphorylation |
| T225 | 636-637 | , | denotes | , |
| T226 | 638-641 | CC | denotes | and |
| T227 | 642-652 | NN | denotes | macrophage |
| T228 | 653-661 | NN | denotes | survival |
| T229 | 661-662 | . | denotes | . |
| T230 | 663-669 | RB | denotes | Lastly |
| T231 | 669-670 | , | denotes | , |
| T232 | 671-673 | PRP | denotes | we |
| T233 | 674-678 | VBP | denotes | find |
| T234 | 679-683 | IN | denotes | that |
| T235 | 684-689 | NNP | denotes | NF-κB |
| T236 | 690-693 | CD | denotes | p65 |
| T237 | 694-700 | NNP | denotes | Ser276 |
| T238 | 701-706 | VBZ | denotes | plays |
| T239 | 707-709 | DT | denotes | an |
| T240 | 710-719 | JJ | denotes | important |
| T241 | 720-724 | NN | denotes | role |
| T242 | 725-727 | IN | denotes | in |
| T243 | 728-733 | JJ | denotes | basal |
| T244 | 734-737 | CC | denotes | and |
| T245 | 738-754 | JJ | denotes | M-CSF-stimulated |
| T246 | 755-760 | NN | denotes | NF-κB |
| T247 | 761-771 | NN | denotes | activation |
| T248 | 772-774 | IN | denotes | in |
| T249 | 775-780 | JJ | denotes | human |
| T250 | 781-792 | NN | denotes | mononuclear |
| T251 | 793-803 | NNS | denotes | phagocytes |
| T252 | 803-804 | . | denotes | . |
| T1018 | 819-828 | NNS | denotes | Monocytes |
| T1019 | 829-832 | VBP | denotes | are |
| T1020 | 833-841 | VBN | denotes | produced |
| T1021 | 842-844 | IN | denotes | in |
| T1022 | 845-848 | DT | denotes | the |
| T1023 | 849-853 | NN | denotes | bone |
| T1024 | 854-860 | NN | denotes | marrow |
| T1025 | 861-864 | CC | denotes | and |
| T1026 | 865-874 | VBP | denotes | circulate |
| T1027 | 875-877 | IN | denotes | in |
| T1028 | 878-883 | NN | denotes | blood |
| T1029 | 884-887 | IN | denotes | for |
| T1030 | 888-890 | CD | denotes | 24 |
| T1031 | 891-893 | CD | denotes | 48 |
| T1032 | 894-899 | NNS | denotes | hours |
| T1033 | 900-901 | NNP | denotes | [ |
| T1034 | 901-902 | CD | denotes | 1 |
| T1035 | 902-903 | NNP | denotes | ] |
| T1036 | 903-904 | . | denotes | . |
| T1037 | 905-907 | IN | denotes | In |
| T1038 | 908-911 | DT | denotes | the |
| T1039 | 912-919 | NN | denotes | absence |
| T1040 | 920-922 | IN | denotes | of |
| T1041 | 923-928 | NN | denotes | serum |
| T1042 | 928-929 | , | denotes | , |
| T1043 | 930-939 | NNS | denotes | monocytes |
| T1044 | 940-943 | VBP | denotes | die |
| T1045 | 944-947 | IN | denotes | via |
| T1046 | 948-957 | NN | denotes | apoptosis |
| T1047 | 958-959 | NN | denotes | [ |
| T1048 | 959-960 | CD | denotes | 1 |
| T1049 | 960-961 | NNP | denotes | ] |
| T1050 | 961-962 | , | denotes | , |
| T1051 | 963-964 | NNP | denotes | [ |
| T1052 | 964-965 | CD | denotes | 2 |
| T1053 | 965-966 | NNP | denotes | ] |
| T1054 | 966-967 | . | denotes | . |
| T1055 | 968-978 | NNP | denotes | Macrophage |
| T1056 | 979-997 | JJ | denotes | colony-stimulating |
| T1057 | 998-1004 | NN | denotes | factor |
| T1058 | 1005-1006 | -LRB- | denotes | ( |
| T1059 | 1006-1011 | NNP | denotes | M-CSF |
| T1060 | 1011-1012 | -RRB- | denotes | ) |
| T1061 | 1013-1023 | VBZ | denotes | stimulates |
| T1062 | 1024-1035 | JJ | denotes | mononuclear |
| T1063 | 1036-1045 | JJ | denotes | phagocyte |
| T1064 | 1046-1054 | NN | denotes | survival |
| T1065 | 1055-1058 | CC | denotes | and |
| T1066 | 1059-1074 | NN | denotes | differentiation |
| T1067 | 1075-1076 | NN | denotes | [ |
| T1068 | 1076-1077 | CD | denotes | 3 |
| T1069 | 1077-1078 | NNP | denotes | ] |
| T1070 | 1078-1079 | . | denotes | . |
| T1071 | 1080-1091 | RB | denotes | Importantly |
| T1072 | 1091-1092 | , | denotes | , |
| T1073 | 1093-1107 | JJ | denotes | M-CSF-mediated |
| T1074 | 1108-1112 | NN | denotes | cell |
| T1075 | 1113-1121 | NN | denotes | survival |
| T1076 | 1122-1125 | CC | denotes | and |
| T1077 | 1126-1136 | NN | denotes | activation |
| T1078 | 1137-1139 | VBZ | denotes | is |
| T1079 | 1140-1150 | VBN | denotes | associated |
| T1080 | 1151-1155 | IN | denotes | with |
| T1081 | 1156-1157 | DT | denotes | a |
| T1082 | 1158-1165 | NN | denotes | variety |
| T1083 | 1166-1168 | IN | denotes | of |
| T1084 | 1169-1174 | JJ | denotes | human |
| T1085 | 1175-1183 | NNS | denotes | diseases |
| T1086 | 1183-1184 | , | denotes | , |
| T1087 | 1185-1194 | VBG | denotes | including |
| T1088 | 1195-1210 | NN | denotes | atherosclerosis |
| T1089 | 1210-1211 | , | denotes | , |
| T1090 | 1212-1222 | NN | denotes | transplant |
| T1091 | 1223-1231 | JJ | denotes | vascular |
| T1092 | 1232-1241 | NN | denotes | sclerosis |
| T1093 | 1242-1245 | CC | denotes | and |
| T1094 | 1246-1252 | NN | denotes | breast |
| T1095 | 1253-1259 | NN | denotes | cancer |
| T1096 | 1260-1270 | NN | denotes | metastasis |
| T1097 | 1271-1272 | NNP | denotes | [ |
| T1098 | 1272-1273 | CD | denotes | 4 |
| T1099 | 1273-1274 | NNP | denotes | ] |
| T1100 | 1274-1275 | , | denotes | , |
| T1101 | 1276-1277 | NNP | denotes | [ |
| T1102 | 1277-1278 | CD | denotes | 5 |
| T1103 | 1278-1279 | NNP | denotes | ] |
| T1104 | 1279-1280 | , | denotes | , |
| T1105 | 1281-1282 | NNP | denotes | [ |
| T1106 | 1282-1283 | CD | denotes | 6 |
| T1107 | 1283-1284 | NNP | denotes | ] |
| T1108 | 1284-1285 | . | denotes | . |
| T1109 | 1286-1288 | PRP | denotes | We |
| T1110 | 1289-1299 | RB | denotes | previously |
| T1111 | 1300-1310 | VBD | denotes | identified |
| T1112 | 1311-1315 | IN | denotes | that |
| T1113 | 1316-1321 | JJ | denotes | NF-κB |
| T1114 | 1322-1332 | NN | denotes | activation |
| T1115 | 1333-1335 | VBZ | denotes | is |
| T14522 | 0-1335 | VBZ | denotes | in human monocyte-derived macrophages (MDMs) and the murine macrophage cell line RAW 264.7. The general protein kinase C (PKC) inhibitor Ro-31-8220, the conventional PKCα/β inhibitor Gö-6976, overexpression of dominant negative PKCα constructs and PKCα siRNA reduced NF-κB activity in response to M-CSF. Interestingly, Ro-31-8220 reduced Ser276 phosphorylation of NF-κBp65 leading to decreased M-CSF-induced monocyte survival. In this report, we identify conventional PKCs, including PKCα as important upstream kinases for M-CSF-induced NF-κB transcriptional activation, NF-κB-regulated gene expression, NF-κB p65 Ser276 phosphorylation, and macrophage survival. Lastly, we find that NF-κB p65 Ser276 plays an important role in basal and M-CSF-stimulated NF-κB activation in human mononuclear phagocytes. Introduction Monocytes are produced in the bone marrow and circulate in blood for 24–48 hours [1]. In the absence of serum, monocytes die via apoptosis [1], [2]. Macrophage colony-stimulating factor (M-CSF) stimulates mononuclear phagocyte survival and differentiation [3]. Importantly, M-CSF-mediated cell survival and activation is associated with a variety of human diseases, including atherosclerosis, transplant vascular sclerosis and breast cancer metastasis [4], [5], [6]. We previously identified that NF-κB activation is |
| R98 | T130 | T132 | amod | human,macrophages |
| R99 | T131 | T132 | amod | monocyte-derived,macrophages |
| R100 | T132 | T129 | pobj | macrophages,in |
| R101 | T133 | T134 | punct | (,MDMs |
| R102 | T134 | T132 | appos | MDMs,macrophages |
| R105 | T137 | T142 | det | the,RAW |
| R106 | T138 | T141 | nmod | murine,line |
| R107 | T139 | T141 | compound | macrophage,line |
| R108 | T140 | T141 | compound | cell,line |
| R109 | T141 | T142 | compound | line,RAW |
| R111 | T143 | T142 | nummod | 264.7,RAW |
| R113 | T145 | T147 | det | The,protein |
| R114 | T146 | T147 | amod | general,protein |
| R115 | T147 | T173 | nsubj | protein,reduced |
| R116 | T148 | T149 | compound | kinase,C |
| R117 | T149 | T147 | appos | C,protein |
| R118 | T150 | T149 | punct | (,C |
| R119 | T151 | T149 | appos | PKC,C |
| R120 | T152 | T149 | punct | ),C |
| R121 | T153 | T154 | compound | inhibitor,Ro-31-8220 |
| R122 | T154 | T149 | appos | Ro-31-8220,C |
| R123 | T155 | T149 | punct | ",",C |
| R124 | T156 | T162 | det | the,Gö-6976 |
| R125 | T157 | T162 | amod | conventional,Gö-6976 |
| R126 | T158 | T162 | compound | PKCα,Gö-6976 |
| R127 | T159 | T162 | compound | /,Gö-6976 |
| R128 | T160 | T162 | compound | β,Gö-6976 |
| R129 | T161 | T162 | compound | inhibitor,Gö-6976 |
| R130 | T162 | T149 | appos | Gö-6976,C |
| R131 | T163 | T162 | punct | ",",Gö-6976 |
| R132 | T164 | T162 | appos | overexpression,Gö-6976 |
| R133 | T165 | T164 | prep | of,overexpression |
| R134 | T166 | T169 | amod | dominant,constructs |
| R135 | T167 | T169 | amod | negative,constructs |
| R136 | T168 | T169 | compound | PKCα,constructs |
| R137 | T169 | T165 | pobj | constructs,of |
| R138 | T170 | T169 | cc | and,constructs |
| R139 | T171 | T172 | compound | PKCα,siRNA |
| R140 | T172 | T173 | nsubj | siRNA,reduced |
| R141 | T173 | T173 | ROOT | reduced,reduced |
| R142 | T174 | T175 | amod | NF-κB,activity |
| R143 | T175 | T173 | dobj | activity,reduced |
| R144 | T176 | T173 | prep | in,reduced |
| R145 | T177 | T176 | pobj | response,in |
| R146 | T178 | T177 | prep | to,response |
| R147 | T179 | T178 | pobj | M-CSF,to |
| R148 | T180 | T173 | punct | .,reduced |
| R149 | T181 | T184 | advmod | Interestingly,reduced |
| R150 | T182 | T184 | punct | ",",reduced |
| R151 | T183 | T184 | nsubj | Ro-31-8220,reduced |
| R152 | T184 | T184 | ROOT | reduced,reduced |
| R153 | T185 | T186 | compound | Ser276,phosphorylation |
| R154 | T186 | T184 | dobj | phosphorylation,reduced |
| R155 | T187 | T186 | prep | of,phosphorylation |
| R156 | T188 | T189 | compound | NF-κBp65,leading |
| R157 | T189 | T187 | pobj | leading,of |
| R158 | T190 | T189 | prep | to,leading |
| R159 | T191 | T194 | amod | decreased,survival |
| R160 | T192 | T194 | amod | M-CSF-induced,survival |
| R161 | T193 | T194 | compound | monocyte,survival |
| R162 | T194 | T190 | pobj | survival,to |
| R163 | T195 | T184 | punct | .,reduced |
| R164 | T196 | T201 | prep | In,identify |
| R165 | T197 | T198 | det | this,report |
| R166 | T198 | T196 | pobj | report,In |
| R167 | T199 | T201 | punct | ",",identify |
| R168 | T200 | T201 | nsubj | we,identify |
| R169 | T201 | T201 | ROOT | identify,identify |
| R170 | T202 | T203 | amod | conventional,PKCs |
| R171 | T203 | T201 | dobj | PKCs,identify |
| R172 | T204 | T203 | punct | ",",PKCs |
| R173 | T205 | T203 | prep | including,PKCs |
| R174 | T206 | T205 | pobj | PKCα,including |
| R175 | T207 | T201 | prep | as,identify |
| R176 | T208 | T210 | amod | important,kinases |
| R177 | T209 | T210 | compound | upstream,kinases |
| R178 | T210 | T207 | pobj | kinases,as |
| R179 | T211 | T210 | prep | for,kinases |
| R180 | T212 | T215 | amod | M-CSF-induced,activation |
| R181 | T213 | T215 | nmod | NF-κB,activation |
| R182 | T214 | T215 | amod | transcriptional,activation |
| R183 | T215 | T211 | pobj | activation,for |
| R184 | T216 | T215 | punct | ",",activation |
| R185 | T217 | T219 | amod | NF-κB-regulated,expression |
| R186 | T218 | T219 | compound | gene,expression |
| R187 | T219 | T215 | conj | expression,activation |
| R188 | T220 | T215 | punct | ",",activation |
| R189 | T221 | T224 | nmod | NF-κB,phosphorylation |
| R190 | T222 | T224 | nummod | p65,phosphorylation |
| R191 | T223 | T224 | compound | Ser276,phosphorylation |
| R192 | T224 | T215 | conj | phosphorylation,activation |
| R193 | T225 | T224 | punct | ",",phosphorylation |
| R194 | T226 | T224 | cc | and,phosphorylation |
| R195 | T227 | T228 | compound | macrophage,survival |
| R196 | T228 | T224 | conj | survival,phosphorylation |
| R197 | T229 | T201 | punct | .,identify |
| R198 | T230 | T233 | advmod | Lastly,find |
| R199 | T231 | T233 | punct | ",",find |
| R200 | T232 | T233 | nsubj | we,find |
| R201 | T233 | T233 | ROOT | find,find |
| R202 | T234 | T238 | mark | that,plays |
| R203 | T235 | T237 | compound | NF-κB,Ser276 |
| R204 | T236 | T237 | compound | p65,Ser276 |
| R205 | T237 | T238 | nsubj | Ser276,plays |
| R206 | T238 | T233 | ccomp | plays,find |
| R207 | T239 | T241 | det | an,role |
| R208 | T240 | T241 | amod | important,role |
| R209 | T241 | T238 | dobj | role,plays |
| R210 | T242 | T241 | prep | in,role |
| R211 | T243 | T247 | amod | basal,activation |
| R212 | T244 | T243 | cc | and,basal |
| R213 | T245 | T243 | conj | M-CSF-stimulated,basal |
| R214 | T246 | T247 | compound | NF-κB,activation |
| R215 | T247 | T242 | pobj | activation,in |
| R216 | T248 | T247 | prep | in,activation |
| R217 | T249 | T250 | amod | human,mononuclear |
| R218 | T250 | T251 | compound | mononuclear,phagocytes |
| R219 | T251 | T248 | pobj | phagocytes,in |
| R220 | T252 | T233 | punct | .,find |
| R646 | T1018 | T1020 | nsubjpass | Monocytes,produced |
| R647 | T1019 | T1020 | auxpass | are,produced |
| R648 | T1020 | T1020 | ROOT | produced,produced |
| R649 | T1021 | T1020 | prep | in,produced |
| R650 | T1022 | T1024 | det | the,marrow |
| R651 | T1023 | T1024 | compound | bone,marrow |
| R652 | T1024 | T1021 | pobj | marrow,in |
| R653 | T1025 | T1020 | cc | and,produced |
| R654 | T1026 | T1020 | conj | circulate,produced |
| R655 | T1027 | T1026 | prep | in,circulate |
| R656 | T1028 | T1027 | pobj | blood,in |
| R657 | T1029 | T1026 | prep | for,circulate |
| R658 | T1030 | T1029 | pobj | 24,for |
| R659 | T1031 | T1032 | nummod | 48,hours |
| R660 | T1032 | T1030 | appos | hours,24 |
| R661 | T1033 | T1035 | nmod | [,] |
| R662 | T1034 | T1035 | nummod | 1,] |
| R663 | T1035 | T1032 | appos | ],hours |
| R664 | T1036 | T1020 | punct | .,produced |
| R665 | T1037 | T1044 | prep | In,die |
| R666 | T1038 | T1039 | det | the,absence |
| R667 | T1039 | T1037 | pobj | absence,In |
| R668 | T1040 | T1039 | prep | of,absence |
| R669 | T1041 | T1040 | pobj | serum,of |
| R670 | T1042 | T1044 | punct | ",",die |
| R671 | T1043 | T1044 | nsubj | monocytes,die |
| R672 | T1044 | T1044 | ROOT | die,die |
| R673 | T1045 | T1044 | prep | via,die |
| R674 | T1046 | T1047 | compound | apoptosis,[ |
| R675 | T1047 | T1045 | pobj | [,via |
| R676 | T1048 | T1049 | nummod | 1,] |
| R677 | T1049 | T1044 | advcl | ],die |
| R678 | T1050 | T1049 | punct | ",",] |
| R679 | T1051 | T1053 | nmod | [,] |
| R680 | T1052 | T1053 | nummod | 2,] |
| R681 | T1053 | T1049 | appos | ],] |
| R682 | T1054 | T1044 | punct | .,die |
| R683 | T1055 | T1057 | nmod | Macrophage,factor |
| R684 | T1056 | T1057 | amod | colony-stimulating,factor |
| R685 | T1057 | T1061 | nsubj | factor,stimulates |
| R686 | T1058 | T1059 | punct | (,M-CSF |
| R687 | T1059 | T1057 | appos | M-CSF,factor |
| R688 | T1060 | T1057 | punct | ),factor |
| R689 | T1061 | T1061 | ROOT | stimulates,stimulates |
| R690 | T1062 | T1064 | amod | mononuclear,survival |
| R691 | T1063 | T1064 | amod | phagocyte,survival |
| R692 | T1064 | T1069 | nmod | survival,] |
| R693 | T1065 | T1064 | cc | and,survival |
| R694 | T1066 | T1067 | compound | differentiation,[ |
| R695 | T1067 | T1064 | conj | [,survival |
| R696 | T1068 | T1069 | nummod | 3,] |
| R697 | T1069 | T1061 | dobj | ],stimulates |
| R698 | T1070 | T1061 | punct | .,stimulates |
| R699 | T1071 | T1079 | advmod | Importantly,associated |
| R700 | T1072 | T1079 | punct | ",",associated |
| R701 | T1073 | T1075 | amod | M-CSF-mediated,survival |
| R702 | T1074 | T1075 | compound | cell,survival |
| R703 | T1075 | T1079 | nsubjpass | survival,associated |
| R704 | T1076 | T1075 | cc | and,survival |
| R705 | T1077 | T1075 | conj | activation,survival |
| R706 | T1078 | T1079 | auxpass | is,associated |
| R707 | T1079 | T1079 | ROOT | associated,associated |
| R708 | T1080 | T1079 | prep | with,associated |
| R709 | T1081 | T1082 | det | a,variety |
| R710 | T1082 | T1080 | pobj | variety,with |
| R711 | T1083 | T1082 | prep | of,variety |
| R712 | T1084 | T1085 | amod | human,diseases |
| R713 | T1085 | T1083 | pobj | diseases,of |
| R714 | T1086 | T1085 | punct | ",",diseases |
| R715 | T1087 | T1085 | prep | including,diseases |
| R716 | T1088 | T1087 | pobj | atherosclerosis,including |
| R717 | T1089 | T1088 | punct | ",",atherosclerosis |
| R718 | T1090 | T1092 | nmod | transplant,sclerosis |
| R719 | T1091 | T1092 | amod | vascular,sclerosis |
| R720 | T1092 | T1088 | conj | sclerosis,atherosclerosis |
| R721 | T1093 | T1092 | cc | and,sclerosis |
| R722 | T1094 | T1095 | compound | breast,cancer |
| R723 | T1095 | T1096 | compound | cancer,metastasis |
| R724 | T1096 | T1092 | conj | metastasis,sclerosis |
| R725 | T1097 | T1099 | nmod | [,] |
| R726 | T1098 | T1099 | nummod | 4,] |
| R727 | T1099 | T1088 | conj | ],atherosclerosis |
| R728 | T1100 | T1099 | punct | ",",] |
| R729 | T1101 | T1103 | nmod | [,] |
| R730 | T1102 | T1103 | nummod | 5,] |
| R731 | T1103 | T1099 | conj | ],] |
| R732 | T1104 | T1103 | punct | ",",] |
| R733 | T1105 | T1107 | nmod | [,] |
| R734 | T1106 | T1107 | nummod | 6,] |
| R735 | T1107 | T1103 | conj | ],] |
| R736 | T1108 | T1079 | punct | .,associated |
| R737 | T1109 | T1111 | nsubj | We,identified |
| R738 | T1110 | T1111 | advmod | previously,identified |
| R739 | T1111 | T1111 | ROOT | identified,identified |
| R740 | T1112 | T1115 | mark | that,is |
| R741 | T1113 | T1114 | amod | NF-κB,activation |
| R742 | T1114 | T1115 | nsubj | activation,is |
| R743 | T1115 | T1111 | ccomp | is,identified |
| R10605 | T14522 | T14522 | ROOT | "in human monocyte-derived macrophages (MDMs) and the murine macrophage cell line RAW 264.7. The general protein kinase C (PKC) inhibitor Ro-31-8220, the conventional PKCα/β inhibitor Gö-6976, overexpression of dominant negative PKCα constructs and PKCα siRNA reduced NF-κB activity in response to M-CSF. Interestingly, Ro-31-8220 reduced Ser276 phosphorylation of NF-κBp65 leading to decreased M-CSF-induced monocyte survival. In this report, we identify conventional PKCs, including PKCα as important upstream kinases for M-CSF-induced NF-κB transcriptional activation, NF-κB-regulated gene expression, NF-κB p65 Ser276 phosphorylation, and macrophage survival. Lastly, we find that NF-κB p65 Ser276 plays an important role in basal and M-CSF-stimulated NF-κB activation in human mononuclear phagocytes. Introduction Monocytes are produced in the bone marrow and circulate in blood for 24–48 hours [1]. In the absence of serum, monocytes die via apoptosis [1], [2]. Macrophage colony-stimulating factor (M-CSF) stimulates mononuclear phagocyte survival and differentiation [3]. Importantly, M-CSF-mediated cell survival and activation is associated with a variety of human diseases, including atherosclerosis, transplant vascular sclerosis and breast cancer metastasis [4], [5], [6]. We previously identified that NF-κB activation is","in human monocyte-derived macrophages (MDMs) and the murine macrophage cell line RAW 264.7. The general protein kinase C (PKC) inhibitor Ro-31-8220, the conventional PKCα/β inhibitor Gö-6976, overexpression of dominant negative PKCα constructs and PKCα siRNA reduced NF-κB activity in response to M-CSF. Interestingly, Ro-31-8220 reduced Ser276 phosphorylation of NF-κBp65 leading to decreased M-CSF-induced monocyte survival. In this report, we identify conventional PKCs, including PKCα as important upstream kinases for M-CSF-induced NF-κB transcriptional activation, NF-κB-regulated gene expression, NF-κB p65 Ser276 phosphorylation, and macrophage survival. Lastly, we find that NF-κB p65 Ser276 plays an important role in basal and M-CSF-stimulated NF-κB activation in human mononuclear phagocytes. Introduction Monocytes are produced in the bone marrow and circulate in blood for 24–48 hours [1]. In the absence of serum, monocytes die via apoptosis [1], [2]. Macrophage colony-stimulating factor (M-CSF) stimulates mononuclear phagocyte survival and differentiation [3]. Importantly, M-CSF-mediated cell survival and activation is associated with a variety of human diseases, including atherosclerosis, transplant vascular sclerosis and breast cancer metastasis [4], [5], [6]. We previously identified that NF-κB activation is" |
test2
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T31 | 166-170 | Protein | denotes | PKCα |
| T32 | 171-172 | Protein | denotes | β |
| T33 | 173-182 | Negative_regulation | denotes | inhibitor |
| T34 | 210-232 | Protein | denotes | dominant negative PKCα |
| T35 | 248-258 | Protein | denotes | PKCα siRNA |
| T36 | 297-302 | Protein | denotes | M-CSF |
| T37 | 330-337 | Negative_regulation | denotes | reduced |
| T38 | 338-344 | Entity | denotes | Ser276 |
| T39 | 345-360 | Phosphorylation | denotes | phosphorylation |
| T40 | 367-372 | Protein | denotes | κBp65 |
| T41 | 394-399 | Protein | denotes | M-CSF |
| T42 | 484-488 | Protein | denotes | PKCα |
| T43 | 523-528 | Protein | denotes | M-CSF |
| T44 | 610-613 | Protein | denotes | p65 |
| T45 | 614-620 | Entity | denotes | Ser276 |
| T46 | 621-636 | Phosphorylation | denotes | phosphorylation |
| T47 | 690-693 | Protein | denotes | p65 |
| T48 | 694-700 | Entity | denotes | Ser276 |
| T49 | 738-743 | Protein | denotes | M-CSF |
| T907 | 968-1004 | Protein | denotes | Macrophage colony-stimulating factor |
| T908 | 1006-1011 | Protein | denotes | M-CSF |
| T909 | 1093-1098 | Protein | denotes | M-CSF |
| R18 | T31 | T33 | themeOf | PKCα,inhibitor |
| R19 | T32 | T33 | themeOf | β,inhibitor |
| R20 | T38 | T40 | partOf | Ser276,κBp65 |
| R21 | T38 | T39 | themeOf | Ser276,phosphorylation |
| R22 | T39 | T37 | themeOf | phosphorylation,reduced |
| R23 | T45 | T46 | themeOf | Ser276,phosphorylation |
| R24 | T45 | T44 | partOf | Ser276,p65 |
| R25 | T48 | T47 | partOf | Ser276,p65 |
| R616 | T908 | T907 | equivalentTo | M-CSF,Macrophage colony-stimulating factor |