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PMC:3245220 / 48379-49171 JSONTXT

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bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T22664 223-228 P09603 denotes M-CSF
T22665 225-228 P04141 denotes CSF
T22666 677-682 P04406 denotes GAPDH
T22667 723-731 Q04864 denotes relative

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T22668 223-228 Protein denotes M-CSF

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T22686 223-228 Protein denotes M-CSF
T22687 677-682 Protein denotes GAPDH

events-check-again

Id Subject Object Predicate Lexical cue
T22685 223-228 Protein denotes M-CSF

GO-CC

Id Subject Object Predicate Lexical cue
T22677 63-68 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T22678 184-189 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T22679 263-268 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

GO-MF

Id Subject Object Predicate Lexical cue
T22676 489-491 http://purl.obolibrary.org/obo/GO_0033968 denotes CA

GO-BP

Id Subject Object Predicate Lexical cue
T22675 489-491 http://purl.obolibrary.org/obo/GO_0033968 denotes CA

UBERON-AE

Id Subject Object Predicate Lexical cue
T22371 74-79 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T22525 0-3 NN denotes RNA
T22526 4-13 NN denotes Isolation
T22527 14-17 CC denotes and
T22528 18-30 JJ denotes Quantitative
T22529 31-40 JJ denotes Real-time
T22530 41-44 NN denotes PCR
T22531 45-49 NN denotes MDMs
T22532 50-52 CC denotes or
T22533 53-56 NN denotes RAW
T22534 57-62 CD denotes 264.7
T22535 63-68 NN denotes cells
T22536 69-73 VB denotes were
T22537 74-87 JJ denotes serum-starved
T22538 88-97 JJ denotes overnight
T22539 98-100 CC denotes or
T22540 101-104 IN denotes for
T22541 105-106 CD denotes 2
T22542 107-112 NN denotes hours
T22543 112-113 -COMMA- denotes ,
T22544 114-126 RB denotes respectively
T22545 126-127 -COMMA- denotes ,
T22546 128-133 JJ denotes prior
T22547 134-136 TO denotes to
T22548 137-147 VB denotes incubating
T22549 148-152 IN denotes with
T22550 153-163 NN denotes inhibitors
T22551 164-167 IN denotes for
T22552 168-170 CD denotes 30
T22553 171-178 NN denotes minutes
T22554 180-183 DT denotes The
T22555 184-189 NN denotes cells
T22556 190-194 VB denotes were
T22557 195-207 VB denotes restimulated
T22558 208-212 IN denotes with
T22559 213-216 CD denotes 100
T22560 217-222 NN denotes ng/ml
T22561 223-228 NN denotes M-CSF
T22562 229-232 CC denotes and
T22563 233-238 JJ denotes total
T22564 239-243 NN denotes mRNA
T22565 244-247 VB denotes was
T22566 248-257 VB denotes extracted
T22567 258-262 IN denotes from
T22568 263-268 NN denotes cells
T22569 269-273 IN denotes with
T22570 274-280 NN denotes Trizol
T22571 281-282 -LRB- denotes (
T22572 282-292 NN denotes Invitrogen
T22573 292-293 -RRB- denotes )
T22574 294-297 CC denotes and
T22575 298-301 CD denotes 1–2
T22576 302-304 NN denotes µg
T22577 305-307 IN denotes of
T22578 308-313 JJ denotes total
T22579 314-317 NN denotes RNA
T22580 318-321 VB denotes was
T22581 322-326 VB denotes used
T22582 327-329 TO denotes to
T22583 330-341 VB denotes synthesized
T22584 342-346 NN denotes cDNA
T22585 347-352 VB denotes using
T22586 353-364 NN denotes SuperScript
T22587 365-368 CD denotes III
T22588 369-370 -LRB- denotes (
T22589 370-380 NN denotes Invitrogen
T22590 380-381 -RRB- denotes )
T22591 383-395 JJ denotes Quantitative
T22592 396-405 NN denotes real-time
T22593 406-407 -LRB- denotes (
T22594 407-410 NN denotes qRT
T22595 410-411 -RRB- denotes )
T22596 411-415 NN denotes -PCR
T22597 416-419 VB denotes was
T22598 420-429 VB denotes performed
T22599 430-435 VB denotes using
T22600 436-440 NNP denotes SYBR
T22601 441-446 NNP denotes Green
T22602 447-453 NNP denotes Master
T22603 454-457 NNP denotes Mix
T22604 458-459 -LRB- denotes (
T22605 459-466 NNP denotes Applied
T22606 467-477 NNP denotes BioSystems
T22607 477-478 -COMMA- denotes ,
T22608 479-487 NNP denotes Carlsbad
T22609 487-488 -COMMA- denotes ,
T22610 489-491 NNP denotes CA
T22611 491-492 -RRB- denotes )
T22612 494-497 DT denotes The
T22613 498-507 NN denotes reactions
T22614 508-512 VB denotes were
T22615 513-522 VB denotes performed
T22616 523-528 VB denotes using
T22617 529-531 DT denotes an
T22618 532-535 NNP denotes ABI
T22619 536-541 NNP denotes PRIZM
T22620 542-546 CD denotes 7700
T22621 547-554 NN denotes machine
T22622 555-559 IN denotes with
T22623 560-568 NN denotes software
T22624 569-577 NN denotes Sequence
T22625 578-586 NN denotes Detector
T22626 587-594 NN denotes version
T22627 595-598 CD denotes 1.7
T22628 599-600 -LRB- denotes (
T22629 600-607 NNP denotes Applied
T22630 608-618 NNP denotes Biosystems
T22631 618-619 -RRB- denotes )
T22632 621-624 DT denotes The
T22633 625-631 NN denotes target
T22634 632-636 NN denotes gene
T22635 637-643 NN denotes values
T22636 644-648 VB denotes were
T22637 649-659 VB denotes normalized
T22638 660-662 TO denotes to
T22639 663-666 DT denotes the
T22640 667-673 NN denotes values
T22641 674-676 IN denotes of
T22642 677-682 NN denotes GAPDH
T22643 683-685 IN denotes as
T22644 686-687 DT denotes a
T22645 688-700 NN denotes housekeeping
T22646 701-705 NN denotes gene
T22647 706-709 CC denotes and
T22648 710-719 VB denotes expressed
T22649 720-722 IN denotes as
T22650 723-731 JJ denotes relative
T22651 732-736 JJ denotes fold
T22652 737-745 NN denotes increase
T22653 746-747 CD denotes 2
T22654 747-748 -LRB- denotes (
T22655 748-753 NN denotes −ΔΔCt
T22656 753-754 -RRB- denotes )
T22657 755-759 IN denotes over
T22658 760-763 DT denotes the
T22659 764-778 JJ denotes non-stimulated
T22660 779-786 NN denotes samples
T22661 787-788 -LRB- denotes (
T22662 788-790 NN denotes NS
T22663 790-791 -RRB- denotes )
R17115 T22526 T22527 arg1Of Isolation,and
R17116 T22526 T22525 arg1Of Isolation,RNA
R17117 T22530 T22527 arg2Of PCR,and
R17118 T22530 T22528 arg1Of PCR,Quantitative
R17119 T22530 T22529 arg1Of PCR,Real-time
R17120 T22531 T22532 arg1Of MDMs,or
R17121 T22532 T22536 arg1Of or,were
R17122 T22535 T22532 arg2Of cells,or
R17123 T22535 T22533 arg1Of cells,RAW
R17124 T22535 T22534 arg1Of cells,264.7
R17125 T22538 T22537 arg1Of overnight,serum-starved
R17126 T22538 T22539 arg1Of overnight,or
R17127 T22542 T22536 arg2Of hours,were
R17128 T22542 T22538 arg1Of hours,overnight
R17129 T22542 T22540 arg1Of hours,for
R17130 T22542 T22541 arg1Of hours,2
R17131 T22542 T22543 arg1Of hours,","
R17132 T22542 T22544 arg1Of hours,respectively
R17133 T22542 T22545 arg1Of hours,","
R17134 T22542 T22546 arg1Of hours,prior
R17135 T22546 T22547 arg1Of prior,to
R17136 T22548 T22547 arg2Of incubating,to
R17137 T22548 T22549 arg1Of incubating,with
R17138 T22548 T22551 arg1Of incubating,for
R17139 T22550 T22549 arg2Of inhibitors,with
R17140 T22553 T22551 arg2Of minutes,for
R17141 T22553 T22552 arg1Of minutes,30
R17142 T22555 T22554 arg1Of cells,The
R17143 T22555 T22556 arg1Of cells,were
R17144 T22555 T22557 arg2Of cells,restimulated
R17145 T22557 T22556 arg2Of restimulated,were
R17146 T22557 T22558 arg1Of restimulated,with
R17147 T22557 T22562 arg1Of restimulated,and
R17148 T22559 T22560 arg1Of 100,ng/ml
R17149 T22561 T22558 arg2Of M-CSF,with
R17150 T22561 T22559 arg1Of M-CSF,100
R17151 T22562 T22574 arg1Of and,and
R17152 T22564 T22563 arg1Of mRNA,total
R17153 T22564 T22565 arg1Of mRNA,was
R17154 T22564 T22566 arg2Of mRNA,extracted
R17155 T22566 T22562 arg2Of extracted,and
R17156 T22566 T22565 arg2Of extracted,was
R17157 T22566 T22567 arg1Of extracted,from
R17158 T22568 T22567 arg2Of cells,from
R17159 T22568 T22569 arg1Of cells,with
R17160 T22570 T22569 arg2Of Trizol,with
R17161 T22570 T22571 arg1Of Trizol,(
R17162 T22572 T22571 arg2Of Invitrogen,(
R17163 T22573 T22571 arg3Of ),(
R17164 T22576 T22575 arg1Of µg,1–2
R17165 T22576 T22577 arg1Of µg,of
R17166 T22576 T22580 arg1Of µg,was
R17167 T22576 T22581 arg2Of µg,used
R17168 T22579 T22577 arg2Of RNA,of
R17169 T22579 T22578 arg1Of RNA,total
R17170 T22581 T22574 arg2Of used,and
R17171 T22581 T22580 arg2Of used,was
R17172 T22581 T22582 arg1Of used,to
R17173 T22581 T22585 modOf used,using
R17174 T22584 T22582 arg2Of cDNA,to
R17175 T22584 T22583 arg2Of cDNA,synthesized
R17176 T22586 T22585 arg2Of SuperScript,using
R17177 T22586 T22587 arg1Of SuperScript,III
R17178 T22586 T22588 arg1Of SuperScript,(
R17179 T22589 T22588 arg2Of Invitrogen,(
R17180 T22590 T22588 arg3Of ),(
R17181 T22594 T22593 arg2Of qRT,(
R17182 T22595 T22593 arg3Of ),(
R17183 T22596 T22591 arg1Of -PCR,Quantitative
R17184 T22596 T22592 arg1Of -PCR,real-time
R17185 T22596 T22593 arg1Of -PCR,(
R17186 T22596 T22597 arg1Of -PCR,was
R17187 T22596 T22598 arg2Of -PCR,performed
R17188 T22598 T22597 arg2Of performed,was
R17189 T22599 T22598 arg3Of using,performed
R17190 T22603 T22599 arg2Of Mix,using
R17191 T22603 T22600 arg1Of Mix,SYBR
R17192 T22603 T22601 arg1Of Mix,Green
R17193 T22603 T22602 arg1Of Mix,Master
R17194 T22603 T22604 arg1Of Mix,(
R17195 T22606 T22604 arg2Of BioSystems,(
R17196 T22606 T22605 arg1Of BioSystems,Applied
R17197 T22606 T22607 arg1Of BioSystems,","
R17198 T22608 T22607 arg2Of Carlsbad,","
R17199 T22608 T22609 arg1Of Carlsbad,","
R17200 T22610 T22609 arg2Of CA,","
R17201 T22611 T22604 arg3Of ),(
R17202 T22613 T22612 arg1Of reactions,The
R17203 T22613 T22614 arg1Of reactions,were
R17204 T22613 T22615 arg2Of reactions,performed
R17205 T22615 T22614 arg2Of performed,were
R17206 T22616 T22615 arg3Of using,performed
R17207 T22616 T22622 arg1Of using,with
R17208 T22619 T22618 arg1Of PRIZM,ABI
R17209 T22619 T22620 arg1Of PRIZM,7700
R17210 T22621 T22616 arg2Of machine,using
R17211 T22621 T22617 arg1Of machine,an
R17212 T22621 T22619 arg1Of machine,PRIZM
R17213 T22626 T22622 arg2Of version,with
R17214 T22626 T22623 arg1Of version,software
R17215 T22626 T22624 arg1Of version,Sequence
R17216 T22626 T22625 arg1Of version,Detector
R17217 T22626 T22627 arg1Of version,1.7
R17218 T22626 T22628 arg1Of version,(
R17219 T22630 T22628 arg2Of Biosystems,(
R17220 T22630 T22629 arg1Of Biosystems,Applied
R17221 T22631 T22628 arg3Of ),(
R17222 T22635 T22632 arg1Of values,The
R17223 T22635 T22633 arg1Of values,target
R17224 T22635 T22634 arg1Of values,gene
R17225 T22635 T22636 arg1Of values,were
R17226 T22635 T22637 arg2Of values,normalized
R17227 T22635 T22648 arg2Of values,expressed
R17228 T22637 T22638 arg1Of normalized,to
R17229 T22637 T22647 arg1Of normalized,and
R17230 T22640 T22638 arg2Of values,to
R17231 T22640 T22639 arg1Of values,the
R17232 T22640 T22641 arg1Of values,of
R17233 T22640 T22643 arg1Of values,as
R17234 T22642 T22641 arg2Of GAPDH,of
R17235 T22646 T22643 arg2Of gene,as
R17236 T22646 T22644 arg1Of gene,a
R17237 T22646 T22645 arg1Of gene,housekeeping
R17238 T22647 T22636 arg2Of and,were
R17239 T22648 T22647 arg2Of expressed,and
R17240 T22648 T22649 arg1Of expressed,as
R17241 T22651 T22650 arg1Of fold,relative
R17242 T22652 T22649 arg2Of increase,as
R17243 T22652 T22651 arg1Of increase,fold
R17244 T22652 T22653 arg1Of increase,2
R17245 T22652 T22654 arg1Of increase,(
R17246 T22652 T22657 arg1Of increase,over
R17247 T22655 T22654 arg2Of −ΔΔCt,(
R17248 T22656 T22654 arg3Of ),(
R17249 T22660 T22657 arg2Of samples,over
R17250 T22660 T22658 arg1Of samples,the
R17251 T22660 T22659 arg1Of samples,non-stimulated
R17252 T22660 T22661 arg1Of samples,(
R17253 T22662 T22661 arg2Of NS,(
R17254 T22663 T22661 arg3Of ),(

sentences

Id Subject Object Predicate Lexical cue
T22372 0-44 Sentence denotes RNA Isolation and Quantitative Real-time PCR
T22373 45-179 Sentence denotes MDMs or RAW 264.7 cells were serum-starved overnight or for 2 hours, respectively, prior to incubating with inhibitors for 30 minutes.
T22374 180-382 Sentence denotes The cells were restimulated with 100 ng/ml M-CSF and total mRNA was extracted from cells with Trizol (Invitrogen) and 1–2 µg of total RNA was used to synthesized cDNA using SuperScript III (Invitrogen).
T22375 383-493 Sentence denotes Quantitative real-time (qRT)-PCR was performed using SYBR Green Master Mix (Applied BioSystems, Carlsbad, CA).
T22376 494-620 Sentence denotes The reactions were performed using an ABI PRIZM 7700 machine with software Sequence Detector version 1.7 (Applied Biosystems).
T22377 621-792 Sentence denotes The target gene values were normalized to the values of GAPDH as a housekeeping gene and expressed as relative fold increase 2(−ΔΔCt) over the non-stimulated samples (NS).
T341 0-44 Sentence denotes RNA Isolation and Quantitative Real-time PCR
T342 45-179 Sentence denotes MDMs or RAW 264.7 cells were serum-starved overnight or for 2 hours, respectively, prior to incubating with inhibitors for 30 minutes.
T343 180-382 Sentence denotes The cells were restimulated with 100 ng/ml M-CSF and total mRNA was extracted from cells with Trizol (Invitrogen) and 1–2 µg of total RNA was used to synthesized cDNA using SuperScript III (Invitrogen).
T344 383-493 Sentence denotes Quantitative real-time (qRT)-PCR was performed using SYBR Green Master Mix (Applied BioSystems, Carlsbad, CA).
T345 494-620 Sentence denotes The reactions were performed using an ABI PRIZM 7700 machine with software Sequence Detector version 1.7 (Applied Biosystems).
T346 621-792 Sentence denotes The target gene values were normalized to the values of GAPDH as a housekeeping gene and expressed as relative fold increase 2(−ΔΔCt) over the non-stimulated samples (NS).

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T22378 0-3 NNP denotes RNA
T22379 4-13 NNP denotes Isolation
T22380 14-17 CC denotes and
T22381 18-30 JJ denotes Quantitative
T22382 31-40 JJ denotes Real-time
T22383 41-44 NNP denotes PCR
T22384 45-49 NNPS denotes MDMs
T22385 50-52 CC denotes or
T22386 53-56 NNP denotes RAW
T22387 57-62 CD denotes 264.7
T22388 63-68 NNS denotes cells
T22389 69-73 VBD denotes were
T22390 74-87 JJ denotes serum-starved
T22391 88-97 JJ denotes overnight
T22392 98-100 CC denotes or
T22393 101-104 IN denotes for
T22394 105-106 CD denotes 2
T22395 107-112 NNS denotes hours
T22396 112-113 , denotes ,
T22397 114-126 RB denotes respectively
T22398 126-127 , denotes ,
T22399 128-133 RB denotes prior
T22400 134-136 TO denotes to
T22401 137-147 VBG denotes incubating
T22402 148-152 IN denotes with
T22403 153-163 NNS denotes inhibitors
T22404 164-167 IN denotes for
T22405 168-170 CD denotes 30
T22406 171-178 NNS denotes minutes
T22407 178-179 . denotes .
T22408 180-183 DT denotes The
T22409 184-189 NNS denotes cells
T22410 190-194 VBD denotes were
T22411 195-207 VBN denotes restimulated
T22412 208-212 IN denotes with
T22413 213-216 CD denotes 100
T22414 217-222 JJ denotes ng/ml
T22415 223-228 NNP denotes M-CSF
T22416 229-232 CC denotes and
T22417 233-238 JJ denotes total
T22418 239-243 NNP denotes mRNA
T22419 244-247 VBD denotes was
T22420 248-257 VBN denotes extracted
T22421 258-262 IN denotes from
T22422 263-268 NNS denotes cells
T22423 269-273 IN denotes with
T22424 274-280 NNP denotes Trizol
T22425 281-282 -LRB- denotes (
T22426 282-292 NNP denotes Invitrogen
T22427 292-293 -RRB- denotes )
T22428 294-297 CC denotes and
T22429 298-299 CD denotes 1
T22430 300-301 CD denotes 2
T22431 302-304 NN denotes µg
T22432 305-307 IN denotes of
T22433 308-313 JJ denotes total
T22434 314-317 NNP denotes RNA
T22435 318-321 VBD denotes was
T22436 322-326 VBN denotes used
T22437 327-329 TO denotes to
T22438 330-341 VBN denotes synthesized
T22439 342-346 NNP denotes cDNA
T22440 347-352 VBG denotes using
T22441 353-364 NNP denotes SuperScript
T22442 365-368 NNP denotes III
T22443 369-370 -LRB- denotes (
T22444 370-380 NNP denotes Invitrogen
T22445 380-381 -RRB- denotes )
T22446 381-382 . denotes .
T22447 383-395 JJ denotes Quantitative
T22448 396-405 JJ denotes real-time
T22449 406-407 -LRB- denotes (
T22450 407-410 NNP denotes qRT
T22451 410-411 -RRB- denotes )
T22452 411-412 : denotes -
T22453 412-415 NNP denotes PCR
T22454 416-419 VBD denotes was
T22455 420-429 VBN denotes performed
T22456 430-435 VBG denotes using
T22457 436-440 NNP denotes SYBR
T22458 441-446 NNP denotes Green
T22459 447-453 NNP denotes Master
T22460 454-457 NNP denotes Mix
T22461 458-459 -LRB- denotes (
T22462 459-466 NNP denotes Applied
T22463 467-477 NNPS denotes BioSystems
T22464 477-478 , denotes ,
T22465 479-487 NNP denotes Carlsbad
T22466 487-488 , denotes ,
T22467 489-491 NNP denotes CA
T22468 491-492 -RRB- denotes )
T22469 492-493 . denotes .
T22470 494-497 DT denotes The
T22471 498-507 NNS denotes reactions
T22472 508-512 VBD denotes were
T22473 513-522 VBN denotes performed
T22474 523-528 VBG denotes using
T22475 529-531 DT denotes an
T22476 532-535 NNP denotes ABI
T22477 536-541 NNP denotes PRIZM
T22478 542-546 CD denotes 7700
T22479 547-554 NN denotes machine
T22480 555-559 IN denotes with
T22481 560-568 NN denotes software
T22482 569-577 NNP denotes Sequence
T22483 578-586 NNP denotes Detector
T22484 587-594 NN denotes version
T22485 595-598 CD denotes 1.7
T22486 599-600 -LRB- denotes (
T22487 600-607 NNP denotes Applied
T22488 608-618 NNPS denotes Biosystems
T22489 618-619 -RRB- denotes )
T22490 619-620 . denotes .
T22491 621-624 DT denotes The
T22492 625-631 NN denotes target
T22493 632-636 NN denotes gene
T22494 637-643 NNS denotes values
T22495 644-648 VBD denotes were
T22496 649-659 VBN denotes normalized
T22497 660-662 TO denotes to
T22498 663-666 DT denotes the
T22499 667-673 NNS denotes values
T22500 674-676 IN denotes of
T22501 677-682 NNP denotes GAPDH
T22502 683-685 IN denotes as
T22503 686-687 DT denotes a
T22504 688-700 NN denotes housekeeping
T22505 701-705 NN denotes gene
T22506 706-709 CC denotes and
T22507 710-719 VBD denotes expressed
T22508 720-722 IN denotes as
T22509 723-731 JJ denotes relative
T22510 732-736 VBP denotes fold
T22511 737-745 NN denotes increase
T22512 746-747 CD denotes 2
T22513 747-748 -LRB- denotes (
T22514 748-749 NN denotes −
T22515 749-753 NNP denotes ΔΔCt
T22516 753-754 -RRB- denotes )
T22517 755-759 IN denotes over
T22518 760-763 DT denotes the
T22519 764-778 JJ denotes non-stimulated
T22520 779-786 NNS denotes samples
T22521 787-788 -LRB- denotes (
T22522 788-790 NNP denotes NS
T22523 790-791 -RRB- denotes )
T22524 791-792 . denotes .
R16968 T22378 T22379 compound RNA,Isolation
R16969 T22379 T22379 ROOT Isolation,Isolation
R16970 T22380 T22379 cc and,Isolation
R16971 T22381 T22384 nmod Quantitative,MDMs
R16972 T22382 T22384 amod Real-time,MDMs
R16973 T22383 T22384 compound PCR,MDMs
R16974 T22384 T22379 conj MDMs,Isolation
R16975 T22385 T22384 cc or,MDMs
R16976 T22386 T22384 conj RAW,MDMs
R16977 T22387 T22388 nummod 264.7,cells
R16978 T22388 T22389 nsubj cells,were
R16979 T22389 T22389 ROOT were,were
R16980 T22390 T22391 amod serum-starved,overnight
R16981 T22391 T22389 acomp overnight,were
R16982 T22392 T22389 cc or,were
R16983 T22393 T22399 prep for,prior
R16984 T22394 T22395 nummod 2,hours
R16985 T22395 T22393 pobj hours,for
R16986 T22396 T22399 punct ",",prior
R16987 T22397 T22399 advmod respectively,prior
R16988 T22398 T22399 punct ",",prior
R16989 T22399 T22389 conj prior,were
R16990 T22400 T22399 prep to,prior
R16991 T22401 T22400 pobj incubating,to
R16992 T22402 T22401 prep with,incubating
R16993 T22403 T22402 pobj inhibitors,with
R16994 T22404 T22403 prep for,inhibitors
R16995 T22405 T22406 nummod 30,minutes
R16996 T22406 T22404 pobj minutes,for
R16997 T22407 T22399 punct .,prior
R16998 T22408 T22409 det The,cells
R16999 T22409 T22411 nsubjpass cells,restimulated
R17000 T22410 T22411 auxpass were,restimulated
R17001 T22411 T22411 ROOT restimulated,restimulated
R17002 T22412 T22411 prep with,restimulated
R17003 T22413 T22415 nummod 100,M-CSF
R17004 T22414 T22415 compound ng/ml,M-CSF
R17005 T22415 T22412 pobj M-CSF,with
R17006 T22416 T22415 cc and,M-CSF
R17007 T22417 T22418 amod total,mRNA
R17008 T22418 T22415 conj mRNA,M-CSF
R17009 T22419 T22420 auxpass was,extracted
R17010 T22420 T22411 conj extracted,restimulated
R17011 T22421 T22420 prep from,extracted
R17012 T22422 T22421 pobj cells,from
R17013 T22423 T22422 prep with,cells
R17014 T22424 T22423 pobj Trizol,with
R17015 T22425 T22424 punct (,Trizol
R17016 T22426 T22424 appos Invitrogen,Trizol
R17017 T22427 T22424 punct ),Trizol
R17018 T22428 T22422 cc and,cells
R17019 T22429 T22422 conj 1,cells
R17020 T22430 T22431 nummod 2,µg
R17021 T22431 T22436 nsubjpass µg,used
R17022 T22432 T22431 prep of,µg
R17023 T22433 T22434 amod total,RNA
R17024 T22434 T22432 pobj RNA,of
R17025 T22435 T22436 auxpass was,used
R17026 T22436 T22436 ROOT used,used
R17027 T22437 T22436 prep to,used
R17028 T22438 T22439 amod synthesized,cDNA
R17029 T22439 T22437 pobj cDNA,to
R17030 T22440 T22436 advcl using,used
R17031 T22441 T22442 compound SuperScript,III
R17032 T22442 T22440 dobj III,using
R17033 T22443 T22442 punct (,III
R17034 T22444 T22442 appos Invitrogen,III
R17035 T22445 T22442 punct ),III
R17036 T22446 T22436 punct .,used
R17037 T22447 T22455 amod Quantitative,performed
R17038 T22448 T22455 npadvmod real-time,performed
R17039 T22449 T22450 punct (,qRT
R17040 T22450 T22448 appos qRT,real-time
R17041 T22451 T22450 punct ),qRT
R17042 T22452 T22453 punct -,PCR
R17043 T22453 T22455 nsubjpass PCR,performed
R17044 T22454 T22455 auxpass was,performed
R17045 T22455 T22455 ROOT performed,performed
R17046 T22456 T22455 advcl using,performed
R17047 T22457 T22460 compound SYBR,Mix
R17048 T22458 T22460 compound Green,Mix
R17049 T22459 T22460 compound Master,Mix
R17050 T22460 T22456 dobj Mix,using
R17051 T22461 T22460 punct (,Mix
R17052 T22462 T22463 compound Applied,BioSystems
R17053 T22463 T22460 appos BioSystems,Mix
R17054 T22464 T22463 punct ",",BioSystems
R17055 T22465 T22463 conj Carlsbad,BioSystems
R17056 T22466 T22465 punct ",",Carlsbad
R17057 T22467 T22465 conj CA,Carlsbad
R17058 T22468 T22460 punct ),Mix
R17059 T22469 T22455 punct .,performed
R17060 T22470 T22471 det The,reactions
R17061 T22471 T22473 nsubjpass reactions,performed
R17062 T22472 T22473 auxpass were,performed
R17063 T22473 T22473 ROOT performed,performed
R17064 T22474 T22473 advcl using,performed
R17065 T22475 T22479 det an,machine
R17066 T22476 T22477 compound ABI,PRIZM
R17067 T22477 T22479 nmod PRIZM,machine
R17068 T22478 T22479 nummod 7700,machine
R17069 T22479 T22474 dobj machine,using
R17070 T22480 T22474 prep with,using
R17071 T22481 T22483 compound software,Detector
R17072 T22482 T22483 compound Sequence,Detector
R17073 T22483 T22484 compound Detector,version
R17074 T22484 T22480 pobj version,with
R17075 T22485 T22484 nummod 1.7,version
R17076 T22486 T22488 punct (,Biosystems
R17077 T22487 T22488 compound Applied,Biosystems
R17078 T22488 T22484 appos Biosystems,version
R17079 T22489 T22473 punct ),performed
R17080 T22490 T22473 punct .,performed
R17081 T22491 T22494 det The,values
R17082 T22492 T22493 compound target,gene
R17083 T22493 T22494 compound gene,values
R17084 T22494 T22495 nsubj values,were
R17085 T22495 T22495 ROOT were,were
R17086 T22496 T22495 acomp normalized,were
R17087 T22497 T22496 prep to,normalized
R17088 T22498 T22499 det the,values
R17089 T22499 T22497 pobj values,to
R17090 T22500 T22499 prep of,values
R17091 T22501 T22500 pobj GAPDH,of
R17092 T22502 T22496 prep as,normalized
R17093 T22503 T22505 det a,gene
R17094 T22504 T22505 compound housekeeping,gene
R17095 T22505 T22502 pobj gene,as
R17096 T22506 T22496 cc and,normalized
R17097 T22507 T22496 conj expressed,normalized
R17098 T22508 T22507 prep as,expressed
R17099 T22509 T22511 amod relative,increase
R17100 T22510 T22511 compound fold,increase
R17101 T22511 T22508 pobj increase,as
R17102 T22512 T22511 nummod 2,increase
R17103 T22513 T22515 punct (,ΔΔCt
R17104 T22514 T22515 compound −,ΔΔCt
R17105 T22515 T22511 appos ΔΔCt,increase
R17106 T22516 T22515 punct ),ΔΔCt
R17107 T22517 T22511 prep over,increase
R17108 T22518 T22520 det the,samples
R17109 T22519 T22520 amod non-stimulated,samples
R17110 T22520 T22517 pobj samples,over
R17111 T22521 T22522 punct (,NS
R17112 T22522 T22520 appos NS,samples
R17113 T22523 T22495 punct ),were
R17114 T22524 T22495 punct .,were

test2

Id Subject Object Predicate Lexical cue
T22370 223-228 Protein denotes M-CSF