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PMC:3245220 / 47355-48377 JSONTXT

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bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T22318 12-16 P17252 denotes PKCα
T22319 93-98 P09603 denotes M-CSF
T22320 95-98 P04141 denotes CSF
T22321 328-330 P0A7Z4 denotes pH
T22322 438-442 P17252 denotes PKCα
T22323 528-532 P17252 denotes PKCα

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T22324 12-16 Protein denotes PKCα
T22325 528-532 Protein denotes PKCα
T22326 93-98 Protein denotes M-CSF

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T22362 12-23 Protein denotes PKCα Kinase
T22363 33-43 Protein denotes Human MDMs
T22364 93-98 Protein denotes M-CSF
T22365 317-326 Protein denotes leupeptin
T22366 433-451 Protein denotes anti-PKCα antibody
T22367 528-532 Protein denotes PKCα

events-check-again

Id Subject Object Predicate Lexical cue
T22359 12-16 Protein denotes PKCα
T22360 93-98 Protein denotes M-CSF
T22361 528-532 Protein denotes PKCα

GO-CC

Id Subject Object Predicate Lexical cue
T22340 174-179 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T22341 379-383 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T22342 443-451 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T22343 443-451 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody

GO-MF

Id Subject Object Predicate Lexical cue
T22335 12-16 http://purl.obolibrary.org/obo/GO_0004697 denotes PKCα
T22336 438-442 http://purl.obolibrary.org/obo/GO_0004697 denotes PKCα
T22337 528-532 http://purl.obolibrary.org/obo/GO_0004697 denotes PKCα
T22338 17-32 http://purl.obolibrary.org/obo/GO_0016301 denotes Kinase Activity
T22339 443-451 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody

GO-BP

Id Subject Object Predicate Lexical cue
T22368 17-32 http://purl.obolibrary.org/obo/GO_0016301 denotes Kinase Activity
T22369 200-205 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T22138 0-8 NN denotes Analysis
T22139 9-11 IN denotes of
T22140 12-16 NN denotes PKCα
T22141 17-23 NN denotes Kinase
T22142 24-32 NN denotes Activity
T22143 33-38 JJ denotes Human
T22144 39-43 NN denotes MDMs
T22145 44-48 VB denotes were
T22146 49-56 VB denotes starved
T22147 57-60 IN denotes for
T22148 61-62 CD denotes 2
T22149 63-68 NN denotes hours
T22150 69-75 IN denotes before
T22151 76-87 NN denotes stimulation
T22152 88-92 IN denotes with
T22153 93-98 NN denotes M-CSF
T22154 99-100 -LRB- denotes (
T22155 100-104 CD denotes 0–60
T22156 105-112 NN denotes minutes
T22157 112-113 -RRB- denotes )
T22158 113-114 -COMMA- denotes ,
T22159 115-119 RB denotes then
T22160 120-126 VB denotes washed
T22161 127-131 IN denotes with
T22162 132-136 JJ denotes cold
T22163 137-140 NN denotes PBS
T22164 141-144 CC denotes and
T22165 145-152 VB denotes removed
T22166 153-157 IN denotes from
T22167 158-161 DT denotes the
T22168 162-168 NN denotes plates
T22169 170-173 DT denotes The
T22170 174-179 NN denotes cells
T22171 180-184 VB denotes were
T22172 185-196 VB denotes resuspended
T22173 197-199 IN denotes in
T22174 200-205 NN denotes lysis
T22175 206-212 NN denotes buffer
T22176 213-214 -LRB- denotes (
T22177 214-216 CD denotes 20
T22178 217-219 NN denotes mM
T22179 220-224 NNP denotes Tris
T22180 224-225 -COMMA- denotes ,
T22181 226-227 CD denotes 5
T22182 228-230 NN denotes mM
T22183 231-236 NN denotes MgCl2
T22184 236-237 -COMMA- denotes ,
T22185 238-239 CD denotes 1
T22186 240-242 NN denotes mM
T22187 243-247 NN denotes EGTA
T22188 247-248 -COMMA- denotes ,
T22189 249-251 CD denotes 20
T22190 252-254 NN denotes mM
T22191 255-265 NN denotes β-glycerol
T22192 266-275 NN denotes phosphate
T22193 275-276 -COMMA- denotes ,
T22194 277-278 CD denotes 1
T22195 279-281 NN denotes mM
T22196 282-286 NN denotes PMSF
T22197 287-288 CD denotes 2
T22198 289-294 NN denotes µg/ml
T22199 295-304 NN denotes aprotinin
T22200 305-308 CC denotes and
T22201 309-310 CD denotes 2
T22202 311-316 NN denotes µg/ml
T22203 317-326 NN denotes leupeptin
T22204 326-327 -COMMA- denotes ,
T22205 328-330 NN denotes pH
T22206 331-334 CD denotes 7.5
T22207 334-335 -RRB- denotes )
T22208 335-336 -COMMA- denotes ,
T22209 337-346 VB denotes sonicated
T22210 347-350 CC denotes and
T22211 351-362 VB denotes centrifuged
T22212 363-365 TO denotes to
T22213 366-372 VB denotes obtain
T22214 373-378 JJ denotes whole
T22215 379-383 NN denotes cell
T22216 384-391 NN denotes lysates
T22217 391-392 -COMMA- denotes ,
T22218 393-398 WDT denotes which
T22219 399-403 VB denotes were
T22220 404-408 RB denotes then
T22221 409-427 VB denotes immunoprecipitated
T22222 428-432 IN denotes with
T22223 433-442 JJ denotes anti-PKCα
T22224 443-451 NN denotes antibody
T22225 452-455 CC denotes and
T22226 456-463 NN denotes protein
T22227 464-473 NN denotes G-agarose
T22228 474-475 -LRB- denotes (
T22229 475-485 NN denotes Invitrogen
T22230 485-486 -RRB- denotes )
T22231 488-494 JJ denotes Immune
T22232 495-504 NN denotes complexes
T22233 505-509 VB denotes were
T22234 510-516 VB denotes washed
T22235 517-522 RB denotes twice
T22236 522-523 -COMMA- denotes ,
T22237 524-527 CC denotes and
T22238 528-532 NN denotes PKCα
T22239 533-541 NN denotes activity
T22240 542-545 VB denotes was
T22241 546-554 VB denotes analyzed
T22242 555-557 IN denotes by
T22243 558-573 JJ denotes non-radioactive
T22244 574-580 NN denotes Peptag
T22245 581-586 NN denotes assay
T22246 587-590 NN denotes kit
T22247 591-592 -LRB- denotes (
T22248 592-599 NN denotes Promega
T22249 599-600 -RRB- denotes )
T22250 602-609 RB denotes Briefly
T22251 609-610 -COMMA- denotes ,
T22252 611-617 NN denotes kinase
T22253 618-624 NN denotes buffer
T22254 624-625 -COMMA- denotes ,
T22255 626-635 NN denotes activator
T22256 635-636 -COMMA- denotes ,
T22257 637-644 NN denotes peptide
T22258 645-655 NN denotes protection
T22259 656-664 NN denotes solution
T22260 665-668 CC denotes and
T22261 669-675 NN denotes Peptag
T22262 676-683 NN denotes peptide
T22263 684-688 VB denotes were
T22264 689-698 VB denotes incubated
T22265 699-703 IN denotes with
T22266 704-707 DT denotes the
T22267 708-714 JJ denotes immune
T22268 715-724 NN denotes complexes
T22269 725-727 IN denotes at
T22270 728-732 NN denotes 30°C
T22271 733-736 IN denotes for
T22272 737-739 CD denotes 30
T22273 740-747 NN denotes minutes
T22274 749-758 NN denotes Reactions
T22275 759-763 VB denotes were
T22276 764-771 VB denotes stopped
T22277 772-774 IN denotes by
T22278 775-782 VB denotes boiling
T22279 783-786 IN denotes for
T22280 787-789 CD denotes 10
T22281 790-797 NN denotes minutes
T22282 798-801 CC denotes and
T22283 802-809 NN denotes samples
T22284 810-814 VB denotes were
T22285 815-824 VB denotes separated
T22286 825-827 IN denotes on
T22287 828-831 CD denotes 0.8
T22288 831-832 NN denotes %
T22289 833-840 NN denotes agarose
T22290 841-844 NN denotes gel
T22291 846-860 VB denotes Phosphorylated
T22292 861-868 NN denotes peptide
T22293 869-877 VB denotes migrated
T22294 878-884 IN denotes toward
T22295 885-888 DT denotes the
T22296 889-894 NN denotes anode
T22297 895-896 -LRB- denotes (
T22298 896-897 SYM denotes +
T22299 897-898 -RRB- denotes )
T22300 898-899 -COMMA- denotes ,
T22301 900-905 IN denotes while
T22302 906-924 JJ denotes non-phosphorylated
T22303 925-932 NN denotes peptide
T22304 933-941 VB denotes migrated
T22305 942-948 IN denotes toward
T22306 949-952 DT denotes the
T22307 953-960 NN denotes cathode
T22308 961-962 -LRB- denotes (
T22309 962-963 NN denotes −
T22310 963-964 -RRB- denotes )
T22311 966-984 JJ denotes Fluorescein-tagged
T22312 985-993 NN denotes peptides
T22313 994-998 VB denotes were
T22314 999-1009 VB denotes visualized
T22315 1010-1012 IN denotes by
T22316 1013-1015 NN denotes UV
T22317 1016-1021 NN denotes light
R16783 T22138 T22139 arg1Of Analysis,of
R16784 T22142 T22139 arg2Of Activity,of
R16785 T22142 T22140 arg1Of Activity,PKCα
R16786 T22142 T22141 arg1Of Activity,Kinase
R16787 T22144 T22143 arg1Of MDMs,Human
R16788 T22144 T22145 arg1Of MDMs,were
R16789 T22144 T22146 arg2Of MDMs,starved
R16790 T22146 T22145 arg2Of starved,were
R16791 T22146 T22147 arg1Of starved,for
R16792 T22146 T22150 arg1Of starved,before
R16793 T22149 T22147 arg2Of hours,for
R16794 T22149 T22148 arg1Of hours,2
R16795 T22151 T22150 arg2Of stimulation,before
R16796 T22151 T22152 arg1Of stimulation,with
R16797 T22153 T22152 arg2Of M-CSF,with
R16798 T22153 T22154 arg1Of M-CSF,(
R16799 T22153 T22158 arg1Of M-CSF,","
R16800 T22153 T22160 arg2Of M-CSF,washed
R16801 T22153 T22165 arg2Of M-CSF,removed
R16802 T22156 T22154 arg2Of minutes,(
R16803 T22156 T22155 arg1Of minutes,0–60
R16804 T22157 T22154 arg3Of ),(
R16805 T22160 T22159 arg1Of washed,then
R16806 T22160 T22161 arg1Of washed,with
R16807 T22160 T22164 arg1Of washed,and
R16808 T22163 T22161 arg2Of PBS,with
R16809 T22163 T22162 arg1Of PBS,cold
R16810 T22165 T22164 arg2Of removed,and
R16811 T22165 T22166 arg1Of removed,from
R16812 T22168 T22166 arg2Of plates,from
R16813 T22168 T22167 arg1Of plates,the
R16814 T22170 T22169 arg1Of cells,The
R16815 T22170 T22171 arg1Of cells,were
R16816 T22170 T22172 arg2Of cells,resuspended
R16817 T22170 T22209 arg2Of cells,sonicated
R16818 T22170 T22211 arg2Of cells,centrifuged
R16819 T22170 T22213 arg1Of cells,obtain
R16820 T22172 T22173 arg1Of resuspended,in
R16821 T22172 T22176 arg1Of resuspended,(
R16822 T22172 T22208 arg1Of resuspended,","
R16823 T22175 T22173 arg2Of buffer,in
R16824 T22175 T22174 arg1Of buffer,lysis
R16825 T22179 T22177 arg1Of Tris,20
R16826 T22179 T22178 arg1Of Tris,mM
R16827 T22179 T22180 arg1Of Tris,","
R16828 T22179 T22188 arg1Of Tris,","
R16829 T22181 T22182 arg1Of 5,mM
R16830 T22183 T22180 arg2Of MgCl2,","
R16831 T22183 T22181 arg1Of MgCl2,5
R16832 T22183 T22184 arg1Of MgCl2,","
R16833 T22185 T22186 arg1Of 1,mM
R16834 T22187 T22184 arg2Of EGTA,","
R16835 T22187 T22185 arg1Of EGTA,1
R16836 T22188 T22193 arg1Of ",",","
R16837 T22189 T22190 arg1Of 20,mM
R16838 T22192 T22188 arg2Of phosphate,","
R16839 T22192 T22189 arg1Of phosphate,20
R16840 T22192 T22191 arg1Of phosphate,β-glycerol
R16841 T22193 T22200 arg1Of ",",and
R16842 T22194 T22195 arg1Of 1,mM
R16843 T22197 T22198 arg1Of 2,µg/ml
R16844 T22199 T22193 arg2Of aprotinin,","
R16845 T22199 T22194 arg1Of aprotinin,1
R16846 T22199 T22196 arg1Of aprotinin,PMSF
R16847 T22199 T22197 arg1Of aprotinin,2
R16848 T22200 T22176 arg2Of and,(
R16849 T22203 T22200 arg2Of leupeptin,and
R16850 T22203 T22201 arg1Of leupeptin,2
R16851 T22203 T22202 arg1Of leupeptin,µg/ml
R16852 T22203 T22204 arg1Of leupeptin,","
R16853 T22205 T22204 arg2Of pH,","
R16854 T22205 T22206 arg1Of pH,7.5
R16855 T22207 T22176 arg3Of ),(
R16856 T22208 T22210 arg1Of ",",and
R16857 T22209 T22208 arg2Of sonicated,","
R16858 T22210 T22171 arg2Of and,were
R16859 T22211 T22210 arg2Of centrifuged,and
R16860 T22213 T22211 arg3Of obtain,centrifuged
R16861 T22213 T22212 arg1Of obtain,to
R16862 T22216 T22213 arg2Of lysates,obtain
R16863 T22216 T22214 arg1Of lysates,whole
R16864 T22216 T22215 arg1Of lysates,cell
R16865 T22216 T22217 arg1Of lysates,","
R16866 T22216 T22218 arg1Of lysates,which
R16867 T22216 T22219 arg1Of lysates,were
R16868 T22216 T22221 arg2Of lysates,immunoprecipitated
R16869 T22221 T22219 arg2Of immunoprecipitated,were
R16870 T22221 T22220 arg1Of immunoprecipitated,then
R16871 T22221 T22222 arg1Of immunoprecipitated,with
R16872 T22224 T22223 arg1Of antibody,anti-PKCα
R16873 T22224 T22225 arg1Of antibody,and
R16874 T22225 T22222 arg2Of and,with
R16875 T22227 T22225 arg2Of G-agarose,and
R16876 T22227 T22226 arg1Of G-agarose,protein
R16877 T22227 T22228 arg1Of G-agarose,(
R16878 T22229 T22228 arg2Of Invitrogen,(
R16879 T22230 T22228 arg3Of ),(
R16880 T22232 T22231 arg1Of complexes,Immune
R16881 T22232 T22233 arg1Of complexes,were
R16882 T22232 T22234 arg2Of complexes,washed
R16883 T22234 T22233 arg2Of washed,were
R16884 T22234 T22235 arg1Of washed,twice
R16885 T22234 T22237 arg1Of washed,and
R16886 T22237 T22236 arg1Of and,","
R16887 T22239 T22238 arg1Of activity,PKCα
R16888 T22239 T22240 arg1Of activity,was
R16889 T22239 T22241 arg2Of activity,analyzed
R16890 T22241 T22237 arg2Of analyzed,and
R16891 T22241 T22240 arg2Of analyzed,was
R16892 T22246 T22241 arg1Of kit,analyzed
R16893 T22246 T22242 arg2Of kit,by
R16894 T22246 T22243 arg1Of kit,non-radioactive
R16895 T22246 T22244 arg1Of kit,Peptag
R16896 T22246 T22245 arg1Of kit,assay
R16897 T22246 T22247 arg1Of kit,(
R16898 T22248 T22247 arg2Of Promega,(
R16899 T22249 T22247 arg3Of ),(
R16900 T22253 T22254 arg1Of buffer,","
R16901 T22254 T22256 arg1Of ",",","
R16902 T22255 T22254 arg2Of activator,","
R16903 T22256 T22260 arg1Of ",",and
R16904 T22259 T22256 arg2Of solution,","
R16905 T22259 T22257 arg1Of solution,peptide
R16906 T22259 T22258 arg1Of solution,protection
R16907 T22260 T22252 arg1Of and,kinase
R16908 T22260 T22263 arg1Of and,were
R16909 T22260 T22264 arg2Of and,incubated
R16910 T22262 T22260 arg2Of peptide,and
R16911 T22262 T22261 arg1Of peptide,Peptag
R16912 T22264 T22250 arg1Of incubated,Briefly
R16913 T22264 T22251 arg1Of incubated,","
R16914 T22264 T22263 arg2Of incubated,were
R16915 T22264 T22265 arg1Of incubated,with
R16916 T22268 T22265 arg2Of complexes,with
R16917 T22268 T22266 arg1Of complexes,the
R16918 T22268 T22267 arg1Of complexes,immune
R16919 T22268 T22269 arg1Of complexes,at
R16920 T22268 T22271 arg1Of complexes,for
R16921 T22270 T22269 arg2Of 30°C,at
R16922 T22273 T22271 arg2Of minutes,for
R16923 T22273 T22272 arg1Of minutes,30
R16924 T22274 T22275 arg1Of Reactions,were
R16925 T22274 T22276 arg2Of Reactions,stopped
R16926 T22276 T22275 arg2Of stopped,were
R16927 T22276 T22277 arg1Of stopped,by
R16928 T22276 T22282 arg1Of stopped,and
R16929 T22278 T22277 arg2Of boiling,by
R16930 T22278 T22279 arg1Of boiling,for
R16931 T22281 T22279 arg2Of minutes,for
R16932 T22281 T22280 arg1Of minutes,10
R16933 T22283 T22284 arg1Of samples,were
R16934 T22283 T22285 arg2Of samples,separated
R16935 T22285 T22282 arg2Of separated,and
R16936 T22285 T22284 arg2Of separated,were
R16937 T22285 T22286 arg1Of separated,on
R16938 T22287 T22288 arg1Of 0.8,%
R16939 T22290 T22286 arg2Of gel,on
R16940 T22290 T22287 arg1Of gel,0.8
R16941 T22290 T22289 arg1Of gel,agarose
R16942 T22292 T22291 arg2Of peptide,Phosphorylated
R16943 T22292 T22293 arg1Of peptide,migrated
R16944 T22293 T22294 arg1Of migrated,toward
R16945 T22293 T22300 arg1Of migrated,","
R16946 T22293 T22301 arg1Of migrated,while
R16947 T22296 T22294 arg2Of anode,toward
R16948 T22296 T22295 arg1Of anode,the
R16949 T22296 T22297 arg1Of anode,(
R16950 T22298 T22297 arg2Of +,(
R16951 T22299 T22297 arg3Of ),(
R16952 T22303 T22302 arg1Of peptide,non-phosphorylated
R16953 T22303 T22304 arg1Of peptide,migrated
R16954 T22304 T22301 arg2Of migrated,while
R16955 T22304 T22305 arg1Of migrated,toward
R16956 T22307 T22305 arg2Of cathode,toward
R16957 T22307 T22306 arg1Of cathode,the
R16958 T22307 T22308 arg1Of cathode,(
R16959 T22309 T22308 arg2Of −,(
R16960 T22310 T22308 arg3Of ),(
R16961 T22312 T22311 arg1Of peptides,Fluorescein-tagged
R16962 T22312 T22313 arg1Of peptides,were
R16963 T22312 T22314 arg2Of peptides,visualized
R16964 T22314 T22313 arg2Of visualized,were
R16965 T22317 T22314 arg1Of light,visualized
R16966 T22317 T22315 arg2Of light,by
R16967 T22317 T22316 arg1Of light,UV

sentences

Id Subject Object Predicate Lexical cue
T21936 0-32 Sentence denotes Analysis of PKCα Kinase Activity
T21937 33-169 Sentence denotes Human MDMs were starved for 2 hours before stimulation with M-CSF (0–60 minutes), then washed with cold PBS and removed from the plates.
T21938 170-487 Sentence denotes The cells were resuspended in lysis buffer (20 mM Tris, 5 mM MgCl2, 1 mM EGTA, 20 mM β-glycerol phosphate, 1 mM PMSF 2 µg/ml aprotinin and 2 µg/ml leupeptin, pH 7.5), sonicated and centrifuged to obtain whole cell lysates, which were then immunoprecipitated with anti-PKCα antibody and protein G-agarose (Invitrogen).
T21939 488-601 Sentence denotes Immune complexes were washed twice, and PKCα activity was analyzed by non-radioactive Peptag assay kit (Promega).
T21940 602-748 Sentence denotes Briefly, kinase buffer, activator, peptide protection solution and Peptag peptide were incubated with the immune complexes at 30°C for 30 minutes.
T21941 749-845 Sentence denotes Reactions were stopped by boiling for 10 minutes and samples were separated on 0.8% agarose gel.
T21942 846-965 Sentence denotes Phosphorylated peptide migrated toward the anode (+), while non-phosphorylated peptide migrated toward the cathode (−).
T21943 966-1022 Sentence denotes Fluorescein-tagged peptides were visualized by UV light.
T333 0-32 Sentence denotes Analysis of PKCα Kinase Activity
T334 33-169 Sentence denotes Human MDMs were starved for 2 hours before stimulation with M-CSF (0–60 minutes), then washed with cold PBS and removed from the plates.
T335 170-487 Sentence denotes The cells were resuspended in lysis buffer (20 mM Tris, 5 mM MgCl2, 1 mM EGTA, 20 mM β-glycerol phosphate, 1 mM PMSF 2 µg/ml aprotinin and 2 µg/ml leupeptin, pH 7.5), sonicated and centrifuged to obtain whole cell lysates, which were then immunoprecipitated with anti-PKCα antibody and protein G-agarose (Invitrogen).
T336 488-601 Sentence denotes Immune complexes were washed twice, and PKCα activity was analyzed by non-radioactive Peptag assay kit (Promega).
T337 602-748 Sentence denotes Briefly, kinase buffer, activator, peptide protection solution and Peptag peptide were incubated with the immune complexes at 30°C for 30 minutes.
T338 749-845 Sentence denotes Reactions were stopped by boiling for 10 minutes and samples were separated on 0.8% agarose gel.
T339 846-965 Sentence denotes Phosphorylated peptide migrated toward the anode (+), while non-phosphorylated peptide migrated toward the cathode (−).
T340 966-1022 Sentence denotes Fluorescein-tagged peptides were visualized by UV light.

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T21944 0-8 NN denotes Analysis
T21945 9-11 IN denotes of
T21946 12-16 NNP denotes PKCα
T21947 17-23 NNP denotes Kinase
T21948 24-32 NNP denotes Activity
T21949 33-38 NNP denotes Human
T21950 39-43 NNPS denotes MDMs
T21951 44-48 VBD denotes were
T21952 49-56 VBN denotes starved
T21953 57-60 IN denotes for
T21954 61-62 CD denotes 2
T21955 63-68 NNS denotes hours
T21956 69-75 IN denotes before
T21957 76-87 NN denotes stimulation
T21958 88-92 IN denotes with
T21959 93-98 NNP denotes M-CSF
T21960 99-100 -LRB- denotes (
T21961 100-101 CD denotes 0
T21962 102-104 CD denotes 60
T21963 105-112 NNS denotes minutes
T21964 112-113 -RRB- denotes )
T21965 113-114 , denotes ,
T21966 115-119 RB denotes then
T21967 120-126 VBD denotes washed
T21968 127-131 IN denotes with
T21969 132-136 JJ denotes cold
T21970 137-140 NNP denotes PBS
T21971 141-144 CC denotes and
T21972 145-152 VBN denotes removed
T21973 153-157 IN denotes from
T21974 158-161 DT denotes the
T21975 162-168 NNS denotes plates
T21976 168-169 . denotes .
T21977 170-173 DT denotes The
T21978 174-179 NNS denotes cells
T21979 180-184 VBD denotes were
T21980 185-196 VBN denotes resuspended
T21981 197-199 IN denotes in
T21982 200-205 NN denotes lysis
T21983 206-212 NN denotes buffer
T21984 213-214 -LRB- denotes (
T21985 214-216 CD denotes 20
T21986 217-219 NNP denotes mM
T21987 220-224 NNP denotes Tris
T21988 224-225 , denotes ,
T21989 226-227 CD denotes 5
T21990 228-230 NNP denotes mM
T21991 231-236 NNP denotes MgCl2
T21992 236-237 , denotes ,
T21993 238-239 CD denotes 1
T21994 240-242 NNP denotes mM
T21995 243-247 NNP denotes EGTA
T21996 247-248 , denotes ,
T21997 249-251 CD denotes 20
T21998 252-254 NNP denotes mM
T21999 255-265 JJ denotes β-glycerol
T22000 266-275 NN denotes phosphate
T22001 275-276 , denotes ,
T22002 277-278 CD denotes 1
T22003 279-281 NNP denotes mM
T22004 282-286 NNP denotes PMSF
T22005 287-288 CD denotes 2
T22006 289-291 NN denotes µg
T22007 291-292 NN denotes /
T22008 292-294 NN denotes ml
T22009 295-304 NN denotes aprotinin
T22010 305-308 CC denotes and
T22011 309-310 CD denotes 2
T22012 311-313 NN denotes µg
T22013 313-314 NN denotes /
T22014 314-316 NN denotes ml
T22015 317-326 NN denotes leupeptin
T22016 326-327 , denotes ,
T22017 328-330 NNP denotes pH
T22018 331-334 CD denotes 7.5
T22019 334-335 -RRB- denotes )
T22020 335-336 , denotes ,
T22021 337-346 VBN denotes sonicated
T22022 347-350 CC denotes and
T22023 351-362 VBN denotes centrifuged
T22024 363-365 TO denotes to
T22025 366-372 VB denotes obtain
T22026 373-378 JJ denotes whole
T22027 379-383 NN denotes cell
T22028 384-391 NNS denotes lysates
T22029 391-392 , denotes ,
T22030 393-398 WDT denotes which
T22031 399-403 VBD denotes were
T22032 404-408 RB denotes then
T22033 409-427 VBN denotes immunoprecipitated
T22034 428-432 IN denotes with
T22035 433-442 JJ denotes anti-PKCα
T22036 443-451 NN denotes antibody
T22037 452-455 CC denotes and
T22038 456-463 NN denotes protein
T22039 464-473 NN denotes G-agarose
T22040 474-475 -LRB- denotes (
T22041 475-485 NNP denotes Invitrogen
T22042 485-486 -RRB- denotes )
T22043 486-487 . denotes .
T22044 488-494 NNP denotes Immune
T22045 495-504 NNS denotes complexes
T22046 505-509 VBD denotes were
T22047 510-516 VBN denotes washed
T22048 517-522 RB denotes twice
T22049 522-523 , denotes ,
T22050 524-527 CC denotes and
T22051 528-532 NNP denotes PKCα
T22052 533-541 NN denotes activity
T22053 542-545 VBD denotes was
T22054 546-554 VBN denotes analyzed
T22055 555-557 IN denotes by
T22056 558-573 JJ denotes non-radioactive
T22057 574-580 NNP denotes Peptag
T22058 581-586 NN denotes assay
T22059 587-590 NN denotes kit
T22060 591-592 -LRB- denotes (
T22061 592-599 NNP denotes Promega
T22062 599-600 -RRB- denotes )
T22063 600-601 . denotes .
T22064 602-609 RB denotes Briefly
T22065 609-610 , denotes ,
T22066 611-617 NN denotes kinase
T22067 618-624 NN denotes buffer
T22068 624-625 , denotes ,
T22069 626-635 NN denotes activator
T22070 635-636 , denotes ,
T22071 637-644 NN denotes peptide
T22072 645-655 NN denotes protection
T22073 656-664 NN denotes solution
T22074 665-668 CC denotes and
T22075 669-675 NNP denotes Peptag
T22076 676-683 NN denotes peptide
T22077 684-688 VBD denotes were
T22078 689-698 VBN denotes incubated
T22079 699-703 IN denotes with
T22080 704-707 DT denotes the
T22081 708-714 JJ denotes immune
T22082 715-724 NNS denotes complexes
T22083 725-727 IN denotes at
T22084 728-730 CD denotes 30
T22085 730-731 CD denotes °
T22086 731-732 NNP denotes C
T22087 733-736 IN denotes for
T22088 737-739 CD denotes 30
T22089 740-747 NNS denotes minutes
T22090 747-748 . denotes .
T22091 749-758 NNS denotes Reactions
T22092 759-763 VBD denotes were
T22093 764-771 VBN denotes stopped
T22094 772-774 IN denotes by
T22095 775-782 VBG denotes boiling
T22096 783-786 IN denotes for
T22097 787-789 CD denotes 10
T22098 790-797 NNS denotes minutes
T22099 798-801 CC denotes and
T22100 802-809 NNS denotes samples
T22101 810-814 VBD denotes were
T22102 815-824 VBN denotes separated
T22103 825-827 IN denotes on
T22104 828-831 CD denotes 0.8
T22105 831-832 NN denotes %
T22106 833-840 JJ denotes agarose
T22107 841-844 NN denotes gel
T22108 844-845 . denotes .
T22109 846-860 NNP denotes Phosphorylated
T22110 861-868 NN denotes peptide
T22111 869-877 VBD denotes migrated
T22112 878-884 IN denotes toward
T22113 885-888 DT denotes the
T22114 889-894 NN denotes anode
T22115 895-896 -LRB- denotes (
T22116 896-897 FW denotes +
T22117 897-898 -RRB- denotes )
T22118 898-899 , denotes ,
T22119 900-905 IN denotes while
T22120 906-924 JJ denotes non-phosphorylated
T22121 925-932 NN denotes peptide
T22122 933-941 VBD denotes migrated
T22123 942-948 IN denotes toward
T22124 949-952 DT denotes the
T22125 953-960 NN denotes cathode
T22126 961-962 -LRB- denotes (
T22127 962-963 FW denotes −
T22128 963-964 -RRB- denotes )
T22129 964-965 . denotes .
T22130 966-984 JJ denotes Fluorescein-tagged
T22131 985-993 NNS denotes peptides
T22132 994-998 VBD denotes were
T22133 999-1009 VBN denotes visualized
T22134 1010-1012 IN denotes by
T22135 1013-1015 NNP denotes UV
T22136 1016-1021 NN denotes light
T22137 1021-1022 . denotes .
R16589 T21944 T21952 nsubjpass Analysis,starved
R16590 T21945 T21944 prep of,Analysis
R16591 T21946 T21950 compound PKCα,MDMs
R16592 T21947 T21950 compound Kinase,MDMs
R16593 T21948 T21950 compound Activity,MDMs
R16594 T21949 T21950 compound Human,MDMs
R16595 T21950 T21945 pobj MDMs,of
R16596 T21951 T21952 auxpass were,starved
R16597 T21952 T21952 ROOT starved,starved
R16598 T21953 T21952 prep for,starved
R16599 T21954 T21955 nummod 2,hours
R16600 T21955 T21953 pobj hours,for
R16601 T21956 T21952 prep before,starved
R16602 T21957 T21956 pobj stimulation,before
R16603 T21958 T21957 prep with,stimulation
R16604 T21959 T21958 pobj M-CSF,with
R16605 T21960 T21959 punct (,M-CSF
R16606 T21961 T21959 nummod 0,M-CSF
R16607 T21962 T21963 nummod 60,minutes
R16608 T21963 T21959 appos minutes,M-CSF
R16609 T21964 T21959 punct ),M-CSF
R16610 T21965 T21967 punct ",",washed
R16611 T21966 T21967 advmod then,washed
R16612 T21967 T21952 dep washed,starved
R16613 T21968 T21967 prep with,washed
R16614 T21969 T21970 amod cold,PBS
R16615 T21970 T21968 pobj PBS,with
R16616 T21971 T21967 cc and,washed
R16617 T21972 T21967 conj removed,washed
R16618 T21973 T21972 prep from,removed
R16619 T21974 T21975 det the,plates
R16620 T21975 T21973 pobj plates,from
R16621 T21976 T21967 punct .,washed
R16622 T21977 T21978 det The,cells
R16623 T21978 T21980 nsubjpass cells,resuspended
R16624 T21979 T21980 auxpass were,resuspended
R16625 T21980 T21980 ROOT resuspended,resuspended
R16626 T21981 T21980 prep in,resuspended
R16627 T21982 T21983 compound lysis,buffer
R16628 T21983 T21981 pobj buffer,in
R16629 T21984 T21987 punct (,Tris
R16630 T21985 T21987 nummod 20,Tris
R16631 T21986 T21987 compound mM,Tris
R16632 T21987 T21987 ROOT Tris,Tris
R16633 T21988 T21987 punct ",",Tris
R16634 T21989 T21991 nummod 5,MgCl2
R16635 T21990 T21991 compound mM,MgCl2
R16636 T21991 T21987 appos MgCl2,Tris
R16637 T21992 T21991 punct ",",MgCl2
R16638 T21993 T21995 nummod 1,EGTA
R16639 T21994 T21995 compound mM,EGTA
R16640 T21995 T21991 appos EGTA,MgCl2
R16641 T21996 T21995 punct ",",EGTA
R16642 T21997 T22000 nummod 20,phosphate
R16643 T21998 T22000 nmod mM,phosphate
R16644 T21999 T22000 amod β-glycerol,phosphate
R16645 T22000 T21995 appos phosphate,EGTA
R16646 T22001 T22000 punct ",",phosphate
R16647 T22002 T22021 nsubj 1,sonicated
R16648 T22003 T22009 nmod mM,aprotinin
R16649 T22004 T22009 nmod PMSF,aprotinin
R16650 T22005 T22006 nummod 2,µg
R16651 T22006 T22009 nmod µg,aprotinin
R16652 T22007 T22009 nmod /,aprotinin
R16653 T22008 T22009 compound ml,aprotinin
R16654 T22009 T22021 nsubj aprotinin,sonicated
R16655 T22010 T22009 cc and,aprotinin
R16656 T22011 T22012 nummod 2,µg
R16657 T22012 T22015 nmod µg,leupeptin
R16658 T22013 T22015 nmod /,leupeptin
R16659 T22014 T22015 compound ml,leupeptin
R16660 T22015 T22009 conj leupeptin,aprotinin
R16661 T22016 T22015 punct ",",leupeptin
R16662 T22017 T22015 appos pH,leupeptin
R16663 T22018 T22017 nummod 7.5,pH
R16664 T22019 T22015 punct ),leupeptin
R16665 T22020 T22015 punct ",",leupeptin
R16666 T22021 T22000 conj sonicated,phosphate
R16667 T22022 T22021 cc and,sonicated
R16668 T22023 T22021 conj centrifuged,sonicated
R16669 T22024 T22025 aux to,obtain
R16670 T22025 T22023 advcl obtain,centrifuged
R16671 T22026 T22028 amod whole,lysates
R16672 T22027 T22028 compound cell,lysates
R16673 T22028 T22025 dobj lysates,obtain
R16674 T22029 T22028 punct ",",lysates
R16675 T22030 T22033 nsubjpass which,immunoprecipitated
R16676 T22031 T22033 auxpass were,immunoprecipitated
R16677 T22032 T22033 advmod then,immunoprecipitated
R16678 T22033 T22028 relcl immunoprecipitated,lysates
R16679 T22034 T22033 prep with,immunoprecipitated
R16680 T22035 T22036 amod anti-PKCα,antibody
R16681 T22036 T22034 pobj antibody,with
R16682 T22037 T22036 cc and,antibody
R16683 T22038 T22039 compound protein,G-agarose
R16684 T22039 T22036 conj G-agarose,antibody
R16685 T22040 T22041 punct (,Invitrogen
R16686 T22041 T22039 appos Invitrogen,G-agarose
R16687 T22042 T22039 punct ),G-agarose
R16688 T22043 T22021 punct .,sonicated
R16689 T22044 T22045 compound Immune,complexes
R16690 T22045 T22047 nsubjpass complexes,washed
R16691 T22046 T22047 auxpass were,washed
R16692 T22047 T22047 ROOT washed,washed
R16693 T22048 T22047 advmod twice,washed
R16694 T22049 T22047 punct ",",washed
R16695 T22050 T22047 cc and,washed
R16696 T22051 T22052 compound PKCα,activity
R16697 T22052 T22054 nsubjpass activity,analyzed
R16698 T22053 T22054 auxpass was,analyzed
R16699 T22054 T22047 conj analyzed,washed
R16700 T22055 T22054 agent by,analyzed
R16701 T22056 T22059 amod non-radioactive,kit
R16702 T22057 T22059 compound Peptag,kit
R16703 T22058 T22059 compound assay,kit
R16704 T22059 T22055 pobj kit,by
R16705 T22060 T22059 punct (,kit
R16706 T22061 T22059 appos Promega,kit
R16707 T22062 T22059 punct ),kit
R16708 T22063 T22054 punct .,analyzed
R16709 T22064 T22078 advmod Briefly,incubated
R16710 T22065 T22078 punct ",",incubated
R16711 T22066 T22067 compound kinase,buffer
R16712 T22067 T22078 nsubjpass buffer,incubated
R16713 T22068 T22067 punct ",",buffer
R16714 T22069 T22067 conj activator,buffer
R16715 T22070 T22069 punct ",",activator
R16716 T22071 T22072 compound peptide,protection
R16717 T22072 T22073 compound protection,solution
R16718 T22073 T22069 conj solution,activator
R16719 T22074 T22073 cc and,solution
R16720 T22075 T22076 compound Peptag,peptide
R16721 T22076 T22073 conj peptide,solution
R16722 T22077 T22078 auxpass were,incubated
R16723 T22078 T22078 ROOT incubated,incubated
R16724 T22079 T22078 prep with,incubated
R16725 T22080 T22082 det the,complexes
R16726 T22081 T22082 amod immune,complexes
R16727 T22082 T22079 pobj complexes,with
R16728 T22083 T22082 prep at,complexes
R16729 T22084 T22085 compound 30,°
R16730 T22085 T22086 nummod °,C
R16731 T22086 T22083 pobj C,at
R16732 T22087 T22078 prep for,incubated
R16733 T22088 T22089 nummod 30,minutes
R16734 T22089 T22087 pobj minutes,for
R16735 T22090 T22078 punct .,incubated
R16736 T22091 T22093 nsubjpass Reactions,stopped
R16737 T22092 T22093 auxpass were,stopped
R16738 T22093 T22093 ROOT stopped,stopped
R16739 T22094 T22093 prep by,stopped
R16740 T22095 T22094 pcomp boiling,by
R16741 T22096 T22095 prep for,boiling
R16742 T22097 T22098 nummod 10,minutes
R16743 T22098 T22096 pobj minutes,for
R16744 T22099 T22098 cc and,minutes
R16745 T22100 T22098 conj samples,minutes
R16746 T22101 T22102 auxpass were,separated
R16747 T22102 T22093 advcl separated,stopped
R16748 T22103 T22102 prep on,separated
R16749 T22104 T22105 nummod 0.8,%
R16750 T22105 T22107 nmod %,gel
R16751 T22106 T22107 amod agarose,gel
R16752 T22107 T22103 pobj gel,on
R16753 T22108 T22093 punct .,stopped
R16754 T22109 T22110 compound Phosphorylated,peptide
R16755 T22110 T22111 nsubj peptide,migrated
R16756 T22111 T22111 ROOT migrated,migrated
R16757 T22112 T22111 prep toward,migrated
R16758 T22113 T22114 det the,anode
R16759 T22114 T22112 pobj anode,toward
R16760 T22115 T22114 punct (,anode
R16761 T22116 T22114 parataxis +,anode
R16762 T22117 T22114 punct ),anode
R16763 T22118 T22111 punct ",",migrated
R16764 T22119 T22122 mark while,migrated
R16765 T22120 T22121 compound non-phosphorylated,peptide
R16766 T22121 T22122 nsubj peptide,migrated
R16767 T22122 T22111 advcl migrated,migrated
R16768 T22123 T22122 prep toward,migrated
R16769 T22124 T22125 det the,cathode
R16770 T22125 T22123 pobj cathode,toward
R16771 T22126 T22125 punct (,cathode
R16772 T22127 T22125 appos −,cathode
R16773 T22128 T22125 punct ),cathode
R16774 T22129 T22111 punct .,migrated
R16775 T22130 T22131 amod Fluorescein-tagged,peptides
R16776 T22131 T22133 nsubjpass peptides,visualized
R16777 T22132 T22133 auxpass were,visualized
R16778 T22133 T22133 ROOT visualized,visualized
R16779 T22134 T22133 agent by,visualized
R16780 T22135 T22136 compound UV,light
R16781 T22136 T22134 pobj light,by
R16782 T22137 T22133 punct .,visualized

test2

Id Subject Object Predicate Lexical cue
T21933 12-16 Protein denotes PKCα
T21934 93-98 Protein denotes M-CSF
T21935 528-532 Protein denotes PKCα