PMC:3245220 / 46800-47353
Annnotations
bionlp-st-ge-2016-reference-tees
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T21930 | 250-261 | Protein | denotes | BCA protein |
GO-CC
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T21928 | 68-72 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cell |
| T21929 | 401-410 | http://purl.obolibrary.org/obo/GO_0016020 | denotes | membranes |
GO-MF
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T21927 | 288-290 | http://purl.obolibrary.org/obo/GO_0033968 | denotes | CA |
GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T21925 | 73-78 | http://purl.obolibrary.org/obo/GO_0019835 | denotes | lysis |
| T21926 | 288-290 | http://purl.obolibrary.org/obo/GO_0033968 | denotes | CA |
pmc-enju-pas
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T21830 | 0-7 | NNP | denotes | Western |
| T21831 | 8-12 | NNP | denotes | Blot |
| T21832 | 13-21 | NN | denotes | Analysis |
| T21833 | 22-27 | NN | denotes | Cells |
| T21834 | 28-32 | VB | denotes | were |
| T21835 | 33-39 | VB | denotes | washed |
| T21836 | 40-44 | IN | denotes | with |
| T21837 | 45-48 | NNP | denotes | PBS |
| T21838 | 49-52 | CC | denotes | and |
| T21839 | 53-64 | VB | denotes | resuspended |
| T21840 | 65-67 | IN | denotes | in |
| T21841 | 68-72 | NN | denotes | cell |
| T21842 | 73-78 | NN | denotes | lysis |
| T21843 | 79-86 | NN | denotes | buffer. |
| T21844 | 87-88 | -LRB- | denotes | ( |
| T21845 | 88-92 | NNP | denotes | Cell |
| T21846 | 93-102 | NNP | denotes | Signaling |
| T21847 | 103-113 | NNP | denotes | Technology |
| T21848 | 113-114 | -RRB- | denotes | ) |
| T21849 | 115-118 | CC | denotes | and |
| T21850 | 119-128 | VB | denotes | incubated |
| T21851 | 129-132 | IN | denotes | for |
| T21852 | 133-135 | CD | denotes | 10 |
| T21853 | 136-143 | NN | denotes | minutes |
| T21854 | 144-146 | IN | denotes | on |
| T21855 | 147-150 | NN | denotes | ice |
| T21856 | 151-155 | RB | denotes | then |
| T21857 | 156-167 | VB | denotes | centrifuged |
| T21858 | 168-170 | TO | denotes | to |
| T21859 | 171-177 | VB | denotes | remove |
| T21860 | 178-181 | DT | denotes | the |
| T21861 | 182-191 | JJ | denotes | insoluble |
| T21862 | 192-200 | NN | denotes | fraction |
| T21863 | 202-205 | DT | denotes | The |
| T21864 | 206-213 | NN | denotes | protein |
| T21865 | 214-227 | NN | denotes | concentration |
| T21866 | 228-231 | VB | denotes | was |
| T21867 | 232-242 | VB | denotes | determined |
| T21868 | 243-245 | IN | denotes | by |
| T21869 | 246-249 | DT | denotes | the |
| T21870 | 250-253 | NN | denotes | BCA |
| T21871 | 254-261 | NN | denotes | protein |
| T21872 | 262-267 | NN | denotes | assay |
| T21873 | 268-269 | -LRB- | denotes | ( |
| T21874 | 269-276 | NNP | denotes | Bio-Rad |
| T21875 | 276-277 | -COMMA- | denotes | , |
| T21876 | 278-286 | NNP | denotes | Hercules |
| T21877 | 286-287 | -COMMA- | denotes | , |
| T21878 | 288-290 | NNP | denotes | CA |
| T21879 | 290-291 | -RRB- | denotes | ) |
| T21880 | 293-297 | NN | denotes | Cell |
| T21881 | 298-305 | NN | denotes | lysates |
| T21882 | 306-310 | VB | denotes | were |
| T21883 | 311-320 | VB | denotes | separated |
| T21884 | 321-323 | IN | denotes | by |
| T21885 | 324-332 | NN | denotes | SDS-PAGE |
| T21886 | 333-335 | IN | denotes | on |
| T21887 | 336-338 | CD | denotes | 10 |
| T21888 | 338-339 | NN | denotes | % |
| T21889 | 340-354 | NN | denotes | polyacrylamide |
| T21890 | 355-359 | NN | denotes | gels |
| T21891 | 360-363 | CC | denotes | and |
| T21892 | 364-368 | RB | denotes | then |
| T21893 | 369-380 | VB | denotes | transferred |
| T21894 | 381-385 | IN | denotes | onto |
| T21895 | 386-400 | NN | denotes | nitrocellulose |
| T21896 | 401-410 | NN | denotes | membranes |
| T21897 | 411-414 | CC | denotes | and |
| T21898 | 415-424 | VB | denotes | subjected |
| T21899 | 425-427 | TO | denotes | to |
| T21900 | 428-435 | JJ | denotes | Western |
| T21901 | 436-444 | NN | denotes | blotting |
| T21902 | 446-449 | DT | denotes | The |
| T21903 | 450-463 | JJ | denotes | immunoblotted |
| T21904 | 464-472 | NN | denotes | proteins |
| T21905 | 473-477 | VB | denotes | were |
| T21906 | 478-486 | VB | denotes | detected |
| T21907 | 487-489 | IN | denotes | by |
| T21908 | 490-493 | NN | denotes | ECL |
| T21909 | 494-501 | NN | denotes | reagent |
| T21910 | 502-503 | -LRB- | denotes | ( |
| T21911 | 503-505 | NNP | denotes | GE |
| T21912 | 506-516 | NNP | denotes | Healthcare |
| T21913 | 517-529 | NNP | denotes | Bio-Sciences |
| T21914 | 530-535 | NNP | denotes | Corp. |
| T21915 | 535-536 | -COMMA- | denotes | , |
| T21916 | 537-547 | NNP | denotes | Piscataway |
| T21917 | 547-548 | -COMMA- | denotes | , |
| T21918 | 549-551 | NNP | denotes | NJ |
| T21919 | 551-552 | -RRB- | denotes | ) |
| R16495 | T21831 | T21830 | arg1Of | Blot,Western |
| R16496 | T21832 | T21831 | arg1Of | Analysis,Blot |
| R16497 | T21833 | T21839 | arg1Of | Cells,resuspended |
| R16498 | T21833 | T21834 | arg1Of | Cells,were |
| R16499 | T21833 | T21835 | arg2Of | Cells,washed |
| R16500 | T21833 | T21850 | arg2Of | Cells,incubated |
| R16501 | T21835 | T21836 | arg1Of | washed,with |
| R16502 | T21835 | T21838 | arg1Of | washed,and |
| R16503 | T21837 | T21836 | arg2Of | PBS,with |
| R16504 | T21838 | T21849 | arg1Of | and,and |
| R16505 | T21839 | T21838 | arg2Of | resuspended,and |
| R16506 | T21839 | T21840 | arg1Of | resuspended,in |
| R16507 | T21843 | T21840 | arg2Of | buffer.,in |
| R16508 | T21843 | T21841 | arg1Of | buffer.,cell |
| R16509 | T21843 | T21842 | arg1Of | buffer.,lysis |
| R16510 | T21843 | T21844 | arg1Of | buffer.,( |
| R16511 | T21847 | T21844 | arg2Of | Technology,( |
| R16512 | T21847 | T21845 | arg1Of | Technology,Cell |
| R16513 | T21847 | T21846 | arg1Of | Technology,Signaling |
| R16514 | T21848 | T21844 | arg3Of | ),( |
| R16515 | T21849 | T21834 | arg2Of | and,were |
| R16516 | T21850 | T21849 | arg2Of | incubated,and |
| R16517 | T21850 | T21851 | arg1Of | incubated,for |
| R16518 | T21853 | T21851 | arg2Of | minutes,for |
| R16519 | T21853 | T21852 | arg1Of | minutes,10 |
| R16520 | T21853 | T21854 | arg1Of | minutes,on |
| R16521 | T21855 | T21854 | arg2Of | ice,on |
| R16522 | T21855 | T21857 | arg2Of | ice,centrifuged |
| R16523 | T21855 | T21859 | arg1Of | ice,remove |
| R16524 | T21857 | T21856 | arg1Of | centrifuged,then |
| R16525 | T21859 | T21857 | arg3Of | remove,centrifuged |
| R16526 | T21859 | T21858 | arg1Of | remove,to |
| R16527 | T21862 | T21859 | arg2Of | fraction,remove |
| R16528 | T21862 | T21860 | arg1Of | fraction,the |
| R16529 | T21862 | T21861 | arg1Of | fraction,insoluble |
| R16530 | T21865 | T21863 | arg1Of | concentration,The |
| R16531 | T21865 | T21864 | arg1Of | concentration,protein |
| R16532 | T21865 | T21866 | arg1Of | concentration,was |
| R16533 | T21865 | T21867 | arg2Of | concentration,determined |
| R16534 | T21867 | T21866 | arg2Of | determined,was |
| R16535 | T21867 | T21873 | arg1Of | determined,( |
| R16536 | T21872 | T21867 | arg1Of | assay,determined |
| R16537 | T21872 | T21868 | arg2Of | assay,by |
| R16538 | T21872 | T21869 | arg1Of | assay,the |
| R16539 | T21872 | T21870 | arg1Of | assay,BCA |
| R16540 | T21872 | T21871 | arg1Of | assay,protein |
| R16541 | T21874 | T21875 | arg1Of | Bio-Rad,"," |
| R16542 | T21875 | T21873 | arg2Of | ",",( |
| R16543 | T21875 | T21877 | arg1Of | ",","," |
| R16544 | T21876 | T21875 | arg2Of | Hercules,"," |
| R16545 | T21878 | T21877 | arg2Of | CA,"," |
| R16546 | T21879 | T21873 | arg3Of | ),( |
| R16547 | T21881 | T21880 | arg1Of | lysates,Cell |
| R16548 | T21881 | T21882 | arg1Of | lysates,were |
| R16549 | T21881 | T21883 | arg2Of | lysates,separated |
| R16550 | T21881 | T21893 | arg2Of | lysates,transferred |
| R16551 | T21881 | T21898 | arg2Of | lysates,subjected |
| R16552 | T21883 | T21886 | arg1Of | separated,on |
| R16553 | T21883 | T21891 | arg1Of | separated,and |
| R16554 | T21885 | T21883 | arg1Of | SDS-PAGE,separated |
| R16555 | T21885 | T21884 | arg2Of | SDS-PAGE,by |
| R16556 | T21887 | T21888 | arg1Of | 10,% |
| R16557 | T21890 | T21886 | arg2Of | gels,on |
| R16558 | T21890 | T21887 | arg1Of | gels,10 |
| R16559 | T21890 | T21889 | arg1Of | gels,polyacrylamide |
| R16560 | T21891 | T21882 | arg2Of | and,were |
| R16561 | T21893 | T21894 | arg1Of | transferred,onto |
| R16562 | T21893 | T21897 | arg1Of | transferred,and |
| R16563 | T21896 | T21894 | arg2Of | membranes,onto |
| R16564 | T21896 | T21895 | arg1Of | membranes,nitrocellulose |
| R16565 | T21897 | T21891 | arg2Of | and,and |
| R16566 | T21897 | T21892 | arg1Of | and,then |
| R16567 | T21898 | T21897 | arg2Of | subjected,and |
| R16568 | T21898 | T21899 | arg1Of | subjected,to |
| R16569 | T21901 | T21899 | arg2Of | blotting,to |
| R16570 | T21901 | T21900 | arg1Of | blotting,Western |
| R16571 | T21904 | T21902 | arg1Of | proteins,The |
| R16572 | T21904 | T21903 | arg1Of | proteins,immunoblotted |
| R16573 | T21904 | T21905 | arg1Of | proteins,were |
| R16574 | T21904 | T21906 | arg2Of | proteins,detected |
| R16575 | T21906 | T21905 | arg2Of | detected,were |
| R16576 | T21906 | T21910 | arg1Of | detected,( |
| R16577 | T21909 | T21906 | arg1Of | reagent,detected |
| R16578 | T21909 | T21907 | arg2Of | reagent,by |
| R16579 | T21909 | T21908 | arg1Of | reagent,ECL |
| R16580 | T21914 | T21910 | arg2Of | Corp.,( |
| R16581 | T21914 | T21911 | arg1Of | Corp.,GE |
| R16582 | T21914 | T21912 | arg1Of | Corp.,Healthcare |
| R16583 | T21914 | T21913 | arg1Of | Corp.,Bio-Sciences |
| R16584 | T21914 | T21915 | arg1Of | Corp.,"," |
| R16585 | T21916 | T21915 | arg2Of | Piscataway,"," |
| R16586 | T21916 | T21917 | arg1Of | Piscataway,"," |
| R16587 | T21918 | T21917 | arg2Of | NJ,"," |
| R16588 | T21919 | T21910 | arg3Of | ),( |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T21730 | 0-21 | Sentence | denotes | Western Blot Analysis |
| T21731 | 22-201 | Sentence | denotes | Cells were washed with PBS and resuspended in cell lysis buffer. (Cell Signaling Technology) and incubated for 10 minutes on ice then centrifuged to remove the insoluble fraction. |
| T21732 | 202-292 | Sentence | denotes | The protein concentration was determined by the BCA protein assay (Bio-Rad, Hercules, CA). |
| T21733 | 293-445 | Sentence | denotes | Cell lysates were separated by SDS-PAGE on 10% polyacrylamide gels and then transferred onto nitrocellulose membranes and subjected to Western blotting. |
| T21734 | 446-553 | Sentence | denotes | The immunoblotted proteins were detected by ECL reagent (GE Healthcare Bio-Sciences Corp., Piscataway, NJ). |
| T328 | 0-21 | Sentence | denotes | Western Blot Analysis |
| T329 | 22-201 | Sentence | denotes | Cells were washed with PBS and resuspended in cell lysis buffer. (Cell Signaling Technology) and incubated for 10 minutes on ice then centrifuged to remove the insoluble fraction. |
| T330 | 202-292 | Sentence | denotes | The protein concentration was determined by the BCA protein assay (Bio-Rad, Hercules, CA). |
| T331 | 293-445 | Sentence | denotes | Cell lysates were separated by SDS-PAGE on 10% polyacrylamide gels and then transferred onto nitrocellulose membranes and subjected to Western blotting. |
| T332 | 446-553 | Sentence | denotes | The immunoblotted proteins were detected by ECL reagent (GE Healthcare Bio-Sciences Corp., Piscataway, NJ). |
bionlp-st-ge-2016-spacy-parsed
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T21735 | 0-7 | JJ | denotes | Western |
| T21736 | 8-12 | NNP | denotes | Blot |
| T21737 | 13-21 | NNP | denotes | Analysis |
| T21738 | 22-27 | NNPS | denotes | Cells |
| T21739 | 28-32 | VBD | denotes | were |
| T21740 | 33-39 | VBN | denotes | washed |
| T21741 | 40-44 | IN | denotes | with |
| T21742 | 45-48 | NNP | denotes | PBS |
| T21743 | 49-52 | CC | denotes | and |
| T21744 | 53-64 | VBD | denotes | resuspended |
| T21745 | 65-67 | IN | denotes | in |
| T21746 | 68-72 | NN | denotes | cell |
| T21747 | 73-78 | NN | denotes | lysis |
| T21748 | 79-85 | NN | denotes | buffer |
| T21749 | 85-86 | . | denotes | . |
| T21750 | 87-88 | -LRB- | denotes | ( |
| T21751 | 88-92 | NNP | denotes | Cell |
| T21752 | 93-102 | NNP | denotes | Signaling |
| T21753 | 103-113 | NNP | denotes | Technology |
| T21754 | 113-114 | -RRB- | denotes | ) |
| T21755 | 115-118 | CC | denotes | and |
| T21756 | 119-128 | VBN | denotes | incubated |
| T21757 | 129-132 | IN | denotes | for |
| T21758 | 133-135 | CD | denotes | 10 |
| T21759 | 136-143 | NNS | denotes | minutes |
| T21760 | 144-146 | IN | denotes | on |
| T21761 | 147-150 | NN | denotes | ice |
| T21762 | 151-155 | RB | denotes | then |
| T21763 | 156-167 | VBD | denotes | centrifuged |
| T21764 | 168-170 | TO | denotes | to |
| T21765 | 171-177 | VB | denotes | remove |
| T21766 | 178-181 | DT | denotes | the |
| T21767 | 182-191 | JJ | denotes | insoluble |
| T21768 | 192-200 | NN | denotes | fraction |
| T21769 | 200-201 | . | denotes | . |
| T21770 | 202-205 | DT | denotes | The |
| T21771 | 206-213 | NN | denotes | protein |
| T21772 | 214-227 | NN | denotes | concentration |
| T21773 | 228-231 | VBD | denotes | was |
| T21774 | 232-242 | VBN | denotes | determined |
| T21775 | 243-245 | IN | denotes | by |
| T21776 | 246-249 | DT | denotes | the |
| T21777 | 250-253 | NNP | denotes | BCA |
| T21778 | 254-261 | NN | denotes | protein |
| T21779 | 262-267 | NN | denotes | assay |
| T21780 | 268-269 | -LRB- | denotes | ( |
| T21781 | 269-276 | NNP | denotes | Bio-Rad |
| T21782 | 276-277 | , | denotes | , |
| T21783 | 278-286 | NNP | denotes | Hercules |
| T21784 | 286-287 | , | denotes | , |
| T21785 | 288-290 | NNP | denotes | CA |
| T21786 | 290-291 | -RRB- | denotes | ) |
| T21787 | 291-292 | . | denotes | . |
| T21788 | 293-297 | NN | denotes | Cell |
| T21789 | 298-305 | NNS | denotes | lysates |
| T21790 | 306-310 | VBD | denotes | were |
| T21791 | 311-320 | VBN | denotes | separated |
| T21792 | 321-323 | IN | denotes | by |
| T21793 | 324-332 | NNP | denotes | SDS-PAGE |
| T21794 | 333-335 | IN | denotes | on |
| T21795 | 336-338 | CD | denotes | 10 |
| T21796 | 338-339 | NN | denotes | % |
| T21797 | 340-354 | NN | denotes | polyacrylamide |
| T21798 | 355-359 | NNS | denotes | gels |
| T21799 | 360-363 | CC | denotes | and |
| T21800 | 364-368 | RB | denotes | then |
| T21801 | 369-380 | VBD | denotes | transferred |
| T21802 | 381-385 | IN | denotes | onto |
| T21803 | 386-400 | JJ | denotes | nitrocellulose |
| T21804 | 401-410 | NNS | denotes | membranes |
| T21805 | 411-414 | CC | denotes | and |
| T21806 | 415-424 | VBN | denotes | subjected |
| T21807 | 425-427 | TO | denotes | to |
| T21808 | 428-435 | JJ | denotes | Western |
| T21809 | 436-444 | VBG | denotes | blotting |
| T21810 | 444-445 | . | denotes | . |
| T21811 | 446-449 | DT | denotes | The |
| T21812 | 450-463 | JJ | denotes | immunoblotted |
| T21813 | 464-472 | NNS | denotes | proteins |
| T21814 | 473-477 | VBD | denotes | were |
| T21815 | 478-486 | VBN | denotes | detected |
| T21816 | 487-489 | IN | denotes | by |
| T21817 | 490-493 | NNP | denotes | ECL |
| T21818 | 494-501 | NN | denotes | reagent |
| T21819 | 502-503 | -LRB- | denotes | ( |
| T21820 | 503-505 | NNP | denotes | GE |
| T21821 | 506-516 | NNP | denotes | Healthcare |
| T21822 | 517-529 | NNP | denotes | Bio-Sciences |
| T21823 | 530-535 | NNP | denotes | Corp. |
| T21824 | 535-536 | , | denotes | , |
| T21825 | 537-547 | NNP | denotes | Piscataway |
| T21826 | 547-548 | , | denotes | , |
| T21827 | 549-551 | NNP | denotes | NJ |
| T21828 | 551-552 | -RRB- | denotes | ) |
| T21829 | 552-553 | . | denotes | . |
| R16400 | T21735 | T21738 | amod | Western,Cells |
| R16401 | T21736 | T21738 | compound | Blot,Cells |
| R16402 | T21737 | T21738 | compound | Analysis,Cells |
| R16403 | T21738 | T21740 | nsubjpass | Cells,washed |
| R16404 | T21739 | T21740 | auxpass | were,washed |
| R16405 | T21740 | T21740 | ROOT | washed,washed |
| R16406 | T21741 | T21740 | prep | with,washed |
| R16407 | T21742 | T21741 | pobj | PBS,with |
| R16408 | T21743 | T21740 | cc | and,washed |
| R16409 | T21744 | T21740 | conj | resuspended,washed |
| R16410 | T21745 | T21744 | prep | in,resuspended |
| R16411 | T21746 | T21747 | compound | cell,lysis |
| R16412 | T21747 | T21748 | compound | lysis,buffer |
| R16413 | T21748 | T21745 | pobj | buffer,in |
| R16414 | T21749 | T21740 | punct | .,washed |
| R16415 | T21750 | T21753 | punct | (,Technology |
| R16416 | T21751 | T21753 | compound | Cell,Technology |
| R16417 | T21752 | T21753 | compound | Signaling,Technology |
| R16418 | T21753 | T21756 | nsubj | Technology,incubated |
| R16419 | T21754 | T21753 | punct | ),Technology |
| R16420 | T21755 | T21753 | cc | and,Technology |
| R16421 | T21756 | T21756 | ROOT | incubated,incubated |
| R16422 | T21757 | T21756 | prep | for,incubated |
| R16423 | T21758 | T21759 | nummod | 10,minutes |
| R16424 | T21759 | T21757 | pobj | minutes,for |
| R16425 | T21760 | T21759 | prep | on,minutes |
| R16426 | T21761 | T21760 | pobj | ice,on |
| R16427 | T21762 | T21763 | advmod | then,centrifuged |
| R16428 | T21763 | T21756 | conj | centrifuged,incubated |
| R16429 | T21764 | T21765 | aux | to,remove |
| R16430 | T21765 | T21763 | xcomp | remove,centrifuged |
| R16431 | T21766 | T21768 | det | the,fraction |
| R16432 | T21767 | T21768 | amod | insoluble,fraction |
| R16433 | T21768 | T21765 | dobj | fraction,remove |
| R16434 | T21769 | T21756 | punct | .,incubated |
| R16435 | T21770 | T21772 | det | The,concentration |
| R16436 | T21771 | T21772 | compound | protein,concentration |
| R16437 | T21772 | T21774 | nsubjpass | concentration,determined |
| R16438 | T21773 | T21774 | auxpass | was,determined |
| R16439 | T21774 | T21774 | ROOT | determined,determined |
| R16440 | T21775 | T21774 | agent | by,determined |
| R16441 | T21776 | T21779 | det | the,assay |
| R16442 | T21777 | T21779 | compound | BCA,assay |
| R16443 | T21778 | T21779 | compound | protein,assay |
| R16444 | T21779 | T21775 | pobj | assay,by |
| R16445 | T21780 | T21781 | punct | (,Bio-Rad |
| R16446 | T21781 | T21779 | appos | Bio-Rad,assay |
| R16447 | T21782 | T21781 | punct | ",",Bio-Rad |
| R16448 | T21783 | T21781 | conj | Hercules,Bio-Rad |
| R16449 | T21784 | T21783 | punct | ",",Hercules |
| R16450 | T21785 | T21783 | conj | CA,Hercules |
| R16451 | T21786 | T21781 | punct | ),Bio-Rad |
| R16452 | T21787 | T21774 | punct | .,determined |
| R16453 | T21788 | T21789 | compound | Cell,lysates |
| R16454 | T21789 | T21791 | nsubjpass | lysates,separated |
| R16455 | T21790 | T21791 | auxpass | were,separated |
| R16456 | T21791 | T21791 | ROOT | separated,separated |
| R16457 | T21792 | T21791 | agent | by,separated |
| R16458 | T21793 | T21792 | pobj | SDS-PAGE,by |
| R16459 | T21794 | T21791 | prep | on,separated |
| R16460 | T21795 | T21796 | nummod | 10,% |
| R16461 | T21796 | T21798 | nmod | %,gels |
| R16462 | T21797 | T21798 | compound | polyacrylamide,gels |
| R16463 | T21798 | T21794 | pobj | gels,on |
| R16464 | T21799 | T21791 | cc | and,separated |
| R16465 | T21800 | T21801 | advmod | then,transferred |
| R16466 | T21801 | T21791 | conj | transferred,separated |
| R16467 | T21802 | T21801 | prep | onto,transferred |
| R16468 | T21803 | T21804 | amod | nitrocellulose,membranes |
| R16469 | T21804 | T21802 | pobj | membranes,onto |
| R16470 | T21805 | T21801 | cc | and,transferred |
| R16471 | T21806 | T21801 | conj | subjected,transferred |
| R16472 | T21807 | T21806 | prep | to,subjected |
| R16473 | T21808 | T21809 | amod | Western,blotting |
| R16474 | T21809 | T21807 | pobj | blotting,to |
| R16475 | T21810 | T21791 | punct | .,separated |
| R16476 | T21811 | T21813 | det | The,proteins |
| R16477 | T21812 | T21813 | amod | immunoblotted,proteins |
| R16478 | T21813 | T21815 | nsubjpass | proteins,detected |
| R16479 | T21814 | T21815 | auxpass | were,detected |
| R16480 | T21815 | T21815 | ROOT | detected,detected |
| R16481 | T21816 | T21815 | agent | by,detected |
| R16482 | T21817 | T21818 | compound | ECL,reagent |
| R16483 | T21818 | T21816 | pobj | reagent,by |
| R16484 | T21819 | T21823 | punct | (,Corp. |
| R16485 | T21820 | T21823 | compound | GE,Corp. |
| R16486 | T21821 | T21823 | compound | Healthcare,Corp. |
| R16487 | T21822 | T21823 | compound | Bio-Sciences,Corp. |
| R16488 | T21823 | T21818 | appos | Corp.,reagent |
| R16489 | T21824 | T21823 | punct | ",",Corp. |
| R16490 | T21825 | T21823 | npadvmod | Piscataway,Corp. |
| R16491 | T21826 | T21825 | punct | ",",Piscataway |
| R16492 | T21827 | T21825 | conj | NJ,Piscataway |
| R16493 | T21828 | T21823 | punct | ),Corp. |
| R16494 | T21829 | T21815 | punct | .,detected |