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PMC:3245220 / 44679-45797 JSONTXT

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bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T21226 338-342 P17252 denotes PKCα
T21227 447-451 P17252 denotes PKCα
T21228 461-464 P21579 denotes p65
T21229 461-464 Q04206 denotes p65
T21230 821-826 P09603 denotes M-CSF
T21231 823-826 P04141 denotes CSF
T21232 954-963 P08758 denotes Annexin V
T21233 1112-1117 P09603 denotes M-CSF
T21234 1114-1117 P04141 denotes CSF

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T21235 338-348 Protein denotes PKCα siRNA
T21236 447-451 Protein denotes PKCα
T21237 461-464 Protein denotes p65
T21238 954-963 Protein denotes Annexin V
T21239 821-826 Protein denotes M-CSF
T21240 1112-1117 Protein denotes M-CSF

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T21292 338-365 Protein denotes PKCα siRNA or control siRNA
T21293 447-451 Protein denotes PKCα
T21294 455-475 Protein denotes NF-κB p65 constructs
T21295 821-826 Protein denotes M-CSF
T21296 915-919 Protein denotes SEAP
T21297 1112-1117 Protein denotes M-CSF

events-check-again

Id Subject Object Predicate Lexical cue
T21286 338-348 Protein denotes PKCα siRNA
T21287 447-451 Protein denotes PKCα
T21288 461-464 Protein denotes p65
T21289 821-826 Protein denotes M-CSF
T21290 954-963 Protein denotes Annexin V
T21291 1112-1117 Protein denotes M-CSF

GO-CC

Id Subject Object Predicate Lexical cue
T21252 288-293 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T21253 564-569 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T21254 717-722 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T21255 1015-1020 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

GO-MF

Id Subject Object Predicate Lexical cue
T21250 338-342 http://purl.obolibrary.org/obo/GO_0004697 denotes PKCα
T21251 447-451 http://purl.obolibrary.org/obo/GO_0004697 denotes PKCα

UBERON-AE

Id Subject Object Predicate Lexical cue
T20813 648-653 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum
T20814 783-788 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T21032 0-9 JJ denotes Transient
T21033 10-22 NN denotes Transfection
T21034 23-25 IN denotes of
T21035 26-33 JJ denotes Primary
T21036 34-39 JJ denotes Human
T21037 40-49 NN denotes Monocytes
T21038 50-59 NN denotes Monocytes
T21039 60-64 VB denotes were
T21040 65-76 VB denotes transfected
T21041 77-82 VB denotes using
T21042 83-86 DT denotes the
T21043 87-92 JJ denotes human
T21044 93-101 NN denotes monocyte
T21045 102-107 NNP denotes Amaxa
T21046 108-120 NN denotes nucleofector
T21047 121-124 NN denotes kit
T21048 125-126 -LRB- denotes (
T21049 126-131 NNP denotes Lonza
T21050 132-144 NNP denotes Walkersville
T21051 145-148 NNP denotes Inc
T21052 148-149 -COMMA- denotes ,
T21053 150-162 NNP denotes Walkersville
T21054 162-163 -COMMA- denotes ,
T21055 164-166 NNP denotes MD
T21056 166-167 -RRB- denotes )
T21057 168-170 IN denotes as
T21058 171-181 RB denotes previously
T21059 182-191 VB denotes described
T21060 192-193 -LRB- denotes [
T21061 193-195 CD denotes 51
T21062 195-196 -RRB- denotes ]
T21063 198-205 RB denotes Briefly
T21064 205-206 -COMMA- denotes ,
T21065 207-216 NN denotes monocytes
T21066 217-221 VB denotes were
T21067 222-233 VB denotes resuspended
T21068 234-236 IN denotes in
T21069 237-242 NNP denotes Amaxa
T21070 243-255 NNP denotes Nucleofactor
T21071 256-264 NN denotes solution
T21072 265-267 IN denotes at
T21073 268-269 DT denotes a
T21074 270-277 NN denotes density
T21075 278-280 IN denotes of
T21076 281-287 CD denotes 20×106
T21077 288-296 NN denotes cells/ml
T21078 296-297 -COMMA- denotes ,
T21079 298-301 CC denotes and
T21080 302-303 CD denotes 2
T21081 304-306 NN denotes µg
T21082 307-309 IN denotes of
T21083 310-315 JJ denotes total
T21084 316-323 NN denotes plasmid
T21085 324-327 NN denotes DNA
T21086 328-331 CD denotes 100
T21087 332-334 NN denotes nM
T21088 335-337 IN denotes of
T21089 338-342 NN denotes PKCα
T21090 343-348 NN denotes siRNA
T21091 349-351 CC denotes or
T21092 352-359 NN denotes control
T21093 360-365 NN denotes siRNA
T21094 366-369 VB denotes was
T21095 370-375 VB denotes added
T21096 376-379 CC denotes and
T21097 380-391 VB denotes transfected
T21098 392-397 VB denotes using
T21099 398-405 NN denotes program
T21100 406-411 NNP denotes Amaxa
T21101 412-416 NN denotes Y-01
T21102 418-421 IN denotes For
T21103 422-437 NN denotes co-transfection
T21104 438-445 NN denotes studies
T21105 445-446 -COMMA- denotes ,
T21106 447-451 NN denotes PKCα
T21107 452-454 CC denotes or
T21108 455-460 NN denotes NF-κB
T21109 461-464 NN denotes p65
T21110 465-475 NN denotes constructs
T21111 476-480 VB denotes were
T21112 481-486 VB denotes mixed
T21113 487-489 IN denotes at
T21114 490-491 DT denotes a
T21115 492-497 NN denotes ratio
T21116 498-500 IN denotes of
T21117 501-504 NN denotes 5∶1
T21118 505-509 IN denotes with
T21119 510-513 DT denotes the
T21120 514-522 NN denotes reporter
T21121 523-530 NN denotes plasmid
T21122 532-543 RB denotes Immediately
T21123 544-549 IN denotes after
T21124 550-562 NN denotes transfection
T21125 562-563 -COMMA- denotes ,
T21126 564-569 NN denotes cells
T21127 570-574 VB denotes were
T21128 575-581 VB denotes washed
T21129 582-586 IN denotes with
T21130 587-588 CD denotes 1
T21131 589-591 NN denotes ml
T21132 592-594 IN denotes of
T21133 595-599 NN denotes RPMI
T21134 600-606 NN denotes medium
T21135 607-617 VB denotes containing
T21136 618-619 CD denotes 2
T21137 620-622 NN denotes mM
T21138 623-632 NN denotes glutamine
T21139 633-636 CC denotes and
T21140 637-639 CD denotes 10
T21141 639-640 NN denotes %
T21142 641-644 NN denotes FBS
T21143 645-647 CC denotes or
T21144 648-653 NN denotes serum
T21145 654-658 JJ denotes free
T21146 659-662 NN denotes LGM
T21147 663-669 NN denotes medium
T21148 670-671 -LRB- denotes (
T21149 671-676 NNP denotes Lonza
T21150 677-689 NNP denotes Walkersville
T21151 690-694 NNP denotes Inc.
T21152 694-695 -RRB- denotes )
T21153 696-700 RB denotes then
T21154 701-707 VB denotes plated
T21155 708-710 IN denotes at
T21156 711-716 CD denotes 4×105
T21157 717-727 NN denotes cells/well
T21158 728-730 IN denotes in
T21159 731-738 JJ denotes 24-well
T21160 739-745 NN denotes plates
T21161 747-750 DT denotes The
T21162 751-759 JJ denotes original
T21163 760-766 NN denotes medium
T21164 767-770 VB denotes was
T21165 771-779 VB denotes replaced
T21166 780-782 IN denotes by
T21167 783-788 NN denotes serum
T21168 789-793 JJ denotes free
T21169 794-797 NN denotes LGM
T21170 798-804 NN denotes medium
T21171 805-812 IN denotes without
T21172 813-815 CC denotes or
T21173 816-820 IN denotes with
T21174 821-826 NN denotes M-CSF
T21175 827-828 -LRB- denotes (
T21176 828-831 CD denotes 100
T21177 832-837 NN denotes ng/ml
T21178 837-838 -RRB- denotes )
T21179 839-842 CD denotes one
T21180 843-847 NN denotes hour
T21181 848-853 RB denotes later
T21182 855-858 DT denotes The
T21183 859-863 JJ denotes next
T21184 864-867 NN denotes day
T21185 867-868 -COMMA- denotes ,
T21186 869-878 JJ denotes cell-free
T21187 879-891 NN denotes supernatants
T21188 892-896 VB denotes were
T21189 897-906 VB denotes collected
T21190 907-910 IN denotes for
T21191 911-914 DT denotes the
T21192 915-919 NN denotes SEAP
T21193 920-928 NN denotes analysis
T21194 930-935 NN denotes Cells
T21195 936-940 VB denotes were
T21196 941-948 VB denotes stained
T21197 949-953 IN denotes with
T21198 954-961 NN denotes Annexin
T21199 962-973 NN denotes V/propidium
T21200 974-980 NN denotes iodide
T21201 981-982 -LRB- denotes (
T21202 982-984 NN denotes PI
T21203 984-985 -RRB- denotes )
T21204 987-990 IN denotes For
T21205 991-994 DT denotes the
T21206 995-1005 NN denotes inhibition
T21207 1006-1013 NN denotes studies
T21208 1013-1014 -COMMA- denotes ,
T21209 1015-1020 NN denotes cells
T21210 1021-1025 VB denotes were
T21211 1026-1039 JJ denotes pre-incubated
T21212 1040-1044 IN denotes with
T21213 1045-1054 NN denotes inhibitor
T21214 1055-1058 IN denotes for
T21215 1059-1061 CD denotes 30
T21216 1062-1069 NN denotes minutes
T21217 1070-1072 IN denotes in
T21218 1073-1079 JJ denotes X-vivo
T21219 1080-1086 NN denotes medium
T21220 1087-1092 JJ denotes prior
T21221 1093-1095 TO denotes to
T21222 1096-1099 DT denotes the
T21223 1100-1108 NN denotes addition
T21224 1109-1111 IN denotes of
T21225 1112-1117 NN denotes M-CSF
R15863 T21033 T21032 arg1Of Transfection,Transient
R15864 T21033 T21034 arg1Of Transfection,of
R15865 T21037 T21034 arg2Of Monocytes,of
R15866 T21037 T21035 arg1Of Monocytes,Primary
R15867 T21037 T21036 arg1Of Monocytes,Human
R15868 T21038 T21039 arg1Of Monocytes,were
R15869 T21038 T21040 arg2Of Monocytes,transfected
R15870 T21040 T21039 arg2Of transfected,were
R15871 T21040 T21041 modOf transfected,using
R15872 T21047 T21041 arg2Of kit,using
R15873 T21047 T21042 arg1Of kit,the
R15874 T21047 T21043 arg1Of kit,human
R15875 T21047 T21044 arg1Of kit,monocyte
R15876 T21047 T21045 arg1Of kit,Amaxa
R15877 T21047 T21046 arg1Of kit,nucleofector
R15878 T21047 T21048 arg1Of kit,(
R15879 T21047 T21057 arg1Of kit,as
R15880 T21051 T21048 arg2Of Inc,(
R15881 T21051 T21049 arg1Of Inc,Lonza
R15882 T21051 T21050 arg1Of Inc,Walkersville
R15883 T21051 T21052 arg1Of Inc,","
R15884 T21053 T21052 arg2Of Walkersville,","
R15885 T21053 T21054 arg1Of Walkersville,","
R15886 T21055 T21054 arg2Of MD,","
R15887 T21056 T21048 arg3Of ),(
R15888 T21059 T21058 arg1Of described,previously
R15889 T21061 T21057 arg2Of 51,as
R15890 T21061 T21059 arg1Of 51,described
R15891 T21061 T21060 arg2Of 51,[
R15892 T21062 T21060 arg3Of ],[
R15893 T21065 T21066 arg1Of monocytes,were
R15894 T21065 T21067 arg2Of monocytes,resuspended
R15895 T21067 T21063 arg1Of resuspended,Briefly
R15896 T21067 T21064 arg1Of resuspended,","
R15897 T21067 T21066 arg2Of resuspended,were
R15898 T21067 T21068 arg1Of resuspended,in
R15899 T21067 T21072 arg1Of resuspended,at
R15900 T21067 T21079 arg1Of resuspended,and
R15901 T21070 T21069 arg1Of Nucleofactor,Amaxa
R15902 T21071 T21068 arg2Of solution,in
R15903 T21071 T21070 arg1Of solution,Nucleofactor
R15904 T21074 T21072 arg2Of density,at
R15905 T21074 T21073 arg1Of density,a
R15906 T21074 T21075 arg1Of density,of
R15907 T21077 T21075 arg2Of cells/ml,of
R15908 T21077 T21076 arg1Of cells/ml,20×106
R15909 T21079 T21078 arg1Of and,","
R15910 T21081 T21080 arg1Of µg,2
R15911 T21081 T21082 arg1Of µg,of
R15912 T21081 T21094 arg1Of µg,was
R15913 T21081 T21095 arg2Of µg,added
R15914 T21081 T21097 arg2Of µg,transfected
R15915 T21087 T21082 arg2Of nM,of
R15916 T21087 T21083 arg1Of nM,total
R15917 T21087 T21084 arg1Of nM,plasmid
R15918 T21087 T21085 arg1Of nM,DNA
R15919 T21087 T21086 arg1Of nM,100
R15920 T21087 T21088 arg1Of nM,of
R15921 T21090 T21089 arg1Of siRNA,PKCα
R15922 T21090 T21091 arg1Of siRNA,or
R15923 T21091 T21088 arg2Of or,of
R15924 T21093 T21091 arg2Of siRNA,or
R15925 T21093 T21092 arg1Of siRNA,control
R15926 T21095 T21096 arg1Of added,and
R15927 T21096 T21079 arg2Of and,and
R15928 T21096 T21094 arg2Of and,was
R15929 T21096 T21098 modOf and,using
R15930 T21097 T21096 arg2Of transfected,and
R15931 T21101 T21098 arg2Of Y-01,using
R15932 T21101 T21099 arg1Of Y-01,program
R15933 T21101 T21100 arg1Of Y-01,Amaxa
R15934 T21104 T21102 arg2Of studies,For
R15935 T21104 T21103 arg1Of studies,co-transfection
R15936 T21106 T21107 arg1Of PKCα,or
R15937 T21108 T21107 arg2Of NF-κB,or
R15938 T21110 T21106 arg1Of constructs,PKCα
R15939 T21110 T21108 arg1Of constructs,NF-κB
R15940 T21110 T21109 arg1Of constructs,p65
R15941 T21110 T21111 arg1Of constructs,were
R15942 T21110 T21112 arg2Of constructs,mixed
R15943 T21112 T21102 arg1Of mixed,For
R15944 T21112 T21105 arg1Of mixed,","
R15945 T21112 T21111 arg2Of mixed,were
R15946 T21112 T21113 arg1Of mixed,at
R15947 T21115 T21113 arg2Of ratio,at
R15948 T21115 T21114 arg1Of ratio,a
R15949 T21115 T21116 arg1Of ratio,of
R15950 T21115 T21118 arg1Of ratio,with
R15951 T21117 T21116 arg2Of 5∶1,of
R15952 T21121 T21118 arg2Of plasmid,with
R15953 T21121 T21119 arg1Of plasmid,the
R15954 T21121 T21120 arg1Of plasmid,reporter
R15955 T21123 T21122 arg1Of after,Immediately
R15956 T21124 T21123 arg2Of transfection,after
R15957 T21126 T21127 arg1Of cells,were
R15958 T21126 T21128 arg2Of cells,washed
R15959 T21128 T21123 arg1Of washed,after
R15960 T21128 T21125 arg1Of washed,","
R15961 T21128 T21127 arg2Of washed,were
R15962 T21128 T21129 arg1Of washed,with
R15963 T21131 T21129 arg2Of ml,with
R15964 T21131 T21130 arg1Of ml,1
R15965 T21131 T21132 arg1Of ml,of
R15966 T21134 T21132 arg2Of medium,of
R15967 T21134 T21133 arg1Of medium,RPMI
R15968 T21134 T21135 arg1Of medium,containing
R15969 T21136 T21137 arg1Of 2,mM
R15970 T21138 T21136 arg1Of glutamine,2
R15971 T21138 T21139 arg1Of glutamine,and
R15972 T21139 T21143 arg1Of and,or
R15973 T21140 T21141 arg1Of 10,%
R15974 T21142 T21139 arg2Of FBS,and
R15975 T21142 T21140 arg1Of FBS,10
R15976 T21143 T21135 arg2Of or,containing
R15977 T21143 T21154 arg2Of or,plated
R15978 T21147 T21143 arg2Of medium,or
R15979 T21147 T21144 arg1Of medium,serum
R15980 T21147 T21145 arg1Of medium,free
R15981 T21147 T21146 arg1Of medium,LGM
R15982 T21147 T21148 arg1Of medium,(
R15983 T21151 T21148 arg2Of Inc.,(
R15984 T21151 T21149 arg1Of Inc.,Lonza
R15985 T21151 T21150 arg1Of Inc.,Walkersville
R15986 T21152 T21148 arg3Of ),(
R15987 T21154 T21153 arg1Of plated,then
R15988 T21154 T21155 arg1Of plated,at
R15989 T21154 T21158 arg1Of plated,in
R15990 T21157 T21155 arg2Of cells/well,at
R15991 T21157 T21156 arg1Of cells/well,4×105
R15992 T21160 T21158 arg2Of plates,in
R15993 T21160 T21159 arg1Of plates,24-well
R15994 T21163 T21161 arg1Of medium,The
R15995 T21163 T21162 arg1Of medium,original
R15996 T21163 T21164 arg1Of medium,was
R15997 T21163 T21165 arg2Of medium,replaced
R15998 T21165 T21164 arg2Of replaced,was
R15999 T21165 T21171 arg1Of replaced,without
R16000 T21165 T21173 arg1Of replaced,with
R16001 T21170 T21165 arg1Of medium,replaced
R16002 T21170 T21166 arg2Of medium,by
R16003 T21170 T21167 arg1Of medium,serum
R16004 T21170 T21168 arg1Of medium,free
R16005 T21170 T21169 arg1Of medium,LGM
R16006 T21171 T21172 arg1Of without,or
R16007 T21173 T21172 arg2Of with,or
R16008 T21174 T21171 arg2Of M-CSF,without
R16009 T21174 T21173 arg2Of M-CSF,with
R16010 T21174 T21175 arg1Of M-CSF,(
R16011 T21174 T21181 arg1Of M-CSF,later
R16012 T21177 T21175 arg2Of ng/ml,(
R16013 T21177 T21176 arg1Of ng/ml,100
R16014 T21178 T21175 arg3Of ),(
R16015 T21180 T21179 arg1Of hour,one
R16016 T21181 T21180 arg1Of later,hour
R16017 T21184 T21182 arg1Of day,The
R16018 T21184 T21183 arg1Of day,next
R16019 T21187 T21186 arg1Of supernatants,cell-free
R16020 T21187 T21188 arg1Of supernatants,were
R16021 T21187 T21189 arg2Of supernatants,collected
R16022 T21189 T21184 arg1Of collected,day
R16023 T21189 T21185 arg1Of collected,","
R16024 T21189 T21188 arg2Of collected,were
R16025 T21189 T21190 arg1Of collected,for
R16026 T21193 T21190 arg2Of analysis,for
R16027 T21193 T21191 arg1Of analysis,the
R16028 T21193 T21192 arg1Of analysis,SEAP
R16029 T21194 T21195 arg1Of Cells,were
R16030 T21194 T21196 arg2Of Cells,stained
R16031 T21196 T21195 arg2Of stained,were
R16032 T21196 T21197 arg1Of stained,with
R16033 T21200 T21197 arg2Of iodide,with
R16034 T21200 T21198 arg1Of iodide,Annexin
R16035 T21200 T21199 arg1Of iodide,V/propidium
R16036 T21200 T21201 arg1Of iodide,(
R16037 T21202 T21201 arg2Of PI,(
R16038 T21203 T21201 arg3Of ),(
R16039 T21207 T21204 arg2Of studies,For
R16040 T21207 T21205 arg1Of studies,the
R16041 T21207 T21206 arg1Of studies,inhibition
R16042 T21209 T21210 arg1Of cells,were
R16043 T21209 T21211 arg1Of cells,pre-incubated
R16044 T21210 T21204 arg1Of were,For
R16045 T21210 T21208 arg1Of were,","
R16046 T21211 T21210 arg2Of pre-incubated,were
R16047 T21211 T21212 arg1Of pre-incubated,with
R16048 T21213 T21212 arg2Of inhibitor,with
R16049 T21213 T21214 arg1Of inhibitor,for
R16050 T21216 T21214 arg2Of minutes,for
R16051 T21216 T21215 arg1Of minutes,30
R16052 T21216 T21217 arg1Of minutes,in
R16053 T21219 T21217 arg2Of medium,in
R16054 T21219 T21218 arg1Of medium,X-vivo
R16055 T21219 T21220 arg1Of medium,prior
R16056 T21220 T21221 arg1Of prior,to
R16057 T21223 T21221 arg2Of addition,to
R16058 T21223 T21222 arg1Of addition,the
R16059 T21223 T21224 arg1Of addition,of
R16060 T21225 T21224 arg2Of M-CSF,of

sentences

Id Subject Object Predicate Lexical cue
T20815 0-49 Sentence denotes Transient Transfection of Primary Human Monocytes
T20816 50-197 Sentence denotes Monocytes were transfected using the human monocyte Amaxa nucleofector kit (Lonza Walkersville Inc, Walkersville, MD) as previously described [51].
T20817 198-417 Sentence denotes Briefly, monocytes were resuspended in Amaxa Nucleofactor solution at a density of 20×106 cells/ml, and 2 µg of total plasmid DNA 100 nM of PKCα siRNA or control siRNA was added and transfected using program Amaxa Y-01.
T20818 418-531 Sentence denotes For co-transfection studies, PKCα or NF-κB p65 constructs were mixed at a ratio of 5∶1 with the reporter plasmid.
T20819 532-746 Sentence denotes Immediately after transfection, cells were washed with 1 ml of RPMI medium containing 2 mM glutamine and 10% FBS or serum free LGM medium (Lonza Walkersville Inc.) then plated at 4×105 cells/well in 24-well plates.
T20820 747-854 Sentence denotes The original medium was replaced by serum free LGM medium without or with M-CSF (100 ng/ml) one hour later.
T20821 855-929 Sentence denotes The next day, cell-free supernatants were collected for the SEAP analysis.
T20822 930-986 Sentence denotes Cells were stained with Annexin V/propidium iodide (PI).
T20823 987-1118 Sentence denotes For the inhibition studies, cells were pre-incubated with inhibitor for 30 minutes in X-vivo medium prior to the addition of M-CSF.
T310 0-49 Sentence denotes Transient Transfection of Primary Human Monocytes
T311 50-197 Sentence denotes Monocytes were transfected using the human monocyte Amaxa nucleofector kit (Lonza Walkersville Inc, Walkersville, MD) as previously described [51].
T312 198-417 Sentence denotes Briefly, monocytes were resuspended in Amaxa Nucleofactor solution at a density of 20×106 cells/ml, and 2 µg of total plasmid DNA 100 nM of PKCα siRNA or control siRNA was added and transfected using program Amaxa Y-01.
T313 418-531 Sentence denotes For co-transfection studies, PKCα or NF-κB p65 constructs were mixed at a ratio of 5∶1 with the reporter plasmid.
T314 532-746 Sentence denotes Immediately after transfection, cells were washed with 1 ml of RPMI medium containing 2 mM glutamine and 10% FBS or serum free LGM medium (Lonza Walkersville Inc.) then plated at 4×105 cells/well in 24-well plates.
T315 747-854 Sentence denotes The original medium was replaced by serum free LGM medium without or with M-CSF (100 ng/ml) one hour later.
T316 855-929 Sentence denotes The next day, cell-free supernatants were collected for the SEAP analysis.
T317 930-986 Sentence denotes Cells were stained with Annexin V/propidium iodide (PI).
T318 987-1118 Sentence denotes For the inhibition studies, cells were pre-incubated with inhibitor for 30 minutes in X-vivo medium prior to the addition of M-CSF.

2_test

Id Subject Object Predicate Lexical cue
22216091-16931806-90609745 193-195 16931806 denotes 51

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T20824 0-9 NNP denotes Transient
T20825 10-22 NNP denotes Transfection
T20826 23-25 IN denotes of
T20827 26-33 JJ denotes Primary
T20828 34-39 JJ denotes Human
T20829 40-49 NNP denotes Monocytes
T20830 50-59 NNPS denotes Monocytes
T20831 60-64 VBD denotes were
T20832 65-76 VBN denotes transfected
T20833 77-82 VBG denotes using
T20834 83-86 DT denotes the
T20835 87-92 JJ denotes human
T20836 93-101 NN denotes monocyte
T20837 102-107 NNP denotes Amaxa
T20838 108-120 NN denotes nucleofector
T20839 121-124 NN denotes kit
T20840 125-126 -LRB- denotes (
T20841 126-131 NNP denotes Lonza
T20842 132-144 NNP denotes Walkersville
T20843 145-148 NNP denotes Inc
T20844 148-149 , denotes ,
T20845 150-162 NNP denotes Walkersville
T20846 162-163 , denotes ,
T20847 164-166 NNP denotes MD
T20848 166-167 -RRB- denotes )
T20849 168-170 IN denotes as
T20850 171-181 RB denotes previously
T20851 182-191 VBN denotes described
T20852 192-193 NNP denotes [
T20853 193-195 CD denotes 51
T20854 195-196 NNP denotes ]
T20855 196-197 . denotes .
T20856 198-205 RB denotes Briefly
T20857 205-206 , denotes ,
T20858 207-216 NNS denotes monocytes
T20859 217-221 VBD denotes were
T20860 222-233 VBN denotes resuspended
T20861 234-236 IN denotes in
T20862 237-242 NNP denotes Amaxa
T20863 243-255 NNP denotes Nucleofactor
T20864 256-264 NN denotes solution
T20865 265-267 IN denotes at
T20866 268-269 DT denotes a
T20867 270-277 NN denotes density
T20868 278-280 IN denotes of
T20869 281-283 CD denotes 20
T20870 283-284 CD denotes ×
T20871 284-287 CD denotes 106
T20872 288-296 NN denotes cells/ml
T20873 296-297 , denotes ,
T20874 298-301 CC denotes and
T20875 302-303 CD denotes 2
T20876 304-306 NN denotes µg
T20877 307-309 IN denotes of
T20878 310-315 JJ denotes total
T20879 316-323 JJ denotes plasmid
T20880 324-327 NN denotes DNA
T20881 328-331 CD denotes 100
T20882 332-334 NNP denotes nM
T20883 335-337 IN denotes of
T20884 338-342 NNP denotes PKCα
T20885 343-348 NNP denotes siRNA
T20886 349-351 CC denotes or
T20887 352-359 VB denotes control
T20888 360-365 NNP denotes siRNA
T20889 366-369 VBD denotes was
T20890 370-375 VBN denotes added
T20891 376-379 CC denotes and
T20892 380-391 VBN denotes transfected
T20893 392-397 VBG denotes using
T20894 398-405 NN denotes program
T20895 406-411 NNP denotes Amaxa
T20896 412-416 NNP denotes Y-01
T20897 416-417 . denotes .
T20898 418-421 IN denotes For
T20899 422-437 JJ denotes co-transfection
T20900 438-445 NNS denotes studies
T20901 445-446 , denotes ,
T20902 447-451 NNP denotes PKCα
T20903 452-454 CC denotes or
T20904 455-460 NNP denotes NF-κB
T20905 461-464 CD denotes p65
T20906 465-475 NNS denotes constructs
T20907 476-480 VBD denotes were
T20908 481-486 VBN denotes mixed
T20909 487-489 IN denotes at
T20910 490-491 DT denotes a
T20911 492-497 NN denotes ratio
T20912 498-500 IN denotes of
T20913 501-502 CD denotes 5
T20914 502-503 CD denotes ∶
T20915 503-504 CD denotes 1
T20916 505-509 IN denotes with
T20917 510-513 DT denotes the
T20918 514-522 NN denotes reporter
T20919 523-530 NN denotes plasmid
T20920 530-531 . denotes .
T20921 532-543 RB denotes Immediately
T20922 544-549 IN denotes after
T20923 550-562 NN denotes transfection
T20924 562-563 , denotes ,
T20925 564-569 NNS denotes cells
T20926 570-574 VBD denotes were
T20927 575-581 VBN denotes washed
T20928 582-586 IN denotes with
T20929 587-588 CD denotes 1
T20930 589-591 NN denotes ml
T20931 592-594 IN denotes of
T20932 595-599 NNP denotes RPMI
T20933 600-606 NN denotes medium
T20934 607-617 VBG denotes containing
T20935 618-619 CD denotes 2
T20936 620-622 NNP denotes mM
T20937 623-632 NN denotes glutamine
T20938 633-636 CC denotes and
T20939 637-639 CD denotes 10
T20940 639-640 NN denotes %
T20941 641-644 NNP denotes FBS
T20942 645-647 CC denotes or
T20943 648-653 NN denotes serum
T20944 654-658 JJ denotes free
T20945 659-662 NNP denotes LGM
T20946 663-669 NN denotes medium
T20947 670-671 -LRB- denotes (
T20948 671-676 NNP denotes Lonza
T20949 677-689 NNP denotes Walkersville
T20950 690-694 NNP denotes Inc.
T20951 694-695 -RRB- denotes )
T20952 696-700 RB denotes then
T20953 701-707 VBD denotes plated
T20954 708-710 IN denotes at
T20955 711-712 CD denotes 4
T20956 712-713 CD denotes ×
T20957 713-716 CD denotes 105
T20958 717-727 NN denotes cells/well
T20959 728-730 IN denotes in
T20960 731-738 JJ denotes 24-well
T20961 739-745 NNS denotes plates
T20962 745-746 . denotes .
T20963 747-750 DT denotes The
T20964 751-759 JJ denotes original
T20965 760-766 NN denotes medium
T20966 767-770 VBD denotes was
T20967 771-779 VBN denotes replaced
T20968 780-782 IN denotes by
T20969 783-788 NN denotes serum
T20970 789-793 JJ denotes free
T20971 794-797 NNP denotes LGM
T20972 798-804 NN denotes medium
T20973 805-812 IN denotes without
T20974 813-815 CC denotes or
T20975 816-820 IN denotes with
T20976 821-826 NNP denotes M-CSF
T20977 827-828 -LRB- denotes (
T20978 828-831 CD denotes 100
T20979 832-837 NN denotes ng/ml
T20980 837-838 -RRB- denotes )
T20981 839-842 CD denotes one
T20982 843-847 NN denotes hour
T20983 848-853 RB denotes later
T20984 853-854 . denotes .
T20985 855-858 DT denotes The
T20986 859-863 JJ denotes next
T20987 864-867 NN denotes day
T20988 867-868 , denotes ,
T20989 869-878 JJ denotes cell-free
T20990 879-891 NNS denotes supernatants
T20991 892-896 VBD denotes were
T20992 897-906 VBN denotes collected
T20993 907-910 IN denotes for
T20994 911-914 DT denotes the
T20995 915-919 NNP denotes SEAP
T20996 920-928 NN denotes analysis
T20997 928-929 . denotes .
T20998 930-935 NNS denotes Cells
T20999 936-940 VBD denotes were
T21000 941-948 VBN denotes stained
T21001 949-953 IN denotes with
T21002 954-961 NNP denotes Annexin
T21003 962-973 NNP denotes V/propidium
T21004 974-980 NN denotes iodide
T21005 981-982 -LRB- denotes (
T21006 982-984 NNP denotes PI
T21007 984-985 -RRB- denotes )
T21008 985-986 . denotes .
T21009 987-990 IN denotes For
T21010 991-994 DT denotes the
T21011 995-1005 NN denotes inhibition
T21012 1006-1013 NNS denotes studies
T21013 1013-1014 , denotes ,
T21014 1015-1020 NNS denotes cells
T21015 1021-1025 VBD denotes were
T21016 1026-1039 VBN denotes pre-incubated
T21017 1040-1044 IN denotes with
T21018 1045-1054 NN denotes inhibitor
T21019 1055-1058 IN denotes for
T21020 1059-1061 CD denotes 30
T21021 1062-1069 NNS denotes minutes
T21022 1070-1072 IN denotes in
T21023 1073-1079 NNP denotes X-vivo
T21024 1080-1086 NN denotes medium
T21025 1087-1092 RB denotes prior
T21026 1093-1095 TO denotes to
T21027 1096-1099 DT denotes the
T21028 1100-1108 NN denotes addition
T21029 1109-1111 IN denotes of
T21030 1112-1117 NNP denotes M-CSF
T21031 1117-1118 . denotes .
R15655 T20824 T20825 compound Transient,Transfection
R15656 T20825 T20832 nsubjpass Transfection,transfected
R15657 T20826 T20825 prep of,Transfection
R15658 T20827 T20828 compound Primary,Human
R15659 T20828 T20829 compound Human,Monocytes
R15660 T20829 T20830 compound Monocytes,Monocytes
R15661 T20830 T20826 pobj Monocytes,of
R15662 T20831 T20832 auxpass were,transfected
R15663 T20832 T20832 ROOT transfected,transfected
R15664 T20833 T20832 advcl using,transfected
R15665 T20834 T20839 det the,kit
R15666 T20835 T20836 amod human,monocyte
R15667 T20836 T20839 compound monocyte,kit
R15668 T20837 T20839 compound Amaxa,kit
R15669 T20838 T20839 compound nucleofector,kit
R15670 T20839 T20833 dobj kit,using
R15671 T20840 T20839 punct (,kit
R15672 T20841 T20843 compound Lonza,Inc
R15673 T20842 T20843 compound Walkersville,Inc
R15674 T20843 T20839 appos Inc,kit
R15675 T20844 T20843 punct ",",Inc
R15676 T20845 T20843 conj Walkersville,Inc
R15677 T20846 T20845 punct ",",Walkersville
R15678 T20847 T20845 appos MD,Walkersville
R15679 T20848 T20845 punct ),Walkersville
R15680 T20849 T20851 mark as,described
R15681 T20850 T20851 advmod previously,described
R15682 T20851 T20833 advcl described,using
R15683 T20852 T20854 nmod [,]
R15684 T20853 T20854 nummod 51,]
R15685 T20854 T20851 dobj ],described
R15686 T20855 T20832 punct .,transfected
R15687 T20856 T20860 advmod Briefly,resuspended
R15688 T20857 T20860 punct ",",resuspended
R15689 T20858 T20860 nsubjpass monocytes,resuspended
R15690 T20859 T20860 auxpass were,resuspended
R15691 T20860 T20860 ROOT resuspended,resuspended
R15692 T20861 T20860 prep in,resuspended
R15693 T20862 T20863 compound Amaxa,Nucleofactor
R15694 T20863 T20864 compound Nucleofactor,solution
R15695 T20864 T20861 pobj solution,in
R15696 T20865 T20864 prep at,solution
R15697 T20866 T20867 det a,density
R15698 T20867 T20865 pobj density,at
R15699 T20868 T20867 prep of,density
R15700 T20869 T20872 nummod 20,cells/ml
R15701 T20870 T20872 nummod ×,cells/ml
R15702 T20871 T20872 nummod 106,cells/ml
R15703 T20872 T20868 pobj cells/ml,of
R15704 T20873 T20860 punct ",",resuspended
R15705 T20874 T20860 cc and,resuspended
R15706 T20875 T20876 nummod 2,µg
R15707 T20876 T20890 nsubjpass µg,added
R15708 T20877 T20876 prep of,µg
R15709 T20878 T20880 amod total,DNA
R15710 T20879 T20880 compound plasmid,DNA
R15711 T20880 T20877 pobj DNA,of
R15712 T20881 T20882 nummod 100,nM
R15713 T20882 T20876 conj nM,µg
R15714 T20883 T20882 prep of,nM
R15715 T20884 T20885 compound PKCα,siRNA
R15716 T20885 T20883 pobj siRNA,of
R15717 T20886 T20882 cc or,nM
R15718 T20887 T20888 compound control,siRNA
R15719 T20888 T20882 conj siRNA,nM
R15720 T20889 T20890 auxpass was,added
R15721 T20890 T20860 conj added,resuspended
R15722 T20891 T20890 cc and,added
R15723 T20892 T20890 conj transfected,added
R15724 T20893 T20892 advcl using,transfected
R15725 T20894 T20896 compound program,Y-01
R15726 T20895 T20896 compound Amaxa,Y-01
R15727 T20896 T20893 dobj Y-01,using
R15728 T20897 T20890 punct .,added
R15729 T20898 T20907 prep For,were
R15730 T20899 T20900 amod co-transfection,studies
R15731 T20900 T20898 pobj studies,For
R15732 T20901 T20907 punct ",",were
R15733 T20902 T20906 nmod PKCα,constructs
R15734 T20903 T20902 cc or,PKCα
R15735 T20904 T20902 conj NF-κB,PKCα
R15736 T20905 T20904 nummod p65,NF-κB
R15737 T20906 T20907 nsubj constructs,were
R15738 T20907 T20907 ROOT were,were
R15739 T20908 T20907 acomp mixed,were
R15740 T20909 T20908 prep at,mixed
R15741 T20910 T20911 det a,ratio
R15742 T20911 T20909 pobj ratio,at
R15743 T20912 T20911 prep of,ratio
R15744 T20913 T20915 nummod 5,1
R15745 T20914 T20915 nummod ∶,1
R15746 T20915 T20912 pobj 1,of
R15747 T20916 T20908 prep with,mixed
R15748 T20917 T20919 det the,plasmid
R15749 T20918 T20919 compound reporter,plasmid
R15750 T20919 T20916 pobj plasmid,with
R15751 T20920 T20907 punct .,were
R15752 T20921 T20922 advmod Immediately,after
R15753 T20922 T20927 prep after,washed
R15754 T20923 T20922 pobj transfection,after
R15755 T20924 T20927 punct ",",washed
R15756 T20925 T20927 nsubjpass cells,washed
R15757 T20926 T20927 auxpass were,washed
R15758 T20927 T20927 ROOT washed,washed
R15759 T20928 T20927 prep with,washed
R15760 T20929 T20930 nummod 1,ml
R15761 T20930 T20928 pobj ml,with
R15762 T20931 T20930 prep of,ml
R15763 T20932 T20933 compound RPMI,medium
R15764 T20933 T20931 pobj medium,of
R15765 T20934 T20930 acl containing,ml
R15766 T20935 T20937 nummod 2,glutamine
R15767 T20936 T20937 compound mM,glutamine
R15768 T20937 T20934 dobj glutamine,containing
R15769 T20938 T20937 cc and,glutamine
R15770 T20939 T20940 nummod 10,%
R15771 T20940 T20941 compound %,FBS
R15772 T20941 T20937 conj FBS,glutamine
R15773 T20942 T20941 cc or,FBS
R15774 T20943 T20944 npadvmod serum,free
R15775 T20944 T20946 amod free,medium
R15776 T20945 T20946 compound LGM,medium
R15777 T20946 T20941 conj medium,FBS
R15778 T20947 T20950 punct (,Inc.
R15779 T20948 T20950 compound Lonza,Inc.
R15780 T20949 T20950 compound Walkersville,Inc.
R15781 T20950 T20946 appos Inc.,medium
R15782 T20951 T20950 punct ),Inc.
R15783 T20952 T20953 advmod then,plated
R15784 T20953 T20927 dep plated,washed
R15785 T20954 T20953 prep at,plated
R15786 T20955 T20958 nummod 4,cells/well
R15787 T20956 T20958 nummod ×,cells/well
R15788 T20957 T20958 nummod 105,cells/well
R15789 T20958 T20954 pobj cells/well,at
R15790 T20959 T20958 prep in,cells/well
R15791 T20960 T20961 amod 24-well,plates
R15792 T20961 T20959 pobj plates,in
R15793 T20962 T20927 punct .,washed
R15794 T20963 T20965 det The,medium
R15795 T20964 T20965 amod original,medium
R15796 T20965 T20967 nsubjpass medium,replaced
R15797 T20966 T20967 auxpass was,replaced
R15798 T20967 T20967 ROOT replaced,replaced
R15799 T20968 T20967 agent by,replaced
R15800 T20969 T20970 npadvmod serum,free
R15801 T20970 T20972 amod free,medium
R15802 T20971 T20972 compound LGM,medium
R15803 T20972 T20968 pobj medium,by
R15804 T20973 T20967 prep without,replaced
R15805 T20974 T20973 cc or,without
R15806 T20975 T20973 conj with,without
R15807 T20976 T20975 pobj M-CSF,with
R15808 T20977 T20976 punct (,M-CSF
R15809 T20978 T20979 nummod 100,ng/ml
R15810 T20979 T20976 appos ng/ml,M-CSF
R15811 T20980 T20979 punct ),ng/ml
R15812 T20981 T20982 nummod one,hour
R15813 T20982 T20983 npadvmod hour,later
R15814 T20983 T20967 advmod later,replaced
R15815 T20984 T20967 punct .,replaced
R15816 T20985 T20987 det The,day
R15817 T20986 T20987 amod next,day
R15818 T20987 T20992 npadvmod day,collected
R15819 T20988 T20992 punct ",",collected
R15820 T20989 T20990 amod cell-free,supernatants
R15821 T20990 T20992 nsubjpass supernatants,collected
R15822 T20991 T20992 auxpass were,collected
R15823 T20992 T20992 ROOT collected,collected
R15824 T20993 T20992 prep for,collected
R15825 T20994 T20996 det the,analysis
R15826 T20995 T20996 compound SEAP,analysis
R15827 T20996 T20993 pobj analysis,for
R15828 T20997 T20992 punct .,collected
R15829 T20998 T21000 nsubjpass Cells,stained
R15830 T20999 T21000 auxpass were,stained
R15831 T21000 T21000 ROOT stained,stained
R15832 T21001 T21000 prep with,stained
R15833 T21002 T21004 compound Annexin,iodide
R15834 T21003 T21004 compound V/propidium,iodide
R15835 T21004 T21001 pobj iodide,with
R15836 T21005 T21006 punct (,PI
R15837 T21006 T21004 appos PI,iodide
R15838 T21007 T21004 punct ),iodide
R15839 T21008 T21000 punct .,stained
R15840 T21009 T21016 prep For,pre-incubated
R15841 T21010 T21012 det the,studies
R15842 T21011 T21012 compound inhibition,studies
R15843 T21012 T21009 pobj studies,For
R15844 T21013 T21016 punct ",",pre-incubated
R15845 T21014 T21016 nsubjpass cells,pre-incubated
R15846 T21015 T21016 auxpass were,pre-incubated
R15847 T21016 T21016 ROOT pre-incubated,pre-incubated
R15848 T21017 T21016 prep with,pre-incubated
R15849 T21018 T21017 pobj inhibitor,with
R15850 T21019 T21016 prep for,pre-incubated
R15851 T21020 T21021 nummod 30,minutes
R15852 T21021 T21019 pobj minutes,for
R15853 T21022 T21021 prep in,minutes
R15854 T21023 T21024 compound X-vivo,medium
R15855 T21024 T21022 pobj medium,in
R15856 T21025 T21016 advmod prior,pre-incubated
R15857 T21026 T21025 prep to,prior
R15858 T21027 T21028 det the,addition
R15859 T21028 T21026 pobj addition,to
R15860 T21029 T21028 prep of,addition
R15861 T21030 T21029 pobj M-CSF,of
R15862 T21031 T21016 punct .,pre-incubated

test2

Id Subject Object Predicate Lexical cue
T20807 338-348 Protein denotes PKCα siRNA
T20808 447-451 Protein denotes PKCα
T20809 461-464 Protein denotes p65
T20810 821-826 Protein denotes M-CSF
T20811 954-963 Protein denotes Annexin V
T20812 1112-1117 Protein denotes M-CSF