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PMC:3245220 / 43655-44677 JSONTXT

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bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T20704 409-413 P17252 denotes PKCα
T20705 423-426 Q04206 denotes p65
T20706 423-426 P21579 denotes p65
T20707 528-532 P17252 denotes PKCα
T20708 732-737 P09603 denotes M-CSF
T20709 734-737 P04141 denotes CSF
T20710 900-905 P09603 denotes M-CSF
T20711 902-905 P04141 denotes CSF
T20712 988-991 Q9U6Y4 denotes GFP

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T20713 384-399 Gene_expression denotes co-transfection
T20714 384-399 Gene_expression denotes co-transfection
T20715 992-1002 Gene_expression denotes expression
T20716 409-413 Protein denotes PKCα
T20717 423-426 Protein denotes p65
T20718 528-538 Protein denotes PKCα siRNA
T20719 988-991 Protein denotes GFP
T20720 732-737 Protein denotes M-CSF
T20721 900-905 Protein denotes M-CSF
R15635 T20716 T20713 themeOf PKCα,co-transfection
R15636 T20717 T20714 themeOf p65,co-transfection
R15637 T20719 T20715 themeOf GFP,expression

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T20789 91-95 Protein denotes RPMI
T20790 152-181 Protein denotes Secreted alkaline phosphatase
T20791 183-187 Protein denotes SEAP
T20792 220-226 Protein denotes pNF-κB
T20793 227-231 Protein denotes SEAP
T20794 409-413 Protein denotes PKCα
T20795 417-437 Protein denotes NF-κB p65 constructs
T20796 528-555 Protein denotes PKCα siRNA or control siRNA
T20797 798-802 Protein denotes SEAP
T20798 900-905 Protein denotes M-CSF
T20799 988-991 Protein denotes GFP
T20800 992-1002 Gene_expression denotes expression
R15654 T20799 T20800 themeOf GFP,expression

events-check-again

Id Subject Object Predicate Lexical cue
T20780 384-399 Gene_expression denotes co-transfection
T20781 384-399 Gene_expression denotes co-transfection
T20782 409-413 Protein denotes PKCα
T20783 423-426 Protein denotes p65
T20784 528-538 Protein denotes PKCα siRNA
T20785 732-737 Protein denotes M-CSF
T20786 900-905 Protein denotes M-CSF
T20787 988-991 Protein denotes GFP
T20788 992-1002 Gene_expression denotes expression
R15651 T20782 T20780 themeOf PKCα,co-transfection
R15652 T20783 T20781 themeOf p65,co-transfection
R15653 T20787 T20788 themeOf GFP,expression

GO-CC

Id Subject Object Predicate Lexical cue
T20734 52-57 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T20735 254-259 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T20736 837-842 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T20737 1016-1021 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

GO-MF

Id Subject Object Predicate Lexical cue
T20730 170-181 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T20731 334-336 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T20732 409-413 http://purl.obolibrary.org/obo/GO_0004697 denotes PKCα
T20733 528-532 http://purl.obolibrary.org/obo/GO_0004697 denotes PKCα

GO-BP

Id Subject Object Predicate Lexical cue
T20801 170-181 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T20802 334-336 http://purl.obolibrary.org/obo/GO_0033968 denotes CA

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T20532 0-9 JJ denotes Transient
T20533 10-22 NN denotes Transfection
T20534 23-25 IN denotes of
T20535 26-29 NN denotes RAW
T20536 30-35 CD denotes 264.7
T20537 36-41 NN denotes Cells
T20538 42-45 NN denotes RAW
T20539 46-51 CD denotes 264.7
T20540 52-57 NN denotes cells
T20541 58-62 VB denotes were
T20542 63-69 VB denotes seeded
T20543 70-72 IN denotes in
T20544 73-80 JJ denotes 12-well
T20545 81-87 NN denotes plates
T20546 88-90 IN denotes in
T20547 91-95 NN denotes RPMI
T20548 96-102 NN denotes medium
T20549 103-113 VB denotes containing
T20550 114-115 CD denotes 5
T20551 115-116 NN denotes %
T20552 117-120 NNP denotes FBS
T20553 121-124 CD denotes one
T20554 125-128 NN denotes day
T20555 129-134 JJ denotes prior
T20556 135-137 TO denotes to
T20557 138-150 NN denotes transfection
T20558 150-151 -COMMA- denotes ,
T20559 152-160 VB denotes Secreted
T20560 161-169 NN denotes alkaline
T20561 170-181 NN denotes phosphatase
T20562 182-183 -LRB- denotes (
T20563 183-187 NN denotes SEAP
T20564 187-188 -RRB- denotes )
T20565 189-197 NN denotes reporter
T20566 198-206 NN denotes plasmids
T20567 207-216 NN denotes pTAL-SEAP
T20568 217-219 CC denotes or
T20569 220-231 NN denotes pNF-κB-SEAP
T20570 232-236 VB denotes were
T20571 237-248 VB denotes transfected
T20572 249-253 IN denotes into
T20573 254-259 NN denotes cells
T20574 260-265 VB denotes using
T20575 266-273 NN denotes Qiagene
T20576 274-283 NN denotes Effectene
T20577 284-286 CC denotes or
T20578 287-297 NN denotes Attractene
T20579 298-310 NN denotes transfection
T20580 311-314 NN denotes kit
T20581 315-316 -LRB- denotes (
T20582 316-322 NN denotes Qiagen
T20583 322-323 -COMMA- denotes ,
T20584 324-332 NNP denotes Valencia
T20585 332-333 -COMMA- denotes ,
T20586 334-336 NN denotes CA
T20587 336-337 -RRB- denotes )
T20588 338-347 VB denotes according
T20589 348-350 TO denotes to
T20590 351-354 DT denotes the
T20591 355-367 NN denotes manufacturer
T20592 367-369 POS denotes 's
T20593 370-378 NN denotes protocol
T20594 380-383 IN denotes For
T20595 384-399 NN denotes co-transfection
T20596 400-407 NN denotes studies
T20597 407-408 -COMMA- denotes ,
T20598 409-413 NN denotes PKCα
T20599 414-416 CC denotes or
T20600 417-422 NN denotes NF-κB
T20601 423-426 NN denotes p65
T20602 427-437 NN denotes constructs
T20603 438-442 VB denotes were
T20604 443-448 VB denotes mixed
T20605 449-451 IN denotes at
T20606 452-453 DT denotes a
T20607 454-459 NN denotes ratio
T20608 460-462 IN denotes of
T20609 463-466 NN denotes 5∶1
T20610 467-471 IN denotes with
T20611 472-475 DT denotes the
T20612 476-484 NN denotes reporter
T20613 485-492 NN denotes plasmid
T20614 494-497 IN denotes For
T20615 498-503 NN denotes siRNA
T20616 504-516 NN denotes transfection
T20617 516-517 -COMMA- denotes ,
T20618 518-521 CD denotes 100
T20619 522-524 NN denotes nM
T20620 525-527 IN denotes of
T20621 528-532 NN denotes PKCα
T20622 533-538 NN denotes siRNA
T20623 539-541 CC denotes or
T20624 542-549 NN denotes control
T20625 550-555 NN denotes siRNA
T20626 556-560 VB denotes were
T20627 561-565 VB denotes used
T20628 567-572 NN denotes Cells
T20629 573-577 VB denotes were
T20630 578-587 VB denotes incubated
T20631 588-592 IN denotes with
T20632 593-596 DT denotes the
T20633 597-609 NN denotes transfection
T20634 610-618 NN denotes reagents
T20635 619-622 IN denotes for
T20636 623-628 CD denotes 16–24
T20637 629-634 NN denotes hours
T20638 634-635 -COMMA- denotes ,
T20639 636-642 VB denotes washed
T20640 643-646 CC denotes and
T20641 647-654 VB denotes starved
T20642 655-657 IN denotes in
T20643 658-662 NN denotes RPMI
T20644 663-670 IN denotes without
T20645 671-674 NNP denotes FBS
T20646 675-678 IN denotes for
T20647 679-680 CD denotes 4
T20648 681-686 NN denotes hours
T20649 688-695 NN denotes Samples
T20650 696-700 VB denotes were
T20651 701-705 RB denotes then
T20652 706-716 VB denotes stimulated
T20653 717-721 IN denotes with
T20654 722-725 CD denotes 100
T20655 726-731 NN denotes ng/ml
T20656 732-737 NN denotes M-CSF
T20657 738-741 IN denotes for
T20658 742-743 CD denotes 4
T20659 744-749 NN denotes hours
T20660 750-752 IN denotes at
T20661 753-758 WDT denotes which
T20662 759-764 NN denotes point
T20663 765-768 DT denotes the
T20664 769-775 NN denotes medium
T20665 776-779 VB denotes was
T20666 780-789 VB denotes collected
T20667 790-793 IN denotes for
T20668 794-797 DT denotes the
T20669 798-802 NN denotes SEAP
T20670 803-811 NN denotes analysis
T20671 813-816 IN denotes For
T20672 817-827 NN denotes inhibition
T20673 828-835 NN denotes studies
T20674 835-836 -COMMA- denotes ,
T20675 837-842 NN denotes cells
T20676 843-847 VB denotes were
T20677 848-861 JJ denotes pre-incubated
T20678 862-866 IN denotes with
T20679 867-877 NN denotes inhibitors
T20680 878-881 IN denotes for
T20681 882-884 CD denotes 30
T20682 885-892 NN denotes minutes
T20683 893-899 IN denotes before
T20684 900-905 NN denotes M-CSF
T20685 906-915 NN denotes treatment
T20686 917-919 IN denotes On
T20687 920-927 NN denotes average
T20688 927-928 -COMMA- denotes ,
T20689 929-931 PRP denotes we
T20690 932-940 VB denotes realized
T20691 941-946 CD denotes 30–40
T20692 946-947 NN denotes %
T20693 948-960 NN denotes transfection
T20694 961-971 NN denotes efficiency
T20695 972-974 IN denotes as
T20696 975-984 VB denotes confirmed
T20697 985-987 IN denotes by
T20698 988-991 NN denotes GFP
T20699 992-1002 NN denotes expression
T20700 1003-1005 IN denotes in
T20701 1006-1009 NN denotes Raw
T20702 1010-1015 CD denotes 264.7
T20703 1016-1021 NN denotes cells
R15461 T20533 T20532 arg1Of Transfection,Transient
R15462 T20533 T20534 arg1Of Transfection,of
R15463 T20537 T20534 arg2Of Cells,of
R15464 T20537 T20535 arg1Of Cells,RAW
R15465 T20537 T20536 arg1Of Cells,264.7
R15466 T20540 T20538 arg1Of cells,RAW
R15467 T20540 T20539 arg1Of cells,264.7
R15468 T20540 T20541 arg1Of cells,were
R15469 T20540 T20542 arg2Of cells,seeded
R15470 T20542 T20541 arg2Of seeded,were
R15471 T20542 T20543 arg1Of seeded,in
R15472 T20542 T20554 arg1Of seeded,day
R15473 T20545 T20543 arg2Of plates,in
R15474 T20545 T20544 arg1Of plates,12-well
R15475 T20545 T20546 arg1Of plates,in
R15476 T20548 T20546 arg2Of medium,in
R15477 T20548 T20547 arg1Of medium,RPMI
R15478 T20548 T20549 arg1Of medium,containing
R15479 T20551 T20549 arg2Of %,containing
R15480 T20551 T20550 arg1Of %,5
R15481 T20552 T20553 arg1Of FBS,one
R15482 T20554 T20552 arg1Of day,FBS
R15483 T20554 T20555 arg1Of day,prior
R15484 T20554 T20570 modOf day,were
R15485 T20555 T20556 arg1Of prior,to
R15486 T20557 T20556 arg2Of transfection,to
R15487 T20557 T20558 arg1Of transfection,","
R15488 T20561 T20558 arg2Of phosphatase,","
R15489 T20561 T20559 arg2Of phosphatase,Secreted
R15490 T20561 T20560 arg1Of phosphatase,alkaline
R15491 T20561 T20562 arg1Of phosphatase,(
R15492 T20563 T20562 arg2Of SEAP,(
R15493 T20564 T20562 arg3Of ),(
R15494 T20567 T20568 arg1Of pTAL-SEAP,or
R15495 T20568 T20565 arg1Of or,reporter
R15496 T20568 T20566 arg1Of or,plasmids
R15497 T20568 T20570 arg1Of or,were
R15498 T20568 T20571 arg2Of or,transfected
R15499 T20569 T20568 arg2Of pNF-κB-SEAP,or
R15500 T20571 T20570 arg2Of transfected,were
R15501 T20571 T20572 arg1Of transfected,into
R15502 T20573 T20572 arg2Of cells,into
R15503 T20573 T20574 arg1Of cells,using
R15504 T20574 T20588 arg1Of using,according
R15505 T20576 T20575 arg1Of Effectene,Qiagene
R15506 T20576 T20577 arg1Of Effectene,or
R15507 T20577 T20574 arg2Of or,using
R15508 T20577 T20581 arg1Of or,(
R15509 T20580 T20577 arg2Of kit,or
R15510 T20580 T20578 arg1Of kit,Attractene
R15511 T20580 T20579 arg1Of kit,transfection
R15512 T20582 T20581 arg2Of Qiagen,(
R15513 T20582 T20583 arg1Of Qiagen,","
R15514 T20582 T20585 arg1Of Qiagen,","
R15515 T20584 T20583 arg2Of Valencia,","
R15516 T20586 T20585 arg2Of CA,","
R15517 T20587 T20581 arg3Of ),(
R15518 T20589 T20588 arg2Of to,according
R15519 T20591 T20590 arg1Of manufacturer,the
R15520 T20591 T20592 arg2Of manufacturer,'s
R15521 T20593 T20589 arg2Of protocol,to
R15522 T20593 T20592 arg1Of protocol,'s
R15523 T20596 T20594 arg2Of studies,For
R15524 T20596 T20595 arg1Of studies,co-transfection
R15525 T20598 T20599 arg1Of PKCα,or
R15526 T20600 T20599 arg2Of NF-κB,or
R15527 T20602 T20598 arg1Of constructs,PKCα
R15528 T20602 T20600 arg1Of constructs,NF-κB
R15529 T20602 T20601 arg1Of constructs,p65
R15530 T20602 T20603 arg1Of constructs,were
R15531 T20602 T20604 arg2Of constructs,mixed
R15532 T20604 T20594 arg1Of mixed,For
R15533 T20604 T20597 arg1Of mixed,","
R15534 T20604 T20603 arg2Of mixed,were
R15535 T20604 T20605 arg1Of mixed,at
R15536 T20607 T20605 arg2Of ratio,at
R15537 T20607 T20606 arg1Of ratio,a
R15538 T20607 T20608 arg1Of ratio,of
R15539 T20607 T20610 arg1Of ratio,with
R15540 T20609 T20608 arg2Of 5∶1,of
R15541 T20613 T20610 arg2Of plasmid,with
R15542 T20613 T20611 arg1Of plasmid,the
R15543 T20613 T20612 arg1Of plasmid,reporter
R15544 T20616 T20614 arg2Of transfection,For
R15545 T20616 T20615 arg1Of transfection,siRNA
R15546 T20619 T20618 arg1Of nM,100
R15547 T20619 T20620 arg1Of nM,of
R15548 T20619 T20626 arg1Of nM,were
R15549 T20619 T20627 arg2Of nM,used
R15550 T20622 T20621 arg1Of siRNA,PKCα
R15551 T20622 T20623 arg1Of siRNA,or
R15552 T20623 T20620 arg2Of or,of
R15553 T20625 T20623 arg2Of siRNA,or
R15554 T20625 T20624 arg1Of siRNA,control
R15555 T20627 T20614 arg1Of used,For
R15556 T20627 T20617 arg1Of used,","
R15557 T20627 T20626 arg2Of used,were
R15558 T20628 T20629 arg1Of Cells,were
R15559 T20628 T20630 arg2Of Cells,incubated
R15560 T20628 T20641 arg2Of Cells,starved
R15561 T20630 T20631 arg1Of incubated,with
R15562 T20630 T20640 arg1Of incubated,and
R15563 T20634 T20631 arg2Of reagents,with
R15564 T20634 T20632 arg1Of reagents,the
R15565 T20634 T20633 arg1Of reagents,transfection
R15566 T20634 T20635 arg1Of reagents,for
R15567 T20634 T20638 arg1Of reagents,","
R15568 T20634 T20639 arg2Of reagents,washed
R15569 T20637 T20635 arg2Of hours,for
R15570 T20637 T20636 arg1Of hours,16–24
R15571 T20640 T20629 arg2Of and,were
R15572 T20641 T20640 arg2Of starved,and
R15573 T20641 T20642 arg1Of starved,in
R15574 T20641 T20644 arg1Of starved,without
R15575 T20641 T20646 arg1Of starved,for
R15576 T20643 T20642 arg2Of RPMI,in
R15577 T20645 T20644 arg2Of FBS,without
R15578 T20648 T20646 arg2Of hours,for
R15579 T20648 T20647 arg1Of hours,4
R15580 T20649 T20650 arg1Of Samples,were
R15581 T20649 T20652 arg2Of Samples,stimulated
R15582 T20652 T20650 arg2Of stimulated,were
R15583 T20652 T20651 arg1Of stimulated,then
R15584 T20652 T20653 arg1Of stimulated,with
R15585 T20654 T20655 arg1Of 100,ng/ml
R15586 T20656 T20653 arg2Of M-CSF,with
R15587 T20656 T20654 arg1Of M-CSF,100
R15588 T20656 T20657 arg1Of M-CSF,for
R15589 T20656 T20661 arg2Of M-CSF,which
R15590 T20659 T20657 arg2Of hours,for
R15591 T20659 T20658 arg1Of hours,4
R15592 T20662 T20660 arg2Of point,at
R15593 T20662 T20661 arg1Of point,which
R15594 T20664 T20663 arg1Of medium,the
R15595 T20664 T20665 arg1Of medium,was
R15596 T20664 T20666 arg2Of medium,collected
R15597 T20666 T20660 arg1Of collected,at
R15598 T20666 T20665 arg2Of collected,was
R15599 T20666 T20667 arg1Of collected,for
R15600 T20670 T20667 arg2Of analysis,for
R15601 T20670 T20668 arg1Of analysis,the
R15602 T20670 T20669 arg1Of analysis,SEAP
R15603 T20673 T20671 arg2Of studies,For
R15604 T20673 T20672 arg1Of studies,inhibition
R15605 T20675 T20676 arg1Of cells,were
R15606 T20675 T20677 arg1Of cells,pre-incubated
R15607 T20676 T20671 arg1Of were,For
R15608 T20676 T20674 arg1Of were,","
R15609 T20677 T20676 arg2Of pre-incubated,were
R15610 T20677 T20678 arg1Of pre-incubated,with
R15611 T20679 T20678 arg2Of inhibitors,with
R15612 T20679 T20680 arg1Of inhibitors,for
R15613 T20682 T20680 arg2Of minutes,for
R15614 T20682 T20681 arg1Of minutes,30
R15615 T20682 T20683 arg1Of minutes,before
R15616 T20685 T20683 arg2Of treatment,before
R15617 T20685 T20684 arg1Of treatment,M-CSF
R15618 T20687 T20686 arg2Of average,On
R15619 T20689 T20690 arg1Of we,realized
R15620 T20690 T20686 arg1Of realized,On
R15621 T20690 T20688 arg1Of realized,","
R15622 T20690 T20695 arg1Of realized,as
R15623 T20694 T20690 arg2Of efficiency,realized
R15624 T20694 T20691 arg1Of efficiency,30–40
R15625 T20694 T20692 arg1Of efficiency,%
R15626 T20694 T20693 arg1Of efficiency,transfection
R15627 T20696 T20695 arg2Of confirmed,as
R15628 T20699 T20696 arg1Of expression,confirmed
R15629 T20699 T20697 arg2Of expression,by
R15630 T20699 T20698 arg1Of expression,GFP
R15631 T20699 T20700 arg1Of expression,in
R15632 T20703 T20700 arg2Of cells,in
R15633 T20703 T20701 arg1Of cells,Raw
R15634 T20703 T20702 arg1Of cells,264.7

sentences

Id Subject Object Predicate Lexical cue
T20341 0-41 Sentence denotes Transient Transfection of RAW 264.7 Cells
T20342 42-379 Sentence denotes RAW 264.7 cells were seeded in 12-well plates in RPMI medium containing 5% FBS one day prior to transfection, Secreted alkaline phosphatase (SEAP) reporter plasmids pTAL-SEAP or pNF-κB-SEAP were transfected into cells using Qiagene Effectene or Attractene transfection kit (Qiagen, Valencia, CA) according to the manufacturer's protocol.
T20343 380-493 Sentence denotes For co-transfection studies, PKCα or NF-κB p65 constructs were mixed at a ratio of 5∶1 with the reporter plasmid.
T20344 494-566 Sentence denotes For siRNA transfection, 100 nM of PKCα siRNA or control siRNA were used.
T20345 567-687 Sentence denotes Cells were incubated with the transfection reagents for 16–24 hours, washed and starved in RPMI without FBS for 4 hours.
T20346 688-812 Sentence denotes Samples were then stimulated with 100 ng/ml M-CSF for 4 hours at which point the medium was collected for the SEAP analysis.
T20347 813-916 Sentence denotes For inhibition studies, cells were pre-incubated with inhibitors for 30 minutes before M-CSF treatment.
T20348 917-1022 Sentence denotes On average, we realized 30–40% transfection efficiency as confirmed by GFP expression in Raw 264.7 cells.
T302 0-41 Sentence denotes Transient Transfection of RAW 264.7 Cells
T303 42-379 Sentence denotes RAW 264.7 cells were seeded in 12-well plates in RPMI medium containing 5% FBS one day prior to transfection, Secreted alkaline phosphatase (SEAP) reporter plasmids pTAL-SEAP or pNF-κB-SEAP were transfected into cells using Qiagene Effectene or Attractene transfection kit (Qiagen, Valencia, CA) according to the manufacturer's protocol.
T304 380-493 Sentence denotes For co-transfection studies, PKCα or NF-κB p65 constructs were mixed at a ratio of 5∶1 with the reporter plasmid.
T305 494-566 Sentence denotes For siRNA transfection, 100 nM of PKCα siRNA or control siRNA were used.
T306 567-687 Sentence denotes Cells were incubated with the transfection reagents for 16–24 hours, washed and starved in RPMI without FBS for 4 hours.
T307 688-812 Sentence denotes Samples were then stimulated with 100 ng/ml M-CSF for 4 hours at which point the medium was collected for the SEAP analysis.
T308 813-916 Sentence denotes For inhibition studies, cells were pre-incubated with inhibitors for 30 minutes before M-CSF treatment.
T309 917-1022 Sentence denotes On average, we realized 30–40% transfection efficiency as confirmed by GFP expression in Raw 264.7 cells.

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T20416 409-413 NNP denotes PKCα
T20349 0-9 NNP denotes Transient
T20350 10-22 NNP denotes Transfection
T20351 23-25 IN denotes of
T20352 26-29 NNP denotes RAW
T20353 30-35 CD denotes 264.7
T20354 36-41 NNP denotes Cells
T20355 42-45 NNP denotes RAW
T20356 46-51 CD denotes 264.7
T20357 52-57 NNS denotes cells
T20358 58-62 VBD denotes were
T20359 63-69 VBN denotes seeded
T20360 70-72 IN denotes in
T20361 73-80 JJ denotes 12-well
T20362 81-87 NNS denotes plates
T20363 88-90 IN denotes in
T20364 91-95 NNP denotes RPMI
T20365 96-102 NN denotes medium
T20366 103-113 VBG denotes containing
T20367 114-115 CD denotes 5
T20368 115-116 NN denotes %
T20369 117-120 NNP denotes FBS
T20370 121-124 CD denotes one
T20371 125-128 NN denotes day
T20372 129-134 RB denotes prior
T20373 135-137 TO denotes to
T20374 138-150 NN denotes transfection
T20375 150-151 , denotes ,
T20376 152-160 NNP denotes Secreted
T20377 161-169 NN denotes alkaline
T20378 170-181 NN denotes phosphatase
T20379 182-183 -LRB- denotes (
T20380 183-187 NNP denotes SEAP
T20381 187-188 -RRB- denotes )
T20382 189-197 NN denotes reporter
T20383 198-206 NNS denotes plasmids
T20384 207-216 JJ denotes pTAL-SEAP
T20385 217-219 CC denotes or
T20386 220-231 JJ denotes pNF-κB-SEAP
T20387 232-236 VBD denotes were
T20388 237-248 VBN denotes transfected
T20389 249-253 IN denotes into
T20390 254-259 NNS denotes cells
T20391 260-265 VBG denotes using
T20392 266-273 NNP denotes Qiagene
T20393 274-283 NNP denotes Effectene
T20394 284-286 CC denotes or
T20395 287-297 NNP denotes Attractene
T20396 298-310 NN denotes transfection
T20397 311-314 NN denotes kit
T20398 315-316 -LRB- denotes (
T20399 316-322 NNP denotes Qiagen
T20400 322-323 , denotes ,
T20401 324-332 NNP denotes Valencia
T20402 332-333 , denotes ,
T20403 334-336 NNP denotes CA
T20404 336-337 -RRB- denotes )
T20405 338-347 VBG denotes according
T20406 348-350 TO denotes to
T20407 351-354 DT denotes the
T20408 355-367 NN denotes manufacturer
T20409 367-369 POS denotes 's
T20410 370-378 NN denotes protocol
T20411 378-379 . denotes .
T20412 380-383 IN denotes For
T20413 384-399 JJ denotes co-transfection
T20414 400-407 NNS denotes studies
T20415 407-408 , denotes ,
T20417 414-416 CC denotes or
T20418 417-422 NNP denotes NF-κB
T20419 423-426 CD denotes p65
T20420 427-437 NNS denotes constructs
T20421 438-442 VBD denotes were
T20422 443-448 VBN denotes mixed
T20423 449-451 IN denotes at
T20424 452-453 DT denotes a
T20425 454-459 NN denotes ratio
T20426 460-462 IN denotes of
T20427 463-464 CD denotes 5
T20428 464-465 CD denotes ∶
T20429 465-466 CD denotes 1
T20430 467-471 IN denotes with
T20431 472-475 DT denotes the
T20432 476-484 NN denotes reporter
T20433 485-492 NN denotes plasmid
T20434 492-493 . denotes .
T20435 494-497 IN denotes For
T20436 498-503 JJ denotes siRNA
T20437 504-516 NN denotes transfection
T20438 516-517 , denotes ,
T20439 518-521 CD denotes 100
T20440 522-524 NN denotes nM
T20441 525-527 IN denotes of
T20442 528-532 NNP denotes PKCα
T20443 533-538 NNP denotes siRNA
T20444 539-541 CC denotes or
T20445 542-549 VB denotes control
T20446 550-555 NNP denotes siRNA
T20447 556-560 VBD denotes were
T20448 561-565 VBN denotes used
T20449 565-566 . denotes .
T20450 567-572 NNS denotes Cells
T20451 573-577 VBD denotes were
T20452 578-587 VBN denotes incubated
T20453 588-592 IN denotes with
T20454 593-596 DT denotes the
T20455 597-609 NN denotes transfection
T20456 610-618 VBZ denotes reagents
T20457 619-622 IN denotes for
T20458 623-625 CD denotes 16
T20459 626-628 CD denotes 24
T20460 629-634 NNS denotes hours
T20461 634-635 , denotes ,
T20462 636-642 VBD denotes washed
T20463 643-646 CC denotes and
T20464 647-654 VBD denotes starved
T20465 655-657 IN denotes in
T20466 658-662 NNP denotes RPMI
T20467 663-670 IN denotes without
T20468 671-674 NNP denotes FBS
T20469 675-678 IN denotes for
T20470 679-680 CD denotes 4
T20471 681-686 NNS denotes hours
T20472 686-687 . denotes .
T20473 688-695 NNS denotes Samples
T20474 696-700 VBD denotes were
T20475 701-705 RB denotes then
T20476 706-716 VBN denotes stimulated
T20477 717-721 IN denotes with
T20478 722-725 CD denotes 100
T20479 726-731 JJ denotes ng/ml
T20480 732-737 NN denotes M-CSF
T20481 738-741 IN denotes for
T20482 742-743 CD denotes 4
T20483 744-749 NNS denotes hours
T20484 750-752 IN denotes at
T20485 753-758 WDT denotes which
T20486 759-764 VBP denotes point
T20487 765-768 DT denotes the
T20488 769-775 NN denotes medium
T20489 776-779 VBD denotes was
T20490 780-789 VBN denotes collected
T20491 790-793 IN denotes for
T20492 794-797 DT denotes the
T20493 798-802 NNP denotes SEAP
T20494 803-811 NN denotes analysis
T20495 811-812 . denotes .
T20496 813-816 IN denotes For
T20497 817-827 NN denotes inhibition
T20498 828-835 NNS denotes studies
T20499 835-836 , denotes ,
T20500 837-842 NNS denotes cells
T20501 843-847 VBD denotes were
T20502 848-861 VBN denotes pre-incubated
T20503 862-866 IN denotes with
T20504 867-877 NNS denotes inhibitors
T20505 878-881 IN denotes for
T20506 882-884 CD denotes 30
T20507 885-892 NNS denotes minutes
T20508 893-899 IN denotes before
T20509 900-905 NNP denotes M-CSF
T20510 906-915 NN denotes treatment
T20511 915-916 . denotes .
T20512 917-919 IN denotes On
T20513 920-927 NN denotes average
T20514 927-928 , denotes ,
T20515 929-931 PRP denotes we
T20516 932-940 VBD denotes realized
T20517 941-943 CD denotes 30
T20518 944-946 CD denotes 40
T20519 946-947 NN denotes %
T20520 948-960 NN denotes transfection
T20521 961-971 NN denotes efficiency
T20522 972-974 IN denotes as
T20523 975-984 VBN denotes confirmed
T20524 985-987 IN denotes by
T20525 988-991 NNP denotes GFP
T20526 992-1002 NN denotes expression
T20527 1003-1005 IN denotes in
T20528 1006-1009 NNP denotes Raw
T20529 1010-1015 CD denotes 264.7
T20530 1016-1021 NNS denotes cells
T20531 1021-1022 . denotes .
R15278 T20349 T20350 compound Transient,Transfection
R15279 T20350 T20359 nsubjpass Transfection,seeded
R15280 T20351 T20350 prep of,Transfection
R15281 T20352 T20351 pobj RAW,of
R15282 T20353 T20355 nummod 264.7,RAW
R15283 T20354 T20355 compound Cells,RAW
R15284 T20355 T20350 appos RAW,Transfection
R15285 T20356 T20357 nummod 264.7,cells
R15286 T20357 T20359 nsubjpass cells,seeded
R15287 T20358 T20359 auxpass were,seeded
R15288 T20359 T20359 ROOT seeded,seeded
R15289 T20360 T20359 prep in,seeded
R15290 T20361 T20362 amod 12-well,plates
R15291 T20362 T20360 pobj plates,in
R15292 T20363 T20359 prep in,seeded
R15293 T20364 T20365 compound RPMI,medium
R15294 T20365 T20363 pobj medium,in
R15295 T20366 T20365 acl containing,medium
R15296 T20367 T20368 nummod 5,%
R15297 T20368 T20366 dobj %,containing
R15298 T20369 T20372 nsubj FBS,prior
R15299 T20370 T20371 nummod one,day
R15300 T20371 T20372 npadvmod day,prior
R15301 T20372 T20368 amod prior,%
R15302 T20373 T20372 prep to,prior
R15303 T20374 T20373 pobj transfection,to
R15304 T20375 T20359 punct ",",seeded
R15305 T20376 T20378 compound Secreted,phosphatase
R15306 T20377 T20378 compound alkaline,phosphatase
R15307 T20378 T20388 nsubjpass phosphatase,transfected
R15308 T20379 T20380 punct (,SEAP
R15309 T20380 T20378 appos SEAP,phosphatase
R15310 T20381 T20380 punct ),SEAP
R15311 T20382 T20383 compound reporter,plasmids
R15312 T20383 T20384 compound plasmids,pTAL-SEAP
R15313 T20384 T20378 conj pTAL-SEAP,phosphatase
R15314 T20385 T20384 cc or,pTAL-SEAP
R15315 T20386 T20384 conj pNF-κB-SEAP,pTAL-SEAP
R15316 T20387 T20388 auxpass were,transfected
R15317 T20388 T20359 dep transfected,seeded
R15318 T20389 T20388 prep into,transfected
R15319 T20390 T20389 pobj cells,into
R15320 T20391 T20390 acl using,cells
R15321 T20392 T20393 compound Qiagene,Effectene
R15322 T20393 T20391 dobj Effectene,using
R15323 T20394 T20393 cc or,Effectene
R15324 T20395 T20397 compound Attractene,kit
R15325 T20396 T20397 compound transfection,kit
R15326 T20397 T20393 conj kit,Effectene
R15327 T20398 T20399 punct (,Qiagen
R15328 T20399 T20397 appos Qiagen,kit
R15329 T20400 T20399 punct ",",Qiagen
R15330 T20401 T20399 npadvmod Valencia,Qiagen
R15331 T20402 T20401 punct ",",Valencia
R15332 T20403 T20401 appos CA,Valencia
R15333 T20404 T20399 punct ),Qiagen
R15334 T20405 T20388 prep according,transfected
R15335 T20406 T20405 prep to,according
R15336 T20407 T20408 det the,manufacturer
R15337 T20408 T20410 poss manufacturer,protocol
R15338 T20409 T20408 case 's,manufacturer
R15339 T20410 T20406 pobj protocol,to
R15340 T20411 T20388 punct .,transfected
R15341 T20412 T20421 prep For,were
R15342 T20413 T20414 amod co-transfection,studies
R15343 T20414 T20412 pobj studies,For
R15344 T20415 T20421 punct ",",were
R15345 T20416 T20420 nmod PKCα,constructs
R15346 T20417 T20416 cc or,PKCα
R15347 T20418 T20416 conj NF-κB,PKCα
R15348 T20419 T20418 nummod p65,NF-κB
R15349 T20420 T20421 nsubj constructs,were
R15350 T20421 T20421 ROOT were,were
R15351 T20422 T20421 acomp mixed,were
R15352 T20423 T20422 prep at,mixed
R15353 T20424 T20425 det a,ratio
R15354 T20425 T20423 pobj ratio,at
R15355 T20426 T20425 prep of,ratio
R15356 T20427 T20429 nummod 5,1
R15357 T20428 T20429 nummod ∶,1
R15358 T20429 T20426 pobj 1,of
R15359 T20430 T20425 prep with,ratio
R15360 T20431 T20433 det the,plasmid
R15361 T20432 T20433 compound reporter,plasmid
R15362 T20433 T20430 pobj plasmid,with
R15363 T20434 T20421 punct .,were
R15364 T20435 T20448 prep For,used
R15365 T20436 T20437 amod siRNA,transfection
R15366 T20437 T20435 pobj transfection,For
R15367 T20438 T20448 punct ",",used
R15368 T20439 T20440 nummod 100,nM
R15369 T20440 T20448 nsubjpass nM,used
R15370 T20441 T20440 prep of,nM
R15371 T20442 T20443 compound PKCα,siRNA
R15372 T20443 T20441 pobj siRNA,of
R15373 T20444 T20440 cc or,nM
R15374 T20445 T20446 compound control,siRNA
R15375 T20446 T20440 conj siRNA,nM
R15376 T20447 T20448 auxpass were,used
R15377 T20448 T20448 ROOT used,used
R15378 T20449 T20448 punct .,used
R15379 T20450 T20452 nsubjpass Cells,incubated
R15380 T20451 T20452 auxpass were,incubated
R15381 T20452 T20452 ROOT incubated,incubated
R15382 T20453 T20452 prep with,incubated
R15383 T20454 T20456 det the,reagents
R15384 T20455 T20456 compound transfection,reagents
R15385 T20456 T20453 pobj reagents,with
R15386 T20457 T20452 prep for,incubated
R15387 T20458 T20457 pobj 16,for
R15388 T20459 T20460 nummod 24,hours
R15389 T20460 T20458 appos hours,16
R15390 T20461 T20452 punct ",",incubated
R15391 T20462 T20452 conj washed,incubated
R15392 T20463 T20462 cc and,washed
R15393 T20464 T20462 conj starved,washed
R15394 T20465 T20464 prep in,starved
R15395 T20466 T20465 pobj RPMI,in
R15396 T20467 T20464 prep without,starved
R15397 T20468 T20467 pobj FBS,without
R15398 T20469 T20464 prep for,starved
R15399 T20470 T20471 nummod 4,hours
R15400 T20471 T20469 pobj hours,for
R15401 T20472 T20452 punct .,incubated
R15402 T20473 T20476 nsubjpass Samples,stimulated
R15403 T20474 T20476 auxpass were,stimulated
R15404 T20475 T20476 advmod then,stimulated
R15405 T20476 T20476 ROOT stimulated,stimulated
R15406 T20477 T20476 prep with,stimulated
R15407 T20478 T20480 nummod 100,M-CSF
R15408 T20479 T20480 amod ng/ml,M-CSF
R15409 T20480 T20477 pobj M-CSF,with
R15410 T20481 T20480 prep for,M-CSF
R15411 T20482 T20483 nummod 4,hours
R15412 T20483 T20481 pobj hours,for
R15413 T20484 T20486 preconj at,point
R15414 T20485 T20486 det which,point
R15415 T20486 T20483 relcl point,hours
R15416 T20487 T20488 det the,medium
R15417 T20488 T20490 nsubjpass medium,collected
R15418 T20489 T20490 auxpass was,collected
R15419 T20490 T20476 conj collected,stimulated
R15420 T20491 T20490 prep for,collected
R15421 T20492 T20494 det the,analysis
R15422 T20493 T20494 compound SEAP,analysis
R15423 T20494 T20491 pobj analysis,for
R15424 T20495 T20490 punct .,collected
R15425 T20496 T20502 prep For,pre-incubated
R15426 T20497 T20498 compound inhibition,studies
R15427 T20498 T20496 pobj studies,For
R15428 T20499 T20502 punct ",",pre-incubated
R15429 T20500 T20502 nsubjpass cells,pre-incubated
R15430 T20501 T20502 auxpass were,pre-incubated
R15431 T20502 T20502 ROOT pre-incubated,pre-incubated
R15432 T20503 T20502 prep with,pre-incubated
R15433 T20504 T20503 pobj inhibitors,with
R15434 T20505 T20504 prep for,inhibitors
R15435 T20506 T20507 nummod 30,minutes
R15436 T20507 T20505 pobj minutes,for
R15437 T20508 T20507 prep before,minutes
R15438 T20509 T20510 compound M-CSF,treatment
R15439 T20510 T20508 pobj treatment,before
R15440 T20511 T20502 punct .,pre-incubated
R15441 T20512 T20516 prep On,realized
R15442 T20513 T20512 amod average,On
R15443 T20514 T20516 punct ",",realized
R15444 T20515 T20516 nsubj we,realized
R15445 T20516 T20516 ROOT realized,realized
R15446 T20517 T20516 dobj 30,realized
R15447 T20518 T20519 nummod 40,%
R15448 T20519 T20521 nmod %,efficiency
R15449 T20520 T20521 compound transfection,efficiency
R15450 T20521 T20516 conj efficiency,realized
R15451 T20522 T20523 mark as,confirmed
R15452 T20523 T20521 advcl confirmed,efficiency
R15453 T20524 T20523 agent by,confirmed
R15454 T20525 T20526 compound GFP,expression
R15455 T20526 T20524 pobj expression,by
R15456 T20527 T20523 prep in,confirmed
R15457 T20528 T20530 amod Raw,cells
R15458 T20529 T20530 nummod 264.7,cells
R15459 T20530 T20527 pobj cells,in
R15460 T20531 T20516 punct .,realized

test2

Id Subject Object Predicate Lexical cue
T20334 409-413 Protein denotes PKCα
T20335 423-426 Protein denotes p65
T20336 528-538 Protein denotes PKCα siRNA
T20337 732-737 Protein denotes M-CSF
T20338 900-905 Protein denotes M-CSF
T20339 988-991 Protein denotes GFP
T20340 992-1002 Gene_expression denotes expression
R15277 T20339 T20340 themeOf GFP,expression