PMC:3245220 / 42040-42664 JSONTXT

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T19886","span":{"begin":317,"end":321},"obj":"P08571"},{"id":"T19887","span":{"begin":618,"end":623},"obj":"P09603"},{"id":"T19888","span":{"begin":620,"end":623},"obj":"P04141"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Purification of Peripheral Blood Monocytes and Monocyte-Derived Macrophages (MDMs)\nMonocytes were isolated from source leukocyte packs obtained from the American Red Cross as described previously [51]. Monocytes used in real time PCR experiments and transfection experiments were purified by positive selection using CD14 Monocyte Isolation Kit from Miltenyi Biotech (Auburn, CA) (\u003e90% pure). In some experiments, monocytes were isolated by clumping method (70% pure). To obtain monocyte-derived macrophages (MDMs), monocytes were cultured in RPMI-1640 medium containing 10% FBS, and 10 µg/ml polymyxin B and 20 ng/ml M-CSF."}

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{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T19910","span":{"begin":317,"end":344},"obj":"Protein"}],"text":"Purification of Peripheral Blood Monocytes and Monocyte-Derived Macrophages (MDMs)\nMonocytes were isolated from source leukocyte packs obtained from the American Red Cross as described previously [51]. Monocytes used in real time PCR experiments and transfection experiments were purified by positive selection using CD14 Monocyte Isolation Kit from Miltenyi Biotech (Auburn, CA) (\u003e90% pure). In some experiments, monocytes were isolated by clumping method (70% pure). To obtain monocyte-derived macrophages (MDMs), monocytes were cultured in RPMI-1640 medium containing 10% FBS, and 10 µg/ml polymyxin B and 20 ng/ml M-CSF."}

{"project":"events-check-again","denotations":[{"id":"T19908","span":{"begin":317,"end":321},"obj":"Protein"},{"id":"T19909","span":{"begin":618,"end":623},"obj":"Protein"}],"text":"Purification of Peripheral Blood Monocytes and Monocyte-Derived Macrophages (MDMs)\nMonocytes were isolated from source leukocyte packs obtained from the American Red Cross as described previously [51]. Monocytes used in real time PCR experiments and transfection experiments were purified by positive selection using CD14 Monocyte Isolation Kit from Miltenyi Biotech (Auburn, CA) (\u003e90% pure). In some experiments, monocytes were isolated by clumping method (70% pure). To obtain monocyte-derived macrophages (MDMs), monocytes were cultured in RPMI-1640 medium containing 10% FBS, and 10 µg/ml polymyxin B and 20 ng/ml M-CSF."}

{"project":"GO-MF","denotations":[{"id":"T19897","span":{"begin":376,"end":378},"obj":"http://purl.obolibrary.org/obo/GO_0033968"}],"text":"Purification of Peripheral Blood Monocytes and Monocyte-Derived Macrophages (MDMs)\nMonocytes were isolated from source leukocyte packs obtained from the American Red Cross as described previously [51]. Monocytes used in real time PCR experiments and transfection experiments were purified by positive selection using CD14 Monocyte Isolation Kit from Miltenyi Biotech (Auburn, CA) (\u003e90% pure). In some experiments, monocytes were isolated by clumping method (70% pure). To obtain monocyte-derived macrophages (MDMs), monocytes were cultured in RPMI-1640 medium containing 10% FBS, and 10 µg/ml polymyxin B and 20 ng/ml M-CSF."}

{"project":"GO-BP","denotations":[{"id":"T19896","span":{"begin":376,"end":378},"obj":"http://purl.obolibrary.org/obo/GO_0033968"}],"text":"Purification of Peripheral Blood Monocytes and Monocyte-Derived Macrophages (MDMs)\nMonocytes were isolated from source leukocyte packs obtained from the American Red Cross as described previously [51]. Monocytes used in real time PCR experiments and transfection experiments were purified by positive selection using CD14 Monocyte Isolation Kit from Miltenyi Biotech (Auburn, CA) (\u003e90% pure). In some experiments, monocytes were isolated by clumping method (70% pure). To obtain monocyte-derived macrophages (MDMs), monocytes were cultured in RPMI-1640 medium containing 10% FBS, and 10 µg/ml polymyxin B and 20 ng/ml M-CSF."}

{"project":"UBERON-AE","denotations":[{"id":"T19660","span":{"begin":27,"end":32},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"}],"text":"Purification of Peripheral Blood Monocytes and Monocyte-Derived Macrophages (MDMs)\nMonocytes were isolated from source leukocyte packs obtained from the American Red Cross as described previously [51]. Monocytes used in real time PCR experiments and transfection experiments were purified by positive selection using CD14 Monocyte Isolation Kit from Miltenyi Biotech (Auburn, CA) (\u003e90% pure). In some experiments, monocytes were isolated by clumping method (70% pure). To obtain monocyte-derived macrophages (MDMs), monocytes were cultured in RPMI-1640 medium containing 10% FBS, and 10 µg/ml polymyxin B and 20 ng/ml M-CSF."}

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of Peripheral Blood Monocytes and Monocyte-Derived Macrophages (MDMs)\nMonocytes were isolated from source leukocyte packs obtained from the American Red Cross as described previously [51]. Monocytes used in real time PCR experiments and transfection experiments were purified by positive selection using CD14 Monocyte Isolation Kit from Miltenyi Biotech (Auburn, CA) (\u003e90% pure). In some experiments, monocytes were isolated by clumping method (70% pure). To obtain monocyte-derived macrophages (MDMs), monocytes were cultured in RPMI-1640 medium containing 10% FBS, and 10 µg/ml polymyxin B and 20 ng/ml M-CSF."}

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