PMC:3245220 / 40128-41543 JSONTXT

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T19255","span":{"begin":28,"end":33},"obj":"P09603"},{"id":"T19256","span":{"begin":30,"end":33},"obj":"P04141"},{"id":"T19257","span":{"begin":503,"end":507},"obj":"P17252"},{"id":"T19258","span":{"begin":514,"end":517},"obj":"Q04206"},{"id":"T19259","span":{"begin":514,"end":517},"obj":"P21579"},{"id":"T19260","span":{"begin":528,"end":535},"obj":"P60709"},{"id":"T19261","span":{"begin":541,"end":546},"obj":"P04406"},{"id":"T19262","span":{"begin":616,"end":620},"obj":"P17252"},{"id":"T19263","span":{"begin":702,"end":705},"obj":"P21579"},{"id":"T19264","span":{"begin":702,"end":705},"obj":"Q04206"},{"id":"T19265","span":{"begin":921,"end":924},"obj":"Q04206"},{"id":"T19266","span":{"begin":921,"end":924},"obj":"P21579"},{"id":"T19267","span":{"begin":933,"end":936},"obj":"P21579"},{"id":"T19268","span":{"begin":933,"end":936},"obj":"Q04206"},{"id":"T19269","span":{"begin":952,"end":955},"obj":"Q04206"},{"id":"T19270","span":{"begin":952,"end":955},"obj":"P21579"},{"id":"T19271","span":{"begin":1069,"end":1073},"obj":"P17252"},{"id":"T19272","span":{"begin":1078,"end":1082},"obj":"P17252"},{"id":"T19273","span":{"begin":1102,"end":1106},"obj":"P17252"},{"id":"T19274","span":{"begin":1111,"end":1115},"obj":"P17252"},{"id":"T19275","span":{"begin":1205,"end":1209},"obj":"P25963"},{"id":"T19276","span":{"begin":1211,"end":1217},"obj":"P25963"},{"id":"T19277","span":{"begin":1223,"end":1228},"obj":"P04406"},{"id":"T19278","span":{"begin":1333,"end":1337},"obj":"P17252"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Materials\nRecombinant human M-CSF was purchased from R\u0026D Systems (Minneapolis, MN). Ro-31-8220, Gö-6976, Rotterin, and BAPTA/AM were obtained from Calbiochem (San Diego, CA). Low endotoxin RPMI and X-vivo serum free medium were obtained from Lonza Walkersville Inc (Walkersville, MD). Fetal bovine serum (FBS, certified \u003c0.06 endotoxin units/ml endotoxin levels) was purchased from Atlanta Biological (Lawrenceville, GA). All other cell culture reagents were obtained through Invitrogen (Carlsbad, CA). PKCα NF-κB p65, Lamin B, β-actin, and GAPDH antibodies, anti-mouse and rabbit IgG-HRP conjugated antibodies, and PKCα siRNA were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Phospho-NFκB p65 (Ser276 or Ser536) antibodies were purchased from Cell Signaling Technology (Danvers, MA). DNA constructs pTAL-SEAP and pNF-κB-SEAP were obtained from Clontech (Mountian View, CA). Expression vectors containing CMV-p65 WT, CMV-p65 276S/A and CMV-p65 536S/A were cloned in pBa5e Puro vector or pFLAG-CMV-2 vector as described previously [49]. Kinase deficient CMV-PKCα (KD-PKCα) or wild type pCMV-PKCα (WT-PKCα) were generous gifts from Alexandra C. Newton (University of California, San Diego, CA). IκBα (NFKBIA) and GAPDH primers for real-time RT-PCR were obtained from SABiosciences (Frederick, MD). A pool of mouse or human PKCα specific siRNA were purchased from Santa Cruz Biotechnology (Santa cruz, CA)."}

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T19279","span":{"begin":1048,"end":1064},"obj":"Negative_regulation"},{"id":"T19280","span":{"begin":1075,"end":1077},"obj":"Negative_regulation"},{"id":"T19281","span":{"begin":616,"end":626},"obj":"Protein"},{"id":"T19282","span":{"begin":1333,"end":1352},"obj":"Protein"},{"id":"T19283","span":{"begin":921,"end":924},"obj":"Protein"},{"id":"T19284","span":{"begin":933,"end":936},"obj":"Protein"},{"id":"T19285","span":{"begin":952,"end":955},"obj":"Protein"},{"id":"T19286","span":{"begin":1069,"end":1073},"obj":"Protein"},{"id":"T19287","span":{"begin":1078,"end":1082},"obj":"Protein"},{"id":"T19288","span":{"begin":1102,"end":1106},"obj":"Protein"},{"id":"T19289","span":{"begin":1111,"end":1115},"obj":"Protein"},{"id":"T19290","span":{"begin":1205,"end":1209},"obj":"Protein"},{"id":"T19291","span":{"begin":28,"end":33},"obj":"Protein"}],"relations":[{"id":"R14454","pred":"themeOf","subj":"T19286","obj":"T19279"},{"id":"R14455","pred":"themeOf","subj":"T19287","obj":"T19280"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Materials\nRecombinant human M-CSF was purchased from R\u0026D Systems (Minneapolis, MN). Ro-31-8220, Gö-6976, Rotterin, and BAPTA/AM were obtained from Calbiochem (San Diego, CA). Low endotoxin RPMI and X-vivo serum free medium were obtained from Lonza Walkersville Inc (Walkersville, MD). Fetal bovine serum (FBS, certified \u003c0.06 endotoxin units/ml endotoxin levels) was purchased from Atlanta Biological (Lawrenceville, GA). All other cell culture reagents were obtained through Invitrogen (Carlsbad, CA). PKCα NF-κB p65, Lamin B, β-actin, and GAPDH antibodies, anti-mouse and rabbit IgG-HRP conjugated antibodies, and PKCα siRNA were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Phospho-NFκB p65 (Ser276 or Ser536) antibodies were purchased from Cell Signaling Technology (Danvers, MA). DNA constructs pTAL-SEAP and pNF-κB-SEAP were obtained from Clontech (Mountian View, CA). Expression vectors containing CMV-p65 WT, CMV-p65 276S/A and CMV-p65 536S/A were cloned in pBa5e Puro vector or pFLAG-CMV-2 vector as described previously [49]. Kinase deficient CMV-PKCα (KD-PKCα) or wild type pCMV-PKCα (WT-PKCα) were generous gifts from Alexandra C. Newton (University of California, San Diego, CA). IκBα (NFKBIA) and GAPDH primers for real-time RT-PCR were obtained from SABiosciences (Frederick, MD). A pool of mouse or human PKCα specific siRNA were purchased from Santa Cruz Biotechnology (Santa cruz, CA)."}

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T19408","span":{"begin":10,"end":33},"obj":"Protein"},{"id":"T19409","span":{"begin":189,"end":193},"obj":"Protein"},{"id":"T19410","span":{"begin":503,"end":513},"obj":"Protein"},{"id":"T19411","span":{"begin":514,"end":517},"obj":"Protein"},{"id":"T19412","span":{"begin":519,"end":526},"obj":"Protein"},{"id":"T19413","span":{"begin":528,"end":535},"obj":"Protein"},{"id":"T19414","span":{"begin":541,"end":557},"obj":"Protein"},{"id":"T19415","span":{"begin":574,"end":610},"obj":"Protein"},{"id":"T19416","span":{"begin":616,"end":626},"obj":"Protein"},{"id":"T19417","span":{"begin":702,"end":705},"obj":"Protein"},{"id":"T19418","span":{"begin":826,"end":832},"obj":"Protein"},{"id":"T19419","span":{"begin":833,"end":837},"obj":"Protein"},{"id":"T19420","span":{"begin":1069,"end":1073},"obj":"Protein"},{"id":"T19421","span":{"begin":1075,"end":1082},"obj":"Protein"},{"id":"T19422","span":{"begin":1102,"end":1106},"obj":"Protein"},{"id":"T19423","span":{"begin":1108,"end":1115},"obj":"Protein"},{"id":"T19424","span":{"begin":1055,"end":1064},"obj":"Negative_regulation"},{"id":"T19425","span":{"begin":1055,"end":1064},"obj":"Negative_regulation"},{"id":"T19426","span":{"begin":1055,"end":1064},"obj":"Negative_regulation"},{"id":"T19427","span":{"begin":1205,"end":1209},"obj":"Protein"},{"id":"T19428","span":{"begin":1211,"end":1217},"obj":"Protein"},{"id":"T19429","span":{"begin":1223,"end":1228},"obj":"Protein"},{"id":"T19430","span":{"begin":1333,"end":1352},"obj":"Protein"}],"relations":[{"id":"R14473","pred":"themeOf","subj":"T19420","obj":"T19424"},{"id":"R14474","pred":"themeOf","subj":"T19421","obj":"T19425"},{"id":"R14475","pred":"themeOf","subj":"T19422","obj":"T19426"}],"text":"Materials\nRecombinant human M-CSF was purchased from R\u0026D Systems (Minneapolis, MN). Ro-31-8220, Gö-6976, Rotterin, and BAPTA/AM were obtained from Calbiochem (San Diego, CA). Low endotoxin RPMI and X-vivo serum free medium were obtained from Lonza Walkersville Inc (Walkersville, MD). Fetal bovine serum (FBS, certified \u003c0.06 endotoxin units/ml endotoxin levels) was purchased from Atlanta Biological (Lawrenceville, GA). All other cell culture reagents were obtained through Invitrogen (Carlsbad, CA). PKCα NF-κB p65, Lamin B, β-actin, and GAPDH antibodies, anti-mouse and rabbit IgG-HRP conjugated antibodies, and PKCα siRNA were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Phospho-NFκB p65 (Ser276 or Ser536) antibodies were purchased from Cell Signaling Technology (Danvers, MA). DNA constructs pTAL-SEAP and pNF-κB-SEAP were obtained from Clontech (Mountian View, CA). Expression vectors containing CMV-p65 WT, CMV-p65 276S/A and CMV-p65 536S/A were cloned in pBa5e Puro vector or pFLAG-CMV-2 vector as described previously [49]. Kinase deficient CMV-PKCα (KD-PKCα) or wild type pCMV-PKCα (WT-PKCα) were generous gifts from Alexandra C. Newton (University of California, San Diego, CA). IκBα (NFKBIA) and GAPDH primers for real-time RT-PCR were obtained from SABiosciences (Frederick, MD). A pool of mouse or human PKCα specific siRNA were purchased from Santa Cruz Biotechnology (Santa cruz, CA)."}

{"project":"events-check-again","denotations":[{"id":"T19395","span":{"begin":28,"end":33},"obj":"Protein"},{"id":"T19396","span":{"begin":616,"end":626},"obj":"Protein"},{"id":"T19397","span":{"begin":921,"end":924},"obj":"Protein"},{"id":"T19398","span":{"begin":933,"end":936},"obj":"Protein"},{"id":"T19399","span":{"begin":952,"end":955},"obj":"Protein"},{"id":"T19400","span":{"begin":1048,"end":1064},"obj":"Negative_regulation"},{"id":"T19401","span":{"begin":1069,"end":1073},"obj":"Protein"},{"id":"T19402","span":{"begin":1075,"end":1077},"obj":"Negative_regulation"},{"id":"T19403","span":{"begin":1078,"end":1082},"obj":"Protein"},{"id":"T19404","span":{"begin":1102,"end":1106},"obj":"Protein"},{"id":"T19405","span":{"begin":1111,"end":1115},"obj":"Protein"},{"id":"T19406","span":{"begin":1205,"end":1209},"obj":"Protein"},{"id":"T19407","span":{"begin":1333,"end":1352},"obj":"Protein"}],"relations":[{"id":"R14471","pred":"themeOf","subj":"T19401","obj":"T19400"},{"id":"R14472","pred":"themeOf","subj":"T19403","obj":"T19402"}],"text":"Materials\nRecombinant human M-CSF was purchased from R\u0026D Systems (Minneapolis, MN). Ro-31-8220, Gö-6976, Rotterin, and BAPTA/AM were obtained from Calbiochem (San Diego, CA). Low endotoxin RPMI and X-vivo serum free medium were obtained from Lonza Walkersville Inc (Walkersville, MD). Fetal bovine serum (FBS, certified \u003c0.06 endotoxin units/ml endotoxin levels) was purchased from Atlanta Biological (Lawrenceville, GA). All other cell culture reagents were obtained through Invitrogen (Carlsbad, CA). PKCα NF-κB p65, Lamin B, β-actin, and GAPDH antibodies, anti-mouse and rabbit IgG-HRP conjugated antibodies, and PKCα siRNA were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Phospho-NFκB p65 (Ser276 or Ser536) antibodies were purchased from Cell Signaling Technology (Danvers, MA). DNA constructs pTAL-SEAP and pNF-κB-SEAP were obtained from Clontech (Mountian View, CA). Expression vectors containing CMV-p65 WT, CMV-p65 276S/A and CMV-p65 536S/A were cloned in pBa5e Puro vector or pFLAG-CMV-2 vector as described previously [49]. Kinase deficient CMV-PKCα (KD-PKCα) or wild type pCMV-PKCα (WT-PKCα) were generous gifts from Alexandra C. Newton (University of California, San Diego, CA). IκBα (NFKBIA) and GAPDH primers for real-time RT-PCR were obtained from SABiosciences (Frederick, MD). A pool of mouse or human PKCα specific siRNA were purchased from Santa Cruz Biotechnology (Santa cruz, CA)."}

{"project":"GO-CC","denotations":[{"id":"T19322","span":{"begin":432,"end":436},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T19323","span":{"begin":756,"end":760},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T19324","span":{"begin":547,"end":557},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T19325","span":{"begin":600,"end":610},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T19326","span":{"begin":725,"end":735},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T19327","span":{"begin":547,"end":557},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T19328","span":{"begin":600,"end":610},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T19329","span":{"begin":725,"end":735},"obj":"http://purl.obolibrary.org/obo/GO_0042571"}],"text":"Materials\nRecombinant human M-CSF was purchased from R\u0026D Systems (Minneapolis, MN). Ro-31-8220, Gö-6976, Rotterin, and BAPTA/AM were obtained from Calbiochem (San Diego, CA). Low endotoxin RPMI and X-vivo serum free medium were obtained from Lonza Walkersville Inc (Walkersville, MD). Fetal bovine serum (FBS, certified \u003c0.06 endotoxin units/ml endotoxin levels) was purchased from Atlanta Biological (Lawrenceville, GA). All other cell culture reagents were obtained through Invitrogen (Carlsbad, CA). PKCα NF-κB p65, Lamin B, β-actin, and GAPDH antibodies, anti-mouse and rabbit IgG-HRP conjugated antibodies, and PKCα siRNA were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Phospho-NFκB p65 (Ser276 or Ser536) antibodies were purchased from Cell Signaling Technology (Danvers, MA). DNA constructs pTAL-SEAP and pNF-κB-SEAP were obtained from Clontech (Mountian View, CA). Expression vectors containing CMV-p65 WT, CMV-p65 276S/A and CMV-p65 536S/A were cloned in pBa5e Puro vector or pFLAG-CMV-2 vector as described previously [49]. Kinase deficient CMV-PKCα (KD-PKCα) or wild type pCMV-PKCα (WT-PKCα) were generous gifts from Alexandra C. Newton (University of California, San Diego, CA). IκBα (NFKBIA) and GAPDH primers for real-time RT-PCR were obtained from SABiosciences (Frederick, MD). A pool of mouse or human PKCα specific siRNA were purchased from Santa Cruz Biotechnology (Santa cruz, CA)."}

{"project":"GO-MF","denotations":[{"id":"T19305","span":{"begin":170,"end":172},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T19306","span":{"begin":498,"end":500},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T19307","span":{"begin":684,"end":686},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T19308","span":{"begin":882,"end":884},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T19309","span":{"begin":1200,"end":1202},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T19310","span":{"begin":417,"end":419},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T19311","span":{"begin":503,"end":507},"obj":"http://purl.obolibrary.org/obo/GO_0004697"},{"id":"T19312","span":{"begin":616,"end":620},"obj":"http://purl.obolibrary.org/obo/GO_0004697"},{"id":"T19313","span":{"begin":1069,"end":1073},"obj":"http://purl.obolibrary.org/obo/GO_0004697"},{"id":"T19314","span":{"begin":1078,"end":1082},"obj":"http://purl.obolibrary.org/obo/GO_0004697"},{"id":"T19315","span":{"begin":1102,"end":1106},"obj":"http://purl.obolibrary.org/obo/GO_0004697"},{"id":"T19316","span":{"begin":1111,"end":1115},"obj":"http://purl.obolibrary.org/obo/GO_0004697"},{"id":"T19317","span":{"begin":1333,"end":1337},"obj":"http://purl.obolibrary.org/obo/GO_0004697"},{"id":"T19318","span":{"begin":547,"end":557},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T19319","span":{"begin":600,"end":610},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T19320","span":{"begin":725,"end":735},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T19321","span":{"begin":1251,"end":1253},"obj":"http://purl.obolibrary.org/obo/GO_0003964"}],"text":"Materials\nRecombinant human M-CSF was purchased from R\u0026D Systems (Minneapolis, MN). Ro-31-8220, Gö-6976, Rotterin, and BAPTA/AM were obtained from Calbiochem (San Diego, CA). Low endotoxin RPMI and X-vivo serum free medium were obtained from Lonza Walkersville Inc (Walkersville, MD). Fetal bovine serum (FBS, certified \u003c0.06 endotoxin units/ml endotoxin levels) was purchased from Atlanta Biological (Lawrenceville, GA). All other cell culture reagents were obtained through Invitrogen (Carlsbad, CA). PKCα NF-κB p65, Lamin B, β-actin, and GAPDH antibodies, anti-mouse and rabbit IgG-HRP conjugated antibodies, and PKCα siRNA were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Phospho-NFκB p65 (Ser276 or Ser536) antibodies were purchased from Cell Signaling Technology (Danvers, MA). DNA constructs pTAL-SEAP and pNF-κB-SEAP were obtained from Clontech (Mountian View, CA). Expression vectors containing CMV-p65 WT, CMV-p65 276S/A and CMV-p65 536S/A were cloned in pBa5e Puro vector or pFLAG-CMV-2 vector as described previously [49]. Kinase deficient CMV-PKCα (KD-PKCα) or wild type pCMV-PKCα (WT-PKCα) were generous gifts from Alexandra C. Newton (University of California, San Diego, CA). IκBα (NFKBIA) and GAPDH primers for real-time RT-PCR were obtained from SABiosciences (Frederick, MD). A pool of mouse or human PKCα specific siRNA were purchased from Santa Cruz Biotechnology (Santa cruz, CA)."}

{"project":"GO-BP","denotations":[{"id":"T19431","span":{"begin":170,"end":172},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T19432","span":{"begin":1251,"end":1253},"obj":"http://purl.obolibrary.org/obo/GO_0003964"},{"id":"T19433","span":{"begin":498,"end":500},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T19434","span":{"begin":684,"end":686},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T19435","span":{"begin":882,"end":884},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T19436","span":{"begin":1200,"end":1202},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T19437","span":{"begin":417,"end":419},"obj":"http://purl.obolibrary.org/obo/GO_0033968"}],"text":"Materials\nRecombinant human M-CSF was purchased from R\u0026D Systems (Minneapolis, MN). Ro-31-8220, Gö-6976, Rotterin, and BAPTA/AM were obtained from Calbiochem (San Diego, CA). Low endotoxin RPMI and X-vivo serum free medium were obtained from Lonza Walkersville Inc (Walkersville, MD). Fetal bovine serum (FBS, certified \u003c0.06 endotoxin units/ml endotoxin levels) was purchased from Atlanta Biological (Lawrenceville, GA). All other cell culture reagents were obtained through Invitrogen (Carlsbad, CA). PKCα NF-κB p65, Lamin B, β-actin, and GAPDH antibodies, anti-mouse and rabbit IgG-HRP conjugated antibodies, and PKCα siRNA were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Phospho-NFκB p65 (Ser276 or Ser536) antibodies were purchased from Cell Signaling Technology (Danvers, MA). DNA constructs pTAL-SEAP and pNF-κB-SEAP were obtained from Clontech (Mountian View, CA). Expression vectors containing CMV-p65 WT, CMV-p65 276S/A and CMV-p65 536S/A were cloned in pBa5e Puro vector or pFLAG-CMV-2 vector as described previously [49]. Kinase deficient CMV-PKCα (KD-PKCα) or wild type pCMV-PKCα (WT-PKCα) were generous gifts from Alexandra C. Newton (University of California, San Diego, CA). IκBα (NFKBIA) and GAPDH primers for real-time RT-PCR were obtained from SABiosciences (Frederick, MD). A pool of mouse or human PKCα specific siRNA were purchased from Santa Cruz Biotechnology (Santa cruz, CA)."}

{"project":"UBERON-AE","denotations":[{"id":"T18718","span":{"begin":205,"end":210},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"},{"id":"T18719","span":{"begin":298,"end":303},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"text":"Materials\nRecombinant human M-CSF was purchased from R\u0026D Systems (Minneapolis, MN). Ro-31-8220, Gö-6976, Rotterin, and BAPTA/AM were obtained from Calbiochem (San Diego, CA). Low endotoxin RPMI and X-vivo serum free medium were obtained from Lonza Walkersville Inc (Walkersville, MD). Fetal bovine serum (FBS, certified \u003c0.06 endotoxin units/ml endotoxin levels) was purchased from Atlanta Biological (Lawrenceville, GA). All other cell culture reagents were obtained through Invitrogen (Carlsbad, CA). PKCα NF-κB p65, Lamin B, β-actin, and GAPDH antibodies, anti-mouse and rabbit IgG-HRP conjugated antibodies, and PKCα siRNA were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Phospho-NFκB p65 (Ser276 or Ser536) antibodies were purchased from Cell Signaling Technology (Danvers, MA). DNA constructs pTAL-SEAP and pNF-κB-SEAP were obtained from Clontech (Mountian View, CA). Expression vectors containing CMV-p65 WT, CMV-p65 276S/A and CMV-p65 536S/A were cloned in pBa5e Puro vector or pFLAG-CMV-2 vector as described previously [49]. Kinase deficient CMV-PKCα (KD-PKCα) or wild type pCMV-PKCα (WT-PKCα) were generous gifts from Alexandra C. Newton (University of California, San Diego, CA). IκBα (NFKBIA) and GAPDH primers for real-time RT-PCR were obtained from SABiosciences (Frederick, MD). A pool of mouse or human PKCα specific siRNA were purchased from Santa Cruz Biotechnology (Santa cruz, CA)."}

{"project":"pmc-enju-pas","denotations":[{"id":"T18999","span":{"begin":0,"end":9},"obj":"NN"},{"id":"T19000","span":{"begin":10,"end":21},"obj":"JJ"},{"id":"T19001","span":{"begin":22,"end":27},"obj":"JJ"},{"id":"T19002","span":{"begin":28,"end":33},"obj":"NN"},{"id":"T19003","span":{"begin":34,"end":37},"obj":"VB"},{"id":"T19004","span":{"begin":38,"end":47},"obj":"VB"},{"id":"T19005","span":{"begin":48,"end":52},"obj":"IN"},{"id":"T19006","span":{"begin":53,"end":54},"obj":"NNP"},{"id":"T19007","span":{"begin":54,"end":55},"obj":"CC"},{"id":"T19008","span":{"begin":55,"end":56},"obj":"NNP"},{"id":"T19009","span":{"begin":57,"end":64},"obj":"NNP"},{"id":"T19010","span":{"begin":65,"end":66},"obj":"-LRB-"},{"id":"T19011","span":{"begin":66,"end":77},"obj":"NNP"},{"id":"T19012","span":{"begin":77,"end":78},"obj":"-COMMA-"},{"id":"T19013","span":{"begin":79,"end":81},"obj":"NN"},{"id":"T19014","span":{"begin":81,"end":82},"obj":"-RRB-"},{"id":"T19015","span":{"begin":84,"end":94},"obj":"NN"},{"id":"T19016","span":{"begin":94,"end":95},"obj":"-COMMA-"},{"id":"T19017","span":{"begin":96,"end":103},"obj":"NN"},{"id":"T19018","span":{"begin":103,"end":104},"obj":"-COMMA-"},{"id":"T19019","span":{"begin":105,"end":113},"obj":"NN"},{"id":"T19020","span":{"begin":113,"end":114},"obj":"-COMMA-"},{"id":"T19021","span":{"begin":115,"end":118},"obj":"CC"},{"id":"T19022","span":{"begin":119,"end":127},"obj":"NN"},{"id":"T19023","span":{"begin":128,"end":132},"obj":"VB"},{"id":"T19024","span":{"begin":133,"end":141},"obj":"VB"},{"id":"T19025","span":{"begin":142,"end":146},"obj":"IN"},{"id":"T19026","span":{"begin":147,"end":157},"obj":"NN"},{"id":"T19027","span":{"begin":158,"end":159},"obj":"-LRB-"},{"id":"T19028","span":{"begin":159,"end":162},"obj":"NNP"},{"id":"T19029","span":{"begin":163,"end":168},"obj":"NNP"},{"id":"T19030","span":{"begin":168,"end":169},"obj":"-COMMA-"},{"id":"T19031","span":{"begin":170,"end":172},"obj":"NNP"},{"id":"T19032","span":{"begin":172,"end":173},"obj":"-RRB-"},{"id":"T19033","span":{"begin":175,"end":178},"obj":"JJ"},{"id":"T19034","span":{"begin":179,"end":188},"obj":"NN"},{"id":"T19035","span":{"begin":189,"end":193},"obj":"NN"},{"id":"T19036","span":{"begin":194,"end":197},"obj":"CC"},{"id":"T19037","span":{"begin":198,"end":204},"obj":"FW"},{"id":"T19038","span":{"begin":205,"end":210},"obj":"NN"},{"id":"T19039","span":{"begin":211,"end":215},"obj":"JJ"},{"id":"T19040","span":{"begin":216,"end":222},"obj":"NN"},{"id":"T19041","span":{"begin":223,"end":227},"obj":"VB"},{"id":"T19042","span":{"begin":228,"end":236},"obj":"VB"},{"id":"T19043","span":{"begin":237,"end":241},"obj":"IN"},{"id":"T19044","span":{"begin":242,"end":247},"obj":"NNP"},{"id":"T19045","span":{"begin":248,"end":260},"obj":"NNP"},{"id":"T19046","span":{"begin":261,"end":264},"obj":"NNP"},{"id":"T19047","span":{"begin":265,"end":266},"obj":"-LRB-"},{"id":"T19048","span":{"begin":266,"end":278},"obj":"NNP"},{"id":"T19049","span":{"begin":278,"end":279},"obj":"-COMMA-"},{"id":"T19050","span":{"begin":280,"end":282},"obj":"NNP"},{"id":"T19051","span":{"begin":282,"end":283},"obj":"-RRB-"},{"id":"T19052","span":{"begin":285,"end":290},"obj":"JJ"},{"id":"T19053","span":{"begin":291,"end":297},"obj":"JJ"},{"id":"T19054","span":{"begin":298,"end":303},"obj":"NN"},{"id":"T19055","span":{"begin":304,"end":305},"obj":"-LRB-"},{"id":"T19056","span":{"begin":305,"end":308},"obj":"NNP"},{"id":"T19057","span":{"begin":308,"end":309},"obj":"-COMMA-"},{"id":"T19058","span":{"begin":310,"end":319},"obj":"VB"},{"id":"T19059","span":{"begin":320,"end":321},"obj":"SYM"},{"id":"T19060","span":{"begin":321,"end":325},"obj":"CD"},{"id":"T19061","span":{"begin":326,"end":335},"obj":"NN"},{"id":"T19062","span":{"begin":336,"end":344},"obj":"NN"},{"id":"T19063","span":{"begin":345,"end":354},"obj":"NN"},{"id":"T19064","span":{"begin":355,"end":361},"obj":"NN"},{"id":"T19065","span":{"begin":361,"end":362},"obj":"-RRB-"},{"id":"T19066","span":{"begin":363,"end":366},"obj":"VB"},{"id":"T19067","span":{"begin":367,"end":376},"obj":"VB"},{"id":"T19068","span":{"begin":377,"end":381},"obj":"IN"},{"id":"T19069","span":{"begin":382,"end":389},"obj":"NNP"},{"id":"T19070","span":{"begin":390,"end":400},"obj":"NNP"},{"id":"T19071","span":{"begin":401,"end":402},"obj":"-LRB-"},{"id":"T19072","span":{"begin":402,"end":415},"obj":"NNP"},{"id":"T19073","span":{"begin":415,"end":416},"obj":"-COMMA-"},{"id":"T19074","span":{"begin":417,"end":419},"obj":"NN"},{"id":"T19075","span":{"begin":419,"end":420},"obj":"-RRB-"},{"id":"T19076","span":{"begin":422,"end":425},"obj":"DT"},{"id":"T19077","span":{"begin":426,"end":431},"obj":"JJ"},{"id":"T19078","span":{"begin":432,"end":436},"obj":"NN"},{"id":"T19079","span":{"begin":437,"end":444},"obj":"NN"},{"id":"T19080","span":{"begin":445,"end":453},"obj":"NN"},{"id":"T19081","span":{"begin":454,"end":458},"obj":"VB"},{"id":"T19082","span":{"begin":459,"end":467},"obj":"VB"},{"id":"T19083","span":{"begin":468,"end":475},"obj":"IN"},{"id":"T19084","span":{"begin":476,"end":486},"obj":"NN"},{"id":"T19085","span":{"begin":487,"end":488},"obj":"-LRB-"},{"id":"T19086","span":{"begin":488,"end":496},"obj":"NNP"},{"id":"T19087","span":{"begin":496,"end":497},"obj":"-COMMA-"},{"id":"T19088","span":{"begin":498,"end":500},"obj":"NN"},{"id":"T19089","span":{"begin":500,"end":501},"obj":"-RRB-"},{"id":"T19090","span":{"begin":503,"end":507},"obj":"NN"},{"id":"T19091","span":{"begin":508,"end":513},"obj":"NN"},{"id":"T19092","span":{"begin":514,"end":517},"obj":"NN"},{"id":"T19093","span":{"begin":517,"end":518},"obj":"-COMMA-"},{"id":"T19094","span":{"begin":519,"end":524},"obj":"NNP"},{"id":"T19095","span":{"begin":525,"end":526},"obj":"NNP"},{"id":"T19096","span":{"begin":526,"end":527},"obj":"-COMMA-"},{"id":"T19097","span":{"begin":528,"end":535},"obj":"NN"},{"id":"T19098","span":{"begin":535,"end":536},"obj":"-COMMA-"},{"id":"T19099","span":{"begin":537,"end":540},"obj":"CC"},{"id":"T19100","span":{"begin":541,"end":546},"obj":"NN"},{"id":"T19101","span":{"begin":547,"end":557},"obj":"NN"},{"id":"T19102","span":{"begin":557,"end":558},"obj":"-COMMA-"},{"id":"T19103","span":{"begin":559,"end":569},"obj":"JJ"},{"id":"T19104","span":{"begin":570,"end":573},"obj":"CC"},{"id":"T19105","span":{"begin":574,"end":580},"obj":"NN"},{"id":"T19106","span":{"begin":581,"end":588},"obj":"NN"},{"id":"T19107","span":{"begin":589,"end":599},"obj":"VB"},{"id":"T19108","span":{"begin":600,"end":610},"obj":"NN"},{"id":"T19109","span":{"begin":610,"end":611},"obj":"-COMMA-"},{"id":"T19110","span":{"begin":612,"end":615},"obj":"CC"},{"id":"T19111","span":{"begin":616,"end":620},"obj":"NN"},{"id":"T19112","span":{"begin":621,"end":626},"obj":"NN"},{"id":"T19113","span":{"begin":627,"end":631},"obj":"VB"},{"id":"T19114","span":{"begin":632,"end":640},"obj":"VB"},{"id":"T19115","span":{"begin":641,"end":645},"obj":"IN"},{"id":"T19116","span":{"begin":646,"end":651},"obj":"NNP"},{"id":"T19117","span":{"begin":652,"end":656},"obj":"NNP"},{"id":"T19118","span":{"begin":657,"end":670},"obj":"NNP"},{"id":"T19119","span":{"begin":671,"end":672},"obj":"-LRB-"},{"id":"T19120","span":{"begin":672,"end":677},"obj":"NNP"},{"id":"T19121","span":{"begin":678,"end":682},"obj":"NNP"},{"id":"T19122","span":{"begin":682,"end":683},"obj":"-COMMA-"},{"id":"T19123","span":{"begin":684,"end":686},"obj":"NNP"},{"id":"T19124","span":{"begin":686,"end":687},"obj":"-RRB-"},{"id":"T19125","span":{"begin":689,"end":701},"obj":"NN"},{"id":"T19126","span":{"begin":702,"end":705},"obj":"NN"},{"id":"T19127","span":{"begin":706,"end":707},"obj":"-LRB-"},{"id":"T19128","span":{"begin":707,"end":713},"obj":"NN"},{"id":"T19129","span":{"begin":714,"end":716},"obj":"CC"},{"id":"T19130","span":{"begin":717,"end":723},"obj":"NN"},{"id":"T19131","span":{"begin":723,"end":724},"obj":"-RRB-"},{"id":"T19132","span":{"begin":725,"end":735},"obj":"NN"},{"id":"T19133","span":{"begin":736,"end":740},"obj":"VB"},{"id":"T19134","span":{"begin":741,"end":750},"obj":"VB"},{"id":"T19135","span":{"begin":751,"end":755},"obj":"IN"},{"id":"T19136","span":{"begin":756,"end":760},"obj":"NNP"},{"id":"T19137","span":{"begin":761,"end":770},"obj":"NNP"},{"id":"T19138","span":{"begin":771,"end":781},"obj":"NNP"},{"id":"T19139","span":{"begin":782,"end":783},"obj":"-LRB-"},{"id":"T19140","span":{"begin":783,"end":790},"obj":"NNP"},{"id":"T19141","span":{"begin":790,"end":791},"obj":"-COMMA-"},{"id":"T19142","span":{"begin":792,"end":794},"obj":"JJ"},{"id":"T19143","span":{"begin":794,"end":795},"obj":"-RRB-"},{"id":"T19144","span":{"begin":797,"end":800},"obj":"NN"},{"id":"T19145","span":{"begin":801,"end":811},"obj":"NN"},{"id":"T19146","span":{"begin":812,"end":821},"obj":"NN"},{"id":"T19147","span":{"begin":822,"end":825},"obj":"CC"},{"id":"T19148","span":{"begin":826,"end":837},"obj":"NN"},{"id":"T19149","span":{"begin":838,"end":842},"obj":"VB"},{"id":"T19150","span":{"begin":843,"end":851},"obj":"VB"},{"id":"T19151","span":{"begin":852,"end":856},"obj":"IN"},{"id":"T19152","span":{"begin":857,"end":865},"obj":"NN"},{"id":"T19153","span":{"begin":866,"end":867},"obj":"-LRB-"},{"id":"T19154","span":{"begin":867,"end":875},"obj":"NNP"},{"id":"T19155","span":{"begin":876,"end":880},"obj":"NNP"},{"id":"T19156","span":{"begin":880,"end":881},"obj":"-COMMA-"},{"id":"T19157","span":{"begin":882,"end":884},"obj":"NNP"},{"id":"T19158","span":{"begin":884,"end":885},"obj":"-RRB-"},{"id":"T19159","span":{"begin":887,"end":897},"obj":"NN"},{"id":"T19160","span":{"begin":898,"end":905},"obj":"NN"},{"id":"T19161","span":{"begin":906,"end":916},"obj":"VB"},{"id":"T19162","span":{"begin":917,"end":924},"obj":"NN"},{"id":"T19163","span":{"begin":925,"end":927},"obj":"NN"},{"id":"T19164","span":{"begin":927,"end":928},"obj":"-COMMA-"},{"id":"T19165","span":{"begin":929,"end":936},"obj":"NN"},{"id":"T19166","span":{"begin":937,"end":943},"obj":"NN"},{"id":"T19167","span":{"begin":944,"end":947},"obj":"CC"},{"id":"T19168","span":{"begin":948,"end":955},"obj":"NN"},{"id":"T19169","span":{"begin":956,"end":962},"obj":"NN"},{"id":"T19170","span":{"begin":963,"end":967},"obj":"VB"},{"id":"T19171","span":{"begin":968,"end":974},"obj":"VB"},{"id":"T19172","span":{"begin":975,"end":977},"obj":"IN"},{"id":"T19173","span":{"begin":978,"end":983},"obj":"NN"},{"id":"T19174","span":{"begin":984,"end":988},"obj":"NN"},{"id":"T19175","span":{"begin":989,"end":995},"obj":"NN"},{"id":"T19176","span":{"begin":996,"end":998},"obj":"CC"},{"id":"T19177","span":{"begin":999,"end":1010},"obj":"NN"},{"id":"T19178","span":{"begin":1011,"end":1017},"obj":"NN"},{"id":"T19179","span":{"begin":1018,"end":1020},"obj":"IN"},{"id":"T19180","span":{"begin":1021,"end":1030},"obj":"VB"},{"id":"T19181","span":{"begin":1031,"end":1041},"obj":"RB"},{"id":"T19182","span":{"begin":1042,"end":1043},"obj":"-LRB-"},{"id":"T19183","span":{"begin":1043,"end":1045},"obj":"CD"},{"id":"T19184","span":{"begin":1045,"end":1046},"obj":"-RRB-"},{"id":"T19185","span":{"begin":1048,"end":1054},"obj":"NN"},{"id":"T19186","span":{"begin":1055,"end":1064},"obj":"JJ"},{"id":"T19187","span":{"begin":1065,"end":1073},"obj":"NN"},{"id":"T19188","span":{"begin":1074,"end":1075},"obj":"-LRB-"},{"id":"T19189","span":{"begin":1075,"end":1082},"obj":"NN"},{"id":"T19190","span":{"begin":1082,"end":1083},"obj":"-RRB-"},{"id":"T19191","span":{"begin":1084,"end":1086},"obj":"CC"},{"id":"T19192","span":{"begin":1087,"end":1091},"obj":"JJ"},{"id":"T19193","span":{"begin":1092,"end":1096},"obj":"NN"},{"id":"T19194","span":{"begin":1097,"end":1106},"obj":"NN"},{"id":"T19195","span":{"begin":1107,"end":1108},"obj":"-LRB-"},{"id":"T19196","span":{"begin":1108,"end":1115},"obj":"NN"},{"id":"T19197","span":{"begin":1115,"end":1116},"obj":"-RRB-"},{"id":"T19198","span":{"begin":1117,"end":1121},"obj":"VB"},{"id":"T19199","span":{"begin":1122,"end":1130},"obj":"JJ"},{"id":"T19200","span":{"begin":1131,"end":1136},"obj":"NN"},{"id":"T19201","span":{"begin":1137,"end":1141},"obj":"IN"},{"id":"T19202","span":{"begin":1142,"end":1151},"obj":"NNP"},{"id":"T19203","span":{"begin":1152,"end":1154},"obj":"NNP"},{"id":"T19204","span":{"begin":1155,"end":1161},"obj":"NNP"},{"id":"T19205","span":{"begin":1162,"end":1163},"obj":"-LRB-"},{"id":"T19206","span":{"begin":1163,"end":1173},"obj":"NNP"},{"id":"T19207","span":{"begin":1174,"end":1176},"obj":"IN"},{"id":"T19208","span":{"begin":1177,"end":1187},"obj":"NNP"},{"id":"T19209","span":{"begin":1187,"end":1188},"obj":"-COMMA-"},{"id":"T19210","span":{"begin":1189,"end":1192},"obj":"NNP"},{"id":"T19211","span":{"begin":1193,"end":1198},"obj":"NNP"},{"id":"T19212","span":{"begin":1198,"end":1199},"obj":"-COMMA-"},{"id":"T19213","span":{"begin":1200,"end":1202},"obj":"NNP"},{"id":"T19214","span":{"begin":1202,"end":1203},"obj":"-RRB-"},{"id":"T19215","span":{"begin":1205,"end":1209},"obj":"NN"},{"id":"T19216","span":{"begin":1210,"end":1211},"obj":"-LRB-"},{"id":"T19217","span":{"begin":1211,"end":1217},"obj":"NN"},{"id":"T19218","span":{"begin":1217,"end":1218},"obj":"-RRB-"},{"id":"T19219","span":{"begin":1219,"end":1222},"obj":"CC"},{"id":"T19220","span":{"begin":1223,"end":1228},"obj":"NN"},{"id":"T19221","span":{"begin":1229,"end":1236},"obj":"NN"},{"id":"T19222","span":{"begin":1237,"end":1240},"obj":"IN"},{"id":"T19223","span":{"begin":1241,"end":1250},"obj":"JJ"},{"id":"T19224","span":{"begin":1251,"end":1257},"obj":"NN"},{"id":"T19225","span":{"begin":1258,"end":1262},"obj":"VB"},{"id":"T19226","span":{"begin":1263,"end":1271},"obj":"VB"},{"id":"T19227","span":{"begin":1272,"end":1276},"obj":"IN"},{"id":"T19228","span":{"begin":1277,"end":1290},"obj":"NNP"},{"id":"T19229","span":{"begin":1291,"end":1292},"obj":"-LRB-"},{"id":"T19230","span":{"begin":1292,"end":1301},"obj":"NNP"},{"id":"T19231","span":{"begin":1301,"end":1302},"obj":"-COMMA-"},{"id":"T19232","span":{"begin":1303,"end":1305},"obj":"NNP"},{"id":"T19233","span":{"begin":1305,"end":1306},"obj":"-RRB-"},{"id":"T19234","span":{"begin":1308,"end":1309},"obj":"DT"},{"id":"T19235","span":{"begin":1310,"end":1314},"obj":"NN"},{"id":"T19236","span":{"begin":1315,"end":1317},"obj":"IN"},{"id":"T19237","span":{"begin":1318,"end":1323},"obj":"NN"},{"id":"T19238","span":{"begin":1324,"end":1326},"obj":"CC"},{"id":"T19239","span":{"begin":1327,"end":1332},"obj":"JJ"},{"id":"T19240","span":{"begin":1333,"end":1337},"obj":"NN"},{"id":"T19241","span":{"begin":1338,"end":1346},"obj":"JJ"},{"id":"T19242","span":{"begin":1347,"end":1352},"obj":"NN"},{"id":"T19243","span":{"begin":1353,"end":1357},"obj":"VB"},{"id":"T19244","span":{"begin":1358,"end":1367},"obj":"VB"},{"id":"T19245","span":{"begin":1368,"end":1372},"obj":"IN"},{"id":"T19246","span":{"begin":1373,"end":1378},"obj":"NNP"},{"id":"T19247","span":{"begin":1379,"end":1383},"obj":"NNP"},{"id":"T19248","span":{"begin":1384,"end":1397},"obj":"NNP"},{"id":"T19249","span":{"begin":1398,"end":1399},"obj":"-LRB-"},{"id":"T19250","span":{"begin":1399,"end":1404},"obj":"NNP"},{"id":"T19251","span":{"begin":1405,"end":1409},"obj":"NN"},{"id":"T19252","span":{"begin":1409,"end":1410},"obj":"-COMMA-"},{"id":"T19253","span":{"begin":1411,"end":1413},"obj":"NN"},{"id":"T19254","span":{"begin":1413,"end":1414},"obj":"-RRB-"}],"relations":[{"id":"R14200","pred":"arg1Of","subj":"T19002","obj":"T19000"},{"id":"R14201","pred":"arg1Of","subj":"T19002","obj":"T19001"},{"id":"R14202","pred":"arg1Of","subj":"T19002","obj":"T19003"},{"id":"R14203","pred":"arg2Of","subj":"T19002","obj":"T19004"},{"id":"R14204","pred":"arg2Of","subj":"T19004","obj":"T19003"},{"id":"R14205","pred":"arg1Of","subj":"T19004","obj":"T19005"},{"id":"R14206","pred":"arg2Of","subj":"T19009","obj":"T19005"},{"id":"R14207","pred":"arg1Of","subj":"T19009","obj":"T19006"},{"id":"R14208","pred":"arg1Of","subj":"T19009","obj":"T19007"},{"id":"R14209","pred":"arg1Of","subj":"T19009","obj":"T19008"},{"id":"R14210","pred":"arg1Of","subj":"T19009","obj":"T19010"},{"id":"R14211","pred":"arg2Of","subj":"T19011","obj":"T19010"},{"id":"R14212","pred":"arg1Of","subj":"T19011","obj":"T19012"},{"id":"R14213","pred":"arg1Of","subj":"T19011","obj":"T19013"},{"id":"R14214","pred":"arg3Of","subj":"T19014","obj":"T19010"},{"id":"R14215","pred":"arg1Of","subj":"T19015","obj":"T19016"},{"id":"R14216","pred":"arg1Of","subj":"T19016","obj":"T19018"},{"id":"R14217","pred":"arg2Of","subj":"T19017","obj":"T19016"},{"id":"R14218","pred":"arg1Of","subj":"T19018","obj":"T19021"},{"id":"R14219","pred":"arg2Of","subj":"T19019","obj":"T19018"},{"id":"R14220","pred":"arg1Of","subj":"T19021","obj":"T19020"},{"id":"R14221","pred":"arg1Of","subj":"T19021","obj":"T19023"},{"id":"R14222","pred":"arg2Of","subj":"T19021","obj":"T19024"},{"id":"R14223","pred":"arg2Of","subj":"T19022","obj":"T19021"},{"id":"R14224","pred":"arg2Of","subj":"T19024","obj":"T19023"},{"id":"R14225","pred":"arg1Of","subj":"T19024","obj":"T19025"},{"id":"R14226","pred":"arg2Of","subj":"T19026","obj":"T19025"},{"id":"R14227","pred":"arg1Of","subj":"T19026","obj":"T19027"},{"id":"R14228","pred":"arg2Of","subj":"T19029","obj":"T19027"},{"id":"R14229","pred":"arg1Of","subj":"T19029","obj":"T19028"},{"id":"R14230","pred":"arg1Of","subj":"T19029","obj":"T19030"},{"id":"R14231","pred":"arg2Of","subj":"T19031","obj":"T19030"},{"id":"R14232","pred":"arg3Of","subj":"T19032","obj":"T19027"},{"id":"R14233","pred":"arg1Of","subj":"T19035","obj":"T19033"},{"id":"R14234","pred":"arg1Of","subj":"T19035","obj":"T19034"},{"id":"R14235","pred":"arg1Of","subj":"T19035","obj":"T19036"},{"id":"R14236","pred":"arg1Of","subj":"T19036","obj":"T19041"},{"id":"R14237","pred":"arg2Of","subj":"T19036","obj":"T19042"},{"id":"R14238","pred":"arg2Of","subj":"T19040","obj":"T19036"},{"id":"R14239","pred":"arg1Of","subj":"T19040","obj":"T19037"},{"id":"R14240","pred":"arg1Of","subj":"T19040","obj":"T19038"},{"id":"R14241","pred":"arg1Of","subj":"T19040","obj":"T19039"},{"id":"R14242","pred":"arg2Of","subj":"T19042","obj":"T19041"},{"id":"R14243","pred":"arg1Of","subj":"T19042","obj":"T19043"},{"id":"R14244","pred":"arg2Of","subj":"T19046","obj":"T19043"},{"id":"R14245","pred":"arg1Of","subj":"T19046","obj":"T19044"},{"id":"R14246","pred":"arg1Of","subj":"T19046","obj":"T19045"},{"id":"R14247","pred":"arg1Of","subj":"T19046","obj":"T19047"},{"id":"R14248","pred":"arg2Of","subj":"T19048","obj":"T19047"},{"id":"R14249","pred":"arg1Of","subj":"T19048","obj":"T19049"},{"id":"R14250","pred":"arg1Of","subj":"T19048","obj":"T19050"},{"id":"R14251","pred":"arg3Of","subj":"T19051","obj":"T19047"},{"id":"R14252","pred":"arg1Of","subj":"T19054","obj":"T19052"},{"id":"R14253","pred":"arg1Of","subj":"T19054","obj":"T19053"},{"id":"R14254","pred":"arg1Of","subj":"T19054","obj":"T19055"},{"id":"R14255","pred":"arg1Of","subj":"T19054","obj":"T19066"},{"id":"R14256","pred":"arg2Of","subj":"T19054","obj":"T19067"},{"id":"R14257","pred":"arg2Of","subj":"T19056","obj":"T19055"},{"id":"R14258","pred":"arg2Of","subj":"T19056","obj":"T19058"},{"id":"R14259","pred":"arg1Of","subj":"T19058","obj":"T19057"},{"id":"R14260","pred":"arg1Of","subj":"T19060","obj":"T19059"},{"id":"R14261","pred":"arg3Of","subj":"T19064","obj":"T19058"},{"id":"R14262","pred":"arg1Of","subj":"T19064","obj":"T19060"},{"id":"R14263","pred":"arg1Of","subj":"T19064","obj":"T19061"},{"id":"R14264","pred":"arg1Of","subj":"T19064","obj":"T19062"},{"id":"R14265","pred":"arg1Of","subj":"T19064","obj":"T19063"},{"id":"R14266","pred":"arg3Of","subj":"T19065","obj":"T19055"},{"id":"R14267","pred":"arg2Of","subj":"T19067","obj":"T19066"},{"id":"R14268","pred":"arg1Of","subj":"T19067","obj":"T19068"},{"id":"R14269","pred":"arg2Of","subj":"T19070","obj":"T19068"},{"id":"R14270","pred":"arg1Of","subj":"T19070","obj":"T19069"},{"id":"R14271","pred":"arg1Of","subj":"T19070","obj":"T19071"},{"id":"R14272","pred":"arg2Of","subj":"T19072","obj":"T19071"},{"id":"R14273","pred":"arg1Of","subj":"T19072","obj":"T19073"},{"id":"R14274","pred":"arg2Of","subj":"T19074","obj":"T19073"},{"id":"R14275","pred":"arg3Of","subj":"T19075","obj":"T19071"},{"id":"R14276","pred":"arg1Of","subj":"T19080","obj":"T19076"},{"id":"R14277","pred":"arg1Of","subj":"T19080","obj":"T19077"},{"id":"R14278","pred":"arg1Of","subj":"T19080","obj":"T19078"},{"id":"R14279","pred":"arg1Of","subj":"T19080","obj":"T19079"},{"id":"R14280","pred":"arg1Of","subj":"T19080","obj":"T19081"},{"id":"R14281","pred":"arg2Of","subj":"T19080","obj":"T19082"},{"id":"R14282","pred":"arg2Of","subj":"T19082","obj":"T19081"},{"id":"R14283","pred":"arg1Of","subj":"T19082","obj":"T19083"},{"id":"R14284","pred":"arg1Of","subj":"T19082","obj":"T19085"},{"id":"R14285","pred":"arg2Of","subj":"T19084","obj":"T19083"},{"id":"R14286","pred":"arg2Of","subj":"T19086","obj":"T19085"},{"id":"R14287","pred":"arg1Of","subj":"T19086","obj":"T19087"},{"id":"R14288","pred":"arg1Of","subj":"T19086","obj":"T19088"},{"id":"R14289","pred":"arg3Of","subj":"T19089","obj":"T19085"},{"id":"R14290","pred":"arg1Of","subj":"T19092","obj":"T19090"},{"id":"R14291","pred":"arg1Of","subj":"T19092","obj":"T19091"},{"id":"R14292","pred":"arg1Of","subj":"T19092","obj":"T19093"},{"id":"R14293","pred":"arg1Of","subj":"T19092","obj":"T19099"},{"id":"R14294","pred":"arg2Of","subj":"T19095","obj":"T19093"},{"id":"R14295","pred":"arg1Of","subj":"T19095","obj":"T19094"},{"id":"R14296","pred":"arg1Of","subj":"T19095","obj":"T19096"},{"id":"R14297","pred":"arg2Of","subj":"T19097","obj":"T19096"},{"id":"R14298","pred":"arg1Of","subj":"T19099","obj":"T19098"},{"id":"R14299","pred":"arg1Of","subj":"T19099","obj":"T19107"},{"id":"R14300","pred":"arg1Of","subj":"T19101","obj":"T19100"},{"id":"R14301","pred":"arg1Of","subj":"T19101","obj":"T19102"},{"id":"R14302","pred":"arg1Of","subj":"T19101","obj":"T19103"},{"id":"R14303","pred":"arg1Of","subj":"T19101","obj":"T19104"},{"id":"R14304","pred":"arg2Of","subj":"T19104","obj":"T19099"},{"id":"R14305","pred":"arg2Of","subj":"T19106","obj":"T19104"},{"id":"R14306","pred":"arg1Of","subj":"T19106","obj":"T19105"},{"id":"R14307","pred":"arg1Of","subj":"T19107","obj":"T19110"},{"id":"R14308","pred":"arg2Of","subj":"T19108","obj":"T19107"},{"id":"R14309","pred":"arg1Of","subj":"T19110","obj":"T19109"},{"id":"R14310","pred":"arg1Of","subj":"T19112","obj":"T19111"},{"id":"R14311","pred":"arg1Of","subj":"T19112","obj":"T19113"},{"id":"R14312","pred":"arg2Of","subj":"T19112","obj":"T19114"},{"id":"R14313","pred":"arg2Of","subj":"T19114","obj":"T19110"},{"id":"R14314","pred":"arg2Of","subj":"T19114","obj":"T19113"},{"id":"R14315","pred":"arg1Of","subj":"T19114","obj":"T19115"},{"id":"R14316","pred":"arg2Of","subj":"T19118","obj":"T19115"},{"id":"R14317","pred":"arg1Of","subj":"T19118","obj":"T19116"},{"id":"R14318","pred":"arg1Of","subj":"T19118","obj":"T19117"},{"id":"R14319","pred":"arg1Of","subj":"T19118","obj":"T19119"},{"id":"R14320","pred":"arg2Of","subj":"T19121","obj":"T19119"},{"id":"R14321","pred":"arg1Of","subj":"T19121","obj":"T19120"},{"id":"R14322","pred":"arg1Of","subj":"T19121","obj":"T19122"},{"id":"R14323","pred":"arg2Of","subj":"T19123","obj":"T19122"},{"id":"R14324","pred":"arg3Of","subj":"T19124","obj":"T19119"},{"id":"R14325","pred":"arg1Of","subj":"T19128","obj":"T19129"},{"id":"R14326","pred":"arg2Of","subj":"T19129","obj":"T19127"},{"id":"R14327","pred":"arg2Of","subj":"T19130","obj":"T19129"},{"id":"R14328","pred":"arg3Of","subj":"T19131","obj":"T19127"},{"id":"R14329","pred":"arg1Of","subj":"T19132","obj":"T19125"},{"id":"R14330","pred":"arg1Of","subj":"T19132","obj":"T19126"},{"id":"R14331","pred":"arg1Of","subj":"T19132","obj":"T19127"},{"id":"R14332","pred":"arg1Of","subj":"T19132","obj":"T19133"},{"id":"R14333","pred":"arg2Of","subj":"T19132","obj":"T19134"},{"id":"R14334","pred":"arg2Of","subj":"T19134","obj":"T19133"},{"id":"R14335","pred":"arg1Of","subj":"T19134","obj":"T19135"},{"id":"R14336","pred":"arg2Of","subj":"T19138","obj":"T19135"},{"id":"R14337","pred":"arg1Of","subj":"T19138","obj":"T19136"},{"id":"R14338","pred":"arg1Of","subj":"T19138","obj":"T19137"},{"id":"R14339","pred":"arg1Of","subj":"T19138","obj":"T19139"},{"id":"R14340","pred":"arg2Of","subj":"T19140","obj":"T19139"},{"id":"R14341","pred":"arg1Of","subj":"T19140","obj":"T19141"},{"id":"R14342","pred":"arg1Of","subj":"T19140","obj":"T19142"},{"id":"R14343","pred":"arg3Of","subj":"T19143","obj":"T19139"},{"id":"R14344","pred":"arg1Of","subj":"T19146","obj":"T19147"},{"id":"R14345","pred":"arg1Of","subj":"T19147","obj":"T19144"},{"id":"R14346","pred":"arg1Of","subj":"T19147","obj":"T19145"},{"id":"R14347","pred":"arg1Of","subj":"T19147","obj":"T19149"},{"id":"R14348","pred":"arg2Of","subj":"T19147","obj":"T19150"},{"id":"R14349","pred":"arg2Of","subj":"T19148","obj":"T19147"},{"id":"R14350","pred":"arg2Of","subj":"T19150","obj":"T19149"},{"id":"R14351","pred":"arg1Of","subj":"T19150","obj":"T19151"},{"id":"R14352","pred":"arg2Of","subj":"T19152","obj":"T19151"},{"id":"R14353","pred":"arg1Of","subj":"T19152","obj":"T19153"},{"id":"R14354","pred":"arg2Of","subj":"T19155","obj":"T19153"},{"id":"R14355","pred":"arg1Of","subj":"T19155","obj":"T19154"},{"id":"R14356","pred":"arg1Of","subj":"T19155","obj":"T19156"},{"id":"R14357","pred":"arg2Of","subj":"T19157","obj":"T19156"},{"id":"R14358","pred":"arg3Of","subj":"T19158","obj":"T19153"},{"id":"R14359","pred":"arg1Of","subj":"T19160","obj":"T19159"},{"id":"R14360","pred":"arg1Of","subj":"T19160","obj":"T19161"},{"id":"R14361","pred":"arg1Of","subj":"T19160","obj":"T19170"},{"id":"R14362","pred":"arg2Of","subj":"T19160","obj":"T19171"},{"id":"R14363","pred":"arg1Of","subj":"T19163","obj":"T19162"},{"id":"R14364","pred":"arg1Of","subj":"T19163","obj":"T19164"},{"id":"R14365","pred":"arg1Of","subj":"T19164","obj":"T19167"},{"id":"R14366","pred":"arg2Of","subj":"T19166","obj":"T19164"},{"id":"R14367","pred":"arg1Of","subj":"T19166","obj":"T19165"},{"id":"R14368","pred":"arg2Of","subj":"T19167","obj":"T19161"},{"id":"R14369","pred":"arg2Of","subj":"T19169","obj":"T19167"},{"id":"R14370","pred":"arg1Of","subj":"T19169","obj":"T19168"},{"id":"R14371","pred":"arg2Of","subj":"T19171","obj":"T19170"},{"id":"R14372","pred":"arg1Of","subj":"T19171","obj":"T19172"},{"id":"R14373","pred":"arg1Of","subj":"T19175","obj":"T19173"},{"id":"R14374","pred":"arg1Of","subj":"T19175","obj":"T19174"},{"id":"R14375","pred":"arg1Of","subj":"T19175","obj":"T19176"},{"id":"R14376","pred":"arg2Of","subj":"T19176","obj":"T19172"},{"id":"R14377","pred":"arg1Of","subj":"T19176","obj":"T19179"},{"id":"R14378","pred":"arg2Of","subj":"T19178","obj":"T19176"},{"id":"R14379","pred":"arg1Of","subj":"T19178","obj":"T19177"},{"id":"R14380","pred":"arg2Of","subj":"T19180","obj":"T19179"},{"id":"R14381","pred":"arg1Of","subj":"T19180","obj":"T19181"},{"id":"R14382","pred":"arg1Of","subj":"T19180","obj":"T19182"},{"id":"R14383","pred":"arg2Of","subj":"T19183","obj":"T19182"},{"id":"R14384","pred":"arg3Of","subj":"T19184","obj":"T19182"},{"id":"R14385","pred":"arg1Of","subj":"T19187","obj":"T19185"},{"id":"R14386","pred":"arg1Of","subj":"T19187","obj":"T19186"},{"id":"R14387","pred":"arg1Of","subj":"T19187","obj":"T19188"},{"id":"R14388","pred":"arg1Of","subj":"T19187","obj":"T19191"},{"id":"R14389","pred":"arg2Of","subj":"T19189","obj":"T19188"},{"id":"R14390","pred":"arg3Of","subj":"T19190","obj":"T19188"},{"id":"R14391","pred":"arg1Of","subj":"T19191","obj":"T19198"},{"id":"R14392","pred":"arg2Of","subj":"T19194","obj":"T19191"},{"id":"R14393","pred":"arg1Of","subj":"T19194","obj":"T19192"},{"id":"R14394","pred":"arg1Of","subj":"T19194","obj":"T19193"},{"id":"R14395","pred":"arg1Of","subj":"T19194","obj":"T19195"},{"id":"R14396","pred":"arg2Of","subj":"T19196","obj":"T19195"},{"id":"R14397","pred":"arg3Of","subj":"T19197","obj":"T19195"},{"id":"R14398","pred":"arg2Of","subj":"T19200","obj":"T19198"},{"id":"R14399","pred":"arg1Of","subj":"T19200","obj":"T19199"},{"id":"R14400","pred":"arg1Of","subj":"T19200","obj":"T19201"},{"id":"R14401","pred":"arg1Of","subj":"T19200","obj":"T19205"},{"id":"R14402","pred":"arg2Of","subj":"T19204","obj":"T19201"},{"id":"R14403","pred":"arg1Of","subj":"T19204","obj":"T19202"},{"id":"R14404","pred":"arg1Of","subj":"T19204","obj":"T19203"},{"id":"R14405","pred":"arg2Of","subj":"T19206","obj":"T19205"},{"id":"R14406","pred":"arg1Of","subj":"T19206","obj":"T19207"},{"id":"R14407","pred":"arg1Of","subj":"T19206","obj":"T19209"},{"id":"R14408","pred":"arg2Of","subj":"T19208","obj":"T19207"},{"id":"R14409","pred":"arg2Of","subj":"T19211","obj":"T19209"},{"id":"R14410","pred":"arg1Of","subj":"T19211","obj":"T19210"},{"id":"R14411","pred":"arg1Of","subj":"T19211","obj":"T19212"},{"id":"R14412","pred":"arg2Of","subj":"T19213","obj":"T19212"},{"id":"R14413","pred":"arg3Of","subj":"T19214","obj":"T19205"},{"id":"R14414","pred":"arg1Of","subj":"T19215","obj":"T19216"},{"id":"R14415","pred":"arg1Of","subj":"T19215","obj":"T19219"},{"id":"R14416","pred":"arg2Of","subj":"T19217","obj":"T19216"},{"id":"R14417","pred":"arg3Of","subj":"T19218","obj":"T19216"},{"id":"R14418","pred":"arg1Of","subj":"T19219","obj":"T19222"},{"id":"R14419","pred":"arg1Of","subj":"T19219","obj":"T19225"},{"id":"R14420","pred":"arg2Of","subj":"T19219","obj":"T19226"},{"id":"R14421","pred":"arg2Of","subj":"T19221","obj":"T19219"},{"id":"R14422","pred":"arg1Of","subj":"T19221","obj":"T19220"},{"id":"R14423","pred":"arg2Of","subj":"T19224","obj":"T19222"},{"id":"R14424","pred":"arg1Of","subj":"T19224","obj":"T19223"},{"id":"R14425","pred":"arg2Of","subj":"T19226","obj":"T19225"},{"id":"R14426","pred":"arg1Of","subj":"T19226","obj":"T19227"},{"id":"R14427","pred":"arg2Of","subj":"T19228","obj":"T19227"},{"id":"R14428","pred":"arg1Of","subj":"T19228","obj":"T19229"},{"id":"R14429","pred":"arg2Of","subj":"T19230","obj":"T19229"},{"id":"R14430","pred":"arg1Of","subj":"T19230","obj":"T19231"},{"id":"R14431","pred":"arg1Of","subj":"T19230","obj":"T19232"},{"id":"R14432","pred":"arg3Of","subj":"T19233","obj":"T19229"},{"id":"R14433","pred":"arg1Of","subj":"T19235","obj":"T19234"},{"id":"R14434","pred":"arg1Of","subj":"T19235","obj":"T19236"},{"id":"R14435","pred":"arg1Of","subj":"T19235","obj":"T19243"},{"id":"R14436","pred":"arg2Of","subj":"T19235","obj":"T19244"},{"id":"R14437","pred":"arg1Of","subj":"T19237","obj":"T19238"},{"id":"R14438","pred":"arg2Of","subj":"T19238","obj":"T19236"},{"id":"R14439","pred":"arg2Of","subj":"T19242","obj":"T19238"},{"id":"R14440","pred":"arg1Of","subj":"T19242","obj":"T19239"},{"id":"R14441","pred":"arg1Of","subj":"T19242","obj":"T19240"},{"id":"R14442","pred":"arg1Of","subj":"T19242","obj":"T19241"},{"id":"R14443","pred":"arg2Of","subj":"T19244","obj":"T19243"},{"id":"R14444","pred":"arg1Of","subj":"T19244","obj":"T19245"},{"id":"R14445","pred":"arg2Of","subj":"T19248","obj":"T19245"},{"id":"R14446","pred":"arg1Of","subj":"T19248","obj":"T19246"},{"id":"R14447","pred":"arg1Of","subj":"T19248","obj":"T19247"},{"id":"R14448","pred":"arg1Of","subj":"T19248","obj":"T19249"},{"id":"R14449","pred":"arg2Of","subj":"T19251","obj":"T19249"},{"id":"R14450","pred":"arg1Of","subj":"T19251","obj":"T19250"},{"id":"R14451","pred":"arg1Of","subj":"T19251","obj":"T19252"},{"id":"R14452","pred":"arg2Of","subj":"T19253","obj":"T19252"},{"id":"R14453","pred":"arg3Of","subj":"T19254","obj":"T19249"}],"namespaces":[{"prefix":"_base","uri":"http://kmcs.nii.ac.jp/enju/"}],"text":"Materials\nRecombinant human M-CSF was purchased from R\u0026D Systems (Minneapolis, MN). Ro-31-8220, Gö-6976, Rotterin, and BAPTA/AM were obtained from Calbiochem (San Diego, CA). Low endotoxin RPMI and X-vivo serum free medium were obtained from Lonza Walkersville Inc (Walkersville, MD). Fetal bovine serum (FBS, certified \u003c0.06 endotoxin units/ml endotoxin levels) was purchased from Atlanta Biological (Lawrenceville, GA). All other cell culture reagents were obtained through Invitrogen (Carlsbad, CA). PKCα NF-κB p65, Lamin B, β-actin, and GAPDH antibodies, anti-mouse and rabbit IgG-HRP conjugated antibodies, and PKCα siRNA were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Phospho-NFκB p65 (Ser276 or Ser536) antibodies were purchased from Cell Signaling Technology (Danvers, MA). DNA constructs pTAL-SEAP and pNF-κB-SEAP were obtained from Clontech (Mountian View, CA). Expression vectors containing CMV-p65 WT, CMV-p65 276S/A and CMV-p65 536S/A were cloned in pBa5e Puro vector or pFLAG-CMV-2 vector as described previously [49]. Kinase deficient CMV-PKCα (KD-PKCα) or wild type pCMV-PKCα (WT-PKCα) were generous gifts from Alexandra C. Newton (University of California, San Diego, CA). IκBα (NFKBIA) and GAPDH primers for real-time RT-PCR were obtained from SABiosciences (Frederick, MD). A pool of mouse or human PKCα specific siRNA were purchased from Santa Cruz Biotechnology (Santa cruz, CA)."}

{"project":"sentences","denotations":[{"id":"T18720","span":{"begin":0,"end":9},"obj":"Sentence"},{"id":"T18721","span":{"begin":10,"end":83},"obj":"Sentence"},{"id":"T18722","span":{"begin":84,"end":174},"obj":"Sentence"},{"id":"T18723","span":{"begin":175,"end":284},"obj":"Sentence"},{"id":"T18724","span":{"begin":285,"end":421},"obj":"Sentence"},{"id":"T18725","span":{"begin":422,"end":502},"obj":"Sentence"},{"id":"T18726","span":{"begin":503,"end":688},"obj":"Sentence"},{"id":"T18727","span":{"begin":689,"end":796},"obj":"Sentence"},{"id":"T18728","span":{"begin":797,"end":886},"obj":"Sentence"},{"id":"T18729","span":{"begin":887,"end":1047},"obj":"Sentence"},{"id":"T18730","span":{"begin":1048,"end":1204},"obj":"Sentence"},{"id":"T18731","span":{"begin":1205,"end":1307},"obj":"Sentence"},{"id":"T18732","span":{"begin":1308,"end":1415},"obj":"Sentence"},{"id":"T273","span":{"begin":0,"end":9},"obj":"Sentence"},{"id":"T274","span":{"begin":10,"end":83},"obj":"Sentence"},{"id":"T275","span":{"begin":84,"end":174},"obj":"Sentence"},{"id":"T276","span":{"begin":175,"end":284},"obj":"Sentence"},{"id":"T277","span":{"begin":285,"end":421},"obj":"Sentence"},{"id":"T278","span":{"begin":422,"end":502},"obj":"Sentence"},{"id":"T279","span":{"begin":503,"end":688},"obj":"Sentence"},{"id":"T280","span":{"begin":689,"end":796},"obj":"Sentence"},{"id":"T281","span":{"begin":797,"end":886},"obj":"Sentence"},{"id":"T282","span":{"begin":887,"end":1047},"obj":"Sentence"},{"id":"T283","span":{"begin":1048,"end":1154},"obj":"Sentence"},{"id":"T284","span":{"begin":1155,"end":1204},"obj":"Sentence"},{"id":"T285","span":{"begin":1205,"end":1307},"obj":"Sentence"},{"id":"T286","span":{"begin":1308,"end":1415},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Materials\nRecombinant human M-CSF was purchased from R\u0026D Systems (Minneapolis, MN). Ro-31-8220, Gö-6976, Rotterin, and BAPTA/AM were obtained from Calbiochem (San Diego, CA). Low endotoxin RPMI and X-vivo serum free medium were obtained from Lonza Walkersville Inc (Walkersville, MD). Fetal bovine serum (FBS, certified \u003c0.06 endotoxin units/ml endotoxin levels) was purchased from Atlanta Biological (Lawrenceville, GA). All other cell culture reagents were obtained through Invitrogen (Carlsbad, CA). PKCα NF-κB p65, Lamin B, β-actin, and GAPDH antibodies, anti-mouse and rabbit IgG-HRP conjugated antibodies, and PKCα siRNA were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Phospho-NFκB p65 (Ser276 or Ser536) antibodies were purchased from Cell Signaling Technology (Danvers, MA). DNA constructs pTAL-SEAP and pNF-κB-SEAP were obtained from Clontech (Mountian View, CA). Expression vectors containing CMV-p65 WT, CMV-p65 276S/A and CMV-p65 536S/A were cloned in pBa5e Puro vector or pFLAG-CMV-2 vector as described previously [49]. Kinase deficient CMV-PKCα (KD-PKCα) or wild type pCMV-PKCα (WT-PKCα) were generous gifts from Alexandra C. Newton (University of California, San Diego, CA). IκBα (NFKBIA) and GAPDH primers for real-time RT-PCR were obtained from SABiosciences (Frederick, MD). A pool of mouse or human PKCα specific siRNA were purchased from Santa Cruz Biotechnology (Santa cruz, CA)."}

{"project":"2_test","denotations":[{"id":"22216091-18299349-90609742","span":{"begin":1043,"end":1045},"obj":"18299349"}],"text":"Materials\nRecombinant human M-CSF was purchased from R\u0026D Systems (Minneapolis, MN). Ro-31-8220, Gö-6976, Rotterin, and BAPTA/AM were obtained from Calbiochem (San Diego, CA). Low endotoxin RPMI and X-vivo serum free medium were obtained from Lonza Walkersville Inc (Walkersville, MD). Fetal bovine serum (FBS, certified \u003c0.06 endotoxin units/ml endotoxin levels) was purchased from Atlanta Biological (Lawrenceville, GA). All other cell culture reagents were obtained through Invitrogen (Carlsbad, CA). PKCα NF-κB p65, Lamin B, β-actin, and GAPDH antibodies, anti-mouse and rabbit IgG-HRP conjugated antibodies, and PKCα siRNA were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Phospho-NFκB p65 (Ser276 or Ser536) antibodies were purchased from Cell Signaling Technology (Danvers, MA). DNA constructs pTAL-SEAP and pNF-κB-SEAP were obtained from Clontech (Mountian View, CA). Expression vectors containing CMV-p65 WT, CMV-p65 276S/A and CMV-p65 536S/A were cloned in pBa5e Puro vector or pFLAG-CMV-2 vector as described previously [49]. Kinase deficient CMV-PKCα (KD-PKCα) or wild type pCMV-PKCα (WT-PKCα) were generous gifts from Alexandra C. Newton (University of California, San Diego, CA). IκBα (NFKBIA) and GAPDH primers for real-time RT-PCR were obtained from SABiosciences (Frederick, MD). A pool of mouse or human PKCα specific siRNA were purchased from Santa Cruz Biotechnology (Santa cruz, CA)."}

{"project":"bionlp-st-ge-2016-spacy-parsed","denotations":[{"id":"T18772","span":{"begin":205,"end":210},"obj":"VBP"},{"id":"T18733","span":{"begin":0,"end":9},"obj":"NNPS"},{"id":"T18734","span":{"begin":10,"end":21},"obj":"NNP"},{"id":"T18735","span":{"begin":22,"end":27},"obj":"JJ"},{"id":"T18736","span":{"begin":28,"end":33},"obj":"NNP"},{"id":"T18737","span":{"begin":34,"end":37},"obj":"VBD"},{"id":"T18738","span":{"begin":38,"end":47},"obj":"VBN"},{"id":"T18739","span":{"begin":48,"end":52},"obj":"IN"},{"id":"T18740","span":{"begin":53,"end":56},"obj":"NNP"},{"id":"T18741","span":{"begin":57,"end":64},"obj":"NNP"},{"id":"T18742","span":{"begin":65,"end":66},"obj":"-LRB-"},{"id":"T18743","span":{"begin":66,"end":77},"obj":"NNP"},{"id":"T18744","span":{"begin":77,"end":78},"obj":","},{"id":"T18745","span":{"begin":79,"end":81},"obj":"NNP"},{"id":"T18746","span":{"begin":81,"end":82},"obj":"-RRB-"},{"id":"T18747","span":{"begin":82,"end":83},"obj":"."},{"id":"T18748","span":{"begin":84,"end":94},"obj":"NN"},{"id":"T18749","span":{"begin":94,"end":95},"obj":","},{"id":"T18750","span":{"begin":96,"end":103},"obj":"NNP"},{"id":"T18751","span":{"begin":103,"end":104},"obj":","},{"id":"T18752","span":{"begin":105,"end":113},"obj":"NNP"},{"id":"T18753","span":{"begin":113,"end":114},"obj":","},{"id":"T18754","span":{"begin":115,"end":118},"obj":"CC"},{"id":"T18755","span":{"begin":119,"end":127},"obj":"NNP"},{"id":"T18756","span":{"begin":128,"end":132},"obj":"VBD"},{"id":"T18757","span":{"begin":133,"end":141},"obj":"VBN"},{"id":"T18758","span":{"begin":142,"end":146},"obj":"IN"},{"id":"T18759","span":{"begin":147,"end":157},"obj":"NNP"},{"id":"T18760","span":{"begin":158,"end":159},"obj":"-LRB-"},{"id":"T18761","span":{"begin":159,"end":162},"obj":"NNP"},{"id":"T18762","span":{"begin":163,"end":168},"obj":"NNP"},{"id":"T18763","span":{"begin":168,"end":169},"obj":","},{"id":"T18764","span":{"begin":170,"end":172},"obj":"NNP"},{"id":"T18765","span":{"begin":172,"end":173},"obj":"-RRB-"},{"id":"T18766","span":{"begin":173,"end":174},"obj":"."},{"id":"T18767","span":{"begin":175,"end":178},"obj":"NNP"},{"id":"T18768","span":{"begin":179,"end":188},"obj":"NN"},{"id":"T18769","span":{"begin":189,"end":193},"obj":"NNP"},{"id":"T18770","span":{"begin":194,"end":197},"obj":"CC"},{"id":"T18771","span":{"begin":198,"end":204},"obj":"NNP"},{"id":"T18773","span":{"begin":211,"end":215},"obj":"JJ"},{"id":"T18774","span":{"begin":216,"end":222},"obj":"NN"},{"id":"T18775","span":{"begin":223,"end":227},"obj":"VBD"},{"id":"T18776","span":{"begin":228,"end":236},"obj":"VBN"},{"id":"T18777","span":{"begin":237,"end":241},"obj":"IN"},{"id":"T18778","span":{"begin":242,"end":247},"obj":"NNP"},{"id":"T18779","span":{"begin":248,"end":260},"obj":"NNP"},{"id":"T18780","span":{"begin":261,"end":264},"obj":"NNP"},{"id":"T18781","span":{"begin":265,"end":266},"obj":"-LRB-"},{"id":"T18782","span":{"begin":266,"end":278},"obj":"NNP"},{"id":"T18783","span":{"begin":278,"end":279},"obj":","},{"id":"T18784","span":{"begin":280,"end":282},"obj":"NNP"},{"id":"T18785","span":{"begin":282,"end":283},"obj":"-RRB-"},{"id":"T18786","span":{"begin":283,"end":284},"obj":"."},{"id":"T18787","span":{"begin":285,"end":290},"obj":"JJ"},{"id":"T18788","span":{"begin":291,"end":297},"obj":"JJ"},{"id":"T18789","span":{"begin":298,"end":303},"obj":"NN"},{"id":"T18790","span":{"begin":304,"end":305},"obj":"-LRB-"},{"id":"T18791","span":{"begin":305,"end":308},"obj":"NNP"},{"id":"T18792","span":{"begin":308,"end":309},"obj":","},{"id":"T18793","span":{"begin":310,"end":319},"obj":"VBD"},{"id":"T18794","span":{"begin":320,"end":321},"obj":"CD"},{"id":"T18795","span":{"begin":321,"end":325},"obj":"CD"},{"id":"T18796","span":{"begin":326,"end":335},"obj":"NN"},{"id":"T18797","span":{"begin":336,"end":344},"obj":"NN"},{"id":"T18798","span":{"begin":345,"end":354},"obj":"NN"},{"id":"T18799","span":{"begin":355,"end":361},"obj":"NNS"},{"id":"T18800","span":{"begin":361,"end":362},"obj":"-RRB-"},{"id":"T18801","span":{"begin":363,"end":366},"obj":"VBD"},{"id":"T18802","span":{"begin":367,"end":376},"obj":"VBN"},{"id":"T18803","span":{"begin":377,"end":381},"obj":"IN"},{"id":"T18804","span":{"begin":382,"end":389},"obj":"NNP"},{"id":"T18805","span":{"begin":390,"end":400},"obj":"NNP"},{"id":"T18806","span":{"begin":401,"end":402},"obj":"-LRB-"},{"id":"T18807","span":{"begin":402,"end":415},"obj":"NNP"},{"id":"T18808","span":{"begin":415,"end":416},"obj":","},{"id":"T18809","span":{"begin":417,"end":419},"obj":"NNP"},{"id":"T18810","span":{"begin":419,"end":420},"obj":"-RRB-"},{"id":"T18811","span":{"begin":420,"end":421},"obj":"."},{"id":"T18812","span":{"begin":422,"end":425},"obj":"DT"},{"id":"T18813","span":{"begin":426,"end":431},"obj":"JJ"},{"id":"T18814","span":{"begin":432,"end":436},"obj":"NN"},{"id":"T18815","span":{"begin":437,"end":444},"obj":"NN"},{"id":"T18816","span":{"begin":445,"end":453},"obj":"NNS"},{"id":"T18817","span":{"begin":454,"end":458},"obj":"VBD"},{"id":"T18818","span":{"begin":459,"end":467},"obj":"VBN"},{"id":"T18819","span":{"begin":468,"end":475},"obj":"IN"},{"id":"T18820","span":{"begin":476,"end":486},"obj":"NNP"},{"id":"T18821","span":{"begin":487,"end":488},"obj":"-LRB-"},{"id":"T18822","span":{"begin":488,"end":496},"obj":"NNP"},{"id":"T18823","span":{"begin":496,"end":497},"obj":","},{"id":"T18824","span":{"begin":498,"end":500},"obj":"NNP"},{"id":"T18825","span":{"begin":500,"end":501},"obj":"-RRB-"},{"id":"T18826","span":{"begin":501,"end":502},"obj":"."},{"id":"T18827","span":{"begin":503,"end":507},"obj":"NNP"},{"id":"T18828","span":{"begin":508,"end":513},"obj":"NNP"},{"id":"T18829","span":{"begin":514,"end":517},"obj":"NNS"},{"id":"T18830","span":{"begin":517,"end":518},"obj":","},{"id":"T18831","span":{"begin":519,"end":524},"obj":"NNP"},{"id":"T18832","span":{"begin":525,"end":526},"obj":"NNP"},{"id":"T18833","span":{"begin":526,"end":527},"obj":","},{"id":"T18834","span":{"begin":528,"end":535},"obj":"NN"},{"id":"T18835","span":{"begin":535,"end":536},"obj":","},{"id":"T18836","span":{"begin":537,"end":540},"obj":"CC"},{"id":"T18837","span":{"begin":541,"end":546},"obj":"NNP"},{"id":"T18838","span":{"begin":547,"end":557},"obj":"NNS"},{"id":"T18839","span":{"begin":557,"end":558},"obj":","},{"id":"T18840","span":{"begin":559,"end":569},"obj":"JJ"},{"id":"T18841","span":{"begin":570,"end":573},"obj":"CC"},{"id":"T18842","span":{"begin":574,"end":580},"obj":"NN"},{"id":"T18843","span":{"begin":581,"end":588},"obj":"JJ"},{"id":"T18844","span":{"begin":589,"end":599},"obj":"VBN"},{"id":"T18845","span":{"begin":600,"end":610},"obj":"NNS"},{"id":"T18846","span":{"begin":610,"end":611},"obj":","},{"id":"T18847","span":{"begin":612,"end":615},"obj":"CC"},{"id":"T18848","span":{"begin":616,"end":620},"obj":"NNP"},{"id":"T18849","span":{"begin":621,"end":626},"obj":"NNP"},{"id":"T18850","span":{"begin":627,"end":631},"obj":"VBD"},{"id":"T18851","span":{"begin":632,"end":640},"obj":"VBN"},{"id":"T18852","span":{"begin":641,"end":645},"obj":"IN"},{"id":"T18853","span":{"begin":646,"end":651},"obj":"NNP"},{"id":"T18854","span":{"begin":652,"end":656},"obj":"NNP"},{"id":"T18855","span":{"begin":657,"end":670},"obj":"NNP"},{"id":"T18856","span":{"begin":671,"end":672},"obj":"-LRB-"},{"id":"T18857","span":{"begin":672,"end":677},"obj":"NNP"},{"id":"T18858","span":{"begin":678,"end":682},"obj":"NNP"},{"id":"T18859","span":{"begin":682,"end":683},"obj":","},{"id":"T18860","span":{"begin":684,"end":686},"obj":"NNP"},{"id":"T18861","span":{"begin":686,"end":687},"obj":"-RRB-"},{"id":"T18862","span":{"begin":687,"end":688},"obj":"."},{"id":"T18863","span":{"begin":689,"end":701},"obj":"NNP"},{"id":"T18864","span":{"begin":702,"end":705},"obj":"NNS"},{"id":"T18865","span":{"begin":706,"end":707},"obj":"-LRB-"},{"id":"T18866","span":{"begin":707,"end":713},"obj":"CD"},{"id":"T18867","span":{"begin":714,"end":716},"obj":"CC"},{"id":"T18868","span":{"begin":717,"end":723},"obj":"CD"},{"id":"T18869","span":{"begin":723,"end":724},"obj":"-RRB-"},{"id":"T18870","span":{"begin":725,"end":735},"obj":"NNS"},{"id":"T18871","span":{"begin":736,"end":740},"obj":"VBD"},{"id":"T18872","span":{"begin":741,"end":750},"obj":"VBN"},{"id":"T18873","span":{"begin":751,"end":755},"obj":"IN"},{"id":"T18874","span":{"begin":756,"end":760},"obj":"NNP"},{"id":"T18875","span":{"begin":761,"end":770},"obj":"NNP"},{"id":"T18876","span":{"begin":771,"end":781},"obj":"NNP"},{"id":"T18877","span":{"begin":782,"end":783},"obj":"-LRB-"},{"id":"T18878","span":{"begin":783,"end":790},"obj":"NNP"},{"id":"T18879","span":{"begin":790,"end":791},"obj":","},{"id":"T18880","span":{"begin":792,"end":794},"obj":"NNP"},{"id":"T18881","span":{"begin":794,"end":795},"obj":"-RRB-"},{"id":"T18882","span":{"begin":795,"end":796},"obj":"."},{"id":"T18883","span":{"begin":797,"end":800},"obj":"NNP"},{"id":"T18884","span":{"begin":801,"end":811},"obj":"VBZ"},{"id":"T18885","span":{"begin":812,"end":821},"obj":"NNP"},{"id":"T18886","span":{"begin":822,"end":825},"obj":"CC"},{"id":"T18887","span":{"begin":826,"end":837},"obj":"NNP"},{"id":"T18888","span":{"begin":838,"end":842},"obj":"VBD"},{"id":"T18889","span":{"begin":843,"end":851},"obj":"VBN"},{"id":"T18890","span":{"begin":852,"end":856},"obj":"IN"},{"id":"T18891","span":{"begin":857,"end":865},"obj":"NNP"},{"id":"T18892","span":{"begin":866,"end":867},"obj":"-LRB-"},{"id":"T18893","span":{"begin":867,"end":875},"obj":"NNP"},{"id":"T18894","span":{"begin":876,"end":880},"obj":"NNP"},{"id":"T18895","span":{"begin":880,"end":881},"obj":","},{"id":"T18896","span":{"begin":882,"end":884},"obj":"NNP"},{"id":"T18897","span":{"begin":884,"end":885},"obj":"-RRB-"},{"id":"T18898","span":{"begin":885,"end":886},"obj":"."},{"id":"T18899","span":{"begin":887,"end":897},"obj":"NN"},{"id":"T18900","span":{"begin":898,"end":905},"obj":"NNS"},{"id":"T18901","span":{"begin":906,"end":916},"obj":"VBG"},{"id":"T18902","span":{"begin":917,"end":924},"obj":"NNP"},{"id":"T18903","span":{"begin":925,"end":927},"obj":"NNP"},{"id":"T18904","span":{"begin":927,"end":928},"obj":","},{"id":"T18905","span":{"begin":929,"end":936},"obj":"NNP"},{"id":"T18906","span":{"begin":937,"end":943},"obj":"NNP"},{"id":"T18907","span":{"begin":944,"end":947},"obj":"CC"},{"id":"T18908","span":{"begin":948,"end":955},"obj":"NNP"},{"id":"T18909","span":{"begin":956,"end":962},"obj":"NNP"},{"id":"T18910","span":{"begin":963,"end":967},"obj":"VBD"},{"id":"T18911","span":{"begin":968,"end":974},"obj":"VBN"},{"id":"T18912","span":{"begin":975,"end":977},"obj":"IN"},{"id":"T18913","span":{"begin":978,"end":983},"obj":"NNP"},{"id":"T18914","span":{"begin":984,"end":988},"obj":"NNP"},{"id":"T18915","span":{"begin":989,"end":995},"obj":"NN"},{"id":"T18916","span":{"begin":996,"end":998},"obj":"CC"},{"id":"T18917","span":{"begin":999,"end":1010},"obj":"NN"},{"id":"T18918","span":{"begin":1011,"end":1017},"obj":"NN"},{"id":"T18919","span":{"begin":1018,"end":1020},"obj":"IN"},{"id":"T18920","span":{"begin":1021,"end":1030},"obj":"VBN"},{"id":"T18921","span":{"begin":1031,"end":1041},"obj":"RB"},{"id":"T18922","span":{"begin":1042,"end":1043},"obj":"NNP"},{"id":"T18923","span":{"begin":1043,"end":1045},"obj":"CD"},{"id":"T18924","span":{"begin":1045,"end":1046},"obj":"NNP"},{"id":"T18925","span":{"begin":1046,"end":1047},"obj":"."},{"id":"T18926","span":{"begin":1048,"end":1054},"obj":"NNP"},{"id":"T18927","span":{"begin":1055,"end":1064},"obj":"NN"},{"id":"T18928","span":{"begin":1065,"end":1073},"obj":"NNP"},{"id":"T18929","span":{"begin":1074,"end":1075},"obj":"-LRB-"},{"id":"T18930","span":{"begin":1075,"end":1082},"obj":"NNP"},{"id":"T18931","span":{"begin":1082,"end":1083},"obj":"-RRB-"},{"id":"T18932","span":{"begin":1084,"end":1086},"obj":"CC"},{"id":"T18933","span":{"begin":1087,"end":1091},"obj":"JJ"},{"id":"T18934","span":{"begin":1092,"end":1096},"obj":"NN"},{"id":"T18935","span":{"begin":1097,"end":1106},"obj":"NN"},{"id":"T18936","span":{"begin":1107,"end":1108},"obj":"-LRB-"},{"id":"T18937","span":{"begin":1108,"end":1115},"obj":"NNP"},{"id":"T18938","span":{"begin":1115,"end":1116},"obj":"-RRB-"},{"id":"T18939","span":{"begin":1117,"end":1121},"obj":"VBD"},{"id":"T18940","span":{"begin":1122,"end":1130},"obj":"JJ"},{"id":"T18941","span":{"begin":1131,"end":1136},"obj":"NNS"},{"id":"T18942","span":{"begin":1137,"end":1141},"obj":"IN"},{"id":"T18943","span":{"begin":1142,"end":1151},"obj":"NNP"},{"id":"T18944","span":{"begin":1152,"end":1154},"obj":"NNP"},{"id":"T18945","span":{"begin":1155,"end":1161},"obj":"NNP"},{"id":"T18946","span":{"begin":1162,"end":1163},"obj":"-LRB-"},{"id":"T18947","span":{"begin":1163,"end":1173},"obj":"NNP"},{"id":"T18948","span":{"begin":1174,"end":1176},"obj":"IN"},{"id":"T18949","span":{"begin":1177,"end":1187},"obj":"NNP"},{"id":"T18950","span":{"begin":1187,"end":1188},"obj":","},{"id":"T18951","span":{"begin":1189,"end":1192},"obj":"NNP"},{"id":"T18952","span":{"begin":1193,"end":1198},"obj":"NNP"},{"id":"T18953","span":{"begin":1198,"end":1199},"obj":","},{"id":"T18954","span":{"begin":1200,"end":1202},"obj":"NNP"},{"id":"T18955","span":{"begin":1202,"end":1203},"obj":"-RRB-"},{"id":"T18956","span":{"begin":1203,"end":1204},"obj":"."},{"id":"T18957","span":{"begin":1205,"end":1209},"obj":"NNP"},{"id":"T18958","span":{"begin":1210,"end":1211},"obj":"-LRB-"},{"id":"T18959","span":{"begin":1211,"end":1217},"obj":"NNP"},{"id":"T18960","span":{"begin":1217,"end":1218},"obj":"-RRB-"},{"id":"T18961","span":{"begin":1219,"end":1222},"obj":"CC"},{"id":"T18962","span":{"begin":1223,"end":1228},"obj":"NNP"},{"id":"T18963","span":{"begin":1229,"end":1236},"obj":"NNS"},{"id":"T18964","span":{"begin":1237,"end":1240},"obj":"IN"},{"id":"T18965","span":{"begin":1241,"end":1250},"obj":"JJ"},{"id":"T18966","span":{"begin":1251,"end":1257},"obj":"NN"},{"id":"T18967","span":{"begin":1258,"end":1262},"obj":"VBD"},{"id":"T18968","span":{"begin":1263,"end":1271},"obj":"VBN"},{"id":"T18969","span":{"begin":1272,"end":1276},"obj":"IN"},{"id":"T18970","span":{"begin":1277,"end":1290},"obj":"NNS"},{"id":"T18971","span":{"begin":1291,"end":1292},"obj":"-LRB-"},{"id":"T18972","span":{"begin":1292,"end":1301},"obj":"NNP"},{"id":"T18973","span":{"begin":1301,"end":1302},"obj":","},{"id":"T18974","span":{"begin":1303,"end":1305},"obj":"NNP"},{"id":"T18975","span":{"begin":1305,"end":1306},"obj":"-RRB-"},{"id":"T18976","span":{"begin":1306,"end":1307},"obj":"."},{"id":"T18977","span":{"begin":1308,"end":1309},"obj":"DT"},{"id":"T18978","span":{"begin":1310,"end":1314},"obj":"NN"},{"id":"T18979","span":{"begin":1315,"end":1317},"obj":"IN"},{"id":"T18980","span":{"begin":1318,"end":1323},"obj":"NN"},{"id":"T18981","span":{"begin":1324,"end":1326},"obj":"CC"},{"id":"T18982","span":{"begin":1327,"end":1332},"obj":"JJ"},{"id":"T18983","span":{"begin":1333,"end":1337},"obj":"NNP"},{"id":"T18984","span":{"begin":1338,"end":1346},"obj":"JJ"},{"id":"T18985","span":{"begin":1347,"end":1352},"obj":"NNP"},{"id":"T18986","span":{"begin":1353,"end":1357},"obj":"VBD"},{"id":"T18987","span":{"begin":1358,"end":1367},"obj":"VBN"},{"id":"T18988","span":{"begin":1368,"end":1372},"obj":"IN"},{"id":"T18989","span":{"begin":1373,"end":1378},"obj":"NNP"},{"id":"T18990","span":{"begin":1379,"end":1383},"obj":"NNP"},{"id":"T18991","span":{"begin":1384,"end":1397},"obj":"NNP"},{"id":"T18992","span":{"begin":1398,"end":1399},"obj":"-LRB-"},{"id":"T18993","span":{"begin":1399,"end":1404},"obj":"NNP"},{"id":"T18994","span":{"begin":1405,"end":1409},"obj":"NNP"},{"id":"T18995","span":{"begin":1409,"end":1410},"obj":","},{"id":"T18996","span":{"begin":1411,"end":1413},"obj":"NNP"},{"id":"T18997","span":{"begin":1413,"end":1414},"obj":"-RRB-"},{"id":"T18998","span":{"begin":1414,"end":1415},"obj":"."}],"relations":[{"id":"R13934","pred":"compound","subj":"T18733","obj":"T18736"},{"id":"R13935","pred":"compound","subj":"T18734","obj":"T18736"},{"id":"R13936","pred":"compound","subj":"T18735","obj":"T18736"},{"id":"R13937","pred":"nsubjpass","subj":"T18736","obj":"T18738"},{"id":"R13938","pred":"auxpass","subj":"T18737","obj":"T18738"},{"id":"R13939","pred":"ROOT","subj":"T18738","obj":"T18738"},{"id":"R13940","pred":"prep","subj":"T18739","obj":"T18738"},{"id":"R13941","pred":"compound","subj":"T18740","obj":"T18741"},{"id":"R13942","pred":"pobj","subj":"T18741","obj":"T18739"},{"id":"R13943","pred":"punct","subj":"T18742","obj":"T18741"},{"id":"R13944","pred":"appos","subj":"T18743","obj":"T18741"},{"id":"R13945","pred":"punct","subj":"T18744","obj":"T18743"},{"id":"R13946","pred":"appos","subj":"T18745","obj":"T18743"},{"id":"R13947","pred":"punct","subj":"T18746","obj":"T18743"},{"id":"R13948","pred":"punct","subj":"T18747","obj":"T18738"},{"id":"R13949","pred":"nsubjpass","subj":"T18748","obj":"T18757"},{"id":"R13950","pred":"punct","subj":"T18749","obj":"T18748"},{"id":"R13951","pred":"conj","subj":"T18750","obj":"T18748"},{"id":"R13952","pred":"punct","subj":"T18751","obj":"T18750"},{"id":"R13953","pred":"conj","subj":"T18752","obj":"T18750"},{"id":"R13954","pred":"punct","subj":"T18753","obj":"T18752"},{"id":"R13955","pred":"cc","subj":"T18754","obj":"T18752"},{"id":"R13956","pred":"conj","subj":"T18755","obj":"T18752"},{"id":"R13957","pred":"auxpass","subj":"T18756","obj":"T18757"},{"id":"R13958","pred":"ROOT","subj":"T18757","obj":"T18757"},{"id":"R13959","pred":"prep","subj":"T18758","obj":"T18757"},{"id":"R13960","pred":"pobj","subj":"T18759","obj":"T18758"},{"id":"R13961","pred":"punct","subj":"T18760","obj":"T18759"},{"id":"R13962","pred":"compound","subj":"T18761","obj":"T18762"},{"id":"R13963","pred":"appos","subj":"T18762","obj":"T18759"},{"id":"R13964","pred":"punct","subj":"T18763","obj":"T18762"},{"id":"R13965","pred":"conj","subj":"T18764","obj":"T18759"},{"id":"R13966","pred":"punct","subj":"T18765","obj":"T18757"},{"id":"R13967","pred":"punct","subj":"T18766","obj":"T18757"},{"id":"R13968","pred":"amod","subj":"T18767","obj":"T18769"},{"id":"R13969","pred":"compound","subj":"T18768","obj":"T18769"},{"id":"R13970","pred":"nsubj","subj":"T18769","obj":"T18772"},{"id":"R13971","pred":"cc","subj":"T18770","obj":"T18769"},{"id":"R13972","pred":"conj","subj":"T18771","obj":"T18769"},{"id":"R13973","pred":"ROOT","subj":"T18772","obj":"T18772"},{"id":"R13974","pred":"amod","subj":"T18773","obj":"T18774"},{"id":"R13975","pred":"nsubjpass","subj":"T18774","obj":"T18776"},{"id":"R13976","pred":"auxpass","subj":"T18775","obj":"T18776"},{"id":"R13977","pred":"ccomp","subj":"T18776","obj":"T18772"},{"id":"R13978","pred":"prep","subj":"T18777","obj":"T18776"},{"id":"R13979","pred":"compound","subj":"T18778","obj":"T18780"},{"id":"R13980","pred":"compound","subj":"T18779","obj":"T18780"},{"id":"R13981","pred":"pobj","subj":"T18780","obj":"T18777"},{"id":"R13982","pred":"punct","subj":"T18781","obj":"T18780"},{"id":"R13983","pred":"appos","subj":"T18782","obj":"T18780"},{"id":"R13984","pred":"punct","subj":"T18783","obj":"T18782"},{"id":"R13985","pred":"appos","subj":"T18784","obj":"T18782"},{"id":"R13986","pred":"punct","subj":"T18785","obj":"T18782"},{"id":"R13987","pred":"punct","subj":"T18786","obj":"T18772"},{"id":"R13988","pred":"amod","subj":"T18787","obj":"T18789"},{"id":"R13989","pred":"amod","subj":"T18788","obj":"T18789"},{"id":"R13990","pred":"nsubj","subj":"T18789","obj":"T18793"},{"id":"R13991","pred":"punct","subj":"T18790","obj":"T18791"},{"id":"R13992","pred":"appos","subj":"T18791","obj":"T18789"},{"id":"R13993","pred":"punct","subj":"T18792","obj":"T18789"},{"id":"R13994","pred":"ROOT","subj":"T18793","obj":"T18793"},{"id":"R13995","pred":"compound","subj":"T18794","obj":"T18795"},{"id":"R13996","pred":"nummod","subj":"T18795","obj":"T18799"},{"id":"R13997","pred":"nmod","subj":"T18796","obj":"T18799"},{"id":"R13998","pred":"nmod","subj":"T18797","obj":"T18799"},{"id":"R13999","pred":"compound","subj":"T18798","obj":"T18799"},{"id":"R14000","pred":"dobj","subj":"T18799","obj":"T18793"},{"id":"R14001","pred":"punct","subj":"T18800","obj":"T18799"},{"id":"R14002","pred":"auxpass","subj":"T18801","obj":"T18802"},{"id":"R14003","pred":"conj","subj":"T18802","obj":"T18793"},{"id":"R14004","pred":"prep","subj":"T18803","obj":"T18802"},{"id":"R14005","pred":"compound","subj":"T18804","obj":"T18805"},{"id":"R14006","pred":"pobj","subj":"T18805","obj":"T18803"},{"id":"R14007","pred":"punct","subj":"T18806","obj":"T18805"},{"id":"R14008","pred":"appos","subj":"T18807","obj":"T18805"},{"id":"R14009","pred":"punct","subj":"T18808","obj":"T18807"},{"id":"R14010","pred":"conj","subj":"T18809","obj":"T18807"},{"id":"R14011","pred":"punct","subj":"T18810","obj":"T18805"},{"id":"R14012","pred":"punct","subj":"T18811","obj":"T18793"},{"id":"R14013","pred":"det","subj":"T18812","obj":"T18816"},{"id":"R14014","pred":"amod","subj":"T18813","obj":"T18816"},{"id":"R14015","pred":"compound","subj":"T18814","obj":"T18815"},{"id":"R14016","pred":"compound","subj":"T18815","obj":"T18816"},{"id":"R14017","pred":"nsubjpass","subj":"T18816","obj":"T18818"},{"id":"R14018","pred":"auxpass","subj":"T18817","obj":"T18818"},{"id":"R14019","pred":"ROOT","subj":"T18818","obj":"T18818"},{"id":"R14020","pred":"prep","subj":"T18819","obj":"T18818"},{"id":"R14021","pred":"pobj","subj":"T18820","obj":"T18819"},{"id":"R14022","pred":"punct","subj":"T18821","obj":"T18820"},{"id":"R14023","pred":"appos","subj":"T18822","obj":"T18820"},{"id":"R14024","pred":"punct","subj":"T18823","obj":"T18822"},{"id":"R14025","pred":"conj","subj":"T18824","obj":"T18820"},{"id":"R14026","pred":"punct","subj":"T18825","obj":"T18820"},{"id":"R14027","pred":"punct","subj":"T18826","obj":"T18818"},{"id":"R14028","pred":"compound","subj":"T18827","obj":"T18829"},{"id":"R14029","pred":"compound","subj":"T18828","obj":"T18829"},{"id":"R14030","pred":"nsubjpass","subj":"T18829","obj":"T18851"},{"id":"R14031","pred":"punct","subj":"T18830","obj":"T18829"},{"id":"R14032","pred":"compound","subj":"T18831","obj":"T18832"},{"id":"R14033","pred":"appos","subj":"T18832","obj":"T18829"},{"id":"R14034","pred":"punct","subj":"T18833","obj":"T18832"},{"id":"R14035","pred":"appos","subj":"T18834","obj":"T18832"},{"id":"R14036","pred":"punct","subj":"T18835","obj":"T18832"},{"id":"R14037","pred":"cc","subj":"T18836","obj":"T18832"},{"id":"R14038","pred":"conj","subj":"T18837","obj":"T18832"},{"id":"R14039","pred":"appos","subj":"T18838","obj":"T18832"},{"id":"R14040","pred":"punct","subj":"T18839","obj":"T18838"},{"id":"R14041","pred":"conj","subj":"T18840","obj":"T18838"},{"id":"R14042","pred":"cc","subj":"T18841","obj":"T18840"},{"id":"R14043","pred":"conj","subj":"T18842","obj":"T18840"},{"id":"R14044","pred":"amod","subj":"T18843","obj":"T18845"},{"id":"R14045","pred":"amod","subj":"T18844","obj":"T18845"},{"id":"R14046","pred":"conj","subj":"T18845","obj":"T18840"},{"id":"R14047","pred":"punct","subj":"T18846","obj":"T18845"},{"id":"R14048","pred":"cc","subj":"T18847","obj":"T18840"},{"id":"R14049","pred":"compound","subj":"T18848","obj":"T18849"},{"id":"R14050","pred":"appos","subj":"T18849","obj":"T18832"},{"id":"R14051","pred":"auxpass","subj":"T18850","obj":"T18851"},{"id":"R14052","pred":"ROOT","subj":"T18851","obj":"T18851"},{"id":"R14053","pred":"prep","subj":"T18852","obj":"T18851"},{"id":"R14054","pred":"compound","subj":"T18853","obj":"T18855"},{"id":"R14055","pred":"compound","subj":"T18854","obj":"T18855"},{"id":"R14056","pred":"pobj","subj":"T18855","obj":"T18852"},{"id":"R14057","pred":"punct","subj":"T18856","obj":"T18855"},{"id":"R14058","pred":"compound","subj":"T18857","obj":"T18858"},{"id":"R14059","pred":"appos","subj":"T18858","obj":"T18855"},{"id":"R14060","pred":"punct","subj":"T18859","obj":"T18855"},{"id":"R14061","pred":"appos","subj":"T18860","obj":"T18855"},{"id":"R14062","pred":"punct","subj":"T18861","obj":"T18855"},{"id":"R14063","pred":"punct","subj":"T18862","obj":"T18851"},{"id":"R14064","pred":"compound","subj":"T18863","obj":"T18864"},{"id":"R14065","pred":"nsubjpass","subj":"T18864","obj":"T18872"},{"id":"R14066","pred":"punct","subj":"T18865","obj":"T18864"},{"id":"R14067","pred":"appos","subj":"T18866","obj":"T18864"},{"id":"R14068","pred":"cc","subj":"T18867","obj":"T18866"},{"id":"R14069","pred":"conj","subj":"T18868","obj":"T18866"},{"id":"R14070","pred":"punct","subj":"T18869","obj":"T18864"},{"id":"R14071","pred":"conj","subj":"T18870","obj":"T18864"},{"id":"R14072","pred":"auxpass","subj":"T18871","obj":"T18872"},{"id":"R14073","pred":"ROOT","subj":"T18872","obj":"T18872"},{"id":"R14074","pred":"prep","subj":"T18873","obj":"T18872"},{"id":"R14075","pred":"compound","subj":"T18874","obj":"T18876"},{"id":"R14076","pred":"compound","subj":"T18875","obj":"T18876"},{"id":"R14077","pred":"pobj","subj":"T18876","obj":"T18873"},{"id":"R14078","pred":"punct","subj":"T18877","obj":"T18880"},{"id":"R14079","pred":"npadvmod","subj":"T18878","obj":"T18880"},{"id":"R14080","pred":"punct","subj":"T18879","obj":"T18880"},{"id":"R14081","pred":"appos","subj":"T18880","obj":"T18876"},{"id":"R14082","pred":"punct","subj":"T18881","obj":"T18880"},{"id":"R14083","pred":"punct","subj":"T18882","obj":"T18872"},{"id":"R14084","pred":"nsubj","subj":"T18883","obj":"T18884"},{"id":"R14085","pred":"ROOT","subj":"T18884","obj":"T18884"},{"id":"R14086","pred":"dobj","subj":"T18885","obj":"T18884"},{"id":"R14087","pred":"cc","subj":"T18886","obj":"T18885"},{"id":"R14088","pred":"conj","subj":"T18887","obj":"T18885"},{"id":"R14089","pred":"auxpass","subj":"T18888","obj":"T18889"},{"id":"R14090","pred":"conj","subj":"T18889","obj":"T18884"},{"id":"R14091","pred":"prep","subj":"T18890","obj":"T18889"},{"id":"R14092","pred":"pobj","subj":"T18891","obj":"T18890"},{"id":"R14093","pred":"punct","subj":"T18892","obj":"T18891"},{"id":"R14094","pred":"compound","subj":"T18893","obj":"T18894"},{"id":"R14095","pred":"appos","subj":"T18894","obj":"T18891"},{"id":"R14096","pred":"punct","subj":"T18895","obj":"T18894"},{"id":"R14097","pred":"npadvmod","subj":"T18896","obj":"T18894"},{"id":"R14098","pred":"punct","subj":"T18897","obj":"T18891"},{"id":"R14099","pred":"punct","subj":"T18898","obj":"T18884"},{"id":"R14100","pred":"compound","subj":"T18899","obj":"T18900"},{"id":"R14101","pred":"nsubjpass","subj":"T18900","obj":"T18911"},{"id":"R14102","pred":"acl","subj":"T18901","obj":"T18900"},{"id":"R14103","pred":"compound","subj":"T18902","obj":"T18903"},{"id":"R14104","pred":"dobj","subj":"T18903","obj":"T18901"},{"id":"R14105","pred":"punct","subj":"T18904","obj":"T18903"},{"id":"R14106","pred":"compound","subj":"T18905","obj":"T18906"},{"id":"R14107","pred":"conj","subj":"T18906","obj":"T18903"},{"id":"R14108","pred":"cc","subj":"T18907","obj":"T18906"},{"id":"R14109","pred":"compound","subj":"T18908","obj":"T18909"},{"id":"R14110","pred":"conj","subj":"T18909","obj":"T18906"},{"id":"R14111","pred":"auxpass","subj":"T18910","obj":"T18911"},{"id":"R14112","pred":"ROOT","subj":"T18911","obj":"T18911"},{"id":"R14113","pred":"prep","subj":"T18912","obj":"T18911"},{"id":"R14114","pred":"compound","subj":"T18913","obj":"T18915"},{"id":"R14115","pred":"compound","subj":"T18914","obj":"T18915"},{"id":"R14116","pred":"pobj","subj":"T18915","obj":"T18912"},{"id":"R14117","pred":"cc","subj":"T18916","obj":"T18915"},{"id":"R14118","pred":"compound","subj":"T18917","obj":"T18918"},{"id":"R14119","pred":"conj","subj":"T18918","obj":"T18915"},{"id":"R14120","pred":"mark","subj":"T18919","obj":"T18920"},{"id":"R14121","pred":"advcl","subj":"T18920","obj":"T18911"},{"id":"R14122","pred":"advmod","subj":"T18921","obj":"T18924"},{"id":"R14123","pred":"nmod","subj":"T18922","obj":"T18924"},{"id":"R14124","pred":"nummod","subj":"T18923","obj":"T18924"},{"id":"R14125","pred":"dobj","subj":"T18924","obj":"T18920"},{"id":"R14126","pred":"punct","subj":"T18925","obj":"T18911"},{"id":"R14127","pred":"nsubj","subj":"T18926","obj":"T18939"},{"id":"R14128","pred":"advmod","subj":"T18927","obj":"T18939"},{"id":"R14129","pred":"nmod","subj":"T18928","obj":"T18935"},{"id":"R14130","pred":"punct","subj":"T18929","obj":"T18930"},{"id":"R14131","pred":"appos","subj":"T18930","obj":"T18928"},{"id":"R14132","pred":"punct","subj":"T18931","obj":"T18930"},{"id":"R14133","pred":"cc","subj":"T18932","obj":"T18928"},{"id":"R14134","pred":"amod","subj":"T18933","obj":"T18934"},{"id":"R14135","pred":"compound","subj":"T18934","obj":"T18935"},{"id":"R14136","pred":"nsubj","subj":"T18935","obj":"T18939"},{"id":"R14137","pred":"punct","subj":"T18936","obj":"T18935"},{"id":"R14138","pred":"appos","subj":"T18937","obj":"T18935"},{"id":"R14139","pred":"punct","subj":"T18938","obj":"T18935"},{"id":"R14140","pred":"ROOT","subj":"T18939","obj":"T18939"},{"id":"R14141","pred":"amod","subj":"T18940","obj":"T18941"},{"id":"R14142","pred":"attr","subj":"T18941","obj":"T18939"},{"id":"R14143","pred":"prep","subj":"T18942","obj":"T18941"},{"id":"R14144","pred":"compound","subj":"T18943","obj":"T18945"},{"id":"R14145","pred":"compound","subj":"T18944","obj":"T18945"},{"id":"R14146","pred":"pobj","subj":"T18945","obj":"T18942"},{"id":"R14147","pred":"punct","subj":"T18946","obj":"T18945"},{"id":"R14148","pred":"appos","subj":"T18947","obj":"T18945"},{"id":"R14149","pred":"prep","subj":"T18948","obj":"T18947"},{"id":"R14150","pred":"pobj","subj":"T18949","obj":"T18948"},{"id":"R14151","pred":"punct","subj":"T18950","obj":"T18945"},{"id":"R14152","pred":"compound","subj":"T18951","obj":"T18952"},{"id":"R14153","pred":"npadvmod","subj":"T18952","obj":"T18945"},{"id":"R14154","pred":"punct","subj":"T18953","obj":"T18952"},{"id":"R14155","pred":"appos","subj":"T18954","obj":"T18952"},{"id":"R14156","pred":"punct","subj":"T18955","obj":"T18945"},{"id":"R14157","pred":"punct","subj":"T18956","obj":"T18939"},{"id":"R14158","pred":"nsubjpass","subj":"T18957","obj":"T18968"},{"id":"R14159","pred":"punct","subj":"T18958","obj":"T18959"},{"id":"R14160","pred":"appos","subj":"T18959","obj":"T18957"},{"id":"R14161","pred":"punct","subj":"T18960","obj":"T18959"},{"id":"R14162","pred":"cc","subj":"T18961","obj":"T18957"},{"id":"R14163","pred":"conj","subj":"T18962","obj":"T18957"},{"id":"R14164","pred":"nsubjpass","subj":"T18963","obj":"T18968"},{"id":"R14165","pred":"prep","subj":"T18964","obj":"T18963"},{"id":"R14166","pred":"amod","subj":"T18965","obj":"T18966"},{"id":"R14167","pred":"pobj","subj":"T18966","obj":"T18964"},{"id":"R14168","pred":"auxpass","subj":"T18967","obj":"T18968"},{"id":"R14169","pred":"ROOT","subj":"T18968","obj":"T18968"},{"id":"R14170","pred":"prep","subj":"T18969","obj":"T18968"},{"id":"R14171","pred":"pobj","subj":"T18970","obj":"T18969"},{"id":"R14172","pred":"punct","subj":"T18971","obj":"T18970"},{"id":"R14173","pred":"appos","subj":"T18972","obj":"T18970"},{"id":"R14174","pred":"punct","subj":"T18973","obj":"T18972"},{"id":"R14175","pred":"conj","subj":"T18974","obj":"T18972"},{"id":"R14176","pred":"punct","subj":"T18975","obj":"T18972"},{"id":"R14177","pred":"punct","subj":"T18976","obj":"T18968"},{"id":"R14178","pred":"det","subj":"T18977","obj":"T18978"},{"id":"R14179","pred":"nsubjpass","subj":"T18978","obj":"T18987"},{"id":"R14180","pred":"prep","subj":"T18979","obj":"T18978"},{"id":"R14181","pred":"pobj","subj":"T18980","obj":"T18979"},{"id":"R14182","pred":"cc","subj":"T18981","obj":"T18980"},{"id":"R14183","pred":"amod","subj":"T18982","obj":"T18985"},{"id":"R14184","pred":"compound","subj":"T18983","obj":"T18985"},{"id":"R14185","pred":"amod","subj":"T18984","obj":"T18985"},{"id":"R14186","pred":"appos","subj":"T18985","obj":"T18978"},{"id":"R14187","pred":"auxpass","subj":"T18986","obj":"T18987"},{"id":"R14188","pred":"ROOT","subj":"T18987","obj":"T18987"},{"id":"R14189","pred":"prep","subj":"T18988","obj":"T18987"},{"id":"R14190","pred":"compound","subj":"T18989","obj":"T18991"},{"id":"R14191","pred":"compound","subj":"T18990","obj":"T18991"},{"id":"R14192","pred":"pobj","subj":"T18991","obj":"T18988"},{"id":"R14193","pred":"punct","subj":"T18992","obj":"T18991"},{"id":"R14194","pred":"compound","subj":"T18993","obj":"T18994"},{"id":"R14195","pred":"appos","subj":"T18994","obj":"T18991"},{"id":"R14196","pred":"punct","subj":"T18995","obj":"T18991"},{"id":"R14197","pred":"appos","subj":"T18996","obj":"T18991"},{"id":"R14198","pred":"punct","subj":"T18997","obj":"T18987"},{"id":"R14199","pred":"punct","subj":"T18998","obj":"T18987"}],"text":"Materials\nRecombinant human M-CSF was purchased from R\u0026D Systems (Minneapolis, MN). Ro-31-8220, Gö-6976, Rotterin, and BAPTA/AM were obtained from Calbiochem (San Diego, CA). Low endotoxin RPMI and X-vivo serum free medium were obtained from Lonza Walkersville Inc (Walkersville, MD). Fetal bovine serum (FBS, certified \u003c0.06 endotoxin units/ml endotoxin levels) was purchased from Atlanta Biological (Lawrenceville, GA). All other cell culture reagents were obtained through Invitrogen (Carlsbad, CA). PKCα NF-κB p65, Lamin B, β-actin, and GAPDH antibodies, anti-mouse and rabbit IgG-HRP conjugated antibodies, and PKCα siRNA were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Phospho-NFκB p65 (Ser276 or Ser536) antibodies were purchased from Cell Signaling Technology (Danvers, MA). DNA constructs pTAL-SEAP and pNF-κB-SEAP were obtained from Clontech (Mountian View, CA). Expression vectors containing CMV-p65 WT, CMV-p65 276S/A and CMV-p65 536S/A were cloned in pBa5e Puro vector or pFLAG-CMV-2 vector as described previously [49]. Kinase deficient CMV-PKCα (KD-PKCα) or wild type pCMV-PKCα (WT-PKCα) were generous gifts from Alexandra C. Newton (University of California, San Diego, CA). IκBα (NFKBIA) and GAPDH primers for real-time RT-PCR were obtained from SABiosciences (Frederick, MD). A pool of mouse or human PKCα specific siRNA were purchased from Santa Cruz Biotechnology (Santa cruz, CA)."}

{"project":"test2","denotations":[{"id":"T18702","span":{"begin":28,"end":33},"obj":"Protein"},{"id":"T18703","span":{"begin":616,"end":626},"obj":"Protein"},{"id":"T18704","span":{"begin":689,"end":696},"obj":"Phosphorylation"},{"id":"T18705","span":{"begin":717,"end":723},"obj":"Entity"},{"id":"T18706","span":{"begin":921,"end":924},"obj":"Protein"},{"id":"T18707","span":{"begin":933,"end":936},"obj":"Protein"},{"id":"T18708","span":{"begin":952,"end":955},"obj":"Protein"},{"id":"T18709","span":{"begin":1048,"end":1054},"obj":"Negative_regulation"},{"id":"T18710","span":{"begin":1055,"end":1064},"obj":"Negative_regulation"},{"id":"T18711","span":{"begin":1069,"end":1073},"obj":"Protein"},{"id":"T18712","span":{"begin":1075,"end":1077},"obj":"Negative_regulation"},{"id":"T18713","span":{"begin":1078,"end":1082},"obj":"Protein"},{"id":"T18714","span":{"begin":1102,"end":1106},"obj":"Protein"},{"id":"T18715","span":{"begin":1111,"end":1115},"obj":"Protein"},{"id":"T18716","span":{"begin":1205,"end":1209},"obj":"Protein"},{"id":"T18717","span":{"begin":1333,"end":1352},"obj":"Protein"}],"relations":[{"id":"R13932","pred":"themeOf","subj":"T18705","obj":"T18704"},{"id":"R13933","pred":"themeOf","subj":"T18711","obj":"T18712"}],"text":"Materials\nRecombinant human M-CSF was purchased from R\u0026D Systems (Minneapolis, MN). Ro-31-8220, Gö-6976, Rotterin, and BAPTA/AM were obtained from Calbiochem (San Diego, CA). Low endotoxin RPMI and X-vivo serum free medium were obtained from Lonza Walkersville Inc (Walkersville, MD). Fetal bovine serum (FBS, certified \u003c0.06 endotoxin units/ml endotoxin levels) was purchased from Atlanta Biological (Lawrenceville, GA). All other cell culture reagents were obtained through Invitrogen (Carlsbad, CA). PKCα NF-κB p65, Lamin B, β-actin, and GAPDH antibodies, anti-mouse and rabbit IgG-HRP conjugated antibodies, and PKCα siRNA were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Phospho-NFκB p65 (Ser276 or Ser536) antibodies were purchased from Cell Signaling Technology (Danvers, MA). DNA constructs pTAL-SEAP and pNF-κB-SEAP were obtained from Clontech (Mountian View, CA). Expression vectors containing CMV-p65 WT, CMV-p65 276S/A and CMV-p65 536S/A were cloned in pBa5e Puro vector or pFLAG-CMV-2 vector as described previously [49]. Kinase deficient CMV-PKCα (KD-PKCα) or wild type pCMV-PKCα (WT-PKCα) were generous gifts from Alexandra C. Newton (University of California, San Diego, CA). IκBα (NFKBIA) and GAPDH primers for real-time RT-PCR were obtained from SABiosciences (Frederick, MD). A pool of mouse or human PKCα specific siRNA were purchased from Santa Cruz Biotechnology (Santa cruz, CA)."}