PMC:3245220 / 29062-30574 JSONTXT

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T26640","span":{"begin":50,"end":53},"obj":"Q04206"},{"id":"T26641","span":{"begin":50,"end":53},"obj":"P21579"},{"id":"T26642","span":{"begin":230,"end":233},"obj":"P21579"},{"id":"T26643","span":{"begin":230,"end":233},"obj":"Q04206"},{"id":"T26644","span":{"begin":246,"end":249},"obj":"Q04206"},{"id":"T26645","span":{"begin":246,"end":249},"obj":"P21579"},{"id":"T26646","span":{"begin":425,"end":430},"obj":"P09603"},{"id":"T26647","span":{"begin":427,"end":430},"obj":"P04141"},{"id":"T26648","span":{"begin":500,"end":503},"obj":"P21579"},{"id":"T26649","span":{"begin":500,"end":503},"obj":"Q04206"},{"id":"T26650","span":{"begin":619,"end":622},"obj":"Q04206"},{"id":"T26651","span":{"begin":619,"end":622},"obj":"P21579"},{"id":"T26652","span":{"begin":633,"end":636},"obj":"Q04206"},{"id":"T26653","span":{"begin":633,"end":636},"obj":"P21579"},{"id":"T26654","span":{"begin":654,"end":657},"obj":"Q04206"},{"id":"T26655","span":{"begin":654,"end":657},"obj":"P21579"},{"id":"T26656","span":{"begin":920,"end":923},"obj":"Q04206"},{"id":"T26657","span":{"begin":920,"end":923},"obj":"P21579"},{"id":"T26658","span":{"begin":1040,"end":1043},"obj":"Q04206"},{"id":"T26659","span":{"begin":1040,"end":1043},"obj":"P21579"},{"id":"T26660","span":{"begin":1056,"end":1059},"obj":"Q04206"},{"id":"T26661","span":{"begin":1056,"end":1059},"obj":"P21579"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"10.1371/journal.pone.0028081.g008 Figure 8 NF-κB p65 Ser276 is essential in regulating NF-κB activity.\n(A) Raw 264.7 cell line was transiently transfected with pNF-κB-SEAP along with empty vector or plasmid encoding either NF-κB p65 WT or NF-κB p65 276S/A. The cells were transfected for 18-24 hours, serum starved for 4 hours, and then incubated with 10 µM of Ro-31-8220 for 30 minutes prior to treatment with 100 ng/ml of M-CSF for 2 hours and SEAP secretion in the medium was measured. (B) NF-κB p65−/− cell line was transiently transfected with pNF-κB-SEAP along with empty vector or plasmid encoding either NF-κB p65 WT, NF-κB p65 276S/A, or NF-κB p65 536S/A. The cells were cultured for 24 hours and then serum starved for 4 hours. Cells were then incubated in fresh DMEM medium for 2 hours and SEAP secretion in the medium was measured. The results shown are fold change over empty vector + pTAL-SEAP. (C) NF-κB p65−/− cell line was transiently transfected with pNF-κB-SEAP with either empty vector or plasmid encoding either NF-κB p65 WT or NF-κB p65 276S/A. The cells were transfected for 24 hours and serum starved for 4 hours, and then incubated with 10 µM of Ro-31-8220 for 30 minutes prior to treatment with 10 ng/ml of TNFα. The supernatant were collected after 2 hours of treatment and SEAP secretion in the medium was measured. The results shown are the fold change over empty vector + pNF-κB-SEAP. Data shown are mean ± S.E.M for at least three independent experiments performed in duplicate. "}

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T26662","span":{"begin":144,"end":155},"obj":"Gene_expression"},{"id":"T26663","span":{"begin":144,"end":155},"obj":"Gene_expression"},{"id":"T26664","span":{"begin":533,"end":544},"obj":"Gene_expression"},{"id":"T26665","span":{"begin":533,"end":544},"obj":"Gene_expression"},{"id":"T26666","span":{"begin":533,"end":544},"obj":"Gene_expression"},{"id":"T26667","span":{"begin":953,"end":964},"obj":"Gene_expression"},{"id":"T26668","span":{"begin":953,"end":964},"obj":"Gene_expression"},{"id":"T26669","span":{"begin":50,"end":53},"obj":"Protein"},{"id":"T26670","span":{"begin":1056,"end":1059},"obj":"Protein"},{"id":"T26671","span":{"begin":1234,"end":1238},"obj":"Protein"},{"id":"T26672","span":{"begin":230,"end":233},"obj":"Protein"},{"id":"T26673","span":{"begin":246,"end":249},"obj":"Protein"},{"id":"T26674","span":{"begin":500,"end":503},"obj":"Protein"},{"id":"T26675","span":{"begin":619,"end":622},"obj":"Protein"},{"id":"T26676","span":{"begin":633,"end":636},"obj":"Protein"},{"id":"T26677","span":{"begin":654,"end":657},"obj":"Protein"},{"id":"T26678","span":{"begin":920,"end":923},"obj":"Protein"},{"id":"T26679","span":{"begin":1040,"end":1043},"obj":"Protein"},{"id":"T26680","span":{"begin":425,"end":430},"obj":"Protein"}],"relations":[{"id":"R20419","pred":"themeOf","subj":"T26670","obj":"T26668"},{"id":"R20420","pred":"themeOf","subj":"T26672","obj":"T26662"},{"id":"R20421","pred":"themeOf","subj":"T26673","obj":"T26663"},{"id":"R20422","pred":"themeOf","subj":"T26675","obj":"T26664"},{"id":"R20423","pred":"themeOf","subj":"T26676","obj":"T26665"},{"id":"R20424","pred":"themeOf","subj":"T26677","obj":"T26666"},{"id":"R20425","pred":"themeOf","subj":"T26679","obj":"T26667"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"10.1371/journal.pone.0028081.g008 Figure 8 NF-κB p65 Ser276 is essential in regulating NF-κB activity.\n(A) Raw 264.7 cell line was transiently transfected with pNF-κB-SEAP along with empty vector or plasmid encoding either NF-κB p65 WT or NF-κB p65 276S/A. The cells were transfected for 18-24 hours, serum starved for 4 hours, and then incubated with 10 µM of Ro-31-8220 for 30 minutes prior to treatment with 100 ng/ml of M-CSF for 2 hours and SEAP secretion in the medium was measured. (B) NF-κB p65−/− cell line was transiently transfected with pNF-κB-SEAP along with empty vector or plasmid encoding either NF-κB p65 WT, NF-κB p65 276S/A, or NF-κB p65 536S/A. The cells were cultured for 24 hours and then serum starved for 4 hours. Cells were then incubated in fresh DMEM medium for 2 hours and SEAP secretion in the medium was measured. The results shown are fold change over empty vector + pTAL-SEAP. (C) NF-κB p65−/− cell line was transiently transfected with pNF-κB-SEAP with either empty vector or plasmid encoding either NF-κB p65 WT or NF-κB p65 276S/A. The cells were transfected for 24 hours and serum starved for 4 hours, and then incubated with 10 µM of Ro-31-8220 for 30 minutes prior to treatment with 10 ng/ml of TNFα. The supernatant were collected after 2 hours of treatment and SEAP secretion in the medium was measured. The results shown are the fold change over empty vector + pNF-κB-SEAP. Data shown are mean ± S.E.M for at least three independent experiments performed in duplicate. "}

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T26801","span":{"begin":44,"end":49},"obj":"Protein"},{"id":"T26802","span":{"begin":50,"end":53},"obj":"Protein"},{"id":"T26803","span":{"begin":88,"end":93},"obj":"Protein"},{"id":"T26804","span":{"begin":77,"end":87},"obj":"Regulation"},{"id":"T26805","span":{"begin":161,"end":167},"obj":"Protein"},{"id":"T26806","span":{"begin":168,"end":172},"obj":"Protein"},{"id":"T26807","span":{"begin":224,"end":229},"obj":"Protein"},{"id":"T26808","span":{"begin":230,"end":236},"obj":"Protein"},{"id":"T26809","span":{"begin":240,"end":245},"obj":"Protein"},{"id":"T26810","span":{"begin":246,"end":256},"obj":"Protein"},{"id":"T26811","span":{"begin":144,"end":155},"obj":"Gene_expression"},{"id":"T26812","span":{"begin":425,"end":430},"obj":"Protein"},{"id":"T26813","span":{"begin":447,"end":451},"obj":"Protein"},{"id":"T26814","span":{"begin":452,"end":461},"obj":"Localization"},{"id":"T26815","span":{"begin":494,"end":499},"obj":"Protein"},{"id":"T26816","span":{"begin":550,"end":556},"obj":"Protein"},{"id":"T26817","span":{"begin":557,"end":561},"obj":"Protein"},{"id":"T26818","span":{"begin":613,"end":618},"obj":"Protein"},{"id":"T26819","span":{"begin":619,"end":625},"obj":"Protein"},{"id":"T26820","span":{"begin":627,"end":632},"obj":"Protein"},{"id":"T26821","span":{"begin":633,"end":643},"obj":"Protein"},{"id":"T26822","span":{"begin":648,"end":653},"obj":"Protein"},{"id":"T26823","span":{"begin":654,"end":664},"obj":"Protein"},{"id":"T26824","span":{"begin":533,"end":544},"obj":"Gene_expression"},{"id":"T26825","span":{"begin":802,"end":806},"obj":"Protein"},{"id":"T26826","span":{"begin":807,"end":816},"obj":"Localization"},{"id":"T26827","span":{"begin":914,"end":919},"obj":"Protein"},{"id":"T26828","span":{"begin":970,"end":976},"obj":"Protein"},{"id":"T26829","span":{"begin":977,"end":981},"obj":"Protein"},{"id":"T26830","span":{"begin":1034,"end":1039},"obj":"Protein"},{"id":"T26831","span":{"begin":1040,"end":1046},"obj":"Protein"},{"id":"T26832","span":{"begin":1050,"end":1055},"obj":"Protein"},{"id":"T26833","span":{"begin":1056,"end":1066},"obj":"Protein"},{"id":"T26834","span":{"begin":1234,"end":1238},"obj":"Protein"},{"id":"T26835","span":{"begin":1403,"end":1409},"obj":"Protein"},{"id":"T26836","span":{"begin":1410,"end":1414},"obj":"Protein"}],"relations":[{"id":"R20465","pred":"themeOf","subj":"T26803","obj":"T26804"},{"id":"R20466","pred":"themeOf","subj":"T26806","obj":"T26811"},{"id":"R20467","pred":"themeOf","subj":"T26813","obj":"T26814"},{"id":"R20468","pred":"themeOf","subj":"T26817","obj":"T26824"},{"id":"R20469","pred":"themeOf","subj":"T26825","obj":"T26826"}],"text":"10.1371/journal.pone.0028081.g008 Figure 8 NF-κB p65 Ser276 is essential in regulating NF-κB activity.\n(A) Raw 264.7 cell line was transiently transfected with pNF-κB-SEAP along with empty vector or plasmid encoding either NF-κB p65 WT or NF-κB p65 276S/A. The cells were transfected for 18-24 hours, serum starved for 4 hours, and then incubated with 10 µM of Ro-31-8220 for 30 minutes prior to treatment with 100 ng/ml of M-CSF for 2 hours and SEAP secretion in the medium was measured. (B) NF-κB p65−/− cell line was transiently transfected with pNF-κB-SEAP along with empty vector or plasmid encoding either NF-κB p65 WT, NF-κB p65 276S/A, or NF-κB p65 536S/A. The cells were cultured for 24 hours and then serum starved for 4 hours. Cells were then incubated in fresh DMEM medium for 2 hours and SEAP secretion in the medium was measured. The results shown are fold change over empty vector + pTAL-SEAP. (C) NF-κB p65−/− cell line was transiently transfected with pNF-κB-SEAP with either empty vector or plasmid encoding either NF-κB p65 WT or NF-κB p65 276S/A. The cells were transfected for 24 hours and serum starved for 4 hours, and then incubated with 10 µM of Ro-31-8220 for 30 minutes prior to treatment with 10 ng/ml of TNFα. The supernatant were collected after 2 hours of treatment and SEAP secretion in the medium was measured. The results shown are the fold change over empty vector + pNF-κB-SEAP. Data shown are mean ± S.E.M for at least three independent experiments performed in duplicate. "}

{"project":"events-check-again","denotations":[{"id":"T26782","span":{"begin":50,"end":53},"obj":"Protein"},{"id":"T26783","span":{"begin":144,"end":155},"obj":"Gene_expression"},{"id":"T26784","span":{"begin":144,"end":155},"obj":"Gene_expression"},{"id":"T26785","span":{"begin":230,"end":233},"obj":"Protein"},{"id":"T26786","span":{"begin":246,"end":249},"obj":"Protein"},{"id":"T26787","span":{"begin":425,"end":430},"obj":"Protein"},{"id":"T26788","span":{"begin":500,"end":503},"obj":"Protein"},{"id":"T26789","span":{"begin":533,"end":544},"obj":"Gene_expression"},{"id":"T26790","span":{"begin":533,"end":544},"obj":"Gene_expression"},{"id":"T26791","span":{"begin":533,"end":544},"obj":"Gene_expression"},{"id":"T26792","span":{"begin":619,"end":622},"obj":"Protein"},{"id":"T26793","span":{"begin":633,"end":636},"obj":"Protein"},{"id":"T26794","span":{"begin":654,"end":657},"obj":"Protein"},{"id":"T26795","span":{"begin":920,"end":923},"obj":"Protein"},{"id":"T26796","span":{"begin":953,"end":964},"obj":"Gene_expression"},{"id":"T26797","span":{"begin":953,"end":964},"obj":"Gene_expression"},{"id":"T26798","span":{"begin":1040,"end":1043},"obj":"Protein"},{"id":"T26799","span":{"begin":1056,"end":1059},"obj":"Protein"},{"id":"T26800","span":{"begin":1234,"end":1238},"obj":"Protein"}],"relations":[{"id":"R20458","pred":"themeOf","subj":"T26785","obj":"T26783"},{"id":"R20459","pred":"themeOf","subj":"T26786","obj":"T26784"},{"id":"R20460","pred":"themeOf","subj":"T26792","obj":"T26789"},{"id":"R20461","pred":"themeOf","subj":"T26793","obj":"T26790"},{"id":"R20462","pred":"themeOf","subj":"T26794","obj":"T26791"},{"id":"R20463","pred":"themeOf","subj":"T26798","obj":"T26796"},{"id":"R20464","pred":"themeOf","subj":"T26799","obj":"T26797"}],"text":"10.1371/journal.pone.0028081.g008 Figure 8 NF-κB p65 Ser276 is essential in regulating NF-κB activity.\n(A) Raw 264.7 cell line was transiently transfected with pNF-κB-SEAP along with empty vector or plasmid encoding either NF-κB p65 WT or NF-κB p65 276S/A. The cells were transfected for 18-24 hours, serum starved for 4 hours, and then incubated with 10 µM of Ro-31-8220 for 30 minutes prior to treatment with 100 ng/ml of M-CSF for 2 hours and SEAP secretion in the medium was measured. (B) NF-κB p65−/− cell line was transiently transfected with pNF-κB-SEAP along with empty vector or plasmid encoding either NF-κB p65 WT, NF-κB p65 276S/A, or NF-κB p65 536S/A. The cells were cultured for 24 hours and then serum starved for 4 hours. Cells were then incubated in fresh DMEM medium for 2 hours and SEAP secretion in the medium was measured. The results shown are fold change over empty vector + pTAL-SEAP. (C) NF-κB p65−/− cell line was transiently transfected with pNF-κB-SEAP with either empty vector or plasmid encoding either NF-κB p65 WT or NF-κB p65 276S/A. The cells were transfected for 24 hours and serum starved for 4 hours, and then incubated with 10 µM of Ro-31-8220 for 30 minutes prior to treatment with 10 ng/ml of TNFα. The supernatant were collected after 2 hours of treatment and SEAP secretion in the medium was measured. The results shown are the fold change over empty vector + pNF-κB-SEAP. Data shown are mean ± S.E.M for at least three independent experiments performed in duplicate. "}

{"project":"GO-CC","denotations":[{"id":"T26691","span":{"begin":118,"end":122},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T26692","span":{"begin":507,"end":511},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T26693","span":{"begin":927,"end":931},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T26694","span":{"begin":1072,"end":1077},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"10.1371/journal.pone.0028081.g008 Figure 8 NF-κB p65 Ser276 is essential in regulating NF-κB activity.\n(A) Raw 264.7 cell line was transiently transfected with pNF-κB-SEAP along with empty vector or plasmid encoding either NF-κB p65 WT or NF-κB p65 276S/A. The cells were transfected for 18-24 hours, serum starved for 4 hours, and then incubated with 10 µM of Ro-31-8220 for 30 minutes prior to treatment with 100 ng/ml of M-CSF for 2 hours and SEAP secretion in the medium was measured. (B) NF-κB p65−/− cell line was transiently transfected with pNF-κB-SEAP along with empty vector or plasmid encoding either NF-κB p65 WT, NF-κB p65 276S/A, or NF-κB p65 536S/A. The cells were cultured for 24 hours and then serum starved for 4 hours. Cells were then incubated in fresh DMEM medium for 2 hours and SEAP secretion in the medium was measured. The results shown are fold change over empty vector + pTAL-SEAP. (C) NF-κB p65−/− cell line was transiently transfected with pNF-κB-SEAP with either empty vector or plasmid encoding either NF-κB p65 WT or NF-κB p65 276S/A. The cells were transfected for 24 hours and serum starved for 4 hours, and then incubated with 10 µM of Ro-31-8220 for 30 minutes prior to treatment with 10 ng/ml of TNFα. The supernatant were collected after 2 hours of treatment and SEAP secretion in the medium was measured. The results shown are the fold change over empty vector + pNF-κB-SEAP. Data shown are mean ± S.E.M for at least three independent experiments performed in duplicate. "}

{"project":"GO-BP","denotations":[{"id":"T26688","span":{"begin":452,"end":461},"obj":"http://purl.obolibrary.org/obo/GO_0046903"},{"id":"T26689","span":{"begin":807,"end":816},"obj":"http://purl.obolibrary.org/obo/GO_0046903"},{"id":"T26690","span":{"begin":1307,"end":1316},"obj":"http://purl.obolibrary.org/obo/GO_0046903"}],"text":"10.1371/journal.pone.0028081.g008 Figure 8 NF-κB p65 Ser276 is essential in regulating NF-κB activity.\n(A) Raw 264.7 cell line was transiently transfected with pNF-κB-SEAP along with empty vector or plasmid encoding either NF-κB p65 WT or NF-κB p65 276S/A. The cells were transfected for 18-24 hours, serum starved for 4 hours, and then incubated with 10 µM of Ro-31-8220 for 30 minutes prior to treatment with 100 ng/ml of M-CSF for 2 hours and SEAP secretion in the medium was measured. (B) NF-κB p65−/− cell line was transiently transfected with pNF-κB-SEAP along with empty vector or plasmid encoding either NF-κB p65 WT, NF-κB p65 276S/A, or NF-κB p65 536S/A. The cells were cultured for 24 hours and then serum starved for 4 hours. Cells were then incubated in fresh DMEM medium for 2 hours and SEAP secretion in the medium was measured. The results shown are fold change over empty vector + pTAL-SEAP. (C) NF-κB p65−/− cell line was transiently transfected with pNF-κB-SEAP with either empty vector or plasmid encoding either NF-κB p65 WT or NF-κB p65 276S/A. The cells were transfected for 24 hours and serum starved for 4 hours, and then incubated with 10 µM of Ro-31-8220 for 30 minutes prior to treatment with 10 ng/ml of TNFα. The supernatant were collected after 2 hours of treatment and SEAP secretion in the medium was measured. The results shown are the fold change over empty vector + pNF-κB-SEAP. Data shown are mean ± S.E.M for at least three independent experiments performed in duplicate. "}

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Figure 8 NF-κB p65 Ser276 is essential in regulating NF-κB activity.\n(A) Raw 264.7 cell line was transiently transfected with pNF-κB-SEAP along with empty vector or plasmid encoding either NF-κB p65 WT or NF-κB p65 276S/A. The cells were transfected for 18-24 hours, serum starved for 4 hours, and then incubated with 10 µM of Ro-31-8220 for 30 minutes prior to treatment with 100 ng/ml of M-CSF for 2 hours and SEAP secretion in the medium was measured. (B) NF-κB p65−/− cell line was transiently transfected with pNF-κB-SEAP along with empty vector or plasmid encoding either NF-κB p65 WT, NF-κB p65 276S/A, or NF-κB p65 536S/A. The cells were cultured for 24 hours and then serum starved for 4 hours. Cells were then incubated in fresh DMEM medium for 2 hours and SEAP secretion in the medium was measured. The results shown are fold change over empty vector + pTAL-SEAP. (C) NF-κB p65−/− cell line was transiently transfected with pNF-κB-SEAP with either empty vector or plasmid encoding either NF-κB p65 WT or NF-κB p65 276S/A. The cells were transfected for 24 hours and serum starved for 4 hours, and then incubated with 10 µM of Ro-31-8220 for 30 minutes prior to treatment with 10 ng/ml of TNFα. The supernatant were collected after 2 hours of treatment and SEAP secretion in the medium was measured. The results shown are the fold change over empty vector + pNF-κB-SEAP. Data shown are mean ± S.E.M for at least three independent experiments performed in duplicate. "}

{"project":"test2","denotations":[{"id":"T26340","span":{"begin":50,"end":53},"obj":"Protein"},{"id":"T26341","span":{"begin":230,"end":233},"obj":"Protein"},{"id":"T26342","span":{"begin":246,"end":249},"obj":"Protein"},{"id":"T26343","span":{"begin":425,"end":430},"obj":"Protein"},{"id":"T26344","span":{"begin":500,"end":503},"obj":"Protein"},{"id":"T26345","span":{"begin":619,"end":622},"obj":"Protein"},{"id":"T26346","span":{"begin":633,"end":636},"obj":"Protein"},{"id":"T26347","span":{"begin":654,"end":657},"obj":"Protein"},{"id":"T26348","span":{"begin":920,"end":923},"obj":"Protein"},{"id":"T26349","span":{"begin":1040,"end":1043},"obj":"Protein"},{"id":"T26350","span":{"begin":1056,"end":1059},"obj":"Protein"},{"id":"T26351","span":{"begin":1234,"end":1238},"obj":"Protein"}],"text":"10.1371/journal.pone.0028081.g008 Figure 8 NF-κB p65 Ser276 is essential in regulating NF-κB activity.\n(A) Raw 264.7 cell line was transiently transfected with pNF-κB-SEAP along with empty vector or plasmid encoding either NF-κB p65 WT or NF-κB p65 276S/A. The cells were transfected for 18-24 hours, serum starved for 4 hours, and then incubated with 10 µM of Ro-31-8220 for 30 minutes prior to treatment with 100 ng/ml of M-CSF for 2 hours and SEAP secretion in the medium was measured. (B) NF-κB p65−/− cell line was transiently transfected with pNF-κB-SEAP along with empty vector or plasmid encoding either NF-κB p65 WT, NF-κB p65 276S/A, or NF-κB p65 536S/A. The cells were cultured for 24 hours and then serum starved for 4 hours. Cells were then incubated in fresh DMEM medium for 2 hours and SEAP secretion in the medium was measured. The results shown are fold change over empty vector + pTAL-SEAP. (C) NF-κB p65−/− cell line was transiently transfected with pNF-κB-SEAP with either empty vector or plasmid encoding either NF-κB p65 WT or NF-κB p65 276S/A. The cells were transfected for 24 hours and serum starved for 4 hours, and then incubated with 10 µM of Ro-31-8220 for 30 minutes prior to treatment with 10 ng/ml of TNFα. The supernatant were collected after 2 hours of treatment and SEAP secretion in the medium was measured. The results shown are the fold change over empty vector + pNF-κB-SEAP. Data shown are mean ± S.E.M for at least three independent experiments performed in duplicate. "}