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PMC:3245220 / 17454-19577 JSONTXT

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bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T7750 0-5 P09603 denotes M-CSF
T7751 2-5 P04141 denotes CSF
T7752 72-75 Q04206 denotes p65
T7753 72-75 P21579 denotes p65
T7754 171-175 P25963 denotes IκBα
T7755 238-241 P19838 denotes p50
T7756 242-245 P21579 denotes p65
T7757 242-245 Q04206 denotes p65
T7758 360-365 P09603 denotes M-CSF
T7759 362-365 P04141 denotes CSF
T7760 421-425 P25963 denotes IκBα
T7761 536-540 P25963 denotes IκBα
T7762 644-649 P09603 denotes M-CSF
T7763 646-649 P04141 denotes CSF
T7764 658-662 P25963 denotes IκBα
T7765 692-697 P09603 denotes M-CSF
T7766 694-697 P04141 denotes CSF
T7767 833-836 Q04206 denotes p65
T7768 833-836 P21579 denotes p65
T24908 882-887 P09603 denotes M-CSF
T24909 884-887 P04141 denotes CSF
T24910 927-931 P25963 denotes IκBα
T24911 987-990 P21579 denotes p65
T24912 987-990 Q04206 denotes p65
T24913 1117-1122 P09603 denotes M-CSF
T24914 1119-1122 P04141 denotes CSF
T24915 1224-1228 P25963 denotes IκBα
T24916 1394-1399 P09603 denotes M-CSF
T24917 1396-1399 P04141 denotes CSF
T24918 1507-1510 P21579 denotes p65
T24919 1507-1510 Q04206 denotes p65
T24920 1585-1588 P21579 denotes p65
T24921 1585-1588 Q04206 denotes p65
T24922 1705-1710 P09603 denotes M-CSF
T24923 1707-1710 P04141 denotes CSF
T24924 1795-1798 Q04206 denotes p65
T24925 1795-1798 P21579 denotes p65
T24926 2021-2026 P04406 denotes GAPDH

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue Negation Speculation
T7769 43-51 Regulation denotes Regulate true
T7770 650-657 Positive_regulation denotes induced
T7771 663-674 Protein_catabolism denotes degradation
T7772 719-728 Regulation denotes augmented
T7773 792-823 Protein_modification denotes post-translational modification
T7774 76-86 Positive_regulation denotes Activation
T7775 136-151 Phosphorylation denotes phosphorylation
T7776 156-167 Protein_catabolism denotes degradation
T7777 426-437 Protein_catabolism denotes degradation
T7778 442-451 Regulation denotes regulated true
T7779 507-514 Negative_regulation denotes inhibit
T7780 523-532 Gene_expression denotes synthesis
T7781 638-643 Regulation denotes alter true
T7782 72-75 Protein denotes p65
T7783 171-175 Protein denotes IκBα
T7784 238-241 Protein denotes p50
T7785 242-245 Protein denotes p65
T7786 421-425 Protein denotes IκBα
T7787 536-540 Protein denotes IκBα
T7788 658-662 Protein denotes IκBα
T7789 833-836 Protein denotes p65
T7790 0-5 Protein denotes M-CSF
T7791 360-365 Protein denotes M-CSF
T7792 644-649 Protein denotes M-CSF
T7793 692-697 Protein denotes M-CSF
T24927 918-926 Regulation denotes regulate true
T24928 932-943 Protein_catabolism denotes degradation
T24929 948-957 Regulation denotes regulates
T24930 962-977 Phosphorylation denotes phosphorylation
T24931 1465-1472 Phosphorylation denotes phospho true
T24932 1483-1490 Phosphorylation denotes phospho true
T24933 927-931 Protein denotes IκBα
T24934 1473-1479 Entity denotes Ser276
T24935 1491-1497 Entity denotes Ser536
T24936 987-990 Protein denotes p65
T24937 1507-1510 Protein denotes p65
T24938 1585-1588 Protein denotes p65
T24939 994-1000 Entity denotes Ser276
T24940 882-887 Protein denotes M-CSF
T24941 1117-1122 Protein denotes M-CSF
T24942 1394-1399 Protein denotes M-CSF
T24943 1705-1710 Protein denotes M-CSF
T24944 1682-1689 Negative_regulation denotes absence
T24945 1693-1701 Positive_regulation denotes presence
R5796 T7770 T7781 themeOf induced,alter
R5797 T7771 T7770 themeOf degradation,induced
R5798 T7773 T7772 themeOf post-translational modification,augmented
R5799 T7774 T7769 themeOf Activation,Regulate
R5800 T7777 T7778 themeOf degradation,regulated
R5801 T7780 T7779 themeOf synthesis,inhibit
R5802 T7782 T7774 themeOf p65,Activation
R5803 T7783 T7775 themeOf IκBα,phosphorylation
R5804 T7783 T7776 themeOf IκBα,degradation
R5805 T7786 T7777 themeOf IκBα,degradation
R5806 T7787 T7780 themeOf IκBα,synthesis
R5807 T7788 T7771 themeOf IκBα,degradation
R5808 T7789 T7773 themeOf p65,post-translational modification
R5809 T7792 T7770 causeOf M-CSF,induced
R19084 T24928 T24927 themeOf degradation,regulate
R19085 T24930 T24929 themeOf phosphorylation,regulates
R19086 T24933 T24928 themeOf IκBα,degradation
R19087 T24934 T24931 themeOf Ser276,phospho
R19088 T24934 T24937 partOf Ser276,p65
R19089 T24935 T24932 themeOf Ser536,phospho
R19090 T24935 T24937 partOf Ser536,p65
R19091 T24939 T24930 themeOf Ser276,phosphorylation
R19092 T24939 T24936 partOf Ser276,p65
R19093 T24940 T24927 causeOf M-CSF,regulate
R19094 T24940 T24929 causeOf M-CSF,regulates
R19095 T24943 T24945 themeOf M-CSF,presence
R19096 T24943 T24944 themeOf M-CSF,absence

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T7959 0-5 Protein denotes M-CSF
T7960 30-33 Protein denotes PKC
T7961 66-75 Protein denotes NF-κB p65
T7962 16-26 Positive_regulation denotes Activation
T7963 43-51 Regulation denotes Regulate
T7964 119-124 Protein denotes NF-κB
T7965 171-175 Protein denotes IκBα
T7966 232-237 Protein denotes NF-κB
T7967 238-241 Protein denotes p50
T7968 242-245 Protein denotes p65
T7969 105-115 Positive_regulation denotes activation
T7970 136-151 Phosphorylation denotes phosphorylation
T7971 203-210 Entity denotes nuclear
T7972 211-224 Localization denotes translocation
T7973 211-224 Localization denotes translocation
T7974 211-224 Localization denotes translocation
T7975 319-322 Protein denotes PKC
T7976 374-379 Protein denotes NF-κB
T7977 421-441 Protein denotes IκBα degradation was
T7978 455-458 Protein denotes PKC
T7979 380-390 Positive_regulation denotes activation
T7980 442-451 Regulation denotes regulated
T7981 349-359 Regulation denotes regulating
T7982 536-540 Protein denotes IκBα
T7983 523-532 Gene_expression denotes synthesis
T7984 507-514 Negative_regulation denotes inhibit
T7985 599-602 Protein denotes PKC
T7986 644-649 Protein denotes M-CSF
T7987 658-662 Protein denotes IκBα
T7988 692-697 Protein denotes M-CSF
T7989 706-709 Protein denotes PKC
T7990 729-734 Protein denotes NF-κB
T7991 827-836 Protein denotes NF-κB p65
T7992 603-613 Negative_regulation denotes inhibition
T7993 663-674 Protein_catabolism denotes degradation
T7994 698-705 Positive_regulation denotes induced
T7995 811-823 Positive_regulation denotes modification
T7996 638-643 Positive_regulation denotes alter
T7997 650-657 Positive_regulation denotes induced
T25079 882-887 Protein denotes M-CSF
T25080 896-899 Protein denotes PKC
T25081 927-947 Protein denotes IκBα degradation but
T25082 981-990 Protein denotes NF-κB p65
T25083 888-895 Positive_regulation denotes induced
T25084 918-926 Regulation denotes regulate
T25085 948-957 Regulation denotes regulates
T25086 962-977 Phosphorylation denotes phosphorylation
T25087 1117-1122 Protein denotes M-CSF
T25088 1219-1237 Protein denotes anti-IκBα antibody
T25089 1394-1399 Protein denotes M-CSF
T25090 1501-1510 Protein denotes NF-κB p65
T25091 1579-1588 Protein denotes NF-κB p65
T25092 1705-1710 Protein denotes M-CSF
T25093 1789-1809 Protein denotes NF-κB p65 antibodies
T25094 1919-1932 Protein denotes phospho-NF-κB
T25095 2021-2026 Protein denotes GAPDH
T25096 2031-2038 Protein denotes Lamin B
R5900 T7960 T7962 themeOf PKC,Activation
R5901 T7961 T7963 themeOf NF-κB p65,Regulate
R5902 T7964 T7969 themeOf NF-κB,activation
R5903 T7965 T7970 themeOf IκBα,phosphorylation
R5904 T7966 T7972 themeOf NF-κB,translocation
R5905 T7967 T7973 themeOf p50,translocation
R5906 T7968 T7974 themeOf p65,translocation
R5907 T7971 T7972 locationOf nuclear,translocation
R5908 T7971 T7973 locationOf nuclear,translocation
R5909 T7971 T7974 locationOf nuclear,translocation
R5910 T7976 T7979 themeOf NF-κB,activation
R5911 T7977 T7980 themeOf IκBα degradation was,regulated
R5912 T7978 T7980 causeOf PKC,regulated
R5913 T7979 T7981 themeOf activation,regulating
R5914 T7982 T7983 themeOf IκBα,synthesis
R5915 T7983 T7984 themeOf synthesis,inhibit
R5916 T7985 T7992 themeOf PKC,inhibition
R5917 T7986 T7997 causeOf M-CSF,induced
R5918 T7987 T7993 themeOf IκBα,degradation
R5919 T7988 T7994 causeOf M-CSF,induced
R5920 T7989 T7994 themeOf PKC,induced
R5921 T7991 T7995 themeOf NF-κB p65,modification
R5922 T7992 T7996 causeOf inhibition,alter
R5923 T7993 T7996 themeOf degradation,alter
R5924 T7993 T7997 themeOf degradation,induced
R19165 T25079 T25083 causeOf M-CSF,induced
R19166 T25080 T25083 themeOf PKC,induced
R19167 T25081 T25084 themeOf IκBα degradation but,regulate
R19168 T25082 T25086 themeOf NF-κB p65,phosphorylation
R19169 T25083 T25085 themeOf induced,regulates

events-check-again

Id Subject Object Predicate Lexical cue Negation Speculation
T7934 0-5 Protein denotes M-CSF
T7935 43-51 Regulation denotes Regulate true
T7936 72-75 Protein denotes p65
T7937 76-86 Positive_regulation denotes Activation
T7938 136-151 Phosphorylation denotes phosphorylation
T7939 156-167 Protein_catabolism denotes degradation
T7940 171-175 Protein denotes IκBα
T7941 238-241 Protein denotes p50
T7942 242-245 Protein denotes p65
T7943 360-365 Protein denotes M-CSF
T7944 421-425 Protein denotes IκBα
T7945 426-437 Protein_catabolism denotes degradation
T7946 442-451 Regulation denotes regulated true
T7947 507-514 Negative_regulation denotes inhibit
T7948 523-532 Gene_expression denotes synthesis
T7949 536-540 Protein denotes IκBα
T7950 638-643 Regulation denotes alter true
T7951 644-649 Protein denotes M-CSF
T7952 650-657 Positive_regulation denotes induced
T7953 658-662 Protein denotes IκBα
T7954 663-674 Protein_catabolism denotes degradation
T7955 692-697 Protein denotes M-CSF
T7956 719-728 Regulation denotes augmented
T7957 792-823 Protein_modification denotes post-translational modification
T7958 833-836 Protein denotes p65
T25060 882-887 Protein denotes M-CSF
T25061 918-926 Regulation denotes regulate true
T25062 927-931 Protein denotes IκBα
T25063 932-943 Protein_catabolism denotes degradation
T25064 948-957 Regulation denotes regulates
T25065 962-977 Phosphorylation denotes phosphorylation
T25066 987-990 Protein denotes p65
T25067 994-1000 Entity denotes Ser276
T25068 1117-1122 Protein denotes M-CSF
T25069 1394-1399 Protein denotes M-CSF
T25070 1465-1472 Phosphorylation denotes phospho true
T25071 1473-1479 Entity denotes Ser276
T25072 1483-1490 Phosphorylation denotes phospho true
T25073 1491-1497 Entity denotes Ser536
T25074 1507-1510 Protein denotes p65
T25075 1585-1588 Protein denotes p65
T25076 1682-1689 Negative_regulation denotes absence
T25077 1693-1701 Positive_regulation denotes presence
T25078 1705-1710 Protein denotes M-CSF
R5886 T7936 T7937 themeOf p65,Activation
R5887 T7937 T7935 themeOf Activation,Regulate
R5888 T7940 T7938 themeOf IκBα,phosphorylation
R5889 T7940 T7939 themeOf IκBα,degradation
R5890 T7944 T7945 themeOf IκBα,degradation
R5891 T7945 T7946 themeOf degradation,regulated
R5892 T7948 T7947 themeOf synthesis,inhibit
R5893 T7949 T7948 themeOf IκBα,synthesis
R5894 T7951 T7952 causeOf M-CSF,induced
R5895 T7952 T7950 themeOf induced,alter
R5896 T7953 T7954 themeOf IκBα,degradation
R5897 T7954 T7952 themeOf degradation,induced
R5898 T7957 T7956 themeOf post-translational modification,augmented
R5899 T7958 T7957 themeOf p65,post-translational modification
R19152 T25060 T25061 causeOf M-CSF,regulate
R19153 T25060 T25064 causeOf M-CSF,regulates
R19154 T25062 T25063 themeOf IκBα,degradation
R19155 T25063 T25061 themeOf degradation,regulate
R19156 T25065 T25064 themeOf phosphorylation,regulates
R19157 T25067 T25065 themeOf Ser276,phosphorylation
R19158 T25067 T25066 partOf Ser276,p65
R19159 T25071 T25070 themeOf Ser276,phospho
R19160 T25071 T25074 partOf Ser276,p65
R19161 T25073 T25072 themeOf Ser536,phospho
R19162 T25073 T25074 partOf Ser536,p65
R19163 T25078 T25077 themeOf M-CSF,presence
R19164 T25078 T25076 themeOf M-CSF,absence

GO-CC

Id Subject Object Predicate Lexical cue
T24957 1166-1170 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T24958 1422-1426 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T24959 1600-1604 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T24960 1626-1631 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T24961 1891-1896 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T24962 1229-1237 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T24963 1547-1557 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T24964 1799-1809 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T24965 1229-1237 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T24966 1547-1557 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T24967 1799-1809 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies

GO-MF

Id Subject Object Predicate Lexical cue
T7799 30-33 http://purl.obolibrary.org/obo/GO_0004697 denotes PKC
T7800 319-322 http://purl.obolibrary.org/obo/GO_0004697 denotes PKC
T7801 455-458 http://purl.obolibrary.org/obo/GO_0004697 denotes PKC
T7802 599-602 http://purl.obolibrary.org/obo/GO_0004697 denotes PKC
T7803 319-331 http://purl.obolibrary.org/obo/GO_0004697 denotes PKC activity
T7804 706-718 http://purl.obolibrary.org/obo/GO_0004697 denotes PKC activity
T24953 896-908 http://purl.obolibrary.org/obo/GO_0004697 denotes PKC activity
T24954 1229-1237 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T24955 1547-1557 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T24956 1799-1809 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies

GO-BP

Id Subject Object Predicate Lexical cue
T7998 156-167 http://purl.obolibrary.org/obo/GO_0009056 denotes degradation
T7999 426-437 http://purl.obolibrary.org/obo/GO_0009056 denotes degradation
T8000 550-561 http://purl.obolibrary.org/obo/GO_0009056 denotes degradation
T8001 663-674 http://purl.obolibrary.org/obo/GO_0009056 denotes degradation
T8002 515-532 http://purl.obolibrary.org/obo/GO_0006412 denotes protein synthesis
T8003 523-532 http://purl.obolibrary.org/obo/GO_0009058 denotes synthesis
T8004 735-750 http://purl.obolibrary.org/obo/GO_0006351 denotes transcriptional
T8005 792-823 http://purl.obolibrary.org/obo/GO_0043687 denotes post-translational modification
T8006 792-823 http://purl.obolibrary.org/obo/GO_1901873 denotes post-translational modification
T8007 792-823 http://purl.obolibrary.org/obo/GO_1901874 denotes post-translational modification
T8008 792-823 http://purl.obolibrary.org/obo/GO_1901875 denotes post-translational modification
T8009 105-124 http://purl.obolibrary.org/obo/GO_0051092 denotes activation of NF-κB
T8010 374-390 http://purl.obolibrary.org/obo/GO_0051092 denotes NF-κB activation
T8011 136-151 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T25097 932-943 http://purl.obolibrary.org/obo/GO_0009056 denotes degradation
T25098 962-977 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation

bionlp-st-ge-2016-coref

Id Subject Object Predicate Lexical cue
T7748 763-785 Anaphor denotes an alternative pathway
T7749 792-823 Antecedent denotes post-translational modification
R5795 T7748 T7749 boundBy an alternative pathway,post-translational modification

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T7622 0-15 JJ denotes M-CSF-Dependent
T7623 16-26 NN denotes Activation
T7624 27-29 IN denotes of
T7625 30-33 NN denotes PKC
T7626 34-38 VB denotes Does
T7627 39-42 RB denotes Not
T7628 43-51 VB denotes Regulate
T7629 52-55 DT denotes the
T7630 56-65 JJ denotes Classical
T7631 66-71 NN denotes NF-κB
T7632 72-75 NN denotes p65
T7633 76-86 NN denotes Activation
T7634 87-94 NN denotes Pathway
T7635 95-104 JJ denotes Canonical
T7636 105-115 NN denotes activation
T7637 116-118 IN denotes of
T7638 119-124 NN denotes NF-κB
T7639 125-131 VB denotes occurs
T7640 132-135 IN denotes via
T7641 136-151 NN denotes phosphorylation
T7642 152-155 CC denotes and
T7643 156-167 NN denotes degradation
T7644 168-170 IN denotes of
T7645 171-175 NN denotes IκBα
T7646 176-183 VB denotes leading
T7647 184-186 TO denotes to
T7648 187-190 DT denotes the
T7649 191-198 NN denotes release
T7650 199-202 CC denotes and
T7651 203-210 JJ denotes nuclear
T7652 211-224 NN denotes translocation
T7653 225-227 IN denotes of
T7654 228-231 DT denotes the
T7655 232-237 NN denotes NF-κB
T7656 238-245 NN denotes p50/p65
T7657 246-257 NN denotes heterodimer
T7658 258-260 TO denotes to
T7659 261-274 VB denotes transactivate
T7660 275-281 NN denotes target
T7661 282-287 NN denotes genes
T7662 288-289 -LRB- denotes [
T7663 289-291 CD denotes 37
T7664 291-292 -RRB- denotes ]
T7665 292-293 -COMMA- denotes ,
T7666 294-295 -LRB- denotes [
T7667 295-297 CD denotes 39
T7668 297-298 -RRB- denotes ]
T7669 300-305 IN denotes Since
T7670 306-318 JJ denotes conventional
T7671 319-322 NN denotes PKC
T7672 323-331 NN denotes activity
T7673 332-335 VB denotes was
T7674 336-345 JJ denotes important
T7675 346-348 IN denotes in
T7676 349-359 VB denotes regulating
T7677 360-373 JJ denotes M-CSF-induced
T7678 374-379 NN denotes NF-κB
T7679 380-390 NN denotes activation
T7680 390-391 -COMMA- denotes ,
T7681 392-394 PRP denotes we
T7682 395-399 RB denotes next
T7683 400-412 VB denotes investigated
T7684 413-420 IN denotes whether
T7685 421-425 NN denotes IκBα
T7686 426-437 NN denotes degradation
T7687 438-441 VB denotes was
T7688 442-451 VB denotes regulated
T7689 452-454 IN denotes by
T7690 455-458 NN denotes PKC
T7691 460-465 NN denotes Cells
T7692 466-470 VB denotes were
T7693 471-478 VB denotes treated
T7694 479-483 IN denotes with
T7695 484-497 NN denotes cyclohexamide
T7696 498-499 -LRB- denotes (
T7697 499-502 NN denotes CHX
T7698 502-503 -RRB- denotes )
T7699 504-506 TO denotes to
T7700 507-514 VB denotes inhibit
T7701 515-522 NN denotes protein
T7702 523-532 NN denotes synthesis
T7703 533-535 IN denotes of
T7704 536-540 NNP denotes IκBα
T7705 540-541 -COMMA- denotes ,
T7706 542-545 CC denotes and
T7707 546-549 PRP-DOLLAR- denotes its
T7708 550-561 NN denotes degradation
T7709 562-565 VB denotes was
T7710 566-574 VB denotes followed
T7711 576-578 IN denotes As
T7712 579-584 VB denotes shown
T7713 585-587 IN denotes in
T7714 588-594 NN denotes Figure
T7715 595-597 NN denotes 5A
T7716 597-598 -COMMA- denotes ,
T7717 599-602 NN denotes PKC
T7718 603-613 NN denotes inhibition
T7719 614-618 IN denotes with
T7720 619-629 NN denotes Ro-31-8220
T7721 630-633 VB denotes did
T7722 634-637 RB denotes not
T7723 638-643 VB denotes alter
T7724 644-657 JJ denotes M-CSF-induced
T7725 658-662 NN denotes IκBα
T7726 663-674 NN denotes degradation
T7727 674-675 -COMMA- denotes ,
T7728 676-686 VB denotes suggesting
T7729 687-691 IN denotes that
T7730 692-705 JJ denotes M-CSF-induced
T7731 706-709 NN denotes PKC
T7732 710-718 NN denotes activity
T7733 719-728 VB denotes augmented
T7734 729-734 NN denotes NF-κB
T7735 735-750 JJ denotes transcriptional
T7736 751-759 NN denotes activity
T7737 760-762 IN denotes by
T7738 763-765 DT denotes an
T7739 766-777 JJ denotes alternative
T7740 778-785 NN denotes pathway
T7741 785-786 -COMMA- denotes ,
T7742 787-791 IN denotes like
T7743 792-810 JJ denotes post-translational
T7744 811-823 NN denotes modification
T7745 824-826 IN denotes of
T7746 827-832 NN denotes NF-κB
T7747 833-836 NN denotes p65
T24714 882-895 JJ denotes M-CSF-induced
T24715 896-899 NN denotes PKC
T24716 900-908 NN denotes activity
T24717 909-913 VB denotes does
T24718 914-917 RB denotes not
T24719 918-926 VB denotes regulate
T24720 927-931 NN denotes IκBα
T24721 932-943 NN denotes degradation
T24722 944-947 CC denotes but
T24723 948-957 VB denotes regulates
T24724 958-961 DT denotes the
T24725 962-977 NN denotes phosphorylation
T24726 978-980 IN denotes of
T24727 981-986 NN denotes NF-κB
T24728 987-990 NN denotes p65
T24729 991-993 IN denotes at
T24730 994-1000 NN denotes Ser276
T24731 1002-1003 -LRB- denotes (
T24732 1003-1004 NN denotes A
T24733 1004-1005 -RRB- denotes )
T24734 1006-1010 NN denotes MDMs
T24735 1011-1015 VB denotes were
T24736 1016-1026 VB denotes pretreated
T24737 1027-1031 IN denotes with
T24738 1032-1045 NN denotes cycloheximide
T24739 1046-1047 -LRB- denotes (
T24740 1047-1050 NN denotes CHX
T24741 1050-1051 -RRB- denotes )
T24742 1052-1054 IN denotes in
T24743 1055-1058 DT denotes the
T24744 1059-1066 NN denotes absence
T24745 1067-1069 CC denotes or
T24746 1070-1078 NN denotes presence
T24747 1079-1081 IN denotes of
T24748 1082-1092 NN denotes Ro-31-8220
T24749 1093-1096 IN denotes for
T24750 1097-1099 CD denotes 30
T24751 1100-1107 NN denotes minutes
T24752 1108-1113 RB denotes prior
T24753 1114-1116 TO denotes to
T24754 1117-1122 NN denotes M-CSF
T24755 1123-1134 NN denotes stimulation
T24756 1135-1138 IN denotes for
T24757 1139-1142 DT denotes the
T24758 1143-1152 JJ denotes indicated
T24759 1153-1158 NN denotes times
T24760 1160-1165 JJ denotes Whole
T24761 1166-1170 NN denotes cell
T24762 1171-1178 NN denotes lysates
T24763 1179-1183 VB denotes were
T24764 1184-1193 VB denotes subjected
T24765 1194-1196 TO denotes to
T24766 1197-1204 JJ denotes Western
T24767 1205-1213 NN denotes blotting
T24768 1214-1218 IN denotes with
T24769 1219-1228 JJ denotes anti-IκBα
T24770 1229-1237 NN denotes antibody
T24771 1239-1243 NN denotes Data
T24772 1244-1247 VB denotes are
T24773 1248-1262 JJ denotes representative
T24774 1263-1265 IN denotes of
T24775 1266-1271 CD denotes three
T24776 1272-1283 JJ denotes independent
T24777 1284-1296 NN denotes experiments.
T24778 1297-1298 -LRB- denotes (
T24779 1298-1299 NN denotes B
T24780 1299-1300 -RRB- denotes )
T24781 1301-1305 NN denotes MDMs
T24782 1306-1310 VB denotes were
T24783 1311-1321 VB denotes pretreated
T24784 1322-1326 IN denotes with
T24785 1327-1333 CC denotes either
T24786 1334-1341 NN denotes vehicle
T24787 1342-1344 CC denotes or
T24788 1345-1355 NN denotes Ro-31-8220
T24789 1356-1359 IN denotes for
T24790 1360-1362 CD denotes 30
T24791 1363-1370 NN denotes minutes
T24792 1371-1377 IN denotes before
T24793 1378-1381 DT denotes the
T24794 1382-1390 NN denotes addition
T24795 1391-1393 IN denotes of
T24796 1394-1399 NN denotes M-CSF
T24797 1400-1403 IN denotes for
T24798 1404-1406 CD denotes 10
T24799 1407-1414 NN denotes minutes
T24800 1416-1421 JJ denotes Whole
T24801 1422-1426 NN denotes cell
T24802 1427-1434 NN denotes lysates
T24803 1435-1439 VB denotes were
T24804 1440-1448 VB denotes resolved
T24805 1449-1451 IN denotes by
T24806 1452-1460 NN denotes SDS-PAGE
T24807 1461-1464 CC denotes and
T24808 1465-1479 NN denotes phospho-Ser276
T24809 1480-1482 CC denotes or
T24810 1483-1497 NN denotes phospho-Ser536
T24811 1498-1500 IN denotes of
T24812 1501-1506 NN denotes NF-κB
T24813 1507-1510 NN denotes p65
T24814 1511-1514 VB denotes was
T24815 1515-1523 VB denotes detected
T24816 1524-1529 VB denotes using
T24817 1530-1546 JJ denotes phospho-specific
T24818 1547-1557 NN denotes antibodies
T24819 1558-1560 TO denotes to
T24820 1561-1567 CC denotes either
T24821 1568-1575 NN denotes residue
T24822 1576-1578 IN denotes of
T24823 1579-1584 NN denotes NF-κB
T24824 1585-1589 NN denotes p65.
T24825 1590-1591 -LRB- denotes (
T24826 1591-1592 NN denotes C
T24827 1592-1593 -RRB- denotes )
T24828 1594-1599 JJ denotes Whole
T24829 1600-1604 NN denotes cell
T24830 1605-1612 NN denotes lysates
T24831 1613-1615 IN denotes of
T24832 1616-1619 NN denotes RAW
T24833 1620-1625 CD denotes 264.7
T24834 1626-1631 NN denotes cells
T24835 1632-1639 VB denotes treated
T24836 1640-1644 IN denotes with
T24837 1645-1652 NN denotes vehicle
T24838 1653-1660 NN denotes control
T24839 1661-1663 CC denotes or
T24840 1664-1674 NN denotes Ro-31-8220
T24841 1675-1677 IN denotes in
T24842 1678-1681 DT denotes the
T24843 1682-1689 NN denotes absence
T24844 1690-1692 CC denotes or
T24845 1693-1701 NN denotes presence
T24846 1702-1704 IN denotes of
T24847 1705-1710 NN denotes M-CSF
T24848 1711-1715 VB denotes were
T24849 1716-1725 VB denotes subjected
T24850 1726-1728 TO denotes to
T24851 1729-1736 JJ denotes Western
T24852 1737-1741 NN denotes blot
T24853 1742-1750 NN denotes analysis
T24854 1751-1755 IN denotes with
T24855 1756-1770 NN denotes phospho-Ser276
T24856 1771-1773 CC denotes or
T24857 1774-1788 NN denotes phospho-Ser536
T24858 1789-1794 NN denotes NF-κB
T24859 1795-1798 NN denotes p65
T24860 1799-1810 NN denotes antibodies.
T24861 1811-1812 -LRB- denotes (
T24862 1812-1813 NN denotes D
T24863 1813-1814 -RRB- denotes )
T24864 1815-1824 JJ denotes Cytosolic
T24865 1825-1828 CC denotes and
T24866 1829-1836 JJ denotes nuclear
T24867 1837-1846 NN denotes fractions
T24868 1847-1849 IN denotes of
T24869 1850-1853 NN denotes RAW
T24870 1854-1859 CD denotes 264.7
T24871 1860-1864 VB denotes were
T24872 1865-1873 VB denotes obtained
T24873 1874-1878 IN denotes from
T24874 1879-1882 DT denotes the
T24875 1883-1890 VB denotes treated
T24876 1891-1896 NN denotes cells
T24877 1897-1900 CC denotes and
T24878 1901-1914 VB denotes immunoblotted
T24879 1915-1918 IN denotes for
T24880 1919-1932 NN denotes phospho-NF-κB
T24881 1934-1937 DT denotes The
T24882 1938-1944 NN denotes purity
T24883 1945-1947 IN denotes of
T24884 1948-1951 DT denotes the
T24885 1952-1961 JJ denotes cytosolic
T24886 1962-1965 CC denotes and
T24887 1966-1973 JJ denotes nuclear
T24888 1974-1983 NN denotes fractions
T24889 1984-1987 VB denotes was
T24890 1988-1997 VB denotes confirmed
T24891 1998-2000 IN denotes by
T24892 2001-2015 VB denotes immunoblotting
T24893 2016-2020 IN denotes with
T24894 2021-2026 NN denotes GAPDH
T24895 2027-2030 CC denotes and
T24896 2031-2036 NN denotes Lamin
T24897 2037-2038 NN denotes B
T24898 2038-2039 -COMMA- denotes ,
T24899 2040-2052 RB denotes respectively
T24900 2054-2059 VB denotes Shown
T24901 2060-2063 VB denotes are
T24902 2064-2078 JJ denotes representative
T24903 2079-2084 NN denotes blots
T24904 2085-2089 IN denotes from
T24905 2090-2095 CD denotes three
T24906 2096-2107 JJ denotes independent
T24907 2108-2119 NN denotes experiments
R5668 T7623 T7622 arg1Of Activation,M-CSF-Dependent
R5669 T7623 T7624 arg1Of Activation,of
R5670 T7623 T7626 arg1Of Activation,Does
R5671 T7623 T7628 arg1Of Activation,Regulate
R5672 T7625 T7624 arg2Of PKC,of
R5673 T7628 T7626 arg2Of Regulate,Does
R5674 T7628 T7627 arg1Of Regulate,Not
R5675 T7634 T7628 arg2Of Pathway,Regulate
R5676 T7634 T7629 arg1Of Pathway,the
R5677 T7634 T7630 arg1Of Pathway,Classical
R5678 T7634 T7631 arg1Of Pathway,NF-κB
R5679 T7634 T7632 arg1Of Pathway,p65
R5680 T7634 T7633 arg1Of Pathway,Activation
R5681 T7636 T7635 arg1Of activation,Canonical
R5682 T7636 T7637 arg1Of activation,of
R5683 T7636 T7639 arg1Of activation,occurs
R5684 T7638 T7637 arg2Of NF-κB,of
R5685 T7639 T7640 arg1Of occurs,via
R5686 T7641 T7642 arg1Of phosphorylation,and
R5687 T7642 T7640 arg2Of and,via
R5688 T7642 T7644 arg1Of and,of
R5689 T7643 T7642 arg2Of degradation,and
R5690 T7645 T7644 arg2Of IκBα,of
R5691 T7645 T7646 arg1Of IκBα,leading
R5692 T7646 T7647 arg1Of leading,to
R5693 T7646 T7658 modOf leading,to
R5694 T7646 T7665 arg1Of leading,","
R5695 T7646 T7666 arg1Of leading,[
R5696 T7649 T7650 arg1Of release,and
R5697 T7650 T7647 arg2Of and,to
R5698 T7650 T7648 arg1Of and,the
R5699 T7650 T7653 arg1Of and,of
R5700 T7652 T7650 arg2Of translocation,and
R5701 T7652 T7651 arg1Of translocation,nuclear
R5702 T7657 T7653 arg2Of heterodimer,of
R5703 T7657 T7654 arg1Of heterodimer,the
R5704 T7657 T7655 arg1Of heterodimer,NF-κB
R5705 T7657 T7656 arg1Of heterodimer,p50/p65
R5706 T7659 T7658 arg1Of transactivate,to
R5707 T7661 T7659 arg2Of genes,transactivate
R5708 T7661 T7660 arg1Of genes,target
R5709 T7661 T7662 arg1Of genes,[
R5710 T7663 T7662 arg2Of 37,[
R5711 T7664 T7662 arg3Of ],[
R5712 T7667 T7666 arg2Of 39,[
R5713 T7668 T7666 arg3Of ],[
R5714 T7672 T7670 arg1Of activity,conventional
R5715 T7672 T7671 arg1Of activity,PKC
R5716 T7672 T7673 arg1Of activity,was
R5717 T7672 T7674 arg1Of activity,important
R5718 T7673 T7669 arg2Of was,Since
R5719 T7673 T7675 arg1Of was,in
R5720 T7674 T7673 arg2Of important,was
R5721 T7676 T7675 arg2Of regulating,in
R5722 T7679 T7676 arg2Of activation,regulating
R5723 T7679 T7677 arg1Of activation,M-CSF-induced
R5724 T7679 T7678 arg1Of activation,NF-κB
R5725 T7681 T7683 arg1Of we,investigated
R5726 T7683 T7669 arg1Of investigated,Since
R5727 T7683 T7680 arg1Of investigated,","
R5728 T7683 T7682 arg1Of investigated,next
R5729 T7686 T7685 arg1Of degradation,IκBα
R5730 T7686 T7687 arg1Of degradation,was
R5731 T7686 T7688 arg2Of degradation,regulated
R5732 T7688 T7683 arg2Of regulated,investigated
R5733 T7688 T7684 arg1Of regulated,whether
R5734 T7688 T7687 arg2Of regulated,was
R5735 T7690 T7688 arg1Of PKC,regulated
R5736 T7690 T7689 arg2Of PKC,by
R5737 T7691 T7692 arg1Of Cells,were
R5738 T7691 T7693 arg2Of Cells,treated
R5739 T7693 T7692 arg2Of treated,were
R5740 T7693 T7694 arg1Of treated,with
R5741 T7693 T7706 arg1Of treated,and
R5742 T7695 T7694 arg2Of cyclohexamide,with
R5743 T7695 T7696 arg1Of cyclohexamide,(
R5744 T7695 T7699 modOf cyclohexamide,to
R5745 T7697 T7696 arg2Of CHX,(
R5746 T7698 T7696 arg3Of ),(
R5747 T7700 T7699 arg1Of inhibit,to
R5748 T7702 T7700 arg2Of synthesis,inhibit
R5749 T7702 T7701 arg1Of synthesis,protein
R5750 T7702 T7703 arg1Of synthesis,of
R5751 T7704 T7703 arg2Of IκBα,of
R5752 T7706 T7705 arg1Of and,","
R5753 T7708 T7707 arg1Of degradation,its
R5754 T7708 T7709 arg1Of degradation,was
R5755 T7708 T7710 arg2Of degradation,followed
R5756 T7710 T7706 arg2Of followed,and
R5757 T7710 T7709 arg2Of followed,was
R5758 T7712 T7711 arg2Of shown,As
R5759 T7712 T7713 arg1Of shown,in
R5760 T7715 T7713 arg2Of 5A,in
R5761 T7715 T7714 arg1Of 5A,Figure
R5762 T7718 T7717 arg1Of inhibition,PKC
R5763 T7718 T7719 arg1Of inhibition,with
R5764 T7718 T7721 arg1Of inhibition,did
R5765 T7718 T7723 arg1Of inhibition,alter
R5766 T7720 T7719 arg2Of Ro-31-8220,with
R5767 T7723 T7711 arg1Of alter,As
R5768 T7723 T7716 arg1Of alter,","
R5769 T7723 T7721 arg2Of alter,did
R5770 T7723 T7722 arg1Of alter,not
R5771 T7723 T7727 arg1Of alter,","
R5772 T7723 T7728 modOf alter,suggesting
R5773 T7726 T7723 arg2Of degradation,alter
R5774 T7726 T7724 arg1Of degradation,M-CSF-induced
R5775 T7726 T7725 arg1Of degradation,IκBα
R5776 T7732 T7730 arg1Of activity,M-CSF-induced
R5777 T7732 T7731 arg1Of activity,PKC
R5778 T7732 T7733 arg1Of activity,augmented
R5779 T7733 T7728 arg2Of augmented,suggesting
R5780 T7733 T7729 arg1Of augmented,that
R5781 T7733 T7737 arg1Of augmented,by
R5782 T7733 T7741 arg1Of augmented,","
R5783 T7733 T7742 arg1Of augmented,like
R5784 T7736 T7733 arg2Of activity,augmented
R5785 T7736 T7734 arg1Of activity,NF-κB
R5786 T7736 T7735 arg1Of activity,transcriptional
R5787 T7740 T7737 arg2Of pathway,by
R5788 T7740 T7738 arg1Of pathway,an
R5789 T7740 T7739 arg1Of pathway,alternative
R5790 T7744 T7742 arg2Of modification,like
R5791 T7744 T7743 arg1Of modification,post-translational
R5792 T7744 T7745 arg1Of modification,of
R5793 T7747 T7745 arg2Of p65,of
R5794 T7747 T7746 arg1Of p65,NF-κB
R18880 T24716 T24714 arg1Of activity,M-CSF-induced
R18881 T24716 T24715 arg1Of activity,PKC
R18882 T24716 T24717 arg1Of activity,does
R18883 T24716 T24719 arg1Of activity,regulate
R18884 T24716 T24723 arg1Of activity,regulates
R18885 T24719 T24717 arg2Of regulate,does
R18886 T24719 T24718 arg1Of regulate,not
R18887 T24719 T24722 arg1Of regulate,but
R18888 T24721 T24719 arg2Of degradation,regulate
R18889 T24721 T24720 arg1Of degradation,IκBα
R18890 T24723 T24722 arg2Of regulates,but
R18891 T24725 T24723 arg2Of phosphorylation,regulates
R18892 T24725 T24724 arg1Of phosphorylation,the
R18893 T24725 T24726 arg1Of phosphorylation,of
R18894 T24728 T24726 arg2Of p65,of
R18895 T24728 T24727 arg1Of p65,NF-κB
R18896 T24728 T24729 arg1Of p65,at
R18897 T24730 T24729 arg2Of Ser276,at
R18898 T24732 T24731 arg2Of A,(
R18899 T24733 T24731 arg3Of ),(
R18900 T24734 T24731 arg1Of MDMs,(
R18901 T24734 T24735 arg1Of MDMs,were
R18902 T24734 T24736 arg2Of MDMs,pretreated
R18903 T24736 T24735 arg2Of pretreated,were
R18904 T24736 T24737 arg1Of pretreated,with
R18905 T24736 T24742 arg1Of pretreated,in
R18906 T24736 T24749 arg1Of pretreated,for
R18907 T24738 T24737 arg2Of cycloheximide,with
R18908 T24738 T24739 arg1Of cycloheximide,(
R18909 T24740 T24739 arg2Of CHX,(
R18910 T24741 T24739 arg3Of ),(
R18911 T24744 T24745 arg1Of absence,or
R18912 T24745 T24742 arg2Of or,in
R18913 T24745 T24743 arg1Of or,the
R18914 T24745 T24747 arg1Of or,of
R18915 T24746 T24745 arg2Of presence,or
R18916 T24748 T24747 arg2Of Ro-31-8220,of
R18917 T24751 T24749 arg2Of minutes,for
R18918 T24751 T24750 arg1Of minutes,30
R18919 T24751 T24753 arg1Of minutes,to
R18920 T24753 T24752 arg1Of to,prior
R18921 T24755 T24753 arg2Of stimulation,to
R18922 T24755 T24754 arg1Of stimulation,M-CSF
R18923 T24755 T24756 arg1Of stimulation,for
R18924 T24759 T24756 arg2Of times,for
R18925 T24759 T24757 arg1Of times,the
R18926 T24759 T24758 arg1Of times,indicated
R18927 T24762 T24760 arg1Of lysates,Whole
R18928 T24762 T24761 arg1Of lysates,cell
R18929 T24762 T24763 arg1Of lysates,were
R18930 T24762 T24764 arg2Of lysates,subjected
R18931 T24764 T24763 arg2Of subjected,were
R18932 T24764 T24765 arg1Of subjected,to
R18933 T24767 T24765 arg2Of blotting,to
R18934 T24767 T24766 arg1Of blotting,Western
R18935 T24767 T24768 arg1Of blotting,with
R18936 T24770 T24768 arg2Of antibody,with
R18937 T24770 T24769 arg1Of antibody,anti-IκBα
R18938 T24771 T24772 arg1Of Data,are
R18939 T24771 T24773 arg1Of Data,representative
R18940 T24773 T24772 arg2Of representative,are
R18941 T24773 T24774 arg1Of representative,of
R18942 T24777 T24774 arg2Of experiments.,of
R18943 T24777 T24775 arg1Of experiments.,three
R18944 T24777 T24776 arg1Of experiments.,independent
R18945 T24777 T24778 arg1Of experiments.,(
R18946 T24779 T24778 arg2Of B,(
R18947 T24780 T24778 arg3Of ),(
R18948 T24781 T24782 arg1Of MDMs,were
R18949 T24781 T24783 arg2Of MDMs,pretreated
R18950 T24783 T24773 arg2Of pretreated,representative
R18951 T24783 T24782 arg2Of pretreated,were
R18952 T24783 T24784 arg1Of pretreated,with
R18953 T24786 T24787 arg1Of vehicle,or
R18954 T24787 T24784 arg2Of or,with
R18955 T24787 T24785 arg1Of or,either
R18956 T24787 T24789 arg1Of or,for
R18957 T24788 T24787 arg2Of Ro-31-8220,or
R18958 T24791 T24789 arg2Of minutes,for
R18959 T24791 T24790 arg1Of minutes,30
R18960 T24791 T24792 arg1Of minutes,before
R18961 T24794 T24792 arg2Of addition,before
R18962 T24794 T24793 arg1Of addition,the
R18963 T24794 T24795 arg1Of addition,of
R18964 T24794 T24797 arg1Of addition,for
R18965 T24796 T24795 arg2Of M-CSF,of
R18966 T24799 T24797 arg2Of minutes,for
R18967 T24799 T24798 arg1Of minutes,10
R18968 T24802 T24800 arg1Of lysates,Whole
R18969 T24802 T24801 arg1Of lysates,cell
R18970 T24802 T24803 arg1Of lysates,were
R18971 T24802 T24804 arg2Of lysates,resolved
R18972 T24802 T24848 arg1Of lysates,were
R18973 T24802 T24849 arg2Of lysates,subjected
R18974 T24804 T24803 arg2Of resolved,were
R18975 T24804 T24805 arg1Of resolved,by
R18976 T24804 T24807 arg1Of resolved,and
R18977 T24806 T24805 arg2Of SDS-PAGE,by
R18978 T24808 T24809 arg1Of phospho-Ser276,or
R18979 T24809 T24811 arg1Of or,of
R18980 T24809 T24814 arg1Of or,was
R18981 T24809 T24815 arg2Of or,detected
R18982 T24809 T24816 arg1Of or,using
R18983 T24810 T24809 arg2Of phospho-Ser536,or
R18984 T24813 T24811 arg2Of p65,of
R18985 T24813 T24812 arg1Of p65,NF-κB
R18986 T24815 T24814 arg2Of detected,was
R18987 T24816 T24815 arg3Of using,detected
R18988 T24818 T24816 arg2Of antibodies,using
R18989 T24818 T24817 arg1Of antibodies,phospho-specific
R18990 T24818 T24819 arg1Of antibodies,to
R18991 T24821 T24819 arg2Of residue,to
R18992 T24821 T24820 arg1Of residue,either
R18993 T24821 T24822 arg1Of residue,of
R18994 T24826 T24825 arg2Of C,(
R18995 T24827 T24825 arg3Of ),(
R18996 T24830 T24822 arg2Of lysates,of
R18997 T24830 T24823 arg1Of lysates,NF-κB
R18998 T24830 T24824 arg1Of lysates,p65.
R18999 T24830 T24825 arg1Of lysates,(
R19000 T24830 T24828 arg1Of lysates,Whole
R19001 T24830 T24829 arg1Of lysates,cell
R19002 T24830 T24831 arg1Of lysates,of
R19003 T24834 T24831 arg2Of cells,of
R19004 T24834 T24832 arg1Of cells,RAW
R19005 T24834 T24833 arg1Of cells,264.7
R19006 T24834 T24835 arg2Of cells,treated
R19007 T24835 T24836 arg1Of treated,with
R19008 T24835 T24841 arg1Of treated,in
R19009 T24838 T24839 arg1Of control,or
R19010 T24839 T24836 arg2Of or,with
R19011 T24839 T24837 arg1Of or,vehicle
R19012 T24840 T24839 arg2Of Ro-31-8220,or
R19013 T24843 T24844 arg1Of absence,or
R19014 T24844 T24841 arg2Of or,in
R19015 T24844 T24842 arg1Of or,the
R19016 T24844 T24846 arg1Of or,of
R19017 T24845 T24844 arg2Of presence,or
R19018 T24847 T24846 arg2Of M-CSF,of
R19019 T24849 T24807 arg2Of subjected,and
R19020 T24849 T24814 modOf subjected,was
R19021 T24849 T24848 arg2Of subjected,were
R19022 T24849 T24850 arg1Of subjected,to
R19023 T24853 T24851 arg1Of analysis,Western
R19024 T24853 T24852 arg1Of analysis,blot
R19025 T24853 T24854 arg1Of analysis,with
R19026 T24853 T24856 arg1Of analysis,or
R19027 T24855 T24854 arg2Of phospho-Ser276,with
R19028 T24856 T24850 arg2Of or,to
R19029 T24856 T24871 modOf or,were
R19030 T24860 T24856 arg2Of antibodies.,or
R19031 T24860 T24857 arg1Of antibodies.,phospho-Ser536
R19032 T24860 T24858 arg1Of antibodies.,NF-κB
R19033 T24860 T24859 arg1Of antibodies.,p65
R19034 T24860 T24861 arg1Of antibodies.,(
R19035 T24862 T24861 arg2Of D,(
R19036 T24863 T24861 arg3Of ),(
R19037 T24864 T24865 arg1Of Cytosolic,and
R19038 T24866 T24865 arg2Of nuclear,and
R19039 T24867 T24864 arg1Of fractions,Cytosolic
R19040 T24867 T24866 arg1Of fractions,nuclear
R19041 T24867 T24868 arg1Of fractions,of
R19042 T24867 T24871 arg1Of fractions,were
R19043 T24867 T24872 arg2Of fractions,obtained
R19044 T24867 T24878 arg2Of fractions,immunoblotted
R19045 T24869 T24868 arg2Of RAW,of
R19046 T24869 T24870 arg1Of RAW,264.7
R19047 T24872 T24873 arg1Of obtained,from
R19048 T24872 T24877 arg1Of obtained,and
R19049 T24876 T24873 arg2Of cells,from
R19050 T24876 T24874 arg1Of cells,the
R19051 T24876 T24875 arg2Of cells,treated
R19052 T24877 T24871 arg2Of and,were
R19053 T24878 T24877 arg2Of immunoblotted,and
R19054 T24878 T24879 arg1Of immunoblotted,for
R19055 T24880 T24879 arg2Of phospho-NF-κB,for
R19056 T24882 T24881 arg1Of purity,The
R19057 T24882 T24883 arg1Of purity,of
R19058 T24882 T24889 arg1Of purity,was
R19059 T24882 T24890 arg2Of purity,confirmed
R19060 T24885 T24886 arg1Of cytosolic,and
R19061 T24887 T24886 arg2Of nuclear,and
R19062 T24888 T24883 arg2Of fractions,of
R19063 T24888 T24884 arg1Of fractions,the
R19064 T24888 T24885 arg1Of fractions,cytosolic
R19065 T24888 T24887 arg1Of fractions,nuclear
R19066 T24890 T24889 arg2Of confirmed,was
R19067 T24890 T24891 arg1Of confirmed,by
R19068 T24890 T24898 arg1Of confirmed,","
R19069 T24890 T24899 arg1Of confirmed,respectively
R19070 T24892 T24891 arg2Of immunoblotting,by
R19071 T24892 T24893 arg1Of immunoblotting,with
R19072 T24894 T24895 arg1Of GAPDH,and
R19073 T24895 T24893 arg2Of and,with
R19074 T24897 T24895 arg2Of B,and
R19075 T24897 T24896 arg1Of B,Lamin
R19076 T24901 T24900 modOf are,Shown
R19077 T24903 T24900 arg2Of blots,Shown
R19078 T24903 T24901 arg1Of blots,are
R19079 T24903 T24902 arg1Of blots,representative
R19080 T24903 T24904 arg1Of blots,from
R19081 T24907 T24904 arg2Of experiments,from
R19082 T24907 T24905 arg1Of experiments,three
R19083 T24907 T24906 arg1Of experiments,independent

sentences

Id Subject Object Predicate Lexical cue
T7487 0-94 Sentence denotes M-CSF-Dependent Activation of PKC Does Not Regulate the Classical NF-κB p65 Activation Pathway
T7488 95-299 Sentence denotes Canonical activation of NF-κB occurs via phosphorylation and degradation of IκBα leading to the release and nuclear translocation of the NF-κB p50/p65 heterodimer to transactivate target genes [37], [39].
T7489 300-459 Sentence denotes Since conventional PKC activity was important in regulating M-CSF-induced NF-κB activation, we next investigated whether IκBα degradation was regulated by PKC.
T7490 460-575 Sentence denotes Cells were treated with cyclohexamide (CHX) to inhibit protein synthesis of IκBα, and its degradation was followed.
T7491 576-837 Sentence denotes As shown in Figure 5A, PKC inhibition with Ro-31-8220 did not alter M-CSF-induced IκBα degradation, suggesting that M-CSF-induced PKC activity augmented NF-κB transcriptional activity by an alternative pathway, like post-translational modification of NF-κB p65.
T24503 882-1001 Sentence denotes M-CSF-induced PKC activity does not regulate IκBα degradation but regulates the phosphorylation of NF-κB p65 at Ser276.
T24504 1002-1159 Sentence denotes (A) MDMs were pretreated with cycloheximide (CHX) in the absence or presence of Ro-31-8220 for 30 minutes prior to M-CSF stimulation for the indicated times.
T24505 1160-1238 Sentence denotes Whole cell lysates were subjected to Western blotting with anti-IκBα antibody.
T24506 1239-1415 Sentence denotes Data are representative of three independent experiments. (B) MDMs were pretreated with either vehicle or Ro-31-8220 for 30 minutes before the addition of M-CSF for 10 minutes.
T24507 1416-1933 Sentence denotes Whole cell lysates were resolved by SDS-PAGE and phospho-Ser276 or phospho-Ser536 of NF-κB p65 was detected using phospho-specific antibodies to either residue of NF-κB p65. (C) Whole cell lysates of RAW 264.7 cells treated with vehicle control or Ro-31-8220 in the absence or presence of M-CSF were subjected to Western blot analysis with phospho-Ser276 or phospho-Ser536 NF-κB p65 antibodies. (D) Cytosolic and nuclear fractions of RAW 264.7 were obtained from the treated cells and immunoblotted for phospho-NF-κB.
T24508 1934-2053 Sentence denotes The purity of the cytosolic and nuclear fractions was confirmed by immunoblotting with GAPDH and Lamin B, respectively.
T24509 2054-2120 Sentence denotes Shown are representative blots from three independent experiments.
T128 0-94 Sentence denotes M-CSF-Dependent Activation of PKC Does Not Regulate the Classical NF-κB p65 Activation Pathway
T129 95-299 Sentence denotes Canonical activation of NF-κB occurs via phosphorylation and degradation of IκBα leading to the release and nuclear translocation of the NF-κB p50/p65 heterodimer to transactivate target genes [37], [39].
T130 300-459 Sentence denotes Since conventional PKC activity was important in regulating M-CSF-induced NF-κB activation, we next investigated whether IκBα degradation was regulated by PKC.
T131 460-575 Sentence denotes Cells were treated with cyclohexamide (CHX) to inhibit protein synthesis of IκBα, and its degradation was followed.
T132 576-837 Sentence denotes As shown in Figure 5A, PKC inhibition with Ro-31-8220 did not alter M-CSF-induced IκBα degradation, suggesting that M-CSF-induced PKC activity augmented NF-κB transcriptional activity by an alternative pathway, like post-translational modification of NF-κB p65.
T133 838-1001 Sentence denotes 10.1371/journal.pone.0028081.g005 Figure 5 M-CSF-induced PKC activity does not regulate IκBα degradation but regulates the phosphorylation of NF-κB p65 at Ser276.
T134 1002-1159 Sentence denotes (A) MDMs were pretreated with cycloheximide (CHX) in the absence or presence of Ro-31-8220 for 30 minutes prior to M-CSF stimulation for the indicated times.
T135 1160-1238 Sentence denotes Whole cell lysates were subjected to Western blotting with anti-IκBα antibody.
T136 1239-1415 Sentence denotes Data are representative of three independent experiments. (B) MDMs were pretreated with either vehicle or Ro-31-8220 for 30 minutes before the addition of M-CSF for 10 minutes.
T137 1416-1933 Sentence denotes Whole cell lysates were resolved by SDS-PAGE and phospho-Ser276 or phospho-Ser536 of NF-κB p65 was detected using phospho-specific antibodies to either residue of NF-κB p65. (C) Whole cell lysates of RAW 264.7 cells treated with vehicle control or Ro-31-8220 in the absence or presence of M-CSF were subjected to Western blot analysis with phospho-Ser276 or phospho-Ser536 NF-κB p65 antibodies. (D) Cytosolic and nuclear fractions of RAW 264.7 were obtained from the treated cells and immunoblotted for phospho-NF-κB.
T138 1934-2053 Sentence denotes The purity of the cytosolic and nuclear fractions was confirmed by immunoblotting with GAPDH and Lamin B, respectively.
T139 2054-2120 Sentence denotes Shown are representative blots from three independent experiments.

2_test

Id Subject Object Predicate Lexical cue
22216091-12861375-90609713 289-291 12861375 denotes 37
22216091-9346241-90609714 295-297 9346241 denotes 39

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T7492 0-15 JJ denotes M-CSF-Dependent
T7493 16-26 NNP denotes Activation
T7494 27-29 IN denotes of
T7495 30-33 NNP denotes PKC
T7496 34-38 VBZ denotes Does
T7497 39-42 RB denotes Not
T7498 43-51 VB denotes Regulate
T7499 52-55 DT denotes the
T7500 56-65 JJ denotes Classical
T7501 66-71 NN denotes NF-κB
T7502 72-75 CD denotes p65
T7503 76-86 NNP denotes Activation
T7504 87-94 NNP denotes Pathway
T7505 95-104 NNP denotes Canonical
T7506 105-115 NN denotes activation
T7507 116-118 IN denotes of
T7508 119-124 JJ denotes NF-κB
T7509 125-131 VBZ denotes occurs
T7510 132-135 IN denotes via
T7511 136-151 NN denotes phosphorylation
T7512 152-155 CC denotes and
T7513 156-167 NN denotes degradation
T7514 168-170 IN denotes of
T7515 171-175 NNP denotes IκBα
T7516 176-183 VBG denotes leading
T7517 184-186 TO denotes to
T7518 187-190 DT denotes the
T7519 191-198 NN denotes release
T7520 199-202 CC denotes and
T7521 203-210 JJ denotes nuclear
T7522 211-224 NN denotes translocation
T7523 225-227 IN denotes of
T7524 228-231 DT denotes the
T7525 232-237 NNP denotes NF-κB
T7526 238-245 CD denotes p50/p65
T7527 246-257 NN denotes heterodimer
T7528 258-260 TO denotes to
T7529 261-274 VB denotes transactivate
T7530 275-281 NN denotes target
T7531 282-287 NNS denotes genes
T7532 288-289 NNP denotes [
T7533 289-291 CD denotes 37
T7534 291-292 NNP denotes ]
T7535 292-293 , denotes ,
T7536 294-295 NNP denotes [
T7537 295-297 CD denotes 39
T7538 297-298 NNP denotes ]
T7539 298-299 . denotes .
T7540 300-305 IN denotes Since
T7541 306-318 JJ denotes conventional
T7542 319-322 NNP denotes PKC
T7543 323-331 NN denotes activity
T7544 332-335 VBD denotes was
T7545 336-345 JJ denotes important
T7546 346-348 IN denotes in
T7547 349-359 VBG denotes regulating
T7548 360-373 JJ denotes M-CSF-induced
T7549 374-379 NN denotes NF-κB
T7550 380-390 NN denotes activation
T7551 390-391 , denotes ,
T7552 392-394 PRP denotes we
T7553 395-399 JJ denotes next
T7554 400-412 VBD denotes investigated
T7555 413-420 IN denotes whether
T7556 421-425 NNP denotes IκBα
T7557 426-437 NN denotes degradation
T7558 438-441 VBD denotes was
T7559 442-451 VBN denotes regulated
T7560 452-454 IN denotes by
T7561 455-458 NNP denotes PKC
T7562 458-459 . denotes .
T7563 460-465 NNS denotes Cells
T7564 466-470 VBD denotes were
T7565 471-478 VBN denotes treated
T7566 479-483 IN denotes with
T7567 484-497 NN denotes cyclohexamide
T7568 498-499 -LRB- denotes (
T7569 499-502 NNP denotes CHX
T7570 502-503 -RRB- denotes )
T7571 504-506 TO denotes to
T7572 507-514 VB denotes inhibit
T7573 515-522 NN denotes protein
T7574 523-532 NN denotes synthesis
T7575 533-535 IN denotes of
T7576 536-540 NNP denotes IκBα
T7577 540-541 , denotes ,
T7578 542-545 CC denotes and
T7579 546-549 PRP$ denotes its
T7580 550-561 NN denotes degradation
T7581 562-565 VBD denotes was
T7582 566-574 VBN denotes followed
T7583 574-575 . denotes .
T7584 576-578 IN denotes As
T7585 579-584 VBN denotes shown
T7586 585-587 IN denotes in
T7587 588-594 NN denotes Figure
T7588 595-597 CD denotes 5A
T7589 597-598 , denotes ,
T7590 599-602 NNP denotes PKC
T7591 603-613 NN denotes inhibition
T7592 614-618 IN denotes with
T7593 619-629 NN denotes Ro-31-8220
T7594 630-633 VBD denotes did
T7595 634-637 RB denotes not
T7596 638-643 VB denotes alter
T7597 644-657 JJ denotes M-CSF-induced
T7598 658-662 NNP denotes IκBα
T7599 663-674 NN denotes degradation
T7600 674-675 , denotes ,
T7601 676-686 VBG denotes suggesting
T7602 687-691 IN denotes that
T7603 692-705 JJ denotes M-CSF-induced
T7604 706-709 NNP denotes PKC
T7605 710-718 NN denotes activity
T7606 719-728 VBD denotes augmented
T7607 729-734 JJ denotes NF-κB
T7608 735-750 JJ denotes transcriptional
T7609 751-759 NN denotes activity
T7610 760-762 IN denotes by
T7611 763-765 DT denotes an
T7612 766-777 JJ denotes alternative
T7613 778-785 NN denotes pathway
T7614 785-786 , denotes ,
T7615 787-791 IN denotes like
T7616 792-810 JJ denotes post-translational
T7617 811-823 NN denotes modification
T7618 824-826 IN denotes of
T7619 827-832 NNP denotes NF-κB
T7620 833-836 NNS denotes p65
T7621 836-837 . denotes .
T24550 1114-1116 TO denotes to
T24510 882-895 JJ denotes M-CSF-induced
T24511 896-899 NN denotes PKC
T24512 900-908 NN denotes activity
T24513 909-913 VBZ denotes does
T24514 914-917 RB denotes not
T24515 918-926 VB denotes regulate
T24516 927-931 NNP denotes IκBα
T24517 932-943 NN denotes degradation
T24518 944-947 CC denotes but
T24519 948-957 VBZ denotes regulates
T24520 958-961 DT denotes the
T24521 962-977 NN denotes phosphorylation
T24522 978-980 IN denotes of
T24523 981-986 NNP denotes NF-κB
T24524 987-990 NNS denotes p65
T24525 991-993 IN denotes at
T24526 994-1000 NNP denotes Ser276
T24527 1000-1001 . denotes .
T24528 1002-1003 -LRB- denotes (
T24529 1003-1004 NNP denotes A
T24530 1004-1005 -RRB- denotes )
T24531 1006-1010 NNS denotes MDMs
T24532 1011-1015 VBD denotes were
T24533 1016-1026 VBN denotes pretreated
T24534 1027-1031 IN denotes with
T24535 1032-1045 NN denotes cycloheximide
T24536 1046-1047 -LRB- denotes (
T24537 1047-1050 NNP denotes CHX
T24538 1050-1051 -RRB- denotes )
T24539 1052-1054 IN denotes in
T24540 1055-1058 DT denotes the
T24541 1059-1066 NN denotes absence
T24542 1067-1069 CC denotes or
T24543 1070-1078 NN denotes presence
T24544 1079-1081 IN denotes of
T24545 1082-1092 NN denotes Ro-31-8220
T24546 1093-1096 IN denotes for
T24547 1097-1099 CD denotes 30
T24548 1100-1107 NNS denotes minutes
T24549 1108-1113 RB denotes prior
T24551 1117-1122 NNP denotes M-CSF
T24552 1123-1134 NN denotes stimulation
T24553 1135-1138 IN denotes for
T24554 1139-1142 DT denotes the
T24555 1143-1152 JJ denotes indicated
T24556 1153-1158 NNS denotes times
T24557 1158-1159 . denotes .
T24558 1160-1165 JJ denotes Whole
T24559 1166-1170 NN denotes cell
T24560 1171-1178 NNS denotes lysates
T24561 1179-1183 VBD denotes were
T24562 1184-1193 VBN denotes subjected
T24563 1194-1196 TO denotes to
T24564 1197-1204 JJ denotes Western
T24565 1205-1213 VBG denotes blotting
T24566 1214-1218 IN denotes with
T24567 1219-1228 JJ denotes anti-IκBα
T24568 1229-1237 NN denotes antibody
T24569 1237-1238 . denotes .
T24570 1239-1243 NNS denotes Data
T24571 1244-1247 VBP denotes are
T24572 1248-1262 NN denotes representative
T24573 1263-1265 IN denotes of
T24574 1266-1271 CD denotes three
T24575 1272-1283 JJ denotes independent
T24576 1284-1295 NNS denotes experiments
T24577 1295-1296 . denotes .
T24578 1297-1298 -LRB- denotes (
T24579 1298-1299 NNP denotes B
T24580 1299-1300 -RRB- denotes )
T24581 1301-1305 NNS denotes MDMs
T24582 1306-1310 VBD denotes were
T24583 1311-1321 VBN denotes pretreated
T24584 1322-1326 IN denotes with
T24585 1327-1333 DT denotes either
T24586 1334-1341 NN denotes vehicle
T24587 1342-1344 CC denotes or
T24588 1345-1355 NN denotes Ro-31-8220
T24589 1356-1359 IN denotes for
T24590 1360-1362 CD denotes 30
T24591 1363-1370 NNS denotes minutes
T24592 1371-1377 IN denotes before
T24593 1378-1381 DT denotes the
T24594 1382-1390 NN denotes addition
T24595 1391-1393 IN denotes of
T24596 1394-1399 NNP denotes M-CSF
T24597 1400-1403 IN denotes for
T24598 1404-1406 CD denotes 10
T24599 1407-1414 NNS denotes minutes
T24600 1414-1415 . denotes .
T24601 1416-1421 JJ denotes Whole
T24602 1422-1426 NN denotes cell
T24603 1427-1434 NNS denotes lysates
T24604 1435-1439 VBD denotes were
T24605 1440-1448 VBN denotes resolved
T24606 1449-1451 IN denotes by
T24607 1452-1460 NNP denotes SDS-PAGE
T24608 1461-1464 CC denotes and
T24609 1465-1479 NNP denotes phospho-Ser276
T24610 1480-1482 CC denotes or
T24611 1483-1497 NNP denotes phospho-Ser536
T24612 1498-1500 IN denotes of
T24613 1501-1506 NNP denotes NF-κB
T24614 1507-1510 CD denotes p65
T24615 1511-1514 VBD denotes was
T24616 1515-1523 VBN denotes detected
T24617 1524-1529 VBG denotes using
T24618 1530-1546 JJ denotes phospho-specific
T24619 1547-1557 NNS denotes antibodies
T24620 1558-1560 TO denotes to
T24621 1561-1567 DT denotes either
T24622 1568-1575 NN denotes residue
T24623 1576-1578 IN denotes of
T24624 1579-1584 NNP denotes NF-κB
T24625 1585-1588 NNS denotes p65
T24626 1588-1589 . denotes .
T24627 1590-1591 -LRB- denotes (
T24628 1591-1592 $ denotes C
T24629 1592-1593 -RRB- denotes )
T24630 1594-1599 JJ denotes Whole
T24631 1600-1604 NN denotes cell
T24632 1605-1612 NNS denotes lysates
T24633 1613-1615 IN denotes of
T24634 1616-1619 NNP denotes RAW
T24635 1620-1625 CD denotes 264.7
T24636 1626-1631 NNS denotes cells
T24637 1632-1639 VBN denotes treated
T24638 1640-1644 IN denotes with
T24639 1645-1652 NN denotes vehicle
T24640 1653-1660 NN denotes control
T24641 1661-1663 CC denotes or
T24642 1664-1674 NN denotes Ro-31-8220
T24643 1675-1677 IN denotes in
T24644 1678-1681 DT denotes the
T24645 1682-1689 NN denotes absence
T24646 1690-1692 CC denotes or
T24647 1693-1701 NN denotes presence
T24648 1702-1704 IN denotes of
T24649 1705-1710 NNP denotes M-CSF
T24650 1711-1715 VBD denotes were
T24651 1716-1725 VBN denotes subjected
T24652 1726-1728 TO denotes to
T24653 1729-1736 JJ denotes Western
T24654 1737-1741 NN denotes blot
T24655 1742-1750 NN denotes analysis
T24656 1751-1755 IN denotes with
T24657 1756-1770 NN denotes phospho-Ser276
T24658 1771-1773 CC denotes or
T24659 1774-1788 NN denotes phospho-Ser536
T24660 1789-1794 NNP denotes NF-κB
T24661 1795-1798 CD denotes p65
T24662 1799-1809 NNS denotes antibodies
T24663 1809-1810 . denotes .
T24664 1811-1812 -LRB- denotes (
T24665 1812-1813 NNP denotes D
T24666 1813-1814 -RRB- denotes )
T24667 1815-1824 NNP denotes Cytosolic
T24668 1825-1828 CC denotes and
T24669 1829-1836 JJ denotes nuclear
T24670 1837-1846 NNS denotes fractions
T24671 1847-1849 IN denotes of
T24672 1850-1853 NNP denotes RAW
T24673 1854-1859 CD denotes 264.7
T24674 1860-1864 VBD denotes were
T24675 1865-1873 VBN denotes obtained
T24676 1874-1878 IN denotes from
T24677 1879-1882 DT denotes the
T24678 1883-1890 VBN denotes treated
T24679 1891-1896 NNS denotes cells
T24680 1897-1900 CC denotes and
T24681 1901-1914 VBN denotes immunoblotted
T24682 1915-1918 IN denotes for
T24683 1919-1932 NN denotes phospho-NF-κB
T24684 1932-1933 . denotes .
T24685 1934-1937 DT denotes The
T24686 1938-1944 NN denotes purity
T24687 1945-1947 IN denotes of
T24688 1948-1951 DT denotes the
T24689 1952-1961 JJ denotes cytosolic
T24690 1962-1965 CC denotes and
T24691 1966-1973 JJ denotes nuclear
T24692 1974-1983 NNS denotes fractions
T24693 1984-1987 VBD denotes was
T24694 1988-1997 VBN denotes confirmed
T24695 1998-2000 IN denotes by
T24696 2001-2015 VBG denotes immunoblotting
T24697 2016-2020 IN denotes with
T24698 2021-2026 NNP denotes GAPDH
T24699 2027-2030 CC denotes and
T24700 2031-2036 NNP denotes Lamin
T24701 2037-2038 NNP denotes B
T24702 2038-2039 , denotes ,
T24703 2040-2052 RB denotes respectively
T24704 2052-2053 . denotes .
T24705 2054-2059 VBN denotes Shown
T24706 2060-2063 VBP denotes are
T24707 2064-2078 JJ denotes representative
T24708 2079-2084 NNS denotes blots
T24709 2085-2089 IN denotes from
T24710 2090-2095 CD denotes three
T24711 2096-2107 JJ denotes independent
T24712 2108-2119 NNS denotes experiments
T24713 2119-2120 . denotes .
R5538 T7492 T7493 amod M-CSF-Dependent,Activation
R5539 T7493 T7498 nsubj Activation,Regulate
R5540 T7494 T7493 prep of,Activation
R5541 T7495 T7494 pobj PKC,of
R5542 T7496 T7498 aux Does,Regulate
R5543 T7497 T7498 neg Not,Regulate
R5544 T7498 T7498 ROOT Regulate,Regulate
R5545 T7499 T7506 det the,activation
R5546 T7500 T7501 amod Classical,NF-κB
R5547 T7501 T7506 nmod NF-κB,activation
R5548 T7502 T7506 nummod p65,activation
R5549 T7503 T7504 compound Activation,Pathway
R5550 T7504 T7506 nmod Pathway,activation
R5551 T7505 T7506 amod Canonical,activation
R5552 T7506 T7509 nsubj activation,occurs
R5553 T7507 T7506 prep of,activation
R5554 T7508 T7507 pobj NF-κB,of
R5555 T7509 T7498 ccomp occurs,Regulate
R5556 T7510 T7509 prep via,occurs
R5557 T7511 T7510 pobj phosphorylation,via
R5558 T7512 T7511 cc and,phosphorylation
R5559 T7513 T7511 conj degradation,phosphorylation
R5560 T7514 T7513 prep of,degradation
R5561 T7515 T7516 nsubj IκBα,leading
R5562 T7516 T7498 advcl leading,Regulate
R5563 T7517 T7516 prep to,leading
R5564 T7518 T7519 det the,release
R5565 T7519 T7517 pobj release,to
R5566 T7520 T7519 cc and,release
R5567 T7521 T7522 amod nuclear,translocation
R5568 T7522 T7519 conj translocation,release
R5569 T7523 T7522 prep of,translocation
R5570 T7524 T7527 det the,heterodimer
R5571 T7525 T7527 nmod NF-κB,heterodimer
R5572 T7526 T7527 nummod p50/p65,heterodimer
R5573 T7527 T7523 pobj heterodimer,of
R5574 T7528 T7529 aux to,transactivate
R5575 T7529 T7516 xcomp transactivate,leading
R5576 T7530 T7531 compound target,genes
R5577 T7531 T7529 dobj genes,transactivate
R5578 T7532 T7534 nmod [,]
R5579 T7533 T7534 nummod 37,]
R5580 T7534 T7529 conj ],transactivate
R5581 T7535 T7534 punct ",",]
R5582 T7536 T7538 nmod [,]
R5583 T7537 T7538 nummod 39,]
R5584 T7538 T7534 appos ],]
R5585 T7539 T7498 punct .,Regulate
R5586 T7540 T7544 mark Since,was
R5587 T7541 T7543 amod conventional,activity
R5588 T7542 T7543 compound PKC,activity
R5589 T7543 T7544 nsubj activity,was
R5590 T7544 T7554 advcl was,investigated
R5591 T7545 T7544 acomp important,was
R5592 T7546 T7545 prep in,important
R5593 T7547 T7546 pcomp regulating,in
R5594 T7548 T7550 amod M-CSF-induced,activation
R5595 T7549 T7550 compound NF-κB,activation
R5596 T7550 T7547 dobj activation,regulating
R5597 T7551 T7554 punct ",",investigated
R5598 T7552 T7554 nsubj we,investigated
R5599 T7553 T7554 advmod next,investigated
R5600 T7554 T7554 ROOT investigated,investigated
R5601 T7555 T7559 mark whether,regulated
R5602 T7556 T7557 compound IκBα,degradation
R5603 T7557 T7559 nsubjpass degradation,regulated
R5604 T7558 T7559 auxpass was,regulated
R5605 T7559 T7554 ccomp regulated,investigated
R5606 T7560 T7559 agent by,regulated
R5607 T7561 T7560 pobj PKC,by
R5608 T7562 T7554 punct .,investigated
R5609 T7563 T7565 nsubjpass Cells,treated
R5610 T7564 T7565 auxpass were,treated
R5611 T7565 T7565 ROOT treated,treated
R5612 T7566 T7565 prep with,treated
R5613 T7567 T7566 pobj cyclohexamide,with
R5614 T7568 T7567 punct (,cyclohexamide
R5615 T7569 T7567 appos CHX,cyclohexamide
R5616 T7570 T7567 punct ),cyclohexamide
R5617 T7571 T7572 aux to,inhibit
R5618 T7572 T7565 advcl inhibit,treated
R5619 T7573 T7574 compound protein,synthesis
R5620 T7574 T7572 dobj synthesis,inhibit
R5621 T7575 T7574 prep of,synthesis
R5622 T7576 T7575 pobj IκBα,of
R5623 T7577 T7572 punct ",",inhibit
R5624 T7578 T7572 cc and,inhibit
R5625 T7579 T7580 poss its,degradation
R5626 T7580 T7582 nsubjpass degradation,followed
R5627 T7581 T7582 auxpass was,followed
R5628 T7582 T7565 conj followed,treated
R5629 T7583 T7582 punct .,followed
R5630 T7584 T7585 mark As,shown
R5631 T7585 T7596 advcl shown,alter
R5632 T7586 T7585 prep in,shown
R5633 T7587 T7586 pobj Figure,in
R5634 T7588 T7587 nummod 5A,Figure
R5635 T7589 T7585 punct ",",shown
R5636 T7590 T7591 compound PKC,inhibition
R5637 T7591 T7596 nsubj inhibition,alter
R5638 T7592 T7591 prep with,inhibition
R5639 T7593 T7592 pobj Ro-31-8220,with
R5640 T7594 T7596 aux did,alter
R5641 T7595 T7596 neg not,alter
R5642 T7596 T7596 ROOT alter,alter
R5643 T7597 T7599 amod M-CSF-induced,degradation
R5644 T7598 T7599 compound IκBα,degradation
R5645 T7599 T7596 dobj degradation,alter
R5646 T7600 T7596 punct ",",alter
R5647 T7601 T7596 advcl suggesting,alter
R5648 T7602 T7606 mark that,augmented
R5649 T7603 T7605 amod M-CSF-induced,activity
R5650 T7604 T7605 compound PKC,activity
R5651 T7605 T7606 nsubj activity,augmented
R5652 T7606 T7601 ccomp augmented,suggesting
R5653 T7607 T7609 amod NF-κB,activity
R5654 T7608 T7609 amod transcriptional,activity
R5655 T7609 T7606 dobj activity,augmented
R5656 T7610 T7609 prep by,activity
R5657 T7611 T7613 det an,pathway
R5658 T7612 T7613 amod alternative,pathway
R5659 T7613 T7610 pobj pathway,by
R5660 T7614 T7613 punct ",",pathway
R5661 T7615 T7606 prep like,augmented
R5662 T7616 T7617 amod post-translational,modification
R5663 T7617 T7615 pobj modification,like
R5664 T7618 T7617 prep of,modification
R5665 T7619 T7620 compound NF-κB,p65
R5666 T7620 T7618 pobj p65,of
R5667 T7621 T7596 punct .,alter
R18676 T24510 T24512 amod M-CSF-induced,activity
R18677 T24511 T24512 compound PKC,activity
R18678 T24512 T24515 nsubj activity,regulate
R18679 T24513 T24515 aux does,regulate
R18680 T24514 T24515 neg not,regulate
R18681 T24515 T24515 ROOT regulate,regulate
R18682 T24516 T24517 compound IκBα,degradation
R18683 T24517 T24515 dobj degradation,regulate
R18684 T24518 T24515 cc but,regulate
R18685 T24519 T24515 conj regulates,regulate
R18686 T24520 T24521 det the,phosphorylation
R18687 T24521 T24519 dobj phosphorylation,regulates
R18688 T24522 T24521 prep of,phosphorylation
R18689 T24523 T24524 compound NF-κB,p65
R18690 T24524 T24522 pobj p65,of
R18691 T24525 T24519 prep at,regulates
R18692 T24526 T24525 pobj Ser276,at
R18693 T24527 T24515 punct .,regulate
R18694 T24528 T24529 punct (,A
R18695 T24529 T24529 ROOT A,A
R18696 T24530 T24529 punct ),A
R18697 T24531 T24533 nsubjpass MDMs,pretreated
R18698 T24532 T24533 auxpass were,pretreated
R18699 T24533 T24533 ROOT pretreated,pretreated
R18700 T24534 T24533 prep with,pretreated
R18701 T24535 T24534 pobj cycloheximide,with
R18702 T24536 T24535 punct (,cycloheximide
R18703 T24537 T24535 appos CHX,cycloheximide
R18704 T24538 T24535 punct ),cycloheximide
R18713 T24547 T24548 nummod 30,minutes
R18714 T24548 T24546 pobj minutes,for
R18715 T24549 T24533 advmod prior,pretreated
R18716 T24550 T24549 prep to,prior
R18717 T24551 T24552 compound M-CSF,stimulation
R18718 T24552 T24550 pobj stimulation,to
R18719 T24553 T24549 prep for,prior
R18720 T24554 T24556 det the,times
R18721 T24555 T24556 amod indicated,times
R18722 T24556 T24553 pobj times,for
R18723 T24557 T24533 punct .,pretreated
R18724 T24558 T24560 amod Whole,lysates
R18725 T24559 T24560 compound cell,lysates
R18726 T24560 T24562 nsubjpass lysates,subjected
R18727 T24561 T24562 auxpass were,subjected
R18728 T24562 T24562 ROOT subjected,subjected
R18729 T24563 T24562 prep to,subjected
R18730 T24564 T24565 amod Western,blotting
R18731 T24565 T24563 pobj blotting,to
R18732 T24566 T24562 prep with,subjected
R18733 T24567 T24568 amod anti-IκBα,antibody
R18734 T24568 T24566 pobj antibody,with
R18735 T24569 T24562 punct .,subjected
R18736 T24570 T24571 nsubj Data,are
R18737 T24571 T24571 ROOT are,are
R18738 T24572 T24571 attr representative,are
R18739 T24573 T24572 prep of,representative
R18740 T24574 T24576 nummod three,experiments
R18741 T24575 T24576 amod independent,experiments
R18742 T24576 T24573 pobj experiments,of
R18743 T24577 T24571 punct .,are
R18744 T24578 T24579 punct (,B
R18745 T24579 T24579 ROOT B,B
R18746 T24580 T24579 punct ),B
R18747 T24581 T24583 nsubjpass MDMs,pretreated
R18748 T24582 T24583 auxpass were,pretreated
R18749 T24583 T24583 ROOT pretreated,pretreated
R18750 T24584 T24583 prep with,pretreated
R18751 T24585 T24586 det either,vehicle
R18752 T24586 T24584 pobj vehicle,with
R18753 T24587 T24586 cc or,vehicle
R18754 T24588 T24586 conj Ro-31-8220,vehicle
R18755 T24589 T24583 prep for,pretreated
R18756 T24590 T24591 nummod 30,minutes
R18757 T24591 T24589 pobj minutes,for
R18758 T24592 T24591 prep before,minutes
R18759 T24593 T24594 det the,addition
R18760 T24594 T24592 pobj addition,before
R18761 T24595 T24594 prep of,addition
R18762 T24596 T24595 pobj M-CSF,of
R18763 T24597 T24583 prep for,pretreated
R18764 T24598 T24599 nummod 10,minutes
R18765 T24599 T24597 pobj minutes,for
R18766 T24600 T24583 punct .,pretreated
R18767 T24601 T24602 amod Whole,cell
R18768 T24602 T24603 compound cell,lysates
R18769 T24603 T24605 nsubjpass lysates,resolved
R18770 T24604 T24605 auxpass were,resolved
R18771 T24605 T24605 ROOT resolved,resolved
R18772 T24606 T24605 agent by,resolved
R18773 T24607 T24606 pobj SDS-PAGE,by
R18774 T24608 T24607 cc and,SDS-PAGE
R18775 T24609 T24607 conj phospho-Ser276,SDS-PAGE
R18776 T24610 T24609 cc or,phospho-Ser276
R18777 T24611 T24609 conj phospho-Ser536,phospho-Ser276
R18778 T24612 T24611 prep of,phospho-Ser536
R18779 T24613 T24612 pobj NF-κB,of
R18780 T24614 T24613 nummod p65,NF-κB
R18781 T24615 T24616 auxpass was,detected
R18782 T24616 T24605 conj detected,resolved
R18783 T24617 T24616 advcl using,detected
R18784 T24618 T24619 amod phospho-specific,antibodies
R18785 T24619 T24617 dobj antibodies,using
R18786 T24620 T24617 prep to,using
R18787 T24621 T24622 det either,residue
R18788 T24622 T24620 pobj residue,to
R18789 T24623 T24622 prep of,residue
R18790 T24624 T24625 compound NF-κB,p65
R18791 T24625 T24623 pobj p65,of
R18792 T24626 T24605 punct .,resolved
R18793 T24627 T24628 punct (,C
R18794 T24628 T24632 nmod C,lysates
R18795 T24629 T24628 punct ),C
R18796 T24630 T24631 amod Whole,cell
R18797 T24631 T24632 compound cell,lysates
R18798 T24632 T24651 nsubjpass lysates,subjected
R18799 T24633 T24632 prep of,lysates
R18800 T24634 T24633 pobj RAW,of
R18801 T24635 T24634 nummod 264.7,RAW
R18802 T24636 T24632 conj cells,lysates
R18803 T24637 T24632 acl treated,lysates
R18804 T24638 T24637 prep with,treated
R18805 T24639 T24640 compound vehicle,control
R18806 T24640 T24638 pobj control,with
R18807 T24641 T24640 cc or,control
R18808 T24642 T24640 conj Ro-31-8220,control
R18809 T24643 T24637 prep in,treated
R18810 T24644 T24645 det the,absence
R18811 T24645 T24643 pobj absence,in
R18812 T24646 T24645 cc or,absence
R18813 T24647 T24645 conj presence,absence
R18814 T24648 T24645 prep of,absence
R18815 T24649 T24648 pobj M-CSF,of
R18816 T24650 T24651 auxpass were,subjected
R18817 T24651 T24651 ROOT subjected,subjected
R18818 T24652 T24651 prep to,subjected
R18819 T24653 T24655 amod Western,analysis
R18820 T24654 T24655 compound blot,analysis
R18821 T24655 T24652 pobj analysis,to
R18822 T24656 T24655 prep with,analysis
R18823 T24657 T24656 pobj phospho-Ser276,with
R18824 T24658 T24657 cc or,phospho-Ser276
R18825 T24659 T24660 compound phospho-Ser536,NF-κB
R18826 T24660 T24657 conj NF-κB,phospho-Ser276
R18827 T24661 T24662 nummod p65,antibodies
R18828 T24662 T24656 pobj antibodies,with
R18829 T24663 T24651 punct .,subjected
R18830 T24664 T24667 punct (,Cytosolic
R18831 T24665 T24667 nmod D,Cytosolic
R18832 T24666 T24667 punct ),Cytosolic
R18833 T24667 T24675 nsubjpass Cytosolic,obtained
R18834 T24668 T24667 cc and,Cytosolic
R18835 T24669 T24670 amod nuclear,fractions
R18836 T24670 T24667 conj fractions,Cytosolic
R18837 T24671 T24670 prep of,fractions
R18838 T24672 T24675 nsubjpass RAW,obtained
R18839 T24673 T24672 nummod 264.7,RAW
R18840 T24674 T24675 auxpass were,obtained
R18841 T24675 T24675 ROOT obtained,obtained
R18842 T24676 T24675 prep from,obtained
R18843 T24677 T24679 det the,cells
R18844 T24678 T24679 amod treated,cells
R18845 T24679 T24676 pobj cells,from
R18846 T24680 T24675 cc and,obtained
R18847 T24681 T24675 conj immunoblotted,obtained
R18848 T24682 T24681 prep for,immunoblotted
R18849 T24683 T24682 pobj phospho-NF-κB,for
R18850 T24684 T24675 punct .,obtained
R18851 T24685 T24686 det The,purity
R18852 T24686 T24694 nsubjpass purity,confirmed
R18853 T24687 T24686 prep of,purity
R18854 T24688 T24692 det the,fractions
R18855 T24689 T24692 amod cytosolic,fractions
R18856 T24690 T24689 cc and,cytosolic
R18857 T24691 T24689 conj nuclear,cytosolic
R18858 T24692 T24687 pobj fractions,of
R18859 T24693 T24694 auxpass was,confirmed
R18860 T24694 T24694 ROOT confirmed,confirmed
R18861 T24695 T24694 agent by,confirmed
R18862 T24696 T24695 pcomp immunoblotting,by
R18863 T24697 T24696 prep with,immunoblotting
R18864 T24698 T24697 pobj GAPDH,with
R18865 T24699 T24698 cc and,GAPDH
R18866 T24700 T24701 compound Lamin,B
R18867 T24701 T24698 conj B,GAPDH
R18868 T24702 T24696 punct ",",immunoblotting
R18869 T24703 T24696 advmod respectively,immunoblotting
R18870 T24704 T24694 punct .,confirmed
R18872 T24706 T24705 auxpass are,Shown
R18873 T24707 T24708 compound representative,blots
R18874 T24708 T24705 nsubjpass blots,Shown
R18875 T24709 T24708 prep from,blots
R18876 T24710 T24712 nummod three,experiments
R18877 T24711 T24712 amod independent,experiments
R18878 T24712 T24709 pobj experiments,from
R18879 T24713 T24705 punct .,Shown
R18705 T24539 T24533 prep in,pretreated
R18706 T24540 T24541 det the,absence
R18707 T24541 T24539 pobj absence,in
R18708 T24542 T24541 cc or,absence
R18709 T24543 T24541 conj presence,absence
R18710 T24544 T24541 prep of,absence
R18711 T24545 T24544 pobj Ro-31-8220,of
R18712 T24546 T24533 prep for,pretreated
R18871 T24705 T24705 ROOT Shown,Shown

test2

Id Subject Object Predicate Lexical cue Negation Speculation
T7459 0-5 Protein denotes M-CSF true
T7460 43-51 Regulation denotes Regulate
T7461 72-75 Protein denotes p65
T7462 76-86 Positive_regulation denotes Activation
T7463 136-151 Phosphorylation denotes phosphorylation
T7464 156-167 Protein_catabolism denotes degradation true
T7465 171-175 Protein denotes IκBα
T7466 191-198 Localization denotes release
T7467 203-210 Entity denotes nuclear true
T7468 211-224 Localization denotes translocation
T7469 238-241 Protein denotes p50
T7470 242-245 Protein denotes p65
T7471 360-365 Protein denotes M-CSF
T7472 421-425 Protein denotes IκBα
T7473 426-437 Protein_catabolism denotes degradation
T7474 442-451 Regulation denotes regulated
T7475 507-514 Negative_regulation denotes inhibit
T7476 523-532 Gene_expression denotes synthesis
T7477 536-540 Protein denotes IκBα
T7478 550-561 Protein_catabolism denotes degradation
T7479 638-643 Regulation denotes alter
T7480 644-649 Protein denotes M-CSF
T7481 650-657 Positive_regulation denotes induced
T7482 658-662 Protein denotes IκBα
T7483 663-674 Protein_catabolism denotes degradation
T7484 692-697 Protein denotes M-CSF
T7485 792-823 Protein_modification denotes post-translational modification
T7486 833-836 Protein denotes p65
T24487 882-887 Protein denotes M-CSF true
T24488 918-926 Regulation denotes regulate
T24489 927-931 Protein denotes IκBα
T24490 932-943 Protein_catabolism denotes degradation
T24491 948-957 Regulation denotes regulates true
T24492 962-977 Phosphorylation denotes phosphorylation true
T24493 987-990 Protein denotes p65
T24494 994-1000 Entity denotes Ser276
T24495 1117-1122 Protein denotes M-CSF
T24496 1394-1399 Protein denotes M-CSF
T24497 1483-1490 Phosphorylation denotes phospho
T24498 1507-1510 Protein denotes p65
T24499 1585-1588 Protein denotes p65
T24500 1705-1710 Protein denotes M-CSF
T24501 1756-1763 Phosphorylation denotes phospho
T24502 1774-1781 Phosphorylation denotes phospho
R5522 T7459 T7460 causeOf M-CSF,Regulate
R5523 T7461 T7462 themeOf p65,Activation
R5524 T7462 T7460 themeOf Activation,Regulate
R5525 T7465 T7463 themeOf IκBα,phosphorylation
R5526 T7465 T7464 themeOf IκBα,degradation
R5527 T7467 T7468 locationOf nuclear,translocation
R5528 T7472 T7473 themeOf IκBα,degradation
R5529 T7473 T7474 themeOf degradation,regulated
R5530 T7476 T7475 themeOf synthesis,inhibit
R5531 T7477 T7476 themeOf IκBα,synthesis
R5532 T7477 T7478 themeOf IκBα,degradation
R5533 T7480 T7481 causeOf M-CSF,induced
R5534 T7481 T7479 themeOf induced,alter
R5535 T7482 T7483 themeOf IκBα,degradation
R5536 T7483 T7481 themeOf degradation,induced
R5537 T7486 T7485 themeOf p65,post-translational modification
R18669 T24487 T24488 causeOf M-CSF,regulate
R18670 T24487 T24491 causeOf M-CSF,regulates
R18671 T24489 T24490 themeOf IκBα,degradation
R18672 T24490 T24488 themeOf degradation,regulate
R18673 T24492 T24491 themeOf phosphorylation,regulates
R18674 T24494 T24493 partOf Ser276,p65
R18675 T24494 T24492 themeOf Ser276,phosphorylation