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PMC:3245220 / 16579-17454 JSONTXT

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bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T24373 44-49 P09603 denotes M-CSF
T24374 46-49 P04141 denotes CSF
T24375 60-64 P17252 denotes PKCα
T24376 98-103 P09603 denotes M-CSF
T24377 100-103 P04141 denotes CSF
T24378 473-477 P17252 denotes PKCα
T24379 667-671 P17252 denotes PKCα
T24380 719-724 P09603 denotes M-CSF
T24381 721-724 P04141 denotes CSF
T24382 818-823 P09603 denotes M-CSF
T24383 820-823 P04141 denotes CSF

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T24384 50-59 Positive_regulation denotes activates
T24385 60-64 Protein denotes PKCα
T24386 473-477 Protein denotes PKCα
T24387 667-671 Protein denotes PKCα
T24388 44-49 Protein denotes M-CSF
T24389 98-103 Protein denotes M-CSF
T24390 719-724 Protein denotes M-CSF
T24391 818-823 Protein denotes M-CSF
R18644 T24385 T24384 themeOf PKCα,activates
R18645 T24388 T24384 causeOf M-CSF,activates

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T24459 44-49 Protein denotes M-CSF
T24460 60-64 Protein denotes PKCα
T24461 50-59 Positive_regulation denotes activates
T24462 98-103 Protein denotes M-CSF
T24463 184-201 Protein denotes anti-PKC antibody
T24464 205-225 Protein denotes control IgG antibody
T24465 271-281 Protein denotes PKC kinase
T24466 473-477 Protein denotes PKCα
T24467 601-604 Protein denotes Rad
T24468 667-671 Protein denotes PKCα
T24469 719-724 Protein denotes M-CSF
T24470 818-823 Protein denotes M-CSF
R18658 T24459 T24461 causeOf M-CSF,activates
R18659 T24460 T24461 themeOf PKCα,activates

events-check-again

Id Subject Object Predicate Lexical cue
T24451 44-49 Protein denotes M-CSF
T24452 50-59 Positive_regulation denotes activates
T24453 60-64 Protein denotes PKCα
T24454 98-103 Protein denotes M-CSF
T24455 473-477 Protein denotes PKCα
T24456 667-671 Protein denotes PKCα
T24457 719-724 Protein denotes M-CSF
T24458 818-823 Protein denotes M-CSF
R18656 T24451 T24452 causeOf M-CSF,activates
R18657 T24453 T24452 themeOf PKCα,activates

GO-CC

Id Subject Object Predicate Lexical cue
T24405 193-201 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T24406 217-225 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T24407 193-201 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T24408 217-225 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T24409 213-225 http://purl.obolibrary.org/obo/GO_0071736 denotes IgG antibody

GO-MF

Id Subject Object Predicate Lexical cue
T24398 60-64 http://purl.obolibrary.org/obo/GO_0004697 denotes PKCα
T24399 473-477 http://purl.obolibrary.org/obo/GO_0004697 denotes PKCα
T24400 189-192 http://purl.obolibrary.org/obo/GO_0004697 denotes PKC
T24401 271-274 http://purl.obolibrary.org/obo/GO_0004697 denotes PKC
T24402 193-201 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T24403 217-225 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T24404 275-290 http://purl.obolibrary.org/obo/GO_0016301 denotes kinase activity

GO-BP

Id Subject Object Predicate Lexical cue
T24471 44-59 http://purl.obolibrary.org/obo/GO_1901258 denotes M-CSF activates
T24472 44-59 http://purl.obolibrary.org/obo/GO_1903971 denotes M-CSF activates
T24473 275-290 http://purl.obolibrary.org/obo/GO_0016301 denotes kinase activity

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T24238 44-49 NN denotes M-CSF
T24239 50-59 VB denotes activates
T24240 60-64 NN denotes PKCα
T24241 65-67 IN denotes in
T24242 68-73 JJ denotes human
T24243 74-78 NN denotes MDMs
T24244 80-84 NN denotes MDMs
T24245 85-92 VB denotes treated
T24246 93-97 IN denotes with
T24247 98-103 NN denotes M-CSF
T24248 104-105 -LRB- denotes (
T24249 105-108 CD denotes 100
T24250 109-114 NN denotes ng/ml
T24251 114-115 -RRB- denotes )
T24252 116-119 IN denotes for
T24253 120-127 VB denotes varying
T24254 128-135 NN denotes amounts
T24255 136-138 IN denotes of
T24256 139-143 NN denotes time
T24257 144-148 VB denotes were
T24258 149-154 VB denotes lysed
T24259 155-158 CC denotes and
T24260 159-177 VB denotes immunoprecipitated
T24261 178-183 VB denotes using
T24262 184-192 JJ denotes anti-PKC
T24263 193-201 NN denotes antibody
T24264 202-204 CC denotes or
T24265 205-212 NN denotes control
T24266 213-216 NN denotes IgG
T24267 217-225 NN denotes antibody
T24268 227-230 CD denotes One
T24269 231-235 NN denotes half
T24270 236-238 IN denotes of
T24271 239-242 DT denotes the
T24272 243-250 NN denotes samples
T24273 251-254 VB denotes was
T24274 255-259 VB denotes used
T24275 260-262 TO denotes to
T24276 263-270 VB denotes analyze
T24277 271-274 NN denotes PKC
T24278 275-281 NN denotes kinase
T24279 282-290 NN denotes activity
T24280 291-296 VB denotes using
T24281 297-298 DT denotes a
T24282 299-310 NN denotes fluorescein
T24283 311-317 VB denotes tagged
T24284 318-325 NN denotes peptide
T24285 326-329 CC denotes and
T24286 330-340 VB denotes visualized
T24287 341-343 IN denotes by
T24288 344-351 NN denotes agarose
T24289 352-355 NN denotes gel
T24290 356-371 NN denotes electrophoresis
T24291 372-373 -LRB- denotes (
T24292 373-376 JJ denotes top
T24293 377-382 NN denotes panel
T24294 382-383 -RRB- denotes )
T24295 383-384 -COMMA- denotes ,
T24296 385-390 IN denotes while
T24297 391-394 DT denotes the
T24298 395-400 JJ denotes other
T24299 401-405 NN denotes half
T24300 406-409 VB denotes was
T24301 410-419 VB denotes subjected
T24302 420-422 TO denotes to
T24303 423-430 JJ denotes Western
T24304 431-435 NN denotes blot
T24305 436-444 NN denotes analysis
T24306 445-447 TO denotes to
T24307 448-455 VB denotes confirm
T24308 456-461 JJ denotes equal
T24309 462-469 NN denotes amounts
T24310 470-472 IN denotes of
T24311 473-477 NN denotes PKCα
T24312 478-482 VB denotes were
T24313 483-501 VB denotes immunoprecipitated
T24314 502-506 IN denotes from
T24315 507-511 DT denotes each
T24316 512-518 NN denotes sample
T24317 519-520 -LRB- denotes (
T24318 520-526 JJ denotes middle
T24319 527-532 NN denotes panel
T24320 532-533 -RRB- denotes )
T24321 535-538 DT denotes The
T24322 539-545 NN denotes kinase
T24323 546-551 NN denotes assay
T24324 552-555 VB denotes was
T24325 556-567 VB denotes quantitated
T24326 568-573 VB denotes using
T24327 574-582 NN denotes Quantity
T24328 583-586 CD denotes One
T24329 587-595 NN denotes software
T24330 596-597 -LRB- denotes (
T24331 597-604 NN denotes Bio-Rad
T24332 604-605 -RRB- denotes )
T24333 606-607 -LRB- denotes (
T24334 607-613 JJ denotes bottom
T24335 614-619 NN denotes panel
T24336 619-620 -RRB- denotes )
T24337 622-626 NN denotes Data
T24338 627-637 VB denotes represents
T24339 638-641 DT denotes the
T24340 642-649 JJ denotes average
T24341 650-654 JJ denotes fold
T24342 655-663 NN denotes increase
T24343 664-666 IN denotes of
T24344 667-671 NN denotes PKCα
T24345 672-680 NN denotes activity
T24346 681-683 IN denotes in
T24347 684-698 JJ denotes non-stimulated
T24348 699-706 NN denotes samples
T24349 707-715 VB denotes compared
T24350 716-718 TO denotes to
T24351 719-732 JJ denotes M-CSF-treated
T24352 733-736 NN denotes MDM
T24353 737-738 NN denotes ±
T24354 739-744 NN denotes S.E.M
T24355 745-748 IN denotes for
T24356 749-754 CD denotes three
T24357 755-766 JJ denotes independent
T24358 767-778 NN denotes experiments
T24359 780-782 NN denotes NS
T24360 782-783 -COLON- denotes :
T24361 784-799 JJ denotes non-stimulated.
T24362 800-801 SYM denotes *
T24363 802-805 DT denotes The
T24364 806-814 NN denotes p-values
T24365 815-817 IN denotes of
T24366 818-823 NN denotes M-CSF
T24367 824-834 VB denotes stimulated
T24368 835-843 VB denotes compared
T24369 844-846 TO denotes to
T24370 847-861 JJ denotes non-stimulated
T24371 862-866 VB denotes were
T24372 867-872 CD denotes ≤0.05
R18506 T24238 T24239 arg1Of M-CSF,activates
R18507 T24240 T24239 arg2Of PKCα,activates
R18508 T24240 T24241 arg1Of PKCα,in
R18509 T24243 T24241 arg2Of MDMs,in
R18510 T24243 T24242 arg1Of MDMs,human
R18511 T24244 T24245 arg2Of MDMs,treated
R18512 T24244 T24257 arg1Of MDMs,were
R18513 T24244 T24258 arg2Of MDMs,lysed
R18514 T24244 T24260 arg2Of MDMs,immunoprecipitated
R18515 T24244 T24261 arg1Of MDMs,using
R18516 T24245 T24246 arg1Of treated,with
R18517 T24247 T24246 arg2Of M-CSF,with
R18518 T24247 T24248 arg1Of M-CSF,(
R18519 T24247 T24252 arg1Of M-CSF,for
R18520 T24250 T24248 arg2Of ng/ml,(
R18521 T24250 T24249 arg1Of ng/ml,100
R18522 T24251 T24248 arg3Of ),(
R18523 T24254 T24252 arg2Of amounts,for
R18524 T24254 T24253 arg1Of amounts,varying
R18525 T24254 T24255 arg1Of amounts,of
R18526 T24256 T24255 arg2Of time,of
R18527 T24258 T24259 arg1Of lysed,and
R18528 T24259 T24257 arg2Of and,were
R18529 T24260 T24259 arg2Of immunoprecipitated,and
R18530 T24261 T24260 arg3Of using,immunoprecipitated
R18531 T24263 T24262 arg1Of antibody,anti-PKC
R18532 T24263 T24264 arg1Of antibody,or
R18533 T24264 T24261 arg2Of or,using
R18534 T24267 T24264 arg2Of antibody,or
R18535 T24267 T24265 arg1Of antibody,control
R18536 T24267 T24266 arg1Of antibody,IgG
R18537 T24269 T24268 arg1Of half,One
R18538 T24269 T24270 arg1Of half,of
R18539 T24269 T24273 arg1Of half,was
R18540 T24269 T24274 arg2Of half,used
R18541 T24272 T24270 arg2Of samples,of
R18542 T24272 T24271 arg1Of samples,the
R18543 T24274 T24273 arg2Of used,was
R18544 T24276 T24274 arg3Of analyze,used
R18545 T24276 T24275 arg1Of analyze,to
R18546 T24279 T24276 arg2Of activity,analyze
R18547 T24279 T24277 arg1Of activity,PKC
R18548 T24279 T24278 arg1Of activity,kinase
R18549 T24279 T24280 arg1Of activity,using
R18550 T24282 T24280 arg2Of fluorescein,using
R18551 T24282 T24281 arg1Of fluorescein,a
R18552 T24282 T24283 arg2Of fluorescein,tagged
R18553 T24282 T24286 arg2Of fluorescein,visualized
R18554 T24283 T24285 arg1Of tagged,and
R18555 T24284 T24283 arg3Of peptide,tagged
R18556 T24285 T24295 arg1Of and,","
R18557 T24285 T24296 arg1Of and,while
R18558 T24286 T24285 arg2Of visualized,and
R18559 T24290 T24286 arg1Of electrophoresis,visualized
R18560 T24290 T24287 arg2Of electrophoresis,by
R18561 T24290 T24288 arg1Of electrophoresis,agarose
R18562 T24290 T24289 arg1Of electrophoresis,gel
R18563 T24290 T24291 arg1Of electrophoresis,(
R18564 T24293 T24291 arg2Of panel,(
R18565 T24293 T24292 arg1Of panel,top
R18566 T24294 T24291 arg3Of ),(
R18567 T24299 T24297 arg1Of half,the
R18568 T24299 T24298 arg1Of half,other
R18569 T24299 T24300 arg1Of half,was
R18570 T24299 T24301 arg2Of half,subjected
R18571 T24301 T24296 arg2Of subjected,while
R18572 T24301 T24300 arg2Of subjected,was
R18573 T24301 T24302 arg1Of subjected,to
R18574 T24301 T24306 modOf subjected,to
R18575 T24305 T24302 arg2Of analysis,to
R18576 T24305 T24303 arg1Of analysis,Western
R18577 T24305 T24304 arg1Of analysis,blot
R18578 T24307 T24306 arg1Of confirm,to
R18579 T24309 T24308 arg1Of amounts,equal
R18580 T24309 T24310 arg1Of amounts,of
R18581 T24309 T24312 arg1Of amounts,were
R18582 T24309 T24313 arg2Of amounts,immunoprecipitated
R18583 T24311 T24310 arg2Of PKCα,of
R18584 T24313 T24307 arg2Of immunoprecipitated,confirm
R18585 T24313 T24312 arg2Of immunoprecipitated,were
R18586 T24313 T24314 arg1Of immunoprecipitated,from
R18587 T24316 T24314 arg2Of sample,from
R18588 T24316 T24315 arg1Of sample,each
R18589 T24316 T24317 arg1Of sample,(
R18590 T24319 T24317 arg2Of panel,(
R18591 T24319 T24318 arg1Of panel,middle
R18592 T24320 T24317 arg3Of ),(
R18593 T24323 T24321 arg1Of assay,The
R18594 T24323 T24322 arg1Of assay,kinase
R18595 T24323 T24324 arg1Of assay,was
R18596 T24323 T24325 arg2Of assay,quantitated
R18597 T24325 T24324 arg2Of quantitated,was
R18598 T24326 T24325 arg3Of using,quantitated
R18599 T24329 T24326 arg2Of software,using
R18600 T24329 T24327 arg1Of software,Quantity
R18601 T24329 T24328 arg1Of software,One
R18602 T24329 T24330 arg1Of software,(
R18603 T24329 T24333 arg1Of software,(
R18604 T24331 T24330 arg2Of Bio-Rad,(
R18605 T24332 T24330 arg3Of ),(
R18606 T24335 T24333 arg2Of panel,(
R18607 T24335 T24334 arg1Of panel,bottom
R18608 T24336 T24333 arg3Of ),(
R18609 T24337 T24338 arg1Of Data,represents
R18610 T24338 T24349 arg1Of represents,compared
R18611 T24342 T24338 arg2Of increase,represents
R18612 T24342 T24339 arg1Of increase,the
R18613 T24342 T24340 arg1Of increase,average
R18614 T24342 T24341 arg1Of increase,fold
R18615 T24342 T24343 arg1Of increase,of
R18616 T24342 T24346 arg1Of increase,in
R18617 T24345 T24343 arg2Of activity,of
R18618 T24345 T24344 arg1Of activity,PKCα
R18619 T24348 T24346 arg2Of samples,in
R18620 T24348 T24347 arg1Of samples,non-stimulated
R18621 T24350 T24349 arg2Of to,compared
R18622 T24354 T24350 arg2Of S.E.M,to
R18623 T24354 T24351 arg1Of S.E.M,M-CSF-treated
R18624 T24354 T24352 arg1Of S.E.M,MDM
R18625 T24354 T24353 arg1Of S.E.M,±
R18626 T24354 T24355 arg1Of S.E.M,for
R18627 T24358 T24355 arg2Of experiments,for
R18628 T24358 T24356 arg1Of experiments,three
R18629 T24358 T24357 arg1Of experiments,independent
R18630 T24359 T24360 arg1Of NS,:
R18631 T24359 T24361 arg1Of NS,non-stimulated.
R18632 T24359 T24371 modOf NS,were
R18633 T24364 T24362 arg1Of p-values,*
R18634 T24364 T24363 arg1Of p-values,The
R18635 T24364 T24365 arg1Of p-values,of
R18636 T24364 T24371 arg1Of p-values,were
R18637 T24364 T24372 arg1Of p-values,≤0.05
R18638 T24366 T24365 arg2Of M-CSF,of
R18639 T24366 T24367 arg2Of M-CSF,stimulated
R18640 T24367 T24368 arg1Of stimulated,compared
R18641 T24369 T24368 arg2Of to,compared
R18642 T24370 T24369 arg2Of non-stimulated,to
R18643 T24372 T24371 arg2Of ≤0.05,were

sentences

Id Subject Object Predicate Lexical cue
T24089 44-79 Sentence denotes M-CSF activates PKCα in human MDMs.
T24090 80-226 Sentence denotes MDMs treated with M-CSF (100 ng/ml) for varying amounts of time were lysed and immunoprecipitated using anti-PKC antibody or control IgG antibody.
T24091 227-534 Sentence denotes One half of the samples was used to analyze PKC kinase activity using a fluorescein tagged peptide and visualized by agarose gel electrophoresis (top panel), while the other half was subjected to Western blot analysis to confirm equal amounts of PKCα were immunoprecipitated from each sample (middle panel).
T24092 535-621 Sentence denotes The kinase assay was quantitated using Quantity One software (Bio-Rad) (bottom panel).
T24093 622-779 Sentence denotes Data represents the average fold increase of PKCα activity in non-stimulated samples compared to M-CSF-treated MDM ± S.E.M for three independent experiments.
T24094 780-873 Sentence denotes NS: non-stimulated. * The p-values of M-CSF stimulated compared to non-stimulated were ≤0.05.
T122 0-79 Sentence denotes 10.1371/journal.pone.0028081.g004 Figure 4 M-CSF activates PKCα in human MDMs.
T123 80-226 Sentence denotes MDMs treated with M-CSF (100 ng/ml) for varying amounts of time were lysed and immunoprecipitated using anti-PKC antibody or control IgG antibody.
T124 227-534 Sentence denotes One half of the samples was used to analyze PKC kinase activity using a fluorescein tagged peptide and visualized by agarose gel electrophoresis (top panel), while the other half was subjected to Western blot analysis to confirm equal amounts of PKCα were immunoprecipitated from each sample (middle panel).
T125 535-621 Sentence denotes The kinase assay was quantitated using Quantity One software (Bio-Rad) (bottom panel).
T126 622-779 Sentence denotes Data represents the average fold increase of PKCα activity in non-stimulated samples compared to M-CSF-treated MDM ± S.E.M for three independent experiments.
T127 780-873 Sentence denotes NS: non-stimulated. * The p-values of M-CSF stimulated compared to non-stimulated were ≤0.05.

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T24095 44-49 NNP denotes M-CSF
T24096 50-59 VBZ denotes activates
T24097 60-64 NNP denotes PKCα
T24098 65-67 IN denotes in
T24099 68-73 JJ denotes human
T24100 74-78 NNS denotes MDMs
T24101 78-79 . denotes .
T24102 80-84 NNS denotes MDMs
T24103 85-92 VBN denotes treated
T24104 93-97 IN denotes with
T24105 98-103 NNP denotes M-CSF
T24106 104-105 -LRB- denotes (
T24107 105-108 CD denotes 100
T24108 109-114 NN denotes ng/ml
T24109 114-115 -RRB- denotes )
T24110 116-119 IN denotes for
T24111 120-127 VBG denotes varying
T24112 128-135 NNS denotes amounts
T24113 136-138 IN denotes of
T24114 139-143 NN denotes time
T24115 144-148 VBD denotes were
T24116 149-154 VBN denotes lysed
T24117 155-158 CC denotes and
T24118 159-177 VBN denotes immunoprecipitated
T24119 178-183 VBG denotes using
T24120 184-192 JJ denotes anti-PKC
T24121 193-201 NN denotes antibody
T24122 202-204 CC denotes or
T24123 205-212 NN denotes control
T24124 213-216 NNP denotes IgG
T24125 217-225 NN denotes antibody
T24126 225-226 . denotes .
T24127 227-230 CD denotes One
T24128 231-235 NN denotes half
T24129 236-238 IN denotes of
T24130 239-242 DT denotes the
T24131 243-250 NNS denotes samples
T24132 251-254 VBD denotes was
T24133 255-259 VBN denotes used
T24134 260-262 TO denotes to
T24135 263-270 VB denotes analyze
T24136 271-274 NNP denotes PKC
T24137 275-281 NN denotes kinase
T24138 282-290 NN denotes activity
T24139 291-296 VBG denotes using
T24140 297-298 DT denotes a
T24141 299-310 NN denotes fluorescein
T24142 311-317 VBD denotes tagged
T24143 318-325 NN denotes peptide
T24144 326-329 CC denotes and
T24145 330-340 VBN denotes visualized
T24146 341-343 IN denotes by
T24147 344-351 JJ denotes agarose
T24148 352-355 NN denotes gel
T24149 356-371 NN denotes electrophoresis
T24150 372-373 -LRB- denotes (
T24151 373-376 JJ denotes top
T24152 377-382 NN denotes panel
T24153 382-383 -RRB- denotes )
T24154 383-384 , denotes ,
T24155 385-390 IN denotes while
T24156 391-394 DT denotes the
T24157 395-400 JJ denotes other
T24158 401-405 NN denotes half
T24159 406-409 VBD denotes was
T24160 410-419 VBN denotes subjected
T24161 420-422 TO denotes to
T24162 423-430 JJ denotes Western
T24163 431-435 NN denotes blot
T24164 436-444 NN denotes analysis
T24165 445-447 TO denotes to
T24166 448-455 VB denotes confirm
T24167 456-461 JJ denotes equal
T24168 462-469 NNS denotes amounts
T24169 470-472 IN denotes of
T24170 473-477 NNP denotes PKCα
T24171 478-482 VBD denotes were
T24172 483-501 VBN denotes immunoprecipitated
T24173 502-506 IN denotes from
T24174 507-511 DT denotes each
T24175 512-518 NN denotes sample
T24176 519-520 -LRB- denotes (
T24177 520-526 JJ denotes middle
T24178 527-532 NN denotes panel
T24179 532-533 -RRB- denotes )
T24180 533-534 . denotes .
T24181 535-538 DT denotes The
T24182 539-545 NN denotes kinase
T24183 546-551 NN denotes assay
T24184 552-555 VBD denotes was
T24185 556-567 VBN denotes quantitated
T24186 568-573 VBG denotes using
T24187 574-582 NNP denotes Quantity
T24188 583-586 CD denotes One
T24189 587-595 NN denotes software
T24190 596-597 -LRB- denotes (
T24191 597-604 NNP denotes Bio-Rad
T24192 604-605 -RRB- denotes )
T24193 606-607 -LRB- denotes (
T24194 607-613 JJ denotes bottom
T24195 614-619 NN denotes panel
T24196 619-620 -RRB- denotes )
T24197 620-621 . denotes .
T24198 622-626 NNP denotes Data
T24199 627-637 VBZ denotes represents
T24200 638-641 DT denotes the
T24201 642-649 JJ denotes average
T24202 650-654 VBP denotes fold
T24203 655-663 NN denotes increase
T24204 664-666 IN denotes of
T24205 667-671 NN denotes PKCα
T24206 672-680 NN denotes activity
T24207 681-683 IN denotes in
T24208 684-698 JJ denotes non-stimulated
T24209 699-706 NNS denotes samples
T24210 707-715 VBN denotes compared
T24211 716-718 TO denotes to
T24212 719-732 JJ denotes M-CSF-treated
T24213 733-736 NNP denotes MDM
T24214 737-738 NN denotes ±
T24215 739-744 NNP denotes S.E.M
T24216 745-748 IN denotes for
T24217 749-754 CD denotes three
T24218 755-766 JJ denotes independent
T24219 767-778 NNS denotes experiments
T24220 778-779 . denotes .
T24221 780-782 NNP denotes NS
T24222 782-783 : denotes :
T24223 784-798 JJ denotes non-stimulated
T24224 798-799 . denotes .
T24225 800-801 SYM denotes *
T24226 802-805 DT denotes The
T24227 806-814 NNS denotes p-values
T24228 815-817 IN denotes of
T24229 818-823 NNP denotes M-CSF
T24230 824-834 VBD denotes stimulated
T24231 835-843 VBN denotes compared
T24232 844-846 TO denotes to
T24233 847-861 JJ denotes non-stimulated
T24234 862-866 VBD denotes were
T24235 867-868 CD denotes ≤
T24236 868-872 CD denotes 0.05
T24237 872-873 . denotes .
R18363 T24095 T24096 nsubj M-CSF,activates
R18364 T24096 T24096 ROOT activates,activates
R18365 T24097 T24096 dobj PKCα,activates
R18366 T24098 T24096 prep in,activates
R18367 T24099 T24100 amod human,MDMs
R18368 T24100 T24098 pobj MDMs,in
R18369 T24101 T24096 punct .,activates
R18370 T24102 T24116 nsubjpass MDMs,lysed
R18371 T24103 T24102 acl treated,MDMs
R18372 T24104 T24103 prep with,treated
R18373 T24105 T24104 pobj M-CSF,with
R18374 T24106 T24108 punct (,ng/ml
R18375 T24107 T24108 nummod 100,ng/ml
R18376 T24108 T24105 appos ng/ml,M-CSF
R18377 T24109 T24103 punct ),treated
R18378 T24110 T24103 prep for,treated
R18379 T24111 T24112 amod varying,amounts
R18380 T24112 T24110 pobj amounts,for
R18381 T24113 T24112 prep of,amounts
R18382 T24114 T24113 pobj time,of
R18383 T24115 T24116 auxpass were,lysed
R18384 T24116 T24116 ROOT lysed,lysed
R18385 T24117 T24116 cc and,lysed
R18386 T24118 T24116 conj immunoprecipitated,lysed
R18387 T24119 T24118 advcl using,immunoprecipitated
R18388 T24120 T24121 amod anti-PKC,antibody
R18389 T24121 T24119 dobj antibody,using
R18390 T24122 T24121 cc or,antibody
R18391 T24123 T24121 conj control,antibody
R18392 T24124 T24125 compound IgG,antibody
R18393 T24125 T24121 conj antibody,antibody
R18394 T24126 T24116 punct .,lysed
R18395 T24127 T24128 nummod One,half
R18396 T24128 T24133 nsubjpass half,used
R18397 T24129 T24128 prep of,half
R18398 T24130 T24131 det the,samples
R18399 T24131 T24129 pobj samples,of
R18400 T24132 T24133 auxpass was,used
R18401 T24133 T24133 ROOT used,used
R18402 T24134 T24135 aux to,analyze
R18403 T24135 T24133 xcomp analyze,used
R18404 T24136 T24138 compound PKC,activity
R18405 T24137 T24138 compound kinase,activity
R18406 T24138 T24135 dobj activity,analyze
R18407 T24139 T24135 advcl using,analyze
R18408 T24140 T24141 det a,fluorescein
R18409 T24141 T24139 dobj fluorescein,using
R18410 T24142 T24133 conj tagged,used
R18411 T24143 T24142 dobj peptide,tagged
R18412 T24144 T24142 cc and,tagged
R18413 T24145 T24142 conj visualized,tagged
R18414 T24146 T24145 agent by,visualized
R18415 T24147 T24149 amod agarose,electrophoresis
R18416 T24148 T24149 compound gel,electrophoresis
R18417 T24149 T24146 pobj electrophoresis,by
R18418 T24150 T24149 punct (,electrophoresis
R18419 T24151 T24152 amod top,panel
R18420 T24152 T24149 appos panel,electrophoresis
R18421 T24153 T24149 punct ),electrophoresis
R18422 T24154 T24142 punct ",",tagged
R18423 T24155 T24160 mark while,subjected
R18424 T24156 T24158 det the,half
R18425 T24157 T24158 amod other,half
R18426 T24158 T24160 nsubjpass half,subjected
R18427 T24159 T24160 auxpass was,subjected
R18428 T24160 T24142 advcl subjected,tagged
R18429 T24161 T24160 prep to,subjected
R18430 T24162 T24164 amod Western,analysis
R18431 T24163 T24164 compound blot,analysis
R18432 T24164 T24161 pobj analysis,to
R18433 T24165 T24166 aux to,confirm
R18434 T24166 T24160 xcomp confirm,subjected
R18435 T24167 T24168 amod equal,amounts
R18436 T24168 T24166 dobj amounts,confirm
R18437 T24169 T24168 prep of,amounts
R18438 T24170 T24169 pobj PKCα,of
R18439 T24171 T24172 auxpass were,immunoprecipitated
R18440 T24172 T24142 conj immunoprecipitated,tagged
R18441 T24173 T24172 prep from,immunoprecipitated
R18442 T24174 T24175 det each,sample
R18443 T24175 T24173 pobj sample,from
R18444 T24176 T24178 punct (,panel
R18445 T24177 T24178 amod middle,panel
R18446 T24178 T24175 appos panel,sample
R18447 T24179 T24172 punct ),immunoprecipitated
R18448 T24180 T24133 punct .,used
R18449 T24181 T24183 det The,assay
R18450 T24182 T24183 compound kinase,assay
R18451 T24183 T24185 nsubjpass assay,quantitated
R18452 T24184 T24185 auxpass was,quantitated
R18453 T24185 T24185 ROOT quantitated,quantitated
R18454 T24186 T24185 advcl using,quantitated
R18455 T24187 T24186 dobj Quantity,using
R18456 T24188 T24189 nummod One,software
R18457 T24189 T24195 nmod software,panel
R18458 T24190 T24191 punct (,Bio-Rad
R18459 T24191 T24189 appos Bio-Rad,software
R18460 T24192 T24189 punct ),software
R18461 T24193 T24195 punct (,panel
R18462 T24194 T24195 amod bottom,panel
R18463 T24195 T24187 appos panel,Quantity
R18464 T24196 T24187 punct ),Quantity
R18465 T24197 T24185 punct .,quantitated
R18466 T24198 T24199 nsubj Data,represents
R18467 T24199 T24199 ROOT represents,represents
R18468 T24200 T24203 det the,increase
R18469 T24201 T24202 amod average,fold
R18470 T24202 T24203 compound fold,increase
R18471 T24203 T24199 dobj increase,represents
R18472 T24204 T24203 prep of,increase
R18473 T24205 T24206 compound PKCα,activity
R18474 T24206 T24204 pobj activity,of
R18475 T24207 T24206 prep in,activity
R18476 T24208 T24209 amod non-stimulated,samples
R18477 T24209 T24207 pobj samples,in
R18478 T24210 T24199 prep compared,represents
R18479 T24211 T24210 prep to,compared
R18480 T24212 T24215 compound M-CSF-treated,S.E.M
R18481 T24213 T24214 compound MDM,±
R18482 T24214 T24215 compound ±,S.E.M
R18483 T24215 T24211 pobj S.E.M,to
R18484 T24216 T24210 prep for,compared
R18485 T24217 T24219 nummod three,experiments
R18486 T24218 T24219 amod independent,experiments
R18487 T24219 T24216 pobj experiments,for
R18488 T24220 T24199 punct .,represents
R18489 T24221 T24221 ROOT NS,NS
R18490 T24222 T24221 punct :,NS
R18491 T24223 T24221 acl non-stimulated,NS
R18492 T24224 T24221 punct .,NS
R18493 T24225 T24225 ROOT *,*
R18494 T24226 T24227 det The,p-values
R18495 T24227 T24230 nsubj p-values,stimulated
R18496 T24228 T24227 prep of,p-values
R18497 T24229 T24228 pobj M-CSF,of
R18498 T24230 T24230 ROOT stimulated,stimulated
R18499 T24231 T24230 prep compared,stimulated
R18500 T24232 T24231 prep to,compared
R18501 T24233 T24232 pobj non-stimulated,to
R18502 T24234 T24230 conj were,stimulated
R18503 T24235 T24236 compound ≤,0.05
R18504 T24236 T24234 attr 0.05,were
R18505 T24237 T24230 punct .,stimulated

test2

Id Subject Object Predicate Lexical cue
T24080 44-49 Protein denotes M-CSF
T24081 50-59 Positive_regulation denotes activates
T24082 60-64 Protein denotes PKCα
T24083 98-103 Protein denotes M-CSF
T24084 473-477 Protein denotes PKCα
T24085 655-663 Positive_regulation denotes increase
T24086 667-671 Protein denotes PKCα
T24087 719-724 Protein denotes M-CSF
T24088 818-823 Protein denotes M-CSF
R18359 T24080 T24081 causeOf M-CSF,activates
R18360 T24082 T24081 themeOf PKCα,activates
R18361 T24086 T24085 themeOf PKCα,increase
R18362 T24087 T24085 causeOf M-CSF,increase