PMC:3220857 / 9408-13142 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PMC/sourceid/3220857","sourcedb":"PMC","sourceid":"3220857","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3220857","text":"RESULTS\nIn line with our previous observations in 12 patients (11), a subset of the 27 patients who were included in the current study, the VLCD profoundly reduced bodyweight from 113.1 ± 3.7 to 87.7 ± 2.9 kg (P \u003c 0.05) and decreased BMI from 37.2 ± 0.9 to 28.9 ± 0.8 kg/m2 (P \u003c 0.05). In addition, in the 12 patients from whom 1H-MRS scans could be obtained, hepatic TG content reduced considerably from 21.2 ± 4.2 to 3.0 ± 0.9% (n = 12; P \u003c 0.001) as reported previously (11).\n\nPlasma CETP and (apo)lipoproteins\nWhen compared with baseline, VLCD decreased plasma CETP concentration (−18.2%; P \u003c 0.01). In addition, VLCD reduced plasma levels of total cholesterol (−13.1%; P \u003c 0.001), TG (−45.1%; P \u003c 0.001), phospholipid (−15.2%; P \u003c 0.0001), LDL-cholesterol (−15.8%; P \u003c 0.01), and apoB100 (−13.9%; P \u003c 0.01). VLCD did not alter plasma HDL-cholesterol and HDL-phospholipids, but increased apoAI (+16.2%; P \u003c 0.05) (Table 1). The change in body weight after 16 weeks of VLCD did not correlate with the change in either plasma TG (R2 = 0.0000; P = 0.9952), total cholesterol (R2 = 0.0471; P = 0.2769), phospholipid (R2 = 0.0305; P = 0.3837), LDL-cholesterol (R2 = 0.0320; P = 0.3717), HDL-cholesterol (R2 = 0.0086; P = 0.6460), or HDL-phospholipid (R2 = 0.0013; P = 0.8570).\nTable 1 Plasma CETP and (apo)lipoprotein levels in obese patients with type 2 diabetes mellitus and hepatic steatosis in response to 16 weeks of VLCD\nPlasma parameters Baseline After VLCD Δ (%) P value\nCETP (μg/mL) 2.48 ± 0.15 2.03 ± 0.14 −18.2 0.0021\nTotal cholesterol (mmol/L) 5.76 ± 0.30 5.00 ± 0.22 −13.1 0.0007\nTriglycerides (mmol/L) 2.41 ± 0.28 1.32 ± 0.10 −45.1 0.0003\nPhospholipids (mmol/L) 2.69 ± 0.11 2.28 ± 0.07 −15.2 0.0000\nLDL-cholesterol (mmol/L) 3.99 ± 0.27 3.36 ± 0.20 −15.8 0.0028\nApoB100 (mg/dL) 130 ± 6 111 ± 5 −13.9 0.0016\nHDL-cholesterol (mmol/L) 0.84 ± 0.04 0.91 ± 0.06 — NS\nHDL-phospholipids (mmol/L) 0.98 ± 0.03 0.98 ± 0.05 — NS\nApoAI (mg/dL) 135 ± 10 156 ± 11 +16.2 0.0174\nData are presented as means ± SEM (n = 27). Δ values are calculated by comparing values obtained after VLCD with those obtained at baseline from obese patients with type 2 diabetes mellitus. P values are calculated using paired Student t test. NS, not significant.\n\nCholesterol efflux\nVLCD decreased cholesterol efflux from THP-1 cells to total plasma obtained from patients compared with plasma from baseline (−14.5%; P \u003c 0.001; Fig. 1A). Similarly, the capacity of apoB-depleted plasma obtained after VLCD to promote cholesterol efflux was lower than that of apoB-depleted plasma obtained at baseline (−14.9%; P \u003c 0.001; Fig. 1B).\nFigure 1 Cholesterol efflux to total plasma and apoB-depleted plasma. THP-1 cells were loaded with [3H]cholesterol and incubated for 4 h at 37°C with total plasma (1% vol/vol; A) or apoB-depleted plasma (1% vol/vol; B), obtained before and after VLCD from 27 obese patients with type 2 diabetes mellitus. Cholesterol efflux rate is calculated by dividing 3H-activity in the medium by the sum of the 3H-radioactivity in the medium and cell extract. Data are means ± SEM. P values are calculated using paired Student t test. ***P \u003c 0.001 as compared with baseline.Correlation analysis showed that cholesterol efflux to total plasma positively correlated with plasma total cholesterol (R2 = 0.2416; P \u003c 0.001) and plasma total phospholipid (R2 = 0.3499; P \u003c 0.001). Cholesterol efflux to total plasma positively correlated with non-HDL-cholesterol (R2 = 0.2339; P \u003c 0.001) and non-HDL-phospholipid (R2 = 0.2855; P \u003c 0.001) rather than HDL-cholesterol or HDL-phospholipid (both P \u003e 0.05; Supplementary Fig. 1). Moreover, no significant correlation was observed between cholesterol efflux to plasma and apoAI (Supplementary Fig. 2).","divisions":[{"label":"title","span":{"begin":0,"end":7}},{"label":"p","span":{"begin":8,"end":478}},{"label":"sec","span":{"begin":480,"end":2238}},{"label":"title","span":{"begin":480,"end":513}},{"label":"p","span":{"begin":514,"end":1275}},{"label":"table-wrap","span":{"begin":1276,"end":2238}},{"label":"label","span":{"begin":1276,"end":1283}},{"label":"caption","span":{"begin":1284,"end":1425}},{"label":"p","span":{"begin":1284,"end":1425}},{"label":"table","span":{"begin":1426,"end":1973}},{"label":"tr","span":{"begin":1426,"end":1477}},{"label":"th","span":{"begin":1426,"end":1443}},{"label":"th","span":{"begin":1444,"end":1452}},{"label":"th","span":{"begin":1453,"end":1463}},{"label":"th","span":{"begin":1464,"end":1469}},{"label":"th","span":{"begin":1470,"end":1477}},{"label":"tr","span":{"begin":1478,"end":1527}},{"label":"td","span":{"begin":1478,"end":1490}},{"label":"td","span":{"begin":1491,"end":1502}},{"label":"td","span":{"begin":1503,"end":1514}},{"label":"td","span":{"begin":1515,"end":1520}},{"label":"td","span":{"begin":1521,"end":1527}},{"label":"tr","span":{"begin":1528,"end":1591}},{"label":"td","span":{"begin":1528,"end":1554}},{"label":"td","span":{"begin":1555,"end":1566}},{"label":"td","span":{"begin":1567,"end":1578}},{"label":"td","span":{"begin":1579,"end":1584}},{"label":"td","span":{"begin":1585,"end":1591}},{"label":"tr","span":{"begin":1592,"end":1651}},{"label":"td","span":{"begin":1592,"end":1614}},{"label":"td","span":{"begin":1615,"end":1626}},{"label":"td","span":{"begin":1627,"end":1638}},{"label":"td","span":{"begin":1639,"end":1644}},{"label":"td","span":{"begin":1645,"end":1651}},{"label":"tr","span":{"begin":1652,"end":1711}},{"label":"td","span":{"begin":1652,"end":1674}},{"label":"td","span":{"begin":1675,"end":1686}},{"label":"td","span":{"begin":1687,"end":1698}},{"label":"td","span":{"begin":1699,"end":1704}},{"label":"td","span":{"begin":1705,"end":1711}},{"label":"tr","span":{"begin":1712,"end":1773}},{"label":"td","span":{"begin":1712,"end":1736}},{"label":"td","span":{"begin":1737,"end":1748}},{"label":"td","span":{"begin":1749,"end":1760}},{"label":"td","span":{"begin":1761,"end":1766}},{"label":"td","span":{"begin":1767,"end":1773}},{"label":"tr","span":{"begin":1774,"end":1818}},{"label":"td","span":{"begin":1774,"end":1789}},{"label":"td","span":{"begin":1790,"end":1797}},{"label":"td","span":{"begin":1798,"end":1805}},{"label":"td","span":{"begin":1806,"end":1811}},{"label":"td","span":{"begin":1812,"end":1818}},{"label":"tr","span":{"begin":1819,"end":1872}},{"label":"td","span":{"begin":1819,"end":1843}},{"label":"td","span":{"begin":1844,"end":1855}},{"label":"td","span":{"begin":1856,"end":1867}},{"label":"td","span":{"begin":1868,"end":1869}},{"label":"td","span":{"begin":1870,"end":1872}},{"label":"tr","span":{"begin":1873,"end":1928}},{"label":"td","span":{"begin":1873,"end":1899}},{"label":"td","span":{"begin":1900,"end":1911}},{"label":"td","span":{"begin":1912,"end":1923}},{"label":"td","span":{"begin":1924,"end":1925}},{"label":"td","span":{"begin":1926,"end":1928}},{"label":"tr","span":{"begin":1929,"end":1973}},{"label":"td","span":{"begin":1929,"end":1942}},{"label":"td","span":{"begin":1943,"end":1951}},{"label":"td","span":{"begin":1952,"end":1960}},{"label":"td","span":{"begin":1961,"end":1966}},{"label":"td","span":{"begin":1967,"end":1973}},{"label":"table-wrap-foot","span":{"begin":1974,"end":2238}},{"label":"footnote","span":{"begin":1974,"end":2238}},{"label":"p","span":{"begin":1974,"end":2238}},{"label":"title","span":{"begin":2240,"end":2258}},{"label":"p","span":{"begin":2259,"end":2606}},{"label":"figure","span":{"begin":2607,"end":3169}},{"label":"label","span":{"begin":2607,"end":2615}},{"label":"caption","span":{"begin":2616,"end":3169}},{"label":"p","span":{"begin":2616,"end":3169}}],"tracks":[{"project":"2_test","denotations":[{"id":"21994427-18786482-25127314","span":{"begin":63,"end":65},"obj":"18786482"},{"id":"21994427-18786482-25127315","span":{"begin":474,"end":476},"obj":"18786482"}],"attributes":[{"subj":"21994427-18786482-25127314","pred":"source","obj":"2_test"},{"subj":"21994427-18786482-25127315","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#93d5ec","default":true}]}]}}