PMC:3216508 / 3966-5012 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3216508","sourcedb":"PMC","sourceid":"3216508","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3216508","text":"We adopted a two-stage approach to genotype the selected SNPs for the experimental cohort, and further validated our discovery in a validation cohort. In the first stage, we scanned a relatively large number of SNPs in a limited number of samples to identify the markers with potential interest. We then selected a small number of SNPs that showed promise, and typed them in a large number of independent samples. The two-stage scan has been adopted in many genome-wide association studies and has been shown to be effective. In this study, during the first stage, we typed 48 cases and 48 controls, which were randomly selected from the pools. We then selected 6 SNPs that met our selection criteria and typed the remaining 431 cases and 310 controls. We finally genotyped one SNP (rs2227473) in the independent validation cohort (413 cases and 241 controls) to further confirm our study. The biological functions (target gene IL-22's expression) of different genotypes under specific and nonspecific stimulations were investigated and compared.","tracks":[]}