PMC:3216508 / 21349-21989 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3216508","sourcedb":"PMC","sourceid":"3216508","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3216508","text":"SNP genotyping\nGenomic DNA was extracted with a DNA isolation kit (Qiagen Inc., Germany), following the manufacturer's instruction. SNPs were typed by a high-throughput Sequenom® genotyping platform (San Diego, CA, USA). The genotypes were determined by a Homogenous Mass EXTEND assay. The Mass ARRAY AssayDesign software was used to design allele-specific extension primers. The primers designed for each selected SNP are listed in Supplementary Table S5. The genotyping for the experimental cohort was carried out in Shenzhen, and genotyping for the validation cohort was carried out in both Shenzhen and Hong Kong, with concordance \u003e98%.","divisions":[{"label":"title","span":{"begin":0,"end":14}}],"tracks":[]}