PMC:3216505 / 1759-2337 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3216505","sourcedb":"PMC","sourceid":"3216505","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3216505","text":"In a biochemical in vitro ‘budding assay' using liposomes and density gradient centrifugation (or conventional EM for visualization) vesicles are most efficiently produced with COPII and non-hydrolysable GTP, suggesting that the activated conformation of Sar1 is sufficient for budding. However, Sar1 mutants restricted to GTP in the non-hydrolysed state block cargo transport in vivo (yeast Sar1p(H77L)3 and mammalian Sar1(H79G)4). Among possible explanations for this discrepancy is a ‘trituration' artifact caused by centrifugation steps during in vitro sample preparations5.","tracks":[]}