PMC:3191169 / 28170-29607 JSONTXT

{"project":"AnEM_full-texts","denotations":[{"id":"T1","span":{"begin":129,"end":136},"obj":"Cell"},{"id":"T2","span":{"begin":279,"end":286},"obj":"Cell"},{"id":"T3","span":{"begin":480,"end":487},"obj":"Cellular_component"},{"id":"T4","span":{"begin":532,"end":538},"obj":"Cell"},{"id":"T5","span":{"begin":635,"end":644},"obj":"Cellular_component"},{"id":"T6","span":{"begin":736,"end":747},"obj":"Cellular_component"},{"id":"T7","span":{"begin":97,"end":108},"obj":"Cellular_component"},{"id":"T8","span":{"begin":560,"end":571},"obj":"Cellular_component"},{"id":"T9","span":{"begin":810,"end":821},"obj":"Cellular_component"},{"id":"T10","span":{"begin":885,"end":896},"obj":"Cellular_component"},{"id":"T11","span":{"begin":933,"end":942},"obj":"Cellular_component"},{"id":"T12","span":{"begin":978,"end":989},"obj":"Cellular_component"},{"id":"T13","span":{"begin":1032,"end":1041},"obj":"Cellular_component"},{"id":"T14","span":{"begin":1166,"end":1177},"obj":"Cellular_component"},{"id":"T15","span":{"begin":1208,"end":1219},"obj":"Cellular_component"},{"id":"T16","span":{"begin":1191,"end":1200},"obj":"Cellular_component"},{"id":"T17","span":{"begin":1268,"end":1279},"obj":"Cellular_component"},{"id":"T18","span":{"begin":1311,"end":1322},"obj":"Cellular_component"},{"id":"T19","span":{"begin":1332,"end":1341},"obj":"Cellular_component"},{"id":"T20","span":{"begin":1383,"end":1390},"obj":"Cell"},{"id":"T21","span":{"begin":674,"end":686},"obj":"Cellular_component"},{"id":"T22","span":{"begin":1125,"end":1132},"obj":"Cellular_component"}],"text":"10.1371/journal.pone.0026089.g005 Figure 5 Constitutively-active MEK1 blocks ephrin-A5 mediated growth cone collapse.\nWild-type neurons were electroporated with vectors expressing GFP (control; A, C, E) or expressing a FLAG-tagged constitutively-active MEK1 (CA-MEK1; B, D, F). Neurons were treated with ephrin-A5-Fc for 30 min followed by visualization of GFP (A, C, E) or CA-MEK-1 (green in B, D, F), F-actin and ßIII tubulin. (A, B) Overexpression of CA-MEK1 (B) reduces mean neurite length compared to a control GFP-expressing neuron (A). (C-F) A control growth cone without ephrin-A5 application (C) typically protrudes multiple filopodia (arrow). CA-MEK-1 expressing growth cones (D) were indistinguishable from a GFP-expressing growth cone. Note that CA-MEK1 was expressed in a dot-like pattern in the growth cone (arrowheads in D and F). Upon ephrin-A5 application, a control growth cone (E) collapsed resulting in only few filopodia. In contrast, a CA-MEK1 expressing growth cone (F) was only partially collapsed and many filopodia structures were preserved after ephrin-A5 induction. (G) Quantification of average neurite length. (H, I) Quantification of growth cone area (H) and filopodia number/growth cone (I) in the four conditions. Ephrin-A5 induces a growth cone collapse, resulting in reduced growth cone area and filopodia number in GFP but not CA-MEK1 expressing neurons. Scale-bar (A, B)  =  10 µm; (C-F)  =  2 µm.\n1"}