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PMC:3148254 / 4911-8813 JSONTXT

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bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue Negation Speculation
T2812 14-18 Protein denotes HOIP
T2813 19-28 Negative_regulation denotes deficient
T2814 50-60 Negative_regulation denotes disruption
T2815 64-69 Protein denotes Rnf31
T2816 89-93 Protein denotes HOIP
T2817 138-142 Protein denotes HOIP
T2818 146-155 Binding denotes recruited
T2819 291-302 Binding denotes interaction
T2820 306-310 Protein denotes CD40
T2821 316-320 Protein denotes HOIP
T2822 415-426 Binding denotes recruitment
T2823 430-434 Protein denotes HOIP
T2824 438-442 Protein denotes CD40
T2825 447-452 Protein denotes TRAF2
T2826 453-462 Regulation denotes dependent
T2827 502-506 Protein denotes HOIP
T2828 534-538 Protein denotes CD40
T2829 608-612 Protein denotes HOIP
T2830 629-633 Protein denotes CD40
T2831 713-717 Protein denotes HOIP
T2832 721-725 Protein denotes CD40
T2833 776-783 Negative_regulation denotes disrupt
T2834 802-806 Protein denotes HOIP
T2835 911-915 Protein denotes CD40
T2836 1122-1127 Protein denotes Rnf31
T2837 1147-1151 Protein denotes HOIP
T2838 1377-1386 Negative_regulation denotes disrupted
T2839 1387-1392 Protein denotes Rnf31
T2840 1456-1465 Negative_regulation denotes disrupted
T2841 1466-1471 Protein denotes Rnf31
T2842 1560-1575 Protein denotes Cre recombinase
T2843 1698-1703 Protein denotes Rnf31
T2844 1709-1718 Negative_regulation denotes disrupted
T2845 1788-1792 Protein denotes HOIP
T2846 1801-1811 Gene_expression denotes expression true true
T2847 2050-2054 Protein denotes HOIP
T2848 2055-2064 Negative_regulation denotes deficient
T2849 3429-3433 Protein denotes HOIP
T2850 3434-3443 Negative_regulation denotes deficient
T2851 3462-3472 Negative_regulation denotes disruption
T2852 3480-3485 Protein denotes Rnf31
T2853 3497-3501 Protein denotes HOIP
T2854 3502-3511 Negative_regulation denotes deficient
T2855 3588-3592 Protein denotes HOIP
T2856 3599-3608 Positive_regulation denotes restoring
T2857 3609-3613 Protein denotes HOIP
T2858 3622-3632 Gene_expression denotes expression
T2859 3675-3679 Protein denotes HOIP
T2860 3680-3693 Positive_regulation denotes reconstituted
T2861 3801-3805 Protein denotes HOIP
T2862 3806-3815 Negative_regulation denotes deficient
T14778 2116-2120 Protein denotes HOIP
T14779 2152-2157 Protein denotes Rnf31
T14780 2193-2197 Protein denotes HOIP
T14781 2434-2439 Protein denotes Rnf31
T14782 2570-2574 Protein denotes HOIP
T14783 3007-3011 Protein denotes HOIP
T14784 3012-3021 Negative_regulation denotes deficient
T14785 3023-3027 Protein denotes HOIP
T14786 3050-3058 Negative_regulation denotes decrease
T14787 3062-3066 Protein denotes HOIP
T14788 3075-3085 Gene_expression denotes expression
T14789 3106-3115 Positive_regulation denotes following
T14790 3116-3126 Negative_regulation denotes disruption
T14791 3142-3147 Protein denotes Rnf31
T14792 3149-3153 Protein denotes HOIP
T14793 3159-3163 Protein denotes HOIP
T14794 3164-3174 Gene_expression denotes expression
T14795 3282-3286 Protein denotes HOIP
T14796 3334-3338 Protein denotes HOIP
R2101 T2812 T2813 themeOf HOIP,deficient
R2102 T2815 T2814 themeOf Rnf31,disruption
R2103 T2816 T2815 equivalentTo HOIP,Rnf31
R2104 T2817 T2818 themeOf HOIP,recruited
R2105 T2820 T2819 themeOf CD40,interaction
R2106 T2821 T2819 themeOf HOIP,interaction
R2107 T2822 T2826 themeOf recruitment,dependent
R2108 T2823 T2822 themeOf HOIP,recruitment
R2109 T2824 T2822 themeOf CD40,recruitment
R2110 T2825 T2826 causeOf TRAF2,dependent
R2111 T2834 T2833 themeOf HOIP,disrupt
R2112 T2837 T2836 equivalentTo HOIP,Rnf31
R2113 T2839 T2838 themeOf Rnf31,disrupted
R2114 T2841 T2840 themeOf Rnf31,disrupted
R2115 T2843 T2844 themeOf Rnf31,disrupted
R2116 T2845 T2846 themeOf HOIP,expression
R2117 T2847 T2848 themeOf HOIP,deficient
R2118 T2849 T2850 themeOf HOIP,deficient
R2119 T2852 T2851 themeOf Rnf31,disruption
R2120 T2853 T2854 themeOf HOIP,deficient
R2121 T2857 T2858 themeOf HOIP,expression
R2122 T2858 T2856 themeOf expression,restoring
R2123 T2859 T2860 themeOf HOIP,reconstituted
R2124 T2861 T2862 themeOf HOIP,deficient
R11699 T14788 T14786 themeOf expression,decrease
R11700 T14790 T14789 causeOf disruption,following
R11701 T14791 T14790 themeOf Rnf31,disruption
R11702 T14793 T14794 themeOf HOIP,expression
R11696 T14783 T14784 themeOf HOIP,deficient
R11697 T14786 T14789 themeOf decrease,following
R11698 T14787 T14788 themeOf HOIP,expression

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T3953 64-69 Protein denotes Rnf31
T3954 50-60 Transcription denotes disruption
T3955 163-167 Protein denotes CD40
T3956 306-310 Protein denotes CD40
T3957 291-302 Binding denotes interaction
T3958 430-434 Protein denotes HOIP
T3959 438-442 Protein denotes CD40
T3960 447-452 Protein denotes TRAF2
T3961 502-513 Protein denotes HOIP mutant
T3962 534-538 Protein denotes CD40
T3963 548-553 Protein denotes NF-κB
T3964 415-426 Binding denotes recruitment
T3965 472-486 Gene_expression denotes overexpression
T3966 472-486 Positive_regulation denotes overexpression
T3967 554-564 Positive_regulation denotes activation
T3968 629-633 Protein denotes CD40
T3969 721-725 Protein denotes CD40
T3970 911-915 Protein denotes CD40
T3971 1122-1127 Protein denotes Rnf31
T3972 1387-1392 Protein denotes Rnf31
T3973 1466-1471 Protein denotes Rnf31
T3974 1560-1575 Protein denotes Cre recombinase
T3975 1698-1703 Protein denotes Rnf31
T3976 1788-1800 Protein denotes HOIP protein
T3977 1801-1811 Gene_expression denotes expression
T3978 1816-1828 Gene_expression denotes undetectable
T3979 3480-3490 Protein denotes Rnf31 gene
T3980 3609-3621 Protein denotes HOIP protein
T3981 3462-3472 Negative_regulation denotes disruption
T3982 3622-3632 Gene_expression denotes expression
T15300 2152-2157 Protein denotes Rnf31
T15301 2193-2202 Protein denotes HOIP gene
T15302 2434-2439 Protein denotes Rnf31
T15303 2511-2538 Protein denotes neomycin phosphotransferase
T15304 2540-2544 Protein denotes NeoR
T15305 2498-2507 Localization denotes insertion
T15306 2498-2507 Localization denotes insertion
T15307 2773-2776 Protein denotes Cre
T15308 2802-2822 Protein denotes NeoR coding sequence
T15309 2786-2794 Negative_regulation denotes deletion
T15310 2763-2768 Negative_regulation denotes allow
T15311 2777-2785 Positive_regulation denotes mediated
T15312 2912-2925 Protein denotes NeoR sequence
T15313 2896-2904 Negative_regulation denotes deletion
T15314 3062-3074 Protein denotes HOIP protein
T15315 3142-3147 Protein denotes Rnf31
T15316 3075-3085 Gene_expression denotes expression
T15317 3050-3058 Negative_regulation denotes decrease
T15318 3282-3286 Protein denotes HOIP
R3082 T3953 T3954 themeOf Rnf31,disruption
R3087 T3956 T3957 themeOf CD40,interaction
R3088 T3958 T3964 themeOf HOIP,recruitment
R3089 T3959 T3964 themeOf CD40,recruitment
R3090 T3961 T3965 themeOf HOIP mutant,overexpression
R3091 T3963 T3967 themeOf NF-κB,activation
R3092 T3965 T3966 themeOf overexpression,overexpression
R3093 T3976 T3977 themeOf HOIP protein,expression
R3094 T3979 T3981 themeOf Rnf31 gene,disruption
R3095 T3980 T3982 themeOf HOIP protein,expression
R3096 T3976 T3978 themeOf HOIP protein,undetectable
R12133 T15303 T15305 themeOf neomycin phosphotransferase,insertion
R12134 T15304 T15306 themeOf NeoR,insertion
R12135 T15307 T15311 causeOf Cre,mediated
R12136 T15308 T15309 themeOf NeoR coding sequence,deletion
R12137 T15309 T15310 themeOf deletion,allow
R12138 T15309 T15311 themeOf deletion,mediated
R12139 T15312 T15313 themeOf NeoR sequence,deletion
R12140 T15314 T15316 themeOf HOIP protein,expression
R12141 T15316 T15317 themeOf expression,decrease

events-check-again

Id Subject Object Predicate Lexical cue Negation Speculation
T3983 14-18 Protein denotes HOIP
T3984 19-28 Negative_regulation denotes deficient
T3985 50-60 Negative_regulation denotes disruption
T3986 64-69 Protein denotes Rnf31
T3987 89-93 Protein denotes HOIP
T3988 138-142 Protein denotes HOIP
T3989 146-155 Binding denotes recruited
T3990 291-302 Binding denotes interaction
T3991 306-310 Protein denotes CD40
T3992 316-320 Protein denotes HOIP
T3993 415-426 Binding denotes recruitment
T3994 430-434 Protein denotes HOIP
T3995 438-442 Protein denotes CD40
T3996 447-452 Protein denotes TRAF2
T3997 453-462 Regulation denotes dependent
T3998 502-506 Protein denotes HOIP
T3999 534-538 Protein denotes CD40
T4000 608-612 Protein denotes HOIP
T4001 629-633 Protein denotes CD40
T4002 713-717 Protein denotes HOIP
T4003 721-725 Protein denotes CD40
T4004 776-783 Negative_regulation denotes disrupt
T4005 802-806 Protein denotes HOIP
T4006 911-915 Protein denotes CD40
T4007 1122-1127 Protein denotes Rnf31
T4008 1147-1151 Protein denotes HOIP
T4009 1377-1386 Negative_regulation denotes disrupted
T4010 1387-1392 Protein denotes Rnf31
T4011 1456-1465 Negative_regulation denotes disrupted
T4012 1466-1471 Protein denotes Rnf31
T4013 1560-1575 Protein denotes Cre recombinase
T4014 1698-1703 Protein denotes Rnf31
T4015 1709-1718 Negative_regulation denotes disrupted
T4016 1788-1792 Protein denotes HOIP
T4017 1801-1811 Gene_expression denotes expression true true
T4018 2050-2054 Protein denotes HOIP
T4019 2055-2064 Negative_regulation denotes deficient
T4020 3429-3433 Protein denotes HOIP
T4021 3434-3443 Negative_regulation denotes deficient
T4022 3462-3472 Negative_regulation denotes disruption
T4023 3480-3485 Protein denotes Rnf31
T4024 3497-3501 Protein denotes HOIP
T4025 3502-3511 Negative_regulation denotes deficient
T4026 3588-3592 Protein denotes HOIP
T4027 3599-3608 Positive_regulation denotes restoring
T4028 3609-3613 Protein denotes HOIP
T4029 3622-3632 Gene_expression denotes expression
T4030 3675-3679 Protein denotes HOIP
T4031 3680-3693 Positive_regulation denotes reconstituted
T4032 3801-3805 Protein denotes HOIP
T4033 3806-3815 Negative_regulation denotes deficient
T15319 2116-2120 Protein denotes HOIP
T15320 2152-2157 Protein denotes Rnf31
T15321 2193-2197 Protein denotes HOIP
T15322 2434-2439 Protein denotes Rnf31
T15323 2570-2574 Protein denotes HOIP
T15324 3007-3011 Protein denotes HOIP
T15325 3012-3021 Negative_regulation denotes deficient
T15326 3023-3027 Protein denotes HOIP
T15327 3050-3058 Negative_regulation denotes decrease
T15328 3062-3066 Protein denotes HOIP
T15329 3075-3085 Gene_expression denotes expression
T15330 3106-3115 Positive_regulation denotes following
T15331 3116-3126 Negative_regulation denotes disruption
T15332 3142-3147 Protein denotes Rnf31
T15333 3149-3153 Protein denotes HOIP
T15334 3159-3163 Protein denotes HOIP
T15335 3164-3174 Gene_expression denotes expression
T15336 3282-3286 Protein denotes HOIP
T15337 3334-3338 Protein denotes HOIP
R3097 T4012 T4011 themeOf Rnf31,disrupted
R3098 T4014 T4015 themeOf Rnf31,disrupted
R3099 T4016 T4017 themeOf HOIP,expression
R3100 T3983 T3984 themeOf HOIP,deficient
R3101 T4018 T4019 themeOf HOIP,deficient
R3102 T3986 T3985 themeOf Rnf31,disruption
R3103 T4020 T4021 themeOf HOIP,deficient
R3104 T3987 T3986 equivalentTo HOIP,Rnf31
R3105 T3988 T3989 themeOf HOIP,recruited
R3106 T4023 T4022 themeOf Rnf31,disruption
R3107 T3991 T3990 themeOf CD40,interaction
R3108 T3992 T3990 themeOf HOIP,interaction
R3109 T4024 T4025 themeOf HOIP,deficient
R3110 T3993 T3997 themeOf recruitment,dependent
R3111 T3994 T3993 themeOf HOIP,recruitment
R3112 T3995 T3993 themeOf CD40,recruitment
R3113 T3996 T3997 causeOf TRAF2,dependent
R3114 T4028 T4029 themeOf HOIP,expression
R3115 T4029 T4027 themeOf expression,restoring
R3116 T4030 T4031 themeOf HOIP,reconstituted
R3117 T4005 T4004 themeOf HOIP,disrupt
R3118 T4008 T4007 equivalentTo HOIP,Rnf31
R3119 T4010 T4009 themeOf Rnf31,disrupted
R3120 T4032 T4033 themeOf HOIP,deficient
R12142 T15324 T15325 themeOf HOIP,deficient
R12143 T15327 T15330 themeOf decrease,following
R12144 T15328 T15329 themeOf HOIP,expression
R12145 T15329 T15327 themeOf expression,decrease
R12146 T15331 T15330 causeOf disruption,following
R12147 T15332 T15331 themeOf Rnf31,disruption
R12148 T15334 T15335 themeOf HOIP,expression

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T3341 0-10 NN denotes Generation
T3342 11-13 IN denotes of
T3343 14-28 JJ denotes HOIP-deficient
T3344 29-30 NN denotes B
T3345 31-36 NN denotes cells
T3346 37-40 IN denotes via
T3347 41-49 VB denotes targeted
T3348 50-60 NN denotes disruption
T3349 61-63 IN denotes of
T3350 64-69 NN denotes Rnf31
T3351 69-70 -COMMA- denotes ,
T3352 71-74 DT denotes the
T3353 75-79 NN denotes gene
T3354 80-88 VB denotes encoding
T3355 89-93 NN denotes HOIP
T3356 94-96 IN denotes In
T3357 97-100 PRP-DOLLAR- denotes our
T3358 101-109 JJ denotes previous
T3359 110-115 NN denotes study
T3360 115-116 -COMMA- denotes ,
T3361 117-119 PRP denotes we
T3362 120-132 VB denotes demonstrated
T3363 133-137 IN denotes that
T3364 138-142 NN denotes HOIP
T3365 143-145 VB denotes is
T3366 146-155 VB denotes recruited
T3367 156-158 TO denotes to
T3368 159-162 DT denotes the
T3369 163-167 NN denotes CD40
T3370 168-177 NN denotes signaling
T3371 178-185 NN denotes complex
T3372 186-188 IN denotes in
T3373 189-192 CD denotes two
T3374 193-198 NN denotes mouse
T3375 199-200 NN denotes B
T3376 201-205 NN denotes cell
T3377 206-211 NN denotes lines
T3378 212-213 -LRB- denotes [
T3379 213-214 CD denotes 6
T3380 214-215 -RRB- denotes ]
T3381 217-224 JJ denotes Similar
T3382 225-232 NN denotes results
T3383 233-237 VB denotes were
T3384 238-246 VB denotes obtained
T3385 247-251 IN denotes with
T3386 252-257 NN denotes mouse
T3387 258-269 NN denotes splenocytes
T3388 269-270 -COMMA- denotes ,
T3389 271-281 VB denotes indicating
T3390 282-286 IN denotes that
T3391 287-290 DT denotes the
T3392 291-302 NN denotes interaction
T3393 303-305 IN denotes of
T3394 306-310 NN denotes CD40
T3395 311-315 IN denotes with
T3396 316-320 NN denotes HOIP
T3397 321-323 VB denotes is
T3398 324-327 RB denotes not
T3399 328-335 VB denotes limited
T3400 336-338 TO denotes to
T3401 339-350 VB denotes transformed
T3402 351-352 NN denotes B
T3403 353-357 NN denotes cell
T3404 358-363 NN denotes lines
T3405 364-365 -LRB- denotes (
T3406 365-376 JJ denotes unpublished
T3407 377-389 NN denotes observations
T3408 389-390 -RRB- denotes )
T3409 392-394 PRP denotes We
T3410 395-399 RB denotes also
T3411 400-405 VB denotes found
T3412 406-410 IN denotes that
T3413 411-414 DT denotes the
T3414 415-426 NN denotes recruitment
T3415 427-429 IN denotes of
T3416 430-434 NN denotes HOIP
T3417 435-437 TO denotes to
T3418 438-442 NN denotes CD40
T3419 443-446 VB denotes was
T3420 447-462 JJ denotes TRAF2-dependent
T3421 463-466 CC denotes and
T3422 467-471 IN denotes that
T3423 472-486 NN denotes overexpression
T3424 487-489 IN denotes of
T3425 490-491 DT denotes a
T3426 492-501 VB denotes truncated
T3427 502-506 NN denotes HOIP
T3428 507-513 NN denotes mutant
T3429 514-523 RB denotes partially
T3430 524-533 VB denotes inhibited
T3431 534-547 JJ denotes CD40-mediated
T3432 548-553 NN denotes NF-κB
T3433 554-564 NN denotes activation
T3434 566-571 DT denotes These
T3435 572-579 NN denotes results
T3436 580-587 VB denotes support
T3437 588-591 DT denotes the
T3438 592-602 NN denotes hypothesis
T3439 603-607 IN denotes that
T3440 608-612 NN denotes HOIP
T3441 613-618 VB denotes plays
T3442 619-620 DT denotes a
T3443 621-625 NN denotes role
T3444 626-628 IN denotes in
T3445 629-633 NN denotes CD40
T3446 634-640 NN denotes signal
T3447 641-653 NN denotes transduction
T3448 655-657 TO denotes To
T3449 658-665 RB denotes further
T3450 666-672 VB denotes define
T3451 673-676 DT denotes the
T3452 677-681 NN denotes role
T3453 682-685 CC denotes and
T3454 686-694 VB denotes evaluate
T3455 695-698 DT denotes the
T3456 699-709 NN denotes importance
T3457 710-712 IN denotes of
T3458 713-717 NN denotes HOIP
T3459 718-720 IN denotes in
T3460 721-725 NN denotes CD40
T3461 726-735 NN denotes signaling
T3462 735-736 -COMMA- denotes ,
T3463 737-739 PRP denotes we
T3464 740-744 VB denotes used
T3465 745-752 JJ denotes somatic
T3466 753-757 NN denotes cell
T3467 758-762 NN denotes gene
T3468 763-772 VB denotes targeting
T3469 773-775 TO denotes to
T3470 776-783 VB denotes disrupt
T3471 784-787 DT denotes the
T3472 788-792 NN denotes gene
T3473 793-801 VB denotes encoding
T3474 802-806 NN denotes HOIP
T3475 807-809 IN denotes in
T3476 810-813 DT denotes the
T3477 814-819 NN denotes mouse
T3478 820-821 NN denotes B
T3479 822-826 NN denotes cell
T3480 827-831 NN denotes line
T3481 832-838 NN denotes A20.2J
T3482 840-844 DT denotes This
T3483 845-849 NN denotes cell
T3484 850-854 NN denotes line
T3485 855-858 VB denotes has
T3486 859-863 VB denotes been
T3487 864-876 RB denotes particularly
T3488 877-883 JJ denotes useful
T3489 884-886 IN denotes in
T3490 887-890 DT denotes the
T3491 891-907 NN denotes characterization
T3492 908-910 IN denotes of
T3493 911-915 NN denotes CD40
T3494 916-925 NN denotes signaling
T3495 926-936 NN denotes mechanisms
T3496 937-940 JJ denotes due
T3497 941-943 TO denotes to
T3498 944-947 DT denotes the
T3499 948-956 JJ denotes relative
T3500 957-961 NN denotes ease
T3501 962-966 IN denotes with
T3502 967-972 WDT denotes which
T3503 973-976 PRP-DOLLAR- denotes its
T3504 977-983 NN denotes genome
T3505 984-987 MD denotes can
T3506 988-990 VB denotes be
T3507 991-999 VB denotes modified
T3508 1000-1002 IN denotes by
T3509 1003-1013 JJ denotes homologous
T3510 1014-1027 NN denotes recombination
T3511 1028-1029 -LRB- denotes [
T3512 1029-1030 CD denotes 7
T3513 1030-1031 -RRB- denotes ]
T3514 1031-1032 -COMMA- denotes ,
T3515 1033-1034 -LRB- denotes [
T3516 1034-1035 CD denotes 8
T3517 1035-1036 -RRB- denotes ]
T3518 1036-1037 -COMMA- denotes ,
T3519 1038-1039 -LRB- denotes [
T3520 1039-1040 CD denotes 9
T3521 1040-1041 -RRB- denotes ]
T3522 1043-1045 PRP denotes We
T3523 1046-1050 VB denotes used
T3524 1051-1052 DT denotes a
T3525 1053-1062 VB denotes targeting
T3526 1063-1069 NN denotes vector
T3527 1070-1077 JJ denotes capable
T3528 1078-1080 IN denotes of
T3529 1081-1091 VB denotes undergoing
T3530 1092-1102 JJ denotes homologous
T3531 1103-1116 NN denotes recombination
T3532 1117-1121 IN denotes with
T3533 1122-1127 NN denotes Rnf31
T3534 1128-1129 -LRB- denotes (
T3535 1129-1132 DT denotes the
T3536 1133-1137 NN denotes gene
T3537 1138-1146 VB denotes encoding
T3538 1147-1151 NN denotes HOIP
T3539 1151-1152 -RRB- denotes )
T3540 1153-1155 TO denotes to
T3541 1156-1163 VB denotes disrupt
T3542 1164-1167 DT denotes the
T3543 1168-1174 VB denotes coding
T3544 1175-1183 NN denotes sequence
T3545 1184-1186 IN denotes of
T3546 1187-1190 DT denotes the
T3547 1191-1195 NN denotes gene
T3548 1196-1198 IN denotes in
T3549 1199-1203 NN denotes exon
T3550 1204-1205 CD denotes 5
T3551 1206-1207 -LRB- denotes (
T3552 1207-1211 NNP denotes Fig.
T3553 1212-1214 NN denotes 1A
T3554 1214-1215 -RRB- denotes )
T3555 1217-1226 VB denotes Following
T3556 1227-1239 NN denotes introduction
T3557 1240-1242 IN denotes of
T3558 1243-1246 DT denotes the
T3559 1247-1253 NN denotes vector
T3560 1253-1254 -COMMA- denotes ,
T3561 1255-1258 DT denotes the
T3562 1259-1277 JJ denotes neomycin-resistant
T3563 1278-1284 NN denotes clones
T3564 1285-1289 WDT denotes that
T3565 1290-1295 VB denotes arose
T3566 1296-1300 VB denotes were
T3567 1301-1309 VB denotes screened
T3568 1310-1312 IN denotes by
T3569 1313-1316 NN denotes PCR
T3570 1317-1330 NN denotes amplification
T3571 1331-1333 IN denotes of
T3572 1334-1341 JJ denotes genomic
T3573 1342-1345 NN denotes DNA
T3574 1346-1348 TO denotes to
T3575 1349-1357 VB denotes identify
T3576 1358-1363 NN denotes cells
T3577 1364-1374 VB denotes containing
T3578 1375-1376 DT denotes a
T3579 1377-1386 VB denotes disrupted
T3580 1387-1392 NN denotes Rnf31
T3581 1393-1399 NN denotes allele
T3582 1401-1403 TO denotes To
T3583 1404-1410 VB denotes remove
T3584 1411-1414 DT denotes the
T3585 1415-1425 JJ denotes selectable
T3586 1426-1432 NN denotes marker
T3587 1433-1437 NN denotes gene
T3588 1438-1446 NN denotes cassette
T3589 1447-1451 IN denotes from
T3590 1452-1455 DT denotes the
T3591 1456-1465 VB denotes disrupted
T3592 1466-1471 NN denotes Rnf31
T3593 1472-1478 NN denotes allele
T3594 1478-1479 -COMMA- denotes ,
T3595 1480-1491 JJ denotes recombinant
T3596 1492-1496 NN denotes cell
T3597 1497-1502 NN denotes lines
T3598 1503-1507 VB denotes were
T3599 1508-1519 RB denotes transiently
T3600 1520-1531 VB denotes transfected
T3601 1532-1536 IN denotes with
T3602 1537-1538 DT denotes a
T3603 1539-1546 NN denotes plasmid
T3604 1547-1551 WDT denotes that
T3605 1552-1559 VB denotes encodes
T3606 1560-1563 NNP denotes Cre
T3607 1564-1575 NN denotes recombinase
T3608 1577-1581 DT denotes This
T3609 1582-1586 NN denotes step
T3610 1587-1594 VB denotes allowed
T3611 1595-1597 PRP denotes us
T3612 1598-1600 TO denotes to
T3613 1601-1608 VB denotes perform
T3614 1609-1610 DT denotes a
T3615 1611-1617 JJ denotes second
T3616 1618-1623 NN denotes round
T3617 1624-1626 IN denotes of
T3618 1627-1636 VB denotes targeting
T3619 1637-1640 CC denotes and
T3620 1641-1645 NN denotes drug
T3621 1646-1655 NN denotes selection
T3622 1655-1656 -COMMA- denotes ,
T3623 1657-1667 VB denotes generating
T3624 1668-1673 NN denotes cells
T3625 1674-1676 IN denotes in
T3626 1677-1682 WDT denotes which
T3627 1683-1687 DT denotes both
T3628 1688-1694 NN denotes copies
T3629 1695-1697 IN denotes of
T3630 1698-1703 NN denotes Rnf31
T3631 1704-1708 VB denotes were
T3632 1709-1718 VB denotes disrupted
T3633 1720-1723 CD denotes Two
T3634 1724-1735 JJ denotes independent
T3635 1736-1742 JJ denotes clonal
T3636 1743-1747 NN denotes cell
T3637 1748-1753 NN denotes lines
T3638 1754-1758 VB denotes were
T3639 1759-1765 VB denotes chosen
T3640 1766-1769 IN denotes for
T3641 1770-1777 JJ denotes further
T3642 1778-1786 NN denotes analysis
T3643 1788-1792 NN denotes HOIP
T3644 1793-1800 NN denotes protein
T3645 1801-1811 NN denotes expression
T3646 1812-1815 VB denotes was
T3647 1816-1828 JJ denotes undetectable
T3648 1829-1831 IN denotes in
T3649 1832-1836 DT denotes both
T3650 1837-1841 NN denotes cell
T3651 1842-1847 NN denotes lines
T3652 1847-1848 -COMMA- denotes ,
T3653 1849-1851 IN denotes as
T3654 1852-1862 VB denotes determined
T3655 1863-1865 IN denotes by
T3656 1866-1873 JJ denotes Western
T3657 1874-1878 NN denotes blot
T3658 1879-1887 NN denotes analysis
T3659 1888-1890 IN denotes of
T3660 1891-1895 NN denotes cell
T3661 1896-1903 NN denotes lysates
T3662 1904-1905 -LRB- denotes (
T3663 1905-1909 NNP denotes Fig.
T3664 1910-1912 NN denotes 1B
T3665 1912-1913 -RRB- denotes )
T3666 1913-1914 -COMMA- denotes ,
T3667 1915-1928 VB denotes demonstrating
T3668 1929-1933 IN denotes that
T3669 1934-1937 DT denotes the
T3670 1938-1947 VB denotes targeting
T3671 1948-1955 NN denotes process
T3672 1956-1959 VB denotes was
T3673 1960-1970 JJ denotes successful
T3674 1972-1974 IN denotes In
T3675 1975-1978 DT denotes the
T3676 1979-1983 NN denotes text
T3677 1984-1988 WDT denotes that
T3678 1989-1996 VB denotes follows
T3679 1996-1997 -COMMA- denotes ,
T3680 1998-2001 DT denotes the
T3681 2002-2005 CD denotes two
T3682 2006-2019 JJ denotes gene-targeted
T3683 2020-2024 NN denotes cell
T3684 2025-2030 NN denotes lines
T3685 2031-2034 VB denotes are
T3686 2035-2043 VB denotes referred
T3687 2044-2046 TO denotes to
T3688 2047-2049 IN denotes as
T3689 2050-2064 JJ denotes HOIP-deficient
T3690 2065-2070 NN denotes cells
T3691 3352-3354 TO denotes To
T3692 3355-3361 VB denotes enable
T3693 3362-3364 PRP denotes us
T3694 3365-3367 TO denotes to
T3695 3368-3375 VB denotes confirm
T3696 3376-3380 IN denotes that
T3697 3381-3384 DT denotes any
T3698 3385-3394 NN denotes signaling
T3699 3395-3397 CC denotes or
T3700 3398-3408 JJ denotes functional
T3701 3409-3416 NN denotes defects
T3702 3417-3425 VB denotes observed
T3703 3426-3428 IN denotes in
T3704 3429-3443 JJ denotes HOIP-deficient
T3705 3444-3449 NN denotes cells
T3706 3450-3454 VB denotes were
T3707 3455-3458 JJ denotes due
T3708 3459-3461 TO denotes to
T3709 3462-3472 NN denotes disruption
T3710 3473-3475 IN denotes of
T3711 3476-3479 DT denotes the
T3712 3480-3485 NN denotes Rnf31
T3713 3486-3490 NN denotes gene
T3714 3490-3491 -COMMA- denotes ,
T3715 3492-3496 DT denotes both
T3716 3497-3511 JJ denotes HOIP-deficient
T3717 3512-3516 NN denotes cell
T3718 3517-3522 NN denotes lines
T3719 3523-3527 VB denotes were
T3720 3528-3538 VB denotes transduced
T3721 3539-3543 IN denotes with
T3722 3544-3545 DT denotes a
T3723 3546-3556 NN denotes retrovirus
T3724 3557-3565 VB denotes encoding
T3725 3566-3577 JJ denotes FLAG-tagged
T3726 3578-3587 JJ denotes wild-type
T3727 3588-3592 NN denotes HOIP
T3728 3592-3593 -COMMA- denotes ,
T3729 3594-3598 RB denotes thus
T3730 3599-3608 VB denotes restoring
T3731 3609-3613 NN denotes HOIP
T3732 3614-3621 NN denotes protein
T3733 3622-3632 NN denotes expression
T3734 3633-3634 -LRB- denotes (
T3735 3634-3638 NN denotes Fig.
T3736 3639-3641 NN denotes 1B
T3737 3641-3642 -RRB- denotes )
T3738 3644-3649 DT denotes These
T3739 3650-3655 NN denotes cells
T3740 3656-3659 VB denotes are
T3741 3660-3668 VB denotes referred
T3742 3669-3671 TO denotes to
T3743 3672-3674 IN denotes as
T3744 3675-3693 JJ denotes HOIP-reconstituted
T3745 3694-3699 NN denotes cells
T3746 3701-3703 IN denotes In
T3747 3704-3707 DT denotes the
T3748 3708-3719 NN denotes experiments
T3749 3720-3724 WDT denotes that
T3750 3725-3731 VB denotes follow
T3751 3731-3732 -COMMA- denotes ,
T3752 3733-3742 NN denotes responses
T3753 3743-3745 IN denotes by
T3754 3746-3751 DT denotes these
T3755 3752-3757 NN denotes cells
T3756 3758-3762 VB denotes were
T3757 3763-3771 VB denotes compared
T3758 3772-3774 TO denotes to
T3759 3775-3780 DT denotes those
T3760 3781-3783 IN denotes of
T3761 3784-3790 NN denotes A20.2J
T3762 3791-3796 NN denotes cells
T3763 3797-3800 CC denotes and
T3764 3801-3815 JJ denotes HOIP-deficient
T3765 3816-3821 NN denotes cells
T3766 3822-3832 VB denotes transduced
T3767 3833-3837 IN denotes with
T3768 3838-3839 DT denotes a
T3769 3840-3850 JJ denotes retroviral
T3770 3851-3857 NN denotes vector
T3771 3858-3859 -LRB- denotes (
T3772 3859-3863 NN denotes pMIP
T3773 3863-3864 -RRB- denotes )
T3774 3865-3872 VB denotes lacking
T3775 3873-3874 DT denotes a
T3776 3875-3879 NN denotes cDNA
T3777 3880-3886 NN denotes insert
T3778 3887-3888 -LRB- denotes (
T3779 3888-3893 JJ denotes empty
T3780 3894-3900 NN denotes vector
T3781 3900-3901 -RRB- denotes )
T15027 2116-2120 NN denotes HOIP
T15028 2121-2125 NN denotes gene
T15029 2126-2135 NN denotes targeting
T15030 2137-2138 -LRB- denotes (
T15031 2138-2139 NN denotes A
T15032 2139-2140 -RRB- denotes )
T15033 2141-2148 NN denotes Regions
T15034 2149-2151 IN denotes of
T15035 2152-2157 NN denotes Rnf31
T15036 2158-2162 NN denotes gene
T15037 2163-2171 NN denotes sequence
T15038 2172-2173 -LRB- denotes (
T15039 2173-2179 NN denotes bottom
T15040 2179-2180 -RRB- denotes )
T15041 2181-2185 VB denotes used
T15042 2186-2188 IN denotes in
T15043 2189-2192 DT denotes the
T15044 2193-2197 NN denotes HOIP
T15045 2198-2202 NN denotes gene
T15046 2203-2212 NN denotes targeting
T15047 2213-2219 NN denotes vector
T15048 2220-2221 -LRB- denotes (
T15049 2221-2224 NN denotes top
T15050 2224-2225 -RRB- denotes )
T15051 2226-2229 VB denotes are
T15052 2230-2235 VB denotes shown
T15053 2237-2243 NN denotes Arrows
T15054 2244-2245 -LRB- denotes (
T15055 2245-2246 NN denotes F
T15056 2247-2250 CC denotes and
T15057 2251-2252 NN denotes R
T15058 2252-2253 -RRB- denotes )
T15059 2254-2262 VB denotes indicate
T15060 2263-2274 JJ denotes approximate
T15061 2275-2284 NN denotes positions
T15062 2285-2287 IN denotes of
T15063 2288-2297 NN denotes sequences
T15064 2298-2308 JJ denotes homologous
T15065 2309-2311 TO denotes to
T15066 2312-2328 NN denotes oligonucleotides
T15067 2329-2333 VB denotes used
T15068 2334-2337 IN denotes for
T15069 2338-2350 JJ denotes PCR-mediated
T15070 2351-2360 NN denotes detection
T15071 2361-2363 IN denotes of
T15072 2364-2374 JJ denotes homologous
T15073 2375-2388 NN denotes recombination
T15074 2390-2400 JJ denotes Homologous
T15075 2401-2414 NN denotes recombination
T15076 2415-2417 IN denotes of
T15077 2418-2421 DT denotes the
T15078 2422-2428 NN denotes vector
T15079 2429-2433 IN denotes with
T15080 2434-2439 NN denotes Rnf31
T15081 2440-2448 VB denotes resulted
T15082 2449-2451 IN denotes in
T15083 2452-2453 DT denotes a
T15084 2454-2459 JJ denotes small
T15085 2460-2471 JJ denotes chromosomal
T15086 2472-2480 NN denotes deletion
T15087 2481-2484 CC denotes and
T15088 2485-2488 DT denotes the
T15089 2489-2497 JJ denotes in-frame
T15090 2498-2507 NN denotes insertion
T15091 2508-2510 IN denotes of
T15092 2511-2519 NN denotes neomycin
T15093 2520-2538 NN denotes phosphotransferase
T15094 2539-2540 -LRB- denotes (
T15095 2540-2544 NN denotes NeoR
T15096 2544-2545 -RRB- denotes )
T15097 2546-2550 IN denotes into
T15098 2551-2554 DT denotes the
T15099 2555-2569 JJ denotes amino-terminal
T15100 2570-2574 NN denotes HOIP
T15101 2575-2581 VB denotes coding
T15102 2582-2590 NN denotes sequence
T15103 2592-2593 DT denotes A
T15104 2594-2604 NN denotes diphtheria
T15105 2605-2610 NN denotes toxin
T15106 2611-2612 -LRB- denotes (
T15107 2612-2614 NN denotes DT
T15108 2614-2615 -RRB- denotes )
T15109 2616-2624 NN denotes cassette
T15110 2625-2627 IN denotes in
T15111 2628-2631 DT denotes the
T15112 2632-2638 NN denotes vector
T15113 2639-2650 VB denotes facilitates
T15114 2651-2654 DT denotes the
T15115 2655-2663 JJ denotes negative
T15116 2664-2673 NN denotes selection
T15117 2674-2676 IN denotes of
T15118 2677-2682 NN denotes cells
T15119 2683-2685 IN denotes in
T15120 2686-2691 WDT denotes which
T15121 2692-2698 VB denotes random
T15122 2699-2710 JJ denotes chromosomal
T15123 2711-2720 NN denotes insertion
T15124 2721-2723 IN denotes of
T15125 2724-2727 DT denotes the
T15126 2728-2734 NN denotes vector
T15127 2735-2740 VB denotes takes
T15128 2741-2746 NN denotes place
T15129 2748-2752 NN denotes LoxP
T15130 2753-2762 NN denotes sequences
T15131 2763-2768 VB denotes allow
T15132 2769-2772 DT denotes the
T15133 2773-2785 JJ denotes Cre-mediated
T15134 2786-2794 NN denotes deletion
T15135 2795-2797 IN denotes of
T15136 2798-2801 DT denotes the
T15137 2802-2806 NN denotes NeoR
T15138 2807-2813 NN denotes coding
T15139 2814-2822 NN denotes sequence
T15140 2824-2827 DT denotes The
T15141 2828-2834 NN denotes SV40pA
T15142 2835-2843 NN denotes sequence
T15143 2844-2849 VB denotes helps
T15144 2850-2852 TO denotes to
T15145 2853-2859 VB denotes ensure
T15146 2860-2870 NN denotes disruption
T15147 2871-2873 IN denotes of
T15148 2874-2878 NN denotes gene
T15149 2879-2889 NN denotes expression
T15150 2890-2895 IN denotes after
T15151 2896-2904 NN denotes deletion
T15152 2905-2907 IN denotes of
T15153 2908-2911 DT denotes the
T15154 2912-2916 NN denotes NeoR
T15155 2917-2926 NN denotes sequence.
T15156 2927-2928 -LRB- denotes (
T15157 2928-2929 NN denotes B
T15158 2929-2930 -RRB- denotes )
T15159 2931-2940 NN denotes Anti-HOIP
T15160 2941-2944 CC denotes and
T15161 2945-2954 JJ denotes anti-FLAG
T15162 2955-2962 JJ denotes Western
T15163 2963-2968 NN denotes blots
T15164 2969-2971 IN denotes of
T15165 2972-2976 NN denotes cell
T15166 2977-2984 NN denotes lysates
T15167 2985-2989 IN denotes from
T15168 2990-2996 NN denotes A20.2J
T15169 2997-3002 NN denotes cells
T15170 3003-3006 CC denotes and
T15171 3007-3021 JJ denotes HOIP-deficient
T15172 3022-3023 -LRB- denotes (
T15173 3023-3030 JJ denotes HOIP-/-
T15174 3030-3031 -RRB- denotes )
T15175 3032-3038 NN denotes clones
T15176 3040-3041 DT denotes A
T15177 3042-3049 JJ denotes partial
T15178 3050-3058 NN denotes decrease
T15179 3059-3061 IN denotes in
T15180 3062-3066 NN denotes HOIP
T15181 3067-3074 NN denotes protein
T15182 3075-3085 NN denotes expression
T15183 3086-3088 VB denotes is
T15184 3089-3096 JJ denotes evident
T15185 3097-3099 IN denotes in
T15186 3100-3105 NN denotes cells
T15187 3106-3115 VB denotes following
T15188 3116-3126 NN denotes disruption
T15189 3127-3129 IN denotes of
T15190 3130-3133 CD denotes one
T15191 3134-3138 NN denotes copy
T15192 3139-3141 IN denotes of
T15193 3142-3147 NN denotes Rnf31
T15194 3148-3149 -LRB- denotes (
T15195 3149-3156 NN denotes HOIP−/+
T15196 3156-3157 -RRB- denotes )
T15197 3159-3163 NN denotes HOIP
T15198 3164-3174 NN denotes expression
T15199 3175-3177 IN denotes in
T15200 3178-3184 NN denotes clones
T15201 3185-3198 VB denotes reconstituted
T15202 3199-3203 IN denotes with
T15203 3204-3206 DT denotes an
T15204 3207-3212 JJ denotes empty
T15205 3213-3223 JJ denotes retroviral
T15206 3224-3230 NN denotes vector
T15207 3231-3232 -LRB- denotes (
T15208 3232-3236 NN denotes pMIP
T15209 3236-3237 -RRB- denotes )
T15210 3238-3240 CC denotes or
T15211 3241-3242 DT denotes a
T15212 3243-3253 JJ denotes retroviral
T15213 3254-3260 NN denotes vector
T15214 3261-3269 VB denotes encoding
T15215 3270-3281 JJ denotes FLAG-tagged
T15216 3282-3286 NN denotes HOIP
T15217 3287-3289 VB denotes is
T15218 3290-3294 RB denotes also
T15219 3295-3300 VB denotes shown
T15220 3302-3313 JJ denotes Approximate
T15221 3314-3323 JJ denotes molecular
T15222 3324-3330 NN denotes weight
T15223 3331-3333 IN denotes of
T15224 3334-3338 NN denotes HOIP
T15225 3339-3341 VB denotes is
T15226 3342-3345 CD denotes 120
T15227 3346-3348 NN denotes kD
R2586 T3341 T3342 arg1Of Generation,of
R2587 T3341 T3346 arg1Of Generation,via
R2588 T3345 T3342 arg2Of cells,of
R2589 T3345 T3343 arg1Of cells,HOIP-deficient
R2590 T3345 T3344 arg1Of cells,B
R2591 T3348 T3346 arg2Of disruption,via
R2592 T3348 T3347 arg2Of disruption,targeted
R2593 T3348 T3349 arg1Of disruption,of
R2594 T3350 T3349 arg2Of Rnf31,of
R2595 T3350 T3351 arg1Of Rnf31,","
R2596 T3353 T3351 arg2Of gene,","
R2597 T3353 T3352 arg1Of gene,the
R2598 T3353 T3354 arg1Of gene,encoding
R2599 T3355 T3354 arg2Of HOIP,encoding
R2600 T3359 T3356 arg2Of study,In
R2601 T3359 T3357 arg1Of study,our
R2602 T3359 T3358 arg1Of study,previous
R2603 T3361 T3362 arg1Of we,demonstrated
R2604 T3362 T3356 arg1Of demonstrated,In
R2605 T3362 T3360 arg1Of demonstrated,","
R2606 T3364 T3365 arg1Of HOIP,is
R2607 T3364 T3366 arg2Of HOIP,recruited
R2608 T3366 T3362 arg2Of recruited,demonstrated
R2609 T3366 T3363 arg1Of recruited,that
R2610 T3366 T3365 arg2Of recruited,is
R2611 T3366 T3367 arg1Of recruited,to
R2612 T3366 T3372 arg1Of recruited,in
R2613 T3366 T3378 arg1Of recruited,[
R2614 T3371 T3367 arg2Of complex,to
R2615 T3371 T3368 arg1Of complex,the
R2616 T3371 T3369 arg1Of complex,CD40
R2617 T3371 T3370 arg1Of complex,signaling
R2618 T3377 T3372 arg2Of lines,in
R2619 T3377 T3373 arg1Of lines,two
R2620 T3377 T3374 arg1Of lines,mouse
R2621 T3377 T3375 arg1Of lines,B
R2622 T3377 T3376 arg1Of lines,cell
R2623 T3379 T3378 arg2Of 6,[
R2624 T3380 T3378 arg3Of ],[
R2625 T3382 T3381 arg1Of results,Similar
R2626 T3382 T3383 arg1Of results,were
R2627 T3382 T3384 arg2Of results,obtained
R2628 T3384 T3383 arg2Of obtained,were
R2629 T3384 T3385 arg1Of obtained,with
R2630 T3384 T3388 arg1Of obtained,","
R2631 T3384 T3389 modOf obtained,indicating
R2632 T3387 T3385 arg2Of splenocytes,with
R2633 T3387 T3386 arg1Of splenocytes,mouse
R2634 T3392 T3391 arg1Of interaction,the
R2635 T3392 T3393 arg1Of interaction,of
R2636 T3392 T3395 arg1Of interaction,with
R2637 T3392 T3397 arg1Of interaction,is
R2638 T3392 T3399 arg2Of interaction,limited
R2639 T3394 T3393 arg2Of CD40,of
R2640 T3396 T3395 arg2Of HOIP,with
R2641 T3399 T3389 arg2Of limited,indicating
R2642 T3399 T3390 arg1Of limited,that
R2643 T3399 T3397 arg2Of limited,is
R2644 T3399 T3398 arg1Of limited,not
R2645 T3399 T3400 arg1Of limited,to
R2646 T3404 T3400 arg2Of lines,to
R2647 T3404 T3401 arg2Of lines,transformed
R2648 T3404 T3402 arg1Of lines,B
R2649 T3404 T3403 arg1Of lines,cell
R2650 T3404 T3405 arg1Of lines,(
R2651 T3407 T3405 arg2Of observations,(
R2652 T3407 T3406 arg1Of observations,unpublished
R2653 T3408 T3405 arg3Of ),(
R2654 T3409 T3411 arg1Of We,found
R2655 T3411 T3410 arg1Of found,also
R2656 T3414 T3413 arg1Of recruitment,the
R2657 T3414 T3415 arg1Of recruitment,of
R2658 T3414 T3417 arg1Of recruitment,to
R2659 T3414 T3419 arg1Of recruitment,was
R2660 T3414 T3420 arg1Of recruitment,TRAF2-dependent
R2661 T3416 T3415 arg2Of HOIP,of
R2662 T3418 T3417 arg2Of CD40,to
R2663 T3419 T3412 arg1Of was,that
R2664 T3419 T3421 arg1Of was,and
R2665 T3420 T3419 arg2Of TRAF2-dependent,was
R2666 T3421 T3411 arg2Of and,found
R2667 T3422 T3421 arg2Of that,and
R2668 T3423 T3424 arg1Of overexpression,of
R2669 T3423 T3430 arg1Of overexpression,inhibited
R2670 T3428 T3424 arg2Of mutant,of
R2671 T3428 T3425 arg1Of mutant,a
R2672 T3428 T3426 arg2Of mutant,truncated
R2673 T3428 T3427 arg1Of mutant,HOIP
R2674 T3430 T3422 arg2Of inhibited,that
R2675 T3430 T3429 arg1Of inhibited,partially
R2676 T3433 T3430 arg2Of activation,inhibited
R2677 T3433 T3431 arg1Of activation,CD40-mediated
R2678 T3433 T3432 arg1Of activation,NF-κB
R2679 T3435 T3434 arg1Of results,These
R2680 T3435 T3436 arg1Of results,support
R2681 T3438 T3436 arg2Of hypothesis,support
R2682 T3438 T3437 arg1Of hypothesis,the
R2683 T3440 T3441 arg1Of HOIP,plays
R2684 T3441 T3438 arg2Of plays,hypothesis
R2685 T3441 T3439 arg1Of plays,that
R2686 T3441 T3444 arg1Of plays,in
R2687 T3443 T3441 arg2Of role,plays
R2688 T3443 T3442 arg1Of role,a
R2689 T3447 T3444 arg2Of transduction,in
R2690 T3447 T3445 arg1Of transduction,CD40
R2691 T3447 T3446 arg1Of transduction,signal
R2692 T3450 T3453 arg1Of define,and
R2693 T3452 T3450 arg2Of role,define
R2694 T3452 T3451 arg1Of role,the
R2695 T3453 T3448 arg1Of and,To
R2696 T3453 T3449 arg1Of and,further
R2697 T3454 T3453 arg2Of evaluate,and
R2698 T3456 T3454 arg2Of importance,evaluate
R2699 T3456 T3455 arg1Of importance,the
R2700 T3456 T3457 arg1Of importance,of
R2701 T3456 T3459 arg1Of importance,in
R2702 T3458 T3457 arg2Of HOIP,of
R2703 T3461 T3459 arg2Of signaling,in
R2704 T3461 T3460 arg1Of signaling,CD40
R2705 T3463 T3450 arg1Of we,define
R2706 T3463 T3454 arg1Of we,evaluate
R2707 T3463 T3464 arg1Of we,used
R2708 T3464 T3448 modOf used,To
R2709 T3464 T3462 arg1Of used,","
R2710 T3467 T3464 arg2Of gene,used
R2711 T3467 T3465 arg1Of gene,somatic
R2712 T3467 T3466 arg1Of gene,cell
R2713 T3467 T3468 arg1Of gene,targeting
R2714 T3468 T3469 modOf targeting,to
R2715 T3470 T3469 arg1Of disrupt,to
R2716 T3472 T3470 arg2Of gene,disrupt
R2717 T3472 T3471 arg1Of gene,the
R2718 T3472 T3473 arg1Of gene,encoding
R2719 T3473 T3475 arg1Of encoding,in
R2720 T3474 T3473 arg2Of HOIP,encoding
R2721 T3480 T3475 arg2Of line,in
R2722 T3480 T3476 arg1Of line,the
R2723 T3480 T3477 arg1Of line,mouse
R2724 T3480 T3478 arg1Of line,B
R2725 T3480 T3479 arg1Of line,cell
R2726 T3481 T3468 arg2Of A20.2J,targeting
R2727 T3484 T3482 arg1Of line,This
R2728 T3484 T3483 arg1Of line,cell
R2729 T3484 T3485 arg1Of line,has
R2730 T3484 T3486 arg1Of line,been
R2731 T3484 T3488 arg1Of line,useful
R2732 T3486 T3485 arg2Of been,has
R2733 T3486 T3514 arg1Of been,","
R2734 T3486 T3515 arg1Of been,[
R2735 T3486 T3518 arg1Of been,","
R2736 T3486 T3519 arg1Of been,[
R2737 T3488 T3486 arg2Of useful,been
R2738 T3488 T3487 arg1Of useful,particularly
R2739 T3488 T3489 arg1Of useful,in
R2740 T3488 T3497 arg1Of useful,to
R2741 T3491 T3489 arg2Of characterization,in
R2742 T3491 T3490 arg1Of characterization,the
R2743 T3491 T3492 arg1Of characterization,of
R2744 T3495 T3492 arg2Of mechanisms,of
R2745 T3495 T3493 arg1Of mechanisms,CD40
R2746 T3495 T3494 arg1Of mechanisms,signaling
R2747 T3497 T3496 arg1Of to,due
R2748 T3500 T3497 arg2Of ease,to
R2749 T3500 T3498 arg1Of ease,the
R2750 T3500 T3499 arg1Of ease,relative
R2751 T3500 T3501 arg2Of ease,with
R2752 T3500 T3502 arg1Of ease,which
R2753 T3504 T3503 arg1Of genome,its
R2754 T3504 T3505 arg1Of genome,can
R2755 T3504 T3506 arg1Of genome,be
R2756 T3504 T3507 arg2Of genome,modified
R2757 T3507 T3501 arg1Of modified,with
R2758 T3507 T3505 arg2Of modified,can
R2759 T3507 T3506 arg2Of modified,be
R2760 T3510 T3507 arg1Of recombination,modified
R2761 T3510 T3508 arg2Of recombination,by
R2762 T3510 T3509 arg1Of recombination,homologous
R2763 T3510 T3511 arg1Of recombination,[
R2764 T3512 T3511 arg2Of 7,[
R2765 T3513 T3511 arg3Of ],[
R2766 T3516 T3515 arg2Of 8,[
R2767 T3517 T3515 arg3Of ],[
R2768 T3520 T3519 arg2Of 9,[
R2769 T3521 T3519 arg3Of ],[
R2770 T3522 T3523 arg1Of We,used
R2771 T3522 T3541 arg1Of We,disrupt
R2772 T3524 T3525 arg1Of a,targeting
R2773 T3525 T3523 arg2Of targeting,used
R2774 T3525 T3524 arg1Of targeting,a
R2775 T3526 T3525 arg2Of vector,targeting
R2776 T3526 T3527 arg1Of vector,capable
R2777 T3527 T3528 arg1Of capable,of
R2778 T3529 T3528 arg2Of undergoing,of
R2779 T3529 T3532 arg1Of undergoing,with
R2780 T3531 T3529 arg2Of recombination,undergoing
R2781 T3531 T3530 arg1Of recombination,homologous
R2782 T3533 T3532 arg2Of Rnf31,with
R2783 T3533 T3534 arg1Of Rnf31,(
R2784 T3536 T3534 arg2Of gene,(
R2785 T3536 T3535 arg1Of gene,the
R2786 T3536 T3537 arg1Of gene,encoding
R2787 T3538 T3537 arg2Of HOIP,encoding
R2788 T3539 T3534 arg3Of ),(
R2789 T3541 T3523 arg3Of disrupt,used
R2790 T3541 T3540 arg1Of disrupt,to
R2791 T3544 T3541 arg2Of sequence,disrupt
R2792 T3544 T3542 arg1Of sequence,the
R2793 T3544 T3543 arg1Of sequence,coding
R2794 T3544 T3545 arg1Of sequence,of
R2795 T3544 T3548 arg1Of sequence,in
R2796 T3547 T3545 arg2Of gene,of
R2797 T3547 T3546 arg1Of gene,the
R2798 T3549 T3548 arg2Of exon,in
R2799 T3549 T3550 arg1Of exon,5
R2800 T3549 T3551 arg1Of exon,(
R2801 T3553 T3551 arg2Of 1A,(
R2802 T3553 T3552 arg1Of 1A,Fig.
R2803 T3554 T3551 arg3Of ),(
R2804 T3556 T3555 arg2Of introduction,Following
R2805 T3556 T3557 arg1Of introduction,of
R2806 T3559 T3557 arg2Of vector,of
R2807 T3559 T3558 arg1Of vector,the
R2808 T3563 T3561 arg1Of clones,the
R2809 T3563 T3562 arg1Of clones,neomycin-resistant
R2810 T3563 T3564 arg1Of clones,that
R2811 T3563 T3565 arg1Of clones,arose
R2812 T3563 T3566 arg1Of clones,were
R2813 T3563 T3567 arg2Of clones,screened
R2814 T3567 T3555 arg1Of screened,Following
R2815 T3567 T3560 arg1Of screened,","
R2816 T3567 T3566 arg2Of screened,were
R2817 T3570 T3567 arg1Of amplification,screened
R2818 T3570 T3568 arg2Of amplification,by
R2819 T3570 T3569 arg1Of amplification,PCR
R2820 T3570 T3571 arg1Of amplification,of
R2821 T3570 T3574 modOf amplification,to
R2822 T3573 T3571 arg2Of DNA,of
R2823 T3573 T3572 arg1Of DNA,genomic
R2824 T3575 T3574 arg1Of identify,to
R2825 T3576 T3575 arg2Of cells,identify
R2826 T3576 T3577 arg1Of cells,containing
R2827 T3581 T3577 arg2Of allele,containing
R2828 T3581 T3578 arg1Of allele,a
R2829 T3581 T3579 arg2Of allele,disrupted
R2830 T3581 T3580 arg1Of allele,Rnf31
R2831 T3583 T3582 arg1Of remove,To
R2832 T3588 T3583 arg2Of cassette,remove
R2833 T3588 T3584 arg1Of cassette,the
R2834 T3588 T3585 arg1Of cassette,selectable
R2835 T3588 T3586 arg1Of cassette,marker
R2836 T3588 T3587 arg1Of cassette,gene
R2837 T3588 T3589 arg1Of cassette,from
R2838 T3593 T3589 arg2Of allele,from
R2839 T3593 T3590 arg1Of allele,the
R2840 T3593 T3591 arg2Of allele,disrupted
R2841 T3593 T3592 arg1Of allele,Rnf31
R2842 T3597 T3595 arg1Of lines,recombinant
R2843 T3597 T3596 arg1Of lines,cell
R2844 T3597 T3598 arg1Of lines,were
R2845 T3597 T3600 arg2Of lines,transfected
R2846 T3600 T3582 modOf transfected,To
R2847 T3600 T3594 arg1Of transfected,","
R2848 T3600 T3598 arg2Of transfected,were
R2849 T3600 T3599 arg1Of transfected,transiently
R2850 T3600 T3601 arg1Of transfected,with
R2851 T3603 T3601 arg2Of plasmid,with
R2852 T3603 T3602 arg1Of plasmid,a
R2853 T3603 T3604 arg1Of plasmid,that
R2854 T3603 T3605 arg1Of plasmid,encodes
R2855 T3607 T3605 arg2Of recombinase,encodes
R2856 T3607 T3606 arg1Of recombinase,Cre
R2857 T3609 T3608 arg1Of step,This
R2858 T3609 T3610 arg1Of step,allowed
R2859 T3610 T3622 arg1Of allowed,","
R2860 T3610 T3623 modOf allowed,generating
R2861 T3611 T3610 arg2Of us,allowed
R2862 T3611 T3613 arg1Of us,perform
R2863 T3613 T3610 arg3Of perform,allowed
R2864 T3613 T3612 arg1Of perform,to
R2865 T3616 T3613 arg2Of round,perform
R2866 T3616 T3614 arg1Of round,a
R2867 T3616 T3615 arg1Of round,second
R2868 T3616 T3617 arg1Of round,of
R2869 T3618 T3619 arg1Of targeting,and
R2870 T3619 T3617 arg2Of and,of
R2871 T3621 T3619 arg2Of selection,and
R2872 T3621 T3620 arg1Of selection,drug
R2873 T3624 T3623 arg2Of cells,generating
R2874 T3624 T3625 arg2Of cells,in
R2875 T3624 T3626 arg1Of cells,which
R2876 T3628 T3627 arg1Of copies,both
R2877 T3628 T3629 arg1Of copies,of
R2878 T3628 T3631 arg1Of copies,were
R2879 T3628 T3632 arg2Of copies,disrupted
R2880 T3630 T3629 arg2Of Rnf31,of
R2881 T3632 T3625 arg1Of disrupted,in
R2882 T3632 T3631 arg2Of disrupted,were
R2883 T3637 T3633 arg1Of lines,Two
R2884 T3637 T3634 arg1Of lines,independent
R2885 T3637 T3635 arg1Of lines,clonal
R2886 T3637 T3636 arg1Of lines,cell
R2887 T3637 T3638 arg1Of lines,were
R2888 T3637 T3639 arg2Of lines,chosen
R2889 T3639 T3638 arg2Of chosen,were
R2890 T3639 T3640 arg1Of chosen,for
R2891 T3642 T3640 arg2Of analysis,for
R2892 T3642 T3641 arg1Of analysis,further
R2893 T3645 T3643 arg1Of expression,HOIP
R2894 T3645 T3644 arg1Of expression,protein
R2895 T3645 T3646 arg1Of expression,was
R2896 T3645 T3647 arg1Of expression,undetectable
R2897 T3646 T3652 arg1Of was,","
R2898 T3646 T3653 arg1Of was,as
R2899 T3646 T3666 arg1Of was,","
R2900 T3646 T3667 modOf was,demonstrating
R2901 T3647 T3646 arg2Of undetectable,was
R2902 T3647 T3648 arg1Of undetectable,in
R2903 T3651 T3648 arg2Of lines,in
R2904 T3651 T3649 arg1Of lines,both
R2905 T3651 T3650 arg1Of lines,cell
R2906 T3654 T3653 arg2Of determined,as
R2907 T3658 T3654 arg1Of analysis,determined
R2908 T3658 T3655 arg2Of analysis,by
R2909 T3658 T3656 arg1Of analysis,Western
R2910 T3658 T3657 arg1Of analysis,blot
R2911 T3658 T3659 arg1Of analysis,of
R2912 T3661 T3659 arg2Of lysates,of
R2913 T3661 T3660 arg1Of lysates,cell
R2914 T3661 T3662 arg1Of lysates,(
R2915 T3664 T3662 arg2Of 1B,(
R2916 T3664 T3663 arg1Of 1B,Fig.
R2917 T3665 T3662 arg3Of ),(
R2918 T3669 T3670 arg1Of the,targeting
R2919 T3670 T3669 arg1Of targeting,the
R2920 T3670 T3672 arg1Of targeting,was
R2921 T3670 T3673 arg1Of targeting,successful
R2922 T3671 T3670 arg2Of process,targeting
R2923 T3672 T3667 arg2Of was,demonstrating
R2924 T3672 T3668 arg1Of was,that
R2925 T3673 T3672 arg2Of successful,was
R2926 T3676 T3674 arg2Of text,In
R2927 T3676 T3675 arg1Of text,the
R2928 T3676 T3677 arg1Of text,that
R2929 T3676 T3678 arg1Of text,follows
R2930 T3684 T3680 arg1Of lines,the
R2931 T3684 T3681 arg1Of lines,two
R2932 T3684 T3682 arg1Of lines,gene-targeted
R2933 T3684 T3683 arg1Of lines,cell
R2934 T3684 T3685 arg1Of lines,are
R2935 T3684 T3686 arg2Of lines,referred
R2936 T3686 T3674 arg1Of referred,In
R2937 T3686 T3679 arg1Of referred,","
R2938 T3686 T3685 arg2Of referred,are
R2939 T3686 T3687 arg1Of referred,to
R2940 T3686 T3688 arg1Of referred,as
R2941 T3690 T3688 arg2Of cells,as
R2942 T3690 T3689 arg1Of cells,HOIP-deficient
R2943 T3692 T3691 arg1Of enable,To
R2944 T3693 T3692 arg2Of us,enable
R2945 T3693 T3695 arg1Of us,confirm
R2946 T3695 T3692 arg3Of confirm,enable
R2947 T3695 T3694 arg1Of confirm,to
R2948 T3698 T3699 arg1Of signaling,or
R2949 T3700 T3699 arg2Of functional,or
R2950 T3701 T3697 arg1Of defects,any
R2951 T3701 T3698 arg1Of defects,signaling
R2952 T3701 T3700 arg1Of defects,functional
R2953 T3701 T3702 arg2Of defects,observed
R2954 T3701 T3706 arg1Of defects,were
R2955 T3701 T3707 arg1Of defects,due
R2956 T3702 T3703 arg1Of observed,in
R2957 T3705 T3703 arg2Of cells,in
R2958 T3705 T3704 arg1Of cells,HOIP-deficient
R2959 T3706 T3695 arg2Of were,confirm
R2960 T3706 T3696 arg1Of were,that
R2961 T3707 T3706 arg2Of due,were
R2962 T3707 T3708 arg1Of due,to
R2963 T3709 T3708 arg2Of disruption,to
R2964 T3709 T3710 arg1Of disruption,of
R2965 T3713 T3710 arg2Of gene,of
R2966 T3713 T3711 arg1Of gene,the
R2967 T3713 T3712 arg1Of gene,Rnf31
R2968 T3718 T3715 arg1Of lines,both
R2969 T3718 T3716 arg1Of lines,HOIP-deficient
R2970 T3718 T3717 arg1Of lines,cell
R2971 T3718 T3719 arg1Of lines,were
R2972 T3718 T3720 arg2Of lines,transduced
R2973 T3718 T3730 arg1Of lines,restoring
R2974 T3720 T3691 modOf transduced,To
R2975 T3720 T3714 arg1Of transduced,","
R2976 T3720 T3719 arg2Of transduced,were
R2977 T3720 T3721 arg1Of transduced,with
R2978 T3720 T3728 arg1Of transduced,","
R2979 T3720 T3729 arg1Of transduced,thus
R2980 T3720 T3730 modOf transduced,restoring
R2981 T3723 T3721 arg2Of retrovirus,with
R2982 T3723 T3722 arg1Of retrovirus,a
R2983 T3723 T3724 arg1Of retrovirus,encoding
R2984 T3727 T3724 arg2Of HOIP,encoding
R2985 T3727 T3725 arg1Of HOIP,FLAG-tagged
R2986 T3727 T3726 arg1Of HOIP,wild-type
R2987 T3733 T3730 arg2Of expression,restoring
R2988 T3733 T3731 arg1Of expression,HOIP
R2989 T3733 T3732 arg1Of expression,protein
R2990 T3733 T3734 arg1Of expression,(
R2991 T3736 T3734 arg2Of 1B,(
R2992 T3736 T3735 arg1Of 1B,Fig.
R2993 T3737 T3734 arg3Of ),(
R2994 T3739 T3738 arg1Of cells,These
R2995 T3739 T3740 arg1Of cells,are
R2996 T3739 T3741 arg2Of cells,referred
R2997 T3741 T3740 arg2Of referred,are
R2998 T3741 T3742 arg1Of referred,to
R2999 T3741 T3743 arg1Of referred,as
R3000 T3745 T3743 arg2Of cells,as
R3001 T3745 T3744 arg1Of cells,HOIP-reconstituted
R3002 T3748 T3746 arg2Of experiments,In
R3003 T3748 T3747 arg1Of experiments,the
R3004 T3748 T3749 arg1Of experiments,that
R3005 T3748 T3750 arg1Of experiments,follow
R3006 T3752 T3753 arg1Of responses,by
R3007 T3752 T3756 arg1Of responses,were
R3008 T3752 T3757 arg2Of responses,compared
R3009 T3755 T3753 arg2Of cells,by
R3010 T3755 T3754 arg1Of cells,these
R3011 T3757 T3746 arg1Of compared,In
R3012 T3757 T3751 arg1Of compared,","
R3013 T3757 T3756 arg2Of compared,were
R3014 T3757 T3758 arg1Of compared,to
R3015 T3759 T3758 arg2Of those,to
R3016 T3759 T3760 arg1Of those,of
R3017 T3762 T3761 arg1Of cells,A20.2J
R3018 T3762 T3763 arg1Of cells,and
R3019 T3763 T3760 arg2Of and,of
R3020 T3763 T3766 arg2Of and,transduced
R3021 T3765 T3763 arg2Of cells,and
R3022 T3765 T3764 arg1Of cells,HOIP-deficient
R3023 T3766 T3767 arg1Of transduced,with
R3024 T3770 T3767 arg2Of vector,with
R3025 T3770 T3768 arg1Of vector,a
R3026 T3770 T3769 arg1Of vector,retroviral
R3027 T3770 T3771 arg1Of vector,(
R3028 T3770 T3774 arg1Of vector,lacking
R3029 T3772 T3771 arg2Of pMIP,(
R3030 T3773 T3771 arg3Of ),(
R3031 T3777 T3774 arg2Of insert,lacking
R3032 T3777 T3775 arg1Of insert,a
R3033 T3777 T3776 arg1Of insert,cDNA
R3034 T3777 T3778 arg1Of insert,(
R3035 T3780 T3778 arg2Of vector,(
R3036 T3780 T3779 arg1Of vector,empty
R3037 T3781 T3778 arg3Of ),(
R11923 T15029 T15027 arg1Of targeting,HOIP
R11924 T15029 T15028 arg1Of targeting,gene
R11925 T15031 T15030 arg2Of A,(
R11926 T15032 T15030 arg3Of ),(
R11927 T15033 T15030 arg1Of Regions,(
R11928 T15033 T15034 arg1Of Regions,of
R11929 T15033 T15041 arg2Of Regions,used
R11930 T15033 T15051 arg1Of Regions,are
R11931 T15033 T15052 arg2Of Regions,shown
R11934 T15037 T15036 arg1Of sequence,gene
R11935 T15037 T15038 arg1Of sequence,(
R11936 T15039 T15038 arg2Of bottom,(
R11937 T15040 T15038 arg3Of ),(
R11938 T15041 T15042 arg1Of used,in
R11939 T15047 T15042 arg2Of vector,in
R11940 T15047 T15043 arg1Of vector,the
R11941 T15047 T15044 arg1Of vector,HOIP
R11942 T15047 T15045 arg1Of vector,gene
R11943 T15047 T15046 arg1Of vector,targeting
R11944 T15047 T15048 arg1Of vector,(
R11945 T15049 T15048 arg2Of top,(
R11946 T15050 T15048 arg3Of ),(
R11947 T15052 T15051 arg2Of shown,are
R11948 T15053 T15054 arg1Of Arrows,(
R11949 T15053 T15059 arg1Of Arrows,indicate
R11950 T15055 T15056 arg1Of F,and
R11951 T15056 T15054 arg2Of and,(
R11952 T15057 T15056 arg2Of R,and
R11953 T15058 T15054 arg3Of ),(
R11954 T15061 T15059 arg2Of positions,indicate
R11955 T15061 T15060 arg1Of positions,approximate
R11956 T15061 T15062 arg1Of positions,of
R11957 T15063 T15062 arg2Of sequences,of
R11958 T15063 T15064 arg1Of sequences,homologous
R11959 T15064 T15065 arg1Of homologous,to
R11960 T15066 T15065 arg2Of oligonucleotides,to
R11961 T15066 T15067 arg2Of oligonucleotides,used
R11962 T15067 T15068 arg1Of used,for
R11963 T15070 T15068 arg2Of detection,for
R11964 T15070 T15069 arg1Of detection,PCR-mediated
R11965 T15070 T15071 arg1Of detection,of
R11966 T15073 T15071 arg2Of recombination,of
R11967 T15073 T15072 arg1Of recombination,homologous
R11968 T15075 T15074 arg1Of recombination,Homologous
R11969 T15075 T15076 arg1Of recombination,of
R11970 T15075 T15081 arg1Of recombination,resulted
R11971 T15078 T15076 arg2Of vector,of
R11972 T15078 T15077 arg1Of vector,the
R11973 T15078 T15079 arg1Of vector,with
R11974 T15080 T15079 arg2Of Rnf31,with
R11975 T15081 T15082 arg1Of resulted,in
R11976 T15086 T15083 arg1Of deletion,a
R11977 T15086 T15084 arg1Of deletion,small
R11978 T15086 T15085 arg1Of deletion,chromosomal
R11979 T15086 T15087 arg1Of deletion,and
R11980 T15087 T15082 arg2Of and,in
R11981 T15090 T15087 arg2Of insertion,and
R11982 T15090 T15088 arg1Of insertion,the
R11983 T15090 T15089 arg1Of insertion,in-frame
R11984 T15090 T15091 arg1Of insertion,of
R11985 T15090 T15097 arg1Of insertion,into
R11986 T15093 T15091 arg2Of phosphotransferase,of
R11987 T15093 T15092 arg1Of phosphotransferase,neomycin
R11988 T15093 T15094 arg1Of phosphotransferase,(
R11989 T15095 T15094 arg2Of NeoR,(
R11990 T15096 T15094 arg3Of ),(
R11991 T15100 T15097 arg2Of HOIP,into
R11992 T15100 T15098 arg1Of HOIP,the
R11993 T15100 T15099 arg1Of HOIP,amino-terminal
R11994 T15100 T15101 arg1Of HOIP,coding
R11995 T15102 T15101 arg2Of sequence,coding
R11996 T15105 T15104 arg1Of toxin,diphtheria
R11997 T15105 T15106 arg1Of toxin,(
R11998 T15107 T15106 arg2Of DT,(
R11999 T15108 T15106 arg3Of ),(
R12000 T15109 T15103 arg1Of cassette,A
R12001 T15109 T15105 arg1Of cassette,toxin
R12002 T15109 T15110 arg1Of cassette,in
R12003 T15109 T15113 arg1Of cassette,facilitates
R12004 T15112 T15110 arg2Of vector,in
R12005 T15112 T15111 arg1Of vector,the
R12006 T15116 T15113 arg2Of selection,facilitates
R12007 T15116 T15114 arg1Of selection,the
R12008 T15116 T15115 arg1Of selection,negative
R12009 T15116 T15117 arg1Of selection,of
R12010 T15118 T15117 arg2Of cells,of
R12011 T15118 T15119 arg1Of cells,in
R12012 T15118 T15120 arg1Of cells,which
R12013 T15118 T15121 arg1Of cells,random
R12014 T15123 T15121 arg2Of insertion,random
R12015 T15123 T15122 arg1Of insertion,chromosomal
R12016 T15123 T15124 arg1Of insertion,of
R12017 T15123 T15127 arg1Of insertion,takes
R12018 T15126 T15124 arg2Of vector,of
R12019 T15126 T15125 arg1Of vector,the
R12020 T15127 T15121 arg3Of takes,random
R12021 T15128 T15127 arg2Of place,takes
R12022 T15130 T15129 arg1Of sequences,LoxP
R12023 T15130 T15131 arg1Of sequences,allow
R12024 T15134 T15131 arg2Of deletion,allow
R12025 T15134 T15132 arg1Of deletion,the
R12026 T15134 T15133 arg1Of deletion,Cre-mediated
R12027 T15134 T15135 arg1Of deletion,of
R12028 T15139 T15135 arg2Of sequence,of
R12029 T15139 T15136 arg1Of sequence,the
R12030 T15139 T15137 arg1Of sequence,NeoR
R12031 T15139 T15138 arg1Of sequence,coding
R12032 T15142 T15140 arg1Of sequence,The
R12033 T15142 T15141 arg1Of sequence,SV40pA
R12034 T15142 T15143 arg1Of sequence,helps
R12035 T15142 T15145 arg1Of sequence,ensure
R12036 T15145 T15143 arg2Of ensure,helps
R12037 T15145 T15144 arg1Of ensure,to
R12038 T15146 T15147 arg1Of disruption,of
R12039 T15146 T15150 arg1Of disruption,after
R12040 T15146 T15160 arg1Of disruption,and
R12041 T15149 T15147 arg2Of expression,of
R12042 T15149 T15148 arg1Of expression,gene
R12043 T15151 T15150 arg2Of deletion,after
R12044 T15151 T15152 arg1Of deletion,of
R12045 T15155 T15154 arg1Of sequence.,NeoR
R12046 T15155 T15156 arg1Of sequence.,(
R12047 T15157 T15156 arg2Of B,(
R12048 T15158 T15156 arg3Of ),(
R12049 T15159 T15152 arg2Of Anti-HOIP,of
R12050 T15159 T15153 arg1Of Anti-HOIP,the
R12051 T15159 T15155 arg1Of Anti-HOIP,sequence.
R12052 T15160 T15145 arg2Of and,ensure
R12053 T15163 T15160 arg2Of blots,and
R12054 T15163 T15161 arg1Of blots,anti-FLAG
R12055 T15163 T15162 arg1Of blots,Western
R12056 T15163 T15164 arg1Of blots,of
R12057 T15166 T15165 arg1Of lysates,cell
R12058 T15166 T15167 arg1Of lysates,from
R12059 T15166 T15170 arg1Of lysates,and
R12060 T15169 T15167 arg2Of cells,from
R12061 T15169 T15168 arg1Of cells,A20.2J
R12062 T15170 T15164 arg2Of and,of
R12063 T15171 T15172 arg1Of HOIP-deficient,(
R12064 T15173 T15172 arg2Of HOIP-/-,(
R12065 T15174 T15172 arg3Of ),(
R12066 T15175 T15170 arg2Of clones,and
R12067 T15175 T15171 arg1Of clones,HOIP-deficient
R12068 T15178 T15176 arg1Of decrease,A
R12069 T15178 T15177 arg1Of decrease,partial
R12070 T15178 T15179 arg1Of decrease,in
R12071 T15178 T15183 arg1Of decrease,is
R12072 T15178 T15184 arg1Of decrease,evident
R12073 T15182 T15179 arg2Of expression,in
R12074 T15182 T15180 arg1Of expression,HOIP
R12075 T15182 T15181 arg1Of expression,protein
R12076 T15183 T15185 arg1Of is,in
R12077 T15183 T15187 arg1Of is,following
R12078 T15184 T15183 arg2Of evident,is
R12079 T15186 T15185 arg2Of cells,in
R12080 T15188 T15187 arg2Of disruption,following
R12081 T15188 T15189 arg1Of disruption,of
R12082 T15191 T15189 arg2Of copy,of
R12083 T15191 T15190 arg1Of copy,one
R12084 T15191 T15192 arg1Of copy,of
R12085 T15193 T15192 arg2Of Rnf31,of
R12086 T15193 T15194 arg1Of Rnf31,(
R12087 T15195 T15194 arg2Of HOIP−/+,(
R12088 T15196 T15194 arg3Of ),(
R12089 T15198 T15197 arg1Of expression,HOIP
R12090 T15198 T15199 arg1Of expression,in
R12091 T15198 T15217 arg1Of expression,is
R12092 T15198 T15219 arg2Of expression,shown
R12093 T15200 T15199 arg2Of clones,in
R12094 T15200 T15201 arg2Of clones,reconstituted
R12095 T15201 T15202 arg1Of reconstituted,with
R12096 T15206 T15203 arg1Of vector,an
R12097 T15206 T15204 arg1Of vector,empty
R12098 T15206 T15205 arg1Of vector,retroviral
R12099 T15206 T15207 arg1Of vector,(
R12100 T15206 T15210 arg1Of vector,or
R12101 T15208 T15207 arg2Of pMIP,(
R12102 T15209 T15207 arg3Of ),(
R12103 T15210 T15202 arg2Of or,with
R12104 T15213 T15210 arg2Of vector,or
R12105 T15213 T15211 arg1Of vector,a
R12106 T15213 T15212 arg1Of vector,retroviral
R12107 T15213 T15214 arg1Of vector,encoding
R12108 T15216 T15214 arg2Of HOIP,encoding
R12109 T15216 T15215 arg1Of HOIP,FLAG-tagged
R12110 T15219 T15217 arg2Of shown,is
R12111 T15219 T15218 arg1Of shown,also
R12112 T15222 T15220 arg1Of weight,Approximate
R12113 T15222 T15221 arg1Of weight,molecular
R12114 T15222 T15223 arg1Of weight,of
R12115 T15222 T15225 arg1Of weight,is
R12116 T15224 T15223 arg2Of HOIP,of
R12117 T15227 T15225 arg2Of kD,is
R12118 T15227 T15226 arg1Of kD,120
R11932 T15037 T15034 arg2Of sequence,of
R11933 T15037 T15035 arg1Of sequence,Rnf31

2_test

Id Subject Object Predicate Lexical cue
21829693-20614026-89653758 213-214 20614026 denotes 6
21829693-12958312-89653759 1029-1030 12958312 denotes 7
21829693-17878362-89653760 1034-1035 17878362 denotes 8
21829693-14981114-89653761 1039-1040 14981114 denotes 9
T45718 213-214 20614026 denotes 6
T92919 1029-1030 12958312 denotes 7
T70074 1034-1035 17878362 denotes 8
T10373 1039-1040 14981114 denotes 9

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T2880 0-10 NNP denotes Generation
T2881 11-13 IN denotes of
T2882 14-28 NNP denotes HOIP-deficient
T2883 29-30 NNP denotes B
T2884 31-36 NNS denotes cells
T2885 37-40 IN denotes via
T2886 41-49 JJ denotes targeted
T2887 50-60 NN denotes disruption
T2888 61-63 IN denotes of
T2889 64-69 NNP denotes Rnf31
T2890 69-70 , denotes ,
T2891 71-74 DT denotes the
T2892 75-79 NN denotes gene
T2893 80-88 VBG denotes encoding
T2894 89-93 NNP denotes HOIP
T2895 94-96 IN denotes In
T2896 97-100 PRP$ denotes our
T2897 101-109 JJ denotes previous
T2898 110-115 NN denotes study
T2899 115-116 , denotes ,
T2900 117-119 PRP denotes we
T2901 120-132 VBD denotes demonstrated
T2902 133-137 IN denotes that
T2903 138-142 NNP denotes HOIP
T2904 143-145 VBZ denotes is
T2905 146-155 VBN denotes recruited
T2906 156-158 TO denotes to
T2907 159-162 DT denotes the
T2908 163-167 NNP denotes CD40
T2909 168-177 VBG denotes signaling
T2910 178-185 JJ denotes complex
T2911 186-188 IN denotes in
T2912 189-192 CD denotes two
T2913 193-198 NN denotes mouse
T2914 199-200 NNP denotes B
T2915 201-205 NN denotes cell
T2916 206-211 NNS denotes lines
T2917 212-213 VBP denotes [
T2918 213-214 CD denotes 6
T2919 214-215 NNP denotes ]
T2920 215-216 . denotes .
T2921 217-224 JJ denotes Similar
T2922 225-232 NNS denotes results
T2923 233-237 VBD denotes were
T2924 238-246 VBN denotes obtained
T2925 247-251 IN denotes with
T2926 252-257 NN denotes mouse
T2927 258-269 NNS denotes splenocytes
T2928 269-270 , denotes ,
T2929 271-281 VBG denotes indicating
T2930 282-286 IN denotes that
T2931 287-290 DT denotes the
T2932 291-302 NN denotes interaction
T2933 303-305 IN denotes of
T2934 306-310 CD denotes CD40
T2935 311-315 IN denotes with
T2936 316-320 NNP denotes HOIP
T2937 321-323 VBZ denotes is
T2938 324-327 RB denotes not
T2939 328-335 VBN denotes limited
T2940 336-338 TO denotes to
T2941 339-350 VBN denotes transformed
T2942 351-352 NNP denotes B
T2943 353-357 NN denotes cell
T2944 358-363 NNS denotes lines
T2945 364-365 -LRB- denotes (
T2946 365-376 JJ denotes unpublished
T2947 377-389 NNS denotes observations
T2948 389-390 -RRB- denotes )
T2949 390-391 . denotes .
T2950 392-394 PRP denotes We
T2951 395-399 RB denotes also
T2952 400-405 VBD denotes found
T2953 406-410 IN denotes that
T2954 411-414 DT denotes the
T2955 415-426 NN denotes recruitment
T2956 427-429 IN denotes of
T2957 430-434 NNP denotes HOIP
T2958 435-437 TO denotes to
T2959 438-442 NNP denotes CD40
T2960 443-446 VBD denotes was
T2961 447-462 JJ denotes TRAF2-dependent
T2962 463-466 CC denotes and
T2963 467-471 IN denotes that
T2964 472-486 NN denotes overexpression
T2965 487-489 IN denotes of
T2966 490-491 DT denotes a
T2967 492-501 VBN denotes truncated
T2968 502-506 NNP denotes HOIP
T2969 507-513 JJ denotes mutant
T2970 514-523 RB denotes partially
T2971 524-533 VBN denotes inhibited
T2972 534-547 JJ denotes CD40-mediated
T2973 548-553 NN denotes NF-κB
T2974 554-564 NN denotes activation
T2975 564-565 . denotes .
T2976 566-571 DT denotes These
T2977 572-579 NNS denotes results
T2978 580-587 VBP denotes support
T2979 588-591 DT denotes the
T2980 592-602 NN denotes hypothesis
T2981 603-607 WDT denotes that
T2982 608-612 NNP denotes HOIP
T2983 613-618 VBZ denotes plays
T2984 619-620 DT denotes a
T2985 621-625 NN denotes role
T2986 626-628 IN denotes in
T2987 629-633 CD denotes CD40
T2988 634-640 NN denotes signal
T2989 641-653 NN denotes transduction
T2990 653-654 . denotes .
T2991 655-657 TO denotes To
T2992 658-665 RBR denotes further
T2993 666-672 VB denotes define
T2994 673-676 DT denotes the
T2995 677-681 NN denotes role
T2996 682-685 CC denotes and
T2997 686-694 VB denotes evaluate
T2998 695-698 DT denotes the
T2999 699-709 NN denotes importance
T3000 710-712 IN denotes of
T3001 713-717 NNP denotes HOIP
T3002 718-720 IN denotes in
T3003 721-725 NNP denotes CD40
T3004 726-735 VBG denotes signaling
T3005 735-736 , denotes ,
T3006 737-739 PRP denotes we
T3007 740-744 VBD denotes used
T3008 745-752 JJ denotes somatic
T3009 753-757 NN denotes cell
T3010 758-762 NN denotes gene
T3011 763-772 VBG denotes targeting
T3012 773-775 TO denotes to
T3013 776-783 VB denotes disrupt
T3014 784-787 DT denotes the
T3015 788-792 NN denotes gene
T3016 793-801 VBG denotes encoding
T3017 802-806 NNP denotes HOIP
T3018 807-809 IN denotes in
T3019 810-813 DT denotes the
T3020 814-819 NN denotes mouse
T3021 820-821 NNP denotes B
T3022 822-826 NN denotes cell
T3023 827-831 NN denotes line
T3024 832-835 NNP denotes A20
T3025 835-837 CD denotes .2
T3026 837-838 NNP denotes J
T3027 838-839 . denotes .
T3028 840-844 DT denotes This
T3029 845-849 NN denotes cell
T3030 850-854 NN denotes line
T3031 855-858 VBZ denotes has
T3032 859-863 VBN denotes been
T3033 864-876 RB denotes particularly
T3034 877-883 JJ denotes useful
T3035 884-886 IN denotes in
T3036 887-890 DT denotes the
T3037 891-907 NN denotes characterization
T3038 908-910 IN denotes of
T3039 911-915 NNP denotes CD40
T3040 916-925 VBG denotes signaling
T3041 926-936 NNS denotes mechanisms
T3042 937-940 JJ denotes due
T3043 941-943 TO denotes to
T3044 944-947 DT denotes the
T3045 948-956 JJ denotes relative
T3046 957-961 NN denotes ease
T3047 962-966 IN denotes with
T3048 967-972 WDT denotes which
T3049 973-976 PRP$ denotes its
T3050 977-983 NN denotes genome
T3051 984-987 MD denotes can
T3052 988-990 VB denotes be
T3053 991-999 VBN denotes modified
T3054 1000-1002 IN denotes by
T3055 1003-1013 RB denotes homologous
T3056 1014-1027 NN denotes recombination
T3057 1028-1029 NN denotes [
T3058 1029-1030 CD denotes 7
T3059 1030-1031 NNP denotes ]
T3060 1031-1032 , denotes ,
T3061 1033-1034 NNP denotes [
T3062 1034-1035 CD denotes 8
T3063 1035-1036 NNP denotes ]
T3064 1036-1037 , denotes ,
T3065 1038-1039 NNP denotes [
T3066 1039-1040 CD denotes 9
T3067 1040-1041 NNP denotes ]
T3068 1041-1042 . denotes .
T3069 1043-1045 PRP denotes We
T3070 1046-1050 VBD denotes used
T3071 1051-1052 DT denotes a
T3072 1053-1062 VBG denotes targeting
T3073 1063-1069 NN denotes vector
T3074 1070-1077 JJ denotes capable
T3075 1078-1080 IN denotes of
T3076 1081-1091 VBG denotes undergoing
T3077 1092-1102 RB denotes homologous
T3078 1103-1116 NN denotes recombination
T3079 1117-1121 IN denotes with
T3080 1122-1127 NNP denotes Rnf31
T3081 1128-1129 -LRB- denotes (
T3082 1129-1132 DT denotes the
T3083 1133-1137 NN denotes gene
T3084 1138-1146 VBG denotes encoding
T3085 1147-1151 NNP denotes HOIP
T3086 1151-1152 -RRB- denotes )
T3087 1153-1155 TO denotes to
T3088 1156-1163 VB denotes disrupt
T3089 1164-1167 DT denotes the
T3090 1168-1174 NN denotes coding
T3091 1175-1183 NN denotes sequence
T3092 1184-1186 IN denotes of
T3093 1187-1190 DT denotes the
T3094 1191-1195 NN denotes gene
T3095 1196-1198 IN denotes in
T3096 1199-1203 NN denotes exon
T3097 1204-1205 CD denotes 5
T3098 1206-1207 -LRB- denotes (
T3099 1207-1211 NNP denotes Fig.
T3100 1212-1214 NNP denotes 1A
T3101 1214-1215 -RRB- denotes )
T3102 1215-1216 . denotes .
T3103 1217-1226 VBG denotes Following
T3104 1227-1239 NN denotes introduction
T3105 1240-1242 IN denotes of
T3106 1243-1246 DT denotes the
T3107 1247-1253 NN denotes vector
T3108 1253-1254 , denotes ,
T3109 1255-1258 DT denotes the
T3110 1259-1277 JJ denotes neomycin-resistant
T3111 1278-1284 NNS denotes clones
T3112 1285-1289 WDT denotes that
T3113 1290-1295 VBD denotes arose
T3114 1296-1300 VBD denotes were
T3115 1301-1309 VBN denotes screened
T3116 1310-1312 IN denotes by
T3117 1313-1316 NNP denotes PCR
T3118 1317-1330 NN denotes amplification
T3119 1331-1333 IN denotes of
T3120 1334-1341 JJ denotes genomic
T3121 1342-1345 NN denotes DNA
T3122 1346-1348 TO denotes to
T3123 1349-1357 VB denotes identify
T3124 1358-1363 NNS denotes cells
T3125 1364-1374 VBG denotes containing
T3126 1375-1376 DT denotes a
T3127 1377-1386 VBN denotes disrupted
T3128 1387-1392 NNP denotes Rnf31
T3129 1393-1399 NN denotes allele
T3130 1399-1400 . denotes .
T3131 1401-1403 TO denotes To
T3132 1404-1410 VB denotes remove
T3133 1411-1414 DT denotes the
T3134 1415-1425 JJ denotes selectable
T3135 1426-1432 NN denotes marker
T3136 1433-1437 NN denotes gene
T3137 1438-1446 NN denotes cassette
T3138 1447-1451 IN denotes from
T3139 1452-1455 DT denotes the
T3140 1456-1465 VBN denotes disrupted
T3141 1466-1471 NNP denotes Rnf31
T3142 1472-1478 NN denotes allele
T3143 1478-1479 , denotes ,
T3144 1480-1491 JJ denotes recombinant
T3145 1492-1496 NN denotes cell
T3146 1497-1502 NNS denotes lines
T3147 1503-1507 VBD denotes were
T3148 1508-1519 RB denotes transiently
T3149 1520-1531 VBN denotes transfected
T3150 1532-1536 IN denotes with
T3151 1537-1538 DT denotes a
T3152 1539-1546 NN denotes plasmid
T3153 1547-1551 WDT denotes that
T3154 1552-1559 VBZ denotes encodes
T3155 1560-1563 NNP denotes Cre
T3156 1564-1575 NN denotes recombinase
T3157 1575-1576 . denotes .
T3158 1577-1581 DT denotes This
T3159 1582-1586 NN denotes step
T3160 1587-1594 VBD denotes allowed
T3161 1595-1597 PRP denotes us
T3162 1598-1600 TO denotes to
T3163 1601-1608 VB denotes perform
T3164 1609-1610 DT denotes a
T3165 1611-1617 JJ denotes second
T3166 1618-1623 NN denotes round
T3167 1624-1626 IN denotes of
T3168 1627-1636 VBG denotes targeting
T3169 1637-1640 CC denotes and
T3170 1641-1645 NN denotes drug
T3171 1646-1655 NN denotes selection
T3172 1655-1656 , denotes ,
T3173 1657-1667 VBG denotes generating
T3174 1668-1673 NNS denotes cells
T3175 1674-1676 IN denotes in
T3176 1677-1682 WDT denotes which
T3177 1683-1687 DT denotes both
T3178 1688-1694 NNS denotes copies
T3179 1695-1697 IN denotes of
T3180 1698-1703 NNS denotes Rnf31
T3181 1704-1708 VBD denotes were
T3182 1709-1718 VBN denotes disrupted
T3183 1718-1719 . denotes .
T3184 1720-1723 CD denotes Two
T3185 1724-1735 JJ denotes independent
T3186 1736-1742 JJ denotes clonal
T3187 1743-1747 NN denotes cell
T3188 1748-1753 NNS denotes lines
T3189 1754-1758 VBD denotes were
T3190 1759-1765 VBN denotes chosen
T3191 1766-1769 IN denotes for
T3192 1770-1777 JJ denotes further
T3193 1778-1786 NN denotes analysis
T3194 1786-1787 . denotes .
T3195 1788-1792 NNP denotes HOIP
T3196 1793-1800 NN denotes protein
T3197 1801-1811 NN denotes expression
T3198 1812-1815 VBD denotes was
T3199 1816-1828 JJ denotes undetectable
T3200 1829-1831 IN denotes in
T3201 1832-1836 DT denotes both
T3202 1837-1841 NN denotes cell
T3203 1842-1847 NNS denotes lines
T3204 1847-1848 , denotes ,
T3205 1849-1851 IN denotes as
T3206 1852-1862 VBN denotes determined
T3207 1863-1865 IN denotes by
T3208 1866-1873 JJ denotes Western
T3209 1874-1878 NN denotes blot
T3210 1879-1887 NN denotes analysis
T3211 1888-1890 IN denotes of
T3212 1891-1895 NN denotes cell
T3213 1896-1903 NNS denotes lysates
T3214 1904-1905 -LRB- denotes (
T3215 1905-1909 NNP denotes Fig.
T3216 1910-1912 NNP denotes 1B
T3217 1912-1913 -RRB- denotes )
T3218 1913-1914 , denotes ,
T3219 1915-1928 VBG denotes demonstrating
T3220 1929-1933 IN denotes that
T3221 1934-1937 DT denotes the
T3222 1938-1947 VBG denotes targeting
T3223 1948-1955 NN denotes process
T3224 1956-1959 VBD denotes was
T3225 1960-1970 JJ denotes successful
T3226 1970-1971 . denotes .
T3227 1972-1974 IN denotes In
T3228 1975-1978 DT denotes the
T3229 1979-1983 NN denotes text
T3230 1984-1988 WDT denotes that
T3231 1989-1996 VBZ denotes follows
T3232 1996-1997 , denotes ,
T3233 1998-2001 DT denotes the
T3234 2002-2005 CD denotes two
T3235 2006-2019 JJ denotes gene-targeted
T3236 2020-2024 NN denotes cell
T3237 2025-2030 NNS denotes lines
T3238 2031-2034 VBP denotes are
T3239 2035-2043 VBN denotes referred
T3240 2044-2046 TO denotes to
T3241 2047-2049 IN denotes as
T3242 2050-2064 JJ denotes HOIP-deficient
T3243 2065-2070 NNS denotes cells
T3244 2070-2071 . denotes .
T3245 3352-3354 TO denotes To
T3246 3355-3361 VB denotes enable
T3247 3362-3364 PRP denotes us
T3248 3365-3367 TO denotes to
T3249 3368-3375 VB denotes confirm
T3250 3376-3380 IN denotes that
T3251 3381-3384 DT denotes any
T3252 3385-3394 VBG denotes signaling
T3253 3395-3397 CC denotes or
T3254 3398-3408 JJ denotes functional
T3255 3409-3416 NNS denotes defects
T3256 3417-3425 VBD denotes observed
T3257 3426-3428 IN denotes in
T3258 3429-3443 JJ denotes HOIP-deficient
T3259 3444-3449 NNS denotes cells
T3260 3450-3454 VBD denotes were
T3261 3455-3458 JJ denotes due
T3262 3459-3461 TO denotes to
T3263 3462-3472 NN denotes disruption
T3264 3473-3475 IN denotes of
T3265 3476-3479 DT denotes the
T3266 3480-3485 NNP denotes Rnf31
T3267 3486-3490 NN denotes gene
T3268 3490-3491 , denotes ,
T3269 3492-3496 DT denotes both
T3270 3497-3511 JJ denotes HOIP-deficient
T3271 3512-3516 NN denotes cell
T3272 3517-3522 NNS denotes lines
T3273 3523-3527 VBD denotes were
T3274 3528-3538 VBN denotes transduced
T3275 3539-3543 IN denotes with
T3276 3544-3545 DT denotes a
T3277 3546-3556 NN denotes retrovirus
T3278 3557-3565 VBG denotes encoding
T3279 3566-3577 JJ denotes FLAG-tagged
T3280 3578-3587 JJ denotes wild-type
T3281 3588-3592 NNP denotes HOIP
T3282 3592-3593 , denotes ,
T3283 3594-3598 RB denotes thus
T3284 3599-3608 VBG denotes restoring
T3285 3609-3613 NNP denotes HOIP
T3286 3614-3621 NN denotes protein
T3287 3622-3632 NN denotes expression
T3288 3633-3634 -LRB- denotes (
T3289 3634-3638 NNP denotes Fig.
T3290 3639-3641 NNP denotes 1B
T3291 3641-3642 -RRB- denotes )
T3292 3642-3643 . denotes .
T3293 3644-3649 DT denotes These
T3294 3650-3655 NNS denotes cells
T3295 3656-3659 VBP denotes are
T3296 3660-3668 VBN denotes referred
T3297 3669-3671 TO denotes to
T3298 3672-3674 IN denotes as
T3299 3675-3693 JJ denotes HOIP-reconstituted
T3300 3694-3699 NNS denotes cells
T3301 3699-3700 . denotes .
T3302 3701-3703 IN denotes In
T3303 3704-3707 DT denotes the
T3304 3708-3719 NNS denotes experiments
T3305 3720-3724 WDT denotes that
T3306 3725-3731 VBP denotes follow
T3307 3731-3732 , denotes ,
T3308 3733-3742 NNS denotes responses
T3309 3743-3745 IN denotes by
T3310 3746-3751 DT denotes these
T3311 3752-3757 NNS denotes cells
T3312 3758-3762 VBD denotes were
T3313 3763-3771 VBN denotes compared
T3314 3772-3774 TO denotes to
T3315 3775-3780 DT denotes those
T3316 3781-3783 IN denotes of
T3317 3784-3787 NNP denotes A20
T3318 3787-3789 CD denotes .2
T3319 3789-3790 NNP denotes J
T3320 3791-3796 NNS denotes cells
T3321 3797-3800 CC denotes and
T3322 3801-3815 JJ denotes HOIP-deficient
T3323 3816-3821 NNS denotes cells
T3324 3822-3832 VBN denotes transduced
T3325 3833-3837 IN denotes with
T3326 3838-3839 DT denotes a
T3327 3840-3850 JJ denotes retroviral
T3328 3851-3857 NN denotes vector
T3329 3858-3859 -LRB- denotes (
T3330 3859-3863 NNP denotes pMIP
T3331 3863-3864 -RRB- denotes )
T3332 3865-3872 VBG denotes lacking
T3333 3873-3874 DT denotes a
T3334 3875-3879 NNP denotes cDNA
T3335 3880-3886 NN denotes insert
T3336 3887-3888 -LRB- denotes (
T3337 3888-3893 JJ denotes empty
T3338 3894-3900 NN denotes vector
T3339 3900-3901 -RRB- denotes )
T3340 3901-3902 . denotes .
T14807 2116-2120 NNP denotes HOIP
T14808 2121-2125 NN denotes gene
T14809 2126-2135 VBG denotes targeting
T14810 2135-2136 . denotes .
T14811 2137-2138 -LRB- denotes (
T14812 2138-2139 NNP denotes A
T14813 2139-2140 -RRB- denotes )
T14814 2141-2148 NNS denotes Regions
T14815 2149-2151 IN denotes of
T14816 2152-2157 JJ denotes Rnf31
T14817 2158-2162 NN denotes gene
T14818 2163-2171 NN denotes sequence
T14819 2172-2173 -LRB- denotes (
T14820 2173-2179 NN denotes bottom
T14821 2179-2180 -RRB- denotes )
T14822 2181-2185 VBN denotes used
T14823 2186-2188 IN denotes in
T14824 2189-2192 DT denotes the
T14825 2193-2197 NNP denotes HOIP
T14826 2198-2202 NN denotes gene
T14827 2203-2212 VBG denotes targeting
T14828 2213-2219 NN denotes vector
T14829 2220-2221 -LRB- denotes (
T14830 2221-2224 JJ denotes top
T14831 2224-2225 -RRB- denotes )
T14832 2226-2229 VBP denotes are
T14833 2230-2235 VBN denotes shown
T14834 2235-2236 . denotes .
T14835 2237-2243 NNS denotes Arrows
T14836 2244-2245 -LRB- denotes (
T14837 2245-2246 NN denotes F
T14838 2247-2250 CC denotes and
T14839 2251-2252 NN denotes R
T14840 2252-2253 -RRB- denotes )
T14841 2254-2262 VBP denotes indicate
T14842 2263-2274 JJ denotes approximate
T14843 2275-2284 NNS denotes positions
T14844 2285-2287 IN denotes of
T14845 2288-2297 NNS denotes sequences
T14846 2298-2308 RB denotes homologous
T14847 2309-2311 TO denotes to
T14848 2312-2328 NNS denotes oligonucleotides
T14849 2329-2333 VBN denotes used
T14850 2334-2337 IN denotes for
T14851 2338-2350 JJ denotes PCR-mediated
T14852 2351-2360 NN denotes detection
T14853 2361-2363 IN denotes of
T14854 2364-2374 RB denotes homologous
T14855 2375-2388 NN denotes recombination
T14856 2388-2389 . denotes .
T14857 2390-2400 JJ denotes Homologous
T14858 2401-2414 NN denotes recombination
T14859 2415-2417 IN denotes of
T14860 2418-2421 DT denotes the
T14861 2422-2428 NN denotes vector
T14862 2429-2433 IN denotes with
T14863 2434-2439 JJ denotes Rnf31
T14864 2440-2448 VBD denotes resulted
T14865 2449-2451 IN denotes in
T14866 2452-2453 DT denotes a
T14867 2454-2459 JJ denotes small
T14868 2460-2471 JJ denotes chromosomal
T14869 2472-2480 NN denotes deletion
T14870 2481-2484 CC denotes and
T14871 2485-2488 DT denotes the
T14872 2489-2497 JJ denotes in-frame
T14873 2498-2507 NN denotes insertion
T14874 2508-2510 IN denotes of
T14875 2511-2519 NN denotes neomycin
T14876 2520-2538 NN denotes phosphotransferase
T14877 2539-2540 -LRB- denotes (
T14878 2540-2544 NNP denotes NeoR
T14879 2544-2545 -RRB- denotes )
T14880 2546-2550 IN denotes into
T14881 2551-2554 DT denotes the
T14882 2555-2569 JJ denotes amino-terminal
T14883 2570-2574 NNP denotes HOIP
T14884 2575-2581 NN denotes coding
T14885 2582-2590 NN denotes sequence
T14886 2590-2591 . denotes .
T14887 2592-2593 DT denotes A
T14888 2594-2604 NN denotes diphtheria
T14889 2605-2610 NN denotes toxin
T14890 2611-2612 -LRB- denotes (
T14891 2612-2614 NNP denotes DT
T14892 2614-2615 -RRB- denotes )
T14893 2616-2624 NN denotes cassette
T14894 2625-2627 IN denotes in
T14895 2628-2631 DT denotes the
T14896 2632-2638 NN denotes vector
T14897 2639-2650 VBZ denotes facilitates
T14898 2651-2654 DT denotes the
T14899 2655-2663 JJ denotes negative
T14900 2664-2673 NN denotes selection
T14901 2674-2676 IN denotes of
T14902 2677-2682 NNS denotes cells
T14903 2683-2685 IN denotes in
T14904 2686-2691 WDT denotes which
T14905 2692-2698 JJ denotes random
T14906 2699-2710 JJ denotes chromosomal
T14907 2711-2720 NN denotes insertion
T14908 2721-2723 IN denotes of
T14909 2724-2727 DT denotes the
T14910 2728-2734 NN denotes vector
T14911 2735-2740 VBZ denotes takes
T14912 2741-2746 NN denotes place
T14913 2746-2747 . denotes .
T14914 2748-2752 NNP denotes LoxP
T14915 2753-2762 NNS denotes sequences
T14916 2763-2768 VBP denotes allow
T14917 2769-2772 DT denotes the
T14918 2773-2785 JJ denotes Cre-mediated
T14919 2786-2794 NN denotes deletion
T14920 2795-2797 IN denotes of
T14921 2798-2801 DT denotes the
T14922 2802-2806 NNP denotes NeoR
T14923 2807-2813 NN denotes coding
T14924 2814-2822 NN denotes sequence
T14925 2822-2823 . denotes .
T14926 2824-2827 DT denotes The
T14927 2828-2834 NNP denotes SV40pA
T14928 2835-2843 NN denotes sequence
T14929 2844-2849 VBZ denotes helps
T14930 2850-2852 TO denotes to
T14931 2853-2859 VB denotes ensure
T14932 2860-2870 NN denotes disruption
T14933 2871-2873 IN denotes of
T14934 2874-2878 NN denotes gene
T14935 2879-2889 NN denotes expression
T14936 2890-2895 IN denotes after
T14937 2896-2904 NN denotes deletion
T14938 2905-2907 IN denotes of
T14939 2908-2911 DT denotes the
T14940 2912-2916 NNP denotes NeoR
T14941 2917-2925 NN denotes sequence
T14942 2925-2926 . denotes .
T14943 2927-2928 -LRB- denotes (
T14944 2928-2929 NNP denotes B
T14945 2929-2930 -RRB- denotes )
T14946 2931-2940 NNP denotes Anti-HOIP
T14947 2941-2944 CC denotes and
T14948 2945-2954 NNP denotes anti-FLAG
T14949 2955-2962 NNP denotes Western
T14950 2963-2968 NNS denotes blots
T14951 2969-2971 IN denotes of
T14952 2972-2976 NN denotes cell
T14953 2977-2984 NNS denotes lysates
T14954 2985-2989 IN denotes from
T14955 2990-2993 NNP denotes A20
T14956 2993-2995 CD denotes .2
T14957 2995-2996 NNP denotes J
T14958 2997-3002 NNS denotes cells
T14959 3003-3006 CC denotes and
T14960 3007-3021 JJ denotes HOIP-deficient
T14961 3022-3023 -LRB- denotes (
T14962 3023-3027 SYM denotes HOIP
T14963 3027-3028 : denotes -
T14964 3028-3029 SYM denotes /
T14965 3029-3030 : denotes -
T14966 3030-3031 -RRB- denotes )
T14967 3032-3038 NNS denotes clones
T14968 3038-3039 . denotes .
T14969 3040-3041 DT denotes A
T14970 3042-3049 JJ denotes partial
T14971 3050-3058 NN denotes decrease
T14972 3059-3061 IN denotes in
T14973 3062-3066 NNP denotes HOIP
T14974 3067-3074 NN denotes protein
T14975 3075-3085 NN denotes expression
T14976 3086-3088 VBZ denotes is
T14977 3089-3096 JJ denotes evident
T14978 3097-3099 IN denotes in
T14979 3100-3105 NNS denotes cells
T14980 3106-3115 VBG denotes following
T14981 3116-3126 NN denotes disruption
T14982 3127-3129 IN denotes of
T14983 3130-3133 CD denotes one
T14984 3134-3138 NN denotes copy
T14985 3139-3141 IN denotes of
T14986 3142-3147 NNP denotes Rnf31
T14987 3148-3149 -LRB- denotes (
T14988 3149-3153 NNP denotes HOIP
T14989 3153-3154 CD denotes −
T14990 3154-3155 CD denotes /
T14991 3155-3156 NN denotes +
T14992 3156-3157 -RRB- denotes )
T14993 3157-3158 . denotes .
T14994 3159-3163 NNP denotes HOIP
T14995 3164-3174 NN denotes expression
T14996 3175-3177 IN denotes in
T14997 3178-3184 NNS denotes clones
T14998 3185-3198 VBN denotes reconstituted
T14999 3199-3203 IN denotes with
T15000 3204-3206 DT denotes an
T15001 3207-3212 JJ denotes empty
T15002 3213-3223 NN denotes retroviral
T15003 3224-3230 NN denotes vector
T15004 3231-3232 -LRB- denotes (
T15005 3232-3236 NNP denotes pMIP
T15006 3236-3237 -RRB- denotes )
T15007 3238-3240 CC denotes or
T15008 3241-3242 DT denotes a
T15009 3243-3253 JJ denotes retroviral
T15010 3254-3260 NN denotes vector
T15011 3261-3269 VBG denotes encoding
T15012 3270-3281 JJ denotes FLAG-tagged
T15013 3282-3286 NNP denotes HOIP
T15014 3287-3289 VBZ denotes is
T15015 3290-3294 RB denotes also
T15016 3295-3300 VBN denotes shown
T15017 3300-3301 . denotes .
T15018 3302-3313 JJ denotes Approximate
T15019 3314-3323 JJ denotes molecular
T15020 3324-3330 NN denotes weight
T15021 3331-3333 IN denotes of
T15022 3334-3338 NNP denotes HOIP
T15023 3339-3341 VBZ denotes is
T15024 3342-3345 CD denotes 120
T15025 3346-3348 NN denotes kD
T15026 3348-3349 . denotes .
R2125 T2880 T2880 ROOT Generation,Generation
R2126 T2881 T2880 prep of,Generation
R2127 T2882 T2883 compound HOIP-deficient,B
R2128 T2883 T2884 compound B,cells
R2129 T2884 T2881 pobj cells,of
R2130 T2885 T2880 prep via,Generation
R2131 T2886 T2887 amod targeted,disruption
R2132 T2887 T2885 pobj disruption,via
R2133 T2888 T2887 prep of,disruption
R2134 T2889 T2888 pobj Rnf31,of
R2135 T2890 T2889 punct ",",Rnf31
R2136 T2891 T2892 det the,gene
R2137 T2892 T2889 appos gene,Rnf31
R2138 T2893 T2892 acl encoding,gene
R2139 T2894 T2893 dobj HOIP,encoding
R2140 T2895 T2901 prep In,demonstrated
R2141 T2896 T2898 poss our,study
R2142 T2897 T2898 amod previous,study
R2143 T2898 T2895 pobj study,In
R2144 T2899 T2901 punct ",",demonstrated
R2145 T2900 T2901 nsubj we,demonstrated
R2146 T2901 T2901 ROOT demonstrated,demonstrated
R2147 T2902 T2905 mark that,recruited
R2148 T2903 T2905 nsubjpass HOIP,recruited
R2149 T2904 T2905 auxpass is,recruited
R2150 T2905 T2901 ccomp recruited,demonstrated
R2151 T2906 T2905 prep to,recruited
R2152 T2907 T2908 det the,CD40
R2153 T2908 T2909 nsubj CD40,signaling
R2154 T2909 T2906 pcomp signaling,to
R2155 T2910 T2909 dobj complex,signaling
R2156 T2911 T2909 prep in,signaling
R2157 T2912 T2916 nummod two,lines
R2158 T2913 T2915 compound mouse,cell
R2159 T2914 T2915 compound B,cell
R2160 T2915 T2916 compound cell,lines
R2161 T2916 T2911 pobj lines,in
R2162 T2917 T2906 pobj [,to
R2163 T2918 T2919 nummod 6,]
R2164 T2919 T2906 pobj ],to
R2165 T2920 T2901 punct .,demonstrated
R2166 T2921 T2922 amod Similar,results
R2167 T2922 T2924 nsubjpass results,obtained
R2168 T2923 T2924 auxpass were,obtained
R2169 T2924 T2924 ROOT obtained,obtained
R2170 T2925 T2924 prep with,obtained
R2171 T2926 T2927 compound mouse,splenocytes
R2172 T2927 T2925 pobj splenocytes,with
R2173 T2928 T2924 punct ",",obtained
R2174 T2929 T2924 advcl indicating,obtained
R2175 T2930 T2939 mark that,limited
R2176 T2931 T2932 det the,interaction
R2177 T2932 T2939 nsubjpass interaction,limited
R2178 T2933 T2932 prep of,interaction
R2179 T2934 T2933 pobj CD40,of
R2180 T2935 T2932 prep with,interaction
R2181 T2936 T2935 pobj HOIP,with
R2182 T2937 T2939 auxpass is,limited
R2183 T2938 T2939 neg not,limited
R2184 T2939 T2929 ccomp limited,indicating
R2185 T2940 T2941 aux to,transformed
R2186 T2941 T2939 xcomp transformed,limited
R2187 T2942 T2943 compound B,cell
R2188 T2943 T2944 compound cell,lines
R2189 T2944 T2941 dobj lines,transformed
R2190 T2945 T2944 punct (,lines
R2191 T2946 T2947 amod unpublished,observations
R2192 T2947 T2944 appos observations,lines
R2193 T2948 T2944 punct ),lines
R2194 T2949 T2924 punct .,obtained
R2195 T2950 T2952 nsubj We,found
R2196 T2951 T2952 advmod also,found
R2197 T2952 T2952 ROOT found,found
R2198 T2953 T2960 mark that,was
R2199 T2954 T2955 det the,recruitment
R2200 T2955 T2960 nsubj recruitment,was
R2201 T2956 T2955 prep of,recruitment
R2202 T2957 T2956 pobj HOIP,of
R2203 T2958 T2955 prep to,recruitment
R2204 T2959 T2958 pobj CD40,to
R2205 T2960 T2952 ccomp was,found
R2206 T2961 T2960 acomp TRAF2-dependent,was
R2207 T2962 T2960 cc and,was
R2208 T2963 T2964 det that,overexpression
R2209 T2964 T2960 conj overexpression,was
R2210 T2965 T2964 prep of,overexpression
R2211 T2966 T2969 det a,mutant
R2212 T2967 T2969 amod truncated,mutant
R2213 T2968 T2969 compound HOIP,mutant
R2214 T2969 T2974 amod mutant,activation
R2215 T2970 T2971 advmod partially,inhibited
R2216 T2971 T2974 amod inhibited,activation
R2217 T2972 T2974 amod CD40-mediated,activation
R2218 T2973 T2974 compound NF-κB,activation
R2219 T2974 T2965 pobj activation,of
R2220 T2975 T2952 punct .,found
R2221 T2976 T2977 det These,results
R2222 T2977 T2978 nsubj results,support
R2223 T2978 T2978 ROOT support,support
R2224 T2979 T2980 det the,hypothesis
R2225 T2980 T2978 dobj hypothesis,support
R2226 T2981 T2983 mark that,plays
R2227 T2982 T2983 nsubj HOIP,plays
R2228 T2983 T2980 acl plays,hypothesis
R2229 T2984 T2985 det a,role
R2230 T2985 T2983 dobj role,plays
R2231 T2986 T2985 prep in,role
R2232 T2987 T2989 nummod CD40,transduction
R2233 T2988 T2989 compound signal,transduction
R2234 T2989 T2986 pobj transduction,in
R2235 T2990 T2978 punct .,support
R2236 T2991 T2993 aux To,define
R2237 T2992 T2993 advmod further,define
R2238 T2993 T3007 advcl define,used
R2239 T2994 T2995 det the,role
R2240 T2995 T2993 dobj role,define
R2241 T2996 T2993 cc and,define
R2242 T2997 T2993 conj evaluate,define
R2243 T2998 T2999 det the,importance
R2244 T2999 T2997 dobj importance,evaluate
R2245 T3000 T2999 prep of,importance
R2246 T3001 T3000 pobj HOIP,of
R2247 T3002 T2997 prep in,evaluate
R2248 T3003 T3002 pobj CD40,in
R2249 T3004 T2997 advcl signaling,evaluate
R2250 T3005 T3007 punct ",",used
R2251 T3006 T3007 nsubj we,used
R2252 T3007 T3007 ROOT used,used
R2253 T3008 T3010 compound somatic,gene
R2254 T3009 T3010 compound cell,gene
R2255 T3010 T3007 dobj gene,used
R2256 T3011 T3010 acl targeting,gene
R2257 T3012 T3013 aux to,disrupt
R2258 T3013 T3011 xcomp disrupt,targeting
R2259 T3014 T3015 det the,gene
R2260 T3015 T3013 dobj gene,disrupt
R2261 T3016 T3015 acl encoding,gene
R2262 T3017 T3016 dobj HOIP,encoding
R2263 T3018 T3016 prep in,encoding
R2264 T3019 T3023 det the,line
R2265 T3020 T3022 compound mouse,cell
R2266 T3021 T3022 compound B,cell
R2267 T3022 T3023 compound cell,line
R2268 T3023 T3018 pobj line,in
R2269 T3024 T3026 nmod A20,J
R2270 T3025 T3026 nummod .2,J
R2271 T3026 T3023 appos J,line
R2272 T3027 T3007 punct .,used
R2273 T3028 T3030 det This,line
R2274 T3029 T3030 compound cell,line
R2275 T3030 T3032 nsubj line,been
R2276 T3031 T3032 aux has,been
R2277 T3032 T3032 ROOT been,been
R2278 T3033 T3034 advmod particularly,useful
R2279 T3034 T3032 acomp useful,been
R2280 T3035 T3034 prep in,useful
R2281 T3036 T3037 det the,characterization
R2282 T3037 T3035 pobj characterization,in
R2283 T3038 T3037 prep of,characterization
R2284 T3039 T3038 pobj CD40,of
R2285 T3040 T3032 advcl signaling,been
R2286 T3041 T3040 dobj mechanisms,signaling
R2287 T3042 T3041 amod due,mechanisms
R2288 T3043 T3042 pcomp to,due
R2289 T3044 T3046 det the,ease
R2290 T3045 T3046 amod relative,ease
R2291 T3046 T3042 pobj ease,due
R2292 T3047 T3053 prep with,modified
R2293 T3048 T3047 pobj which,with
R2294 T3049 T3050 poss its,genome
R2295 T3050 T3053 nsubjpass genome,modified
R2296 T3051 T3053 aux can,modified
R2297 T3052 T3053 auxpass be,modified
R2298 T3053 T3046 relcl modified,ease
R2299 T3054 T3053 agent by,modified
R2300 T3055 T3057 nmod homologous,[
R2301 T3056 T3057 compound recombination,[
R2302 T3057 T3059 nmod [,]
R2303 T3058 T3059 nummod 7,]
R2304 T3059 T3054 pobj ],by
R2305 T3060 T3059 punct ",",]
R2306 T3061 T3063 nmod [,]
R2307 T3062 T3063 nummod 8,]
R2308 T3063 T3059 conj ],]
R2309 T3064 T3063 punct ",",]
R2310 T3065 T3067 det [,]
R2311 T3066 T3067 nummod 9,]
R2312 T3067 T3063 appos ],]
R2313 T3068 T3032 punct .,been
R2314 T3069 T3070 nsubj We,used
R2315 T3070 T3070 ROOT used,used
R2316 T3071 T3073 det a,vector
R2317 T3072 T3073 compound targeting,vector
R2318 T3073 T3070 dobj vector,used
R2319 T3074 T3073 amod capable,vector
R2320 T3075 T3074 prep of,capable
R2321 T3076 T3075 pcomp undergoing,of
R2322 T3077 T3078 amod homologous,recombination
R2323 T3078 T3076 dobj recombination,undergoing
R2324 T3079 T3076 prep with,undergoing
R2325 T3080 T3079 pobj Rnf31,with
R2326 T3081 T3080 punct (,Rnf31
R2327 T3082 T3083 det the,gene
R2328 T3083 T3080 appos gene,Rnf31
R2329 T3084 T3083 acl encoding,gene
R2330 T3085 T3084 dobj HOIP,encoding
R2331 T3086 T3080 punct ),Rnf31
R2332 T3087 T3088 aux to,disrupt
R2333 T3088 T3076 advcl disrupt,undergoing
R2334 T3089 T3091 det the,sequence
R2335 T3090 T3091 compound coding,sequence
R2336 T3091 T3088 dobj sequence,disrupt
R2337 T3092 T3091 prep of,sequence
R2338 T3093 T3094 det the,gene
R2339 T3094 T3092 pobj gene,of
R2340 T3095 T3088 prep in,disrupt
R2341 T3096 T3095 pobj exon,in
R2342 T3097 T3096 nummod 5,exon
R2343 T3098 T3096 punct (,exon
R2344 T3099 T3100 compound Fig.,1A
R2345 T3100 T3096 appos 1A,exon
R2346 T3101 T3096 punct ),exon
R2347 T3102 T3070 punct .,used
R2348 T3103 T3115 prep Following,screened
R2349 T3104 T3103 pobj introduction,Following
R2350 T3105 T3104 prep of,introduction
R2351 T3106 T3107 det the,vector
R2352 T3107 T3105 pobj vector,of
R2353 T3108 T3115 punct ",",screened
R2354 T3109 T3111 det the,clones
R2355 T3110 T3111 amod neomycin-resistant,clones
R2356 T3111 T3115 nsubjpass clones,screened
R2357 T3112 T3113 nsubj that,arose
R2358 T3113 T3111 relcl arose,clones
R2359 T3114 T3115 auxpass were,screened
R2360 T3115 T3115 ROOT screened,screened
R2361 T3116 T3115 agent by,screened
R2362 T3117 T3118 compound PCR,amplification
R2363 T3118 T3116 pobj amplification,by
R2364 T3119 T3118 prep of,amplification
R2365 T3120 T3121 amod genomic,DNA
R2366 T3121 T3119 pobj DNA,of
R2367 T3122 T3123 aux to,identify
R2368 T3123 T3115 xcomp identify,screened
R2369 T3124 T3123 dobj cells,identify
R2370 T3125 T3124 acl containing,cells
R2371 T3126 T3129 det a,allele
R2372 T3127 T3129 amod disrupted,allele
R2373 T3128 T3129 compound Rnf31,allele
R2374 T3129 T3125 dobj allele,containing
R2375 T3130 T3115 punct .,screened
R2376 T3131 T3132 aux To,remove
R2377 T3132 T3149 advcl remove,transfected
R2378 T3133 T3137 det the,cassette
R2379 T3134 T3136 amod selectable,gene
R2380 T3135 T3136 compound marker,gene
R2381 T3136 T3137 compound gene,cassette
R2382 T3137 T3132 dobj cassette,remove
R2383 T3138 T3132 prep from,remove
R2384 T3139 T3142 det the,allele
R2385 T3140 T3142 amod disrupted,allele
R2386 T3141 T3142 compound Rnf31,allele
R2387 T3142 T3138 pobj allele,from
R2388 T3143 T3149 punct ",",transfected
R2389 T3144 T3146 amod recombinant,lines
R2390 T3145 T3146 compound cell,lines
R2391 T3146 T3149 nsubjpass lines,transfected
R2392 T3147 T3149 auxpass were,transfected
R2393 T3148 T3149 advmod transiently,transfected
R2394 T3149 T3149 ROOT transfected,transfected
R2395 T3150 T3149 prep with,transfected
R2396 T3151 T3152 det a,plasmid
R2397 T3152 T3150 pobj plasmid,with
R2398 T3153 T3154 nsubj that,encodes
R2399 T3154 T3152 relcl encodes,plasmid
R2400 T3155 T3156 compound Cre,recombinase
R2401 T3156 T3154 dobj recombinase,encodes
R2402 T3157 T3149 punct .,transfected
R2403 T3158 T3159 det This,step
R2404 T3159 T3160 nsubj step,allowed
R2405 T3160 T3160 ROOT allowed,allowed
R2406 T3161 T3163 nsubj us,perform
R2407 T3162 T3163 aux to,perform
R2408 T3163 T3160 ccomp perform,allowed
R2409 T3164 T3166 det a,round
R2410 T3165 T3166 amod second,round
R2411 T3166 T3163 dobj round,perform
R2412 T3167 T3166 prep of,round
R2413 T3168 T3167 pobj targeting,of
R2414 T3169 T3168 cc and,targeting
R2415 T3170 T3171 compound drug,selection
R2416 T3171 T3168 conj selection,targeting
R2417 T3172 T3171 punct ",",selection
R2418 T3173 T3168 conj generating,targeting
R2419 T3174 T3173 dobj cells,generating
R2420 T3175 T3182 prep in,disrupted
R2421 T3176 T3175 pobj which,in
R2422 T3177 T3178 det both,copies
R2423 T3178 T3182 nsubjpass copies,disrupted
R2424 T3179 T3178 prep of,copies
R2425 T3180 T3179 pobj Rnf31,of
R2426 T3181 T3182 auxpass were,disrupted
R2427 T3182 T3174 relcl disrupted,cells
R2428 T3183 T3160 punct .,allowed
R2429 T3184 T3188 nummod Two,lines
R2430 T3185 T3188 amod independent,lines
R2431 T3186 T3188 amod clonal,lines
R2432 T3187 T3188 compound cell,lines
R2433 T3188 T3190 nsubjpass lines,chosen
R2434 T3189 T3190 auxpass were,chosen
R2435 T3190 T3190 ROOT chosen,chosen
R2436 T3191 T3190 prep for,chosen
R2437 T3192 T3193 amod further,analysis
R2438 T3193 T3191 pobj analysis,for
R2439 T3194 T3190 punct .,chosen
R2440 T3195 T3197 compound HOIP,expression
R2441 T3196 T3197 compound protein,expression
R2442 T3197 T3198 nsubj expression,was
R2443 T3198 T3198 ROOT was,was
R2444 T3199 T3198 acomp undetectable,was
R2445 T3200 T3199 prep in,undetectable
R2446 T3201 T3203 det both,lines
R2447 T3202 T3203 compound cell,lines
R2448 T3203 T3200 pobj lines,in
R2449 T3204 T3198 punct ",",was
R2450 T3205 T3206 mark as,determined
R2451 T3206 T3198 advcl determined,was
R2452 T3207 T3206 agent by,determined
R2453 T3208 T3210 amod Western,analysis
R2454 T3209 T3210 compound blot,analysis
R2455 T3210 T3207 pobj analysis,by
R2456 T3211 T3210 prep of,analysis
R2457 T3212 T3213 compound cell,lysates
R2458 T3213 T3211 pobj lysates,of
R2459 T3214 T3210 punct (,analysis
R2460 T3215 T3216 compound Fig.,1B
R2461 T3216 T3210 appos 1B,analysis
R2462 T3217 T3210 punct ),analysis
R2463 T3218 T3198 punct ",",was
R2464 T3219 T3198 advcl demonstrating,was
R2465 T3220 T3224 mark that,was
R2466 T3221 T3223 det the,process
R2467 T3222 T3223 compound targeting,process
R2468 T3223 T3224 nsubj process,was
R2469 T3224 T3219 ccomp was,demonstrating
R2470 T3225 T3224 acomp successful,was
R2471 T3226 T3198 punct .,was
R2472 T3227 T3239 prep In,referred
R2473 T3228 T3229 det the,text
R2474 T3229 T3227 pobj text,In
R2475 T3230 T3231 nsubj that,follows
R2476 T3231 T3229 relcl follows,text
R2477 T3232 T3239 punct ",",referred
R2478 T3233 T3237 det the,lines
R2479 T3234 T3237 nummod two,lines
R2480 T3235 T3237 amod gene-targeted,lines
R2481 T3236 T3237 compound cell,lines
R2482 T3237 T3239 nsubjpass lines,referred
R2483 T3238 T3239 auxpass are,referred
R2484 T3239 T3239 ROOT referred,referred
R2485 T3240 T3239 prep to,referred
R2486 T3241 T3239 prep as,referred
R2487 T3242 T3243 amod HOIP-deficient,cells
R2488 T3243 T3241 pobj cells,as
R2489 T3244 T3239 punct .,referred
R2490 T3245 T3246 aux To,enable
R2491 T3246 T3274 advcl enable,transduced
R2492 T3247 T3246 dobj us,enable
R2493 T3248 T3249 aux to,confirm
R2494 T3249 T3246 xcomp confirm,enable
R2495 T3250 T3260 mark that,were
R2496 T3251 T3252 det any,signaling
R2497 T3252 T3260 nsubj signaling,were
R2498 T3253 T3252 cc or,signaling
R2499 T3254 T3255 amod functional,defects
R2500 T3255 T3256 nsubj defects,observed
R2501 T3256 T3252 conj observed,signaling
R2502 T3257 T3256 prep in,observed
R2503 T3258 T3259 amod HOIP-deficient,cells
R2504 T3259 T3257 pobj cells,in
R2505 T3260 T3249 ccomp were,confirm
R2506 T3261 T3260 acomp due,were
R2507 T3262 T3261 prep to,due
R2508 T3263 T3262 pobj disruption,to
R2509 T3264 T3263 prep of,disruption
R2510 T3265 T3267 det the,gene
R2511 T3266 T3267 compound Rnf31,gene
R2512 T3267 T3264 pobj gene,of
R2513 T3268 T3274 punct ",",transduced
R2514 T3269 T3272 det both,lines
R2515 T3270 T3272 amod HOIP-deficient,lines
R2516 T3271 T3272 compound cell,lines
R2517 T3272 T3274 nsubjpass lines,transduced
R2518 T3273 T3274 auxpass were,transduced
R2519 T3274 T3274 ROOT transduced,transduced
R2520 T3275 T3274 prep with,transduced
R2521 T3276 T3277 det a,retrovirus
R2522 T3277 T3278 nsubj retrovirus,encoding
R2523 T3278 T3281 amod encoding,HOIP
R2524 T3279 T3281 compound FLAG-tagged,HOIP
R2525 T3280 T3281 compound wild-type,HOIP
R2526 T3281 T3275 pobj HOIP,with
R2527 T3282 T3274 punct ",",transduced
R2528 T3283 T3284 advmod thus,restoring
R2529 T3284 T3274 advcl restoring,transduced
R2530 T3285 T3287 compound HOIP,expression
R2531 T3286 T3287 compound protein,expression
R2532 T3287 T3284 dobj expression,restoring
R2533 T3288 T3290 punct (,1B
R2534 T3289 T3290 compound Fig.,1B
R2535 T3290 T3284 appos 1B,restoring
R2536 T3291 T3290 punct ),1B
R2537 T3292 T3274 punct .,transduced
R2538 T3293 T3294 det These,cells
R2539 T3294 T3296 nsubjpass cells,referred
R2540 T3295 T3296 auxpass are,referred
R2541 T3296 T3296 ROOT referred,referred
R2542 T3297 T3296 prep to,referred
R2543 T3298 T3296 prep as,referred
R2544 T3299 T3300 amod HOIP-reconstituted,cells
R2545 T3300 T3298 pobj cells,as
R2546 T3301 T3296 punct .,referred
R2547 T3302 T3313 prep In,compared
R2548 T3303 T3304 det the,experiments
R2549 T3304 T3302 pobj experiments,In
R2550 T3305 T3306 nsubj that,follow
R2551 T3306 T3304 relcl follow,experiments
R2552 T3307 T3313 punct ",",compared
R2553 T3308 T3313 nsubjpass responses,compared
R2554 T3309 T3308 prep by,responses
R2555 T3310 T3311 det these,cells
R2556 T3311 T3309 pobj cells,by
R2557 T3312 T3313 auxpass were,compared
R2558 T3313 T3313 ROOT compared,compared
R2559 T3314 T3313 prep to,compared
R2560 T3315 T3314 pobj those,to
R2561 T3316 T3315 prep of,those
R2562 T3317 T3320 compound A20,cells
R2563 T3318 T3319 compound .2,J
R2564 T3319 T3320 compound J,cells
R2565 T3320 T3316 pobj cells,of
R2566 T3321 T3320 cc and,cells
R2567 T3322 T3323 amod HOIP-deficient,cells
R2568 T3323 T3320 conj cells,cells
R2569 T3324 T3315 acl transduced,those
R2570 T3325 T3324 prep with,transduced
R2571 T3326 T3328 det a,vector
R2572 T3327 T3328 amod retroviral,vector
R2573 T3328 T3325 pobj vector,with
R2574 T3329 T3328 punct (,vector
R2575 T3330 T3328 appos pMIP,vector
R2576 T3331 T3328 punct ),vector
R2577 T3332 T3315 acl lacking,those
R2578 T3333 T3335 det a,insert
R2579 T3334 T3335 compound cDNA,insert
R2580 T3335 T3332 dobj insert,lacking
R2581 T3336 T3335 punct (,insert
R2582 T3337 T3338 amod empty,vector
R2583 T3338 T3335 appos vector,insert
R2584 T3339 T3335 punct ),insert
R2585 T3340 T3313 punct .,compared
R11703 T14807 T14808 compound HOIP,gene
R11704 T14808 T14809 compound gene,targeting
R11705 T14809 T14809 ROOT targeting,targeting
R11706 T14810 T14809 punct .,targeting
R11707 T14811 T14833 punct (,shown
R11708 T14812 T14833 advmod A,shown
R11709 T14813 T14814 punct ),Regions
R11710 T14814 T14833 nsubjpass Regions,shown
R11711 T14815 T14814 prep of,Regions
R11712 T14816 T14818 amod Rnf31,sequence
R11713 T14817 T14818 compound gene,sequence
R11714 T14818 T14815 pobj sequence,of
R11715 T14819 T14818 punct (,sequence
R11716 T14820 T14818 appos bottom,sequence
R11717 T14821 T14818 punct ),sequence
R11718 T14822 T14814 acl used,Regions
R11719 T14823 T14822 prep in,used
R11720 T14824 T14826 det the,gene
R11721 T14825 T14826 compound HOIP,gene
R11722 T14826 T14823 pobj gene,in
R11723 T14827 T14814 acl targeting,Regions
R11724 T14828 T14827 dobj vector,targeting
R11725 T14829 T14828 punct (,vector
R11726 T14830 T14828 amod top,vector
R11727 T14831 T14814 punct ),Regions
R11728 T14832 T14833 auxpass are,shown
R11729 T14833 T14833 ROOT shown,shown
R11730 T14834 T14833 punct .,shown
R11731 T14835 T14841 nsubj Arrows,indicate
R11732 T14836 T14837 punct (,F
R11733 T14837 T14835 appos F,Arrows
R11734 T14838 T14837 cc and,F
R11735 T14839 T14837 conj R,F
R11736 T14840 T14835 punct ),Arrows
R11737 T14841 T14841 ROOT indicate,indicate
R11738 T14842 T14843 amod approximate,positions
R11739 T14843 T14841 dobj positions,indicate
R11740 T14844 T14843 prep of,positions
R11741 T14845 T14844 pobj sequences,of
R11742 T14846 T14841 advmod homologous,indicate
R11743 T14847 T14846 prep to,homologous
R11744 T14848 T14847 pobj oligonucleotides,to
R11745 T14849 T14848 acl used,oligonucleotides
R11746 T14850 T14849 prep for,used
R11747 T14851 T14852 amod PCR-mediated,detection
R11748 T14852 T14850 pobj detection,for
R11749 T14853 T14852 prep of,detection
R11750 T14854 T14855 amod homologous,recombination
R11751 T14855 T14853 pobj recombination,of
R11752 T14856 T14841 punct .,indicate
R11753 T14857 T14858 amod Homologous,recombination
R11754 T14858 T14864 nsubj recombination,resulted
R11755 T14859 T14858 prep of,recombination
R11756 T14860 T14861 det the,vector
R11757 T14861 T14859 pobj vector,of
R11758 T14862 T14858 prep with,recombination
R11759 T14863 T14862 pobj Rnf31,with
R11760 T14864 T14864 ROOT resulted,resulted
R11761 T14865 T14864 prep in,resulted
R11762 T14866 T14869 det a,deletion
R11763 T14867 T14869 amod small,deletion
R11764 T14868 T14869 amod chromosomal,deletion
R11765 T14869 T14865 pobj deletion,in
R11766 T14870 T14869 cc and,deletion
R11767 T14871 T14873 det the,insertion
R11768 T14872 T14873 amod in-frame,insertion
R11769 T14873 T14869 conj insertion,deletion
R11770 T14874 T14873 prep of,insertion
R11771 T14875 T14876 compound neomycin,phosphotransferase
R11772 T14876 T14874 pobj phosphotransferase,of
R11773 T14877 T14878 punct (,NeoR
R11774 T14878 T14876 appos NeoR,phosphotransferase
R11775 T14879 T14864 punct ),resulted
R11776 T14880 T14864 prep into,resulted
R11777 T14881 T14885 det the,sequence
R11778 T14882 T14885 amod amino-terminal,sequence
R11779 T14883 T14885 compound HOIP,sequence
R11780 T14884 T14885 compound coding,sequence
R11781 T14885 T14880 pobj sequence,into
R11782 T14886 T14864 punct .,resulted
R11783 T14887 T14889 det A,toxin
R11784 T14888 T14889 compound diphtheria,toxin
R11785 T14889 T14897 nsubj toxin,facilitates
R11786 T14890 T14889 punct (,toxin
R11787 T14891 T14889 appos DT,toxin
R11788 T14892 T14889 punct ),toxin
R11789 T14893 T14889 appos cassette,toxin
R11790 T14894 T14893 prep in,cassette
R11791 T14895 T14896 det the,vector
R11792 T14896 T14894 pobj vector,in
R11793 T14897 T14897 ROOT facilitates,facilitates
R11794 T14898 T14900 det the,selection
R11795 T14899 T14900 amod negative,selection
R11796 T14900 T14897 dobj selection,facilitates
R11797 T14901 T14900 prep of,selection
R11798 T14902 T14901 pobj cells,of
R11799 T14903 T14911 prep in,takes
R11800 T14904 T14903 pobj which,in
R11801 T14905 T14907 amod random,insertion
R11802 T14906 T14907 amod chromosomal,insertion
R11803 T14907 T14911 nsubj insertion,takes
R11804 T14908 T14907 prep of,insertion
R11805 T14909 T14910 det the,vector
R11806 T14910 T14908 pobj vector,of
R11807 T14911 T14900 relcl takes,selection
R11808 T14912 T14911 dobj place,takes
R11809 T14913 T14897 punct .,facilitates
R11810 T14914 T14915 compound LoxP,sequences
R11811 T14915 T14916 nsubj sequences,allow
R11812 T14916 T14916 ROOT allow,allow
R11813 T14917 T14919 det the,deletion
R11814 T14918 T14919 amod Cre-mediated,deletion
R11815 T14919 T14916 dobj deletion,allow
R11816 T14920 T14919 prep of,deletion
R11817 T14921 T14924 det the,sequence
R11818 T14922 T14924 compound NeoR,sequence
R11819 T14923 T14924 compound coding,sequence
R11820 T14924 T14920 pobj sequence,of
R11821 T14925 T14916 punct .,allow
R11822 T14926 T14928 det The,sequence
R11823 T14927 T14928 compound SV40pA,sequence
R11824 T14928 T14929 nsubj sequence,helps
R11825 T14929 T14929 ROOT helps,helps
R11826 T14930 T14931 aux to,ensure
R11827 T14931 T14929 xcomp ensure,helps
R11828 T14932 T14931 dobj disruption,ensure
R11829 T14933 T14932 prep of,disruption
R11830 T14934 T14935 compound gene,expression
R11831 T14935 T14933 pobj expression,of
R11832 T14936 T14931 prep after,ensure
R11833 T14937 T14936 pobj deletion,after
R11834 T14938 T14937 prep of,deletion
R11835 T14939 T14941 det the,sequence
R11836 T14940 T14941 compound NeoR,sequence
R11837 T14941 T14938 pobj sequence,of
R11838 T14942 T14929 punct .,helps
R11839 T14943 T14946 punct (,Anti-HOIP
R11840 T14944 T14946 nmod B,Anti-HOIP
R11841 T14945 T14946 punct ),Anti-HOIP
R11842 T14946 T14950 nmod Anti-HOIP,blots
R11843 T14947 T14946 cc and,Anti-HOIP
R11844 T14948 T14949 compound anti-FLAG,Western
R11845 T14949 T14946 conj Western,Anti-HOIP
R11846 T14950 T14950 ROOT blots,blots
R11847 T14951 T14950 prep of,blots
R11848 T14952 T14953 compound cell,lysates
R11849 T14953 T14951 pobj lysates,of
R11850 T14954 T14950 prep from,blots
R11851 T14955 T14958 compound A20,cells
R11852 T14956 T14957 compound .2,J
R11853 T14957 T14958 compound J,cells
R11854 T14958 T14954 pobj cells,from
R11855 T14959 T14958 cc and,cells
R11856 T14960 T14958 conj HOIP-deficient,cells
R11857 T14961 T14967 punct (,clones
R11858 T14962 T14967 dep HOIP,clones
R11859 T14963 T14967 punct -,clones
R11860 T14964 T14967 amod /,clones
R11861 T14965 T14964 punct -,/
R11862 T14966 T14964 punct ),/
R11863 T14967 T14958 conj clones,cells
R11864 T14968 T14950 punct .,blots
R11865 T14969 T14971 det A,decrease
R11866 T14970 T14971 amod partial,decrease
R11867 T14971 T14976 nsubj decrease,is
R11868 T14972 T14971 prep in,decrease
R11869 T14973 T14975 compound HOIP,expression
R11870 T14974 T14975 compound protein,expression
R11871 T14975 T14972 pobj expression,in
R11872 T14976 T14976 ROOT is,is
R11873 T14977 T14976 acomp evident,is
R11874 T14978 T14976 prep in,is
R11875 T14979 T14978 pobj cells,in
R11876 T14980 T14979 acl following,cells
R11877 T14981 T14980 dobj disruption,following
R11878 T14982 T14981 prep of,disruption
R11879 T14983 T14984 nummod one,copy
R11880 T14984 T14982 pobj copy,of
R11881 T14985 T14984 prep of,copy
R11882 T14986 T14985 pobj Rnf31,of
R11883 T14987 T14986 punct (,Rnf31
R11884 T14988 T14990 neg HOIP,/
R11885 T14989 T14990 compound −,/
R11886 T14990 T14986 parataxis /,Rnf31
R11887 T14991 T14986 appos +,Rnf31
R11888 T14992 T14986 punct ),Rnf31
R11889 T14993 T14976 punct .,is
R11890 T14994 T14995 compound HOIP,expression
R11891 T14995 T15016 nsubjpass expression,shown
R11892 T14996 T14995 prep in,expression
R11893 T14997 T14996 pobj clones,in
R11894 T14998 T14997 acl reconstituted,clones
R11895 T14999 T14998 prep with,reconstituted
R11896 T15000 T15003 det an,vector
R11897 T15001 T15003 amod empty,vector
R11898 T15002 T15003 compound retroviral,vector
R11899 T15003 T14999 pobj vector,with
R11900 T15004 T15003 punct (,vector
R11901 T15005 T15003 appos pMIP,vector
R11902 T15006 T15003 punct ),vector
R11903 T15007 T15003 cc or,vector
R11904 T15008 T15010 det a,vector
R11905 T15009 T15010 amod retroviral,vector
R11906 T15010 T15003 conj vector,vector
R11907 T15011 T15010 acl encoding,vector
R11908 T15012 T15013 compound FLAG-tagged,HOIP
R11909 T15013 T15011 dobj HOIP,encoding
R11910 T15014 T15016 auxpass is,shown
R11911 T15015 T15016 advmod also,shown
R11912 T15016 T15016 ROOT shown,shown
R11913 T15017 T15016 punct .,shown
R11914 T15018 T15020 amod Approximate,weight
R11915 T15019 T15020 amod molecular,weight
R11916 T15020 T15023 nsubj weight,is
R11917 T15021 T15020 prep of,weight
R11918 T15022 T15021 pobj HOIP,of
R11919 T15023 T15023 ROOT is,is
R11920 T15024 T15025 nummod 120,kD
R11921 T15025 T15023 attr kD,is
R11922 T15026 T15023 punct .,is

GO-BP

Id Subject Object Predicate Lexical cue
T3835 168-177 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T3836 726-735 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T3837 916-925 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T3838 3385-3394 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T3839 634-653 http://purl.obolibrary.org/obo/GO_0007165 denotes signal transduction
T3840 641-653 http://purl.obolibrary.org/obo/GO_0009293 denotes transduction
T15254 2874-2889 http://purl.obolibrary.org/obo/GO_0010467 denotes gene expression

GO-CC

Id Subject Object Predicate Lexical cue
T15257 2460-2471 http://purl.obolibrary.org/obo/GO_0005694 denotes chromosomal
T15258 2699-2710 http://purl.obolibrary.org/obo/GO_0005694 denotes chromosomal
T15259 2677-2682 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15260 2997-3002 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15261 3100-3105 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3841 31-36 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3842 1358-1363 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3843 1668-1673 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3844 2065-2070 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3845 3444-3449 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3846 3650-3655 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3847 3694-3699 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3848 3752-3757 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3849 3791-3796 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3850 3816-3821 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

sentences

Id Subject Object Predicate Lexical cue
T2863 0-93 Sentence denotes Generation of HOIP-deficient B cells via targeted disruption of Rnf31, the gene encoding HOIP
T2864 94-216 Sentence denotes In our previous study, we demonstrated that HOIP is recruited to the CD40 signaling complex in two mouse B cell lines [6].
T2865 217-391 Sentence denotes Similar results were obtained with mouse splenocytes, indicating that the interaction of CD40 with HOIP is not limited to transformed B cell lines (unpublished observations).
T2866 392-565 Sentence denotes We also found that the recruitment of HOIP to CD40 was TRAF2-dependent and that overexpression of a truncated HOIP mutant partially inhibited CD40-mediated NF-κB activation.
T2867 566-654 Sentence denotes These results support the hypothesis that HOIP plays a role in CD40 signal transduction.
T2868 655-839 Sentence denotes To further define the role and evaluate the importance of HOIP in CD40 signaling, we used somatic cell gene targeting to disrupt the gene encoding HOIP in the mouse B cell line A20.2J.
T2869 840-1042 Sentence denotes This cell line has been particularly useful in the characterization of CD40 signaling mechanisms due to the relative ease with which its genome can be modified by homologous recombination [7], [8], [9].
T2870 1043-1216 Sentence denotes We used a targeting vector capable of undergoing homologous recombination with Rnf31 (the gene encoding HOIP) to disrupt the coding sequence of the gene in exon 5 (Fig. 1A).
T2871 1217-1400 Sentence denotes Following introduction of the vector, the neomycin-resistant clones that arose were screened by PCR amplification of genomic DNA to identify cells containing a disrupted Rnf31 allele.
T2872 1401-1576 Sentence denotes To remove the selectable marker gene cassette from the disrupted Rnf31 allele, recombinant cell lines were transiently transfected with a plasmid that encodes Cre recombinase.
T2873 1577-1719 Sentence denotes This step allowed us to perform a second round of targeting and drug selection, generating cells in which both copies of Rnf31 were disrupted.
T2874 1720-1787 Sentence denotes Two independent clonal cell lines were chosen for further analysis.
T2875 1788-1971 Sentence denotes HOIP protein expression was undetectable in both cell lines, as determined by Western blot analysis of cell lysates (Fig. 1B), demonstrating that the targeting process was successful.
T2876 1972-2071 Sentence denotes In the text that follows, the two gene-targeted cell lines are referred to as HOIP-deficient cells.
T2877 3352-3643 Sentence denotes To enable us to confirm that any signaling or functional defects observed in HOIP-deficient cells were due to disruption of the Rnf31 gene, both HOIP-deficient cell lines were transduced with a retrovirus encoding FLAG-tagged wild-type HOIP, thus restoring HOIP protein expression (Fig. 1B).
T2878 3644-3700 Sentence denotes These cells are referred to as HOIP-reconstituted cells.
T2879 3701-3902 Sentence denotes In the experiments that follow, responses by these cells were compared to those of A20.2J cells and HOIP-deficient cells transduced with a retroviral vector (pMIP) lacking a cDNA insert (empty vector).
T14797 2116-2136 Sentence denotes HOIP gene targeting.
T14798 2137-2236 Sentence denotes (A) Regions of Rnf31 gene sequence (bottom) used in the HOIP gene targeting vector (top) are shown.
T14799 2237-2389 Sentence denotes Arrows (F and R) indicate approximate positions of sequences homologous to oligonucleotides used for PCR-mediated detection of homologous recombination.
T14800 2390-2591 Sentence denotes Homologous recombination of the vector with Rnf31 resulted in a small chromosomal deletion and the in-frame insertion of neomycin phosphotransferase (NeoR) into the amino-terminal HOIP coding sequence.
T14801 2592-2747 Sentence denotes A diphtheria toxin (DT) cassette in the vector facilitates the negative selection of cells in which random chromosomal insertion of the vector takes place.
T14802 2748-2823 Sentence denotes LoxP sequences allow the Cre-mediated deletion of the NeoR coding sequence.
T14803 2824-3039 Sentence denotes The SV40pA sequence helps to ensure disruption of gene expression after deletion of the NeoR sequence. (B) Anti-HOIP and anti-FLAG Western blots of cell lysates from A20.2J cells and HOIP-deficient (HOIP-/-) clones.
T14804 3040-3158 Sentence denotes A partial decrease in HOIP protein expression is evident in cells following disruption of one copy of Rnf31 (HOIP−/+).
T14805 3159-3301 Sentence denotes HOIP expression in clones reconstituted with an empty retroviral vector (pMIP) or a retroviral vector encoding FLAG-tagged HOIP is also shown.
T14806 3302-3349 Sentence denotes Approximate molecular weight of HOIP is 120 kD.
T35 0-93 Sentence denotes Generation of HOIP-deficient B cells via targeted disruption of Rnf31, the gene encoding HOIP
T36 94-216 Sentence denotes In our previous study, we demonstrated that HOIP is recruited to the CD40 signaling complex in two mouse B cell lines [6].
T37 217-391 Sentence denotes Similar results were obtained with mouse splenocytes, indicating that the interaction of CD40 with HOIP is not limited to transformed B cell lines (unpublished observations).
T38 392-565 Sentence denotes We also found that the recruitment of HOIP to CD40 was TRAF2-dependent and that overexpression of a truncated HOIP mutant partially inhibited CD40-mediated NF-κB activation.
T39 566-654 Sentence denotes These results support the hypothesis that HOIP plays a role in CD40 signal transduction.
T40 655-839 Sentence denotes To further define the role and evaluate the importance of HOIP in CD40 signaling, we used somatic cell gene targeting to disrupt the gene encoding HOIP in the mouse B cell line A20.2J.
T41 840-1042 Sentence denotes This cell line has been particularly useful in the characterization of CD40 signaling mechanisms due to the relative ease with which its genome can be modified by homologous recombination [7], [8], [9].
T42 1043-1216 Sentence denotes We used a targeting vector capable of undergoing homologous recombination with Rnf31 (the gene encoding HOIP) to disrupt the coding sequence of the gene in exon 5 (Fig. 1A).
T43 1217-1400 Sentence denotes Following introduction of the vector, the neomycin-resistant clones that arose were screened by PCR amplification of genomic DNA to identify cells containing a disrupted Rnf31 allele.
T44 1401-1576 Sentence denotes To remove the selectable marker gene cassette from the disrupted Rnf31 allele, recombinant cell lines were transiently transfected with a plasmid that encodes Cre recombinase.
T45 1577-1719 Sentence denotes This step allowed us to perform a second round of targeting and drug selection, generating cells in which both copies of Rnf31 were disrupted.
T46 1720-1787 Sentence denotes Two independent clonal cell lines were chosen for further analysis.
T47 1788-1971 Sentence denotes HOIP protein expression was undetectable in both cell lines, as determined by Western blot analysis of cell lysates (Fig. 1B), demonstrating that the targeting process was successful.
T48 1972-2071 Sentence denotes In the text that follows, the two gene-targeted cell lines are referred to as HOIP-deficient cells.
T49 2072-2136 Sentence denotes 10.1371/journal.pone.0023061.g001 Figure 1 HOIP gene targeting.
T50 2137-2236 Sentence denotes (A) Regions of Rnf31 gene sequence (bottom) used in the HOIP gene targeting vector (top) are shown.
T51 2237-2389 Sentence denotes Arrows (F and R) indicate approximate positions of sequences homologous to oligonucleotides used for PCR-mediated detection of homologous recombination.
T52 2390-2591 Sentence denotes Homologous recombination of the vector with Rnf31 resulted in a small chromosomal deletion and the in-frame insertion of neomycin phosphotransferase (NeoR) into the amino-terminal HOIP coding sequence.
T53 2592-2747 Sentence denotes A diphtheria toxin (DT) cassette in the vector facilitates the negative selection of cells in which random chromosomal insertion of the vector takes place.
T54 2748-2823 Sentence denotes LoxP sequences allow the Cre-mediated deletion of the NeoR coding sequence.
T55 2824-3039 Sentence denotes The SV40pA sequence helps to ensure disruption of gene expression after deletion of the NeoR sequence. (B) Anti-HOIP and anti-FLAG Western blots of cell lysates from A20.2J cells and HOIP-deficient (HOIP-/-) clones.
T56 3040-3158 Sentence denotes A partial decrease in HOIP protein expression is evident in cells following disruption of one copy of Rnf31 (HOIP−/+).
T57 3159-3301 Sentence denotes HOIP expression in clones reconstituted with an empty retroviral vector (pMIP) or a retroviral vector encoding FLAG-tagged HOIP is also shown.
T58 3302-3349 Sentence denotes Approximate molecular weight of HOIP is 120 kD.
T59 3352-3643 Sentence denotes To enable us to confirm that any signaling or functional defects observed in HOIP-deficient cells were due to disruption of the Rnf31 gene, both HOIP-deficient cell lines were transduced with a retrovirus encoding FLAG-tagged wild-type HOIP, thus restoring HOIP protein expression (Fig. 1B).
T60 3644-3700 Sentence denotes These cells are referred to as HOIP-reconstituted cells.
T61 3701-3902 Sentence denotes In the experiments that follow, responses by these cells were compared to those of A20.2J cells and HOIP-deficient cells transduced with a retroviral vector (pMIP) lacking a cDNA insert (empty vector).

ICD10

Id Subject Object Predicate Lexical cue
T15255 2594-2604 http://purl.bioontology.org/ontology/ICD10/A36.9 denotes diphtheria
T15256 2594-2604 http://purl.bioontology.org/ontology/ICD10/A36 denotes diphtheria

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T3782 14-18 Q96EP0 denotes HOIP
T3783 64-69 Q96EP0 denotes Rnf31
T3784 89-93 Q96EP0 denotes HOIP
T3785 138-142 Q96EP0 denotes HOIP
T3786 163-167 P25942 denotes CD40
T3787 306-310 P25942 denotes CD40
T3788 316-320 Q96EP0 denotes HOIP
T3789 430-434 Q96EP0 denotes HOIP
T3790 438-442 P25942 denotes CD40
T3791 447-452 Q12933 denotes TRAF2
T3792 502-506 Q96EP0 denotes HOIP
T3793 534-538 P25942 denotes CD40
T3794 608-612 Q96EP0 denotes HOIP
T3795 629-633 P25942 denotes CD40
T3796 713-717 Q96EP0 denotes HOIP
T3797 721-725 P25942 denotes CD40
T3798 802-806 Q96EP0 denotes HOIP
T3799 832-835 P21580 denotes A20
T3800 911-915 P25942 denotes CD40
T3801 948-956 Q04864 denotes relative
T3802 1122-1127 Q96EP0 denotes Rnf31
T3803 1147-1151 Q96EP0 denotes HOIP
T3804 1387-1392 Q96EP0 denotes Rnf31
T3805 1466-1471 Q96EP0 denotes Rnf31
T3806 1560-1563 P06956 denotes Cre
T3807 1698-1703 Q96EP0 denotes Rnf31
T3808 1788-1792 Q96EP0 denotes HOIP
T3809 2050-2054 Q96EP0 denotes HOIP
T3810 3429-3433 Q96EP0 denotes HOIP
T3811 3480-3485 Q96EP0 denotes Rnf31
T3812 3497-3501 Q96EP0 denotes HOIP
T3813 3588-3592 Q96EP0 denotes HOIP
T3814 3609-3613 Q96EP0 denotes HOIP
T3815 3675-3679 Q96EP0 denotes HOIP
T3816 3784-3787 P21580 denotes A20
T3817 3801-3805 Q96EP0 denotes HOIP
T15228 2116-2120 Q96EP0 denotes HOIP
T15229 2152-2157 Q96EP0 denotes Rnf31
T15230 2193-2197 Q96EP0 denotes HOIP
T15231 2434-2439 Q96EP0 denotes Rnf31
T15232 2570-2574 Q96EP0 denotes HOIP
T15233 2773-2776 P06956 denotes Cre
T15234 2936-2940 Q96EP0 denotes HOIP
T15235 2990-2993 P21580 denotes A20
T15236 3007-3011 Q96EP0 denotes HOIP
T15237 3023-3027 Q96EP0 denotes HOIP
T15238 3062-3066 Q96EP0 denotes HOIP
T15239 3142-3147 Q96EP0 denotes Rnf31
T15240 3149-3153 Q96EP0 denotes HOIP
T15241 3159-3163 Q96EP0 denotes HOIP
T15242 3282-3286 Q96EP0 denotes HOIP
T15243 3334-3338 Q96EP0 denotes HOIP

test2

Id Subject Object Predicate Lexical cue Negation Speculation
T2762 14-18 Protein denotes HOIP
T2763 19-28 Negative_regulation denotes deficient
T2764 50-60 Negative_regulation denotes disruption
T2765 64-69 Protein denotes Rnf31
T2766 89-93 Protein denotes HOIP
T2767 138-142 Protein denotes HOIP
T2768 146-155 Binding denotes recruited
T2769 291-302 Binding denotes interaction
T2770 306-310 Protein denotes CD40
T2771 316-320 Protein denotes HOIP
T2772 415-426 Binding denotes recruitment true true
T2773 430-434 Protein denotes HOIP
T2774 438-442 Protein denotes CD40
T2775 447-452 Protein denotes TRAF2
T2776 453-462 Regulation denotes dependent
T2777 502-506 Protein denotes HOIP
T2778 534-538 Protein denotes CD40
T2779 608-612 Protein denotes HOIP
T2780 629-633 Protein denotes CD40
T2781 713-717 Protein denotes HOIP
T2782 721-725 Protein denotes CD40
T2783 776-783 Negative_regulation denotes disrupt
T2784 802-806 Protein denotes HOIP
T2785 911-915 Protein denotes CD40
T2786 1122-1127 Protein denotes Rnf31
T2787 1147-1151 Protein denotes HOIP
T2788 1377-1386 Negative_regulation denotes disrupted
T2789 1387-1392 Protein denotes Rnf31
T2790 1456-1465 Negative_regulation denotes disrupted
T2791 1466-1471 Protein denotes Rnf31
T2792 1560-1575 Protein denotes Cre recombinase
T2793 1698-1703 Protein denotes Rnf31
T2794 1709-1718 Negative_regulation denotes disrupted
T2795 1788-1792 Protein denotes HOIP
T2796 1801-1811 Gene_expression denotes expression
T2797 2050-2054 Protein denotes HOIP
T2798 2055-2064 Negative_regulation denotes deficient
T2799 3429-3433 Protein denotes HOIP
T2800 3434-3443 Negative_regulation denotes deficient
T2801 3462-3472 Negative_regulation denotes disruption
T2802 3480-3485 Protein denotes Rnf31
T2803 3497-3501 Protein denotes HOIP
T2804 3502-3511 Negative_regulation denotes deficient
T2805 3588-3592 Protein denotes HOIP
T2806 3599-3608 Negative_regulation denotes restoring
T2807 3609-3613 Protein denotes HOIP
T2808 3622-3632 Gene_expression denotes expression
T2809 3675-3679 Protein denotes HOIP
T2810 3801-3805 Protein denotes HOIP
T2811 3806-3815 Negative_regulation denotes deficient
T14759 2116-2120 Protein denotes HOIP
T14760 2152-2157 Protein denotes Rnf31
T14761 2193-2197 Protein denotes HOIP
T14762 2434-2439 Protein denotes Rnf31
T14763 2440-2448 Positive_regulation denotes resulted
T14764 2570-2574 Protein denotes HOIP
T14765 3007-3011 Protein denotes HOIP
T14766 3012-3021 Negative_regulation denotes deficient
T14767 3023-3027 Protein denotes HOIP
T14768 3050-3058 Negative_regulation denotes decrease
T14769 3062-3066 Protein denotes HOIP
T14770 3075-3085 Gene_expression denotes expression
T14771 3116-3126 Negative_regulation denotes disruption
T14772 3142-3147 Protein denotes Rnf31
T14773 3149-3153 Protein denotes HOIP
T14774 3159-3163 Protein denotes HOIP
T14775 3164-3174 Gene_expression denotes expression
T14776 3282-3286 Protein denotes HOIP
T14777 3334-3338 Protein denotes HOIP
R2078 T2762 T2763 themeOf HOIP,deficient
R2079 T2765 T2764 themeOf Rnf31,disruption
R2080 T2766 T2765 equivalentTo HOIP,Rnf31
R2081 T2767 T2768 themeOf HOIP,recruited
R2082 T2770 T2769 themeOf CD40,interaction
R2083 T2771 T2769 themeOf HOIP,interaction
R2084 T2772 T2776 themeOf recruitment,dependent
R2085 T2773 T2772 themeOf HOIP,recruitment
R2086 T2774 T2772 themeOf CD40,recruitment
R2087 T2775 T2776 causeOf TRAF2,dependent
R2088 T2784 T2783 themeOf HOIP,disrupt
R2089 T2787 T2786 equivalentTo HOIP,Rnf31
R2090 T2789 T2788 themeOf Rnf31,disrupted
R2091 T2791 T2790 themeOf Rnf31,disrupted
R2092 T2793 T2794 themeOf Rnf31,disrupted
R2093 T2795 T2796 themeOf HOIP,expression
R2094 T2797 T2798 themeOf HOIP,deficient
R2095 T2799 T2800 themeOf HOIP,deficient
R2096 T2802 T2801 themeOf Rnf31,disruption
R2097 T2803 T2804 themeOf HOIP,deficient
R2098 T2807 T2808 themeOf HOIP,expression
R2099 T2808 T2806 themeOf expression,restoring
R2100 T2810 T2811 themeOf HOIP,deficient
R11690 T14765 T14766 themeOf HOIP,deficient
R11691 T14769 T14770 themeOf HOIP,expression
R11692 T14770 T14768 themeOf expression,decrease
R11693 T14771 T14768 causeOf disruption,decrease
R11694 T14773 T14772 equivalentTo HOIP,Rnf31
R11695 T14774 T14775 themeOf HOIP,expression