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PMC:3148254 / 4902-22084 JSONTXT

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bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue Negation Speculation
T2812 23-27 Protein denotes HOIP
T2813 28-37 Negative_regulation denotes deficient
T2814 59-69 Negative_regulation denotes disruption
T2815 73-78 Protein denotes Rnf31
T2816 98-102 Protein denotes HOIP
T2817 147-151 Protein denotes HOIP
T2818 155-164 Binding denotes recruited
T2819 300-311 Binding denotes interaction
T2820 315-319 Protein denotes CD40
T2821 325-329 Protein denotes HOIP
T2822 424-435 Binding denotes recruitment
T2823 439-443 Protein denotes HOIP
T2824 447-451 Protein denotes CD40
T2825 456-461 Protein denotes TRAF2
T2826 462-471 Regulation denotes dependent
T2827 511-515 Protein denotes HOIP
T2828 543-547 Protein denotes CD40
T2829 617-621 Protein denotes HOIP
T2830 638-642 Protein denotes CD40
T2831 722-726 Protein denotes HOIP
T2832 730-734 Protein denotes CD40
T2833 785-792 Negative_regulation denotes disrupt
T2834 811-815 Protein denotes HOIP
T2835 920-924 Protein denotes CD40
T2836 1131-1136 Protein denotes Rnf31
T2837 1156-1160 Protein denotes HOIP
T2838 1386-1395 Negative_regulation denotes disrupted
T2839 1396-1401 Protein denotes Rnf31
T2840 1465-1474 Negative_regulation denotes disrupted
T2841 1475-1480 Protein denotes Rnf31
T2842 1569-1584 Protein denotes Cre recombinase
T2843 1707-1712 Protein denotes Rnf31
T2844 1718-1727 Negative_regulation denotes disrupted
T2845 1797-1801 Protein denotes HOIP
T2846 1810-1820 Gene_expression denotes expression true true
T2847 2059-2063 Protein denotes HOIP
T2848 2064-2073 Negative_regulation denotes deficient
T2849 3438-3442 Protein denotes HOIP
T2850 3443-3452 Negative_regulation denotes deficient
T2851 3471-3481 Negative_regulation denotes disruption
T2852 3489-3494 Protein denotes Rnf31
T2853 3506-3510 Protein denotes HOIP
T2854 3511-3520 Negative_regulation denotes deficient
T2855 3597-3601 Protein denotes HOIP
T2856 3608-3617 Positive_regulation denotes restoring
T2857 3618-3622 Protein denotes HOIP
T2858 3631-3641 Gene_expression denotes expression
T2859 3684-3688 Protein denotes HOIP
T2860 3689-3702 Positive_regulation denotes reconstituted
T2861 3810-3814 Protein denotes HOIP
T2862 3815-3824 Negative_regulation denotes deficient
T4174 3913-3917 Protein denotes HOIP
T4175 3921-3929 Positive_regulation denotes required
T4176 3921-3929 Positive_regulation denotes required
T4177 3934-3938 Protein denotes CD40
T4178 3939-3947 Positive_regulation denotes mediated
T4179 3939-3947 Positive_regulation denotes mediated
T4180 3948-3952 Protein denotes CD80
T4181 3953-3965 Positive_regulation denotes upregulation
T4182 3970-3980 Positive_regulation denotes activation
T4183 3984-4000 Protein denotes germline epsilon
T4184 4001-4014 Transcription denotes transcription
T4185 4015-4025 Binding denotes Engagement
T4186 4029-4033 Protein denotes CD40
T4187 4045-4056 Positive_regulation denotes upregulates
T4188 4057-4067 Gene_expression denotes expression
T4189 4071-4075 Protein denotes CD80
T4190 4228-4232 Protein denotes HOIP
T4191 4236-4240 Protein denotes CD40
T4192 4267-4279 Positive_regulation denotes upregulation
T4193 4283-4287 Protein denotes CD80
T4194 4288-4295 Positive_regulation denotes induced true
T4195 4299-4309 Binding denotes engagement
T4196 4313-4317 Protein denotes CD40
T4197 4338-4342 Protein denotes HOIP
T4198 4358-4362 Protein denotes HOIP
T4199 4363-4376 Positive_regulation denotes reconstituted
T4200 4433-4437 Protein denotes CD40
T4201 4438-4448 Positive_regulation denotes stimulated
T4202 4449-4461 Positive_regulation denotes upregulation
T4203 4465-4469 Protein denotes CD80
T4204 4487-4494 Negative_regulation denotes reduced
T4205 4502-4506 Protein denotes HOIP
T4206 4507-4516 Negative_regulation denotes deficient
T4207 4544-4556 Positive_regulation denotes upregulation
T4208 4560-4564 Protein denotes CD80
T4209 4581-4585 Protein denotes HOIP
T4210 4586-4599 Positive_regulation denotes reconstituted
T4211 4627-4633 Negative_regulation denotes defect
T4212 4637-4641 Protein denotes CD80
T4213 4642-4654 Positive_regulation denotes upregulation
T4214 4662-4666 Protein denotes HOIP
T4215 4667-4676 Negative_regulation denotes deficient
T4216 4687-4690 Positive_regulation denotes due
T4217 4698-4708 Negative_regulation denotes disruption
T4218 4712-4717 Protein denotes Rnf31
T4219 5337-5341 Protein denotes CD40
T4220 5762-5766 Protein denotes IL-4
T4221 5771-5775 Protein denotes CD40
T4222 5977-5981 Protein denotes HOIP
T4223 6094-6098 Protein denotes CD40
T4224 6118-6122 Protein denotes IL-4
T4225 6138-6148 Transcription denotes production true
T4226 6152-6168 Protein denotes germline epsilon
T4227 6170-6173 Protein denotes GLε
T4228 6251-6255 Protein denotes IL-4
T4229 6256-6263 Positive_regulation denotes induced
T4230 6264-6267 Protein denotes GLε
T4231 6268-6281 Transcription denotes transcription
T4232 6352-6355 Protein denotes GLε
T4233 6356-6366 Gene_expression denotes expression
T4234 6384-6388 Protein denotes HOIP
T4235 6389-6398 Negative_regulation denotes deficient
T4236 6470-6474 Protein denotes IL-4
T4237 6493-6496 Protein denotes GLε
T4238 6497-6507 Gene_expression denotes expression
T4239 6513-6520 Positive_regulation denotes induced
T4240 6524-6528 Protein denotes HOIP
T4241 6529-6542 Positive_regulation denotes reconstituted
T4242 6607-6611 Protein denotes IL-4
T4243 6655-6659 Protein denotes HOIP
T4244 6705-6709 Protein denotes CD40
T5461 7584-7588 Protein denotes HOIP
T5462 7598-7602 Protein denotes CD40
T5463 7677-7681 Protein denotes HOIP
T5464 7709-7713 Protein denotes CD40
T5465 7770-7774 Protein denotes HOIP
T5466 7803-7807 Protein denotes CD40
T5467 7895-7900 Protein denotes CD154
T5468 7902-7913 Protein denotes CD40 ligand
T5469 7915-7924 Gene_expression denotes expressed
T5470 8066-8070 Protein denotes CD40
T5471 8081-8091 Binding denotes associated
T5472 8092-8097 Protein denotes CD154
T5473 8432-8443 Regulation denotes responsible
T5474 8448-8460 Negative_regulation denotes sequestering
T5475 8461-8475 Protein denotes NF-κB subunits
T5476 8652-8656 Protein denotes CD40
T5477 8657-8665 Positive_regulation denotes mediated
T5478 8657-8665 Positive_regulation denotes mediated
T5479 8666-8681 Phosphorylation denotes phosphorylation
T5480 8686-8697 Protein_catabolism denotes degradation
T5481 8701-8705 Protein denotes IκBα
T5482 8709-8713 Protein denotes HOIP
T5483 8714-8723 Negative_regulation denotes deficient
T5484 8747-8755 Negative_regulation denotes impaired
T5485 8747-8755 Negative_regulation denotes impaired
T5486 8902-8906 Protein denotes CD40
T5487 8907-8917 Binding denotes engagement
T5488 8928-8932 Protein denotes CD40
T5489 8960-8964 Protein denotes HOIP
T5490 8965-8974 Negative_regulation denotes deficient
T5491 9054-9058 Protein denotes CD40
T5492 9098-9102 Protein denotes HOIP
T5493 9103-9116 Positive_regulation denotes reconstituted
T5494 9226-9230 Protein denotes HOIP
T5495 9231-9241 Gene_expression denotes expression true
T6577 10368-10372 Protein denotes CD40
T6578 10395-10399 Protein denotes CD40
T6579 10452-10456 Protein denotes HOIP
T6580 10457-10466 Negative_regulation denotes deficient
T6581 10488-10492 Protein denotes HOIP
T6582 10619-10623 Protein denotes HOIP
T6583 10627-10636 Binding denotes recruited
T6584 10672-10677 Protein denotes TRAF2
T6585 10678-10687 Regulation denotes dependent
T6586 10712-10716 Protein denotes HOIP
T6587 10717-10737 Regulation denotes functions downstream true
T6588 10741-10746 Protein denotes TRAF2
T6589 10787-10792 Protein denotes TRAF2
T6590 10793-10803 Binding denotes associated
T6591 10793-10803 Binding denotes associated
T6592 10813-10818 Protein denotes cIAP1
T6593 10823-10828 Protein denotes cIAP2
T6594 10836-10840 Regulation denotes role
T6595 10836-10840 Regulation denotes role
T6596 10836-10840 Regulation denotes role
T6597 10836-10840 Regulation denotes role
T6598 10848-10859 Binding denotes recruitment
T6599 10848-10859 Binding denotes recruitment
T6600 10863-10867 Protein denotes HOIP
T6601 10871-10876 Protein denotes TNFR1
T6602 10881-10885 Protein denotes CD40
T6603 10929-10940 Binding denotes association
T6604 10944-10948 Protein denotes HOIP
T6605 10954-10958 Protein denotes CD40
T6606 10979-10986 Regulation denotes altered true
T6607 11193-11200 Negative_regulation denotes reduced
T6608 11215-11220 Protein denotes cIAP1
T6609 11221-11231 Binding denotes associated
T6610 11360-11368 Positive_regulation denotes resulted
T6611 11360-11368 Positive_regulation denotes resulted
T6612 11398-11406 Negative_regulation denotes decrease
T6613 11434-11438 Protein denotes HOIP
T6614 11444-11453 Binding denotes recovered
T6615 11457-11461 Protein denotes CD40
T6616 11506-11514 Positive_regulation denotes increase
T6617 11550-11560 Binding denotes recognized
T6618 11637-11642 Regulation denotes alter true
T6619 11677-11681 Protein denotes HOIP
T6620 11767-11773 Regulation denotes alters
T6621 11797-11801 Protein denotes CD40
T6622 11802-11812 Binding denotes associated
T6623 11813-11817 Protein denotes HOIP
T6624 11922-11926 Protein denotes cIAP
T6625 11936-11945 Regulation denotes influence
T6626 11936-11945 Regulation denotes influence
T6627 11950-11961 Binding denotes recruitment
T6628 11966-11997 Protein_modification denotes post-translational modification
T6629 12007-12011 Protein denotes CD40
T6630 12012-12022 Binding denotes associated
T6631 12023-12027 Protein denotes HOIP
T6632 14511-14515 Protein denotes HOIP
T6633 14519-14530 Regulation denotes responsible true
T6634 14539-14550 Binding denotes recruitment
T6635 14591-14595 Protein denotes CD40
T6636 14649-14653 Protein denotes HOIP
T6637 14654-14663 Negative_regulation denotes deficient
T6638 14668-14672 Protein denotes HOIP
T6639 14673-14686 Positive_regulation denotes reconstituted
T6640 14801-14805 Protein denotes HOIP
T6641 14817-14822 Protein denotes TRAF2
T6642 14824-14829 Protein denotes TRAF3
T6643 14831-14835 Protein denotes IKKα
T6644 14836-14837 Protein denotes β
T6645 14843-14847 Protein denotes IKKγ
T6646 14868-14873 Protein denotes TRAF2
T6647 14875-14880 Protein denotes TRAF3
T6648 14886-14891 Protein denotes cIAP1
T6649 14895-14899 Protein denotes CD40
T6650 14924-14928 Protein denotes HOIP
T6651 15011-15015 Protein denotes HOIP
T6652 15023-15031 Positive_regulation denotes required true
T6653 15023-15031 Positive_regulation denotes required true
T6654 15023-15031 Positive_regulation denotes required true
T6655 15040-15051 Binding denotes association
T6656 15040-15051 Binding denotes association
T6657 15040-15051 Binding denotes association
T6658 15075-15079 Protein denotes CD40
T6659 15094-15098 Protein denotes IKKα
T6660 15099-15100 Protein denotes β
T6661 15105-15109 Protein denotes IKKγ
T6662 15133-15137 Protein denotes CD40
T6663 15172-15176 Protein denotes HOIP
T6664 15177-15186 Negative_regulation denotes deficient
T6665 15221-15225 Protein denotes IKKα
T6666 15226-15227 Protein denotes β
T6667 15232-15236 Protein denotes IKKγ
T6668 15240-15244 Protein denotes CD40
T6669 15269-15273 Protein denotes HOIP
T6670 15274-15287 Positive_regulation denotes reconstituted
T6671 15457-15464 Negative_regulation denotes absence
T6672 15468-15472 Protein denotes HOIP
T6673 15473-15483 Gene_expression denotes expression
T6674 15510-15514 Protein denotes HOIP
T6675 15651-15655 Protein denotes IKKγ
T6676 15667-15671 Protein denotes CD40
T6677 15830-15834 Protein denotes IKKγ
T6678 15964-15968 Protein denotes IKKγ
T6679 15987-15991 Protein denotes CD40
T6680 16229-16233 Protein denotes IKKγ
T6681 16298-16308 Gene_expression denotes expressing
T6682 16324-16328 Protein denotes IKKγ
T6683 16344-16355 Binding denotes recruitment
T6684 16359-16363 Protein denotes IKKγ
T6685 16367-16371 Protein denotes CD40
T6686 16372-16380 Positive_regulation denotes requires
T6687 16381-16385 Protein denotes HOIP
T6688 16459-16463 Protein denotes IKKγ
T6689 16467-16471 Protein denotes CD40
T6690 16673-16677 Protein denotes CD40
T6691 16678-16688 Binding denotes associated
T6692 16689-16693 Protein denotes IKKγ
T6693 16906-16910 Protein denotes CD40
T6694 16911-16921 Binding denotes associated
T6695 16922-16926 Protein denotes IKKγ
T6696 16988-17003 Phosphorylation denotes phosphorylation
T6697 17042-17046 Protein denotes HOIP
T6698 17050-17058 Positive_regulation denotes required
T6699 17067-17078 Binding denotes association
T6700 17082-17086 Protein denotes IKKγ
T6701 17092-17096 Protein denotes CD40
T6702 17129-17136 Positive_regulation denotes coupled
T6703 17140-17172 Protein_modification denotes post-translational modifications
T6704 17176-17180 Protein denotes IKKγ
T14778 2125-2129 Protein denotes HOIP
T14779 2161-2166 Protein denotes Rnf31
T14780 2202-2206 Protein denotes HOIP
T14781 2443-2448 Protein denotes Rnf31
T14782 2579-2583 Protein denotes HOIP
T14783 3016-3020 Protein denotes HOIP
T14784 3021-3030 Negative_regulation denotes deficient
T14785 3032-3036 Protein denotes HOIP
T14786 3059-3067 Negative_regulation denotes decrease
T14787 3071-3075 Protein denotes HOIP
T14788 3084-3094 Gene_expression denotes expression
T14789 3115-3124 Positive_regulation denotes following
T14790 3125-3135 Negative_regulation denotes disruption
T14791 3151-3156 Protein denotes Rnf31
T14792 3158-3162 Protein denotes HOIP
T14793 3168-3172 Protein denotes HOIP
T14794 3173-3183 Gene_expression denotes expression
T14795 3291-3295 Protein denotes HOIP
T14796 3343-3347 Protein denotes HOIP
T15371 4763-4767 Protein denotes CD40
T15372 4768-4776 Positive_regulation denotes mediated
T15373 4777-4781 Protein denotes CD80
T15374 4782-4794 Positive_regulation denotes upregulation
T15375 4798-4807 Negative_regulation denotes defective
T15376 4811-4815 Protein denotes HOIP
T15377 4816-4825 Negative_regulation denotes deficient
T15378 4956-4966 Gene_expression denotes expression true
T15379 4970-4974 Protein denotes CD80
T15380 5118-5122 Protein denotes HOIP
T15381 5123-5132 Negative_regulation denotes deficient
T15382 5134-5138 Protein denotes HOIP
T15383 5182-5186 Protein denotes HOIP
T15384 5187-5195 Negative_regulation denotes reversed
T15385 5196-5205 Negative_regulation denotes defective
T15386 5206-5210 Protein denotes CD80
T15387 5211-5223 Positive_regulation denotes upregulation
T15715 6765-6768 Protein denotes GLε
T15716 6769-6782 Transcription denotes transcription
T15717 6786-6795 Negative_regulation denotes defective
T15718 6799-6803 Protein denotes CD40
T15719 6815-6819 Protein denotes HOIP
T15720 6820-6829 Negative_regulation denotes deficient
T15721 6848-6852 Protein denotes HOIP
T15722 6853-6862 Negative_regulation denotes deficient
T15723 6931-6935 Protein denotes HOIP
T15724 6965-6969 Protein denotes HOIP
T15725 6970-6979 Negative_regulation denotes deficient
T15726 7004-7008 Protein denotes HOIP
T15727 7030-7034 Protein denotes HOIP
T15728 7040-7044 Protein denotes HOIP
T15729 7149-7153 Protein denotes IL-4
T15730 7271-7274 Protein denotes GLε
T15731 7275-7286 Transcription denotes transcripts true
T15732 7329-7334 Protein denotes Hprt1
T15733 7335-7346 Transcription denotes transcripts
T15734 7561-7565 Protein denotes HOIP
T15735 7566-7575 Negative_regulation denotes deficient
T16768 12135-12139 Protein denotes HOIP
T16769 12140-12149 Negative_regulation denotes deficient
T16770 12184-12191 Negative_regulation denotes reduces
T16771 12192-12203 Binding denotes recruitment
T16772 12207-12212 Protein denotes cIAP1
T16773 12216-12220 Protein denotes CD40
T16774 12229-12235 Regulation denotes modify true
T16775 12236-12240 Protein denotes HOIP
T16776 12241-12252 Binding denotes recruitment
T16777 12847-12852 Protein denotes cIAP1
T16778 12854-12859 Protein denotes TRAF2
T16779 12861-12866 Protein denotes TRAF3
T16780 12872-12876 Protein denotes HOIP
T16781 12993-12997 Protein denotes CD40
T16782 13068-13072 Protein denotes HOIP
T16783 13073-13082 Negative_regulation denotes deficient
T16784 13171-13175 Protein denotes HOIP
T16785 13307-13311 Protein denotes CD40
T16786 13313-13318 Protein denotes TRAF2
T16787 13320-13325 Protein denotes TRAF3
T16788 13327-13332 Protein denotes cIAP1
T16789 13334-13338 Protein denotes HOIP
T16790 13340-13344 Protein denotes IKKα
T16791 13345-13346 Protein denotes β
T16792 13352-13356 Protein denotes IKKγ
T16793 13373-13377 Protein denotes HOIP
T16794 13378-13388 Gene_expression denotes expression
T16798 13652-13656 Protein denotes HOIP
T16799 13657-13666 Regulation denotes dependent true
T16800 13667-13678 Binding denotes recruitment
T16801 13682-13686 Protein denotes IKKγ
T16802 13690-13694 Protein denotes CD40
T16803 13712-13716 Protein denotes HOIP
T16804 13717-13726 Negative_regulation denotes deficient
T16805 13728-13732 Protein denotes HOIP
T16806 13851-13855 Protein denotes IKKγ
T16807 13880-13884 Protein denotes pMIP
T16808 13889-13893 Protein denotes IKKγ
T16809 14095-14099 Protein denotes IKKγ
T16810 14118-14123 Protein denotes TRAF2
T16811 14383-14388 Protein denotes TRAF3
T16812 14393-14397 Protein denotes HOIP
T16162 9287-9291 Protein denotes CD40
T16163 9344-9348 Protein denotes HOIP
T16164 9349-9358 Negative_regulation denotes deficient
T16165 9366-9370 Protein denotes CD40
T16166 9407-9411 Protein denotes HOIP
T16167 9412-9421 Negative_regulation denotes deficient
T16168 9541-9545 Protein denotes HOIP
T16169 9546-9555 Negative_regulation denotes deficient
T16170 9607-9611 Protein denotes HOIP
T16171 9613-9617 Protein denotes HOIP
T16172 9623-9627 Protein denotes HOIP
T16173 9656-9661 Protein denotes CD154
T16174 9662-9672 Gene_expression denotes expressing
T16175 9774-9781 Negative_regulation denotes lacking
T16176 9782-9787 Protein denotes CD154
T16177 9895-9907 Protein denotes Phospho-IκBα
T16178 9909-9914 Protein denotes pIκBα
T16179 9982-9986 Protein denotes IκBα
T16180 10069-10073 Protein denotes IκBα
T16181 10287-10291 Protein denotes HOIP
T16182 10292-10301 Negative_regulation denotes deficient
T16795 13443-13447 Protein denotes CD40
T16796 13606-13610 Protein denotes HOIP
T16797 13611-13620 Negative_regulation denotes deficient
R2101 T2812 T2813 themeOf HOIP,deficient
R2102 T2815 T2814 themeOf Rnf31,disruption
R2103 T2816 T2815 equivalentTo HOIP,Rnf31
R2104 T2817 T2818 themeOf HOIP,recruited
R2105 T2820 T2819 themeOf CD40,interaction
R2106 T2821 T2819 themeOf HOIP,interaction
R2107 T2822 T2826 themeOf recruitment,dependent
R2108 T2823 T2822 themeOf HOIP,recruitment
R2109 T2824 T2822 themeOf CD40,recruitment
R2110 T2825 T2826 causeOf TRAF2,dependent
R2111 T2834 T2833 themeOf HOIP,disrupt
R2112 T2837 T2836 equivalentTo HOIP,Rnf31
R2113 T2839 T2838 themeOf Rnf31,disrupted
R2114 T2841 T2840 themeOf Rnf31,disrupted
R2115 T2843 T2844 themeOf Rnf31,disrupted
R2116 T2845 T2846 themeOf HOIP,expression
R2117 T2847 T2848 themeOf HOIP,deficient
R2118 T2849 T2850 themeOf HOIP,deficient
R2119 T2852 T2851 themeOf Rnf31,disruption
R2120 T2853 T2854 themeOf HOIP,deficient
R2121 T2857 T2858 themeOf HOIP,expression
R2122 T2858 T2856 themeOf expression,restoring
R2123 T2859 T2860 themeOf HOIP,reconstituted
R2124 T2861 T2862 themeOf HOIP,deficient
R3207 T4174 T4176 causeOf HOIP,required
R3208 T4174 T4175 causeOf HOIP,required
R3209 T4178 T4175 themeOf mediated,required
R3210 T4179 T4176 themeOf mediated,required
R3211 T4180 T4181 themeOf CD80,upregulation
R3212 T4181 T4179 themeOf upregulation,mediated
R3213 T4182 T4178 themeOf activation,mediated
R3214 T4183 T4184 themeOf germline epsilon,transcription
R3215 T4184 T4182 themeOf transcription,activation
R3216 T4185 T4187 causeOf Engagement,upregulates
R3217 T4186 T4185 themeOf CD40,Engagement
R3218 T4188 T4187 themeOf expression,upregulates
R3219 T4189 T4188 themeOf CD80,expression
R3220 T4192 T4194 themeOf upregulation,induced
R3221 T4193 T4192 themeOf CD80,upregulation
R3222 T4195 T4194 causeOf engagement,induced
R3223 T4196 T4195 themeOf CD40,engagement
R3224 T4198 T4199 themeOf HOIP,reconstituted
R3225 T4200 T4201 causeOf CD40,stimulated
R3226 T4202 T4201 themeOf upregulation,stimulated
R3227 T4202 T4204 themeOf upregulation,reduced
R3228 T4203 T4202 themeOf CD80,upregulation
R3229 T4205 T4206 themeOf HOIP,deficient
R3230 T4206 T4204 causeOf deficient,reduced
R3231 T4208 T4207 themeOf CD80,upregulation
R3232 T4209 T4210 themeOf HOIP,reconstituted
R3233 T4211 T4216 themeOf defect,due
R3234 T4212 T4213 themeOf CD80,upregulation
R3235 T4213 T4211 themeOf upregulation,defect
R3236 T4214 T4215 themeOf HOIP,deficient
R3237 T4215 T4211 causeOf deficient,defect
R3238 T4217 T4216 causeOf disruption,due
R3239 T4218 T4217 themeOf Rnf31,disruption
R3240 T4226 T4225 themeOf germline epsilon,production
R3241 T4227 T4226 equivalentTo GLε,germline epsilon
R3242 T4230 T4231 themeOf GLε,transcription
R3243 T4231 T4229 themeOf transcription,induced
R3244 T4232 T4233 themeOf GLε,expression
R3245 T4234 T4235 themeOf HOIP,deficient
R3246 T4237 T4238 themeOf GLε,expression
R3247 T4238 T4239 themeOf expression,induced
R3248 T4240 T4241 themeOf HOIP,reconstituted
R4184 T5467 T5469 themeOf CD154,expressed
R4185 T5468 T5467 equivalentTo CD40 ligand,CD154
R4186 T5472 T5471 themeOf CD154,associated
R4187 T5474 T5473 themeOf sequestering,responsible
R4188 T5475 T5474 themeOf NF-κB subunits,sequestering
R4189 T5476 T5477 causeOf CD40,mediated
R4190 T5476 T5478 causeOf CD40,mediated
R4191 T5477 T5484 themeOf mediated,impaired
R4192 T5478 T5485 themeOf mediated,impaired
R4193 T5479 T5478 themeOf phosphorylation,mediated
R4194 T5480 T5477 themeOf degradation,mediated
R4195 T5481 T5480 themeOf IκBα,degradation
R4196 T5481 T5479 themeOf IκBα,phosphorylation
R4197 T5482 T5483 themeOf HOIP,deficient
R4198 T5486 T5487 themeOf CD40,engagement
R4199 T5489 T5490 themeOf HOIP,deficient
R4200 T5492 T5493 themeOf HOIP,reconstituted
R4201 T5494 T5495 themeOf HOIP,expression
R5004 T6579 T6580 themeOf HOIP,deficient
R5005 T6582 T6583 themeOf HOIP,recruited
R5006 T6583 T6585 themeOf recruited,dependent
R5007 T6584 T6585 causeOf TRAF2,dependent
R5008 T6586 T6587 themeOf HOIP,functions downstream
R5009 T6588 T6587 causeOf TRAF2,functions downstream
R5010 T6589 T6590 themeOf TRAF2,associated
R5011 T6589 T6591 themeOf TRAF2,associated
R5012 T6592 T6590 themeOf cIAP1,associated
R5013 T6592 T6597 causeOf cIAP1,role
R5014 T6592 T6596 causeOf cIAP1,role
R5015 T6593 T6591 themeOf cIAP2,associated
R5016 T6593 T6594 causeOf cIAP2,role
R5017 T6593 T6595 causeOf cIAP2,role
R5018 T6598 T6597 themeOf recruitment,role
R5019 T6598 T6594 themeOf recruitment,role
R5020 T6599 T6596 themeOf recruitment,role
R5021 T6599 T6595 themeOf recruitment,role
R5022 T6600 T6598 themeOf HOIP,recruitment
R5023 T6600 T6599 themeOf HOIP,recruitment
R5024 T6601 T6598 themeOf TNFR1,recruitment
R5025 T6602 T6599 themeOf CD40,recruitment
R5026 T6603 T6606 themeOf association,altered
R5027 T6604 T6603 themeOf HOIP,association
R5028 T6605 T6603 themeOf CD40,association
R5029 T6608 T6609 themeOf cIAP1,associated
R5030 T6609 T6607 themeOf associated,reduced
R5031 T6612 T6611 themeOf decrease,resulted
R5032 T6613 T6612 themeOf HOIP,decrease
R5033 T6613 T6616 themeOf HOIP,increase
R5034 T6613 T6614 themeOf HOIP,recovered
R5035 T6613 T6617 themeOf HOIP,recognized
R5036 T6615 T6614 themeOf CD40,recovered
R5037 T6616 T6610 themeOf increase,resulted
R5038 T6619 T6618 themeOf HOIP,alter
R5039 T6621 T6622 themeOf CD40,associated
R5040 T6623 T6620 themeOf HOIP,alters
R5041 T6623 T6622 themeOf HOIP,associated
R5042 T6624 T6626 causeOf cIAP,influence
R5043 T6624 T6625 causeOf cIAP,influence
R5044 T6627 T6626 themeOf recruitment,influence
R5045 T6628 T6625 themeOf post-translational modification,influence
R5046 T6629 T6630 themeOf CD40,associated
R5047 T6631 T6627 themeOf HOIP,recruitment
R5048 T6631 T6628 themeOf HOIP,post-translational modification
R5049 T6631 T6630 themeOf HOIP,associated
R5050 T6632 T6633 causeOf HOIP,responsible
R5051 T6634 T6633 themeOf recruitment,responsible
R5052 T6635 T6634 themeOf CD40,recruitment
R5053 T6636 T6637 themeOf HOIP,deficient
R5054 T6638 T6639 themeOf HOIP,reconstituted
R5055 T6646 T6655 themeOf TRAF2,association
R5056 T6647 T6657 themeOf TRAF3,association
R5057 T6648 T6656 themeOf cIAP1,association
R5058 T6651 T6654 causeOf HOIP,required
R5059 T6651 T6653 causeOf HOIP,required
R5060 T6651 T6652 causeOf HOIP,required
R5061 T6655 T6654 themeOf association,required
R5062 T6656 T6652 themeOf association,required
R5063 T6657 T6653 themeOf association,required
R5064 T6658 T6655 themeOf CD40,association
R5065 T6658 T6657 themeOf CD40,association
R5066 T6658 T6656 themeOf CD40,association
R5067 T6663 T6664 themeOf HOIP,deficient
R5068 T6669 T6670 themeOf HOIP,reconstituted
R5069 T6672 T6673 themeOf HOIP,expression
R5070 T6673 T6671 themeOf expression,absence
R5071 T6682 T6681 themeOf IKKγ,expressing
R5072 T6683 T6686 themeOf recruitment,requires
R5073 T6684 T6683 themeOf IKKγ,recruitment
R5074 T6685 T6683 themeOf CD40,recruitment
R5075 T6687 T6686 causeOf HOIP,requires
R5076 T6690 T6691 themeOf CD40,associated
R5077 T6692 T6691 themeOf IKKγ,associated
R5078 T6693 T6694 themeOf CD40,associated
R5079 T6695 T6694 themeOf IKKγ,associated
R5080 T6695 T6696 themeOf IKKγ,phosphorylation
R5081 T6697 T6698 causeOf HOIP,required
R5082 T6699 T6698 themeOf association,required
R5083 T6699 T6702 causeOf association,coupled
R5084 T6700 T6699 themeOf IKKγ,association
R5085 T6701 T6699 themeOf CD40,association
R5086 T6703 T6702 themeOf post-translational modifications,coupled
R5087 T6704 T6703 themeOf IKKγ,post-translational modifications
R11699 T14788 T14786 themeOf expression,decrease
R11700 T14790 T14789 causeOf disruption,following
R11701 T14791 T14790 themeOf Rnf31,disruption
R11702 T14793 T14794 themeOf HOIP,expression
R12170 T15371 T15372 causeOf CD40,mediated
R12171 T15372 T15375 themeOf mediated,defective
R12172 T15373 T15374 themeOf CD80,upregulation
R12173 T15374 T15372 themeOf upregulation,mediated
R12174 T15376 T15377 themeOf HOIP,deficient
R12175 T15379 T15378 themeOf CD80,expression
R12176 T15380 T15381 themeOf HOIP,deficient
R12177 T15385 T15384 themeOf defective,reversed
R12178 T15386 T15387 themeOf CD80,upregulation
R12179 T15387 T15385 themeOf upregulation,defective
R12421 T15715 T15716 themeOf GLε,transcription
R12422 T15716 T15717 themeOf transcription,defective
R12423 T15719 T15720 themeOf HOIP,deficient
R12424 T15721 T15722 themeOf HOIP,deficient
R12425 T15724 T15725 themeOf HOIP,deficient
R12426 T15730 T15731 themeOf GLε,transcripts
R12427 T15732 T15733 themeOf Hprt1,transcripts
R12428 T15734 T15735 themeOf HOIP,deficient
R13169 T16768 T16769 themeOf HOIP,deficient
R13170 T16771 T16770 themeOf recruitment,reduces
R13171 T16772 T16771 themeOf cIAP1,recruitment
R13172 T16773 T16771 themeOf CD40,recruitment
R13173 T16775 T16776 themeOf HOIP,recruitment
R13174 T16776 T16774 themeOf recruitment,modify
R13175 T16782 T16783 themeOf HOIP,deficient
R13176 T16793 T16794 themeOf HOIP,expression
R13177 T16796 T16797 themeOf HOIP,deficient
R13178 T16798 T16799 causeOf HOIP,dependent
R13179 T16800 T16799 themeOf recruitment,dependent
R13180 T16801 T16800 themeOf IKKγ,recruitment
R13181 T16802 T16800 themeOf CD40,recruitment
R13182 T16803 T16804 themeOf HOIP,deficient
R11696 T14783 T14784 themeOf HOIP,deficient
R11697 T14786 T14789 themeOf decrease,following
R11698 T14787 T14788 themeOf HOIP,expression
R12750 T16163 T16164 themeOf HOIP,deficient
R12751 T16166 T16167 themeOf HOIP,deficient
R12752 T16168 T16169 themeOf HOIP,deficient
R12753 T16173 T16174 themeOf CD154,expressing
R12754 T16176 T16175 themeOf CD154,lacking
R12755 T16178 T16177 equivalentTo pIκBα,Phospho-IκBα
R12756 T16181 T16182 themeOf HOIP,deficient

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T3953 73-78 Protein denotes Rnf31
T3954 59-69 Transcription denotes disruption
T3955 172-176 Protein denotes CD40
T3956 315-319 Protein denotes CD40
T3957 300-311 Binding denotes interaction
T3958 439-443 Protein denotes HOIP
T3959 447-451 Protein denotes CD40
T3960 456-461 Protein denotes TRAF2
T3961 511-522 Protein denotes HOIP mutant
T3962 543-547 Protein denotes CD40
T3963 557-562 Protein denotes NF-κB
T3964 424-435 Binding denotes recruitment
T3965 481-495 Gene_expression denotes overexpression
T3966 481-495 Positive_regulation denotes overexpression
T3967 563-573 Positive_regulation denotes activation
T3968 638-642 Protein denotes CD40
T3969 730-734 Protein denotes CD40
T3970 920-924 Protein denotes CD40
T3971 1131-1136 Protein denotes Rnf31
T3972 1396-1401 Protein denotes Rnf31
T3973 1475-1480 Protein denotes Rnf31
T3974 1569-1584 Protein denotes Cre recombinase
T3975 1707-1712 Protein denotes Rnf31
T3976 1797-1809 Protein denotes HOIP protein
T3977 1810-1820 Gene_expression denotes expression
T3978 1825-1837 Gene_expression denotes undetectable
T3979 3489-3499 Protein denotes Rnf31 gene
T3980 3618-3630 Protein denotes HOIP protein
T3981 3471-3481 Negative_regulation denotes disruption
T3982 3631-3641 Gene_expression denotes expression
T5261 3934-3938 Protein denotes CD40
T5262 3948-3952 Protein denotes CD80
T5263 3984-4000 Protein denotes germline epsilon
T5264 3953-3965 Positive_regulation denotes upregulation
T5265 4001-4014 Transcription denotes transcription
T5266 3970-3980 Positive_regulation denotes activation
T5267 3921-3929 Positive_regulation denotes required
T5268 4029-4033 Protein denotes CD40
T5269 4071-4075 Protein denotes CD80
T5270 4015-4025 Binding denotes Engagement
T5271 4057-4067 Gene_expression denotes expression
T5272 4045-4056 Positive_regulation denotes upregulates
T5273 4236-4240 Protein denotes CD40
T5274 4283-4287 Protein denotes CD80
T5275 4313-4317 Protein denotes CD40
T5276 4267-4279 Positive_regulation denotes upregulation
T5277 4299-4309 Binding denotes engagement
T5278 4433-4437 Protein denotes CD40
T5279 4465-4469 Protein denotes CD80
T5280 4449-4461 Positive_regulation denotes upregulation
T5281 4487-4494 Negative_regulation denotes reduced
T5282 4560-4564 Protein denotes CD80
T5283 4637-4641 Protein denotes CD80
T5284 4712-4717 Protein denotes Rnf31
T5285 4544-4556 Positive_regulation denotes upregulation
T5286 4642-4654 Positive_regulation denotes upregulation
T5287 4698-4708 Negative_regulation denotes disruption
T5288 4627-4633 Negative_regulation denotes defect
T5289 5337-5341 Protein denotes CD40
T5290 5427-5441 Protein denotes immunoglobulin
T5291 5495-5509 Protein denotes immunoglobulin
T5292 5587-5590 Protein denotes IgM
T5293 5605-5608 Protein denotes IgA
T5294 5610-5613 Protein denotes IgG
T5295 5618-5621 Protein denotes IgE
T5296 5576-5586 Gene_expression denotes expressing
T5297 5595-5601 Binding denotes switch
T5298 5721-5724 Protein denotes IgE
T5299 5762-5766 Protein denotes IL-4
T5300 5771-5783 Protein denotes CD40 signals
T5301 5747-5758 Binding denotes combination
T5302 5732-5739 Positive_regulation denotes induced
T5303 5822-5825 Protein denotes IgE
T5304 5928-5931 Protein denotes IgE
T5305 5826-5836 Gene_expression denotes production
T5306 5808-5817 Positive_regulation denotes necessary
T5307 6094-6098 Protein denotes CD40
T5308 6118-6122 Protein denotes IL-4
T5309 6197-6215 Protein denotes Anti-CD40 antibody
T5310 6251-6255 Protein denotes IL-4
T5311 6216-6227 Positive_regulation denotes stimulation
T5312 6433-6437 Protein denotes CD40
T5313 6461-6465 Protein denotes CD40
T5314 6470-6474 Protein denotes IL-4
T5315 6565-6583 Protein denotes anti-CD40 antibody
T5316 6705-6709 Protein denotes CD40
T6220 7598-7602 Protein denotes CD40
T6221 7614-7619 Protein denotes NF-κB
T6222 7624-7627 Protein denotes JNK
T6223 7628-7638 Positive_regulation denotes activation
T6224 7589-7597 Positive_regulation denotes mediates
T6225 7603-7613 Positive_regulation denotes stimulated
T6226 7628-7638 Positive_regulation denotes activation
T6227 7709-7713 Protein denotes CD40
T6228 7803-7807 Protein denotes CD40
T6229 7895-7900 Protein denotes CD154
T6230 7902-7906 Protein denotes CD40
T6231 7985-7990 Protein denotes NF-κB
T6232 7995-7998 Protein denotes JNK
T6233 7915-7924 Gene_expression denotes expressed
T6234 7915-7924 Gene_expression denotes expressed
T6235 7967-7977 Positive_regulation denotes activation
T6236 7967-7977 Positive_regulation denotes activation
T6237 8092-8097 Protein denotes CD154
T6238 8253-8257 Protein denotes CD40
T6239 8296-8301 Protein denotes NF-κB
T6240 8351-8363 Protein denotes IκB proteins
T6241 8371-8389 Protein denotes IκB kinase complex
T6242 8391-8394 Protein denotes IKK
T6243 8268-8278 Positive_regulation denotes Activation
T6244 8332-8347 Phosphorylation denotes phosphorylation
T6245 8332-8347 Phosphorylation denotes phosphorylation
T6246 8332-8347 Phosphorylation denotes phosphorylation
T6247 8415-8427 Protein denotes IκB proteins
T6248 8461-8475 Protein denotes NF-κB subunits
T6249 8448-8460 Positive_regulation denotes sequestering
T6250 8517-8520 Protein denotes IKK
T6251 8537-8540 Protein denotes IκB
T6252 8595-8600 Protein denotes NF-κB
T6253 8494-8509 Phosphorylation denotes Phosphorylation
T6254 8529-8536 Regulation denotes targets
T6255 8573-8584 Protein_catabolism denotes degradation
T6256 8614-8621 Entity denotes nucleus
T6257 8529-8536 Regulation denotes targets
T6258 8652-8656 Protein denotes CD40
T6259 8701-8705 Protein denotes IκBα
T6260 8666-8681 Phosphorylation denotes phosphorylation
T6261 8686-8697 Protein_catabolism denotes degradation
T6262 8747-8755 Negative_regulation denotes impaired
T6263 8747-8755 Negative_regulation denotes impaired
T6264 8657-8665 Positive_regulation denotes mediated
T6265 8657-8665 Positive_regulation denotes mediated
T6266 8851-8882 Protein denotes stress-activated protein kinase
T6267 8883-8886 Protein denotes JNK
T6268 8902-8906 Protein denotes CD40
T6269 8833-8843 Positive_regulation denotes activation
T6270 8833-8843 Positive_regulation denotes activation
T6271 8907-8917 Binding denotes engagement
T6272 8928-8932 Protein denotes CD40
T6273 8942-8945 Protein denotes JNK
T6274 9049-9052 Protein denotes JNK
T6275 8946-8956 Positive_regulation denotes activation
T6276 9009-9024 Phosphorylation denotes phosphorylation
T6277 8933-8941 Positive_regulation denotes mediated
T6278 8946-8956 Positive_regulation denotes activation
T6279 9054-9058 Protein denotes CD40
T6280 9081-9086 Protein denotes NF-κB
T6281 9091-9094 Protein denotes JNK
T6282 9226-9230 Protein denotes HOIP
T6283 9231-9241 Gene_expression denotes expression
T6284 9215-9222 Negative_regulation denotes absence
T8616 10395-10399 Protein denotes CD40
T8617 10544-10548 Protein denotes CD40
T8618 10644-10648 Protein denotes CD40
T8619 10672-10677 Protein denotes TRAF2
T8620 10741-10746 Protein denotes TRAF2
T8621 10627-10636 Binding denotes recruited
T8622 10787-10792 Protein denotes TRAF2
T8623 10813-10818 Protein denotes cIAP1
T8624 10823-10828 Protein denotes cIAP2
T8625 10863-10867 Protein denotes HOIP
T8626 10871-10876 Protein denotes TNFR1
T8627 10848-10859 Binding denotes recruitment
T8628 10954-10958 Protein denotes CD40
T8629 11030-11034 Protein denotes cIAP
T8630 10929-10940 Binding denotes association
T8631 11017-11026 Negative_regulation denotes inhibitor
T8632 11167-11179 Protein denotes SMAC peptide
T8633 11215-11220 Protein denotes cIAP1
T8634 11241-11245 Protein denotes CD40
T8635 11294-11298 Protein denotes CD40
T8636 11221-11231 Binding denotes associated
T8637 11193-11200 Negative_regulation denotes reduced
T8638 11457-11461 Protein denotes CD40
T8639 11564-11582 Protein denotes anti-HOIP antibody
T8640 11462-11481 Binding denotes immunoprecipitation
T8641 11550-11560 Binding denotes recognized
T8642 11398-11406 Negative_regulation denotes decrease
T8643 11616-11628 Protein denotes SMAC peptide
T8644 11797-11801 Protein denotes CD40
T8645 11922-11935 Protein denotes cIAP proteins
T8646 12007-12011 Protein denotes CD40
T8647 14591-14595 Protein denotes CD40
T8648 14619-14623 Protein denotes CD40
T8649 14729-14733 Protein denotes CD40
T8650 14817-14822 Protein denotes TRAF2
T8651 14824-14829 Protein denotes TRAF3
T8652 14831-14837 Protein denotes IKKα/β
T8653 14868-14873 Protein denotes TRAF2
T8654 14875-14880 Protein denotes TRAF3
T8655 14886-14891 Protein denotes cIAP1
T8656 14895-14899 Protein denotes CD40
T8657 15075-15079 Protein denotes CD40
T8658 15040-15051 Binding denotes association
T8659 15040-15051 Binding denotes association
T8660 15040-15051 Binding denotes association
T8661 15094-15109 Protein denotes IKKα/β and IKKγ
T8662 15133-15137 Protein denotes CD40
T8663 15119-15129 Gene_expression denotes detectable
T8664 15119-15129 Gene_expression denotes detectable
T8665 15221-15244 Protein denotes IKKα/β and IKKγ in CD40
T8666 15400-15403 Protein denotes IKK
T8667 15468-15472 Protein denotes HOIP
T8668 15404-15415 Binding denotes recruitment
T8669 15473-15483 Gene_expression denotes expression
T8670 15457-15464 Negative_regulation denotes absence
T8671 15550-15555 Protein denotes NF-κB
T8672 15582-15586 Protein denotes CD40
T8673 15531-15542 Binding denotes recruitment
T8674 15651-15655 Protein denotes IKKγ
T8675 15667-15671 Protein denotes CD40
T8676 15830-15834 Protein denotes IKKγ
T8677 15958-15968 Protein denotes mouse IKKγ
T8678 15987-15991 Protein denotes CD40
T8679 16359-16363 Protein denotes IKKγ
T8680 16367-16371 Protein denotes CD40
T8681 16381-16385 Protein denotes HOIP
T8682 16344-16355 Binding denotes recruitment
T8683 16372-16380 Positive_regulation denotes requires
T8684 16467-16471 Protein denotes CD40
T8685 16532-16550 Protein denotes lambda phosphatase
T8686 16673-16677 Protein denotes CD40
T8687 16689-16693 Protein denotes IKKγ
T8688 16657-16664 Negative_regulation denotes reduced
T8689 16906-16910 Protein denotes CD40
T8690 16922-16926 Protein denotes IKKγ
T8691 16911-16921 Binding denotes associated
T8692 16988-17003 Phosphorylation denotes phosphorylation
T8693 17082-17086 Protein denotes IKKγ
T8694 17092-17096 Protein denotes CD40
T8695 17067-17078 Binding denotes association
T15300 2161-2166 Protein denotes Rnf31
T15301 2202-2211 Protein denotes HOIP gene
T15302 2443-2448 Protein denotes Rnf31
T15303 2520-2547 Protein denotes neomycin phosphotransferase
T15304 2549-2553 Protein denotes NeoR
T15305 2507-2516 Localization denotes insertion
T15306 2507-2516 Localization denotes insertion
T15307 2782-2785 Protein denotes Cre
T15308 2811-2831 Protein denotes NeoR coding sequence
T15309 2795-2803 Negative_regulation denotes deletion
T15310 2772-2777 Negative_regulation denotes allow
T15311 2786-2794 Positive_regulation denotes mediated
T15312 2921-2934 Protein denotes NeoR sequence
T15313 2905-2913 Negative_regulation denotes deletion
T15314 3071-3083 Protein denotes HOIP protein
T15315 3151-3156 Protein denotes Rnf31
T15316 3084-3094 Gene_expression denotes expression
T15317 3059-3067 Negative_regulation denotes decrease
T15318 3291-3295 Protein denotes HOIP
T15644 4763-4767 Protein denotes CD40
T15645 4777-4781 Protein denotes CD80
T15646 4782-4794 Positive_regulation denotes upregulation
T15647 4798-4807 Negative_regulation denotes defective
T15648 4768-4776 Positive_regulation denotes mediated
T15649 4873-4897 Protein denotes isotype control antibody
T15650 4913-4917 Protein denotes CD40
T15651 4970-4974 Protein denotes CD80
T15652 5020-5024 Protein denotes CD80
T15653 5070-5094 Protein denotes isotype control antibody
T15654 4956-4966 Gene_expression denotes expression
T15655 5206-5210 Protein denotes CD80
T15656 5211-5223 Positive_regulation denotes upregulation
T15657 5187-5195 Negative_regulation denotes reversed
T16092 6799-6803 Protein denotes CD40
T16093 7090-7094 Protein denotes CD40
T16094 7101-7125 Protein denotes isotype control antibody
T16095 7127-7130 Protein denotes iso
T16096 7149-7153 Protein denotes IL-4
T16097 7271-7286 Protein denotes GLε transcripts
T16098 7329-7346 Protein denotes Hprt1 transcripts
T16631 9287-9291 Protein denotes CD40
T16632 9314-9319 Protein denotes NF-κB
T16633 9324-9327 Protein denotes JNK
T16634 9300-9310 Positive_regulation denotes activation
T16635 9292-9299 Positive_regulation denotes induced
T16636 9300-9310 Positive_regulation denotes activation
T16637 9366-9370 Protein denotes CD40
T16638 9656-9661 Protein denotes CD154
T16639 9662-9672 Gene_expression denotes expressing
T16640 9782-9787 Protein denotes CD154
T16641 9774-9781 Negative_regulation denotes lacking
T16642 9895-9907 Protein denotes Phospho-IκBα
T16643 9909-9914 Protein denotes pIκBα
T16644 9928-9931 Protein denotes JNK
T16645 9933-9937 Protein denotes pJNK
T16646 9982-9986 Protein denotes IκBα
T16647 9997-10000 Protein denotes JNK
T16648 10021-10024 Protein denotes JNK
T16649 10046-10049 Protein denotes p46
T16650 10054-10066 Protein denotes p54 isoforms
T16651 10036-10045 Binding denotes recognize
T16652 10036-10045 Binding denotes recognize
T16653 10069-10079 Protein denotes IκBα blots
T16654 10103-10108 Protein denotes actin
T17868 12073-12076 Protein denotes IKK
T17869 12096-12100 Protein denotes CD40
T17870 12077-12088 Binding denotes recruitment
T17871 12207-12212 Protein denotes cIAP1
T17872 12216-12220 Protein denotes CD40
T17873 12192-12203 Binding denotes recruitment
T17874 12184-12191 Negative_regulation denotes reduces
T17875 12598-12607 Protein denotes anti-CD40
T17876 12614-12638 Protein denotes isotype control antibody
T17877 12847-12852 Protein denotes cIAP1
T17878 12854-12859 Protein denotes TRAF2
T17879 12861-12866 Protein denotes TRAF3
T17880 12993-12997 Protein denotes CD40
T17881 13251-13269 Protein denotes anti-CD40 antibody
T17882 13307-13311 Protein denotes CD40
T17883 13313-13318 Protein denotes TRAF2
T17884 13320-13325 Protein denotes TRAF3
T17885 13327-13332 Protein denotes cIAP1
T17886 13334-13338 Protein denotes HOIP
T17887 13340-13346 Protein denotes IKKα/β
T17888 13186-13204 Binding denotes immunoprecipitated
T17889 13425-13437 Protein denotes IKK proteins
T17890 13443-13447 Protein denotes CD40
T17891 13406-13421 Binding denotes coprecipitation
T17892 13393-13401 Positive_regulation denotes required
T17893 13652-13686 Protein denotes HOIP-dependent recruitment of IKKγ
T17894 13690-13694 Protein denotes CD40
T17895 13851-13855 Protein denotes IKKγ
T17896 13889-13893 Protein denotes IKKγ
T17897 14087-14093 Protein denotes BP tag
T17898 14095-14099 Protein denotes IKKγ
T17899 14118-14123 Protein denotes TRAF2
T17900 14129-14141 Protein denotes anti-CD40 IP
T17901 14177-14190 Protein denotes λ phosphatase
T17902 14383-14388 Protein denotes TRAF3
R3082 T3953 T3954 themeOf Rnf31,disruption
R3087 T3956 T3957 themeOf CD40,interaction
R3088 T3958 T3964 themeOf HOIP,recruitment
R3089 T3959 T3964 themeOf CD40,recruitment
R3090 T3961 T3965 themeOf HOIP mutant,overexpression
R3091 T3963 T3967 themeOf NF-κB,activation
R3092 T3965 T3966 themeOf overexpression,overexpression
R3093 T3976 T3977 themeOf HOIP protein,expression
R3094 T3979 T3981 themeOf Rnf31 gene,disruption
R3095 T3980 T3982 themeOf HOIP protein,expression
R3096 T3976 T3978 themeOf HOIP protein,undetectable
R4084 T5262 T5264 themeOf CD80,upregulation
R4085 T5263 T5265 themeOf germline epsilon,transcription
R4086 T5265 T5266 themeOf transcription,activation
R4087 T5266 T5267 themeOf activation,required
R4088 T5268 T5270 themeOf CD40,Engagement
R4089 T5269 T5271 themeOf CD80,expression
R4090 T5270 T5272 causeOf Engagement,upregulates
R4091 T5271 T5272 themeOf expression,upregulates
R4092 T5274 T5276 themeOf CD80,upregulation
R4093 T5275 T5277 themeOf CD40,engagement
R4094 T5278 T5280 causeOf CD40,upregulation
R4095 T5279 T5280 themeOf CD80,upregulation
R4096 T5280 T5281 themeOf upregulation,reduced
R4097 T5282 T5285 themeOf CD80,upregulation
R4098 T5283 T5286 themeOf CD80,upregulation
R4099 T5284 T5287 themeOf Rnf31,disruption
R4100 T5286 T5288 themeOf upregulation,defect
R4101 T5292 T5296 themeOf IgM,expressing
R4102 T5295 T5297 themeOf IgE,switch
R4103 T5298 T5302 themeOf IgE,induced
R4104 T5300 T5301 themeOf CD40 signals,combination
R4105 T5301 T5302 causeOf combination,induced
R4106 T5303 T5305 themeOf IgE,production
R4107 T5305 T5306 themeOf production,necessary
R4108 T5309 T5311 themeOf Anti-CD40 antibody,stimulation
R4785 T6220 T6225 causeOf CD40,stimulated
R4786 T6221 T6223 themeOf NF-κB,activation
R4787 T6223 T6224 themeOf activation,mediates
R4788 T6223 T6225 themeOf activation,stimulated
R4789 T6225 T6226 themeOf stimulated,activation
R4790 T6229 T6233 themeOf CD154,expressed
R4791 T6230 T6234 themeOf CD40,expressed
R4792 T6231 T6235 themeOf NF-κB,activation
R4793 T6232 T6236 themeOf JNK,activation
R4794 T6239 T6243 themeOf NF-κB,Activation
R4795 T6240 T6244 themeOf IκB proteins,phosphorylation
R4796 T6241 T6245 themeOf IκB kinase complex,phosphorylation
R4797 T6242 T6246 themeOf IKK,phosphorylation
R4798 T6247 T6249 causeOf IκB proteins,sequestering
R4799 T6248 T6249 themeOf NF-κB subunits,sequestering
R4800 T6250 T6253 themeOf IKK,Phosphorylation
R4801 T6251 T6254 themeOf IκB,targets
R4802 T6251 T6255 themeOf IκB,degradation
R4803 T6253 T6254 causeOf Phosphorylation,targets
R4804 T6253 T6257 causeOf Phosphorylation,targets
R4805 T6255 T6257 themeOf degradation,targets
R4806 T6258 T6264 causeOf CD40,mediated
R4807 T6258 T6265 causeOf CD40,mediated
R4808 T6259 T6260 themeOf IκBα,phosphorylation
R4809 T6259 T6261 themeOf IκBα,degradation
R4810 T6260 T6262 themeOf phosphorylation,impaired
R4811 T6260 T6264 themeOf phosphorylation,mediated
R4812 T6261 T6263 themeOf degradation,impaired
R4813 T6261 T6265 themeOf degradation,mediated
R4814 T6266 T6269 themeOf stress-activated protein kinase,activation
R4815 T6267 T6270 themeOf JNK,activation
R4816 T6268 T6271 themeOf CD40,engagement
R4817 T6272 T6277 causeOf CD40,mediated
R4818 T6273 T6275 themeOf JNK,activation
R4819 T6274 T6276 themeOf JNK,phosphorylation
R4820 T6275 T6277 themeOf activation,mediated
R4821 T6277 T6278 themeOf mediated,activation
R4822 T6282 T6283 themeOf HOIP,expression
R4823 T6283 T6284 themeOf expression,absence
R6724 T8625 T8627 themeOf HOIP,recruitment
R6744 T8626 T8627 themeOf TNFR1,recruitment
R6745 T8628 T8630 themeOf CD40,association
R6746 T8629 T8631 themeOf cIAP,inhibitor
R6747 T8633 T8636 themeOf cIAP1,associated
R6750 T8634 T8636 themeOf CD40,associated
R6751 T8618 T8621 themeOf CD40,recruited
R6752 T8636 T8637 themeOf associated,reduced
R6753 T8638 T8640 themeOf CD40,immunoprecipitation
R6755 T8639 T8641 themeOf anti-HOIP antibody,recognized
R6759 T8640 T8642 themeOf immunoprecipitation,decrease
R6761 T8653 T8658 themeOf TRAF2,association
R6775 T8654 T8659 themeOf TRAF3,association
R6781 T8655 T8660 themeOf cIAP1,association
R6786 T8657 T8658 themeOf CD40,association
R6790 T8657 T8659 themeOf CD40,association
R6792 T8657 T8660 themeOf CD40,association
R6793 T8661 T8663 themeOf IKKα/β and IKKγ,detectable
R6794 T8662 T8664 themeOf CD40,detectable
R6797 T8666 T8668 themeOf IKK,recruitment
R6802 T8667 T8669 themeOf HOIP,expression
R6803 T8669 T8670 themeOf expression,absence
R6804 T8671 T8673 themeOf NF-κB,recruitment
R6809 T8672 T8673 themeOf CD40,recruitment
R6812 T8679 T8682 themeOf IKKγ,recruitment
R6815 T8680 T8682 themeOf CD40,recruitment
R6817 T8681 T8683 themeOf HOIP,requires
R6819 T8682 T8683 causeOf recruitment,requires
R6820 T8687 T8688 themeOf IKKγ,reduced
R6824 T8689 T8691 themeOf CD40,associated
R6828 T8690 T8691 themeOf IKKγ,associated
R6831 T8690 T8692 themeOf IKKγ,phosphorylation
R6832 T8693 T8695 themeOf IKKγ,association
R6838 T8694 T8695 themeOf CD40,association
R12133 T15303 T15305 themeOf neomycin phosphotransferase,insertion
R12134 T15304 T15306 themeOf NeoR,insertion
R12135 T15307 T15311 causeOf Cre,mediated
R12136 T15308 T15309 themeOf NeoR coding sequence,deletion
R12137 T15309 T15310 themeOf deletion,allow
R12138 T15309 T15311 themeOf deletion,mediated
R12139 T15312 T15313 themeOf NeoR sequence,deletion
R12140 T15314 T15316 themeOf HOIP protein,expression
R12141 T15316 T15317 themeOf expression,decrease
R12390 T15644 T15648 causeOf CD40,mediated
R12391 T15645 T15646 themeOf CD80,upregulation
R12392 T15646 T15647 themeOf upregulation,defective
R12393 T15646 T15648 themeOf upregulation,mediated
R12394 T15651 T15654 themeOf CD80,expression
R12395 T15655 T15656 themeOf CD80,upregulation
R12396 T15656 T15657 themeOf upregulation,reversed
R13124 T16631 T16635 causeOf CD40,induced
R13125 T16632 T16634 themeOf NF-κB,activation
R13126 T16634 T16635 themeOf activation,induced
R13127 T16635 T16636 themeOf induced,activation
R13128 T16638 T16639 themeOf CD154,expressing
R13129 T16640 T16641 themeOf CD154,lacking
R13130 T16649 T16651 themeOf p46,recognize
R13131 T16650 T16652 themeOf p54 isoforms,recognize
R14086 T17868 T17870 themeOf IKK,recruitment
R14087 T17869 T17870 themeOf CD40,recruitment
R14088 T17871 T17873 themeOf cIAP1,recruitment
R14089 T17872 T17873 themeOf CD40,recruitment
R14090 T17873 T17874 themeOf recruitment,reduces
R14091 T17881 T17888 themeOf anti-CD40 antibody,immunoprecipitated
R14092 T17889 T17891 themeOf IKK proteins,coprecipitation
R14093 T17890 T17891 themeOf CD40,coprecipitation
R14094 T17891 T17892 themeOf coprecipitation,required

events-check-again

Id Subject Object Predicate Lexical cue Negation Speculation
T3983 23-27 Protein denotes HOIP
T3984 28-37 Negative_regulation denotes deficient
T3985 59-69 Negative_regulation denotes disruption
T3986 73-78 Protein denotes Rnf31
T3987 98-102 Protein denotes HOIP
T3988 147-151 Protein denotes HOIP
T3989 155-164 Binding denotes recruited
T3990 300-311 Binding denotes interaction
T3991 315-319 Protein denotes CD40
T3992 325-329 Protein denotes HOIP
T3993 424-435 Binding denotes recruitment
T3994 439-443 Protein denotes HOIP
T3995 447-451 Protein denotes CD40
T3996 456-461 Protein denotes TRAF2
T3997 462-471 Regulation denotes dependent
T3998 511-515 Protein denotes HOIP
T3999 543-547 Protein denotes CD40
T4000 617-621 Protein denotes HOIP
T4001 638-642 Protein denotes CD40
T4002 722-726 Protein denotes HOIP
T4003 730-734 Protein denotes CD40
T4004 785-792 Negative_regulation denotes disrupt
T4005 811-815 Protein denotes HOIP
T4006 920-924 Protein denotes CD40
T4007 1131-1136 Protein denotes Rnf31
T4008 1156-1160 Protein denotes HOIP
T4009 1386-1395 Negative_regulation denotes disrupted
T4010 1396-1401 Protein denotes Rnf31
T4011 1465-1474 Negative_regulation denotes disrupted
T4012 1475-1480 Protein denotes Rnf31
T4013 1569-1584 Protein denotes Cre recombinase
T4014 1707-1712 Protein denotes Rnf31
T4015 1718-1727 Negative_regulation denotes disrupted
T4016 1797-1801 Protein denotes HOIP
T4017 1810-1820 Gene_expression denotes expression true true
T4018 2059-2063 Protein denotes HOIP
T4019 2064-2073 Negative_regulation denotes deficient
T4020 3438-3442 Protein denotes HOIP
T4021 3443-3452 Negative_regulation denotes deficient
T4022 3471-3481 Negative_regulation denotes disruption
T4023 3489-3494 Protein denotes Rnf31
T4024 3506-3510 Protein denotes HOIP
T4025 3511-3520 Negative_regulation denotes deficient
T4026 3597-3601 Protein denotes HOIP
T4027 3608-3617 Positive_regulation denotes restoring
T4028 3618-3622 Protein denotes HOIP
T4029 3631-3641 Gene_expression denotes expression
T4030 3684-3688 Protein denotes HOIP
T4031 3689-3702 Positive_regulation denotes reconstituted
T4032 3810-3814 Protein denotes HOIP
T4033 3815-3824 Negative_regulation denotes deficient
T5317 3913-3917 Protein denotes HOIP
T5318 3921-3929 Positive_regulation denotes required
T5319 3921-3929 Positive_regulation denotes required
T5320 3934-3938 Protein denotes CD40
T5321 3939-3947 Positive_regulation denotes mediated
T5322 3939-3947 Positive_regulation denotes mediated
T5323 3948-3952 Protein denotes CD80
T5324 3953-3965 Positive_regulation denotes upregulation
T5325 3970-3980 Positive_regulation denotes activation
T5326 3984-4000 Protein denotes germline epsilon
T5327 4001-4014 Transcription denotes transcription
T5328 4015-4025 Binding denotes Engagement
T5329 4029-4033 Protein denotes CD40
T5330 4045-4056 Positive_regulation denotes upregulates
T5331 4057-4067 Gene_expression denotes expression
T5332 4071-4075 Protein denotes CD80
T5333 4228-4232 Protein denotes HOIP
T5334 4236-4240 Protein denotes CD40
T5335 4267-4279 Positive_regulation denotes upregulation
T5336 4283-4287 Protein denotes CD80
T5337 4288-4295 Positive_regulation denotes induced true
T5338 4299-4309 Binding denotes engagement
T5339 4313-4317 Protein denotes CD40
T5340 4338-4342 Protein denotes HOIP
T5341 4358-4362 Protein denotes HOIP
T5342 4363-4376 Positive_regulation denotes reconstituted
T5343 4433-4437 Protein denotes CD40
T5344 4438-4448 Positive_regulation denotes stimulated
T5345 4449-4461 Positive_regulation denotes upregulation
T5346 4465-4469 Protein denotes CD80
T5347 4487-4494 Negative_regulation denotes reduced
T5348 4502-4506 Protein denotes HOIP
T5349 4507-4516 Negative_regulation denotes deficient
T5350 4544-4556 Positive_regulation denotes upregulation
T5351 4560-4564 Protein denotes CD80
T5352 4581-4585 Protein denotes HOIP
T5353 4586-4599 Positive_regulation denotes reconstituted
T5354 4627-4633 Negative_regulation denotes defect
T5355 4637-4641 Protein denotes CD80
T5356 4642-4654 Positive_regulation denotes upregulation
T5357 4662-4666 Protein denotes HOIP
T5358 4667-4676 Negative_regulation denotes deficient
T5359 4687-4690 Positive_regulation denotes due
T5360 4698-4708 Negative_regulation denotes disruption
T5361 4712-4717 Protein denotes Rnf31
T5362 5337-5341 Protein denotes CD40
T5363 5762-5766 Protein denotes IL-4
T5364 5771-5775 Protein denotes CD40
T5365 5977-5981 Protein denotes HOIP
T5366 6094-6098 Protein denotes CD40
T5367 6118-6122 Protein denotes IL-4
T5368 6138-6148 Transcription denotes production true
T5369 6152-6168 Protein denotes germline epsilon
T5370 6170-6173 Protein denotes GLε
T5371 6251-6255 Protein denotes IL-4
T5372 6256-6263 Positive_regulation denotes induced
T5373 6264-6267 Protein denotes GLε
T5374 6268-6281 Transcription denotes transcription
T5375 6352-6355 Protein denotes GLε
T5376 6356-6366 Gene_expression denotes expression
T5377 6384-6388 Protein denotes HOIP
T5378 6389-6398 Negative_regulation denotes deficient
T5379 6470-6474 Protein denotes IL-4
T5380 6493-6496 Protein denotes GLε
T5381 6497-6507 Gene_expression denotes expression
T5382 6513-6520 Positive_regulation denotes induced
T5383 6524-6528 Protein denotes HOIP
T5384 6529-6542 Positive_regulation denotes reconstituted
T5385 6607-6611 Protein denotes IL-4
T5386 6655-6659 Protein denotes HOIP
T5387 6705-6709 Protein denotes CD40
T6285 7584-7588 Protein denotes HOIP
T6286 7598-7602 Protein denotes CD40
T6287 7677-7681 Protein denotes HOIP
T6288 7709-7713 Protein denotes CD40
T6289 7770-7774 Protein denotes HOIP
T6290 7803-7807 Protein denotes CD40
T6291 7895-7900 Protein denotes CD154
T6292 7902-7913 Protein denotes CD40 ligand
T6293 7915-7924 Gene_expression denotes expressed
T6294 8066-8070 Protein denotes CD40
T6295 8081-8091 Binding denotes associated
T6296 8092-8097 Protein denotes CD154
T6297 8432-8443 Regulation denotes responsible
T6298 8448-8460 Negative_regulation denotes sequestering
T6299 8461-8475 Protein denotes NF-κB subunits
T6300 8652-8656 Protein denotes CD40
T6301 8657-8665 Positive_regulation denotes mediated
T6302 8657-8665 Positive_regulation denotes mediated
T6303 8666-8681 Phosphorylation denotes phosphorylation
T6304 8686-8697 Protein_catabolism denotes degradation
T6305 8701-8705 Protein denotes IκBα
T6306 8709-8713 Protein denotes HOIP
T6307 8714-8723 Negative_regulation denotes deficient
T6308 8747-8755 Negative_regulation denotes impaired
T6309 8747-8755 Negative_regulation denotes impaired
T6310 8902-8906 Protein denotes CD40
T6311 8907-8917 Binding denotes engagement
T6312 8928-8932 Protein denotes CD40
T6313 8960-8964 Protein denotes HOIP
T6314 8965-8974 Negative_regulation denotes deficient
T6315 9054-9058 Protein denotes CD40
T6316 9098-9102 Protein denotes HOIP
T6317 9103-9116 Positive_regulation denotes reconstituted
T6318 9226-9230 Protein denotes HOIP
T6319 9231-9241 Gene_expression denotes expression true
T8589 10368-10372 Protein denotes CD40
T8590 10395-10399 Protein denotes CD40
T8591 10452-10456 Protein denotes HOIP
T8592 10457-10466 Negative_regulation denotes deficient
T8593 10488-10492 Protein denotes HOIP
T8594 10619-10623 Protein denotes HOIP
T8595 10627-10636 Binding denotes recruited
T8596 10672-10677 Protein denotes TRAF2
T8597 10678-10687 Regulation denotes dependent
T8598 10712-10716 Protein denotes HOIP
T8599 10717-10737 Regulation denotes functions downstream true
T8600 10741-10746 Protein denotes TRAF2
T8601 10787-10792 Protein denotes TRAF2
T8602 10793-10803 Binding denotes associated
T8603 10793-10803 Binding denotes associated
T8604 10813-10818 Protein denotes cIAP1
T8605 10823-10828 Protein denotes cIAP2
T8606 10836-10840 Regulation denotes role
T8607 10836-10840 Regulation denotes role
T8608 10836-10840 Regulation denotes role
T8609 10836-10840 Regulation denotes role
T8610 10848-10859 Binding denotes recruitment
T8611 10848-10859 Binding denotes recruitment
T8612 10863-10867 Protein denotes HOIP
T8696 10871-10876 Protein denotes TNFR1
T8697 10881-10885 Protein denotes CD40
T8698 10929-10940 Binding denotes association
T8699 10944-10948 Protein denotes HOIP
T8700 10954-10958 Protein denotes CD40
T8701 10979-10986 Regulation denotes altered true
T8702 11193-11200 Negative_regulation denotes reduced
T8703 11215-11220 Protein denotes cIAP1
T8704 11221-11231 Binding denotes associated
T8705 11360-11368 Positive_regulation denotes resulted
T8706 11360-11368 Positive_regulation denotes resulted
T8707 11398-11406 Negative_regulation denotes decrease
T8708 11434-11438 Protein denotes HOIP
T8709 11444-11453 Binding denotes recovered
T8710 11457-11461 Protein denotes CD40
T8711 11506-11514 Positive_regulation denotes increase
T8712 11550-11560 Binding denotes recognized
T8713 11637-11642 Regulation denotes alter true
T8714 11677-11681 Protein denotes HOIP
T8715 11767-11773 Regulation denotes alters
T8716 11797-11801 Protein denotes CD40
T8717 11802-11812 Binding denotes associated
T8718 11813-11817 Protein denotes HOIP
T8719 11922-11926 Protein denotes cIAP
T8720 11936-11945 Regulation denotes influence
T8721 11936-11945 Regulation denotes influence
T8722 11950-11961 Binding denotes recruitment
T8723 11966-11997 Protein_modification denotes post-translational modification
T8724 12007-12011 Protein denotes CD40
T8725 12012-12022 Binding denotes associated
T8726 12023-12027 Protein denotes HOIP
T8727 14511-14515 Protein denotes HOIP
T8728 14519-14530 Regulation denotes responsible true
T8729 14539-14550 Binding denotes recruitment
T8730 14591-14595 Protein denotes CD40
T8731 14649-14653 Protein denotes HOIP
T8732 14654-14663 Negative_regulation denotes deficient
T8733 14668-14672 Protein denotes HOIP
T8734 14673-14686 Positive_regulation denotes reconstituted
T8735 14801-14805 Protein denotes HOIP
T8736 14817-14822 Protein denotes TRAF2
T8737 14824-14829 Protein denotes TRAF3
T8738 14831-14835 Protein denotes IKKα
T8739 14836-14837 Protein denotes β
T8740 14843-14847 Protein denotes IKKγ
T8741 14868-14873 Protein denotes TRAF2
T8742 14875-14880 Protein denotes TRAF3
T8743 14886-14891 Protein denotes cIAP1
T8744 14895-14899 Protein denotes CD40
T8745 14924-14928 Protein denotes HOIP
T8746 15011-15015 Protein denotes HOIP
T8747 15023-15031 Positive_regulation denotes required true
T8748 15023-15031 Positive_regulation denotes required true
T8749 15023-15031 Positive_regulation denotes required true
T8750 15040-15051 Binding denotes association
T8751 15040-15051 Binding denotes association
T8752 15040-15051 Binding denotes association
T8753 15075-15079 Protein denotes CD40
T8754 15094-15098 Protein denotes IKKα
T8755 15099-15100 Protein denotes β
T8756 15105-15109 Protein denotes IKKγ
T8757 15133-15137 Protein denotes CD40
T8758 15172-15176 Protein denotes HOIP
T8759 15177-15186 Negative_regulation denotes deficient
T8760 15221-15225 Protein denotes IKKα
T8761 15226-15227 Protein denotes β
T8762 15232-15236 Protein denotes IKKγ
T8763 15240-15244 Protein denotes CD40
T8764 15269-15273 Protein denotes HOIP
T8765 15274-15287 Positive_regulation denotes reconstituted
T8766 15457-15464 Negative_regulation denotes absence
T8767 15468-15472 Protein denotes HOIP
T8768 15473-15483 Gene_expression denotes expression
T8769 15510-15514 Protein denotes HOIP
T8770 15651-15655 Protein denotes IKKγ
T8771 15667-15671 Protein denotes CD40
T8772 15830-15834 Protein denotes IKKγ
T8773 15964-15968 Protein denotes IKKγ
T8774 15987-15991 Protein denotes CD40
T8775 16229-16233 Protein denotes IKKγ
T8776 16298-16308 Gene_expression denotes expressing
T8777 16324-16328 Protein denotes IKKγ
T8778 16344-16355 Binding denotes recruitment
T8779 16359-16363 Protein denotes IKKγ
T8780 16367-16371 Protein denotes CD40
T8781 16372-16380 Positive_regulation denotes requires
T8782 16381-16385 Protein denotes HOIP
T8783 16459-16463 Protein denotes IKKγ
T8784 16467-16471 Protein denotes CD40
T8785 16673-16677 Protein denotes CD40
T8786 16678-16688 Binding denotes associated
T8787 16689-16693 Protein denotes IKKγ
T8788 16906-16910 Protein denotes CD40
T8789 16911-16921 Binding denotes associated
T8790 16922-16926 Protein denotes IKKγ
T8791 16988-17003 Phosphorylation denotes phosphorylation
T8792 17042-17046 Protein denotes HOIP
T8793 17050-17058 Positive_regulation denotes required
T8794 17067-17078 Binding denotes association
T8795 17082-17086 Protein denotes IKKγ
T8796 17092-17096 Protein denotes CD40
T8797 17129-17136 Positive_regulation denotes coupled
T8798 17140-17172 Protein_modification denotes post-translational modifications
T8799 17176-17180 Protein denotes IKKγ
T15319 2125-2129 Protein denotes HOIP
T15320 2161-2166 Protein denotes Rnf31
T15321 2202-2206 Protein denotes HOIP
T15322 2443-2448 Protein denotes Rnf31
T15323 2579-2583 Protein denotes HOIP
T15324 3016-3020 Protein denotes HOIP
T15325 3021-3030 Negative_regulation denotes deficient
T15326 3032-3036 Protein denotes HOIP
T15327 3059-3067 Negative_regulation denotes decrease
T15328 3071-3075 Protein denotes HOIP
T15329 3084-3094 Gene_expression denotes expression
T15330 3115-3124 Positive_regulation denotes following
T15331 3125-3135 Negative_regulation denotes disruption
T15332 3151-3156 Protein denotes Rnf31
T15333 3158-3162 Protein denotes HOIP
T15334 3168-3172 Protein denotes HOIP
T15335 3173-3183 Gene_expression denotes expression
T15336 3291-3295 Protein denotes HOIP
T15337 3343-3347 Protein denotes HOIP
T15658 4763-4767 Protein denotes CD40
T15659 4768-4776 Positive_regulation denotes mediated
T15660 4777-4781 Protein denotes CD80
T15661 4782-4794 Positive_regulation denotes upregulation
T15662 4798-4807 Negative_regulation denotes defective
T15663 4811-4815 Protein denotes HOIP
T15664 4816-4825 Negative_regulation denotes deficient
T15665 4956-4966 Gene_expression denotes expression true
T15666 4970-4974 Protein denotes CD80
T15667 5118-5122 Protein denotes HOIP
T15668 5123-5132 Negative_regulation denotes deficient
T15669 5134-5138 Protein denotes HOIP
T15670 5182-5186 Protein denotes HOIP
T15671 5187-5195 Negative_regulation denotes reversed
T15672 5196-5205 Negative_regulation denotes defective
T15673 5206-5210 Protein denotes CD80
T15674 5211-5223 Positive_regulation denotes upregulation
T16099 6765-6768 Protein denotes GLε
T16100 6769-6782 Transcription denotes transcription
T16101 6786-6795 Negative_regulation denotes defective
T16102 6799-6803 Protein denotes CD40
T16103 6815-6819 Protein denotes HOIP
T16104 6820-6829 Negative_regulation denotes deficient
T16105 6848-6852 Protein denotes HOIP
T16106 6853-6862 Negative_regulation denotes deficient
T16107 6931-6935 Protein denotes HOIP
T16108 6965-6969 Protein denotes HOIP
T16109 6970-6979 Negative_regulation denotes deficient
T16110 7004-7008 Protein denotes HOIP
T16111 7030-7034 Protein denotes HOIP
T16112 7040-7044 Protein denotes HOIP
T16113 7149-7153 Protein denotes IL-4
T16114 7271-7274 Protein denotes GLε
T16115 7275-7286 Transcription denotes transcripts true
T16116 7329-7334 Protein denotes Hprt1
T16117 7335-7346 Transcription denotes transcripts
T16118 7561-7565 Protein denotes HOIP
T16119 7566-7575 Negative_regulation denotes deficient
T16655 9287-9291 Protein denotes CD40
T16656 9344-9348 Protein denotes HOIP
T16657 9349-9358 Negative_regulation denotes deficient
T16658 9366-9370 Protein denotes CD40
T16659 9407-9411 Protein denotes HOIP
T16660 9412-9421 Negative_regulation denotes deficient
T16661 9541-9545 Protein denotes HOIP
T16662 9546-9555 Negative_regulation denotes deficient
T16663 9607-9611 Protein denotes HOIP
T16664 9613-9617 Protein denotes HOIP
T16665 9623-9627 Protein denotes HOIP
T16666 9656-9661 Protein denotes CD154
T16667 9662-9672 Gene_expression denotes expressing
T16668 9774-9781 Negative_regulation denotes lacking
T16669 9782-9787 Protein denotes CD154
T16670 9895-9907 Protein denotes Phospho-IκBα
T16671 9909-9914 Protein denotes pIκBα
T16672 9982-9986 Protein denotes IκBα
T16673 10069-10073 Protein denotes IκBα
T16674 10287-10291 Protein denotes HOIP
T16675 10292-10301 Negative_regulation denotes deficient
T17903 12135-12139 Protein denotes HOIP
T17904 12140-12149 Negative_regulation denotes deficient
T17905 12184-12191 Negative_regulation denotes reduces
T17906 12192-12203 Binding denotes recruitment
T17907 12207-12212 Protein denotes cIAP1
T17908 12216-12220 Protein denotes CD40
T17909 12229-12235 Regulation denotes modify true
T17910 12236-12240 Protein denotes HOIP
T17911 12241-12252 Binding denotes recruitment
T17912 12847-12852 Protein denotes cIAP1
T17913 12854-12859 Protein denotes TRAF2
T17914 12861-12866 Protein denotes TRAF3
T17915 12872-12876 Protein denotes HOIP
T17916 12993-12997 Protein denotes CD40
T17917 13068-13072 Protein denotes HOIP
T17918 13073-13082 Negative_regulation denotes deficient
T17919 13171-13175 Protein denotes HOIP
T17920 13307-13311 Protein denotes CD40
T17921 13313-13318 Protein denotes TRAF2
T17922 13320-13325 Protein denotes TRAF3
T17923 13327-13332 Protein denotes cIAP1
T17924 13334-13338 Protein denotes HOIP
T17925 13340-13344 Protein denotes IKKα
T17926 13345-13346 Protein denotes β
T17927 13352-13356 Protein denotes IKKγ
T17928 13373-13377 Protein denotes HOIP
T17929 13378-13388 Gene_expression denotes expression
T17930 13443-13447 Protein denotes CD40
T17931 13606-13610 Protein denotes HOIP
T17932 13611-13620 Negative_regulation denotes deficient
T17933 13652-13656 Protein denotes HOIP
T17934 13657-13666 Regulation denotes dependent true
T17935 13667-13678 Binding denotes recruitment
T17936 13682-13686 Protein denotes IKKγ
T17937 13690-13694 Protein denotes CD40
T17938 13712-13716 Protein denotes HOIP
T17939 13717-13726 Negative_regulation denotes deficient
T17940 13728-13732 Protein denotes HOIP
T17941 13851-13855 Protein denotes IKKγ
T17942 13880-13884 Protein denotes pMIP
T17943 13889-13893 Protein denotes IKKγ
T17944 14095-14099 Protein denotes IKKγ
T17945 14118-14123 Protein denotes TRAF2
T17946 14383-14388 Protein denotes TRAF3
T17947 14393-14397 Protein denotes HOIP
R3097 T4012 T4011 themeOf Rnf31,disrupted
R3098 T4014 T4015 themeOf Rnf31,disrupted
R3099 T4016 T4017 themeOf HOIP,expression
R3100 T3983 T3984 themeOf HOIP,deficient
R3101 T4018 T4019 themeOf HOIP,deficient
R3102 T3986 T3985 themeOf Rnf31,disruption
R3103 T4020 T4021 themeOf HOIP,deficient
R3104 T3987 T3986 equivalentTo HOIP,Rnf31
R3105 T3988 T3989 themeOf HOIP,recruited
R3106 T4023 T4022 themeOf Rnf31,disruption
R3107 T3991 T3990 themeOf CD40,interaction
R3108 T3992 T3990 themeOf HOIP,interaction
R3109 T4024 T4025 themeOf HOIP,deficient
R3110 T3993 T3997 themeOf recruitment,dependent
R3111 T3994 T3993 themeOf HOIP,recruitment
R3112 T3995 T3993 themeOf CD40,recruitment
R3113 T3996 T3997 causeOf TRAF2,dependent
R3114 T4028 T4029 themeOf HOIP,expression
R3115 T4029 T4027 themeOf expression,restoring
R3116 T4030 T4031 themeOf HOIP,reconstituted
R3117 T4005 T4004 themeOf HOIP,disrupt
R3118 T4008 T4007 equivalentTo HOIP,Rnf31
R3119 T4010 T4009 themeOf Rnf31,disrupted
R3120 T4032 T4033 themeOf HOIP,deficient
R4109 T5317 T5319 causeOf HOIP,required
R4110 T5317 T5318 causeOf HOIP,required
R4111 T5321 T5318 themeOf mediated,required
R4112 T5322 T5319 themeOf mediated,required
R4113 T5323 T5324 themeOf CD80,upregulation
R4114 T5324 T5322 themeOf upregulation,mediated
R4115 T5325 T5321 themeOf activation,mediated
R4116 T5326 T5327 themeOf germline epsilon,transcription
R4117 T5327 T5325 themeOf transcription,activation
R4118 T5328 T5330 causeOf Engagement,upregulates
R4119 T5329 T5328 themeOf CD40,Engagement
R4120 T5331 T5330 themeOf expression,upregulates
R4121 T5332 T5331 themeOf CD80,expression
R4122 T5335 T5337 themeOf upregulation,induced
R4123 T5336 T5335 themeOf CD80,upregulation
R4124 T5338 T5337 causeOf engagement,induced
R4125 T5339 T5338 themeOf CD40,engagement
R4126 T5341 T5342 themeOf HOIP,reconstituted
R4127 T5343 T5344 causeOf CD40,stimulated
R4128 T5345 T5344 themeOf upregulation,stimulated
R4129 T5345 T5347 themeOf upregulation,reduced
R4130 T5346 T5345 themeOf CD80,upregulation
R4131 T5348 T5349 themeOf HOIP,deficient
R4132 T5349 T5347 causeOf deficient,reduced
R4133 T5351 T5350 themeOf CD80,upregulation
R4134 T5352 T5353 themeOf HOIP,reconstituted
R4135 T5354 T5359 themeOf defect,due
R4136 T5355 T5356 themeOf CD80,upregulation
R4137 T5356 T5354 themeOf upregulation,defect
R4138 T5357 T5358 themeOf HOIP,deficient
R4139 T5358 T5354 causeOf deficient,defect
R4140 T5360 T5359 causeOf disruption,due
R4141 T5361 T5360 themeOf Rnf31,disruption
R4142 T5369 T5368 themeOf germline epsilon,production
R4143 T5370 T5369 equivalentTo GLε,germline epsilon
R4144 T5373 T5374 themeOf GLε,transcription
R4145 T5374 T5372 themeOf transcription,induced
R4146 T5375 T5376 themeOf GLε,expression
R4147 T5377 T5378 themeOf HOIP,deficient
R4148 T5380 T5381 themeOf GLε,expression
R4149 T5381 T5382 themeOf expression,induced
R4150 T5383 T5384 themeOf HOIP,reconstituted
R4824 T6291 T6293 themeOf CD154,expressed
R4825 T6292 T6291 equivalentTo CD40 ligand,CD154
R4826 T6296 T6295 themeOf CD154,associated
R4827 T6298 T6297 themeOf sequestering,responsible
R4828 T6299 T6298 themeOf NF-κB subunits,sequestering
R4829 T6300 T6301 causeOf CD40,mediated
R4830 T6300 T6302 causeOf CD40,mediated
R4831 T6301 T6308 themeOf mediated,impaired
R4832 T6302 T6309 themeOf mediated,impaired
R4833 T6303 T6302 themeOf phosphorylation,mediated
R4834 T6304 T6301 themeOf degradation,mediated
R4835 T6305 T6304 themeOf IκBα,degradation
R4836 T6305 T6303 themeOf IκBα,phosphorylation
R4837 T6306 T6307 themeOf HOIP,deficient
R4838 T6310 T6311 themeOf CD40,engagement
R4839 T6313 T6314 themeOf HOIP,deficient
R4840 T6316 T6317 themeOf HOIP,reconstituted
R4841 T6318 T6319 themeOf HOIP,expression
R6722 T8591 T8592 themeOf HOIP,deficient
R6725 T8594 T8595 themeOf HOIP,recruited
R6726 T8595 T8597 themeOf recruited,dependent
R6727 T8596 T8597 causeOf TRAF2,dependent
R6728 T8598 T8599 themeOf HOIP,functions downstream
R6729 T8600 T8599 causeOf TRAF2,functions downstream
R6730 T8601 T8602 themeOf TRAF2,associated
R6731 T8601 T8603 themeOf TRAF2,associated
R6732 T8604 T8602 themeOf cIAP1,associated
R6733 T8604 T8609 causeOf cIAP1,role
R6734 T8604 T8608 causeOf cIAP1,role
R6735 T8605 T8603 themeOf cIAP2,associated
R6736 T8605 T8606 causeOf cIAP2,role
R6737 T8605 T8607 causeOf cIAP2,role
R6738 T8610 T8609 themeOf recruitment,role
R6739 T8610 T8606 themeOf recruitment,role
R6740 T8611 T8608 themeOf recruitment,role
R6741 T8611 T8607 themeOf recruitment,role
R6742 T8612 T8610 themeOf HOIP,recruitment
R6743 T8612 T8611 themeOf HOIP,recruitment
R6754 T8696 T8610 themeOf TNFR1,recruitment
R6756 T8697 T8611 themeOf CD40,recruitment
R6757 T8698 T8701 themeOf association,altered
R6758 T8699 T8698 themeOf HOIP,association
R6760 T8700 T8698 themeOf CD40,association
R6762 T8703 T8704 themeOf cIAP1,associated
R6763 T8704 T8702 themeOf associated,reduced
R6764 T8707 T8706 themeOf decrease,resulted
R6765 T8708 T8707 themeOf HOIP,decrease
R6766 T8708 T8711 themeOf HOIP,increase
R6767 T8708 T8709 themeOf HOIP,recovered
R6768 T8708 T8712 themeOf HOIP,recognized
R6769 T8710 T8709 themeOf CD40,recovered
R6770 T8711 T8705 themeOf increase,resulted
R6771 T8714 T8713 themeOf HOIP,alter
R6772 T8716 T8717 themeOf CD40,associated
R6773 T8718 T8715 themeOf HOIP,alters
R6774 T8718 T8717 themeOf HOIP,associated
R6776 T8719 T8721 causeOf cIAP,influence
R6777 T8719 T8720 causeOf cIAP,influence
R6778 T8722 T8721 themeOf recruitment,influence
R6779 T8723 T8720 themeOf post-translational modification,influence
R6780 T8724 T8725 themeOf CD40,associated
R6782 T8726 T8722 themeOf HOIP,recruitment
R6783 T8726 T8723 themeOf HOIP,post-translational modification
R6784 T8726 T8725 themeOf HOIP,associated
R6785 T8727 T8728 causeOf HOIP,responsible
R6787 T8729 T8728 themeOf recruitment,responsible
R6788 T8730 T8729 themeOf CD40,recruitment
R6789 T8731 T8732 themeOf HOIP,deficient
R6791 T8733 T8734 themeOf HOIP,reconstituted
R6795 T8741 T8750 themeOf TRAF2,association
R6796 T8742 T8752 themeOf TRAF3,association
R6798 T8743 T8751 themeOf cIAP1,association
R6799 T8746 T8749 causeOf HOIP,required
R6800 T8746 T8748 causeOf HOIP,required
R6801 T8746 T8747 causeOf HOIP,required
R6805 T8750 T8749 themeOf association,required
R6806 T8751 T8747 themeOf association,required
R6807 T8752 T8748 themeOf association,required
R6808 T8753 T8750 themeOf CD40,association
R6810 T8753 T8752 themeOf CD40,association
R6811 T8753 T8751 themeOf CD40,association
R6813 T8758 T8759 themeOf HOIP,deficient
R6814 T8764 T8765 themeOf HOIP,reconstituted
R6816 T8767 T8768 themeOf HOIP,expression
R6818 T8768 T8766 themeOf expression,absence
R6821 T8777 T8776 themeOf IKKγ,expressing
R6822 T8778 T8781 themeOf recruitment,requires
R6823 T8779 T8778 themeOf IKKγ,recruitment
R6825 T8780 T8778 themeOf CD40,recruitment
R6826 T8782 T8781 causeOf HOIP,requires
R6827 T8785 T8786 themeOf CD40,associated
R6829 T8787 T8786 themeOf IKKγ,associated
R6830 T8788 T8789 themeOf CD40,associated
R6833 T8790 T8789 themeOf IKKγ,associated
R6834 T8790 T8791 themeOf IKKγ,phosphorylation
R6835 T8792 T8793 causeOf HOIP,required
R6836 T8794 T8793 themeOf association,required
R6837 T8794 T8797 causeOf association,coupled
R6839 T8795 T8794 themeOf IKKγ,association
R6840 T8796 T8794 themeOf CD40,association
R6841 T8798 T8797 themeOf post-translational modifications,coupled
R6842 T8799 T8798 themeOf IKKγ,post-translational modifications
R12142 T15324 T15325 themeOf HOIP,deficient
R12143 T15327 T15330 themeOf decrease,following
R12144 T15328 T15329 themeOf HOIP,expression
R12145 T15329 T15327 themeOf expression,decrease
R12146 T15331 T15330 causeOf disruption,following
R12147 T15332 T15331 themeOf Rnf31,disruption
R12148 T15334 T15335 themeOf HOIP,expression
R12397 T15658 T15659 causeOf CD40,mediated
R12398 T15659 T15662 themeOf mediated,defective
R12399 T15660 T15661 themeOf CD80,upregulation
R12400 T15661 T15659 themeOf upregulation,mediated
R12401 T15663 T15664 themeOf HOIP,deficient
R12402 T15666 T15665 themeOf CD80,expression
R12403 T15667 T15668 themeOf HOIP,deficient
R12404 T15672 T15671 themeOf defective,reversed
R12405 T15673 T15674 themeOf CD80,upregulation
R12406 T15674 T15672 themeOf upregulation,defective
R12727 T16099 T16100 themeOf GLε,transcription
R12728 T16100 T16101 themeOf transcription,defective
R12729 T16103 T16104 themeOf HOIP,deficient
R12730 T16105 T16106 themeOf HOIP,deficient
R12731 T16108 T16109 themeOf HOIP,deficient
R12732 T16114 T16115 themeOf GLε,transcripts
R12733 T16116 T16117 themeOf Hprt1,transcripts
R12734 T16118 T16119 themeOf HOIP,deficient
R13132 T16656 T16657 themeOf HOIP,deficient
R13133 T16659 T16660 themeOf HOIP,deficient
R13134 T16661 T16662 themeOf HOIP,deficient
R13135 T16666 T16667 themeOf CD154,expressing
R13136 T16669 T16668 themeOf CD154,lacking
R13137 T16671 T16670 equivalentTo pIκBα,Phospho-IκBα
R13138 T16674 T16675 themeOf HOIP,deficient
R14095 T17903 T17904 themeOf HOIP,deficient
R14096 T17906 T17905 themeOf recruitment,reduces
R14097 T17907 T17906 themeOf cIAP1,recruitment
R14098 T17908 T17906 themeOf CD40,recruitment
R14099 T17910 T17911 themeOf HOIP,recruitment
R14100 T17911 T17909 themeOf recruitment,modify
R14101 T17917 T17918 themeOf HOIP,deficient
R14102 T17928 T17929 themeOf HOIP,expression
R14103 T17931 T17932 themeOf HOIP,deficient
R14104 T17933 T17934 causeOf HOIP,dependent
R14105 T17935 T17934 themeOf recruitment,dependent
R14106 T17936 T17935 themeOf IKKγ,recruitment
R14107 T17937 T17935 themeOf CD40,recruitment
R14108 T17938 T17939 themeOf HOIP,deficient

bionlp-st-ge-2016-coref

Id Subject Object Predicate Lexical cue
T8182 11542-11549 Anaphor denotes species
T8183 11434-11438 Antecedent denotes HOIP
T8184 15055-15069 Anaphor denotes these proteins
T8185 14868-14873 Antecedent denotes TRAF2
T8186 14875-14880 Antecedent denotes TRAF3
T8187 14886-14891 Antecedent denotes cIAP1
T8188 17115-17125 Anaphor denotes this event
T8189 17067-17078 Antecedent denotes association
R6476 T8182 T8183 boundBy species,HOIP
R6478 T8184 T8185 boundBy these proteins,TRAF2
R6479 T8184 T8186 boundBy these proteins,TRAF3
R6480 T8184 T8187 boundBy these proteins,cIAP1
R6482 T8188 T8189 boundBy this event,association

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T3341 9-19 NN denotes Generation
T3342 20-22 IN denotes of
T3343 23-37 JJ denotes HOIP-deficient
T3344 38-39 NN denotes B
T3345 40-45 NN denotes cells
T3346 46-49 IN denotes via
T3347 50-58 VB denotes targeted
T3348 59-69 NN denotes disruption
T3349 70-72 IN denotes of
T3350 73-78 NN denotes Rnf31
T3351 78-79 -COMMA- denotes ,
T3352 80-83 DT denotes the
T3353 84-88 NN denotes gene
T3354 89-97 VB denotes encoding
T3355 98-102 NN denotes HOIP
T3356 103-105 IN denotes In
T3357 106-109 PRP-DOLLAR- denotes our
T3358 110-118 JJ denotes previous
T3359 119-124 NN denotes study
T3360 124-125 -COMMA- denotes ,
T3361 126-128 PRP denotes we
T3362 129-141 VB denotes demonstrated
T3363 142-146 IN denotes that
T3364 147-151 NN denotes HOIP
T3365 152-154 VB denotes is
T3366 155-164 VB denotes recruited
T3367 165-167 TO denotes to
T3368 168-171 DT denotes the
T3369 172-176 NN denotes CD40
T3370 177-186 NN denotes signaling
T3371 187-194 NN denotes complex
T3372 195-197 IN denotes in
T3373 198-201 CD denotes two
T3374 202-207 NN denotes mouse
T3375 208-209 NN denotes B
T3376 210-214 NN denotes cell
T3377 215-220 NN denotes lines
T3378 221-222 -LRB- denotes [
T3379 222-223 CD denotes 6
T3380 223-224 -RRB- denotes ]
T3381 226-233 JJ denotes Similar
T3382 234-241 NN denotes results
T3383 242-246 VB denotes were
T3384 247-255 VB denotes obtained
T3385 256-260 IN denotes with
T3386 261-266 NN denotes mouse
T3387 267-278 NN denotes splenocytes
T3388 278-279 -COMMA- denotes ,
T3389 280-290 VB denotes indicating
T3390 291-295 IN denotes that
T3391 296-299 DT denotes the
T3392 300-311 NN denotes interaction
T3393 312-314 IN denotes of
T3394 315-319 NN denotes CD40
T3395 320-324 IN denotes with
T3396 325-329 NN denotes HOIP
T3397 330-332 VB denotes is
T3398 333-336 RB denotes not
T3399 337-344 VB denotes limited
T3400 345-347 TO denotes to
T3401 348-359 VB denotes transformed
T3402 360-361 NN denotes B
T3403 362-366 NN denotes cell
T3404 367-372 NN denotes lines
T3405 373-374 -LRB- denotes (
T3406 374-385 JJ denotes unpublished
T3407 386-398 NN denotes observations
T3408 398-399 -RRB- denotes )
T3409 401-403 PRP denotes We
T3410 404-408 RB denotes also
T3411 409-414 VB denotes found
T3412 415-419 IN denotes that
T3413 420-423 DT denotes the
T3414 424-435 NN denotes recruitment
T3415 436-438 IN denotes of
T3416 439-443 NN denotes HOIP
T3417 444-446 TO denotes to
T3418 447-451 NN denotes CD40
T3419 452-455 VB denotes was
T3420 456-471 JJ denotes TRAF2-dependent
T3421 472-475 CC denotes and
T3422 476-480 IN denotes that
T3423 481-495 NN denotes overexpression
T3424 496-498 IN denotes of
T3425 499-500 DT denotes a
T3426 501-510 VB denotes truncated
T3427 511-515 NN denotes HOIP
T3428 516-522 NN denotes mutant
T3429 523-532 RB denotes partially
T3430 533-542 VB denotes inhibited
T3431 543-556 JJ denotes CD40-mediated
T3432 557-562 NN denotes NF-κB
T3433 563-573 NN denotes activation
T3434 575-580 DT denotes These
T3435 581-588 NN denotes results
T3436 589-596 VB denotes support
T3437 597-600 DT denotes the
T3438 601-611 NN denotes hypothesis
T3439 612-616 IN denotes that
T3440 617-621 NN denotes HOIP
T3441 622-627 VB denotes plays
T3442 628-629 DT denotes a
T3443 630-634 NN denotes role
T3444 635-637 IN denotes in
T3445 638-642 NN denotes CD40
T3446 643-649 NN denotes signal
T3447 650-662 NN denotes transduction
T3448 664-666 TO denotes To
T3449 667-674 RB denotes further
T3450 675-681 VB denotes define
T3451 682-685 DT denotes the
T3452 686-690 NN denotes role
T3453 691-694 CC denotes and
T3454 695-703 VB denotes evaluate
T3455 704-707 DT denotes the
T3456 708-718 NN denotes importance
T3457 719-721 IN denotes of
T3458 722-726 NN denotes HOIP
T3459 727-729 IN denotes in
T3460 730-734 NN denotes CD40
T3461 735-744 NN denotes signaling
T3462 744-745 -COMMA- denotes ,
T3463 746-748 PRP denotes we
T3464 749-753 VB denotes used
T3465 754-761 JJ denotes somatic
T3466 762-766 NN denotes cell
T3467 767-771 NN denotes gene
T3468 772-781 VB denotes targeting
T3469 782-784 TO denotes to
T3470 785-792 VB denotes disrupt
T3471 793-796 DT denotes the
T3472 797-801 NN denotes gene
T3473 802-810 VB denotes encoding
T3474 811-815 NN denotes HOIP
T3475 816-818 IN denotes in
T3476 819-822 DT denotes the
T3477 823-828 NN denotes mouse
T3478 829-830 NN denotes B
T3479 831-835 NN denotes cell
T3480 836-840 NN denotes line
T3481 841-847 NN denotes A20.2J
T3482 849-853 DT denotes This
T3483 854-858 NN denotes cell
T3484 859-863 NN denotes line
T3485 864-867 VB denotes has
T3486 868-872 VB denotes been
T3487 873-885 RB denotes particularly
T3488 886-892 JJ denotes useful
T3489 893-895 IN denotes in
T3490 896-899 DT denotes the
T3491 900-916 NN denotes characterization
T3492 917-919 IN denotes of
T3493 920-924 NN denotes CD40
T3494 925-934 NN denotes signaling
T3495 935-945 NN denotes mechanisms
T3496 946-949 JJ denotes due
T3497 950-952 TO denotes to
T3498 953-956 DT denotes the
T3499 957-965 JJ denotes relative
T3500 966-970 NN denotes ease
T3501 971-975 IN denotes with
T3502 976-981 WDT denotes which
T3503 982-985 PRP-DOLLAR- denotes its
T3504 986-992 NN denotes genome
T3505 993-996 MD denotes can
T3506 997-999 VB denotes be
T3507 1000-1008 VB denotes modified
T3508 1009-1011 IN denotes by
T3509 1012-1022 JJ denotes homologous
T3510 1023-1036 NN denotes recombination
T3511 1037-1038 -LRB- denotes [
T3512 1038-1039 CD denotes 7
T3513 1039-1040 -RRB- denotes ]
T3514 1040-1041 -COMMA- denotes ,
T3515 1042-1043 -LRB- denotes [
T3516 1043-1044 CD denotes 8
T3517 1044-1045 -RRB- denotes ]
T3518 1045-1046 -COMMA- denotes ,
T3519 1047-1048 -LRB- denotes [
T3520 1048-1049 CD denotes 9
T3521 1049-1050 -RRB- denotes ]
T3522 1052-1054 PRP denotes We
T3523 1055-1059 VB denotes used
T3524 1060-1061 DT denotes a
T3525 1062-1071 VB denotes targeting
T3526 1072-1078 NN denotes vector
T3527 1079-1086 JJ denotes capable
T3528 1087-1089 IN denotes of
T3529 1090-1100 VB denotes undergoing
T3530 1101-1111 JJ denotes homologous
T3531 1112-1125 NN denotes recombination
T3532 1126-1130 IN denotes with
T3533 1131-1136 NN denotes Rnf31
T3534 1137-1138 -LRB- denotes (
T3535 1138-1141 DT denotes the
T3536 1142-1146 NN denotes gene
T3537 1147-1155 VB denotes encoding
T3538 1156-1160 NN denotes HOIP
T3539 1160-1161 -RRB- denotes )
T3540 1162-1164 TO denotes to
T3541 1165-1172 VB denotes disrupt
T3542 1173-1176 DT denotes the
T3543 1177-1183 VB denotes coding
T3544 1184-1192 NN denotes sequence
T3545 1193-1195 IN denotes of
T3546 1196-1199 DT denotes the
T3547 1200-1204 NN denotes gene
T3548 1205-1207 IN denotes in
T3549 1208-1212 NN denotes exon
T3550 1213-1214 CD denotes 5
T3551 1215-1216 -LRB- denotes (
T3552 1216-1220 NNP denotes Fig.
T3553 1221-1223 NN denotes 1A
T3554 1223-1224 -RRB- denotes )
T3555 1226-1235 VB denotes Following
T3556 1236-1248 NN denotes introduction
T3557 1249-1251 IN denotes of
T3558 1252-1255 DT denotes the
T3559 1256-1262 NN denotes vector
T3560 1262-1263 -COMMA- denotes ,
T3561 1264-1267 DT denotes the
T3562 1268-1286 JJ denotes neomycin-resistant
T3563 1287-1293 NN denotes clones
T3564 1294-1298 WDT denotes that
T3565 1299-1304 VB denotes arose
T3566 1305-1309 VB denotes were
T3567 1310-1318 VB denotes screened
T3568 1319-1321 IN denotes by
T3569 1322-1325 NN denotes PCR
T3570 1326-1339 NN denotes amplification
T3571 1340-1342 IN denotes of
T3572 1343-1350 JJ denotes genomic
T3573 1351-1354 NN denotes DNA
T3574 1355-1357 TO denotes to
T3575 1358-1366 VB denotes identify
T3576 1367-1372 NN denotes cells
T3577 1373-1383 VB denotes containing
T3578 1384-1385 DT denotes a
T3579 1386-1395 VB denotes disrupted
T3580 1396-1401 NN denotes Rnf31
T3581 1402-1408 NN denotes allele
T3582 1410-1412 TO denotes To
T3583 1413-1419 VB denotes remove
T3584 1420-1423 DT denotes the
T3585 1424-1434 JJ denotes selectable
T3586 1435-1441 NN denotes marker
T3587 1442-1446 NN denotes gene
T3588 1447-1455 NN denotes cassette
T3589 1456-1460 IN denotes from
T3590 1461-1464 DT denotes the
T3591 1465-1474 VB denotes disrupted
T3592 1475-1480 NN denotes Rnf31
T3593 1481-1487 NN denotes allele
T3594 1487-1488 -COMMA- denotes ,
T3595 1489-1500 JJ denotes recombinant
T3596 1501-1505 NN denotes cell
T3597 1506-1511 NN denotes lines
T3598 1512-1516 VB denotes were
T3599 1517-1528 RB denotes transiently
T3600 1529-1540 VB denotes transfected
T3601 1541-1545 IN denotes with
T3602 1546-1547 DT denotes a
T3603 1548-1555 NN denotes plasmid
T3604 1556-1560 WDT denotes that
T3605 1561-1568 VB denotes encodes
T3606 1569-1572 NNP denotes Cre
T3607 1573-1584 NN denotes recombinase
T3608 1586-1590 DT denotes This
T3609 1591-1595 NN denotes step
T3610 1596-1603 VB denotes allowed
T3611 1604-1606 PRP denotes us
T3612 1607-1609 TO denotes to
T3613 1610-1617 VB denotes perform
T3614 1618-1619 DT denotes a
T3615 1620-1626 JJ denotes second
T3616 1627-1632 NN denotes round
T3617 1633-1635 IN denotes of
T3618 1636-1645 VB denotes targeting
T3619 1646-1649 CC denotes and
T3620 1650-1654 NN denotes drug
T3621 1655-1664 NN denotes selection
T3622 1664-1665 -COMMA- denotes ,
T3623 1666-1676 VB denotes generating
T3624 1677-1682 NN denotes cells
T3625 1683-1685 IN denotes in
T3626 1686-1691 WDT denotes which
T3627 1692-1696 DT denotes both
T3628 1697-1703 NN denotes copies
T3629 1704-1706 IN denotes of
T3630 1707-1712 NN denotes Rnf31
T3631 1713-1717 VB denotes were
T3632 1718-1727 VB denotes disrupted
T3633 1729-1732 CD denotes Two
T3634 1733-1744 JJ denotes independent
T3635 1745-1751 JJ denotes clonal
T3636 1752-1756 NN denotes cell
T3637 1757-1762 NN denotes lines
T3638 1763-1767 VB denotes were
T3639 1768-1774 VB denotes chosen
T3640 1775-1778 IN denotes for
T3641 1779-1786 JJ denotes further
T3642 1787-1795 NN denotes analysis
T3643 1797-1801 NN denotes HOIP
T3644 1802-1809 NN denotes protein
T3645 1810-1820 NN denotes expression
T3646 1821-1824 VB denotes was
T3647 1825-1837 JJ denotes undetectable
T3648 1838-1840 IN denotes in
T3649 1841-1845 DT denotes both
T3650 1846-1850 NN denotes cell
T3651 1851-1856 NN denotes lines
T3652 1856-1857 -COMMA- denotes ,
T3653 1858-1860 IN denotes as
T3654 1861-1871 VB denotes determined
T3655 1872-1874 IN denotes by
T3656 1875-1882 JJ denotes Western
T3657 1883-1887 NN denotes blot
T3658 1888-1896 NN denotes analysis
T3659 1897-1899 IN denotes of
T3660 1900-1904 NN denotes cell
T3661 1905-1912 NN denotes lysates
T3662 1913-1914 -LRB- denotes (
T3663 1914-1918 NNP denotes Fig.
T3664 1919-1921 NN denotes 1B
T3665 1921-1922 -RRB- denotes )
T3666 1922-1923 -COMMA- denotes ,
T3667 1924-1937 VB denotes demonstrating
T3668 1938-1942 IN denotes that
T3669 1943-1946 DT denotes the
T3670 1947-1956 VB denotes targeting
T3671 1957-1964 NN denotes process
T3672 1965-1968 VB denotes was
T3673 1969-1979 JJ denotes successful
T3674 1981-1983 IN denotes In
T3675 1984-1987 DT denotes the
T3676 1988-1992 NN denotes text
T3677 1993-1997 WDT denotes that
T3678 1998-2005 VB denotes follows
T3679 2005-2006 -COMMA- denotes ,
T3680 2007-2010 DT denotes the
T3681 2011-2014 CD denotes two
T3682 2015-2028 JJ denotes gene-targeted
T3683 2029-2033 NN denotes cell
T3684 2034-2039 NN denotes lines
T3685 2040-2043 VB denotes are
T3686 2044-2052 VB denotes referred
T3687 2053-2055 TO denotes to
T3688 2056-2058 IN denotes as
T3689 2059-2073 JJ denotes HOIP-deficient
T3690 2074-2079 NN denotes cells
T3691 3361-3363 TO denotes To
T3692 3364-3370 VB denotes enable
T3693 3371-3373 PRP denotes us
T3694 3374-3376 TO denotes to
T3695 3377-3384 VB denotes confirm
T3696 3385-3389 IN denotes that
T3697 3390-3393 DT denotes any
T3698 3394-3403 NN denotes signaling
T3699 3404-3406 CC denotes or
T3700 3407-3417 JJ denotes functional
T3701 3418-3425 NN denotes defects
T3702 3426-3434 VB denotes observed
T3703 3435-3437 IN denotes in
T3704 3438-3452 JJ denotes HOIP-deficient
T3705 3453-3458 NN denotes cells
T3706 3459-3463 VB denotes were
T3707 3464-3467 JJ denotes due
T3708 3468-3470 TO denotes to
T3709 3471-3481 NN denotes disruption
T3710 3482-3484 IN denotes of
T3711 3485-3488 DT denotes the
T3712 3489-3494 NN denotes Rnf31
T3713 3495-3499 NN denotes gene
T3714 3499-3500 -COMMA- denotes ,
T3715 3501-3505 DT denotes both
T3716 3506-3520 JJ denotes HOIP-deficient
T3717 3521-3525 NN denotes cell
T3718 3526-3531 NN denotes lines
T3719 3532-3536 VB denotes were
T3720 3537-3547 VB denotes transduced
T3721 3548-3552 IN denotes with
T3722 3553-3554 DT denotes a
T3723 3555-3565 NN denotes retrovirus
T3724 3566-3574 VB denotes encoding
T3725 3575-3586 JJ denotes FLAG-tagged
T3726 3587-3596 JJ denotes wild-type
T3727 3597-3601 NN denotes HOIP
T3728 3601-3602 -COMMA- denotes ,
T3729 3603-3607 RB denotes thus
T3730 3608-3617 VB denotes restoring
T3731 3618-3622 NN denotes HOIP
T3732 3623-3630 NN denotes protein
T3733 3631-3641 NN denotes expression
T3734 3642-3643 -LRB- denotes (
T3735 3643-3647 NN denotes Fig.
T3736 3648-3650 NN denotes 1B
T3737 3650-3651 -RRB- denotes )
T3738 3653-3658 DT denotes These
T3739 3659-3664 NN denotes cells
T3740 3665-3668 VB denotes are
T3741 3669-3677 VB denotes referred
T3742 3678-3680 TO denotes to
T3743 3681-3683 IN denotes as
T3744 3684-3702 JJ denotes HOIP-reconstituted
T3745 3703-3708 NN denotes cells
T3746 3710-3712 IN denotes In
T3747 3713-3716 DT denotes the
T3748 3717-3728 NN denotes experiments
T3749 3729-3733 WDT denotes that
T3750 3734-3740 VB denotes follow
T3751 3740-3741 -COMMA- denotes ,
T3752 3742-3751 NN denotes responses
T3753 3752-3754 IN denotes by
T3754 3755-3760 DT denotes these
T3755 3761-3766 NN denotes cells
T3756 3767-3771 VB denotes were
T3757 3772-3780 VB denotes compared
T3758 3781-3783 TO denotes to
T3759 3784-3789 DT denotes those
T3760 3790-3792 IN denotes of
T3761 3793-3799 NN denotes A20.2J
T3762 3800-3805 NN denotes cells
T3763 3806-3809 CC denotes and
T3764 3810-3824 JJ denotes HOIP-deficient
T3765 3825-3830 NN denotes cells
T3766 3831-3841 VB denotes transduced
T3767 3842-3846 IN denotes with
T3768 3847-3848 DT denotes a
T3769 3849-3859 JJ denotes retroviral
T3770 3860-3866 NN denotes vector
T3771 3867-3868 -LRB- denotes (
T3772 3868-3872 NN denotes pMIP
T3773 3872-3873 -RRB- denotes )
T3774 3874-3881 VB denotes lacking
T3775 3882-3883 DT denotes a
T3776 3884-3888 NN denotes cDNA
T3777 3889-3895 NN denotes insert
T3778 3896-3897 -LRB- denotes (
T3779 3897-3902 JJ denotes empty
T3780 3903-3909 NN denotes vector
T3781 3909-3910 -RRB- denotes )
T4639 3913-3917 NN denotes HOIP
T4640 3918-3920 VB denotes is
T4641 3921-3929 VB denotes required
T4642 3930-3933 IN denotes for
T4643 3934-3947 JJ denotes CD40-mediated
T4644 3948-3952 NN denotes CD80
T4645 3953-3965 NN denotes upregulation
T4646 3966-3969 CC denotes and
T4647 3970-3980 NN denotes activation
T4648 3981-3983 IN denotes of
T4649 3984-3992 NN denotes germline
T4650 3993-4000 NN denotes epsilon
T4651 4001-4014 NN denotes transcription
T4652 4015-4025 NN denotes Engagement
T4653 4026-4028 IN denotes of
T4654 4029-4033 NN denotes CD40
T4655 4034-4036 IN denotes on
T4656 4037-4038 NN denotes B
T4657 4039-4044 NN denotes cells
T4658 4045-4056 VB denotes upregulates
T4659 4057-4067 NN denotes expression
T4660 4068-4070 IN denotes of
T4661 4071-4075 NN denotes CD80
T4662 4076-4077 -LRB- denotes [
T4663 4077-4079 CD denotes 10
T4664 4079-4080 -RRB- denotes ]
T4665 4080-4081 -COMMA- denotes ,
T4666 4082-4083 DT denotes a
T4667 4084-4088 NN denotes cell
T4668 4089-4096 NN denotes surface
T4669 4097-4104 NN denotes protein
T4670 4105-4109 WDT denotes that
T4671 4110-4118 VB denotes promotes
T4672 4119-4129 NN denotes activation
T4673 4130-4132 IN denotes of
T4674 4133-4134 NN denotes T
T4675 4135-4140 NN denotes cells
T4676 4141-4152 VB denotes interacting
T4677 4153-4157 IN denotes with
T4678 4158-4159 NN denotes B
T4679 4160-4165 NN denotes cells
T4680 4166-4169 CC denotes and
T4681 4170-4175 JJ denotes other
T4682 4176-4179 NN denotes APC
T4683 4181-4183 TO denotes To
T4684 4184-4189 VB denotes begin
T4685 4190-4200 VB denotes evaluating
T4686 4201-4210 JJ denotes potential
T4687 4211-4224 NN denotes contributions
T4688 4225-4227 IN denotes of
T4689 4228-4232 NN denotes HOIP
T4690 4233-4235 TO denotes to
T4691 4236-4240 NN denotes CD40
T4692 4241-4250 NN denotes signaling
T4693 4250-4251 -COMMA- denotes ,
T4694 4252-4254 PRP denotes we
T4695 4255-4262 VB denotes assayed
T4696 4263-4266 DT denotes the
T4697 4267-4279 NN denotes upregulation
T4698 4280-4282 IN denotes of
T4699 4283-4287 NN denotes CD80
T4700 4288-4295 VB denotes induced
T4701 4296-4298 IN denotes by
T4702 4299-4309 NN denotes engagement
T4703 4310-4312 IN denotes of
T4704 4313-4317 NN denotes CD40
T4705 4318-4320 IN denotes on
T4706 4321-4329 JJ denotes parental
T4707 4330-4336 NN denotes A20.2J
T4708 4336-4337 -COMMA- denotes ,
T4709 4338-4352 JJ denotes HOIP-deficient
T4710 4352-4353 -COMMA- denotes ,
T4711 4354-4357 CC denotes and
T4712 4358-4376 JJ denotes HOIP-reconstituted
T4713 4377-4382 NN denotes cells
T4714 4383-4384 -LRB- denotes (
T4715 4384-4388 NN denotes Fig.
T4716 4389-4390 CD denotes 2
T4717 4390-4391 -RRB- denotes )
T4718 4393-4401 VB denotes Compared
T4719 4402-4404 TO denotes to
T4720 4405-4408 DT denotes the
T4721 4409-4417 JJ denotes parental
T4722 4418-4422 NN denotes cell
T4723 4423-4427 NN denotes line
T4724 4427-4428 -COMMA- denotes ,
T4725 4429-4432 DT denotes the
T4726 4433-4448 JJ denotes CD40-stimulated
T4727 4449-4461 NN denotes upregulation
T4728 4462-4464 IN denotes of
T4729 4465-4469 NN denotes CD80
T4730 4470-4473 VB denotes was
T4731 4474-4486 RB denotes dramatically
T4732 4487-4494 VB denotes reduced
T4733 4495-4497 IN denotes in
T4734 4498-4501 DT denotes all
T4735 4502-4516 JJ denotes HOIP-deficient
T4736 4517-4522 NN denotes cells
T4737 4523-4529 VB denotes tested
T4738 4531-4533 IN denotes In
T4739 4534-4542 NN denotes contrast
T4740 4542-4543 -COMMA- denotes ,
T4741 4544-4556 NN denotes upregulation
T4742 4557-4559 IN denotes of
T4743 4560-4564 NN denotes CD80
T4744 4565-4568 VB denotes was
T4745 4569-4577 VB denotes restored
T4746 4578-4580 IN denotes in
T4747 4581-4599 JJ denotes HOIP-reconstituted
T4748 4600-4605 NN denotes cells
T4749 4605-4606 -COMMA- denotes ,
T4750 4607-4617 VB denotes confirming
T4751 4618-4622 IN denotes that
T4752 4623-4626 DT denotes the
T4753 4627-4633 NN denotes defect
T4754 4634-4636 IN denotes in
T4755 4637-4641 NN denotes CD80
T4756 4642-4654 NN denotes upregulation
T4757 4655-4657 IN denotes in
T4758 4658-4661 DT denotes the
T4759 4662-4676 JJ denotes HOIP-deficient
T4760 4677-4682 NN denotes cells
T4761 4683-4686 VB denotes was
T4762 4687-4690 JJ denotes due
T4763 4691-4693 TO denotes to
T4764 4694-4697 DT denotes the
T4765 4698-4708 NN denotes disruption
T4766 4709-4711 IN denotes of
T4767 4712-4717 NN denotes Rnf31
T4768 5337-5341 NN denotes CD40
T4769 5342-5349 NN denotes signals
T4770 5350-5352 IN denotes in
T4771 5353-5354 NN denotes B
T4772 5355-5360 NN denotes cells
T4773 5361-5364 MD denotes can
T4774 5365-5369 RB denotes also
T4775 5370-5380 VB denotes contribute
T4776 5381-5383 TO denotes to
T4777 5384-5387 DT denotes the
T4778 5388-5398 NN denotes activation
T4779 5399-5401 IN denotes of
T4780 5402-5405 NN denotes DNA
T4781 5406-5419 NN denotes recombination
T4782 5420-5422 IN denotes in
T4783 5423-5426 DT denotes the
T4784 5427-5441 NN denotes immunoglobulin
T4785 5442-5447 JJ denotes heavy
T4786 5448-5453 NN denotes chain
T4787 5454-5459 NN denotes locus
T4788 5460-5461 -LRB- denotes [
T4789 5461-5463 CD denotes 11
T4790 5463-5464 -RRB- denotes ]
T4791 5464-5465 -COMMA- denotes ,
T4792 5466-5467 -LRB- denotes [
T4793 5467-5469 CD denotes 12
T4794 5469-5470 -RRB- denotes ]
T4795 5472-5476 DT denotes This
T4796 5477-5484 NN denotes process
T4797 5484-5485 -COMMA- denotes ,
T4798 5486-5491 VB denotes known
T4799 5492-5494 IN denotes as
T4800 5495-5509 NN denotes immunoglobulin
T4801 5510-5515 NN denotes class
T4802 5516-5525 NN denotes switching
T4803 5526-5527 -LRB- denotes (
T4804 5527-5529 CC denotes or
T4805 5530-5537 NN denotes isotype
T4806 5538-5547 NN denotes switching
T4807 5547-5548 -RRB- denotes )
T4808 5548-5549 -COMMA- denotes ,
T4809 5550-5556 VB denotes allows
T4810 5557-5558 NN denotes B
T4811 5559-5564 NN denotes cells
T4812 5564-5565 -COMMA- denotes ,
T4813 5566-5575 RB denotes initially
T4814 5576-5586 VB denotes expressing
T4815 5587-5590 NN denotes IgM
T4816 5590-5591 -COMMA- denotes ,
T4817 5592-5594 TO denotes to
T4818 5595-5601 VB denotes switch
T4819 5602-5604 TO denotes to
T4820 5605-5608 NN denotes IgA
T4821 5608-5609 -COMMA- denotes ,
T4822 5610-5613 NN denotes IgG
T4823 5613-5614 -COMMA- denotes ,
T4824 5615-5617 CC denotes or
T4825 5618-5621 NN denotes IgE
T4826 5622-5631 VB denotes depending
T4827 5632-5634 IN denotes on
T4828 5635-5638 DT denotes the
T4829 5639-5644 NN denotes class
T4830 5645-5647 IN denotes of
T4831 5648-5656 NN denotes antibody
T4832 5657-5661 RB denotes most
T4833 5662-5673 JJ denotes appropriate
T4834 5674-5677 IN denotes for
T4835 5678-5679 DT denotes a
T4836 5680-5690 JJ denotes particular
T4837 5691-5697 JJ denotes immune
T4838 5698-5706 NN denotes response
T4839 5708-5717 VB denotes Switching
T4840 5718-5720 TO denotes to
T4841 5721-5724 NN denotes IgE
T4842 5725-5728 MD denotes can
T4843 5729-5731 VB denotes be
T4844 5732-5739 VB denotes induced
T4845 5740-5742 IN denotes by
T4846 5743-5746 DT denotes the
T4847 5747-5758 NN denotes combination
T4848 5759-5761 IN denotes of
T4849 5762-5766 NN denotes IL-4
T4850 5767-5770 CC denotes and
T4851 5771-5775 NN denotes CD40
T4852 5776-5783 NN denotes signals
T4853 5785-5788 DT denotes The
T4854 5789-5793 NN denotes gene
T4855 5794-5807 NN denotes rearrangement
T4856 5808-5817 JJ denotes necessary
T4857 5818-5821 IN denotes for
T4858 5822-5825 NN denotes IgE
T4859 5826-5836 NN denotes production
T4860 5837-5839 VB denotes is
T4861 5840-5848 VB denotes preceded
T4862 5849-5851 IN denotes by
T4863 5852-5855 DT denotes the
T4864 5856-5866 NN denotes production
T4865 5867-5869 IN denotes of
T4866 5870-5880 JJ denotes non-coding
T4867 5881-5884 NN denotes RNA
T4868 5885-5896 NN denotes transcripts
T4869 5897-5901 IN denotes from
T4870 5902-5910 NN denotes germline
T4871 5911-5920 NN denotes sequences
T4872 5921-5923 IN denotes in
T4873 5924-5927 DT denotes the
T4874 5928-5931 NN denotes IgE
T4875 5932-5937 JJ denotes heavy
T4876 5938-5943 NN denotes chain
T4877 5944-5949 NN denotes locus
T4878 5950-5951 -LRB- denotes [
T4879 5951-5953 CD denotes 13
T4880 5953-5954 -RRB- denotes ]
T4881 5956-5958 TO denotes To
T4882 5959-5968 VB denotes determine
T4883 5969-5976 IN denotes whether
T4884 5977-5981 NN denotes HOIP
T4885 5982-5984 VB denotes is
T4886 5985-5993 VB denotes required
T4887 5994-5996 IN denotes in
T4888 5997-6000 DT denotes the
T4889 6001-6011 NN denotes initiation
T4890 6012-6014 IN denotes of
T4891 6015-6018 DT denotes the
T4892 6019-6024 NN denotes class
T4893 6025-6034 NN denotes switching
T4894 6035-6042 NN denotes process
T4895 6042-6043 -COMMA- denotes ,
T4896 6044-6047 DT denotes the
T4897 6048-6056 JJ denotes relevant
T4898 6057-6058 NN denotes B
T4899 6059-6063 NN denotes cell
T4900 6064-6069 NN denotes lines
T4901 6070-6074 VB denotes were
T4902 6075-6085 VB denotes stimulated
T4903 6086-6093 IN denotes through
T4904 6094-6098 NN denotes CD40
T4905 6099-6101 IN denotes in
T4906 6102-6105 DT denotes the
T4907 6106-6114 NN denotes presence
T4908 6115-6117 IN denotes of
T4909 6118-6122 NN denotes IL-4
T4910 6123-6126 CC denotes and
T4911 6127-6133 VB denotes tested
T4912 6134-6137 IN denotes for
T4913 6138-6148 NN denotes production
T4914 6149-6151 IN denotes of
T4915 6152-6160 NN denotes germline
T4916 6161-6168 NN denotes epsilon
T4917 6169-6170 -LRB- denotes (
T4918 6170-6173 NN denotes GLε
T4919 6173-6174 -RRB- denotes )
T4920 6175-6186 NN denotes transcripts
T4921 6187-6188 -LRB- denotes (
T4922 6188-6192 NN denotes Fig.
T4923 6193-6194 CD denotes 3
T4924 6194-6195 -RRB- denotes )
T4925 6197-6206 JJ denotes Anti-CD40
T4926 6207-6215 NN denotes antibody
T4927 6216-6227 NN denotes stimulation
T4928 6228-6233 RB denotes alone
T4929 6234-6236 CC denotes or
T4930 6237-6245 RB denotes together
T4931 6246-6250 IN denotes with
T4932 6251-6255 NN denotes IL-4
T4933 6256-6263 VB denotes induced
T4934 6264-6267 NN denotes GLε
T4935 6268-6281 NN denotes transcription
T4936 6282-6284 IN denotes in
T4937 6285-6293 JJ denotes parental
T4938 6294-6300 NN denotes A20.2J
T4939 6301-6306 NN denotes cells
T4940 6307-6309 IN denotes as
T4941 6310-6318 VB denotes expected
T4942 6320-6322 IN denotes In
T4943 6323-6331 NN denotes contrast
T4944 6331-6332 -COMMA- denotes ,
T4945 6333-6337 RB denotes only
T4946 6338-6341 JJ denotes low
T4947 6342-6348 NN denotes levels
T4948 6349-6351 IN denotes of
T4949 6352-6355 NN denotes GLε
T4950 6356-6366 NN denotes expression
T4951 6367-6371 VB denotes were
T4952 6372-6380 VB denotes detected
T4953 6381-6383 IN denotes in
T4954 6384-6398 JJ denotes HOIP-deficient
T4955 6399-6404 NN denotes cells
T4956 6405-6415 VB denotes stimulated
T4957 6416-6420 IN denotes with
T4958 6421-6427 CC denotes either
T4959 6428-6437 JJ denotes anti-CD40
T4960 6438-6446 NN denotes antibody
T4961 6447-6452 RB denotes alone
T4962 6453-6455 CC denotes or
T4963 6456-6465 NN denotes anti-CD40
T4964 6466-6469 CC denotes and
T4965 6470-6474 NN denotes IL-4
T4966 6476-6482 JJ denotes Normal
T4967 6483-6489 NN denotes levels
T4968 6490-6492 IN denotes of
T4969 6493-6496 NN denotes GLε
T4970 6497-6507 NN denotes expression
T4971 6508-6512 VB denotes were
T4972 6513-6520 VB denotes induced
T4973 6521-6523 IN denotes in
T4974 6524-6542 JJ denotes HOIP-reconstituted
T4975 6543-6548 NN denotes cells
T4976 6549-6559 VB denotes stimulated
T4977 6560-6564 IN denotes with
T4978 6565-6574 JJ denotes anti-CD40
T4979 6575-6583 NN denotes antibody
T4980 6584-6589 RB denotes alone
T4981 6590-6592 CC denotes or
T4982 6593-6601 RB denotes together
T4983 6602-6606 IN denotes with
T4984 6607-6611 NN denotes IL-4
T4985 6613-6625 RB denotes Collectively
T4986 6625-6626 -COMMA- denotes ,
T4987 6627-6632 DT denotes these
T4988 6633-6637 NN denotes data
T4989 6638-6649 VB denotes demonstrate
T4990 6650-6654 IN denotes that
T4991 6655-6659 NN denotes HOIP
T4992 6660-6663 VB denotes has
T4993 6664-6665 DT denotes a
T4994 6666-6674 JJ denotes critical
T4995 6675-6679 NN denotes role
T4996 6680-6682 IN denotes in
T4997 6683-6691 JJ denotes cellular
T4998 6692-6701 NN denotes responses
T4999 6702-6704 TO denotes to
T5000 6705-6709 NN denotes CD40
T5001 6710-6719 NN denotes signaling
T5789 7584-7588 NN denotes HOIP
T5790 7589-7597 VB denotes mediates
T5791 7598-7613 JJ denotes CD40-stimulated
T5792 7614-7619 NN denotes NF-κB
T5793 7620-7623 CC denotes and
T5794 7624-7627 NN denotes JNK
T5795 7628-7638 NN denotes activation
T5796 7639-7642 DT denotes The
T5797 7643-7650 NN denotes results
T5798 7651-7660 VB denotes described
T5799 7661-7666 IN denotes above
T5800 7667-7671 VB denotes show
T5801 7672-7676 IN denotes that
T5802 7677-7681 NN denotes HOIP
T5803 7682-7687 VB denotes plays
T5804 7688-7690 DT denotes an
T5805 7691-7700 JJ denotes important
T5806 7701-7705 NN denotes role
T5807 7706-7708 IN denotes in
T5808 7709-7722 JJ denotes CD40-mediated
T5809 7723-7731 NN denotes effector
T5810 7732-7741 NN denotes functions
T5811 7742-7744 IN denotes of
T5812 7745-7746 NN denotes B
T5813 7747-7752 NN denotes cells
T5814 7754-7756 PRP denotes It
T5815 7757-7764 VB denotes follows
T5816 7765-7769 IN denotes that
T5817 7770-7774 NN denotes HOIP
T5818 7775-7777 VB denotes is
T5819 7778-7784 RB denotes likely
T5820 7785-7786 DT denotes a
T5821 7787-7790 JJ denotes key
T5822 7791-7799 NN denotes mediator
T5823 7800-7802 IN denotes of
T5824 7803-7807 NN denotes CD40
T5825 7808-7817 NN denotes signaling
T5826 7819-7821 TO denotes To
T5827 7822-7826 VB denotes test
T5828 7827-7831 DT denotes this
T5829 7832-7842 NN denotes hypothesis
T5830 7842-7843 -COMMA- denotes ,
T5831 7844-7846 PRP denotes we
T5832 7847-7857 VB denotes stimulated
T5833 7858-7864 NN denotes A20.2J
T5834 7865-7868 CC denotes and
T5835 7869-7883 JJ denotes HOIP-deficient
T5836 7884-7889 NN denotes cells
T5837 7890-7894 IN denotes with
T5838 7895-7900 NN denotes CD154
T5839 7901-7902 -LRB- denotes (
T5840 7902-7906 NN denotes CD40
T5841 7907-7913 NN denotes ligand
T5842 7913-7914 -RRB- denotes )
T5843 7915-7924 VB denotes expressed
T5844 7925-7927 IN denotes by
T5845 7928-7931 NN denotes HI5
T5846 7932-7938 NN denotes insect
T5847 7939-7944 NN denotes cells
T5848 7945-7946 -LRB- denotes [
T5849 7946-7947 CD denotes 7
T5850 7947-7948 -RRB- denotes ]
T5851 7948-7949 -COMMA- denotes ,
T5852 7950-7951 -LRB- denotes [
T5853 7951-7952 CD denotes 8
T5854 7952-7953 -RRB- denotes ]
T5855 7954-7957 CC denotes and
T5856 7958-7966 VB denotes measured
T5857 7967-7977 NN denotes activation
T5858 7978-7980 IN denotes of
T5859 7981-7984 DT denotes the
T5860 7985-7990 NN denotes NF-κB
T5861 7991-7994 CC denotes and
T5862 7995-7998 NN denotes JNK
T5863 7999-8007 NN denotes pathways
T5864 8007-8008 -COMMA- denotes ,
T5865 8009-8012 CD denotes two
T5866 8013-8015 IN denotes of
T5867 8016-8019 DT denotes the
T5868 8020-8025 JJ denotes major
T5869 8026-8041 JJ denotes transcriptional
T5870 8042-8052 NN denotes regulators
T5871 8053-8062 VB denotes activated
T5872 8063-8065 IN denotes by
T5873 8066-8070 NN denotes CD40
T5874 8071-8072 -LRB- denotes [
T5875 8072-8073 CD denotes 4
T5876 8073-8074 -RRB- denotes ]
T5877 8076-8091 JJ denotes Cell-associated
T5878 8092-8097 NN denotes CD154
T5879 8098-8101 VB denotes was
T5880 8102-8106 VB denotes used
T5881 8107-8109 IN denotes as
T5882 8110-8113 DT denotes the
T5883 8114-8122 NN denotes stimulus
T5884 8123-8125 IN denotes in
T5885 8126-8131 DT denotes these
T5886 8132-8143 NN denotes experiments
T5887 8144-8146 IN denotes as
T5888 8147-8149 PRP denotes it
T5889 8150-8159 RB denotes typically
T5890 8160-8168 VB denotes provides
T5891 8169-8173 RB denotes more
T5892 8174-8180 JJ denotes robust
T5893 8180-8181 -COMMA- denotes ,
T5894 8182-8185 CC denotes and
T5895 8186-8195 RB denotes therefore
T5896 8196-8200 RB denotes more
T5897 8201-8208 RB denotes readily
T5898 8209-8217 VB denotes detected
T5899 8217-8218 -COMMA- denotes ,
T5900 8219-8229 NN denotes activation
T5901 8230-8237 NN denotes signals
T5902 8238-8242 IN denotes than
T5903 8243-8247 VB denotes does
T5904 8248-8257 JJ denotes anti-CD40
T5905 8258-8266 NN denotes antibody
T5906 8268-8278 NN denotes Activation
T5907 8279-8281 IN denotes of
T5908 8282-8285 DT denotes the
T5909 8286-8295 JJ denotes canonical
T5910 8296-8301 NN denotes NF-κB
T5911 8302-8309 NN denotes pathway
T5912 8310-8312 VB denotes is
T5913 8313-8322 VB denotes initiated
T5914 8323-8327 IN denotes with
T5915 8328-8331 DT denotes the
T5916 8332-8347 NN denotes phosphorylation
T5917 8348-8350 IN denotes of
T5918 8351-8354 NN denotes IκB
T5919 8355-8363 NN denotes proteins
T5920 8364-8366 IN denotes by
T5921 8367-8370 DT denotes the
T5922 8371-8374 NN denotes IκB
T5923 8375-8381 NN denotes kinase
T5924 8382-8389 NN denotes complex
T5925 8390-8391 -LRB- denotes (
T5926 8391-8394 NN denotes IKK
T5927 8394-8395 -RRB- denotes )
T5928 8397-8399 IN denotes In
T5929 8400-8407 VB denotes resting
T5930 8408-8413 NN denotes cells
T5931 8413-8414 -COMMA- denotes ,
T5932 8415-8418 NN denotes IκB
T5933 8419-8427 NN denotes proteins
T5934 8428-8431 VB denotes are
T5935 8432-8443 JJ denotes responsible
T5936 8444-8447 IN denotes for
T5937 8448-8460 VB denotes sequestering
T5938 8461-8466 NN denotes NF-κB
T5939 8467-8475 NN denotes subunits
T5940 8476-8478 IN denotes in
T5941 8479-8482 DT denotes the
T5942 8483-8492 NN denotes cytoplasm
T5943 8494-8509 NN denotes Phosphorylation
T5944 8510-8512 IN denotes by
T5945 8513-8516 DT denotes the
T5946 8517-8520 NN denotes IKK
T5947 8521-8528 NN denotes complex
T5948 8529-8536 VB denotes targets
T5949 8537-8540 NN denotes IκB
T5950 8541-8549 NN denotes proteins
T5951 8550-8553 IN denotes for
T5952 8554-8568 NN denotes ubiquitination
T5953 8569-8572 CC denotes and
T5954 8573-8584 NN denotes degradation
T5955 8584-8585 -COMMA- denotes ,
T5956 8586-8594 VB denotes allowing
T5957 8595-8600 NN denotes NF-κB
T5958 8601-8603 TO denotes to
T5959 8604-8609 VB denotes enter
T5960 8610-8613 DT denotes the
T5961 8614-8621 NN denotes nucleus
T5962 8622-8625 CC denotes and
T5963 8626-8634 VB denotes activate
T5964 8635-8639 NN denotes gene
T5965 8640-8650 NN denotes expression
T5966 8652-8665 JJ denotes CD40-mediated
T5967 8666-8681 NN denotes phosphorylation
T5968 8682-8685 CC denotes and
T5969 8686-8697 NN denotes degradation
T5970 8698-8700 IN denotes of
T5971 8701-8705 NN denotes IκBα
T5972 8706-8708 IN denotes in
T5973 8709-8723 JJ denotes HOIP-deficient
T5974 8724-8729 NN denotes cells
T5975 8730-8733 VB denotes was
T5976 8734-8746 RB denotes dramatically
T5977 8747-8755 JJ denotes impaired
T5978 8756-8764 JJ denotes relative
T5979 8765-8767 TO denotes to
T5980 8768-8772 DT denotes that
T5981 8773-8781 VB denotes observed
T5982 8782-8784 IN denotes in
T5983 8785-8793 JJ denotes parental
T5984 8794-8800 NN denotes A20.2J
T5985 8801-8806 NN denotes cells
T5986 8807-8808 -LRB- denotes (
T5987 8808-8812 NN denotes Fig.
T5988 8813-8814 CD denotes 4
T5989 8814-8815 -RRB- denotes )
T5990 8817-8819 PRP denotes We
T5991 8820-8824 RB denotes also
T5992 8825-8832 VB denotes assayed
T5993 8833-8843 NN denotes activation
T5994 8844-8846 IN denotes of
T5995 8847-8850 DT denotes the
T5996 8851-8867 JJ denotes stress-activated
T5997 8868-8875 NN denotes protein
T5998 8876-8882 NN denotes kinase
T5999 8883-8886 NN denotes JNK
T6000 8887-8889 IN denotes in
T6001 8890-8898 NN denotes response
T6002 8899-8901 TO denotes to
T6003 8902-8906 NN denotes CD40
T6004 8907-8917 NN denotes engagement
T6005 8918-8919 -LRB- denotes (
T6006 8919-8923 NN denotes Fig.
T6007 8924-8925 CD denotes 4
T6008 8925-8926 -RRB- denotes )
T6009 8928-8941 JJ denotes CD40-mediated
T6010 8942-8945 NN denotes JNK
T6011 8946-8956 NN denotes activation
T6012 8957-8959 IN denotes in
T6013 8960-8974 JJ denotes HOIP-deficient
T6014 8975-8980 NN denotes cells
T6015 8981-8984 VB denotes was
T6016 8985-8993 VB denotes impaired
T6017 8994-8996 IN denotes as
T6018 8997-9005 VB denotes measured
T6019 9006-9008 IN denotes by
T6020 9009-9024 NN denotes phosphorylation
T6021 9025-9027 IN denotes of
T6022 9028-9034 NN denotes Thr183
T6023 9035-9038 CC denotes and
T6024 9039-9045 NN denotes Tyr185
T6025 9046-9048 IN denotes in
T6026 9049-9052 NN denotes JNK
T6027 9054-9066 JJ denotes CD40-induced
T6028 9067-9077 NN denotes activation
T6029 9078-9080 IN denotes of
T6030 9081-9086 NN denotes NF-κB
T6031 9087-9090 CC denotes and
T6032 9091-9094 NN denotes JNK
T6033 9095-9097 IN denotes in
T6034 9098-9116 JJ denotes HOIP-reconstituted
T6035 9117-9122 NN denotes cells
T6036 9123-9126 VB denotes was
T6037 9127-9133 JJ denotes normal
T6038 9133-9134 -COMMA- denotes ,
T6039 9135-9148 VB denotes demonstrating
T6040 9149-9153 IN denotes that
T6041 9154-9157 DT denotes the
T6042 9158-9165 NN denotes defects
T6043 9166-9174 VB denotes observed
T6044 9175-9177 IN denotes in
T6045 9178-9192 JJ denotes gene-deficient
T6046 9193-9198 NN denotes cells
T6047 9199-9203 VB denotes were
T6048 9204-9207 JJ denotes due
T6049 9208-9210 TO denotes to
T6050 9211-9214 DT denotes the
T6051 9215-9222 NN denotes absence
T6052 9223-9225 IN denotes of
T6053 9226-9230 NN denotes HOIP
T6054 9231-9241 NN denotes expression
T7483 8521-8528 NN denotes complex
T7484 10363-10367 IN denotes with
T7485 10368-10372 NN denotes CD40
T7486 10373-10376 DT denotes The
T7487 10377-10383 JJ denotes marked
T7488 10384-10391 NN denotes defects
T7489 10392-10394 IN denotes in
T7490 10395-10408 JJ denotes CD40-mediated
T7491 10409-10413 NN denotes cell
T7492 10414-10424 NN denotes activation
T7493 10425-10428 CC denotes and
T7494 10429-10438 NN denotes signaling
T7495 10439-10448 VB denotes displayed
T7496 10449-10451 IN denotes by
T7497 10452-10466 JJ denotes HOIP-deficient
T7498 10467-10472 NN denotes cells
T7499 10473-10482 VB denotes suggested
T7500 10483-10487 IN denotes that
T7501 10488-10492 NN denotes HOIP
T7502 10493-10501 VB denotes mediates
T7503 10502-10513 NN denotes recruitment
T7504 10514-10516 IN denotes of
T7505 10517-10525 JJ denotes critical
T7506 10526-10536 NN denotes components
T7507 10537-10539 IN denotes of
T7508 10540-10543 DT denotes the
T7509 10544-10548 NN denotes CD40
T7510 10549-10558 NN denotes signaling
T7511 10559-10568 NN denotes apparatus
T7512 10569-10571 TO denotes to
T7513 10572-10575 DT denotes the
T7514 10576-10584 NN denotes receptor
T7515 10586-10596 RB denotes Previously
T7516 10596-10597 -COMMA- denotes ,
T7517 10598-10600 PRP denotes we
T7518 10601-10613 VB denotes demonstrated
T7519 10614-10618 IN denotes that
T7520 10619-10623 NN denotes HOIP
T7521 10624-10626 VB denotes is
T7522 10627-10636 VB denotes recruited
T7523 10637-10639 TO denotes to
T7524 10640-10643 DT denotes the
T7525 10644-10648 NN denotes CD40
T7526 10649-10658 NN denotes signaling
T7527 10659-10666 NN denotes complex
T7528 10667-10669 IN denotes in
T7529 10670-10671 DT denotes a
T7530 10672-10687 JJ denotes TRAF2-dependent
T7531 10688-10694 NN denotes manner
T7532 10694-10695 -COMMA- denotes ,
T7533 10696-10706 VB denotes suggesting
T7534 10707-10711 IN denotes that
T7535 10712-10716 NN denotes HOIP
T7536 10717-10726 NN denotes functions
T7537 10727-10737 JJ denotes downstream
T7538 10738-10740 IN denotes of
T7539 10741-10746 NN denotes TRAF2
T7540 10747-10748 -LRB- denotes [
T7541 10748-10749 CD denotes 6
T7542 10749-10750 -RRB- denotes ]
T7543 10752-10759 NN denotes Studies
T7544 10760-10762 IN denotes by
T7545 10763-10769 NN denotes others
T7546 10770-10777 VB denotes suggest
T7547 10778-10782 IN denotes that
T7548 10783-10786 DT denotes the
T7549 10787-10803 JJ denotes TRAF2-associated
T7550 10804-10812 NN denotes proteins
T7551 10813-10818 NN denotes cIAP1
T7552 10819-10822 CC denotes and
T7553 10823-10828 NN denotes cIAP2
T7554 10829-10833 VB denotes play
T7555 10834-10835 DT denotes a
T7556 10836-10840 NN denotes role
T7557 10841-10843 IN denotes in
T7558 10844-10847 DT denotes the
T7559 10848-10859 NN denotes recruitment
T7560 10860-10862 IN denotes of
T7561 10863-10867 NN denotes HOIP
T7562 10868-10870 TO denotes to
T7563 10871-10876 NN denotes TNFR1
T7564 10877-10880 CC denotes and
T7565 10881-10885 NN denotes CD40
T7566 10886-10887 -LRB- denotes [
T7567 10887-10889 CD denotes 14
T7568 10889-10890 -RRB- denotes ]
T7569 10892-10901 RB denotes Therefore
T7570 10901-10902 -COMMA- denotes ,
T7571 10903-10905 PRP denotes we
T7572 10906-10916 VB denotes determined
T7573 10917-10924 IN denotes whether
T7574 10925-10928 DT denotes the
T7575 10929-10940 NN denotes association
T7576 10941-10943 IN denotes of
T7577 10944-10948 NN denotes HOIP
T7578 10949-10953 IN denotes with
T7579 10954-10958 NN denotes CD40
T7580 10959-10961 IN denotes in
T7581 10962-10968 NN denotes A20.2J
T7582 10969-10974 NN denotes cells
T7583 10975-10978 VB denotes was
T7584 10979-10986 VB denotes altered
T7585 10987-10989 IN denotes by
T7586 10990-10999 NN denotes treatment
T7587 11000-11002 IN denotes of
T7588 11003-11008 NN denotes cells
T7589 11009-11013 IN denotes with
T7590 11014-11016 DT denotes an
T7591 11017-11026 NN denotes inhibitor
T7592 11027-11029 IN denotes of
T7593 11030-11034 NN denotes cIAP
T7594 11035-11043 NN denotes activity
T7595 11043-11044 -COMMA- denotes ,
T7596 11045-11046 DT denotes a
T7597 11047-11065 JJ denotes membrane-permeable
T7598 11066-11073 NN denotes peptide
T7599 11074-11081 VB denotes derived
T7600 11082-11086 IN denotes from
T7601 11087-11090 DT denotes the
T7602 11091-11100 NN denotes apoptosis
T7603 11101-11110 NN denotes regulator
T7604 11111-11115 NN denotes SMAC
T7605 11116-11117 -LRB- denotes [
T7606 11117-11119 CD denotes 15
T7607 11119-11120 -RRB- denotes ]
T7608 11122-11124 PRP denotes We
T7609 11125-11130 VB denotes found
T7610 11131-11135 IN denotes that
T7611 11136-11148 NN denotes pretreatment
T7612 11149-11151 IN denotes of
T7613 11152-11157 NN denotes cells
T7614 11158-11162 IN denotes with
T7615 11163-11166 DT denotes the
T7616 11167-11171 NN denotes SMAC
T7617 11172-11179 NN denotes peptide
T7618 11180-11192 RB denotes dramatically
T7619 11193-11200 VB denotes reduced
T7620 11201-11204 DT denotes the
T7621 11205-11211 NN denotes amount
T7622 11212-11214 IN denotes of
T7623 11215-11220 NN denotes cIAP1
T7624 11221-11231 VB denotes associated
T7625 11232-11236 IN denotes with
T7626 11237-11240 DT denotes the
T7627 11241-11245 NN denotes CD40
T7628 11246-11255 NN denotes signaling
T7629 11256-11263 NN denotes complex
T7630 11264-11266 IN denotes in
T7631 11267-11272 NN denotes cells
T7632 11273-11283 VB denotes stimulated
T7633 11284-11288 IN denotes with
T7634 11289-11298 JJ denotes anti-CD40
T7635 11299-11314 JJ denotes antibody-coated
T7636 11315-11320 NN denotes beads
T7637 11321-11322 -LRB- denotes (
T7638 11322-11326 NNP denotes Fig.
T7639 11327-11329 NN denotes 5A
T7640 11329-11330 -RRB- denotes )
T7641 11332-11336 NN denotes SMAC
T7642 11337-11344 NN denotes peptide
T7643 11345-11354 NN denotes treatment
T7644 11355-11359 RB denotes also
T7645 11360-11368 VB denotes resulted
T7646 11369-11371 IN denotes in
T7647 11372-11373 DT denotes a
T7648 11374-11380 JJ denotes slight
T7649 11381-11384 CC denotes but
T7650 11385-11397 JJ denotes reproducible
T7651 11398-11406 NN denotes decrease
T7652 11407-11409 IN denotes in
T7653 11410-11413 DT denotes the
T7654 11414-11420 NN denotes amount
T7655 11421-11423 IN denotes of
T7656 11424-11427 DT denotes the
T7657 11428-11433 JJ denotes major
T7658 11434-11438 NN denotes HOIP
T7659 11439-11443 NN denotes form
T7660 11444-11453 VB denotes recovered
T7661 11454-11456 IN denotes by
T7662 11457-11461 NN denotes CD40
T7663 11462-11481 NN denotes immunoprecipitation
T7664 11481-11482 -COMMA- denotes ,
T7665 11483-11488 IN denotes along
T7666 11489-11493 IN denotes with
T7667 11494-11496 DT denotes an
T7668 11497-11505 JJ denotes apparent
T7669 11506-11514 NN denotes increase
T7670 11515-11517 IN denotes in
T7671 11518-11524 JJ denotes higher
T7672 11525-11534 JJ denotes molecular
T7673 11535-11541 NN denotes weight
T7674 11542-11549 NN denotes species
T7675 11550-11560 VB denotes recognized
T7676 11561-11563 IN denotes by
T7677 11564-11573 JJ denotes anti-HOIP
T7678 11574-11582 NN denotes antibody
T7679 11584-11586 IN denotes In
T7680 11587-11595 NN denotes contrast
T7681 11595-11596 -COMMA- denotes ,
T7682 11597-11606 NN denotes treatment
T7683 11607-11611 IN denotes with
T7684 11612-11615 DT denotes the
T7685 11616-11620 NN denotes SMAC
T7686 11621-11628 NN denotes peptide
T7687 11629-11632 VB denotes did
T7688 11633-11636 RB denotes not
T7689 11637-11642 VB denotes alter
T7690 11643-11646 DT denotes the
T7691 11647-11653 NN denotes amount
T7692 11654-11656 CC denotes or
T7693 11657-11666 JJ denotes molecular
T7694 11667-11673 NN denotes weight
T7695 11674-11676 IN denotes of
T7696 11677-11681 NN denotes HOIP
T7697 11682-11689 JJ denotes present
T7698 11690-11692 IN denotes in
T7699 11693-11697 NN denotes cell
T7700 11698-11705 NN denotes lysates
T7701 11707-11712 DT denotes These
T7702 11713-11717 NN denotes data
T7703 11718-11725 VB denotes suggest
T7704 11726-11730 IN denotes that
T7705 11731-11735 NN denotes SMAC
T7706 11736-11743 NN denotes peptide
T7707 11744-11753 NN denotes treatment
T7708 11754-11766 RB denotes specifically
T7709 11767-11773 VB denotes alters
T7710 11774-11777 DT denotes the
T7711 11778-11793 NN denotes characteristics
T7712 11794-11796 IN denotes of
T7713 11797-11812 JJ denotes CD40-associated
T7714 11813-11817 NN denotes HOIP
T7715 11818-11824 RB denotes rather
T7716 11825-11829 IN denotes than
T7717 11830-11833 DT denotes the
T7718 11834-11840 JJ denotes entire
T7719 11841-11849 JJ denotes cellular
T7720 11850-11854 NN denotes pool
T7721 11855-11857 IN denotes of
T7722 11858-11862 DT denotes this
T7723 11863-11870 NN denotes protein
T7724 11872-11880 RB denotes Together
T7725 11880-11881 -COMMA- denotes ,
T7726 11882-11887 DT denotes these
T7727 11888-11895 NN denotes results
T7728 11896-11903 VB denotes support
T7729 11904-11907 DT denotes the
T7730 11908-11912 NN denotes idea
T7731 11913-11917 IN denotes that
T7732 11918-11921 DT denotes the
T7733 11922-11926 NN denotes cIAP
T7734 11927-11935 NN denotes proteins
T7735 11936-11945 VB denotes influence
T7736 11946-11949 DT denotes the
T7737 11950-11961 NN denotes recruitment
T7738 11962-11965 CC denotes and
T7739 11966-11984 JJ denotes post-translational
T7740 11985-11997 NN denotes modification
T7741 11998-12003 NN denotes state
T7742 12004-12006 IN denotes of
T7743 12007-12022 JJ denotes CD40-associated
T7744 12023-12027 NN denotes HOIP
T7745 14482-14484 TO denotes To
T7746 14485-14489 VB denotes test
T7747 14490-14493 DT denotes the
T7748 14494-14505 NN denotes possibility
T7749 14506-14510 IN denotes that
T7750 14511-14515 NN denotes HOIP
T7751 14516-14518 VB denotes is
T7752 14519-14530 JJ denotes responsible
T7753 14531-14534 IN denotes for
T7754 14535-14538 DT denotes the
T7755 14539-14550 NN denotes recruitment
T7756 14551-14553 IN denotes of
T7757 14554-14559 JJ denotes other
T7758 14560-14568 JJ denotes critical
T7759 14569-14578 NN denotes signaling
T7760 14579-14587 NN denotes proteins
T7761 14588-14590 TO denotes to
T7762 14591-14595 NN denotes CD40
T7763 14595-14596 -COMMA- denotes ,
T7764 14597-14599 PRP denotes we
T7765 14600-14618 VB denotes immunoprecipitated
T7766 14619-14623 NN denotes CD40
T7767 14624-14633 NN denotes signaling
T7768 14634-14643 NN denotes complexes
T7769 14644-14648 IN denotes from
T7770 14649-14663 JJ denotes HOIP-deficient
T7771 14664-14667 CC denotes and
T7772 14668-14686 JJ denotes HOIP-reconstituted
T7773 14687-14692 NN denotes cells
T7774 14693-14694 -LRB- denotes (
T7775 14694-14698 NN denotes Fig.
T7776 14699-14701 NN denotes 5B
T7777 14701-14702 -RRB- denotes )
T7778 14704-14706 IN denotes As
T7779 14707-14716 VB denotes described
T7780 14717-14727 RB denotes previously
T7781 14727-14728 -COMMA- denotes ,
T7782 14729-14733 NN denotes CD40
T7783 14734-14743 NN denotes signaling
T7784 14744-14753 NN denotes complexes
T7785 14754-14772 VB denotes immunoprecipitated
T7786 14773-14777 IN denotes from
T7787 14778-14784 NN denotes A20.2J
T7788 14785-14790 NN denotes cells
T7789 14791-14800 VB denotes contained
T7790 14801-14805 NN denotes HOIP
T7791 14806-14808 RB denotes as
T7792 14809-14813 RB denotes well
T7793 14814-14816 IN denotes as
T7794 14817-14822 NN denotes TRAF2
T7795 14822-14823 -COMMA- denotes ,
T7796 14824-14829 NN denotes TRAF3
T7797 14829-14830 -COMMA- denotes ,
T7798 14831-14837 NN denotes IKKα/β
T7799 14837-14838 -COMMA- denotes ,
T7800 14839-14842 CC denotes and
T7801 14843-14847 NNP denotes IKKγ
T7802 14848-14849 -LRB- denotes [
T7803 14849-14850 CD denotes 6
T7804 14850-14851 -RRB- denotes ]
T7805 14853-14856 DT denotes The
T7806 14857-14864 NN denotes amounts
T7807 14865-14867 IN denotes of
T7808 14868-14873 NN denotes TRAF2
T7809 14873-14874 -COMMA- denotes ,
T7810 14875-14880 NN denotes TRAF3
T7811 14880-14881 -COMMA- denotes ,
T7812 14882-14885 CC denotes and
T7813 14886-14891 NN denotes cIAP1
T7814 14892-14894 IN denotes in
T7815 14895-14899 NN denotes CD40
T7816 14900-14918 NN denotes immunoprecipitates
T7817 14919-14923 IN denotes from
T7818 14924-14938 JJ denotes HOIP-deficient
T7819 14939-14944 NN denotes cells
T7820 14945-14949 VB denotes were
T7821 14950-14957 JJ denotes similar
T7822 14958-14960 TO denotes to
T7823 14961-14966 DT denotes those
T7824 14967-14971 IN denotes from
T7825 14972-14980 JJ denotes parental
T7826 14981-14987 NN denotes A20.2J
T7827 14988-14993 NN denotes cells
T7828 14993-14994 -COMMA- denotes ,
T7829 14995-15005 VB denotes indicating
T7830 15006-15010 IN denotes that
T7831 15011-15015 NN denotes HOIP
T7832 15016-15018 VB denotes is
T7833 15019-15022 RB denotes not
T7834 15023-15031 VB denotes required
T7835 15032-15035 IN denotes for
T7836 15036-15039 DT denotes the
T7837 15040-15051 NN denotes association
T7838 15052-15054 IN denotes of
T7839 15055-15060 DT denotes these
T7840 15061-15069 NN denotes proteins
T7841 15070-15074 IN denotes with
T7842 15075-15079 NN denotes CD40
T7843 15081-15083 IN denotes In
T7844 15084-15092 NN denotes contrast
T7845 15092-15093 -COMMA- denotes ,
T7846 15094-15100 NN denotes IKKα/β
T7847 15101-15104 CC denotes and
T7848 15105-15109 NN denotes IKKγ
T7849 15110-15114 VB denotes were
T7850 15115-15118 RB denotes not
T7851 15119-15129 JJ denotes detectable
T7852 15130-15132 IN denotes in
T7853 15133-15137 NN denotes CD40
T7854 15138-15156 NN denotes immunoprecipitates
T7855 15157-15166 VB denotes recovered
T7856 15167-15171 IN denotes from
T7857 15172-15186 JJ denotes HOIP-deficient
T7858 15187-15192 NN denotes cells
T7859 15193-15194 -LRB- denotes (
T7860 15194-15198 NN denotes Fig.
T7861 15199-15201 NN denotes 5B
T7862 15201-15202 -COMMA- denotes ,
T7863 15202-15203 NN denotes C
T7864 15203-15204 -RRB- denotes )
T7865 15206-15209 DT denotes The
T7866 15210-15217 NN denotes amounts
T7867 15218-15220 IN denotes of
T7868 15221-15227 NN denotes IKKα/β
T7869 15228-15231 CC denotes and
T7870 15232-15236 NN denotes IKKγ
T7871 15237-15239 IN denotes in
T7872 15240-15244 NN denotes CD40
T7873 15245-15263 NN denotes immunoprecipitates
T7874 15264-15268 IN denotes from
T7875 15269-15287 JJ denotes HOIP-reconstituted
T7876 15288-15293 NN denotes cells
T7877 15294-15298 VB denotes were
T7878 15299-15306 JJ denotes similar
T7879 15307-15309 TO denotes to
T7880 15310-15315 DT denotes those
T7881 15316-15324 VB denotes detected
T7882 15325-15327 IN denotes in
T7883 15328-15335 NN denotes samples
T7884 15336-15344 VB denotes prepared
T7885 15345-15349 IN denotes from
T7886 15350-15358 JJ denotes parental
T7887 15359-15364 NN denotes cells
T7888 15364-15365 -COMMA- denotes ,
T7889 15366-15379 VB denotes demonstrating
T7890 15380-15384 IN denotes that
T7891 15385-15388 DT denotes the
T7892 15389-15396 NN denotes defects
T7893 15397-15399 IN denotes in
T7894 15400-15403 NN denotes IKK
T7895 15404-15415 NN denotes recruitment
T7896 15416-15418 PRP denotes we
T7897 15419-15427 VB denotes observed
T7898 15428-15432 VB denotes were
T7899 15433-15436 JJ denotes due
T7900 15437-15449 RB denotes specifically
T7901 15450-15452 TO denotes to
T7902 15453-15456 DT denotes the
T7903 15457-15464 NN denotes absence
T7904 15465-15467 IN denotes of
T7905 15468-15472 NN denotes HOIP
T7906 15473-15483 NN denotes expression
T7907 15485-15490 DT denotes These
T7908 15491-15495 NN denotes data
T7909 15496-15504 VB denotes indicate
T7910 15505-15509 IN denotes that
T7911 15510-15514 NN denotes HOIP
T7912 15515-15517 VB denotes is
T7913 15518-15526 VB denotes required
T7914 15527-15530 IN denotes for
T7915 15531-15542 NN denotes recruitment
T7916 15543-15545 IN denotes of
T7917 15546-15549 DT denotes the
T7918 15550-15566 JJ denotes NF-κB-activating
T7919 15567-15574 NN denotes complex
T7920 15575-15577 TO denotes to
T7921 15578-15581 DT denotes the
T7922 15582-15586 NN denotes CD40
T7923 15587-15596 NN denotes signaling
T7924 15597-15604 NN denotes complex
T7925 15606-15608 IN denotes As
T7926 15609-15614 VB denotes shown
T7927 15615-15619 RB denotes here
T7928 15620-15623 CC denotes and
T7929 15624-15626 IN denotes in
T7930 15627-15630 PRP-DOLLAR- denotes our
T7931 15631-15639 JJ denotes previous
T7932 15640-15645 NN denotes study
T7933 15646-15647 -LRB- denotes [
T7934 15647-15648 CD denotes 6
T7935 15648-15649 -RRB- denotes ]
T7936 15649-15650 -COMMA- denotes ,
T7937 15651-15655 NN denotes IKKγ
T7938 15656-15663 JJ denotes present
T7939 15664-15666 IN denotes in
T7940 15667-15671 NN denotes CD40
T7941 15672-15690 NN denotes immunoprecipitates
T7942 15691-15698 VB denotes appears
T7943 15699-15701 TO denotes to
T7944 15702-15706 VB denotes have
T7945 15707-15708 DT denotes a
T7946 15709-15715 JJ denotes higher
T7947 15716-15725 JJ denotes molecular
T7948 15726-15732 NN denotes weight
T7949 15733-15737 IN denotes than
T7950 15738-15742 DT denotes that
T7951 15743-15748 VB denotes found
T7952 15749-15751 IN denotes in
T7953 15752-15756 NN denotes cell
T7954 15757-15764 NN denotes lysates
T7955 15766-15768 TO denotes To
T7956 15769-15776 VB denotes confirm
T7957 15777-15781 IN denotes that
T7958 15782-15786 DT denotes this
T7959 15787-15793 JJ denotes higher
T7960 15794-15803 JJ denotes molecular
T7961 15804-15810 NN denotes weight
T7962 15811-15818 NN denotes species
T7963 15819-15822 VB denotes was
T7964 15823-15829 RB denotes indeed
T7965 15830-15834 NN denotes IKKγ
T7966 15834-15835 -COMMA- denotes ,
T7967 15836-15838 PRP denotes we
T7968 15839-15848 VB denotes generated
T7969 15849-15855 NN denotes A20.2J
T7970 15856-15860 NN denotes cell
T7971 15861-15866 NN denotes lines
T7972 15867-15877 VB denotes containing
T7973 15878-15879 DT denotes a
T7974 15880-15886 RB denotes stably
T7975 15887-15897 VB denotes integrated
T7976 15898-15908 JJ denotes retroviral
T7977 15909-15915 NN denotes vector
T7978 15916-15920 WDT denotes that
T7979 15921-15928 VB denotes encoded
T7980 15929-15931 DT denotes an
T7981 15932-15946 JJ denotes epitope-tagged
T7982 15947-15954 NN denotes version
T7983 15955-15957 IN denotes of
T7984 15958-15963 NN denotes mouse
T7985 15964-15968 NN denotes IKKγ
T7986 15970-15974 NN denotes Cell
T7987 15975-15982 NN denotes lysates
T7988 15983-15986 CC denotes and
T7989 15987-15991 NN denotes CD40
T7990 15992-16010 NN denotes immunoprecipitates
T7991 16011-16019 VB denotes prepared
T7992 16020-16024 IN denotes from
T7993 16025-16030 DT denotes these
T7994 16031-16036 NN denotes cells
T7995 16037-16041 VB denotes were
T7996 16042-16050 VB denotes analyzed
T7997 16051-16053 IN denotes by
T7998 16054-16061 JJ denotes Western
T7999 16062-16070 NN denotes blotting
T8000 16071-16074 IN denotes for
T8001 16075-16078 DT denotes the
T8002 16079-16086 NN denotes epitope
T8003 16087-16090 NN denotes tag
T8004 16091-16092 -LRB- denotes (
T8005 16092-16096 NN denotes Fig.
T8006 16097-16099 NN denotes 5C
T8007 16099-16100 -RRB- denotes )
T8008 16102-16106 DT denotes This
T8009 16107-16115 NN denotes analysis
T8010 16116-16124 VB denotes produced
T8011 16125-16126 DT denotes a
T8012 16127-16134 NN denotes pattern
T8013 16135-16137 IN denotes of
T8014 16138-16143 NN denotes bands
T8015 16144-16148 WDT denotes that
T8016 16149-16152 VB denotes was
T8017 16153-16164 RB denotes essentially
T8018 16165-16168 DT denotes the
T8019 16169-16173 JJ denotes same
T8020 16174-16176 IN denotes as
T8021 16177-16181 DT denotes that
T8022 16182-16190 VB denotes obtained
T8023 16191-16196 VB denotes using
T8024 16197-16199 DT denotes an
T8025 16200-16208 NN denotes antibody
T8026 16209-16217 JJ denotes specific
T8027 16218-16221 IN denotes for
T8028 16222-16228 JJ denotes native
T8029 16229-16233 NN denotes IKKγ
T8030 16234-16235 -LRB- denotes (
T8031 16235-16242 NN denotes compare
T8032 16243-16249 NN denotes panels
T8033 16250-16251 NN denotes B
T8034 16252-16255 CC denotes and
T8035 16256-16257 NN denotes C
T8036 16258-16260 IN denotes in
T8037 16261-16265 NNP denotes Fig.
T8038 16266-16267 CD denotes 5
T8039 16267-16268 -RRB- denotes )
T8040 16270-16278 RB denotes Moreover
T8041 16278-16279 -COMMA- denotes ,
T8042 16280-16288 NN denotes analysis
T8043 16289-16291 IN denotes of
T8044 16292-16297 NN denotes cells
T8045 16298-16308 VB denotes expressing
T8046 16309-16316 NN denotes epitope
T8047 16317-16328 NN denotes tagged-IKKγ
T8048 16329-16338 VB denotes confirmed
T8049 16339-16343 IN denotes that
T8050 16344-16355 NN denotes recruitment
T8051 16356-16358 IN denotes of
T8052 16359-16363 NN denotes IKKγ
T8053 16364-16366 TO denotes to
T8054 16367-16371 NN denotes CD40
T8055 16372-16380 VB denotes requires
T8056 16381-16385 NN denotes HOIP
T8057 16387-16389 TO denotes To
T8058 16390-16399 VB denotes determine
T8059 16400-16404 WP denotes what
T8060 16405-16408 VB denotes was
T8061 16409-16420 JJ denotes responsible
T8062 16421-16424 IN denotes for
T8063 16425-16428 DT denotes the
T8064 16429-16438 VB denotes increased
T8065 16439-16448 JJ denotes molecular
T8066 16449-16455 NN denotes weight
T8067 16456-16458 IN denotes of
T8068 16459-16463 NN denotes IKKγ
T8069 16464-16466 IN denotes in
T8070 16467-16471 NN denotes CD40
T8071 16472-16490 NN denotes immunoprecipitates
T8072 16490-16491 -COMMA- denotes ,
T8073 16492-16495 DT denotes the
T8074 16496-16503 NN denotes protein
T8075 16504-16511 NN denotes samples
T8076 16512-16516 VB denotes were
T8077 16517-16526 VB denotes incubated
T8078 16527-16531 IN denotes with
T8079 16532-16538 NN denotes lambda
T8080 16539-16550 NN denotes phosphatase
T8081 16550-16551 -COMMA- denotes ,
T8082 16552-16557 WDT denotes which
T8083 16558-16565 VB denotes removes
T8084 16566-16576 NN denotes phosphates
T8085 16577-16585 VB denotes attached
T8086 16586-16588 TO denotes to
T8087 16589-16597 NN denotes tyrosine
T8088 16597-16598 -COMMA- denotes ,
T8089 16599-16608 NN denotes threonine
T8090 16608-16609 -COMMA- denotes ,
T8091 16610-16612 CC denotes or
T8092 16613-16619 NN denotes serine
T8093 16620-16628 NN denotes residues
T8094 16629-16631 IN denotes in
T8095 16632-16640 NN denotes proteins
T8096 16642-16646 DT denotes This
T8097 16647-16656 NN denotes treatment
T8098 16657-16664 VB denotes reduced
T8099 16665-16669 JJ denotes much
T8100 16670-16672 IN denotes of
T8101 16673-16688 JJ denotes CD40-associated
T8102 16689-16693 NN denotes IKKγ
T8103 16694-16696 TO denotes to
T8104 16697-16699 DT denotes an
T8105 16700-16708 JJ denotes apparent
T8106 16709-16718 JJ denotes molecular
T8107 16719-16725 NN denotes weight
T8108 16726-16733 JJ denotes similar
T8109 16734-16736 TO denotes to
T8110 16737-16741 DT denotes that
T8111 16742-16744 IN denotes of
T8112 16745-16748 DT denotes the
T8113 16749-16756 NN denotes protein
T8114 16757-16759 IN denotes in
T8115 16760-16764 NN denotes cell
T8116 16765-16772 NN denotes lysates
T8117 16773-16774 -LRB- denotes (
T8118 16774-16778 NNP denotes Fig.
T8119 16779-16781 NN denotes 5C
T8120 16781-16782 -RRB- denotes )
T8121 16784-16791 RB denotes However
T8122 16791-16792 -COMMA- denotes ,
T8123 16793-16795 IN denotes at
T8124 16796-16801 JJ denotes least
T8125 16802-16805 CD denotes one
T8126 16806-16810 NN denotes band
T8127 16811-16813 IN denotes of
T8128 16814-16827 RB denotes significantly
T8129 16828-16834 JJ denotes higher
T8130 16835-16844 JJ denotes molecular
T8131 16845-16851 NN denotes weight
T8132 16852-16860 VB denotes remained
T8133 16861-16866 IN denotes after
T8134 16867-16878 NN denotes phosphatase
T8135 16879-16888 NN denotes treatment
T8136 16888-16889 -COMMA- denotes ,
T8137 16890-16900 VB denotes suggesting
T8138 16901-16905 IN denotes that
T8139 16906-16921 JJ denotes CD40-associated
T8140 16922-16926 NN denotes IKKγ
T8141 16927-16929 VB denotes is
T8142 16930-16937 JJ denotes subject
T8143 16938-16940 TO denotes to
T8144 16941-16943 IN denotes at
T8145 16944-16949 JJ denotes least
T8146 16950-16953 CD denotes one
T8147 16954-16959 JJ denotes other
T8148 16960-16972 NN denotes modification
T8149 16973-16975 IN denotes in
T8150 16976-16984 NN denotes addition
T8151 16985-16987 TO denotes to
T8152 16988-17003 NN denotes phosphorylation
T8153 17005-17012 RB denotes Overall
T8154 17012-17013 -COMMA- denotes ,
T8155 17014-17019 DT denotes these
T8156 17020-17024 NN denotes data
T8157 17025-17036 VB denotes demonstrate
T8158 17037-17041 IN denotes that
T8159 17042-17046 NN denotes HOIP
T8160 17047-17049 VB denotes is
T8161 17050-17058 VB denotes required
T8162 17059-17062 IN denotes for
T8163 17063-17066 DT denotes the
T8164 17067-17078 NN denotes association
T8165 17079-17081 IN denotes of
T8166 17082-17086 NN denotes IKKγ
T8167 17087-17091 IN denotes with
T8168 17092-17096 NN denotes CD40
T8169 17096-17097 -COMMA- denotes ,
T8170 17098-17101 CC denotes and
T8171 17102-17109 VB denotes suggest
T8172 17110-17114 IN denotes that
T8173 17115-17119 DT denotes this
T8174 17120-17125 NN denotes event
T8175 17126-17128 VB denotes is
T8176 17129-17136 VB denotes coupled
T8177 17137-17139 TO denotes to
T8178 17140-17158 JJ denotes post-translational
T8179 17159-17172 NN denotes modifications
T8180 17173-17175 IN denotes of
T8181 17176-17180 NN denotes IKKγ
T15027 2125-2129 NN denotes HOIP
T15028 2130-2134 NN denotes gene
T15029 2135-2144 NN denotes targeting
T15030 2146-2147 -LRB- denotes (
T15031 2147-2148 NN denotes A
T15032 2148-2149 -RRB- denotes )
T15033 2150-2157 NN denotes Regions
T15034 2158-2160 IN denotes of
T15035 2161-2166 NN denotes Rnf31
T15036 2167-2171 NN denotes gene
T15037 2172-2180 NN denotes sequence
T15038 2181-2182 -LRB- denotes (
T15039 2182-2188 NN denotes bottom
T15040 2188-2189 -RRB- denotes )
T15041 2190-2194 VB denotes used
T15042 2195-2197 IN denotes in
T15043 2198-2201 DT denotes the
T15044 2202-2206 NN denotes HOIP
T15045 2207-2211 NN denotes gene
T15046 2212-2221 NN denotes targeting
T15047 2222-2228 NN denotes vector
T15048 2229-2230 -LRB- denotes (
T15049 2230-2233 NN denotes top
T15050 2233-2234 -RRB- denotes )
T15051 2235-2238 VB denotes are
T15052 2239-2244 VB denotes shown
T15053 2246-2252 NN denotes Arrows
T15054 2253-2254 -LRB- denotes (
T15055 2254-2255 NN denotes F
T15056 2256-2259 CC denotes and
T15057 2260-2261 NN denotes R
T15058 2261-2262 -RRB- denotes )
T15059 2263-2271 VB denotes indicate
T15060 2272-2283 JJ denotes approximate
T15061 2284-2293 NN denotes positions
T15062 2294-2296 IN denotes of
T15063 2297-2306 NN denotes sequences
T15064 2307-2317 JJ denotes homologous
T15065 2318-2320 TO denotes to
T15066 2321-2337 NN denotes oligonucleotides
T15067 2338-2342 VB denotes used
T15068 2343-2346 IN denotes for
T15069 2347-2359 JJ denotes PCR-mediated
T15070 2360-2369 NN denotes detection
T15071 2370-2372 IN denotes of
T15072 2373-2383 JJ denotes homologous
T15073 2384-2397 NN denotes recombination
T15074 2399-2409 JJ denotes Homologous
T15075 2410-2423 NN denotes recombination
T15076 2424-2426 IN denotes of
T15077 2427-2430 DT denotes the
T15078 2431-2437 NN denotes vector
T15079 2438-2442 IN denotes with
T15080 2443-2448 NN denotes Rnf31
T15081 2449-2457 VB denotes resulted
T15082 2458-2460 IN denotes in
T15083 2461-2462 DT denotes a
T15084 2463-2468 JJ denotes small
T15085 2469-2480 JJ denotes chromosomal
T15086 2481-2489 NN denotes deletion
T15087 2490-2493 CC denotes and
T15088 2494-2497 DT denotes the
T15089 2498-2506 JJ denotes in-frame
T15090 2507-2516 NN denotes insertion
T15091 2517-2519 IN denotes of
T15092 2520-2528 NN denotes neomycin
T15093 2529-2547 NN denotes phosphotransferase
T15094 2548-2549 -LRB- denotes (
T15095 2549-2553 NN denotes NeoR
T15096 2553-2554 -RRB- denotes )
T15097 2555-2559 IN denotes into
T15098 2560-2563 DT denotes the
T15099 2564-2578 JJ denotes amino-terminal
T15100 2579-2583 NN denotes HOIP
T15101 2584-2590 VB denotes coding
T15102 2591-2599 NN denotes sequence
T15103 2601-2602 DT denotes A
T15104 2603-2613 NN denotes diphtheria
T15105 2614-2619 NN denotes toxin
T15106 2620-2621 -LRB- denotes (
T15107 2621-2623 NN denotes DT
T15108 2623-2624 -RRB- denotes )
T15109 2625-2633 NN denotes cassette
T15110 2634-2636 IN denotes in
T15111 2637-2640 DT denotes the
T15112 2641-2647 NN denotes vector
T15113 2648-2659 VB denotes facilitates
T15114 2660-2663 DT denotes the
T15115 2664-2672 JJ denotes negative
T15116 2673-2682 NN denotes selection
T15117 2683-2685 IN denotes of
T15118 2686-2691 NN denotes cells
T15119 2692-2694 IN denotes in
T15120 2695-2700 WDT denotes which
T15121 2701-2707 VB denotes random
T15122 2708-2719 JJ denotes chromosomal
T15123 2720-2729 NN denotes insertion
T15124 2730-2732 IN denotes of
T15125 2733-2736 DT denotes the
T15126 2737-2743 NN denotes vector
T15127 2744-2749 VB denotes takes
T15128 2750-2755 NN denotes place
T15129 2757-2761 NN denotes LoxP
T15130 2762-2771 NN denotes sequences
T15131 2772-2777 VB denotes allow
T15132 2778-2781 DT denotes the
T15133 2782-2794 JJ denotes Cre-mediated
T15134 2795-2803 NN denotes deletion
T15135 2804-2806 IN denotes of
T15136 2807-2810 DT denotes the
T15137 2811-2815 NN denotes NeoR
T15138 2816-2822 NN denotes coding
T15139 2823-2831 NN denotes sequence
T15140 2833-2836 DT denotes The
T15141 2837-2843 NN denotes SV40pA
T15142 2844-2852 NN denotes sequence
T15143 2853-2858 VB denotes helps
T15144 2859-2861 TO denotes to
T15145 2862-2868 VB denotes ensure
T15146 2869-2879 NN denotes disruption
T15147 2880-2882 IN denotes of
T15148 2883-2887 NN denotes gene
T15149 2888-2898 NN denotes expression
T15150 2899-2904 IN denotes after
T15151 2905-2913 NN denotes deletion
T15152 2914-2916 IN denotes of
T15153 2917-2920 DT denotes the
T15154 2921-2925 NN denotes NeoR
T15155 2926-2935 NN denotes sequence.
T15156 2936-2937 -LRB- denotes (
T15157 2937-2938 NN denotes B
T15158 2938-2939 -RRB- denotes )
T15159 2940-2949 NN denotes Anti-HOIP
T15160 2950-2953 CC denotes and
T15161 2954-2963 JJ denotes anti-FLAG
T15162 2964-2971 JJ denotes Western
T15163 2972-2977 NN denotes blots
T15164 2978-2980 IN denotes of
T15165 2981-2985 NN denotes cell
T15166 2986-2993 NN denotes lysates
T15167 2994-2998 IN denotes from
T15168 2999-3005 NN denotes A20.2J
T15169 3006-3011 NN denotes cells
T15170 3012-3015 CC denotes and
T15171 3016-3030 JJ denotes HOIP-deficient
T15172 3031-3032 -LRB- denotes (
T15173 3032-3039 JJ denotes HOIP-/-
T15174 3039-3040 -RRB- denotes )
T15175 3041-3047 NN denotes clones
T15176 3049-3050 DT denotes A
T15177 3051-3058 JJ denotes partial
T15178 3059-3067 NN denotes decrease
T15179 3068-3070 IN denotes in
T15180 3071-3075 NN denotes HOIP
T15181 3076-3083 NN denotes protein
T15182 3084-3094 NN denotes expression
T15183 3095-3097 VB denotes is
T15184 3098-3105 JJ denotes evident
T15185 3106-3108 IN denotes in
T15186 3109-3114 NN denotes cells
T15187 3115-3124 VB denotes following
T15188 3125-3135 NN denotes disruption
T15189 3136-3138 IN denotes of
T15190 3139-3142 CD denotes one
T15191 3143-3147 NN denotes copy
T15192 3148-3150 IN denotes of
T15193 3151-3156 NN denotes Rnf31
T15194 3157-3158 -LRB- denotes (
T15195 3158-3165 NN denotes HOIP−/+
T15196 3165-3166 -RRB- denotes )
T15197 3168-3172 NN denotes HOIP
T15198 3173-3183 NN denotes expression
T15199 3184-3186 IN denotes in
T15200 3187-3193 NN denotes clones
T15201 3194-3207 VB denotes reconstituted
T15202 3208-3212 IN denotes with
T15203 3213-3215 DT denotes an
T15204 3216-3221 JJ denotes empty
T15205 3222-3232 JJ denotes retroviral
T15206 3233-3239 NN denotes vector
T15207 3240-3241 -LRB- denotes (
T15208 3241-3245 NN denotes pMIP
T15209 3245-3246 -RRB- denotes )
T15210 3247-3249 CC denotes or
T15211 3250-3251 DT denotes a
T15212 3252-3262 JJ denotes retroviral
T15213 3263-3269 NN denotes vector
T15214 3270-3278 VB denotes encoding
T15215 3279-3290 JJ denotes FLAG-tagged
T15216 3291-3295 NN denotes HOIP
T15217 3296-3298 VB denotes is
T15218 3299-3303 RB denotes also
T15219 3304-3309 VB denotes shown
T15220 3311-3322 JJ denotes Approximate
T15221 3323-3332 JJ denotes molecular
T15222 3333-3339 NN denotes weight
T15223 3340-3342 IN denotes of
T15224 3343-3347 NN denotes HOIP
T15225 3348-3350 VB denotes is
T15226 3351-3354 CD denotes 120
T15227 3355-3357 NN denotes kD
T15490 4763-4776 JJ denotes CD40-mediated
T15491 4777-4781 NN denotes CD80
T15492 4782-4794 NN denotes upregulation
T15493 4795-4797 VB denotes is
T15494 4798-4807 JJ denotes defective
T15495 4808-4810 IN denotes in
T15496 4811-4825 JJ denotes HOIP-deficient
T15497 4826-4831 NN denotes cells
T15498 4833-4838 NN denotes Cells
T15499 4839-4843 VB denotes were
T15500 4844-4853 VB denotes incubated
T15501 4854-4857 IN denotes for
T15502 4858-4860 CD denotes 72
T15503 4861-4864 NN denotes hrs
T15504 4865-4869 IN denotes with
T15505 4870-4872 DT denotes an
T15506 4873-4880 NN denotes isotype
T15507 4881-4888 NN denotes control
T15508 4889-4897 NN denotes antibody
T15509 4898-4899 -LRB- denotes (
T15510 4899-4903 JJ denotes left
T15511 4903-4904 -RRB- denotes )
T15512 4905-4907 CC denotes or
T15513 4908-4917 JJ denotes anti-CD40
T15514 4918-4926 NN denotes antibody
T15515 4927-4928 -LRB- denotes (
T15516 4928-4933 NN denotes right
T15517 4933-4934 -RRB- denotes )
T15518 4935-4938 CC denotes and
T15519 4939-4943 RB denotes then
T15520 4944-4951 VB denotes stained
T15521 4952-4955 IN denotes for
T15522 4956-4966 NN denotes expression
T15523 4967-4969 IN denotes of
T15524 4970-4974 NN denotes CD80
T15525 4975-4976 -LRB- denotes (
T15526 4976-4982 VB denotes filled
T15527 4983-4991 NN denotes profiles
T15528 4992-5000 VB denotes indicate
T15529 5001-5009 NN denotes staining
T15530 5010-5014 IN denotes with
T15531 5015-5024 JJ denotes anti-CD80
T15532 5025-5033 NN denotes antibody
T15533 5033-5034 -COLON- denotes ;
T15534 5035-5039 JJ denotes open
T15535 5040-5048 NN denotes profiles
T15536 5049-5052 VB denotes are
T15537 5053-5061 VB denotes staining
T15538 5062-5066 IN denotes with
T15539 5067-5069 DT denotes an
T15540 5070-5077 NN denotes isotype
T15541 5078-5085 NN denotes control
T15542 5086-5094 NN denotes antibody
T15543 5094-5095 -RRB- denotes )
T15544 5097-5109 NN denotes Transduction
T15545 5110-5112 IN denotes of
T15546 5113-5117 CC denotes both
T15547 5118-5132 JJ denotes HOIP-deficient
T15548 5133-5134 -LRB- denotes (
T15549 5134-5141 JJ denotes HOIP-/-
T15550 5141-5142 -RRB- denotes )
T15551 5143-5149 NN denotes clones
T15552 5150-5154 IN denotes with
T15553 5155-5156 DT denotes a
T15554 5157-5167 NN denotes retrovirus
T15555 5168-5176 VB denotes encoding
T15556 5177-5186 NN denotes FLAG-HOIP
T15557 5187-5195 VB denotes reversed
T15558 5196-5205 JJ denotes defective
T15559 5206-5210 NN denotes CD80
T15560 5211-5223 NN denotes upregulation
T15561 5223-5224 -COMMA- denotes ,
T15562 5225-5230 IN denotes while
T15563 5231-5243 NN denotes transduction
T15564 5244-5248 IN denotes with
T15565 5249-5251 DT denotes an
T15566 5252-5257 JJ denotes empty
T15567 5258-5264 NN denotes vector
T15568 5265-5266 -LRB- denotes (
T15569 5266-5270 NN denotes pMIP
T15570 5270-5271 -RRB- denotes )
T15571 5272-5275 VB denotes did
T15572 5276-5279 RB denotes not
T15573 5281-5288 JJ denotes Similar
T15574 5289-5296 NN denotes results
T15575 5297-5301 VB denotes were
T15576 5302-5310 VB denotes obtained
T15577 5311-5313 IN denotes in
T15578 5314-5315 DT denotes a
T15579 5316-5322 JJ denotes second
T15580 5323-5333 NN denotes experiment
T15889 6765-6768 NN denotes GLε
T15890 6769-6782 NN denotes transcription
T15891 6783-6785 VB denotes is
T15892 6786-6795 JJ denotes defective
T15893 6796-6798 IN denotes in
T15894 6799-6814 JJ denotes CD40-stimulated
T15895 6815-6829 JJ denotes HOIP-deficient
T15896 6830-6835 NN denotes cells
T15897 6837-6843 NN denotes A20.2J
T15898 6844-6847 CC denotes and
T15899 6848-6862 JJ denotes HOIP-deficient
T15900 6863-6868 NN denotes cells
T15901 6869-6879 VB denotes transduced
T15902 6880-6884 IN denotes with
T15903 6885-6887 DT denotes an
T15904 6888-6893 JJ denotes empty
T15905 6894-6904 JJ denotes retroviral
T15906 6905-6911 NN denotes vector
T15907 6912-6913 -LRB- denotes (
T15908 6913-6919 NN denotes A20.2J
T15909 6920-6921 SYM denotes +
T15910 6922-6926 NN denotes pMIP
T15911 6927-6930 CC denotes and
T15912 6931-6938 JJ denotes HOIP-/-
T15913 6939-6940 SYM denotes +
T15914 6941-6945 NN denotes pMIP
T15915 6945-6946 -COMMA- denotes ,
T15916 6947-6959 RB denotes respectively
T15917 6959-6960 -RRB- denotes )
T15918 6960-6961 -COMMA- denotes ,
T15919 6962-6964 CC denotes or
T15920 6965-6979 JJ denotes HOIP-deficient
T15921 6980-6985 NN denotes cells
T15922 6986-6996 VB denotes transduced
T15923 6997-7001 IN denotes with
T15924 7002-7003 DT denotes a
T15925 7004-7017 JJ denotes HOIP-encoding
T15926 7018-7028 NN denotes retrovirus
T15927 7029-7030 -LRB- denotes (
T15928 7030-7037 JJ denotes HOIP-/-
T15929 7038-7039 SYM denotes +
T15930 7040-7044 NN denotes HOIP
T15931 7044-7045 -RRB- denotes )
T15932 7046-7050 VB denotes were
T15933 7051-7059 VB denotes cultured
T15934 7060-7069 RB denotes overnight
T15935 7070-7074 IN denotes with
T15936 7075-7084 JJ denotes agonistic
T15937 7085-7094 NN denotes anti-CD40
T15938 7095-7097 CC denotes or
T15939 7098-7100 DT denotes an
T15940 7101-7108 NN denotes isotype
T15941 7109-7116 NN denotes control
T15942 7117-7125 NN denotes antibody
T15943 7126-7127 -LRB- denotes (
T15944 7127-7130 NN denotes iso
T15945 7130-7131 -RRB- denotes )
T15946 7131-7132 -COMMA- denotes ,
T15947 7133-7137 IN denotes with
T15948 7138-7140 CC denotes or
T15949 7141-7148 IN denotes without
T15950 7149-7153 NN denotes IL-4
T15951 7155-7158 NN denotes RNA
T15952 7159-7162 VB denotes was
T15953 7163-7171 VB denotes isolated
T15954 7172-7176 IN denotes from
T15955 7177-7180 DT denotes the
T15956 7181-7186 NN denotes cells
T15957 7186-7187 -COMMA- denotes ,
T15958 7188-7207 JJ denotes reverse-transcribed
T15959 7207-7208 -COMMA- denotes ,
T15960 7209-7212 CC denotes and
T15961 7213-7217 RB denotes then
T15962 7218-7227 VB denotes subjected
T15963 7228-7230 TO denotes to
T15964 7231-7243 JJ denotes quantitative
T15965 7244-7247 NN denotes PCR
T15966 7248-7250 TO denotes to
T15967 7251-7260 VB denotes determine
T15968 7261-7267 NN denotes levels
T15969 7268-7270 IN denotes of
T15970 7271-7274 NN denotes GLε
T15971 7275-7286 NN denotes transcripts
T15972 7288-7295 NN denotes Results
T15973 7296-7300 VB denotes were
T15974 7301-7311 VB denotes normalized
T15975 7312-7314 TO denotes to
T15976 7315-7318 DT denotes the
T15977 7319-7325 NN denotes levels
T15978 7326-7328 IN denotes of
T15979 7329-7334 NN denotes Hprt1
T15980 7335-7346 NN denotes transcripts
T15981 7347-7349 IN denotes in
T15982 7350-7354 DT denotes each
T15983 7355-7361 NN denotes sample
T15984 7363-7370 NN denotes Symbols
T15985 7371-7379 VB denotes indicate
T15986 7380-7383 DT denotes the
T15987 7384-7390 NN denotes values
T15988 7391-7395 IN denotes from
T15989 7396-7405 NN denotes duplicate
T15990 7406-7414 NN denotes cultures
T15991 7415-7416 -LRB- denotes (
T15992 7416-7417 DT denotes a
T15993 7418-7422 NN denotes line
T15994 7423-7432 VB denotes indicates
T15995 7433-7436 DT denotes the
T15996 7437-7441 NN denotes mean
T15997 7442-7444 IN denotes of
T15998 7445-7448 DT denotes the
T15999 7449-7452 CD denotes two
T16000 7453-7459 NN denotes values
T16001 7459-7460 -RRB- denotes )
T16002 7462-7469 JJ denotes Similar
T16003 7470-7477 NN denotes results
T16004 7478-7482 VB denotes were
T16005 7483-7491 VB denotes obtained
T16006 7492-7494 IN denotes in
T16007 7495-7496 DT denotes a
T16008 7497-7503 JJ denotes second
T16009 7504-7514 NN denotes experiment
T16010 7515-7518 CC denotes and
T16011 7519-7521 IN denotes in
T16012 7522-7524 DT denotes an
T16013 7525-7535 JJ denotes additional
T16014 7536-7546 NN denotes experiment
T16015 7547-7551 IN denotes with
T16016 7552-7553 DT denotes a
T16017 7554-7560 JJ denotes second
T16018 7561-7575 JJ denotes HOIP-deficient
T16019 7576-7581 NN denotes clone
T16374 9287-9299 JJ denotes CD40-induced
T16375 9300-9310 NN denotes activation
T16376 9311-9313 IN denotes of
T16377 9314-9319 NN denotes NF-κB
T16378 9320-9323 CC denotes and
T16379 9324-9327 NN denotes JNK
T16380 9328-9330 VB denotes is
T16381 9331-9340 JJ denotes defective
T16382 9341-9343 IN denotes in
T16383 9344-9358 JJ denotes HOIP-deficient
T16384 9359-9364 NN denotes cells
T16385 9366-9379 JJ denotes CD40-mediated
T16386 9380-9389 NN denotes signaling
T16387 9390-9393 VB denotes was
T16388 9394-9403 JJ denotes defective
T16389 9404-9406 IN denotes in
T16390 9407-9421 JJ denotes HOIP-deficient
T16391 9422-9427 NN denotes cells
T16392 9428-9429 -LRB- denotes (
T16393 9429-9436 JJ denotes HOIP-/-
T16394 9437-9438 SYM denotes +
T16395 9439-9443 NN denotes pMIP
T16396 9443-9444 -RRB- denotes )
T16397 9444-9445 -COMMA- denotes ,
T16398 9446-9449 CC denotes but
T16399 9450-9456 JJ denotes intact
T16400 9457-9459 IN denotes in
T16401 9460-9468 JJ denotes parental
T16402 9469-9474 NN denotes cells
T16403 9475-9485 VB denotes transduced
T16404 9486-9490 IN denotes with
T16405 9491-9493 DT denotes an
T16406 9494-9499 JJ denotes empty
T16407 9500-9510 JJ denotes retroviral
T16408 9511-9517 NN denotes vector
T16409 9518-9519 -LRB- denotes (
T16410 9519-9525 NN denotes A20.2J
T16411 9526-9527 SYM denotes +
T16412 9528-9532 NN denotes pMIP
T16413 9532-9533 -RRB- denotes )
T16414 9534-9537 CC denotes and
T16415 9538-9540 IN denotes in
T16416 9541-9555 JJ denotes HOIP-deficient
T16417 9556-9561 NN denotes cells
T16418 9562-9572 VB denotes transduced
T16419 9573-9577 IN denotes with
T16420 9578-9579 DT denotes a
T16421 9580-9590 JJ denotes retroviral
T16422 9591-9597 NN denotes vector
T16423 9598-9606 VB denotes encoding
T16424 9607-9611 NN denotes HOIP
T16425 9612-9613 -LRB- denotes (
T16426 9613-9620 JJ denotes HOIP-/-
T16427 9621-9622 SYM denotes +
T16428 9623-9627 NN denotes HOIP
T16429 9627-9628 -RRB- denotes )
T16430 9630-9635 NN denotes Cells
T16431 9636-9640 VB denotes were
T16432 9641-9650 VB denotes activated
T16433 9651-9655 IN denotes with
T16434 9656-9672 JJ denotes CD154-expressing
T16435 9673-9679 NN denotes insect
T16436 9680-9685 NN denotes cells
T16437 9686-9689 IN denotes for
T16438 9690-9693 DT denotes the
T16439 9694-9699 NN denotes times
T16440 9700-9709 VB denotes indicated
T16441 9711-9713 IN denotes As
T16442 9714-9715 DT denotes a
T16443 9716-9724 JJ denotes negative
T16444 9725-9732 NN denotes control
T16445 9732-9733 -COMMA- denotes ,
T16446 9734-9739 NN denotes cells
T16447 9740-9744 VB denotes were
T16448 9745-9755 VB denotes stimulated
T16449 9756-9760 IN denotes with
T16450 9761-9767 NN denotes insect
T16451 9768-9773 NN denotes cells
T16452 9774-9781 VB denotes lacking
T16453 9782-9787 NN denotes CD154
T16454 9788-9789 -LRB- denotes (
T16455 9789-9790 CD denotes 5
T16456 9791-9797 JJ denotes minute
T16457 9798-9802 NN denotes time
T16458 9803-9808 NN denotes point
T16459 9809-9813 RB denotes only
T16460 9813-9814 -RRB- denotes )
T16461 9816-9823 JJ denotes Western
T16462 9824-9829 NN denotes blots
T16463 9830-9832 IN denotes of
T16464 9833-9843 JJ denotes whole-cell
T16465 9844-9851 NN denotes lysates
T16466 9852-9856 VB denotes were
T16467 9857-9863 VB denotes probed
T16468 9864-9868 IN denotes with
T16469 9869-9872 DT denotes the
T16470 9873-9882 JJ denotes indicated
T16471 9883-9893 NN denotes antibodies
T16472 9895-9907 NN denotes Phospho-IκBα
T16473 9908-9909 -LRB- denotes (
T16474 9909-9914 NN denotes pIκBα
T16475 9914-9915 -RRB- denotes )
T16476 9916-9919 CC denotes and
T16477 9920-9931 NN denotes phospho-JNK
T16478 9932-9933 -LRB- denotes (
T16479 9933-9937 NN denotes pJNK
T16480 9937-9938 -RRB- denotes )
T16481 9939-9944 NN denotes blots
T16482 9945-9949 VB denotes were
T16483 9950-9958 VB denotes stripped
T16484 9959-9962 CC denotes and
T16485 9963-9971 VB denotes reprobed
T16486 9972-9975 IN denotes for
T16487 9976-9981 JJ denotes total
T16488 9982-9986 NN denotes IκBα
T16489 9987-9990 CC denotes and
T16490 9991-9996 JJ denotes total
T16491 9997-10000 NN denotes JNK
T16492 10000-10001 -COMMA- denotes ,
T16493 10002-10014 RB denotes respectively
T16494 10015-10016 -LRB- denotes (
T16495 10016-10024 JJ denotes anti-JNK
T16496 10025-10035 NN denotes antibodies
T16497 10036-10045 VB denotes recognize
T16498 10046-10049 NN denotes p46
T16499 10050-10053 CC denotes and
T16500 10054-10057 NN denotes p54
T16501 10058-10066 NN denotes isoforms
T16502 10066-10067 -RRB- denotes )
T16503 10069-10073 NN denotes IκBα
T16504 10074-10079 NN denotes blots
T16505 10080-10084 VB denotes were
T16506 10085-10089 RB denotes also
T16507 10090-10098 VB denotes reprobed
T16508 10099-10102 IN denotes for
T16509 10103-10108 NN denotes actin
T16510 10109-10111 TO denotes to
T16511 10112-10123 VB denotes demonstrate
T16512 10124-10129 JJ denotes equal
T16513 10130-10134 NN denotes lane
T16514 10135-10142 NN denotes loading
T16515 10144-10153 JJ denotes Molecular
T16516 10154-10161 NN denotes weights
T16517 10162-10165 VB denotes are
T16518 10166-10175 VB denotes indicated
T16519 10176-10178 IN denotes at
T16520 10179-10184 NN denotes right
T16521 10186-10193 JJ denotes Similar
T16522 10194-10201 NN denotes results
T16523 10202-10206 VB denotes were
T16524 10207-10215 VB denotes obtained
T16525 10216-10218 IN denotes in
T16526 10219-10220 DT denotes a
T16527 10221-10227 JJ denotes second
T16528 10228-10238 NN denotes experiment
T16529 10239-10242 CC denotes and
T16530 10243-10245 IN denotes in
T16531 10246-10249 CD denotes two
T16532 10250-10260 JJ denotes additional
T16533 10261-10272 NN denotes experiments
T16534 10273-10277 IN denotes with
T16535 10278-10279 DT denotes a
T16536 10280-10286 JJ denotes second
T16537 10287-10301 JJ denotes HOIP-deficient
T16538 10302-10307 NN denotes clone
T17281 12073-12076 NN denotes IKK
T17282 12077-12088 NN denotes recruitment
T17283 12089-12091 TO denotes to
T17284 12092-12095 DT denotes the
T17285 12096-12100 NN denotes CD40
T17286 12101-12110 NN denotes signaling
T17287 12111-12118 NN denotes complex
T17288 12119-12121 VB denotes is
T17289 12122-12131 JJ denotes defective
T17290 12132-12134 IN denotes in
T17291 12135-12149 JJ denotes HOIP-deficient
T17292 12150-12155 NN denotes cells
T17293 12157-12158 -LRB- denotes (
T17294 12158-12159 NN denotes A
T17295 12159-12160 -RRB- denotes )
T17296 12161-12165 NN denotes SMAC
T17297 12166-12173 NN denotes peptide
T17298 12174-12183 NN denotes treatment
T17299 12184-12191 VB denotes reduces
T17300 12192-12203 NN denotes recruitment
T17301 12204-12206 IN denotes of
T17302 12207-12212 NN denotes cIAP1
T17303 12213-12215 TO denotes to
T17304 12216-12220 NN denotes CD40
T17305 12221-12224 CC denotes and
T17306 12225-12228 MD denotes may
T17307 12229-12235 VB denotes modify
T17308 12236-12240 NN denotes HOIP
T17309 12241-12252 NN denotes recruitment
T17310 12254-12260 NN denotes A20.2J
T17311 12261-12266 NN denotes cells
T17312 12267-12271 VB denotes were
T17313 12272-12281 VB denotes incubated
T17314 12282-12285 IN denotes for
T17315 12286-12289 CD denotes six
T17316 12290-12295 NN denotes hours
T17317 12296-12300 IN denotes with
T17318 12301-12319 JJ denotes membrane-permeable
T17319 12320-12327 NN denotes SMAC-N7
T17320 12328-12335 NN denotes peptide
T17321 12336-12338 CC denotes or
T17322 12339-12342 CD denotes 1.5
T17323 12342-12343 NN denotes %
T17324 12344-12348 NN denotes DMSO
T17325 12349-12350 -LRB- denotes (
T17326 12350-12357 NN denotes solvent
T17327 12358-12362 VB denotes used
T17328 12363-12366 IN denotes for
T17329 12367-12370 DT denotes the
T17330 12371-12378 NN denotes peptide
T17331 12378-12379 -RRB- denotes )
T17332 12381-12390 VB denotes Following
T17333 12391-12394 DT denotes the
T17334 12395-12405 NN denotes incubation
T17335 12405-12406 -COMMA- denotes ,
T17336 12407-12411 NN denotes cell
T17337 12412-12419 NN denotes lysates
T17338 12420-12424 VB denotes were
T17339 12425-12433 VB denotes prepared
T17340 12433-12434 -COMMA- denotes ,
T17341 12435-12447 VB denotes fractionated
T17342 12448-12450 IN denotes by
T17343 12451-12459 NN denotes SDS-PAGE
T17344 12459-12460 -COMMA- denotes ,
T17345 12461-12464 CC denotes and
T17346 12465-12474 VB denotes evaluated
T17347 12475-12477 IN denotes by
T17348 12478-12485 JJ denotes Western
T17349 12486-12490 NN denotes blot
T17350 12491-12492 -LRB- denotes (
T17351 12492-12497 NN denotes lanes
T17352 12498-12499 CD denotes 1
T17353 12500-12503 CC denotes and
T17354 12504-12505 CD denotes 2
T17355 12505-12506 -RRB- denotes )
T17356 12508-12513 NN denotes Cells
T17357 12514-12523 VB denotes incubated
T17358 12524-12528 IN denotes with
T17359 12529-12533 NN denotes DMSO
T17360 12534-12536 CC denotes or
T17361 12537-12544 NN denotes SMAC-N7
T17362 12545-12549 VB denotes were
T17363 12550-12554 RB denotes also
T17364 12555-12565 VB denotes stimulated
T17365 12566-12570 IN denotes with
T17366 12571-12579 JJ denotes magnetic
T17367 12580-12585 NN denotes beads
T17368 12586-12592 VB denotes coated
T17369 12593-12597 IN denotes with
T17370 12598-12607 NN denotes anti-CD40
T17371 12608-12610 CC denotes or
T17372 12611-12613 DT denotes an
T17373 12614-12621 NN denotes isotype
T17374 12622-12629 NN denotes control
T17375 12630-12638 NN denotes antibody
T17376 12640-12658 VB denotes Immunoprecipitated
T17377 12659-12660 -LRB- denotes (
T17378 12660-12662 NN denotes IP
T17379 12662-12663 -RRB- denotes )
T17380 12664-12672 NN denotes material
T17381 12673-12678 VB denotes bound
T17382 12679-12681 TO denotes to
T17383 12682-12685 DT denotes the
T17384 12686-12691 NN denotes beads
T17385 12692-12695 VB denotes was
T17386 12696-12702 VB denotes loaded
T17387 12703-12705 IN denotes in
T17388 12706-12711 NN denotes lanes
T17389 12712-12715 CD denotes 3-5
T17390 12717-12724 NN denotes Samples
T17391 12725-12727 IN denotes of
T17392 12728-12731 DT denotes the
T17393 12732-12736 NN denotes cell
T17394 12737-12744 NN denotes lysates
T17395 12745-12750 IN denotes after
T17396 12751-12770 NN denotes immunoprecipitation
T17397 12771-12777 VB denotes appear
T17398 12778-12780 IN denotes in
T17399 12781-12786 NN denotes lanes
T17400 12787-12790 CD denotes 6–8
T17401 12792-12799 JJ denotes Western
T17402 12800-12805 NN denotes blots
T17403 12806-12810 VB denotes were
T17404 12811-12817 VB denotes probed
T17405 12818-12822 IN denotes with
T17406 12823-12833 NN denotes antibodies
T17407 12834-12842 JJ denotes specific
T17408 12843-12846 IN denotes for
T17409 12847-12852 NN denotes cIAP1
T17410 12852-12853 -COMMA- denotes ,
T17411 12854-12859 NN denotes TRAF2
T17412 12859-12860 -COMMA- denotes ,
T17413 12861-12866 NN denotes TRAF3
T17414 12866-12867 -COMMA- denotes ,
T17415 12868-12871 CC denotes and
T17416 12872-12876 NN denotes HOIP
T17417 12877-12878 -LRB- denotes (
T17418 12878-12889 JJ denotes approximate
T17419 12890-12899 JJ denotes molecular
T17420 12900-12907 NN denotes weights
T17421 12908-12917 VB denotes indicated
T17422 12918-12920 IN denotes on
T17423 12921-12925 JJ denotes left
T17424 12925-12926 -RRB- denotes )
T17425 12928-12935 JJ denotes Similar
T17426 12936-12943 NN denotes results
T17427 12944-12948 VB denotes were
T17428 12949-12957 VB denotes obtained
T17429 12958-12960 IN denotes in
T17430 12961-12964 CD denotes two
T17431 12965-12975 JJ denotes additional
T17432 12976-12988 NN denotes experiments.
T17433 12989-12990 -LRB- denotes (
T17434 12990-12991 NN denotes B
T17435 12991-12992 -RRB- denotes )
T17436 12993-12997 NN denotes CD40
T17437 12998-13001 VB denotes was
T17438 13002-13010 VB denotes isolated
T17439 13011-13013 IN denotes by
T17440 13014-13033 NN denotes immunoprecipitation
T17441 13034-13035 -LRB- denotes (
T17442 13035-13037 IN denotes as
T17443 13038-13040 IN denotes in
T17444 13041-13042 -LRB- denotes (
T17445 13042-13043 NN denotes A
T17446 13043-13044 -RRB- denotes )
T17447 13044-13045 -RRB- denotes )
T17448 13046-13050 IN denotes from
T17449 13051-13057 NN denotes A20.2J
T17450 13058-13063 NN denotes cells
T17451 13064-13067 CC denotes and
T17452 13068-13082 JJ denotes HOIP-deficient
T17453 13083-13088 NN denotes cells
T17454 13089-13099 VB denotes transduced
T17455 13100-13104 IN denotes with
T17456 13105-13107 DT denotes an
T17457 13108-13113 JJ denotes empty
T17458 13114-13124 JJ denotes retroviral
T17459 13125-13131 NN denotes vector
T17460 13132-13133 -LRB- denotes (
T17461 13133-13137 NN denotes pMIP
T17462 13137-13138 -RRB- denotes )
T17463 13139-13141 CC denotes or
T17464 13142-13143 DT denotes a
T17465 13144-13154 JJ denotes retroviral
T17466 13155-13161 NN denotes vector
T17467 13162-13170 VB denotes encoding
T17468 13171-13175 NN denotes HOIP
T17469 13177-13185 NN denotes Material
T17470 13186-13204 VB denotes immunoprecipitated
T17471 13205-13209 IN denotes with
T17472 13210-13212 DT denotes an
T17473 13213-13220 NN denotes isotype
T17474 13221-13228 NN denotes control
T17475 13229-13237 NN denotes antibody
T17476 13238-13239 -LRB- denotes (
T17477 13239-13246 NN denotes isotype
T17478 13246-13247 -RRB- denotes )
T17479 13248-13250 CC denotes or
T17480 13251-13260 JJ denotes anti-CD40
T17481 13261-13269 NN denotes antibody
T17482 13270-13273 VB denotes was
T17483 13274-13282 VB denotes examined
T17484 13283-13285 IN denotes by
T17485 13286-13293 JJ denotes Western
T17486 13294-13302 NN denotes blotting
T17487 13303-13306 IN denotes for
T17488 13307-13311 NN denotes CD40
T17489 13311-13312 -COMMA- denotes ,
T17490 13313-13318 NN denotes TRAF2
T17491 13318-13319 -COMMA- denotes ,
T17492 13320-13325 NN denotes TRAF3
T17493 13325-13326 -COMMA- denotes ,
T17494 13327-13332 NN denotes cIAP1
T17495 13332-13333 -COMMA- denotes ,
T17496 13334-13338 NN denotes HOIP
T17497 13338-13339 -COMMA- denotes ,
T17498 13340-13346 NN denotes IKKα/β
T17499 13346-13347 -COMMA- denotes ,
T17500 13348-13351 CC denotes and
T17501 13352-13356 NN denotes IKKγ
T17502 13357-13358 -LRB- denotes (
T17503 13358-13363 JJ denotes right
T17504 13364-13370 NN denotes panels
T17505 13370-13371 -RRB- denotes )
T17506 13373-13377 NN denotes HOIP
T17507 13378-13388 NN denotes expression
T17508 13389-13392 VB denotes was
T17509 13393-13401 VB denotes required
T17510 13402-13405 IN denotes for
T17511 13406-13421 NN denotes coprecipitation
T17512 13422-13424 IN denotes of
T17513 13425-13428 NN denotes IKK
T17514 13429-13437 NN denotes proteins
T17515 13438-13442 IN denotes with
T17516 13443-13447 NN denotes CD40
T17517 13449-13453 NN denotes Cell
T17518 13454-13461 NN denotes lysates
T17519 13462-13466 IN denotes from
T17520 13467-13479 JJ denotes unstimulated
T17521 13480-13485 NN denotes cells
T17522 13486-13489 VB denotes are
T17523 13490-13495 VB denotes shown
T17524 13496-13498 IN denotes in
T17525 13499-13502 DT denotes the
T17526 13503-13507 JJ denotes left
T17527 13508-13514 NN denotes panels
T17528 13516-13523 JJ denotes Similar
T17529 13524-13531 NN denotes results
T17530 13532-13536 VB denotes were
T17531 13537-13545 VB denotes obtained
T17532 13546-13548 IN denotes in
T17533 13549-13550 DT denotes a
T17534 13551-13557 JJ denotes second
T17535 13558-13568 NN denotes experiment
T17536 13569-13572 CC denotes and
T17537 13573-13575 IN denotes in
T17538 13576-13579 CD denotes two
T17539 13580-13591 NN denotes experiments
T17540 13592-13596 IN denotes with
T17541 13597-13598 DT denotes a
T17542 13599-13605 JJ denotes second
T17543 13606-13620 JJ denotes HOIP-deficient
T17544 13621-13627 NN denotes clone.
T17545 13628-13629 -LRB- denotes (
T17546 13629-13630 NN denotes C
T17547 13630-13631 -RRB- denotes )
T17548 13632-13634 TO denotes To
T17549 13635-13642 RB denotes further
T17550 13643-13651 VB denotes evaluate
T17551 13652-13666 JJ denotes HOIP-dependent
T17552 13667-13678 NN denotes recruitment
T17553 13679-13681 IN denotes of
T17554 13682-13686 NN denotes IKKγ
T17555 13687-13689 TO denotes to
T17556 13690-13694 NN denotes CD40
T17557 13694-13695 -COMMA- denotes ,
T17558 13696-13702 NN denotes A20.2J
T17559 13703-13708 NN denotes cells
T17560 13709-13711 CC denotes or
T17561 13712-13726 JJ denotes HOIP-deficient
T17562 13727-13728 -LRB- denotes (
T17563 13728-13735 JJ denotes HOIP-/-
T17564 13735-13736 -RRB- denotes )
T17565 13737-13743 NN denotes A20.2J
T17566 13744-13749 NN denotes cells
T17567 13750-13754 VB denotes were
T17568 13755-13765 VB denotes transduced
T17569 13766-13770 IN denotes with
T17570 13771-13773 DT denotes an
T17571 13774-13779 JJ denotes empty
T17572 13780-13790 JJ denotes retroviral
T17573 13791-13797 NN denotes vector
T17574 13798-13800 CC denotes or
T17575 13801-13802 DT denotes a
T17576 13803-13813 JJ denotes retroviral
T17577 13814-13823 NN denotes construct
T17578 13824-13832 VB denotes encoding
T17579 13833-13835 NN denotes BP
T17580 13836-13850 JJ denotes epitope-tagged
T17581 13851-13855 NN denotes IKKγ
T17582 13856-13857 -LRB- denotes (
T17583 13857-13862 VB denotes noted
T17584 13863-13865 IN denotes in
T17585 13866-13869 DT denotes the
T17586 13870-13876 NN denotes figure
T17587 13877-13879 IN denotes as
T17588 13880-13884 NN denotes pMIP
T17589 13885-13888 CC denotes and
T17590 13889-13893 NN denotes IKKγ
T17591 13893-13894 -COMMA- denotes ,
T17592 13895-13907 RB denotes respectively
T17593 13907-13908 -RRB- denotes )
T17594 13910-13917 NN denotes Lysates
T17595 13918-13919 -LRB- denotes (
T17596 13919-13924 NN denotes lanes
T17597 13925-13928 CD denotes 1–3
T17598 13928-13929 -RRB- denotes )
T17599 13930-13933 CC denotes and
T17600 13934-13953 NN denotes immunoprecipitation
T17601 13954-13955 -LRB- denotes (
T17602 13955-13957 NN denotes IP
T17603 13957-13958 -RRB- denotes )
T17604 13959-13966 NN denotes samples
T17605 13967-13968 -LRB- denotes (
T17606 13968-13973 NN denotes lanes
T17607 13974-13978 CD denotes 4–11
T17608 13978-13979 -RRB- denotes )
T17609 13980-13984 IN denotes from
T17610 13985-13988 DT denotes the
T17611 13989-13993 NN denotes cell
T17612 13994-13999 NN denotes lines
T17613 14000-14004 VB denotes were
T17614 14005-14017 VB denotes fractionated
T17615 14018-14020 IN denotes by
T17616 14021-14029 NN denotes SDS-PAGE
T17617 14030-14033 CC denotes and
T17618 14034-14043 VB denotes evaluated
T17619 14044-14046 IN denotes by
T17620 14047-14054 JJ denotes Western
T17621 14055-14063 NN denotes blotting
T17622 14064-14068 IN denotes with
T17623 14069-14079 NN denotes antibodies
T17624 14080-14082 TO denotes to
T17625 14083-14086 DT denotes the
T17626 14087-14089 NN denotes BP
T17627 14090-14093 NN denotes tag
T17628 14094-14095 -LRB- denotes (
T17629 14095-14099 NN denotes IKKγ
T17630 14099-14100 -COMMA- denotes ,
T17631 14101-14106 JJ denotes upper
T17632 14107-14112 NN denotes panel
T17633 14112-14113 -RRB- denotes )
T17634 14114-14117 CC denotes and
T17635 14118-14123 NN denotes TRAF2
T17636 14125-14128 DT denotes The
T17637 14129-14138 JJ denotes anti-CD40
T17638 14139-14141 NN denotes IP
T17639 14142-14148 NN denotes sample
T17640 14149-14151 IN denotes in
T17641 14152-14156 NN denotes lane
T17642 14157-14159 CD denotes 11
T17643 14160-14163 VB denotes was
T17644 14164-14171 VB denotes treated
T17645 14172-14176 IN denotes with
T17646 14177-14178 NN denotes λ
T17647 14179-14190 NN denotes phosphatase
T17648 14190-14191 -COLON- denotes ;
T17649 14192-14195 DT denotes the
T17650 14196-14202 NN denotes sample
T17651 14203-14205 IN denotes in
T17652 14206-14210 NN denotes lane
T17653 14211-14213 CD denotes 10
T17654 14214-14217 VB denotes was
T17655 14218-14230 JJ denotes mock-treated
T17656 14232-14239 NN denotes Protein
T17657 14240-14247 NN denotes samples
T17658 14248-14249 -LRB- denotes (
T17659 14249-14254 CC denotes minus
T17660 14255-14260 DT denotes those
T17661 14261-14268 VB denotes treated
T17662 14269-14273 IN denotes with
T17663 14274-14285 NN denotes phosphatase
T17664 14285-14286 -RRB- denotes )
T17665 14287-14291 VB denotes were
T17666 14292-14296 RB denotes also
T17667 14297-14309 VB denotes fractionated
T17668 14310-14312 IN denotes on
T17669 14313-14314 DT denotes a
T17670 14315-14323 JJ denotes separate
T17671 14324-14327 NN denotes gel
T17672 14328-14329 -LRB- denotes (
T17673 14329-14334 JJ denotes lower
T17674 14335-14345 NN denotes acrylamide
T17675 14346-14359 NN denotes concentration
T17676 14359-14360 -RRB- denotes )
T17677 14361-14364 IN denotes for
T17678 14365-14368 DT denotes the
T17679 14369-14379 NN denotes evaluation
T17680 14380-14382 IN denotes of
T17681 14383-14388 NN denotes TRAF3
T17682 14389-14392 CC denotes and
T17683 14393-14397 NN denotes HOIP
T17684 14398-14399 -LRB- denotes (
T17685 14399-14405 NN denotes bottom
T17686 14406-14409 CD denotes two
T17687 14410-14416 NN denotes panels
T17688 14416-14417 -RRB- denotes )
T17689 14419-14426 JJ denotes Similar
T17690 14427-14434 NN denotes results
T17691 14435-14439 VB denotes were
T17692 14440-14448 VB denotes obtained
T17693 14449-14451 IN denotes in
T17694 14452-14455 CD denotes two
T17695 14456-14466 JJ denotes additional
T17696 14467-14478 NN denotes experiments
R2586 T3341 T3342 arg1Of Generation,of
R2587 T3341 T3346 arg1Of Generation,via
R2588 T3345 T3342 arg2Of cells,of
R2589 T3345 T3343 arg1Of cells,HOIP-deficient
R2590 T3345 T3344 arg1Of cells,B
R2591 T3348 T3346 arg2Of disruption,via
R2592 T3348 T3347 arg2Of disruption,targeted
R2593 T3348 T3349 arg1Of disruption,of
R2594 T3350 T3349 arg2Of Rnf31,of
R2595 T3350 T3351 arg1Of Rnf31,","
R2596 T3353 T3351 arg2Of gene,","
R2597 T3353 T3352 arg1Of gene,the
R2598 T3353 T3354 arg1Of gene,encoding
R2599 T3355 T3354 arg2Of HOIP,encoding
R2600 T3359 T3356 arg2Of study,In
R2601 T3359 T3357 arg1Of study,our
R2602 T3359 T3358 arg1Of study,previous
R2603 T3361 T3362 arg1Of we,demonstrated
R2604 T3362 T3356 arg1Of demonstrated,In
R2605 T3362 T3360 arg1Of demonstrated,","
R2606 T3364 T3365 arg1Of HOIP,is
R2607 T3364 T3366 arg2Of HOIP,recruited
R2608 T3366 T3362 arg2Of recruited,demonstrated
R2609 T3366 T3363 arg1Of recruited,that
R2610 T3366 T3365 arg2Of recruited,is
R2611 T3366 T3367 arg1Of recruited,to
R2612 T3366 T3372 arg1Of recruited,in
R2613 T3366 T3378 arg1Of recruited,[
R2614 T3371 T3367 arg2Of complex,to
R2615 T3371 T3368 arg1Of complex,the
R2616 T3371 T3369 arg1Of complex,CD40
R2617 T3371 T3370 arg1Of complex,signaling
R2618 T3377 T3372 arg2Of lines,in
R2619 T3377 T3373 arg1Of lines,two
R2620 T3377 T3374 arg1Of lines,mouse
R2621 T3377 T3375 arg1Of lines,B
R2622 T3377 T3376 arg1Of lines,cell
R2623 T3379 T3378 arg2Of 6,[
R2624 T3380 T3378 arg3Of ],[
R2625 T3382 T3381 arg1Of results,Similar
R2626 T3382 T3383 arg1Of results,were
R2627 T3382 T3384 arg2Of results,obtained
R2628 T3384 T3383 arg2Of obtained,were
R2629 T3384 T3385 arg1Of obtained,with
R2630 T3384 T3388 arg1Of obtained,","
R2631 T3384 T3389 modOf obtained,indicating
R2632 T3387 T3385 arg2Of splenocytes,with
R2633 T3387 T3386 arg1Of splenocytes,mouse
R2634 T3392 T3391 arg1Of interaction,the
R2635 T3392 T3393 arg1Of interaction,of
R2636 T3392 T3395 arg1Of interaction,with
R2637 T3392 T3397 arg1Of interaction,is
R2638 T3392 T3399 arg2Of interaction,limited
R2639 T3394 T3393 arg2Of CD40,of
R2640 T3396 T3395 arg2Of HOIP,with
R2641 T3399 T3389 arg2Of limited,indicating
R2642 T3399 T3390 arg1Of limited,that
R2643 T3399 T3397 arg2Of limited,is
R2644 T3399 T3398 arg1Of limited,not
R2645 T3399 T3400 arg1Of limited,to
R2646 T3404 T3400 arg2Of lines,to
R2647 T3404 T3401 arg2Of lines,transformed
R2648 T3404 T3402 arg1Of lines,B
R2649 T3404 T3403 arg1Of lines,cell
R2650 T3404 T3405 arg1Of lines,(
R2651 T3407 T3405 arg2Of observations,(
R2652 T3407 T3406 arg1Of observations,unpublished
R2653 T3408 T3405 arg3Of ),(
R2654 T3409 T3411 arg1Of We,found
R2655 T3411 T3410 arg1Of found,also
R2656 T3414 T3413 arg1Of recruitment,the
R2657 T3414 T3415 arg1Of recruitment,of
R2658 T3414 T3417 arg1Of recruitment,to
R2659 T3414 T3419 arg1Of recruitment,was
R2660 T3414 T3420 arg1Of recruitment,TRAF2-dependent
R2661 T3416 T3415 arg2Of HOIP,of
R2662 T3418 T3417 arg2Of CD40,to
R2663 T3419 T3412 arg1Of was,that
R2664 T3419 T3421 arg1Of was,and
R2665 T3420 T3419 arg2Of TRAF2-dependent,was
R2666 T3421 T3411 arg2Of and,found
R2667 T3422 T3421 arg2Of that,and
R2668 T3423 T3424 arg1Of overexpression,of
R2669 T3423 T3430 arg1Of overexpression,inhibited
R2670 T3428 T3424 arg2Of mutant,of
R2671 T3428 T3425 arg1Of mutant,a
R2672 T3428 T3426 arg2Of mutant,truncated
R2673 T3428 T3427 arg1Of mutant,HOIP
R2674 T3430 T3422 arg2Of inhibited,that
R2675 T3430 T3429 arg1Of inhibited,partially
R2676 T3433 T3430 arg2Of activation,inhibited
R2677 T3433 T3431 arg1Of activation,CD40-mediated
R2678 T3433 T3432 arg1Of activation,NF-κB
R2679 T3435 T3434 arg1Of results,These
R2680 T3435 T3436 arg1Of results,support
R2681 T3438 T3436 arg2Of hypothesis,support
R2682 T3438 T3437 arg1Of hypothesis,the
R2683 T3440 T3441 arg1Of HOIP,plays
R2684 T3441 T3438 arg2Of plays,hypothesis
R2685 T3441 T3439 arg1Of plays,that
R2686 T3441 T3444 arg1Of plays,in
R2687 T3443 T3441 arg2Of role,plays
R2688 T3443 T3442 arg1Of role,a
R2689 T3447 T3444 arg2Of transduction,in
R2690 T3447 T3445 arg1Of transduction,CD40
R2691 T3447 T3446 arg1Of transduction,signal
R2692 T3450 T3453 arg1Of define,and
R2693 T3452 T3450 arg2Of role,define
R2694 T3452 T3451 arg1Of role,the
R2695 T3453 T3448 arg1Of and,To
R2696 T3453 T3449 arg1Of and,further
R2697 T3454 T3453 arg2Of evaluate,and
R2698 T3456 T3454 arg2Of importance,evaluate
R2699 T3456 T3455 arg1Of importance,the
R2700 T3456 T3457 arg1Of importance,of
R2701 T3456 T3459 arg1Of importance,in
R2702 T3458 T3457 arg2Of HOIP,of
R2703 T3461 T3459 arg2Of signaling,in
R2704 T3461 T3460 arg1Of signaling,CD40
R2705 T3463 T3450 arg1Of we,define
R2706 T3463 T3454 arg1Of we,evaluate
R2707 T3463 T3464 arg1Of we,used
R2708 T3464 T3448 modOf used,To
R2709 T3464 T3462 arg1Of used,","
R2710 T3467 T3464 arg2Of gene,used
R2711 T3467 T3465 arg1Of gene,somatic
R2712 T3467 T3466 arg1Of gene,cell
R2713 T3467 T3468 arg1Of gene,targeting
R2714 T3468 T3469 modOf targeting,to
R2715 T3470 T3469 arg1Of disrupt,to
R2716 T3472 T3470 arg2Of gene,disrupt
R2717 T3472 T3471 arg1Of gene,the
R2718 T3472 T3473 arg1Of gene,encoding
R2719 T3473 T3475 arg1Of encoding,in
R2720 T3474 T3473 arg2Of HOIP,encoding
R2721 T3480 T3475 arg2Of line,in
R2722 T3480 T3476 arg1Of line,the
R2723 T3480 T3477 arg1Of line,mouse
R2724 T3480 T3478 arg1Of line,B
R2725 T3480 T3479 arg1Of line,cell
R2726 T3481 T3468 arg2Of A20.2J,targeting
R2727 T3484 T3482 arg1Of line,This
R2728 T3484 T3483 arg1Of line,cell
R2729 T3484 T3485 arg1Of line,has
R2730 T3484 T3486 arg1Of line,been
R2731 T3484 T3488 arg1Of line,useful
R2732 T3486 T3485 arg2Of been,has
R2733 T3486 T3514 arg1Of been,","
R2734 T3486 T3515 arg1Of been,[
R2735 T3486 T3518 arg1Of been,","
R2736 T3486 T3519 arg1Of been,[
R2737 T3488 T3486 arg2Of useful,been
R2738 T3488 T3487 arg1Of useful,particularly
R2739 T3488 T3489 arg1Of useful,in
R2740 T3488 T3497 arg1Of useful,to
R2741 T3491 T3489 arg2Of characterization,in
R2742 T3491 T3490 arg1Of characterization,the
R2743 T3491 T3492 arg1Of characterization,of
R2744 T3495 T3492 arg2Of mechanisms,of
R2745 T3495 T3493 arg1Of mechanisms,CD40
R2746 T3495 T3494 arg1Of mechanisms,signaling
R2747 T3497 T3496 arg1Of to,due
R2748 T3500 T3497 arg2Of ease,to
R2749 T3500 T3498 arg1Of ease,the
R2750 T3500 T3499 arg1Of ease,relative
R2751 T3500 T3501 arg2Of ease,with
R2752 T3500 T3502 arg1Of ease,which
R2753 T3504 T3503 arg1Of genome,its
R2754 T3504 T3505 arg1Of genome,can
R2755 T3504 T3506 arg1Of genome,be
R2756 T3504 T3507 arg2Of genome,modified
R2757 T3507 T3501 arg1Of modified,with
R2758 T3507 T3505 arg2Of modified,can
R2759 T3507 T3506 arg2Of modified,be
R2760 T3510 T3507 arg1Of recombination,modified
R2761 T3510 T3508 arg2Of recombination,by
R2762 T3510 T3509 arg1Of recombination,homologous
R2763 T3510 T3511 arg1Of recombination,[
R2764 T3512 T3511 arg2Of 7,[
R2765 T3513 T3511 arg3Of ],[
R2766 T3516 T3515 arg2Of 8,[
R2767 T3517 T3515 arg3Of ],[
R2768 T3520 T3519 arg2Of 9,[
R2769 T3521 T3519 arg3Of ],[
R2770 T3522 T3523 arg1Of We,used
R2771 T3522 T3541 arg1Of We,disrupt
R2772 T3524 T3525 arg1Of a,targeting
R2773 T3525 T3523 arg2Of targeting,used
R2774 T3525 T3524 arg1Of targeting,a
R2775 T3526 T3525 arg2Of vector,targeting
R2776 T3526 T3527 arg1Of vector,capable
R2777 T3527 T3528 arg1Of capable,of
R2778 T3529 T3528 arg2Of undergoing,of
R2779 T3529 T3532 arg1Of undergoing,with
R2780 T3531 T3529 arg2Of recombination,undergoing
R2781 T3531 T3530 arg1Of recombination,homologous
R2782 T3533 T3532 arg2Of Rnf31,with
R2783 T3533 T3534 arg1Of Rnf31,(
R2784 T3536 T3534 arg2Of gene,(
R2785 T3536 T3535 arg1Of gene,the
R2786 T3536 T3537 arg1Of gene,encoding
R2787 T3538 T3537 arg2Of HOIP,encoding
R2788 T3539 T3534 arg3Of ),(
R2789 T3541 T3523 arg3Of disrupt,used
R2790 T3541 T3540 arg1Of disrupt,to
R2791 T3544 T3541 arg2Of sequence,disrupt
R2792 T3544 T3542 arg1Of sequence,the
R2793 T3544 T3543 arg1Of sequence,coding
R2794 T3544 T3545 arg1Of sequence,of
R2795 T3544 T3548 arg1Of sequence,in
R2796 T3547 T3545 arg2Of gene,of
R2797 T3547 T3546 arg1Of gene,the
R2798 T3549 T3548 arg2Of exon,in
R2799 T3549 T3550 arg1Of exon,5
R2800 T3549 T3551 arg1Of exon,(
R2801 T3553 T3551 arg2Of 1A,(
R2802 T3553 T3552 arg1Of 1A,Fig.
R2803 T3554 T3551 arg3Of ),(
R2804 T3556 T3555 arg2Of introduction,Following
R2805 T3556 T3557 arg1Of introduction,of
R2806 T3559 T3557 arg2Of vector,of
R2807 T3559 T3558 arg1Of vector,the
R2808 T3563 T3561 arg1Of clones,the
R2809 T3563 T3562 arg1Of clones,neomycin-resistant
R2810 T3563 T3564 arg1Of clones,that
R2811 T3563 T3565 arg1Of clones,arose
R2812 T3563 T3566 arg1Of clones,were
R2813 T3563 T3567 arg2Of clones,screened
R2814 T3567 T3555 arg1Of screened,Following
R2815 T3567 T3560 arg1Of screened,","
R2816 T3567 T3566 arg2Of screened,were
R2817 T3570 T3567 arg1Of amplification,screened
R2818 T3570 T3568 arg2Of amplification,by
R2819 T3570 T3569 arg1Of amplification,PCR
R2820 T3570 T3571 arg1Of amplification,of
R2821 T3570 T3574 modOf amplification,to
R2822 T3573 T3571 arg2Of DNA,of
R2823 T3573 T3572 arg1Of DNA,genomic
R2824 T3575 T3574 arg1Of identify,to
R2825 T3576 T3575 arg2Of cells,identify
R2826 T3576 T3577 arg1Of cells,containing
R2827 T3581 T3577 arg2Of allele,containing
R2828 T3581 T3578 arg1Of allele,a
R2829 T3581 T3579 arg2Of allele,disrupted
R2830 T3581 T3580 arg1Of allele,Rnf31
R2831 T3583 T3582 arg1Of remove,To
R2832 T3588 T3583 arg2Of cassette,remove
R2833 T3588 T3584 arg1Of cassette,the
R2834 T3588 T3585 arg1Of cassette,selectable
R2835 T3588 T3586 arg1Of cassette,marker
R2836 T3588 T3587 arg1Of cassette,gene
R2837 T3588 T3589 arg1Of cassette,from
R2838 T3593 T3589 arg2Of allele,from
R2839 T3593 T3590 arg1Of allele,the
R2840 T3593 T3591 arg2Of allele,disrupted
R2841 T3593 T3592 arg1Of allele,Rnf31
R2842 T3597 T3595 arg1Of lines,recombinant
R2843 T3597 T3596 arg1Of lines,cell
R2844 T3597 T3598 arg1Of lines,were
R2845 T3597 T3600 arg2Of lines,transfected
R2846 T3600 T3582 modOf transfected,To
R2847 T3600 T3594 arg1Of transfected,","
R2848 T3600 T3598 arg2Of transfected,were
R2849 T3600 T3599 arg1Of transfected,transiently
R2850 T3600 T3601 arg1Of transfected,with
R2851 T3603 T3601 arg2Of plasmid,with
R2852 T3603 T3602 arg1Of plasmid,a
R2853 T3603 T3604 arg1Of plasmid,that
R2854 T3603 T3605 arg1Of plasmid,encodes
R2855 T3607 T3605 arg2Of recombinase,encodes
R2856 T3607 T3606 arg1Of recombinase,Cre
R2857 T3609 T3608 arg1Of step,This
R2858 T3609 T3610 arg1Of step,allowed
R2859 T3610 T3622 arg1Of allowed,","
R2860 T3610 T3623 modOf allowed,generating
R2861 T3611 T3610 arg2Of us,allowed
R2862 T3611 T3613 arg1Of us,perform
R2863 T3613 T3610 arg3Of perform,allowed
R2864 T3613 T3612 arg1Of perform,to
R2865 T3616 T3613 arg2Of round,perform
R2866 T3616 T3614 arg1Of round,a
R2867 T3616 T3615 arg1Of round,second
R2868 T3616 T3617 arg1Of round,of
R2869 T3618 T3619 arg1Of targeting,and
R2870 T3619 T3617 arg2Of and,of
R2871 T3621 T3619 arg2Of selection,and
R2872 T3621 T3620 arg1Of selection,drug
R2873 T3624 T3623 arg2Of cells,generating
R2874 T3624 T3625 arg2Of cells,in
R2875 T3624 T3626 arg1Of cells,which
R2876 T3628 T3627 arg1Of copies,both
R2877 T3628 T3629 arg1Of copies,of
R2878 T3628 T3631 arg1Of copies,were
R2879 T3628 T3632 arg2Of copies,disrupted
R2880 T3630 T3629 arg2Of Rnf31,of
R2881 T3632 T3625 arg1Of disrupted,in
R2882 T3632 T3631 arg2Of disrupted,were
R2883 T3637 T3633 arg1Of lines,Two
R2884 T3637 T3634 arg1Of lines,independent
R2885 T3637 T3635 arg1Of lines,clonal
R2886 T3637 T3636 arg1Of lines,cell
R2887 T3637 T3638 arg1Of lines,were
R2888 T3637 T3639 arg2Of lines,chosen
R2889 T3639 T3638 arg2Of chosen,were
R2890 T3639 T3640 arg1Of chosen,for
R2891 T3642 T3640 arg2Of analysis,for
R2892 T3642 T3641 arg1Of analysis,further
R2893 T3645 T3643 arg1Of expression,HOIP
R2894 T3645 T3644 arg1Of expression,protein
R2895 T3645 T3646 arg1Of expression,was
R2896 T3645 T3647 arg1Of expression,undetectable
R2897 T3646 T3652 arg1Of was,","
R2898 T3646 T3653 arg1Of was,as
R2899 T3646 T3666 arg1Of was,","
R2900 T3646 T3667 modOf was,demonstrating
R2901 T3647 T3646 arg2Of undetectable,was
R2902 T3647 T3648 arg1Of undetectable,in
R2903 T3651 T3648 arg2Of lines,in
R2904 T3651 T3649 arg1Of lines,both
R2905 T3651 T3650 arg1Of lines,cell
R2906 T3654 T3653 arg2Of determined,as
R2907 T3658 T3654 arg1Of analysis,determined
R2908 T3658 T3655 arg2Of analysis,by
R2909 T3658 T3656 arg1Of analysis,Western
R2910 T3658 T3657 arg1Of analysis,blot
R2911 T3658 T3659 arg1Of analysis,of
R2912 T3661 T3659 arg2Of lysates,of
R2913 T3661 T3660 arg1Of lysates,cell
R2914 T3661 T3662 arg1Of lysates,(
R2915 T3664 T3662 arg2Of 1B,(
R2916 T3664 T3663 arg1Of 1B,Fig.
R2917 T3665 T3662 arg3Of ),(
R2918 T3669 T3670 arg1Of the,targeting
R2919 T3670 T3669 arg1Of targeting,the
R2920 T3670 T3672 arg1Of targeting,was
R2921 T3670 T3673 arg1Of targeting,successful
R2922 T3671 T3670 arg2Of process,targeting
R2923 T3672 T3667 arg2Of was,demonstrating
R2924 T3672 T3668 arg1Of was,that
R2925 T3673 T3672 arg2Of successful,was
R2926 T3676 T3674 arg2Of text,In
R2927 T3676 T3675 arg1Of text,the
R2928 T3676 T3677 arg1Of text,that
R2929 T3676 T3678 arg1Of text,follows
R2930 T3684 T3680 arg1Of lines,the
R2931 T3684 T3681 arg1Of lines,two
R2932 T3684 T3682 arg1Of lines,gene-targeted
R2933 T3684 T3683 arg1Of lines,cell
R2934 T3684 T3685 arg1Of lines,are
R2935 T3684 T3686 arg2Of lines,referred
R2936 T3686 T3674 arg1Of referred,In
R2937 T3686 T3679 arg1Of referred,","
R2938 T3686 T3685 arg2Of referred,are
R2939 T3686 T3687 arg1Of referred,to
R2940 T3686 T3688 arg1Of referred,as
R2941 T3690 T3688 arg2Of cells,as
R2942 T3690 T3689 arg1Of cells,HOIP-deficient
R2943 T3692 T3691 arg1Of enable,To
R2944 T3693 T3692 arg2Of us,enable
R2945 T3693 T3695 arg1Of us,confirm
R2946 T3695 T3692 arg3Of confirm,enable
R2947 T3695 T3694 arg1Of confirm,to
R2948 T3698 T3699 arg1Of signaling,or
R2949 T3700 T3699 arg2Of functional,or
R2950 T3701 T3697 arg1Of defects,any
R2951 T3701 T3698 arg1Of defects,signaling
R2952 T3701 T3700 arg1Of defects,functional
R2953 T3701 T3702 arg2Of defects,observed
R2954 T3701 T3706 arg1Of defects,were
R2955 T3701 T3707 arg1Of defects,due
R2956 T3702 T3703 arg1Of observed,in
R2957 T3705 T3703 arg2Of cells,in
R2958 T3705 T3704 arg1Of cells,HOIP-deficient
R2959 T3706 T3695 arg2Of were,confirm
R2960 T3706 T3696 arg1Of were,that
R2961 T3707 T3706 arg2Of due,were
R2962 T3707 T3708 arg1Of due,to
R2963 T3709 T3708 arg2Of disruption,to
R2964 T3709 T3710 arg1Of disruption,of
R2965 T3713 T3710 arg2Of gene,of
R2966 T3713 T3711 arg1Of gene,the
R2967 T3713 T3712 arg1Of gene,Rnf31
R2968 T3718 T3715 arg1Of lines,both
R2969 T3718 T3716 arg1Of lines,HOIP-deficient
R2970 T3718 T3717 arg1Of lines,cell
R2971 T3718 T3719 arg1Of lines,were
R2972 T3718 T3720 arg2Of lines,transduced
R2973 T3718 T3730 arg1Of lines,restoring
R2974 T3720 T3691 modOf transduced,To
R2975 T3720 T3714 arg1Of transduced,","
R2976 T3720 T3719 arg2Of transduced,were
R2977 T3720 T3721 arg1Of transduced,with
R2978 T3720 T3728 arg1Of transduced,","
R2979 T3720 T3729 arg1Of transduced,thus
R2980 T3720 T3730 modOf transduced,restoring
R2981 T3723 T3721 arg2Of retrovirus,with
R2982 T3723 T3722 arg1Of retrovirus,a
R2983 T3723 T3724 arg1Of retrovirus,encoding
R2984 T3727 T3724 arg2Of HOIP,encoding
R2985 T3727 T3725 arg1Of HOIP,FLAG-tagged
R2986 T3727 T3726 arg1Of HOIP,wild-type
R2987 T3733 T3730 arg2Of expression,restoring
R2988 T3733 T3731 arg1Of expression,HOIP
R2989 T3733 T3732 arg1Of expression,protein
R2990 T3733 T3734 arg1Of expression,(
R2991 T3736 T3734 arg2Of 1B,(
R2992 T3736 T3735 arg1Of 1B,Fig.
R2993 T3737 T3734 arg3Of ),(
R2994 T3739 T3738 arg1Of cells,These
R2995 T3739 T3740 arg1Of cells,are
R2996 T3739 T3741 arg2Of cells,referred
R2997 T3741 T3740 arg2Of referred,are
R2998 T3741 T3742 arg1Of referred,to
R2999 T3741 T3743 arg1Of referred,as
R3000 T3745 T3743 arg2Of cells,as
R3001 T3745 T3744 arg1Of cells,HOIP-reconstituted
R3002 T3748 T3746 arg2Of experiments,In
R3003 T3748 T3747 arg1Of experiments,the
R3004 T3748 T3749 arg1Of experiments,that
R3005 T3748 T3750 arg1Of experiments,follow
R3006 T3752 T3753 arg1Of responses,by
R3007 T3752 T3756 arg1Of responses,were
R3008 T3752 T3757 arg2Of responses,compared
R3009 T3755 T3753 arg2Of cells,by
R3010 T3755 T3754 arg1Of cells,these
R3011 T3757 T3746 arg1Of compared,In
R3012 T3757 T3751 arg1Of compared,","
R3013 T3757 T3756 arg2Of compared,were
R3014 T3757 T3758 arg1Of compared,to
R3015 T3759 T3758 arg2Of those,to
R3016 T3759 T3760 arg1Of those,of
R3017 T3762 T3761 arg1Of cells,A20.2J
R3018 T3762 T3763 arg1Of cells,and
R3019 T3763 T3760 arg2Of and,of
R3020 T3763 T3766 arg2Of and,transduced
R3021 T3765 T3763 arg2Of cells,and
R3022 T3765 T3764 arg1Of cells,HOIP-deficient
R3023 T3766 T3767 arg1Of transduced,with
R3024 T3770 T3767 arg2Of vector,with
R3025 T3770 T3768 arg1Of vector,a
R3026 T3770 T3769 arg1Of vector,retroviral
R3027 T3770 T3771 arg1Of vector,(
R3028 T3770 T3774 arg1Of vector,lacking
R3029 T3772 T3771 arg2Of pMIP,(
R3030 T3773 T3771 arg3Of ),(
R3031 T3777 T3774 arg2Of insert,lacking
R3032 T3777 T3775 arg1Of insert,a
R3033 T3777 T3776 arg1Of insert,cDNA
R3034 T3777 T3778 arg1Of insert,(
R3035 T3780 T3778 arg2Of vector,(
R3036 T3780 T3779 arg1Of vector,empty
R3037 T3781 T3778 arg3Of ),(
R3629 T4639 T4640 arg1Of HOIP,is
R3630 T4639 T4641 arg2Of HOIP,required
R3631 T4641 T4640 arg2Of required,is
R3632 T4641 T4642 arg1Of required,for
R3633 T4645 T4643 arg1Of upregulation,CD40-mediated
R3634 T4645 T4644 arg1Of upregulation,CD80
R3635 T4645 T4646 arg1Of upregulation,and
R3636 T4646 T4642 arg2Of and,for
R3637 T4647 T4646 arg2Of activation,and
R3638 T4647 T4648 arg1Of activation,of
R3639 T4651 T4648 arg2Of transcription,of
R3640 T4651 T4649 arg1Of transcription,germline
R3641 T4651 T4650 arg1Of transcription,epsilon
R3642 T4652 T4653 arg1Of Engagement,of
R3643 T4652 T4655 arg1Of Engagement,on
R3644 T4652 T4658 arg1Of Engagement,upregulates
R3645 T4654 T4653 arg2Of CD40,of
R3646 T4657 T4655 arg2Of cells,on
R3647 T4657 T4656 arg1Of cells,B
R3648 T4659 T4658 arg2Of expression,upregulates
R3649 T4659 T4660 arg1Of expression,of
R3650 T4661 T4660 arg2Of CD80,of
R3651 T4661 T4662 arg1Of CD80,[
R3652 T4661 T4665 arg1Of CD80,","
R3653 T4663 T4662 arg2Of 10,[
R3654 T4664 T4662 arg3Of ],[
R3655 T4669 T4665 arg2Of protein,","
R3656 T4669 T4666 arg1Of protein,a
R3657 T4669 T4667 arg1Of protein,cell
R3658 T4669 T4668 arg1Of protein,surface
R3659 T4669 T4670 arg1Of protein,that
R3660 T4669 T4671 arg1Of protein,promotes
R3661 T4672 T4673 arg1Of activation,of
R3662 T4672 T4680 arg1Of activation,and
R3663 T4675 T4673 arg2Of cells,of
R3664 T4675 T4674 arg1Of cells,T
R3665 T4675 T4676 arg1Of cells,interacting
R3666 T4676 T4677 arg1Of interacting,with
R3667 T4679 T4677 arg2Of cells,with
R3668 T4679 T4678 arg1Of cells,B
R3669 T4680 T4671 arg2Of and,promotes
R3670 T4682 T4680 arg2Of APC,and
R3671 T4682 T4681 arg1Of APC,other
R3672 T4684 T4683 arg1Of begin,To
R3673 T4685 T4684 arg2Of evaluating,begin
R3674 T4687 T4685 arg2Of contributions,evaluating
R3675 T4687 T4686 arg1Of contributions,potential
R3676 T4687 T4688 arg1Of contributions,of
R3677 T4689 T4688 arg2Of HOIP,of
R3678 T4689 T4690 arg1Of HOIP,to
R3679 T4692 T4690 arg2Of signaling,to
R3680 T4692 T4691 arg1Of signaling,CD40
R3681 T4694 T4684 arg1Of we,begin
R3682 T4694 T4685 arg1Of we,evaluating
R3683 T4694 T4695 arg1Of we,assayed
R3684 T4695 T4683 modOf assayed,To
R3685 T4695 T4693 arg1Of assayed,","
R3686 T4697 T4695 arg2Of upregulation,assayed
R3687 T4697 T4696 arg1Of upregulation,the
R3688 T4697 T4698 arg1Of upregulation,of
R3689 T4699 T4698 arg2Of CD80,of
R3690 T4699 T4700 arg2Of CD80,induced
R3691 T4702 T4700 arg1Of engagement,induced
R3692 T4702 T4701 arg2Of engagement,by
R3693 T4702 T4703 arg1Of engagement,of
R3694 T4704 T4703 arg2Of CD40,of
R3695 T4704 T4705 arg1Of CD40,on
R3696 T4707 T4708 arg1Of A20.2J,","
R3697 T4709 T4711 arg1Of HOIP-deficient,and
R3698 T4711 T4708 arg2Of and,","
R3699 T4711 T4710 arg1Of and,","
R3700 T4712 T4711 arg2Of HOIP-reconstituted,and
R3701 T4713 T4705 arg2Of cells,on
R3702 T4713 T4706 arg1Of cells,parental
R3703 T4713 T4707 arg1Of cells,A20.2J
R3704 T4713 T4709 arg1Of cells,HOIP-deficient
R3705 T4713 T4712 arg1Of cells,HOIP-reconstituted
R3706 T4713 T4714 arg1Of cells,(
R3707 T4715 T4714 arg2Of Fig.,(
R3708 T4715 T4716 arg1Of Fig.,2
R3709 T4717 T4714 arg3Of ),(
R3710 T4719 T4718 arg2Of to,Compared
R3711 T4723 T4719 arg2Of line,to
R3712 T4723 T4720 arg1Of line,the
R3713 T4723 T4721 arg1Of line,parental
R3714 T4723 T4722 arg1Of line,cell
R3715 T4727 T4725 arg1Of upregulation,the
R3716 T4727 T4726 arg1Of upregulation,CD40-stimulated
R3717 T4727 T4728 arg1Of upregulation,of
R3718 T4727 T4730 arg1Of upregulation,was
R3719 T4727 T4732 arg2Of upregulation,reduced
R3720 T4729 T4728 arg2Of CD80,of
R3721 T4732 T4718 arg1Of reduced,Compared
R3722 T4732 T4724 arg1Of reduced,","
R3723 T4732 T4730 arg2Of reduced,was
R3724 T4732 T4731 arg1Of reduced,dramatically
R3725 T4732 T4733 arg1Of reduced,in
R3726 T4736 T4733 arg2Of cells,in
R3727 T4736 T4734 arg1Of cells,all
R3728 T4736 T4735 arg1Of cells,HOIP-deficient
R3729 T4736 T4737 arg2Of cells,tested
R3730 T4739 T4738 arg2Of contrast,In
R3731 T4741 T4742 arg1Of upregulation,of
R3732 T4741 T4744 arg1Of upregulation,was
R3733 T4741 T4745 arg2Of upregulation,restored
R3734 T4743 T4742 arg2Of CD80,of
R3735 T4745 T4738 arg1Of restored,In
R3736 T4745 T4740 arg1Of restored,","
R3737 T4745 T4744 arg2Of restored,was
R3738 T4745 T4746 arg1Of restored,in
R3739 T4745 T4749 arg1Of restored,","
R3740 T4745 T4750 modOf restored,confirming
R3741 T4748 T4746 arg2Of cells,in
R3742 T4748 T4747 arg1Of cells,HOIP-reconstituted
R3743 T4753 T4752 arg1Of defect,the
R3744 T4753 T4754 arg1Of defect,in
R3745 T4753 T4757 arg1Of defect,in
R3746 T4753 T4761 arg1Of defect,was
R3747 T4753 T4762 arg1Of defect,due
R3748 T4756 T4754 arg2Of upregulation,in
R3749 T4756 T4755 arg1Of upregulation,CD80
R3750 T4760 T4757 arg2Of cells,in
R3751 T4760 T4758 arg1Of cells,the
R3752 T4760 T4759 arg1Of cells,HOIP-deficient
R3753 T4761 T4750 arg2Of was,confirming
R3754 T4761 T4751 arg1Of was,that
R3755 T4762 T4761 arg2Of due,was
R3756 T4762 T4763 arg1Of due,to
R3757 T4765 T4763 arg2Of disruption,to
R3758 T4765 T4764 arg1Of disruption,the
R3759 T4765 T4766 arg1Of disruption,of
R3760 T4767 T4766 arg2Of Rnf31,of
R3761 T4769 T4768 arg1Of signals,CD40
R3762 T4769 T4770 arg1Of signals,in
R3763 T4769 T4773 arg1Of signals,can
R3764 T4769 T4775 arg1Of signals,contribute
R3765 T4772 T4770 arg2Of cells,in
R3766 T4772 T4771 arg1Of cells,B
R3767 T4775 T4773 arg2Of contribute,can
R3768 T4775 T4774 arg1Of contribute,also
R3769 T4775 T4776 arg1Of contribute,to
R3770 T4775 T4791 arg1Of contribute,","
R3771 T4775 T4792 arg1Of contribute,[
R3772 T4778 T4776 arg2Of activation,to
R3773 T4778 T4777 arg1Of activation,the
R3774 T4778 T4779 arg1Of activation,of
R3775 T4778 T4782 arg1Of activation,in
R3776 T4781 T4779 arg2Of recombination,of
R3777 T4781 T4780 arg1Of recombination,DNA
R3778 T4787 T4782 arg2Of locus,in
R3779 T4787 T4783 arg1Of locus,the
R3780 T4787 T4784 arg1Of locus,immunoglobulin
R3781 T4787 T4785 arg1Of locus,heavy
R3782 T4787 T4786 arg1Of locus,chain
R3783 T4787 T4788 arg1Of locus,[
R3784 T4789 T4788 arg2Of 11,[
R3785 T4790 T4788 arg3Of ],[
R3786 T4793 T4792 arg2Of 12,[
R3787 T4794 T4792 arg3Of ],[
R3788 T4796 T4795 arg1Of process,This
R3789 T4796 T4797 arg1Of process,","
R3790 T4796 T4798 arg2Of process,known
R3791 T4796 T4809 arg1Of process,allows
R3792 T4796 T4814 arg1Of process,expressing
R3793 T4798 T4799 arg1Of known,as
R3794 T4802 T4799 arg2Of switching,as
R3795 T4802 T4800 arg1Of switching,immunoglobulin
R3796 T4802 T4801 arg1Of switching,class
R3797 T4802 T4803 arg1Of switching,(
R3798 T4806 T4803 arg2Of switching,(
R3799 T4806 T4804 arg1Of switching,or
R3800 T4806 T4805 arg1Of switching,isotype
R3801 T4807 T4803 arg3Of ),(
R3802 T4809 T4808 arg1Of allows,","
R3803 T4809 T4812 arg1Of allows,","
R3804 T4809 T4814 modOf allows,expressing
R3805 T4811 T4809 arg2Of cells,allows
R3806 T4811 T4810 arg1Of cells,B
R3807 T4814 T4813 arg1Of expressing,initially
R3808 T4814 T4817 modOf expressing,to
R3809 T4815 T4814 arg2Of IgM,expressing
R3810 T4815 T4833 arg1Of IgM,appropriate
R3811 T4818 T4816 arg1Of switch,","
R3812 T4818 T4817 arg1Of switch,to
R3813 T4818 T4819 arg1Of switch,to
R3814 T4818 T4826 arg1Of switch,depending
R3815 T4820 T4821 arg1Of IgA,","
R3816 T4821 T4824 arg1Of ",",or
R3817 T4822 T4821 arg2Of IgG,","
R3818 T4824 T4819 arg2Of or,to
R3819 T4824 T4823 arg1Of or,","
R3820 T4825 T4824 arg2Of IgE,or
R3821 T4827 T4826 arg2Of on,depending
R3822 T4829 T4827 arg2Of class,on
R3823 T4829 T4828 arg1Of class,the
R3824 T4829 T4830 arg1Of class,of
R3825 T4831 T4830 arg2Of antibody,of
R3826 T4833 T4814 arg3Of appropriate,expressing
R3827 T4833 T4832 arg1Of appropriate,most
R3828 T4833 T4834 arg1Of appropriate,for
R3829 T4838 T4834 arg2Of response,for
R3830 T4838 T4835 arg1Of response,a
R3831 T4838 T4836 arg1Of response,particular
R3832 T4838 T4837 arg1Of response,immune
R3833 T4839 T4840 arg1Of Switching,to
R3834 T4839 T4842 arg1Of Switching,can
R3835 T4839 T4843 arg1Of Switching,be
R3836 T4839 T4844 arg2Of Switching,induced
R3837 T4841 T4840 arg2Of IgE,to
R3838 T4844 T4842 arg2Of induced,can
R3839 T4844 T4843 arg2Of induced,be
R3840 T4847 T4844 arg1Of combination,induced
R3841 T4847 T4845 arg2Of combination,by
R3842 T4847 T4846 arg1Of combination,the
R3843 T4847 T4848 arg1Of combination,of
R3844 T4849 T4850 arg1Of IL-4,and
R3845 T4851 T4850 arg2Of CD40,and
R3846 T4852 T4848 arg2Of signals,of
R3847 T4852 T4849 arg1Of signals,IL-4
R3848 T4852 T4851 arg1Of signals,CD40
R3849 T4855 T4853 arg1Of rearrangement,The
R3850 T4855 T4854 arg1Of rearrangement,gene
R3851 T4855 T4856 arg1Of rearrangement,necessary
R3852 T4855 T4860 arg1Of rearrangement,is
R3853 T4855 T4861 arg2Of rearrangement,preceded
R3854 T4856 T4857 arg1Of necessary,for
R3855 T4859 T4857 arg2Of production,for
R3856 T4859 T4858 arg1Of production,IgE
R3857 T4861 T4860 arg2Of preceded,is
R3858 T4864 T4861 arg1Of production,preceded
R3859 T4864 T4862 arg2Of production,by
R3860 T4864 T4863 arg1Of production,the
R3861 T4864 T4865 arg1Of production,of
R3862 T4868 T4865 arg2Of transcripts,of
R3863 T4868 T4866 arg1Of transcripts,non-coding
R3864 T4868 T4867 arg1Of transcripts,RNA
R3865 T4868 T4869 arg1Of transcripts,from
R3866 T4871 T4869 arg2Of sequences,from
R3867 T4871 T4870 arg1Of sequences,germline
R3868 T4871 T4872 arg1Of sequences,in
R3869 T4877 T4872 arg2Of locus,in
R3870 T4877 T4873 arg1Of locus,the
R3871 T4877 T4874 arg1Of locus,IgE
R3872 T4877 T4875 arg1Of locus,heavy
R3873 T4877 T4876 arg1Of locus,chain
R3874 T4877 T4878 arg1Of locus,[
R3875 T4879 T4878 arg2Of 13,[
R3876 T4880 T4878 arg3Of ],[
R3877 T4882 T4881 arg1Of determine,To
R3878 T4884 T4885 arg1Of HOIP,is
R3879 T4884 T4886 arg2Of HOIP,required
R3880 T4886 T4882 arg2Of required,determine
R3881 T4886 T4883 arg1Of required,whether
R3882 T4886 T4885 arg2Of required,is
R3883 T4886 T4887 arg1Of required,in
R3884 T4889 T4887 arg2Of initiation,in
R3885 T4889 T4888 arg1Of initiation,the
R3886 T4889 T4890 arg1Of initiation,of
R3887 T4894 T4890 arg2Of process,of
R3888 T4894 T4891 arg1Of process,the
R3889 T4894 T4892 arg1Of process,class
R3890 T4894 T4893 arg1Of process,switching
R3891 T4900 T4896 arg1Of lines,the
R3892 T4900 T4897 arg1Of lines,relevant
R3893 T4900 T4898 arg1Of lines,B
R3894 T4900 T4899 arg1Of lines,cell
R3895 T4900 T4901 arg1Of lines,were
R3896 T4900 T4902 arg2Of lines,stimulated
R3897 T4900 T4911 arg2Of lines,tested
R3898 T4902 T4903 arg1Of stimulated,through
R3899 T4902 T4905 arg1Of stimulated,in
R3900 T4902 T4910 arg1Of stimulated,and
R3901 T4904 T4903 arg2Of CD40,through
R3902 T4907 T4905 arg2Of presence,in
R3903 T4907 T4906 arg1Of presence,the
R3904 T4907 T4908 arg1Of presence,of
R3905 T4909 T4908 arg2Of IL-4,of
R3906 T4910 T4881 modOf and,To
R3907 T4910 T4895 arg1Of and,","
R3908 T4910 T4901 arg2Of and,were
R3909 T4911 T4910 arg2Of tested,and
R3910 T4911 T4912 arg1Of tested,for
R3911 T4913 T4912 arg2Of production,for
R3912 T4913 T4914 arg1Of production,of
R3913 T4916 T4915 arg1Of epsilon,germline
R3914 T4916 T4917 arg1Of epsilon,(
R3915 T4918 T4917 arg2Of GLε,(
R3916 T4919 T4917 arg3Of ),(
R3917 T4920 T4914 arg2Of transcripts,of
R3918 T4920 T4916 arg1Of transcripts,epsilon
R3919 T4920 T4921 arg1Of transcripts,(
R3920 T4922 T4921 arg2Of Fig.,(
R3921 T4922 T4923 arg1Of Fig.,3
R3922 T4924 T4921 arg3Of ),(
R3923 T4927 T4925 arg1Of stimulation,Anti-CD40
R3924 T4927 T4926 arg1Of stimulation,antibody
R3925 T4927 T4928 arg1Of stimulation,alone
R3926 T4927 T4930 arg1Of stimulation,together
R3927 T4927 T4933 arg1Of stimulation,induced
R3928 T4928 T4929 arg1Of alone,or
R3929 T4929 T4931 arg1Of or,with
R3930 T4930 T4929 arg2Of together,or
R3931 T4932 T4931 arg2Of IL-4,with
R3932 T4935 T4933 arg2Of transcription,induced
R3933 T4935 T4934 arg1Of transcription,GLε
R3934 T4935 T4936 arg1Of transcription,in
R3935 T4939 T4936 arg2Of cells,in
R3936 T4939 T4937 arg1Of cells,parental
R3937 T4939 T4938 arg1Of cells,A20.2J
R3938 T4940 T4933 arg3Of as,induced
R3939 T4941 T4940 arg2Of expected,as
R3940 T4943 T4942 arg2Of contrast,In
R3941 T4947 T4945 arg1Of levels,only
R3942 T4947 T4946 arg1Of levels,low
R3943 T4947 T4948 arg1Of levels,of
R3944 T4947 T4951 arg1Of levels,were
R3945 T4947 T4952 arg2Of levels,detected
R3946 T4950 T4948 arg2Of expression,of
R3947 T4950 T4949 arg1Of expression,GLε
R3948 T4952 T4942 arg1Of detected,In
R3949 T4952 T4944 arg1Of detected,","
R3950 T4952 T4951 arg2Of detected,were
R3951 T4952 T4953 arg1Of detected,in
R3952 T4955 T4953 arg2Of cells,in
R3953 T4955 T4954 arg1Of cells,HOIP-deficient
R3954 T4955 T4956 arg2Of cells,stimulated
R3955 T4956 T4957 arg1Of stimulated,with
R3956 T4960 T4959 arg1Of antibody,anti-CD40
R3957 T4960 T4961 arg1Of antibody,alone
R3958 T4960 T4962 arg1Of antibody,or
R3959 T4962 T4957 arg2Of or,with
R3960 T4962 T4958 arg1Of or,either
R3961 T4963 T4964 arg1Of anti-CD40,and
R3962 T4964 T4962 arg2Of and,or
R3963 T4965 T4964 arg2Of IL-4,and
R3964 T4967 T4966 arg1Of levels,Normal
R3965 T4967 T4968 arg1Of levels,of
R3966 T4967 T4971 arg1Of levels,were
R3967 T4967 T4972 arg2Of levels,induced
R3968 T4970 T4968 arg2Of expression,of
R3969 T4970 T4969 arg1Of expression,GLε
R3970 T4972 T4971 arg2Of induced,were
R3971 T4972 T4973 arg1Of induced,in
R3972 T4975 T4973 arg2Of cells,in
R3973 T4975 T4974 arg1Of cells,HOIP-reconstituted
R3974 T4975 T4976 arg2Of cells,stimulated
R3975 T4976 T4977 arg1Of stimulated,with
R3976 T4979 T4977 arg2Of antibody,with
R3977 T4979 T4978 arg1Of antibody,anti-CD40
R3978 T4979 T4980 arg1Of antibody,alone
R3979 T4979 T4982 arg1Of antibody,together
R3980 T4980 T4981 arg1Of alone,or
R3981 T4981 T4983 arg1Of or,with
R3982 T4982 T4981 arg2Of together,or
R3983 T4984 T4983 arg2Of IL-4,with
R3984 T4988 T4987 arg1Of data,these
R3985 T4988 T4989 arg1Of data,demonstrate
R3986 T4989 T4985 arg1Of demonstrate,Collectively
R3987 T4989 T4986 arg1Of demonstrate,","
R3988 T4991 T4992 arg1Of HOIP,has
R3989 T4992 T4989 arg2Of has,demonstrate
R3990 T4992 T4990 arg1Of has,that
R3991 T4992 T4996 arg1Of has,in
R3992 T4995 T4992 arg2Of role,has
R3993 T4995 T4993 arg1Of role,a
R3994 T4995 T4994 arg1Of role,critical
R3995 T4998 T4996 arg2Of responses,in
R3996 T4998 T4997 arg1Of responses,cellular
R3997 T4998 T4999 arg1Of responses,to
R3998 T5001 T4999 arg2Of signaling,to
R3999 T5001 T5000 arg1Of signaling,CD40
R4483 T5789 T5790 arg1Of HOIP,mediates
R4484 T5792 T5791 arg1Of NF-κB,CD40-stimulated
R4485 T5792 T5793 arg1Of NF-κB,and
R4486 T5793 T5790 arg2Of and,mediates
R4487 T5795 T5793 arg2Of activation,and
R4488 T5795 T5794 arg1Of activation,JNK
R4489 T5797 T5796 arg1Of results,The
R4490 T5797 T5798 arg2Of results,described
R4491 T5797 T5800 arg1Of results,show
R4492 T5798 T5799 arg1Of described,above
R4493 T5802 T5803 arg1Of HOIP,plays
R4494 T5803 T5800 arg2Of plays,show
R4495 T5803 T5801 arg1Of plays,that
R4496 T5803 T5807 arg1Of plays,in
R4497 T5806 T5803 arg2Of role,plays
R4498 T5806 T5804 arg1Of role,an
R4499 T5806 T5805 arg1Of role,important
R4500 T5810 T5807 arg2Of functions,in
R4501 T5810 T5808 arg1Of functions,CD40-mediated
R4502 T5810 T5809 arg1Of functions,effector
R4503 T5810 T5811 arg1Of functions,of
R4504 T5813 T5811 arg2Of cells,of
R4505 T5813 T5812 arg1Of cells,B
R4506 T5817 T5818 arg1Of HOIP,is
R4507 T5818 T5814 arg1Of is,It
R4508 T5818 T5815 arg1Of is,follows
R4509 T5818 T5816 arg1Of is,that
R4510 T5818 T5819 arg1Of is,likely
R4511 T5822 T5818 arg2Of mediator,is
R4512 T5822 T5820 arg1Of mediator,a
R4513 T5822 T5821 arg1Of mediator,key
R4514 T5822 T5823 arg1Of mediator,of
R4515 T5825 T5823 arg2Of signaling,of
R4516 T5825 T5824 arg1Of signaling,CD40
R4517 T5827 T5826 arg1Of test,To
R4518 T5829 T5827 arg2Of hypothesis,test
R4519 T5829 T5828 arg1Of hypothesis,this
R4520 T5831 T5827 arg1Of we,test
R4521 T5831 T5832 arg1Of we,stimulated
R4522 T5832 T5826 modOf stimulated,To
R4523 T5832 T5830 arg1Of stimulated,","
R4524 T5833 T5834 arg1Of A20.2J,and
R4525 T5834 T5832 arg2Of and,stimulated
R4526 T5834 T5837 arg1Of and,with
R4527 T5836 T5834 arg2Of cells,and
R4528 T5836 T5835 arg1Of cells,HOIP-deficient
R4529 T5838 T5839 arg1Of CD154,(
R4530 T5838 T5843 arg2Of CD154,expressed
R4531 T5838 T5855 arg1Of CD154,and
R4532 T5841 T5839 arg2Of ligand,(
R4533 T5841 T5840 arg1Of ligand,CD40
R4534 T5842 T5839 arg3Of ),(
R4535 T5843 T5851 arg1Of expressed,","
R4536 T5843 T5852 arg1Of expressed,[
R4537 T5847 T5843 arg1Of cells,expressed
R4538 T5847 T5844 arg2Of cells,by
R4539 T5847 T5845 arg1Of cells,HI5
R4540 T5847 T5846 arg1Of cells,insect
R4541 T5847 T5848 arg1Of cells,[
R4542 T5849 T5848 arg2Of 7,[
R4543 T5850 T5848 arg3Of ],[
R4544 T5853 T5852 arg2Of 8,[
R4545 T5854 T5852 arg3Of ],[
R4546 T5855 T5837 arg2Of and,with
R4547 T5857 T5855 arg2Of activation,and
R4548 T5857 T5856 arg2Of activation,measured
R4549 T5857 T5858 arg1Of activation,of
R4550 T5860 T5861 arg1Of NF-κB,and
R4551 T5862 T5861 arg2Of JNK,and
R4552 T5863 T5858 arg2Of pathways,of
R4553 T5863 T5859 arg1Of pathways,the
R4554 T5863 T5860 arg1Of pathways,NF-κB
R4555 T5863 T5862 arg1Of pathways,JNK
R4556 T5863 T5864 arg1Of pathways,","
R4557 T5865 T5864 arg2Of two,","
R4558 T5865 T5866 arg1Of two,of
R4559 T5870 T5866 arg2Of regulators,of
R4560 T5870 T5867 arg1Of regulators,the
R4561 T5870 T5868 arg1Of regulators,major
R4562 T5870 T5869 arg1Of regulators,transcriptional
R4563 T5870 T5871 arg2Of regulators,activated
R4564 T5871 T5874 arg1Of activated,[
R4565 T5873 T5871 arg1Of CD40,activated
R4566 T5873 T5872 arg2Of CD40,by
R4567 T5875 T5874 arg2Of 4,[
R4568 T5876 T5874 arg3Of ],[
R4569 T5878 T5877 arg1Of CD154,Cell-associated
R4570 T5878 T5879 arg1Of CD154,was
R4571 T5878 T5880 arg2Of CD154,used
R4572 T5880 T5879 arg2Of used,was
R4573 T5880 T5881 arg1Of used,as
R4574 T5883 T5881 arg2Of stimulus,as
R4575 T5883 T5882 arg1Of stimulus,the
R4576 T5883 T5884 arg1Of stimulus,in
R4577 T5886 T5884 arg2Of experiments,in
R4578 T5886 T5885 arg1Of experiments,these
R4579 T5886 T5887 arg1Of experiments,as
R4580 T5888 T5890 arg1Of it,provides
R4581 T5890 T5887 arg2Of provides,as
R4582 T5890 T5889 arg1Of provides,typically
R4583 T5892 T5891 arg1Of robust,more
R4584 T5892 T5894 arg1Of robust,and
R4585 T5894 T5893 arg1Of and,","
R4586 T5896 T5894 arg2Of more,and
R4587 T5896 T5895 arg1Of more,therefore
R4588 T5898 T5897 arg1Of detected,readily
R4589 T5901 T5890 arg2Of signals,provides
R4590 T5901 T5892 arg1Of signals,robust
R4591 T5901 T5896 arg1Of signals,more
R4592 T5901 T5898 arg2Of signals,detected
R4593 T5901 T5899 arg1Of signals,","
R4594 T5901 T5900 arg1Of signals,activation
R4595 T5901 T5902 arg1Of signals,than
R4596 T5903 T5902 arg2Of does,than
R4597 T5905 T5903 arg1Of antibody,does
R4598 T5905 T5904 arg1Of antibody,anti-CD40
R4599 T5906 T5907 arg1Of Activation,of
R4600 T5906 T5912 arg1Of Activation,is
R4601 T5906 T5913 arg2Of Activation,initiated
R4602 T5911 T5907 arg2Of pathway,of
R4603 T5911 T5908 arg1Of pathway,the
R4604 T5911 T5909 arg1Of pathway,canonical
R4605 T5911 T5910 arg1Of pathway,NF-κB
R4606 T5913 T5912 arg2Of initiated,is
R4607 T5913 T5914 arg1Of initiated,with
R4608 T5916 T5914 arg2Of phosphorylation,with
R4609 T5916 T5915 arg1Of phosphorylation,the
R4610 T5916 T5917 arg1Of phosphorylation,of
R4611 T5916 T5920 arg1Of phosphorylation,by
R4612 T5919 T5917 arg2Of proteins,of
R4613 T5919 T5918 arg1Of proteins,IκB
R4614 T5924 T5920 arg2Of complex,by
R4615 T5924 T5921 arg1Of complex,the
R4616 T5924 T5922 arg1Of complex,IκB
R4617 T5924 T5923 arg1Of complex,kinase
R4618 T5924 T5925 arg1Of complex,(
R4619 T5926 T5925 arg2Of IKK,(
R4620 T5927 T5925 arg3Of ),(
R4621 T5930 T5928 arg2Of cells,In
R4622 T5930 T5929 arg1Of cells,resting
R4623 T5933 T5932 arg1Of proteins,IκB
R4624 T5933 T5934 arg1Of proteins,are
R4625 T5933 T5935 arg1Of proteins,responsible
R4626 T5934 T5928 arg1Of are,In
R4627 T5934 T5931 arg1Of are,","
R4628 T5935 T5934 arg2Of responsible,are
R4629 T5935 T5936 arg1Of responsible,for
R4630 T5937 T5936 arg2Of sequestering,for
R4631 T5937 T5940 arg1Of sequestering,in
R4632 T5939 T5937 arg2Of subunits,sequestering
R4633 T5939 T5938 arg1Of subunits,NF-κB
R4634 T5942 T5940 arg2Of cytoplasm,in
R4635 T5942 T5941 arg1Of cytoplasm,the
R4636 T5943 T5944 arg1Of Phosphorylation,by
R4637 T5943 T5948 arg1Of Phosphorylation,targets
R4638 T5947 T5944 arg2Of complex,by
R4639 T5947 T5945 arg1Of complex,the
R4640 T5947 T5946 arg1Of complex,IKK
R4641 T5948 T5955 arg1Of targets,","
R4642 T5948 T5956 modOf targets,allowing
R4643 T5950 T5948 arg2Of proteins,targets
R4644 T5950 T5949 arg1Of proteins,IκB
R4645 T5950 T5951 arg1Of proteins,for
R4646 T5952 T5953 arg1Of ubiquitination,and
R4647 T5953 T5951 arg2Of and,for
R4648 T5954 T5953 arg2Of degradation,and
R4649 T5957 T5956 arg2Of NF-κB,allowing
R4650 T5957 T5959 arg1Of NF-κB,enter
R4651 T5957 T5963 arg1Of NF-κB,activate
R4652 T5959 T5962 arg1Of enter,and
R4653 T5961 T5959 arg2Of nucleus,enter
R4654 T5961 T5960 arg1Of nucleus,the
R4655 T5962 T5956 arg3Of and,allowing
R4656 T5962 T5958 arg1Of and,to
R4657 T5963 T5962 arg2Of activate,and
R4658 T5965 T5963 arg2Of expression,activate
R4659 T5965 T5964 arg1Of expression,gene
R4660 T5967 T5966 arg1Of phosphorylation,CD40-mediated
R4661 T5967 T5968 arg1Of phosphorylation,and
R4662 T5968 T5972 arg1Of and,in
R4663 T5968 T5975 arg1Of and,was
R4664 T5968 T5977 arg1Of and,impaired
R4665 T5969 T5968 arg2Of degradation,and
R4666 T5969 T5970 arg1Of degradation,of
R4667 T5971 T5970 arg2Of IκBα,of
R4668 T5974 T5972 arg2Of cells,in
R4669 T5974 T5973 arg1Of cells,HOIP-deficient
R4670 T5977 T5975 arg2Of impaired,was
R4671 T5977 T5976 arg1Of impaired,dramatically
R4672 T5977 T5979 arg1Of impaired,to
R4673 T5979 T5978 arg1Of to,relative
R4674 T5980 T5979 arg2Of that,to
R4675 T5980 T5981 arg2Of that,observed
R4676 T5981 T5982 arg1Of observed,in
R4677 T5985 T5982 arg2Of cells,in
R4678 T5985 T5983 arg1Of cells,parental
R4679 T5985 T5984 arg1Of cells,A20.2J
R4680 T5985 T5986 arg1Of cells,(
R4681 T5987 T5986 arg2Of Fig.,(
R4682 T5987 T5988 arg1Of Fig.,4
R4683 T5989 T5986 arg3Of ),(
R4684 T5990 T5992 arg1Of We,assayed
R4685 T5992 T5991 arg1Of assayed,also
R4686 T5992 T6000 arg1Of assayed,in
R4687 T5993 T5992 arg2Of activation,assayed
R4688 T5993 T5994 arg1Of activation,of
R4689 T5999 T5994 arg2Of JNK,of
R4690 T5999 T5995 arg1Of JNK,the
R4691 T5999 T5996 arg1Of JNK,stress-activated
R4692 T5999 T5997 arg1Of JNK,protein
R4693 T5999 T5998 arg1Of JNK,kinase
R4694 T6001 T6000 arg2Of response,in
R4695 T6001 T6002 arg1Of response,to
R4696 T6004 T6002 arg2Of engagement,to
R4697 T6004 T6003 arg1Of engagement,CD40
R4698 T6004 T6005 arg1Of engagement,(
R4699 T6006 T6005 arg2Of Fig.,(
R4700 T6006 T6007 arg1Of Fig.,4
R4701 T6008 T6005 arg3Of ),(
R4702 T6011 T6009 arg1Of activation,CD40-mediated
R4703 T6011 T6010 arg1Of activation,JNK
R4704 T6011 T6012 arg1Of activation,in
R4705 T6011 T6015 arg1Of activation,was
R4706 T6011 T6016 arg2Of activation,impaired
R4707 T6014 T6012 arg2Of cells,in
R4708 T6014 T6013 arg1Of cells,HOIP-deficient
R4709 T6016 T6015 arg2Of impaired,was
R4710 T6016 T6017 arg1Of impaired,as
R4711 T6018 T6017 arg2Of measured,as
R4712 T6020 T6018 arg1Of phosphorylation,measured
R4713 T6020 T6019 arg2Of phosphorylation,by
R4714 T6020 T6021 arg1Of phosphorylation,of
R4715 T6022 T6023 arg1Of Thr183,and
R4716 T6023 T6021 arg2Of and,of
R4717 T6023 T6025 arg1Of and,in
R4718 T6024 T6023 arg2Of Tyr185,and
R4719 T6026 T6025 arg2Of JNK,in
R4720 T6028 T6027 arg1Of activation,CD40-induced
R4721 T6028 T6029 arg1Of activation,of
R4722 T6028 T6033 arg1Of activation,in
R4723 T6028 T6036 arg1Of activation,was
R4724 T6028 T6037 arg1Of activation,normal
R4725 T6030 T6031 arg1Of NF-κB,and
R4726 T6031 T6029 arg2Of and,of
R4727 T6032 T6031 arg2Of JNK,and
R4728 T6035 T6033 arg2Of cells,in
R4729 T6035 T6034 arg1Of cells,HOIP-reconstituted
R4730 T6036 T6038 arg1Of was,","
R4731 T6036 T6039 modOf was,demonstrating
R4732 T6037 T6036 arg2Of normal,was
R4733 T6042 T6041 arg1Of defects,the
R4734 T6042 T6043 arg2Of defects,observed
R4735 T6042 T6047 arg1Of defects,were
R4736 T6042 T6048 arg1Of defects,due
R4737 T6043 T6044 arg1Of observed,in
R4738 T6046 T6044 arg2Of cells,in
R4739 T6046 T6045 arg1Of cells,gene-deficient
R4740 T6047 T6039 arg2Of were,demonstrating
R4741 T6047 T6040 arg1Of were,that
R4742 T6048 T6047 arg2Of due,were
R4743 T6048 T6049 arg1Of due,to
R4744 T6051 T6049 arg2Of absence,to
R4745 T6051 T6050 arg1Of absence,the
R4746 T6051 T6052 arg1Of absence,of
R4747 T6054 T6052 arg2Of expression,of
R4748 T6054 T6053 arg1Of expression,HOIP
R5843 T7485 T7484 arg2Of CD40,with
R5844 T7488 T7486 arg1Of defects,The
R5845 T7488 T7487 arg1Of defects,marked
R5846 T7488 T7489 arg1Of defects,in
R5847 T7488 T7495 arg2Of defects,displayed
R5848 T7488 T7499 arg1Of defects,suggested
R5849 T7492 T7493 arg1Of activation,and
R5850 T7493 T7489 arg2Of and,in
R5851 T7493 T7490 arg1Of and,CD40-mediated
R5852 T7493 T7491 arg1Of and,cell
R5853 T7494 T7493 arg2Of signaling,and
R5854 T7498 T7495 arg1Of cells,displayed
R5855 T7498 T7496 arg2Of cells,by
R5856 T7498 T7497 arg1Of cells,HOIP-deficient
R5857 T7501 T7502 arg1Of HOIP,mediates
R5858 T7502 T7499 arg2Of mediates,suggested
R5859 T7502 T7500 arg1Of mediates,that
R5860 T7503 T7502 arg2Of recruitment,mediates
R5861 T7503 T7504 arg1Of recruitment,of
R5862 T7503 T7512 arg1Of recruitment,to
R5863 T7506 T7504 arg2Of components,of
R5864 T7506 T7505 arg1Of components,critical
R5865 T7506 T7507 arg1Of components,of
R5866 T7511 T7507 arg2Of apparatus,of
R5867 T7511 T7508 arg1Of apparatus,the
R5868 T7511 T7509 arg1Of apparatus,CD40
R5869 T7511 T7510 arg1Of apparatus,signaling
R5870 T7514 T7512 arg2Of receptor,to
R5871 T7514 T7513 arg1Of receptor,the
R5872 T7517 T7518 arg1Of we,demonstrated
R5873 T7518 T7515 arg1Of demonstrated,Previously
R5874 T7518 T7516 arg1Of demonstrated,","
R5875 T7520 T7521 arg1Of HOIP,is
R5876 T7520 T7522 arg2Of HOIP,recruited
R5877 T7522 T7518 arg2Of recruited,demonstrated
R5878 T7522 T7519 arg1Of recruited,that
R5879 T7522 T7521 arg2Of recruited,is
R5880 T7522 T7523 arg1Of recruited,to
R5881 T7522 T7528 arg1Of recruited,in
R5882 T7522 T7532 arg1Of recruited,","
R5883 T7522 T7533 modOf recruited,suggesting
R5884 T7522 T7540 arg1Of recruited,[
R5885 T7527 T7523 arg2Of complex,to
R5886 T7527 T7524 arg1Of complex,the
R5887 T7527 T7525 arg1Of complex,CD40
R5888 T7527 T7526 arg1Of complex,signaling
R5889 T7531 T7528 arg2Of manner,in
R5890 T7531 T7529 arg1Of manner,a
R5891 T7531 T7530 arg1Of manner,TRAF2-dependent
R5892 T7536 T7533 arg2Of functions,suggesting
R5893 T7536 T7534 arg1Of functions,that
R5894 T7536 T7535 arg1Of functions,HOIP
R5895 T7536 T7537 arg1Of functions,downstream
R5896 T7537 T7538 arg1Of downstream,of
R5897 T7539 T7538 arg2Of TRAF2,of
R5898 T7541 T7540 arg2Of 6,[
R5899 T7542 T7540 arg3Of ],[
R5900 T7543 T7544 arg1Of Studies,by
R5901 T7543 T7546 arg1Of Studies,suggest
R5902 T7545 T7544 arg2Of others,by
R5903 T7550 T7548 arg1Of proteins,the
R5904 T7550 T7549 arg1Of proteins,TRAF2-associated
R5905 T7551 T7552 arg1Of cIAP1,and
R5906 T7552 T7550 arg1Of and,proteins
R5907 T7552 T7554 arg1Of and,play
R5908 T7553 T7552 arg2Of cIAP2,and
R5909 T7554 T7546 arg2Of play,suggest
R5910 T7554 T7547 arg1Of play,that
R5911 T7554 T7557 arg1Of play,in
R5912 T7554 T7566 arg1Of play,[
R5913 T7556 T7554 arg2Of role,play
R5914 T7556 T7555 arg1Of role,a
R5915 T7559 T7557 arg2Of recruitment,in
R5916 T7559 T7558 arg1Of recruitment,the
R5917 T7559 T7560 arg1Of recruitment,of
R5918 T7559 T7562 arg1Of recruitment,to
R5919 T7561 T7560 arg2Of HOIP,of
R5920 T7563 T7564 arg1Of TNFR1,and
R5921 T7564 T7562 arg2Of and,to
R5922 T7565 T7564 arg2Of CD40,and
R5923 T7567 T7566 arg2Of 14,[
R5924 T7568 T7566 arg3Of ],[
R5925 T7571 T7572 arg1Of we,determined
R5926 T7572 T7569 arg1Of determined,Therefore
R5927 T7572 T7570 arg1Of determined,","
R5928 T7575 T7574 arg1Of association,the
R5929 T7575 T7576 arg1Of association,of
R5930 T7575 T7578 arg1Of association,with
R5931 T7575 T7580 arg1Of association,in
R5932 T7575 T7583 arg1Of association,was
R5933 T7575 T7584 arg2Of association,altered
R5934 T7577 T7576 arg2Of HOIP,of
R5935 T7579 T7578 arg2Of CD40,with
R5936 T7582 T7580 arg2Of cells,in
R5937 T7582 T7581 arg1Of cells,A20.2J
R5938 T7584 T7572 arg2Of altered,determined
R5939 T7584 T7573 arg1Of altered,whether
R5940 T7584 T7583 arg2Of altered,was
R5941 T7584 T7605 arg1Of altered,[
R5942 T7586 T7584 arg1Of treatment,altered
R5943 T7586 T7585 arg2Of treatment,by
R5944 T7586 T7587 arg1Of treatment,of
R5945 T7586 T7589 arg1Of treatment,with
R5946 T7586 T7599 arg2Of treatment,derived
R5947 T7588 T7587 arg2Of cells,of
R5948 T7591 T7589 arg2Of inhibitor,with
R5949 T7591 T7590 arg1Of inhibitor,an
R5950 T7591 T7592 arg1Of inhibitor,of
R5951 T7591 T7595 arg1Of inhibitor,","
R5952 T7594 T7592 arg2Of activity,of
R5953 T7594 T7593 arg1Of activity,cIAP
R5954 T7598 T7595 arg2Of peptide,","
R5955 T7598 T7596 arg1Of peptide,a
R5956 T7598 T7597 arg1Of peptide,membrane-permeable
R5957 T7599 T7600 arg1Of derived,from
R5958 T7604 T7600 arg2Of SMAC,from
R5959 T7604 T7601 arg1Of SMAC,the
R5960 T7604 T7602 arg1Of SMAC,apoptosis
R5961 T7604 T7603 arg1Of SMAC,regulator
R5962 T7606 T7605 arg2Of 15,[
R5963 T7607 T7605 arg3Of ],[
R5964 T7608 T7609 arg1Of We,found
R5965 T7611 T7612 arg1Of pretreatment,of
R5966 T7611 T7614 arg1Of pretreatment,with
R5967 T7611 T7619 arg1Of pretreatment,reduced
R5968 T7613 T7612 arg2Of cells,of
R5969 T7617 T7614 arg2Of peptide,with
R5970 T7617 T7615 arg1Of peptide,the
R5971 T7617 T7616 arg1Of peptide,SMAC
R5972 T7619 T7609 arg2Of reduced,found
R5973 T7619 T7610 arg1Of reduced,that
R5974 T7619 T7618 arg1Of reduced,dramatically
R5975 T7621 T7619 arg2Of amount,reduced
R5976 T7621 T7620 arg1Of amount,the
R5977 T7621 T7622 arg1Of amount,of
R5978 T7621 T7624 arg2Of amount,associated
R5979 T7623 T7622 arg2Of cIAP1,of
R5980 T7624 T7625 arg1Of associated,with
R5981 T7629 T7625 arg2Of complex,with
R5982 T7629 T7626 arg1Of complex,the
R5983 T7629 T7627 arg1Of complex,CD40
R5984 T7629 T7628 arg1Of complex,signaling
R5985 T7629 T7630 arg1Of complex,in
R5986 T7631 T7630 arg2Of cells,in
R5987 T7631 T7632 arg2Of cells,stimulated
R5988 T7632 T7633 arg1Of stimulated,with
R5989 T7636 T7633 arg2Of beads,with
R5990 T7636 T7634 arg1Of beads,anti-CD40
R5991 T7636 T7635 arg1Of beads,antibody-coated
R5992 T7636 T7637 arg1Of beads,(
R5993 T7639 T7637 arg2Of 5A,(
R5994 T7639 T7638 arg1Of 5A,Fig.
R5995 T7640 T7637 arg3Of ),(
R5996 T7643 T7641 arg1Of treatment,SMAC
R5997 T7643 T7642 arg1Of treatment,peptide
R5998 T7643 T7645 arg1Of treatment,resulted
R5999 T7645 T7644 arg1Of resulted,also
R6000 T7645 T7646 arg1Of resulted,in
R6001 T7648 T7649 arg1Of slight,but
R6002 T7650 T7649 arg2Of reproducible,but
R6003 T7651 T7646 arg2Of decrease,in
R6004 T7651 T7647 arg1Of decrease,a
R6005 T7651 T7648 arg1Of decrease,slight
R6006 T7651 T7650 arg1Of decrease,reproducible
R6007 T7651 T7652 arg1Of decrease,in
R6008 T7651 T7664 arg1Of decrease,","
R6009 T7651 T7665 arg1Of decrease,along
R6010 T7654 T7652 arg2Of amount,in
R6011 T7654 T7653 arg1Of amount,the
R6012 T7654 T7655 arg1Of amount,of
R6013 T7659 T7655 arg2Of form,of
R6014 T7659 T7656 arg1Of form,the
R6015 T7659 T7657 arg1Of form,major
R6016 T7659 T7658 arg1Of form,HOIP
R6017 T7659 T7660 arg2Of form,recovered
R6018 T7663 T7660 arg1Of immunoprecipitation,recovered
R6019 T7663 T7661 arg2Of immunoprecipitation,by
R6020 T7663 T7662 arg1Of immunoprecipitation,CD40
R6021 T7665 T7666 arg1Of along,with
R6022 T7669 T7666 arg2Of increase,with
R6023 T7669 T7667 arg1Of increase,an
R6024 T7669 T7668 arg1Of increase,apparent
R6025 T7669 T7670 arg1Of increase,in
R6026 T7674 T7670 arg2Of species,in
R6027 T7674 T7671 arg1Of species,higher
R6028 T7674 T7672 arg1Of species,molecular
R6029 T7674 T7673 arg1Of species,weight
R6030 T7674 T7675 arg2Of species,recognized
R6031 T7678 T7675 arg1Of antibody,recognized
R6032 T7678 T7676 arg2Of antibody,by
R6033 T7678 T7677 arg1Of antibody,anti-HOIP
R6034 T7680 T7679 arg2Of contrast,In
R6035 T7682 T7683 arg1Of treatment,with
R6036 T7682 T7687 arg1Of treatment,did
R6037 T7682 T7689 arg1Of treatment,alter
R6038 T7686 T7683 arg2Of peptide,with
R6039 T7686 T7684 arg1Of peptide,the
R6040 T7686 T7685 arg1Of peptide,SMAC
R6041 T7689 T7679 arg1Of alter,In
R6042 T7689 T7681 arg1Of alter,","
R6043 T7689 T7687 arg2Of alter,did
R6044 T7689 T7688 arg1Of alter,not
R6045 T7691 T7692 arg1Of amount,or
R6046 T7692 T7689 arg2Of or,alter
R6047 T7692 T7690 arg1Of or,the
R6048 T7692 T7695 arg1Of or,of
R6049 T7692 T7697 arg1Of or,present
R6050 T7694 T7692 arg2Of weight,or
R6051 T7694 T7693 arg1Of weight,molecular
R6052 T7696 T7695 arg2Of HOIP,of
R6053 T7697 T7698 arg1Of present,in
R6054 T7700 T7698 arg2Of lysates,in
R6055 T7700 T7699 arg1Of lysates,cell
R6056 T7702 T7701 arg1Of data,These
R6057 T7702 T7703 arg1Of data,suggest
R6058 T7707 T7705 arg1Of treatment,SMAC
R6059 T7707 T7706 arg1Of treatment,peptide
R6060 T7707 T7709 arg1Of treatment,alters
R6061 T7709 T7703 arg2Of alters,suggest
R6062 T7709 T7704 arg1Of alters,that
R6063 T7709 T7708 arg1Of alters,specifically
R6064 T7711 T7710 arg1Of characteristics,the
R6065 T7711 T7712 arg1Of characteristics,of
R6066 T7711 T7716 arg1Of characteristics,than
R6067 T7714 T7712 arg2Of HOIP,of
R6068 T7714 T7713 arg1Of HOIP,CD40-associated
R6069 T7716 T7709 arg2Of than,alters
R6070 T7716 T7715 arg1Of than,rather
R6071 T7720 T7716 arg2Of pool,than
R6072 T7720 T7717 arg1Of pool,the
R6073 T7720 T7718 arg1Of pool,entire
R6074 T7720 T7719 arg1Of pool,cellular
R6075 T7720 T7721 arg1Of pool,of
R6076 T7723 T7721 arg2Of protein,of
R6077 T7723 T7722 arg1Of protein,this
R6078 T7727 T7726 arg1Of results,these
R6079 T7727 T7728 arg1Of results,support
R6080 T7728 T7724 arg1Of support,Together
R6081 T7728 T7725 arg1Of support,","
R6082 T7730 T7728 arg2Of idea,support
R6083 T7730 T7729 arg1Of idea,the
R6084 T7734 T7732 arg1Of proteins,the
R6085 T7734 T7733 arg1Of proteins,cIAP
R6086 T7734 T7735 arg1Of proteins,influence
R6087 T7735 T7730 arg2Of influence,idea
R6088 T7735 T7731 arg1Of influence,that
R6089 T7737 T7736 arg1Of recruitment,the
R6090 T7737 T7738 arg1Of recruitment,and
R6091 T7738 T7735 arg2Of and,influence
R6092 T7738 T7742 arg1Of and,of
R6093 T7741 T7738 arg2Of state,and
R6094 T7741 T7739 arg1Of state,post-translational
R6095 T7741 T7740 arg1Of state,modification
R6096 T7744 T7742 arg2Of HOIP,of
R6097 T7744 T7743 arg1Of HOIP,CD40-associated
R6098 T7746 T7745 arg1Of test,To
R6099 T7748 T7746 arg2Of possibility,test
R6100 T7748 T7747 arg1Of possibility,the
R6101 T7750 T7751 arg1Of HOIP,is
R6102 T7750 T7752 arg1Of HOIP,responsible
R6103 T7751 T7748 arg2Of is,possibility
R6104 T7751 T7749 arg1Of is,that
R6105 T7752 T7751 arg2Of responsible,is
R6106 T7752 T7753 arg1Of responsible,for
R6107 T7755 T7753 arg2Of recruitment,for
R6108 T7755 T7754 arg1Of recruitment,the
R6109 T7755 T7756 arg1Of recruitment,of
R6110 T7755 T7761 arg1Of recruitment,to
R6111 T7760 T7756 arg2Of proteins,of
R6112 T7760 T7757 arg1Of proteins,other
R6113 T7760 T7758 arg1Of proteins,critical
R6114 T7760 T7759 arg1Of proteins,signaling
R6115 T7762 T7761 arg2Of CD40,to
R6116 T7764 T7746 arg1Of we,test
R6117 T7764 T7765 arg1Of we,immunoprecipitated
R6118 T7765 T7745 modOf immunoprecipitated,To
R6119 T7765 T7763 arg1Of immunoprecipitated,","
R6120 T7768 T7765 arg2Of complexes,immunoprecipitated
R6121 T7768 T7766 arg1Of complexes,CD40
R6122 T7768 T7767 arg1Of complexes,signaling
R6123 T7768 T7769 arg1Of complexes,from
R6124 T7770 T7771 arg1Of HOIP-deficient,and
R6125 T7772 T7771 arg2Of HOIP-reconstituted,and
R6126 T7773 T7769 arg2Of cells,from
R6127 T7773 T7770 arg1Of cells,HOIP-deficient
R6128 T7773 T7772 arg1Of cells,HOIP-reconstituted
R6129 T7773 T7774 arg1Of cells,(
R6130 T7776 T7774 arg2Of 5B,(
R6131 T7776 T7775 arg1Of 5B,Fig.
R6132 T7777 T7774 arg3Of ),(
R6133 T7779 T7778 arg2Of described,As
R6134 T7779 T7780 arg1Of described,previously
R6135 T7784 T7782 arg1Of complexes,CD40
R6136 T7784 T7783 arg1Of complexes,signaling
R6137 T7784 T7785 arg2Of complexes,immunoprecipitated
R6138 T7784 T7789 arg1Of complexes,contained
R6139 T7785 T7786 arg1Of immunoprecipitated,from
R6140 T7788 T7786 arg2Of cells,from
R6141 T7788 T7787 arg1Of cells,A20.2J
R6142 T7789 T7778 arg1Of contained,As
R6143 T7789 T7781 arg1Of contained,","
R6144 T7790 T7793 arg1Of HOIP,as
R6145 T7793 T7789 arg2Of as,contained
R6146 T7793 T7791 arg1Of as,as
R6147 T7793 T7792 arg1Of as,well
R6148 T7794 T7795 arg1Of TRAF2,","
R6149 T7795 T7797 arg1Of ",",","
R6150 T7796 T7795 arg2Of TRAF3,","
R6151 T7797 T7800 arg1Of ",",and
R6152 T7798 T7797 arg2Of IKKα/β,","
R6153 T7800 T7793 arg2Of and,as
R6154 T7800 T7799 arg1Of and,","
R6155 T7801 T7800 arg2Of IKKγ,and
R6156 T7801 T7802 arg1Of IKKγ,[
R6157 T7803 T7802 arg2Of 6,[
R6158 T7804 T7802 arg3Of ],[
R6159 T7806 T7805 arg1Of amounts,The
R6160 T7861 T7859 arg2Of 5B,(
R6161 T7861 T7860 arg1Of 5B,Fig.
R6162 T7861 T7862 arg1Of 5B,","
R6163 T7806 T7807 arg1Of amounts,of
R6164 T7806 T7814 arg1Of amounts,in
R6165 T7863 T7862 arg2Of C,","
R6166 T7864 T7859 arg3Of ),(
R6167 T7806 T7820 arg1Of amounts,were
R6168 T7866 T7865 arg1Of amounts,The
R6169 T7866 T7867 arg1Of amounts,of
R6170 T7866 T7871 arg1Of amounts,in
R6171 T7866 T7877 arg1Of amounts,were
R6172 T7866 T7878 arg1Of amounts,similar
R6173 T7866 T7881 arg2Of amounts,detected
R6174 T7868 T7869 arg1Of IKKα/β,and
R6175 T7806 T7821 arg1Of amounts,similar
R6176 T7869 T7867 arg2Of and,of
R6177 T7808 T7809 arg1Of TRAF2,","
R6178 T7870 T7869 arg2Of IKKγ,and
R6179 T7873 T7871 arg2Of immunoprecipitates,in
R6180 T7873 T7872 arg1Of immunoprecipitates,CD40
R6181 T7873 T7874 arg1Of immunoprecipitates,from
R6182 T7809 T7812 arg1Of ",",and
R6183 T7876 T7874 arg2Of cells,from
R6184 T7876 T7875 arg1Of cells,HOIP-reconstituted
R6185 T7810 T7809 arg2Of TRAF3,","
R6186 T7878 T7879 arg1Of similar,to
R6187 T7880 T7879 arg2Of those,to
R6188 T7812 T7807 arg2Of and,of
R6189 T7881 T7877 arg2Of detected,were
R6190 T7881 T7878 modOf detected,similar
R6191 T7881 T7882 arg1Of detected,in
R6192 T7881 T7888 arg1Of detected,","
R6193 T7883 T7882 arg2Of samples,in
R6194 T7883 T7884 arg2Of samples,prepared
R6195 T7884 T7885 arg1Of prepared,from
R6196 T7812 T7811 arg1Of and,","
R6197 T7813 T7812 arg2Of cIAP1,and
R6198 T7887 T7885 arg2Of cells,from
R6199 T7887 T7886 arg1Of cells,parental
R6200 T7889 T7881 arg3Of demonstrating,detected
R6201 T7816 T7814 arg2Of immunoprecipitates,in
R6202 T7892 T7891 arg1Of defects,the
R6203 T7892 T7893 arg1Of defects,in
R6204 T7892 T7898 arg1Of defects,were
R6205 T7892 T7899 arg1Of defects,due
R6206 T7895 T7893 arg2Of recruitment,in
R6207 T7895 T7894 arg1Of recruitment,IKK
R6208 T7895 T7897 arg2Of recruitment,observed
R6209 T7896 T7897 arg1Of we,observed
R6210 T7816 T7815 arg1Of immunoprecipitates,CD40
R6211 T7898 T7889 arg2Of were,demonstrating
R6212 T7898 T7890 arg1Of were,that
R6213 T7816 T7817 arg1Of immunoprecipitates,from
R6214 T7899 T7898 arg2Of due,were
R6215 T7819 T7817 arg2Of cells,from
R6216 T7899 T7901 arg1Of due,to
R6217 T7901 T7900 arg1Of to,specifically
R6218 T7903 T7901 arg2Of absence,to
R6219 T7903 T7902 arg1Of absence,the
R6220 T7903 T7904 arg1Of absence,of
R6221 T7819 T7818 arg1Of cells,HOIP-deficient
R6222 T7820 T7828 arg1Of were,","
R6223 T7906 T7904 arg2Of expression,of
R6224 T7906 T7905 arg1Of expression,HOIP
R6225 T7908 T7907 arg1Of data,These
R6226 T7908 T7909 arg1Of data,indicate
R6227 T7820 T7829 modOf were,indicating
R6228 T7821 T7820 arg2Of similar,were
R6229 T7911 T7912 arg1Of HOIP,is
R6230 T7911 T7913 arg2Of HOIP,required
R6231 T7821 T7822 arg1Of similar,to
R6232 T7913 T7909 arg2Of required,indicate
R6233 T7913 T7910 arg1Of required,that
R6234 T7913 T7912 arg2Of required,is
R6235 T7913 T7914 arg1Of required,for
R6236 T7823 T7822 arg2Of those,to
R6237 T7915 T7914 arg2Of recruitment,for
R6238 T7915 T7916 arg1Of recruitment,of
R6239 T7915 T7920 arg1Of recruitment,to
R6240 T7823 T7824 arg1Of those,from
R6241 T7919 T7916 arg2Of complex,of
R6242 T7919 T7917 arg1Of complex,the
R6243 T7919 T7918 arg1Of complex,NF-κB-activating
R6244 T7827 T7824 arg2Of cells,from
R6245 T7924 T7920 arg2Of complex,to
R6246 T7924 T7921 arg1Of complex,the
R6247 T7924 T7922 arg1Of complex,CD40
R6248 T7924 T7923 arg1Of complex,signaling
R6249 T7926 T7925 arg2Of shown,As
R6250 T7827 T7825 arg1Of cells,parental
R6251 T7827 T7826 arg1Of cells,A20.2J
R6252 T7831 T7832 arg1Of HOIP,is
R6253 T7926 T7927 arg1Of shown,here
R6254 T7926 T7929 arg1Of shown,in
R6255 T7926 T7933 arg1Of shown,[
R6256 T7927 T7928 arg1Of here,and
R6257 T7929 T7928 arg2Of in,and
R6258 T7831 T7834 arg2Of HOIP,required
R6259 T7932 T7929 arg2Of study,in
R6260 T7932 T7930 arg1Of study,our
R6261 T7932 T7931 arg1Of study,previous
R6262 T7834 T7829 arg2Of required,indicating
R6263 T7934 T7933 arg2Of 6,[
R6264 T7935 T7933 arg3Of ],[
R6265 T7937 T7938 arg1Of IKKγ,present
R6266 T7937 T7942 arg1Of IKKγ,appears
R6267 T7937 T7944 arg1Of IKKγ,have
R6268 T7938 T7939 arg1Of present,in
R6269 T7941 T7939 arg2Of immunoprecipitates,in
R6270 T7941 T7940 arg1Of immunoprecipitates,CD40
R6271 T7834 T7830 arg1Of required,that
R6272 T7942 T7925 arg1Of appears,As
R6273 T7942 T7936 arg1Of appears,","
R6274 T7834 T7832 arg2Of required,is
R6275 T7944 T7942 arg2Of have,appears
R6276 T7944 T7943 arg1Of have,to
R6277 T7944 T7949 arg1Of have,than
R6278 T7834 T7833 arg1Of required,not
R6279 T7834 T7835 arg1Of required,for
R6280 T7837 T7835 arg2Of association,for
R6281 T7948 T7944 arg2Of weight,have
R6282 T7948 T7945 arg1Of weight,a
R6283 T7948 T7946 arg1Of weight,higher
R6284 T7948 T7947 arg1Of weight,molecular
R6285 T7950 T7949 arg2Of that,than
R6286 T7950 T7951 arg2Of that,found
R6287 T7951 T7952 arg1Of found,in
R6288 T7837 T7836 arg1Of association,the
R6289 T7954 T7952 arg2Of lysates,in
R6290 T7954 T7953 arg1Of lysates,cell
R6291 T7837 T7838 arg1Of association,of
R6292 T7956 T7955 arg1Of confirm,To
R6293 T7837 T7841 arg1Of association,with
R6294 T7840 T7838 arg2Of proteins,of
R6295 T7840 T7839 arg1Of proteins,these
R6296 T7842 T7841 arg2Of CD40,with
R6297 T7844 T7843 arg2Of contrast,In
R6298 T7846 T7847 arg1Of IKKα/β,and
R6299 T7962 T7958 arg1Of species,this
R6300 T7962 T7959 arg1Of species,higher
R6301 T7962 T7960 arg1Of species,molecular
R6302 T7962 T7961 arg1Of species,weight
R6303 T7962 T7963 arg1Of species,was
R6304 T7963 T7956 arg2Of was,confirm
R6305 T7963 T7957 arg1Of was,that
R6306 T7963 T7964 arg1Of was,indeed
R6307 T7965 T7963 arg2Of IKKγ,was
R6308 T7847 T7849 arg1Of and,were
R6309 T7967 T7956 arg1Of we,confirm
R6310 T7967 T7968 arg1Of we,generated
R6311 T7968 T7955 modOf generated,To
R6312 T7968 T7966 arg1Of generated,","
R6313 T7847 T7851 arg1Of and,detectable
R6314 T7971 T7968 arg2Of lines,generated
R6315 T7971 T7969 arg1Of lines,A20.2J
R6316 T7971 T7970 arg1Of lines,cell
R6317 T7971 T7972 arg1Of lines,containing
R6318 T7848 T7847 arg2Of IKKγ,and
R6319 T7975 T7974 arg1Of integrated,stably
R6320 T7849 T7843 arg1Of were,In
R6321 T7977 T7972 arg2Of vector,containing
R6322 T7977 T7973 arg1Of vector,a
R6323 T7977 T7975 arg1Of vector,integrated
R6324 T7977 T7976 arg1Of vector,retroviral
R6325 T7977 T7978 arg1Of vector,that
R6326 T7977 T7979 arg1Of vector,encoded
R6327 T7849 T7845 arg1Of were,","
R6328 T7849 T7850 arg1Of were,not
R6329 T7849 T7852 arg1Of were,in
R6330 T7982 T7979 arg2Of version,encoded
R6331 T7982 T7980 arg1Of version,an
R6332 T7982 T7981 arg1Of version,epitope-tagged
R6333 T7982 T7983 arg1Of version,of
R6334 T7851 T7849 arg2Of detectable,were
R6335 T7985 T7983 arg2Of IKKγ,of
R6336 T7985 T7984 arg1Of IKKγ,mouse
R6337 T7854 T7852 arg2Of immunoprecipitates,in
R6338 T7987 T7986 arg1Of lysates,Cell
R6339 T7987 T7988 arg1Of lysates,and
R6340 T7988 T7995 arg1Of and,were
R6341 T7988 T7996 arg2Of and,analyzed
R6342 T7990 T7988 arg2Of immunoprecipitates,and
R6343 T7990 T7989 arg1Of immunoprecipitates,CD40
R6344 T7990 T7991 arg2Of immunoprecipitates,prepared
R6345 T7991 T7992 arg1Of prepared,from
R6346 T7854 T7853 arg1Of immunoprecipitates,CD40
R6347 T7854 T7855 arg2Of immunoprecipitates,recovered
R6348 T7994 T7992 arg2Of cells,from
R6349 T7994 T7993 arg1Of cells,these
R6350 T7855 T7856 arg1Of recovered,from
R6351 T7996 T7995 arg2Of analyzed,were
R6352 T7996 T7997 arg1Of analyzed,by
R6353 T7858 T7856 arg2Of cells,from
R6354 T7999 T7997 arg2Of blotting,by
R6355 T7999 T7998 arg1Of blotting,Western
R6356 T7999 T8000 arg1Of blotting,for
R6357 T8003 T8000 arg2Of tag,for
R6358 T8003 T8001 arg1Of tag,the
R6359 T8003 T8002 arg1Of tag,epitope
R6360 T8003 T8004 arg1Of tag,(
R6361 T7858 T7857 arg1Of cells,HOIP-deficient
R6362 T7858 T7859 arg1Of cells,(
R6363 T8006 T8004 arg2Of 5C,(
R6364 T8006 T8005 arg1Of 5C,Fig.
R6365 T8054 T8053 arg2Of CD40,to
R6366 T8007 T8004 arg3Of ),(
R6367 T8009 T8008 arg1Of analysis,This
R6368 T8009 T8010 arg1Of analysis,produced
R6369 T8012 T8010 arg2Of pattern,produced
R6370 T8012 T8011 arg1Of pattern,a
R6371 T8012 T8013 arg1Of pattern,of
R6372 T8012 T8015 arg1Of pattern,that
R6373 T8012 T8016 arg1Of pattern,was
R6374 T8014 T8013 arg2Of bands,of
R6375 T8016 T8017 arg1Of was,essentially
R6376 T8016 T8023 modOf was,using
R6377 T8055 T8048 arg2Of requires,confirmed
R6378 T8019 T8016 arg2Of same,was
R6379 T8019 T8018 arg1Of same,the
R6380 T8019 T8020 arg1Of same,as
R6381 T8019 T8022 arg2Of same,obtained
R6382 T8021 T8020 arg2Of that,as
R6383 T8055 T8049 arg1Of requires,that
R6384 T8056 T8055 arg2Of HOIP,requires
R6385 T8025 T8023 arg2Of antibody,using
R6386 T8025 T8024 arg1Of antibody,an
R6387 T8025 T8026 arg1Of antibody,specific
R6388 T8026 T8027 arg1Of specific,for
R6389 T8058 T8057 arg1Of determine,To
R6390 T8029 T8027 arg2Of IKKγ,for
R6391 T8029 T8028 arg1Of IKKγ,native
R6392 T8029 T8030 arg1Of IKKγ,(
R6393 T8059 T8058 arg2Of what,determine
R6394 T8033 T8034 arg1Of B,and
R6395 T8034 T8030 arg2Of and,(
R6396 T8034 T8031 arg1Of and,compare
R6397 T8034 T8032 arg1Of and,panels
R6398 T8034 T8036 arg1Of and,in
R6399 T8035 T8034 arg2Of C,and
R6400 T8037 T8036 arg2Of Fig.,in
R6401 T8037 T8038 arg1Of Fig.,5
R6402 T8059 T8060 arg1Of what,was
R6403 T8039 T8030 arg3Of ),(
R6404 T8059 T8061 arg1Of what,responsible
R6405 T8061 T8060 arg2Of responsible,was
R6406 T8042 T8043 arg1Of analysis,of
R6407 T8042 T8048 arg1Of analysis,confirmed
R6408 T8044 T8043 arg2Of cells,of
R6409 T8044 T8045 arg1Of cells,expressing
R6410 T8061 T8062 arg1Of responsible,for
R6411 T8047 T8045 arg2Of tagged-IKKγ,expressing
R6412 T8047 T8046 arg1Of tagged-IKKγ,epitope
R6413 T8066 T8062 arg2Of weight,for
R6414 T8048 T8040 arg1Of confirmed,Moreover
R6415 T8048 T8041 arg1Of confirmed,","
R6416 T8050 T8051 arg1Of recruitment,of
R6417 T8050 T8053 arg1Of recruitment,to
R6418 T8050 T8055 arg1Of recruitment,requires
R6419 T8052 T8051 arg2Of IKKγ,of
R6420 T8066 T8063 arg1Of weight,the
R6421 T8066 T8064 arg2Of weight,increased
R6422 T8066 T8065 arg1Of weight,molecular
R6423 T8066 T8067 arg1Of weight,of
R6424 T8066 T8069 arg1Of weight,in
R6425 T8068 T8067 arg2Of IKKγ,of
R6426 T8071 T8069 arg2Of immunoprecipitates,in
R6427 T8152 T8149 arg3Of phosphorylation,in
R6428 T8071 T8070 arg1Of immunoprecipitates,CD40
R6429 T8156 T8155 arg1Of data,these
R6430 T8156 T8157 arg1Of data,demonstrate
R6431 T8156 T8171 arg1Of data,suggest
R6432 T8157 T8170 arg1Of demonstrate,and
R6433 T8075 T8073 arg1Of samples,the
R6434 T8159 T8160 arg1Of HOIP,is
R6435 T8159 T8161 arg2Of HOIP,required
R6436 T8161 T8157 arg2Of required,demonstrate
R6437 T8161 T8158 arg1Of required,that
R6438 T8161 T8160 arg2Of required,is
R6439 T8161 T8162 arg1Of required,for
R6440 T8164 T8162 arg2Of association,for
R6441 T8164 T8163 arg1Of association,the
R6442 T8164 T8165 arg1Of association,of
R6443 T8164 T8167 arg1Of association,with
R6444 T8166 T8165 arg2Of IKKγ,of
R6445 T8075 T8074 arg1Of samples,protein
R6446 T8075 T8076 arg1Of samples,were
R6447 T8168 T8167 arg2Of CD40,with
R6448 T8075 T8077 arg2Of samples,incubated
R6449 T8077 T8057 modOf incubated,To
R6450 T8170 T8153 arg1Of and,Overall
R6451 T8170 T8154 arg1Of and,","
R6452 T8170 T8169 arg1Of and,","
R6453 T8171 T8170 arg2Of suggest,and
R6454 T8174 T8173 arg1Of event,this
R6455 T8174 T8175 arg1Of event,is
R6456 T8174 T8176 arg2Of event,coupled
R6457 T8077 T8072 arg1Of incubated,","
R6458 T8176 T8171 arg2Of coupled,suggest
R6459 T8176 T8172 arg1Of coupled,that
R6460 T8176 T8175 arg2Of coupled,is
R6461 T8176 T8177 arg1Of coupled,to
R6462 T8077 T8076 arg2Of incubated,were
R6463 T8077 T8078 arg1Of incubated,with
R6464 T8179 T8177 arg2Of modifications,to
R6465 T8179 T8178 arg1Of modifications,post-translational
R6466 T8179 T8180 arg1Of modifications,of
R6467 T8080 T8078 arg2Of phosphatase,with
R6468 T8181 T8180 arg2Of IKKγ,of
R6469 T8080 T8079 arg1Of phosphatase,lambda
R6470 T8080 T8081 arg1Of phosphatase,","
R6471 T8080 T8082 arg1Of phosphatase,which
R6472 T8080 T8083 arg1Of phosphatase,removes
R6473 T8084 T8083 arg2Of phosphates,removes
R6474 T8084 T8085 arg2Of phosphates,attached
R6475 T8085 T8086 arg1Of attached,to
R6477 T8087 T8088 arg1Of tyrosine,","
R6481 T8088 T8091 arg1Of ",",or
R6483 T8089 T8088 arg2Of threonine,","
R6484 T8091 T8086 arg2Of or,to
R6485 T8091 T8090 arg1Of or,","
R6486 T8091 T8094 arg1Of or,in
R6487 T8093 T8091 arg2Of residues,or
R6488 T8093 T8092 arg1Of residues,serine
R6489 T8095 T8094 arg2Of proteins,in
R6490 T8097 T8096 arg1Of treatment,This
R6491 T8097 T8098 arg1Of treatment,reduced
R6492 T8099 T8098 arg2Of much,reduced
R6493 T8099 T8100 arg1Of much,of
R6494 T8102 T8100 arg2Of IKKγ,of
R6495 T8102 T8101 arg1Of IKKγ,CD40-associated
R6496 T8102 T8103 arg1Of IKKγ,to
R6497 T8107 T8103 arg2Of weight,to
R6498 T8107 T8104 arg1Of weight,an
R6499 T8107 T8105 arg1Of weight,apparent
R6500 T8107 T8106 arg1Of weight,molecular
R6501 T8107 T8108 arg1Of weight,similar
R6502 T8108 T8109 arg1Of similar,to
R6503 T8108 T8114 arg1Of similar,in
R6504 T8110 T8109 arg2Of that,to
R6505 T8110 T8111 arg1Of that,of
R6506 T8113 T8111 arg2Of protein,of
R6507 T8113 T8112 arg1Of protein,the
R6508 T8116 T8114 arg2Of lysates,in
R6509 T8116 T8115 arg1Of lysates,cell
R6510 T8116 T8117 arg1Of lysates,(
R6511 T8119 T8117 arg2Of 5C,(
R6512 T8119 T8118 arg1Of 5C,Fig.
R6513 T8120 T8117 arg3Of ),(
R6514 T8125 T8123 arg1Of one,at
R6515 T8125 T8124 arg1Of one,least
R6516 T8126 T8125 arg1Of band,one
R6517 T8126 T8127 arg1Of band,of
R6518 T8126 T8132 arg1Of band,remained
R6519 T8126 T8133 arg1Of band,after
R6520 T8129 T8128 arg1Of higher,significantly
R6521 T8131 T8127 arg2Of weight,of
R6522 T8131 T8129 arg1Of weight,higher
R6523 T8131 T8130 arg1Of weight,molecular
R6524 T8132 T8121 arg1Of remained,However
R6525 T8132 T8122 arg1Of remained,","
R6526 T8132 T8136 arg1Of remained,","
R6527 T8132 T8137 modOf remained,suggesting
R6528 T8133 T8132 arg2Of after,remained
R6529 T8135 T8133 arg2Of treatment,after
R6530 T8135 T8134 arg1Of treatment,phosphatase
R6531 T8140 T8139 arg1Of IKKγ,CD40-associated
R6532 T8140 T8141 arg1Of IKKγ,is
R6533 T8140 T8142 arg1Of IKKγ,subject
R6534 T8141 T8137 arg2Of is,suggesting
R6535 T8141 T8138 arg1Of is,that
R6536 T8142 T8141 arg2Of subject,is
R6537 T8142 T8143 arg1Of subject,to
R6538 T8146 T8144 arg1Of one,at
R6539 T8146 T8145 arg1Of one,least
R6540 T8148 T8143 arg2Of modification,to
R6541 T8148 T8146 arg1Of modification,one
R6542 T8148 T8147 arg1Of modification,other
R6543 T8148 T8149 arg1Of modification,in
R6544 T8149 T8151 arg1Of in,to
R6545 T8150 T8149 arg2Of addition,in
R11923 T15029 T15027 arg1Of targeting,HOIP
R11924 T15029 T15028 arg1Of targeting,gene
R11925 T15031 T15030 arg2Of A,(
R11926 T15032 T15030 arg3Of ),(
R11927 T15033 T15030 arg1Of Regions,(
R11928 T15033 T15034 arg1Of Regions,of
R11929 T15033 T15041 arg2Of Regions,used
R11930 T15033 T15051 arg1Of Regions,are
R11931 T15033 T15052 arg2Of Regions,shown
R11934 T15037 T15036 arg1Of sequence,gene
R11935 T15037 T15038 arg1Of sequence,(
R11936 T15039 T15038 arg2Of bottom,(
R11937 T15040 T15038 arg3Of ),(
R11938 T15041 T15042 arg1Of used,in
R11939 T15047 T15042 arg2Of vector,in
R11940 T15047 T15043 arg1Of vector,the
R11941 T15047 T15044 arg1Of vector,HOIP
R11942 T15047 T15045 arg1Of vector,gene
R11943 T15047 T15046 arg1Of vector,targeting
R11944 T15047 T15048 arg1Of vector,(
R11945 T15049 T15048 arg2Of top,(
R11946 T15050 T15048 arg3Of ),(
R11947 T15052 T15051 arg2Of shown,are
R11948 T15053 T15054 arg1Of Arrows,(
R11949 T15053 T15059 arg1Of Arrows,indicate
R11950 T15055 T15056 arg1Of F,and
R11951 T15056 T15054 arg2Of and,(
R11952 T15057 T15056 arg2Of R,and
R11953 T15058 T15054 arg3Of ),(
R11954 T15061 T15059 arg2Of positions,indicate
R11955 T15061 T15060 arg1Of positions,approximate
R11956 T15061 T15062 arg1Of positions,of
R11957 T15063 T15062 arg2Of sequences,of
R11958 T15063 T15064 arg1Of sequences,homologous
R11959 T15064 T15065 arg1Of homologous,to
R11960 T15066 T15065 arg2Of oligonucleotides,to
R11961 T15066 T15067 arg2Of oligonucleotides,used
R11962 T15067 T15068 arg1Of used,for
R11963 T15070 T15068 arg2Of detection,for
R11964 T15070 T15069 arg1Of detection,PCR-mediated
R11965 T15070 T15071 arg1Of detection,of
R11966 T15073 T15071 arg2Of recombination,of
R11967 T15073 T15072 arg1Of recombination,homologous
R11968 T15075 T15074 arg1Of recombination,Homologous
R11969 T15075 T15076 arg1Of recombination,of
R11970 T15075 T15081 arg1Of recombination,resulted
R11971 T15078 T15076 arg2Of vector,of
R11972 T15078 T15077 arg1Of vector,the
R11973 T15078 T15079 arg1Of vector,with
R11974 T15080 T15079 arg2Of Rnf31,with
R11975 T15081 T15082 arg1Of resulted,in
R11976 T15086 T15083 arg1Of deletion,a
R11977 T15086 T15084 arg1Of deletion,small
R11978 T15086 T15085 arg1Of deletion,chromosomal
R11979 T15086 T15087 arg1Of deletion,and
R11980 T15087 T15082 arg2Of and,in
R11981 T15090 T15087 arg2Of insertion,and
R11982 T15090 T15088 arg1Of insertion,the
R11983 T15090 T15089 arg1Of insertion,in-frame
R11984 T15090 T15091 arg1Of insertion,of
R11985 T15090 T15097 arg1Of insertion,into
R11986 T15093 T15091 arg2Of phosphotransferase,of
R11987 T15093 T15092 arg1Of phosphotransferase,neomycin
R11988 T15093 T15094 arg1Of phosphotransferase,(
R11989 T15095 T15094 arg2Of NeoR,(
R11990 T15096 T15094 arg3Of ),(
R11991 T15100 T15097 arg2Of HOIP,into
R11992 T15100 T15098 arg1Of HOIP,the
R11993 T15100 T15099 arg1Of HOIP,amino-terminal
R11994 T15100 T15101 arg1Of HOIP,coding
R11995 T15102 T15101 arg2Of sequence,coding
R11996 T15105 T15104 arg1Of toxin,diphtheria
R11997 T15105 T15106 arg1Of toxin,(
R11998 T15107 T15106 arg2Of DT,(
R11999 T15108 T15106 arg3Of ),(
R12000 T15109 T15103 arg1Of cassette,A
R12001 T15109 T15105 arg1Of cassette,toxin
R12002 T15109 T15110 arg1Of cassette,in
R12003 T15109 T15113 arg1Of cassette,facilitates
R12004 T15112 T15110 arg2Of vector,in
R12005 T15112 T15111 arg1Of vector,the
R12006 T15116 T15113 arg2Of selection,facilitates
R12007 T15116 T15114 arg1Of selection,the
R12008 T15116 T15115 arg1Of selection,negative
R12009 T15116 T15117 arg1Of selection,of
R12010 T15118 T15117 arg2Of cells,of
R12011 T15118 T15119 arg1Of cells,in
R12012 T15118 T15120 arg1Of cells,which
R12013 T15118 T15121 arg1Of cells,random
R12014 T15123 T15121 arg2Of insertion,random
R12015 T15123 T15122 arg1Of insertion,chromosomal
R12016 T15123 T15124 arg1Of insertion,of
R12017 T15123 T15127 arg1Of insertion,takes
R12018 T15126 T15124 arg2Of vector,of
R12019 T15126 T15125 arg1Of vector,the
R12020 T15127 T15121 arg3Of takes,random
R12021 T15128 T15127 arg2Of place,takes
R12022 T15130 T15129 arg1Of sequences,LoxP
R12023 T15130 T15131 arg1Of sequences,allow
R12024 T15134 T15131 arg2Of deletion,allow
R12025 T15134 T15132 arg1Of deletion,the
R12026 T15134 T15133 arg1Of deletion,Cre-mediated
R12027 T15134 T15135 arg1Of deletion,of
R12028 T15139 T15135 arg2Of sequence,of
R12029 T15139 T15136 arg1Of sequence,the
R12030 T15139 T15137 arg1Of sequence,NeoR
R12031 T15139 T15138 arg1Of sequence,coding
R12032 T15142 T15140 arg1Of sequence,The
R12033 T15142 T15141 arg1Of sequence,SV40pA
R12034 T15142 T15143 arg1Of sequence,helps
R12035 T15142 T15145 arg1Of sequence,ensure
R12036 T15145 T15143 arg2Of ensure,helps
R12037 T15145 T15144 arg1Of ensure,to
R12038 T15146 T15147 arg1Of disruption,of
R12039 T15146 T15150 arg1Of disruption,after
R12040 T15146 T15160 arg1Of disruption,and
R12041 T15149 T15147 arg2Of expression,of
R12042 T15149 T15148 arg1Of expression,gene
R12043 T15151 T15150 arg2Of deletion,after
R12044 T15151 T15152 arg1Of deletion,of
R12045 T15155 T15154 arg1Of sequence.,NeoR
R12046 T15155 T15156 arg1Of sequence.,(
R12047 T15157 T15156 arg2Of B,(
R12048 T15158 T15156 arg3Of ),(
R12049 T15159 T15152 arg2Of Anti-HOIP,of
R12050 T15159 T15153 arg1Of Anti-HOIP,the
R12051 T15159 T15155 arg1Of Anti-HOIP,sequence.
R12052 T15160 T15145 arg2Of and,ensure
R12053 T15163 T15160 arg2Of blots,and
R12054 T15163 T15161 arg1Of blots,anti-FLAG
R12055 T15163 T15162 arg1Of blots,Western
R12056 T15163 T15164 arg1Of blots,of
R12057 T15166 T15165 arg1Of lysates,cell
R12058 T15166 T15167 arg1Of lysates,from
R12059 T15166 T15170 arg1Of lysates,and
R12060 T15169 T15167 arg2Of cells,from
R12061 T15169 T15168 arg1Of cells,A20.2J
R12062 T15170 T15164 arg2Of and,of
R12063 T15171 T15172 arg1Of HOIP-deficient,(
R12064 T15173 T15172 arg2Of HOIP-/-,(
R12065 T15174 T15172 arg3Of ),(
R12066 T15175 T15170 arg2Of clones,and
R12067 T15175 T15171 arg1Of clones,HOIP-deficient
R12068 T15178 T15176 arg1Of decrease,A
R12069 T15178 T15177 arg1Of decrease,partial
R12070 T15178 T15179 arg1Of decrease,in
R12071 T15178 T15183 arg1Of decrease,is
R12072 T15178 T15184 arg1Of decrease,evident
R12073 T15182 T15179 arg2Of expression,in
R12074 T15182 T15180 arg1Of expression,HOIP
R12075 T15182 T15181 arg1Of expression,protein
R12076 T15183 T15185 arg1Of is,in
R12077 T15183 T15187 arg1Of is,following
R12078 T15184 T15183 arg2Of evident,is
R12079 T15186 T15185 arg2Of cells,in
R12080 T15188 T15187 arg2Of disruption,following
R12081 T15188 T15189 arg1Of disruption,of
R12082 T15191 T15189 arg2Of copy,of
R12083 T15191 T15190 arg1Of copy,one
R12084 T15191 T15192 arg1Of copy,of
R12085 T15193 T15192 arg2Of Rnf31,of
R12086 T15193 T15194 arg1Of Rnf31,(
R12087 T15195 T15194 arg2Of HOIP−/+,(
R12088 T15196 T15194 arg3Of ),(
R12089 T15198 T15197 arg1Of expression,HOIP
R12090 T15198 T15199 arg1Of expression,in
R12091 T15198 T15217 arg1Of expression,is
R12092 T15198 T15219 arg2Of expression,shown
R12093 T15200 T15199 arg2Of clones,in
R12094 T15200 T15201 arg2Of clones,reconstituted
R12095 T15201 T15202 arg1Of reconstituted,with
R12096 T15206 T15203 arg1Of vector,an
R12097 T15206 T15204 arg1Of vector,empty
R12098 T15206 T15205 arg1Of vector,retroviral
R12099 T15206 T15207 arg1Of vector,(
R12100 T15206 T15210 arg1Of vector,or
R12101 T15208 T15207 arg2Of pMIP,(
R12102 T15209 T15207 arg3Of ),(
R12103 T15210 T15202 arg2Of or,with
R12104 T15213 T15210 arg2Of vector,or
R12105 T15213 T15211 arg1Of vector,a
R12106 T15213 T15212 arg1Of vector,retroviral
R12107 T15213 T15214 arg1Of vector,encoding
R12108 T15216 T15214 arg2Of HOIP,encoding
R12109 T15216 T15215 arg1Of HOIP,FLAG-tagged
R12110 T15219 T15217 arg2Of shown,is
R12111 T15219 T15218 arg1Of shown,also
R12112 T15222 T15220 arg1Of weight,Approximate
R12113 T15222 T15221 arg1Of weight,molecular
R12114 T15222 T15223 arg1Of weight,of
R12115 T15222 T15225 arg1Of weight,is
R12116 T15224 T15223 arg2Of HOIP,of
R12117 T15227 T15225 arg2Of kD,is
R12118 T15227 T15226 arg1Of kD,120
R12278 T15492 T15490 arg1Of upregulation,CD40-mediated
R12279 T15492 T15491 arg1Of upregulation,CD80
R12280 T15492 T15493 arg1Of upregulation,is
R12281 T15492 T15494 arg1Of upregulation,defective
R12282 T15493 T15495 arg1Of is,in
R12283 T15494 T15493 arg2Of defective,is
R12284 T15497 T15495 arg2Of cells,in
R12285 T15497 T15496 arg1Of cells,HOIP-deficient
R12286 T15498 T15499 arg1Of Cells,were
R12287 T15498 T15500 arg2Of Cells,incubated
R12288 T15498 T15520 arg2Of Cells,stained
R12289 T15500 T15501 arg1Of incubated,for
R12290 T15500 T15518 arg1Of incubated,and
R12291 T15503 T15501 arg2Of hrs,for
R12292 T15503 T15502 arg1Of hrs,72
R12293 T15503 T15504 arg1Of hrs,with
R12294 T15508 T15506 arg1Of antibody,isotype
R12295 T15508 T15507 arg1Of antibody,control
R12296 T15508 T15509 arg1Of antibody,(
R12297 T15508 T15512 arg1Of antibody,or
R12298 T15510 T15509 arg2Of left,(
R12299 T15511 T15509 arg3Of ),(
R12300 T15512 T15504 arg2Of or,with
R12301 T15512 T15505 arg1Of or,an
R12302 T15514 T15512 arg2Of antibody,or
R12303 T15514 T15513 arg1Of antibody,anti-CD40
R12304 T15514 T15515 arg1Of antibody,(
R12305 T15516 T15515 arg2Of right,(
R12306 T15517 T15515 arg3Of ),(
R12307 T15518 T15499 arg2Of and,were
R12308 T15520 T15518 arg2Of stained,and
R12309 T15520 T15519 arg1Of stained,then
R12310 T15520 T15521 arg1Of stained,for
R12311 T15522 T15521 arg2Of expression,for
R12312 T15522 T15523 arg1Of expression,of
R12313 T15524 T15523 arg2Of CD80,of
R12314 T15524 T15525 arg1Of CD80,(
R12315 T15527 T15526 arg2Of profiles,filled
R12316 T15527 T15528 arg1Of profiles,indicate
R12317 T15528 T15525 arg2Of indicate,(
R12318 T15528 T15530 arg1Of indicate,with
R12319 T15528 T15533 arg1Of indicate,;
R12320 T15529 T15528 arg2Of staining,indicate
R12321 T15532 T15530 arg2Of antibody,with
R12322 T15532 T15531 arg1Of antibody,anti-CD80
R12323 T15535 T15534 arg1Of profiles,open
R12324 T15535 T15536 arg1Of profiles,are
R12325 T15535 T15537 arg1Of profiles,staining
R12326 T15537 T15533 arg2Of staining,;
R12327 T15537 T15536 arg2Of staining,are
R12328 T15537 T15538 arg1Of staining,with
R12329 T15542 T15538 arg2Of antibody,with
R12330 T15542 T15539 arg1Of antibody,an
R12331 T15542 T15540 arg1Of antibody,isotype
R12332 T15542 T15541 arg1Of antibody,control
R12333 T15543 T15525 arg3Of ),(
R12334 T15544 T15545 arg1Of Transduction,of
R12335 T15544 T15557 arg1Of Transduction,reversed
R12336 T15547 T15548 arg1Of HOIP-deficient,(
R12337 T15549 T15548 arg2Of HOIP-/-,(
R12338 T15550 T15548 arg3Of ),(
R12339 T15551 T15545 arg2Of clones,of
R12340 T15551 T15546 arg1Of clones,both
R12341 T15551 T15547 arg1Of clones,HOIP-deficient
R12342 T15551 T15552 arg1Of clones,with
R12343 T15554 T15552 arg2Of retrovirus,with
R12344 T15554 T15553 arg1Of retrovirus,a
R12345 T15554 T15555 arg1Of retrovirus,encoding
R12346 T15556 T15555 arg2Of FLAG-HOIP,encoding
R12347 T15557 T15561 arg1Of reversed,","
R12348 T15557 T15562 arg1Of reversed,while
R12349 T15560 T15557 arg2Of upregulation,reversed
R12350 T15560 T15558 arg1Of upregulation,defective
R12351 T15560 T15559 arg1Of upregulation,CD80
R12352 T15563 T15564 arg1Of transduction,with
R12353 T15563 T15571 arg1Of transduction,did
R12354 T15567 T15564 arg2Of vector,with
R12355 T15567 T15565 arg1Of vector,an
R12356 T15567 T15566 arg1Of vector,empty
R12357 T15567 T15568 arg1Of vector,(
R12358 T15569 T15568 arg2Of pMIP,(
R12359 T15570 T15568 arg3Of ),(
R12360 T15571 T15562 arg2Of did,while
R12361 T15571 T15572 arg1Of did,not
R12362 T15574 T15573 arg1Of results,Similar
R12363 T15574 T15575 arg1Of results,were
R12364 T15574 T15576 arg2Of results,obtained
R12365 T15576 T15575 arg2Of obtained,were
R12366 T15576 T15577 arg1Of obtained,in
R12367 T15580 T15577 arg2Of experiment,in
R12368 T15580 T15578 arg1Of experiment,a
R12369 T15580 T15579 arg1Of experiment,second
R12576 T15890 T15889 arg1Of transcription,GLε
R12577 T15890 T15891 arg1Of transcription,is
R12578 T15890 T15892 arg1Of transcription,defective
R12579 T15892 T15891 arg2Of defective,is
R12580 T15892 T15893 arg1Of defective,in
R12581 T15896 T15893 arg2Of cells,in
R12582 T15896 T15894 arg1Of cells,CD40-stimulated
R12583 T15896 T15895 arg1Of cells,HOIP-deficient
R12584 T15900 T15897 arg1Of cells,A20.2J
R12585 T15900 T15898 arg1Of cells,and
R12586 T15900 T15899 arg1Of cells,HOIP-deficient
R12587 T15900 T15901 arg2Of cells,transduced
R12588 T15900 T15907 arg1Of cells,(
R12589 T15900 T15919 arg1Of cells,or
R12590 T15901 T15902 arg1Of transduced,with
R12591 T15906 T15902 arg2Of vector,with
R12592 T15906 T15903 arg1Of vector,an
R12593 T15906 T15904 arg1Of vector,empty
R12594 T15906 T15905 arg1Of vector,retroviral
R12595 T15910 T15908 arg1Of pMIP,A20.2J
R12596 T15910 T15909 arg1Of pMIP,+
R12597 T15910 T15911 arg1Of pMIP,and
R12598 T15912 T15911 arg2Of HOIP-/-,and
R12599 T15914 T15907 arg2Of pMIP,(
R12600 T15914 T15910 arg1Of pMIP,pMIP
R12601 T15914 T15912 arg1Of pMIP,HOIP-/-
R12602 T15914 T15913 arg1Of pMIP,+
R12603 T15914 T15915 arg1Of pMIP,","
R12604 T15914 T15916 arg1Of pMIP,respectively
R12605 T15917 T15907 arg3Of ),(
R12606 T15919 T15918 arg1Of or,","
R12607 T15919 T15932 arg1Of or,were
R12608 T15919 T15933 arg2Of or,cultured
R12609 T15921 T15919 arg2Of cells,or
R12610 T15921 T15920 arg1Of cells,HOIP-deficient
R12611 T15921 T15922 arg2Of cells,transduced
R12612 T15922 T15923 arg1Of transduced,with
R12613 T15926 T15923 arg2Of retrovirus,with
R12614 T15926 T15924 arg1Of retrovirus,a
R12615 T15926 T15925 arg1Of retrovirus,HOIP-encoding
R12616 T15926 T15927 arg1Of retrovirus,(
R12617 T15930 T15927 arg2Of HOIP,(
R12618 T15930 T15928 arg1Of HOIP,HOIP-/-
R12619 T15930 T15929 arg1Of HOIP,+
R12620 T15931 T15927 arg3Of ),(
R12621 T15933 T15932 arg2Of cultured,were
R12622 T15933 T15934 arg1Of cultured,overnight
R12623 T15933 T15935 arg1Of cultured,with
R12624 T15933 T15946 arg1Of cultured,","
R12625 T15933 T15947 arg1Of cultured,with
R12626 T15933 T15949 arg1Of cultured,without
R12627 T15937 T15936 arg1Of anti-CD40,agonistic
R12628 T15937 T15938 arg1Of anti-CD40,or
R12629 T15938 T15935 arg2Of or,with
R12630 T15942 T15938 arg2Of antibody,or
R12631 T15942 T15939 arg1Of antibody,an
R12632 T15942 T15940 arg1Of antibody,isotype
R12633 T15942 T15941 arg1Of antibody,control
R12634 T15942 T15943 arg1Of antibody,(
R12635 T15944 T15943 arg2Of iso,(
R12636 T15945 T15943 arg3Of ),(
R12637 T15947 T15948 arg1Of with,or
R12638 T15949 T15948 arg2Of without,or
R12639 T15950 T15947 arg2Of IL-4,with
R12640 T15950 T15949 arg2Of IL-4,without
R12641 T15951 T15952 arg1Of RNA,was
R12642 T15951 T15953 arg2Of RNA,isolated
R12643 T15951 T15962 arg2Of RNA,subjected
R12644 T15953 T15954 arg1Of isolated,from
R12645 T15953 T15960 arg1Of isolated,and
R12646 T15956 T15954 arg2Of cells,from
R12647 T15956 T15955 arg1Of cells,the
R12648 T15956 T15957 arg1Of cells,","
R12649 T15958 T15957 arg2Of reverse-transcribed,","
R12650 T15960 T15952 arg2Of and,was
R12651 T15960 T15959 arg1Of and,","
R12652 T15962 T15960 arg2Of subjected,and
R12653 T15962 T15961 arg1Of subjected,then
R12654 T15962 T15963 arg1Of subjected,to
R12655 T15962 T15966 modOf subjected,to
R12656 T15965 T15963 arg2Of PCR,to
R12657 T15965 T15964 arg1Of PCR,quantitative
R12658 T15967 T15966 arg1Of determine,to
R12659 T15968 T15967 arg2Of levels,determine
R12660 T15968 T15969 arg1Of levels,of
R12661 T15971 T15969 arg2Of transcripts,of
R12662 T15971 T15970 arg1Of transcripts,GLε
R12663 T15972 T15973 arg1Of Results,were
R12664 T15972 T15974 arg2Of Results,normalized
R12665 T15974 T15973 arg2Of normalized,were
R12666 T15974 T15975 arg1Of normalized,to
R12667 T15977 T15975 arg2Of levels,to
R12668 T15977 T15976 arg1Of levels,the
R12669 T15977 T15978 arg1Of levels,of
R12670 T15977 T15981 arg1Of levels,in
R12671 T15980 T15978 arg2Of transcripts,of
R12672 T15980 T15979 arg1Of transcripts,Hprt1
R12673 T15983 T15981 arg2Of sample,in
R12674 T15983 T15982 arg1Of sample,each
R12675 T15984 T15985 arg1Of Symbols,indicate
R12676 T15985 T15991 arg1Of indicate,(
R12677 T15987 T15985 arg2Of values,indicate
R12678 T15987 T15986 arg1Of values,the
R12679 T15987 T15988 arg1Of values,from
R12680 T15990 T15988 arg2Of cultures,from
R12681 T15990 T15989 arg1Of cultures,duplicate
R12682 T15993 T15992 arg1Of line,a
R12683 T15993 T15994 arg1Of line,indicates
R12684 T15994 T15991 arg2Of indicates,(
R12685 T15996 T15994 arg2Of mean,indicates
R12686 T15996 T15995 arg1Of mean,the
R12687 T15996 T15997 arg1Of mean,of
R12688 T16000 T15997 arg2Of values,of
R12689 T16000 T15998 arg1Of values,the
R12690 T16000 T15999 arg1Of values,two
R12691 T16001 T15991 arg3Of ),(
R12692 T16003 T16002 arg1Of results,Similar
R12693 T16003 T16004 arg1Of results,were
R12694 T16003 T16005 arg2Of results,obtained
R12695 T16005 T16004 arg2Of obtained,were
R12696 T16005 T16006 arg1Of obtained,in
R12697 T16005 T16011 arg1Of obtained,in
R12698 T16005 T16015 arg1Of obtained,with
R12699 T16006 T16010 arg1Of in,and
R12700 T16009 T16006 arg2Of experiment,in
R12701 T16009 T16007 arg1Of experiment,a
R12702 T16009 T16008 arg1Of experiment,second
R12703 T16011 T16010 arg2Of in,and
R12704 T16014 T16011 arg2Of experiment,in
R12705 T16014 T16012 arg1Of experiment,an
R12706 T16014 T16013 arg1Of experiment,additional
R12707 T16019 T16015 arg2Of clone,with
R12708 T16019 T16016 arg1Of clone,a
R12709 T16019 T16017 arg1Of clone,second
R12710 T16019 T16018 arg1Of clone,HOIP-deficient
R12939 T16375 T16374 arg1Of activation,CD40-induced
R12940 T16375 T16376 arg1Of activation,of
R12941 T16375 T16380 arg1Of activation,is
R12942 T16375 T16381 arg1Of activation,defective
R12943 T16377 T16378 arg1Of NF-κB,and
R12944 T16378 T16376 arg2Of and,of
R12945 T16379 T16378 arg2Of JNK,and
R12946 T16380 T16382 arg1Of is,in
R12947 T16381 T16380 arg2Of defective,is
R12948 T16384 T16382 arg2Of cells,in
R12949 T16384 T16383 arg1Of cells,HOIP-deficient
R12950 T16386 T16385 arg1Of signaling,CD40-mediated
R12951 T16386 T16387 arg1Of signaling,was
R12952 T16386 T16388 arg1Of signaling,defective
R12953 T16386 T16399 arg1Of signaling,intact
R12954 T16388 T16389 arg1Of defective,in
R12955 T16388 T16398 arg1Of defective,but
R12956 T16391 T16389 arg2Of cells,in
R12957 T16391 T16390 arg1Of cells,HOIP-deficient
R12958 T16391 T16392 arg1Of cells,(
R12959 T16393 T16392 arg2Of HOIP-/-,(
R12960 T16393 T16394 arg1Of HOIP-/-,+
R12961 T16393 T16395 arg1Of HOIP-/-,pMIP
R12962 T16396 T16392 arg3Of ),(
R12963 T16398 T16387 arg2Of but,was
R12964 T16398 T16397 arg1Of but,","
R12965 T16399 T16398 arg2Of intact,but
R12966 T16399 T16400 arg1Of intact,in
R12967 T16402 T16400 arg2Of cells,in
R12968 T16402 T16401 arg1Of cells,parental
R12969 T16402 T16403 arg2Of cells,transduced
R12970 T16403 T16404 arg1Of transduced,with
R12971 T16403 T16415 arg1Of transduced,in
R12972 T16404 T16414 arg1Of with,and
R12973 T16408 T16404 arg2Of vector,with
R12974 T16408 T16405 arg1Of vector,an
R12975 T16408 T16406 arg1Of vector,empty
R12976 T16408 T16407 arg1Of vector,retroviral
R12977 T16408 T16409 arg1Of vector,(
R12978 T16410 T16409 arg2Of A20.2J,(
R12979 T16410 T16411 arg1Of A20.2J,+
R12980 T16410 T16412 arg1Of A20.2J,pMIP
R12981 T16413 T16409 arg3Of ),(
R12982 T16415 T16414 arg2Of in,and
R12983 T16417 T16415 arg2Of cells,in
R12984 T16417 T16416 arg1Of cells,HOIP-deficient
R12985 T16417 T16418 arg2Of cells,transduced
R12986 T16418 T16419 arg1Of transduced,with
R12987 T16422 T16419 arg2Of vector,with
R12988 T16422 T16420 arg1Of vector,a
R12989 T16422 T16421 arg1Of vector,retroviral
R12990 T16422 T16423 arg1Of vector,encoding
R12991 T16424 T16423 arg2Of HOIP,encoding
R12992 T16424 T16425 arg1Of HOIP,(
R12993 T16426 T16425 arg2Of HOIP-/-,(
R12994 T16426 T16427 arg1Of HOIP-/-,+
R12995 T16426 T16428 arg1Of HOIP-/-,HOIP
R12996 T16429 T16425 arg3Of ),(
R12997 T16430 T16431 arg1Of Cells,were
R12998 T16430 T16432 arg2Of Cells,activated
R12999 T16432 T16431 arg2Of activated,were
R13000 T16432 T16433 arg1Of activated,with
R13001 T16432 T16440 arg2Of activated,indicated
R13002 T16435 T16433 arg2Of insect,with
R13003 T16435 T16434 arg1Of insect,CD154-expressing
R13004 T16436 T16437 arg1Of cells,for
R13005 T16436 T16440 arg1Of cells,indicated
R13006 T16439 T16437 arg2Of times,for
R13007 T16439 T16438 arg1Of times,the
R13008 T16444 T16441 arg2Of control,As
R13009 T16444 T16442 arg1Of control,a
R13010 T16444 T16443 arg1Of control,negative
R13011 T16446 T16447 arg1Of cells,were
R13012 T16446 T16448 arg2Of cells,stimulated
R13013 T16448 T16441 arg1Of stimulated,As
R13014 T16448 T16445 arg1Of stimulated,","
R13015 T16448 T16447 arg2Of stimulated,were
R13016 T16448 T16449 arg1Of stimulated,with
R13017 T16451 T16449 arg2Of cells,with
R13018 T16451 T16450 arg1Of cells,insect
R13019 T16451 T16452 arg1Of cells,lacking
R13020 T16453 T16452 arg2Of CD154,lacking
R13021 T16453 T16454 arg1Of CD154,(
R13022 T16458 T16454 arg2Of point,(
R13023 T16458 T16455 arg1Of point,5
R13024 T16458 T16456 arg1Of point,minute
R13025 T16458 T16457 arg1Of point,time
R13026 T16458 T16459 arg1Of point,only
R13027 T16460 T16454 arg3Of ),(
R13028 T16462 T16461 arg1Of blots,Western
R13029 T16462 T16463 arg1Of blots,of
R13030 T16462 T16466 arg1Of blots,were
R13031 T16462 T16467 arg2Of blots,probed
R13032 T16465 T16463 arg2Of lysates,of
R13033 T16465 T16464 arg1Of lysates,whole-cell
R13034 T16467 T16466 arg2Of probed,were
R13035 T16467 T16468 arg1Of probed,with
R13036 T16471 T16468 arg2Of antibodies,with
R13037 T16471 T16469 arg1Of antibodies,the
R13038 T16471 T16470 arg1Of antibodies,indicated
R13039 T16472 T16473 arg1Of Phospho-IκBα,(
R13040 T16472 T16476 arg1Of Phospho-IκBα,and
R13041 T16474 T16473 arg2Of pIκBα,(
R13042 T16475 T16473 arg3Of ),(
R13043 T16476 T16482 arg1Of and,were
R13044 T16476 T16483 arg2Of and,stripped
R13045 T16476 T16485 arg2Of and,reprobed
R13046 T16479 T16478 arg2Of pJNK,(
R13047 T16480 T16478 arg3Of ),(
R13048 T16481 T16476 arg2Of blots,and
R13049 T16481 T16477 arg1Of blots,phospho-JNK
R13050 T16481 T16478 arg1Of blots,(
R13051 T16483 T16484 arg1Of stripped,and
R13052 T16484 T16482 arg2Of and,were
R13053 T16484 T16486 arg1Of and,for
R13054 T16484 T16492 arg1Of and,","
R13055 T16485 T16484 arg2Of reprobed,and
R13056 T16488 T16487 arg1Of IκBα,total
R13057 T16488 T16489 arg1Of IκBα,and
R13058 T16489 T16486 arg2Of and,for
R13059 T16491 T16489 arg2Of JNK,and
R13060 T16491 T16490 arg1Of JNK,total
R13061 T16496 T16495 arg1Of antibodies,anti-JNK
R13062 T16496 T16497 arg1Of antibodies,recognize
R13063 T16497 T16492 arg2Of recognize,","
R13064 T16497 T16493 arg1Of recognize,respectively
R13065 T16497 T16494 arg1Of recognize,(
R13066 T16497 T16502 arg1Of recognize,)
R13067 T16498 T16499 arg1Of p46,and
R13068 T16500 T16499 arg2Of p54,and
R13069 T16501 T16497 arg2Of isoforms,recognize
R13070 T16501 T16498 arg1Of isoforms,p46
R13071 T16501 T16500 arg1Of isoforms,p54
R13072 T16504 T16503 arg1Of blots,IκBα
R13073 T16504 T16505 arg1Of blots,were
R13074 T16504 T16507 arg2Of blots,reprobed
R13075 T16504 T16511 arg1Of blots,demonstrate
R13076 T16507 T16505 arg2Of reprobed,were
R13077 T16507 T16506 arg1Of reprobed,also
R13078 T16507 T16508 arg1Of reprobed,for
R13079 T16509 T16508 arg2Of actin,for
R13080 T16511 T16507 arg3Of demonstrate,reprobed
R13081 T16511 T16510 arg1Of demonstrate,to
R13082 T16514 T16511 arg2Of loading,demonstrate
R13083 T16514 T16512 arg1Of loading,equal
R13084 T16514 T16513 arg1Of loading,lane
R13085 T16516 T16515 arg1Of weights,Molecular
R13086 T16516 T16517 arg1Of weights,are
R13087 T16516 T16518 arg2Of weights,indicated
R13088 T16518 T16517 arg2Of indicated,are
R13089 T16518 T16519 arg1Of indicated,at
R13090 T16520 T16519 arg2Of right,at
R13091 T16522 T16521 arg1Of results,Similar
R13092 T16522 T16523 arg1Of results,were
R13093 T16522 T16524 arg2Of results,obtained
R13094 T16524 T16523 arg2Of obtained,were
R13095 T16524 T16525 arg1Of obtained,in
R13096 T16524 T16530 arg1Of obtained,in
R13097 T16524 T16534 arg1Of obtained,with
R13098 T16525 T16529 arg1Of in,and
R13099 T16528 T16525 arg2Of experiment,in
R13100 T16528 T16526 arg1Of experiment,a
R13101 T16528 T16527 arg1Of experiment,second
R13102 T16530 T16529 arg2Of in,and
R13103 T16533 T16530 arg2Of experiments,in
R13104 T16533 T16531 arg1Of experiments,two
R13105 T16533 T16532 arg1Of experiments,additional
R13106 T16538 T16534 arg2Of clone,with
R13107 T16538 T16535 arg1Of clone,a
R13108 T16538 T16536 arg1Of clone,second
R13109 T16538 T16537 arg1Of clone,HOIP-deficient
R13634 T17282 T17281 arg1Of recruitment,IKK
R13635 T17282 T17283 arg1Of recruitment,to
R13636 T17282 T17288 arg1Of recruitment,is
R13637 T17282 T17289 arg1Of recruitment,defective
R13638 T17287 T17283 arg2Of complex,to
R13639 T17287 T17284 arg1Of complex,the
R13640 T17287 T17285 arg1Of complex,CD40
R13641 T17287 T17286 arg1Of complex,signaling
R13642 T17288 T17290 arg1Of is,in
R13643 T17289 T17288 arg2Of defective,is
R13644 T17292 T17290 arg2Of cells,in
R13645 T17292 T17291 arg1Of cells,HOIP-deficient
R13646 T17294 T17293 arg2Of A,(
R13647 T17295 T17293 arg3Of ),(
R13648 T17298 T17293 arg1Of treatment,(
R13649 T17298 T17296 arg1Of treatment,SMAC
R13650 T17298 T17297 arg1Of treatment,peptide
R13651 T17298 T17299 arg1Of treatment,reduces
R13652 T17298 T17306 arg1Of treatment,may
R13653 T17298 T17307 arg1Of treatment,modify
R13654 T17299 T17305 arg1Of reduces,and
R13655 T17300 T17299 arg2Of recruitment,reduces
R13656 T17300 T17301 arg1Of recruitment,of
R13657 T17300 T17303 arg1Of recruitment,to
R13658 T17302 T17301 arg2Of cIAP1,of
R13659 T17304 T17303 arg2Of CD40,to
R13660 T17307 T17305 arg2Of modify,and
R13661 T17307 T17306 arg2Of modify,may
R13662 T17309 T17307 arg2Of recruitment,modify
R13663 T17309 T17308 arg1Of recruitment,HOIP
R13664 T17311 T17310 arg1Of cells,A20.2J
R13665 T17311 T17312 arg1Of cells,were
R13666 T17311 T17313 arg2Of cells,incubated
R13667 T17313 T17312 arg2Of incubated,were
R13668 T17313 T17314 arg1Of incubated,for
R13669 T17316 T17314 arg2Of hours,for
R13670 T17316 T17315 arg1Of hours,six
R13671 T17316 T17317 arg1Of hours,with
R13672 T17320 T17318 arg1Of peptide,membrane-permeable
R13673 T17320 T17319 arg1Of peptide,SMAC-N7
R13674 T17320 T17321 arg1Of peptide,or
R13675 T17321 T17317 arg2Of or,with
R13676 T17322 T17323 arg1Of 1.5,%
R13677 T17324 T17321 arg2Of DMSO,or
R13678 T17324 T17322 arg1Of DMSO,1.5
R13679 T17324 T17325 arg1Of DMSO,(
R13680 T17326 T17325 arg2Of solvent,(
R13681 T17326 T17327 arg2Of solvent,used
R13682 T17327 T17328 arg1Of used,for
R13683 T17330 T17328 arg2Of peptide,for
R13684 T17330 T17329 arg1Of peptide,the
R13685 T17331 T17325 arg3Of ),(
R13686 T17334 T17332 arg2Of incubation,Following
R13687 T17334 T17333 arg1Of incubation,the
R13688 T17337 T17336 arg1Of lysates,cell
R13689 T17337 T17338 arg1Of lysates,were
R13690 T17337 T17339 arg2Of lysates,prepared
R13691 T17337 T17341 arg2Of lysates,fractionated
R13692 T17337 T17346 arg2Of lysates,evaluated
R13693 T17339 T17340 arg1Of prepared,","
R13694 T17340 T17345 arg1Of ",",and
R13695 T17341 T17340 arg2Of fractionated,","
R13696 T17343 T17341 arg1Of SDS-PAGE,fractionated
R13697 T17343 T17342 arg2Of SDS-PAGE,by
R13698 T17345 T17332 arg1Of and,Following
R13699 T17345 T17335 arg1Of and,","
R13700 T17345 T17338 arg2Of and,were
R13701 T17345 T17344 arg1Of and,","
R13702 T17346 T17345 arg2Of evaluated,and
R13703 T17349 T17346 arg1Of blot,evaluated
R13704 T17349 T17347 arg2Of blot,by
R13705 T17349 T17348 arg1Of blot,Western
R13706 T17349 T17350 arg1Of blot,(
R13707 T17352 T17353 arg1Of 1,and
R13708 T17353 T17350 arg2Of and,(
R13709 T17353 T17351 arg1Of and,lanes
R13710 T17354 T17353 arg2Of 2,and
R13711 T17355 T17350 arg3Of ),(
R13712 T17356 T17357 arg2Of Cells,incubated
R13713 T17356 T17362 arg1Of Cells,were
R13714 T17356 T17364 arg2Of Cells,stimulated
R13715 T17357 T17358 arg1Of incubated,with
R13716 T17359 T17360 arg1Of DMSO,or
R13717 T17360 T17358 arg2Of or,with
R13718 T17361 T17360 arg2Of SMAC-N7,or
R13719 T17364 T17362 arg2Of stimulated,were
R13720 T17364 T17363 arg1Of stimulated,also
R13721 T17364 T17365 arg1Of stimulated,with
R13722 T17367 T17365 arg2Of beads,with
R13723 T17367 T17366 arg1Of beads,magnetic
R13724 T17367 T17368 arg2Of beads,coated
R13725 T17368 T17369 arg1Of coated,with
R13726 T17370 T17371 arg1Of anti-CD40,or
R13727 T17371 T17369 arg2Of or,with
R13728 T17375 T17371 arg2Of antibody,or
R13729 T17375 T17372 arg1Of antibody,an
R13730 T17375 T17373 arg1Of antibody,isotype
R13731 T17375 T17374 arg1Of antibody,control
R13732 T17378 T17377 arg2Of IP,(
R13733 T17379 T17377 arg3Of ),(
R13734 T17380 T17376 arg2Of material,Immunoprecipitated
R13735 T17380 T17377 arg1Of material,(
R13736 T17380 T17381 arg2Of material,bound
R13737 T17380 T17385 arg1Of material,was
R13738 T17380 T17386 arg2Of material,loaded
R13739 T17381 T17382 arg1Of bound,to
R13740 T17384 T17382 arg2Of beads,to
R13741 T17384 T17383 arg1Of beads,the
R13742 T17386 T17385 arg2Of loaded,was
R13743 T17386 T17387 arg1Of loaded,in
R13744 T17388 T17387 arg2Of lanes,in
R13745 T17388 T17389 arg1Of lanes,3-5
R13746 T17390 T17391 arg1Of Samples,of
R13747 T17390 T17397 arg1Of Samples,appear
R13748 T17394 T17391 arg2Of lysates,of
R13749 T17394 T17392 arg1Of lysates,the
R13750 T17394 T17393 arg1Of lysates,cell
R13751 T17394 T17395 arg1Of lysates,after
R13752 T17396 T17395 arg2Of immunoprecipitation,after
R13753 T17397 T17398 arg1Of appear,in
R13754 T17399 T17398 arg2Of lanes,in
R13755 T17399 T17400 arg1Of lanes,6–8
R13756 T17402 T17401 arg1Of blots,Western
R13757 T17402 T17403 arg1Of blots,were
R13758 T17402 T17404 arg2Of blots,probed
R13759 T17404 T17403 arg2Of probed,were
R13760 T17404 T17405 arg1Of probed,with
R13761 T17406 T17405 arg2Of antibodies,with
R13762 T17406 T17407 arg1Of antibodies,specific
R13763 T17407 T17408 arg1Of specific,for
R13764 T17409 T17410 arg1Of cIAP1,","
R13765 T17410 T17412 arg1Of ",",","
R13766 T17411 T17410 arg2Of TRAF2,","
R13767 T17412 T17415 arg1Of ",",and
R13768 T17413 T17412 arg2Of TRAF3,","
R13769 T17415 T17408 arg2Of and,for
R13770 T17415 T17414 arg1Of and,","
R13771 T17416 T17415 arg2Of HOIP,and
R13772 T17416 T17417 arg1Of HOIP,(
R13773 T17420 T17418 arg1Of weights,approximate
R13774 T17420 T17419 arg1Of weights,molecular
R13775 T17420 T17421 arg1Of weights,indicated
R13776 T17421 T17417 arg2Of indicated,(
R13777 T17421 T17422 arg1Of indicated,on
R13778 T17423 T17422 arg2Of left,on
R13779 T17424 T17417 arg3Of ),(
R13780 T17426 T17425 arg1Of results,Similar
R13781 T17426 T17427 arg1Of results,were
R13782 T17426 T17428 arg2Of results,obtained
R13783 T17428 T17427 arg2Of obtained,were
R13784 T17428 T17429 arg1Of obtained,in
R13785 T17432 T17429 arg2Of experiments.,in
R13786 T17432 T17430 arg1Of experiments.,two
R13787 T17432 T17431 arg1Of experiments.,additional
R13788 T17432 T17433 arg1Of experiments.,(
R13789 T17432 T17454 arg2Of experiments.,transduced
R13790 T17434 T17433 arg2Of B,(
R13791 T17435 T17433 arg3Of ),(
R13792 T17436 T17437 arg1Of CD40,was
R13793 T17436 T17438 arg2Of CD40,isolated
R13794 T17438 T17437 arg2Of isolated,was
R13795 T17438 T17439 arg1Of isolated,by
R13796 T17440 T17439 arg2Of immunoprecipitation,by
R13797 T17440 T17441 arg1Of immunoprecipitation,(
R13798 T17440 T17448 arg1Of immunoprecipitation,from
R13799 T17443 T17441 arg2Of in,(
R13800 T17443 T17442 arg1Of in,as
R13801 T17445 T17443 arg2Of A,in
R13802 T17445 T17444 arg2Of A,(
R13803 T17446 T17444 arg3Of ),(
R13804 T17447 T17441 arg3Of ),(
R13805 T17450 T17449 arg1Of cells,A20.2J
R13806 T17450 T17451 arg1Of cells,and
R13807 T17451 T17448 arg2Of and,from
R13808 T17453 T17451 arg2Of cells,and
R13809 T17453 T17452 arg1Of cells,HOIP-deficient
R13810 T17454 T17437 modOf transduced,was
R13811 T17454 T17455 arg1Of transduced,with
R13812 T17459 T17456 arg1Of vector,an
R13813 T17459 T17457 arg1Of vector,empty
R13814 T17459 T17458 arg1Of vector,retroviral
R13815 T17459 T17460 arg1Of vector,(
R13816 T17459 T17463 arg1Of vector,or
R13817 T17461 T17460 arg2Of pMIP,(
R13818 T17462 T17460 arg3Of ),(
R13819 T17463 T17455 arg2Of or,with
R13820 T17466 T17463 arg2Of vector,or
R13821 T17466 T17464 arg1Of vector,a
R13822 T17466 T17465 arg1Of vector,retroviral
R13823 T17466 T17467 arg1Of vector,encoding
R13824 T17468 T17467 arg2Of HOIP,encoding
R13825 T17469 T17470 arg1Of Material,immunoprecipitated
R13826 T17470 T17471 arg1Of immunoprecipitated,with
R13827 T17470 T17479 arg1Of immunoprecipitated,or
R13828 T17475 T17471 arg2Of antibody,with
R13829 T17475 T17472 arg1Of antibody,an
R13830 T17475 T17473 arg1Of antibody,isotype
R13831 T17475 T17474 arg1Of antibody,control
R13832 T17475 T17476 arg1Of antibody,(
R13833 T17477 T17476 arg2Of isotype,(
R13834 T17478 T17476 arg3Of ),(
R13835 T17481 T17480 arg1Of antibody,anti-CD40
R13836 T17481 T17482 arg1Of antibody,was
R13837 T17481 T17483 arg2Of antibody,examined
R13838 T17483 T17479 arg2Of examined,or
R13839 T17483 T17482 arg2Of examined,was
R13840 T17486 T17483 arg1Of blotting,examined
R13841 T17486 T17484 arg2Of blotting,by
R13842 T17486 T17485 arg1Of blotting,Western
R13843 T17486 T17487 arg1Of blotting,for
R13844 T17488 T17489 arg1Of CD40,","
R13845 T17489 T17491 arg1Of ",",","
R13846 T17490 T17489 arg2Of TRAF2,","
R13847 T17491 T17493 arg1Of ",",","
R13848 T17492 T17491 arg2Of TRAF3,","
R13849 T17493 T17495 arg1Of ",",","
R13850 T17494 T17493 arg2Of cIAP1,","
R13851 T17495 T17497 arg1Of ",",","
R13852 T17496 T17495 arg2Of HOIP,","
R13853 T17497 T17500 arg1Of ",",and
R13854 T17498 T17497 arg2Of IKKα/β,","
R13855 T17500 T17487 arg2Of and,for
R13856 T17500 T17499 arg1Of and,","
R13857 T17501 T17500 arg2Of IKKγ,and
R13858 T17501 T17502 arg1Of IKKγ,(
R13859 T17504 T17502 arg2Of panels,(
R13860 T17504 T17503 arg1Of panels,right
R13861 T17505 T17502 arg3Of ),(
R13862 T17507 T17506 arg1Of expression,HOIP
R13863 T17507 T17508 arg1Of expression,was
R13864 T17507 T17509 arg2Of expression,required
R13865 T17509 T17508 arg2Of required,was
R13866 T17509 T17510 arg1Of required,for
R13867 T17511 T17510 arg2Of coprecipitation,for
R13868 T17511 T17512 arg1Of coprecipitation,of
R13869 T17514 T17512 arg2Of proteins,of
R13870 T17514 T17513 arg1Of proteins,IKK
R13871 T17514 T17515 arg1Of proteins,with
R13872 T17516 T17515 arg2Of CD40,with
R13873 T17518 T17517 arg1Of lysates,Cell
R13874 T17518 T17519 arg1Of lysates,from
R13875 T17518 T17522 arg1Of lysates,are
R13876 T17518 T17523 arg2Of lysates,shown
R13877 T17521 T17519 arg2Of cells,from
R13878 T17521 T17520 arg1Of cells,unstimulated
R13879 T17523 T17522 arg2Of shown,are
R13880 T17523 T17524 arg1Of shown,in
R13881 T17527 T17524 arg2Of panels,in
R13882 T17527 T17525 arg1Of panels,the
R13883 T17527 T17526 arg1Of panels,left
R13884 T17529 T17528 arg1Of results,Similar
R13885 T17529 T17530 arg1Of results,were
R13886 T17529 T17531 arg2Of results,obtained
R13887 T17531 T17530 arg2Of obtained,were
R13888 T17531 T17532 arg1Of obtained,in
R13889 T17531 T17537 arg1Of obtained,in
R13890 T17531 T17540 arg1Of obtained,with
R13891 T17532 T17536 arg1Of in,and
R13892 T17535 T17532 arg2Of experiment,in
R13893 T17535 T17533 arg1Of experiment,a
R13894 T17535 T17534 arg1Of experiment,second
R13895 T17537 T17536 arg2Of in,and
R13896 T17539 T17537 arg2Of experiments,in
R13897 T17539 T17538 arg1Of experiments,two
R13898 T17544 T17540 arg2Of clone.,with
R13899 T17544 T17541 arg1Of clone.,a
R13900 T17544 T17542 arg1Of clone.,second
R13901 T17544 T17543 arg1Of clone.,HOIP-deficient
R13902 T17544 T17545 arg1Of clone.,(
R13903 T17544 T17548 modOf clone.,To
R13904 T17546 T17545 arg2Of C,(
R13905 T17547 T17545 arg3Of ),(
R13906 T17550 T17548 arg1Of evaluate,To
R13907 T17550 T17549 arg1Of evaluate,further
R13908 T17552 T17550 arg2Of recruitment,evaluate
R13909 T17552 T17551 arg1Of recruitment,HOIP-dependent
R13910 T17552 T17553 arg1Of recruitment,of
R13911 T17552 T17555 arg1Of recruitment,to
R13912 T17554 T17553 arg2Of IKKγ,of
R13913 T17556 T17555 arg2Of CD40,to
R13914 T17559 T17558 arg1Of cells,A20.2J
R13915 T17559 T17560 arg1Of cells,or
R13916 T17560 T17567 arg1Of or,were
R13917 T17560 T17568 arg2Of or,transduced
R13918 T17561 T17562 arg1Of HOIP-deficient,(
R13919 T17563 T17562 arg2Of HOIP-/-,(
R13920 T17564 T17562 arg3Of ),(
R13921 T17566 T17560 arg2Of cells,or
R13922 T17566 T17561 arg1Of cells,HOIP-deficient
R13923 T17566 T17565 arg1Of cells,A20.2J
R13924 T17568 T17530 modOf transduced,were
R13925 T17568 T17557 arg1Of transduced,","
R13926 T17568 T17567 arg2Of transduced,were
R13927 T17568 T17569 arg1Of transduced,with
R13928 T17573 T17570 arg1Of vector,an
R13929 T17573 T17571 arg1Of vector,empty
R13930 T17573 T17572 arg1Of vector,retroviral
R13931 T17573 T17574 arg1Of vector,or
R13932 T17574 T17569 arg2Of or,with
R13933 T17577 T17574 arg2Of construct,or
R13934 T17577 T17575 arg1Of construct,a
R13935 T17577 T17576 arg1Of construct,retroviral
R13936 T17577 T17578 arg1Of construct,encoding
R13937 T17581 T17578 arg2Of IKKγ,encoding
R13938 T17581 T17579 arg1Of IKKγ,BP
R13939 T17581 T17580 arg1Of IKKγ,epitope-tagged
R13940 T17581 T17582 arg1Of IKKγ,(
R13941 T17583 T17582 arg2Of noted,(
R13942 T17583 T17584 arg1Of noted,in
R13943 T17583 T17587 arg1Of noted,as
R13944 T17583 T17591 arg1Of noted,","
R13945 T17583 T17592 arg1Of noted,respectively
R13946 T17586 T17584 arg2Of figure,in
R13947 T17586 T17585 arg1Of figure,the
R13948 T17588 T17589 arg1Of pMIP,and
R13949 T17589 T17587 arg2Of and,as
R13950 T17590 T17589 arg2Of IKKγ,and
R13951 T17593 T17582 arg3Of ),(
R13952 T17594 T17595 arg1Of Lysates,(
R13953 T17594 T17599 arg1Of Lysates,and
R13954 T17597 T17595 arg2Of 1–3,(
R13955 T17597 T17596 arg1Of 1–3,lanes
R13956 T17598 T17595 arg3Of ),(
R13957 T17599 T17609 arg1Of and,from
R13958 T17599 T17613 arg1Of and,were
R13959 T17599 T17614 arg2Of and,fractionated
R13960 T17599 T17618 arg2Of and,evaluated
R13961 T17602 T17601 arg2Of IP,(
R13962 T17603 T17601 arg3Of ),(
R13963 T17604 T17599 arg2Of samples,and
R13964 T17604 T17600 arg1Of samples,immunoprecipitation
R13965 T17604 T17601 arg1Of samples,(
R13966 T17604 T17605 arg1Of samples,(
R13967 T17606 T17605 arg2Of lanes,(
R13968 T17606 T17607 arg1Of lanes,4–11
R13969 T17608 T17605 arg3Of ),(
R13970 T17612 T17609 arg2Of lines,from
R13971 T17612 T17610 arg1Of lines,the
R13972 T17612 T17611 arg1Of lines,cell
R13973 T17614 T17615 arg1Of fractionated,by
R13974 T17614 T17617 arg1Of fractionated,and
R13975 T17616 T17615 arg2Of SDS-PAGE,by
R13976 T17617 T17613 arg2Of and,were
R13977 T17617 T17622 arg1Of and,with
R13978 T17618 T17617 arg2Of evaluated,and
R13979 T17621 T17614 arg1Of blotting,fractionated
R13980 T17621 T17618 arg1Of blotting,evaluated
R13981 T17621 T17619 arg2Of blotting,by
R13982 T17621 T17620 arg1Of blotting,Western
R13983 T17623 T17622 arg2Of antibodies,with
R13984 T17623 T17624 arg1Of antibodies,to
R13985 T17627 T17626 arg1Of tag,BP
R13986 T17627 T17628 arg1Of tag,(
R13987 T17627 T17634 arg1Of tag,and
R13988 T17629 T17628 arg2Of IKKγ,(
R13989 T17629 T17630 arg1Of IKKγ,","
R13990 T17632 T17630 arg2Of panel,","
R13991 T17632 T17631 arg1Of panel,upper
R13992 T17633 T17628 arg3Of ),(
R13993 T17634 T17624 arg2Of and,to
R13994 T17634 T17625 arg1Of and,the
R13995 T17635 T17634 arg2Of TRAF2,and
R13996 T17639 T17636 arg1Of sample,The
R13997 T17639 T17637 arg1Of sample,anti-CD40
R13998 T17639 T17638 arg1Of sample,IP
R13999 T17639 T17640 arg1Of sample,in
R14000 T17639 T17643 arg1Of sample,was
R14001 T17639 T17644 arg2Of sample,treated
R14002 T17641 T17640 arg2Of lane,in
R14003 T17641 T17642 arg1Of lane,11
R14004 T17644 T17643 arg2Of treated,was
R14005 T17644 T17645 arg1Of treated,with
R14006 T17644 T17648 arg1Of treated,;
R14007 T17647 T17645 arg2Of phosphatase,with
R14008 T17647 T17646 arg1Of phosphatase,λ
R14009 T17650 T17649 arg1Of sample,the
R14010 T17650 T17651 arg1Of sample,in
R14011 T17650 T17654 arg1Of sample,was
R14012 T17650 T17655 arg1Of sample,mock-treated
R14013 T17652 T17651 arg2Of lane,in
R14014 T17652 T17653 arg1Of lane,10
R14015 T17654 T17648 arg2Of was,;
R14016 T17655 T17654 arg2Of mock-treated,was
R14017 T17657 T17656 arg1Of samples,Protein
R14018 T17657 T17665 arg1Of samples,were
R14019 T17657 T17667 arg2Of samples,fractionated
R14020 T17660 T17661 arg1Of those,treated
R14021 T17661 T17658 arg2Of treated,(
R14022 T17661 T17659 arg1Of treated,minus
R14023 T17661 T17662 arg1Of treated,with
R14024 T17663 T17662 arg2Of phosphatase,with
R14025 T17664 T17658 arg3Of ),(
R14026 T17667 T17658 arg1Of fractionated,(
R14027 T17667 T17665 arg2Of fractionated,were
R14028 T17667 T17666 arg1Of fractionated,also
R14029 T17667 T17668 arg1Of fractionated,on
R14030 T17667 T17677 arg1Of fractionated,for
R14031 T17671 T17668 arg2Of gel,on
R14032 T17671 T17669 arg1Of gel,a
R14033 T17671 T17670 arg1Of gel,separate
R14034 T17671 T17672 arg1Of gel,(
R14035 T17675 T17672 arg2Of concentration,(
R14036 T17675 T17673 arg1Of concentration,lower
R14037 T17675 T17674 arg1Of concentration,acrylamide
R14038 T17676 T17672 arg3Of ),(
R14039 T17679 T17677 arg2Of evaluation,for
R14040 T17679 T17678 arg1Of evaluation,the
R14041 T17679 T17680 arg1Of evaluation,of
R14042 T17681 T17682 arg1Of TRAF3,and
R14043 T17682 T17680 arg2Of and,of
R14044 T17682 T17684 arg1Of and,(
R14045 T17683 T17682 arg2Of HOIP,and
R14046 T17687 T17684 arg2Of panels,(
R14047 T17687 T17685 arg1Of panels,bottom
R14048 T17687 T17686 arg1Of panels,two
R14049 T17688 T17684 arg3Of ),(
R14050 T17690 T17689 arg1Of results,Similar
R14051 T17690 T17691 arg1Of results,were
R14052 T17690 T17692 arg2Of results,obtained
R14053 T17692 T17691 arg2Of obtained,were
R14054 T17692 T17693 arg1Of obtained,in
R14055 T17696 T17693 arg2Of experiments,in
R14056 T17696 T17694 arg1Of experiments,two
R14057 T17696 T17695 arg1Of experiments,additional
R11932 T15037 T15034 arg2Of sequence,of
R11933 T15037 T15035 arg1Of sequence,Rnf31

2_test

Id Subject Object Predicate Lexical cue
21829693-20614026-89653758 222-223 20614026 denotes 6
21829693-12958312-89653759 1038-1039 12958312 denotes 7
21829693-17878362-89653760 1043-1044 17878362 denotes 8
21829693-14981114-89653761 1048-1049 14981114 denotes 9
21829693-7681471-89653762 4077-4079 7681471 denotes 10
21829693-7681587-89653763 5461-5463 7681587 denotes 11
21829693-1701824-89653764 5467-5469 1701824 denotes 12
21829693-12949496-89653765 5951-5953 12949496 denotes 13
21829693-12958312-89653766 7946-7947 12958312 denotes 7
21829693-17878362-89653767 7951-7952 17878362 denotes 8
21829693-17633023-89653768 8072-8073 17633023 denotes 4
21829693-20614026-89653769 10748-10749 20614026 denotes 6
21829693-21455173-89653770 10887-10889 21455173 denotes 14
21829693-14532003-89653771 11117-11119 14532003 denotes 15
21829693-20614026-89653772 14849-14850 20614026 denotes 6
21829693-20614026-89653773 15647-15648 20614026 denotes 6
T45718 222-223 20614026 denotes 6
T92919 1038-1039 12958312 denotes 7
T70074 1043-1044 17878362 denotes 8
T10373 1048-1049 14981114 denotes 9
T72061 4077-4079 7681471 denotes 10
T18517 5461-5463 7681587 denotes 11
T73302 5467-5469 1701824 denotes 12
T513 5951-5953 12949496 denotes 13
T39552 7946-7947 12958312 denotes 7
T34158 7951-7952 17878362 denotes 8
T9898 8072-8073 17633023 denotes 4
T52587 10748-10749 20614026 denotes 6
T83759 10887-10889 21455173 denotes 14
T6761 11117-11119 14532003 denotes 15
T70732 14849-14850 20614026 denotes 6
T65429 15647-15648 20614026 denotes 6

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T2880 9-19 NNP denotes Generation
T2881 20-22 IN denotes of
T2882 23-37 NNP denotes HOIP-deficient
T2883 38-39 NNP denotes B
T2884 40-45 NNS denotes cells
T2885 46-49 IN denotes via
T2886 50-58 JJ denotes targeted
T2887 59-69 NN denotes disruption
T2888 70-72 IN denotes of
T2889 73-78 NNP denotes Rnf31
T2890 78-79 , denotes ,
T2891 80-83 DT denotes the
T2892 84-88 NN denotes gene
T2893 89-97 VBG denotes encoding
T2894 98-102 NNP denotes HOIP
T2895 103-105 IN denotes In
T2896 106-109 PRP$ denotes our
T2897 110-118 JJ denotes previous
T2898 119-124 NN denotes study
T2899 124-125 , denotes ,
T2900 126-128 PRP denotes we
T2901 129-141 VBD denotes demonstrated
T2902 142-146 IN denotes that
T2903 147-151 NNP denotes HOIP
T2904 152-154 VBZ denotes is
T2905 155-164 VBN denotes recruited
T2906 165-167 TO denotes to
T2907 168-171 DT denotes the
T2908 172-176 NNP denotes CD40
T2909 177-186 VBG denotes signaling
T2910 187-194 JJ denotes complex
T2911 195-197 IN denotes in
T2912 198-201 CD denotes two
T2913 202-207 NN denotes mouse
T2914 208-209 NNP denotes B
T2915 210-214 NN denotes cell
T2916 215-220 NNS denotes lines
T2917 221-222 VBP denotes [
T2918 222-223 CD denotes 6
T2919 223-224 NNP denotes ]
T2920 224-225 . denotes .
T2921 226-233 JJ denotes Similar
T2922 234-241 NNS denotes results
T2923 242-246 VBD denotes were
T2924 247-255 VBN denotes obtained
T2925 256-260 IN denotes with
T2926 261-266 NN denotes mouse
T2927 267-278 NNS denotes splenocytes
T2928 278-279 , denotes ,
T2929 280-290 VBG denotes indicating
T2930 291-295 IN denotes that
T2931 296-299 DT denotes the
T2932 300-311 NN denotes interaction
T2933 312-314 IN denotes of
T2934 315-319 CD denotes CD40
T2935 320-324 IN denotes with
T2936 325-329 NNP denotes HOIP
T2937 330-332 VBZ denotes is
T2938 333-336 RB denotes not
T2939 337-344 VBN denotes limited
T2940 345-347 TO denotes to
T2941 348-359 VBN denotes transformed
T2942 360-361 NNP denotes B
T2943 362-366 NN denotes cell
T2944 367-372 NNS denotes lines
T2945 373-374 -LRB- denotes (
T2946 374-385 JJ denotes unpublished
T2947 386-398 NNS denotes observations
T2948 398-399 -RRB- denotes )
T2949 399-400 . denotes .
T2950 401-403 PRP denotes We
T2951 404-408 RB denotes also
T2952 409-414 VBD denotes found
T2953 415-419 IN denotes that
T2954 420-423 DT denotes the
T2955 424-435 NN denotes recruitment
T2956 436-438 IN denotes of
T2957 439-443 NNP denotes HOIP
T2958 444-446 TO denotes to
T2959 447-451 NNP denotes CD40
T2960 452-455 VBD denotes was
T2961 456-471 JJ denotes TRAF2-dependent
T2962 472-475 CC denotes and
T2963 476-480 IN denotes that
T2964 481-495 NN denotes overexpression
T2965 496-498 IN denotes of
T2966 499-500 DT denotes a
T2967 501-510 VBN denotes truncated
T2968 511-515 NNP denotes HOIP
T2969 516-522 JJ denotes mutant
T2970 523-532 RB denotes partially
T2971 533-542 VBN denotes inhibited
T2972 543-556 JJ denotes CD40-mediated
T2973 557-562 NN denotes NF-κB
T2974 563-573 NN denotes activation
T2975 573-574 . denotes .
T2976 575-580 DT denotes These
T2977 581-588 NNS denotes results
T2978 589-596 VBP denotes support
T2979 597-600 DT denotes the
T2980 601-611 NN denotes hypothesis
T2981 612-616 WDT denotes that
T2982 617-621 NNP denotes HOIP
T2983 622-627 VBZ denotes plays
T2984 628-629 DT denotes a
T2985 630-634 NN denotes role
T2986 635-637 IN denotes in
T2987 638-642 CD denotes CD40
T2988 643-649 NN denotes signal
T2989 650-662 NN denotes transduction
T2990 662-663 . denotes .
T2991 664-666 TO denotes To
T2992 667-674 RBR denotes further
T2993 675-681 VB denotes define
T2994 682-685 DT denotes the
T2995 686-690 NN denotes role
T2996 691-694 CC denotes and
T2997 695-703 VB denotes evaluate
T2998 704-707 DT denotes the
T2999 708-718 NN denotes importance
T3000 719-721 IN denotes of
T3001 722-726 NNP denotes HOIP
T3002 727-729 IN denotes in
T3003 730-734 NNP denotes CD40
T3004 735-744 VBG denotes signaling
T3005 744-745 , denotes ,
T3006 746-748 PRP denotes we
T3007 749-753 VBD denotes used
T3008 754-761 JJ denotes somatic
T3009 762-766 NN denotes cell
T3010 767-771 NN denotes gene
T3011 772-781 VBG denotes targeting
T3012 782-784 TO denotes to
T3013 785-792 VB denotes disrupt
T3014 793-796 DT denotes the
T3015 797-801 NN denotes gene
T3016 802-810 VBG denotes encoding
T3017 811-815 NNP denotes HOIP
T3018 816-818 IN denotes in
T3019 819-822 DT denotes the
T3020 823-828 NN denotes mouse
T3021 829-830 NNP denotes B
T3022 831-835 NN denotes cell
T3023 836-840 NN denotes line
T3024 841-844 NNP denotes A20
T3025 844-846 CD denotes .2
T3026 846-847 NNP denotes J
T3027 847-848 . denotes .
T3028 849-853 DT denotes This
T3029 854-858 NN denotes cell
T3030 859-863 NN denotes line
T3031 864-867 VBZ denotes has
T3032 868-872 VBN denotes been
T3033 873-885 RB denotes particularly
T3034 886-892 JJ denotes useful
T3035 893-895 IN denotes in
T3036 896-899 DT denotes the
T3037 900-916 NN denotes characterization
T3038 917-919 IN denotes of
T3039 920-924 NNP denotes CD40
T3040 925-934 VBG denotes signaling
T3041 935-945 NNS denotes mechanisms
T3042 946-949 JJ denotes due
T3043 950-952 TO denotes to
T3044 953-956 DT denotes the
T3045 957-965 JJ denotes relative
T3046 966-970 NN denotes ease
T3047 971-975 IN denotes with
T3048 976-981 WDT denotes which
T3049 982-985 PRP$ denotes its
T3050 986-992 NN denotes genome
T3051 993-996 MD denotes can
T3052 997-999 VB denotes be
T3053 1000-1008 VBN denotes modified
T3054 1009-1011 IN denotes by
T3055 1012-1022 RB denotes homologous
T3056 1023-1036 NN denotes recombination
T3057 1037-1038 NN denotes [
T3058 1038-1039 CD denotes 7
T3059 1039-1040 NNP denotes ]
T3060 1040-1041 , denotes ,
T3061 1042-1043 NNP denotes [
T3062 1043-1044 CD denotes 8
T3063 1044-1045 NNP denotes ]
T3064 1045-1046 , denotes ,
T3065 1047-1048 NNP denotes [
T3066 1048-1049 CD denotes 9
T3067 1049-1050 NNP denotes ]
T3068 1050-1051 . denotes .
T3069 1052-1054 PRP denotes We
T3070 1055-1059 VBD denotes used
T3071 1060-1061 DT denotes a
T3072 1062-1071 VBG denotes targeting
T3073 1072-1078 NN denotes vector
T3074 1079-1086 JJ denotes capable
T3075 1087-1089 IN denotes of
T3076 1090-1100 VBG denotes undergoing
T3077 1101-1111 RB denotes homologous
T3078 1112-1125 NN denotes recombination
T3079 1126-1130 IN denotes with
T3080 1131-1136 NNP denotes Rnf31
T3081 1137-1138 -LRB- denotes (
T3082 1138-1141 DT denotes the
T3083 1142-1146 NN denotes gene
T3084 1147-1155 VBG denotes encoding
T3085 1156-1160 NNP denotes HOIP
T3086 1160-1161 -RRB- denotes )
T3087 1162-1164 TO denotes to
T3088 1165-1172 VB denotes disrupt
T3089 1173-1176 DT denotes the
T3090 1177-1183 NN denotes coding
T3091 1184-1192 NN denotes sequence
T3092 1193-1195 IN denotes of
T3093 1196-1199 DT denotes the
T3094 1200-1204 NN denotes gene
T3095 1205-1207 IN denotes in
T3096 1208-1212 NN denotes exon
T3097 1213-1214 CD denotes 5
T3098 1215-1216 -LRB- denotes (
T3099 1216-1220 NNP denotes Fig.
T3100 1221-1223 NNP denotes 1A
T3101 1223-1224 -RRB- denotes )
T3102 1224-1225 . denotes .
T3103 1226-1235 VBG denotes Following
T3104 1236-1248 NN denotes introduction
T3105 1249-1251 IN denotes of
T3106 1252-1255 DT denotes the
T3107 1256-1262 NN denotes vector
T3108 1262-1263 , denotes ,
T3109 1264-1267 DT denotes the
T3110 1268-1286 JJ denotes neomycin-resistant
T3111 1287-1293 NNS denotes clones
T3112 1294-1298 WDT denotes that
T3113 1299-1304 VBD denotes arose
T3114 1305-1309 VBD denotes were
T3115 1310-1318 VBN denotes screened
T3116 1319-1321 IN denotes by
T3117 1322-1325 NNP denotes PCR
T3118 1326-1339 NN denotes amplification
T3119 1340-1342 IN denotes of
T3120 1343-1350 JJ denotes genomic
T3121 1351-1354 NN denotes DNA
T3122 1355-1357 TO denotes to
T3123 1358-1366 VB denotes identify
T3124 1367-1372 NNS denotes cells
T3125 1373-1383 VBG denotes containing
T3126 1384-1385 DT denotes a
T3127 1386-1395 VBN denotes disrupted
T3128 1396-1401 NNP denotes Rnf31
T3129 1402-1408 NN denotes allele
T3130 1408-1409 . denotes .
T3131 1410-1412 TO denotes To
T3132 1413-1419 VB denotes remove
T3133 1420-1423 DT denotes the
T3134 1424-1434 JJ denotes selectable
T3135 1435-1441 NN denotes marker
T3136 1442-1446 NN denotes gene
T3137 1447-1455 NN denotes cassette
T3138 1456-1460 IN denotes from
T3139 1461-1464 DT denotes the
T3140 1465-1474 VBN denotes disrupted
T3141 1475-1480 NNP denotes Rnf31
T3142 1481-1487 NN denotes allele
T3143 1487-1488 , denotes ,
T3144 1489-1500 JJ denotes recombinant
T3145 1501-1505 NN denotes cell
T3146 1506-1511 NNS denotes lines
T3147 1512-1516 VBD denotes were
T3148 1517-1528 RB denotes transiently
T3149 1529-1540 VBN denotes transfected
T3150 1541-1545 IN denotes with
T3151 1546-1547 DT denotes a
T3152 1548-1555 NN denotes plasmid
T3153 1556-1560 WDT denotes that
T3154 1561-1568 VBZ denotes encodes
T3155 1569-1572 NNP denotes Cre
T3156 1573-1584 NN denotes recombinase
T3157 1584-1585 . denotes .
T3158 1586-1590 DT denotes This
T3159 1591-1595 NN denotes step
T3160 1596-1603 VBD denotes allowed
T3161 1604-1606 PRP denotes us
T3162 1607-1609 TO denotes to
T3163 1610-1617 VB denotes perform
T3164 1618-1619 DT denotes a
T3165 1620-1626 JJ denotes second
T3166 1627-1632 NN denotes round
T3167 1633-1635 IN denotes of
T3168 1636-1645 VBG denotes targeting
T3169 1646-1649 CC denotes and
T3170 1650-1654 NN denotes drug
T3171 1655-1664 NN denotes selection
T3172 1664-1665 , denotes ,
T3173 1666-1676 VBG denotes generating
T3174 1677-1682 NNS denotes cells
T3175 1683-1685 IN denotes in
T3176 1686-1691 WDT denotes which
T3177 1692-1696 DT denotes both
T3178 1697-1703 NNS denotes copies
T3179 1704-1706 IN denotes of
T3180 1707-1712 NNS denotes Rnf31
T3181 1713-1717 VBD denotes were
T3182 1718-1727 VBN denotes disrupted
T3183 1727-1728 . denotes .
T3184 1729-1732 CD denotes Two
T3185 1733-1744 JJ denotes independent
T3186 1745-1751 JJ denotes clonal
T3187 1752-1756 NN denotes cell
T3188 1757-1762 NNS denotes lines
T3189 1763-1767 VBD denotes were
T3190 1768-1774 VBN denotes chosen
T3191 1775-1778 IN denotes for
T3192 1779-1786 JJ denotes further
T3193 1787-1795 NN denotes analysis
T3194 1795-1796 . denotes .
T3195 1797-1801 NNP denotes HOIP
T3196 1802-1809 NN denotes protein
T3197 1810-1820 NN denotes expression
T3198 1821-1824 VBD denotes was
T3199 1825-1837 JJ denotes undetectable
T3200 1838-1840 IN denotes in
T3201 1841-1845 DT denotes both
T3202 1846-1850 NN denotes cell
T3203 1851-1856 NNS denotes lines
T3204 1856-1857 , denotes ,
T3205 1858-1860 IN denotes as
T3206 1861-1871 VBN denotes determined
T3207 1872-1874 IN denotes by
T3208 1875-1882 JJ denotes Western
T3209 1883-1887 NN denotes blot
T3210 1888-1896 NN denotes analysis
T3211 1897-1899 IN denotes of
T3212 1900-1904 NN denotes cell
T3213 1905-1912 NNS denotes lysates
T3214 1913-1914 -LRB- denotes (
T3215 1914-1918 NNP denotes Fig.
T3216 1919-1921 NNP denotes 1B
T3217 1921-1922 -RRB- denotes )
T3218 1922-1923 , denotes ,
T3219 1924-1937 VBG denotes demonstrating
T3220 1938-1942 IN denotes that
T3221 1943-1946 DT denotes the
T3222 1947-1956 VBG denotes targeting
T3223 1957-1964 NN denotes process
T3224 1965-1968 VBD denotes was
T3225 1969-1979 JJ denotes successful
T3226 1979-1980 . denotes .
T3227 1981-1983 IN denotes In
T3228 1984-1987 DT denotes the
T3229 1988-1992 NN denotes text
T3230 1993-1997 WDT denotes that
T3231 1998-2005 VBZ denotes follows
T3232 2005-2006 , denotes ,
T3233 2007-2010 DT denotes the
T3234 2011-2014 CD denotes two
T3235 2015-2028 JJ denotes gene-targeted
T3236 2029-2033 NN denotes cell
T3237 2034-2039 NNS denotes lines
T3238 2040-2043 VBP denotes are
T3239 2044-2052 VBN denotes referred
T3240 2053-2055 TO denotes to
T3241 2056-2058 IN denotes as
T3242 2059-2073 JJ denotes HOIP-deficient
T3243 2074-2079 NNS denotes cells
T3244 2079-2080 . denotes .
T3245 3361-3363 TO denotes To
T3246 3364-3370 VB denotes enable
T3247 3371-3373 PRP denotes us
T3248 3374-3376 TO denotes to
T3249 3377-3384 VB denotes confirm
T3250 3385-3389 IN denotes that
T3251 3390-3393 DT denotes any
T3252 3394-3403 VBG denotes signaling
T3253 3404-3406 CC denotes or
T3254 3407-3417 JJ denotes functional
T3255 3418-3425 NNS denotes defects
T3256 3426-3434 VBD denotes observed
T3257 3435-3437 IN denotes in
T3258 3438-3452 JJ denotes HOIP-deficient
T3259 3453-3458 NNS denotes cells
T3260 3459-3463 VBD denotes were
T3261 3464-3467 JJ denotes due
T3262 3468-3470 TO denotes to
T3263 3471-3481 NN denotes disruption
T3264 3482-3484 IN denotes of
T3265 3485-3488 DT denotes the
T3266 3489-3494 NNP denotes Rnf31
T3267 3495-3499 NN denotes gene
T3268 3499-3500 , denotes ,
T3269 3501-3505 DT denotes both
T3270 3506-3520 JJ denotes HOIP-deficient
T3271 3521-3525 NN denotes cell
T3272 3526-3531 NNS denotes lines
T3273 3532-3536 VBD denotes were
T3274 3537-3547 VBN denotes transduced
T3275 3548-3552 IN denotes with
T3276 3553-3554 DT denotes a
T3277 3555-3565 NN denotes retrovirus
T3278 3566-3574 VBG denotes encoding
T3279 3575-3586 JJ denotes FLAG-tagged
T3280 3587-3596 JJ denotes wild-type
T3281 3597-3601 NNP denotes HOIP
T3282 3601-3602 , denotes ,
T3283 3603-3607 RB denotes thus
T3284 3608-3617 VBG denotes restoring
T3285 3618-3622 NNP denotes HOIP
T3286 3623-3630 NN denotes protein
T3287 3631-3641 NN denotes expression
T3288 3642-3643 -LRB- denotes (
T3289 3643-3647 NNP denotes Fig.
T3290 3648-3650 NNP denotes 1B
T3291 3650-3651 -RRB- denotes )
T3292 3651-3652 . denotes .
T3293 3653-3658 DT denotes These
T3294 3659-3664 NNS denotes cells
T3295 3665-3668 VBP denotes are
T3296 3669-3677 VBN denotes referred
T3297 3678-3680 TO denotes to
T3298 3681-3683 IN denotes as
T3299 3684-3702 JJ denotes HOIP-reconstituted
T3300 3703-3708 NNS denotes cells
T3301 3708-3709 . denotes .
T3302 3710-3712 IN denotes In
T3303 3713-3716 DT denotes the
T3304 3717-3728 NNS denotes experiments
T3305 3729-3733 WDT denotes that
T3306 3734-3740 VBP denotes follow
T3307 3740-3741 , denotes ,
T3308 3742-3751 NNS denotes responses
T3309 3752-3754 IN denotes by
T3310 3755-3760 DT denotes these
T3311 3761-3766 NNS denotes cells
T3312 3767-3771 VBD denotes were
T3313 3772-3780 VBN denotes compared
T3314 3781-3783 TO denotes to
T3315 3784-3789 DT denotes those
T3316 3790-3792 IN denotes of
T3317 3793-3796 NNP denotes A20
T3318 3796-3798 CD denotes .2
T3319 3798-3799 NNP denotes J
T3320 3800-3805 NNS denotes cells
T3321 3806-3809 CC denotes and
T3322 3810-3824 JJ denotes HOIP-deficient
T3323 3825-3830 NNS denotes cells
T3324 3831-3841 VBN denotes transduced
T3325 3842-3846 IN denotes with
T3326 3847-3848 DT denotes a
T3327 3849-3859 JJ denotes retroviral
T3328 3860-3866 NN denotes vector
T3329 3867-3868 -LRB- denotes (
T3330 3868-3872 NNP denotes pMIP
T3331 3872-3873 -RRB- denotes )
T3332 3874-3881 VBG denotes lacking
T3333 3882-3883 DT denotes a
T3334 3884-3888 NNP denotes cDNA
T3335 3889-3895 NN denotes insert
T3336 3896-3897 -LRB- denotes (
T3337 3897-3902 JJ denotes empty
T3338 3903-3909 NN denotes vector
T3339 3909-3910 -RRB- denotes )
T3340 3910-3911 . denotes .
T4259 3913-3917 NNP denotes HOIP
T4260 3918-3920 VBZ denotes is
T4261 3921-3929 VBN denotes required
T4262 3930-3933 IN denotes for
T4263 3934-3947 JJ denotes CD40-mediated
T4264 3948-3952 NN denotes CD80
T4265 3953-3965 NN denotes upregulation
T4266 3966-3969 CC denotes and
T4267 3970-3980 NN denotes activation
T4268 3981-3983 IN denotes of
T4269 3984-3992 NN denotes germline
T4270 3993-4000 NN denotes epsilon
T4271 4001-4014 NN denotes transcription
T4272 4015-4025 NNP denotes Engagement
T4273 4026-4028 IN denotes of
T4274 4029-4033 NNP denotes CD40
T4275 4034-4036 IN denotes on
T4276 4037-4038 NNP denotes B
T4277 4039-4044 NNS denotes cells
T4278 4045-4056 VBZ denotes upregulates
T4279 4057-4067 NN denotes expression
T4280 4068-4070 IN denotes of
T4281 4071-4075 NNP denotes CD80
T4282 4076-4077 NNP denotes [
T4283 4077-4079 CD denotes 10
T4284 4079-4080 NNP denotes ]
T4285 4080-4081 , denotes ,
T4286 4082-4083 DT denotes a
T4287 4084-4088 NN denotes cell
T4288 4089-4096 NN denotes surface
T4289 4097-4104 NN denotes protein
T4290 4105-4109 WDT denotes that
T4291 4110-4118 VBZ denotes promotes
T4292 4119-4129 NN denotes activation
T4293 4130-4132 IN denotes of
T4294 4133-4134 NNP denotes T
T4295 4135-4140 NNS denotes cells
T4296 4141-4152 VBG denotes interacting
T4297 4153-4157 IN denotes with
T4298 4158-4159 NNP denotes B
T4299 4160-4165 NNS denotes cells
T4300 4166-4169 CC denotes and
T4301 4170-4175 JJ denotes other
T4302 4176-4179 NNP denotes APC
T4303 4179-4180 . denotes .
T4304 4181-4183 TO denotes To
T4305 4184-4189 VB denotes begin
T4306 4190-4200 VBG denotes evaluating
T4307 4201-4210 JJ denotes potential
T4308 4211-4224 NNS denotes contributions
T4309 4225-4227 IN denotes of
T4310 4228-4232 NNP denotes HOIP
T4311 4233-4235 TO denotes to
T4312 4236-4240 NNP denotes CD40
T4313 4241-4250 VBG denotes signaling
T4314 4250-4251 , denotes ,
T4315 4252-4254 PRP denotes we
T4316 4255-4262 VBD denotes assayed
T4317 4263-4266 DT denotes the
T4318 4267-4279 NN denotes upregulation
T4319 4280-4282 IN denotes of
T4320 4283-4287 CD denotes CD80
T4321 4288-4295 VBN denotes induced
T4322 4296-4298 IN denotes by
T4323 4299-4309 NN denotes engagement
T4324 4310-4312 IN denotes of
T4325 4313-4317 NNP denotes CD40
T4326 4318-4320 IN denotes on
T4327 4321-4329 JJ denotes parental
T4328 4330-4333 NNP denotes A20
T4329 4333-4335 CD denotes .2
T4330 4335-4336 NNP denotes J
T4331 4336-4337 , denotes ,
T4332 4338-4352 NNP denotes HOIP-deficient
T4333 4352-4353 , denotes ,
T4334 4354-4357 CC denotes and
T4335 4358-4376 JJ denotes HOIP-reconstituted
T4336 4377-4382 NNS denotes cells
T4337 4383-4384 -LRB- denotes (
T4338 4384-4388 JJ denotes Fig.
T4339 4389-4390 CD denotes 2
T4340 4390-4391 -RRB- denotes )
T4341 4391-4392 . denotes .
T4342 4393-4401 VBN denotes Compared
T4343 4402-4404 TO denotes to
T4344 4405-4408 DT denotes the
T4345 4409-4417 JJ denotes parental
T4346 4418-4422 NN denotes cell
T4347 4423-4427 NN denotes line
T4348 4427-4428 , denotes ,
T4349 4429-4432 DT denotes the
T4350 4433-4448 JJ denotes CD40-stimulated
T4351 4449-4461 NN denotes upregulation
T4352 4462-4464 IN denotes of
T4353 4465-4469 NN denotes CD80
T4354 4470-4473 VBD denotes was
T4355 4474-4486 RB denotes dramatically
T4356 4487-4494 VBN denotes reduced
T4357 4495-4497 IN denotes in
T4358 4498-4501 DT denotes all
T4359 4502-4516 JJ denotes HOIP-deficient
T4360 4517-4522 NNS denotes cells
T4361 4523-4529 VBN denotes tested
T4362 4529-4530 . denotes .
T4363 4531-4533 IN denotes In
T4364 4534-4542 NN denotes contrast
T4365 4542-4543 , denotes ,
T4366 4544-4556 NN denotes upregulation
T4367 4557-4559 IN denotes of
T4368 4560-4564 NN denotes CD80
T4369 4565-4568 VBD denotes was
T4370 4569-4577 VBN denotes restored
T4371 4578-4580 IN denotes in
T4372 4581-4599 JJ denotes HOIP-reconstituted
T4373 4600-4605 NNS denotes cells
T4374 4605-4606 , denotes ,
T4375 4607-4617 VBG denotes confirming
T4376 4618-4622 IN denotes that
T4377 4623-4626 DT denotes the
T4378 4627-4633 NN denotes defect
T4379 4634-4636 IN denotes in
T4380 4637-4641 NNP denotes CD80
T4381 4642-4654 NN denotes upregulation
T4382 4655-4657 IN denotes in
T4383 4658-4661 DT denotes the
T4384 4662-4676 JJ denotes HOIP-deficient
T4385 4677-4682 NNS denotes cells
T4386 4683-4686 VBD denotes was
T4387 4687-4690 JJ denotes due
T4388 4691-4693 TO denotes to
T4389 4694-4697 DT denotes the
T4390 4698-4708 NN denotes disruption
T4391 4709-4711 IN denotes of
T4392 4712-4717 NNP denotes Rnf31
T4393 4717-4718 . denotes .
T4394 5337-5341 CD denotes CD40
T4395 5342-5349 NNS denotes signals
T4396 5350-5352 IN denotes in
T4397 5353-5354 NNP denotes B
T4398 5355-5360 NNS denotes cells
T4399 5361-5364 MD denotes can
T4400 5365-5369 RB denotes also
T4401 5370-5380 VB denotes contribute
T4402 5381-5383 TO denotes to
T4403 5384-5387 DT denotes the
T4404 5388-5398 NN denotes activation
T4405 5399-5401 IN denotes of
T4406 5402-5405 NNP denotes DNA
T4407 5406-5419 NN denotes recombination
T4408 5420-5422 IN denotes in
T4409 5423-5426 DT denotes the
T4410 5427-5441 NN denotes immunoglobulin
T4411 5442-5447 JJ denotes heavy
T4412 5448-5453 NN denotes chain
T4413 5454-5459 NN denotes locus
T4414 5460-5461 NNP denotes [
T4415 5461-5463 CD denotes 11
T4416 5463-5464 NNP denotes ]
T4417 5464-5465 , denotes ,
T4418 5466-5467 NNP denotes [
T4419 5467-5469 CD denotes 12
T4420 5469-5470 NNP denotes ]
T4421 5470-5471 . denotes .
T4422 5472-5476 DT denotes This
T4423 5477-5484 NN denotes process
T4424 5484-5485 , denotes ,
T4425 5486-5491 VBN denotes known
T4426 5492-5494 IN denotes as
T4427 5495-5509 JJ denotes immunoglobulin
T4428 5510-5515 NN denotes class
T4429 5516-5525 NN denotes switching
T4430 5526-5527 -LRB- denotes (
T4431 5527-5529 CC denotes or
T4432 5530-5537 JJ denotes isotype
T4433 5538-5547 NN denotes switching
T4434 5547-5548 -RRB- denotes )
T4435 5548-5549 , denotes ,
T4436 5550-5556 VBZ denotes allows
T4437 5557-5558 NNP denotes B
T4438 5559-5564 NNS denotes cells
T4439 5564-5565 , denotes ,
T4440 5566-5575 RB denotes initially
T4441 5576-5586 VBG denotes expressing
T4442 5587-5590 NNP denotes IgM
T4443 5590-5591 , denotes ,
T4444 5592-5594 TO denotes to
T4445 5595-5601 VB denotes switch
T4446 5602-5604 TO denotes to
T4447 5605-5608 NNP denotes IgA
T4448 5608-5609 , denotes ,
T4449 5610-5613 NNP denotes IgG
T4450 5613-5614 , denotes ,
T4451 5615-5617 CC denotes or
T4452 5618-5621 NNP denotes IgE
T4453 5622-5631 VBG denotes depending
T4454 5632-5634 IN denotes on
T4455 5635-5638 DT denotes the
T4456 5639-5644 NN denotes class
T4457 5645-5647 IN denotes of
T4458 5648-5656 NN denotes antibody
T4459 5657-5661 RBS denotes most
T4460 5662-5673 JJ denotes appropriate
T4461 5674-5677 IN denotes for
T4462 5678-5679 DT denotes a
T4463 5680-5690 JJ denotes particular
T4464 5691-5697 JJ denotes immune
T4465 5698-5706 NN denotes response
T4466 5706-5707 . denotes .
T4467 5708-5717 VBG denotes Switching
T4468 5718-5720 TO denotes to
T4469 5721-5724 NNP denotes IgE
T4470 5725-5728 MD denotes can
T4471 5729-5731 VB denotes be
T4472 5732-5739 VBN denotes induced
T4473 5740-5742 IN denotes by
T4474 5743-5746 DT denotes the
T4475 5747-5758 NN denotes combination
T4476 5759-5761 IN denotes of
T4477 5762-5766 NN denotes IL-4
T4478 5767-5770 CC denotes and
T4479 5771-5775 JJ denotes CD40
T4480 5776-5783 NNS denotes signals
T4481 5783-5784 . denotes .
T4482 5785-5788 DT denotes The
T4483 5789-5793 NN denotes gene
T4484 5794-5807 NN denotes rearrangement
T4485 5808-5817 JJ denotes necessary
T4486 5818-5821 IN denotes for
T4487 5822-5825 JJ denotes IgE
T4488 5826-5836 NN denotes production
T4489 5837-5839 VBZ denotes is
T4490 5840-5848 VBN denotes preceded
T4491 5849-5851 IN denotes by
T4492 5852-5855 DT denotes the
T4493 5856-5866 NN denotes production
T4494 5867-5869 IN denotes of
T4495 5870-5880 JJ denotes non-coding
T4496 5881-5884 NNP denotes RNA
T4497 5885-5896 NNS denotes transcripts
T4498 5897-5901 IN denotes from
T4499 5902-5910 JJ denotes germline
T4500 5911-5920 NNS denotes sequences
T4501 5921-5923 IN denotes in
T4502 5924-5927 DT denotes the
T4503 5928-5931 NNP denotes IgE
T4504 5932-5937 NN denotes heavy
T4505 5938-5943 NN denotes chain
T4506 5944-5949 VBZ denotes locus
T4507 5950-5951 NNP denotes [
T4508 5951-5953 CD denotes 13
T4509 5953-5954 NNP denotes ]
T4510 5954-5955 . denotes .
T4511 5956-5958 TO denotes To
T4512 5959-5968 VB denotes determine
T4513 5969-5976 IN denotes whether
T4514 5977-5981 NNP denotes HOIP
T4515 5982-5984 VBZ denotes is
T4516 5985-5993 VBN denotes required
T4517 5994-5996 IN denotes in
T4518 5997-6000 DT denotes the
T4519 6001-6011 NN denotes initiation
T4520 6012-6014 IN denotes of
T4521 6015-6018 DT denotes the
T4522 6019-6024 NN denotes class
T4523 6025-6034 VBG denotes switching
T4524 6035-6042 NN denotes process
T4525 6042-6043 , denotes ,
T4526 6044-6047 DT denotes the
T4527 6048-6056 JJ denotes relevant
T4528 6057-6058 NNP denotes B
T4529 6059-6063 NN denotes cell
T4530 6064-6069 NNS denotes lines
T4531 6070-6074 VBD denotes were
T4532 6075-6085 VBN denotes stimulated
T4533 6086-6093 IN denotes through
T4534 6094-6098 NNP denotes CD40
T4535 6099-6101 IN denotes in
T4536 6102-6105 DT denotes the
T4537 6106-6114 NN denotes presence
T4538 6115-6117 IN denotes of
T4539 6118-6122 NN denotes IL-4
T4540 6123-6126 CC denotes and
T4541 6127-6133 VBN denotes tested
T4542 6134-6137 IN denotes for
T4543 6138-6148 NN denotes production
T4544 6149-6151 IN denotes of
T4545 6152-6160 NN denotes germline
T4546 6161-6168 NN denotes epsilon
T4547 6169-6170 -LRB- denotes (
T4548 6170-6173 NNP denotes GLε
T4549 6173-6174 -RRB- denotes )
T4550 6175-6186 NNS denotes transcripts
T4551 6187-6188 -LRB- denotes (
T4552 6188-6192 JJ denotes Fig.
T4553 6193-6194 CD denotes 3
T4554 6194-6195 -RRB- denotes )
T4555 6195-6196 . denotes .
T4556 6197-6206 JJ denotes Anti-CD40
T4557 6207-6215 NN denotes antibody
T4558 6216-6227 NN denotes stimulation
T4559 6228-6233 RB denotes alone
T4560 6234-6236 CC denotes or
T4561 6237-6245 RB denotes together
T4562 6246-6250 IN denotes with
T4563 6251-6255 NN denotes IL-4
T4564 6256-6263 VBD denotes induced
T4565 6264-6267 NNP denotes GLε
T4566 6268-6281 NN denotes transcription
T4567 6282-6284 IN denotes in
T4568 6285-6293 JJ denotes parental
T4569 6294-6297 NNP denotes A20
T4570 6297-6299 CD denotes .2
T4571 6299-6300 NNP denotes J
T4572 6301-6306 NNS denotes cells
T4573 6307-6309 IN denotes as
T4574 6310-6318 VBN denotes expected
T4575 6318-6319 . denotes .
T4576 6320-6322 IN denotes In
T4577 6323-6331 NN denotes contrast
T4578 6331-6332 , denotes ,
T4579 6333-6337 RB denotes only
T4580 6338-6341 JJ denotes low
T4581 6342-6348 NNS denotes levels
T4582 6349-6351 IN denotes of
T4583 6352-6355 NNP denotes GLε
T4584 6356-6366 NN denotes expression
T4585 6367-6371 VBD denotes were
T4586 6372-6380 VBN denotes detected
T4587 6381-6383 IN denotes in
T4588 6384-6398 JJ denotes HOIP-deficient
T4589 6399-6404 NNS denotes cells
T4590 6405-6415 VBN denotes stimulated
T4591 6416-6420 IN denotes with
T4592 6421-6427 DT denotes either
T4593 6428-6437 JJ denotes anti-CD40
T4594 6438-6446 NN denotes antibody
T4595 6447-6452 RB denotes alone
T4596 6453-6455 CC denotes or
T4597 6456-6465 JJ denotes anti-CD40
T4598 6466-6469 CC denotes and
T4599 6470-6474 NN denotes IL-4
T4600 6474-6475 . denotes .
T4601 6476-6482 JJ denotes Normal
T4602 6483-6489 NNS denotes levels
T4603 6490-6492 IN denotes of
T4604 6493-6496 NNP denotes GLε
T4605 6497-6507 NN denotes expression
T4606 6508-6512 VBD denotes were
T4607 6513-6520 VBN denotes induced
T4608 6521-6523 IN denotes in
T4609 6524-6542 JJ denotes HOIP-reconstituted
T4610 6543-6548 NNS denotes cells
T4611 6549-6559 VBN denotes stimulated
T4612 6560-6564 IN denotes with
T4613 6565-6574 JJ denotes anti-CD40
T4614 6575-6583 NN denotes antibody
T4615 6584-6589 RB denotes alone
T4616 6590-6592 CC denotes or
T4617 6593-6601 RB denotes together
T4618 6602-6606 IN denotes with
T4619 6607-6611 NN denotes IL-4
T4620 6611-6612 . denotes .
T4621 6613-6625 RB denotes Collectively
T4622 6625-6626 , denotes ,
T4623 6627-6632 DT denotes these
T4624 6633-6637 NNS denotes data
T4625 6638-6649 VBP denotes demonstrate
T4626 6650-6654 IN denotes that
T4627 6655-6659 NNP denotes HOIP
T4628 6660-6663 VBZ denotes has
T4629 6664-6665 DT denotes a
T4630 6666-6674 JJ denotes critical
T4631 6675-6679 NN denotes role
T4632 6680-6682 IN denotes in
T4633 6683-6691 JJ denotes cellular
T4634 6692-6701 NNS denotes responses
T4635 6702-6704 TO denotes to
T4636 6705-6709 CD denotes CD40
T4637 6710-6719 VBG denotes signaling
T4638 6719-6720 . denotes .
T5508 7584-7588 NNP denotes HOIP
T5509 7589-7597 VBZ denotes mediates
T5510 7598-7613 JJ denotes CD40-stimulated
T5511 7614-7619 NN denotes NF-κB
T5512 7620-7623 CC denotes and
T5513 7624-7627 NNP denotes JNK
T5514 7628-7638 NN denotes activation
T5515 7639-7642 DT denotes The
T5516 7643-7650 NNS denotes results
T5517 7651-7660 VBN denotes described
T5518 7661-7666 IN denotes above
T5519 7667-7671 NN denotes show
T5520 7672-7676 IN denotes that
T5521 7677-7681 NNP denotes HOIP
T5522 7682-7687 VBZ denotes plays
T5523 7688-7690 DT denotes an
T5524 7691-7700 JJ denotes important
T5525 7701-7705 NN denotes role
T5526 7706-7708 IN denotes in
T5527 7709-7722 JJ denotes CD40-mediated
T5528 7723-7731 NN denotes effector
T5529 7732-7741 NNS denotes functions
T5530 7742-7744 IN denotes of
T5531 7745-7746 NNP denotes B
T5532 7747-7752 NNS denotes cells
T5533 7752-7753 . denotes .
T5534 7754-7756 PRP denotes It
T5535 7757-7764 VBZ denotes follows
T5536 7765-7769 IN denotes that
T5537 7770-7774 NNP denotes HOIP
T5538 7775-7777 VBZ denotes is
T5539 7778-7784 JJ denotes likely
T5540 7785-7786 DT denotes a
T5541 7787-7790 JJ denotes key
T5542 7791-7799 NN denotes mediator
T5543 7800-7802 IN denotes of
T5544 7803-7807 NNP denotes CD40
T5545 7808-7817 VBG denotes signaling
T5546 7817-7818 . denotes .
T5547 7819-7821 TO denotes To
T5548 7822-7826 VB denotes test
T5549 7827-7831 DT denotes this
T5550 7832-7842 NN denotes hypothesis
T5551 7842-7843 , denotes ,
T5552 7844-7846 PRP denotes we
T5553 7847-7857 VBD denotes stimulated
T5554 7858-7861 NNP denotes A20
T5555 7861-7863 CD denotes .2
T5556 7863-7864 NNP denotes J
T5557 7865-7868 CC denotes and
T5558 7869-7883 JJ denotes HOIP-deficient
T5559 7884-7889 NNS denotes cells
T5560 7890-7894 IN denotes with
T5561 7895-7900 NNP denotes CD154
T5562 7901-7902 -LRB- denotes (
T5563 7902-7906 NNP denotes CD40
T5564 7907-7913 NN denotes ligand
T5565 7913-7914 -RRB- denotes )
T5566 7915-7924 VBN denotes expressed
T5567 7925-7927 IN denotes by
T5568 7928-7931 CD denotes HI5
T5569 7932-7938 JJ denotes insect
T5570 7939-7944 NNS denotes cells
T5571 7945-7946 VBP denotes [
T5572 7946-7947 CD denotes 7
T5573 7947-7948 NNP denotes ]
T5574 7948-7949 , denotes ,
T5575 7950-7951 NNP denotes [
T5576 7951-7952 CD denotes 8
T5577 7952-7953 NNP denotes ]
T5578 7954-7957 CC denotes and
T5579 7958-7966 VBN denotes measured
T5580 7967-7977 NN denotes activation
T5581 7978-7980 IN denotes of
T5582 7981-7984 DT denotes the
T5583 7985-7990 NNP denotes NF-κB
T5584 7991-7994 CC denotes and
T5585 7995-7998 NNP denotes JNK
T5586 7999-8007 NNS denotes pathways
T5587 8007-8008 , denotes ,
T5588 8009-8012 CD denotes two
T5589 8013-8015 IN denotes of
T5590 8016-8019 DT denotes the
T5591 8020-8025 JJ denotes major
T5592 8026-8041 JJ denotes transcriptional
T5593 8042-8052 NNS denotes regulators
T5594 8053-8062 VBN denotes activated
T5595 8063-8065 IN denotes by
T5596 8066-8070 NNP denotes CD40
T5597 8071-8072 NNP denotes [
T5598 8072-8073 CD denotes 4
T5599 8073-8074 NNP denotes ]
T5600 8074-8075 . denotes .
T5601 8076-8091 JJ denotes Cell-associated
T5602 8092-8097 NNP denotes CD154
T5603 8098-8101 VBD denotes was
T5604 8102-8106 VBN denotes used
T5605 8107-8109 IN denotes as
T5606 8110-8113 DT denotes the
T5607 8114-8122 NN denotes stimulus
T5608 8123-8125 IN denotes in
T5609 8126-8131 DT denotes these
T5610 8132-8143 NNS denotes experiments
T5611 8144-8146 IN denotes as
T5612 8147-8149 PRP denotes it
T5613 8150-8159 RB denotes typically
T5614 8160-8168 VBZ denotes provides
T5615 8169-8173 JJR denotes more
T5616 8174-8180 JJ denotes robust
T5617 8180-8181 , denotes ,
T5618 8182-8185 CC denotes and
T5619 8186-8195 RB denotes therefore
T5620 8196-8200 RBR denotes more
T5621 8201-8208 RB denotes readily
T5622 8209-8217 VBN denotes detected
T5623 8217-8218 , denotes ,
T5624 8219-8229 NN denotes activation
T5625 8230-8237 NNS denotes signals
T5626 8238-8242 IN denotes than
T5627 8243-8247 VBZ denotes does
T5628 8248-8257 JJ denotes anti-CD40
T5629 8258-8266 NN denotes antibody
T5630 8266-8267 . denotes .
T5631 8268-8278 NNP denotes Activation
T5632 8279-8281 IN denotes of
T5633 8282-8285 DT denotes the
T5634 8286-8295 JJ denotes canonical
T5635 8296-8301 NN denotes NF-κB
T5636 8302-8309 NN denotes pathway
T5637 8310-8312 VBZ denotes is
T5638 8313-8322 VBN denotes initiated
T5639 8323-8327 IN denotes with
T5640 8328-8331 DT denotes the
T5641 8332-8347 NN denotes phosphorylation
T5642 8348-8350 IN denotes of
T5643 8351-8354 NNP denotes IκB
T5644 8355-8363 NNS denotes proteins
T5645 8364-8366 IN denotes by
T5646 8367-8370 DT denotes the
T5647 8371-8374 NNP denotes IκB
T5648 8375-8381 NN denotes kinase
T5649 8382-8389 NN denotes complex
T5650 8390-8391 -LRB- denotes (
T5651 8391-8394 NNP denotes IKK
T5652 8394-8395 -RRB- denotes )
T5653 8395-8396 . denotes .
T5654 8397-8399 IN denotes In
T5655 8400-8407 VBG denotes resting
T5656 8408-8413 NNS denotes cells
T5657 8413-8414 , denotes ,
T5658 8415-8418 NNP denotes IκB
T5659 8419-8427 NNS denotes proteins
T5660 8428-8431 VBP denotes are
T5661 8432-8443 JJ denotes responsible
T5662 8444-8447 IN denotes for
T5663 8448-8460 NN denotes sequestering
T5664 8461-8466 NN denotes NF-κB
T5665 8467-8475 NNS denotes subunits
T5666 8476-8478 IN denotes in
T5667 8479-8482 DT denotes the
T5668 8483-8492 NN denotes cytoplasm
T5669 8492-8493 . denotes .
T5670 8494-8509 NN denotes Phosphorylation
T5671 8510-8512 IN denotes by
T5672 8513-8516 DT denotes the
T5673 8517-8520 NNP denotes IKK
T5674 8521-8528 JJ denotes complex
T5675 8529-8536 NNS denotes targets
T5676 8537-8540 VBP denotes IκB
T5677 8541-8549 NNS denotes proteins
T5678 8550-8553 IN denotes for
T5679 8554-8568 NN denotes ubiquitination
T5680 8569-8572 CC denotes and
T5681 8573-8584 NN denotes degradation
T5682 8584-8585 , denotes ,
T5683 8586-8594 VBG denotes allowing
T5684 8595-8600 NN denotes NF-κB
T5685 8601-8603 TO denotes to
T5686 8604-8609 VB denotes enter
T5687 8610-8613 DT denotes the
T5688 8614-8621 NN denotes nucleus
T5689 8622-8625 CC denotes and
T5690 8626-8634 VB denotes activate
T5691 8635-8639 NN denotes gene
T5692 8640-8650 NN denotes expression
T5693 8650-8651 . denotes .
T5694 8652-8665 JJ denotes CD40-mediated
T5695 8666-8681 NN denotes phosphorylation
T5696 8682-8685 CC denotes and
T5697 8686-8697 NN denotes degradation
T5698 8698-8700 IN denotes of
T5699 8701-8705 NNP denotes IκBα
T5700 8706-8708 IN denotes in
T5701 8709-8723 JJ denotes HOIP-deficient
T5702 8724-8729 NNS denotes cells
T5703 8730-8733 VBD denotes was
T5704 8734-8746 RB denotes dramatically
T5705 8747-8755 VBN denotes impaired
T5706 8756-8764 JJ denotes relative
T5707 8765-8767 TO denotes to
T5708 8768-8772 DT denotes that
T5709 8773-8781 VBN denotes observed
T5710 8782-8784 IN denotes in
T5711 8785-8793 JJ denotes parental
T5712 8794-8797 NNP denotes A20
T5713 8797-8799 CD denotes .2
T5714 8799-8800 NNP denotes J
T5715 8801-8806 NNS denotes cells
T5716 8807-8808 -LRB- denotes (
T5717 8808-8812 JJ denotes Fig.
T5718 8813-8814 CD denotes 4
T5719 8814-8815 -RRB- denotes )
T5720 8815-8816 . denotes .
T5721 8817-8819 PRP denotes We
T5722 8820-8824 RB denotes also
T5723 8825-8832 VBD denotes assayed
T5724 8833-8843 NN denotes activation
T5725 8844-8846 IN denotes of
T5726 8847-8850 DT denotes the
T5727 8851-8867 JJ denotes stress-activated
T5728 8868-8875 NN denotes protein
T5729 8876-8882 NN denotes kinase
T5730 8883-8886 NNP denotes JNK
T5731 8887-8889 IN denotes in
T5732 8890-8898 NN denotes response
T5733 8899-8901 TO denotes to
T5734 8902-8906 CD denotes CD40
T5735 8907-8917 NN denotes engagement
T5736 8918-8919 -LRB- denotes (
T5737 8919-8923 FW denotes Fig.
T5738 8924-8925 LS denotes 4
T5739 8925-8926 -RRB- denotes )
T5740 8926-8927 . denotes .
T5741 8928-8941 JJ denotes CD40-mediated
T5742 8942-8945 NNP denotes JNK
T5743 8946-8956 NN denotes activation
T5744 8957-8959 IN denotes in
T5745 8960-8974 JJ denotes HOIP-deficient
T5746 8975-8980 NNS denotes cells
T5747 8981-8984 VBD denotes was
T5748 8985-8993 VBN denotes impaired
T5749 8994-8996 IN denotes as
T5750 8997-9005 VBN denotes measured
T5751 9006-9008 IN denotes by
T5752 9009-9024 NN denotes phosphorylation
T5753 9025-9027 IN denotes of
T5754 9028-9034 NNP denotes Thr183
T5755 9035-9038 CC denotes and
T5756 9039-9045 NNP denotes Tyr185
T5757 9046-9048 IN denotes in
T5758 9049-9052 NNP denotes JNK
T5759 9052-9053 . denotes .
T5760 9054-9066 JJ denotes CD40-induced
T5761 9067-9077 NN denotes activation
T5762 9078-9080 IN denotes of
T5763 9081-9086 NNP denotes NF-κB
T5764 9087-9090 CC denotes and
T5765 9091-9094 NNP denotes JNK
T5766 9095-9097 IN denotes in
T5767 9098-9116 JJ denotes HOIP-reconstituted
T5768 9117-9122 NNS denotes cells
T5769 9123-9126 VBD denotes was
T5770 9127-9133 JJ denotes normal
T5771 9133-9134 , denotes ,
T5772 9135-9148 VBG denotes demonstrating
T5773 9149-9153 IN denotes that
T5774 9154-9157 DT denotes the
T5775 9158-9165 NNS denotes defects
T5776 9166-9174 VBD denotes observed
T5777 9175-9177 IN denotes in
T5778 9178-9192 JJ denotes gene-deficient
T5779 9193-9198 NNS denotes cells
T5780 9199-9203 VBD denotes were
T5781 9204-9207 JJ denotes due
T5782 9208-9210 TO denotes to
T5783 9211-9214 DT denotes the
T5784 9215-9222 NN denotes absence
T5785 9223-9225 IN denotes of
T5786 9226-9230 NNP denotes HOIP
T5787 9231-9241 NN denotes expression
T5788 9241-9242 . denotes .
T6738 8521-8528 NN denotes complex
T6739 10363-10367 IN denotes with
T6740 10368-10372 NNP denotes CD40
T6741 10373-10376 NNP denotes The
T6742 10377-10383 VBD denotes marked
T6743 10384-10391 NNS denotes defects
T6744 10392-10394 IN denotes in
T6745 10395-10408 JJ denotes CD40-mediated
T6746 10409-10413 NN denotes cell
T6747 10414-10424 NN denotes activation
T6748 10425-10428 CC denotes and
T6749 10429-10438 VBG denotes signaling
T6750 10439-10448 VBN denotes displayed
T6751 10449-10451 IN denotes by
T6752 10452-10466 JJ denotes HOIP-deficient
T6753 10467-10472 NNS denotes cells
T6754 10473-10482 VBD denotes suggested
T6755 10483-10487 IN denotes that
T6756 10488-10492 NNP denotes HOIP
T6757 10493-10501 VBZ denotes mediates
T6758 10502-10513 NN denotes recruitment
T6759 10514-10516 IN denotes of
T6760 10517-10525 JJ denotes critical
T6761 10526-10536 NNS denotes components
T6762 10537-10539 IN denotes of
T6763 10540-10543 DT denotes the
T6764 10544-10548 NNP denotes CD40
T6765 10549-10558 VBG denotes signaling
T6766 10559-10568 NN denotes apparatus
T6767 10569-10571 TO denotes to
T6768 10572-10575 DT denotes the
T6769 10576-10584 NN denotes receptor
T6770 10584-10585 . denotes .
T6771 10586-10596 RB denotes Previously
T6772 10596-10597 , denotes ,
T6773 10598-10600 PRP denotes we
T6774 10601-10613 VBD denotes demonstrated
T6775 10614-10618 IN denotes that
T6776 10619-10623 NNP denotes HOIP
T6777 10624-10626 VBZ denotes is
T6778 10627-10636 VBN denotes recruited
T6779 10637-10639 TO denotes to
T6780 10640-10643 DT denotes the
T6781 10644-10648 NNP denotes CD40
T6782 10649-10658 VBG denotes signaling
T6783 10659-10666 JJ denotes complex
T6784 10667-10669 IN denotes in
T6785 10670-10671 DT denotes a
T6786 10672-10687 JJ denotes TRAF2-dependent
T6787 10688-10694 NN denotes manner
T6788 10694-10695 , denotes ,
T6789 10696-10706 VBG denotes suggesting
T6790 10707-10711 IN denotes that
T6791 10712-10716 NNP denotes HOIP
T6792 10717-10726 NNS denotes functions
T6793 10727-10737 JJ denotes downstream
T6794 10738-10740 IN denotes of
T6795 10741-10746 CD denotes TRAF2
T6796 10747-10748 NN denotes [
T6797 10748-10749 CD denotes 6
T6798 10749-10750 NNP denotes ]
T6799 10750-10751 . denotes .
T6800 10752-10759 NNS denotes Studies
T6801 10760-10762 IN denotes by
T6802 10763-10769 NNS denotes others
T6803 10770-10777 VBP denotes suggest
T6804 10778-10782 IN denotes that
T6805 10783-10786 DT denotes the
T6806 10787-10803 JJ denotes TRAF2-associated
T6807 10804-10812 NNS denotes proteins
T6808 10813-10818 JJ denotes cIAP1
T6809 10819-10822 CC denotes and
T6810 10823-10828 JJ denotes cIAP2
T6811 10829-10833 NN denotes play
T6812 10834-10835 DT denotes a
T6813 10836-10840 NN denotes role
T6814 10841-10843 IN denotes in
T6815 10844-10847 DT denotes the
T6816 10848-10859 NN denotes recruitment
T6817 10860-10862 IN denotes of
T6818 10863-10867 NNP denotes HOIP
T6819 10868-10870 TO denotes to
T6820 10871-10876 CD denotes TNFR1
T6821 10877-10880 CC denotes and
T6822 10881-10885 CD denotes CD40
T6823 10886-10887 NNP denotes [
T6824 10887-10889 CD denotes 14
T6825 10889-10890 NNP denotes ]
T6826 10890-10891 . denotes .
T6827 10892-10901 RB denotes Therefore
T6828 10901-10902 , denotes ,
T6829 10903-10905 PRP denotes we
T6830 10906-10916 VBD denotes determined
T6831 10917-10924 IN denotes whether
T6832 10925-10928 DT denotes the
T6833 10929-10940 NN denotes association
T6834 10941-10943 IN denotes of
T6835 10944-10948 NNP denotes HOIP
T6836 10949-10953 IN denotes with
T6837 10954-10958 NNP denotes CD40
T6838 10959-10961 IN denotes in
T6839 10962-10965 NNP denotes A20
T6840 10965-10967 CD denotes .2
T6841 10967-10968 NNP denotes J
T6842 10969-10974 NNS denotes cells
T6843 10975-10978 VBD denotes was
T6844 10979-10986 VBN denotes altered
T6845 10987-10989 IN denotes by
T6846 10990-10999 NN denotes treatment
T6847 11000-11002 IN denotes of
T6848 11003-11008 NNS denotes cells
T6849 11009-11013 IN denotes with
T6850 11014-11016 DT denotes an
T6851 11017-11026 NN denotes inhibitor
T6852 11027-11029 IN denotes of
T6853 11030-11034 JJ denotes cIAP
T6854 11035-11043 NN denotes activity
T6855 11043-11044 , denotes ,
T6856 11045-11046 DT denotes a
T6857 11047-11065 JJ denotes membrane-permeable
T6858 11066-11073 NN denotes peptide
T6859 11074-11081 VBN denotes derived
T6860 11082-11086 IN denotes from
T6861 11087-11090 DT denotes the
T6862 11091-11100 NN denotes apoptosis
T6863 11101-11110 NN denotes regulator
T6864 11111-11115 NNP denotes SMAC
T6865 11116-11117 NNP denotes [
T6866 11117-11119 CD denotes 15
T6867 11119-11120 NNP denotes ]
T6868 11120-11121 . denotes .
T6869 11122-11124 PRP denotes We
T6870 11125-11130 VBD denotes found
T6871 11131-11135 IN denotes that
T6872 11136-11148 NN denotes pretreatment
T6873 11149-11151 IN denotes of
T6874 11152-11157 NNS denotes cells
T6875 11158-11162 IN denotes with
T6876 11163-11166 DT denotes the
T6877 11167-11171 NNP denotes SMAC
T6878 11172-11179 NN denotes peptide
T6879 11180-11192 RB denotes dramatically
T6880 11193-11200 VBD denotes reduced
T6881 11201-11204 DT denotes the
T6882 11205-11211 NN denotes amount
T6883 11212-11214 IN denotes of
T6884 11215-11220 JJ denotes cIAP1
T6885 11221-11231 VBN denotes associated
T6886 11232-11236 IN denotes with
T6887 11237-11240 DT denotes the
T6888 11241-11245 NNP denotes CD40
T6889 11246-11255 VBG denotes signaling
T6890 11256-11263 JJ denotes complex
T6891 11264-11266 IN denotes in
T6892 11267-11272 NNS denotes cells
T6893 11273-11283 VBN denotes stimulated
T6894 11284-11288 IN denotes with
T6895 11289-11298 JJ denotes anti-CD40
T6896 11299-11314 JJ denotes antibody-coated
T6897 11315-11320 NNS denotes beads
T6898 11321-11322 -LRB- denotes (
T6899 11322-11326 NNP denotes Fig.
T6900 11327-11329 NNP denotes 5A
T6901 11329-11330 -RRB- denotes )
T6902 11330-11331 . denotes .
T6903 11332-11336 NNP denotes SMAC
T6904 11337-11344 NN denotes peptide
T6905 11345-11354 NN denotes treatment
T6906 11355-11359 RB denotes also
T6907 11360-11368 VBD denotes resulted
T6908 11369-11371 IN denotes in
T6909 11372-11373 DT denotes a
T6910 11374-11380 JJ denotes slight
T6911 11381-11384 CC denotes but
T6912 11385-11397 JJ denotes reproducible
T6913 11398-11406 NN denotes decrease
T6914 11407-11409 IN denotes in
T6915 11410-11413 DT denotes the
T6916 11414-11420 NN denotes amount
T6917 11421-11423 IN denotes of
T6918 11424-11427 DT denotes the
T6919 11428-11433 JJ denotes major
T6920 11434-11438 NNP denotes HOIP
T6921 11439-11443 NN denotes form
T6922 11444-11453 VBD denotes recovered
T6923 11454-11456 IN denotes by
T6924 11457-11461 NNP denotes CD40
T6925 11462-11481 NN denotes immunoprecipitation
T6926 11481-11482 , denotes ,
T6927 11483-11488 IN denotes along
T6928 11489-11493 IN denotes with
T6929 11494-11496 DT denotes an
T6930 11497-11505 JJ denotes apparent
T6931 11506-11514 NN denotes increase
T6932 11515-11517 IN denotes in
T6933 11518-11524 JJR denotes higher
T6934 11525-11534 JJ denotes molecular
T6935 11535-11541 NN denotes weight
T6936 11542-11549 NNS denotes species
T6937 11550-11560 VBN denotes recognized
T6938 11561-11563 IN denotes by
T6939 11564-11573 JJ denotes anti-HOIP
T6940 11574-11582 NN denotes antibody
T6941 11582-11583 . denotes .
T6942 11584-11586 IN denotes In
T6943 11587-11595 NN denotes contrast
T6944 11595-11596 , denotes ,
T6945 11597-11606 NN denotes treatment
T6946 11607-11611 IN denotes with
T6947 11612-11615 DT denotes the
T6948 11616-11620 NNP denotes SMAC
T6949 11621-11628 NN denotes peptide
T6950 11629-11632 VBD denotes did
T6951 11633-11636 RB denotes not
T6952 11637-11642 VB denotes alter
T6953 11643-11646 DT denotes the
T6954 11647-11653 NN denotes amount
T6955 11654-11656 CC denotes or
T6956 11657-11666 JJ denotes molecular
T6957 11667-11673 NN denotes weight
T6958 11674-11676 IN denotes of
T6959 11677-11681 NNP denotes HOIP
T6960 11682-11689 NN denotes present
T6961 11690-11692 IN denotes in
T6962 11693-11697 NN denotes cell
T6963 11698-11705 NNS denotes lysates
T6964 11705-11706 . denotes .
T6965 11707-11712 DT denotes These
T6966 11713-11717 NNS denotes data
T6967 11718-11725 VBP denotes suggest
T6968 11726-11730 IN denotes that
T6969 11731-11735 NNP denotes SMAC
T6970 11736-11743 NN denotes peptide
T6971 11744-11753 NN denotes treatment
T6972 11754-11766 RB denotes specifically
T6973 11767-11773 VBZ denotes alters
T6974 11774-11777 DT denotes the
T6975 11778-11793 NNS denotes characteristics
T6976 11794-11796 IN denotes of
T6977 11797-11812 JJ denotes CD40-associated
T6978 11813-11817 NNP denotes HOIP
T6979 11818-11824 RB denotes rather
T6980 11825-11829 IN denotes than
T6981 11830-11833 DT denotes the
T6982 11834-11840 JJ denotes entire
T6983 11841-11849 JJ denotes cellular
T6984 11850-11854 NN denotes pool
T6985 11855-11857 IN denotes of
T6986 11858-11862 DT denotes this
T6987 11863-11870 NN denotes protein
T6988 11870-11871 . denotes .
T6989 11872-11880 RB denotes Together
T6990 11880-11881 , denotes ,
T6991 11882-11887 DT denotes these
T6992 11888-11895 NNS denotes results
T6993 11896-11903 VBP denotes support
T6994 11904-11907 DT denotes the
T6995 11908-11912 NN denotes idea
T6996 11913-11917 IN denotes that
T6997 11918-11921 DT denotes the
T6998 11922-11926 NNP denotes cIAP
T6999 11927-11935 NNS denotes proteins
T7000 11936-11945 VBP denotes influence
T7001 11946-11949 DT denotes the
T7002 11950-11961 NN denotes recruitment
T7003 11962-11965 CC denotes and
T7004 11966-11984 JJ denotes post-translational
T7005 11985-11997 NN denotes modification
T7006 11998-12003 NN denotes state
T7007 12004-12006 IN denotes of
T7008 12007-12022 JJ denotes CD40-associated
T7009 12023-12027 NNP denotes HOIP
T7010 12027-12028 . denotes .
T7011 14482-14484 TO denotes To
T7012 14485-14489 VB denotes test
T7013 14490-14493 DT denotes the
T7014 14494-14505 NN denotes possibility
T7015 14506-14510 WDT denotes that
T7016 14511-14515 NNP denotes HOIP
T7017 14516-14518 VBZ denotes is
T7018 14519-14530 JJ denotes responsible
T7019 14531-14534 IN denotes for
T7020 14535-14538 DT denotes the
T7021 14539-14550 NN denotes recruitment
T7022 14551-14553 IN denotes of
T7023 14554-14559 JJ denotes other
T7024 14560-14568 JJ denotes critical
T7025 14569-14578 VBG denotes signaling
T7026 14579-14587 NNS denotes proteins
T7027 14588-14590 TO denotes to
T7028 14591-14595 NNP denotes CD40
T7029 14595-14596 , denotes ,
T7030 14597-14599 PRP denotes we
T7031 14600-14618 VBD denotes immunoprecipitated
T7032 14619-14623 NNP denotes CD40
T7033 14624-14633 VBG denotes signaling
T7034 14634-14643 NNS denotes complexes
T7035 14644-14648 IN denotes from
T7036 14649-14663 JJ denotes HOIP-deficient
T7037 14664-14667 CC denotes and
T7038 14668-14686 JJ denotes HOIP-reconstituted
T7039 14687-14692 NNS denotes cells
T7040 14693-14694 -LRB- denotes (
T7041 14694-14698 NNP denotes Fig.
T7042 14699-14701 NNP denotes 5B
T7043 14701-14702 -RRB- denotes )
T7044 14702-14703 . denotes .
T7045 14704-14706 IN denotes As
T7046 14707-14716 VBN denotes described
T7047 14717-14727 RB denotes previously
T7048 14727-14728 , denotes ,
T7049 14729-14733 NNP denotes CD40
T7050 14734-14743 VBG denotes signaling
T7051 14744-14753 NNS denotes complexes
T7052 14754-14772 VBN denotes immunoprecipitated
T7053 14773-14777 IN denotes from
T7054 14778-14781 CD denotes A20
T7055 14781-14783 CD denotes .2
T7056 14783-14784 NNP denotes J
T7057 14785-14790 NNS denotes cells
T7058 14791-14800 VBD denotes contained
T7059 14801-14805 NNP denotes HOIP
T7060 14806-14808 RB denotes as
T7061 14809-14813 RB denotes well
T7062 14814-14816 IN denotes as
T7063 14817-14822 CD denotes TRAF2
T7064 14822-14823 , denotes ,
T7065 14824-14829 CD denotes TRAF3
T7066 14829-14830 , denotes ,
T7067 14831-14835 NNP denotes IKKα
T7068 14835-14836 NNP denotes /
T7069 14836-14837 NN denotes β
T7070 14837-14838 , denotes ,
T7071 14839-14842 CC denotes and
T7072 14843-14847 NNP denotes IKKγ
T7073 14848-14849 NNP denotes [
T7074 14849-14850 CD denotes 6
T7075 14850-14851 NNP denotes ]
T7076 14851-14852 . denotes .
T7077 14853-14856 DT denotes The
T7078 14857-14864 NNS denotes amounts
T7079 14865-14867 IN denotes of
T7080 14868-14873 CD denotes TRAF2
T7081 14873-14874 , denotes ,
T7082 14875-14880 CD denotes TRAF3
T7083 14880-14881 , denotes ,
T7084 14882-14885 CC denotes and
T7085 14886-14891 NNP denotes cIAP1
T7086 14892-14894 IN denotes in
T7087 14895-14899 NNP denotes CD40
T7088 14900-14918 VBZ denotes immunoprecipitates
T7089 14919-14923 IN denotes from
T7090 14924-14938 JJ denotes HOIP-deficient
T7091 14939-14944 NNS denotes cells
T7092 14945-14949 VBD denotes were
T7093 14950-14957 JJ denotes similar
T7094 14958-14960 TO denotes to
T7095 14961-14966 DT denotes those
T7096 14967-14971 IN denotes from
T7097 14972-14980 JJ denotes parental
T7098 14981-14984 NNP denotes A20
T7099 14984-14986 CD denotes .2
T7100 14986-14987 NNP denotes J
T7101 14988-14993 NNS denotes cells
T7102 14993-14994 , denotes ,
T7103 14995-15005 VBG denotes indicating
T7104 15006-15010 IN denotes that
T7105 15011-15015 NNP denotes HOIP
T7106 15016-15018 VBZ denotes is
T7107 15019-15022 RB denotes not
T7108 15023-15031 VBN denotes required
T7109 15032-15035 IN denotes for
T7110 15036-15039 DT denotes the
T7111 15040-15051 NN denotes association
T7112 15052-15054 IN denotes of
T7113 15055-15060 DT denotes these
T7114 15061-15069 NNS denotes proteins
T7115 15070-15074 IN denotes with
T7116 15075-15079 NNP denotes CD40
T7117 15079-15080 . denotes .
T7118 15081-15083 IN denotes In
T7119 15084-15092 NN denotes contrast
T7120 15092-15093 , denotes ,
T7121 15094-15098 NNP denotes IKKα
T7122 15098-15099 NNP denotes /
T7123 15099-15100 NN denotes β
T7124 15101-15104 CC denotes and
T7125 15105-15109 NNP denotes IKKγ
T7126 15110-15114 VBD denotes were
T7127 15115-15118 RB denotes not
T7128 15119-15129 JJ denotes detectable
T7129 15130-15132 IN denotes in
T7130 15133-15137 CD denotes CD40
T7131 15138-15156 NNS denotes immunoprecipitates
T7132 15157-15166 VBD denotes recovered
T7133 15167-15171 IN denotes from
T7134 15172-15186 JJ denotes HOIP-deficient
T7135 15187-15192 NNS denotes cells
T7136 15193-15194 -LRB- denotes (
T7137 15194-15198 NNP denotes Fig.
T7138 15199-15201 NNP denotes 5B
T7139 15201-15202 , denotes ,
T7140 15202-15203 NNP denotes C
T7141 15203-15204 -RRB- denotes )
T7142 15204-15205 . denotes .
T7143 15206-15209 DT denotes The
T7144 15210-15217 NNS denotes amounts
T7145 15218-15220 IN denotes of
T7146 15221-15225 NNP denotes IKKα
T7147 15225-15226 NNP denotes /
T7148 15226-15227 NN denotes β
T7149 15228-15231 CC denotes and
T7150 15232-15236 NNP denotes IKKγ
T7151 15237-15239 IN denotes in
T7152 15240-15244 NNP denotes CD40
T7153 15245-15263 VBZ denotes immunoprecipitates
T7154 15264-15268 IN denotes from
T7155 15269-15287 JJ denotes HOIP-reconstituted
T7156 15288-15293 NNS denotes cells
T7157 15294-15298 VBD denotes were
T7158 15299-15306 JJ denotes similar
T7159 15307-15309 TO denotes to
T7160 15310-15315 DT denotes those
T7161 15316-15324 VBN denotes detected
T7162 15325-15327 IN denotes in
T7163 15328-15335 NNS denotes samples
T7164 15336-15344 VBN denotes prepared
T7165 15345-15349 IN denotes from
T7166 15350-15358 JJ denotes parental
T7167 15359-15364 NNS denotes cells
T7168 15364-15365 , denotes ,
T7169 15366-15379 VBG denotes demonstrating
T7170 15380-15384 IN denotes that
T7171 15385-15388 DT denotes the
T7172 15389-15396 NNS denotes defects
T7173 15397-15399 IN denotes in
T7174 15400-15403 NNP denotes IKK
T7175 15404-15415 NN denotes recruitment
T7176 15416-15418 PRP denotes we
T7177 15419-15427 VBD denotes observed
T7178 15428-15432 VBD denotes were
T7179 15433-15436 JJ denotes due
T7180 15437-15449 RB denotes specifically
T7181 15450-15452 TO denotes to
T7182 15453-15456 DT denotes the
T7183 15457-15464 NN denotes absence
T7184 15465-15467 IN denotes of
T7185 15468-15472 NNP denotes HOIP
T7186 15473-15483 NN denotes expression
T7187 15483-15484 . denotes .
T7188 15485-15490 DT denotes These
T7189 15491-15495 NNS denotes data
T7190 15496-15504 VBP denotes indicate
T7191 15505-15509 IN denotes that
T7192 15510-15514 NNP denotes HOIP
T7193 15515-15517 VBZ denotes is
T7194 15518-15526 VBN denotes required
T7195 15527-15530 IN denotes for
T7196 15531-15542 NN denotes recruitment
T7197 15543-15545 IN denotes of
T7198 15546-15549 DT denotes the
T7199 15550-15566 JJ denotes NF-κB-activating
T7200 15567-15574 NN denotes complex
T7201 15575-15577 TO denotes to
T7202 15578-15581 DT denotes the
T7203 15582-15586 NNP denotes CD40
T7204 15587-15596 VBG denotes signaling
T7205 15597-15604 NN denotes complex
T7206 15604-15605 . denotes .
T7207 15606-15608 IN denotes As
T7208 15609-15614 VBN denotes shown
T7209 15615-15619 RB denotes here
T7210 15620-15623 CC denotes and
T7211 15624-15626 IN denotes in
T7212 15627-15630 PRP$ denotes our
T7213 15631-15639 JJ denotes previous
T7214 15640-15645 NN denotes study
T7215 15646-15647 NN denotes [
T7216 15647-15648 CD denotes 6
T7217 15648-15649 NNP denotes ]
T7218 15649-15650 , denotes ,
T7219 15651-15655 NNP denotes IKKγ
T7220 15656-15663 NN denotes present
T7221 15664-15666 IN denotes in
T7222 15667-15671 NNP denotes CD40
T7223 15672-15690 VBZ denotes immunoprecipitates
T7224 15691-15698 VBZ denotes appears
T7225 15699-15701 TO denotes to
T7226 15702-15706 VB denotes have
T7227 15707-15708 DT denotes a
T7228 15709-15715 JJR denotes higher
T7229 15716-15725 JJ denotes molecular
T7230 15726-15732 NN denotes weight
T7231 15733-15737 IN denotes than
T7232 15738-15742 DT denotes that
T7233 15743-15748 VBN denotes found
T7234 15749-15751 IN denotes in
T7235 15752-15756 NN denotes cell
T7236 15757-15764 NNS denotes lysates
T7237 15764-15765 . denotes .
T7238 15766-15768 TO denotes To
T7239 15769-15776 VB denotes confirm
T7240 15777-15781 IN denotes that
T7241 15782-15786 DT denotes this
T7242 15787-15793 JJR denotes higher
T7243 15794-15803 JJ denotes molecular
T7244 15804-15810 NN denotes weight
T7245 15811-15818 NNS denotes species
T7246 15819-15822 VBD denotes was
T7247 15823-15829 RB denotes indeed
T7248 15830-15834 NNP denotes IKKγ
T7249 15834-15835 , denotes ,
T7250 15836-15838 PRP denotes we
T7251 15839-15848 VBD denotes generated
T7252 15849-15852 NNP denotes A20
T7253 15852-15854 CD denotes .2
T7254 15854-15855 NNP denotes J
T7255 15856-15860 NN denotes cell
T7256 15861-15866 NNS denotes lines
T7257 15867-15877 VBG denotes containing
T7258 15878-15879 DT denotes a
T7259 15880-15886 RB denotes stably
T7260 15887-15897 VBN denotes integrated
T7261 15898-15908 JJ denotes retroviral
T7262 15909-15915 NN denotes vector
T7263 15916-15920 WDT denotes that
T7264 15921-15928 VBD denotes encoded
T7265 15929-15931 DT denotes an
T7266 15932-15946 JJ denotes epitope-tagged
T7267 15947-15954 NN denotes version
T7268 15955-15957 IN denotes of
T7269 15958-15963 NN denotes mouse
T7270 15964-15968 NNP denotes IKKγ
T7271 15968-15969 . denotes .
T7272 15970-15974 NN denotes Cell
T7273 15975-15982 NNS denotes lysates
T7274 15983-15986 CC denotes and
T7275 15987-15991 JJ denotes CD40
T7276 15992-16010 NNS denotes immunoprecipitates
T7277 16011-16019 VBN denotes prepared
T7278 16020-16024 IN denotes from
T7279 16025-16030 DT denotes these
T7280 16031-16036 NNS denotes cells
T7281 16037-16041 VBD denotes were
T7282 16042-16050 VBN denotes analyzed
T7283 16051-16053 IN denotes by
T7284 16054-16061 JJ denotes Western
T7285 16062-16070 VBG denotes blotting
T7286 16071-16074 IN denotes for
T7287 16075-16078 DT denotes the
T7288 16079-16086 NN denotes epitope
T7289 16087-16090 NN denotes tag
T7290 16091-16092 -LRB- denotes (
T7291 16092-16096 NNP denotes Fig.
T7292 16097-16099 NNP denotes 5C
T7293 16099-16100 -RRB- denotes )
T7294 16100-16101 . denotes .
T7295 16102-16106 DT denotes This
T7296 16107-16115 NN denotes analysis
T7297 16116-16124 VBD denotes produced
T7298 16125-16126 DT denotes a
T7299 16127-16134 NN denotes pattern
T7300 16135-16137 IN denotes of
T7301 16138-16143 NNS denotes bands
T7302 16144-16148 WDT denotes that
T7303 16149-16152 VBD denotes was
T7304 16153-16164 RB denotes essentially
T7305 16165-16168 DT denotes the
T7306 16169-16173 JJ denotes same
T7307 16174-16176 IN denotes as
T7308 16177-16181 DT denotes that
T7309 16182-16190 VBN denotes obtained
T7310 16191-16196 VBG denotes using
T7311 16197-16199 DT denotes an
T7312 16200-16208 NN denotes antibody
T7313 16209-16217 NN denotes specific
T7314 16218-16221 IN denotes for
T7315 16222-16228 JJ denotes native
T7316 16229-16233 NNP denotes IKKγ
T7317 16234-16235 -LRB- denotes (
T7318 16235-16242 VB denotes compare
T7319 16243-16249 NNS denotes panels
T7320 16250-16251 NNP denotes B
T7321 16252-16255 CC denotes and
T7322 16256-16257 NNP denotes C
T7323 16258-16260 IN denotes in
T7324 16261-16265 NNP denotes Fig.
T7325 16266-16267 CD denotes 5
T7326 16267-16268 -RRB- denotes )
T7327 16268-16269 . denotes .
T7328 16270-16278 RB denotes Moreover
T7329 16278-16279 , denotes ,
T7330 16280-16288 NN denotes analysis
T7331 16289-16291 IN denotes of
T7332 16292-16297 NNS denotes cells
T7333 16298-16308 VBG denotes expressing
T7334 16309-16316 NN denotes epitope
T7335 16317-16328 NN denotes tagged-IKKγ
T7336 16329-16338 VBD denotes confirmed
T7337 16339-16343 IN denotes that
T7338 16344-16355 NN denotes recruitment
T7339 16356-16358 IN denotes of
T7340 16359-16363 NNP denotes IKKγ
T7341 16364-16366 TO denotes to
T7342 16367-16371 NNP denotes CD40
T7343 16372-16380 VBZ denotes requires
T7344 16381-16385 NNP denotes HOIP
T7345 16385-16386 . denotes .
T7346 16387-16389 TO denotes To
T7347 16390-16399 VB denotes determine
T7348 16400-16404 WP denotes what
T7349 16405-16408 VBD denotes was
T7350 16409-16420 JJ denotes responsible
T7351 16421-16424 IN denotes for
T7352 16425-16428 DT denotes the
T7353 16429-16438 VBN denotes increased
T7354 16439-16448 JJ denotes molecular
T7355 16449-16455 NN denotes weight
T7356 16456-16458 IN denotes of
T7357 16459-16463 NNP denotes IKKγ
T7358 16464-16466 IN denotes in
T7359 16467-16471 NNP denotes CD40
T7360 16472-16490 NNS denotes immunoprecipitates
T7361 16490-16491 , denotes ,
T7362 16492-16495 DT denotes the
T7363 16496-16503 NN denotes protein
T7364 16504-16511 NNS denotes samples
T7365 16512-16516 VBD denotes were
T7366 16517-16526 VBN denotes incubated
T7367 16527-16531 IN denotes with
T7368 16532-16538 JJ denotes lambda
T7369 16539-16550 NN denotes phosphatase
T7370 16550-16551 , denotes ,
T7371 16552-16557 WDT denotes which
T7372 16558-16565 VBZ denotes removes
T7373 16566-16576 NNS denotes phosphates
T7374 16577-16585 VBN denotes attached
T7375 16586-16588 TO denotes to
T7376 16589-16597 VB denotes tyrosine
T7377 16597-16598 , denotes ,
T7378 16599-16608 NN denotes threonine
T7379 16608-16609 , denotes ,
T7380 16610-16612 CC denotes or
T7381 16613-16619 NN denotes serine
T7382 16620-16628 NNS denotes residues
T7383 16629-16631 IN denotes in
T7384 16632-16640 NNS denotes proteins
T7385 16640-16641 . denotes .
T7386 16642-16646 DT denotes This
T7387 16647-16656 NN denotes treatment
T7388 16657-16664 VBN denotes reduced
T7389 16665-16669 RB denotes much
T7390 16670-16672 IN denotes of
T7391 16673-16688 JJ denotes CD40-associated
T7392 16689-16693 NNP denotes IKKγ
T7393 16694-16696 TO denotes to
T7394 16697-16699 DT denotes an
T7395 16700-16708 JJ denotes apparent
T7396 16709-16718 JJ denotes molecular
T7397 16719-16725 NN denotes weight
T7398 16726-16733 JJ denotes similar
T7399 16734-16736 TO denotes to
T7400 16737-16741 DT denotes that
T7401 16742-16744 IN denotes of
T7402 16745-16748 DT denotes the
T7403 16749-16756 NN denotes protein
T7404 16757-16759 IN denotes in
T7405 16760-16764 NN denotes cell
T7406 16765-16772 NNS denotes lysates
T7407 16773-16774 -LRB- denotes (
T7408 16774-16778 NNP denotes Fig.
T7409 16779-16781 NNP denotes 5C
T7410 16781-16782 -RRB- denotes )
T7411 16782-16783 . denotes .
T7412 16784-16791 RB denotes However
T7413 16791-16792 , denotes ,
T7414 16793-16795 IN denotes at
T7415 16796-16801 JJS denotes least
T7416 16802-16805 CD denotes one
T7417 16806-16810 NN denotes band
T7418 16811-16813 IN denotes of
T7419 16814-16827 RB denotes significantly
T7420 16828-16834 JJR denotes higher
T7421 16835-16844 JJ denotes molecular
T7422 16845-16851 NN denotes weight
T7423 16852-16860 VBD denotes remained
T7424 16861-16866 IN denotes after
T7425 16867-16878 NN denotes phosphatase
T7426 16879-16888 NN denotes treatment
T7427 16888-16889 , denotes ,
T7428 16890-16900 VBG denotes suggesting
T7429 16901-16905 IN denotes that
T7430 16906-16921 JJ denotes CD40-associated
T7431 16922-16926 NNP denotes IKKγ
T7432 16927-16929 VBZ denotes is
T7433 16930-16937 JJ denotes subject
T7434 16938-16940 TO denotes to
T7435 16941-16943 IN denotes at
T7436 16944-16949 JJS denotes least
T7437 16950-16953 CD denotes one
T7438 16954-16959 JJ denotes other
T7439 16960-16972 NN denotes modification
T7440 16973-16975 IN denotes in
T7441 16976-16984 NN denotes addition
T7442 16985-16987 TO denotes to
T7443 16988-17003 NN denotes phosphorylation
T7444 17003-17004 . denotes .
T7445 17005-17012 RB denotes Overall
T7446 17012-17013 , denotes ,
T7447 17014-17019 DT denotes these
T7448 17020-17024 NNS denotes data
T7449 17025-17036 VBP denotes demonstrate
T7450 17037-17041 IN denotes that
T7451 17042-17046 NNP denotes HOIP
T7452 17047-17049 VBZ denotes is
T7453 17050-17058 VBN denotes required
T7454 17059-17062 IN denotes for
T7455 17063-17066 DT denotes the
T7456 17067-17078 NN denotes association
T7457 17079-17081 IN denotes of
T7458 17082-17086 NNP denotes IKKγ
T7459 17087-17091 IN denotes with
T7460 17092-17096 NNP denotes CD40
T7461 17096-17097 , denotes ,
T7462 17098-17101 CC denotes and
T7463 17102-17109 VBP denotes suggest
T7464 17110-17114 IN denotes that
T7465 17115-17119 DT denotes this
T7466 17120-17125 NN denotes event
T7467 17126-17128 VBZ denotes is
T7468 17129-17136 VBN denotes coupled
T7469 17137-17139 TO denotes to
T7470 17140-17158 JJ denotes post-translational
T7471 17159-17172 NNS denotes modifications
T7472 17173-17175 IN denotes of
T7473 17176-17180 NNP denotes IKKγ
T7474 17180-17181 . denotes .
T14807 2125-2129 NNP denotes HOIP
T14808 2130-2134 NN denotes gene
T14809 2135-2144 VBG denotes targeting
T14810 2144-2145 . denotes .
T14811 2146-2147 -LRB- denotes (
T14812 2147-2148 NNP denotes A
T14813 2148-2149 -RRB- denotes )
T14814 2150-2157 NNS denotes Regions
T14815 2158-2160 IN denotes of
T14816 2161-2166 JJ denotes Rnf31
T14817 2167-2171 NN denotes gene
T14818 2172-2180 NN denotes sequence
T14819 2181-2182 -LRB- denotes (
T14820 2182-2188 NN denotes bottom
T14821 2188-2189 -RRB- denotes )
T14822 2190-2194 VBN denotes used
T14823 2195-2197 IN denotes in
T14824 2198-2201 DT denotes the
T14825 2202-2206 NNP denotes HOIP
T14826 2207-2211 NN denotes gene
T14827 2212-2221 VBG denotes targeting
T14828 2222-2228 NN denotes vector
T14829 2229-2230 -LRB- denotes (
T14830 2230-2233 JJ denotes top
T14831 2233-2234 -RRB- denotes )
T14832 2235-2238 VBP denotes are
T14833 2239-2244 VBN denotes shown
T14834 2244-2245 . denotes .
T14835 2246-2252 NNS denotes Arrows
T14836 2253-2254 -LRB- denotes (
T14837 2254-2255 NN denotes F
T14838 2256-2259 CC denotes and
T14839 2260-2261 NN denotes R
T14840 2261-2262 -RRB- denotes )
T14841 2263-2271 VBP denotes indicate
T14842 2272-2283 JJ denotes approximate
T14843 2284-2293 NNS denotes positions
T14844 2294-2296 IN denotes of
T14845 2297-2306 NNS denotes sequences
T14846 2307-2317 RB denotes homologous
T14847 2318-2320 TO denotes to
T14848 2321-2337 NNS denotes oligonucleotides
T14849 2338-2342 VBN denotes used
T14850 2343-2346 IN denotes for
T14851 2347-2359 JJ denotes PCR-mediated
T14852 2360-2369 NN denotes detection
T14853 2370-2372 IN denotes of
T14854 2373-2383 RB denotes homologous
T14855 2384-2397 NN denotes recombination
T14856 2397-2398 . denotes .
T14857 2399-2409 JJ denotes Homologous
T14858 2410-2423 NN denotes recombination
T14859 2424-2426 IN denotes of
T14860 2427-2430 DT denotes the
T14861 2431-2437 NN denotes vector
T14862 2438-2442 IN denotes with
T14863 2443-2448 JJ denotes Rnf31
T14864 2449-2457 VBD denotes resulted
T14865 2458-2460 IN denotes in
T14866 2461-2462 DT denotes a
T14867 2463-2468 JJ denotes small
T14868 2469-2480 JJ denotes chromosomal
T14869 2481-2489 NN denotes deletion
T14870 2490-2493 CC denotes and
T14871 2494-2497 DT denotes the
T14872 2498-2506 JJ denotes in-frame
T14873 2507-2516 NN denotes insertion
T14874 2517-2519 IN denotes of
T14875 2520-2528 NN denotes neomycin
T14876 2529-2547 NN denotes phosphotransferase
T14877 2548-2549 -LRB- denotes (
T14878 2549-2553 NNP denotes NeoR
T14879 2553-2554 -RRB- denotes )
T14880 2555-2559 IN denotes into
T14881 2560-2563 DT denotes the
T14882 2564-2578 JJ denotes amino-terminal
T14883 2579-2583 NNP denotes HOIP
T14884 2584-2590 NN denotes coding
T14885 2591-2599 NN denotes sequence
T14886 2599-2600 . denotes .
T14887 2601-2602 DT denotes A
T14888 2603-2613 NN denotes diphtheria
T14889 2614-2619 NN denotes toxin
T14890 2620-2621 -LRB- denotes (
T14891 2621-2623 NNP denotes DT
T14892 2623-2624 -RRB- denotes )
T14893 2625-2633 NN denotes cassette
T14894 2634-2636 IN denotes in
T14895 2637-2640 DT denotes the
T14896 2641-2647 NN denotes vector
T14897 2648-2659 VBZ denotes facilitates
T14898 2660-2663 DT denotes the
T14899 2664-2672 JJ denotes negative
T14900 2673-2682 NN denotes selection
T14901 2683-2685 IN denotes of
T14902 2686-2691 NNS denotes cells
T14903 2692-2694 IN denotes in
T14904 2695-2700 WDT denotes which
T14905 2701-2707 JJ denotes random
T14906 2708-2719 JJ denotes chromosomal
T14907 2720-2729 NN denotes insertion
T14908 2730-2732 IN denotes of
T14909 2733-2736 DT denotes the
T14910 2737-2743 NN denotes vector
T14911 2744-2749 VBZ denotes takes
T14912 2750-2755 NN denotes place
T14913 2755-2756 . denotes .
T14914 2757-2761 NNP denotes LoxP
T14915 2762-2771 NNS denotes sequences
T14916 2772-2777 VBP denotes allow
T14917 2778-2781 DT denotes the
T14918 2782-2794 JJ denotes Cre-mediated
T14919 2795-2803 NN denotes deletion
T14920 2804-2806 IN denotes of
T14921 2807-2810 DT denotes the
T14922 2811-2815 NNP denotes NeoR
T14923 2816-2822 NN denotes coding
T14924 2823-2831 NN denotes sequence
T14925 2831-2832 . denotes .
T14926 2833-2836 DT denotes The
T14927 2837-2843 NNP denotes SV40pA
T14928 2844-2852 NN denotes sequence
T14929 2853-2858 VBZ denotes helps
T14930 2859-2861 TO denotes to
T14931 2862-2868 VB denotes ensure
T14932 2869-2879 NN denotes disruption
T14933 2880-2882 IN denotes of
T14934 2883-2887 NN denotes gene
T14935 2888-2898 NN denotes expression
T14936 2899-2904 IN denotes after
T14937 2905-2913 NN denotes deletion
T14938 2914-2916 IN denotes of
T14939 2917-2920 DT denotes the
T14940 2921-2925 NNP denotes NeoR
T14941 2926-2934 NN denotes sequence
T14942 2934-2935 . denotes .
T14943 2936-2937 -LRB- denotes (
T14944 2937-2938 NNP denotes B
T14945 2938-2939 -RRB- denotes )
T14946 2940-2949 NNP denotes Anti-HOIP
T14947 2950-2953 CC denotes and
T14948 2954-2963 NNP denotes anti-FLAG
T14949 2964-2971 NNP denotes Western
T14950 2972-2977 NNS denotes blots
T14951 2978-2980 IN denotes of
T14952 2981-2985 NN denotes cell
T14953 2986-2993 NNS denotes lysates
T14954 2994-2998 IN denotes from
T14955 2999-3002 NNP denotes A20
T14956 3002-3004 CD denotes .2
T14957 3004-3005 NNP denotes J
T14958 3006-3011 NNS denotes cells
T14959 3012-3015 CC denotes and
T14960 3016-3030 JJ denotes HOIP-deficient
T14961 3031-3032 -LRB- denotes (
T14962 3032-3036 SYM denotes HOIP
T14963 3036-3037 : denotes -
T14964 3037-3038 SYM denotes /
T14965 3038-3039 : denotes -
T14966 3039-3040 -RRB- denotes )
T14967 3041-3047 NNS denotes clones
T14968 3047-3048 . denotes .
T14969 3049-3050 DT denotes A
T14970 3051-3058 JJ denotes partial
T14971 3059-3067 NN denotes decrease
T14972 3068-3070 IN denotes in
T14973 3071-3075 NNP denotes HOIP
T14974 3076-3083 NN denotes protein
T14975 3084-3094 NN denotes expression
T14976 3095-3097 VBZ denotes is
T14977 3098-3105 JJ denotes evident
T14978 3106-3108 IN denotes in
T14979 3109-3114 NNS denotes cells
T14980 3115-3124 VBG denotes following
T14981 3125-3135 NN denotes disruption
T14982 3136-3138 IN denotes of
T14983 3139-3142 CD denotes one
T14984 3143-3147 NN denotes copy
T14985 3148-3150 IN denotes of
T14986 3151-3156 NNP denotes Rnf31
T14987 3157-3158 -LRB- denotes (
T14988 3158-3162 NNP denotes HOIP
T14989 3162-3163 CD denotes −
T14990 3163-3164 CD denotes /
T14991 3164-3165 NN denotes +
T14992 3165-3166 -RRB- denotes )
T14993 3166-3167 . denotes .
T14994 3168-3172 NNP denotes HOIP
T14995 3173-3183 NN denotes expression
T14996 3184-3186 IN denotes in
T14997 3187-3193 NNS denotes clones
T14998 3194-3207 VBN denotes reconstituted
T14999 3208-3212 IN denotes with
T15000 3213-3215 DT denotes an
T15001 3216-3221 JJ denotes empty
T15002 3222-3232 NN denotes retroviral
T15003 3233-3239 NN denotes vector
T15004 3240-3241 -LRB- denotes (
T15005 3241-3245 NNP denotes pMIP
T15006 3245-3246 -RRB- denotes )
T15007 3247-3249 CC denotes or
T15008 3250-3251 DT denotes a
T15009 3252-3262 JJ denotes retroviral
T15010 3263-3269 NN denotes vector
T15011 3270-3278 VBG denotes encoding
T15012 3279-3290 JJ denotes FLAG-tagged
T15013 3291-3295 NNP denotes HOIP
T15014 3296-3298 VBZ denotes is
T15015 3299-3303 RB denotes also
T15016 3304-3309 VBN denotes shown
T15017 3309-3310 . denotes .
T15018 3311-3322 JJ denotes Approximate
T15019 3323-3332 JJ denotes molecular
T15020 3333-3339 NN denotes weight
T15021 3340-3342 IN denotes of
T15022 3343-3347 NNP denotes HOIP
T15023 3348-3350 VBZ denotes is
T15024 3351-3354 CD denotes 120
T15025 3355-3357 NN denotes kD
T15026 3357-3358 . denotes .
T15392 4763-4776 JJ denotes CD40-mediated
T15393 4777-4781 NN denotes CD80
T15394 4782-4794 NN denotes upregulation
T15395 4795-4797 VBZ denotes is
T15396 4798-4807 JJ denotes defective
T15397 4808-4810 IN denotes in
T15398 4811-4825 JJ denotes HOIP-deficient
T15399 4826-4831 NNS denotes cells
T15400 4831-4832 . denotes .
T15401 4833-4838 NNS denotes Cells
T15402 4839-4843 VBD denotes were
T15403 4844-4853 VBN denotes incubated
T15404 4854-4857 IN denotes for
T15405 4858-4860 CD denotes 72
T15406 4861-4864 NNS denotes hrs
T15407 4865-4869 IN denotes with
T15408 4870-4872 DT denotes an
T15409 4873-4880 NN denotes isotype
T15410 4881-4888 NN denotes control
T15411 4889-4897 NN denotes antibody
T15412 4898-4899 -LRB- denotes (
T15413 4899-4903 VBN denotes left
T15414 4903-4904 -RRB- denotes )
T15415 4905-4907 CC denotes or
T15416 4908-4917 JJ denotes anti-CD40
T15417 4918-4926 NN denotes antibody
T15418 4927-4928 -LRB- denotes (
T15419 4928-4933 NN denotes right
T15420 4933-4934 -RRB- denotes )
T15421 4935-4938 CC denotes and
T15422 4939-4943 RB denotes then
T15423 4944-4951 VBD denotes stained
T15424 4952-4955 IN denotes for
T15425 4956-4966 NN denotes expression
T15426 4967-4969 IN denotes of
T15427 4970-4974 NNP denotes CD80
T15428 4975-4976 -LRB- denotes (
T15429 4976-4982 VBN denotes filled
T15430 4983-4991 NNS denotes profiles
T15431 4992-5000 VBP denotes indicate
T15432 5001-5009 VBG denotes staining
T15433 5010-5014 IN denotes with
T15434 5015-5024 JJ denotes anti-CD80
T15435 5025-5033 NN denotes antibody
T15436 5033-5034 : denotes ;
T15437 5035-5039 JJ denotes open
T15438 5040-5048 NNS denotes profiles
T15439 5049-5052 VBP denotes are
T15440 5053-5061 VBG denotes staining
T15441 5062-5066 IN denotes with
T15442 5067-5069 DT denotes an
T15443 5070-5077 NN denotes isotype
T15444 5078-5085 NN denotes control
T15445 5086-5094 NN denotes antibody
T15446 5094-5095 -RRB- denotes )
T15447 5095-5096 . denotes .
T15448 5097-5109 NNP denotes Transduction
T15449 5110-5112 IN denotes of
T15450 5113-5117 DT denotes both
T15451 5118-5132 JJ denotes HOIP-deficient
T15452 5133-5134 -LRB- denotes (
T15453 5134-5138 SYM denotes HOIP
T15454 5138-5139 : denotes -
T15455 5139-5140 SYM denotes /
T15456 5140-5141 : denotes -
T15457 5141-5142 -RRB- denotes )
T15458 5143-5149 NNS denotes clones
T15459 5150-5154 IN denotes with
T15460 5155-5156 DT denotes a
T15461 5157-5167 NN denotes retrovirus
T15462 5168-5176 VBG denotes encoding
T15463 5177-5186 NNP denotes FLAG-HOIP
T15464 5187-5195 VBD denotes reversed
T15465 5196-5205 JJ denotes defective
T15466 5206-5210 NNP denotes CD80
T15467 5211-5223 NN denotes upregulation
T15468 5223-5224 , denotes ,
T15469 5225-5230 IN denotes while
T15470 5231-5243 NN denotes transduction
T15471 5244-5248 IN denotes with
T15472 5249-5251 DT denotes an
T15473 5252-5257 JJ denotes empty
T15474 5258-5264 NN denotes vector
T15475 5265-5266 -LRB- denotes (
T15476 5266-5270 NNP denotes pMIP
T15477 5270-5271 -RRB- denotes )
T15478 5272-5275 VBD denotes did
T15479 5276-5279 RB denotes not
T15480 5279-5280 . denotes .
T15481 5281-5288 JJ denotes Similar
T15482 5289-5296 NNS denotes results
T15483 5297-5301 VBD denotes were
T15484 5302-5310 VBN denotes obtained
T15485 5311-5313 IN denotes in
T15486 5314-5315 DT denotes a
T15487 5316-5322 JJ denotes second
T15488 5323-5333 NN denotes experiment
T15489 5333-5334 . denotes .
T15742 6765-6768 NNP denotes GLε
T15743 6769-6782 NN denotes transcription
T15744 6783-6785 VBZ denotes is
T15745 6786-6795 JJ denotes defective
T15746 6796-6798 IN denotes in
T15747 6799-6814 JJ denotes CD40-stimulated
T15748 6815-6829 JJ denotes HOIP-deficient
T15749 6830-6835 NNS denotes cells
T15750 6835-6836 . denotes .
T15751 6837-6840 CD denotes A20
T15752 6840-6842 CD denotes .2
T15753 6842-6843 NNP denotes J
T15754 6844-6847 CC denotes and
T15755 6848-6862 JJ denotes HOIP-deficient
T15756 6863-6868 NNS denotes cells
T15757 6869-6879 VBN denotes transduced
T15758 6880-6884 IN denotes with
T15759 6885-6887 DT denotes an
T15760 6888-6893 JJ denotes empty
T15761 6894-6904 NN denotes retroviral
T15762 6905-6911 NN denotes vector
T15763 6912-6913 -LRB- denotes (
T15764 6913-6916 CD denotes A20
T15765 6916-6918 CD denotes .2
T15766 6918-6919 NNP denotes J
T15767 6920-6921 CD denotes +
T15768 6922-6926 NNP denotes pMIP
T15769 6927-6930 CC denotes and
T15770 6931-6935 NNP denotes HOIP
T15771 6935-6936 : denotes -
T15772 6936-6937 SYM denotes /
T15773 6937-6938 : denotes -
T15774 6939-6940 NN denotes +
T15775 6941-6945 NNP denotes pMIP
T15776 6945-6946 , denotes ,
T15777 6947-6959 RB denotes respectively
T15778 6959-6960 -RRB- denotes )
T15779 6960-6961 , denotes ,
T15780 6962-6964 CC denotes or
T15781 6965-6979 JJ denotes HOIP-deficient
T15782 6980-6985 NNS denotes cells
T15783 6986-6996 VBN denotes transduced
T15784 6997-7001 IN denotes with
T15785 7002-7003 DT denotes a
T15786 7004-7017 JJ denotes HOIP-encoding
T15787 7018-7028 NN denotes retrovirus
T15788 7029-7030 -LRB- denotes (
T15789 7030-7034 SYM denotes HOIP
T15790 7034-7035 : denotes -
T15791 7035-7036 SYM denotes /
T15792 7036-7037 : denotes -
T15793 7038-7039 NN denotes +
T15794 7040-7044 NNP denotes HOIP
T15795 7044-7045 -RRB- denotes )
T15796 7046-7050 VBD denotes were
T15797 7051-7059 VBN denotes cultured
T15798 7060-7069 JJ denotes overnight
T15799 7070-7074 IN denotes with
T15800 7075-7084 JJ denotes agonistic
T15801 7085-7094 JJ denotes anti-CD40
T15802 7095-7097 CC denotes or
T15803 7098-7100 DT denotes an
T15804 7101-7108 NN denotes isotype
T15805 7109-7116 NN denotes control
T15806 7117-7125 NN denotes antibody
T15807 7126-7127 -LRB- denotes (
T15808 7127-7130 FW denotes iso
T15809 7130-7131 -RRB- denotes )
T15810 7131-7132 , denotes ,
T15811 7133-7137 IN denotes with
T15812 7138-7140 CC denotes or
T15813 7141-7148 IN denotes without
T15814 7149-7153 NN denotes IL-4
T15815 7153-7154 . denotes .
T15816 7155-7158 NNP denotes RNA
T15817 7159-7162 VBD denotes was
T15818 7163-7171 VBN denotes isolated
T15819 7172-7176 IN denotes from
T15820 7177-7180 DT denotes the
T15821 7181-7186 NNS denotes cells
T15822 7186-7187 , denotes ,
T15823 7188-7207 JJ denotes reverse-transcribed
T15824 7207-7208 , denotes ,
T15825 7209-7212 CC denotes and
T15826 7213-7217 RB denotes then
T15827 7218-7227 VBN denotes subjected
T15828 7228-7230 TO denotes to
T15829 7231-7243 JJ denotes quantitative
T15830 7244-7247 NNP denotes PCR
T15831 7248-7250 TO denotes to
T15832 7251-7260 VB denotes determine
T15833 7261-7267 NNS denotes levels
T15834 7268-7270 IN denotes of
T15835 7271-7274 NNP denotes GLε
T15836 7275-7286 NNS denotes transcripts
T15837 7286-7287 . denotes .
T15838 7288-7295 NNS denotes Results
T15839 7296-7300 VBD denotes were
T15840 7301-7311 VBN denotes normalized
T15841 7312-7314 TO denotes to
T15842 7315-7318 DT denotes the
T15843 7319-7325 NNS denotes levels
T15844 7326-7328 IN denotes of
T15845 7329-7334 JJ denotes Hprt1
T15846 7335-7346 NNS denotes transcripts
T15847 7347-7349 IN denotes in
T15848 7350-7354 DT denotes each
T15849 7355-7361 NN denotes sample
T15850 7361-7362 . denotes .
T15851 7363-7370 NNS denotes Symbols
T15852 7371-7379 VBP denotes indicate
T15853 7380-7383 DT denotes the
T15854 7384-7390 NNS denotes values
T15855 7391-7395 IN denotes from
T15856 7396-7405 VBP denotes duplicate
T15857 7406-7414 NNS denotes cultures
T15858 7415-7416 -LRB- denotes (
T15859 7416-7417 DT denotes a
T15860 7418-7422 NN denotes line
T15861 7423-7432 VBZ denotes indicates
T15862 7433-7436 DT denotes the
T15863 7437-7441 NN denotes mean
T15864 7442-7444 IN denotes of
T15865 7445-7448 DT denotes the
T15866 7449-7452 CD denotes two
T15867 7453-7459 NNS denotes values
T15868 7459-7460 -RRB- denotes )
T15869 7460-7461 . denotes .
T15870 7462-7469 JJ denotes Similar
T15871 7470-7477 NNS denotes results
T15872 7478-7482 VBD denotes were
T15873 7483-7491 VBN denotes obtained
T15874 7492-7494 IN denotes in
T15875 7495-7496 DT denotes a
T15876 7497-7503 JJ denotes second
T15877 7504-7514 NN denotes experiment
T15878 7515-7518 CC denotes and
T15879 7519-7521 IN denotes in
T15880 7522-7524 DT denotes an
T15881 7525-7535 JJ denotes additional
T15882 7536-7546 NN denotes experiment
T15883 7547-7551 IN denotes with
T15884 7552-7553 DT denotes a
T15885 7554-7560 JJ denotes second
T15886 7561-7575 JJ denotes HOIP-deficient
T15887 7576-7581 NN denotes clone
T15888 7581-7582 . denotes .
T16192 9287-9299 JJ denotes CD40-induced
T16193 9300-9310 NN denotes activation
T16194 9311-9313 IN denotes of
T16195 9314-9319 NNP denotes NF-κB
T16196 9320-9323 CC denotes and
T16197 9324-9327 NNP denotes JNK
T16198 9328-9330 VBZ denotes is
T16199 9331-9340 JJ denotes defective
T16200 9341-9343 IN denotes in
T16201 9344-9358 JJ denotes HOIP-deficient
T16202 9359-9364 NNS denotes cells
T16203 9364-9365 . denotes .
T16204 9366-9379 JJ denotes CD40-mediated
T16205 9380-9389 VBG denotes signaling
T16206 9390-9393 VBD denotes was
T16207 9394-9403 JJ denotes defective
T16208 9404-9406 IN denotes in
T16209 9407-9421 JJ denotes HOIP-deficient
T16210 9422-9427 NNS denotes cells
T16211 9428-9429 -LRB- denotes (
T16212 9429-9433 SYM denotes HOIP
T16213 9433-9434 : denotes -
T16214 9434-9435 SYM denotes /
T16215 9435-9436 : denotes -
T16216 9437-9438 NN denotes +
T16217 9439-9443 NNP denotes pMIP
T16218 9443-9444 -RRB- denotes )
T16219 9444-9445 , denotes ,
T16220 9446-9449 CC denotes but
T16221 9450-9456 JJ denotes intact
T16222 9457-9459 IN denotes in
T16223 9460-9468 JJ denotes parental
T16224 9469-9474 NNS denotes cells
T16225 9475-9485 VBN denotes transduced
T16226 9486-9490 IN denotes with
T16227 9491-9493 DT denotes an
T16228 9494-9499 JJ denotes empty
T16229 9500-9510 NN denotes retroviral
T16230 9511-9517 NN denotes vector
T16231 9518-9519 -LRB- denotes (
T16232 9519-9522 CD denotes A20
T16233 9522-9524 CD denotes .2
T16234 9524-9525 NNP denotes J
T16235 9526-9527 CD denotes +
T16236 9528-9532 NNP denotes pMIP
T16237 9532-9533 -RRB- denotes )
T16238 9534-9537 CC denotes and
T16239 9538-9540 IN denotes in
T16240 9541-9555 JJ denotes HOIP-deficient
T16241 9556-9561 NNS denotes cells
T16242 9562-9572 VBN denotes transduced
T16243 9573-9577 IN denotes with
T16244 9578-9579 DT denotes a
T16245 9580-9590 JJ denotes retroviral
T16246 9591-9597 NN denotes vector
T16247 9598-9606 VBG denotes encoding
T16248 9607-9611 NNP denotes HOIP
T16249 9612-9613 -LRB- denotes (
T16250 9613-9617 NNP denotes HOIP
T16251 9617-9618 : denotes -
T16252 9618-9619 SYM denotes /
T16253 9619-9620 : denotes -
T16254 9621-9622 NN denotes +
T16255 9623-9627 NNP denotes HOIP
T16256 9627-9628 -RRB- denotes )
T16257 9628-9629 . denotes .
T16258 9630-9635 NNS denotes Cells
T16259 9636-9640 VBD denotes were
T16260 9641-9650 VBN denotes activated
T16261 9651-9655 IN denotes with
T16262 9656-9672 JJ denotes CD154-expressing
T16263 9673-9679 NN denotes insect
T16264 9680-9685 NNS denotes cells
T16265 9686-9689 IN denotes for
T16266 9690-9693 DT denotes the
T16267 9694-9699 NNS denotes times
T16268 9700-9709 VBD denotes indicated
T16269 9709-9710 . denotes .
T16270 9711-9713 IN denotes As
T16271 9714-9715 DT denotes a
T16272 9716-9724 JJ denotes negative
T16273 9725-9732 NN denotes control
T16274 9732-9733 , denotes ,
T16275 9734-9739 NNS denotes cells
T16276 9740-9744 VBD denotes were
T16277 9745-9755 VBN denotes stimulated
T16278 9756-9760 IN denotes with
T16279 9761-9767 JJ denotes insect
T16280 9768-9773 NNS denotes cells
T16281 9774-9781 VBG denotes lacking
T16282 9782-9787 NNP denotes CD154
T16283 9788-9789 -LRB- denotes (
T16284 9789-9790 CD denotes 5
T16285 9791-9797 JJ denotes minute
T16286 9798-9802 NN denotes time
T16287 9803-9808 NN denotes point
T16288 9809-9813 RB denotes only
T16289 9813-9814 -RRB- denotes )
T16290 9814-9815 . denotes .
T16291 9816-9823 JJ denotes Western
T16292 9824-9829 NNS denotes blots
T16293 9830-9832 IN denotes of
T16294 9833-9843 JJ denotes whole-cell
T16295 9844-9851 NNS denotes lysates
T16296 9852-9856 VBD denotes were
T16297 9857-9863 VBN denotes probed
T16298 9864-9868 IN denotes with
T16299 9869-9872 DT denotes the
T16300 9873-9882 VBN denotes indicated
T16301 9883-9893 NNS denotes antibodies
T16302 9893-9894 . denotes .
T16303 9895-9907 NNP denotes Phospho-IκBα
T16304 9908-9909 -LRB- denotes (
T16305 9909-9914 NNP denotes pIκBα
T16306 9914-9915 -RRB- denotes )
T16307 9916-9919 CC denotes and
T16308 9920-9931 NNP denotes phospho-JNK
T16309 9932-9933 -LRB- denotes (
T16310 9933-9937 NNP denotes pJNK
T16311 9937-9938 -RRB- denotes )
T16312 9939-9944 NNS denotes blots
T16313 9945-9949 VBD denotes were
T16314 9950-9958 VBN denotes stripped
T16315 9959-9962 CC denotes and
T16316 9963-9971 VBN denotes reprobed
T16317 9972-9975 IN denotes for
T16318 9976-9981 JJ denotes total
T16319 9982-9986 NNP denotes IκBα
T16320 9987-9990 CC denotes and
T16321 9991-9996 JJ denotes total
T16322 9997-10000 NNP denotes JNK
T16323 10000-10001 , denotes ,
T16324 10002-10014 RB denotes respectively
T16325 10015-10016 -LRB- denotes (
T16326 10016-10024 JJ denotes anti-JNK
T16327 10025-10035 NNS denotes antibodies
T16328 10036-10045 VBP denotes recognize
T16329 10046-10049 CD denotes p46
T16330 10050-10053 CC denotes and
T16331 10054-10057 CD denotes p54
T16332 10058-10066 NNS denotes isoforms
T16333 10066-10067 -RRB- denotes )
T16334 10067-10068 . denotes .
T16335 10069-10073 NNP denotes IκBα
T16336 10074-10079 NNS denotes blots
T16337 10080-10084 VBD denotes were
T16338 10085-10089 RB denotes also
T16339 10090-10098 VBN denotes reprobed
T16340 10099-10102 IN denotes for
T16341 10103-10108 NN denotes actin
T16342 10109-10111 TO denotes to
T16343 10112-10123 VB denotes demonstrate
T16344 10124-10129 JJ denotes equal
T16345 10130-10134 NN denotes lane
T16346 10135-10142 VBG denotes loading
T16347 10142-10143 . denotes .
T16348 10144-10153 JJ denotes Molecular
T16349 10154-10161 NNS denotes weights
T16350 10162-10165 VBP denotes are
T16351 10166-10175 VBN denotes indicated
T16352 10176-10178 IN denotes at
T16353 10179-10184 NN denotes right
T16354 10184-10185 . denotes .
T16355 10186-10193 JJ denotes Similar
T16356 10194-10201 NNS denotes results
T16357 10202-10206 VBD denotes were
T16358 10207-10215 VBN denotes obtained
T16359 10216-10218 IN denotes in
T16360 10219-10220 DT denotes a
T16361 10221-10227 JJ denotes second
T16362 10228-10238 NN denotes experiment
T16363 10239-10242 CC denotes and
T16364 10243-10245 IN denotes in
T16365 10246-10249 CD denotes two
T16366 10250-10260 JJ denotes additional
T16367 10261-10272 NNS denotes experiments
T16368 10273-10277 IN denotes with
T16369 10278-10279 DT denotes a
T16370 10280-10286 JJ denotes second
T16371 10287-10301 JJ denotes HOIP-deficient
T16372 10302-10307 NN denotes clone
T16373 10307-10308 . denotes .
T16830 12073-12076 NNP denotes IKK
T16831 12077-12088 NN denotes recruitment
T16832 12089-12091 TO denotes to
T16833 12092-12095 DT denotes the
T16834 12096-12100 NNP denotes CD40
T16835 12101-12110 VBG denotes signaling
T16836 12111-12118 NN denotes complex
T16837 12119-12121 VBZ denotes is
T16838 12122-12131 JJ denotes defective
T16839 12132-12134 IN denotes in
T16840 12135-12149 JJ denotes HOIP-deficient
T16841 12150-12155 NNS denotes cells
T16842 12155-12156 . denotes .
T16843 12157-12158 -LRB- denotes (
T16844 12158-12159 NNP denotes A
T16845 12159-12160 -RRB- denotes )
T16846 12161-12165 NNP denotes SMAC
T16847 12166-12173 NN denotes peptide
T16848 12174-12183 NN denotes treatment
T16849 12184-12191 VBZ denotes reduces
T16850 12192-12203 NN denotes recruitment
T16851 12204-12206 IN denotes of
T16852 12207-12212 NNP denotes cIAP1
T16853 12213-12215 TO denotes to
T16854 12216-12220 NNP denotes CD40
T16855 12221-12224 CC denotes and
T16856 12225-12228 MD denotes may
T16857 12229-12235 VB denotes modify
T16858 12236-12240 NNP denotes HOIP
T16859 12241-12252 NN denotes recruitment
T16860 12252-12253 . denotes .
T16861 12254-12257 CD denotes A20
T16862 12257-12259 CD denotes .2
T16863 12259-12260 NNP denotes J
T16864 12261-12266 NNS denotes cells
T16865 12267-12271 VBD denotes were
T16866 12272-12281 VBN denotes incubated
T16867 12282-12285 IN denotes for
T16868 12286-12289 CD denotes six
T16869 12290-12295 NNS denotes hours
T16870 12296-12300 IN denotes with
T16871 12301-12319 JJ denotes membrane-permeable
T16872 12320-12327 NNP denotes SMAC-N7
T16873 12328-12335 NN denotes peptide
T16874 12336-12338 CC denotes or
T16875 12339-12342 CD denotes 1.5
T16876 12342-12343 NN denotes %
T16877 12344-12348 NNP denotes DMSO
T16878 12349-12350 -LRB- denotes (
T16879 12350-12357 JJ denotes solvent
T16880 12358-12362 VBN denotes used
T16881 12363-12366 IN denotes for
T16882 12367-12370 DT denotes the
T16883 12371-12378 NN denotes peptide
T16884 12378-12379 -RRB- denotes )
T16885 12379-12380 . denotes .
T16886 12381-12390 VBG denotes Following
T16887 12391-12394 DT denotes the
T16888 12395-12405 NN denotes incubation
T16889 12405-12406 , denotes ,
T16890 12407-12411 NN denotes cell
T16891 12412-12419 NNS denotes lysates
T16892 12420-12424 VBD denotes were
T16893 12425-12433 VBN denotes prepared
T16894 12433-12434 , denotes ,
T16895 12435-12447 VBN denotes fractionated
T16896 12448-12450 IN denotes by
T16897 12451-12459 NNP denotes SDS-PAGE
T16898 12459-12460 , denotes ,
T16899 12461-12464 CC denotes and
T16900 12465-12474 VBN denotes evaluated
T16901 12475-12477 IN denotes by
T16902 12478-12485 JJ denotes Western
T16903 12486-12490 NN denotes blot
T16904 12491-12492 -LRB- denotes (
T16905 12492-12497 NNS denotes lanes
T16906 12498-12499 CD denotes 1
T16907 12500-12503 CC denotes and
T16908 12504-12505 CD denotes 2
T16909 12505-12506 -RRB- denotes )
T16910 12506-12507 . denotes .
T16911 12508-12513 NNS denotes Cells
T16912 12514-12523 VBD denotes incubated
T16913 12524-12528 IN denotes with
T16914 12529-12533 NNP denotes DMSO
T16915 12534-12536 CC denotes or
T16916 12537-12544 NNP denotes SMAC-N7
T16918 12550-12554 RB denotes also
T16919 12555-12565 VBN denotes stimulated
T16920 12566-12570 IN denotes with
T16921 12571-12579 JJ denotes magnetic
T16922 12580-12585 NNS denotes beads
T16923 12586-12592 VBN denotes coated
T16924 12593-12597 IN denotes with
T16925 12598-12607 JJ denotes anti-CD40
T16926 13100-13104 IN denotes with
T16927 12608-12610 CC denotes or
T16928 12611-12613 DT denotes an
T16929 12614-12621 NN denotes isotype
T16930 12622-12629 NN denotes control
T16931 12630-12638 NN denotes antibody
T16932 12638-12639 . denotes .
T16933 12640-12658 VBN denotes Immunoprecipitated
T16934 12659-12660 -LRB- denotes (
T16935 12660-12662 NNP denotes IP
T16936 12662-12663 -RRB- denotes )
T16937 12664-12672 NN denotes material
T16938 12673-12678 VBN denotes bound
T16939 12679-12681 TO denotes to
T16940 12682-12685 DT denotes the
T16941 12686-12691 NNS denotes beads
T16942 12692-12695 VBD denotes was
T16943 12696-12702 VBN denotes loaded
T16944 12703-12705 IN denotes in
T16945 12706-12711 NNS denotes lanes
T16946 12712-12715 CD denotes 3-5
T16947 12715-12716 . denotes .
T16948 12717-12724 NNS denotes Samples
T16949 12725-12727 IN denotes of
T16950 12728-12731 DT denotes the
T16951 12732-12736 NN denotes cell
T16952 12737-12744 VBZ denotes lysates
T16953 12745-12750 IN denotes after
T16954 12751-12770 NN denotes immunoprecipitation
T16955 12771-12777 VBP denotes appear
T16956 12778-12780 IN denotes in
T16957 12781-12786 NNS denotes lanes
T16958 12787-12788 CD denotes 6
T16959 12789-12790 CD denotes 8
T16960 12790-12791 . denotes .
T16961 12792-12799 JJ denotes Western
T16962 12800-12805 NNS denotes blots
T16963 12806-12810 VBD denotes were
T16964 12811-12817 VBN denotes probed
T16965 12818-12822 IN denotes with
T16966 12823-12833 NNS denotes antibodies
T16967 12834-12842 JJ denotes specific
T16968 12843-12846 IN denotes for
T16969 12847-12852 NNP denotes cIAP1
T16970 12852-12853 , denotes ,
T16971 12854-12859 CD denotes TRAF2
T16972 12859-12860 , denotes ,
T16973 12861-12866 CD denotes TRAF3
T16974 12866-12867 , denotes ,
T16975 12868-12871 CC denotes and
T16976 12872-12876 NNP denotes HOIP
T16977 12877-12878 -LRB- denotes (
T16978 12878-12889 JJ denotes approximate
T16979 12890-12899 JJ denotes molecular
T16980 12900-12907 NNS denotes weights
T16981 12908-12917 VBD denotes indicated
T16982 12918-12920 IN denotes on
T16983 12921-12925 NN denotes left
T16984 12925-12926 -RRB- denotes )
T16985 12926-12927 . denotes .
T16986 12928-12935 JJ denotes Similar
T16987 12936-12943 NNS denotes results
T16988 12944-12948 VBD denotes were
T16989 12949-12957 VBN denotes obtained
T16990 12958-12960 IN denotes in
T16991 12961-12964 CD denotes two
T16992 12965-12975 JJ denotes additional
T16993 12976-12987 NNS denotes experiments
T16994 12987-12988 . denotes .
T16995 12989-12990 -LRB- denotes (
T16996 12990-12991 NNP denotes B
T16997 12991-12992 -RRB- denotes )
T16998 12993-12997 NNP denotes CD40
T16999 12998-13001 VBD denotes was
T17000 13002-13010 VBN denotes isolated
T17001 13011-13013 IN denotes by
T17002 13014-13033 NN denotes immunoprecipitation
T17003 13034-13035 -LRB- denotes (
T17004 13035-13037 IN denotes as
T17005 13038-13040 IN denotes in
T17006 13041-13042 -LRB- denotes (
T17007 13042-13043 NN denotes A
T17008 13043-13044 -RRB- denotes )
T17009 13044-13045 -RRB- denotes )
T17010 13046-13050 IN denotes from
T17011 13051-13054 CD denotes A20
T17012 13054-13056 CD denotes .2
T17013 13056-13057 NNP denotes J
T17014 13058-13063 NNS denotes cells
T17015 13064-13067 CC denotes and
T17016 13068-13082 JJ denotes HOIP-deficient
T17017 13083-13088 NNS denotes cells
T17018 13089-13099 VBN denotes transduced
T17019 13105-13107 DT denotes an
T17020 13108-13113 JJ denotes empty
T17021 13114-13124 NN denotes retroviral
T17023 13132-13133 -LRB- denotes (
T17024 13133-13137 NNP denotes pMIP
T17025 13137-13138 -RRB- denotes )
T17026 13139-13141 CC denotes or
T17027 13142-13143 DT denotes a
T17028 13144-13154 JJ denotes retroviral
T17029 13155-13161 NN denotes vector
T17030 13162-13170 VBG denotes encoding
T17031 13171-13175 NNP denotes HOIP
T17032 13175-13176 . denotes .
T17033 13177-13185 NNP denotes Material
T17034 13186-13204 VBD denotes immunoprecipitated
T17035 13205-13209 IN denotes with
T17036 13210-13212 DT denotes an
T17037 13213-13220 NN denotes isotype
T17038 13221-13228 NN denotes control
T17039 13229-13237 NN denotes antibody
T17040 13238-13239 -LRB- denotes (
T17041 13239-13246 NN denotes isotype
T17042 13246-13247 -RRB- denotes )
T17043 13248-13250 CC denotes or
T17044 13251-13260 JJ denotes anti-CD40
T17045 13261-13269 NN denotes antibody
T17046 13270-13273 VBD denotes was
T17047 13274-13282 VBN denotes examined
T17048 13283-13285 IN denotes by
T17049 13286-13293 JJ denotes Western
T17050 13294-13302 VBG denotes blotting
T17051 13303-13306 IN denotes for
T17052 13307-13311 NNP denotes CD40
T17053 13311-13312 , denotes ,
T17054 13313-13318 CD denotes TRAF2
T17055 13318-13319 , denotes ,
T17056 13320-13325 CD denotes TRAF3
T17057 13325-13326 , denotes ,
T17058 13327-13332 NNP denotes cIAP1
T17059 13332-13333 , denotes ,
T17060 13334-13338 NNP denotes HOIP
T17061 13338-13339 , denotes ,
T17062 13340-13344 NNP denotes IKKα
T17063 13344-13345 NNP denotes /
T17064 13345-13346 NN denotes β
T17065 13346-13347 , denotes ,
T17066 13348-13351 CC denotes and
T17067 13352-13356 NNP denotes IKKγ
T17068 13357-13358 -LRB- denotes (
T17069 13358-13363 JJ denotes right
T17070 13364-13370 NNS denotes panels
T17071 13370-13371 -RRB- denotes )
T17072 13371-13372 . denotes .
T17073 13373-13377 NNP denotes HOIP
T17074 13378-13388 NN denotes expression
T17075 13389-13392 VBD denotes was
T17076 13393-13401 VBN denotes required
T17077 13402-13405 IN denotes for
T17078 13406-13421 NN denotes coprecipitation
T17079 13422-13424 IN denotes of
T17080 13425-13428 NNP denotes IKK
T17081 13429-13437 NNS denotes proteins
T17082 13438-13442 IN denotes with
T17083 13443-13447 NNP denotes CD40
T17084 13447-13448 . denotes .
T17085 13449-13453 NNP denotes Cell
T17086 13454-13461 VBZ denotes lysates
T17087 13462-13466 IN denotes from
T17088 13467-13479 JJ denotes unstimulated
T17089 13480-13485 NNS denotes cells
T17090 13486-13489 VBP denotes are
T17091 13490-13495 VBN denotes shown
T17092 13496-13498 IN denotes in
T17093 13499-13502 DT denotes the
T17094 13503-13507 VBN denotes left
T17095 13508-13514 NNS denotes panels
T17096 13514-13515 . denotes .
T17097 13516-13523 JJ denotes Similar
T17098 13524-13531 NNS denotes results
T17099 13532-13536 VBD denotes were
T17100 13537-13545 VBN denotes obtained
T17101 13546-13548 IN denotes in
T17102 13549-13550 DT denotes a
T17103 13551-13557 JJ denotes second
T17104 13558-13568 NN denotes experiment
T17105 13569-13572 CC denotes and
T17106 13573-13575 IN denotes in
T17107 13576-13579 CD denotes two
T17108 13580-13591 NNS denotes experiments
T17109 13592-13596 IN denotes with
T17110 13597-13598 DT denotes a
T17111 13599-13605 JJ denotes second
T17112 13606-13620 JJ denotes HOIP-deficient
T17113 13621-13626 NN denotes clone
T17114 13626-13627 . denotes .
T17115 13628-13629 -LRB- denotes (
T17116 13629-13630 $ denotes C
T17117 13630-13631 -RRB- denotes )
T17118 14055-14063 VBG denotes blotting
T17120 13635-13642 RBR denotes further
T17121 13643-13651 VB denotes evaluate
T17122 13652-13666 JJ denotes HOIP-dependent
T17123 13667-13678 NN denotes recruitment
T17124 13679-13681 IN denotes of
T17125 13682-13686 NNP denotes IKKγ
T17126 13687-13689 TO denotes to
T17127 13690-13694 NNP denotes CD40
T17128 13694-13695 , denotes ,
T17129 13696-13699 NNP denotes A20
T17130 13699-13701 CD denotes .2
T17131 13701-13702 NNP denotes J
T17132 13703-13708 NNS denotes cells
T17133 13709-13711 CC denotes or
T17134 13712-13726 JJ denotes HOIP-deficient
T17135 13727-13728 -LRB- denotes (
T17136 13728-13732 SYM denotes HOIP
T17137 13732-13733 : denotes -
T17138 13733-13734 SYM denotes /
T17139 13734-13735 : denotes -
T17140 13735-13736 -RRB- denotes )
T17141 13737-13740 CD denotes A20
T17142 13740-13742 CD denotes .2
T17143 13742-13743 NNP denotes J
T17144 13744-13749 NNS denotes cells
T17145 13750-13754 VBD denotes were
T17146 13755-13765 VBN denotes transduced
T17147 13766-13770 IN denotes with
T17148 13771-13773 DT denotes an
T17149 13774-13779 JJ denotes empty
T17150 13780-13790 NN denotes retroviral
T17151 13791-13797 NN denotes vector
T17152 13798-13800 CC denotes or
T17153 13801-13802 DT denotes a
T17154 13803-13813 JJ denotes retroviral
T17155 13814-13823 VBP denotes construct
T17156 13824-13832 VBG denotes encoding
T17157 13833-13835 NNP denotes BP
T17158 13836-13850 JJ denotes epitope-tagged
T17159 13851-13855 NNP denotes IKKγ
T17160 13856-13857 -LRB- denotes (
T17161 13857-13862 VBN denotes noted
T17162 13863-13865 IN denotes in
T17163 13866-13869 DT denotes the
T17164 13870-13876 NN denotes figure
T17165 13877-13879 IN denotes as
T17166 13880-13884 NNP denotes pMIP
T17167 13885-13888 CC denotes and
T17168 13889-13893 NNP denotes IKKγ
T17169 13893-13894 , denotes ,
T17170 13895-13907 RB denotes respectively
T17171 13907-13908 -RRB- denotes )
T17172 13908-13909 . denotes .
T17173 13910-13917 NNP denotes Lysates
T17174 13918-13919 -LRB- denotes (
T17175 13919-13924 NNS denotes lanes
T17176 13925-13926 CD denotes 1
T17177 13927-13928 LS denotes 3
T17178 13928-13929 -RRB- denotes )
T17179 13930-13933 CC denotes and
T17180 13934-13953 NN denotes immunoprecipitation
T17181 13954-13955 -LRB- denotes (
T17182 13955-13957 NNP denotes IP
T17183 13957-13958 -RRB- denotes )
T17184 13959-13966 NNS denotes samples
T17185 13967-13968 -LRB- denotes (
T17186 13968-13973 NNS denotes lanes
T17187 13974-13975 CD denotes 4
T17188 13976-13978 CD denotes 11
T17189 13978-13979 -RRB- denotes )
T17190 13980-13984 IN denotes from
T17191 13985-13988 DT denotes the
T17192 13989-13993 NN denotes cell
T17193 13994-13999 NNS denotes lines
T17194 14000-14004 VBD denotes were
T17195 14005-14017 VBN denotes fractionated
T17196 14018-14020 IN denotes by
T17197 14021-14029 NNP denotes SDS-PAGE
T17198 14030-14033 CC denotes and
T17199 14034-14043 VBN denotes evaluated
T17200 14044-14046 IN denotes by
T17201 14047-14054 JJ denotes Western
T17202 14064-14068 IN denotes with
T17203 14069-14079 NNS denotes antibodies
T17204 14080-14082 TO denotes to
T17205 14083-14086 DT denotes the
T17206 14087-14089 NNP denotes BP
T17207 14090-14093 NN denotes tag
T17208 14094-14095 -LRB- denotes (
T17209 14095-14099 NNP denotes IKKγ
T17210 14099-14100 , denotes ,
T17211 14101-14106 JJ denotes upper
T17212 14107-14112 NN denotes panel
T17213 14112-14113 -RRB- denotes )
T17214 14114-14117 CC denotes and
T17215 14118-14123 CD denotes TRAF2
T17216 14123-14124 . denotes .
T17217 14125-14128 DT denotes The
T17218 14129-14138 JJ denotes anti-CD40
T17219 14139-14141 NNP denotes IP
T17220 14142-14148 NN denotes sample
T17221 14149-14151 IN denotes in
T17222 14152-14156 NN denotes lane
T17223 14157-14159 CD denotes 11
T17224 14160-14163 VBD denotes was
T17225 14164-14171 VBN denotes treated
T17226 14172-14176 IN denotes with
T17227 14177-14178 JJ denotes λ
T17228 14179-14190 NN denotes phosphatase
T17229 14190-14191 : denotes ;
T17230 14192-14195 DT denotes the
T17231 14196-14202 NN denotes sample
T17232 14203-14205 IN denotes in
T17233 14206-14210 NN denotes lane
T17234 14211-14213 CD denotes 10
T17235 14214-14217 VBD denotes was
T17236 14218-14230 JJ denotes mock-treated
T17237 14230-14231 . denotes .
T17238 14232-14239 NN denotes Protein
T17239 14240-14247 NNS denotes samples
T17240 14248-14249 -LRB- denotes (
T17241 14249-14254 CC denotes minus
T17242 14255-14260 DT denotes those
T17243 14261-14268 VBN denotes treated
T17244 14269-14273 IN denotes with
T17245 14274-14285 NN denotes phosphatase
T17246 14285-14286 -RRB- denotes )
T17247 14287-14291 VBD denotes were
T17248 14292-14296 RB denotes also
T17249 14297-14309 VBN denotes fractionated
T17250 14310-14312 IN denotes on
T17251 14313-14314 DT denotes a
T17252 14315-14323 JJ denotes separate
T17253 14324-14327 NN denotes gel
T17254 14328-14329 -LRB- denotes (
T17255 14329-14334 JJR denotes lower
T17256 14335-14345 NN denotes acrylamide
T17257 14346-14359 NN denotes concentration
T17258 14359-14360 -RRB- denotes )
T17259 14361-14364 IN denotes for
T17260 14365-14368 DT denotes the
T17261 14369-14379 NN denotes evaluation
T17262 14380-14382 IN denotes of
T17263 14383-14388 CD denotes TRAF3
T17264 14389-14392 CC denotes and
T17265 14393-14397 NNP denotes HOIP
T17266 14398-14399 -LRB- denotes (
T17267 14399-14405 JJ denotes bottom
T17268 14406-14409 CD denotes two
T17269 14410-14416 NNS denotes panels
T17270 14416-14417 -RRB- denotes )
T17271 14417-14418 . denotes .
T17272 14419-14426 JJ denotes Similar
T17273 14427-14434 NNS denotes results
T17274 14435-14439 VBD denotes were
T17275 14440-14448 VBN denotes obtained
T17276 14449-14451 IN denotes in
T17277 14452-14455 CD denotes two
T17278 14456-14466 JJ denotes additional
T17279 14467-14478 NNS denotes experiments
T17280 14478-14479 . denotes .
T16917 12545-12549 VBD denotes were
T17022 13125-13131 NN denotes vector
T17119 13632-13634 TO denotes To
R2125 T2880 T2880 ROOT Generation,Generation
R2126 T2881 T2880 prep of,Generation
R2127 T2882 T2883 compound HOIP-deficient,B
R2128 T2883 T2884 compound B,cells
R2129 T2884 T2881 pobj cells,of
R2130 T2885 T2880 prep via,Generation
R2131 T2886 T2887 amod targeted,disruption
R2132 T2887 T2885 pobj disruption,via
R2133 T2888 T2887 prep of,disruption
R2134 T2889 T2888 pobj Rnf31,of
R2135 T2890 T2889 punct ",",Rnf31
R2136 T2891 T2892 det the,gene
R2137 T2892 T2889 appos gene,Rnf31
R2138 T2893 T2892 acl encoding,gene
R2139 T2894 T2893 dobj HOIP,encoding
R2140 T2895 T2901 prep In,demonstrated
R2141 T2896 T2898 poss our,study
R2142 T2897 T2898 amod previous,study
R2143 T2898 T2895 pobj study,In
R2144 T2899 T2901 punct ",",demonstrated
R2145 T2900 T2901 nsubj we,demonstrated
R2146 T2901 T2901 ROOT demonstrated,demonstrated
R2147 T2902 T2905 mark that,recruited
R2148 T2903 T2905 nsubjpass HOIP,recruited
R2149 T2904 T2905 auxpass is,recruited
R2150 T2905 T2901 ccomp recruited,demonstrated
R2151 T2906 T2905 prep to,recruited
R2152 T2907 T2908 det the,CD40
R2153 T2908 T2909 nsubj CD40,signaling
R2154 T2909 T2906 pcomp signaling,to
R2155 T2910 T2909 dobj complex,signaling
R2156 T2911 T2909 prep in,signaling
R2157 T2912 T2916 nummod two,lines
R2158 T2913 T2915 compound mouse,cell
R2159 T2914 T2915 compound B,cell
R2160 T2915 T2916 compound cell,lines
R2161 T2916 T2911 pobj lines,in
R2162 T2917 T2906 pobj [,to
R2163 T2918 T2919 nummod 6,]
R2164 T2919 T2906 pobj ],to
R2165 T2920 T2901 punct .,demonstrated
R2166 T2921 T2922 amod Similar,results
R2167 T2922 T2924 nsubjpass results,obtained
R2168 T2923 T2924 auxpass were,obtained
R2169 T2924 T2924 ROOT obtained,obtained
R2170 T2925 T2924 prep with,obtained
R2171 T2926 T2927 compound mouse,splenocytes
R2172 T2927 T2925 pobj splenocytes,with
R2173 T2928 T2924 punct ",",obtained
R2174 T2929 T2924 advcl indicating,obtained
R2175 T2930 T2939 mark that,limited
R2176 T2931 T2932 det the,interaction
R2177 T2932 T2939 nsubjpass interaction,limited
R2178 T2933 T2932 prep of,interaction
R2179 T2934 T2933 pobj CD40,of
R2180 T2935 T2932 prep with,interaction
R2181 T2936 T2935 pobj HOIP,with
R2182 T2937 T2939 auxpass is,limited
R2183 T2938 T2939 neg not,limited
R2184 T2939 T2929 ccomp limited,indicating
R2185 T2940 T2941 aux to,transformed
R2186 T2941 T2939 xcomp transformed,limited
R2187 T2942 T2943 compound B,cell
R2188 T2943 T2944 compound cell,lines
R2189 T2944 T2941 dobj lines,transformed
R2190 T2945 T2944 punct (,lines
R2191 T2946 T2947 amod unpublished,observations
R2192 T2947 T2944 appos observations,lines
R2193 T2948 T2944 punct ),lines
R2194 T2949 T2924 punct .,obtained
R2195 T2950 T2952 nsubj We,found
R2196 T2951 T2952 advmod also,found
R2197 T2952 T2952 ROOT found,found
R2198 T2953 T2960 mark that,was
R2199 T2954 T2955 det the,recruitment
R2200 T2955 T2960 nsubj recruitment,was
R2201 T2956 T2955 prep of,recruitment
R2202 T2957 T2956 pobj HOIP,of
R2203 T2958 T2955 prep to,recruitment
R2204 T2959 T2958 pobj CD40,to
R2205 T2960 T2952 ccomp was,found
R2206 T2961 T2960 acomp TRAF2-dependent,was
R2207 T2962 T2960 cc and,was
R2208 T2963 T2964 det that,overexpression
R2209 T2964 T2960 conj overexpression,was
R2210 T2965 T2964 prep of,overexpression
R2211 T2966 T2969 det a,mutant
R2212 T2967 T2969 amod truncated,mutant
R2213 T2968 T2969 compound HOIP,mutant
R2214 T2969 T2974 amod mutant,activation
R2215 T2970 T2971 advmod partially,inhibited
R2216 T2971 T2974 amod inhibited,activation
R2217 T2972 T2974 amod CD40-mediated,activation
R2218 T2973 T2974 compound NF-κB,activation
R2219 T2974 T2965 pobj activation,of
R2220 T2975 T2952 punct .,found
R2221 T2976 T2977 det These,results
R2222 T2977 T2978 nsubj results,support
R2223 T2978 T2978 ROOT support,support
R2224 T2979 T2980 det the,hypothesis
R2225 T2980 T2978 dobj hypothesis,support
R2226 T2981 T2983 mark that,plays
R2227 T2982 T2983 nsubj HOIP,plays
R2228 T2983 T2980 acl plays,hypothesis
R2229 T2984 T2985 det a,role
R2230 T2985 T2983 dobj role,plays
R2231 T2986 T2985 prep in,role
R2232 T2987 T2989 nummod CD40,transduction
R2233 T2988 T2989 compound signal,transduction
R2234 T2989 T2986 pobj transduction,in
R2235 T2990 T2978 punct .,support
R2236 T2991 T2993 aux To,define
R2237 T2992 T2993 advmod further,define
R2238 T2993 T3007 advcl define,used
R2239 T2994 T2995 det the,role
R2240 T2995 T2993 dobj role,define
R2241 T2996 T2993 cc and,define
R2242 T2997 T2993 conj evaluate,define
R2243 T2998 T2999 det the,importance
R2244 T2999 T2997 dobj importance,evaluate
R2245 T3000 T2999 prep of,importance
R2246 T3001 T3000 pobj HOIP,of
R2247 T3002 T2997 prep in,evaluate
R2248 T3003 T3002 pobj CD40,in
R2249 T3004 T2997 advcl signaling,evaluate
R2250 T3005 T3007 punct ",",used
R2251 T3006 T3007 nsubj we,used
R2252 T3007 T3007 ROOT used,used
R2253 T3008 T3010 compound somatic,gene
R2254 T3009 T3010 compound cell,gene
R2255 T3010 T3007 dobj gene,used
R2256 T3011 T3010 acl targeting,gene
R2257 T3012 T3013 aux to,disrupt
R2258 T3013 T3011 xcomp disrupt,targeting
R2259 T3014 T3015 det the,gene
R2260 T3015 T3013 dobj gene,disrupt
R2261 T3016 T3015 acl encoding,gene
R2262 T3017 T3016 dobj HOIP,encoding
R2263 T3018 T3016 prep in,encoding
R2264 T3019 T3023 det the,line
R2265 T3020 T3022 compound mouse,cell
R2266 T3021 T3022 compound B,cell
R2267 T3022 T3023 compound cell,line
R2268 T3023 T3018 pobj line,in
R2269 T3024 T3026 nmod A20,J
R2270 T3025 T3026 nummod .2,J
R2271 T3026 T3023 appos J,line
R2272 T3027 T3007 punct .,used
R2273 T3028 T3030 det This,line
R2274 T3029 T3030 compound cell,line
R2275 T3030 T3032 nsubj line,been
R2276 T3031 T3032 aux has,been
R2277 T3032 T3032 ROOT been,been
R2278 T3033 T3034 advmod particularly,useful
R2279 T3034 T3032 acomp useful,been
R2280 T3035 T3034 prep in,useful
R2281 T3036 T3037 det the,characterization
R2282 T3037 T3035 pobj characterization,in
R2283 T3038 T3037 prep of,characterization
R2284 T3039 T3038 pobj CD40,of
R2285 T3040 T3032 advcl signaling,been
R2286 T3041 T3040 dobj mechanisms,signaling
R2287 T3042 T3041 amod due,mechanisms
R2288 T3043 T3042 pcomp to,due
R2289 T3044 T3046 det the,ease
R2290 T3045 T3046 amod relative,ease
R2291 T3046 T3042 pobj ease,due
R2292 T3047 T3053 prep with,modified
R2293 T3048 T3047 pobj which,with
R2294 T3049 T3050 poss its,genome
R2295 T3050 T3053 nsubjpass genome,modified
R2296 T3051 T3053 aux can,modified
R2297 T3052 T3053 auxpass be,modified
R2298 T3053 T3046 relcl modified,ease
R2299 T3054 T3053 agent by,modified
R2300 T3055 T3057 nmod homologous,[
R2301 T3056 T3057 compound recombination,[
R2302 T3057 T3059 nmod [,]
R2303 T3058 T3059 nummod 7,]
R2304 T3059 T3054 pobj ],by
R2305 T3060 T3059 punct ",",]
R2306 T3061 T3063 nmod [,]
R2307 T3062 T3063 nummod 8,]
R2308 T3063 T3059 conj ],]
R2309 T3064 T3063 punct ",",]
R2310 T3065 T3067 det [,]
R2311 T3066 T3067 nummod 9,]
R2312 T3067 T3063 appos ],]
R2313 T3068 T3032 punct .,been
R2314 T3069 T3070 nsubj We,used
R2315 T3070 T3070 ROOT used,used
R2316 T3071 T3073 det a,vector
R2317 T3072 T3073 compound targeting,vector
R2318 T3073 T3070 dobj vector,used
R2319 T3074 T3073 amod capable,vector
R2320 T3075 T3074 prep of,capable
R2321 T3076 T3075 pcomp undergoing,of
R2322 T3077 T3078 amod homologous,recombination
R2323 T3078 T3076 dobj recombination,undergoing
R2324 T3079 T3076 prep with,undergoing
R2325 T3080 T3079 pobj Rnf31,with
R2326 T3081 T3080 punct (,Rnf31
R2327 T3082 T3083 det the,gene
R2328 T3083 T3080 appos gene,Rnf31
R2329 T3084 T3083 acl encoding,gene
R2330 T3085 T3084 dobj HOIP,encoding
R2331 T3086 T3080 punct ),Rnf31
R2332 T3087 T3088 aux to,disrupt
R2333 T3088 T3076 advcl disrupt,undergoing
R2334 T3089 T3091 det the,sequence
R2335 T3090 T3091 compound coding,sequence
R2336 T3091 T3088 dobj sequence,disrupt
R2337 T3092 T3091 prep of,sequence
R2338 T3093 T3094 det the,gene
R2339 T3094 T3092 pobj gene,of
R2340 T3095 T3088 prep in,disrupt
R2341 T3096 T3095 pobj exon,in
R2342 T3097 T3096 nummod 5,exon
R2343 T3098 T3096 punct (,exon
R2344 T3099 T3100 compound Fig.,1A
R2345 T3100 T3096 appos 1A,exon
R2346 T3101 T3096 punct ),exon
R2347 T3102 T3070 punct .,used
R2348 T3103 T3115 prep Following,screened
R2349 T3104 T3103 pobj introduction,Following
R2350 T3105 T3104 prep of,introduction
R2351 T3106 T3107 det the,vector
R2352 T3107 T3105 pobj vector,of
R2353 T3108 T3115 punct ",",screened
R2354 T3109 T3111 det the,clones
R2355 T3110 T3111 amod neomycin-resistant,clones
R2356 T3111 T3115 nsubjpass clones,screened
R2357 T3112 T3113 nsubj that,arose
R2358 T3113 T3111 relcl arose,clones
R2359 T3114 T3115 auxpass were,screened
R2360 T3115 T3115 ROOT screened,screened
R2361 T3116 T3115 agent by,screened
R2362 T3117 T3118 compound PCR,amplification
R2363 T3118 T3116 pobj amplification,by
R2364 T3119 T3118 prep of,amplification
R2365 T3120 T3121 amod genomic,DNA
R2366 T3121 T3119 pobj DNA,of
R2367 T3122 T3123 aux to,identify
R2368 T3123 T3115 xcomp identify,screened
R2369 T3124 T3123 dobj cells,identify
R2370 T3125 T3124 acl containing,cells
R2371 T3126 T3129 det a,allele
R2372 T3127 T3129 amod disrupted,allele
R2373 T3128 T3129 compound Rnf31,allele
R2374 T3129 T3125 dobj allele,containing
R2375 T3130 T3115 punct .,screened
R2376 T3131 T3132 aux To,remove
R2377 T3132 T3149 advcl remove,transfected
R2378 T3133 T3137 det the,cassette
R2379 T3134 T3136 amod selectable,gene
R2380 T3135 T3136 compound marker,gene
R2381 T3136 T3137 compound gene,cassette
R2382 T3137 T3132 dobj cassette,remove
R2383 T3138 T3132 prep from,remove
R2384 T3139 T3142 det the,allele
R2385 T3140 T3142 amod disrupted,allele
R2386 T3141 T3142 compound Rnf31,allele
R2387 T3142 T3138 pobj allele,from
R2388 T3143 T3149 punct ",",transfected
R2389 T3144 T3146 amod recombinant,lines
R2390 T3145 T3146 compound cell,lines
R2391 T3146 T3149 nsubjpass lines,transfected
R2392 T3147 T3149 auxpass were,transfected
R2393 T3148 T3149 advmod transiently,transfected
R2394 T3149 T3149 ROOT transfected,transfected
R2395 T3150 T3149 prep with,transfected
R2396 T3151 T3152 det a,plasmid
R2397 T3152 T3150 pobj plasmid,with
R2398 T3153 T3154 nsubj that,encodes
R2399 T3154 T3152 relcl encodes,plasmid
R2400 T3155 T3156 compound Cre,recombinase
R2401 T3156 T3154 dobj recombinase,encodes
R2402 T3157 T3149 punct .,transfected
R2403 T3158 T3159 det This,step
R2404 T3159 T3160 nsubj step,allowed
R2405 T3160 T3160 ROOT allowed,allowed
R2406 T3161 T3163 nsubj us,perform
R2407 T3162 T3163 aux to,perform
R2408 T3163 T3160 ccomp perform,allowed
R2409 T3164 T3166 det a,round
R2410 T3165 T3166 amod second,round
R2411 T3166 T3163 dobj round,perform
R2412 T3167 T3166 prep of,round
R2413 T3168 T3167 pobj targeting,of
R2414 T3169 T3168 cc and,targeting
R2415 T3170 T3171 compound drug,selection
R2416 T3171 T3168 conj selection,targeting
R2417 T3172 T3171 punct ",",selection
R2418 T3173 T3168 conj generating,targeting
R2419 T3174 T3173 dobj cells,generating
R2420 T3175 T3182 prep in,disrupted
R2421 T3176 T3175 pobj which,in
R2422 T3177 T3178 det both,copies
R2423 T3178 T3182 nsubjpass copies,disrupted
R2424 T3179 T3178 prep of,copies
R2425 T3180 T3179 pobj Rnf31,of
R2426 T3181 T3182 auxpass were,disrupted
R2427 T3182 T3174 relcl disrupted,cells
R2428 T3183 T3160 punct .,allowed
R2429 T3184 T3188 nummod Two,lines
R2430 T3185 T3188 amod independent,lines
R2431 T3186 T3188 amod clonal,lines
R2432 T3187 T3188 compound cell,lines
R2433 T3188 T3190 nsubjpass lines,chosen
R2434 T3189 T3190 auxpass were,chosen
R2435 T3190 T3190 ROOT chosen,chosen
R2436 T3191 T3190 prep for,chosen
R2437 T3192 T3193 amod further,analysis
R2438 T3193 T3191 pobj analysis,for
R2439 T3194 T3190 punct .,chosen
R2440 T3195 T3197 compound HOIP,expression
R2441 T3196 T3197 compound protein,expression
R2442 T3197 T3198 nsubj expression,was
R2443 T3198 T3198 ROOT was,was
R2444 T3199 T3198 acomp undetectable,was
R2445 T3200 T3199 prep in,undetectable
R2446 T3201 T3203 det both,lines
R2447 T3202 T3203 compound cell,lines
R2448 T3203 T3200 pobj lines,in
R2449 T3204 T3198 punct ",",was
R2450 T3205 T3206 mark as,determined
R2451 T3206 T3198 advcl determined,was
R2452 T3207 T3206 agent by,determined
R2453 T3208 T3210 amod Western,analysis
R2454 T3209 T3210 compound blot,analysis
R2455 T3210 T3207 pobj analysis,by
R2456 T3211 T3210 prep of,analysis
R2457 T3212 T3213 compound cell,lysates
R2458 T3213 T3211 pobj lysates,of
R2459 T3214 T3210 punct (,analysis
R2460 T3215 T3216 compound Fig.,1B
R2461 T3216 T3210 appos 1B,analysis
R2462 T3217 T3210 punct ),analysis
R2463 T3218 T3198 punct ",",was
R2464 T3219 T3198 advcl demonstrating,was
R2465 T3220 T3224 mark that,was
R2466 T3221 T3223 det the,process
R2467 T3222 T3223 compound targeting,process
R2468 T3223 T3224 nsubj process,was
R2469 T3224 T3219 ccomp was,demonstrating
R2470 T3225 T3224 acomp successful,was
R2471 T3226 T3198 punct .,was
R2472 T3227 T3239 prep In,referred
R2473 T3228 T3229 det the,text
R2474 T3229 T3227 pobj text,In
R2475 T3230 T3231 nsubj that,follows
R2476 T3231 T3229 relcl follows,text
R2477 T3232 T3239 punct ",",referred
R2478 T3233 T3237 det the,lines
R2479 T3234 T3237 nummod two,lines
R2480 T3235 T3237 amod gene-targeted,lines
R2481 T3236 T3237 compound cell,lines
R2482 T3237 T3239 nsubjpass lines,referred
R2483 T3238 T3239 auxpass are,referred
R2484 T3239 T3239 ROOT referred,referred
R2485 T3240 T3239 prep to,referred
R2486 T3241 T3239 prep as,referred
R2487 T3242 T3243 amod HOIP-deficient,cells
R2488 T3243 T3241 pobj cells,as
R2489 T3244 T3239 punct .,referred
R2490 T3245 T3246 aux To,enable
R2491 T3246 T3274 advcl enable,transduced
R2492 T3247 T3246 dobj us,enable
R2493 T3248 T3249 aux to,confirm
R2494 T3249 T3246 xcomp confirm,enable
R2495 T3250 T3260 mark that,were
R2496 T3251 T3252 det any,signaling
R2497 T3252 T3260 nsubj signaling,were
R2498 T3253 T3252 cc or,signaling
R2499 T3254 T3255 amod functional,defects
R2500 T3255 T3256 nsubj defects,observed
R2501 T3256 T3252 conj observed,signaling
R2502 T3257 T3256 prep in,observed
R2503 T3258 T3259 amod HOIP-deficient,cells
R2504 T3259 T3257 pobj cells,in
R2505 T3260 T3249 ccomp were,confirm
R2506 T3261 T3260 acomp due,were
R2507 T3262 T3261 prep to,due
R2508 T3263 T3262 pobj disruption,to
R2509 T3264 T3263 prep of,disruption
R2510 T3265 T3267 det the,gene
R2511 T3266 T3267 compound Rnf31,gene
R2512 T3267 T3264 pobj gene,of
R2513 T3268 T3274 punct ",",transduced
R2514 T3269 T3272 det both,lines
R2515 T3270 T3272 amod HOIP-deficient,lines
R2516 T3271 T3272 compound cell,lines
R2517 T3272 T3274 nsubjpass lines,transduced
R2518 T3273 T3274 auxpass were,transduced
R2519 T3274 T3274 ROOT transduced,transduced
R2520 T3275 T3274 prep with,transduced
R2521 T3276 T3277 det a,retrovirus
R2522 T3277 T3278 nsubj retrovirus,encoding
R2523 T3278 T3281 amod encoding,HOIP
R2524 T3279 T3281 compound FLAG-tagged,HOIP
R2525 T3280 T3281 compound wild-type,HOIP
R2526 T3281 T3275 pobj HOIP,with
R2527 T3282 T3274 punct ",",transduced
R2528 T3283 T3284 advmod thus,restoring
R2529 T3284 T3274 advcl restoring,transduced
R2530 T3285 T3287 compound HOIP,expression
R2531 T3286 T3287 compound protein,expression
R2532 T3287 T3284 dobj expression,restoring
R2533 T3288 T3290 punct (,1B
R2534 T3289 T3290 compound Fig.,1B
R2535 T3290 T3284 appos 1B,restoring
R2536 T3291 T3290 punct ),1B
R2537 T3292 T3274 punct .,transduced
R2538 T3293 T3294 det These,cells
R2539 T3294 T3296 nsubjpass cells,referred
R2540 T3295 T3296 auxpass are,referred
R2541 T3296 T3296 ROOT referred,referred
R2542 T3297 T3296 prep to,referred
R2543 T3298 T3296 prep as,referred
R2544 T3299 T3300 amod HOIP-reconstituted,cells
R2545 T3300 T3298 pobj cells,as
R2546 T3301 T3296 punct .,referred
R2547 T3302 T3313 prep In,compared
R2548 T3303 T3304 det the,experiments
R2549 T3304 T3302 pobj experiments,In
R2550 T3305 T3306 nsubj that,follow
R2551 T3306 T3304 relcl follow,experiments
R2552 T3307 T3313 punct ",",compared
R2553 T3308 T3313 nsubjpass responses,compared
R2554 T3309 T3308 prep by,responses
R2555 T3310 T3311 det these,cells
R2556 T3311 T3309 pobj cells,by
R2557 T3312 T3313 auxpass were,compared
R2558 T3313 T3313 ROOT compared,compared
R2559 T3314 T3313 prep to,compared
R2560 T3315 T3314 pobj those,to
R2561 T3316 T3315 prep of,those
R2562 T3317 T3320 compound A20,cells
R2563 T3318 T3319 compound .2,J
R2564 T3319 T3320 compound J,cells
R2565 T3320 T3316 pobj cells,of
R2566 T3321 T3320 cc and,cells
R2567 T3322 T3323 amod HOIP-deficient,cells
R2568 T3323 T3320 conj cells,cells
R2569 T3324 T3315 acl transduced,those
R2570 T3325 T3324 prep with,transduced
R2571 T3326 T3328 det a,vector
R2572 T3327 T3328 amod retroviral,vector
R2573 T3328 T3325 pobj vector,with
R2574 T3329 T3328 punct (,vector
R2575 T3330 T3328 appos pMIP,vector
R2576 T3331 T3328 punct ),vector
R2577 T3332 T3315 acl lacking,those
R2578 T3333 T3335 det a,insert
R2579 T3334 T3335 compound cDNA,insert
R2580 T3335 T3332 dobj insert,lacking
R2581 T3336 T3335 punct (,insert
R2582 T3337 T3338 amod empty,vector
R2583 T3338 T3335 appos vector,insert
R2584 T3339 T3335 punct ),insert
R2585 T3340 T3313 punct .,compared
R3249 T4259 T4261 nsubjpass HOIP,required
R3250 T4260 T4261 auxpass is,required
R3251 T4261 T4261 ROOT required,required
R3252 T4262 T4261 prep for,required
R3253 T4263 T4265 amod CD40-mediated,upregulation
R3254 T4264 T4265 compound CD80,upregulation
R3255 T4265 T4262 pobj upregulation,for
R3256 T4266 T4265 cc and,upregulation
R3257 T4267 T4265 conj activation,upregulation
R3258 T4268 T4267 prep of,activation
R3259 T4269 T4271 compound germline,transcription
R3260 T4270 T4271 compound epsilon,transcription
R3261 T4271 T4268 pobj transcription,of
R3262 T4272 T4265 conj Engagement,upregulation
R3263 T4273 T4272 prep of,Engagement
R3264 T4274 T4273 pobj CD40,of
R3265 T4275 T4272 prep on,Engagement
R3266 T4276 T4277 compound B,cells
R3267 T4277 T4275 pobj cells,on
R3268 T4278 T4279 compound upregulates,expression
R3269 T4279 T4261 dobj expression,required
R3270 T4280 T4279 prep of,expression
R3271 T4281 T4282 compound CD80,[
R3272 T4282 T4280 pobj [,of
R3273 T4283 T4284 nummod 10,]
R3274 T4284 T4279 appos ],expression
R3275 T4285 T4284 punct ",",]
R3276 T4286 T4289 det a,protein
R3277 T4287 T4288 compound cell,surface
R3278 T4288 T4289 compound surface,protein
R3279 T4289 T4279 appos protein,expression
R3280 T4290 T4291 nsubj that,promotes
R3281 T4291 T4289 relcl promotes,protein
R3282 T4292 T4291 dobj activation,promotes
R3283 T4293 T4292 prep of,activation
R3284 T4294 T4295 compound T,cells
R3285 T4295 T4293 pobj cells,of
R3286 T4296 T4295 acl interacting,cells
R3287 T4297 T4296 prep with,interacting
R3288 T4298 T4299 compound B,cells
R3289 T4299 T4297 pobj cells,with
R3290 T4300 T4299 cc and,cells
R3291 T4301 T4302 amod other,APC
R3292 T4302 T4299 conj APC,cells
R3293 T4303 T4261 punct .,required
R3294 T4304 T4305 aux To,begin
R3295 T4305 T4316 advcl begin,assayed
R3296 T4306 T4305 xcomp evaluating,begin
R3297 T4307 T4308 amod potential,contributions
R3298 T4308 T4306 dobj contributions,evaluating
R3299 T4309 T4308 prep of,contributions
R3300 T4310 T4309 pobj HOIP,of
R3301 T4311 T4306 prep to,evaluating
R3302 T4312 T4313 nsubj CD40,signaling
R3303 T4313 T4311 pcomp signaling,to
R3304 T4314 T4316 punct ",",assayed
R3305 T4315 T4316 nsubj we,assayed
R3306 T4316 T4316 ROOT assayed,assayed
R3307 T4317 T4318 det the,upregulation
R3308 T4318 T4316 dobj upregulation,assayed
R3309 T4319 T4318 prep of,upregulation
R3310 T4320 T4319 pobj CD80,of
R3311 T4321 T4318 acl induced,upregulation
R3312 T4322 T4321 agent by,induced
R3313 T4323 T4322 pobj engagement,by
R3314 T4324 T4323 prep of,engagement
R3315 T4325 T4324 pobj CD40,of
R3316 T4326 T4323 prep on,engagement
R3317 T4327 T4330 amod parental,J
R3318 T4328 T4330 nmod A20,J
R3319 T4329 T4330 nummod .2,J
R3320 T4330 T4326 pobj J,on
R3321 T4331 T4330 punct ",",J
R3322 T4332 T4330 conj HOIP-deficient,J
R3323 T4333 T4332 punct ",",HOIP-deficient
R3324 T4334 T4332 cc and,HOIP-deficient
R3325 T4335 T4336 amod HOIP-reconstituted,cells
R3326 T4336 T4332 conj cells,HOIP-deficient
R3327 T4337 T4338 punct (,Fig.
R3328 T4338 T4336 parataxis Fig.,cells
R3329 T4339 T4338 nummod 2,Fig.
R3330 T4340 T4338 punct ),Fig.
R3331 T4341 T4316 punct .,assayed
R3332 T4342 T4356 prep Compared,reduced
R3333 T4343 T4342 prep to,Compared
R3334 T4344 T4347 det the,line
R3335 T4345 T4347 amod parental,line
R3336 T4346 T4347 compound cell,line
R3337 T4347 T4343 pobj line,to
R3338 T4348 T4347 punct ",",line
R3339 T4349 T4351 det the,upregulation
R3340 T4350 T4351 amod CD40-stimulated,upregulation
R3341 T4351 T4356 nsubjpass upregulation,reduced
R3342 T4352 T4351 prep of,upregulation
R3343 T4353 T4352 pobj CD80,of
R3344 T4354 T4356 auxpass was,reduced
R3345 T4355 T4356 advmod dramatically,reduced
R3346 T4356 T4356 ROOT reduced,reduced
R3347 T4357 T4356 prep in,reduced
R3348 T4358 T4360 det all,cells
R3349 T4359 T4360 amod HOIP-deficient,cells
R3350 T4360 T4357 pobj cells,in
R3351 T4361 T4356 conj tested,reduced
R3352 T4362 T4356 punct .,reduced
R3353 T4363 T4370 prep In,restored
R3354 T4364 T4363 pobj contrast,In
R3355 T4365 T4370 punct ",",restored
R3356 T4366 T4370 nsubjpass upregulation,restored
R3357 T4367 T4366 prep of,upregulation
R3358 T4368 T4367 pobj CD80,of
R3359 T4369 T4370 auxpass was,restored
R3360 T4370 T4370 ROOT restored,restored
R3361 T4371 T4370 prep in,restored
R3362 T4372 T4373 amod HOIP-reconstituted,cells
R3363 T4373 T4371 pobj cells,in
R3364 T4374 T4370 punct ",",restored
R3365 T4375 T4370 advcl confirming,restored
R3366 T4376 T4386 mark that,was
R3367 T4377 T4378 det the,defect
R3368 T4378 T4386 nsubj defect,was
R3369 T4379 T4378 prep in,defect
R3370 T4380 T4381 compound CD80,upregulation
R3371 T4381 T4379 pobj upregulation,in
R3372 T4382 T4381 prep in,upregulation
R3373 T4383 T4385 det the,cells
R3374 T4384 T4385 amod HOIP-deficient,cells
R3375 T4385 T4382 pobj cells,in
R3376 T4386 T4375 ccomp was,confirming
R3377 T4387 T4386 acomp due,was
R3378 T4388 T4387 prep to,due
R3379 T4389 T4390 det the,disruption
R3380 T4390 T4388 pobj disruption,to
R3381 T4391 T4390 prep of,disruption
R3382 T4392 T4391 pobj Rnf31,of
R3383 T4393 T4370 punct .,restored
R3384 T4394 T4395 nummod CD40,signals
R3385 T4395 T4401 nsubj signals,contribute
R3386 T4396 T4395 prep in,signals
R3387 T4397 T4398 compound B,cells
R3388 T4398 T4396 pobj cells,in
R3389 T4399 T4401 aux can,contribute
R3390 T4400 T4401 advmod also,contribute
R3391 T4401 T4401 ROOT contribute,contribute
R3392 T4402 T4401 prep to,contribute
R3393 T4403 T4404 det the,activation
R3394 T4404 T4402 pobj activation,to
R3395 T4405 T4404 prep of,activation
R3396 T4406 T4407 compound DNA,recombination
R3397 T4407 T4405 pobj recombination,of
R3398 T4408 T4404 prep in,activation
R3399 T4409 T4413 det the,locus
R3400 T4410 T4413 compound immunoglobulin,locus
R3401 T4411 T4412 amod heavy,chain
R3402 T4412 T4413 compound chain,locus
R3403 T4413 T4408 pobj locus,in
R3404 T4414 T4416 compound [,]
R3405 T4415 T4416 compound 11,]
R3406 T4416 T4404 appos ],activation
R3407 T4417 T4416 punct ",",]
R3408 T4418 T4420 nmod [,]
R3409 T4419 T4420 nummod 12,]
R3410 T4420 T4416 appos ],]
R3411 T4421 T4401 punct .,contribute
R3412 T4422 T4423 det This,process
R3413 T4423 T4436 nsubj process,allows
R3414 T4424 T4423 punct ",",process
R3415 T4425 T4423 acl known,process
R3416 T4426 T4425 prep as,known
R3417 T4427 T4429 amod immunoglobulin,switching
R3418 T4428 T4429 compound class,switching
R3419 T4429 T4426 pobj switching,as
R3420 T4430 T4429 punct (,switching
R3421 T4431 T4429 cc or,switching
R3422 T4432 T4433 compound isotype,switching
R3423 T4433 T4429 conj switching,switching
R3424 T4434 T4423 punct ),process
R3425 T4435 T4436 punct ",",allows
R3426 T4436 T4436 ROOT allows,allows
R3427 T4437 T4438 compound B,cells
R3428 T4438 T4436 dobj cells,allows
R3429 T4439 T4436 punct ",",allows
R3430 T4440 T4441 advmod initially,expressing
R3431 T4441 T4436 advcl expressing,allows
R3432 T4442 T4441 dobj IgM,expressing
R3433 T4443 T4436 punct ",",allows
R3434 T4444 T4445 aux to,switch
R3435 T4445 T4436 advcl switch,allows
R3436 T4446 T4445 prep to,switch
R3437 T4447 T4446 pobj IgA,to
R3438 T4448 T4447 punct ",",IgA
R3439 T4449 T4447 conj IgG,IgA
R3440 T4450 T4449 punct ",",IgG
R3441 T4451 T4449 cc or,IgG
R3442 T4452 T4449 conj IgE,IgG
R3443 T4453 T4445 prep depending,switch
R3444 T4454 T4453 prep on,depending
R3445 T4455 T4456 det the,class
R3446 T4456 T4454 pobj class,on
R3447 T4457 T4456 prep of,class
R3448 T4458 T4457 pobj antibody,of
R3449 T4459 T4460 advmod most,appropriate
R3450 T4460 T4456 amod appropriate,class
R3451 T4461 T4460 prep for,appropriate
R3452 T4462 T4465 det a,response
R3453 T4463 T4465 amod particular,response
R3454 T4464 T4465 amod immune,response
R3455 T4465 T4461 pobj response,for
R3456 T4466 T4436 punct .,allows
R3457 T4467 T4472 nsubjpass Switching,induced
R3458 T4468 T4467 prep to,Switching
R3459 T4469 T4468 pobj IgE,to
R3460 T4470 T4472 aux can,induced
R3461 T4471 T4472 auxpass be,induced
R3462 T4472 T4472 ROOT induced,induced
R3463 T4473 T4472 agent by,induced
R3464 T4474 T4475 det the,combination
R3465 T4475 T4473 pobj combination,by
R3466 T4476 T4475 prep of,combination
R3467 T4477 T4480 nmod IL-4,signals
R3468 T4478 T4477 cc and,IL-4
R3469 T4479 T4480 amod CD40,signals
R3470 T4480 T4476 pobj signals,of
R3471 T4481 T4472 punct .,induced
R3472 T4482 T4484 det The,rearrangement
R3473 T4483 T4484 compound gene,rearrangement
R3474 T4484 T4490 nsubjpass rearrangement,preceded
R3475 T4485 T4484 amod necessary,rearrangement
R3476 T4486 T4485 prep for,necessary
R3477 T4487 T4488 amod IgE,production
R3478 T4488 T4486 pobj production,for
R3479 T4489 T4490 auxpass is,preceded
R3480 T4490 T4490 ROOT preceded,preceded
R3481 T4491 T4490 agent by,preceded
R3482 T4492 T4493 det the,production
R3483 T4493 T4491 pobj production,by
R3484 T4494 T4493 prep of,production
R3485 T4495 T4497 amod non-coding,transcripts
R3486 T4496 T4497 compound RNA,transcripts
R3487 T4497 T4494 pobj transcripts,of
R3488 T4498 T4497 prep from,transcripts
R3489 T4499 T4500 amod germline,sequences
R3490 T4500 T4498 pobj sequences,from
R3491 T4501 T4500 prep in,sequences
R3492 T4502 T4505 det the,chain
R3493 T4503 T4505 nmod IgE,chain
R3494 T4504 T4505 amod heavy,chain
R3495 T4505 T4501 pobj chain,in
R3496 T4506 T4491 pobj locus,by
R3497 T4507 T4509 nmod [,]
R3498 T4508 T4509 nummod 13,]
R3499 T4509 T4506 appos ],locus
R3500 T4510 T4490 punct .,preceded
R3501 T4511 T4512 aux To,determine
R3502 T4512 T4532 advcl determine,stimulated
R3503 T4513 T4516 mark whether,required
R3504 T4514 T4516 nsubjpass HOIP,required
R3505 T4515 T4516 auxpass is,required
R3506 T4516 T4512 ccomp required,determine
R3507 T4517 T4516 prep in,required
R3508 T4518 T4519 det the,initiation
R3509 T4519 T4517 pobj initiation,in
R3510 T4520 T4519 prep of,initiation
R3511 T4521 T4524 det the,process
R3512 T4522 T4524 compound class,process
R3513 T4523 T4524 compound switching,process
R3514 T4524 T4520 pobj process,of
R3515 T4525 T4532 punct ",",stimulated
R3516 T4526 T4530 det the,lines
R3517 T4527 T4530 amod relevant,lines
R3518 T4528 T4529 compound B,cell
R3519 T4529 T4530 compound cell,lines
R3520 T4530 T4532 nsubjpass lines,stimulated
R3521 T4531 T4532 auxpass were,stimulated
R3522 T4532 T4532 ROOT stimulated,stimulated
R3523 T4533 T4532 prep through,stimulated
R3524 T4534 T4533 pobj CD40,through
R3525 T4535 T4532 prep in,stimulated
R3526 T4536 T4537 det the,presence
R3527 T4537 T4535 pobj presence,in
R3528 T4538 T4537 prep of,presence
R3529 T4539 T4538 pobj IL-4,of
R3530 T4540 T4532 cc and,stimulated
R3531 T4541 T4532 conj tested,stimulated
R3532 T4542 T4541 prep for,tested
R3533 T4543 T4542 pobj production,for
R3534 T4544 T4543 prep of,production
R3535 T4545 T4546 compound germline,epsilon
R3536 T4546 T4544 pobj epsilon,of
R3537 T4547 T4548 punct (,GLε
R3538 T4548 T4546 appos GLε,epsilon
R3539 T4549 T4546 punct ),epsilon
R3540 T4550 T4542 pobj transcripts,for
R3541 T4551 T4552 punct (,Fig.
R3542 T4552 T4550 appos Fig.,transcripts
R3543 T4553 T4552 nummod 3,Fig.
R3544 T4554 T4552 punct ),Fig.
R3545 T4555 T4532 punct .,stimulated
R3546 T4556 T4558 amod Anti-CD40,stimulation
R3547 T4557 T4558 compound antibody,stimulation
R3548 T4558 T4564 nsubj stimulation,induced
R3549 T4559 T4558 advmod alone,stimulation
R3550 T4560 T4559 cc or,alone
R3551 T4561 T4559 conj together,alone
R3552 T4562 T4558 prep with,stimulation
R3553 T4563 T4562 pobj IL-4,with
R3554 T4564 T4564 ROOT induced,induced
R3555 T4565 T4566 compound GLε,transcription
R3556 T4566 T4564 dobj transcription,induced
R3557 T4567 T4566 prep in,transcription
R3558 T4568 T4572 amod parental,cells
R3559 T4569 T4572 compound A20,cells
R3560 T4570 T4571 compound .2,J
R3561 T4571 T4572 compound J,cells
R3562 T4572 T4567 pobj cells,in
R3563 T4573 T4574 mark as,expected
R3564 T4574 T4572 advcl expected,cells
R3565 T4575 T4564 punct .,induced
R3566 T4576 T4586 prep In,detected
R3567 T4577 T4576 pobj contrast,In
R3568 T4578 T4586 punct ",",detected
R3569 T4579 T4581 advmod only,levels
R3570 T4580 T4581 amod low,levels
R3571 T4581 T4586 nsubjpass levels,detected
R3572 T4582 T4581 prep of,levels
R3573 T4583 T4584 compound GLε,expression
R3574 T4584 T4582 pobj expression,of
R3575 T4585 T4586 auxpass were,detected
R3576 T4586 T4586 ROOT detected,detected
R3577 T4587 T4586 prep in,detected
R3578 T4588 T4589 amod HOIP-deficient,cells
R3579 T4589 T4587 pobj cells,in
R3580 T4590 T4586 conj stimulated,detected
R3581 T4591 T4590 prep with,stimulated
R3582 T4592 T4594 det either,antibody
R3583 T4593 T4594 amod anti-CD40,antibody
R3584 T4594 T4591 pobj antibody,with
R3585 T4595 T4594 advmod alone,antibody
R3586 T4596 T4594 cc or,antibody
R3587 T4597 T4594 conj anti-CD40,antibody
R3588 T4598 T4597 cc and,anti-CD40
R3589 T4599 T4597 conj IL-4,anti-CD40
R3590 T4600 T4586 punct .,detected
R3591 T4601 T4602 amod Normal,levels
R3592 T4602 T4607 nsubjpass levels,induced
R3593 T4603 T4602 prep of,levels
R3594 T4604 T4605 compound GLε,expression
R3595 T4605 T4603 pobj expression,of
R3596 T4606 T4607 auxpass were,induced
R3597 T4607 T4607 ROOT induced,induced
R3598 T4608 T4607 prep in,induced
R3599 T4609 T4610 amod HOIP-reconstituted,cells
R3600 T4610 T4608 pobj cells,in
R3601 T4611 T4607 conj stimulated,induced
R3602 T4612 T4611 prep with,stimulated
R3603 T4613 T4614 amod anti-CD40,antibody
R3604 T4614 T4612 pobj antibody,with
R3605 T4615 T4614 advmod alone,antibody
R3606 T4616 T4615 cc or,alone
R3607 T4617 T4618 advmod together,with
R3608 T4618 T4615 conj with,alone
R3609 T4619 T4618 pobj IL-4,with
R3610 T4620 T4607 punct .,induced
R3611 T4621 T4625 advmod Collectively,demonstrate
R3612 T4622 T4625 punct ",",demonstrate
R3613 T4623 T4624 det these,data
R3614 T4624 T4625 nsubj data,demonstrate
R3615 T4625 T4625 ROOT demonstrate,demonstrate
R3616 T4626 T4628 mark that,has
R3617 T4627 T4628 nsubj HOIP,has
R3618 T4628 T4625 ccomp has,demonstrate
R3619 T4629 T4631 det a,role
R3620 T4630 T4631 amod critical,role
R3621 T4631 T4628 dobj role,has
R3622 T4632 T4631 prep in,role
R3623 T4633 T4634 amod cellular,responses
R3624 T4634 T4632 pobj responses,in
R3625 T4635 T4634 prep to,responses
R3626 T4636 T4637 dep CD40,signaling
R3627 T4637 T4628 prep signaling,has
R3628 T4638 T4625 punct .,demonstrate
R4202 T5508 T5509 nsubj HOIP,mediates
R4203 T5509 T5509 ROOT mediates,mediates
R4204 T5510 T5511 amod CD40-stimulated,NF-κB
R4205 T5511 T5509 dobj NF-κB,mediates
R4206 T5512 T5511 cc and,NF-κB
R4207 T5513 T5514 compound JNK,activation
R4208 T5514 T5511 conj activation,NF-κB
R4209 T5515 T5516 det The,results
R4210 T5516 T5509 dobj results,mediates
R4211 T5517 T5516 acl described,results
R4212 T5518 T5517 prep above,described
R4213 T5519 T5518 pobj show,above
R4214 T5520 T5522 mark that,plays
R4215 T5521 T5522 nsubj HOIP,plays
R4216 T5522 T5519 ccomp plays,show
R4217 T5523 T5525 det an,role
R4218 T5524 T5525 amod important,role
R4219 T5525 T5522 dobj role,plays
R4220 T5526 T5525 prep in,role
R4221 T5527 T5529 amod CD40-mediated,functions
R4222 T5528 T5529 compound effector,functions
R4223 T5529 T5526 pobj functions,in
R4224 T5530 T5529 prep of,functions
R4225 T5531 T5532 compound B,cells
R4226 T5532 T5530 pobj cells,of
R4227 T5533 T5509 punct .,mediates
R4228 T5534 T5535 nsubj It,follows
R4229 T5535 T5535 ROOT follows,follows
R4230 T5536 T5538 mark that,is
R4231 T5537 T5538 nsubj HOIP,is
R4232 T5538 T5535 ccomp is,follows
R4233 T5539 T5538 acomp likely,is
R4234 T5540 T5542 det a,mediator
R4235 T5541 T5542 amod key,mediator
R4236 T5542 T5539 pobj mediator,likely
R4237 T5543 T5542 prep of,mediator
R4238 T5544 T5543 pobj CD40,of
R4239 T5545 T5542 acl signaling,mediator
R4240 T5546 T5535 punct .,follows
R4241 T5547 T5548 aux To,test
R4242 T5548 T5553 advcl test,stimulated
R4243 T5549 T5550 det this,hypothesis
R4244 T5550 T5548 dobj hypothesis,test
R4245 T5551 T5553 punct ",",stimulated
R4246 T5552 T5553 nsubj we,stimulated
R4247 T5553 T5553 ROOT stimulated,stimulated
R4248 T5554 T5556 nmod A20,J
R4249 T5555 T5556 nummod .2,J
R4250 T5556 T5553 dobj J,stimulated
R4251 T5557 T5556 cc and,J
R4252 T5558 T5559 amod HOIP-deficient,cells
R4253 T5559 T5556 conj cells,J
R4254 T5560 T5553 prep with,stimulated
R4255 T5561 T5560 pobj CD154,with
R4256 T5562 T5561 punct (,CD154
R4257 T5563 T5564 compound CD40,ligand
R4258 T5564 T5561 appos ligand,CD154
R4259 T5565 T5561 punct ),CD154
R4260 T5566 T5553 advcl expressed,stimulated
R4261 T5567 T5566 agent by,expressed
R4262 T5568 T5570 nummod HI5,cells
R4263 T5569 T5570 compound insect,cells
R4264 T5570 T5567 pobj cells,by
R4265 T5571 T5573 nmod [,]
R4266 T5572 T5573 nummod 7,]
R4267 T5573 T5553 npadvmod ],stimulated
R4268 T5574 T5573 punct ",",]
R4269 T5575 T5577 compound [,]
R4270 T5576 T5577 nummod 8,]
R4271 T5577 T5573 conj ],]
R4272 T5578 T5577 cc and,]
R4273 T5579 T5580 amod measured,activation
R4274 T5580 T5577 conj activation,]
R4275 T5581 T5580 prep of,activation
R4276 T5582 T5583 det the,NF-κB
R4277 T5583 T5581 pobj NF-κB,of
R4278 T5584 T5583 cc and,NF-κB
R4279 T5585 T5583 conj JNK,NF-κB
R4280 T5586 T5580 conj pathways,activation
R4281 T5587 T5586 punct ",",pathways
R4282 T5588 T5586 appos two,pathways
R4283 T5589 T5588 prep of,two
R4284 T5590 T5593 det the,regulators
R4285 T5591 T5593 amod major,regulators
R4286 T5592 T5593 amod transcriptional,regulators
R4287 T5593 T5589 pobj regulators,of
R4288 T5594 T5588 acl activated,two
R4289 T5595 T5594 agent by,activated
R4290 T5596 T5597 compound CD40,[
R4291 T5597 T5595 pobj [,by
R4292 T5598 T5599 nummod 4,]
R4293 T5599 T5597 appos ],[
R4294 T5600 T5553 punct .,stimulated
R4295 T5601 T5602 compound Cell-associated,CD154
R4296 T5602 T5604 nsubjpass CD154,used
R4297 T5603 T5604 auxpass was,used
R4298 T5604 T5604 ROOT used,used
R4299 T5605 T5604 prep as,used
R4300 T5606 T5607 det the,stimulus
R4301 T5607 T5605 pobj stimulus,as
R4302 T5608 T5607 prep in,stimulus
R4303 T5609 T5610 det these,experiments
R4304 T5610 T5608 pobj experiments,in
R4305 T5611 T5614 mark as,provides
R4306 T5612 T5614 nsubj it,provides
R4307 T5613 T5614 advmod typically,provides
R4308 T5614 T5604 advcl provides,used
R4309 T5615 T5616 advmod more,robust
R4310 T5616 T5614 advmod robust,provides
R4311 T5617 T5614 punct ",",provides
R4312 T5618 T5614 cc and,provides
R4313 T5619 T5622 advmod therefore,detected
R4314 T5620 T5621 advmod more,readily
R4315 T5621 T5622 advmod readily,detected
R4316 T5622 T5625 amod detected,signals
R4317 T5623 T5625 punct ",",signals
R4318 T5624 T5625 compound activation,signals
R4319 T5625 T5614 conj signals,provides
R4320 T5626 T5627 mark than,does
R4321 T5627 T5625 advcl does,signals
R4322 T5628 T5629 amod anti-CD40,antibody
R4323 T5629 T5627 nsubj antibody,does
R4324 T5630 T5604 punct .,used
R4325 T5631 T5638 nsubjpass Activation,initiated
R4326 T5632 T5631 prep of,Activation
R4327 T5633 T5636 det the,pathway
R4328 T5634 T5636 amod canonical,pathway
R4329 T5635 T5636 compound NF-κB,pathway
R4330 T5636 T5632 pobj pathway,of
R4331 T5637 T5638 auxpass is,initiated
R4332 T5638 T5638 ROOT initiated,initiated
R4333 T5639 T5638 prep with,initiated
R4334 T5640 T5641 det the,phosphorylation
R4335 T5641 T5639 pobj phosphorylation,with
R4336 T5642 T5641 prep of,phosphorylation
R4337 T5643 T5644 compound IκB,proteins
R4338 T5644 T5642 pobj proteins,of
R4339 T5645 T5641 prep by,phosphorylation
R4340 T5646 T5649 det the,complex
R4341 T5647 T5649 nmod IκB,complex
R4342 T5648 T5649 compound kinase,complex
R4343 T5649 T5645 pobj complex,by
R4344 T5650 T5651 punct (,IKK
R4345 T5651 T5649 appos IKK,complex
R4346 T5652 T5651 punct ),IKK
R4347 T5653 T5638 punct .,initiated
R4348 T5654 T5660 prep In,are
R4349 T5655 T5656 amod resting,cells
R4350 T5656 T5654 pobj cells,In
R4351 T5657 T5660 punct ",",are
R4352 T5658 T5659 compound IκB,proteins
R4353 T5659 T5660 nsubj proteins,are
R4354 T5660 T5660 ROOT are,are
R4355 T5661 T5660 acomp responsible,are
R4356 T5662 T5661 prep for,responsible
R4357 T5663 T5665 compound sequestering,subunits
R4358 T5664 T5665 compound NF-κB,subunits
R4359 T5665 T5662 pobj subunits,for
R4360 T5666 T5665 prep in,subunits
R4361 T5667 T5668 det the,cytoplasm
R4362 T5668 T5666 pobj cytoplasm,in
R4363 T5669 T5660 punct .,are
R4364 T5670 T5670 ROOT Phosphorylation,Phosphorylation
R4365 T5671 T5670 prep by,Phosphorylation
R4366 T5672 T5675 det the,targets
R4367 T5673 T5675 nmod IKK,targets
R4368 T5674 T5675 amod complex,targets
R4369 T5675 T5671 pobj targets,by
R4370 T5676 T5677 amod IκB,proteins
R4371 T5677 T5670 conj proteins,Phosphorylation
R4372 T5678 T5677 prep for,proteins
R4373 T5679 T5678 pobj ubiquitination,for
R4374 T5680 T5679 cc and,ubiquitination
R4375 T5681 T5679 conj degradation,ubiquitination
R4376 T5682 T5677 punct ",",proteins
R4377 T5683 T5670 acl allowing,Phosphorylation
R4378 T5684 T5686 nsubj NF-κB,enter
R4379 T5685 T5686 aux to,enter
R4380 T5686 T5683 ccomp enter,allowing
R4381 T5687 T5688 det the,nucleus
R4382 T5688 T5686 dobj nucleus,enter
R4383 T5689 T5686 cc and,enter
R4384 T5690 T5686 conj activate,enter
R4385 T5691 T5692 compound gene,expression
R4386 T5692 T5690 dobj expression,activate
R4387 T5693 T5670 punct .,Phosphorylation
R4388 T5694 T5695 amod CD40-mediated,phosphorylation
R4389 T5695 T5705 nsubjpass phosphorylation,impaired
R4390 T5696 T5695 cc and,phosphorylation
R4391 T5697 T5695 conj degradation,phosphorylation
R4392 T5698 T5697 prep of,degradation
R4393 T5699 T5698 pobj IκBα,of
R4394 T5700 T5697 prep in,degradation
R4395 T5701 T5702 amod HOIP-deficient,cells
R4396 T5702 T5700 pobj cells,in
R4397 T5703 T5705 auxpass was,impaired
R4398 T5704 T5705 advmod dramatically,impaired
R4399 T5705 T5705 ROOT impaired,impaired
R4400 T5706 T5705 oprd relative,impaired
R4401 T5707 T5706 prep to,relative
R4402 T5708 T5709 nsubj that,observed
R4403 T5709 T5707 pobj observed,to
R4404 T5710 T5709 prep in,observed
R4405 T5711 T5715 amod parental,cells
R4406 T5712 T5715 compound A20,cells
R4407 T5713 T5714 compound .2,J
R4408 T5714 T5715 compound J,cells
R4409 T5715 T5710 pobj cells,in
R4410 T5716 T5717 punct (,Fig.
R4411 T5717 T5719 nmod Fig.,)
R4412 T5718 T5717 nummod 4,Fig.
R4413 T5719 T5705 punct ),impaired
R4414 T5720 T5705 punct .,impaired
R4415 T5721 T5723 nsubj We,assayed
R4416 T5722 T5723 advmod also,assayed
R4417 T5723 T5723 ROOT assayed,assayed
R4418 T5724 T5723 dobj activation,assayed
R4419 T5725 T5724 prep of,activation
R4420 T5726 T5728 det the,protein
R4421 T5727 T5728 amod stress-activated,protein
R4422 T5728 T5725 pobj protein,of
R4423 T5729 T5730 compound kinase,JNK
R4424 T5730 T5728 appos JNK,protein
R4425 T5731 T5723 prep in,assayed
R4426 T5732 T5731 pobj response,in
R4427 T5733 T5732 prep to,response
R4428 T5734 T5735 nummod CD40,engagement
R4429 T5735 T5733 pobj engagement,to
R4430 T5736 T5738 punct (,4
R4431 T5737 T5738 nmod Fig.,4
R4432 T5738 T5735 appos 4,engagement
R4433 T5739 T5738 punct ),4
R4434 T5740 T5723 punct .,assayed
R4435 T5741 T5743 amod CD40-mediated,activation
R4436 T5742 T5743 compound JNK,activation
R4437 T5743 T5748 nsubjpass activation,impaired
R4438 T5744 T5743 prep in,activation
R4439 T5745 T5746 amod HOIP-deficient,cells
R4440 T5746 T5744 pobj cells,in
R4441 T5747 T5748 auxpass was,impaired
R4442 T5748 T5748 ROOT impaired,impaired
R4443 T5749 T5750 mark as,measured
R4444 T5750 T5748 advcl measured,impaired
R4445 T5751 T5750 agent by,measured
R4446 T5752 T5751 pobj phosphorylation,by
R4447 T5753 T5752 prep of,phosphorylation
R4448 T5754 T5753 pobj Thr183,of
R4449 T5755 T5754 cc and,Thr183
R4450 T5756 T5754 conj Tyr185,Thr183
R4451 T5757 T5752 prep in,phosphorylation
R4452 T5758 T5757 pobj JNK,in
R4453 T5759 T5748 punct .,impaired
R4454 T5760 T5761 amod CD40-induced,activation
R4455 T5761 T5769 nsubj activation,was
R4456 T5762 T5761 prep of,activation
R4457 T5763 T5762 pobj NF-κB,of
R4458 T5764 T5763 cc and,NF-κB
R4459 T5765 T5763 conj JNK,NF-κB
R4460 T5766 T5761 prep in,activation
R4461 T5767 T5768 amod HOIP-reconstituted,cells
R4462 T5768 T5766 pobj cells,in
R4463 T5769 T5769 ROOT was,was
R4464 T5770 T5769 acomp normal,was
R4465 T5771 T5769 punct ",",was
R4466 T5772 T5769 advcl demonstrating,was
R4467 T5773 T5776 mark that,observed
R4468 T5774 T5775 det the,defects
R4469 T5775 T5776 nsubj defects,observed
R4470 T5776 T5772 ccomp observed,demonstrating
R4471 T5777 T5776 prep in,observed
R4472 T5778 T5779 amod gene-deficient,cells
R4473 T5779 T5777 pobj cells,in
R4474 T5780 T5769 conj were,was
R4475 T5781 T5780 acomp due,were
R4476 T5782 T5781 prep to,due
R4477 T5783 T5784 det the,absence
R4478 T5784 T5782 pobj absence,to
R4479 T5785 T5784 prep of,absence
R4480 T5786 T5787 compound HOIP,expression
R4481 T5787 T5785 pobj expression,of
R4482 T5788 T5769 punct .,was
R5098 T6740 T6739 pobj CD40,with
R5099 T6741 T6742 nsubj The,marked
R5101 T6743 T6742 dobj defects,marked
R5102 T6744 T6743 prep in,defects
R5103 T6745 T6747 amod CD40-mediated,activation
R5104 T6746 T6747 compound cell,activation
R5105 T6747 T6744 pobj activation,in
R5106 T6748 T6742 cc and,marked
R5107 T6749 T6754 nsubj signaling,suggested
R5108 T6750 T6749 acl displayed,signaling
R5109 T6751 T6750 agent by,displayed
R5110 T6752 T6753 amod HOIP-deficient,cells
R5111 T6753 T6751 pobj cells,by
R5112 T6754 T6742 conj suggested,marked
R5113 T6755 T6757 mark that,mediates
R5114 T6756 T6757 nsubj HOIP,mediates
R5115 T6757 T6754 ccomp mediates,suggested
R5116 T6758 T6757 dobj recruitment,mediates
R5117 T6759 T6758 prep of,recruitment
R5118 T6760 T6761 amod critical,components
R5119 T6761 T6759 pobj components,of
R5120 T6762 T6761 prep of,components
R5121 T6763 T6766 det the,apparatus
R5122 T6764 T6766 nmod CD40,apparatus
R5123 T6765 T6766 amod signaling,apparatus
R5124 T6766 T6762 pobj apparatus,of
R5125 T6767 T6758 prep to,recruitment
R5126 T6768 T6769 det the,receptor
R5127 T6769 T6767 pobj receptor,to
R5128 T6770 T6754 punct .,suggested
R5129 T6771 T6774 advmod Previously,demonstrated
R5130 T6772 T6774 punct ",",demonstrated
R5131 T6773 T6774 nsubj we,demonstrated
R5132 T6774 T6774 ROOT demonstrated,demonstrated
R5133 T6775 T6778 mark that,recruited
R5134 T6776 T6778 nsubjpass HOIP,recruited
R5135 T6777 T6778 auxpass is,recruited
R5136 T6778 T6774 ccomp recruited,demonstrated
R5137 T6779 T6778 prep to,recruited
R5138 T6780 T6781 det the,CD40
R5139 T6781 T6782 nsubj CD40,signaling
R5140 T6782 T6779 pcomp signaling,to
R5141 T6783 T6782 dobj complex,signaling
R5142 T6784 T6782 prep in,signaling
R5143 T6785 T6787 det a,manner
R5144 T6786 T6787 amod TRAF2-dependent,manner
R5145 T6787 T6784 pobj manner,in
R5146 T6788 T6774 punct ",",demonstrated
R5147 T6789 T6774 advcl suggesting,demonstrated
R5148 T6790 T6792 mark that,functions
R5149 T6791 T6792 compound HOIP,functions
R5150 T6792 T6789 ccomp functions,suggesting
R5151 T6793 T6792 amod downstream,functions
R5152 T6794 T6793 prep of,downstream
R5153 T6795 T6798 nummod TRAF2,]
R5154 T6796 T6798 nmod [,]
R5155 T6797 T6796 nummod 6,[
R5156 T6798 T6794 pobj ],of
R5157 T6799 T6774 punct .,demonstrated
R5158 T6800 T6803 nsubj Studies,suggest
R5159 T6801 T6800 prep by,Studies
R5160 T6802 T6801 pobj others,by
R5161 T6803 T6803 ROOT suggest,suggest
R5162 T6804 T6808 mark that,cIAP1
R5163 T6805 T6807 det the,proteins
R5164 T6806 T6807 amod TRAF2-associated,proteins
R5165 T6807 T6808 nsubj proteins,cIAP1
R5166 T6808 T6803 ccomp cIAP1,suggest
R5167 T6809 T6808 cc and,cIAP1
R5168 T6810 T6808 conj cIAP2,cIAP1
R5169 T6811 T6803 conj play,suggest
R5170 T6812 T6813 det a,role
R5171 T6813 T6811 dobj role,play
R5172 T6814 T6813 prep in,role
R5173 T6815 T6816 det the,recruitment
R5174 T6816 T6814 pobj recruitment,in
R5175 T6817 T6816 prep of,recruitment
R5176 T6818 T6817 pobj HOIP,of
R5177 T6819 T6813 prep to,role
R5178 T6820 T6819 pobj TNFR1,to
R5179 T6821 T6820 cc and,TNFR1
R5180 T6822 T6820 conj CD40,TNFR1
R5181 T6823 T6825 nmod [,]
R5182 T6824 T6825 nummod 14,]
R5183 T6825 T6819 pobj ],to
R5184 T6826 T6803 punct .,suggest
R5185 T6827 T6830 advmod Therefore,determined
R5186 T6828 T6830 punct ",",determined
R5187 T6829 T6830 nsubj we,determined
R5188 T6830 T6830 ROOT determined,determined
R5189 T6831 T6844 mark whether,altered
R5190 T6832 T6833 det the,association
R5191 T6833 T6844 nsubjpass association,altered
R5192 T6834 T6833 prep of,association
R5193 T6835 T6834 pobj HOIP,of
R5194 T6836 T6833 prep with,association
R5195 T6837 T6836 pobj CD40,with
R5196 T6838 T6833 prep in,association
R5197 T6839 T6838 pobj A20,in
R5198 T6840 T6841 compound .2,J
R5199 T6841 T6842 compound J,cells
R5200 T6842 T6844 nsubjpass cells,altered
R5201 T6843 T6844 auxpass was,altered
R5202 T6844 T6830 ccomp altered,determined
R5203 T6845 T6844 agent by,altered
R5204 T6846 T6845 pobj treatment,by
R5205 T6847 T6846 prep of,treatment
R5206 T6848 T6847 pobj cells,of
R5207 T6849 T6844 prep with,altered
R5208 T6850 T6851 det an,inhibitor
R5209 T6851 T6849 pobj inhibitor,with
R5210 T6852 T6851 prep of,inhibitor
R5211 T6853 T6854 amod cIAP,activity
R5212 T6854 T6852 pobj activity,of
R5213 T6855 T6851 punct ",",inhibitor
R5214 T6856 T6858 det a,peptide
R5215 T6857 T6858 amod membrane-permeable,peptide
R5216 T6858 T6851 appos peptide,inhibitor
R5217 T6859 T6858 acl derived,peptide
R5218 T6860 T6859 prep from,derived
R5219 T6861 T6863 det the,regulator
R5220 T6862 T6863 compound apoptosis,regulator
R5221 T6863 T6860 pobj regulator,from
R5222 T6864 T6865 compound SMAC,[
R5223 T6865 T6863 appos [,regulator
R5224 T6866 T6867 nummod 15,]
R5225 T6867 T6863 appos ],regulator
R5226 T6868 T6830 punct .,determined
R5227 T6869 T6870 nsubj We,found
R5228 T6870 T6870 ROOT found,found
R5229 T6871 T6880 mark that,reduced
R5230 T6872 T6880 nsubj pretreatment,reduced
R5231 T6873 T6872 prep of,pretreatment
R5232 T6874 T6873 pobj cells,of
R5233 T6875 T6872 prep with,pretreatment
R5234 T6876 T6878 det the,peptide
R5235 T6877 T6878 compound SMAC,peptide
R5236 T6878 T6875 pobj peptide,with
R5237 T6879 T6880 advmod dramatically,reduced
R5238 T6880 T6870 ccomp reduced,found
R5239 T6881 T6882 det the,amount
R5240 T6882 T6880 dobj amount,reduced
R5241 T6883 T6882 prep of,amount
R5242 T6884 T6883 pobj cIAP1,of
R5243 T6885 T6882 acl associated,amount
R5244 T6886 T6885 prep with,associated
R5245 T6887 T6888 det the,CD40
R5246 T6888 T6889 nsubj CD40,signaling
R5247 T6889 T6886 pcomp signaling,with
R5248 T6890 T6889 ccomp complex,signaling
R5249 T6891 T6890 prep in,complex
R5250 T6892 T6891 pobj cells,in
R5251 T6893 T6892 acl stimulated,cells
R5252 T6894 T6893 prep with,stimulated
R5253 T6895 T6897 amod anti-CD40,beads
R5254 T6896 T6897 amod antibody-coated,beads
R5255 T6897 T6894 pobj beads,with
R5256 T6898 T6897 punct (,beads
R5257 T6899 T6900 compound Fig.,5A
R5258 T6900 T6897 appos 5A,beads
R5259 T6901 T6897 punct ),beads
R5260 T6902 T6870 punct .,found
R5261 T6903 T6905 compound SMAC,treatment
R5262 T6904 T6905 compound peptide,treatment
R5263 T6905 T6907 nsubj treatment,resulted
R5264 T6906 T6907 advmod also,resulted
R5265 T6907 T6907 ROOT resulted,resulted
R5266 T6908 T6907 prep in,resulted
R5267 T6909 T6913 det a,decrease
R5268 T6910 T6913 amod slight,decrease
R5269 T6911 T6910 cc but,slight
R5270 T6912 T6910 conj reproducible,slight
R5271 T6913 T6908 pobj decrease,in
R5272 T6914 T6913 prep in,decrease
R5273 T6915 T6916 det the,amount
R5274 T6916 T6914 pobj amount,in
R5275 T6917 T6916 prep of,amount
R5276 T6918 T6921 det the,form
R5277 T6919 T6921 amod major,form
R5278 T6920 T6921 compound HOIP,form
R5279 T6921 T6917 pobj form,of
R5280 T6922 T6921 acl recovered,form
R5281 T6923 T6922 agent by,recovered
R5282 T6924 T6925 compound CD40,immunoprecipitation
R5283 T6925 T6923 pobj immunoprecipitation,by
R5284 T6926 T6907 punct ",",resulted
R5285 T6927 T6907 prep along,resulted
R5286 T6928 T6927 prep with,along
R5287 T6929 T6931 det an,increase
R5288 T6930 T6931 amod apparent,increase
R5289 T6931 T6928 pobj increase,with
R5290 T6932 T6931 prep in,increase
R5291 T6933 T6936 amod higher,species
R5292 T6934 T6936 amod molecular,species
R5293 T6935 T6936 compound weight,species
R5294 T6936 T6932 pobj species,in
R5295 T6937 T6936 acl recognized,species
R5296 T6938 T6937 agent by,recognized
R5297 T6939 T6940 amod anti-HOIP,antibody
R5298 T6940 T6938 pobj antibody,by
R5299 T6941 T6907 punct .,resulted
R5300 T6942 T6952 prep In,alter
R5301 T6943 T6942 pobj contrast,In
R5302 T6944 T6952 punct ",",alter
R5303 T6945 T6952 nsubj treatment,alter
R5304 T6946 T6945 prep with,treatment
R5305 T6947 T6949 det the,peptide
R5306 T6948 T6949 compound SMAC,peptide
R5307 T6949 T6946 pobj peptide,with
R5308 T6950 T6952 aux did,alter
R5309 T6951 T6952 neg not,alter
R5310 T6952 T6952 ROOT alter,alter
R5311 T6953 T6954 det the,amount
R5312 T6954 T6952 dobj amount,alter
R5313 T6955 T6954 cc or,amount
R5314 T6956 T6957 amod molecular,weight
R5315 T6957 T6954 conj weight,amount
R5316 T6958 T6954 prep of,amount
R5317 T6959 T6960 compound HOIP,present
R5318 T6960 T6958 pobj present,of
R5319 T6961 T6960 prep in,present
R5320 T6962 T6963 compound cell,lysates
R5321 T6963 T6961 pobj lysates,in
R5322 T6964 T6952 punct .,alter
R5323 T6965 T6966 det These,data
R5324 T6966 T6967 nsubj data,suggest
R5325 T6967 T6967 ROOT suggest,suggest
R5326 T6968 T6973 mark that,alters
R5327 T6969 T6971 compound SMAC,treatment
R5328 T6970 T6971 compound peptide,treatment
R5329 T6971 T6973 nsubj treatment,alters
R5330 T6972 T6973 advmod specifically,alters
R5331 T6973 T6967 ccomp alters,suggest
R5332 T6974 T6975 det the,characteristics
R5333 T6975 T6973 dobj characteristics,alters
R5334 T6976 T6975 prep of,characteristics
R5335 T6977 T6978 compound CD40-associated,HOIP
R5336 T6978 T6976 pobj HOIP,of
R5337 T6979 T6980 advmod rather,than
R5338 T6980 T6975 cc than,characteristics
R5339 T6981 T6984 det the,pool
R5340 T6982 T6984 amod entire,pool
R5341 T6983 T6984 amod cellular,pool
R5342 T6984 T6980 pobj pool,than
R5343 T6985 T6984 prep of,pool
R5344 T6986 T6987 det this,protein
R5345 T6987 T6985 pobj protein,of
R5346 T6988 T6967 punct .,suggest
R5347 T6989 T6993 advmod Together,support
R5348 T6990 T6993 punct ",",support
R5349 T6991 T6992 det these,results
R5350 T6992 T6993 nsubj results,support
R5351 T6993 T6993 ROOT support,support
R5352 T6994 T6995 det the,idea
R5353 T6995 T6993 dobj idea,support
R5354 T6996 T7000 mark that,influence
R5355 T6997 T6999 det the,proteins
R5356 T6998 T6999 compound cIAP,proteins
R5357 T6999 T7000 nsubj proteins,influence
R5358 T7000 T6995 acl influence,idea
R5359 T7001 T7002 det the,recruitment
R5360 T7002 T7000 dobj recruitment,influence
R5361 T7003 T7002 cc and,recruitment
R5362 T7004 T7006 amod post-translational,state
R5363 T7005 T7006 compound modification,state
R5364 T7006 T7002 conj state,recruitment
R5365 T7007 T7002 prep of,recruitment
R5366 T7008 T7009 compound CD40-associated,HOIP
R5367 T7009 T7007 pobj HOIP,of
R5368 T7010 T6993 punct .,support
R5369 T7011 T7012 aux To,test
R5370 T7012 T7031 advcl test,immunoprecipitated
R5371 T7013 T7014 det the,possibility
R5372 T7014 T7012 dobj possibility,test
R5373 T7015 T7017 mark that,is
R5374 T7016 T7017 nsubj HOIP,is
R5375 T7017 T7014 acl is,possibility
R5376 T7018 T7017 acomp responsible,is
R5377 T7019 T7018 prep for,responsible
R5378 T7020 T7021 det the,recruitment
R5379 T7021 T7019 pobj recruitment,for
R5380 T7022 T7021 prep of,recruitment
R5381 T7023 T7026 amod other,proteins
R5382 T7024 T7026 amod critical,proteins
R5383 T7025 T7026 amod signaling,proteins
R5384 T7026 T7022 pobj proteins,of
R5385 T7027 T7021 prep to,recruitment
R5386 T7028 T7027 pobj CD40,to
R5387 T7029 T7031 punct ",",immunoprecipitated
R5388 T7030 T7031 nsubj we,immunoprecipitated
R5389 T7031 T7031 ROOT immunoprecipitated,immunoprecipitated
R5390 T7032 T7031 dobj CD40,immunoprecipitated
R5391 T7033 T7031 advcl signaling,immunoprecipitated
R5392 T7034 T7033 dobj complexes,signaling
R5393 T7035 T7034 prep from,complexes
R5394 T7036 T7039 amod HOIP-deficient,cells
R5395 T7037 T7036 cc and,HOIP-deficient
R5396 T7038 T7036 conj HOIP-reconstituted,HOIP-deficient
R5397 T7039 T7035 pobj cells,from
R5398 T7040 T7034 punct (,complexes
R5399 T7041 T7042 compound Fig.,5B
R5400 T7042 T7034 appos 5B,complexes
R5401 T7043 T7034 punct ),complexes
R5402 T7044 T7031 punct .,immunoprecipitated
R5403 T7045 T7046 mark As,described
R5404 T7046 T7058 advcl described,contained
R5405 T7047 T7046 advmod previously,described
R5406 T7048 T7058 punct ",",contained
R5407 T7049 T7058 nsubj CD40,contained
R5408 T7050 T7058 advcl signaling,contained
R5409 T7051 T7058 nsubj complexes,contained
R5410 T7052 T7051 acl immunoprecipitated,complexes
R5411 T7053 T7052 prep from,immunoprecipitated
R5412 T7054 T7057 nummod A20,cells
R5413 T7055 T7057 nummod .2,cells
R5414 T7056 T7057 compound J,cells
R5415 T7057 T7053 pobj cells,from
R5416 T7058 T7058 ROOT contained,contained
R5417 T7059 T7058 dobj HOIP,contained
R5418 T7060 T7062 advmod as,as
R5419 T7061 T7062 advmod well,as
R5420 T7062 T7059 cc as,HOIP
R5421 T7063 T7059 conj TRAF2,HOIP
R5422 T7064 T7059 punct ",",HOIP
R5423 T7065 T7059 appos TRAF3,HOIP
R5424 T7066 T7059 punct ",",HOIP
R5425 T7067 T7069 compound IKKα,β
R5426 T7068 T7069 compound /,β
R5427 T7069 T7059 appos β,HOIP
R5428 T7070 T7059 punct ",",HOIP
R5429 T7071 T7059 cc and,HOIP
R5430 T7072 T7073 compound IKKγ,[
R5431 T7073 T7059 conj [,HOIP
R5432 T7074 T7075 nummod 6,]
R5433 T7075 T7059 conj ],HOIP
R5434 T7076 T7058 punct .,contained
R5435 T7077 T7078 det The,amounts
R5436 T7078 T7092 nsubj amounts,were
R5437 T7079 T7078 prep of,amounts
R5438 T7080 T7079 pobj TRAF2,of
R5439 T7081 T7078 punct ",",amounts
R5440 T7082 T7078 appos TRAF3,amounts
R5441 T7083 T7078 punct ",",amounts
R5442 T7084 T7078 cc and,amounts
R5443 T7085 T7078 conj cIAP1,amounts
R5444 T7086 T7085 prep in,cIAP1
R5445 T7087 T7086 pobj CD40,in
R5446 T7088 T7085 dep immunoprecipitates,cIAP1
R5447 T7089 T7088 prep from,immunoprecipitates
R5448 T7090 T7091 amod HOIP-deficient,cells
R5449 T7091 T7089 pobj cells,from
R5450 T7092 T7092 ROOT were,were
R5451 T7093 T7092 acomp similar,were
R5452 T7094 T7093 prep to,similar
R5453 T7095 T7094 pobj those,to
R5454 T7096 T7095 prep from,those
R5455 T7097 T7101 amod parental,cells
R5456 T7098 T7101 compound A20,cells
R5457 T7099 T7100 compound .2,J
R5458 T7100 T7101 compound J,cells
R5459 T7101 T7096 pobj cells,from
R5460 T7102 T7092 punct ",",were
R5461 T7103 T7092 advcl indicating,were
R5462 T7104 T7108 mark that,required
R5463 T7105 T7108 nsubjpass HOIP,required
R5464 T7106 T7108 auxpass is,required
R5465 T7107 T7108 neg not,required
R5466 T7108 T7103 ccomp required,indicating
R5467 T7109 T7108 prep for,required
R5468 T7110 T7111 det the,association
R5469 T7111 T7109 pobj association,for
R5470 T7112 T7111 prep of,association
R5471 T7113 T7114 det these,proteins
R5472 T7114 T7112 pobj proteins,of
R5473 T7115 T7111 prep with,association
R5474 T7116 T7115 pobj CD40,with
R5475 T7117 T7092 punct .,were
R5476 T7118 T7126 prep In,were
R5477 T7119 T7118 pobj contrast,In
R5478 T7120 T7126 punct ",",were
R5479 T7121 T7123 compound IKKα,β
R5480 T7122 T7123 compound /,β
R5481 T7123 T7126 nsubj β,were
R5482 T7124 T7123 cc and,β
R5483 T7125 T7123 conj IKKγ,β
R5484 T7126 T7126 ROOT were,were
R5485 T7127 T7126 neg not,were
R5486 T7128 T7126 acomp detectable,were
R5487 T7129 T7128 prep in,detectable
R5488 T7130 T7131 nummod CD40,immunoprecipitates
R5489 T7131 T7129 pobj immunoprecipitates,in
R5490 T7132 T7131 acl recovered,immunoprecipitates
R5491 T7133 T7132 prep from,recovered
R5492 T7134 T7135 amod HOIP-deficient,cells
R5493 T7135 T7133 pobj cells,from
R5494 T7136 T7132 punct (,recovered
R5495 T7137 T7138 compound Fig.,5B
R5496 T7138 T7138 ROOT 5B,5B
R5497 T7139 T7140 punct ",",C
R5498 T7140 T7138 appos C,5B
R5499 T7141 T7138 punct ),5B
R5500 T7142 T7132 punct .,recovered
R5501 T7143 T7144 det The,amounts
R5502 T7144 T7153 nsubj amounts,immunoprecipitates
R5503 T7145 T7144 prep of,amounts
R5504 T7146 T7148 compound IKKα,β
R5505 T7147 T7148 compound /,β
R5506 T7148 T7145 pobj β,of
R5507 T7149 T7148 cc and,β
R5508 T7150 T7148 conj IKKγ,β
R5509 T7151 T7150 prep in,IKKγ
R5510 T7152 T7151 pobj CD40,in
R5511 T7153 T7157 nsubj immunoprecipitates,were
R5512 T7154 T7153 prep from,immunoprecipitates
R5513 T7155 T7156 amod HOIP-reconstituted,cells
R5514 T7156 T7154 pobj cells,from
R5515 T7157 T7157 ROOT were,were
R5516 T7158 T7157 acomp similar,were
R5517 T7159 T7158 prep to,similar
R5518 T7160 T7159 pobj those,to
R5519 T7161 T7160 acl detected,those
R5520 T7162 T7161 prep in,detected
R5521 T7163 T7162 pobj samples,in
R5522 T7164 T7163 acl prepared,samples
R5523 T7165 T7164 prep from,prepared
R5524 T7166 T7167 amod parental,cells
R5525 T7167 T7165 pobj cells,from
R5526 T7168 T7157 punct ",",were
R5527 T7169 T7157 advcl demonstrating,were
R5528 T7170 T7178 mark that,were
R5529 T7171 T7172 det the,defects
R5530 T7172 T7178 nsubj defects,were
R5531 T7173 T7172 prep in,defects
R5532 T7174 T7175 compound IKK,recruitment
R5533 T7175 T7173 pobj recruitment,in
R5534 T7176 T7177 nsubj we,observed
R5535 T7177 T7172 relcl observed,defects
R5536 T7178 T7169 ccomp were,demonstrating
R5537 T7179 T7178 acomp due,were
R5538 T7180 T7181 advmod specifically,to
R5539 T7181 T7179 prep to,due
R5540 T7182 T7183 det the,absence
R5541 T7183 T7181 pobj absence,to
R5542 T7184 T7183 prep of,absence
R5543 T7185 T7186 compound HOIP,expression
R5544 T7186 T7184 pobj expression,of
R5545 T7187 T7157 punct .,were
R5546 T7188 T7189 det These,data
R5547 T7189 T7190 nsubj data,indicate
R5548 T7190 T7190 ROOT indicate,indicate
R5549 T7191 T7194 mark that,required
R5550 T7192 T7194 nsubjpass HOIP,required
R5551 T7193 T7194 auxpass is,required
R5552 T7194 T7190 ccomp required,indicate
R5553 T7195 T7194 prep for,required
R5554 T7196 T7195 pobj recruitment,for
R5555 T7197 T7196 prep of,recruitment
R5556 T7198 T7200 det the,complex
R5557 T7199 T7200 amod NF-κB-activating,complex
R5558 T7200 T7197 pobj complex,of
R5559 T7201 T7200 prep to,complex
R5560 T7202 T7203 det the,CD40
R5561 T7203 T7204 nsubj CD40,signaling
R5562 T7204 T7201 pcomp signaling,to
R5563 T7205 T7204 dobj complex,signaling
R5564 T7206 T7190 punct .,indicate
R5565 T7207 T7208 mark As,shown
R5566 T7208 T7208 ROOT shown,shown
R5567 T7209 T7208 advmod here,shown
R5568 T7210 T7208 cc and,shown
R5569 T7211 T7220 prep in,present
R5570 T7212 T7214 poss our,study
R5571 T7213 T7214 amod previous,study
R5572 T7214 T7211 pobj study,in
R5573 T7215 T7217 nmod [,]
R5574 T7216 T7217 nummod 6,]
R5575 T7217 T7220 nsubj ],present
R5576 T7218 T7220 punct ",",present
R5577 T7219 T7220 compound IKKγ,present
R5578 T7220 T7224 nsubj present,appears
R5579 T7221 T7220 prep in,present
R5580 T7222 T7221 pobj CD40,in
R5581 T7223 T7224 nsubj immunoprecipitates,appears
R5582 T7224 T7208 conj appears,shown
R5583 T7225 T7226 aux to,have
R5584 T7226 T7224 xcomp have,appears
R5585 T7227 T7230 det a,weight
R5586 T7228 T7230 amod higher,weight
R5587 T7229 T7230 amod molecular,weight
R5588 T7230 T7226 dobj weight,have
R5589 T7231 T7230 prep than,weight
R5590 T7232 T7231 pobj that,than
R5591 T7233 T7232 acl found,that
R5592 T7234 T7233 prep in,found
R5593 T7235 T7236 compound cell,lysates
R5594 T7236 T7234 pobj lysates,in
R5595 T7237 T7224 punct .,appears
R5596 T7238 T7239 aux To,confirm
R5597 T7239 T7251 advcl confirm,generated
R5598 T7240 T7246 mark that,was
R5599 T7241 T7245 det this,species
R5600 T7242 T7244 amod higher,weight
R5601 T7243 T7244 amod molecular,weight
R5602 T7244 T7245 compound weight,species
R5603 T7245 T7246 nsubj species,was
R5604 T7246 T7239 ccomp was,confirm
R5605 T7247 T7246 advmod indeed,was
R5606 T7248 T7246 attr IKKγ,was
R5607 T7249 T7251 punct ",",generated
R5608 T7250 T7251 nsubj we,generated
R5609 T7251 T7251 ROOT generated,generated
R5610 T7252 T7256 compound A20,lines
R5611 T7253 T7254 compound .2,J
R5612 T7254 T7256 compound J,lines
R5613 T7255 T7256 compound cell,lines
R5614 T7256 T7251 dobj lines,generated
R5615 T7257 T7256 acl containing,lines
R5616 T7258 T7262 det a,vector
R5617 T7259 T7260 advmod stably,integrated
R5618 T7260 T7262 amod integrated,vector
R5619 T7261 T7262 amod retroviral,vector
R5620 T7262 T7257 dobj vector,containing
R5621 T7263 T7264 nsubj that,encoded
R5622 T7264 T7262 relcl encoded,vector
R5623 T7265 T7267 det an,version
R5624 T7266 T7267 amod epitope-tagged,version
R5625 T7267 T7264 dobj version,encoded
R5626 T7268 T7267 prep of,version
R5627 T7269 T7268 pobj mouse,of
R5628 T7270 T7267 appos IKKγ,version
R5629 T7271 T7251 punct .,generated
R5630 T7272 T7273 compound Cell,lysates
R5631 T7273 T7282 nsubjpass lysates,analyzed
R5632 T7274 T7273 cc and,lysates
R5633 T7275 T7276 amod CD40,immunoprecipitates
R5634 T7276 T7273 conj immunoprecipitates,lysates
R5635 T7277 T7273 acl prepared,lysates
R5636 T7278 T7277 prep from,prepared
R5637 T7279 T7280 det these,cells
R5638 T7280 T7278 pobj cells,from
R5639 T7281 T7282 auxpass were,analyzed
R5640 T7282 T7282 ROOT analyzed,analyzed
R5641 T7283 T7282 agent by,analyzed
R5642 T7284 T7285 amod Western,blotting
R5643 T7285 T7283 pobj blotting,by
R5644 T7286 T7282 prep for,analyzed
R5645 T7287 T7289 det the,tag
R5646 T7288 T7289 compound epitope,tag
R5647 T7289 T7286 pobj tag,for
R5648 T7290 T7289 punct (,tag
R5649 T7291 T7292 compound Fig.,5C
R5650 T7292 T7289 appos 5C,tag
R5651 T7293 T7289 punct ),tag
R5652 T7294 T7282 punct .,analyzed
R5653 T7295 T7296 det This,analysis
R5654 T7296 T7297 nsubj analysis,produced
R5655 T7297 T7297 ROOT produced,produced
R5656 T7298 T7299 det a,pattern
R5657 T7299 T7297 dobj pattern,produced
R5658 T7300 T7299 prep of,pattern
R5659 T7301 T7300 pobj bands,of
R5660 T7302 T7303 nsubj that,was
R5661 T7303 T7299 relcl was,pattern
R5662 T7304 T7303 advmod essentially,was
R5663 T7305 T7306 det the,same
R5664 T7306 T7303 attr same,was
R5665 T7307 T7306 prep as,same
R5666 T7308 T7309 det that,obtained
R5667 T7309 T7307 pobj obtained,as
R5668 T7310 T7303 advcl using,was
R5669 T7311 T7313 det an,specific
R5670 T7312 T7313 compound antibody,specific
R5671 T7313 T7310 dobj specific,using
R5672 T7314 T7313 prep for,specific
R5673 T7315 T7316 amod native,IKKγ
R5674 T7316 T7314 pobj IKKγ,for
R5675 T7317 T7318 punct (,compare
R5676 T7318 T7310 xcomp compare,using
R5677 T7319 T7318 dobj panels,compare
R5678 T7320 T7318 conj B,compare
R5679 T7321 T7320 cc and,B
R5680 T7322 T7320 conj C,B
R5681 T7323 T7318 prep in,compare
R5682 T7324 T7323 pobj Fig.,in
R5683 T7325 T7324 nummod 5,Fig.
R5684 T7326 T7318 punct ),compare
R5685 T7327 T7297 punct .,produced
R5686 T7328 T7336 advmod Moreover,confirmed
R5687 T7329 T7336 punct ",",confirmed
R5688 T7330 T7336 nsubj analysis,confirmed
R5689 T7331 T7330 prep of,analysis
R5690 T7332 T7331 pobj cells,of
R5691 T7333 T7332 acl expressing,cells
R5692 T7334 T7335 compound epitope,tagged-IKKγ
R5693 T7335 T7333 dobj tagged-IKKγ,expressing
R5694 T7336 T7336 ROOT confirmed,confirmed
R5695 T7337 T7343 mark that,requires
R5696 T7338 T7343 nsubj recruitment,requires
R5697 T7339 T7338 prep of,recruitment
R5698 T7340 T7339 pobj IKKγ,of
R5699 T7341 T7338 prep to,recruitment
R5700 T7342 T7341 pobj CD40,to
R5701 T7343 T7336 ccomp requires,confirmed
R5702 T7344 T7343 dobj HOIP,requires
R5703 T7345 T7336 punct .,confirmed
R5704 T7346 T7347 aux To,determine
R5705 T7347 T7366 advcl determine,incubated
R5706 T7348 T7349 nsubj what,was
R5707 T7349 T7347 ccomp was,determine
R5708 T7350 T7349 acomp responsible,was
R5709 T7351 T7350 prep for,responsible
R5710 T7352 T7355 det the,weight
R5711 T7353 T7355 amod increased,weight
R5712 T7354 T7355 amod molecular,weight
R5713 T7355 T7351 pobj weight,for
R5714 T7356 T7355 prep of,weight
R5715 T7357 T7356 pobj IKKγ,of
R5716 T7358 T7355 prep in,weight
R5717 T7359 T7360 compound CD40,immunoprecipitates
R5718 T7360 T7358 pobj immunoprecipitates,in
R5719 T7361 T7366 punct ",",incubated
R5720 T7362 T7364 det the,samples
R5721 T7363 T7364 compound protein,samples
R5722 T7364 T7366 nsubjpass samples,incubated
R5723 T7365 T7366 auxpass were,incubated
R5724 T7366 T7366 ROOT incubated,incubated
R5725 T7367 T7366 prep with,incubated
R5726 T7368 T7369 compound lambda,phosphatase
R5727 T7369 T7367 pobj phosphatase,with
R5728 T7370 T7369 punct ",",phosphatase
R5729 T7371 T7372 nsubj which,removes
R5730 T7372 T7369 relcl removes,phosphatase
R5731 T7373 T7372 dobj phosphates,removes
R5732 T7374 T7373 acl attached,phosphates
R5733 T7375 T7376 aux to,tyrosine
R5734 T7376 T7374 xcomp tyrosine,attached
R5735 T7377 T7376 punct ",",tyrosine
R5736 T7378 T7376 conj threonine,tyrosine
R5737 T7379 T7378 punct ",",threonine
R5738 T7380 T7378 cc or,threonine
R5739 T7381 T7378 conj serine,threonine
R5740 T7382 T7378 conj residues,threonine
R5741 T7383 T7382 prep in,residues
R5742 T7384 T7383 pobj proteins,in
R5743 T7385 T7366 punct .,incubated
R5744 T7386 T7387 det This,treatment
R5745 T7387 T7388 nsubj treatment,reduced
R5746 T7388 T7388 ROOT reduced,reduced
R5747 T7389 T7388 dobj much,reduced
R5748 T7390 T7389 prep of,much
R5749 T7391 T7392 compound CD40-associated,IKKγ
R5750 T7392 T7390 pobj IKKγ,of
R5751 T7393 T7388 prep to,reduced
R5752 T7394 T7397 det an,weight
R5753 T7395 T7397 amod apparent,weight
R5754 T7396 T7397 amod molecular,weight
R5755 T7397 T7393 pobj weight,to
R5756 T7398 T7397 amod similar,weight
R5757 T7399 T7398 prep to,similar
R5758 T7400 T7399 pobj that,to
R5759 T7401 T7400 prep of,that
R5760 T7402 T7403 det the,protein
R5761 T7403 T7401 pobj protein,of
R5762 T7404 T7398 prep in,similar
R5763 T7405 T7406 compound cell,lysates
R5764 T7406 T7404 pobj lysates,in
R5765 T7407 T7409 punct (,5C
R5766 T7408 T7409 compound Fig.,5C
R5767 T7409 T7398 appos 5C,similar
R5768 T7410 T7409 punct ),5C
R5769 T7411 T7388 punct .,reduced
R5770 T7412 T7423 advmod However,remained
R5771 T7413 T7423 punct ",",remained
R5772 T7414 T7415 advmod at,least
R5773 T7415 T7416 advmod least,one
R5774 T7416 T7417 nummod one,band
R5775 T7417 T7423 nsubj band,remained
R5776 T7418 T7417 prep of,band
R5777 T7419 T7420 advmod significantly,higher
R5778 T7420 T7422 amod higher,weight
R5779 T7421 T7422 amod molecular,weight
R5780 T7422 T7418 pobj weight,of
R5781 T7423 T7423 ROOT remained,remained
R5782 T7424 T7423 prep after,remained
R5783 T7425 T7426 compound phosphatase,treatment
R5784 T7426 T7424 pobj treatment,after
R5785 T7427 T7423 punct ",",remained
R5786 T7428 T7423 advcl suggesting,remained
R5787 T7429 T7432 mark that,is
R5788 T7430 T7431 compound CD40-associated,IKKγ
R5789 T7431 T7432 nsubj IKKγ,is
R5790 T7432 T7428 ccomp is,suggesting
R5791 T7433 T7432 acomp subject,is
R5792 T7434 T7433 prep to,subject
R5793 T7435 T7436 advmod at,least
R5794 T7436 T7437 advmod least,one
R5795 T7437 T7439 nummod one,modification
R5796 T7438 T7439 amod other,modification
R5797 T7439 T7434 pobj modification,to
R5798 T7440 T7439 prep in,modification
R5799 T7441 T7440 pobj addition,in
R5800 T7442 T7441 prep to,addition
R5801 T7443 T7442 pobj phosphorylation,to
R5802 T7444 T7423 punct .,remained
R5803 T7445 T7449 advmod Overall,demonstrate
R5804 T7446 T7449 punct ",",demonstrate
R5805 T7447 T7448 det these,data
R5806 T7448 T7449 nsubj data,demonstrate
R5807 T7449 T7449 ROOT demonstrate,demonstrate
R5808 T7450 T7453 mark that,required
R5809 T7451 T7453 nsubjpass HOIP,required
R5810 T7452 T7453 auxpass is,required
R5811 T7453 T7449 ccomp required,demonstrate
R5812 T7454 T7453 prep for,required
R5813 T7455 T7456 det the,association
R5814 T7456 T7454 pobj association,for
R5815 T7457 T7456 prep of,association
R5816 T7458 T7457 pobj IKKγ,of
R5817 T7459 T7456 prep with,association
R5818 T7460 T7459 pobj CD40,with
R5819 T7461 T7453 punct ",",required
R5820 T7462 T7453 cc and,required
R5821 T7463 T7449 conj suggest,demonstrate
R5822 T7464 T7468 mark that,coupled
R5823 T7465 T7466 det this,event
R5824 T7466 T7468 nsubjpass event,coupled
R5825 T7467 T7468 auxpass is,coupled
R5826 T7468 T7463 ccomp coupled,suggest
R5827 T7469 T7468 prep to,coupled
R5828 T7470 T7471 amod post-translational,modifications
R5829 T7471 T7469 pobj modifications,to
R5830 T7472 T7471 prep of,modifications
R5831 T7473 T7472 pobj IKKγ,of
R5832 T7474 T7449 punct .,demonstrate
R11703 T14807 T14808 compound HOIP,gene
R11704 T14808 T14809 compound gene,targeting
R11705 T14809 T14809 ROOT targeting,targeting
R11706 T14810 T14809 punct .,targeting
R11707 T14811 T14833 punct (,shown
R11708 T14812 T14833 advmod A,shown
R11709 T14813 T14814 punct ),Regions
R11710 T14814 T14833 nsubjpass Regions,shown
R11711 T14815 T14814 prep of,Regions
R11712 T14816 T14818 amod Rnf31,sequence
R11713 T14817 T14818 compound gene,sequence
R11714 T14818 T14815 pobj sequence,of
R11715 T14819 T14818 punct (,sequence
R11716 T14820 T14818 appos bottom,sequence
R11717 T14821 T14818 punct ),sequence
R11718 T14822 T14814 acl used,Regions
R11719 T14823 T14822 prep in,used
R11720 T14824 T14826 det the,gene
R11721 T14825 T14826 compound HOIP,gene
R11722 T14826 T14823 pobj gene,in
R11723 T14827 T14814 acl targeting,Regions
R11724 T14828 T14827 dobj vector,targeting
R11725 T14829 T14828 punct (,vector
R11726 T14830 T14828 amod top,vector
R11727 T14831 T14814 punct ),Regions
R11728 T14832 T14833 auxpass are,shown
R11729 T14833 T14833 ROOT shown,shown
R11730 T14834 T14833 punct .,shown
R11731 T14835 T14841 nsubj Arrows,indicate
R11732 T14836 T14837 punct (,F
R11733 T14837 T14835 appos F,Arrows
R11734 T14838 T14837 cc and,F
R11735 T14839 T14837 conj R,F
R11736 T14840 T14835 punct ),Arrows
R11737 T14841 T14841 ROOT indicate,indicate
R11738 T14842 T14843 amod approximate,positions
R11739 T14843 T14841 dobj positions,indicate
R11740 T14844 T14843 prep of,positions
R11741 T14845 T14844 pobj sequences,of
R11742 T14846 T14841 advmod homologous,indicate
R11743 T14847 T14846 prep to,homologous
R11744 T14848 T14847 pobj oligonucleotides,to
R11745 T14849 T14848 acl used,oligonucleotides
R11746 T14850 T14849 prep for,used
R11747 T14851 T14852 amod PCR-mediated,detection
R11748 T14852 T14850 pobj detection,for
R11749 T14853 T14852 prep of,detection
R11750 T14854 T14855 amod homologous,recombination
R11751 T14855 T14853 pobj recombination,of
R11752 T14856 T14841 punct .,indicate
R11753 T14857 T14858 amod Homologous,recombination
R11754 T14858 T14864 nsubj recombination,resulted
R11755 T14859 T14858 prep of,recombination
R11756 T14860 T14861 det the,vector
R11757 T14861 T14859 pobj vector,of
R11758 T14862 T14858 prep with,recombination
R11759 T14863 T14862 pobj Rnf31,with
R11760 T14864 T14864 ROOT resulted,resulted
R11761 T14865 T14864 prep in,resulted
R11762 T14866 T14869 det a,deletion
R11763 T14867 T14869 amod small,deletion
R11764 T14868 T14869 amod chromosomal,deletion
R11765 T14869 T14865 pobj deletion,in
R11766 T14870 T14869 cc and,deletion
R11767 T14871 T14873 det the,insertion
R11768 T14872 T14873 amod in-frame,insertion
R11769 T14873 T14869 conj insertion,deletion
R11770 T14874 T14873 prep of,insertion
R11771 T14875 T14876 compound neomycin,phosphotransferase
R11772 T14876 T14874 pobj phosphotransferase,of
R11773 T14877 T14878 punct (,NeoR
R11774 T14878 T14876 appos NeoR,phosphotransferase
R11775 T14879 T14864 punct ),resulted
R11776 T14880 T14864 prep into,resulted
R11777 T14881 T14885 det the,sequence
R11778 T14882 T14885 amod amino-terminal,sequence
R11779 T14883 T14885 compound HOIP,sequence
R11780 T14884 T14885 compound coding,sequence
R11781 T14885 T14880 pobj sequence,into
R11782 T14886 T14864 punct .,resulted
R11783 T14887 T14889 det A,toxin
R11784 T14888 T14889 compound diphtheria,toxin
R11785 T14889 T14897 nsubj toxin,facilitates
R11786 T14890 T14889 punct (,toxin
R11787 T14891 T14889 appos DT,toxin
R11788 T14892 T14889 punct ),toxin
R11789 T14893 T14889 appos cassette,toxin
R11790 T14894 T14893 prep in,cassette
R11791 T14895 T14896 det the,vector
R11792 T14896 T14894 pobj vector,in
R11793 T14897 T14897 ROOT facilitates,facilitates
R11794 T14898 T14900 det the,selection
R11795 T14899 T14900 amod negative,selection
R11796 T14900 T14897 dobj selection,facilitates
R11797 T14901 T14900 prep of,selection
R11798 T14902 T14901 pobj cells,of
R11799 T14903 T14911 prep in,takes
R11800 T14904 T14903 pobj which,in
R11801 T14905 T14907 amod random,insertion
R11802 T14906 T14907 amod chromosomal,insertion
R11803 T14907 T14911 nsubj insertion,takes
R11804 T14908 T14907 prep of,insertion
R11805 T14909 T14910 det the,vector
R11806 T14910 T14908 pobj vector,of
R11807 T14911 T14900 relcl takes,selection
R11808 T14912 T14911 dobj place,takes
R11809 T14913 T14897 punct .,facilitates
R11810 T14914 T14915 compound LoxP,sequences
R11811 T14915 T14916 nsubj sequences,allow
R11812 T14916 T14916 ROOT allow,allow
R11813 T14917 T14919 det the,deletion
R11814 T14918 T14919 amod Cre-mediated,deletion
R11815 T14919 T14916 dobj deletion,allow
R11816 T14920 T14919 prep of,deletion
R11817 T14921 T14924 det the,sequence
R11818 T14922 T14924 compound NeoR,sequence
R11819 T14923 T14924 compound coding,sequence
R11820 T14924 T14920 pobj sequence,of
R11821 T14925 T14916 punct .,allow
R11822 T14926 T14928 det The,sequence
R11823 T14927 T14928 compound SV40pA,sequence
R11824 T14928 T14929 nsubj sequence,helps
R11825 T14929 T14929 ROOT helps,helps
R11826 T14930 T14931 aux to,ensure
R11827 T14931 T14929 xcomp ensure,helps
R11828 T14932 T14931 dobj disruption,ensure
R11829 T14933 T14932 prep of,disruption
R11830 T14934 T14935 compound gene,expression
R11831 T14935 T14933 pobj expression,of
R11832 T14936 T14931 prep after,ensure
R11833 T14937 T14936 pobj deletion,after
R11834 T14938 T14937 prep of,deletion
R11835 T14939 T14941 det the,sequence
R11836 T14940 T14941 compound NeoR,sequence
R11837 T14941 T14938 pobj sequence,of
R11838 T14942 T14929 punct .,helps
R11839 T14943 T14946 punct (,Anti-HOIP
R11840 T14944 T14946 nmod B,Anti-HOIP
R11841 T14945 T14946 punct ),Anti-HOIP
R11842 T14946 T14950 nmod Anti-HOIP,blots
R11843 T14947 T14946 cc and,Anti-HOIP
R11844 T14948 T14949 compound anti-FLAG,Western
R11845 T14949 T14946 conj Western,Anti-HOIP
R11846 T14950 T14950 ROOT blots,blots
R11847 T14951 T14950 prep of,blots
R11848 T14952 T14953 compound cell,lysates
R11849 T14953 T14951 pobj lysates,of
R11850 T14954 T14950 prep from,blots
R11851 T14955 T14958 compound A20,cells
R11852 T14956 T14957 compound .2,J
R11853 T14957 T14958 compound J,cells
R11854 T14958 T14954 pobj cells,from
R11855 T14959 T14958 cc and,cells
R11856 T14960 T14958 conj HOIP-deficient,cells
R11857 T14961 T14967 punct (,clones
R11858 T14962 T14967 dep HOIP,clones
R11859 T14963 T14967 punct -,clones
R11860 T14964 T14967 amod /,clones
R11861 T14965 T14964 punct -,/
R11862 T14966 T14964 punct ),/
R11863 T14967 T14958 conj clones,cells
R11864 T14968 T14950 punct .,blots
R11865 T14969 T14971 det A,decrease
R11866 T14970 T14971 amod partial,decrease
R11867 T14971 T14976 nsubj decrease,is
R11868 T14972 T14971 prep in,decrease
R11869 T14973 T14975 compound HOIP,expression
R11870 T14974 T14975 compound protein,expression
R11871 T14975 T14972 pobj expression,in
R11872 T14976 T14976 ROOT is,is
R11873 T14977 T14976 acomp evident,is
R11874 T14978 T14976 prep in,is
R11875 T14979 T14978 pobj cells,in
R11876 T14980 T14979 acl following,cells
R11877 T14981 T14980 dobj disruption,following
R11878 T14982 T14981 prep of,disruption
R11879 T14983 T14984 nummod one,copy
R11880 T14984 T14982 pobj copy,of
R11881 T14985 T14984 prep of,copy
R11882 T14986 T14985 pobj Rnf31,of
R11883 T14987 T14986 punct (,Rnf31
R11884 T14988 T14990 neg HOIP,/
R11885 T14989 T14990 compound −,/
R11886 T14990 T14986 parataxis /,Rnf31
R11887 T14991 T14986 appos +,Rnf31
R11888 T14992 T14986 punct ),Rnf31
R11889 T14993 T14976 punct .,is
R11890 T14994 T14995 compound HOIP,expression
R11891 T14995 T15016 nsubjpass expression,shown
R11892 T14996 T14995 prep in,expression
R11893 T14997 T14996 pobj clones,in
R11894 T14998 T14997 acl reconstituted,clones
R11895 T14999 T14998 prep with,reconstituted
R11896 T15000 T15003 det an,vector
R11897 T15001 T15003 amod empty,vector
R11898 T15002 T15003 compound retroviral,vector
R11899 T15003 T14999 pobj vector,with
R11900 T15004 T15003 punct (,vector
R11901 T15005 T15003 appos pMIP,vector
R11902 T15006 T15003 punct ),vector
R11903 T15007 T15003 cc or,vector
R11904 T15008 T15010 det a,vector
R11905 T15009 T15010 amod retroviral,vector
R11906 T15010 T15003 conj vector,vector
R11907 T15011 T15010 acl encoding,vector
R11908 T15012 T15013 compound FLAG-tagged,HOIP
R11909 T15013 T15011 dobj HOIP,encoding
R11910 T15014 T15016 auxpass is,shown
R11911 T15015 T15016 advmod also,shown
R11912 T15016 T15016 ROOT shown,shown
R11913 T15017 T15016 punct .,shown
R11914 T15018 T15020 amod Approximate,weight
R11915 T15019 T15020 amod molecular,weight
R11916 T15020 T15023 nsubj weight,is
R11917 T15021 T15020 prep of,weight
R11918 T15022 T15021 pobj HOIP,of
R11919 T15023 T15023 ROOT is,is
R11920 T15024 T15025 nummod 120,kD
R11921 T15025 T15023 attr kD,is
R11922 T15026 T15023 punct .,is
R12180 T15392 T15394 amod CD40-mediated,upregulation
R12181 T15393 T15394 compound CD80,upregulation
R12182 T15394 T15395 nsubj upregulation,is
R12183 T15395 T15395 ROOT is,is
R12184 T15396 T15395 acomp defective,is
R12185 T15397 T15396 prep in,defective
R12186 T15398 T15399 amod HOIP-deficient,cells
R12187 T15399 T15397 pobj cells,in
R12188 T15400 T15395 punct .,is
R12189 T15401 T15403 nsubjpass Cells,incubated
R12190 T15402 T15403 auxpass were,incubated
R12191 T15403 T15403 ROOT incubated,incubated
R12192 T15404 T15403 prep for,incubated
R12193 T15405 T15406 nummod 72,hrs
R12194 T15406 T15404 pobj hrs,for
R12195 T15407 T15403 prep with,incubated
R12196 T15408 T15411 det an,antibody
R12197 T15409 T15410 amod isotype,control
R12198 T15410 T15411 compound control,antibody
R12199 T15411 T15407 pobj antibody,with
R12200 T15412 T15413 punct (,left
R12201 T15413 T15403 advcl left,incubated
R12202 T15414 T15413 punct ),left
R12203 T15415 T15413 cc or,left
R12204 T15416 T15417 amod anti-CD40,antibody
R12205 T15417 T15403 conj antibody,incubated
R12206 T15418 T15419 punct (,right
R12207 T15419 T15417 parataxis right,antibody
R12208 T15420 T15419 punct ),right
R12209 T15421 T15403 cc and,incubated
R12210 T15422 T15423 advmod then,stained
R12211 T15423 T15403 conj stained,incubated
R12212 T15424 T15423 prep for,stained
R12213 T15425 T15424 pobj expression,for
R12214 T15426 T15425 prep of,expression
R12215 T15427 T15426 pobj CD80,of
R12216 T15428 T15429 punct (,filled
R12217 T15429 T15430 amod filled,profiles
R12218 T15430 T15431 nsubj profiles,indicate
R12219 T15431 T15423 ccomp indicate,stained
R12220 T15432 T15431 ccomp staining,indicate
R12221 T15433 T15432 prep with,staining
R12222 T15434 T15435 amod anti-CD80,antibody
R12223 T15435 T15433 pobj antibody,with
R12224 T15436 T15431 punct ;,indicate
R12225 T15437 T15438 amod open,profiles
R12226 T15438 T15440 nsubj profiles,staining
R12227 T15439 T15440 aux are,staining
R12228 T15440 T15403 conj staining,incubated
R12229 T15441 T15440 prep with,staining
R12230 T15442 T15445 det an,antibody
R12231 T15443 T15444 amod isotype,control
R12232 T15444 T15445 compound control,antibody
R12233 T15445 T15441 pobj antibody,with
R12234 T15446 T15440 punct ),staining
R12235 T15447 T15440 punct .,staining
R12236 T15448 T15464 nsubj Transduction,reversed
R12237 T15449 T15448 prep of,Transduction
R12238 T15450 T15451 det both,HOIP-deficient
R12239 T15451 T15449 pobj HOIP-deficient,of
R12240 T15452 T15451 punct (,HOIP-deficient
R12241 T15453 T15455 amod HOIP,/
R12242 T15454 T15455 punct -,/
R12243 T15455 T15455 ROOT /,/
R12244 T15456 T15455 punct -,/
R12245 T15457 T15455 punct ),/
R12246 T15458 T15464 nsubj clones,reversed
R12247 T15459 T15458 prep with,clones
R12248 T15460 T15461 det a,retrovirus
R12249 T15461 T15459 pobj retrovirus,with
R12250 T15462 T15461 acl encoding,retrovirus
R12251 T15463 T15464 nsubj FLAG-HOIP,reversed
R12252 T15464 T15464 ROOT reversed,reversed
R12253 T15465 T15467 amod defective,upregulation
R12254 T15466 T15467 compound CD80,upregulation
R12255 T15467 T15464 dobj upregulation,reversed
R12256 T15468 T15464 punct ",",reversed
R12257 T15469 T15470 mark while,transduction
R12258 T15470 T15464 advcl transduction,reversed
R12259 T15471 T15470 prep with,transduction
R12260 T15472 T15474 det an,vector
R12261 T15473 T15474 amod empty,vector
R12262 T15474 T15471 pobj vector,with
R12263 T15475 T15474 punct (,vector
R12264 T15476 T15474 appos pMIP,vector
R12265 T15477 T15474 punct ),vector
R12266 T15478 T15480 aux did,.
R12267 T15479 T15478 neg not,did
R12268 T15480 T15464 punct .,reversed
R12269 T15481 T15482 amod Similar,results
R12270 T15482 T15484 nsubjpass results,obtained
R12271 T15483 T15484 auxpass were,obtained
R12272 T15484 T15484 ROOT obtained,obtained
R12273 T15485 T15484 prep in,obtained
R12274 T15486 T15488 det a,experiment
R12275 T15487 T15488 amod second,experiment
R12276 T15488 T15485 pobj experiment,in
R12277 T15489 T15484 punct .,obtained
R12429 T15742 T15743 compound GLε,transcription
R12430 T15743 T15744 nsubj transcription,is
R12431 T15744 T15744 ROOT is,is
R12432 T15745 T15744 acomp defective,is
R12433 T15746 T15745 prep in,defective
R12434 T15747 T15749 amod CD40-stimulated,cells
R12435 T15748 T15749 amod HOIP-deficient,cells
R12436 T15749 T15746 pobj cells,in
R12437 T15750 T15744 punct .,is
R12438 T15751 T15753 nummod A20,J
R12439 T15752 T15753 nummod .2,J
R12440 T15753 T15756 nmod J,cells
R12441 T15754 T15753 cc and,J
R12442 T15755 T15753 conj HOIP-deficient,J
R12443 T15756 T15756 ROOT cells,cells
R12444 T15757 T15756 acl transduced,cells
R12445 T15758 T15757 prep with,transduced
R12446 T15759 T15762 det an,vector
R12447 T15760 T15762 amod empty,vector
R12448 T15761 T15762 compound retroviral,vector
R12449 T15762 T15758 pobj vector,with
R12450 T15763 T15762 punct (,vector
R12451 T15764 T15768 nummod A20,pMIP
R12452 T15765 T15768 nummod .2,pMIP
R12453 T15766 T15768 nmod J,pMIP
R12454 T15767 T15768 nummod +,pMIP
R12455 T15768 T15762 appos pMIP,vector
R12456 T15769 T15768 cc and,pMIP
R12457 T15770 T15768 conj HOIP,pMIP
R12458 T15771 T15762 punct -,vector
R12459 T15772 T15762 punct /,vector
R12460 T15773 T15762 punct -,vector
R12461 T15774 T15775 compound +,pMIP
R12462 T15775 T15762 appos pMIP,vector
R12463 T15776 T15775 punct ",",pMIP
R12464 T15777 T15775 advmod respectively,pMIP
R12465 T15778 T15775 punct ),pMIP
R12466 T15779 T15775 punct ",",pMIP
R12467 T15780 T15775 cc or,pMIP
R12468 T15781 T15782 amod HOIP-deficient,cells
R12469 T15782 T15775 conj cells,pMIP
R12470 T15783 T15762 acl transduced,vector
R12471 T15784 T15783 prep with,transduced
R12472 T15785 T15787 det a,retrovirus
R12473 T15786 T15787 amod HOIP-encoding,retrovirus
R12474 T15787 T15784 pobj retrovirus,with
R12475 T15788 T15797 punct (,cultured
R12476 T15789 T15793 punct HOIP,+
R12477 T15790 T15793 punct -,+
R12478 T15791 T15793 punct /,+
R12479 T15792 T15793 punct -,+
R12480 T15793 T15797 nsubjpass +,cultured
R12481 T15794 T15793 npadvmod HOIP,+
R12482 T15795 T15797 punct ),cultured
R12483 T15796 T15797 auxpass were,cultured
R12484 T15797 T15787 parataxis cultured,retrovirus
R12485 T15798 T15797 npadvmod overnight,cultured
R12486 T15799 T15797 prep with,cultured
R12487 T15800 T15801 amod agonistic,anti-CD40
R12488 T15801 T15799 pobj anti-CD40,with
R12489 T15802 T15801 cc or,anti-CD40
R12490 T15803 T15806 det an,antibody
R12491 T15804 T15806 nmod isotype,antibody
R12492 T15805 T15806 compound control,antibody
R12493 T15806 T15801 conj antibody,anti-CD40
R12494 T15807 T15806 punct (,antibody
R12495 T15808 T15806 parataxis iso,antibody
R12496 T15809 T15808 punct ),iso
R12497 T15810 T15806 punct ",",antibody
R12498 T15811 T15806 conj with,antibody
R12499 T15812 T15811 cc or,with
R12500 T15813 T15811 conj without,with
R12501 T15814 T15813 pobj IL-4,without
R12502 T15815 T15756 punct .,cells
R12503 T15816 T15818 nsubjpass RNA,isolated
R12504 T15817 T15818 auxpass was,isolated
R12505 T15818 T15818 ROOT isolated,isolated
R12506 T15819 T15818 prep from,isolated
R12507 T15820 T15821 det the,cells
R12508 T15821 T15819 pobj cells,from
R12509 T15822 T15818 punct ",",isolated
R12510 T15823 T15818 conj reverse-transcribed,isolated
R12511 T15824 T15823 punct ",",reverse-transcribed
R12512 T15825 T15823 cc and,reverse-transcribed
R12513 T15826 T15827 advmod then,subjected
R12514 T15827 T15823 conj subjected,reverse-transcribed
R12515 T15828 T15827 prep to,subjected
R12516 T15829 T15830 amod quantitative,PCR
R12517 T15830 T15828 pobj PCR,to
R12518 T15831 T15832 aux to,determine
R12519 T15832 T15827 advcl determine,subjected
R12520 T15833 T15832 dobj levels,determine
R12521 T15834 T15833 prep of,levels
R12522 T15835 T15836 compound GLε,transcripts
R12523 T15836 T15834 pobj transcripts,of
R12524 T15837 T15818 punct .,isolated
R12525 T15838 T15839 nsubj Results,were
R12526 T15839 T15839 ROOT were,were
R12527 T15840 T15839 acomp normalized,were
R12528 T15841 T15840 prep to,normalized
R12529 T15842 T15843 det the,levels
R12530 T15843 T15841 pobj levels,to
R12531 T15844 T15843 prep of,levels
R12532 T15845 T15846 amod Hprt1,transcripts
R12533 T15846 T15844 pobj transcripts,of
R12534 T15847 T15843 prep in,levels
R12535 T15848 T15849 det each,sample
R12536 T15849 T15847 pobj sample,in
R12537 T15850 T15839 punct .,were
R12538 T15851 T15852 nsubj Symbols,indicate
R12539 T15852 T15852 ROOT indicate,indicate
R12540 T15853 T15854 det the,values
R12541 T15854 T15852 dobj values,indicate
R12542 T15855 T15852 prep from,indicate
R12543 T15856 T15857 compound duplicate,cultures
R12544 T15857 T15855 pobj cultures,from
R12545 T15858 T15857 punct (,cultures
R12546 T15859 T15860 det a,line
R12547 T15860 T15861 nsubj line,indicates
R12548 T15861 T15852 ccomp indicates,indicate
R12549 T15862 T15863 det the,mean
R12550 T15863 T15861 dobj mean,indicates
R12551 T15864 T15863 prep of,mean
R12552 T15865 T15867 det the,values
R12553 T15866 T15867 nummod two,values
R12554 T15867 T15864 pobj values,of
R12555 T15868 T15861 punct ),indicates
R12556 T15869 T15852 punct .,indicate
R12557 T15870 T15871 amod Similar,results
R12558 T15871 T15873 nsubjpass results,obtained
R12559 T15872 T15873 auxpass were,obtained
R12560 T15873 T15873 ROOT obtained,obtained
R12561 T15874 T15873 prep in,obtained
R12562 T15875 T15877 det a,experiment
R12563 T15876 T15877 amod second,experiment
R12564 T15877 T15874 pobj experiment,in
R12565 T15878 T15874 cc and,in
R12566 T15879 T15874 conj in,in
R12567 T15880 T15882 det an,experiment
R12568 T15881 T15882 amod additional,experiment
R12569 T15882 T15879 pobj experiment,in
R12570 T15883 T15882 prep with,experiment
R12571 T15884 T15887 det a,clone
R12572 T15885 T15887 amod second,clone
R12573 T15886 T15887 amod HOIP-deficient,clone
R12574 T15887 T15883 pobj clone,with
R12575 T15888 T15873 punct .,obtained
R12757 T16192 T16193 amod CD40-induced,activation
R12758 T16193 T16198 nsubj activation,is
R12759 T16194 T16193 prep of,activation
R12760 T16195 T16194 pobj NF-κB,of
R12761 T16196 T16195 cc and,NF-κB
R12762 T16197 T16195 conj JNK,NF-κB
R12763 T16198 T16198 ROOT is,is
R12764 T16199 T16198 acomp defective,is
R12765 T16200 T16199 prep in,defective
R12766 T16201 T16202 amod HOIP-deficient,cells
R12767 T16202 T16200 pobj cells,in
R12768 T16203 T16198 punct .,is
R12769 T16204 T16205 amod CD40-mediated,signaling
R12770 T16205 T16206 nsubj signaling,was
R12771 T16206 T16206 ROOT was,was
R12772 T16207 T16206 acomp defective,was
R12773 T16208 T16207 prep in,defective
R12774 T16209 T16210 amod HOIP-deficient,cells
R12775 T16210 T16208 pobj cells,in
R12776 T16211 T16216 punct (,+
R12777 T16212 T16216 punct HOIP,+
R12778 T16213 T16216 punct -,+
R12779 T16214 T16216 punct /,+
R12780 T16215 T16216 punct -,+
R12781 T16216 T16206 parataxis +,was
R12782 T16217 T16216 npadvmod pMIP,+
R12783 T16218 T16216 punct ),+
R12784 T16219 T16216 punct ",",+
R12785 T16220 T16216 cc but,+
R12786 T16221 T16216 conj intact,+
R12787 T16222 T16221 prep in,intact
R12788 T16223 T16224 amod parental,cells
R12789 T16224 T16222 pobj cells,in
R12790 T16225 T16216 conj transduced,+
R12791 T16226 T16225 prep with,transduced
R12792 T16227 T16230 det an,vector
R12793 T16228 T16230 amod empty,vector
R12794 T16229 T16230 compound retroviral,vector
R12795 T16230 T16226 pobj vector,with
R12796 T16231 T16230 punct (,vector
R12797 T16232 T16236 nummod A20,pMIP
R12798 T16233 T16236 nummod .2,pMIP
R12799 T16234 T16236 nmod J,pMIP
R12800 T16235 T16236 nummod +,pMIP
R12801 T16236 T16230 appos pMIP,vector
R12802 T16237 T16236 punct ),pMIP
R12803 T16238 T16226 cc and,with
R12804 T16239 T16225 prep in,transduced
R12805 T16240 T16241 amod HOIP-deficient,cells
R12806 T16241 T16239 pobj cells,in
R12807 T16242 T16225 conj transduced,transduced
R12808 T16243 T16242 prep with,transduced
R12809 T16244 T16246 det a,vector
R12810 T16245 T16246 amod retroviral,vector
R12811 T16246 T16243 pobj vector,with
R12812 T16247 T16248 compound encoding,HOIP
R12813 T16248 T16246 appos HOIP,vector
R12814 T16249 T16255 punct (,HOIP
R12815 T16250 T16255 compound HOIP,HOIP
R12816 T16251 T16254 punct -,+
R12817 T16252 T16254 punct /,+
R12818 T16253 T16254 punct -,+
R12819 T16254 T16255 compound +,HOIP
R12820 T16255 T16248 appos HOIP,HOIP
R12821 T16256 T16255 punct ),HOIP
R12822 T16257 T16206 punct .,was
R12823 T16258 T16260 nsubjpass Cells,activated
R12824 T16259 T16260 auxpass were,activated
R12825 T16260 T16260 ROOT activated,activated
R12826 T16261 T16260 prep with,activated
R12827 T16262 T16264 amod CD154-expressing,cells
R12828 T16263 T16264 compound insect,cells
R12829 T16264 T16261 pobj cells,with
R12830 T16265 T16260 prep for,activated
R12831 T16266 T16267 det the,times
R12832 T16267 T16265 pobj times,for
R12833 T16268 T16260 conj indicated,activated
R12834 T16269 T16260 punct .,activated
R12835 T16270 T16277 prep As,stimulated
R12836 T16271 T16273 det a,control
R12837 T16272 T16273 amod negative,control
R12838 T16273 T16270 pobj control,As
R12839 T16274 T16277 punct ",",stimulated
R12840 T16275 T16277 nsubjpass cells,stimulated
R12841 T16276 T16277 auxpass were,stimulated
R12842 T16277 T16277 ROOT stimulated,stimulated
R12843 T16278 T16277 prep with,stimulated
R12844 T16279 T16280 amod insect,cells
R12845 T16280 T16278 pobj cells,with
R12846 T16281 T16280 acl lacking,cells
R12847 T16282 T16281 dobj CD154,lacking
R12848 T16283 T16282 punct (,CD154
R12849 T16284 T16287 nummod 5,point
R12850 T16285 T16286 amod minute,time
R12851 T16286 T16287 compound time,point
R12852 T16287 T16282 appos point,CD154
R12853 T16288 T16289 advmod only,)
R12854 T16289 T16282 punct ),CD154
R12855 T16290 T16277 punct .,stimulated
R12856 T16291 T16292 amod Western,blots
R12857 T16292 T16297 nsubjpass blots,probed
R12858 T16293 T16292 prep of,blots
R12859 T16294 T16295 amod whole-cell,lysates
R12860 T16295 T16293 pobj lysates,of
R12861 T16296 T16297 auxpass were,probed
R12862 T16297 T16297 ROOT probed,probed
R12863 T16298 T16297 prep with,probed
R12864 T16299 T16301 det the,antibodies
R12865 T16300 T16301 amod indicated,antibodies
R12866 T16301 T16298 pobj antibodies,with
R12867 T16302 T16297 punct .,probed
R12868 T16303 T16312 nmod Phospho-IκBα,blots
R12869 T16304 T16305 punct (,pIκBα
R12870 T16305 T16303 appos pIκBα,Phospho-IκBα
R12871 T16306 T16305 punct ),pIκBα
R12872 T16307 T16303 cc and,Phospho-IκBα
R12873 T16308 T16303 conj phospho-JNK,Phospho-IκBα
R12874 T16309 T16308 punct (,phospho-JNK
R12875 T16310 T16308 appos pJNK,phospho-JNK
R12876 T16311 T16308 punct ),phospho-JNK
R12877 T16312 T16314 nsubjpass blots,stripped
R12878 T16313 T16314 auxpass were,stripped
R12879 T16314 T16314 ROOT stripped,stripped
R12880 T16315 T16314 cc and,stripped
R12881 T16316 T16314 conj reprobed,stripped
R12882 T16317 T16316 prep for,reprobed
R12883 T16318 T16319 amod total,IκBα
R12884 T16319 T16317 pobj IκBα,for
R12885 T16320 T16316 cc and,reprobed
R12886 T16321 T16322 compound total,JNK
R12887 T16322 T16316 conj JNK,reprobed
R12888 T16323 T16322 punct ",",JNK
R12889 T16324 T16322 conj respectively,JNK
R12890 T16325 T16328 punct (,recognize
R12891 T16326 T16327 amod anti-JNK,antibodies
R12892 T16327 T16328 nsubj antibodies,recognize
R12893 T16328 T16322 parataxis recognize,JNK
R12894 T16329 T16328 dobj p46,recognize
R12895 T16330 T16329 cc and,p46
R12896 T16331 T16329 conj p54,p46
R12897 T16332 T16322 conj isoforms,JNK
R12898 T16333 T16322 punct ),JNK
R12899 T16334 T16314 punct .,stripped
R12900 T16335 T16336 compound IκBα,blots
R12901 T16336 T16339 nsubjpass blots,reprobed
R12902 T16337 T16339 auxpass were,reprobed
R12903 T16338 T16337 advmod also,were
R12904 T16339 T16339 ROOT reprobed,reprobed
R12905 T16340 T16339 prep for,reprobed
R12906 T16341 T16343 nsubj actin,demonstrate
R12907 T16342 T16343 aux to,demonstrate
R12908 T16343 T16339 advcl demonstrate,reprobed
R12909 T16344 T16345 amod equal,lane
R12910 T16345 T16346 compound lane,loading
R12911 T16346 T16343 dobj loading,demonstrate
R12912 T16347 T16339 punct .,reprobed
R12913 T16348 T16349 amod Molecular,weights
R12914 T16349 T16351 nsubjpass weights,indicated
R12915 T16350 T16351 auxpass are,indicated
R12916 T16351 T16351 ROOT indicated,indicated
R12917 T16352 T16351 prep at,indicated
R12918 T16353 T16352 pobj right,at
R12919 T16354 T16351 punct .,indicated
R12920 T16355 T16356 amod Similar,results
R12921 T16356 T16358 nsubjpass results,obtained
R12922 T16357 T16358 auxpass were,obtained
R12923 T16358 T16358 ROOT obtained,obtained
R12924 T16359 T16358 prep in,obtained
R12925 T16360 T16362 det a,experiment
R12926 T16361 T16362 amod second,experiment
R12927 T16362 T16359 pobj experiment,in
R12928 T16363 T16359 cc and,in
R12929 T16364 T16358 prep in,obtained
R12930 T16365 T16367 nummod two,experiments
R12931 T16366 T16367 amod additional,experiments
R12932 T16367 T16364 pobj experiments,in
R12933 T16368 T16367 prep with,experiments
R12934 T16369 T16372 det a,clone
R12935 T16370 T16372 amod second,clone
R12936 T16371 T16372 amod HOIP-deficient,clone
R12937 T16372 T16368 pobj clone,with
R12938 T16373 T16358 punct .,obtained
R13183 T16830 T16831 compound IKK,recruitment
R13184 T16831 T16831 ROOT recruitment,recruitment
R13185 T16832 T16831 prep to,recruitment
R13186 T16833 T16834 det the,CD40
R13187 T16834 T16832 pobj CD40,to
R13188 T16835 T16831 acl signaling,recruitment
R13189 T16836 T16837 nsubj complex,is
R13190 T16837 T16835 ccomp is,signaling
R13191 T16838 T16837 acomp defective,is
R13192 T16839 T16838 prep in,defective
R13193 T16840 T16841 amod HOIP-deficient,cells
R13194 T16841 T16839 pobj cells,in
R13195 T16842 T16831 punct .,recruitment
R13196 T16843 T16849 punct (,reduces
R13197 T16844 T16848 det A,treatment
R13198 T16845 T16846 punct ),SMAC
R13199 T16846 T16848 nmod SMAC,treatment
R13200 T16847 T16848 compound peptide,treatment
R13201 T16848 T16849 nsubj treatment,reduces
R13202 T16849 T16849 ROOT reduces,reduces
R13203 T16850 T16849 dobj recruitment,reduces
R13204 T16851 T16850 prep of,recruitment
R13205 T16852 T16851 pobj cIAP1,of
R13206 T16853 T16849 prep to,reduces
R13207 T16854 T16853 pobj CD40,to
R13208 T16855 T16849 cc and,reduces
R13209 T16856 T16857 aux may,modify
R13210 T16857 T16849 conj modify,reduces
R13211 T16858 T16859 compound HOIP,recruitment
R13212 T16859 T16857 dobj recruitment,modify
R13213 T16860 T16849 punct .,reduces
R13214 T16861 T16864 nummod A20,cells
R13215 T16862 T16864 nummod .2,cells
R13216 T16863 T16864 compound J,cells
R13217 T16864 T16866 nsubjpass cells,incubated
R13218 T16865 T16866 auxpass were,incubated
R13219 T16866 T16866 ROOT incubated,incubated
R13220 T16867 T16866 prep for,incubated
R13221 T16868 T16869 nummod six,hours
R13222 T16869 T16867 pobj hours,for
R13223 T16870 T16866 prep with,incubated
R13224 T16871 T16873 amod membrane-permeable,peptide
R13225 T16872 T16873 compound SMAC-N7,peptide
R13226 T16873 T16870 pobj peptide,with
R13227 T16874 T16873 cc or,peptide
R13228 T16875 T16876 nummod 1.5,%
R13229 T16876 T16877 compound %,DMSO
R13230 T16877 T16873 conj DMSO,peptide
R13231 T16878 T16880 punct (,used
R13232 T16879 T16880 advmod solvent,used
R13233 T16880 T16866 advcl used,incubated
R13234 T16881 T16880 prep for,used
R13235 T16882 T16883 det the,peptide
R13236 T16883 T16881 pobj peptide,for
R13237 T16884 T16880 punct ),used
R13238 T16885 T16866 punct .,incubated
R13239 T16886 T16893 prep Following,prepared
R13240 T16887 T16888 det the,incubation
R13241 T16888 T16886 pobj incubation,Following
R13242 T16889 T16893 punct ",",prepared
R13243 T16890 T16891 compound cell,lysates
R13244 T16891 T16893 nsubjpass lysates,prepared
R13245 T16892 T16893 auxpass were,prepared
R13246 T16893 T16893 ROOT prepared,prepared
R13247 T16894 T16893 punct ",",prepared
R13248 T16895 T16893 conj fractionated,prepared
R13249 T16896 T16895 agent by,fractionated
R13250 T16897 T16896 pobj SDS-PAGE,by
R13251 T16898 T16895 punct ",",fractionated
R13252 T16899 T16895 cc and,fractionated
R13253 T16900 T16895 conj evaluated,fractionated
R13254 T16901 T16900 agent by,evaluated
R13255 T16902 T16903 amod Western,blot
R13256 T16903 T16901 pobj blot,by
R13257 T16904 T16905 punct (,lanes
R13258 T16905 T16900 parataxis lanes,evaluated
R13259 T16906 T16905 nummod 1,lanes
R13260 T16907 T16905 cc and,lanes
R13261 T16908 T16905 conj 2,lanes
R13262 T16909 T16905 punct ),lanes
R13263 T16910 T16893 punct .,prepared
R13264 T16911 T16912 nsubj Cells,incubated
R13265 T16912 T16912 ROOT incubated,incubated
R13266 T16913 T16912 prep with,incubated
R13267 T16914 T16913 pobj DMSO,with
R13268 T16915 T16914 cc or,DMSO
R13269 T16916 T16914 conj SMAC-N7,DMSO
R13270 T16917 T16919 auxpass were,stimulated
R13271 T16918 T16919 advmod also,stimulated
R13272 T16919 T16912 conj stimulated,incubated
R13273 T16920 T16919 prep with,stimulated
R13274 T16921 T16922 amod magnetic,beads
R13275 T16922 T16920 pobj beads,with
R13276 T16923 T16922 acl coated,beads
R13277 T16924 T16923 prep with,coated
R13278 T16925 T16924 pobj anti-CD40,with
R13279 T16926 T17018 prep with,transduced
R13280 T16927 T16925 cc or,anti-CD40
R13281 T16928 T16931 det an,antibody
R13282 T16929 T16930 compound isotype,control
R13283 T16930 T16931 compound control,antibody
R13284 T16931 T16925 conj antibody,anti-CD40
R13285 T16932 T16919 punct .,stimulated
R13286 T16933 T16937 amod Immunoprecipitated,material
R13287 T16934 T16937 punct (,material
R13288 T16935 T16937 amod IP,material
R13289 T16936 T16937 punct ),material
R13290 T16937 T16943 nsubjpass material,loaded
R13291 T16938 T16937 acl bound,material
R13292 T16939 T16938 prep to,bound
R13293 T16940 T16941 det the,beads
R13294 T16941 T16939 pobj beads,to
R13295 T16942 T16943 auxpass was,loaded
R13296 T16943 T16943 ROOT loaded,loaded
R13297 T16944 T16943 prep in,loaded
R13298 T16945 T16944 pobj lanes,in
R13299 T16946 T16943 advmod 3-5,loaded
R13300 T16947 T16943 punct .,loaded
R13301 T16948 T16952 nsubj Samples,lysates
R13302 T16949 T16948 prep of,Samples
R13303 T16950 T16951 det the,cell
R13304 T16951 T16949 pobj cell,of
R13305 T16952 T16952 ROOT lysates,lysates
R13306 T16953 T16955 mark after,appear
R13307 T16954 T16955 nsubj immunoprecipitation,appear
R13308 T16955 T16952 advcl appear,lysates
R13309 T16956 T16955 prep in,appear
R13310 T16957 T16956 pobj lanes,in
R13311 T16958 T16955 npadvmod 6,appear
R13312 T16959 T16958 appos 8,6
R13313 T16960 T16952 punct .,lysates
R13314 T16961 T16962 amod Western,blots
R13315 T16962 T16964 nsubjpass blots,probed
R13316 T16963 T16964 auxpass were,probed
R13317 T16964 T16964 ROOT probed,probed
R13318 T16965 T16964 prep with,probed
R13319 T16966 T16965 pobj antibodies,with
R13320 T16967 T16966 amod specific,antibodies
R13321 T16968 T16967 prep for,specific
R13322 T16969 T16968 pobj cIAP1,for
R13323 T16970 T16969 punct ",",cIAP1
R13324 T16971 T16969 appos TRAF2,cIAP1
R13325 T16972 T16969 punct ",",cIAP1
R13326 T16973 T16969 appos TRAF3,cIAP1
R13327 T16974 T16964 punct ",",probed
R13328 T16975 T16964 cc and,probed
R13329 T16976 T16981 dep HOIP,indicated
R13330 T16977 T16981 punct (,indicated
R13331 T16978 T16980 amod approximate,weights
R13332 T16979 T16980 amod molecular,weights
R13333 T16980 T16981 nsubj weights,indicated
R13334 T16981 T16964 conj indicated,probed
R13335 T16982 T16981 prep on,indicated
R13336 T16983 T16982 pobj left,on
R13337 T16984 T16981 punct ),indicated
R13338 T16985 T16981 punct .,indicated
R13339 T16986 T16987 amod Similar,results
R13340 T16987 T16989 nsubjpass results,obtained
R13341 T16988 T16989 auxpass were,obtained
R13342 T16989 T16989 ROOT obtained,obtained
R13343 T16990 T16989 prep in,obtained
R13344 T16991 T16993 nummod two,experiments
R13345 T16992 T16993 amod additional,experiments
R13346 T16993 T16990 pobj experiments,in
R13347 T16994 T16989 punct .,obtained
R13348 T16995 T16998 punct (,CD40
R13349 T16996 T16998 nmod B,CD40
R13350 T16997 T16998 punct ),CD40
R13351 T16998 T17000 nsubjpass CD40,isolated
R13352 T16999 T17000 auxpass was,isolated
R13353 T17000 T17000 ROOT isolated,isolated
R13354 T17001 T17000 agent by,isolated
R13355 T17002 T17001 pobj immunoprecipitation,by
R13356 T17003 T17004 punct (,as
R13357 T17004 T17000 prep as,isolated
R13358 T17005 T17004 prep in,as
R13359 T17006 T17007 punct (,A
R13360 T17007 T17005 pobj A,in
R13361 T17008 T17007 punct ),A
R13362 T17009 T17004 punct ),as
R13363 T17010 T17000 prep from,isolated
R13364 T17011 T17014 nummod A20,cells
R13365 T17012 T17014 nummod .2,cells
R13366 T17013 T17014 compound J,cells
R13367 T17014 T17010 pobj cells,from
R13368 T17015 T17014 cc and,cells
R13369 T17016 T17017 amod HOIP-deficient,cells
R13370 T17017 T17014 conj cells,cells
R13371 T17018 T17000 conj transduced,isolated
R13372 T17019 T17022 det an,vector
R13373 T17020 T17022 amod empty,vector
R13374 T17021 T17022 compound retroviral,vector
R13375 T17022 T16926 pobj vector,with
R13376 T17023 T17022 punct (,vector
R13377 T17024 T17022 appos pMIP,vector
R13378 T17025 T17022 punct ),vector
R13379 T17026 T17022 cc or,vector
R13380 T17027 T17029 det a,vector
R13381 T17028 T17029 amod retroviral,vector
R13382 T17029 T17022 conj vector,vector
R13383 T17030 T17018 advcl encoding,transduced
R13384 T17031 T17030 dobj HOIP,encoding
R13385 T17032 T17000 punct .,isolated
R13386 T17033 T17034 compound Material,immunoprecipitated
R13387 T17034 T17047 nsubjpass immunoprecipitated,examined
R13388 T17035 T17034 prep with,immunoprecipitated
R13389 T17036 T17045 det an,antibody
R13390 T17037 T17038 amod isotype,control
R13391 T17038 T17045 nmod control,antibody
R13392 T17039 T17045 nmod antibody,antibody
R13393 T17040 T17041 punct (,isotype
R13394 T17041 T17045 nmod isotype,antibody
R13395 T17042 T17041 punct ),isotype
R13396 T17043 T17041 cc or,isotype
R13397 T17044 T17045 amod anti-CD40,antibody
R13398 T17045 T17035 pobj antibody,with
R13399 T17046 T17047 auxpass was,examined
R13400 T17047 T17047 ROOT examined,examined
R13401 T17048 T17047 agent by,examined
R13402 T17049 T17050 amod Western,blotting
R13403 T17050 T17048 pobj blotting,by
R13404 T17051 T17050 prep for,blotting
R13405 T17052 T17051 pobj CD40,for
R13406 T17053 T17052 punct ",",CD40
R13407 T17054 T17052 appos TRAF2,CD40
R13408 T17055 T17052 punct ",",CD40
R13409 T17056 T17052 appos TRAF3,CD40
R13410 T17057 T17052 punct ",",CD40
R13411 T17058 T17052 conj cIAP1,CD40
R13412 T17059 T17058 punct ",",cIAP1
R13413 T17060 T17058 conj HOIP,cIAP1
R13414 T17061 T17060 punct ",",HOIP
R13415 T17062 T17064 compound IKKα,β
R13416 T17063 T17064 compound /,β
R13417 T17064 T17060 conj β,HOIP
R13418 T17065 T17064 punct ",",β
R13419 T17066 T17064 cc and,β
R13420 T17067 T17064 conj IKKγ,β
R13421 T17068 T17070 punct (,panels
R13422 T17069 T17070 amod right,panels
R13423 T17070 T17067 appos panels,IKKγ
R13424 T17071 T17067 punct ),IKKγ
R13425 T17072 T17047 punct .,examined
R13426 T17073 T17074 compound HOIP,expression
R13427 T17074 T17076 nsubjpass expression,required
R13428 T17075 T17076 auxpass was,required
R13429 T17076 T17076 ROOT required,required
R13430 T17077 T17076 prep for,required
R13431 T17078 T17077 pobj coprecipitation,for
R13432 T17079 T17078 prep of,coprecipitation
R13433 T17080 T17081 compound IKK,proteins
R13434 T17081 T17079 pobj proteins,of
R13435 T17082 T17076 prep with,required
R13436 T17083 T17082 pobj CD40,with
R13437 T17084 T17076 punct .,required
R13438 T17085 T17086 nsubj Cell,lysates
R13439 T17086 T17086 ROOT lysates,lysates
R13440 T17087 T17086 prep from,lysates
R13441 T17088 T17089 amod unstimulated,cells
R13442 T17089 T17087 pobj cells,from
R13443 T17090 T17091 auxpass are,shown
R13444 T17091 T17086 conj shown,lysates
R13445 T17092 T17091 prep in,shown
R13446 T17093 T17095 det the,panels
R13447 T17094 T17095 amod left,panels
R13448 T17095 T17092 pobj panels,in
R13449 T17096 T17086 punct .,lysates
R13450 T17097 T17098 amod Similar,results
R13451 T17098 T17100 nsubjpass results,obtained
R13452 T17099 T17100 auxpass were,obtained
R13453 T17100 T17100 ROOT obtained,obtained
R13454 T17101 T17100 prep in,obtained
R13455 T17102 T17104 det a,experiment
R13456 T17103 T17104 amod second,experiment
R13457 T17104 T17101 pobj experiment,in
R13458 T17105 T17101 cc and,in
R13459 T17106 T17101 conj in,in
R13460 T17107 T17108 nummod two,experiments
R13461 T17108 T17106 pobj experiments,in
R13462 T17109 T17108 prep with,experiments
R13463 T17110 T17113 det a,clone
R13464 T17111 T17113 amod second,clone
R13465 T17112 T17113 amod HOIP-deficient,clone
R13466 T17113 T17109 pobj clone,with
R13467 T17114 T17100 punct .,obtained
R13468 T17115 T17116 punct (,C
R13469 T17116 T17116 ROOT C,C
R13470 T17117 T17116 punct ),C
R13471 T17118 T17200 pobj blotting,by
R13472 T17119 T17121 aux To,evaluate
R13473 T17120 T17121 advmod further,evaluate
R13474 T17121 T17138 advcl evaluate,/
R13475 T17122 T17123 amod HOIP-dependent,recruitment
R13476 T17123 T17121 dobj recruitment,evaluate
R13477 T17124 T17123 prep of,recruitment
R13478 T17125 T17124 pobj IKKγ,of
R13479 T17126 T17121 prep to,evaluate
R13480 T17127 T17126 pobj CD40,to
R13481 T17128 T17127 punct ",",CD40
R13482 T17129 T17138 ccomp A20,/
R13483 T17130 T17132 nummod .2,cells
R13484 T17131 T17132 compound J,cells
R13485 T17132 T17129 dobj cells,A20
R13486 T17133 T17132 cc or,cells
R13487 T17134 T17132 conj HOIP-deficient,cells
R13488 T17135 T17134 punct (,HOIP-deficient
R13489 T17136 T17138 punct HOIP,/
R13490 T17137 T17138 punct -,/
R13491 T17138 T17138 ROOT /,/
R13492 T17139 T17138 punct -,/
R13493 T17140 T17138 punct ),/
R13494 T17141 T17144 nummod A20,cells
R13495 T17142 T17144 nummod .2,cells
R13496 T17143 T17144 compound J,cells
R13497 T17144 T17146 nsubjpass cells,transduced
R13498 T17145 T17146 auxpass were,transduced
R13499 T17146 T17146 ROOT transduced,transduced
R13500 T17147 T17146 prep with,transduced
R13501 T17148 T17151 det an,vector
R13502 T17149 T17151 amod empty,vector
R13503 T17150 T17151 compound retroviral,vector
R13504 T17151 T17147 pobj vector,with
R13505 T17152 T17151 cc or,vector
R13506 T17153 T17155 det a,construct
R13507 T17154 T17155 amod retroviral,construct
R13508 T17155 T17146 advcl construct,transduced
R13509 T17156 T17155 xcomp encoding,construct
R13510 T17157 T17159 compound BP,IKKγ
R13511 T17158 T17159 compound epitope-tagged,IKKγ
R13512 T17159 T17156 dobj IKKγ,encoding
R13513 T17160 T17161 punct (,noted
R13514 T17161 T17156 advcl noted,encoding
R13515 T17162 T17161 prep in,noted
R13516 T17163 T17164 det the,figure
R13517 T17164 T17162 pobj figure,in
R13518 T17165 T17164 prep as,figure
R13519 T17166 T17165 pobj pMIP,as
R13520 T17167 T17166 cc and,pMIP
R13521 T17168 T17166 conj IKKγ,pMIP
R13522 T17169 T17168 punct ",",IKKγ
R13523 T17170 T17168 conj respectively,IKKγ
R13524 T17171 T17161 punct ),noted
R13525 T17172 T17146 punct .,transduced
R13526 T17173 T17173 ROOT Lysates,Lysates
R13527 T17174 T17175 punct (,lanes
R13528 T17175 T17175 ROOT lanes,lanes
R13529 T17176 T17175 nummod 1,lanes
R13530 T17177 T17184 nmod 3,samples
R13531 T17178 T17177 punct ),3
R13532 T17179 T17177 cc and,3
R13533 T17180 T17177 conj immunoprecipitation,3
R13534 T17181 T17182 punct (,IP
R13535 T17182 T17180 appos IP,immunoprecipitation
R13536 T17183 T17180 punct ),immunoprecipitation
R13537 T17184 T17195 nsubjpass samples,fractionated
R13538 T17185 T17186 punct (,lanes
R13539 T17186 T17184 parataxis lanes,samples
R13540 T17187 T17186 nummod 4,lanes
R13541 T17188 T17186 appos 11,lanes
R13542 T17189 T17186 punct ),lanes
R13543 T17190 T17184 prep from,samples
R13544 T17191 T17193 det the,lines
R13545 T17192 T17193 compound cell,lines
R13546 T17193 T17190 pobj lines,from
R13547 T17194 T17195 auxpass were,fractionated
R13548 T17195 T17195 ROOT fractionated,fractionated
R13549 T17196 T17195 agent by,fractionated
R13550 T17197 T17196 pobj SDS-PAGE,by
R13551 T17198 T17195 cc and,fractionated
R13552 T17199 T17195 conj evaluated,fractionated
R13553 T17200 T17199 agent by,evaluated
R13554 T17201 T17118 amod Western,blotting
R13555 T17202 T17118 prep with,blotting
R13556 T17203 T17202 pobj antibodies,with
R13557 T17204 T17199 prep to,evaluated
R13558 T17205 T17207 det the,tag
R13559 T17206 T17207 compound BP,tag
R13560 T17207 T17204 pobj tag,to
R13561 T17208 T17209 punct (,IKKγ
R13562 T17209 T17207 appos IKKγ,tag
R13563 T17210 T17209 punct ",",IKKγ
R13564 T17211 T17212 amod upper,panel
R13565 T17212 T17209 appos panel,IKKγ
R13566 T17213 T17209 punct ),IKKγ
R13567 T17214 T17207 cc and,tag
R13568 T17215 T17207 conj TRAF2,tag
R13569 T17216 T17195 punct .,fractionated
R13570 T17217 T17220 det The,sample
R13571 T17218 T17220 amod anti-CD40,sample
R13572 T17219 T17220 amod IP,sample
R13573 T17220 T17225 nsubjpass sample,treated
R13574 T17221 T17220 prep in,sample
R13575 T17222 T17221 pobj lane,in
R13576 T17223 T17222 nummod 11,lane
R13577 T17224 T17225 auxpass was,treated
R13578 T17225 T17235 ccomp treated,was
R13579 T17226 T17225 prep with,treated
R13580 T17227 T17228 amod λ,phosphatase
R13581 T17228 T17226 pobj phosphatase,with
R13582 T17229 T17235 punct ;,was
R13583 T17230 T17231 det the,sample
R13584 T17231 T17235 nsubj sample,was
R13585 T17232 T17231 prep in,sample
R13586 T17233 T17232 pobj lane,in
R13587 T17234 T17233 nummod 10,lane
R13588 T17235 T17235 ROOT was,was
R13589 T17236 T17235 attr mock-treated,was
R13590 T17237 T17235 punct .,was
R13591 T17238 T17239 compound Protein,samples
R13592 T17239 T17249 nsubjpass samples,fractionated
R13593 T17240 T17239 punct (,samples
R13594 T17241 T17242 acomp minus,those
R13595 T17242 T17239 appos those,samples
R13596 T17243 T17242 acl treated,those
R13597 T17244 T17243 prep with,treated
R13598 T17245 T17244 pobj phosphatase,with
R13599 T17246 T17239 punct ),samples
R13600 T17247 T17249 auxpass were,fractionated
R13601 T17248 T17249 advmod also,fractionated
R13602 T17249 T17249 ROOT fractionated,fractionated
R13603 T17250 T17249 prep on,fractionated
R13604 T17251 T17253 det a,gel
R13605 T17252 T17253 amod separate,gel
R13606 T17253 T17250 pobj gel,on
R13607 T17254 T17253 punct (,gel
R13608 T17255 T17257 amod lower,concentration
R13609 T17256 T17257 compound acrylamide,concentration
R13610 T17257 T17253 appos concentration,gel
R13611 T17258 T17253 punct ),gel
R13612 T17259 T17249 prep for,fractionated
R13613 T17260 T17261 det the,evaluation
R13614 T17261 T17259 pobj evaluation,for
R13615 T17262 T17261 prep of,evaluation
R13616 T17263 T17262 pobj TRAF3,of
R13617 T17264 T17263 cc and,TRAF3
R13618 T17265 T17263 conj HOIP,TRAF3
R13619 T17266 T17269 punct (,panels
R13620 T17267 T17269 amod bottom,panels
R13621 T17268 T17269 nummod two,panels
R13622 T17269 T17265 appos panels,HOIP
R13623 T17270 T17265 punct ),HOIP
R13624 T17271 T17249 punct .,fractionated
R13625 T17272 T17273 amod Similar,results
R13626 T17273 T17275 nsubjpass results,obtained
R13627 T17274 T17275 auxpass were,obtained
R13628 T17275 T17275 ROOT obtained,obtained
R13629 T17276 T17275 prep in,obtained
R13630 T17277 T17279 nummod two,experiments
R13631 T17278 T17279 amod additional,experiments
R13632 T17279 T17276 pobj experiments,in
R13633 T17280 T17275 punct .,obtained

GO-BP

Id Subject Object Predicate Lexical cue
T3835 177-186 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T3836 735-744 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T3837 925-934 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T3838 3394-3403 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T3839 643-662 http://purl.obolibrary.org/obo/GO_0007165 denotes signal transduction
T3840 650-662 http://purl.obolibrary.org/obo/GO_0009293 denotes transduction
T5069 4001-4014 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T5070 6268-6281 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T5071 4084-4104 http://purl.obolibrary.org/obo/GO_0033580 denotes cell surface protein
T5072 4084-4104 http://purl.obolibrary.org/obo/GO_0034394 denotes cell surface protein
T5073 4084-4104 http://purl.obolibrary.org/obo/GO_0033575 denotes cell surface protein
T5074 4241-4250 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T5075 6710-6719 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T5076 5388-5419 http://purl.obolibrary.org/obo/GO_0045911 denotes activation of DNA recombination
T5077 5402-5419 http://purl.obolibrary.org/obo/GO_0006310 denotes DNA recombination
T5078 5510-5525 http://purl.obolibrary.org/obo/GO_0045190 denotes class switching
T5079 5516-5537 http://purl.obolibrary.org/obo/GO_0045190 denotes switching (or isotype
T5080 5510-5537 http://purl.obolibrary.org/obo/GO_0048290 denotes class switching (or isotype
T5081 5510-5537 http://purl.obolibrary.org/obo/GO_0048292 denotes class switching (or isotype
T5082 5510-5537 http://purl.obolibrary.org/obo/GO_0048289 denotes class switching (or isotype
T5083 5510-5537 http://purl.obolibrary.org/obo/GO_0048291 denotes class switching (or isotype
T5084 5691-5706 http://purl.obolibrary.org/obo/GO_0006955 denotes immune response
T5085 6683-6691 http://purl.obolibrary.org/obo/GO_0007349 denotes cellular
T6097 7624-7627 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T6098 7995-7998 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T6099 8883-8886 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T6100 8942-8945 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T6101 9049-9052 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T6102 9091-9094 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T6103 7808-7817 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T6104 8230-8237 http://purl.obolibrary.org/obo/GO_0023052 denotes signals
T6105 7967-7990 http://purl.obolibrary.org/obo/GO_0051092 denotes activation of the NF-κB
T6106 9067-9086 http://purl.obolibrary.org/obo/GO_0051092 denotes activation of NF-κB
T6107 8026-8041 http://purl.obolibrary.org/obo/GO_0006351 denotes transcriptional
T6108 8332-8347 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T6109 8666-8681 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T6110 9009-9024 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T6111 8332-8354 http://purl.obolibrary.org/obo/GO_0007252 denotes phosphorylation of IκB
T6112 8391-8394 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK
T6113 8517-8520 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK
T6114 8448-8460 http://purl.obolibrary.org/obo/GO_0051235 denotes sequestering
T6115 8541-8568 http://purl.obolibrary.org/obo/GO_0016567 denotes proteins for ubiquitination
T6116 8573-8584 http://purl.obolibrary.org/obo/GO_0009056 denotes degradation
T6117 8686-8697 http://purl.obolibrary.org/obo/GO_0009056 denotes degradation
T6118 8635-8650 http://purl.obolibrary.org/obo/GO_0010467 denotes gene expression
T6119 8858-8882 http://purl.obolibrary.org/obo/GO_0034199 denotes activated protein kinase
T6120 8858-8882 http://purl.obolibrary.org/obo/GO_0032147 denotes activated protein kinase
T6121 8858-8882 http://purl.obolibrary.org/obo/GO_0030295 denotes activated protein kinase
T6122 8858-8882 http://purl.obolibrary.org/obo/GO_0032111 denotes activated protein kinase
T8280 15400-15403 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK
T8281 10409-10424 http://purl.obolibrary.org/obo/GO_0001775 denotes cell activation
T8282 10429-10438 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T8283 10549-10558 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T8284 10649-10658 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T8285 11246-11255 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T8286 14569-14578 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T8287 14624-14633 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T8288 14734-14743 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T8289 15587-15596 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T8290 11091-11100 http://purl.obolibrary.org/obo/GO_0006915 denotes apoptosis
T8291 11091-11100 http://purl.obolibrary.org/obo/GO_0097194 denotes apoptosis
T8292 11841-11849 http://purl.obolibrary.org/obo/GO_0007349 denotes cellular
T8293 11966-11997 http://purl.obolibrary.org/obo/GO_0043687 denotes post-translational modification
T8294 17140-17172 http://purl.obolibrary.org/obo/GO_0043687 denotes post-translational modifications
T8295 11966-11997 http://purl.obolibrary.org/obo/GO_1901873 denotes post-translational modification
T8296 17140-17172 http://purl.obolibrary.org/obo/GO_1901873 denotes post-translational modifications
T8297 11966-11997 http://purl.obolibrary.org/obo/GO_1901874 denotes post-translational modification
T8298 17140-17172 http://purl.obolibrary.org/obo/GO_1901874 denotes post-translational modifications
T8299 11966-11997 http://purl.obolibrary.org/obo/GO_1901875 denotes post-translational modification
T8300 17140-17172 http://purl.obolibrary.org/obo/GO_1901875 denotes post-translational modifications
T8301 16539-16550 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T8302 16867-16878 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T8303 16988-17003 http://purl.obolibrary.org/obo/GO_0016310 denotes phosphorylation
T15254 2883-2898 http://purl.obolibrary.org/obo/GO_0010467 denotes gene expression
T15595 5097-5109 http://purl.obolibrary.org/obo/GO_0009293 denotes Transduction
T15596 5231-5243 http://purl.obolibrary.org/obo/GO_0009293 denotes transduction
T16041 6769-6782 http://purl.obolibrary.org/obo/GO_0006351 denotes transcription
T16565 9300-9319 http://purl.obolibrary.org/obo/GO_0051092 denotes activation of NF-κB
T16566 9324-9327 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T16567 9928-9931 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T16568 9997-10000 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T16569 10021-10024 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T16570 9380-9389 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T17748 12073-12076 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK
T17749 13425-13428 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK
T17750 12101-12110 http://purl.obolibrary.org/obo/GO_0023052 denotes signaling
T17751 14179-14190 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T17752 14274-14285 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase

GO-MF

Id Subject Object Predicate Lexical cue
T5086 5427-5441 http://purl.obolibrary.org/obo/GO_0003823 denotes immunoglobulin
T5087 5495-5509 http://purl.obolibrary.org/obo/GO_0003823 denotes immunoglobulin
T5088 5648-5656 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T5089 6207-6215 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T5090 6438-6446 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T5091 6575-6583 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T5092 5762-5766 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T5093 6118-6122 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T5094 6251-6255 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T5095 6470-6474 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T5096 6607-6611 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T6123 7624-7627 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T6124 7995-7998 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T6125 8883-8886 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T6126 8942-8945 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T6127 9049-9052 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T6128 9091-9094 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T6129 7907-7913 http://purl.obolibrary.org/obo/GO_0005488 denotes ligand
T6130 8258-8266 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T6131 8391-8394 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK
T6132 8517-8520 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK
T6133 8858-8882 http://purl.obolibrary.org/obo/GO_0030295 denotes activated protein kinase
T8305 15400-15403 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK
T8306 11299-11307 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T8307 11574-11582 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T8308 16200-16208 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T8309 16539-16550 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T8310 16867-16878 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T15597 4889-4897 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T15598 4918-4926 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T15599 5025-5033 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T15600 5086-5094 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T16042 7117-7125 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T16043 7149-7153 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4
T16571 9324-9327 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T16572 9928-9931 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T16573 9997-10000 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T16574 10021-10024 http://purl.obolibrary.org/obo/GO_0004705 denotes JNK
T16575 9883-9893 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T16576 10025-10035 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T17753 12073-12076 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK
T17754 13425-13428 http://purl.obolibrary.org/obo/GO_0008384 denotes IKK
T17755 12630-12638 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T17756 13229-13237 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T17757 13261-13269 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T17758 14069-14079 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies
T17759 14179-14190 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase
T17760 14274-14285 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase

GO-CC

Id Subject Object Predicate Lexical cue
T6145 8258-8266 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T6146 8382-8394 http://purl.obolibrary.org/obo/GO_0008385 denotes complex (IKK
T6147 8517-8528 http://purl.obolibrary.org/obo/GO_0008385 denotes IKK complex
T6148 8483-8492 http://purl.obolibrary.org/obo/GO_0005737 denotes cytoplasm
T6149 8614-8621 http://purl.obolibrary.org/obo/GO_0005634 denotes nucleus
T15257 2469-2480 http://purl.obolibrary.org/obo/GO_0005694 denotes chromosomal
T15258 2708-2719 http://purl.obolibrary.org/obo/GO_0005694 denotes chromosomal
T15259 2686-2691 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15260 3006-3011 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15261 3109-3114 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16044 6830-6835 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16045 6863-6868 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16046 6980-6985 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16047 7181-7186 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16048 7117-7125 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T16049 7117-7125 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T16577 9359-9364 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16578 9422-9427 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16579 9469-9474 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16580 9556-9561 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16581 9680-9685 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16582 9734-9739 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16583 9768-9773 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16584 9839-9843 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T16585 9883-9893 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T16586 10025-10035 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T16587 9883-9893 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T16588 10025-10035 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T17777 14069-14079 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies
T6134 7747-7752 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6135 7884-7889 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6136 7939-7944 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T17761 12150-12155 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T17762 12261-12266 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T17763 13058-13063 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T17764 13083-13088 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T17765 13480-13485 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T17766 13703-13708 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T17767 13744-13749 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T17768 13989-13993 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T17769 12301-12309 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T17770 12630-12638 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T17771 13229-13237 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T17772 13261-13269 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T17773 14069-14079 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T17774 12630-12638 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T17775 13229-13237 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T17776 13261-13269 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T6137 8408-8413 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6138 8724-8729 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6139 8801-8806 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6140 8975-8980 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6141 9117-9122 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T6142 9193-9198 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3841 40-45 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3842 1367-1372 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3843 1677-1682 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3844 2074-2079 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3845 3453-3458 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3846 3659-3664 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3847 3703-3708 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3848 3761-3766 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3849 3800-3805 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T3850 3825-3830 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5097 4039-4044 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5098 4135-4140 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5099 4160-4165 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5100 4377-4382 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5101 4517-4522 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5102 4600-4605 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5103 4677-4682 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5104 5355-5360 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5105 5559-5564 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5106 6301-6306 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5107 6399-6404 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5108 6543-6548 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5109 4084-4096 http://purl.obolibrary.org/obo/GO_0009986 denotes cell surface
T5110 4176-4179 http://purl.obolibrary.org/obo/GO_0005680 denotes APC
T6143 8076-8091 http://purl.obolibrary.org/obo/GO_0009986 denotes Cell-associated
T6144 8258-8266 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T5111 5648-5656 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T5112 6207-6215 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T5113 6438-6446 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T5114 6575-6583 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T5115 5648-5656 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T5116 6207-6215 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T5117 6438-6446 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T5118 6575-6583 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T8312 10409-10413 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T8313 11693-11697 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T8314 15752-15756 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T8315 15856-15860 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T8316 16760-16764 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T8317 11047-11055 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T8318 11299-11307 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T8319 11574-11582 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T8320 16200-16208 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T8321 11299-11307 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T8322 11574-11582 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T8323 16200-16208 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T15601 4826-4831 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T15602 4889-4897 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T15603 4918-4926 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T15604 5025-5033 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T15605 5086-5094 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T15606 4889-4897 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T15607 4918-4926 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T15608 5025-5033 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T15609 5086-5094 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody

sentences

Id Subject Object Predicate Lexical cue
T2863 9-102 Sentence denotes Generation of HOIP-deficient B cells via targeted disruption of Rnf31, the gene encoding HOIP
T2864 103-225 Sentence denotes In our previous study, we demonstrated that HOIP is recruited to the CD40 signaling complex in two mouse B cell lines [6].
T2865 226-400 Sentence denotes Similar results were obtained with mouse splenocytes, indicating that the interaction of CD40 with HOIP is not limited to transformed B cell lines (unpublished observations).
T2866 401-574 Sentence denotes We also found that the recruitment of HOIP to CD40 was TRAF2-dependent and that overexpression of a truncated HOIP mutant partially inhibited CD40-mediated NF-κB activation.
T2867 575-663 Sentence denotes These results support the hypothesis that HOIP plays a role in CD40 signal transduction.
T2868 664-848 Sentence denotes To further define the role and evaluate the importance of HOIP in CD40 signaling, we used somatic cell gene targeting to disrupt the gene encoding HOIP in the mouse B cell line A20.2J.
T2869 849-1051 Sentence denotes This cell line has been particularly useful in the characterization of CD40 signaling mechanisms due to the relative ease with which its genome can be modified by homologous recombination [7], [8], [9].
T2870 1052-1225 Sentence denotes We used a targeting vector capable of undergoing homologous recombination with Rnf31 (the gene encoding HOIP) to disrupt the coding sequence of the gene in exon 5 (Fig. 1A).
T2871 1226-1409 Sentence denotes Following introduction of the vector, the neomycin-resistant clones that arose were screened by PCR amplification of genomic DNA to identify cells containing a disrupted Rnf31 allele.
T2872 1410-1585 Sentence denotes To remove the selectable marker gene cassette from the disrupted Rnf31 allele, recombinant cell lines were transiently transfected with a plasmid that encodes Cre recombinase.
T2873 1586-1728 Sentence denotes This step allowed us to perform a second round of targeting and drug selection, generating cells in which both copies of Rnf31 were disrupted.
T2874 1729-1796 Sentence denotes Two independent clonal cell lines were chosen for further analysis.
T2875 1797-1980 Sentence denotes HOIP protein expression was undetectable in both cell lines, as determined by Western blot analysis of cell lysates (Fig. 1B), demonstrating that the targeting process was successful.
T2876 1981-2080 Sentence denotes In the text that follows, the two gene-targeted cell lines are referred to as HOIP-deficient cells.
T2877 3361-3652 Sentence denotes To enable us to confirm that any signaling or functional defects observed in HOIP-deficient cells were due to disruption of the Rnf31 gene, both HOIP-deficient cell lines were transduced with a retrovirus encoding FLAG-tagged wild-type HOIP, thus restoring HOIP protein expression (Fig. 1B).
T2878 3653-3709 Sentence denotes These cells are referred to as HOIP-reconstituted cells.
T2879 3710-3911 Sentence denotes In the experiments that follow, responses by these cells were compared to those of A20.2J cells and HOIP-deficient cells transduced with a retroviral vector (pMIP) lacking a cDNA insert (empty vector).
T4245 3913-4014 Sentence denotes HOIP is required for CD40-mediated CD80 upregulation and activation of germline epsilon transcription
T4246 4015-4180 Sentence denotes Engagement of CD40 on B cells upregulates expression of CD80 [10], a cell surface protein that promotes activation of T cells interacting with B cells and other APC.
T4247 4181-4392 Sentence denotes To begin evaluating potential contributions of HOIP to CD40 signaling, we assayed the upregulation of CD80 induced by engagement of CD40 on parental A20.2J, HOIP-deficient, and HOIP-reconstituted cells (Fig. 2).
T4248 4393-4530 Sentence denotes Compared to the parental cell line, the CD40-stimulated upregulation of CD80 was dramatically reduced in all HOIP-deficient cells tested.
T4249 4531-4718 Sentence denotes In contrast, upregulation of CD80 was restored in HOIP-reconstituted cells, confirming that the defect in CD80 upregulation in the HOIP-deficient cells was due to the disruption of Rnf31.
T4250 5337-5471 Sentence denotes CD40 signals in B cells can also contribute to the activation of DNA recombination in the immunoglobulin heavy chain locus [11], [12].
T4251 5472-5707 Sentence denotes This process, known as immunoglobulin class switching (or isotype switching), allows B cells, initially expressing IgM, to switch to IgA, IgG, or IgE depending on the class of antibody most appropriate for a particular immune response.
T4252 5708-5784 Sentence denotes Switching to IgE can be induced by the combination of IL-4 and CD40 signals.
T4253 5785-5955 Sentence denotes The gene rearrangement necessary for IgE production is preceded by the production of non-coding RNA transcripts from germline sequences in the IgE heavy chain locus [13].
T4254 5956-6196 Sentence denotes To determine whether HOIP is required in the initiation of the class switching process, the relevant B cell lines were stimulated through CD40 in the presence of IL-4 and tested for production of germline epsilon (GLε) transcripts (Fig. 3).
T4255 6197-6319 Sentence denotes Anti-CD40 antibody stimulation alone or together with IL-4 induced GLε transcription in parental A20.2J cells as expected.
T4256 6320-6475 Sentence denotes In contrast, only low levels of GLε expression were detected in HOIP-deficient cells stimulated with either anti-CD40 antibody alone or anti-CD40 and IL-4.
T4257 6476-6612 Sentence denotes Normal levels of GLε expression were induced in HOIP-reconstituted cells stimulated with anti-CD40 antibody alone or together with IL-4.
T4258 6613-6720 Sentence denotes Collectively, these data demonstrate that HOIP has a critical role in cellular responses to CD40 signaling.
T5496 7584-7638 Sentence denotes HOIP mediates CD40-stimulated NF-κB and JNK activation
T5497 7639-7753 Sentence denotes The results described above show that HOIP plays an important role in CD40-mediated effector functions of B cells.
T5498 7754-7818 Sentence denotes It follows that HOIP is likely a key mediator of CD40 signaling.
T5499 7819-8075 Sentence denotes To test this hypothesis, we stimulated A20.2J and HOIP-deficient cells with CD154 (CD40 ligand) expressed by HI5 insect cells [7], [8] and measured activation of the NF-κB and JNK pathways, two of the major transcriptional regulators activated by CD40 [4].
T5500 8076-8267 Sentence denotes Cell-associated CD154 was used as the stimulus in these experiments as it typically provides more robust, and therefore more readily detected, activation signals than does anti-CD40 antibody.
T5501 8268-8396 Sentence denotes Activation of the canonical NF-κB pathway is initiated with the phosphorylation of IκB proteins by the IκB kinase complex (IKK).
T5502 8397-8493 Sentence denotes In resting cells, IκB proteins are responsible for sequestering NF-κB subunits in the cytoplasm.
T5503 8494-8651 Sentence denotes Phosphorylation by the IKK complex targets IκB proteins for ubiquitination and degradation, allowing NF-κB to enter the nucleus and activate gene expression.
T5504 8652-8816 Sentence denotes CD40-mediated phosphorylation and degradation of IκBα in HOIP-deficient cells was dramatically impaired relative to that observed in parental A20.2J cells (Fig. 4).
T5505 8817-8927 Sentence denotes We also assayed activation of the stress-activated protein kinase JNK in response to CD40 engagement (Fig. 4).
T5506 8928-9053 Sentence denotes CD40-mediated JNK activation in HOIP-deficient cells was impaired as measured by phosphorylation of Thr183 and Tyr185 in JNK.
T5507 9054-9242 Sentence denotes CD40-induced activation of NF-κB and JNK in HOIP-reconstituted cells was normal, demonstrating that the defects observed in gene-deficient cells were due to the absence of HOIP expression.
T6706 10373-10585 Sentence denotes The marked defects in CD40-mediated cell activation and signaling displayed by HOIP-deficient cells suggested that HOIP mediates recruitment of critical components of the CD40 signaling apparatus to the receptor.
T6707 10586-10751 Sentence denotes Previously, we demonstrated that HOIP is recruited to the CD40 signaling complex in a TRAF2-dependent manner, suggesting that HOIP functions downstream of TRAF2 [6].
T6708 10752-10891 Sentence denotes Studies by others suggest that the TRAF2-associated proteins cIAP1 and cIAP2 play a role in the recruitment of HOIP to TNFR1 and CD40 [14].
T6709 10892-11121 Sentence denotes Therefore, we determined whether the association of HOIP with CD40 in A20.2J cells was altered by treatment of cells with an inhibitor of cIAP activity, a membrane-permeable peptide derived from the apoptosis regulator SMAC [15].
T6710 11122-11331 Sentence denotes We found that pretreatment of cells with the SMAC peptide dramatically reduced the amount of cIAP1 associated with the CD40 signaling complex in cells stimulated with anti-CD40 antibody-coated beads (Fig. 5A).
T6711 11332-11583 Sentence denotes SMAC peptide treatment also resulted in a slight but reproducible decrease in the amount of the major HOIP form recovered by CD40 immunoprecipitation, along with an apparent increase in higher molecular weight species recognized by anti-HOIP antibody.
T6712 11584-11706 Sentence denotes In contrast, treatment with the SMAC peptide did not alter the amount or molecular weight of HOIP present in cell lysates.
T6713 11707-11871 Sentence denotes These data suggest that SMAC peptide treatment specifically alters the characteristics of CD40-associated HOIP rather than the entire cellular pool of this protein.
T6714 11872-12028 Sentence denotes Together, these results support the idea that the cIAP proteins influence the recruitment and post-translational modification state of CD40-associated HOIP.
T6715 14482-14703 Sentence denotes To test the possibility that HOIP is responsible for the recruitment of other critical signaling proteins to CD40, we immunoprecipitated CD40 signaling complexes from HOIP-deficient and HOIP-reconstituted cells (Fig. 5B).
T6716 14704-14852 Sentence denotes As described previously, CD40 signaling complexes immunoprecipitated from A20.2J cells contained HOIP as well as TRAF2, TRAF3, IKKα/β, and IKKγ [6].
T6717 14853-15080 Sentence denotes The amounts of TRAF2, TRAF3, and cIAP1 in CD40 immunoprecipitates from HOIP-deficient cells were similar to those from parental A20.2J cells, indicating that HOIP is not required for the association of these proteins with CD40.
T6718 15081-15205 Sentence denotes In contrast, IKKα/β and IKKγ were not detectable in CD40 immunoprecipitates recovered from HOIP-deficient cells (Fig. 5B,C).
T6719 15206-15484 Sentence denotes The amounts of IKKα/β and IKKγ in CD40 immunoprecipitates from HOIP-reconstituted cells were similar to those detected in samples prepared from parental cells, demonstrating that the defects in IKK recruitment we observed were due specifically to the absence of HOIP expression.
T6720 15485-15605 Sentence denotes These data indicate that HOIP is required for recruitment of the NF-κB-activating complex to the CD40 signaling complex.
T6721 15606-15765 Sentence denotes As shown here and in our previous study [6], IKKγ present in CD40 immunoprecipitates appears to have a higher molecular weight than that found in cell lysates.
T6722 15766-15969 Sentence denotes To confirm that this higher molecular weight species was indeed IKKγ, we generated A20.2J cell lines containing a stably integrated retroviral vector that encoded an epitope-tagged version of mouse IKKγ.
T6723 15970-16101 Sentence denotes Cell lysates and CD40 immunoprecipitates prepared from these cells were analyzed by Western blotting for the epitope tag (Fig. 5C).
T6724 16102-16269 Sentence denotes This analysis produced a pattern of bands that was essentially the same as that obtained using an antibody specific for native IKKγ (compare panels B and C in Fig. 5).
T6725 16270-16386 Sentence denotes Moreover, analysis of cells expressing epitope tagged-IKKγ confirmed that recruitment of IKKγ to CD40 requires HOIP.
T6726 16387-16641 Sentence denotes To determine what was responsible for the increased molecular weight of IKKγ in CD40 immunoprecipitates, the protein samples were incubated with lambda phosphatase, which removes phosphates attached to tyrosine, threonine, or serine residues in proteins.
T6727 16642-16783 Sentence denotes This treatment reduced much of CD40-associated IKKγ to an apparent molecular weight similar to that of the protein in cell lysates (Fig. 5C).
T6728 16784-17004 Sentence denotes However, at least one band of significantly higher molecular weight remained after phosphatase treatment, suggesting that CD40-associated IKKγ is subject to at least one other modification in addition to phosphorylation.
T6729 17005-17181 Sentence denotes Overall, these data demonstrate that HOIP is required for the association of IKKγ with CD40, and suggest that this event is coupled to post-translational modifications of IKKγ.
T14797 2125-2145 Sentence denotes HOIP gene targeting.
T14798 2146-2245 Sentence denotes (A) Regions of Rnf31 gene sequence (bottom) used in the HOIP gene targeting vector (top) are shown.
T14799 2246-2398 Sentence denotes Arrows (F and R) indicate approximate positions of sequences homologous to oligonucleotides used for PCR-mediated detection of homologous recombination.
T14800 2399-2600 Sentence denotes Homologous recombination of the vector with Rnf31 resulted in a small chromosomal deletion and the in-frame insertion of neomycin phosphotransferase (NeoR) into the amino-terminal HOIP coding sequence.
T14801 2601-2756 Sentence denotes A diphtheria toxin (DT) cassette in the vector facilitates the negative selection of cells in which random chromosomal insertion of the vector takes place.
T14802 2757-2832 Sentence denotes LoxP sequences allow the Cre-mediated deletion of the NeoR coding sequence.
T14803 2833-3048 Sentence denotes The SV40pA sequence helps to ensure disruption of gene expression after deletion of the NeoR sequence. (B) Anti-HOIP and anti-FLAG Western blots of cell lysates from A20.2J cells and HOIP-deficient (HOIP-/-) clones.
T14804 3049-3167 Sentence denotes A partial decrease in HOIP protein expression is evident in cells following disruption of one copy of Rnf31 (HOIP−/+).
T14805 3168-3310 Sentence denotes HOIP expression in clones reconstituted with an empty retroviral vector (pMIP) or a retroviral vector encoding FLAG-tagged HOIP is also shown.
T14806 3311-3358 Sentence denotes Approximate molecular weight of HOIP is 120 kD.
T15388 4763-4832 Sentence denotes CD40-mediated CD80 upregulation is defective in HOIP-deficient cells.
T15389 4833-5096 Sentence denotes Cells were incubated for 72 hrs with an isotype control antibody (left) or anti-CD40 antibody (right) and then stained for expression of CD80 (filled profiles indicate staining with anti-CD80 antibody; open profiles are staining with an isotype control antibody).
T15390 5097-5280 Sentence denotes Transduction of both HOIP-deficient (HOIP-/-) clones with a retrovirus encoding FLAG-HOIP reversed defective CD80 upregulation, while transduction with an empty vector (pMIP) did not.
T15391 5281-5334 Sentence denotes Similar results were obtained in a second experiment.
T15736 6765-6836 Sentence denotes GLε transcription is defective in CD40-stimulated HOIP-deficient cells.
T15737 6837-7154 Sentence denotes A20.2J and HOIP-deficient cells transduced with an empty retroviral vector (A20.2J + pMIP and HOIP-/- + pMIP, respectively), or HOIP-deficient cells transduced with a HOIP-encoding retrovirus (HOIP-/- + HOIP) were cultured overnight with agonistic anti-CD40 or an isotype control antibody (iso), with or without IL-4.
T15738 7155-7287 Sentence denotes RNA was isolated from the cells, reverse-transcribed, and then subjected to quantitative PCR to determine levels of GLε transcripts.
T15739 7288-7362 Sentence denotes Results were normalized to the levels of Hprt1 transcripts in each sample.
T15740 7363-7461 Sentence denotes Symbols indicate the values from duplicate cultures (a line indicates the mean of the two values).
T15741 7462-7582 Sentence denotes Similar results were obtained in a second experiment and in an additional experiment with a second HOIP-deficient clone.
T16183 9287-9365 Sentence denotes CD40-induced activation of NF-κB and JNK is defective in HOIP-deficient cells.
T16184 9366-9629 Sentence denotes CD40-mediated signaling was defective in HOIP-deficient cells (HOIP-/- + pMIP), but intact in parental cells transduced with an empty retroviral vector (A20.2J + pMIP) and in HOIP-deficient cells transduced with a retroviral vector encoding HOIP (HOIP-/- + HOIP).
T16185 9630-9710 Sentence denotes Cells were activated with CD154-expressing insect cells for the times indicated.
T16186 9711-9815 Sentence denotes As a negative control, cells were stimulated with insect cells lacking CD154 (5 minute time point only).
T16187 9816-9894 Sentence denotes Western blots of whole-cell lysates were probed with the indicated antibodies.
T16188 9895-10068 Sentence denotes Phospho-IκBα (pIκBα) and phospho-JNK (pJNK) blots were stripped and reprobed for total IκBα and total JNK, respectively (anti-JNK antibodies recognize p46 and p54 isoforms).
T16189 10069-10143 Sentence denotes IκBα blots were also reprobed for actin to demonstrate equal lane loading.
T16190 10144-10185 Sentence denotes Molecular weights are indicated at right.
T16191 10186-10308 Sentence denotes Similar results were obtained in a second experiment and in two additional experiments with a second HOIP-deficient clone.
T16813 12073-12156 Sentence denotes IKK recruitment to the CD40 signaling complex is defective in HOIP-deficient cells.
T16814 12157-12253 Sentence denotes (A) SMAC peptide treatment reduces recruitment of cIAP1 to CD40 and may modify HOIP recruitment.
T16815 12254-12380 Sentence denotes A20.2J cells were incubated for six hours with membrane-permeable SMAC-N7 peptide or 1.5% DMSO (solvent used for the peptide).
T16816 12381-12507 Sentence denotes Following the incubation, cell lysates were prepared, fractionated by SDS-PAGE, and evaluated by Western blot (lanes 1 and 2).
T16817 12508-12639 Sentence denotes Cells incubated with DMSO or SMAC-N7 were also stimulated with magnetic beads coated with anti-CD40 or an isotype control antibody.
T16818 12640-12716 Sentence denotes Immunoprecipitated (IP) material bound to the beads was loaded in lanes 3-5.
T16819 12717-12791 Sentence denotes Samples of the cell lysates after immunoprecipitation appear in lanes 6–8.
T16820 12792-12927 Sentence denotes Western blots were probed with antibodies specific for cIAP1, TRAF2, TRAF3, and HOIP (approximate molecular weights indicated on left).
T16821 12928-13176 Sentence denotes Similar results were obtained in two additional experiments. (B) CD40 was isolated by immunoprecipitation (as in (A)) from A20.2J cells and HOIP-deficient cells transduced with an empty retroviral vector (pMIP) or a retroviral vector encoding HOIP.
T16822 13177-13372 Sentence denotes Material immunoprecipitated with an isotype control antibody (isotype) or anti-CD40 antibody was examined by Western blotting for CD40, TRAF2, TRAF3, cIAP1, HOIP, IKKα/β, and IKKγ (right panels).
T16823 13373-13448 Sentence denotes HOIP expression was required for coprecipitation of IKK proteins with CD40.
T16824 13449-13515 Sentence denotes Cell lysates from unstimulated cells are shown in the left panels.
T16825 13516-13909 Sentence denotes Similar results were obtained in a second experiment and in two experiments with a second HOIP-deficient clone. (C) To further evaluate HOIP-dependent recruitment of IKKγ to CD40, A20.2J cells or HOIP-deficient (HOIP-/-) A20.2J cells were transduced with an empty retroviral vector or a retroviral construct encoding BP epitope-tagged IKKγ (noted in the figure as pMIP and IKKγ, respectively).
T16826 13910-14124 Sentence denotes Lysates (lanes 1–3) and immunoprecipitation (IP) samples (lanes 4–11) from the cell lines were fractionated by SDS-PAGE and evaluated by Western blotting with antibodies to the BP tag (IKKγ, upper panel) and TRAF2.
T16827 14125-14231 Sentence denotes The anti-CD40 IP sample in lane 11 was treated with λ phosphatase; the sample in lane 10 was mock-treated.
T16828 14232-14418 Sentence denotes Protein samples (minus those treated with phosphatase) were also fractionated on a separate gel (lower acrylamide concentration) for the evaluation of TRAF3 and HOIP (bottom two panels).
T16829 14419-14479 Sentence denotes Similar results were obtained in two additional experiments.
T34 0-7 Sentence denotes Results
T35 9-102 Sentence denotes Generation of HOIP-deficient B cells via targeted disruption of Rnf31, the gene encoding HOIP
T36 103-225 Sentence denotes In our previous study, we demonstrated that HOIP is recruited to the CD40 signaling complex in two mouse B cell lines [6].
T37 226-400 Sentence denotes Similar results were obtained with mouse splenocytes, indicating that the interaction of CD40 with HOIP is not limited to transformed B cell lines (unpublished observations).
T38 401-574 Sentence denotes We also found that the recruitment of HOIP to CD40 was TRAF2-dependent and that overexpression of a truncated HOIP mutant partially inhibited CD40-mediated NF-κB activation.
T39 575-663 Sentence denotes These results support the hypothesis that HOIP plays a role in CD40 signal transduction.
T40 664-848 Sentence denotes To further define the role and evaluate the importance of HOIP in CD40 signaling, we used somatic cell gene targeting to disrupt the gene encoding HOIP in the mouse B cell line A20.2J.
T41 849-1051 Sentence denotes This cell line has been particularly useful in the characterization of CD40 signaling mechanisms due to the relative ease with which its genome can be modified by homologous recombination [7], [8], [9].
T42 1052-1225 Sentence denotes We used a targeting vector capable of undergoing homologous recombination with Rnf31 (the gene encoding HOIP) to disrupt the coding sequence of the gene in exon 5 (Fig. 1A).
T43 1226-1409 Sentence denotes Following introduction of the vector, the neomycin-resistant clones that arose were screened by PCR amplification of genomic DNA to identify cells containing a disrupted Rnf31 allele.
T44 1410-1585 Sentence denotes To remove the selectable marker gene cassette from the disrupted Rnf31 allele, recombinant cell lines were transiently transfected with a plasmid that encodes Cre recombinase.
T45 1586-1728 Sentence denotes This step allowed us to perform a second round of targeting and drug selection, generating cells in which both copies of Rnf31 were disrupted.
T46 1729-1796 Sentence denotes Two independent clonal cell lines were chosen for further analysis.
T47 1797-1980 Sentence denotes HOIP protein expression was undetectable in both cell lines, as determined by Western blot analysis of cell lysates (Fig. 1B), demonstrating that the targeting process was successful.
T48 1981-2080 Sentence denotes In the text that follows, the two gene-targeted cell lines are referred to as HOIP-deficient cells.
T49 2081-2145 Sentence denotes 10.1371/journal.pone.0023061.g001 Figure 1 HOIP gene targeting.
T50 2146-2245 Sentence denotes (A) Regions of Rnf31 gene sequence (bottom) used in the HOIP gene targeting vector (top) are shown.
T51 2246-2398 Sentence denotes Arrows (F and R) indicate approximate positions of sequences homologous to oligonucleotides used for PCR-mediated detection of homologous recombination.
T52 2399-2600 Sentence denotes Homologous recombination of the vector with Rnf31 resulted in a small chromosomal deletion and the in-frame insertion of neomycin phosphotransferase (NeoR) into the amino-terminal HOIP coding sequence.
T53 2601-2756 Sentence denotes A diphtheria toxin (DT) cassette in the vector facilitates the negative selection of cells in which random chromosomal insertion of the vector takes place.
T54 2757-2832 Sentence denotes LoxP sequences allow the Cre-mediated deletion of the NeoR coding sequence.
T55 2833-3048 Sentence denotes The SV40pA sequence helps to ensure disruption of gene expression after deletion of the NeoR sequence. (B) Anti-HOIP and anti-FLAG Western blots of cell lysates from A20.2J cells and HOIP-deficient (HOIP-/-) clones.
T56 3049-3167 Sentence denotes A partial decrease in HOIP protein expression is evident in cells following disruption of one copy of Rnf31 (HOIP−/+).
T57 3168-3310 Sentence denotes HOIP expression in clones reconstituted with an empty retroviral vector (pMIP) or a retroviral vector encoding FLAG-tagged HOIP is also shown.
T58 3311-3358 Sentence denotes Approximate molecular weight of HOIP is 120 kD.
T59 3361-3652 Sentence denotes To enable us to confirm that any signaling or functional defects observed in HOIP-deficient cells were due to disruption of the Rnf31 gene, both HOIP-deficient cell lines were transduced with a retrovirus encoding FLAG-tagged wild-type HOIP, thus restoring HOIP protein expression (Fig. 1B).
T60 3653-3709 Sentence denotes These cells are referred to as HOIP-reconstituted cells.
T61 3710-3911 Sentence denotes In the experiments that follow, responses by these cells were compared to those of A20.2J cells and HOIP-deficient cells transduced with a retroviral vector (pMIP) lacking a cDNA insert (empty vector).
T62 3913-4014 Sentence denotes HOIP is required for CD40-mediated CD80 upregulation and activation of germline epsilon transcription
T63 4015-4180 Sentence denotes Engagement of CD40 on B cells upregulates expression of CD80 [10], a cell surface protein that promotes activation of T cells interacting with B cells and other APC.
T64 4181-4392 Sentence denotes To begin evaluating potential contributions of HOIP to CD40 signaling, we assayed the upregulation of CD80 induced by engagement of CD40 on parental A20.2J, HOIP-deficient, and HOIP-reconstituted cells (Fig. 2).
T65 4393-4530 Sentence denotes Compared to the parental cell line, the CD40-stimulated upregulation of CD80 was dramatically reduced in all HOIP-deficient cells tested.
T66 4531-4718 Sentence denotes In contrast, upregulation of CD80 was restored in HOIP-reconstituted cells, confirming that the defect in CD80 upregulation in the HOIP-deficient cells was due to the disruption of Rnf31.
T67 4719-4832 Sentence denotes 10.1371/journal.pone.0023061.g002 Figure 2 CD40-mediated CD80 upregulation is defective in HOIP-deficient cells.
T68 4833-5096 Sentence denotes Cells were incubated for 72 hrs with an isotype control antibody (left) or anti-CD40 antibody (right) and then stained for expression of CD80 (filled profiles indicate staining with anti-CD80 antibody; open profiles are staining with an isotype control antibody).
T69 5097-5280 Sentence denotes Transduction of both HOIP-deficient (HOIP-/-) clones with a retrovirus encoding FLAG-HOIP reversed defective CD80 upregulation, while transduction with an empty vector (pMIP) did not.
T70 5281-5334 Sentence denotes Similar results were obtained in a second experiment.
T71 5337-5471 Sentence denotes CD40 signals in B cells can also contribute to the activation of DNA recombination in the immunoglobulin heavy chain locus [11], [12].
T72 5472-5707 Sentence denotes This process, known as immunoglobulin class switching (or isotype switching), allows B cells, initially expressing IgM, to switch to IgA, IgG, or IgE depending on the class of antibody most appropriate for a particular immune response.
T73 5708-5784 Sentence denotes Switching to IgE can be induced by the combination of IL-4 and CD40 signals.
T74 5785-5955 Sentence denotes The gene rearrangement necessary for IgE production is preceded by the production of non-coding RNA transcripts from germline sequences in the IgE heavy chain locus [13].
T75 5956-6196 Sentence denotes To determine whether HOIP is required in the initiation of the class switching process, the relevant B cell lines were stimulated through CD40 in the presence of IL-4 and tested for production of germline epsilon (GLε) transcripts (Fig. 3).
T76 6197-6319 Sentence denotes Anti-CD40 antibody stimulation alone or together with IL-4 induced GLε transcription in parental A20.2J cells as expected.
T77 6320-6475 Sentence denotes In contrast, only low levels of GLε expression were detected in HOIP-deficient cells stimulated with either anti-CD40 antibody alone or anti-CD40 and IL-4.
T78 6476-6612 Sentence denotes Normal levels of GLε expression were induced in HOIP-reconstituted cells stimulated with anti-CD40 antibody alone or together with IL-4.
T79 6613-6720 Sentence denotes Collectively, these data demonstrate that HOIP has a critical role in cellular responses to CD40 signaling.
T80 6721-6836 Sentence denotes 10.1371/journal.pone.0023061.g003 Figure 3 GLε transcription is defective in CD40-stimulated HOIP-deficient cells.
T81 6837-7154 Sentence denotes A20.2J and HOIP-deficient cells transduced with an empty retroviral vector (A20.2J + pMIP and HOIP-/- + pMIP, respectively), or HOIP-deficient cells transduced with a HOIP-encoding retrovirus (HOIP-/- + HOIP) were cultured overnight with agonistic anti-CD40 or an isotype control antibody (iso), with or without IL-4.
T82 7155-7287 Sentence denotes RNA was isolated from the cells, reverse-transcribed, and then subjected to quantitative PCR to determine levels of GLε transcripts.
T83 7288-7362 Sentence denotes Results were normalized to the levels of Hprt1 transcripts in each sample.
T84 7363-7461 Sentence denotes Symbols indicate the values from duplicate cultures (a line indicates the mean of the two values).
T85 7462-7582 Sentence denotes Similar results were obtained in a second experiment and in an additional experiment with a second HOIP-deficient clone.
T86 7584-7638 Sentence denotes HOIP mediates CD40-stimulated NF-κB and JNK activation
T87 7639-7753 Sentence denotes The results described above show that HOIP plays an important role in CD40-mediated effector functions of B cells.
T88 7754-7818 Sentence denotes It follows that HOIP is likely a key mediator of CD40 signaling.
T89 7819-8075 Sentence denotes To test this hypothesis, we stimulated A20.2J and HOIP-deficient cells with CD154 (CD40 ligand) expressed by HI5 insect cells [7], [8] and measured activation of the NF-κB and JNK pathways, two of the major transcriptional regulators activated by CD40 [4].
T90 8076-8267 Sentence denotes Cell-associated CD154 was used as the stimulus in these experiments as it typically provides more robust, and therefore more readily detected, activation signals than does anti-CD40 antibody.
T91 8268-8396 Sentence denotes Activation of the canonical NF-κB pathway is initiated with the phosphorylation of IκB proteins by the IκB kinase complex (IKK).
T92 8397-8493 Sentence denotes In resting cells, IκB proteins are responsible for sequestering NF-κB subunits in the cytoplasm.
T93 8494-8651 Sentence denotes Phosphorylation by the IKK complex targets IκB proteins for ubiquitination and degradation, allowing NF-κB to enter the nucleus and activate gene expression.
T94 8652-8816 Sentence denotes CD40-mediated phosphorylation and degradation of IκBα in HOIP-deficient cells was dramatically impaired relative to that observed in parental A20.2J cells (Fig. 4).
T95 8817-8927 Sentence denotes We also assayed activation of the stress-activated protein kinase JNK in response to CD40 engagement (Fig. 4).
T96 8928-9053 Sentence denotes CD40-mediated JNK activation in HOIP-deficient cells was impaired as measured by phosphorylation of Thr183 and Tyr185 in JNK.
T97 9054-9242 Sentence denotes CD40-induced activation of NF-κB and JNK in HOIP-reconstituted cells was normal, demonstrating that the defects observed in gene-deficient cells were due to the absence of HOIP expression.
T98 9243-9365 Sentence denotes 10.1371/journal.pone.0023061.g004 Figure 4 CD40-induced activation of NF-κB and JNK is defective in HOIP-deficient cells.
T99 9366-9629 Sentence denotes CD40-mediated signaling was defective in HOIP-deficient cells (HOIP-/- + pMIP), but intact in parental cells transduced with an empty retroviral vector (A20.2J + pMIP) and in HOIP-deficient cells transduced with a retroviral vector encoding HOIP (HOIP-/- + HOIP).
T100 9630-9710 Sentence denotes Cells were activated with CD154-expressing insect cells for the times indicated.
T101 9711-9815 Sentence denotes As a negative control, cells were stimulated with insect cells lacking CD154 (5 minute time point only).
T102 9816-9894 Sentence denotes Western blots of whole-cell lysates were probed with the indicated antibodies.
T103 9895-10068 Sentence denotes Phospho-IκBα (pIκBα) and phospho-JNK (pJNK) blots were stripped and reprobed for total IκBα and total JNK, respectively (anti-JNK antibodies recognize p46 and p54 isoforms).
T104 10069-10143 Sentence denotes IκBα blots were also reprobed for actin to demonstrate equal lane loading.
T105 10144-10185 Sentence denotes Molecular weights are indicated at right.
T106 10186-10308 Sentence denotes Similar results were obtained in a second experiment and in two additional experiments with a second HOIP-deficient clone.
T107 10310-10372 Sentence denotes HOIP is necessary for association of the IKK complex with CD40
T108 10373-10585 Sentence denotes The marked defects in CD40-mediated cell activation and signaling displayed by HOIP-deficient cells suggested that HOIP mediates recruitment of critical components of the CD40 signaling apparatus to the receptor.
T109 10586-10751 Sentence denotes Previously, we demonstrated that HOIP is recruited to the CD40 signaling complex in a TRAF2-dependent manner, suggesting that HOIP functions downstream of TRAF2 [6].
T110 10752-10891 Sentence denotes Studies by others suggest that the TRAF2-associated proteins cIAP1 and cIAP2 play a role in the recruitment of HOIP to TNFR1 and CD40 [14].
T111 10892-11121 Sentence denotes Therefore, we determined whether the association of HOIP with CD40 in A20.2J cells was altered by treatment of cells with an inhibitor of cIAP activity, a membrane-permeable peptide derived from the apoptosis regulator SMAC [15].
T112 11122-11331 Sentence denotes We found that pretreatment of cells with the SMAC peptide dramatically reduced the amount of cIAP1 associated with the CD40 signaling complex in cells stimulated with anti-CD40 antibody-coated beads (Fig. 5A).
T113 11332-11583 Sentence denotes SMAC peptide treatment also resulted in a slight but reproducible decrease in the amount of the major HOIP form recovered by CD40 immunoprecipitation, along with an apparent increase in higher molecular weight species recognized by anti-HOIP antibody.
T114 11584-11706 Sentence denotes In contrast, treatment with the SMAC peptide did not alter the amount or molecular weight of HOIP present in cell lysates.
T115 11707-11871 Sentence denotes These data suggest that SMAC peptide treatment specifically alters the characteristics of CD40-associated HOIP rather than the entire cellular pool of this protein.
T116 11872-12028 Sentence denotes Together, these results support the idea that the cIAP proteins influence the recruitment and post-translational modification state of CD40-associated HOIP.
T117 12029-12156 Sentence denotes 10.1371/journal.pone.0023061.g005 Figure 5 IKK recruitment to the CD40 signaling complex is defective in HOIP-deficient cells.
T118 12157-12253 Sentence denotes (A) SMAC peptide treatment reduces recruitment of cIAP1 to CD40 and may modify HOIP recruitment.
T119 12254-12380 Sentence denotes A20.2J cells were incubated for six hours with membrane-permeable SMAC-N7 peptide or 1.5% DMSO (solvent used for the peptide).
T120 12381-12507 Sentence denotes Following the incubation, cell lysates were prepared, fractionated by SDS-PAGE, and evaluated by Western blot (lanes 1 and 2).
T121 12508-12639 Sentence denotes Cells incubated with DMSO or SMAC-N7 were also stimulated with magnetic beads coated with anti-CD40 or an isotype control antibody.
T122 12640-12716 Sentence denotes Immunoprecipitated (IP) material bound to the beads was loaded in lanes 3-5.
T123 12717-12791 Sentence denotes Samples of the cell lysates after immunoprecipitation appear in lanes 6–8.
T124 12792-12927 Sentence denotes Western blots were probed with antibodies specific for cIAP1, TRAF2, TRAF3, and HOIP (approximate molecular weights indicated on left).
T125 12928-13176 Sentence denotes Similar results were obtained in two additional experiments. (B) CD40 was isolated by immunoprecipitation (as in (A)) from A20.2J cells and HOIP-deficient cells transduced with an empty retroviral vector (pMIP) or a retroviral vector encoding HOIP.
T126 13177-13372 Sentence denotes Material immunoprecipitated with an isotype control antibody (isotype) or anti-CD40 antibody was examined by Western blotting for CD40, TRAF2, TRAF3, cIAP1, HOIP, IKKα/β, and IKKγ (right panels).
T127 13373-13448 Sentence denotes HOIP expression was required for coprecipitation of IKK proteins with CD40.
T128 13449-13515 Sentence denotes Cell lysates from unstimulated cells are shown in the left panels.
T129 13516-13909 Sentence denotes Similar results were obtained in a second experiment and in two experiments with a second HOIP-deficient clone. (C) To further evaluate HOIP-dependent recruitment of IKKγ to CD40, A20.2J cells or HOIP-deficient (HOIP-/-) A20.2J cells were transduced with an empty retroviral vector or a retroviral construct encoding BP epitope-tagged IKKγ (noted in the figure as pMIP and IKKγ, respectively).
T130 13910-14124 Sentence denotes Lysates (lanes 1–3) and immunoprecipitation (IP) samples (lanes 4–11) from the cell lines were fractionated by SDS-PAGE and evaluated by Western blotting with antibodies to the BP tag (IKKγ, upper panel) and TRAF2.
T131 14125-14231 Sentence denotes The anti-CD40 IP sample in lane 11 was treated with λ phosphatase; the sample in lane 10 was mock-treated.
T132 14232-14418 Sentence denotes Protein samples (minus those treated with phosphatase) were also fractionated on a separate gel (lower acrylamide concentration) for the evaluation of TRAF3 and HOIP (bottom two panels).
T133 14419-14479 Sentence denotes Similar results were obtained in two additional experiments.
T134 14482-14703 Sentence denotes To test the possibility that HOIP is responsible for the recruitment of other critical signaling proteins to CD40, we immunoprecipitated CD40 signaling complexes from HOIP-deficient and HOIP-reconstituted cells (Fig. 5B).
T135 14704-14852 Sentence denotes As described previously, CD40 signaling complexes immunoprecipitated from A20.2J cells contained HOIP as well as TRAF2, TRAF3, IKKα/β, and IKKγ [6].
T136 14853-15080 Sentence denotes The amounts of TRAF2, TRAF3, and cIAP1 in CD40 immunoprecipitates from HOIP-deficient cells were similar to those from parental A20.2J cells, indicating that HOIP is not required for the association of these proteins with CD40.
T137 15081-15205 Sentence denotes In contrast, IKKα/β and IKKγ were not detectable in CD40 immunoprecipitates recovered from HOIP-deficient cells (Fig. 5B,C).
T138 15206-15484 Sentence denotes The amounts of IKKα/β and IKKγ in CD40 immunoprecipitates from HOIP-reconstituted cells were similar to those detected in samples prepared from parental cells, demonstrating that the defects in IKK recruitment we observed were due specifically to the absence of HOIP expression.
T139 15485-15605 Sentence denotes These data indicate that HOIP is required for recruitment of the NF-κB-activating complex to the CD40 signaling complex.
T140 15606-15765 Sentence denotes As shown here and in our previous study [6], IKKγ present in CD40 immunoprecipitates appears to have a higher molecular weight than that found in cell lysates.
T141 15766-15969 Sentence denotes To confirm that this higher molecular weight species was indeed IKKγ, we generated A20.2J cell lines containing a stably integrated retroviral vector that encoded an epitope-tagged version of mouse IKKγ.
T142 15970-16101 Sentence denotes Cell lysates and CD40 immunoprecipitates prepared from these cells were analyzed by Western blotting for the epitope tag (Fig. 5C).
T143 16102-16269 Sentence denotes This analysis produced a pattern of bands that was essentially the same as that obtained using an antibody specific for native IKKγ (compare panels B and C in Fig. 5).
T144 16270-16386 Sentence denotes Moreover, analysis of cells expressing epitope tagged-IKKγ confirmed that recruitment of IKKγ to CD40 requires HOIP.
T145 16387-16641 Sentence denotes To determine what was responsible for the increased molecular weight of IKKγ in CD40 immunoprecipitates, the protein samples were incubated with lambda phosphatase, which removes phosphates attached to tyrosine, threonine, or serine residues in proteins.
T146 16642-16783 Sentence denotes This treatment reduced much of CD40-associated IKKγ to an apparent molecular weight similar to that of the protein in cell lysates (Fig. 5C).
T147 16784-17004 Sentence denotes However, at least one band of significantly higher molecular weight remained after phosphatase treatment, suggesting that CD40-associated IKKγ is subject to at least one other modification in addition to phosphorylation.
T148 17005-17181 Sentence denotes Overall, these data demonstrate that HOIP is required for the association of IKKγ with CD40, and suggest that this event is coupled to post-translational modifications of IKKγ.

ICD10

Id Subject Object Predicate Lexical cue
T15255 2603-2613 http://purl.bioontology.org/ontology/ICD10/A36.9 denotes diphtheria
T15256 2603-2613 http://purl.bioontology.org/ontology/ICD10/A36 denotes diphtheria

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T3782 23-27 Q96EP0 denotes HOIP
T3783 73-78 Q96EP0 denotes Rnf31
T3784 98-102 Q96EP0 denotes HOIP
T3785 147-151 Q96EP0 denotes HOIP
T3786 172-176 P25942 denotes CD40
T3787 315-319 P25942 denotes CD40
T3788 325-329 Q96EP0 denotes HOIP
T3789 439-443 Q96EP0 denotes HOIP
T3790 447-451 P25942 denotes CD40
T3791 456-461 Q12933 denotes TRAF2
T3792 511-515 Q96EP0 denotes HOIP
T3793 543-547 P25942 denotes CD40
T3794 617-621 Q96EP0 denotes HOIP
T3795 638-642 P25942 denotes CD40
T3796 722-726 Q96EP0 denotes HOIP
T3797 730-734 P25942 denotes CD40
T3798 811-815 Q96EP0 denotes HOIP
T3799 841-844 P21580 denotes A20
T3800 920-924 P25942 denotes CD40
T3801 957-965 Q04864 denotes relative
T3802 1131-1136 Q96EP0 denotes Rnf31
T3803 1156-1160 Q96EP0 denotes HOIP
T3804 1396-1401 Q96EP0 denotes Rnf31
T3805 1475-1480 Q96EP0 denotes Rnf31
T3806 1569-1572 P06956 denotes Cre
T3807 1707-1712 Q96EP0 denotes Rnf31
T3808 1797-1801 Q96EP0 denotes HOIP
T3809 2059-2063 Q96EP0 denotes HOIP
T3810 3438-3442 Q96EP0 denotes HOIP
T3811 3489-3494 Q96EP0 denotes Rnf31
T3812 3506-3510 Q96EP0 denotes HOIP
T3813 3597-3601 Q96EP0 denotes HOIP
T3814 3618-3622 Q96EP0 denotes HOIP
T3815 3684-3688 Q96EP0 denotes HOIP
T3816 3793-3796 P21580 denotes A20
T3817 3810-3814 Q96EP0 denotes HOIP
T5002 3913-3917 Q96EP0 denotes HOIP
T5003 3934-3938 P25942 denotes CD40
T5004 3948-3952 P33681 denotes CD80
T5005 3984-4000 Q28322 denotes germline epsilon
T5006 4029-4033 P25942 denotes CD40
T5007 4071-4075 P33681 denotes CD80
T5008 4228-4232 Q96EP0 denotes HOIP
T5009 4236-4240 P25942 denotes CD40
T5010 4283-4287 P33681 denotes CD80
T5011 4313-4317 P25942 denotes CD40
T5012 4330-4333 P21580 denotes A20
T5013 4338-4342 Q96EP0 denotes HOIP
T5014 4358-4362 Q96EP0 denotes HOIP
T5015 4433-4437 P25942 denotes CD40
T5016 4465-4469 P33681 denotes CD80
T5017 4502-4506 Q96EP0 denotes HOIP
T5018 4560-4564 P33681 denotes CD80
T5019 4581-4585 Q96EP0 denotes HOIP
T5020 4637-4641 P33681 denotes CD80
T5021 4662-4666 Q96EP0 denotes HOIP
T5022 4712-4717 Q96EP0 denotes Rnf31
T5023 5337-5341 P25942 denotes CD40
T5024 5427-5453 P01860 denotes immunoglobulin heavy chain
T5025 5427-5453 P01877 denotes immunoglobulin heavy chain
T5026 5427-5453 P01871 denotes immunoglobulin heavy chain
T5027 5427-5453 P01876 denotes immunoglobulin heavy chain
T5028 5427-5453 P01859 denotes immunoglobulin heavy chain
T5029 5427-5453 P01861 denotes immunoglobulin heavy chain
T5030 5427-5453 P01854 denotes immunoglobulin heavy chain
T5031 5427-5453 P01857 denotes immunoglobulin heavy chain
T5032 5427-5453 P01880 denotes immunoglobulin heavy chain
T5033 5762-5766 P05112 denotes IL-4
T5034 5771-5775 P25942 denotes CD40
T5035 5977-5981 Q96EP0 denotes HOIP
T5036 6094-6098 P25942 denotes CD40
T5037 6118-6122 P05112 denotes IL-4
T5038 6152-6168 Q28322 denotes germline epsilon
T5039 6170-6173 Q28322 denotes GLε
T5040 6202-6206 P25942 denotes CD40
T5041 6251-6255 P05112 denotes IL-4
T5042 6264-6267 Q28322 denotes GLε
T5043 6294-6297 P21580 denotes A20
T5044 6352-6355 Q28322 denotes GLε
T5045 6384-6388 Q96EP0 denotes HOIP
T5046 6433-6437 P25942 denotes CD40
T5047 6461-6465 P25942 denotes CD40
T5048 6470-6474 P05112 denotes IL-4
T5049 6493-6496 Q28322 denotes GLε
T5050 6524-6528 Q96EP0 denotes HOIP
T5051 6570-6574 P25942 denotes CD40
T5052 6607-6611 P05112 denotes IL-4
T5053 6655-6659 Q96EP0 denotes HOIP
T5054 6705-6709 P25942 denotes CD40
T6055 7584-7588 Q96EP0 denotes HOIP
T6056 7598-7602 P25942 denotes CD40
T6057 7677-7681 Q96EP0 denotes HOIP
T6058 7709-7713 P25942 denotes CD40
T6059 7770-7774 Q96EP0 denotes HOIP
T6060 7803-7807 P25942 denotes CD40
T6061 7858-7861 P21580 denotes A20
T6062 7869-7873 Q96EP0 denotes HOIP
T6063 7895-7900 P29965 denotes CD154
T6064 7902-7906 P25942 denotes CD40
T6065 7902-7913 P29965 denotes CD40 ligand
T6066 8066-8070 P25942 denotes CD40
T6067 8092-8097 P29965 denotes CD154
T6068 8253-8257 P25942 denotes CD40
T6069 8371-8381 O15111 denotes IκB kinase
T6070 8371-8381 Q60680-2 denotes IκB kinase
T6071 8652-8656 P25942 denotes CD40
T6072 8701-8705 P25963 denotes IκBα
T6073 8709-8713 Q96EP0 denotes HOIP
T6074 8756-8764 Q04864 denotes relative
T6075 8794-8797 P21580 denotes A20
T6076 8851-8882 O15264 denotes stress-activated protein kinase
T6077 8851-8882 P53778 denotes stress-activated protein kinase
T6078 8851-8886 P53779 denotes stress-activated protein kinase JNK
T6079 8902-8906 P25942 denotes CD40
T6080 8928-8932 P25942 denotes CD40
T6081 8960-8964 Q96EP0 denotes HOIP
T6082 9054-9058 P25942 denotes CD40
T6083 9098-9102 Q96EP0 denotes HOIP
T6084 9226-9230 Q96EP0 denotes HOIP
T8191 10368-10372 P25942 denotes CD40
T8192 10395-10399 P25942 denotes CD40
T8193 10452-10456 Q96EP0 denotes HOIP
T8194 10488-10492 Q96EP0 denotes HOIP
T8195 10544-10548 P25942 denotes CD40
T8196 10619-10623 Q96EP0 denotes HOIP
T8197 10644-10648 P25942 denotes CD40
T8198 10672-10677 Q12933 denotes TRAF2
T8199 10712-10716 Q96EP0 denotes HOIP
T8200 10741-10746 Q12933 denotes TRAF2
T8201 10787-10792 Q12933 denotes TRAF2
T8202 10813-10818 Q13490 denotes cIAP1
T8203 10863-10867 Q96EP0 denotes HOIP
T8204 10881-10885 P25942 denotes CD40
T8205 10944-10948 Q96EP0 denotes HOIP
T8206 10954-10958 P25942 denotes CD40
T8207 10962-10965 P21580 denotes A20
T8208 11215-11220 Q13490 denotes cIAP1
T8209 11241-11245 P25942 denotes CD40
T8210 11294-11298 P25942 denotes CD40
T8211 11434-11438 Q96EP0 denotes HOIP
T8212 11457-11461 P25942 denotes CD40
T8213 11569-11573 Q96EP0 denotes HOIP
T8214 11677-11681 Q96EP0 denotes HOIP
T8215 11797-11801 P25942 denotes CD40
T8216 11813-11817 Q96EP0 denotes HOIP
T8217 12007-12011 P25942 denotes CD40
T8218 12023-12027 Q96EP0 denotes HOIP
T8219 14511-14515 Q96EP0 denotes HOIP
T8220 14591-14595 P25942 denotes CD40
T8221 14619-14623 P25942 denotes CD40
T8222 14649-14653 Q96EP0 denotes HOIP
T8223 14668-14672 Q96EP0 denotes HOIP
T8224 14729-14733 P25942 denotes CD40
T8225 14778-14781 P21580 denotes A20
T8226 14801-14805 Q96EP0 denotes HOIP
T8227 14817-14822 Q12933 denotes TRAF2
T8228 14824-14829 Q13114 denotes TRAF3
T8229 14868-14873 Q12933 denotes TRAF2
T8230 14875-14880 Q13114 denotes TRAF3
T8231 14886-14891 Q13490 denotes cIAP1
T8232 14895-14899 P25942 denotes CD40
T8233 14924-14928 Q96EP0 denotes HOIP
T8234 14981-14984 P21580 denotes A20
T8235 15011-15015 Q96EP0 denotes HOIP
T8236 15075-15079 P25942 denotes CD40
T8237 15133-15137 P25942 denotes CD40
T8238 15172-15176 Q96EP0 denotes HOIP
T8239 15240-15244 P25942 denotes CD40
T8240 15269-15273 Q96EP0 denotes HOIP
T8241 15468-15472 Q96EP0 denotes HOIP
T8242 15510-15514 Q96EP0 denotes HOIP
T8243 15582-15586 P25942 denotes CD40
T8244 15667-15671 P25942 denotes CD40
T8245 15849-15852 P21580 denotes A20
T8246 15987-15991 P25942 denotes CD40
T8247 16367-16371 P25942 denotes CD40
T8248 16381-16385 Q96EP0 denotes HOIP
T8249 16467-16471 P25942 denotes CD40
T8250 16673-16677 P25942 denotes CD40
T8251 16906-16910 P25942 denotes CD40
T8252 17042-17046 Q96EP0 denotes HOIP
T8253 17092-17096 P25942 denotes CD40
T15228 2125-2129 Q96EP0 denotes HOIP
T15229 2161-2166 Q96EP0 denotes Rnf31
T15230 2202-2206 Q96EP0 denotes HOIP
T15231 2443-2448 Q96EP0 denotes Rnf31
T15232 2579-2583 Q96EP0 denotes HOIP
T15233 2782-2785 P06956 denotes Cre
T15234 2945-2949 Q96EP0 denotes HOIP
T15235 2999-3002 P21580 denotes A20
T15236 3016-3020 Q96EP0 denotes HOIP
T15237 3032-3036 Q96EP0 denotes HOIP
T15238 3071-3075 Q96EP0 denotes HOIP
T15239 3151-3156 Q96EP0 denotes Rnf31
T15240 3158-3162 Q96EP0 denotes HOIP
T15241 3168-3172 Q96EP0 denotes HOIP
T15242 3291-3295 Q96EP0 denotes HOIP
T15243 3343-3347 Q96EP0 denotes HOIP
T15581 4763-4767 P25942 denotes CD40
T15582 4777-4781 P33681 denotes CD80
T15583 4811-4815 Q96EP0 denotes HOIP
T15584 4913-4917 P25942 denotes CD40
T15585 4970-4974 P33681 denotes CD80
T15586 5020-5024 P33681 denotes CD80
T15587 5118-5122 Q96EP0 denotes HOIP
T15588 5134-5138 Q96EP0 denotes HOIP
T15589 5182-5186 Q96EP0 denotes HOIP
T15590 5206-5210 P33681 denotes CD80
T16020 6765-6768 Q28322 denotes GLε
T16021 6799-6803 P25942 denotes CD40
T16022 6815-6819 Q96EP0 denotes HOIP
T16023 6837-6840 P21580 denotes A20
T16024 6848-6852 Q96EP0 denotes HOIP
T16025 6913-6916 P21580 denotes A20
T16026 6931-6935 Q96EP0 denotes HOIP
T16027 6965-6969 Q96EP0 denotes HOIP
T16028 7004-7008 Q96EP0 denotes HOIP
T16029 7030-7034 Q96EP0 denotes HOIP
T16030 7040-7044 Q96EP0 denotes HOIP
T16031 7090-7094 P25942 denotes CD40
T16032 7149-7153 P05112 denotes IL-4
T16033 7271-7274 Q28322 denotes GLε
T16034 7561-7565 Q96EP0 denotes HOIP
T16539 9287-9291 P25942 denotes CD40
T16540 9344-9348 Q96EP0 denotes HOIP
T16541 9366-9370 P25942 denotes CD40
T16542 9407-9411 Q96EP0 denotes HOIP
T16543 9429-9433 Q96EP0 denotes HOIP
T16544 9519-9522 P21580 denotes A20
T16545 9541-9545 Q96EP0 denotes HOIP
T16546 9607-9611 Q96EP0 denotes HOIP
T16547 9613-9617 Q96EP0 denotes HOIP
T16548 9623-9627 Q96EP0 denotes HOIP
T16549 9656-9661 P29965 denotes CD154
T16550 9782-9787 P29965 denotes CD154
T16551 9903-9907 P25963 denotes IκBα
T16552 9982-9986 P25963 denotes IκBα
T16553 10054-10057 Q13451 denotes p54
T16554 10069-10073 P25963 denotes IκBα
T16555 10287-10291 Q96EP0 denotes HOIP
T17697 12096-12100 P25942 denotes CD40
T17698 12135-12139 Q96EP0 denotes HOIP
T17699 12207-12212 Q13490 denotes cIAP1
T17700 12216-12220 P25942 denotes CD40
T17701 12236-12240 Q96EP0 denotes HOIP
T17702 12254-12257 P21580 denotes A20
T17703 12603-12607 P25942 denotes CD40
T17704 12847-12852 Q13490 denotes cIAP1
T17705 12854-12859 Q12933 denotes TRAF2
T17706 12861-12866 Q13114 denotes TRAF3
T17707 12872-12876 Q96EP0 denotes HOIP
T17708 12993-12997 P25942 denotes CD40
T17709 13051-13054 P21580 denotes A20
T17710 13068-13072 Q96EP0 denotes HOIP
T17711 13171-13175 Q96EP0 denotes HOIP
T17712 13256-13260 P25942 denotes CD40
T17713 13307-13311 P25942 denotes CD40
T17714 13313-13318 Q12933 denotes TRAF2
T17715 13320-13325 Q13114 denotes TRAF3
T17716 13327-13332 Q13490 denotes cIAP1
T17717 13334-13338 Q96EP0 denotes HOIP
T17718 13373-13377 Q96EP0 denotes HOIP
T17719 13443-13447 P25942 denotes CD40
T17720 13606-13610 Q96EP0 denotes HOIP
T17721 13652-13656 Q96EP0 denotes HOIP
T17722 13690-13694 P25942 denotes CD40
T17723 13696-13699 P21580 denotes A20
T17724 13712-13716 Q96EP0 denotes HOIP
T17725 13728-13732 Q96EP0 denotes HOIP
T17726 13737-13740 P21580 denotes A20
T17727 14118-14123 Q12933 denotes TRAF2
T17728 14134-14138 P25942 denotes CD40
T17729 14383-14388 Q13114 denotes TRAF3
T17730 14393-14397 Q96EP0 denotes HOIP

test2

Id Subject Object Predicate Lexical cue Negation Speculation
T2762 23-27 Protein denotes HOIP
T2763 28-37 Negative_regulation denotes deficient
T2764 59-69 Negative_regulation denotes disruption
T2765 73-78 Protein denotes Rnf31
T2766 98-102 Protein denotes HOIP
T2767 147-151 Protein denotes HOIP
T2768 155-164 Binding denotes recruited
T2769 300-311 Binding denotes interaction
T2770 315-319 Protein denotes CD40
T2771 325-329 Protein denotes HOIP
T2772 424-435 Binding denotes recruitment true true
T2773 439-443 Protein denotes HOIP
T2774 447-451 Protein denotes CD40
T2775 456-461 Protein denotes TRAF2
T2776 462-471 Regulation denotes dependent
T2777 511-515 Protein denotes HOIP
T2778 543-547 Protein denotes CD40
T2779 617-621 Protein denotes HOIP
T2780 638-642 Protein denotes CD40
T2781 722-726 Protein denotes HOIP
T2782 730-734 Protein denotes CD40
T2783 785-792 Negative_regulation denotes disrupt
T2784 811-815 Protein denotes HOIP
T2785 920-924 Protein denotes CD40
T2786 1131-1136 Protein denotes Rnf31
T2787 1156-1160 Protein denotes HOIP
T2788 1386-1395 Negative_regulation denotes disrupted
T2789 1396-1401 Protein denotes Rnf31
T2790 1465-1474 Negative_regulation denotes disrupted
T2791 1475-1480 Protein denotes Rnf31
T2792 1569-1584 Protein denotes Cre recombinase
T2793 1707-1712 Protein denotes Rnf31
T2794 1718-1727 Negative_regulation denotes disrupted
T2795 1797-1801 Protein denotes HOIP
T2796 1810-1820 Gene_expression denotes expression
T2797 2059-2063 Protein denotes HOIP
T2798 2064-2073 Negative_regulation denotes deficient
T2799 3438-3442 Protein denotes HOIP
T2800 3443-3452 Negative_regulation denotes deficient
T2801 3471-3481 Negative_regulation denotes disruption
T2802 3489-3494 Protein denotes Rnf31
T2803 3506-3510 Protein denotes HOIP
T2804 3511-3520 Negative_regulation denotes deficient
T2805 3597-3601 Protein denotes HOIP
T2806 3608-3617 Negative_regulation denotes restoring
T2807 3618-3622 Protein denotes HOIP
T2808 3631-3641 Gene_expression denotes expression
T2809 3684-3688 Protein denotes HOIP
T2810 3810-3814 Protein denotes HOIP
T2811 3815-3824 Negative_regulation denotes deficient
T4105 3913-3917 Protein denotes HOIP
T4106 3921-3929 Positive_regulation denotes required
T4107 3934-3938 Protein denotes CD40
T4108 3939-3947 Positive_regulation denotes mediated
T4109 3948-3952 Protein denotes CD80
T4110 3953-3965 Positive_regulation denotes upregulation
T4111 3984-4000 Protein denotes germline epsilon
T4112 4015-4025 Binding denotes Engagement
T4113 4029-4033 Protein denotes CD40
T4114 4045-4056 Positive_regulation denotes upregulates
T4115 4057-4067 Gene_expression denotes expression
T4116 4071-4075 Protein denotes CD80 true
T4117 4228-4232 Protein denotes HOIP
T4118 4236-4240 Protein denotes CD40
T4119 4267-4279 Positive_regulation denotes upregulation
T4120 4283-4287 Protein denotes CD80
T4121 4288-4295 Positive_regulation denotes induced
T4122 4299-4309 Binding denotes engagement
T4123 4313-4317 Protein denotes CD40
T4124 4338-4342 Protein denotes HOIP
T4125 4343-4352 Negative_regulation denotes deficient
T4126 4358-4362 Protein denotes HOIP
T4127 4363-4376 Positive_regulation denotes reconstituted
T4128 4433-4437 Protein denotes CD40
T4129 4438-4448 Positive_regulation denotes stimulated
T4130 4449-4461 Positive_regulation denotes upregulation true
T4131 4465-4469 Protein denotes CD80
T4132 4487-4494 Negative_regulation denotes reduced
T4133 4502-4506 Protein denotes HOIP
T4134 4507-4516 Negative_regulation denotes deficient
T4135 4544-4556 Positive_regulation denotes upregulation
T4136 4560-4564 Protein denotes CD80
T4137 4581-4585 Protein denotes HOIP
T4138 4586-4599 Positive_regulation denotes reconstituted
T4139 4627-4633 Negative_regulation denotes defect
T4140 4637-4641 Protein denotes CD80
T4141 4642-4654 Positive_regulation denotes upregulation
T4142 4662-4666 Protein denotes HOIP
T4143 4667-4676 Negative_regulation denotes deficient
T4144 4687-4690 Positive_regulation denotes due
T4145 4698-4708 Negative_regulation denotes disruption
T4146 4712-4717 Protein denotes Rnf31
T4147 5337-5341 Protein denotes CD40
T4148 5732-5739 Positive_regulation denotes induced
T4149 5762-5766 Protein denotes IL-4
T4150 5771-5775 Protein denotes CD40
T4151 5977-5981 Protein denotes HOIP
T4152 6094-6098 Protein denotes CD40
T4153 6118-6122 Protein denotes IL-4
T4154 6138-6148 Gene_expression denotes production
T4155 6152-6168 Protein denotes germline epsilon
T4156 6170-6173 Protein denotes GLε
T4157 6251-6255 Protein denotes IL-4
T4158 6256-6263 Positive_regulation denotes induced
T4159 6264-6267 Protein denotes GLε
T4160 6268-6281 Transcription denotes transcription
T4161 6352-6355 Protein denotes GLε
T4162 6356-6366 Gene_expression denotes expression
T4163 6384-6388 Protein denotes HOIP
T4164 6389-6398 Negative_regulation denotes deficient
T4165 6470-6474 Protein denotes IL-4
T4166 6493-6496 Protein denotes GLε
T4167 6497-6507 Gene_expression denotes expression
T4168 6513-6520 Positive_regulation denotes induced
T4169 6524-6528 Protein denotes HOIP
T4170 6529-6542 Positive_regulation denotes reconstituted
T4171 6607-6611 Protein denotes IL-4
T4172 6655-6659 Protein denotes HOIP
T4173 6705-6709 Protein denotes CD40
T5423 7584-7588 Protein denotes HOIP
T5424 7598-7602 Protein denotes CD40
T5425 7677-7681 Protein denotes HOIP
T5426 7709-7713 Protein denotes CD40
T5427 7770-7774 Protein denotes HOIP
T5428 7803-7807 Protein denotes CD40
T5429 7874-7883 Negative_regulation denotes deficient
T5430 7895-7900 Protein denotes CD154
T5431 7902-7913 Protein denotes CD40 ligand
T5432 7915-7924 Gene_expression denotes expressed
T5433 8066-8070 Protein denotes CD40
T5434 8092-8097 Protein denotes CD154
T5435 8461-8475 Protein denotes NF-κB subunits
T5436 8494-8509 Phosphorylation denotes Phosphorylation
T5437 8573-8584 Protein_catabolism denotes degradation true
T5438 8586-8594 Positive_regulation denotes allowing
T5439 8614-8621 Entity denotes nucleus
T5440 8652-8656 Protein denotes CD40
T5441 8657-8665 Positive_regulation denotes mediated
T5442 8666-8681 Phosphorylation denotes phosphorylation
T5443 8686-8697 Protein_catabolism denotes degradation
T5444 8701-8705 Protein denotes IκBα
T5445 8709-8713 Protein denotes HOIP
T5446 8714-8723 Negative_regulation denotes deficient
T5447 8747-8755 Negative_regulation denotes impaired
T5448 8902-8906 Protein denotes CD40
T5449 8907-8917 Binding denotes engagement
T5450 8928-8932 Protein denotes CD40
T5451 8960-8964 Protein denotes HOIP
T5452 8965-8974 Negative_regulation denotes deficient
T5453 8985-8993 Negative_regulation denotes impaired
T5454 9054-9058 Protein denotes CD40
T5455 9098-9102 Protein denotes HOIP
T5456 9103-9116 Positive_regulation denotes reconstituted
T5457 9183-9192 Negative_regulation denotes deficient
T5458 9215-9222 Negative_regulation denotes absence
T5459 9226-9230 Protein denotes HOIP
T5460 9231-9241 Gene_expression denotes expression
T6454 10368-10372 Protein denotes CD40
T6455 10395-10399 Protein denotes CD40
T6456 10452-10456 Protein denotes HOIP true
T6457 10457-10466 Negative_regulation denotes deficient
T6458 10488-10492 Protein denotes HOIP
T6459 10619-10623 Protein denotes HOIP
T6460 10627-10636 Binding denotes recruited
T6461 10672-10677 Protein denotes TRAF2
T6462 10678-10687 Regulation denotes dependent
T6463 10712-10716 Protein denotes HOIP
T6464 10727-10737 Regulation denotes downstream
T6465 10741-10746 Protein denotes TRAF2
T6466 10787-10792 Protein denotes TRAF2 true
T6467 10793-10803 Binding denotes associated
T6468 10813-10818 Protein denotes cIAP1
T6469 10823-10828 Protein denotes cIAP2
T6470 10836-10840 Regulation denotes role
T6471 10848-10859 Binding denotes recruitment
T6472 10863-10867 Protein denotes HOIP
T6473 10871-10876 Protein denotes TNFR1
T6474 10881-10885 Protein denotes CD40
T6475 10929-10940 Binding denotes association true
T6476 10944-10948 Protein denotes HOIP
T6477 10954-10958 Protein denotes CD40
T6478 10979-10986 Regulation denotes altered
T6479 11017-11026 Negative_regulation denotes inhibitor
T6480 11193-11200 Negative_regulation denotes reduced
T6481 11215-11220 Protein denotes cIAP1
T6482 11221-11231 Binding denotes associated
T6483 11398-11406 Negative_regulation denotes decrease true
T6484 11434-11438 Protein denotes HOIP
T6485 11457-11461 Protein denotes CD40
T6486 11637-11642 Regulation denotes alter
T6487 11677-11681 Protein denotes HOIP true
T6488 11767-11773 Regulation denotes alters
T6489 11797-11801 Protein denotes CD40 true
T6490 11802-11812 Binding denotes associated
T6491 11813-11817 Protein denotes HOIP true
T6492 11922-11926 Protein denotes cIAP
T6493 11936-11945 Regulation denotes influence
T6494 11950-11961 Binding denotes recruitment
T6495 11966-11997 Protein_modification denotes post-translational modification
T6496 12007-12011 Protein denotes CD40
T6497 12012-12022 Binding denotes associated
T6498 12023-12027 Protein denotes HOIP
T6499 14511-14515 Protein denotes HOIP
T6500 14519-14530 Positive_regulation denotes responsible
T6501 14539-14550 Binding denotes recruitment
T6502 14591-14595 Protein denotes CD40
T6503 14649-14653 Protein denotes HOIP
T6504 14654-14663 Negative_regulation denotes deficient
T6505 14668-14672 Protein denotes HOIP
T6506 14673-14686 Positive_regulation denotes reconstituted
T6507 14801-14805 Protein denotes HOIP
T6508 14817-14822 Protein denotes TRAF2
T6509 14824-14829 Protein denotes TRAF3
T6510 14831-14835 Protein denotes IKKα
T6511 14836-14837 Protein denotes β
T6512 14843-14847 Protein denotes IKKγ
T6513 14868-14873 Protein denotes TRAF2
T6514 14875-14880 Protein denotes TRAF3
T6515 14886-14891 Protein denotes cIAP1
T6516 14895-14899 Protein denotes CD40
T6517 14924-14928 Protein denotes HOIP
T6518 14929-14938 Negative_regulation denotes deficient
T6519 15011-15015 Protein denotes HOIP
T6520 15023-15031 Positive_regulation denotes required
T6521 15040-15051 Binding denotes association
T6522 15075-15079 Protein denotes CD40
T6523 15094-15098 Protein denotes IKKα
T6524 15099-15100 Protein denotes β
T6525 15105-15109 Protein denotes IKKγ
T6526 15133-15137 Protein denotes CD40
T6527 15172-15176 Protein denotes HOIP
T6528 15177-15186 Negative_regulation denotes deficient
T6529 15221-15225 Protein denotes IKKα
T6530 15226-15227 Protein denotes β
T6531 15232-15236 Protein denotes IKKγ
T6532 15240-15244 Protein denotes CD40
T6533 15269-15273 Protein denotes HOIP
T6534 15274-15287 Positive_regulation denotes reconstituted
T6535 15404-15415 Binding denotes recruitment
T6536 15457-15464 Negative_regulation denotes absence
T6537 15468-15472 Protein denotes HOIP
T6538 15473-15483 Gene_expression denotes expression
T6539 15510-15514 Protein denotes HOIP
T6540 15531-15542 Binding denotes recruitment
T6541 15651-15655 Protein denotes IKKγ
T6542 15667-15671 Protein denotes CD40
T6543 15830-15834 Protein denotes IKKγ
T6544 15964-15968 Protein denotes IKKγ
T6545 15987-15991 Protein denotes CD40
T6546 16229-16233 Protein denotes IKKγ
T6547 16298-16308 Gene_expression denotes expressing
T6548 16324-16328 Protein denotes IKKγ
T6549 16344-16355 Binding denotes recruitment
T6550 16359-16363 Protein denotes IKKγ
T6551 16367-16371 Protein denotes CD40
T6552 16372-16380 Positive_regulation denotes requires
T6553 16381-16385 Protein denotes HOIP
T6554 16429-16438 Positive_regulation denotes increased
T6555 16459-16463 Protein denotes IKKγ
T6556 16467-16471 Protein denotes CD40
T6557 16657-16664 Negative_regulation denotes reduced
T6558 16673-16677 Protein denotes CD40
T6559 16678-16688 Binding denotes associated
T6560 16689-16693 Protein denotes IKKγ
T6561 16906-16910 Protein denotes CD40
T6562 16911-16921 Binding denotes associated
T6563 16922-16926 Protein denotes IKKγ
T6564 16988-17003 Phosphorylation denotes phosphorylation
T6565 17042-17046 Protein denotes HOIP
T6566 17050-17058 Positive_regulation denotes required
T6567 17067-17078 Binding denotes association
T6568 17082-17086 Protein denotes IKKγ
T6569 17092-17096 Protein denotes CD40
T6570 17129-17136 Positive_regulation denotes coupled
T6571 17140-17158 Protein_modification denotes post-translational
T6572 17159-17172 Negative_regulation denotes modifications
T6573 17176-17180 Protein denotes IKKγ
T14759 2125-2129 Protein denotes HOIP
T14760 2161-2166 Protein denotes Rnf31
T14761 2202-2206 Protein denotes HOIP
T14762 2443-2448 Protein denotes Rnf31
T14763 2449-2457 Positive_regulation denotes resulted
T14764 2579-2583 Protein denotes HOIP
T14765 3016-3020 Protein denotes HOIP
T14766 3021-3030 Negative_regulation denotes deficient
T14767 3032-3036 Protein denotes HOIP
T14768 3059-3067 Negative_regulation denotes decrease
T14769 3071-3075 Protein denotes HOIP
T14770 3084-3094 Gene_expression denotes expression
T14771 3125-3135 Negative_regulation denotes disruption
T14772 3151-3156 Protein denotes Rnf31
T14773 3158-3162 Protein denotes HOIP
T14774 3168-3172 Protein denotes HOIP
T14775 3173-3183 Gene_expression denotes expression
T14776 3291-3295 Protein denotes HOIP
T14777 3343-3347 Protein denotes HOIP
T15355 4763-4767 Protein denotes CD40
T15356 4768-4776 Positive_regulation denotes mediated
T15357 4777-4781 Protein denotes CD80
T15358 4782-4794 Positive_regulation denotes upregulation
T15359 4798-4807 Negative_regulation denotes defective true
T15360 4811-4815 Protein denotes HOIP
T15361 4816-4825 Negative_regulation denotes deficient
T15362 4956-4966 Gene_expression denotes expression
T15363 4970-4974 Protein denotes CD80
T15364 5118-5122 Protein denotes HOIP
T15365 5123-5132 Negative_regulation denotes deficient
T15366 5134-5138 Protein denotes HOIP
T15367 5182-5186 Protein denotes HOIP
T15368 5196-5205 Negative_regulation denotes defective
T15369 5206-5210 Protein denotes CD80
T15370 5211-5223 Positive_regulation denotes upregulation
T15696 6765-6768 Protein denotes GLε
T15697 6769-6782 Transcription denotes transcription
T15698 6799-6803 Protein denotes CD40
T15699 6815-6819 Protein denotes HOIP
T15700 6820-6829 Negative_regulation denotes deficient
T15701 6848-6852 Protein denotes HOIP true
T15702 6853-6862 Negative_regulation denotes deficient
T15703 6931-6935 Protein denotes HOIP
T15704 6965-6969 Protein denotes HOIP
T15705 6970-6979 Negative_regulation denotes deficient
T15706 7004-7008 Protein denotes HOIP
T15707 7030-7034 Protein denotes HOIP
T15708 7040-7044 Protein denotes HOIP
T15709 7149-7153 Protein denotes IL-4
T15710 7271-7274 Protein denotes GLε
T15711 7329-7334 Protein denotes Hprt1
T15712 7335-7346 Transcription denotes transcripts
T15713 7561-7565 Protein denotes HOIP
T15714 7566-7575 Negative_regulation denotes deficient
T16141 9287-9291 Protein denotes CD40
T16142 9344-9348 Protein denotes HOIP
T16143 9349-9358 Negative_regulation denotes deficient
T16144 9366-9370 Protein denotes CD40
T16145 9407-9411 Protein denotes HOIP
T16146 9412-9421 Negative_regulation denotes deficient
T16147 9541-9545 Protein denotes HOIP
T16148 9546-9555 Negative_regulation denotes deficient
T16149 9607-9611 Protein denotes HOIP
T16150 9613-9617 Protein denotes HOIP
T16151 9623-9627 Protein denotes HOIP
T16152 9656-9661 Protein denotes CD154
T16153 9662-9672 Gene_expression denotes expressing
T16154 9774-9781 Negative_regulation denotes lacking
T16155 9782-9787 Protein denotes CD154
T16156 9895-9907 Protein denotes Phospho-IκBα
T16157 9909-9914 Protein denotes pIκBα
T16158 9982-9986 Protein denotes IκBα
T16159 10069-10073 Protein denotes IκBα
T16160 10287-10291 Protein denotes HOIP
T16161 10292-10301 Negative_regulation denotes deficient
T16721 12077-12088 Binding denotes recruitment
T16722 12135-12139 Protein denotes HOIP
T16723 12140-12149 Negative_regulation denotes deficient
T16724 12184-12191 Negative_regulation denotes reduces true
T16725 12192-12203 Binding denotes recruitment
T16726 12207-12212 Protein denotes cIAP1
T16727 12216-12220 Protein denotes CD40
T16728 12229-12235 Regulation denotes modify
T16729 12236-12240 Protein denotes HOIP true
T16730 12241-12252 Binding denotes recruitment
T16731 12847-12852 Protein denotes cIAP1
T16732 12854-12859 Protein denotes TRAF2
T16733 12861-12866 Protein denotes TRAF3
T16734 12872-12876 Protein denotes HOIP
T16735 12993-12997 Protein denotes CD40
T16736 13068-13072 Protein denotes HOIP
T16737 13073-13082 Negative_regulation denotes deficient
T16738 13171-13175 Protein denotes HOIP
T16739 13307-13311 Protein denotes CD40
T16740 13313-13318 Protein denotes TRAF2
T16741 13320-13325 Protein denotes TRAF3
T16742 13327-13332 Protein denotes cIAP1
T16743 13334-13338 Protein denotes HOIP
T16744 13340-13344 Protein denotes IKKα
T16745 13345-13346 Protein denotes β
T16746 13352-13356 Protein denotes IKKγ
T16747 13373-13377 Protein denotes HOIP
T16748 13378-13388 Gene_expression denotes expression
T16749 13393-13401 Positive_regulation denotes required
T16750 13443-13447 Protein denotes CD40
T16751 13606-13610 Protein denotes HOIP
T16752 13611-13620 Negative_regulation denotes deficient
T16753 13652-13656 Protein denotes HOIP
T16754 13657-13666 Regulation denotes dependent
T16755 13667-13678 Binding denotes recruitment
T16756 13682-13686 Protein denotes IKKγ
T16757 13690-13694 Protein denotes CD40
T16758 13712-13716 Protein denotes HOIP
T16759 13717-13726 Negative_regulation denotes deficient
T16760 13728-13732 Protein denotes HOIP
T16761 13851-13855 Protein denotes IKKγ
T16762 13880-13884 Protein denotes pMIP
T16763 13889-13893 Protein denotes IKKγ
T16764 14095-14099 Protein denotes IKKγ
T16765 14118-14123 Protein denotes TRAF2
T16766 14383-14388 Protein denotes TRAF3
T16767 14393-14397 Protein denotes HOIP
R2078 T2762 T2763 themeOf HOIP,deficient
R2079 T2765 T2764 themeOf Rnf31,disruption
R2080 T2766 T2765 equivalentTo HOIP,Rnf31
R2081 T2767 T2768 themeOf HOIP,recruited
R2082 T2770 T2769 themeOf CD40,interaction
R2083 T2771 T2769 themeOf HOIP,interaction
R2084 T2772 T2776 themeOf recruitment,dependent
R2085 T2773 T2772 themeOf HOIP,recruitment
R2086 T2774 T2772 themeOf CD40,recruitment
R2087 T2775 T2776 causeOf TRAF2,dependent
R2088 T2784 T2783 themeOf HOIP,disrupt
R2089 T2787 T2786 equivalentTo HOIP,Rnf31
R2090 T2789 T2788 themeOf Rnf31,disrupted
R2091 T2791 T2790 themeOf Rnf31,disrupted
R2092 T2793 T2794 themeOf Rnf31,disrupted
R2093 T2795 T2796 themeOf HOIP,expression
R2094 T2797 T2798 themeOf HOIP,deficient
R2095 T2799 T2800 themeOf HOIP,deficient
R2096 T2802 T2801 themeOf Rnf31,disruption
R2097 T2803 T2804 themeOf HOIP,deficient
R2098 T2807 T2808 themeOf HOIP,expression
R2099 T2808 T2806 themeOf expression,restoring
R2100 T2810 T2811 themeOf HOIP,deficient
R3163 T4105 T4106 causeOf HOIP,required
R3164 T4107 T4108 causeOf CD40,mediated
R3165 T4108 T4106 themeOf mediated,required
R3166 T4109 T4110 themeOf CD80,upregulation
R3167 T4110 T4108 themeOf upregulation,mediated
R3168 T4112 T4114 causeOf Engagement,upregulates
R3169 T4113 T4112 themeOf CD40,Engagement
R3170 T4115 T4114 themeOf expression,upregulates
R3171 T4116 T4115 themeOf CD80,expression
R3172 T4119 T4121 themeOf upregulation,induced
R3173 T4120 T4119 themeOf CD80,upregulation
R3174 T4122 T4121 causeOf engagement,induced
R3175 T4123 T4122 themeOf CD40,engagement
R3176 T4124 T4125 themeOf HOIP,deficient
R3177 T4126 T4127 themeOf HOIP,reconstituted
R3178 T4128 T4129 causeOf CD40,stimulated
R3179 T4129 T4132 themeOf stimulated,reduced
R3180 T4130 T4129 themeOf upregulation,stimulated
R3181 T4130 T4132 themeOf upregulation,reduced
R3182 T4131 T4130 themeOf CD80,upregulation
R3183 T4133 T4134 themeOf HOIP,deficient
R3184 T4134 T4132 causeOf deficient,reduced
R3185 T4136 T4135 themeOf CD80,upregulation
R3186 T4137 T4138 themeOf HOIP,reconstituted
R3187 T4139 T4144 themeOf defect,due
R3188 T4140 T4141 themeOf CD80,upregulation
R3189 T4141 T4144 themeOf upregulation,due
R3190 T4141 T4139 themeOf upregulation,defect
R3191 T4142 T4143 themeOf HOIP,deficient
R3192 T4143 T4139 causeOf deficient,defect
R3193 T4145 T4144 causeOf disruption,due
R3194 T4145 T4139 causeOf disruption,defect
R3195 T4146 T4145 themeOf Rnf31,disruption
R3196 T4155 T4154 themeOf germline epsilon,production
R3197 T4156 T4155 equivalentTo GLε,germline epsilon
R3198 T4157 T4158 causeOf IL-4,induced
R3199 T4159 T4160 themeOf GLε,transcription
R3200 T4160 T4158 themeOf transcription,induced
R3201 T4161 T4162 themeOf GLε,expression
R3202 T4163 T4164 themeOf HOIP,deficient
R3203 T4166 T4167 themeOf GLε,expression
R3204 T4167 T4168 themeOf expression,induced
R3205 T4169 T4170 themeOf HOIP,reconstituted
R3206 T4170 T4168 causeOf reconstituted,induced
R4169 T5430 T5432 themeOf CD154,expressed
R4170 T5431 T5430 equivalentTo CD40 ligand,CD154
R4171 T5436 T5438 causeOf Phosphorylation,allowing
R4172 T5440 T5441 causeOf CD40,mediated
R4173 T5441 T5447 themeOf mediated,impaired
R4174 T5442 T5441 themeOf phosphorylation,mediated
R4175 T5443 T5441 themeOf degradation,mediated
R4176 T5444 T5443 themeOf IκBα,degradation
R4177 T5444 T5442 themeOf IκBα,phosphorylation
R4178 T5445 T5446 themeOf HOIP,deficient
R4179 T5448 T5449 themeOf CD40,engagement
R4180 T5451 T5452 themeOf HOIP,deficient
R4181 T5455 T5456 themeOf HOIP,reconstituted
R4182 T5459 T5460 themeOf HOIP,expression
R4183 T5460 T5458 themeOf expression,absence
R4932 T6456 T6457 themeOf HOIP,deficient
R4933 T6459 T6460 themeOf HOIP,recruited
R4934 T6460 T6462 themeOf recruited,dependent
R4935 T6461 T6462 causeOf TRAF2,dependent
R4936 T6466 T6470 causeOf TRAF2,role
R4937 T6466 T6467 themeOf TRAF2,associated
R4938 T6467 T6470 causeOf associated,role
R4939 T6468 T6470 causeOf cIAP1,role
R4940 T6468 T6467 themeOf cIAP1,associated
R4941 T6469 T6467 themeOf cIAP2,associated
R4942 T6469 T6470 causeOf cIAP2,role
R4943 T6471 T6470 themeOf recruitment,role
R4944 T6472 T6471 themeOf HOIP,recruitment
R4945 T6473 T6471 themeOf TNFR1,recruitment
R4946 T6474 T6471 themeOf CD40,recruitment
R4947 T6475 T6478 themeOf association,altered
R4948 T6476 T6475 themeOf HOIP,association
R4949 T6477 T6475 themeOf CD40,association
R4950 T6479 T6478 causeOf inhibitor,altered
R4951 T6481 T6482 themeOf cIAP1,associated
R4952 T6482 T6480 themeOf associated,reduced
R4953 T6484 T6483 themeOf HOIP,decrease
R4954 T6489 T6490 themeOf CD40,associated
R4955 T6490 T6488 themeOf associated,alters
R4956 T6491 T6490 themeOf HOIP,associated
R4957 T6491 T6488 themeOf HOIP,alters
R4958 T6492 T6493 causeOf cIAP,influence
R4959 T6494 T6493 themeOf recruitment,influence
R4960 T6495 T6493 themeOf post-translational modification,influence
R4961 T6496 T6497 themeOf CD40,associated
R4962 T6496 T6495 themeOf CD40,post-translational modification
R4963 T6498 T6495 themeOf HOIP,post-translational modification
R4964 T6498 T6494 themeOf HOIP,recruitment
R4965 T6498 T6497 themeOf HOIP,associated
R4966 T6499 T6500 causeOf HOIP,responsible
R4967 T6501 T6500 themeOf recruitment,responsible
R4968 T6502 T6501 themeOf CD40,recruitment
R4969 T6503 T6504 themeOf HOIP,deficient
R4970 T6505 T6506 themeOf HOIP,reconstituted
R4971 T6513 T6521 themeOf TRAF2,association
R4972 T6514 T6521 themeOf TRAF3,association
R4973 T6515 T6521 themeOf cIAP1,association
R4974 T6517 T6518 themeOf HOIP,deficient
R4975 T6519 T6520 causeOf HOIP,required
R4976 T6519 T6521 themeOf HOIP,association
R4977 T6521 T6520 themeOf association,required
R4978 T6522 T6521 themeOf CD40,association
R4979 T6527 T6528 themeOf HOIP,deficient
R4980 T6533 T6534 themeOf HOIP,reconstituted
R4981 T6537 T6538 themeOf HOIP,expression
R4982 T6538 T6536 themeOf expression,absence
R4983 T6548 T6547 themeOf IKKγ,expressing
R4984 T6549 T6552 themeOf recruitment,requires
R4985 T6550 T6549 themeOf IKKγ,recruitment
R4986 T6551 T6549 themeOf CD40,recruitment
R4987 T6555 T6554 themeOf IKKγ,increased
R4988 T6556 T6554 themeOf CD40,increased
R4989 T6558 T6559 themeOf CD40,associated
R4990 T6559 T6557 themeOf associated,reduced
R4991 T6560 T6559 themeOf IKKγ,associated
R4992 T6560 T6557 themeOf IKKγ,reduced
R4993 T6561 T6562 themeOf CD40,associated
R4994 T6563 T6562 themeOf IKKγ,associated
R4995 T6565 T6566 causeOf HOIP,required
R4996 T6567 T6566 themeOf association,required
R4997 T6568 T6567 themeOf IKKγ,association
R4998 T6569 T6567 themeOf CD40,association
R4999 T6571 T6570 themeOf post-translational,coupled
R5000 T6573 T6571 themeOf IKKγ,post-translational
R11690 T14765 T14766 themeOf HOIP,deficient
R11691 T14769 T14770 themeOf HOIP,expression
R11692 T14770 T14768 themeOf expression,decrease
R11693 T14771 T14768 causeOf disruption,decrease
R11694 T14773 T14772 equivalentTo HOIP,Rnf31
R11695 T14774 T14775 themeOf HOIP,expression
R12159 T15355 T15356 causeOf CD40,mediated
R12160 T15356 T15359 themeOf mediated,defective
R12161 T15357 T15358 themeOf CD80,upregulation
R12162 T15358 T15356 themeOf upregulation,mediated
R12163 T15358 T15359 themeOf upregulation,defective
R12164 T15360 T15361 themeOf HOIP,deficient
R12165 T15361 T15359 causeOf deficient,defective
R12166 T15363 T15362 themeOf CD80,expression
R12167 T15364 T15365 themeOf HOIP,deficient
R12168 T15369 T15370 themeOf CD80,upregulation
R12169 T15370 T15368 themeOf upregulation,defective
R12415 T15696 T15697 themeOf GLε,transcription
R12416 T15699 T15700 themeOf HOIP,deficient
R12417 T15701 T15702 themeOf HOIP,deficient
R12418 T15704 T15705 themeOf HOIP,deficient
R12419 T15711 T15712 themeOf Hprt1,transcripts
R12420 T15713 T15714 themeOf HOIP,deficient
R12742 T16142 T16143 themeOf HOIP,deficient
R12743 T16145 T16146 themeOf HOIP,deficient
R12744 T16147 T16148 themeOf HOIP,deficient
R12745 T16150 T16149 equivalentTo HOIP,HOIP
R12746 T16152 T16153 themeOf CD154,expressing
R12747 T16155 T16154 themeOf CD154,lacking
R12748 T16157 T16156 equivalentTo pIκBα,Phospho-IκBα
R12749 T16160 T16161 themeOf HOIP,deficient
R13153 T16722 T16723 themeOf HOIP,deficient
R13154 T16725 T16724 themeOf recruitment,reduces
R13155 T16725 T16728 causeOf recruitment,modify
R13156 T16726 T16725 themeOf cIAP1,recruitment
R13157 T16727 T16725 themeOf CD40,recruitment
R13158 T16729 T16730 themeOf HOIP,recruitment
R13159 T16730 T16728 themeOf recruitment,modify
R13160 T16736 T16737 themeOf HOIP,deficient
R13161 T16747 T16748 themeOf HOIP,expression
R13162 T16748 T16749 causeOf expression,required
R13163 T16751 T16752 themeOf HOIP,deficient
R13164 T16753 T16754 causeOf HOIP,dependent
R13165 T16755 T16754 themeOf recruitment,dependent
R13166 T16756 T16755 themeOf IKKγ,recruitment
R13167 T16757 T16755 themeOf CD40,recruitment
R13168 T16758 T16759 themeOf HOIP,deficient