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PMC:3148254 / 33092-34299 JSONTXT

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bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T14274 44-48 Protein denotes CD40

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T14732 44-48 Protein denotes CD40
T14733 290-302 Protein denotes anti-rat IgG
T14734 304-311 Protein denotes Jackson
T14735 329-333 Protein denotes CD40
T14736 335-339 Protein denotes 1C10
T14737 623-641 Protein denotes lambda phosphatase
T14738 601-617 Phosphorylation denotes dephosphorylated
R11682 T14737 T14738 themeOf lambda phosphatase,dephosphorylated

events-check-again

Id Subject Object Predicate Lexical cue
T14739 44-48 Protein denotes CD40

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T14502 0-20 NN denotes Immunoprecipitations
T14503 21-40 NN denotes Immunoprecipitation
T14504 41-43 IN denotes of
T14505 44-48 NN denotes CD40
T14506 49-52 VB denotes was
T14507 53-62 VB denotes performed
T14508 63-65 IN denotes by
T14509 66-75 VB denotes activated
T14510 76-84 NN denotes receptor
T14511 85-92 NN denotes capture
T14512 92-93 -COMMA- denotes ,
T14513 94-96 IN denotes as
T14514 97-107 RB denotes previously
T14515 108-117 VB denotes described
T14516 118-119 -LRB- denotes [
T14517 119-120 CD denotes 6
T14518 120-121 -RRB- denotes ]
T14519 123-130 NNP denotes Briefly
T14520 130-131 -COMMA- denotes ,
T14521 132-137 CD denotes 3×107
T14522 138-143 NN denotes cells
T14523 144-145 -LRB- denotes (
T14524 145-151 NN denotes A20.2J
T14525 152-155 CC denotes and
T14526 156-167 NN denotes derivatives
T14527 167-168 -RRB- denotes )
T14528 169-173 VB denotes were
T14529 174-183 VB denotes incubated
T14530 184-187 IN denotes for
T14531 188-190 CD denotes 60
T14532 191-198 NN denotes minutes
T14533 199-201 IN denotes at
T14534 202-206 NN denotes room
T14535 207-218 NN denotes temperature
T14536 219-223 IN denotes with
T14537 224-226 CD denotes 10
T14538 227-229 NN denotes µl
T14539 230-238 JJ denotes magnetic
T14540 239-246 NN denotes protein
T14541 247-248 NN denotes G
T14542 249-254 NN denotes beads
T14543 255-256 -LRB- denotes (
T14544 256-261 JJ denotes Dynal
T14545 261-262 -RRB- denotes )
T14546 263-273 JJ denotes pre-coated
T14547 274-278 IN denotes with
T14548 279-281 CD denotes 10
T14549 282-284 NN denotes µg
T14550 285-289 NN denotes goat
T14551 290-298 JJ denotes anti-rat
T14552 299-302 NN denotes IgG
T14553 303-304 -LRB- denotes (
T14554 304-311 NN denotes Jackson
T14555 311-312 -RRB- denotes )
T14556 312-313 -COMMA- denotes ,
T14557 314-317 CC denotes and
T14558 318-320 CD denotes 10
T14559 321-323 NN denotes µg
T14560 324-333 NN denotes anti-CD40
T14561 334-335 -LRB- denotes (
T14562 335-339 NN denotes 1C10
T14563 339-340 -RRB- denotes )
T14564 341-343 CC denotes or
T14565 344-346 DT denotes an
T14566 347-354 NN denotes isotype
T14567 355-362 NN denotes control
T14568 363-371 NN denotes antibody
T14569 372-373 -LRB- denotes (
T14570 373-378 NN denotes mAb72
T14571 378-379 -RRB- denotes )
T14572 381-392 NN denotes Cells/beads
T14573 393-397 VB denotes were
T14574 398-402 RB denotes then
T14575 403-411 VB denotes pelleted
T14576 412-414 IN denotes by
T14577 415-429 NN denotes centrifugation
T14578 430-433 CC denotes and
T14579 434-439 VB denotes lysed
T14580 440-442 IN denotes in
T14581 443-449 NN denotes buffer
T14582 450-460 VB denotes containing
T14583 461-462 CD denotes 1
T14584 462-463 NN denotes %
T14585 464-470 NN denotes Triton
T14586 471-475 NN denotes X100
T14587 477-482 NN denotes Beads
T14588 483-487 VB denotes were
T14589 488-494 VB denotes washed
T14590 495-499 IN denotes with
T14591 500-505 NN denotes lysis
T14592 506-512 NN denotes buffer
T14593 513-515 TO denotes to
T14594 516-522 VB denotes remove
T14595 523-530 JJ denotes unbound
T14596 531-539 NN denotes material
T14597 541-543 IN denotes In
T14598 544-548 DT denotes some
T14599 549-560 NN denotes experiments
T14600 560-561 -COMMA- denotes ,
T14601 562-570 NN denotes material
T14602 571-581 VB denotes associated
T14603 582-586 IN denotes with
T14604 587-590 DT denotes the
T14605 591-596 NN denotes beads
T14606 597-600 VB denotes was
T14607 601-617 VB denotes dephosphorylated
T14608 618-622 IN denotes with
T14609 623-629 NN denotes lambda
T14610 630-641 NN denotes phosphatase
T14611 642-643 -LRB- denotes (
T14612 643-646 NNP denotes New
T14613 647-654 NNP denotes England
T14614 655-662 NNP denotes BioLabs
T14615 662-663 -RRB- denotes )
T14616 664-666 IN denotes as
T14617 667-670 IN denotes per
T14618 671-683 NN denotes manufacturer
T14619 683-685 POS denotes 's
T14620 686-698 NN denotes instructions
T14621 700-705 NN denotes Beads
T14622 706-710 VB denotes were
T14623 711-722 VB denotes resuspended
T14624 723-725 IN denotes in
T14625 726-728 NN denotes 2X
T14626 729-737 NN denotes SDS-PAGE
T14627 738-744 NN denotes sample
T14628 745-751 NN denotes buffer
T14629 752-755 CC denotes and
T14630 756-762 VB denotes heated
T14631 763-766 IN denotes for
T14632 767-768 CD denotes 5
T14633 769-776 NN denotes minutes
T14634 777-779 IN denotes at
T14635 780-785 NNP denotes 95°C.
T14636 786-794 NNP denotes Material
T14637 795-801 VB denotes eluted
T14638 802-806 IN denotes from
T14639 807-810 DT denotes the
T14640 811-816 NN denotes beads
T14641 817-820 VB denotes was
T14642 821-833 VB denotes fractionated
T14643 834-836 IN denotes by
T14644 837-845 NN denotes SDS-PAGE
T14645 846-849 CC denotes and
T14646 850-861 VB denotes transferred
T14647 862-864 TO denotes to
T14648 865-869 NN denotes PVDF
T14649 870-879 NN denotes membranes
T14650 880-883 IN denotes for
T14651 884-891 JJ denotes Western
T14652 892-900 NN denotes blotting
T14653 902-904 IN denotes In
T14654 905-909 DT denotes some
T14655 910-921 NN denotes experiments
T14656 921-922 -COMMA- denotes ,
T14657 923-928 NN denotes cells
T14658 929-933 VB denotes were
T14659 934-942 VB denotes cultured
T14660 943-946 IN denotes for
T14661 947-948 CD denotes 6
T14662 949-952 NN denotes hrs
T14663 953-957 IN denotes with
T14664 958-960 CD denotes 25
T14665 961-963 NN denotes µM
T14666 964-983 JJ denotes antennapedia-linked
T14667 984-991 NN denotes SMAC-N7
T14668 992-999 NN denotes peptide
T14669 1000-1001 -LRB- denotes (
T14670 1001-1011 NN denotes Calbiochem
T14671 1011-1012 -RRB- denotes )
T14672 1013-1015 CC denotes or
T14673 1016-1018 DT denotes an
T14674 1019-1030 JJ denotes appropriate
T14675 1031-1037 NN denotes volume
T14676 1038-1040 IN denotes of
T14677 1041-1044 DT denotes the
T14678 1045-1052 NN denotes solvent
T14679 1053-1057 VB denotes used
T14680 1058-1061 IN denotes for
T14681 1062-1065 DT denotes the
T14682 1066-1073 NN denotes peptide
T14683 1074-1075 -LRB- denotes (
T14684 1075-1079 NN denotes DMSO
T14685 1079-1080 -RRB- denotes )
T14686 1082-1087 IN denotes After
T14687 1088-1098 NN denotes incubation
T14688 1098-1099 -COMMA- denotes ,
T14689 1100-1105 NN denotes cells
T14690 1106-1107 -LRB- denotes (
T14691 1107-1109 IN denotes in
T14692 1110-1118 JJ denotes peptide-
T14693 1119-1121 CC denotes or
T14694 1122-1137 JJ denotes DMSO-containing
T14695 1138-1144 NN denotes medium
T14696 1144-1145 -RRB- denotes )
T14697 1146-1150 VB denotes were
T14698 1151-1161 VB denotes stimulated
T14699 1162-1166 IN denotes with
T14700 1167-1182 JJ denotes antibody-coated
T14701 1183-1188 NN denotes beads
T14702 1189-1191 IN denotes as
T14703 1192-1200 VB denotes outlined
T14704 1201-1206 IN denotes above
R11475 T14503 T14504 arg1Of Immunoprecipitation,of
R11476 T14503 T14506 arg1Of Immunoprecipitation,was
R11477 T14503 T14507 arg2Of Immunoprecipitation,performed
R11478 T14505 T14504 arg2Of CD40,of
R11479 T14507 T14506 arg2Of performed,was
R11480 T14511 T14507 arg1Of capture,performed
R11481 T14511 T14508 arg2Of capture,by
R11482 T14511 T14509 arg2Of capture,activated
R11483 T14511 T14510 arg1Of capture,receptor
R11484 T14511 T14512 arg1Of capture,","
R11485 T14511 T14513 arg1Of capture,as
R11486 T14515 T14513 arg2Of described,as
R11487 T14515 T14514 arg1Of described,previously
R11488 T14515 T14516 arg1Of described,[
R11489 T14517 T14516 arg2Of 6,[
R11490 T14518 T14516 arg3Of ],[
R11491 T14519 T14520 arg1Of Briefly,","
R11492 T14519 T14528 arg1Of Briefly,were
R11493 T14519 T14529 arg2Of Briefly,incubated
R11494 T14522 T14520 arg2Of cells,","
R11495 T14522 T14521 arg1Of cells,3×107
R11496 T14522 T14523 arg1Of cells,(
R11497 T14524 T14525 arg1Of A20.2J,and
R11498 T14525 T14523 arg2Of and,(
R11499 T14526 T14525 arg2Of derivatives,and
R11500 T14527 T14523 arg3Of ),(
R11501 T14529 T14528 arg2Of incubated,were
R11502 T14529 T14530 arg1Of incubated,for
R11503 T14532 T14531 arg1Of minutes,60
R11504 T14532 T14533 arg1Of minutes,at
R11505 T14532 T14536 arg1Of minutes,with
R11506 T14532 T14564 arg1Of minutes,or
R11507 T14535 T14533 arg2Of temperature,at
R11508 T14535 T14534 arg1Of temperature,room
R11509 T14542 T14536 arg2Of beads,with
R11510 T14542 T14537 arg1Of beads,10
R11511 T14542 T14538 arg1Of beads,µl
R11512 T14542 T14539 arg1Of beads,magnetic
R11513 T14542 T14540 arg1Of beads,protein
R11514 T14542 T14541 arg1Of beads,G
R11515 T14542 T14543 arg1Of beads,(
R11516 T14542 T14546 arg1Of beads,pre-coated
R11517 T14544 T14543 arg2Of Dynal,(
R11518 T14545 T14543 arg3Of ),(
R11519 T14546 T14547 arg1Of pre-coated,with
R11520 T14552 T14548 arg1Of IgG,10
R11521 T14552 T14549 arg1Of IgG,µg
R11522 T14552 T14550 arg1Of IgG,goat
R11523 T14552 T14551 arg1Of IgG,anti-rat
R11524 T14552 T14553 arg1Of IgG,(
R11525 T14552 T14557 arg1Of IgG,and
R11526 T14554 T14553 arg2Of Jackson,(
R11527 T14555 T14553 arg3Of ),(
R11528 T14557 T14547 arg2Of and,with
R11529 T14557 T14556 arg1Of and,","
R11530 T14560 T14557 arg2Of anti-CD40,and
R11531 T14560 T14558 arg1Of anti-CD40,10
R11532 T14560 T14559 arg1Of anti-CD40,µg
R11533 T14560 T14561 arg1Of anti-CD40,(
R11534 T14562 T14561 arg2Of 1C10,(
R11535 T14563 T14561 arg3Of ),(
R11536 T14564 T14530 arg2Of or,for
R11537 T14568 T14564 arg2Of antibody,or
R11538 T14568 T14565 arg1Of antibody,an
R11539 T14568 T14566 arg1Of antibody,isotype
R11540 T14568 T14567 arg1Of antibody,control
R11541 T14568 T14569 arg1Of antibody,(
R11542 T14570 T14569 arg2Of mAb72,(
R11543 T14571 T14569 arg3Of ),(
R11544 T14572 T14573 arg1Of Cells/beads,were
R11545 T14572 T14575 arg2Of Cells/beads,pelleted
R11546 T14572 T14579 arg2Of Cells/beads,lysed
R11547 T14575 T14578 arg1Of pelleted,and
R11548 T14577 T14575 arg1Of centrifugation,pelleted
R11549 T14577 T14576 arg2Of centrifugation,by
R11550 T14578 T14573 arg2Of and,were
R11551 T14578 T14574 arg1Of and,then
R11552 T14579 T14578 arg2Of lysed,and
R11553 T14579 T14580 arg1Of lysed,in
R11554 T14581 T14580 arg2Of buffer,in
R11555 T14581 T14582 arg1Of buffer,containing
R11556 T14583 T14584 arg1Of 1,%
R11557 T14586 T14582 arg2Of X100,containing
R11558 T14586 T14583 arg1Of X100,1
R11559 T14586 T14585 arg1Of X100,Triton
R11560 T14587 T14588 arg1Of Beads,were
R11561 T14587 T14589 arg2Of Beads,washed
R11562 T14589 T14588 arg2Of washed,were
R11563 T14589 T14590 arg1Of washed,with
R11564 T14589 T14593 modOf washed,to
R11565 T14592 T14590 arg2Of buffer,with
R11566 T14592 T14591 arg1Of buffer,lysis
R11567 T14594 T14593 arg1Of remove,to
R11568 T14596 T14594 arg2Of material,remove
R11569 T14596 T14595 arg1Of material,unbound
R11570 T14599 T14597 arg2Of experiments,In
R11571 T14599 T14598 arg1Of experiments,some
R11572 T14601 T14602 arg2Of material,associated
R11573 T14601 T14606 arg1Of material,was
R11574 T14601 T14607 arg2Of material,dephosphorylated
R11575 T14602 T14603 arg1Of associated,with
R11576 T14605 T14603 arg2Of beads,with
R11577 T14605 T14604 arg1Of beads,the
R11578 T14607 T14597 arg1Of dephosphorylated,In
R11579 T14607 T14600 arg1Of dephosphorylated,","
R11580 T14607 T14606 arg2Of dephosphorylated,was
R11581 T14607 T14608 arg1Of dephosphorylated,with
R11582 T14610 T14608 arg2Of phosphatase,with
R11583 T14610 T14609 arg1Of phosphatase,lambda
R11584 T14610 T14611 arg1Of phosphatase,(
R11585 T14610 T14617 arg1Of phosphatase,per
R11586 T14614 T14611 arg2Of BioLabs,(
R11587 T14614 T14612 arg1Of BioLabs,New
R11588 T14614 T14613 arg1Of BioLabs,England
R11589 T14615 T14611 arg3Of ),(
R11590 T14617 T14616 arg1Of per,as
R11591 T14618 T14619 arg2Of manufacturer,'s
R11592 T14620 T14617 arg2Of instructions,per
R11593 T14620 T14619 arg1Of instructions,'s
R11594 T14621 T14622 arg1Of Beads,were
R11595 T14621 T14623 arg2Of Beads,resuspended
R11596 T14621 T14630 arg2Of Beads,heated
R11597 T14623 T14624 arg1Of resuspended,in
R11598 T14623 T14629 arg1Of resuspended,and
R11599 T14628 T14624 arg2Of buffer,in
R11600 T14628 T14625 arg1Of buffer,2X
R11601 T14628 T14626 arg1Of buffer,SDS-PAGE
R11602 T14628 T14627 arg1Of buffer,sample
R11603 T14629 T14622 arg2Of and,were
R11604 T14630 T14629 arg2Of heated,and
R11605 T14630 T14631 arg1Of heated,for
R11606 T14633 T14632 arg1Of minutes,5
R11607 T14633 T14634 arg1Of minutes,at
R11608 T14633 T14641 arg1Of minutes,was
R11609 T14633 T14642 arg2Of minutes,fractionated
R11610 T14633 T14646 arg2Of minutes,transferred
R11611 T14636 T14634 arg2Of Material,at
R11612 T14636 T14635 arg1Of Material,95°C.
R11613 T14636 T14637 arg2Of Material,eluted
R11614 T14637 T14638 arg1Of eluted,from
R11615 T14640 T14638 arg2Of beads,from
R11616 T14640 T14639 arg1Of beads,the
R11617 T14642 T14645 arg1Of fractionated,and
R11618 T14644 T14642 arg1Of SDS-PAGE,fractionated
R11619 T14644 T14643 arg2Of SDS-PAGE,by
R11620 T14645 T14622 modOf and,were
R11621 T14645 T14641 arg2Of and,was
R11622 T14646 T14645 arg2Of transferred,and
R11623 T14646 T14647 arg1Of transferred,to
R11624 T14646 T14650 arg1Of transferred,for
R11625 T14649 T14647 arg2Of membranes,to
R11626 T14649 T14648 arg1Of membranes,PVDF
R11627 T14652 T14650 arg2Of blotting,for
R11628 T14652 T14651 arg1Of blotting,Western
R11629 T14655 T14653 arg2Of experiments,In
R11630 T14655 T14654 arg1Of experiments,some
R11631 T14657 T14658 arg1Of cells,were
R11632 T14657 T14659 arg2Of cells,cultured
R11633 T14659 T14653 arg1Of cultured,In
R11634 T14659 T14656 arg1Of cultured,","
R11635 T14659 T14658 arg2Of cultured,were
R11636 T14659 T14660 arg1Of cultured,for
R11637 T14662 T14660 arg2Of hrs,for
R11638 T14662 T14661 arg1Of hrs,6
R11639 T14662 T14663 arg1Of hrs,with
R11640 T14668 T14664 arg1Of peptide,25
R11641 T14668 T14665 arg1Of peptide,µM
R11642 T14668 T14666 arg1Of peptide,antennapedia-linked
R11643 T14668 T14667 arg1Of peptide,SMAC-N7
R11644 T14668 T14669 arg1Of peptide,(
R11645 T14668 T14672 arg1Of peptide,or
R11646 T14670 T14669 arg2Of Calbiochem,(
R11647 T14671 T14669 arg3Of ),(
R11648 T14672 T14663 arg2Of or,with
R11649 T14675 T14672 arg2Of volume,or
R11650 T14675 T14673 arg1Of volume,an
R11651 T14675 T14674 arg1Of volume,appropriate
R11652 T14675 T14676 arg1Of volume,of
R11653 T14678 T14676 arg2Of solvent,of
R11654 T14678 T14677 arg1Of solvent,the
R11655 T14678 T14679 arg2Of solvent,used
R11656 T14679 T14680 arg1Of used,for
R11657 T14682 T14680 arg2Of peptide,for
R11658 T14682 T14681 arg1Of peptide,the
R11659 T14682 T14683 arg1Of peptide,(
R11660 T14684 T14683 arg2Of DMSO,(
R11661 T14685 T14683 arg3Of ),(
R11662 T14687 T14686 arg2Of incubation,After
R11663 T14689 T14690 arg1Of cells,(
R11664 T14689 T14697 arg1Of cells,were
R11665 T14689 T14698 arg2Of cells,stimulated
R11666 T14691 T14690 arg2Of in,(
R11667 T14692 T14693 arg1Of peptide-,or
R11668 T14694 T14693 arg2Of DMSO-containing,or
R11669 T14695 T14691 arg2Of medium,in
R11670 T14695 T14692 arg1Of medium,peptide-
R11671 T14695 T14694 arg1Of medium,DMSO-containing
R11672 T14696 T14690 arg3Of ),(
R11673 T14698 T14686 arg1Of stimulated,After
R11674 T14698 T14688 arg1Of stimulated,","
R11675 T14698 T14697 arg2Of stimulated,were
R11676 T14698 T14699 arg1Of stimulated,with
R11677 T14698 T14702 arg1Of stimulated,as
R11678 T14701 T14699 arg2Of beads,with
R11679 T14701 T14700 arg1Of beads,antibody-coated
R11680 T14703 T14702 arg2Of outlined,as
R11681 T14703 T14704 arg1Of outlined,above

2_test

Id Subject Object Predicate Lexical cue
21829693-20614026-89653809 119-120 20614026 denotes 6
T68192 119-120 20614026 denotes 6

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T14284 0-20 NNP denotes Immunoprecipitations
T14285 21-40 NNP denotes Immunoprecipitation
T14286 41-43 IN denotes of
T14287 44-48 NNP denotes CD40
T14288 49-52 VBD denotes was
T14289 53-62 VBN denotes performed
T14290 63-65 IN denotes by
T14291 66-75 VBN denotes activated
T14292 76-84 NN denotes receptor
T14293 85-92 NN denotes capture
T14294 92-93 , denotes ,
T14295 94-96 IN denotes as
T14296 97-107 RB denotes previously
T14297 108-117 VBN denotes described
T14298 118-119 NNP denotes [
T14299 119-120 CD denotes 6
T14300 120-121 NNP denotes ]
T14301 121-122 . denotes .
T14302 123-130 RB denotes Briefly
T14303 130-131 , denotes ,
T14304 132-133 CD denotes 3
T14305 133-134 NN denotes ×
T14306 134-137 CD denotes 107
T14307 138-143 NNS denotes cells
T14308 144-145 -LRB- denotes (
T14309 145-148 CD denotes A20
T14310 148-150 CD denotes .2
T14311 150-151 NNP denotes J
T14312 152-155 CC denotes and
T14313 156-167 NNS denotes derivatives
T14314 167-168 -RRB- denotes )
T14315 169-173 VBD denotes were
T14316 174-183 VBN denotes incubated
T14317 184-187 IN denotes for
T14318 188-190 CD denotes 60
T14319 191-198 NNS denotes minutes
T14320 199-201 IN denotes at
T14321 202-206 NN denotes room
T14322 207-218 NN denotes temperature
T14323 219-223 IN denotes with
T14324 224-226 CD denotes 10
T14325 227-229 NN denotes µl
T14326 230-238 JJ denotes magnetic
T14327 239-246 NN denotes protein
T14328 247-248 NNP denotes G
T14329 249-254 NNS denotes beads
T14330 255-256 -LRB- denotes (
T14331 256-261 NNP denotes Dynal
T14332 261-262 -RRB- denotes )
T14333 263-273 JJ denotes pre-coated
T14334 274-278 IN denotes with
T14335 279-281 CD denotes 10
T14336 282-284 NN denotes µg
T14337 285-289 NN denotes goat
T14338 290-298 JJ denotes anti-rat
T14339 299-302 NNP denotes IgG
T14340 303-304 -LRB- denotes (
T14341 304-311 NNP denotes Jackson
T14342 311-312 -RRB- denotes )
T14343 312-313 , denotes ,
T14344 314-317 CC denotes and
T14345 318-320 CD denotes 10
T14346 321-323 NN denotes µg
T14347 324-333 NN denotes anti-CD40
T14348 334-335 -LRB- denotes (
T14349 335-339 NNP denotes 1C10
T14350 339-340 -RRB- denotes )
T14351 341-343 CC denotes or
T14352 344-346 DT denotes an
T14353 347-354 NN denotes isotype
T14354 355-362 NN denotes control
T14355 363-371 NN denotes antibody
T14356 372-373 -LRB- denotes (
T14357 373-378 NNP denotes mAb72
T14358 378-379 -RRB- denotes )
T14359 379-380 . denotes .
T14360 381-392 NNS denotes Cells/beads
T14361 393-397 VBD denotes were
T14362 398-402 RB denotes then
T14363 403-411 VBN denotes pelleted
T14364 412-414 IN denotes by
T14365 415-429 NN denotes centrifugation
T14366 430-433 CC denotes and
T14367 434-439 VBN denotes lysed
T14368 440-442 IN denotes in
T14369 443-449 NN denotes buffer
T14370 450-460 VBG denotes containing
T14371 461-462 CD denotes 1
T14372 462-463 NN denotes %
T14373 464-470 NNP denotes Triton
T14374 471-475 NNP denotes X100
T14375 475-476 . denotes .
T14376 477-482 NNS denotes Beads
T14377 483-487 VBD denotes were
T14378 488-494 VBN denotes washed
T14379 495-499 IN denotes with
T14380 500-505 NN denotes lysis
T14381 506-512 NN denotes buffer
T14382 513-515 TO denotes to
T14383 516-522 VB denotes remove
T14384 523-530 JJ denotes unbound
T14385 531-539 NN denotes material
T14386 539-540 . denotes .
T14387 541-543 IN denotes In
T14388 544-548 DT denotes some
T14389 549-560 NNS denotes experiments
T14390 560-561 , denotes ,
T14391 562-570 NN denotes material
T14392 571-581 VBN denotes associated
T14393 582-586 IN denotes with
T14394 587-590 DT denotes the
T14395 591-596 NNS denotes beads
T14396 597-600 VBD denotes was
T14397 601-617 VBN denotes dephosphorylated
T14398 618-622 IN denotes with
T14399 623-629 JJ denotes lambda
T14400 630-641 NN denotes phosphatase
T14401 642-643 -LRB- denotes (
T14402 643-646 NNP denotes New
T14403 647-654 NNP denotes England
T14404 655-662 NNP denotes BioLabs
T14405 662-663 -RRB- denotes )
T14406 664-666 IN denotes as
T14407 667-670 IN denotes per
T14408 671-683 NN denotes manufacturer
T14409 683-685 POS denotes 's
T14410 686-698 NNS denotes instructions
T14411 698-699 . denotes .
T14412 700-705 NNS denotes Beads
T14413 706-710 VBD denotes were
T14414 711-722 VBN denotes resuspended
T14415 723-725 IN denotes in
T14416 726-728 NNP denotes 2X
T14417 729-737 NNP denotes SDS-PAGE
T14418 738-744 NN denotes sample
T14419 745-751 NN denotes buffer
T14420 752-755 CC denotes and
T14421 756-762 VBN denotes heated
T14422 763-766 IN denotes for
T14423 767-768 CD denotes 5
T14424 769-776 NNS denotes minutes
T14425 777-779 IN denotes at
T14426 780-782 CD denotes 95
T14427 782-783 CD denotes °
T14428 783-785 NNP denotes C.
T14429 786-794 NNP denotes Material
T14430 795-801 VBD denotes eluted
T14431 802-806 IN denotes from
T14432 807-810 DT denotes the
T14433 811-816 NNS denotes beads
T14434 817-820 VBD denotes was
T14435 821-833 VBN denotes fractionated
T14436 834-836 IN denotes by
T14437 837-845 NNP denotes SDS-PAGE
T14438 846-849 CC denotes and
T14439 850-861 VBN denotes transferred
T14440 862-864 TO denotes to
T14441 865-869 NNP denotes PVDF
T14442 870-879 NNS denotes membranes
T14443 880-883 IN denotes for
T14444 884-891 JJ denotes Western
T14445 892-900 VBG denotes blotting
T14446 900-901 . denotes .
T14447 902-904 IN denotes In
T14448 905-909 DT denotes some
T14449 910-921 NNS denotes experiments
T14450 921-922 , denotes ,
T14451 923-928 NNS denotes cells
T14452 929-933 VBD denotes were
T14453 934-942 VBN denotes cultured
T14454 943-946 IN denotes for
T14455 947-948 CD denotes 6
T14456 949-952 NNS denotes hrs
T14457 953-957 IN denotes with
T14458 958-960 CD denotes 25
T14459 961-963 NNP denotes µM
T14460 964-983 JJ denotes antennapedia-linked
T14461 984-991 NNP denotes SMAC-N7
T14462 992-999 NN denotes peptide
T14463 1000-1001 -LRB- denotes (
T14464 1001-1011 NNP denotes Calbiochem
T14465 1011-1012 -RRB- denotes )
T14466 1013-1015 CC denotes or
T14467 1016-1018 DT denotes an
T14468 1019-1030 JJ denotes appropriate
T14469 1031-1037 NN denotes volume
T14470 1038-1040 IN denotes of
T14471 1041-1044 DT denotes the
T14472 1045-1052 JJ denotes solvent
T14473 1053-1057 VBN denotes used
T14474 1058-1061 IN denotes for
T14475 1062-1065 DT denotes the
T14476 1066-1073 NN denotes peptide
T14477 1074-1075 -LRB- denotes (
T14478 1075-1079 NNP denotes DMSO
T14479 1079-1080 -RRB- denotes )
T14480 1080-1081 . denotes .
T14481 1082-1087 IN denotes After
T14482 1088-1098 NN denotes incubation
T14483 1098-1099 , denotes ,
T14484 1100-1105 NNS denotes cells
T14485 1106-1107 -LRB- denotes (
T14486 1107-1109 IN denotes in
T14487 1110-1117 NN denotes peptide
T14488 1117-1118 : denotes -
T14489 1119-1121 CC denotes or
T14490 1122-1137 JJ denotes DMSO-containing
T14491 1138-1144 NN denotes medium
T14492 1144-1145 -RRB- denotes )
T14493 1146-1150 VBD denotes were
T14494 1151-1161 VBN denotes stimulated
T14495 1162-1166 IN denotes with
T14496 1167-1182 JJ denotes antibody-coated
T14497 1183-1188 NNS denotes beads
T14498 1189-1191 IN denotes as
T14499 1192-1200 VBN denotes outlined
T14500 1201-1206 IN denotes above
T14501 1206-1207 . denotes .
R11257 T14284 T14285 compound Immunoprecipitations,Immunoprecipitation
R11258 T14285 T14289 nsubjpass Immunoprecipitation,performed
R11259 T14286 T14285 prep of,Immunoprecipitation
R11260 T14287 T14286 pobj CD40,of
R11261 T14288 T14289 auxpass was,performed
R11262 T14289 T14289 ROOT performed,performed
R11263 T14290 T14289 agent by,performed
R11264 T14291 T14293 amod activated,capture
R11265 T14292 T14293 compound receptor,capture
R11266 T14293 T14290 pobj capture,by
R11267 T14294 T14289 punct ",",performed
R11268 T14295 T14297 mark as,described
R11269 T14296 T14297 advmod previously,described
R11270 T14297 T14289 advcl described,performed
R11271 T14298 T14300 nmod [,]
R11272 T14299 T14300 nummod 6,]
R11273 T14300 T14297 dobj ],described
R11274 T14301 T14289 punct .,performed
R11275 T14302 T14316 advmod Briefly,incubated
R11276 T14303 T14316 punct ",",incubated
R11277 T14304 T14305 nummod 3,×
R11278 T14305 T14316 nsubjpass ×,incubated
R11279 T14306 T14307 nummod 107,cells
R11280 T14307 T14305 appos cells,×
R11281 T14308 T14307 punct (,cells
R11282 T14309 T14311 nummod A20,J
R11283 T14310 T14311 nummod .2,J
R11284 T14311 T14307 appos J,cells
R11285 T14312 T14311 cc and,J
R11286 T14313 T14311 conj derivatives,J
R11287 T14314 T14307 punct ),cells
R11288 T14315 T14316 auxpass were,incubated
R11289 T14316 T14316 ROOT incubated,incubated
R11290 T14317 T14316 prep for,incubated
R11291 T14318 T14319 nummod 60,minutes
R11292 T14319 T14317 pobj minutes,for
R11293 T14320 T14319 prep at,minutes
R11294 T14321 T14322 compound room,temperature
R11295 T14322 T14320 pobj temperature,at
R11296 T14323 T14316 prep with,incubated
R11297 T14324 T14325 nummod 10,µl
R11298 T14325 T14329 compound µl,beads
R11299 T14326 T14327 amod magnetic,protein
R11300 T14327 T14329 compound protein,beads
R11301 T14328 T14329 compound G,beads
R11302 T14329 T14323 pobj beads,with
R11303 T14330 T14329 punct (,beads
R11304 T14331 T14329 appos Dynal,beads
R11305 T14332 T14329 punct ),beads
R11306 T14333 T14316 advcl pre-coated,incubated
R11307 T14334 T14333 prep with,pre-coated
R11308 T14335 T14336 nummod 10,µg
R11309 T14336 T14339 compound µg,IgG
R11310 T14337 T14339 compound goat,IgG
R11311 T14338 T14339 compound anti-rat,IgG
R11312 T14339 T14334 pobj IgG,with
R11313 T14340 T14341 punct (,Jackson
R11314 T14341 T14339 appos Jackson,IgG
R11315 T14342 T14341 punct ),Jackson
R11316 T14343 T14339 punct ",",IgG
R11317 T14344 T14339 cc and,IgG
R11318 T14345 T14347 nummod 10,anti-CD40
R11319 T14346 T14347 compound µg,anti-CD40
R11320 T14347 T14339 conj anti-CD40,IgG
R11321 T14348 T14349 punct (,1C10
R11322 T14349 T14347 appos 1C10,anti-CD40
R11323 T14350 T14349 punct ),1C10
R11324 T14351 T14347 cc or,anti-CD40
R11325 T14352 T14355 det an,antibody
R11326 T14353 T14355 nmod isotype,antibody
R11327 T14354 T14355 compound control,antibody
R11328 T14355 T14347 conj antibody,anti-CD40
R11329 T14356 T14355 punct (,antibody
R11330 T14357 T14355 appos mAb72,antibody
R11331 T14358 T14355 punct ),antibody
R11332 T14359 T14316 punct .,incubated
R11333 T14360 T14363 nsubjpass Cells/beads,pelleted
R11334 T14361 T14363 auxpass were,pelleted
R11335 T14362 T14363 advmod then,pelleted
R11336 T14363 T14363 ROOT pelleted,pelleted
R11337 T14364 T14363 agent by,pelleted
R11338 T14365 T14364 pobj centrifugation,by
R11339 T14366 T14363 cc and,pelleted
R11340 T14367 T14363 conj lysed,pelleted
R11341 T14368 T14367 prep in,lysed
R11342 T14369 T14368 pobj buffer,in
R11343 T14370 T14369 acl containing,buffer
R11344 T14371 T14372 nummod 1,%
R11345 T14372 T14374 compound %,X100
R11346 T14373 T14374 compound Triton,X100
R11347 T14374 T14370 dobj X100,containing
R11348 T14375 T14363 punct .,pelleted
R11349 T14376 T14378 nsubjpass Beads,washed
R11350 T14377 T14378 auxpass were,washed
R11351 T14378 T14378 ROOT washed,washed
R11352 T14379 T14378 prep with,washed
R11353 T14380 T14381 compound lysis,buffer
R11354 T14381 T14379 pobj buffer,with
R11355 T14382 T14383 aux to,remove
R11356 T14383 T14378 advcl remove,washed
R11357 T14384 T14385 amod unbound,material
R11358 T14385 T14383 dobj material,remove
R11359 T14386 T14378 punct .,washed
R11360 T14387 T14397 prep In,dephosphorylated
R11361 T14388 T14389 det some,experiments
R11362 T14389 T14387 pobj experiments,In
R11363 T14390 T14397 punct ",",dephosphorylated
R11364 T14391 T14396 nsubj material,was
R11365 T14392 T14391 acl associated,material
R11366 T14393 T14392 prep with,associated
R11367 T14394 T14395 det the,beads
R11368 T14395 T14393 pobj beads,with
R11369 T14396 T14397 auxpass was,dephosphorylated
R11370 T14397 T14397 ROOT dephosphorylated,dephosphorylated
R11371 T14398 T14397 prep with,dephosphorylated
R11372 T14399 T14400 compound lambda,phosphatase
R11373 T14400 T14398 pobj phosphatase,with
R11374 T14401 T14404 punct (,BioLabs
R11375 T14402 T14404 compound New,BioLabs
R11376 T14403 T14404 compound England,BioLabs
R11377 T14404 T14400 appos BioLabs,phosphatase
R11378 T14405 T14404 punct ),BioLabs
R11379 T14406 T14397 prep as,dephosphorylated
R11380 T14407 T14406 prep per,as
R11381 T14408 T14410 poss manufacturer,instructions
R11382 T14409 T14408 case 's,manufacturer
R11383 T14410 T14407 pobj instructions,per
R11384 T14411 T14397 punct .,dephosphorylated
R11385 T14412 T14414 nsubjpass Beads,resuspended
R11386 T14413 T14414 auxpass were,resuspended
R11387 T14414 T14414 ROOT resuspended,resuspended
R11388 T14415 T14414 prep in,resuspended
R11389 T14416 T14417 compound 2X,SDS-PAGE
R11390 T14417 T14419 compound SDS-PAGE,buffer
R11391 T14418 T14419 compound sample,buffer
R11392 T14419 T14415 pobj buffer,in
R11393 T14420 T14414 cc and,resuspended
R11394 T14421 T14414 conj heated,resuspended
R11395 T14422 T14421 prep for,heated
R11396 T14423 T14424 nummod 5,minutes
R11397 T14424 T14422 pobj minutes,for
R11398 T14425 T14424 prep at,minutes
R11399 T14426 T14430 nummod 95,eluted
R11400 T14427 T14430 nummod °,eluted
R11401 T14428 T14429 compound C.,Material
R11402 T14429 T14430 compound Material,eluted
R11403 T14430 T14414 conj eluted,resuspended
R11404 T14431 T14430 prep from,eluted
R11405 T14432 T14433 det the,beads
R11406 T14433 T14431 pobj beads,from
R11407 T14434 T14435 auxpass was,fractionated
R11408 T14435 T14435 ROOT fractionated,fractionated
R11409 T14436 T14435 agent by,fractionated
R11410 T14437 T14436 pobj SDS-PAGE,by
R11411 T14438 T14435 cc and,fractionated
R11412 T14439 T14435 conj transferred,fractionated
R11413 T14440 T14439 prep to,transferred
R11414 T14441 T14442 compound PVDF,membranes
R11415 T14442 T14440 pobj membranes,to
R11416 T14443 T14442 prep for,membranes
R11417 T14444 T14445 amod Western,blotting
R11418 T14445 T14443 pobj blotting,for
R11419 T14446 T14435 punct .,fractionated
R11420 T14447 T14453 prep In,cultured
R11421 T14448 T14449 det some,experiments
R11422 T14449 T14447 pobj experiments,In
R11423 T14450 T14453 punct ",",cultured
R11424 T14451 T14453 nsubjpass cells,cultured
R11425 T14452 T14453 auxpass were,cultured
R11426 T14453 T14453 ROOT cultured,cultured
R11427 T14454 T14453 prep for,cultured
R11428 T14455 T14456 nummod 6,hrs
R11429 T14456 T14454 pobj hrs,for
R11430 T14457 T14456 prep with,hrs
R11431 T14458 T14459 nummod 25,µM
R11432 T14459 T14462 nmod µM,peptide
R11433 T14460 T14462 amod antennapedia-linked,peptide
R11434 T14461 T14462 compound SMAC-N7,peptide
R11435 T14462 T14457 pobj peptide,with
R11436 T14463 T14462 punct (,peptide
R11437 T14464 T14462 appos Calbiochem,peptide
R11438 T14465 T14462 punct ),peptide
R11439 T14466 T14462 cc or,peptide
R11440 T14467 T14469 det an,volume
R11441 T14468 T14469 amod appropriate,volume
R11442 T14469 T14453 dobj volume,cultured
R11443 T14470 T14469 prep of,volume
R11444 T14471 T14472 det the,solvent
R11445 T14472 T14470 pobj solvent,of
R11446 T14473 T14469 acl used,volume
R11447 T14474 T14473 prep for,used
R11448 T14475 T14476 det the,peptide
R11449 T14476 T14474 pobj peptide,for
R11450 T14477 T14476 punct (,peptide
R11451 T14478 T14476 appos DMSO,peptide
R11452 T14479 T14476 punct ),peptide
R11453 T14480 T14453 punct .,cultured
R11454 T14481 T14494 prep After,stimulated
R11455 T14482 T14481 pobj incubation,After
R11456 T14483 T14494 punct ",",stimulated
R11457 T14484 T14494 nsubjpass cells,stimulated
R11458 T14485 T14484 punct (,cells
R11459 T14486 T14484 prep in,cells
R11460 T14487 T14486 pobj peptide,in
R11461 T14488 T14486 punct -,in
R11462 T14489 T14486 cc or,in
R11463 T14490 T14491 amod DMSO-containing,medium
R11464 T14491 T14484 conj medium,cells
R11465 T14492 T14484 punct ),cells
R11466 T14493 T14494 auxpass were,stimulated
R11467 T14494 T14494 ROOT stimulated,stimulated
R11468 T14495 T14494 prep with,stimulated
R11469 T14496 T14497 amod antibody-coated,beads
R11470 T14497 T14495 pobj beads,with
R11471 T14498 T14499 mark as,outlined
R11472 T14499 T14494 advcl outlined,stimulated
R11473 T14500 T14499 prep above,outlined
R11474 T14501 T14494 punct .,stimulated

GO-BP

Id Subject Object Predicate Lexical cue
T14717 500-505 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T14718 630-641 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase

GO-MF

Id Subject Object Predicate Lexical cue
T14719 363-371 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T14720 1167-1175 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody
T14721 630-641 http://purl.obolibrary.org/obo/GO_0016791 denotes phosphatase

GO-CC

Id Subject Object Predicate Lexical cue
T14728 1167-1175 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody
T14729 870-879 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T14722 138-143 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14723 923-928 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14724 1100-1105 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14725 363-371 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T14726 1167-1175 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T14727 363-371 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody

sentences

Id Subject Object Predicate Lexical cue
T14275 0-20 Sentence denotes Immunoprecipitations
T14276 21-122 Sentence denotes Immunoprecipitation of CD40 was performed by activated receptor capture, as previously described [6].
T14277 123-380 Sentence denotes Briefly, 3×107 cells (A20.2J and derivatives) were incubated for 60 minutes at room temperature with 10 µl magnetic protein G beads (Dynal) pre-coated with 10 µg goat anti-rat IgG (Jackson), and 10 µg anti-CD40 (1C10) or an isotype control antibody (mAb72).
T14278 381-476 Sentence denotes Cells/beads were then pelleted by centrifugation and lysed in buffer containing 1% Triton X100.
T14279 477-540 Sentence denotes Beads were washed with lysis buffer to remove unbound material.
T14280 541-699 Sentence denotes In some experiments, material associated with the beads was dephosphorylated with lambda phosphatase (New England BioLabs) as per manufacturer's instructions.
T14281 700-901 Sentence denotes Beads were resuspended in 2X SDS-PAGE sample buffer and heated for 5 minutes at 95°C. Material eluted from the beads was fractionated by SDS-PAGE and transferred to PVDF membranes for Western blotting.
T14282 902-1081 Sentence denotes In some experiments, cells were cultured for 6 hrs with 25 µM antennapedia-linked SMAC-N7 peptide (Calbiochem) or an appropriate volume of the solvent used for the peptide (DMSO).
T14283 1082-1207 Sentence denotes After incubation, cells (in peptide- or DMSO-containing medium) were stimulated with antibody-coated beads as outlined above.
T231 0-20 Sentence denotes Immunoprecipitations
T232 21-122 Sentence denotes Immunoprecipitation of CD40 was performed by activated receptor capture, as previously described [6].
T233 123-380 Sentence denotes Briefly, 3×107 cells (A20.2J and derivatives) were incubated for 60 minutes at room temperature with 10 µl magnetic protein G beads (Dynal) pre-coated with 10 µg goat anti-rat IgG (Jackson), and 10 µg anti-CD40 (1C10) or an isotype control antibody (mAb72).
T234 381-476 Sentence denotes Cells/beads were then pelleted by centrifugation and lysed in buffer containing 1% Triton X100.
T235 477-540 Sentence denotes Beads were washed with lysis buffer to remove unbound material.
T236 541-699 Sentence denotes In some experiments, material associated with the beads was dephosphorylated with lambda phosphatase (New England BioLabs) as per manufacturer's instructions.
T237 700-785 Sentence denotes Beads were resuspended in 2X SDS-PAGE sample buffer and heated for 5 minutes at 95°C.
T238 786-901 Sentence denotes Material eluted from the beads was fractionated by SDS-PAGE and transferred to PVDF membranes for Western blotting.
T239 902-1081 Sentence denotes In some experiments, cells were cultured for 6 hrs with 25 µM antennapedia-linked SMAC-N7 peptide (Calbiochem) or an appropriate volume of the solvent used for the peptide (DMSO).
T240 1082-1207 Sentence denotes After incubation, cells (in peptide- or DMSO-containing medium) were stimulated with antibody-coated beads as outlined above.

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T14705 44-48 P25942 denotes CD40
T14706 145-148 P21580 denotes A20
T14707 329-333 P25942 denotes CD40

test2

Id Subject Object Predicate Lexical cue
T14273 44-48 Protein denotes CD40