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PMC:3148254 / 30093-31136 JSONTXT

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bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T12894 102-106 Protein denotes HOIP
T12895 111-115 Protein denotes IKKγ
T12896 306-310 Protein denotes HOIP
T12897 401-405 Protein denotes IKKγ
T12898 572-576 Protein denotes HOIP
T12899 586-590 Protein denotes IKKγ
T12900 719-723 Protein denotes HOIP
T12901 724-733 Negative_regulation denotes deficient
R10096 T12900 T12901 themeOf HOIP,deficient

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T13321 102-131 Protein denotes HOIP and IKKγ cDNA constructs
T13322 180-183 Protein denotes GFP
T13323 157-166 Negative_regulation denotes replacing
T13324 300-315 Protein denotes mouse HOIP cDNA
T13325 369-385 Protein denotes A pMIP construct
T13326 395-405 Protein denotes mouse IKKγ
T13327 457-460 Protein denotes tag
T13328 581-590 Protein denotes pMIP-IKKγ
R10475 T13322 T13323 themeOf GFP,replacing

events-check-again

Id Subject Object Predicate Lexical cue
T13329 102-106 Protein denotes HOIP
T13330 111-115 Protein denotes IKKγ
T13331 306-310 Protein denotes HOIP
T13332 401-405 Protein denotes IKKγ
T13333 572-576 Protein denotes HOIP
T13334 586-590 Protein denotes IKKγ
T13335 719-723 Protein denotes HOIP
T13336 724-733 Negative_regulation denotes deficient
R10476 T13335 T13336 themeOf HOIP,deficient

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T13106 0-10 JJ denotes Retroviral
T13107 11-23 NN denotes transduction
T13108 24-25 DT denotes A
T13109 26-36 JJ denotes retroviral
T13110 37-43 NN denotes vector
T13111 44-45 -LRB- denotes (
T13112 45-49 NN denotes pMIP
T13113 49-50 -RRB- denotes )
T13114 51-54 VB denotes was
T13115 55-59 VB denotes used
T13116 60-63 IN denotes for
T13117 64-67 DT denotes the
T13118 68-74 JJ denotes stable
T13119 75-87 NN denotes transduction
T13120 88-90 IN denotes of
T13121 91-96 NN denotes cells
T13122 97-101 IN denotes with
T13123 102-106 NN denotes HOIP
T13124 107-110 CC denotes and
T13125 111-115 NN denotes IKKγ
T13126 116-120 NN denotes cDNA
T13127 121-132 NN denotes constructs.
T13128 133-137 NN denotes pMIP
T13129 138-141 VB denotes was
T13130 142-153 VB denotes constructed
T13131 154-156 IN denotes by
T13132 157-166 VB denotes replacing
T13133 167-170 DT denotes the
T13134 171-175 NN denotes IRES
T13135 176-179 CC denotes and
T13136 180-183 NN denotes GFP
T13137 184-192 VB denotes encoding
T13138 193-202 NN denotes sequences
T13139 203-205 IN denotes in
T13140 206-210 NN denotes pMIG
T13141 211-212 -LRB- denotes [
T13142 212-214 CD denotes 31
T13143 214-215 -RRB- denotes ]
T13144 216-220 IN denotes with
T13145 221-224 DT denotes the
T13146 225-229 NN denotes IRES
T13147 230-233 CC denotes and
T13148 234-243 NN denotes puromycin
T13149 244-254 NN denotes resistance
T13150 255-259 NN denotes gene
T13151 260-264 IN denotes from
T13152 265-275 NN denotes pIRESpuro2
T13153 276-277 -LRB- denotes (
T13154 277-285 NN denotes Clontech
T13155 285-286 -RRB- denotes )
T13156 288-299 JJ denotes FLAG-tagged
T13157 300-305 NN denotes mouse
T13158 306-310 NN denotes HOIP
T13159 311-315 NN denotes cDNA
T13160 316-319 VB denotes was
T13161 320-328 VB denotes inserted
T13162 329-333 IN denotes into
T13163 334-337 DT denotes the
T13164 338-346 JJ denotes multiple
T13165 347-354 NN denotes cloning
T13166 355-359 NN denotes site
T13167 360-362 IN denotes of
T13168 363-367 NN denotes pMIP
T13169 369-370 DT denotes A
T13170 371-375 NN denotes pMIP
T13171 376-385 NN denotes construct
T13172 386-394 VB denotes encoding
T13173 395-400 NN denotes mouse
T13174 401-405 NN denotes IKKγ
T13175 406-410 IN denotes with
T13176 411-413 DT denotes an
T13177 414-419 JJ denotes amino
T13178 420-428 JJ denotes terminal
T13179 429-434 NN denotes birch
T13180 435-443 NN denotes profilin
T13181 444-451 NN denotes peptide
T13182 452-453 -LRB- denotes (
T13183 453-455 NN denotes BP
T13184 455-456 -RRB- denotes )
T13185 456-460 NN denotes -tag
T13186 461-464 VB denotes was
T13187 465-469 RB denotes also
T13188 470-478 VB denotes prepared
T13189 480-490 JJ denotes Retroviral
T13190 491-500 NN denotes particles
T13191 501-505 VB denotes were
T13192 506-515 VB denotes generated
T13193 516-518 IN denotes by
T13194 519-528 JJ denotes transient
T13195 529-541 NN denotes transfection
T13196 542-544 IN denotes of
T13197 545-549 NN denotes 293T
T13198 550-555 NN denotes cells
T13199 556-560 IN denotes with
T13200 561-565 NN denotes pMIP
T13201 565-566 -COMMA- denotes ,
T13202 567-576 NN denotes pMIP-HOIP
T13203 576-577 -COMMA- denotes ,
T13204 578-580 CC denotes or
T13205 581-590 NN denotes pMIP-IKKγ
T13206 591-594 CC denotes and
T13207 595-598 DT denotes the
T13208 599-608 NN denotes packaging
T13209 609-615 NN denotes vector
T13210 616-623 NN denotes pCL-Eco
T13211 624-625 -LRB- denotes [
T13212 625-627 CD denotes 32
T13213 627-628 -RRB- denotes ]
T13214 630-633 CD denotes Two
T13215 634-638 NN denotes days
T13216 639-644 IN denotes after
T13217 645-657 NN denotes transfection
T13218 657-658 -COMMA- denotes ,
T13219 659-666 NN denotes culture
T13220 667-679 NN denotes supernatants
T13221 680-684 VB denotes were
T13222 685-694 VB denotes harvested
T13223 695-698 CC denotes and
T13224 699-707 VB denotes filtered
T13225 709-715 NN denotes A20.2J
T13226 716-718 CC denotes or
T13227 719-733 JJ denotes HOIP-deficient
T13228 734-739 NN denotes cells
T13229 740-744 VB denotes were
T13230 745-750 VB denotes added
T13231 751-753 TO denotes to
T13232 754-761 JJ denotes 24-well
T13233 762-768 NN denotes plates
T13234 769-770 -LRB- denotes (
T13235 770-777 CD denotes 2.5×105
T13236 778-788 NN denotes cells/well
T13237 788-789 -RRB- denotes )
T13238 790-794 IN denotes with
T13239 795-796 CD denotes 1
T13240 797-799 NN denotes ml
T13241 800-816 JJ denotes virus-containing
T13242 817-828 NN denotes supernatant
T13243 829-832 CC denotes and
T13244 833-834 CD denotes 8
T13245 835-840 NN denotes µg/ml
T13246 841-850 NN denotes polybrene
T13247 852-858 NN denotes Plates
T13248 859-863 VB denotes were
T13249 864-875 VB denotes centrifuged
T13250 876-878 IN denotes at
T13251 879-882 CD denotes 500
T13252 883-884 SYM denotes x
T13253 885-886 NN denotes g
T13254 887-890 IN denotes for
T13255 891-892 CD denotes 2
T13256 893-896 NN denotes hrs
T13257 897-899 IN denotes at
T13258 900-904 NN denotes room
T13259 905-916 NN denotes temperature
T13260 918-923 NN denotes Cells
T13261 924-928 VB denotes were
T13262 929-937 VB denotes cultured
T13263 938-940 IN denotes in
T13264 941-942 NN denotes B
T13265 943-947 NN denotes cell
T13266 948-955 NN denotes culture
T13267 956-962 NN denotes medium
T13268 963-966 IN denotes for
T13269 967-969 CD denotes 48
T13270 970-973 NN denotes hrs
T13271 973-974 -COMMA- denotes ,
T13272 975-980 IN denotes after
T13273 981-986 WDT denotes which
T13274 987-990 DT denotes the
T13275 991-1001 VB denotes transduced
T13276 1002-1007 NN denotes cells
T13277 1008-1012 VB denotes were
T13278 1013-1021 VB denotes selected
T13279 1022-1024 IN denotes in
T13280 1025-1026 CD denotes 3
T13281 1027-1032 NN denotes µg/ml
T13282 1033-1042 NN denotes puromycin
R10292 T13107 T13106 arg1Of transduction,Retroviral
R10293 T13110 T13108 arg1Of vector,A
R10294 T13110 T13109 arg1Of vector,retroviral
R10295 T13110 T13111 arg1Of vector,(
R10296 T13110 T13114 arg1Of vector,was
R10297 T13110 T13115 arg2Of vector,used
R10298 T13112 T13111 arg2Of pMIP,(
R10299 T13113 T13111 arg3Of ),(
R10300 T13115 T13114 arg2Of used,was
R10301 T13115 T13116 arg1Of used,for
R10302 T13115 T13124 arg1Of used,and
R10303 T13119 T13116 arg2Of transduction,for
R10304 T13119 T13117 arg1Of transduction,the
R10305 T13119 T13118 arg1Of transduction,stable
R10306 T13119 T13120 arg1Of transduction,of
R10307 T13119 T13122 arg1Of transduction,with
R10308 T13121 T13120 arg2Of cells,of
R10309 T13123 T13122 arg2Of HOIP,with
R10310 T13128 T13125 arg1Of pMIP,IKKγ
R10311 T13128 T13126 arg1Of pMIP,cDNA
R10312 T13128 T13127 arg1Of pMIP,constructs.
R10313 T13128 T13129 arg1Of pMIP,was
R10314 T13128 T13130 arg2Of pMIP,constructed
R10315 T13130 T13124 arg2Of constructed,and
R10316 T13130 T13129 arg2Of constructed,was
R10317 T13130 T13131 arg1Of constructed,by
R10318 T13132 T13131 arg2Of replacing,by
R10319 T13132 T13141 arg1Of replacing,[
R10320 T13132 T13144 arg1Of replacing,with
R10321 T13134 T13135 arg1Of IRES,and
R10322 T13135 T13132 arg2Of and,replacing
R10323 T13135 T13133 arg1Of and,the
R10324 T13135 T13137 arg1Of and,encoding
R10325 T13136 T13135 arg2Of GFP,and
R10326 T13137 T13139 arg1Of encoding,in
R10327 T13138 T13137 arg2Of sequences,encoding
R10328 T13140 T13139 arg2Of pMIG,in
R10329 T13142 T13141 arg2Of 31,[
R10330 T13143 T13141 arg3Of ],[
R10331 T13146 T13147 arg1Of IRES,and
R10332 T13148 T13147 arg2Of puromycin,and
R10333 T13150 T13144 arg2Of gene,with
R10334 T13150 T13145 arg1Of gene,the
R10335 T13150 T13146 arg1Of gene,IRES
R10336 T13150 T13148 arg1Of gene,puromycin
R10337 T13150 T13149 arg1Of gene,resistance
R10338 T13150 T13151 arg1Of gene,from
R10339 T13152 T13151 arg2Of pIRESpuro2,from
R10340 T13152 T13153 arg1Of pIRESpuro2,(
R10341 T13154 T13153 arg2Of Clontech,(
R10342 T13155 T13153 arg3Of ),(
R10343 T13159 T13156 arg1Of cDNA,FLAG-tagged
R10344 T13159 T13157 arg1Of cDNA,mouse
R10345 T13159 T13158 arg1Of cDNA,HOIP
R10346 T13159 T13160 arg1Of cDNA,was
R10347 T13159 T13161 arg2Of cDNA,inserted
R10348 T13161 T13160 arg2Of inserted,was
R10349 T13161 T13162 arg1Of inserted,into
R10350 T13166 T13162 arg2Of site,into
R10351 T13166 T13163 arg1Of site,the
R10352 T13166 T13164 arg1Of site,multiple
R10353 T13166 T13165 arg1Of site,cloning
R10354 T13166 T13167 arg1Of site,of
R10355 T13168 T13167 arg2Of pMIP,of
R10356 T13171 T13169 arg1Of construct,A
R10357 T13171 T13170 arg1Of construct,pMIP
R10358 T13171 T13172 arg1Of construct,encoding
R10359 T13171 T13186 arg1Of construct,was
R10360 T13171 T13188 arg2Of construct,prepared
R10361 T13172 T13175 arg1Of encoding,with
R10362 T13174 T13172 arg2Of IKKγ,encoding
R10363 T13174 T13173 arg1Of IKKγ,mouse
R10364 T13181 T13177 arg1Of peptide,amino
R10365 T13181 T13178 arg1Of peptide,terminal
R10366 T13181 T13179 arg1Of peptide,birch
R10367 T13181 T13180 arg1Of peptide,profilin
R10368 T13181 T13182 arg1Of peptide,(
R10369 T13183 T13182 arg2Of BP,(
R10370 T13184 T13182 arg3Of ),(
R10371 T13185 T13175 arg2Of -tag,with
R10372 T13185 T13176 arg1Of -tag,an
R10373 T13185 T13181 arg1Of -tag,peptide
R10374 T13188 T13186 arg2Of prepared,was
R10375 T13188 T13187 arg1Of prepared,also
R10376 T13190 T13189 arg1Of particles,Retroviral
R10377 T13190 T13191 arg1Of particles,were
R10378 T13190 T13192 arg2Of particles,generated
R10379 T13192 T13191 arg2Of generated,were
R10380 T13192 T13193 arg1Of generated,by
R10381 T13195 T13193 arg2Of transfection,by
R10382 T13195 T13194 arg1Of transfection,transient
R10383 T13195 T13196 arg1Of transfection,of
R10384 T13195 T13199 arg1Of transfection,with
R10385 T13198 T13196 arg2Of cells,of
R10386 T13198 T13197 arg1Of cells,293T
R10387 T13200 T13201 arg1Of pMIP,","
R10388 T13201 T13204 arg1Of ",",or
R10389 T13202 T13201 arg2Of pMIP-HOIP,","
R10390 T13204 T13203 arg1Of or,","
R10391 T13204 T13206 arg1Of or,and
R10392 T13205 T13204 arg2Of pMIP-IKKγ,or
R10393 T13206 T13199 arg2Of and,with
R10394 T13210 T13206 arg2Of pCL-Eco,and
R10395 T13210 T13207 arg1Of pCL-Eco,the
R10396 T13210 T13208 arg1Of pCL-Eco,packaging
R10397 T13210 T13209 arg1Of pCL-Eco,vector
R10398 T13210 T13211 arg1Of pCL-Eco,[
R10399 T13212 T13211 arg2Of 32,[
R10400 T13213 T13211 arg3Of ],[
R10401 T13215 T13214 arg1Of days,Two
R10402 T13216 T13215 arg1Of after,days
R10403 T13217 T13216 arg2Of transfection,after
R10404 T13220 T13219 arg1Of supernatants,culture
R10405 T13220 T13221 arg1Of supernatants,were
R10406 T13220 T13222 arg2Of supernatants,harvested
R10407 T13220 T13224 arg2Of supernatants,filtered
R10408 T13222 T13223 arg1Of harvested,and
R10409 T13223 T13216 arg1Of and,after
R10410 T13223 T13218 arg1Of and,","
R10411 T13223 T13221 arg2Of and,were
R10412 T13224 T13223 arg2Of filtered,and
R10413 T13225 T13226 arg1Of A20.2J,or
R10414 T13227 T13226 arg2Of HOIP-deficient,or
R10415 T13228 T13225 arg1Of cells,A20.2J
R10416 T13228 T13227 arg1Of cells,HOIP-deficient
R10417 T13228 T13229 arg1Of cells,were
R10418 T13228 T13230 arg2Of cells,added
R10419 T13230 T13229 arg2Of added,were
R10420 T13230 T13231 arg1Of added,to
R10421 T13230 T13238 arg1Of added,with
R10422 T13233 T13231 arg2Of plates,to
R10423 T13233 T13232 arg1Of plates,24-well
R10424 T13233 T13234 arg1Of plates,(
R10425 T13236 T13234 arg2Of cells/well,(
R10426 T13236 T13235 arg1Of cells/well,2.5×105
R10427 T13237 T13234 arg3Of ),(
R10428 T13242 T13239 arg1Of supernatant,1
R10429 T13242 T13240 arg1Of supernatant,ml
R10430 T13242 T13241 arg1Of supernatant,virus-containing
R10431 T13242 T13243 arg1Of supernatant,and
R10432 T13243 T13238 arg2Of and,with
R10433 T13246 T13243 arg2Of polybrene,and
R10434 T13246 T13244 arg1Of polybrene,8
R10435 T13246 T13245 arg1Of polybrene,µg/ml
R10436 T13247 T13248 arg1Of Plates,were
R10437 T13247 T13249 arg2Of Plates,centrifuged
R10438 T13249 T13248 arg2Of centrifuged,were
R10439 T13249 T13250 arg1Of centrifuged,at
R10440 T13249 T13254 arg1Of centrifuged,for
R10441 T13253 T13250 arg2Of g,at
R10442 T13253 T13251 arg1Of g,500
R10443 T13253 T13252 arg1Of g,x
R10444 T13256 T13254 arg2Of hrs,for
R10445 T13256 T13255 arg1Of hrs,2
R10446 T13256 T13257 arg1Of hrs,at
R10447 T13259 T13257 arg2Of temperature,at
R10448 T13259 T13258 arg1Of temperature,room
R10449 T13260 T13261 arg1Of Cells,were
R10450 T13260 T13262 arg2Of Cells,cultured
R10451 T13262 T13261 arg2Of cultured,were
R10452 T13262 T13263 arg1Of cultured,in
R10453 T13262 T13268 arg1Of cultured,for
R10454 T13267 T13263 arg2Of medium,in
R10455 T13267 T13264 arg1Of medium,B
R10456 T13267 T13265 arg1Of medium,cell
R10457 T13267 T13266 arg1Of medium,culture
R10458 T13270 T13268 arg2Of hrs,for
R10459 T13270 T13269 arg1Of hrs,48
R10460 T13270 T13271 arg1Of hrs,","
R10461 T13270 T13272 arg2Of hrs,after
R10462 T13270 T13273 arg1Of hrs,which
R10463 T13276 T13274 arg1Of cells,the
R10464 T13276 T13275 arg2Of cells,transduced
R10465 T13276 T13277 arg1Of cells,were
R10466 T13276 T13278 arg2Of cells,selected
R10467 T13278 T13272 arg1Of selected,after
R10468 T13278 T13277 arg2Of selected,were
R10469 T13278 T13279 arg1Of selected,in
R10470 T13282 T13279 arg2Of puromycin,in
R10471 T13282 T13280 arg1Of puromycin,3
R10472 T13282 T13281 arg1Of puromycin,µg/ml

2_test

Id Subject Object Predicate Lexical cue
21829693-10514006-89653805 212-214 10514006 denotes 31
21829693-8764092-89653806 625-627 8764092 denotes 32
T34887 212-214 10514006 denotes 31
T49415 625-627 8764092 denotes 32

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T12911 0-10 NNP denotes Retroviral
T12912 11-23 NN denotes transduction
T12913 24-25 DT denotes A
T12914 26-36 JJ denotes retroviral
T12915 37-43 NN denotes vector
T12916 44-45 -LRB- denotes (
T12917 45-49 NNP denotes pMIP
T12918 49-50 -RRB- denotes )
T12919 51-54 VBD denotes was
T12920 55-59 VBN denotes used
T12921 60-63 IN denotes for
T12922 64-67 DT denotes the
T12923 68-74 JJ denotes stable
T12924 75-87 NN denotes transduction
T12925 88-90 IN denotes of
T12926 91-96 NNS denotes cells
T12927 97-101 IN denotes with
T12928 102-106 NNP denotes HOIP
T12929 107-110 CC denotes and
T12930 111-115 NNP denotes IKKγ
T12931 116-120 NNP denotes cDNA
T12932 121-131 NNS denotes constructs
T12933 131-132 . denotes .
T12934 133-137 NN denotes pMIP
T12935 138-141 VBD denotes was
T12936 142-153 VBN denotes constructed
T12937 154-156 IN denotes by
T12938 157-166 VBG denotes replacing
T12939 167-170 DT denotes the
T12940 171-175 NNP denotes IRES
T12941 176-179 CC denotes and
T12942 180-183 NNP denotes GFP
T12943 184-192 VBG denotes encoding
T12944 193-202 NNS denotes sequences
T12945 203-205 IN denotes in
T12946 206-210 NNP denotes pMIG
T12947 211-212 NNP denotes [
T12948 212-214 CD denotes 31
T12949 214-215 NN denotes ]
T12950 216-220 IN denotes with
T12951 221-224 DT denotes the
T12952 225-229 NNP denotes IRES
T12953 230-233 CC denotes and
T12954 234-243 JJ denotes puromycin
T12955 244-254 NN denotes resistance
T12956 255-259 NN denotes gene
T12957 260-264 IN denotes from
T12958 265-275 NNP denotes pIRESpuro2
T12959 276-277 -LRB- denotes (
T12960 277-285 NNP denotes Clontech
T12961 285-286 -RRB- denotes )
T12962 286-287 . denotes .
T12963 288-299 JJ denotes FLAG-tagged
T12964 300-305 NN denotes mouse
T12965 306-310 NNP denotes HOIP
T12966 311-315 NNP denotes cDNA
T12967 316-319 VBD denotes was
T12968 320-328 VBN denotes inserted
T12969 329-333 IN denotes into
T12970 334-337 DT denotes the
T12971 338-346 JJ denotes multiple
T12972 347-354 NN denotes cloning
T12973 355-359 NN denotes site
T12974 360-362 IN denotes of
T12975 363-367 NNP denotes pMIP
T12976 367-368 . denotes .
T12977 369-370 DT denotes A
T12978 371-375 NN denotes pMIP
T12979 376-385 VBP denotes construct
T12980 386-394 VBG denotes encoding
T12981 395-400 NN denotes mouse
T12982 401-405 NNP denotes IKKγ
T12983 406-410 IN denotes with
T12984 411-413 DT denotes an
T12985 414-419 JJ denotes amino
T12986 420-428 NN denotes terminal
T12987 429-434 NN denotes birch
T12988 435-443 NN denotes profilin
T12989 444-451 NN denotes peptide
T12990 452-453 -LRB- denotes (
T12991 453-455 NNP denotes BP
T12992 455-456 -RRB- denotes )
T12993 456-457 : denotes -
T12994 457-460 NN denotes tag
T12995 461-464 VBD denotes was
T12996 465-469 RB denotes also
T12997 470-478 VBN denotes prepared
T12998 478-479 . denotes .
T12999 480-490 NNP denotes Retroviral
T13000 491-500 NNS denotes particles
T13001 501-505 VBD denotes were
T13002 506-515 VBN denotes generated
T13003 516-518 IN denotes by
T13004 519-528 JJ denotes transient
T13005 529-541 NN denotes transfection
T13006 542-544 IN denotes of
T13007 545-549 JJ denotes 293T
T13008 550-555 NNS denotes cells
T13009 556-560 IN denotes with
T13010 561-565 NNP denotes pMIP
T13011 565-566 , denotes ,
T13012 567-576 NNP denotes pMIP-HOIP
T13013 576-577 , denotes ,
T13014 578-580 CC denotes or
T13015 581-590 NNP denotes pMIP-IKKγ
T13016 591-594 CC denotes and
T13017 595-598 DT denotes the
T13018 599-608 NN denotes packaging
T13019 609-615 NN denotes vector
T13020 616-623 NNP denotes pCL-Eco
T13021 624-625 NNP denotes [
T13022 625-627 CD denotes 32
T13023 627-628 NNP denotes ]
T13024 628-629 . denotes .
T13025 630-633 CD denotes Two
T13026 634-638 NNS denotes days
T13027 639-644 IN denotes after
T13028 645-657 NN denotes transfection
T13029 657-658 , denotes ,
T13030 659-666 NN denotes culture
T13031 667-679 NNS denotes supernatants
T13032 680-684 VBD denotes were
T13033 685-694 VBN denotes harvested
T13034 695-698 CC denotes and
T13035 699-707 VBN denotes filtered
T13036 707-708 . denotes .
T13037 709-712 CD denotes A20
T13038 712-714 CD denotes .2
T13039 714-715 NNP denotes J
T13040 716-718 CC denotes or
T13041 719-733 JJ denotes HOIP-deficient
T13042 734-739 NNS denotes cells
T13043 740-744 VBD denotes were
T13044 745-750 VBN denotes added
T13045 751-753 TO denotes to
T13046 754-761 JJ denotes 24-well
T13047 762-768 NNS denotes plates
T13048 769-770 -LRB- denotes (
T13049 770-773 CD denotes 2.5
T13050 773-774 CD denotes ×
T13051 774-777 CD denotes 105
T13052 778-788 NN denotes cells/well
T13053 788-789 -RRB- denotes )
T13054 790-794 IN denotes with
T13055 795-796 CD denotes 1
T13056 797-799 NN denotes ml
T13057 800-816 JJ denotes virus-containing
T13058 817-828 NN denotes supernatant
T13059 829-832 CC denotes and
T13060 833-834 CD denotes 8
T13061 835-837 NN denotes µg
T13062 837-838 NN denotes /
T13063 838-840 NN denotes ml
T13064 841-850 NN denotes polybrene
T13065 850-851 . denotes .
T13066 852-858 NNS denotes Plates
T13067 859-863 VBD denotes were
T13068 864-875 VBN denotes centrifuged
T13069 876-878 IN denotes at
T13070 879-882 CD denotes 500
T13071 883-884 SYM denotes x
T13072 885-886 FW denotes g
T13073 887-890 IN denotes for
T13074 891-892 CD denotes 2
T13075 893-896 NNS denotes hrs
T13076 897-899 IN denotes at
T13077 900-904 NN denotes room
T13078 905-916 NN denotes temperature
T13079 916-917 . denotes .
T13080 918-923 NNS denotes Cells
T13081 924-928 VBD denotes were
T13082 929-937 VBN denotes cultured
T13083 938-940 IN denotes in
T13084 941-942 NNP denotes B
T13085 943-947 NN denotes cell
T13086 948-955 NN denotes culture
T13087 956-962 NN denotes medium
T13088 963-966 IN denotes for
T13089 967-969 CD denotes 48
T13090 970-973 NNS denotes hrs
T13091 973-974 , denotes ,
T13092 975-980 IN denotes after
T13093 981-986 WDT denotes which
T13094 987-990 DT denotes the
T13095 991-1001 VBN denotes transduced
T13096 1002-1007 NNS denotes cells
T13097 1008-1012 VBD denotes were
T13098 1013-1021 VBN denotes selected
T13099 1022-1024 IN denotes in
T13100 1025-1026 CD denotes 3
T13101 1027-1029 NN denotes µg
T13102 1029-1030 NN denotes /
T13103 1030-1032 NN denotes ml
T13104 1033-1042 NN denotes puromycin
T13105 1042-1043 . denotes .
R10097 T12911 T12912 compound Retroviral,transduction
R10098 T12912 T12912 ROOT transduction,transduction
R10099 T12913 T12915 det A,vector
R10100 T12914 T12915 amod retroviral,vector
R10101 T12915 T12920 nsubjpass vector,used
R10102 T12916 T12917 punct (,pMIP
R10103 T12917 T12915 appos pMIP,vector
R10104 T12918 T12917 punct ),pMIP
R10105 T12919 T12920 auxpass was,used
R10106 T12920 T12920 ROOT used,used
R10107 T12921 T12920 prep for,used
R10108 T12922 T12924 det the,transduction
R10109 T12923 T12924 amod stable,transduction
R10110 T12924 T12921 pobj transduction,for
R10111 T12925 T12924 prep of,transduction
R10112 T12926 T12925 pobj cells,of
R10113 T12927 T12924 prep with,transduction
R10114 T12928 T12932 nmod HOIP,constructs
R10115 T12929 T12928 cc and,HOIP
R10116 T12930 T12931 compound IKKγ,cDNA
R10117 T12931 T12928 conj cDNA,HOIP
R10118 T12932 T12927 pobj constructs,with
R10119 T12933 T12920 punct .,used
R10120 T12934 T12936 nsubjpass pMIP,constructed
R10121 T12935 T12936 auxpass was,constructed
R10122 T12936 T12936 ROOT constructed,constructed
R10123 T12937 T12936 prep by,constructed
R10124 T12938 T12937 pcomp replacing,by
R10125 T12939 T12940 det the,IRES
R10126 T12940 T12938 dobj IRES,replacing
R10127 T12941 T12940 cc and,IRES
R10128 T12942 T12940 conj GFP,IRES
R10129 T12943 T12944 amod encoding,sequences
R10130 T12944 T12938 dobj sequences,replacing
R10131 T12945 T12938 prep in,replacing
R10132 T12946 T12947 compound pMIG,[
R10133 T12947 T12945 pobj [,in
R10134 T12948 T12949 nummod 31,]
R10135 T12949 T12947 appos ],[
R10136 T12950 T12949 prep with,]
R10137 T12951 T12952 det the,IRES
R10138 T12952 T12956 nmod IRES,gene
R10139 T12953 T12952 cc and,IRES
R10140 T12954 T12956 amod puromycin,gene
R10141 T12955 T12956 compound resistance,gene
R10142 T12956 T12950 pobj gene,with
R10143 T12957 T12956 prep from,gene
R10144 T12958 T12957 pobj pIRESpuro2,from
R10145 T12959 T12958 punct (,pIRESpuro2
R10146 T12960 T12958 appos Clontech,pIRESpuro2
R10147 T12961 T12958 punct ),pIRESpuro2
R10148 T12962 T12936 punct .,constructed
R10149 T12963 T12964 amod FLAG-tagged,mouse
R10150 T12964 T12966 compound mouse,cDNA
R10151 T12965 T12966 compound HOIP,cDNA
R10152 T12966 T12968 nsubjpass cDNA,inserted
R10153 T12967 T12968 auxpass was,inserted
R10154 T12968 T12968 ROOT inserted,inserted
R10155 T12969 T12968 prep into,inserted
R10156 T12970 T12973 det the,site
R10157 T12971 T12973 amod multiple,site
R10158 T12972 T12973 compound cloning,site
R10159 T12973 T12969 pobj site,into
R10160 T12974 T12973 prep of,site
R10161 T12975 T12974 pobj pMIP,of
R10162 T12976 T12968 punct .,inserted
R10163 T12977 T12978 det A,pMIP
R10164 T12978 T12979 nsubj pMIP,construct
R10165 T12979 T12979 ROOT construct,construct
R10166 T12980 T12981 amod encoding,mouse
R10167 T12981 T12982 compound mouse,IKKγ
R10168 T12982 T12979 dobj IKKγ,construct
R10169 T12983 T12979 prep with,construct
R10170 T12984 T12989 det an,peptide
R10171 T12985 T12987 amod amino,birch
R10172 T12986 T12987 compound terminal,birch
R10173 T12987 T12989 compound birch,peptide
R10174 T12988 T12989 compound profilin,peptide
R10175 T12989 T12983 pobj peptide,with
R10176 T12990 T12991 punct (,BP
R10177 T12991 T12989 appos BP,peptide
R10178 T12992 T12979 punct ),construct
R10179 T12993 T12994 punct -,tag
R10180 T12994 T12997 nsubjpass tag,prepared
R10181 T12995 T12997 auxpass was,prepared
R10182 T12996 T12997 advmod also,prepared
R10183 T12997 T12979 conj prepared,construct
R10184 T12998 T12997 punct .,prepared
R10185 T12999 T13000 compound Retroviral,particles
R10186 T13000 T13002 nsubjpass particles,generated
R10187 T13001 T13002 auxpass were,generated
R10188 T13002 T13002 ROOT generated,generated
R10189 T13003 T13002 agent by,generated
R10190 T13004 T13005 amod transient,transfection
R10191 T13005 T13003 pobj transfection,by
R10192 T13006 T13005 prep of,transfection
R10193 T13007 T13008 amod 293T,cells
R10194 T13008 T13006 pobj cells,of
R10195 T13009 T13005 prep with,transfection
R10196 T13010 T13009 pobj pMIP,with
R10197 T13011 T13010 punct ",",pMIP
R10198 T13012 T13010 conj pMIP-HOIP,pMIP
R10199 T13013 T13012 punct ",",pMIP-HOIP
R10200 T13014 T13012 cc or,pMIP-HOIP
R10201 T13015 T13012 conj pMIP-IKKγ,pMIP-HOIP
R10202 T13016 T13015 cc and,pMIP-IKKγ
R10203 T13017 T13019 det the,vector
R10204 T13018 T13019 compound packaging,vector
R10205 T13019 T13023 nmod vector,]
R10206 T13020 T13021 compound pCL-Eco,[
R10207 T13021 T13019 appos [,vector
R10208 T13022 T13023 nummod 32,]
R10209 T13023 T13015 conj ],pMIP-IKKγ
R10210 T13024 T13002 punct .,generated
R10211 T13025 T13026 nummod Two,days
R10212 T13026 T13027 npadvmod days,after
R10213 T13027 T13033 prep after,harvested
R10214 T13028 T13027 pobj transfection,after
R10215 T13029 T13033 punct ",",harvested
R10216 T13030 T13031 compound culture,supernatants
R10217 T13031 T13033 nsubjpass supernatants,harvested
R10218 T13032 T13033 auxpass were,harvested
R10219 T13033 T13033 ROOT harvested,harvested
R10220 T13034 T13033 cc and,harvested
R10221 T13035 T13033 conj filtered,harvested
R10222 T13036 T13033 punct .,harvested
R10223 T13037 T13039 nummod A20,J
R10224 T13038 T13039 nummod .2,J
R10225 T13039 T13042 nmod J,cells
R10226 T13040 T13039 cc or,J
R10227 T13041 T13039 conj HOIP-deficient,J
R10228 T13042 T13044 nsubjpass cells,added
R10229 T13043 T13044 auxpass were,added
R10230 T13044 T13044 ROOT added,added
R10231 T13045 T13044 prep to,added
R10232 T13046 T13047 amod 24-well,plates
R10233 T13047 T13045 pobj plates,to
R10234 T13048 T13047 punct (,plates
R10235 T13049 T13052 nummod 2.5,cells/well
R10236 T13050 T13052 nummod ×,cells/well
R10237 T13051 T13052 nummod 105,cells/well
R10238 T13052 T13047 appos cells/well,plates
R10239 T13053 T13047 punct ),plates
R10240 T13054 T13044 prep with,added
R10241 T13055 T13056 nummod 1,ml
R10242 T13056 T13058 nmod ml,supernatant
R10243 T13057 T13058 amod virus-containing,supernatant
R10244 T13058 T13054 pobj supernatant,with
R10245 T13059 T13058 cc and,supernatant
R10246 T13060 T13061 nummod 8,µg
R10247 T13061 T13064 nmod µg,polybrene
R10248 T13062 T13064 nmod /,polybrene
R10249 T13063 T13064 compound ml,polybrene
R10250 T13064 T13058 conj polybrene,supernatant
R10251 T13065 T13044 punct .,added
R10252 T13066 T13068 nsubjpass Plates,centrifuged
R10253 T13067 T13068 auxpass were,centrifuged
R10254 T13068 T13068 ROOT centrifuged,centrifuged
R10255 T13069 T13068 prep at,centrifuged
R10256 T13070 T13072 nummod 500,g
R10257 T13071 T13072 punct x,g
R10258 T13072 T13069 pobj g,at
R10259 T13073 T13072 prep for,g
R10260 T13074 T13075 nummod 2,hrs
R10261 T13075 T13073 pobj hrs,for
R10262 T13076 T13072 prep at,g
R10263 T13077 T13078 compound room,temperature
R10264 T13078 T13076 pobj temperature,at
R10265 T13079 T13068 punct .,centrifuged
R10266 T13080 T13082 nsubjpass Cells,cultured
R10267 T13081 T13082 auxpass were,cultured
R10268 T13082 T13082 ROOT cultured,cultured
R10269 T13083 T13082 prep in,cultured
R10270 T13084 T13086 compound B,culture
R10271 T13085 T13086 compound cell,culture
R10272 T13086 T13087 compound culture,medium
R10273 T13087 T13083 pobj medium,in
R10274 T13088 T13082 prep for,cultured
R10275 T13089 T13090 nummod 48,hrs
R10276 T13090 T13088 pobj hrs,for
R10277 T13091 T13082 punct ",",cultured
R10278 T13092 T13098 prep after,selected
R10279 T13093 T13092 pobj which,after
R10280 T13094 T13096 det the,cells
R10281 T13095 T13096 amod transduced,cells
R10282 T13096 T13098 nsubjpass cells,selected
R10283 T13097 T13098 auxpass were,selected
R10284 T13098 T13082 advcl selected,cultured
R10285 T13099 T13098 prep in,selected
R10286 T13100 T13101 nummod 3,µg
R10287 T13101 T13104 nmod µg,puromycin
R10288 T13102 T13104 nmod /,puromycin
R10289 T13103 T13104 compound ml,puromycin
R10290 T13104 T13099 pobj puromycin,in
R10291 T13105 T13082 punct .,cultured

GO-BP

Id Subject Object Predicate Lexical cue
T13298 11-23 http://purl.obolibrary.org/obo/GO_0009293 denotes transduction
T13299 75-87 http://purl.obolibrary.org/obo/GO_0009293 denotes transduction

GO-CC

Id Subject Object Predicate Lexical cue
T13300 91-96 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13301 550-555 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13302 734-739 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13303 778-783 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T13304 1002-1007 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

sentences

Id Subject Object Predicate Lexical cue
T12902 0-23 Sentence denotes Retroviral transduction
T12903 24-287 Sentence denotes A retroviral vector (pMIP) was used for the stable transduction of cells with HOIP and IKKγ cDNA constructs. pMIP was constructed by replacing the IRES and GFP encoding sequences in pMIG [31] with the IRES and puromycin resistance gene from pIRESpuro2 (Clontech).
T12904 288-368 Sentence denotes FLAG-tagged mouse HOIP cDNA was inserted into the multiple cloning site of pMIP.
T12905 369-479 Sentence denotes A pMIP construct encoding mouse IKKγ with an amino terminal birch profilin peptide (BP)-tag was also prepared.
T12906 480-629 Sentence denotes Retroviral particles were generated by transient transfection of 293T cells with pMIP, pMIP-HOIP, or pMIP-IKKγ and the packaging vector pCL-Eco [32].
T12907 630-708 Sentence denotes Two days after transfection, culture supernatants were harvested and filtered.
T12908 709-851 Sentence denotes A20.2J or HOIP-deficient cells were added to 24-well plates (2.5×105 cells/well) with 1 ml virus-containing supernatant and 8 µg/ml polybrene.
T12909 852-917 Sentence denotes Plates were centrifuged at 500 x g for 2 hrs at room temperature.
T12910 918-1043 Sentence denotes Cells were cultured in B cell culture medium for 48 hrs, after which the transduced cells were selected in 3 µg/ml puromycin.
T203 0-23 Sentence denotes Retroviral transduction
T204 24-287 Sentence denotes A retroviral vector (pMIP) was used for the stable transduction of cells with HOIP and IKKγ cDNA constructs. pMIP was constructed by replacing the IRES and GFP encoding sequences in pMIG [31] with the IRES and puromycin resistance gene from pIRESpuro2 (Clontech).
T205 288-368 Sentence denotes FLAG-tagged mouse HOIP cDNA was inserted into the multiple cloning site of pMIP.
T206 369-479 Sentence denotes A pMIP construct encoding mouse IKKγ with an amino terminal birch profilin peptide (BP)-tag was also prepared.
T207 480-629 Sentence denotes Retroviral particles were generated by transient transfection of 293T cells with pMIP, pMIP-HOIP, or pMIP-IKKγ and the packaging vector pCL-Eco [32].
T208 630-708 Sentence denotes Two days after transfection, culture supernatants were harvested and filtered.
T209 709-851 Sentence denotes A20.2J or HOIP-deficient cells were added to 24-well plates (2.5×105 cells/well) with 1 ml virus-containing supernatant and 8 µg/ml polybrene.
T210 852-917 Sentence denotes Plates were centrifuged at 500 x g for 2 hrs at room temperature.
T211 918-1043 Sentence denotes Cells were cultured in B cell culture medium for 48 hrs, after which the transduced cells were selected in 3 µg/ml puromycin.

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T13283 102-106 Q96EP0 denotes HOIP
T13284 180-183 Q9U6Y4 denotes GFP
T13285 306-310 Q96EP0 denotes HOIP
T13286 572-576 Q96EP0 denotes HOIP
T13287 709-712 P21580 denotes A20
T13288 719-723 Q96EP0 denotes HOIP

test2

Id Subject Object Predicate Lexical cue
T12886 102-106 Protein denotes HOIP
T12887 111-115 Protein denotes IKKγ
T12888 306-310 Protein denotes HOIP
T12889 401-405 Protein denotes IKKγ
T12890 572-576 Protein denotes HOIP
T12891 586-590 Protein denotes IKKγ
T12892 719-723 Protein denotes HOIP
T12893 724-733 Negative_regulation denotes deficient
R10095 T12892 T12893 themeOf HOIP,deficient