> top > docs > PMC:3148254 > spans > 28570-30091 > annotations

PMC:3148254 / 28570-30091 JSONTXT

Annnotations TAB JSON ListView MergeView

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue Speculation
T12312 46-50 Protein denotes HOIP
T12313 51-60 Negative_regulation denotes deficient
T12314 169-174 Protein denotes Rnf31
T12315 1176-1181 Protein denotes Rnf31
T12316 1191-1200 Negative_regulation denotes disrupted
T12317 1400-1405 Protein denotes Rnf31
T12318 1481-1485 Protein denotes HOIP
T12319 1494-1504 Gene_expression denotes expression true
R9582 T12312 T12313 themeOf HOIP,deficient
R9583 T12315 T12316 themeOf Rnf31,disrupted
R9584 T12318 T12319 themeOf HOIP,expression

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T12859 169-174 Protein denotes Rnf31
T12860 1141-1145 Protein denotes NeoR
T12861 1176-1181 Protein denotes Rnf31
T12862 1266-1269 Protein denotes Cre
T12863 1290-1294 Protein denotes NeoR
T12864 1238-1248 Gene_expression denotes expression
T12865 1400-1405 Protein denotes Rnf31
T12866 1473-1476 Protein denotes PCR
T12867 1481-1493 Protein denotes HOIP protein
T12868 1494-1504 Gene_expression denotes expression
T12869 1494-1504 Gene_expression denotes expression
R10088 T12862 T12864 themeOf Cre,expression
R10089 T12866 T12868 themeOf PCR,expression
R10090 T12867 T12869 themeOf HOIP protein,expression

events-check-again

Id Subject Object Predicate Lexical cue Speculation
T12870 46-50 Protein denotes HOIP
T12871 51-60 Negative_regulation denotes deficient
T12872 169-174 Protein denotes Rnf31
T12873 1176-1181 Protein denotes Rnf31
T12874 1191-1200 Negative_regulation denotes disrupted
T12875 1400-1405 Protein denotes Rnf31
T12876 1481-1485 Protein denotes HOIP
T12877 1494-1504 Gene_expression denotes expression true
R10091 T12870 T12871 themeOf HOIP,deficient
R10092 T12873 T12874 themeOf Rnf31,disrupted
R10093 T12876 T12877 themeOf HOIP,expression

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T12597 0-7 JJ denotes Somatic
T12598 8-12 NN denotes cell
T12599 13-17 NN denotes gene
T12600 18-27 VB denotes targeting
T12601 28-31 DT denotes The
T12602 32-42 NN denotes generation
T12603 43-45 IN denotes of
T12604 46-60 JJ denotes HOIP-deficient
T12605 61-66 NN denotes cells
T12606 67-70 VB denotes was
T12607 71-83 VB denotes accomplished
T12608 84-89 VB denotes using
T12609 90-91 DT denotes a
T12610 92-102 JJ denotes homologous
T12611 103-116 NN denotes recombination
T12612 117-125 NN denotes approach
T12613 126-135 VB denotes described
T12614 136-146 RB denotes previously
T12615 147-148 -LRB- denotes [
T12616 148-149 CD denotes 7
T12617 149-150 -RRB- denotes ]
T12618 150-151 -COMMA- denotes ,
T12619 152-153 -LRB- denotes [
T12620 153-154 CD denotes 8
T12621 154-155 -RRB- denotes ]
T12622 157-165 NN denotes Segments
T12623 166-168 IN denotes of
T12624 169-174 NN denotes Rnf31
T12625 175-179 NN denotes gene
T12626 180-188 NN denotes sequence
T12627 189-193 VB denotes used
T12628 194-196 IN denotes in
T12629 197-200 DT denotes the
T12630 201-210 VB denotes targeting
T12631 211-220 NN denotes construct
T12632 221-222 -LRB- denotes (
T12633 222-226 NN denotes Fig.
T12634 227-229 NN denotes 1A
T12635 229-230 -RRB- denotes )
T12636 231-235 VB denotes were
T12637 236-245 VB denotes amplified
T12638 246-248 IN denotes by
T12639 249-252 NN denotes PCR
T12640 253-257 IN denotes from
T12641 258-264 NN denotes A20.2J
T12642 265-272 JJ denotes genomic
T12643 273-276 NN denotes DNA
T12644 278-281 DT denotes The
T12645 282-297 NN denotes oligonucleotide
T12646 298-305 NN denotes primers
T12647 306-310 VB denotes used
T12648 311-313 TO denotes to
T12649 314-322 VB denotes generate
T12650 323-326 DT denotes the
T12651 327-329 NN denotes 5′
T12652 330-335 NN denotes flank
T12653 336-337 -LRB- denotes (
T12654 337-341 CD denotes 1224
T12655 342-344 NN denotes bp
T12656 344-345 -RRB- denotes )
T12657 346-350 VB denotes were
T12658 351-385 JJ denotes 5′-ttttctagagcggtggcttaagtgaccc-3′
T12659 386-389 CC denotes and
T12660 390-427 JJ denotes 5′-tattctagatgcagcatctgagaaagcaagc-3′
T12661 429-432 DT denotes The
T12662 433-435 NN denotes 3′
T12663 436-441 NN denotes flank
T12664 442-443 -LRB- denotes (
T12665 443-447 CD denotes 6032
T12666 448-450 NN denotes bp
T12667 450-451 -RRB- denotes )
T12668 452-459 NN denotes primers
T12669 460-464 VB denotes were
T12670 465-510 JJ denotes 5′-aaaaccggtgtatgcttctttacgggagaaaaatattag-3′
T12671 511-514 CC denotes and
T12672 515-554 JJ denotes 5′-tataccggtatgaagccaaaggaacactgagag-3′
T12673 556-567 NN denotes Restriction
T12674 568-580 NN denotes endonuclease
T12675 581-586 NN denotes sites
T12676 587-589 IN denotes in
T12677 590-593 DT denotes the
T12678 594-609 NN denotes oligonucleotide
T12679 610-617 NN denotes primers
T12680 618-625 VB denotes allowed
T12681 626-635 NN denotes insertion
T12682 636-638 IN denotes of
T12683 639-642 DT denotes the
T12684 643-646 NN denotes PCR
T12685 647-655 NN denotes products
T12686 656-660 IN denotes into
T12687 661-664 DT denotes the
T12688 665-674 NN denotes targeting
T12689 675-681 NN denotes vector
T12690 683-689 NN denotes A20.2J
T12691 690-695 NN denotes cells
T12692 696-700 VB denotes were
T12693 701-712 VB denotes transfected
T12694 713-714 -LRB- denotes (
T12695 714-716 IN denotes by
T12696 717-732 NN denotes electroporation
T12697 733-734 -LRB- denotes [
T12698 734-735 CD denotes 7
T12699 735-736 -RRB- denotes ]
T12700 736-737 -RRB- denotes )
T12701 738-742 IN denotes with
T12702 743-746 DT denotes the
T12703 747-756 VB denotes targeting
T12704 757-766 NN denotes construct
T12705 767-770 CC denotes and
T12706 771-780 VB denotes subcloned
T12707 781-783 IN denotes in
T12708 784-790 NN denotes medium
T12709 791-801 VB denotes containing
T12710 802-805 CD denotes 600
T12711 806-811 NN denotes µg/ml
T12712 812-816 NN denotes G418
T12713 817-824 NN denotes sulfate
T12714 826-836 JJ denotes Homologous
T12715 837-850 NN denotes recombination
T12716 851-853 IN denotes in
T12717 854-868 JJ denotes G418-resistant
T12718 869-875 NN denotes clones
T12719 876-879 VB denotes was
T12720 880-888 VB denotes detected
T12721 889-891 IN denotes by
T12722 892-895 NN denotes PCR
T12723 896-898 IN denotes of
T12724 899-906 JJ denotes genomic
T12725 907-910 NN denotes DNA
T12726 910-911 -COMMA- denotes ,
T12727 912-914 IN denotes as
T12728 915-924 VB denotes described
T12729 925-926 -LRB- denotes [
T12730 926-927 CD denotes 7
T12731 927-928 -RRB- denotes ]
T12732 930-945 NN denotes Oligonucleotide
T12733 946-953 NN denotes primers
T12734 954-958 VB denotes used
T12735 959-962 IN denotes for
T12736 963-972 NN denotes screening
T12737 973-977 VB denotes were
T12738 978-1010 JJ denotes 5′-cttcctgatctcagctttaccgtcac-3′
T12739 1011-1012 -LRB- denotes (
T12740 1012-1022 JJ denotes homologous
T12741 1023-1025 TO denotes to
T12742 1026-1033 JJ denotes genomic
T12743 1034-1042 NN denotes sequence
T12744 1042-1043 -COLON- denotes ;
T12745 1044-1055 JJ denotes approximate
T12746 1056-1064 NN denotes position
T12747 1065-1070 VB denotes noted
T12748 1071-1073 IN denotes in
T12749 1074-1078 NNP denotes Fig.
T12750 1079-1080 CD denotes 1
T12751 1080-1081 -RRB- denotes )
T12752 1082-1085 CC denotes and
T12753 1086-1113 JJ denotes 5′-caatccatcttgttcagccat-3′
T12754 1114-1115 -LRB- denotes (
T12755 1115-1125 JJ denotes homologous
T12756 1126-1128 TO denotes to
T12757 1129-1137 NN denotes sequence
T12758 1138-1140 IN denotes in
T12759 1141-1145 NN denotes NeoR
T12760 1145-1146 -RRB- denotes )
T12761 1148-1154 NN denotes Clones
T12762 1155-1157 IN denotes in
T12763 1158-1163 WDT denotes which
T12764 1164-1167 CD denotes one
T12765 1168-1172 NN denotes copy
T12766 1173-1175 IN denotes of
T12767 1176-1181 NN denotes Rnf31
T12768 1182-1185 VB denotes had
T12769 1186-1190 VB denotes been
T12770 1191-1200 VB denotes disrupted
T12771 1201-1205 VB denotes were
T12772 1206-1217 RB denotes transiently
T12773 1218-1229 VB denotes transfected
T12774 1230-1234 IN denotes with
T12775 1235-1237 DT denotes an
T12776 1238-1248 NN denotes expression
T12777 1249-1256 NN denotes plasmid
T12778 1257-1265 VB denotes encoding
T12779 1266-1269 NNP denotes Cre
T12780 1269-1270 -COMMA- denotes ,
T12781 1271-1273 IN denotes in
T12782 1274-1279 NN denotes order
T12783 1280-1282 TO denotes to
T12784 1283-1289 VB denotes remove
T12785 1290-1294 NN denotes NeoR
T12786 1296-1310 JJ denotes G418-sensitive
T12787 1311-1320 NN denotes subclones
T12788 1321-1325 VB denotes were
T12789 1326-1335 VB denotes subjected
T12790 1336-1338 TO denotes to
T12791 1339-1340 DT denotes a
T12792 1341-1347 JJ denotes second
T12793 1348-1353 NN denotes round
T12794 1354-1356 IN denotes of
T12795 1357-1366 VB denotes targeting
T12796 1367-1369 TO denotes to
T12797 1370-1377 VB denotes disrupt
T12798 1378-1381 DT denotes the
T12799 1382-1391 VB denotes remaining
T12800 1392-1396 NN denotes copy
T12801 1397-1399 IN denotes of
T12802 1400-1405 NN denotes Rnf31
T12803 1407-1421 JJ denotes G418-resistant
T12804 1422-1428 NN denotes clones
T12805 1429-1433 VB denotes were
T12806 1434-1440 VB denotes tested
T12807 1441-1444 IN denotes for
T12808 1445-1455 JJ denotes homologous
T12809 1456-1469 NN denotes recombination
T12810 1470-1472 IN denotes by
T12811 1473-1476 NN denotes PCR
T12812 1477-1480 CC denotes and
T12813 1481-1485 NN denotes HOIP
T12814 1486-1493 NN denotes protein
T12815 1494-1504 NN denotes expression
T12816 1505-1507 IN denotes by
T12817 1508-1515 JJ denotes Western
T12818 1516-1520 NN denotes blot
R9850 T12599 T12597 arg1Of gene,Somatic
R9851 T12599 T12598 arg1Of gene,cell
R9852 T12600 T12599 arg1Of targeting,gene
R9853 T12602 T12601 arg1Of generation,The
R9854 T12602 T12603 arg1Of generation,of
R9855 T12602 T12606 arg1Of generation,was
R9856 T12602 T12607 arg2Of generation,accomplished
R9857 T12605 T12603 arg2Of cells,of
R9858 T12605 T12604 arg1Of cells,HOIP-deficient
R9859 T12607 T12606 arg2Of accomplished,was
R9860 T12607 T12608 modOf accomplished,using
R9861 T12607 T12618 arg1Of accomplished,","
R9862 T12607 T12619 arg1Of accomplished,[
R9863 T12612 T12608 arg2Of approach,using
R9864 T12612 T12609 arg1Of approach,a
R9865 T12612 T12610 arg1Of approach,homologous
R9866 T12612 T12611 arg1Of approach,recombination
R9867 T12612 T12613 arg2Of approach,described
R9868 T12613 T12614 arg1Of described,previously
R9869 T12613 T12615 arg1Of described,[
R9870 T12616 T12615 arg2Of 7,[
R9871 T12617 T12615 arg3Of ],[
R9872 T12620 T12619 arg2Of 8,[
R9873 T12621 T12619 arg3Of ],[
R9874 T12622 T12623 arg1Of Segments,of
R9875 T12622 T12636 arg1Of Segments,were
R9876 T12622 T12637 arg2Of Segments,amplified
R9877 T12626 T12623 arg2Of sequence,of
R9878 T12626 T12624 arg1Of sequence,Rnf31
R9879 T12626 T12625 arg1Of sequence,gene
R9880 T12626 T12627 arg2Of sequence,used
R9881 T12627 T12628 arg1Of used,in
R9882 T12629 T12630 arg1Of the,targeting
R9883 T12630 T12628 arg2Of targeting,in
R9884 T12630 T12629 arg1Of targeting,the
R9885 T12631 T12630 arg2Of construct,targeting
R9886 T12631 T12632 arg1Of construct,(
R9887 T12634 T12632 arg2Of 1A,(
R9888 T12634 T12633 arg1Of 1A,Fig.
R9889 T12635 T12632 arg3Of ),(
R9890 T12637 T12636 arg2Of amplified,were
R9891 T12639 T12637 arg1Of PCR,amplified
R9892 T12639 T12638 arg2Of PCR,by
R9893 T12639 T12640 arg1Of PCR,from
R9894 T12643 T12640 arg2Of DNA,from
R9895 T12643 T12641 arg1Of DNA,A20.2J
R9896 T12643 T12642 arg1Of DNA,genomic
R9897 T12646 T12644 arg1Of primers,The
R9898 T12646 T12645 arg1Of primers,oligonucleotide
R9899 T12646 T12647 arg2Of primers,used
R9900 T12646 T12649 arg1Of primers,generate
R9901 T12646 T12657 arg1Of primers,were
R9902 T12646 T12658 arg1Of primers,5′-ttttctagagcggtggcttaagtgaccc-3′
R9903 T12646 T12660 arg1Of primers,5′-tattctagatgcagcatctgagaaagcaagc-3′
R9904 T12649 T12647 arg3Of generate,used
R9905 T12649 T12648 arg1Of generate,to
R9906 T12652 T12649 arg2Of flank,generate
R9907 T12652 T12650 arg1Of flank,the
R9908 T12652 T12651 arg1Of flank,5′
R9909 T12652 T12653 arg1Of flank,(
R9910 T12655 T12653 arg2Of bp,(
R9911 T12655 T12654 arg1Of bp,1224
R9912 T12656 T12653 arg3Of ),(
R9913 T12658 T12659 arg1Of 5′-ttttctagagcggtggcttaagtgaccc-3′,and
R9914 T12659 T12657 arg2Of and,were
R9915 T12660 T12659 arg2Of 5′-tattctagatgcagcatctgagaaagcaagc-3′,and
R9916 T12663 T12661 arg1Of flank,The
R9917 T12663 T12662 arg1Of flank,3′
R9918 T12663 T12664 arg1Of flank,(
R9919 T12666 T12664 arg2Of bp,(
R9920 T12666 T12665 arg1Of bp,6032
R9921 T12667 T12664 arg3Of ),(
R9922 T12668 T12663 arg1Of primers,flank
R9923 T12668 T12669 arg1Of primers,were
R9924 T12668 T12670 arg1Of primers,5′-aaaaccggtgtatgcttctttacgggagaaaaatattag-3′
R9925 T12668 T12672 arg1Of primers,5′-tataccggtatgaagccaaaggaacactgagag-3′
R9926 T12670 T12671 arg1Of 5′-aaaaccggtgtatgcttctttacgggagaaaaatattag-3′,and
R9927 T12671 T12669 arg2Of and,were
R9928 T12672 T12671 arg2Of 5′-tataccggtatgaagccaaaggaacactgagag-3′,and
R9929 T12675 T12673 arg1Of sites,Restriction
R9930 T12675 T12674 arg1Of sites,endonuclease
R9931 T12675 T12676 arg1Of sites,in
R9932 T12675 T12680 arg1Of sites,allowed
R9933 T12679 T12676 arg2Of primers,in
R9934 T12679 T12677 arg1Of primers,the
R9935 T12679 T12678 arg1Of primers,oligonucleotide
R9936 T12681 T12680 arg2Of insertion,allowed
R9937 T12681 T12682 arg1Of insertion,of
R9938 T12685 T12682 arg2Of products,of
R9939 T12685 T12683 arg1Of products,the
R9940 T12685 T12684 arg1Of products,PCR
R9941 T12685 T12686 arg1Of products,into
R9942 T12689 T12686 arg2Of vector,into
R9943 T12689 T12687 arg1Of vector,the
R9944 T12689 T12688 arg1Of vector,targeting
R9945 T12691 T12690 arg1Of cells,A20.2J
R9946 T12691 T12692 arg1Of cells,were
R9947 T12691 T12693 arg2Of cells,transfected
R9948 T12691 T12706 arg2Of cells,subcloned
R9949 T12693 T12694 arg1Of transfected,(
R9950 T12693 T12701 arg1Of transfected,with
R9951 T12693 T12705 arg1Of transfected,and
R9952 T12695 T12694 arg2Of by,(
R9953 T12696 T12695 arg2Of electroporation,by
R9954 T12696 T12697 arg1Of electroporation,[
R9955 T12698 T12697 arg2Of 7,[
R9956 T12699 T12697 arg3Of ],[
R9957 T12700 T12694 arg3Of ),(
R9958 T12702 T12703 arg1Of the,targeting
R9959 T12703 T12701 arg2Of targeting,with
R9960 T12703 T12702 arg1Of targeting,the
R9961 T12704 T12703 arg2Of construct,targeting
R9962 T12705 T12692 arg2Of and,were
R9963 T12706 T12705 arg2Of subcloned,and
R9964 T12706 T12707 arg1Of subcloned,in
R9965 T12708 T12707 arg2Of medium,in
R9966 T12708 T12709 arg1Of medium,containing
R9967 T12713 T12709 arg2Of sulfate,containing
R9968 T12713 T12710 arg1Of sulfate,600
R9969 T12713 T12711 arg1Of sulfate,µg/ml
R9970 T12713 T12712 arg1Of sulfate,G418
R9971 T12715 T12714 arg1Of recombination,Homologous
R9972 T12715 T12716 arg1Of recombination,in
R9973 T12715 T12719 arg1Of recombination,was
R9974 T12715 T12720 arg2Of recombination,detected
R9975 T12718 T12716 arg2Of clones,in
R9976 T12718 T12717 arg1Of clones,G418-resistant
R9977 T12720 T12719 arg2Of detected,was
R9978 T12720 T12726 arg1Of detected,","
R9979 T12720 T12727 arg1Of detected,as
R9980 T12722 T12720 arg1Of PCR,detected
R9981 T12722 T12721 arg2Of PCR,by
R9982 T12722 T12723 arg1Of PCR,of
R9983 T12725 T12723 arg2Of DNA,of
R9984 T12725 T12724 arg1Of DNA,genomic
R9985 T12728 T12727 arg2Of described,as
R9986 T12728 T12729 arg1Of described,[
R9987 T12730 T12729 arg2Of 7,[
R9988 T12731 T12729 arg3Of ],[
R9989 T12733 T12732 arg1Of primers,Oligonucleotide
R9990 T12733 T12734 arg2Of primers,used
R9991 T12733 T12737 arg1Of primers,were
R9992 T12733 T12738 arg1Of primers,5′-cttcctgatctcagctttaccgtcac-3′
R9993 T12733 T12753 arg1Of primers,5′-caatccatcttgttcagccat-3′
R9994 T12734 T12735 arg1Of used,for
R9995 T12736 T12735 arg2Of screening,for
R9996 T12738 T12739 arg1Of 5′-cttcctgatctcagctttaccgtcac-3′,(
R9997 T12738 T12752 arg1Of 5′-cttcctgatctcagctttaccgtcac-3′,and
R9998 T12740 T12741 arg1Of homologous,to
R9999 T12740 T12744 arg1Of homologous,;
R10000 T12743 T12741 arg2Of sequence,to
R10001 T12743 T12742 arg1Of sequence,genomic
R10002 T12745 T12744 arg2Of approximate,;
R10003 T12746 T12739 arg2Of position,(
R10004 T12746 T12740 arg1Of position,homologous
R10005 T12746 T12745 arg1Of position,approximate
R10006 T12746 T12747 arg2Of position,noted
R10007 T12747 T12748 arg1Of noted,in
R10008 T12749 T12748 arg2Of Fig.,in
R10009 T12749 T12750 arg1Of Fig.,1
R10010 T12751 T12739 arg3Of ),(
R10011 T12752 T12737 arg2Of and,were
R10012 T12753 T12752 arg2Of 5′-caatccatcttgttcagccat-3′,and
R10013 T12753 T12754 arg1Of 5′-caatccatcttgttcagccat-3′,(
R10014 T12757 T12754 arg2Of sequence,(
R10015 T12757 T12755 arg1Of sequence,homologous
R10016 T12757 T12756 arg1Of sequence,to
R10017 T12757 T12758 arg1Of sequence,in
R10018 T12759 T12758 arg2Of NeoR,in
R10019 T12760 T12754 arg3Of ),(
R10020 T12761 T12762 arg2Of Clones,in
R10021 T12761 T12763 arg1Of Clones,which
R10022 T12761 T12771 arg1Of Clones,were
R10023 T12761 T12773 arg2Of Clones,transfected
R10024 T12761 T12784 arg1Of Clones,remove
R10025 T12765 T12764 arg1Of copy,one
R10026 T12765 T12766 arg1Of copy,of
R10027 T12765 T12768 arg1Of copy,had
R10028 T12765 T12769 arg1Of copy,been
R10029 T12765 T12770 arg2Of copy,disrupted
R10030 T12767 T12766 arg2Of Rnf31,of
R10031 T12770 T12762 arg1Of disrupted,in
R10032 T12770 T12768 arg2Of disrupted,had
R10033 T12770 T12769 arg2Of disrupted,been
R10034 T12773 T12771 arg2Of transfected,were
R10035 T12773 T12772 arg1Of transfected,transiently
R10036 T12773 T12774 arg1Of transfected,with
R10037 T12773 T12780 arg1Of transfected,","
R10038 T12773 T12781 arg1Of transfected,in
R10039 T12777 T12774 arg2Of plasmid,with
R10040 T12777 T12775 arg1Of plasmid,an
R10041 T12777 T12776 arg1Of plasmid,expression
R10042 T12777 T12778 arg1Of plasmid,encoding
R10043 T12779 T12778 arg2Of Cre,encoding
R10044 T12782 T12781 arg2Of order,in
R10045 T12784 T12781 arg3Of remove,in
R10046 T12784 T12783 arg1Of remove,to
R10047 T12785 T12784 arg2Of NeoR,remove
R10048 T12787 T12786 arg1Of subclones,G418-sensitive
R10049 T12787 T12788 arg1Of subclones,were
R10050 T12787 T12789 arg2Of subclones,subjected
R10051 T12789 T12788 arg2Of subjected,were
R10052 T12789 T12790 arg1Of subjected,to
R10053 T12789 T12796 modOf subjected,to
R10054 T12793 T12790 arg2Of round,to
R10055 T12793 T12791 arg1Of round,a
R10056 T12793 T12792 arg1Of round,second
R10057 T12793 T12794 arg1Of round,of
R10058 T12795 T12794 arg2Of targeting,of
R10059 T12797 T12796 arg1Of disrupt,to
R10060 T12800 T12797 arg2Of copy,disrupt
R10061 T12800 T12798 arg1Of copy,the
R10062 T12800 T12799 arg1Of copy,remaining
R10063 T12800 T12801 arg1Of copy,of
R10064 T12802 T12801 arg2Of Rnf31,of
R10065 T12804 T12803 arg1Of clones,G418-resistant
R10066 T12804 T12805 arg1Of clones,were
R10067 T12804 T12806 arg2Of clones,tested
R10068 T12806 T12805 arg2Of tested,were
R10069 T12806 T12807 arg1Of tested,for
R10070 T12809 T12807 arg2Of recombination,for
R10071 T12809 T12808 arg1Of recombination,homologous
R10072 T12811 T12812 arg1Of PCR,and
R10073 T12813 T12812 arg2Of HOIP,and
R10074 T12815 T12806 arg1Of expression,tested
R10075 T12815 T12810 arg2Of expression,by
R10076 T12815 T12811 arg1Of expression,PCR
R10077 T12815 T12813 arg1Of expression,HOIP
R10078 T12815 T12814 arg1Of expression,protein
R10079 T12815 T12816 arg1Of expression,by
R10080 T12818 T12816 arg2Of blot,by
R10081 T12818 T12817 arg1Of blot,Western

2_test

Id Subject Object Predicate Lexical cue
21829693-12958312-89653801 148-149 12958312 denotes 7
21829693-17878362-89653802 153-154 17878362 denotes 8
21829693-12958312-89653803 734-735 12958312 denotes 7
21829693-12958312-89653804 926-927 12958312 denotes 7
T56054 148-149 12958312 denotes 7
T48514 153-154 17878362 denotes 8
T27828 734-735 12958312 denotes 7
T21517 926-927 12958312 denotes 7

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T12332 0-7 JJ denotes Somatic
T12333 8-12 NN denotes cell
T12334 13-17 NN denotes gene
T12335 18-27 VBG denotes targeting
T12336 28-31 DT denotes The
T12337 32-42 NN denotes generation
T12338 43-45 IN denotes of
T12339 46-60 JJ denotes HOIP-deficient
T12340 61-66 NNS denotes cells
T12341 67-70 VBD denotes was
T12342 71-83 VBN denotes accomplished
T12343 84-89 VBG denotes using
T12344 90-91 DT denotes a
T12345 92-102 RB denotes homologous
T12346 103-116 NN denotes recombination
T12347 117-125 NN denotes approach
T12348 126-135 VBD denotes described
T12349 136-146 RB denotes previously
T12350 147-148 JJ denotes [
T12351 148-149 CD denotes 7
T12352 149-150 NNP denotes ]
T12353 150-151 , denotes ,
T12354 152-153 NNP denotes [
T12355 153-154 CD denotes 8
T12356 154-155 NNP denotes ]
T12357 155-156 . denotes .
T12358 157-165 NNS denotes Segments
T12359 166-168 IN denotes of
T12360 169-174 JJ denotes Rnf31
T12361 175-179 NN denotes gene
T12362 180-188 NN denotes sequence
T12363 189-193 VBN denotes used
T12364 194-196 IN denotes in
T12365 197-200 DT denotes the
T12366 201-210 VBG denotes targeting
T12367 211-220 VB denotes construct
T12368 221-222 -LRB- denotes (
T12369 222-226 NNP denotes Fig.
T12370 227-229 NNP denotes 1A
T12371 229-230 -RRB- denotes )
T12372 231-235 VBD denotes were
T12373 236-245 VBN denotes amplified
T12374 246-248 IN denotes by
T12375 249-252 NNP denotes PCR
T12376 253-257 IN denotes from
T12377 258-261 NNP denotes A20
T12378 261-263 CD denotes .2
T12379 263-264 NNP denotes J
T12380 265-272 JJ denotes genomic
T12381 273-276 NN denotes DNA
T12382 276-277 . denotes .
T12383 278-281 DT denotes The
T12384 282-297 NN denotes oligonucleotide
T12385 298-305 NNS denotes primers
T12386 306-310 VBN denotes used
T12387 311-313 TO denotes to
T12388 314-322 VB denotes generate
T12389 323-326 DT denotes the
T12390 327-328 CD denotes 5
T12391 328-329 NN denotes ′
T12392 330-335 NN denotes flank
T12393 336-337 -LRB- denotes (
T12394 337-341 CD denotes 1224
T12395 342-344 NN denotes bp
T12396 344-345 -RRB- denotes )
T12397 346-350 VBD denotes were
T12398 351-352 CD denotes 5
T12399 352-353 SYM denotes ′
T12400 353-354 : denotes -
T12401 354-384 JJ denotes ttttctagagcggtggcttaagtgaccc-3
T12402 384-385 NN denotes ′
T12403 386-389 CC denotes and
T12404 390-391 CD denotes 5
T12405 391-392 SYM denotes ′
T12406 392-393 : denotes -
T12407 393-426 JJ denotes tattctagatgcagcatctgagaaagcaagc-3
T12408 426-427 NN denotes ′
T12409 427-428 . denotes .
T12410 429-432 DT denotes The
T12411 433-434 CD denotes 3
T12412 434-435 NN denotes ′
T12413 436-441 NN denotes flank
T12414 442-443 -LRB- denotes (
T12415 443-447 CD denotes 6032
T12416 448-450 NN denotes bp
T12417 450-451 -RRB- denotes )
T12418 452-459 NNS denotes primers
T12419 460-464 VBD denotes were
T12420 465-466 CD denotes 5
T12421 466-467 SYM denotes ′
T12422 467-468 : denotes -
T12423 468-509 JJ denotes aaaaccggtgtatgcttctttacgggagaaaaatattag-3
T12424 509-510 NN denotes ′
T12425 511-514 CC denotes and
T12426 515-516 CD denotes 5
T12427 516-517 SYM denotes ′
T12428 517-518 : denotes -
T12429 518-553 JJ denotes tataccggtatgaagccaaaggaacactgagag-3
T12430 553-554 NN denotes ′
T12431 554-555 . denotes .
T12432 556-567 NN denotes Restriction
T12433 568-580 NN denotes endonuclease
T12434 581-586 NNS denotes sites
T12435 587-589 IN denotes in
T12436 590-593 DT denotes the
T12437 594-609 NN denotes oligonucleotide
T12438 610-617 VBZ denotes primers
T12439 618-625 VBN denotes allowed
T12440 626-635 NN denotes insertion
T12441 636-638 IN denotes of
T12442 639-642 DT denotes the
T12443 643-646 NNP denotes PCR
T12444 647-655 NNS denotes products
T12445 656-660 IN denotes into
T12446 661-664 DT denotes the
T12447 665-674 VBG denotes targeting
T12448 675-681 NN denotes vector
T12449 681-682 . denotes .
T12450 683-686 CD denotes A20
T12451 686-688 CD denotes .2
T12452 688-689 NNP denotes J
T12453 690-695 NNS denotes cells
T12454 696-700 VBD denotes were
T12455 701-712 VBN denotes transfected
T12456 713-714 -LRB- denotes (
T12457 714-716 IN denotes by
T12458 717-732 NN denotes electroporation
T12459 733-734 NN denotes [
T12460 734-735 CD denotes 7
T12461 735-736 NNP denotes ]
T12462 736-737 -RRB- denotes )
T12463 738-742 IN denotes with
T12464 743-746 DT denotes the
T12465 747-756 VBG denotes targeting
T12466 757-766 VB denotes construct
T12467 767-770 CC denotes and
T12468 771-780 VBN denotes subcloned
T12469 781-783 IN denotes in
T12470 784-790 NN denotes medium
T12471 791-801 VBG denotes containing
T12472 802-805 CD denotes 600
T12473 806-808 JJ denotes µg
T12474 808-809 NN denotes /
T12475 809-811 NN denotes ml
T12476 812-816 NNP denotes G418
T12477 817-824 NN denotes sulfate
T12478 824-825 . denotes .
T12479 826-836 JJ denotes Homologous
T12480 837-850 NN denotes recombination
T12481 851-853 IN denotes in
T12482 854-868 JJ denotes G418-resistant
T12483 869-875 NNS denotes clones
T12484 876-879 VBD denotes was
T12485 880-888 VBN denotes detected
T12486 889-891 IN denotes by
T12487 892-895 NNP denotes PCR
T12488 896-898 IN denotes of
T12489 899-906 JJ denotes genomic
T12490 907-910 NN denotes DNA
T12491 910-911 , denotes ,
T12492 912-914 IN denotes as
T12493 915-924 VBN denotes described
T12494 925-926 NN denotes [
T12495 926-927 CD denotes 7
T12496 927-928 NNP denotes ]
T12497 928-929 . denotes .
T12498 930-945 NNP denotes Oligonucleotide
T12499 946-953 NNS denotes primers
T12500 954-958 VBD denotes used
T12501 959-962 IN denotes for
T12502 963-972 VBG denotes screening
T12503 973-977 VBD denotes were
T12504 978-979 CD denotes 5
T12505 979-980 SYM denotes ′
T12506 980-981 : denotes -
T12507 981-1009 JJ denotes cttcctgatctcagctttaccgtcac-3
T12508 1009-1010 NN denotes ′
T12509 1011-1012 -LRB- denotes (
T12510 1012-1022 RB denotes homologous
T12511 1023-1025 TO denotes to
T12512 1026-1033 JJ denotes genomic
T12513 1034-1042 NN denotes sequence
T12514 1042-1043 : denotes ;
T12515 1044-1055 JJ denotes approximate
T12516 1056-1064 NN denotes position
T12517 1065-1070 VBD denotes noted
T12518 1071-1073 IN denotes in
T12519 1074-1078 NNP denotes Fig.
T12520 1079-1080 CD denotes 1
T12521 1080-1081 -RRB- denotes )
T12522 1082-1085 CC denotes and
T12523 1086-1087 CD denotes 5
T12524 1087-1088 SYM denotes ′
T12525 1088-1089 : denotes -
T12526 1089-1112 JJ denotes caatccatcttgttcagccat-3
T12527 1112-1113 NN denotes ′
T12528 1114-1115 -LRB- denotes (
T12529 1115-1125 RB denotes homologous
T12530 1126-1128 TO denotes to
T12531 1129-1137 NN denotes sequence
T12532 1138-1140 IN denotes in
T12533 1141-1145 NNP denotes NeoR
T12534 1145-1146 -RRB- denotes )
T12535 1146-1147 . denotes .
T12536 1148-1154 NNS denotes Clones
T12537 1155-1157 IN denotes in
T12538 1158-1163 WDT denotes which
T12539 1164-1167 CD denotes one
T12540 1168-1172 NN denotes copy
T12541 1173-1175 IN denotes of
T12542 1176-1181 NNP denotes Rnf31
T12543 1182-1185 VBD denotes had
T12544 1186-1190 VBN denotes been
T12545 1191-1200 VBN denotes disrupted
T12546 1201-1205 VBD denotes were
T12547 1206-1217 RB denotes transiently
T12548 1218-1229 VBN denotes transfected
T12549 1230-1234 IN denotes with
T12550 1235-1237 DT denotes an
T12551 1238-1248 NN denotes expression
T12552 1249-1256 NN denotes plasmid
T12553 1257-1265 VBG denotes encoding
T12554 1266-1269 NNP denotes Cre
T12555 1269-1270 , denotes ,
T12556 1271-1273 IN denotes in
T12557 1274-1279 NN denotes order
T12558 1280-1282 TO denotes to
T12559 1283-1289 VB denotes remove
T12560 1290-1294 NNP denotes NeoR
T12561 1294-1295 . denotes .
T12562 1296-1310 JJ denotes G418-sensitive
T12563 1311-1320 NNS denotes subclones
T12564 1321-1325 VBD denotes were
T12565 1326-1335 VBN denotes subjected
T12566 1336-1338 TO denotes to
T12567 1339-1340 DT denotes a
T12568 1341-1347 JJ denotes second
T12569 1348-1353 NN denotes round
T12570 1354-1356 IN denotes of
T12571 1357-1366 VBG denotes targeting
T12572 1367-1369 TO denotes to
T12573 1370-1377 VB denotes disrupt
T12574 1378-1381 DT denotes the
T12575 1382-1391 VBG denotes remaining
T12576 1392-1396 NN denotes copy
T12577 1397-1399 IN denotes of
T12578 1400-1405 NNP denotes Rnf31
T12579 1405-1406 . denotes .
T12580 1407-1421 JJ denotes G418-resistant
T12581 1422-1428 NNS denotes clones
T12582 1429-1433 VBD denotes were
T12583 1434-1440 VBN denotes tested
T12584 1441-1444 IN denotes for
T12585 1445-1455 RB denotes homologous
T12586 1456-1469 NN denotes recombination
T12587 1470-1472 IN denotes by
T12588 1473-1476 NNP denotes PCR
T12589 1477-1480 CC denotes and
T12590 1481-1485 NNP denotes HOIP
T12591 1486-1493 NN denotes protein
T12592 1494-1504 NN denotes expression
T12593 1505-1507 IN denotes by
T12594 1508-1515 JJ denotes Western
T12595 1516-1520 NN denotes blot
T12596 1520-1521 . denotes .
R9585 T12332 T12334 amod Somatic,gene
R9586 T12333 T12334 compound cell,gene
R9587 T12334 T12342 nsubjpass gene,accomplished
R9588 T12335 T12334 acl targeting,gene
R9589 T12336 T12337 det The,generation
R9590 T12337 T12335 dobj generation,targeting
R9591 T12338 T12337 prep of,generation
R9592 T12339 T12340 amod HOIP-deficient,cells
R9593 T12340 T12338 pobj cells,of
R9594 T12341 T12342 auxpass was,accomplished
R9595 T12342 T12342 ROOT accomplished,accomplished
R9596 T12343 T12342 advcl using,accomplished
R9597 T12344 T12347 det a,approach
R9598 T12345 T12347 amod homologous,approach
R9599 T12346 T12347 compound recombination,approach
R9600 T12347 T12343 dobj approach,using
R9601 T12348 T12342 conj described,accomplished
R9602 T12349 T12350 advmod previously,[
R9603 T12350 T12352 amod [,]
R9604 T12351 T12352 nummod 7,]
R9605 T12352 T12348 dobj ],described
R9606 T12353 T12352 punct ",",]
R9607 T12354 T12356 nmod [,]
R9608 T12355 T12356 nummod 8,]
R9609 T12356 T12352 appos ],]
R9610 T12357 T12342 punct .,accomplished
R9611 T12358 T12367 nsubj Segments,construct
R9612 T12359 T12358 prep of,Segments
R9613 T12360 T12362 amod Rnf31,sequence
R9614 T12361 T12362 compound gene,sequence
R9615 T12362 T12359 pobj sequence,of
R9616 T12363 T12362 acl used,sequence
R9617 T12364 T12363 prep in,used
R9618 T12365 T12366 det the,targeting
R9619 T12366 T12364 pobj targeting,in
R9620 T12367 T12367 ROOT construct,construct
R9621 T12368 T12370 punct (,1A
R9622 T12369 T12370 compound Fig.,1A
R9623 T12370 T12367 dobj 1A,construct
R9624 T12371 T12367 punct ),construct
R9625 T12372 T12373 auxpass were,amplified
R9626 T12373 T12367 advcl amplified,construct
R9627 T12374 T12373 agent by,amplified
R9628 T12375 T12374 pobj PCR,by
R9629 T12376 T12373 prep from,amplified
R9630 T12377 T12379 nmod A20,J
R9631 T12378 T12379 nummod .2,J
R9632 T12379 T12381 nmod J,DNA
R9633 T12380 T12381 amod genomic,DNA
R9634 T12381 T12376 pobj DNA,from
R9635 T12382 T12367 punct .,construct
R9636 T12383 T12385 det The,primers
R9637 T12384 T12385 compound oligonucleotide,primers
R9638 T12385 T12397 nsubj primers,were
R9639 T12386 T12385 acl used,primers
R9640 T12387 T12388 aux to,generate
R9641 T12388 T12386 xcomp generate,used
R9642 T12389 T12392 det the,flank
R9643 T12390 T12392 nummod 5,flank
R9644 T12391 T12392 compound ′,flank
R9645 T12392 T12388 dobj flank,generate
R9646 T12393 T12392 punct (,flank
R9647 T12394 T12395 nummod 1224,bp
R9648 T12395 T12392 appos bp,flank
R9649 T12396 T12392 punct ),flank
R9650 T12397 T12397 ROOT were,were
R9651 T12398 T12401 nummod 5,ttttctagagcggtggcttaagtgaccc-3
R9652 T12399 T12401 punct ′,ttttctagagcggtggcttaagtgaccc-3
R9653 T12400 T12401 punct -,ttttctagagcggtggcttaagtgaccc-3
R9654 T12401 T12402 compound ttttctagagcggtggcttaagtgaccc-3,′
R9655 T12402 T12397 attr ′,were
R9656 T12403 T12402 cc and,′
R9657 T12404 T12407 nummod 5,tattctagatgcagcatctgagaaagcaagc-3
R9658 T12405 T12407 punct ′,tattctagatgcagcatctgagaaagcaagc-3
R9659 T12406 T12407 punct -,tattctagatgcagcatctgagaaagcaagc-3
R9660 T12407 T12408 amod tattctagatgcagcatctgagaaagcaagc-3,′
R9661 T12408 T12402 conj ′,′
R9662 T12409 T12397 punct .,were
R9663 T12410 T12413 det The,flank
R9664 T12411 T12412 nummod 3,′
R9665 T12412 T12413 compound ′,flank
R9666 T12413 T12419 nsubj flank,were
R9667 T12414 T12413 punct (,flank
R9668 T12415 T12413 appos 6032,flank
R9669 T12416 T12413 appos bp,flank
R9670 T12417 T12413 punct ),flank
R9671 T12418 T12419 nsubj primers,were
R9672 T12419 T12419 ROOT were,were
R9673 T12420 T12424 nummod 5,′
R9674 T12421 T12423 punct ′,aaaaccggtgtatgcttctttacgggagaaaaatattag-3
R9675 T12422 T12423 punct -,aaaaccggtgtatgcttctttacgggagaaaaatattag-3
R9676 T12423 T12424 compound aaaaccggtgtatgcttctttacgggagaaaaatattag-3,′
R9677 T12424 T12419 attr ′,were
R9678 T12425 T12424 cc and,′
R9679 T12426 T12429 nummod 5,tataccggtatgaagccaaaggaacactgagag-3
R9680 T12427 T12429 punct ′,tataccggtatgaagccaaaggaacactgagag-3
R9681 T12428 T12429 punct -,tataccggtatgaagccaaaggaacactgagag-3
R9682 T12429 T12430 compound tataccggtatgaagccaaaggaacactgagag-3,′
R9683 T12430 T12424 conj ′,′
R9684 T12431 T12419 punct .,were
R9685 T12432 T12434 compound Restriction,sites
R9686 T12433 T12434 compound endonuclease,sites
R9687 T12434 T12434 ROOT sites,sites
R9688 T12435 T12434 prep in,sites
R9689 T12436 T12438 det the,primers
R9690 T12437 T12438 compound oligonucleotide,primers
R9691 T12438 T12435 pobj primers,in
R9692 T12439 T12440 amod allowed,insertion
R9693 T12440 T12434 conj insertion,sites
R9694 T12441 T12440 prep of,insertion
R9695 T12442 T12444 det the,products
R9696 T12443 T12444 compound PCR,products
R9697 T12444 T12441 pobj products,of
R9698 T12445 T12434 prep into,sites
R9699 T12446 T12448 det the,vector
R9700 T12447 T12448 compound targeting,vector
R9701 T12448 T12445 pobj vector,into
R9702 T12449 T12434 punct .,sites
R9703 T12450 T12453 nummod A20,cells
R9704 T12451 T12453 nummod .2,cells
R9705 T12452 T12453 compound J,cells
R9706 T12453 T12455 nsubjpass cells,transfected
R9707 T12454 T12455 auxpass were,transfected
R9708 T12455 T12455 ROOT transfected,transfected
R9709 T12456 T12455 punct (,transfected
R9710 T12457 T12455 agent by,transfected
R9711 T12458 T12459 compound electroporation,[
R9712 T12459 T12457 pobj [,by
R9713 T12460 T12461 nummod 7,]
R9714 T12461 T12459 appos ],[
R9715 T12462 T12461 punct ),]
R9716 T12463 T12455 prep with,transfected
R9717 T12464 T12465 det the,targeting
R9718 T12465 T12463 pobj targeting,with
R9719 T12466 T12455 conj construct,transfected
R9720 T12467 T12466 cc and,construct
R9721 T12468 T12466 conj subcloned,construct
R9722 T12469 T12468 prep in,subcloned
R9723 T12470 T12469 pobj medium,in
R9724 T12471 T12470 acl containing,medium
R9725 T12472 T12476 nummod 600,G418
R9726 T12473 T12476 compound µg,G418
R9727 T12474 T12476 compound /,G418
R9728 T12475 T12476 compound ml,G418
R9729 T12476 T12477 compound G418,sulfate
R9730 T12477 T12471 dobj sulfate,containing
R9731 T12478 T12455 punct .,transfected
R9732 T12479 T12480 amod Homologous,recombination
R9733 T12480 T12485 nsubjpass recombination,detected
R9734 T12481 T12480 prep in,recombination
R9735 T12482 T12483 amod G418-resistant,clones
R9736 T12483 T12481 pobj clones,in
R9737 T12484 T12485 auxpass was,detected
R9738 T12485 T12485 ROOT detected,detected
R9739 T12486 T12485 agent by,detected
R9740 T12487 T12486 pobj PCR,by
R9741 T12488 T12487 prep of,PCR
R9742 T12489 T12490 amod genomic,DNA
R9743 T12490 T12488 pobj DNA,of
R9744 T12491 T12485 punct ",",detected
R9745 T12492 T12493 mark as,described
R9746 T12493 T12485 advcl described,detected
R9747 T12494 T12496 nmod [,]
R9748 T12495 T12496 nummod 7,]
R9749 T12496 T12493 dobj ],described
R9750 T12497 T12485 punct .,detected
R9751 T12498 T12499 compound Oligonucleotide,primers
R9752 T12499 T12503 nsubj primers,were
R9753 T12500 T12499 acl used,primers
R9754 T12501 T12500 prep for,used
R9755 T12502 T12501 pobj screening,for
R9756 T12503 T12517 ccomp were,noted
R9757 T12504 T12507 nummod 5,cttcctgatctcagctttaccgtcac-3
R9758 T12505 T12507 punct ′,cttcctgatctcagctttaccgtcac-3
R9759 T12506 T12507 punct -,cttcctgatctcagctttaccgtcac-3
R9760 T12507 T12508 amod cttcctgatctcagctttaccgtcac-3,′
R9761 T12508 T12503 attr ′,were
R9762 T12509 T12510 punct (,homologous
R9763 T12510 T12517 ccomp homologous,noted
R9764 T12511 T12510 prep to,homologous
R9765 T12512 T12513 amod genomic,sequence
R9766 T12513 T12511 pobj sequence,to
R9767 T12514 T12517 punct ;,noted
R9768 T12515 T12516 amod approximate,position
R9769 T12516 T12517 nsubj position,noted
R9770 T12517 T12517 ROOT noted,noted
R9771 T12518 T12517 prep in,noted
R9772 T12519 T12518 pobj Fig.,in
R9773 T12520 T12519 nummod 1,Fig.
R9774 T12521 T12517 punct ),noted
R9775 T12522 T12517 cc and,noted
R9776 T12523 T12526 nummod 5,caatccatcttgttcagccat-3
R9777 T12524 T12526 punct ′,caatccatcttgttcagccat-3
R9778 T12525 T12526 punct -,caatccatcttgttcagccat-3
R9779 T12526 T12527 compound caatccatcttgttcagccat-3,′
R9780 T12527 T12527 ROOT ′,′
R9781 T12528 T12529 punct (,homologous
R9782 T12529 T12527 parataxis homologous,′
R9783 T12530 T12531 aux to,sequence
R9784 T12531 T12529 relcl sequence,homologous
R9785 T12532 T12531 prep in,sequence
R9786 T12533 T12532 pobj NeoR,in
R9787 T12534 T12529 punct ),homologous
R9788 T12535 T12517 punct .,noted
R9789 T12536 T12548 nsubjpass Clones,transfected
R9790 T12537 T12545 prep in,disrupted
R9791 T12538 T12537 pobj which,in
R9792 T12539 T12540 nummod one,copy
R9793 T12540 T12545 nsubjpass copy,disrupted
R9794 T12541 T12540 prep of,copy
R9795 T12542 T12541 pobj Rnf31,of
R9796 T12543 T12545 aux had,disrupted
R9797 T12544 T12545 auxpass been,disrupted
R9798 T12545 T12536 relcl disrupted,Clones
R9799 T12546 T12548 auxpass were,transfected
R9800 T12547 T12548 advmod transiently,transfected
R9801 T12548 T12548 ROOT transfected,transfected
R9802 T12549 T12548 prep with,transfected
R9803 T12550 T12552 det an,plasmid
R9804 T12551 T12552 compound expression,plasmid
R9805 T12552 T12549 pobj plasmid,with
R9806 T12553 T12554 compound encoding,Cre
R9807 T12554 T12548 npadvmod Cre,transfected
R9808 T12555 T12548 punct ",",transfected
R9809 T12556 T12548 prep in,transfected
R9810 T12557 T12556 pobj order,in
R9811 T12558 T12559 aux to,remove
R9812 T12559 T12557 acl remove,order
R9813 T12560 T12559 dobj NeoR,remove
R9814 T12561 T12548 punct .,transfected
R9815 T12562 T12563 amod G418-sensitive,subclones
R9816 T12563 T12565 nsubjpass subclones,subjected
R9817 T12564 T12565 auxpass were,subjected
R9818 T12565 T12565 ROOT subjected,subjected
R9819 T12566 T12565 prep to,subjected
R9820 T12567 T12569 det a,round
R9821 T12568 T12569 amod second,round
R9822 T12569 T12566 pobj round,to
R9823 T12570 T12569 prep of,round
R9824 T12571 T12570 pobj targeting,of
R9825 T12572 T12573 aux to,disrupt
R9826 T12573 T12571 xcomp disrupt,targeting
R9827 T12574 T12576 det the,copy
R9828 T12575 T12576 amod remaining,copy
R9829 T12576 T12573 dobj copy,disrupt
R9830 T12577 T12576 prep of,copy
R9831 T12578 T12577 pobj Rnf31,of
R9832 T12579 T12565 punct .,subjected
R9833 T12580 T12581 amod G418-resistant,clones
R9834 T12581 T12583 nsubjpass clones,tested
R9835 T12582 T12583 auxpass were,tested
R9836 T12583 T12583 ROOT tested,tested
R9837 T12584 T12583 prep for,tested
R9838 T12585 T12586 amod homologous,recombination
R9839 T12586 T12584 pobj recombination,for
R9840 T12587 T12586 prep by,recombination
R9841 T12588 T12587 pobj PCR,by
R9842 T12589 T12588 cc and,PCR
R9843 T12590 T12592 compound HOIP,expression
R9844 T12591 T12592 compound protein,expression
R9845 T12592 T12583 dobj expression,tested
R9846 T12593 T12583 agent by,tested
R9847 T12594 T12595 amod Western,blot
R9848 T12595 T12593 pobj blot,by
R9849 T12596 T12583 punct .,tested

GO-BP

Id Subject Object Predicate Lexical cue
T12839 817-824 http://purl.obolibrary.org/obo/GO_0051923 denotes sulfate

GO-CC

Id Subject Object Predicate Lexical cue
T12840 8-12 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T12841 61-66 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T12842 690-695 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

sentences

Id Subject Object Predicate Lexical cue
T12320 0-27 Sentence denotes Somatic cell gene targeting
T12321 28-156 Sentence denotes The generation of HOIP-deficient cells was accomplished using a homologous recombination approach described previously [7], [8].
T12322 157-277 Sentence denotes Segments of Rnf31 gene sequence used in the targeting construct (Fig. 1A) were amplified by PCR from A20.2J genomic DNA.
T12323 278-428 Sentence denotes The oligonucleotide primers used to generate the 5′ flank (1224 bp) were 5′-ttttctagagcggtggcttaagtgaccc-3′ and 5′-tattctagatgcagcatctgagaaagcaagc-3′.
T12324 429-555 Sentence denotes The 3′ flank (6032 bp) primers were 5′-aaaaccggtgtatgcttctttacgggagaaaaatattag-3′ and 5′-tataccggtatgaagccaaaggaacactgagag-3′.
T12325 556-682 Sentence denotes Restriction endonuclease sites in the oligonucleotide primers allowed insertion of the PCR products into the targeting vector.
T12326 683-825 Sentence denotes A20.2J cells were transfected (by electroporation [7]) with the targeting construct and subcloned in medium containing 600 µg/ml G418 sulfate.
T12327 826-929 Sentence denotes Homologous recombination in G418-resistant clones was detected by PCR of genomic DNA, as described [7].
T12328 930-1147 Sentence denotes Oligonucleotide primers used for screening were 5′-cttcctgatctcagctttaccgtcac-3′ (homologous to genomic sequence; approximate position noted in Fig. 1) and 5′-caatccatcttgttcagccat-3′ (homologous to sequence in NeoR).
T12329 1148-1295 Sentence denotes Clones in which one copy of Rnf31 had been disrupted were transiently transfected with an expression plasmid encoding Cre, in order to remove NeoR.
T12330 1296-1406 Sentence denotes G418-sensitive subclones were subjected to a second round of targeting to disrupt the remaining copy of Rnf31.
T12331 1407-1521 Sentence denotes G418-resistant clones were tested for homologous recombination by PCR and HOIP protein expression by Western blot.
T191 0-27 Sentence denotes Somatic cell gene targeting
T192 28-156 Sentence denotes The generation of HOIP-deficient cells was accomplished using a homologous recombination approach described previously [7], [8].
T193 157-277 Sentence denotes Segments of Rnf31 gene sequence used in the targeting construct (Fig. 1A) were amplified by PCR from A20.2J genomic DNA.
T194 278-428 Sentence denotes The oligonucleotide primers used to generate the 5′ flank (1224 bp) were 5′-ttttctagagcggtggcttaagtgaccc-3′ and 5′-tattctagatgcagcatctgagaaagcaagc-3′.
T195 429-555 Sentence denotes The 3′ flank (6032 bp) primers were 5′-aaaaccggtgtatgcttctttacgggagaaaaatattag-3′ and 5′-tataccggtatgaagccaaaggaacactgagag-3′.
T196 556-682 Sentence denotes Restriction endonuclease sites in the oligonucleotide primers allowed insertion of the PCR products into the targeting vector.
T197 683-825 Sentence denotes A20.2J cells were transfected (by electroporation [7]) with the targeting construct and subcloned in medium containing 600 µg/ml G418 sulfate.
T198 826-929 Sentence denotes Homologous recombination in G418-resistant clones was detected by PCR of genomic DNA, as described [7].
T199 930-1147 Sentence denotes Oligonucleotide primers used for screening were 5′-cttcctgatctcagctttaccgtcac-3′ (homologous to genomic sequence; approximate position noted in Fig. 1) and 5′-caatccatcttgttcagccat-3′ (homologous to sequence in NeoR).
T200 1148-1295 Sentence denotes Clones in which one copy of Rnf31 had been disrupted were transiently transfected with an expression plasmid encoding Cre, in order to remove NeoR.
T201 1296-1406 Sentence denotes G418-sensitive subclones were subjected to a second round of targeting to disrupt the remaining copy of Rnf31.
T202 1407-1521 Sentence denotes G418-resistant clones were tested for homologous recombination by PCR and HOIP protein expression by Western blot.

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T12819 46-50 Q96EP0 denotes HOIP
T12820 169-174 Q96EP0 denotes Rnf31
T12821 258-261 P21580 denotes A20
T12822 683-686 P21580 denotes A20
T12823 1176-1181 Q96EP0 denotes Rnf31
T12824 1266-1269 P06956 denotes Cre
T12825 1400-1405 Q96EP0 denotes Rnf31
T12826 1481-1485 Q96EP0 denotes HOIP

test2

Id Subject Object Predicate Lexical cue Speculation
T12303 46-50 Protein denotes HOIP
T12304 51-60 Negative_regulation denotes deficient
T12305 169-174 Protein denotes Rnf31 true
T12306 1176-1181 Protein denotes Rnf31
T12307 1238-1248 Gene_expression denotes expression
T12308 1370-1377 Negative_regulation denotes disrupt
T12309 1400-1405 Protein denotes Rnf31
T12310 1481-1485 Protein denotes HOIP
T12311 1494-1504 Gene_expression denotes expression
R9580 T12303 T12304 themeOf HOIP,deficient
R9581 T12310 T12311 themeOf HOIP,expression