PMC:3148254 / 27686-34300 JSON TXT

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bionlp-st-ge-2016-reference

Id	Subject	Object	Predicate	Lexical cue	Speculation
T11918	223-233	Gene_expression	denotes	expressing
T11919	234-239	Protein	denotes	CD154
T12312	930-934	Protein	denotes	HOIP
T12313	935-944	Negative_regulation	denotes	deficient
T12314	1053-1058	Protein	denotes	Rnf31
T12315	2060-2065	Protein	denotes	Rnf31
T12316	2075-2084	Negative_regulation	denotes	disrupted
T12317	2284-2289	Protein	denotes	Rnf31
T12318	2365-2369	Protein	denotes	HOIP
T12319	2378-2388	Gene_expression	denotes	expression	true
T12894	2509-2513	Protein	denotes	HOIP
T12895	2518-2522	Protein	denotes	IKKγ
T12896	2713-2717	Protein	denotes	HOIP
T12897	2808-2812	Protein	denotes	IKKγ
T12898	2979-2983	Protein	denotes	HOIP
T12899	2993-2997	Protein	denotes	IKKγ
T12900	3126-3130	Protein	denotes	HOIP
T12901	3131-3140	Negative_regulation	denotes	deficient
T13342	3457-3469	Positive_regulation	denotes	upregulation
T13550	3858-3861	Protein	denotes	GLε
T13551	3883-3887	Protein	denotes	CD40
T13552	3888-3897	Positive_regulation	denotes	simulated	true
T13553	3898-3908	Positive_regulation	denotes	activation
T13554	3912-3915	Protein	denotes	GLε
T13555	3916-3929	Transcription	denotes	transcription
T13556	4118-4122	Protein	denotes	IL-4
T13557	4265-4268	Protein	denotes	GLε
T13558	4273-4278	Protein	denotes	Hprt1
T13559	4407-4417	Gene_expression	denotes	Expression
T13560	4421-4424	Protein	denotes	GLε
T13561	4462-4472	Gene_expression	denotes	expression
T13562	4476-4481	Protein	denotes	Hprt1
T13864	4534-4538	Protein	denotes	CD40
T13865	4593-4603	Gene_expression	denotes	expressing
T13866	4610-4615	Protein	denotes	CD154
T13867	4617-4628	Protein	denotes	CD40 ligand
T14274	5450-5454	Protein	denotes	CD40
T13341	3452-3456	Protein	denotes	CD80
R9245	T11919	T11918	themeOf	CD154,expressing
R9582	T12312	T12313	themeOf	HOIP,deficient
R9583	T12315	T12316	themeOf	Rnf31,disrupted
R9584	T12318	T12319	themeOf	HOIP,expression
R10096	T12900	T12901	themeOf	HOIP,deficient
R10479	T13341	T13342	themeOf	CD80,upregulation
R10647	T13551	T13552	causeOf	CD40,simulated
R10648	T13553	T13552	themeOf	activation,simulated
R10649	T13554	T13555	themeOf	GLε,transcription
R10650	T13555	T13553	themeOf	transcription,activation
R10651	T13560	T13559	themeOf	GLε,Expression
R10652	T13562	T13561	themeOf	Hprt1,expression
R10898	T13866	T13865	themeOf	CD154,expressing
R10899	T13867	T13866	equivalentTo	CD40 ligand,CD154

bionlp-st-ge-2016-reference-tees

Id	Subject	Object	Predicate	Lexical cue
T12050	234-239	Protein	denotes	CD154
T12051	223-233	Gene_expression	denotes	expressing
T12290	278-312	Protein	denotes	Mouse anti-birch profilin antibody
T12291	340-344	Protein	denotes	CD40
T12292	529-544	Protein	denotes	anti-mouse CD80
T12293	725-742	Protein	denotes	Goat anti-rat IgG
T12294	748-751	Protein	denotes	HRP
T12859	1053-1058	Protein	denotes	Rnf31
T12860	2025-2029	Protein	denotes	NeoR
T12861	2060-2065	Protein	denotes	Rnf31
T12862	2150-2153	Protein	denotes	Cre
T12863	2174-2178	Protein	denotes	NeoR
T12864	2122-2132	Gene_expression	denotes	expression
T12865	2284-2289	Protein	denotes	Rnf31
T12866	2357-2360	Protein	denotes	PCR
T12867	2365-2377	Protein	denotes	HOIP protein
T12868	2378-2388	Gene_expression	denotes	expression
T12869	2378-2388	Gene_expression	denotes	expression
T13321	2509-2538	Protein	denotes	HOIP and IKKγ cDNA constructs
T13322	2587-2590	Protein	denotes	GFP
T13323	2564-2573	Negative_regulation	denotes	replacing
T13324	2707-2722	Protein	denotes	mouse HOIP cDNA
T13325	2776-2792	Protein	denotes	A pMIP construct
T13326	2802-2812	Protein	denotes	mouse IKKγ
T13327	2864-2867	Protein	denotes	tag
T13328	2988-2997	Protein	denotes	pMIP-IKKγ
T13519	3452-3456	Protein	denotes	CD80
T13520	3457-3469	Positive_regulation	denotes	upregulation
T13521	3572-3576	Protein	denotes	CD40
T13522	3583-3607	Protein	denotes	isotype control antibody
T13838	3883-3887	Protein	denotes	CD40
T13839	4030-4034	Protein	denotes	CD40
T13840	4273-4278	Protein	denotes	Hprt1
T13841	4476-4481	Protein	denotes	Hprt1
T13842	4462-4472	Gene_expression	denotes	expression
T14258	4484-4489	Protein	denotes	NF-κB
T14259	4494-4497	Protein	denotes	JNK
T14260	4498-4508	Positive_regulation	denotes	activation
T14261	4534-4538	Protein	denotes	CD40
T14262	4610-4615	Protein	denotes	CD154
T14263	4617-4621	Protein	denotes	CD40
T14264	4593-4603	Gene_expression	denotes	expressing
T14265	4593-4603	Gene_expression	denotes	expressing
T14266	4806-4809	Protein	denotes	Fig
T14267	5176-5179	Protein	denotes	Fig
T14732	5450-5454	Protein	denotes	CD40
T14733	5696-5708	Protein	denotes	anti-rat IgG
T14734	5710-5717	Protein	denotes	Jackson
T14735	5735-5739	Protein	denotes	CD40
T14736	5741-5745	Protein	denotes	1C10
T14737	6029-6047	Protein	denotes	lambda phosphatase
T14738	6007-6023	Phosphorylation	denotes	dephosphorylated
R9362	T12050	T12051	themeOf	CD154,expressing
R10088	T12862	T12864	themeOf	Cre,expression
R10089	T12866	T12868	themeOf	PCR,expression
R10090	T12867	T12869	themeOf	HOIP protein,expression
R10475	T13322	T13323	themeOf	GFP,replacing
R10634	T13519	T13520	themeOf	CD80,upregulation
R10887	T13841	T13842	themeOf	Hprt1,expression
R11252	T14258	T14260	themeOf	NF-κB,activation
R11253	T14262	T14264	themeOf	CD154,expressing
R11254	T14263	T14265	themeOf	CD40,expressing
R11682	T14737	T14738	themeOf	lambda phosphatase,dephosphorylated

events-check-again

Id	Subject	Object	Predicate	Lexical cue	Speculation
T12052	223-233	Gene_expression	denotes	expressing
T12053	234-239	Protein	denotes	CD154
T12870	930-934	Protein	denotes	HOIP
T12871	935-944	Negative_regulation	denotes	deficient
T12872	1053-1058	Protein	denotes	Rnf31
T12873	2060-2065	Protein	denotes	Rnf31
T12874	2075-2084	Negative_regulation	denotes	disrupted
T12875	2284-2289	Protein	denotes	Rnf31
T12876	2365-2369	Protein	denotes	HOIP
T12877	2378-2388	Gene_expression	denotes	expression	true
T13329	2509-2513	Protein	denotes	HOIP
T13330	2518-2522	Protein	denotes	IKKγ
T13331	2713-2717	Protein	denotes	HOIP
T13332	2808-2812	Protein	denotes	IKKγ
T13333	2979-2983	Protein	denotes	HOIP
T13334	2993-2997	Protein	denotes	IKKγ
T13335	3126-3130	Protein	denotes	HOIP
T13336	3131-3140	Negative_regulation	denotes	deficient
T13523	3452-3456	Protein	denotes	CD80
T13524	3457-3469	Positive_regulation	denotes	upregulation
T13843	3858-3861	Protein	denotes	GLε
T13844	3883-3887	Protein	denotes	CD40
T13845	3888-3897	Positive_regulation	denotes	simulated	true
T13846	3898-3908	Positive_regulation	denotes	activation
T13847	3912-3915	Protein	denotes	GLε
T13848	3916-3929	Transcription	denotes	transcription
T13849	4118-4122	Protein	denotes	IL-4
T13850	4265-4268	Protein	denotes	GLε
T13851	4273-4278	Protein	denotes	Hprt1
T13852	4407-4417	Gene_expression	denotes	Expression
T13853	4421-4424	Protein	denotes	GLε
T13854	4462-4472	Gene_expression	denotes	expression
T13855	4476-4481	Protein	denotes	Hprt1
T14268	4534-4538	Protein	denotes	CD40
T14269	4593-4603	Gene_expression	denotes	expressing
T14270	4610-4615	Protein	denotes	CD154
T14271	4617-4628	Protein	denotes	CD40 ligand
T14739	5450-5454	Protein	denotes	CD40
R9363	T12053	T12052	themeOf	CD154,expressing
R10091	T12870	T12871	themeOf	HOIP,deficient
R10092	T12873	T12874	themeOf	Rnf31,disrupted
R10093	T12876	T12877	themeOf	HOIP,expression
R10476	T13335	T13336	themeOf	HOIP,deficient
R10635	T13523	T13524	themeOf	CD80,upregulation
R10888	T13844	T13845	causeOf	CD40,simulated
R10889	T13846	T13845	themeOf	activation,simulated
R10890	T13847	T13848	themeOf	GLε,transcription
R10891	T13848	T13846	themeOf	transcription,activation
R10892	T13853	T13852	themeOf	GLε,Expression
R10893	T13855	T13854	themeOf	Hprt1,expression
R11255	T14270	T14269	themeOf	CD154,expressing
R11256	T14271	T14270	equivalentTo	CD40 ligand,CD154

pmc-enju-pas

Id	Subject	Object	Predicate	Lexical cue
T11983	23-27	NN	denotes	Cell
T11984	28-33	NN	denotes	lines
T11985	34-37	DT	denotes	The
T11986	38-43	NN	denotes	mouse
T11987	44-45	NN	denotes	B
T11988	46-50	NN	denotes	cell
T11989	51-55	NN	denotes	line
T11990	56-62	NN	denotes	A20.2J
T11991	63-66	VB	denotes	has
T11992	67-71	VB	denotes	been
T11993	72-82	RB	denotes	previously
T11994	83-92	VB	denotes	described
T11995	93-94	-LRB-	denotes	[
T11996	94-96	CD	denotes	26
T11997	96-97	-RRB-	denotes	]
T11998	97-98	-COMMA-	denotes	,
T11999	99-100	-LRB-	denotes	[
T12000	100-102	CD	denotes	27
T12001	102-103	-RRB-	denotes	]
T12002	105-110	NN	denotes	Cells
T12003	111-115	VB	denotes	were
T12004	116-121	VB	denotes	grown
T12005	122-124	IN	denotes	in
T12006	125-129	NN	denotes	RPMI
T12007	130-134	CD	denotes	1640
T12008	134-135	-COMMA-	denotes	,
T12009	136-138	CD	denotes	10
T12010	138-139	NN	denotes	%
T12011	140-143	NN	denotes	FCS
T12012	143-144	-COMMA-	denotes	,
T12013	145-147	CD	denotes	10
T12014	148-150	NN	denotes	µM
T12015	151-155	NN	denotes	2-ME
T12016	155-156	-COMMA-	denotes	,
T12017	157-158	CD	denotes	2
T12018	159-161	NN	denotes	mM
T12019	162-173	NN	denotes	L-glutamine
T12020	173-174	-COMMA-	denotes	,
T12021	175-178	CC	denotes	and
T12022	179-190	NN	denotes	antibiotics
T12023	192-196	NN	denotes	HI-5
T12024	197-203	NN	denotes	insect
T12025	204-209	NN	denotes	cells
T12026	210-211	-LRB-	denotes	(
T12027	211-221	NN	denotes	Invitrogen
T12028	221-222	-RRB-	denotes	)
T12029	223-233	VB	denotes	expressing
T12030	234-239	NN	denotes	CD154
T12031	240-244	VB	denotes	have
T12032	245-249	VB	denotes	been
T12033	250-259	VB	denotes	described
T12034	260-261	-LRB-	denotes	[
T12035	261-263	CD	denotes	28
T12036	263-264	-RRB-	denotes	]
T12163	267-277	NN	denotes	Antibodies
T12164	278-283	NN	denotes	Mouse
T12165	284-294	JJ	denotes	anti-birch
T12166	295-303	NN	denotes	profilin
T12167	304-312	NN	denotes	antibody
T12168	313-314	-LRB-	denotes	(
T12169	314-317	NN	denotes	4A6
T12170	318-319	-LRB-	denotes	[
T12171	319-321	CD	denotes	29
T12172	321-322	-RRB-	denotes	]
T12173	322-323	-RRB-	denotes	)
T12174	323-324	-COMMA-	denotes	,
T12175	325-328	NN	denotes	rat
T12176	329-339	JJ	denotes	anti-mouse
T12177	340-344	NN	denotes	CD40
T12178	345-346	-LRB-	denotes	(
T12179	346-350	CD	denotes	1C10
T12180	351-352	-LRB-	denotes	[
T12181	352-354	CD	denotes	30
T12182	354-355	-RRB-	denotes	]
T12183	355-356	-RRB-	denotes	)
T12184	357-360	CC	denotes	and
T12185	361-362	DT	denotes	a
T12186	363-366	NN	denotes	rat
T12187	367-374	NN	denotes	isotype
T12188	375-382	NN	denotes	control
T12189	383-391	NN	denotes	antibody
T12190	392-393	-LRB-	denotes	(
T12191	393-398	NN	denotes	mAb72
T12192	398-399	-COMMA-	denotes	,
T12193	400-413	NNP	denotes	Developmental
T12194	414-421	NNP	denotes	Studies
T12195	422-431	NNP	denotes	Hybridoma
T12196	432-436	NNP	denotes	Bank
T12197	436-437	-COMMA-	denotes	,
T12198	438-448	NNP	denotes	University
T12199	449-451	IN	denotes	of
T12200	452-456	NNP	denotes	Iowa
T12201	456-457	-COMMA-	denotes	,
T12202	458-462	NNP	denotes	Iowa
T12203	463-467	NNP	denotes	City
T12204	467-468	-COMMA-	denotes	,
T12205	469-471	NN	denotes	IA
T12206	471-472	-RRB-	denotes	)
T12207	473-477	VB	denotes	were
T12208	478-486	VB	denotes	isolated
T12209	487-491	IN	denotes	from
T12210	492-501	NN	denotes	hybridoma
T12211	502-514	NN	denotes	supernatants
T12212	516-528	JJ	denotes	FITC-labeled
T12213	529-539	JJ	denotes	anti-mouse
T12214	540-544	NN	denotes	CD80
T12215	545-548	CC	denotes	and
T12216	549-551	DT	denotes	an
T12217	552-559	NN	denotes	isotype
T12218	560-567	NN	denotes	control
T12219	568-570	NN	denotes	Ab
T12220	571-575	VB	denotes	were
T12221	576-580	IN	denotes	from
T12222	581-593	NN	denotes	eBiosciences
T12223	595-601	NN	denotes	Rabbit
T12224	602-611	JJ	denotes	anti-HOIP
T12225	612-614	NN	denotes	Ab
T12226	615-616	-LRB-	denotes	[
T12227	616-617	CD	denotes	6
T12228	617-618	-RRB-	denotes	]
T12229	619-622	VB	denotes	was
T12230	623-626	DT	denotes	the
T12231	627-631	NN	denotes	kind
T12232	632-636	NN	denotes	gift
T12233	637-639	IN	denotes	of
T12234	640-643	NNP	denotes	Dr.
T12235	644-649	NNP	denotes	Betty
T12236	650-656	NNP	denotes	Eipper
T12237	657-658	-LRB-	denotes	(
T12238	658-668	NNP	denotes	University
T12239	669-671	IN	denotes	of
T12240	672-683	NNP	denotes	Connecticut
T12241	684-690	NNP	denotes	Health
T12242	691-697	NNP	denotes	Center
T12243	697-698	-COMMA-	denotes	,
T12244	699-709	NNP	denotes	Farmington
T12245	709-710	-COMMA-	denotes	,
T12246	711-722	NNP	denotes	Connecticut
T12247	722-723	-RRB-	denotes	)
T12248	725-729	NNP	denotes	Goat
T12249	730-738	JJ	denotes	anti-rat
T12250	739-742	NN	denotes	IgG
T12251	742-743	-COMMA-	denotes	,
T12252	744-747	CC	denotes	and
T12253	748-759	JJ	denotes	HRP-labeled
T12254	760-769	JJ	denotes	secondary
T12255	770-773	NN	denotes	Abs
T12256	774-778	VB	denotes	were
T12257	779-783	IN	denotes	from
T12258	784-791	NNP	denotes	Jackson
T12259	792-806	NNP	denotes	ImmunoResearch
T12260	807-819	NNP	denotes	Laboratories
T12261	819-820	-COMMA-	denotes	,
T12262	821-825	NNP	denotes	Inc.
T12263	826-829	DT	denotes	All
T12264	830-835	JJ	denotes	other
T12265	836-846	NN	denotes	antibodies
T12266	847-851	VB	denotes	used
T12267	852-856	VB	denotes	were
T12268	857-866	VB	denotes	described
T12269	867-877	RB	denotes	previously
T12270	878-879	-LRB-	denotes	[
T12271	879-880	CD	denotes	6
T12272	880-881	-RRB-	denotes	]
T12597	884-891	JJ	denotes	Somatic
T12598	892-896	NN	denotes	cell
T12599	897-901	NN	denotes	gene
T12600	902-911	VB	denotes	targeting
T12601	912-915	DT	denotes	The
T12602	916-926	NN	denotes	generation
T12603	927-929	IN	denotes	of
T12604	930-944	JJ	denotes	HOIP-deficient
T12605	945-950	NN	denotes	cells
T12606	951-954	VB	denotes	was
T12607	955-967	VB	denotes	accomplished
T12608	968-973	VB	denotes	using
T12609	974-975	DT	denotes	a
T12610	976-986	JJ	denotes	homologous
T12611	987-1000	NN	denotes	recombination
T12612	1001-1009	NN	denotes	approach
T12613	1010-1019	VB	denotes	described
T12614	1020-1030	RB	denotes	previously
T12615	1031-1032	-LRB-	denotes	[
T12616	1032-1033	CD	denotes	7
T12617	1033-1034	-RRB-	denotes	]
T12618	1034-1035	-COMMA-	denotes	,
T12619	1036-1037	-LRB-	denotes	[
T12620	1037-1038	CD	denotes	8
T12621	1038-1039	-RRB-	denotes	]
T12622	1041-1049	NN	denotes	Segments
T12623	1050-1052	IN	denotes	of
T12624	1053-1058	NN	denotes	Rnf31
T12625	1059-1063	NN	denotes	gene
T12626	1064-1072	NN	denotes	sequence
T12627	1073-1077	VB	denotes	used
T12628	1078-1080	IN	denotes	in
T12629	1081-1084	DT	denotes	the
T12630	1085-1094	VB	denotes	targeting
T12631	1095-1104	NN	denotes	construct
T12632	1105-1106	-LRB-	denotes	(
T12633	1106-1110	NN	denotes	Fig.
T12634	1111-1113	NN	denotes	1A
T12635	1113-1114	-RRB-	denotes	)
T12636	1115-1119	VB	denotes	were
T12637	1120-1129	VB	denotes	amplified
T12638	1130-1132	IN	denotes	by
T12639	1133-1136	NN	denotes	PCR
T12640	1137-1141	IN	denotes	from
T12641	1142-1148	NN	denotes	A20.2J
T12642	1149-1156	JJ	denotes	genomic
T12643	1157-1160	NN	denotes	DNA
T12644	1162-1165	DT	denotes	The
T12645	1166-1181	NN	denotes	oligonucleotide
T12646	1182-1189	NN	denotes	primers
T12647	1190-1194	VB	denotes	used
T12648	1195-1197	TO	denotes	to
T12649	1198-1206	VB	denotes	generate
T12650	1207-1210	DT	denotes	the
T12651	1211-1213	NN	denotes	5′
T12652	1214-1219	NN	denotes	flank
T12653	1220-1221	-LRB-	denotes	(
T12654	1221-1225	CD	denotes	1224
T12655	1226-1228	NN	denotes	bp
T12656	1228-1229	-RRB-	denotes	)
T12657	1230-1234	VB	denotes	were
T12658	1235-1269	JJ	denotes	5′-ttttctagagcggtggcttaagtgaccc-3′
T12659	1270-1273	CC	denotes	and
T12660	1274-1311	JJ	denotes	5′-tattctagatgcagcatctgagaaagcaagc-3′
T12661	1313-1316	DT	denotes	The
T12662	1317-1319	NN	denotes	3′
T12663	1320-1325	NN	denotes	flank
T12664	1326-1327	-LRB-	denotes	(
T12665	1327-1331	CD	denotes	6032
T12666	1332-1334	NN	denotes	bp
T12667	1334-1335	-RRB-	denotes	)
T12668	1336-1343	NN	denotes	primers
T12669	1344-1348	VB	denotes	were
T12670	1349-1394	JJ	denotes	5′-aaaaccggtgtatgcttctttacgggagaaaaatattag-3′
T12671	1395-1398	CC	denotes	and
T12672	1399-1438	JJ	denotes	5′-tataccggtatgaagccaaaggaacactgagag-3′
T12673	1440-1451	NN	denotes	Restriction
T12674	1452-1464	NN	denotes	endonuclease
T12675	1465-1470	NN	denotes	sites
T12676	1471-1473	IN	denotes	in
T12677	1474-1477	DT	denotes	the
T12678	1478-1493	NN	denotes	oligonucleotide
T12679	1494-1501	NN	denotes	primers
T12680	1502-1509	VB	denotes	allowed
T12681	1510-1519	NN	denotes	insertion
T12682	1520-1522	IN	denotes	of
T12683	1523-1526	DT	denotes	the
T12684	1527-1530	NN	denotes	PCR
T12685	1531-1539	NN	denotes	products
T12686	1540-1544	IN	denotes	into
T12687	1545-1548	DT	denotes	the
T12688	1549-1558	NN	denotes	targeting
T12689	1559-1565	NN	denotes	vector
T12690	1567-1573	NN	denotes	A20.2J
T12691	1574-1579	NN	denotes	cells
T12692	1580-1584	VB	denotes	were
T12693	1585-1596	VB	denotes	transfected
T12694	1597-1598	-LRB-	denotes	(
T12695	1598-1600	IN	denotes	by
T12696	1601-1616	NN	denotes	electroporation
T12697	1617-1618	-LRB-	denotes	[
T12698	1618-1619	CD	denotes	7
T12699	1619-1620	-RRB-	denotes	]
T12700	1620-1621	-RRB-	denotes	)
T12701	1622-1626	IN	denotes	with
T12702	1627-1630	DT	denotes	the
T12703	1631-1640	VB	denotes	targeting
T12704	1641-1650	NN	denotes	construct
T12705	1651-1654	CC	denotes	and
T12706	1655-1664	VB	denotes	subcloned
T12707	1665-1667	IN	denotes	in
T12708	1668-1674	NN	denotes	medium
T12709	1675-1685	VB	denotes	containing
T12710	1686-1689	CD	denotes	600
T12711	1690-1695	NN	denotes	µg/ml
T12712	1696-1700	NN	denotes	G418
T12713	1701-1708	NN	denotes	sulfate
T12714	1710-1720	JJ	denotes	Homologous
T12715	1721-1734	NN	denotes	recombination
T12716	1735-1737	IN	denotes	in
T12717	1738-1752	JJ	denotes	G418-resistant
T12718	1753-1759	NN	denotes	clones
T12719	1760-1763	VB	denotes	was
T12720	1764-1772	VB	denotes	detected
T12721	1773-1775	IN	denotes	by
T12722	1776-1779	NN	denotes	PCR
T12723	1780-1782	IN	denotes	of
T12724	1783-1790	JJ	denotes	genomic
T12725	1791-1794	NN	denotes	DNA
T12726	1794-1795	-COMMA-	denotes	,
T12727	1796-1798	IN	denotes	as
T12728	1799-1808	VB	denotes	described
T12729	1809-1810	-LRB-	denotes	[
T12730	1810-1811	CD	denotes	7
T12731	1811-1812	-RRB-	denotes	]
T12732	1814-1829	NN	denotes	Oligonucleotide
T12733	1830-1837	NN	denotes	primers
T12734	1838-1842	VB	denotes	used
T12735	1843-1846	IN	denotes	for
T12736	1847-1856	NN	denotes	screening
T12737	1857-1861	VB	denotes	were
T12738	1862-1894	JJ	denotes	5′-cttcctgatctcagctttaccgtcac-3′
T12739	1895-1896	-LRB-	denotes	(
T12740	1896-1906	JJ	denotes	homologous
T12741	1907-1909	TO	denotes	to
T12742	1910-1917	JJ	denotes	genomic
T12743	1918-1926	NN	denotes	sequence
T12744	1926-1927	-COLON-	denotes	;
T12745	1928-1939	JJ	denotes	approximate
T12746	1940-1948	NN	denotes	position
T12747	1949-1954	VB	denotes	noted
T12748	1955-1957	IN	denotes	in
T12749	1958-1962	NNP	denotes	Fig.
T12750	1963-1964	CD	denotes	1
T12751	1964-1965	-RRB-	denotes	)
T12752	1966-1969	CC	denotes	and
T12753	1970-1997	JJ	denotes	5′-caatccatcttgttcagccat-3′
T12754	1998-1999	-LRB-	denotes	(
T12755	1999-2009	JJ	denotes	homologous
T12756	2010-2012	TO	denotes	to
T12757	2013-2021	NN	denotes	sequence
T12758	2022-2024	IN	denotes	in
T12759	2025-2029	NN	denotes	NeoR
T12760	2029-2030	-RRB-	denotes	)
T12761	2032-2038	NN	denotes	Clones
T12762	2039-2041	IN	denotes	in
T12763	2042-2047	WDT	denotes	which
T12764	2048-2051	CD	denotes	one
T12765	2052-2056	NN	denotes	copy
T12766	2057-2059	IN	denotes	of
T12767	2060-2065	NN	denotes	Rnf31
T12768	2066-2069	VB	denotes	had
T12769	2070-2074	VB	denotes	been
T12770	2075-2084	VB	denotes	disrupted
T12771	2085-2089	VB	denotes	were
T12772	2090-2101	RB	denotes	transiently
T12773	2102-2113	VB	denotes	transfected
T12774	2114-2118	IN	denotes	with
T12775	2119-2121	DT	denotes	an
T12776	2122-2132	NN	denotes	expression
T12777	2133-2140	NN	denotes	plasmid
T12778	2141-2149	VB	denotes	encoding
T12779	2150-2153	NNP	denotes	Cre
T12780	2153-2154	-COMMA-	denotes	,
T12781	2155-2157	IN	denotes	in
T12782	2158-2163	NN	denotes	order
T12783	2164-2166	TO	denotes	to
T12784	2167-2173	VB	denotes	remove
T12785	2174-2178	NN	denotes	NeoR
T12786	2180-2194	JJ	denotes	G418-sensitive
T12787	2195-2204	NN	denotes	subclones
T12788	2205-2209	VB	denotes	were
T12789	2210-2219	VB	denotes	subjected
T12790	2220-2222	TO	denotes	to
T12791	2223-2224	DT	denotes	a
T12792	2225-2231	JJ	denotes	second
T12793	2232-2237	NN	denotes	round
T12794	2238-2240	IN	denotes	of
T12795	2241-2250	VB	denotes	targeting
T12796	2251-2253	TO	denotes	to
T12797	2254-2261	VB	denotes	disrupt
T12798	2262-2265	DT	denotes	the
T12799	2266-2275	VB	denotes	remaining
T12800	2276-2280	NN	denotes	copy
T12801	2281-2283	IN	denotes	of
T12802	2284-2289	NN	denotes	Rnf31
T12803	2291-2305	JJ	denotes	G418-resistant
T12804	2306-2312	NN	denotes	clones
T12805	2313-2317	VB	denotes	were
T12806	2318-2324	VB	denotes	tested
T12807	2325-2328	IN	denotes	for
T12808	2329-2339	JJ	denotes	homologous
T12809	2340-2353	NN	denotes	recombination
T12810	2354-2356	IN	denotes	by
T12811	2357-2360	NN	denotes	PCR
T12812	2361-2364	CC	denotes	and
T12813	2365-2369	NN	denotes	HOIP
T12814	2370-2377	NN	denotes	protein
T12815	2378-2388	NN	denotes	expression
T12816	2389-2391	IN	denotes	by
T12817	2392-2399	JJ	denotes	Western
T12818	2400-2404	NN	denotes	blot
T13106	2407-2417	JJ	denotes	Retroviral
T13107	2418-2430	NN	denotes	transduction
T13108	2431-2432	DT	denotes	A
T13109	2433-2443	JJ	denotes	retroviral
T13110	2444-2450	NN	denotes	vector
T13111	2451-2452	-LRB-	denotes	(
T13112	2452-2456	NN	denotes	pMIP
T13113	2456-2457	-RRB-	denotes	)
T13114	2458-2461	VB	denotes	was
T13115	2462-2466	VB	denotes	used
T13116	2467-2470	IN	denotes	for
T13117	2471-2474	DT	denotes	the
T13118	2475-2481	JJ	denotes	stable
T13119	2482-2494	NN	denotes	transduction
T13120	2495-2497	IN	denotes	of
T13121	2498-2503	NN	denotes	cells
T13122	2504-2508	IN	denotes	with
T13123	2509-2513	NN	denotes	HOIP
T13124	2514-2517	CC	denotes	and
T13125	2518-2522	NN	denotes	IKKγ
T13126	2523-2527	NN	denotes	cDNA
T13127	2528-2539	NN	denotes	constructs.
T13128	2540-2544	NN	denotes	pMIP
T13129	2545-2548	VB	denotes	was
T13130	2549-2560	VB	denotes	constructed
T13131	2561-2563	IN	denotes	by
T13132	2564-2573	VB	denotes	replacing
T13133	2574-2577	DT	denotes	the
T13134	2578-2582	NN	denotes	IRES
T13135	2583-2586	CC	denotes	and
T13136	2587-2590	NN	denotes	GFP
T13137	2591-2599	VB	denotes	encoding
T13138	2600-2609	NN	denotes	sequences
T13139	2610-2612	IN	denotes	in
T13140	2613-2617	NN	denotes	pMIG
T13141	2618-2619	-LRB-	denotes	[
T13142	2619-2621	CD	denotes	31
T13143	2621-2622	-RRB-	denotes	]
T13144	2623-2627	IN	denotes	with
T13145	2628-2631	DT	denotes	the
T13146	2632-2636	NN	denotes	IRES
T13147	2637-2640	CC	denotes	and
T13148	2641-2650	NN	denotes	puromycin
T13149	2651-2661	NN	denotes	resistance
T13150	2662-2666	NN	denotes	gene
T13151	2667-2671	IN	denotes	from
T13152	2672-2682	NN	denotes	pIRESpuro2
T13153	2683-2684	-LRB-	denotes	(
T13154	2684-2692	NN	denotes	Clontech
T13155	2692-2693	-RRB-	denotes	)
T13156	2695-2706	JJ	denotes	FLAG-tagged
T13157	2707-2712	NN	denotes	mouse
T13158	2713-2717	NN	denotes	HOIP
T13159	2718-2722	NN	denotes	cDNA
T13160	2723-2726	VB	denotes	was
T13161	2727-2735	VB	denotes	inserted
T13162	2736-2740	IN	denotes	into
T13163	2741-2744	DT	denotes	the
T13164	2745-2753	JJ	denotes	multiple
T13165	2754-2761	NN	denotes	cloning
T13166	2762-2766	NN	denotes	site
T13167	2767-2769	IN	denotes	of
T13168	2770-2774	NN	denotes	pMIP
T13169	2776-2777	DT	denotes	A
T13170	2778-2782	NN	denotes	pMIP
T13171	2783-2792	NN	denotes	construct
T13172	2793-2801	VB	denotes	encoding
T13173	2802-2807	NN	denotes	mouse
T13174	2808-2812	NN	denotes	IKKγ
T13175	2813-2817	IN	denotes	with
T13176	2818-2820	DT	denotes	an
T13177	2821-2826	JJ	denotes	amino
T13178	2827-2835	JJ	denotes	terminal
T13179	2836-2841	NN	denotes	birch
T13180	2842-2850	NN	denotes	profilin
T13181	2851-2858	NN	denotes	peptide
T13182	2859-2860	-LRB-	denotes	(
T13183	2860-2862	NN	denotes	BP
T13184	2862-2863	-RRB-	denotes	)
T13185	2863-2867	NN	denotes	-tag
T13186	2868-2871	VB	denotes	was
T13187	2872-2876	RB	denotes	also
T13188	2877-2885	VB	denotes	prepared
T13189	2887-2897	JJ	denotes	Retroviral
T13190	2898-2907	NN	denotes	particles
T13191	2908-2912	VB	denotes	were
T13192	2913-2922	VB	denotes	generated
T13193	2923-2925	IN	denotes	by
T13194	2926-2935	JJ	denotes	transient
T13195	2936-2948	NN	denotes	transfection
T13196	2949-2951	IN	denotes	of
T13197	2952-2956	NN	denotes	293T
T13198	2957-2962	NN	denotes	cells
T13199	2963-2967	IN	denotes	with
T13200	2968-2972	NN	denotes	pMIP
T13201	2972-2973	-COMMA-	denotes	,
T13202	2974-2983	NN	denotes	pMIP-HOIP
T13203	2983-2984	-COMMA-	denotes	,
T13204	2985-2987	CC	denotes	or
T13205	2988-2997	NN	denotes	pMIP-IKKγ
T13206	2998-3001	CC	denotes	and
T13207	3002-3005	DT	denotes	the
T13208	3006-3015	NN	denotes	packaging
T13209	3016-3022	NN	denotes	vector
T13210	3023-3030	NN	denotes	pCL-Eco
T13211	3031-3032	-LRB-	denotes	[
T13212	3032-3034	CD	denotes	32
T13213	3034-3035	-RRB-	denotes	]
T13214	3037-3040	CD	denotes	Two
T13215	3041-3045	NN	denotes	days
T13216	3046-3051	IN	denotes	after
T13217	3052-3064	NN	denotes	transfection
T13218	3064-3065	-COMMA-	denotes	,
T13219	3066-3073	NN	denotes	culture
T13220	3074-3086	NN	denotes	supernatants
T13221	3087-3091	VB	denotes	were
T13222	3092-3101	VB	denotes	harvested
T13223	3102-3105	CC	denotes	and
T13224	3106-3114	VB	denotes	filtered
T13225	3116-3122	NN	denotes	A20.2J
T13226	3123-3125	CC	denotes	or
T13227	3126-3140	JJ	denotes	HOIP-deficient
T13228	3141-3146	NN	denotes	cells
T13229	3147-3151	VB	denotes	were
T13230	3152-3157	VB	denotes	added
T13231	3158-3160	TO	denotes	to
T13232	3161-3168	JJ	denotes	24-well
T13233	3169-3175	NN	denotes	plates
T13234	3176-3177	-LRB-	denotes	(
T13235	3177-3184	CD	denotes	2.5×105
T13236	3185-3195	NN	denotes	cells/well
T13237	3195-3196	-RRB-	denotes	)
T13238	3197-3201	IN	denotes	with
T13239	3202-3203	CD	denotes	1
T13240	3204-3206	NN	denotes	ml
T13241	3207-3223	JJ	denotes	virus-containing
T13242	3224-3235	NN	denotes	supernatant
T13243	3236-3239	CC	denotes	and
T13244	3240-3241	CD	denotes	8
T13245	3242-3247	NN	denotes	µg/ml
T13246	3248-3257	NN	denotes	polybrene
T13247	3259-3265	NN	denotes	Plates
T13248	3266-3270	VB	denotes	were
T13249	3271-3282	VB	denotes	centrifuged
T13250	3283-3285	IN	denotes	at
T13251	3286-3289	CD	denotes	500
T13252	3290-3291	SYM	denotes	x
T13253	3292-3293	NN	denotes	g
T13254	3294-3297	IN	denotes	for
T13255	3298-3299	CD	denotes	2
T13256	3300-3303	NN	denotes	hrs
T13257	3304-3306	IN	denotes	at
T13258	3307-3311	NN	denotes	room
T13259	3312-3323	NN	denotes	temperature
T13260	3325-3330	NN	denotes	Cells
T13261	3331-3335	VB	denotes	were
T13262	3336-3344	VB	denotes	cultured
T13263	3345-3347	IN	denotes	in
T13264	3348-3349	NN	denotes	B
T13265	3350-3354	NN	denotes	cell
T13266	3355-3362	NN	denotes	culture
T13267	3363-3369	NN	denotes	medium
T13268	3370-3373	IN	denotes	for
T13269	3374-3376	CD	denotes	48
T13270	3377-3380	NN	denotes	hrs
T13271	3380-3381	-COMMA-	denotes	,
T13272	3382-3387	IN	denotes	after
T13273	3388-3393	WDT	denotes	which
T13274	3394-3397	DT	denotes	the
T13275	3398-3408	VB	denotes	transduced
T13276	3409-3414	NN	denotes	cells
T13277	3415-3419	VB	denotes	were
T13278	3420-3428	VB	denotes	selected
T13279	3429-3431	IN	denotes	in
T13280	3432-3433	CD	denotes	3
T13281	3434-3439	NN	denotes	µg/ml
T13282	3440-3449	NN	denotes	puromycin
T13427	3452-3456	NN	denotes	CD80
T13428	3457-3469	NN	denotes	upregulation
T13429	3470-3473	CC	denotes	and
T13430	3474-3478	NN	denotes	flow
T13431	3479-3488	NN	denotes	cytometry
T13432	3489-3494	NN	denotes	Cells
T13433	3495-3496	-LRB-	denotes	(
T13434	3496-3501	CD	denotes	5×104
T13435	3502-3504	IN	denotes	in
T13436	3505-3506	CD	denotes	2
T13437	3507-3509	NN	denotes	ml
T13438	3509-3510	-RRB-	denotes	)
T13439	3511-3515	VB	denotes	were
T13440	3516-3524	VB	denotes	cultured
T13441	3525-3527	IN	denotes	in
T13442	3528-3535	JJ	denotes	24-well
T13443	3536-3542	NN	denotes	plates
T13444	3543-3546	IN	denotes	for
T13445	3547-3548	CD	denotes	3
T13446	3549-3553	NN	denotes	days
T13447	3554-3558	IN	denotes	with
T13448	3559-3560	CD	denotes	5
T13449	3561-3566	NN	denotes	µg/ml
T13450	3567-3576	NN	denotes	anti-CD40
T13451	3577-3579	CC	denotes	or
T13452	3580-3582	DT	denotes	an
T13453	3583-3590	NN	denotes	isotype
T13454	3591-3598	NN	denotes	control
T13455	3599-3607	NN	denotes	antibody
T13456	3609-3614	NN	denotes	Cells
T13457	3615-3619	VB	denotes	were
T13458	3620-3627	VB	denotes	stained
T13459	3628-3631	IN	denotes	for
T13460	3632-3636	NN	denotes	flow
T13461	3637-3646	NN	denotes	cytometry
T13462	3647-3651	IN	denotes	with
T13463	3652-3666	NN	denotes	FITC-anti-CD80
T13464	3667-3669	CC	denotes	or
T13465	3670-3672	DT	denotes	an
T13466	3673-3684	JJ	denotes	appropriate
T13467	3685-3692	NN	denotes	control
T13468	3693-3701	NN	denotes	antibody
T13469	3702-3704	RB	denotes	as
T13470	3705-3714	VB	denotes	described
T13471	3715-3716	-LRB-	denotes	[
T13472	3716-3718	CD	denotes	28
T13473	3718-3719	-RRB-	denotes	]
T13474	3721-3725	NN	denotes	Data
T13475	3726-3734	VB	denotes	acquired
T13476	3735-3739	IN	denotes	with
T13477	3740-3741	DT	denotes	a
T13478	3742-3749	NN	denotes	FACScan
T13479	3750-3754	NN	denotes	flow
T13480	3755-3764	NN	denotes	cytometer
T13481	3765-3766	-LRB-	denotes	(
T13482	3766-3768	NN	denotes	BD
T13483	3769-3780	NNP	denotes	Biosciences
T13484	3780-3781	-RRB-	denotes	)
T13485	3782-3786	VB	denotes	were
T13486	3787-3795	VB	denotes	analyzed
T13487	3796-3800	IN	denotes	with
T13488	3801-3807	NNP	denotes	WinMDI
T13489	3808-3811	CD	denotes	2.8
T13490	3812-3813	-LRB-	denotes	(
T13491	3813-3820	NNP	denotes	Scripps
T13492	3821-3829	NNP	denotes	Research
T13493	3830-3839	NNP	denotes	Institute
T13494	3839-3840	-COMMA-	denotes	,
T13495	3841-3844	NNP	denotes	San
T13496	3845-3850	NNP	denotes	Diego
T13497	3850-3851	-COMMA-	denotes	,
T13498	3852-3854	NNP	denotes	CA
T13499	3854-3855	-RRB-	denotes	)
T13684	3858-3861	NN	denotes	GLε
T13685	3862-3872	NN	denotes	transcript
T13686	3873-3878	NN	denotes	assay
T13687	3879-3882	DT	denotes	The
T13688	3883-3897	JJ	denotes	CD40-simulated
T13689	3898-3908	NN	denotes	activation
T13690	3909-3911	IN	denotes	of
T13691	3912-3915	NN	denotes	GLε
T13692	3916-3929	NN	denotes	transcription
T13693	3930-3933	VB	denotes	was
T13694	3934-3943	VB	denotes	evaluated
T13695	3944-3946	IN	denotes	as
T13696	3947-3957	RB	denotes	previously
T13697	3958-3966	VB	denotes	reported
T13698	3967-3968	-LRB-	denotes	[
T13699	3968-3970	CD	denotes	33
T13700	3970-3971	-RRB-	denotes	]
T13701	3973-3980	NNP	denotes	Briefly
T13702	3980-3981	-COMMA-	denotes	,
T13703	3982-3987	CD	denotes	1×106
T13704	3988-3993	NN	denotes	cells
T13705	3994-3998	VB	denotes	were
T13706	3999-4009	VB	denotes	stimulated
T13707	4010-4019	RB	denotes	overnight
T13708	4020-4024	IN	denotes	with
T13709	4025-4034	JJ	denotes	anti-CD40
T13710	4035-4043	NN	denotes	antibody
T13711	4044-4045	-LRB-	denotes	(
T13712	4045-4047	CD	denotes	10
T13713	4048-4053	NN	denotes	µg/ml
T13714	4053-4054	-RRB-	denotes	)
T13715	4055-4057	CC	denotes	or
T13716	4058-4060	DT	denotes	an
T13717	4061-4068	NN	denotes	isotype
T13718	4069-4076	NN	denotes	control
T13719	4077-4085	NN	denotes	antibody
T13720	4085-4086	-COMMA-	denotes	,
T13721	4087-4091	IN	denotes	with
T13722	4092-4094	CC	denotes	or
T13723	4095-4102	IN	denotes	without
T13724	4103-4106	CD	denotes	500
T13725	4107-4111	NN	denotes	U/ml
T13726	4112-4117	NN	denotes	mouse
T13727	4118-4122	NN	denotes	IL-4
T13728	4123-4124	-LRB-	denotes	(
T13729	4124-4126	NN	denotes	BD
T13730	4127-4138	NNP	denotes	Biosciences
T13731	4138-4139	-RRB-	denotes	)
T13732	4141-4144	NN	denotes	RNA
T13733	4145-4148	VB	denotes	was
T13734	4149-4157	VB	denotes	isolated
T13735	4158-4163	VB	denotes	using
T13736	4164-4170	NN	denotes	Trizol
T13737	4171-4172	-LRB-	denotes	(
T13738	4172-4182	NN	denotes	Invitrogen
T13739	4182-4183	-RRB-	denotes	)
T13740	4183-4184	-COMMA-	denotes	,
T13741	4185-4188	CC	denotes	and
T13742	4189-4208	JJ	denotes	reverse-transcribed
T13743	4209-4210	-LRB-	denotes	(
T13744	4210-4221	NN	denotes	Superscript
T13745	4222-4225	CD	denotes	III
T13746	4226-4229	NN	denotes	kit
T13747	4229-4230	-COMMA-	denotes	,
T13748	4231-4241	NN	denotes	Invitrogen
T13749	4241-4242	-RRB-	denotes	)
T13750	4244-4256	JJ	denotes	Quantitative
T13751	4257-4260	NN	denotes	PCR
T13752	4261-4264	IN	denotes	for
T13753	4265-4268	NN	denotes	GLε
T13754	4269-4272	CC	denotes	and
T13755	4273-4278	NN	denotes	Hprt1
T13756	4279-4282	VB	denotes	was
T13757	4283-4292	VB	denotes	performed
T13758	4293-4298	VB	denotes	using
T13759	4299-4303	NN	denotes	SYBR
T13760	4304-4309	NN	denotes	GREEN
T13761	4310-4316	NN	denotes	master
T13762	4317-4320	NN	denotes	mix
T13763	4321-4322	-LRB-	denotes	(
T13764	4322-4329	NNP	denotes	Applied
T13765	4330-4340	NNP	denotes	Biosystems
T13766	4340-4341	-RRB-	denotes	)
T13767	4341-4342	-COMMA-	denotes	,
T13768	4343-4346	CC	denotes	and
T13769	4347-4349	DT	denotes	an
T13770	4350-4357	NNP	denotes	Applied
T13771	4358-4368	NNP	denotes	Biosystems
T13772	4369-4375	NNP	denotes	7900HT
T13773	4376-4380	NNP	denotes	Fast
T13774	4381-4390	NNP	denotes	Real-Time
T13775	4391-4394	NN	denotes	PCR
T13776	4395-4405	NN	denotes	instrument
T13777	4407-4417	NN	denotes	Expression
T13778	4418-4420	IN	denotes	of
T13779	4421-4424	NN	denotes	GLε
T13780	4425-4427	IN	denotes	in
T13781	4428-4432	DT	denotes	each
T13782	4433-4439	NN	denotes	sample
T13783	4440-4443	VB	denotes	was
T13784	4444-4454	VB	denotes	normalized
T13785	4455-4457	TO	denotes	to
T13786	4458-4461	DT	denotes	the
T13787	4462-4472	NN	denotes	expression
T13788	4473-4475	IN	denotes	of
T13789	4476-4481	NN	denotes	Hprt1
T14056	4484-4489	NN	denotes	NF-κB
T14057	4490-4493	CC	denotes	and
T14058	4494-4497	NN	denotes	JNK
T14059	4498-4508	NN	denotes	activation
T14060	4509-4515	NN	denotes	assays
T14061	4516-4519	IN	denotes	For
T14062	4520-4530	NN	denotes	activation
T14063	4531-4533	IN	denotes	of
T14064	4534-4538	NN	denotes	CD40
T14065	4539-4548	NN	denotes	signaling
T14066	4548-4549	-COMMA-	denotes	,
T14067	4550-4555	CD	denotes	5×104
T14068	4556-4559	NN	denotes	HI5
T14069	4560-4566	NN	denotes	insect
T14070	4567-4572	NN	denotes	cells
T14071	4573-4575	CC	denotes	or
T14072	4576-4579	NN	denotes	HI5
T14073	4580-4586	NN	denotes	insect
T14074	4587-4592	NN	denotes	cells
T14075	4593-4603	VB	denotes	expressing
T14076	4604-4609	NN	denotes	mouse
T14077	4610-4615	NN	denotes	CD154
T14078	4616-4617	-LRB-	denotes	(
T14079	4617-4621	NN	denotes	CD40
T14080	4622-4628	NN	denotes	ligand
T14081	4628-4629	-RRB-	denotes	)
T14082	4630-4634	VB	denotes	were
T14083	4635-4640	VB	denotes	added
T14084	4641-4643	TO	denotes	to
T14085	4644-4649	CD	denotes	1×106
T14086	4650-4651	NN	denotes	B
T14087	4652-4657	NN	denotes	cells
T14088	4659-4664	NN	denotes	Cells
T14089	4665-4669	VB	denotes	were
T14090	4670-4681	VB	denotes	centrifuged
T14091	4682-4685	IN	denotes	for
T14092	4686-4687	CD	denotes	1
T14093	4688-4694	NN	denotes	minute
T14094	4695-4697	IN	denotes	at
T14095	4698-4701	CD	denotes	400
T14096	4702-4703	SYM	denotes	x
T14097	4704-4705	NN	denotes	g
T14098	4706-4707	-LRB-	denotes	(
T14099	4707-4709	TO	denotes	to
T14100	4710-4717	VB	denotes	promote
T14101	4718-4725	NN	denotes	contact
T14102	4726-4733	IN	denotes	between
T14103	4734-4740	NN	denotes	ligand
T14104	4741-4746	NN	denotes	cells
T14105	4747-4750	CC	denotes	and
T14106	4751-4752	NN	denotes	B
T14107	4753-4758	NN	denotes	cells
T14108	4758-4759	-RRB-	denotes	)
T14109	4760-4764	RB	denotes	then
T14110	4765-4774	VB	denotes	incubated
T14111	4775-4777	IN	denotes	at
T14112	4778-4782	NN	denotes	37°C
T14113	4783-4786	IN	denotes	for
T14114	4787-4792	NN	denotes	times
T14115	4793-4802	VB	denotes	indicated
T14116	4803-4805	IN	denotes	in
T14117	4806-4810	NNP	denotes	Fig.
T14118	4811-4812	CD	denotes	4
T14119	4814-4823	VB	denotes	Following
T14120	4824-4835	NN	denotes	stimulation
T14121	4835-4836	-COMMA-	denotes	,
T14122	4837-4842	NN	denotes	cells
T14123	4843-4847	VB	denotes	were
T14124	4848-4855	VB	denotes	chilled
T14125	4856-4858	IN	denotes	on
T14126	4859-4862	NN	denotes	ice
T14127	4863-4866	IN	denotes	for
T14128	4867-4868	CD	denotes	2
T14129	4869-4876	NN	denotes	minutes
T14130	4878-4882	NN	denotes	Cell
T14131	4883-4890	NN	denotes	pellets
T14132	4891-4895	VB	denotes	were
T14133	4896-4905	VB	denotes	dissolved
T14134	4906-4908	IN	denotes	in
T14135	4909-4911	NN	denotes	2X
T14136	4912-4920	NN	denotes	SDS-PAGE
T14137	4921-4928	VB	denotes	loading
T14138	4929-4935	NN	denotes	buffer
T14139	4935-4936	-COMMA-	denotes	,
T14140	4937-4946	VB	denotes	sonicated
T14141	4946-4947	-COMMA-	denotes	,
T14142	4948-4951	CC	denotes	and
T14143	4952-4958	VB	denotes	heated
T14144	4959-4962	IN	denotes	for
T14145	4963-4964	CD	denotes	5
T14146	4965-4972	NN	denotes	minutes
T14147	4973-4975	IN	denotes	at
T14148	4976-4981	NNP	denotes	95°C.
T14149	4982-4987	NNP	denotes	Total
T14150	4988-4992	NN	denotes	cell
T14151	4993-5000	NN	denotes	lysates
T14152	5001-5002	-LRB-	denotes	(
T14153	5002-5007	CD	denotes	1×105
T14154	5008-5012	NN	denotes	cell
T14155	5013-5024	NN	denotes	equivalents
T14156	5025-5028	IN	denotes	per
T14157	5029-5033	NN	denotes	lane
T14158	5033-5034	-RRB-	denotes	)
T14159	5035-5039	VB	denotes	were
T14160	5040-5052	VB	denotes	fractionated
T14161	5053-5055	IN	denotes	by
T14162	5056-5064	NN	denotes	SDS-PAGE
T14163	5065-5068	CC	denotes	and
T14164	5069-5076	VB	denotes	blotted
T14165	5077-5079	TO	denotes	to
T14166	5080-5094	NN	denotes	polyvinylidine
T14167	5095-5103	NN	denotes	fluoride
T14168	5104-5105	-LRB-	denotes	(
T14169	5105-5109	NN	denotes	PVDF
T14170	5109-5110	-RRB-	denotes	)
T14171	5111-5120	NN	denotes	membranes
T14172	5122-5131	NN	denotes	Membranes
T14173	5132-5136	VB	denotes	were
T14174	5137-5143	VB	denotes	probed
T14175	5144-5148	IN	denotes	with
T14176	5149-5159	NN	denotes	antibodies
T14177	5160-5162	IN	denotes	as
T14178	5163-5172	VB	denotes	indicated
T14179	5173-5175	IN	denotes	in
T14180	5176-5180	NNP	denotes	Fig.
T14181	5181-5182	CD	denotes	4
T14182	5184-5200	JJ	denotes	Chemiluminescent
T14183	5201-5210	NN	denotes	detection
T14184	5211-5212	-LRB-	denotes	(
T14185	5212-5218	NNP	denotes	Pierce
T14186	5219-5232	NNP	denotes	Biotechnology
T14187	5232-5233	-RRB-	denotes	)
T14188	5234-5237	VB	denotes	was
T14189	5238-5242	VB	denotes	used
T14190	5243-5246	IN	denotes	for
T14191	5247-5250	DT	denotes	the
T14192	5251-5264	NN	denotes	visualization
T14193	5265-5267	IN	denotes	of
T14194	5268-5273	NN	denotes	bands
T14195	5274-5276	IN	denotes	on
T14196	5277-5284	JJ	denotes	Western
T14197	5285-5290	NN	denotes	blots
T14198	5292-5298	NN	denotes	Images
T14199	5299-5301	IN	denotes	of
T14200	5302-5307	NN	denotes	blots
T14201	5308-5312	VB	denotes	were
T14202	5313-5321	VB	denotes	recorded
T14203	5322-5326	IN	denotes	with
T14204	5327-5328	DT	denotes	a
T14205	5329-5338	JJ	denotes	low-light
T14206	5339-5346	NN	denotes	imaging
T14207	5347-5353	NN	denotes	system
T14208	5354-5355	-LRB-	denotes	(
T14209	5355-5362	NN	denotes	LAS4000
T14210	5362-5363	-COMMA-	denotes	,
T14211	5364-5368	NNP	denotes	Fuji
T14212	5369-5376	NNP	denotes	Medical
T14213	5377-5384	NNP	denotes	Systems
T14214	5384-5385	-RRB-	denotes	)
T14215	5386-5389	CC	denotes	and
T14216	5390-5392	IN	denotes	on
T14217	5393-5398	NN	denotes	X-ray
T14218	5399-5403	NN	denotes	film
T14502	5406-5426	NN	denotes	Immunoprecipitations
T14503	5427-5446	NN	denotes	Immunoprecipitation
T14504	5447-5449	IN	denotes	of
T14505	5450-5454	NN	denotes	CD40
T14506	5455-5458	VB	denotes	was
T14507	5459-5468	VB	denotes	performed
T14508	5469-5471	IN	denotes	by
T14509	5472-5481	VB	denotes	activated
T14510	5482-5490	NN	denotes	receptor
T14511	5491-5498	NN	denotes	capture
T14512	5498-5499	-COMMA-	denotes	,
T14513	5500-5502	IN	denotes	as
T14514	5503-5513	RB	denotes	previously
T14515	5514-5523	VB	denotes	described
T14516	5524-5525	-LRB-	denotes	[
T14517	5525-5526	CD	denotes	6
T14518	5526-5527	-RRB-	denotes	]
T14519	5529-5536	NNP	denotes	Briefly
T14520	5536-5537	-COMMA-	denotes	,
T14521	5538-5543	CD	denotes	3×107
T14522	5544-5549	NN	denotes	cells
T14523	5550-5551	-LRB-	denotes	(
T14524	5551-5557	NN	denotes	A20.2J
T14525	5558-5561	CC	denotes	and
T14526	5562-5573	NN	denotes	derivatives
T14527	5573-5574	-RRB-	denotes	)
T14528	5575-5579	VB	denotes	were
T14529	5580-5589	VB	denotes	incubated
T14530	5590-5593	IN	denotes	for
T14531	5594-5596	CD	denotes	60
T14532	5597-5604	NN	denotes	minutes
T14533	5605-5607	IN	denotes	at
T14534	5608-5612	NN	denotes	room
T14535	5613-5624	NN	denotes	temperature
T14536	5625-5629	IN	denotes	with
T14537	5630-5632	CD	denotes	10
T14538	5633-5635	NN	denotes	µl
T14539	5636-5644	JJ	denotes	magnetic
T14540	5645-5652	NN	denotes	protein
T14541	5653-5654	NN	denotes	G
T14542	5655-5660	NN	denotes	beads
T14543	5661-5662	-LRB-	denotes	(
T14544	5662-5667	JJ	denotes	Dynal
T14545	5667-5668	-RRB-	denotes	)
T14546	5669-5679	JJ	denotes	pre-coated
T14547	5680-5684	IN	denotes	with
T14548	5685-5687	CD	denotes	10
T14549	5688-5690	NN	denotes	µg
T14550	5691-5695	NN	denotes	goat
T14551	5696-5704	JJ	denotes	anti-rat
T14552	5705-5708	NN	denotes	IgG
T14553	5709-5710	-LRB-	denotes	(
T14554	5710-5717	NN	denotes	Jackson
T14555	5717-5718	-RRB-	denotes	)
T14556	5718-5719	-COMMA-	denotes	,
T14557	5720-5723	CC	denotes	and
T14558	5724-5726	CD	denotes	10
T14559	5727-5729	NN	denotes	µg
T14560	5730-5739	NN	denotes	anti-CD40
T14561	5740-5741	-LRB-	denotes	(
T14562	5741-5745	NN	denotes	1C10
T14563	5745-5746	-RRB-	denotes	)
T14564	5747-5749	CC	denotes	or
T14565	5750-5752	DT	denotes	an
T14566	5753-5760	NN	denotes	isotype
T14567	5761-5768	NN	denotes	control
T14568	5769-5777	NN	denotes	antibody
T14569	5778-5779	-LRB-	denotes	(
T14570	5779-5784	NN	denotes	mAb72
T14571	5784-5785	-RRB-	denotes	)
T14572	5787-5798	NN	denotes	Cells/beads
T14573	5799-5803	VB	denotes	were
T14574	5804-5808	RB	denotes	then
T14575	5809-5817	VB	denotes	pelleted
T14576	5818-5820	IN	denotes	by
T14577	5821-5835	NN	denotes	centrifugation
T14578	5836-5839	CC	denotes	and
T14579	5840-5845	VB	denotes	lysed
T14580	5846-5848	IN	denotes	in
T14581	5849-5855	NN	denotes	buffer
T14582	5856-5866	VB	denotes	containing
T14583	5867-5868	CD	denotes	1
T14584	5868-5869	NN	denotes	%
T14585	5870-5876	NN	denotes	Triton
T14586	5877-5881	NN	denotes	X100
T14587	5883-5888	NN	denotes	Beads
T14588	5889-5893	VB	denotes	were
T14589	5894-5900	VB	denotes	washed
T14590	5901-5905	IN	denotes	with
T14591	5906-5911	NN	denotes	lysis
T14592	5912-5918	NN	denotes	buffer
T14593	5919-5921	TO	denotes	to
T14594	5922-5928	VB	denotes	remove
T14595	5929-5936	JJ	denotes	unbound
T14596	5937-5945	NN	denotes	material
T14597	5947-5949	IN	denotes	In
T14598	5950-5954	DT	denotes	some
T14599	5955-5966	NN	denotes	experiments
T14600	5966-5967	-COMMA-	denotes	,
T14601	5968-5976	NN	denotes	material
T14602	5977-5987	VB	denotes	associated
T14603	5988-5992	IN	denotes	with
T14604	5993-5996	DT	denotes	the
T14605	5997-6002	NN	denotes	beads
T14606	6003-6006	VB	denotes	was
T14607	6007-6023	VB	denotes	dephosphorylated
T14608	6024-6028	IN	denotes	with
T14609	6029-6035	NN	denotes	lambda
T14610	6036-6047	NN	denotes	phosphatase
T14611	6048-6049	-LRB-	denotes	(
T14612	6049-6052	NNP	denotes	New
T14613	6053-6060	NNP	denotes	England
T14614	6061-6068	NNP	denotes	BioLabs
T14615	6068-6069	-RRB-	denotes	)
T14616	6070-6072	IN	denotes	as
T14617	6073-6076	IN	denotes	per
T14618	6077-6089	NN	denotes	manufacturer
T14619	6089-6091	POS	denotes	's
T14620	6092-6104	NN	denotes	instructions
T14621	6106-6111	NN	denotes	Beads
T14622	6112-6116	VB	denotes	were
T14623	6117-6128	VB	denotes	resuspended
T14624	6129-6131	IN	denotes	in
T14625	6132-6134	NN	denotes	2X
T14626	6135-6143	NN	denotes	SDS-PAGE
T14627	6144-6150	NN	denotes	sample
T14628	6151-6157	NN	denotes	buffer
T14629	6158-6161	CC	denotes	and
T14630	6162-6168	VB	denotes	heated
T14631	6169-6172	IN	denotes	for
T14632	6173-6174	CD	denotes	5
T14633	6175-6182	NN	denotes	minutes
T14634	6183-6185	IN	denotes	at
T14635	6186-6191	NNP	denotes	95°C.
T14636	6192-6200	NNP	denotes	Material
T14637	6201-6207	VB	denotes	eluted
T14638	6208-6212	IN	denotes	from
T14639	6213-6216	DT	denotes	the
T14640	6217-6222	NN	denotes	beads
T14641	6223-6226	VB	denotes	was
T14642	6227-6239	VB	denotes	fractionated
T14643	6240-6242	IN	denotes	by
T14644	6243-6251	NN	denotes	SDS-PAGE
T14645	6252-6255	CC	denotes	and
T14646	6256-6267	VB	denotes	transferred
T14647	6268-6270	TO	denotes	to
T14648	6271-6275	NN	denotes	PVDF
T14649	6276-6285	NN	denotes	membranes
T14650	6286-6289	IN	denotes	for
T14651	6290-6297	JJ	denotes	Western
T14652	6298-6306	NN	denotes	blotting
T14653	6308-6310	IN	denotes	In
T14654	6311-6315	DT	denotes	some
T14655	6316-6327	NN	denotes	experiments
T14656	6327-6328	-COMMA-	denotes	,
T14657	6329-6334	NN	denotes	cells
T14658	6335-6339	VB	denotes	were
T14659	6340-6348	VB	denotes	cultured
T14660	6349-6352	IN	denotes	for
T14661	6353-6354	CD	denotes	6
T14662	6355-6358	NN	denotes	hrs
T14663	6359-6363	IN	denotes	with
T14664	6364-6366	CD	denotes	25
T14665	6367-6369	NN	denotes	µM
T14666	6370-6389	JJ	denotes	antennapedia-linked
T14667	6390-6397	NN	denotes	SMAC-N7
T14668	6398-6405	NN	denotes	peptide
T14669	6406-6407	-LRB-	denotes	(
T14670	6407-6417	NN	denotes	Calbiochem
T14671	6417-6418	-RRB-	denotes	)
T14672	6419-6421	CC	denotes	or
T14673	6422-6424	DT	denotes	an
T14674	6425-6436	JJ	denotes	appropriate
T14675	6437-6443	NN	denotes	volume
T14676	6444-6446	IN	denotes	of
T14677	6447-6450	DT	denotes	the
T14678	6451-6458	NN	denotes	solvent
T14679	6459-6463	VB	denotes	used
T14680	6464-6467	IN	denotes	for
T14681	6468-6471	DT	denotes	the
T14682	6472-6479	NN	denotes	peptide
T14683	6480-6481	-LRB-	denotes	(
T14684	6481-6485	NN	denotes	DMSO
T14685	6485-6486	-RRB-	denotes	)
T14686	6488-6493	IN	denotes	After
T14687	6494-6504	NN	denotes	incubation
T14688	6504-6505	-COMMA-	denotes	,
T14689	6506-6511	NN	denotes	cells
T14690	6512-6513	-LRB-	denotes	(
T14691	6513-6515	IN	denotes	in
T14692	6516-6524	JJ	denotes	peptide-
T14693	6525-6527	CC	denotes	or
T14694	6528-6543	JJ	denotes	DMSO-containing
T14695	6544-6550	NN	denotes	medium
T14696	6550-6551	-RRB-	denotes	)
T14697	6552-6556	VB	denotes	were
T14698	6557-6567	VB	denotes	stimulated
T14699	6568-6572	IN	denotes	with
T14700	6573-6588	JJ	denotes	antibody-coated
T14701	6589-6594	NN	denotes	beads
T14702	6595-6597	IN	denotes	as
T14703	6598-6606	VB	denotes	outlined
T14704	6607-6612	IN	denotes	above
R9305	T11984	T11983	arg1Of	lines,Cell
R9306	T11990	T11985	arg1Of	A20.2J,The
R9307	T11990	T11986	arg1Of	A20.2J,mouse
R9308	T11990	T11987	arg1Of	A20.2J,B
R9309	T11990	T11988	arg1Of	A20.2J,cell
R9310	T11990	T11989	arg1Of	A20.2J,line
R9311	T11990	T11991	arg1Of	A20.2J,has
R9312	T11990	T11992	arg1Of	A20.2J,been
R9313	T11990	T11994	arg2Of	A20.2J,described
R9314	T11994	T11991	arg2Of	described,has
R9315	T11994	T11992	arg2Of	described,been
R9316	T11994	T11993	arg1Of	described,previously
R9317	T11994	T11995	arg1Of	described,[
R9318	T11994	T11998	arg1Of	described,","
R9319	T11994	T11999	arg1Of	described,[
R9320	T11996	T11995	arg2Of	26,[
R9321	T11997	T11995	arg3Of	],[
R9322	T12000	T11999	arg2Of	27,[
R9323	T12001	T11999	arg3Of	],[
R9324	T12002	T12003	arg1Of	Cells,were
R9325	T12002	T12004	arg2Of	Cells,grown
R9326	T12004	T12003	arg2Of	grown,were
R9327	T12004	T12005	arg1Of	grown,in
R9328	T12006	T12007	arg1Of	RPMI,1640
R9329	T12006	T12008	arg1Of	RPMI,","
R9330	T12008	T12012	arg1Of	",",","
R9331	T12009	T12010	arg1Of	10,%
R9332	T12011	T12008	arg2Of	FCS,","
R9333	T12011	T12009	arg1Of	FCS,10
R9334	T12012	T12016	arg1Of	",",","
R9335	T12015	T12012	arg2Of	2-ME,","
R9336	T12015	T12013	arg1Of	2-ME,10
R9337	T12015	T12014	arg1Of	2-ME,µM
R9338	T12016	T12021	arg1Of	",",and
R9339	T12017	T12018	arg1Of	2,mM
R9340	T12019	T12016	arg2Of	L-glutamine,","
R9341	T12019	T12017	arg1Of	L-glutamine,2
R9342	T12021	T12005	arg2Of	and,in
R9343	T12021	T12020	arg1Of	and,","
R9344	T12022	T12021	arg2Of	antibiotics,and
R9345	T12025	T12023	arg1Of	cells,HI-5
R9346	T12025	T12024	arg1Of	cells,insect
R9347	T12025	T12026	arg1Of	cells,(
R9348	T12025	T12029	arg1Of	cells,expressing
R9349	T12025	T12031	arg1Of	cells,have
R9350	T12025	T12032	arg1Of	cells,been
R9351	T12025	T12033	arg2Of	cells,described
R9352	T12027	T12026	arg2Of	Invitrogen,(
R9353	T12028	T12026	arg3Of	),(
R9354	T12030	T12029	arg2Of	CD154,expressing
R9355	T12033	T12031	arg2Of	described,have
R9356	T12033	T12032	arg2Of	described,been
R9357	T12033	T12034	arg1Of	described,[
R9358	T12035	T12034	arg2Of	28,[
R9359	T12036	T12034	arg3Of	],[
R9468	T12167	T12164	arg1Of	antibody,Mouse
R9469	T12167	T12165	arg1Of	antibody,anti-birch
R9470	T12167	T12166	arg1Of	antibody,profilin
R9471	T12167	T12168	arg1Of	antibody,(
R9472	T12167	T12174	arg1Of	antibody,","
R9473	T12169	T12168	arg2Of	4A6,(
R9474	T12169	T12170	arg1Of	4A6,[
R9475	T12171	T12170	arg2Of	29,[
R9476	T12172	T12170	arg3Of	],[
R9477	T12173	T12168	arg3Of	),(
R9478	T12174	T12184	arg1Of	",",and
R9479	T12177	T12174	arg2Of	CD40,","
R9480	T12177	T12175	arg1Of	CD40,rat
R9481	T12177	T12176	arg1Of	CD40,anti-mouse
R9482	T12177	T12178	arg1Of	CD40,(
R9483	T12179	T12178	arg2Of	1C10,(
R9484	T12179	T12180	arg1Of	1C10,[
R9485	T12181	T12180	arg2Of	30,[
R9486	T12182	T12180	arg3Of	],[
R9487	T12183	T12178	arg3Of	),(
R9488	T12184	T12207	arg1Of	and,were
R9489	T12184	T12208	arg2Of	and,isolated
R9490	T12189	T12184	arg2Of	antibody,and
R9491	T12189	T12185	arg1Of	antibody,a
R9492	T12189	T12186	arg1Of	antibody,rat
R9493	T12189	T12187	arg1Of	antibody,isotype
R9494	T12189	T12188	arg1Of	antibody,control
R9495	T12189	T12190	arg1Of	antibody,(
R9496	T12191	T12190	arg2Of	mAb72,(
R9497	T12191	T12192	arg1Of	mAb72,","
R9498	T12191	T12197	arg1Of	mAb72,","
R9499	T12191	T12201	arg1Of	mAb72,","
R9500	T12196	T12192	arg2Of	Bank,","
R9501	T12196	T12193	arg1Of	Bank,Developmental
R9502	T12196	T12194	arg1Of	Bank,Studies
R9503	T12196	T12195	arg1Of	Bank,Hybridoma
R9504	T12198	T12197	arg2Of	University,","
R9505	T12198	T12199	arg1Of	University,of
R9506	T12200	T12199	arg2Of	Iowa,of
R9507	T12203	T12201	arg2Of	City,","
R9508	T12203	T12202	arg1Of	City,Iowa
R9509	T12203	T12204	arg1Of	City,","
R9510	T12205	T12204	arg2Of	IA,","
R9511	T12206	T12190	arg3Of	),(
R9512	T12208	T12207	arg2Of	isolated,were
R9513	T12208	T12209	arg1Of	isolated,from
R9514	T12211	T12209	arg2Of	supernatants,from
R9515	T12211	T12210	arg1Of	supernatants,hybridoma
R9516	T12214	T12212	arg1Of	CD80,FITC-labeled
R9517	T12214	T12213	arg1Of	CD80,anti-mouse
R9518	T12214	T12215	arg1Of	CD80,and
R9519	T12215	T12220	arg1Of	and,were
R9520	T12215	T12221	arg1Of	and,from
R9521	T12219	T12215	arg2Of	Ab,and
R9522	T12219	T12216	arg1Of	Ab,an
R9523	T12219	T12217	arg1Of	Ab,isotype
R9524	T12219	T12218	arg1Of	Ab,control
R9525	T12221	T12220	arg2Of	from,were
R9526	T12222	T12221	arg2Of	eBiosciences,from
R9527	T12225	T12223	arg1Of	Ab,Rabbit
R9528	T12225	T12224	arg1Of	Ab,anti-HOIP
R9529	T12225	T12226	arg1Of	Ab,[
R9530	T12225	T12229	arg1Of	Ab,was
R9531	T12227	T12226	arg2Of	6,[
R9532	T12228	T12226	arg3Of	],[
R9533	T12232	T12229	arg2Of	gift,was
R9534	T12232	T12230	arg1Of	gift,the
R9535	T12232	T12231	arg1Of	gift,kind
R9536	T12232	T12233	arg1Of	gift,of
R9537	T12236	T12233	arg2Of	Eipper,of
R9538	T12236	T12234	arg1Of	Eipper,Dr.
R9539	T12236	T12235	arg1Of	Eipper,Betty
R9540	T12236	T12237	arg1Of	Eipper,(
R9541	T12238	T12239	arg1Of	University,of
R9542	T12238	T12243	arg1Of	University,","
R9543	T12242	T12239	arg2Of	Center,of
R9544	T12242	T12240	arg1Of	Center,Connecticut
R9545	T12242	T12241	arg1Of	Center,Health
R9546	T12243	T12237	arg2Of	",",(
R9547	T12244	T12243	arg2Of	Farmington,","
R9548	T12244	T12245	arg1Of	Farmington,","
R9549	T12246	T12245	arg2Of	Connecticut,","
R9550	T12247	T12237	arg3Of	),(
R9551	T12250	T12248	arg1Of	IgG,Goat
R9552	T12250	T12249	arg1Of	IgG,anti-rat
R9553	T12250	T12252	arg1Of	IgG,and
R9554	T12252	T12251	arg1Of	and,","
R9555	T12252	T12256	arg1Of	and,were
R9556	T12252	T12257	arg1Of	and,from
R9557	T12255	T12252	arg2Of	Abs,and
R9558	T12255	T12253	arg1Of	Abs,HRP-labeled
R9559	T12255	T12254	arg1Of	Abs,secondary
R9560	T12256	T12261	arg1Of	were,","
R9561	T12257	T12256	arg2Of	from,were
R9562	T12260	T12257	arg2Of	Laboratories,from
R9563	T12260	T12258	arg1Of	Laboratories,Jackson
R9564	T12260	T12259	arg1Of	Laboratories,ImmunoResearch
R9565	T12265	T12262	arg1Of	antibodies,Inc.
R9566	T12265	T12263	arg1Of	antibodies,All
R9567	T12265	T12264	arg1Of	antibodies,other
R9568	T12265	T12266	arg2Of	antibodies,used
R9569	T12265	T12267	arg1Of	antibodies,were
R9570	T12265	T12268	arg2Of	antibodies,described
R9571	T12268	T12261	arg2Of	described,","
R9572	T12268	T12267	arg2Of	described,were
R9573	T12268	T12269	arg1Of	described,previously
R9574	T12268	T12270	arg1Of	described,[
R9575	T12271	T12270	arg2Of	6,[
R9576	T12272	T12270	arg3Of	],[
R9850	T12599	T12597	arg1Of	gene,Somatic
R9851	T12599	T12598	arg1Of	gene,cell
R9852	T12600	T12599	arg1Of	targeting,gene
R9853	T12602	T12601	arg1Of	generation,The
R9854	T12602	T12603	arg1Of	generation,of
R9855	T12602	T12606	arg1Of	generation,was
R9856	T12602	T12607	arg2Of	generation,accomplished
R9857	T12605	T12603	arg2Of	cells,of
R9858	T12605	T12604	arg1Of	cells,HOIP-deficient
R9859	T12607	T12606	arg2Of	accomplished,was
R9860	T12607	T12608	modOf	accomplished,using
R9861	T12607	T12618	arg1Of	accomplished,","
R9862	T12607	T12619	arg1Of	accomplished,[
R9863	T12612	T12608	arg2Of	approach,using
R9864	T12612	T12609	arg1Of	approach,a
R9865	T12612	T12610	arg1Of	approach,homologous
R9866	T12612	T12611	arg1Of	approach,recombination
R9867	T12612	T12613	arg2Of	approach,described
R9868	T12613	T12614	arg1Of	described,previously
R9869	T12613	T12615	arg1Of	described,[
R9870	T12616	T12615	arg2Of	7,[
R9871	T12617	T12615	arg3Of	],[
R9872	T12620	T12619	arg2Of	8,[
R9873	T12621	T12619	arg3Of	],[
R9874	T12622	T12623	arg1Of	Segments,of
R9875	T12622	T12636	arg1Of	Segments,were
R9876	T12622	T12637	arg2Of	Segments,amplified
R9877	T12626	T12623	arg2Of	sequence,of
R9878	T12626	T12624	arg1Of	sequence,Rnf31
R9879	T12626	T12625	arg1Of	sequence,gene
R9880	T12626	T12627	arg2Of	sequence,used
R9881	T12627	T12628	arg1Of	used,in
R9882	T12629	T12630	arg1Of	the,targeting
R9883	T12630	T12628	arg2Of	targeting,in
R9884	T12630	T12629	arg1Of	targeting,the
R9885	T12631	T12630	arg2Of	construct,targeting
R9886	T12631	T12632	arg1Of	construct,(
R9887	T12634	T12632	arg2Of	1A,(
R9888	T12634	T12633	arg1Of	1A,Fig.
R9889	T12635	T12632	arg3Of	),(
R9890	T12637	T12636	arg2Of	amplified,were
R9891	T12639	T12637	arg1Of	PCR,amplified
R9892	T12639	T12638	arg2Of	PCR,by
R9893	T12639	T12640	arg1Of	PCR,from
R9894	T12643	T12640	arg2Of	DNA,from
R9895	T12643	T12641	arg1Of	DNA,A20.2J
R9896	T12643	T12642	arg1Of	DNA,genomic
R9897	T12646	T12644	arg1Of	primers,The
R9898	T12646	T12645	arg1Of	primers,oligonucleotide
R9899	T12646	T12647	arg2Of	primers,used
R9900	T12646	T12649	arg1Of	primers,generate
R9901	T12646	T12657	arg1Of	primers,were
R9902	T12646	T12658	arg1Of	primers,5′-ttttctagagcggtggcttaagtgaccc-3′
R9903	T12646	T12660	arg1Of	primers,5′-tattctagatgcagcatctgagaaagcaagc-3′
R9904	T12649	T12647	arg3Of	generate,used
R9905	T12649	T12648	arg1Of	generate,to
R9906	T12652	T12649	arg2Of	flank,generate
R9907	T12652	T12650	arg1Of	flank,the
R9908	T12652	T12651	arg1Of	flank,5′
R9909	T12652	T12653	arg1Of	flank,(
R9910	T12655	T12653	arg2Of	bp,(
R9911	T12655	T12654	arg1Of	bp,1224
R9912	T12656	T12653	arg3Of	),(
R9913	T12658	T12659	arg1Of	5′-ttttctagagcggtggcttaagtgaccc-3′,and
R9914	T12659	T12657	arg2Of	and,were
R9915	T12660	T12659	arg2Of	5′-tattctagatgcagcatctgagaaagcaagc-3′,and
R9916	T12663	T12661	arg1Of	flank,The
R9917	T12663	T12662	arg1Of	flank,3′
R9918	T12663	T12664	arg1Of	flank,(
R9919	T12666	T12664	arg2Of	bp,(
R9920	T12666	T12665	arg1Of	bp,6032
R9921	T12667	T12664	arg3Of	),(
R9922	T12668	T12663	arg1Of	primers,flank
R9923	T12668	T12669	arg1Of	primers,were
R9924	T12668	T12670	arg1Of	primers,5′-aaaaccggtgtatgcttctttacgggagaaaaatattag-3′
R9925	T12668	T12672	arg1Of	primers,5′-tataccggtatgaagccaaaggaacactgagag-3′
R9926	T12670	T12671	arg1Of	5′-aaaaccggtgtatgcttctttacgggagaaaaatattag-3′,and
R9927	T12671	T12669	arg2Of	and,were
R9928	T12672	T12671	arg2Of	5′-tataccggtatgaagccaaaggaacactgagag-3′,and
R9929	T12675	T12673	arg1Of	sites,Restriction
R9930	T12675	T12674	arg1Of	sites,endonuclease
R9931	T12675	T12676	arg1Of	sites,in
R9932	T12675	T12680	arg1Of	sites,allowed
R9933	T12679	T12676	arg2Of	primers,in
R9934	T12679	T12677	arg1Of	primers,the
R9935	T12679	T12678	arg1Of	primers,oligonucleotide
R9936	T12681	T12680	arg2Of	insertion,allowed
R9937	T12681	T12682	arg1Of	insertion,of
R9938	T12685	T12682	arg2Of	products,of
R9939	T12685	T12683	arg1Of	products,the
R9940	T12685	T12684	arg1Of	products,PCR
R9941	T12685	T12686	arg1Of	products,into
R9942	T12689	T12686	arg2Of	vector,into
R9943	T12689	T12687	arg1Of	vector,the
R9944	T12689	T12688	arg1Of	vector,targeting
R9945	T12691	T12690	arg1Of	cells,A20.2J
R9946	T12691	T12692	arg1Of	cells,were
R9947	T12691	T12693	arg2Of	cells,transfected
R9948	T12691	T12706	arg2Of	cells,subcloned
R9949	T12693	T12694	arg1Of	transfected,(
R9950	T12693	T12701	arg1Of	transfected,with
R9951	T12693	T12705	arg1Of	transfected,and
R9952	T12695	T12694	arg2Of	by,(
R9953	T12696	T12695	arg2Of	electroporation,by
R9954	T12696	T12697	arg1Of	electroporation,[
R9955	T12698	T12697	arg2Of	7,[
R9956	T12699	T12697	arg3Of	],[
R9957	T12700	T12694	arg3Of	),(
R9958	T12702	T12703	arg1Of	the,targeting
R9959	T12703	T12701	arg2Of	targeting,with
R9960	T12703	T12702	arg1Of	targeting,the
R9961	T12704	T12703	arg2Of	construct,targeting
R9962	T12705	T12692	arg2Of	and,were
R9963	T12706	T12705	arg2Of	subcloned,and
R9964	T12706	T12707	arg1Of	subcloned,in
R9965	T12708	T12707	arg2Of	medium,in
R9966	T12708	T12709	arg1Of	medium,containing
R9967	T12713	T12709	arg2Of	sulfate,containing
R9968	T12713	T12710	arg1Of	sulfate,600
R9969	T12713	T12711	arg1Of	sulfate,µg/ml
R9970	T12713	T12712	arg1Of	sulfate,G418
R9971	T12715	T12714	arg1Of	recombination,Homologous
R9972	T12715	T12716	arg1Of	recombination,in
R9973	T12715	T12719	arg1Of	recombination,was
R9974	T12715	T12720	arg2Of	recombination,detected
R9975	T12718	T12716	arg2Of	clones,in
R9976	T12718	T12717	arg1Of	clones,G418-resistant
R9977	T12720	T12719	arg2Of	detected,was
R9978	T12720	T12726	arg1Of	detected,","
R9979	T12720	T12727	arg1Of	detected,as
R9980	T12722	T12720	arg1Of	PCR,detected
R9981	T12722	T12721	arg2Of	PCR,by
R9982	T12722	T12723	arg1Of	PCR,of
R9983	T12725	T12723	arg2Of	DNA,of
R9984	T12725	T12724	arg1Of	DNA,genomic
R9985	T12728	T12727	arg2Of	described,as
R9986	T12728	T12729	arg1Of	described,[
R9987	T12730	T12729	arg2Of	7,[
R9988	T12731	T12729	arg3Of	],[
R9989	T12733	T12732	arg1Of	primers,Oligonucleotide
R9990	T12733	T12734	arg2Of	primers,used
R9991	T12733	T12737	arg1Of	primers,were
R9992	T12733	T12738	arg1Of	primers,5′-cttcctgatctcagctttaccgtcac-3′
R9993	T12733	T12753	arg1Of	primers,5′-caatccatcttgttcagccat-3′
R9994	T12734	T12735	arg1Of	used,for
R9995	T12736	T12735	arg2Of	screening,for
R9996	T12738	T12739	arg1Of	5′-cttcctgatctcagctttaccgtcac-3′,(
R9997	T12738	T12752	arg1Of	5′-cttcctgatctcagctttaccgtcac-3′,and
R9998	T12740	T12741	arg1Of	homologous,to
R9999	T12740	T12744	arg1Of	homologous,;
R10000	T12743	T12741	arg2Of	sequence,to
R10001	T12743	T12742	arg1Of	sequence,genomic
R10002	T12745	T12744	arg2Of	approximate,;
R10003	T12746	T12739	arg2Of	position,(
R10004	T12746	T12740	arg1Of	position,homologous
R10005	T12746	T12745	arg1Of	position,approximate
R10006	T12746	T12747	arg2Of	position,noted
R10007	T12747	T12748	arg1Of	noted,in
R10008	T12749	T12748	arg2Of	Fig.,in
R10009	T12749	T12750	arg1Of	Fig.,1
R10010	T12751	T12739	arg3Of	),(
R10011	T12752	T12737	arg2Of	and,were
R10012	T12753	T12752	arg2Of	5′-caatccatcttgttcagccat-3′,and
R10013	T12753	T12754	arg1Of	5′-caatccatcttgttcagccat-3′,(
R10014	T12757	T12754	arg2Of	sequence,(
R10015	T12757	T12755	arg1Of	sequence,homologous
R10016	T12757	T12756	arg1Of	sequence,to
R10017	T12757	T12758	arg1Of	sequence,in
R10018	T12759	T12758	arg2Of	NeoR,in
R10019	T12760	T12754	arg3Of	),(
R10020	T12761	T12762	arg2Of	Clones,in
R10021	T12761	T12763	arg1Of	Clones,which
R10022	T12761	T12771	arg1Of	Clones,were
R10023	T12761	T12773	arg2Of	Clones,transfected
R10024	T12761	T12784	arg1Of	Clones,remove
R10025	T12765	T12764	arg1Of	copy,one
R10026	T12765	T12766	arg1Of	copy,of
R10027	T12765	T12768	arg1Of	copy,had
R10028	T12765	T12769	arg1Of	copy,been
R10029	T12765	T12770	arg2Of	copy,disrupted
R10030	T12767	T12766	arg2Of	Rnf31,of
R10031	T12770	T12762	arg1Of	disrupted,in
R10032	T12770	T12768	arg2Of	disrupted,had
R10033	T12770	T12769	arg2Of	disrupted,been
R10034	T12773	T12771	arg2Of	transfected,were
R10035	T12773	T12772	arg1Of	transfected,transiently
R10036	T12773	T12774	arg1Of	transfected,with
R10037	T12773	T12780	arg1Of	transfected,","
R10038	T12773	T12781	arg1Of	transfected,in
R10039	T12777	T12774	arg2Of	plasmid,with
R10040	T12777	T12775	arg1Of	plasmid,an
R10041	T12777	T12776	arg1Of	plasmid,expression
R10042	T12777	T12778	arg1Of	plasmid,encoding
R10043	T12779	T12778	arg2Of	Cre,encoding
R10044	T12782	T12781	arg2Of	order,in
R10045	T12784	T12781	arg3Of	remove,in
R10046	T12784	T12783	arg1Of	remove,to
R10047	T12785	T12784	arg2Of	NeoR,remove
R10048	T12787	T12786	arg1Of	subclones,G418-sensitive
R10049	T12787	T12788	arg1Of	subclones,were
R10050	T12787	T12789	arg2Of	subclones,subjected
R10051	T12789	T12788	arg2Of	subjected,were
R10052	T12789	T12790	arg1Of	subjected,to
R10053	T12789	T12796	modOf	subjected,to
R10054	T12793	T12790	arg2Of	round,to
R10055	T12793	T12791	arg1Of	round,a
R10056	T12793	T12792	arg1Of	round,second
R10057	T12793	T12794	arg1Of	round,of
R10058	T12795	T12794	arg2Of	targeting,of
R10059	T12797	T12796	arg1Of	disrupt,to
R10060	T12800	T12797	arg2Of	copy,disrupt
R10061	T12800	T12798	arg1Of	copy,the
R10062	T12800	T12799	arg1Of	copy,remaining
R10063	T12800	T12801	arg1Of	copy,of
R10064	T12802	T12801	arg2Of	Rnf31,of
R10065	T12804	T12803	arg1Of	clones,G418-resistant
R10066	T12804	T12805	arg1Of	clones,were
R10067	T12804	T12806	arg2Of	clones,tested
R10068	T12806	T12805	arg2Of	tested,were
R10069	T12806	T12807	arg1Of	tested,for
R10070	T12809	T12807	arg2Of	recombination,for
R10071	T12809	T12808	arg1Of	recombination,homologous
R10072	T12811	T12812	arg1Of	PCR,and
R10073	T12813	T12812	arg2Of	HOIP,and
R10074	T12815	T12806	arg1Of	expression,tested
R10075	T12815	T12810	arg2Of	expression,by
R10076	T12815	T12811	arg1Of	expression,PCR
R10077	T12815	T12813	arg1Of	expression,HOIP
R10078	T12815	T12814	arg1Of	expression,protein
R10079	T12815	T12816	arg1Of	expression,by
R10080	T12818	T12816	arg2Of	blot,by
R10081	T12818	T12817	arg1Of	blot,Western
R10292	T13107	T13106	arg1Of	transduction,Retroviral
R10293	T13110	T13108	arg1Of	vector,A
R10294	T13110	T13109	arg1Of	vector,retroviral
R10295	T13110	T13111	arg1Of	vector,(
R10296	T13110	T13114	arg1Of	vector,was
R10297	T13110	T13115	arg2Of	vector,used
R10298	T13112	T13111	arg2Of	pMIP,(
R10299	T13113	T13111	arg3Of	),(
R10300	T13115	T13114	arg2Of	used,was
R10301	T13115	T13116	arg1Of	used,for
R10302	T13115	T13124	arg1Of	used,and
R10303	T13119	T13116	arg2Of	transduction,for
R10304	T13119	T13117	arg1Of	transduction,the
R10305	T13119	T13118	arg1Of	transduction,stable
R10306	T13119	T13120	arg1Of	transduction,of
R10307	T13119	T13122	arg1Of	transduction,with
R10308	T13121	T13120	arg2Of	cells,of
R10309	T13123	T13122	arg2Of	HOIP,with
R10310	T13128	T13125	arg1Of	pMIP,IKKγ
R10311	T13128	T13126	arg1Of	pMIP,cDNA
R10312	T13128	T13127	arg1Of	pMIP,constructs.
R10313	T13128	T13129	arg1Of	pMIP,was
R10314	T13128	T13130	arg2Of	pMIP,constructed
R10315	T13130	T13124	arg2Of	constructed,and
R10316	T13130	T13129	arg2Of	constructed,was
R10317	T13130	T13131	arg1Of	constructed,by
R10318	T13132	T13131	arg2Of	replacing,by
R10319	T13132	T13141	arg1Of	replacing,[
R10320	T13132	T13144	arg1Of	replacing,with
R10321	T13134	T13135	arg1Of	IRES,and
R10322	T13135	T13132	arg2Of	and,replacing
R10323	T13135	T13133	arg1Of	and,the
R10324	T13135	T13137	arg1Of	and,encoding
R10325	T13136	T13135	arg2Of	GFP,and
R10326	T13137	T13139	arg1Of	encoding,in
R10327	T13138	T13137	arg2Of	sequences,encoding
R10328	T13140	T13139	arg2Of	pMIG,in
R10329	T13142	T13141	arg2Of	31,[
R10330	T13143	T13141	arg3Of	],[
R10331	T13146	T13147	arg1Of	IRES,and
R10332	T13148	T13147	arg2Of	puromycin,and
R10333	T13150	T13144	arg2Of	gene,with
R10334	T13150	T13145	arg1Of	gene,the
R10335	T13150	T13146	arg1Of	gene,IRES
R10336	T13150	T13148	arg1Of	gene,puromycin
R10337	T13150	T13149	arg1Of	gene,resistance
R10338	T13150	T13151	arg1Of	gene,from
R10339	T13152	T13151	arg2Of	pIRESpuro2,from
R10340	T13152	T13153	arg1Of	pIRESpuro2,(
R10341	T13154	T13153	arg2Of	Clontech,(
R10342	T13155	T13153	arg3Of	),(
R10343	T13159	T13156	arg1Of	cDNA,FLAG-tagged
R10344	T13159	T13157	arg1Of	cDNA,mouse
R10345	T13159	T13158	arg1Of	cDNA,HOIP
R10346	T13159	T13160	arg1Of	cDNA,was
R10347	T13159	T13161	arg2Of	cDNA,inserted
R10348	T13161	T13160	arg2Of	inserted,was
R10349	T13161	T13162	arg1Of	inserted,into
R10350	T13166	T13162	arg2Of	site,into
R10351	T13166	T13163	arg1Of	site,the
R10352	T13166	T13164	arg1Of	site,multiple
R10353	T13166	T13165	arg1Of	site,cloning
R10354	T13166	T13167	arg1Of	site,of
R10355	T13168	T13167	arg2Of	pMIP,of
R10356	T13171	T13169	arg1Of	construct,A
R10357	T13171	T13170	arg1Of	construct,pMIP
R10358	T13171	T13172	arg1Of	construct,encoding
R10359	T13171	T13186	arg1Of	construct,was
R10360	T13171	T13188	arg2Of	construct,prepared
R10361	T13172	T13175	arg1Of	encoding,with
R10362	T13174	T13172	arg2Of	IKKγ,encoding
R10363	T13174	T13173	arg1Of	IKKγ,mouse
R10364	T13181	T13177	arg1Of	peptide,amino
R10365	T13181	T13178	arg1Of	peptide,terminal
R10366	T13181	T13179	arg1Of	peptide,birch
R10367	T13181	T13180	arg1Of	peptide,profilin
R10368	T13181	T13182	arg1Of	peptide,(
R10369	T13183	T13182	arg2Of	BP,(
R10370	T13184	T13182	arg3Of	),(
R10371	T13185	T13175	arg2Of	-tag,with
R10372	T13185	T13176	arg1Of	-tag,an
R10373	T13185	T13181	arg1Of	-tag,peptide
R10374	T13188	T13186	arg2Of	prepared,was
R10375	T13188	T13187	arg1Of	prepared,also
R10376	T13190	T13189	arg1Of	particles,Retroviral
R10377	T13190	T13191	arg1Of	particles,were
R10378	T13190	T13192	arg2Of	particles,generated
R10379	T13192	T13191	arg2Of	generated,were
R10380	T13192	T13193	arg1Of	generated,by
R10381	T13195	T13193	arg2Of	transfection,by
R10382	T13195	T13194	arg1Of	transfection,transient
R10383	T13195	T13196	arg1Of	transfection,of
R10384	T13195	T13199	arg1Of	transfection,with
R10385	T13198	T13196	arg2Of	cells,of
R10386	T13198	T13197	arg1Of	cells,293T
R10387	T13200	T13201	arg1Of	pMIP,","
R10388	T13201	T13204	arg1Of	",",or
R10389	T13202	T13201	arg2Of	pMIP-HOIP,","
R10390	T13204	T13203	arg1Of	or,","
R10391	T13204	T13206	arg1Of	or,and
R10392	T13205	T13204	arg2Of	pMIP-IKKγ,or
R10393	T13206	T13199	arg2Of	and,with
R10394	T13210	T13206	arg2Of	pCL-Eco,and
R10395	T13210	T13207	arg1Of	pCL-Eco,the
R10396	T13210	T13208	arg1Of	pCL-Eco,packaging
R10397	T13210	T13209	arg1Of	pCL-Eco,vector
R10398	T13210	T13211	arg1Of	pCL-Eco,[
R10399	T13212	T13211	arg2Of	32,[
R10400	T13213	T13211	arg3Of	],[
R10401	T13215	T13214	arg1Of	days,Two
R10402	T13216	T13215	arg1Of	after,days
R10403	T13217	T13216	arg2Of	transfection,after
R10404	T13220	T13219	arg1Of	supernatants,culture
R10405	T13220	T13221	arg1Of	supernatants,were
R10406	T13220	T13222	arg2Of	supernatants,harvested
R10407	T13220	T13224	arg2Of	supernatants,filtered
R10408	T13222	T13223	arg1Of	harvested,and
R10409	T13223	T13216	arg1Of	and,after
R10410	T13223	T13218	arg1Of	and,","
R10411	T13223	T13221	arg2Of	and,were
R10412	T13224	T13223	arg2Of	filtered,and
R10413	T13225	T13226	arg1Of	A20.2J,or
R10414	T13227	T13226	arg2Of	HOIP-deficient,or
R10415	T13228	T13225	arg1Of	cells,A20.2J
R10416	T13228	T13227	arg1Of	cells,HOIP-deficient
R10417	T13228	T13229	arg1Of	cells,were
R10418	T13228	T13230	arg2Of	cells,added
R10419	T13230	T13229	arg2Of	added,were
R10420	T13230	T13231	arg1Of	added,to
R10421	T13230	T13238	arg1Of	added,with
R10422	T13233	T13231	arg2Of	plates,to
R10423	T13233	T13232	arg1Of	plates,24-well
R10424	T13233	T13234	arg1Of	plates,(
R10425	T13236	T13234	arg2Of	cells/well,(
R10426	T13236	T13235	arg1Of	cells/well,2.5×105
R10427	T13237	T13234	arg3Of	),(
R10428	T13242	T13239	arg1Of	supernatant,1
R10429	T13242	T13240	arg1Of	supernatant,ml
R10430	T13242	T13241	arg1Of	supernatant,virus-containing
R10431	T13242	T13243	arg1Of	supernatant,and
R10432	T13243	T13238	arg2Of	and,with
R10433	T13246	T13243	arg2Of	polybrene,and
R10434	T13246	T13244	arg1Of	polybrene,8
R10435	T13246	T13245	arg1Of	polybrene,µg/ml
R10436	T13247	T13248	arg1Of	Plates,were
R10437	T13247	T13249	arg2Of	Plates,centrifuged
R10438	T13249	T13248	arg2Of	centrifuged,were
R10439	T13249	T13250	arg1Of	centrifuged,at
R10440	T13249	T13254	arg1Of	centrifuged,for
R10441	T13253	T13250	arg2Of	g,at
R10442	T13253	T13251	arg1Of	g,500
R10443	T13253	T13252	arg1Of	g,x
R10444	T13256	T13254	arg2Of	hrs,for
R10445	T13256	T13255	arg1Of	hrs,2
R10446	T13256	T13257	arg1Of	hrs,at
R10447	T13259	T13257	arg2Of	temperature,at
R10448	T13259	T13258	arg1Of	temperature,room
R10449	T13260	T13261	arg1Of	Cells,were
R10450	T13260	T13262	arg2Of	Cells,cultured
R10451	T13262	T13261	arg2Of	cultured,were
R10452	T13262	T13263	arg1Of	cultured,in
R10453	T13262	T13268	arg1Of	cultured,for
R10454	T13267	T13263	arg2Of	medium,in
R10455	T13267	T13264	arg1Of	medium,B
R10456	T13267	T13265	arg1Of	medium,cell
R10457	T13267	T13266	arg1Of	medium,culture
R10458	T13270	T13268	arg2Of	hrs,for
R10459	T13270	T13269	arg1Of	hrs,48
R10460	T13270	T13271	arg1Of	hrs,","
R10461	T13270	T13272	arg2Of	hrs,after
R10462	T13270	T13273	arg1Of	hrs,which
R10463	T13276	T13274	arg1Of	cells,the
R10464	T13276	T13275	arg2Of	cells,transduced
R10465	T13276	T13277	arg1Of	cells,were
R10466	T13276	T13278	arg2Of	cells,selected
R10467	T13278	T13272	arg1Of	selected,after
R10468	T13278	T13277	arg2Of	selected,were
R10469	T13278	T13279	arg1Of	selected,in
R10470	T13282	T13279	arg2Of	puromycin,in
R10471	T13282	T13280	arg1Of	puromycin,3
R10472	T13282	T13281	arg1Of	puromycin,µg/ml
R10560	T13428	T13427	arg1Of	upregulation,CD80
R10561	T13428	T13429	arg1Of	upregulation,and
R10562	T13431	T13429	arg2Of	cytometry,and
R10563	T13431	T13430	arg1Of	cytometry,flow
R10564	T13432	T13433	arg1Of	Cells,(
R10565	T13432	T13439	arg1Of	Cells,were
R10566	T13432	T13440	arg2Of	Cells,cultured
R10567	T13434	T13433	arg2Of	5×104,(
R10568	T13434	T13435	arg1Of	5×104,in
R10569	T13437	T13435	arg2Of	ml,in
R10570	T13437	T13436	arg1Of	ml,2
R10571	T13438	T13433	arg3Of	),(
R10572	T13440	T13439	arg2Of	cultured,were
R10573	T13440	T13441	arg1Of	cultured,in
R10574	T13443	T13441	arg2Of	plates,in
R10575	T13443	T13442	arg1Of	plates,24-well
R10576	T13443	T13444	arg1Of	plates,for
R10577	T13446	T13444	arg2Of	days,for
R10578	T13446	T13445	arg1Of	days,3
R10579	T13446	T13447	arg1Of	days,with
R10580	T13450	T13448	arg1Of	anti-CD40,5
R10581	T13450	T13449	arg1Of	anti-CD40,µg/ml
R10582	T13450	T13451	arg1Of	anti-CD40,or
R10583	T13451	T13447	arg2Of	or,with
R10584	T13455	T13451	arg2Of	antibody,or
R10585	T13455	T13452	arg1Of	antibody,an
R10586	T13455	T13453	arg1Of	antibody,isotype
R10587	T13455	T13454	arg1Of	antibody,control
R10588	T13456	T13457	arg1Of	Cells,were
R10589	T13456	T13458	arg2Of	Cells,stained
R10590	T13458	T13457	arg2Of	stained,were
R10591	T13458	T13459	arg1Of	stained,for
R10592	T13461	T13460	arg1Of	cytometry,flow
R10593	T13461	T13462	arg1Of	cytometry,with
R10594	T13461	T13464	arg1Of	cytometry,or
R10595	T13463	T13462	arg2Of	FITC-anti-CD80,with
R10596	T13464	T13459	arg2Of	or,for
R10597	T13468	T13464	arg2Of	antibody,or
R10598	T13468	T13465	arg1Of	antibody,an
R10599	T13468	T13466	arg1Of	antibody,appropriate
R10600	T13468	T13467	arg1Of	antibody,control
R10601	T13468	T13470	arg2Of	antibody,described
R10602	T13470	T13469	arg1Of	described,as
R10603	T13470	T13471	arg1Of	described,[
R10604	T13472	T13471	arg2Of	28,[
R10605	T13473	T13471	arg3Of	],[
R10606	T13474	T13475	arg2Of	Data,acquired
R10607	T13474	T13485	arg1Of	Data,were
R10608	T13474	T13486	arg2Of	Data,analyzed
R10609	T13475	T13476	arg1Of	acquired,with
R10610	T13480	T13476	arg2Of	cytometer,with
R10611	T13480	T13477	arg1Of	cytometer,a
R10612	T13480	T13478	arg1Of	cytometer,FACScan
R10613	T13480	T13479	arg1Of	cytometer,flow
R10614	T13480	T13481	arg1Of	cytometer,(
R10615	T13483	T13481	arg2Of	Biosciences,(
R10616	T13483	T13482	arg1Of	Biosciences,BD
R10617	T13484	T13481	arg3Of	),(
R10618	T13486	T13485	arg2Of	analyzed,were
R10619	T13486	T13487	arg1Of	analyzed,with
R10620	T13488	T13487	arg2Of	WinMDI,with
R10621	T13488	T13489	arg1Of	WinMDI,2.8
R10622	T13488	T13490	arg1Of	WinMDI,(
R10623	T13493	T13490	arg2Of	Institute,(
R10624	T13493	T13491	arg1Of	Institute,Scripps
R10625	T13493	T13492	arg1Of	Institute,Research
R10626	T13493	T13494	arg1Of	Institute,","
R10627	T13496	T13494	arg2Of	Diego,","
R10628	T13496	T13495	arg1Of	Diego,San
R10629	T13496	T13497	arg1Of	Diego,","
R10630	T13498	T13497	arg2Of	CA,","
R10631	T13499	T13490	arg3Of	),(
R10768	T13686	T13684	arg1Of	assay,GLε
R10769	T13686	T13685	arg1Of	assay,transcript
R10770	T13689	T13687	arg1Of	activation,The
R10771	T13689	T13688	arg1Of	activation,CD40-simulated
R10772	T13689	T13690	arg1Of	activation,of
R10773	T13689	T13693	arg1Of	activation,was
R10774	T13689	T13694	arg2Of	activation,evaluated
R10775	T13692	T13690	arg2Of	transcription,of
R10776	T13692	T13691	arg1Of	transcription,GLε
R10777	T13694	T13693	arg2Of	evaluated,was
R10778	T13694	T13695	arg1Of	evaluated,as
R10779	T13697	T13696	arg1Of	reported,previously
R10780	T13699	T13695	arg2Of	33,as
R10781	T13699	T13697	arg1Of	33,reported
R10782	T13699	T13698	arg2Of	33,[
R10783	T13700	T13698	arg3Of	],[
R10784	T13704	T13703	arg1Of	cells,1×106
R10785	T13704	T13705	arg1Of	cells,were
R10786	T13704	T13706	arg2Of	cells,stimulated
R10787	T13706	T13701	arg1Of	stimulated,Briefly
R10788	T13706	T13702	arg1Of	stimulated,","
R10789	T13706	T13705	arg2Of	stimulated,were
R10790	T13706	T13707	arg1Of	stimulated,overnight
R10791	T13706	T13708	arg1Of	stimulated,with
R10792	T13706	T13720	arg1Of	stimulated,","
R10793	T13706	T13721	arg1Of	stimulated,with
R10794	T13706	T13723	arg1Of	stimulated,without
R10795	T13710	T13709	arg1Of	antibody,anti-CD40
R10796	T13710	T13711	arg1Of	antibody,(
R10797	T13710	T13715	arg1Of	antibody,or
R10798	T13713	T13711	arg2Of	µg/ml,(
R10799	T13713	T13712	arg1Of	µg/ml,10
R10800	T13714	T13711	arg3Of	),(
R10801	T13715	T13708	arg2Of	or,with
R10802	T13719	T13715	arg2Of	antibody,or
R10803	T13719	T13716	arg1Of	antibody,an
R10804	T13719	T13717	arg1Of	antibody,isotype
R10805	T13719	T13718	arg1Of	antibody,control
R10806	T13721	T13722	arg1Of	with,or
R10807	T13723	T13722	arg2Of	without,or
R10808	T13727	T13721	arg2Of	IL-4,with
R10809	T13727	T13723	arg2Of	IL-4,without
R10810	T13727	T13724	arg1Of	IL-4,500
R10811	T13727	T13725	arg1Of	IL-4,U/ml
R10812	T13727	T13726	arg1Of	IL-4,mouse
R10813	T13727	T13728	arg1Of	IL-4,(
R10814	T13730	T13728	arg2Of	Biosciences,(
R10815	T13730	T13729	arg1Of	Biosciences,BD
R10816	T13731	T13728	arg3Of	),(
R10817	T13732	T13733	arg1Of	RNA,was
R10818	T13732	T13734	arg2Of	RNA,isolated
R10819	T13734	T13733	arg2Of	isolated,was
R10820	T13735	T13734	arg3Of	using,isolated
R10821	T13736	T13737	arg1Of	Trizol,(
R10822	T13736	T13741	arg1Of	Trizol,and
R10823	T13738	T13737	arg2Of	Invitrogen,(
R10824	T13739	T13737	arg3Of	),(
R10825	T13741	T13735	arg2Of	and,using
R10826	T13741	T13740	arg1Of	and,","
R10827	T13742	T13741	arg2Of	reverse-transcribed,and
R10828	T13742	T13743	arg1Of	reverse-transcribed,(
R10829	T13746	T13743	arg2Of	kit,(
R10830	T13746	T13744	arg1Of	kit,Superscript
R10831	T13746	T13745	arg1Of	kit,III
R10832	T13746	T13747	arg1Of	kit,","
R10833	T13748	T13747	arg2Of	Invitrogen,","
R10834	T13749	T13743	arg3Of	),(
R10835	T13751	T13750	arg1Of	PCR,Quantitative
R10836	T13751	T13752	arg1Of	PCR,for
R10837	T13751	T13756	arg1Of	PCR,was
R10838	T13751	T13757	arg2Of	PCR,performed
R10839	T13753	T13754	arg1Of	GLε,and
R10840	T13754	T13752	arg2Of	and,for
R10841	T13755	T13754	arg2Of	Hprt1,and
R10842	T13757	T13756	arg2Of	performed,was
R10843	T13758	T13757	arg3Of	using,performed
R10844	T13762	T13759	arg1Of	mix,SYBR
R10845	T13762	T13760	arg1Of	mix,GREEN
R10846	T13762	T13761	arg1Of	mix,master
R10847	T13762	T13763	arg1Of	mix,(
R10848	T13762	T13768	arg1Of	mix,and
R10849	T13765	T13763	arg2Of	Biosystems,(
R10850	T13765	T13764	arg1Of	Biosystems,Applied
R10851	T13766	T13763	arg3Of	),(
R10852	T13768	T13758	arg2Of	and,using
R10853	T13768	T13767	arg1Of	and,","
R10854	T13774	T13770	arg1Of	Real-Time,Applied
R10855	T13774	T13771	arg1Of	Real-Time,Biosystems
R10856	T13774	T13772	arg1Of	Real-Time,7900HT
R10857	T13774	T13773	arg1Of	Real-Time,Fast
R10858	T13776	T13768	arg2Of	instrument,and
R10859	T13776	T13769	arg1Of	instrument,an
R10860	T13776	T13774	arg1Of	instrument,Real-Time
R10861	T13776	T13775	arg1Of	instrument,PCR
R10862	T13777	T13778	arg1Of	Expression,of
R10863	T13777	T13780	arg1Of	Expression,in
R10864	T13777	T13783	arg1Of	Expression,was
R10865	T13777	T13784	arg2Of	Expression,normalized
R10866	T13779	T13778	arg2Of	GLε,of
R10867	T13782	T13780	arg2Of	sample,in
R10868	T13782	T13781	arg1Of	sample,each
R10869	T13784	T13783	arg2Of	normalized,was
R10870	T13784	T13785	arg1Of	normalized,to
R10871	T13787	T13785	arg2Of	expression,to
R10872	T13787	T13786	arg1Of	expression,the
R10873	T13787	T13788	arg1Of	expression,of
R10874	T13789	T13788	arg2Of	Hprt1,of
R11080	T14056	T14057	arg1Of	NF-κB,and
R11081	T14058	T14057	arg2Of	JNK,and
R11082	T14060	T14056	arg1Of	assays,NF-κB
R11083	T14060	T14058	arg1Of	assays,JNK
R11084	T14060	T14059	arg1Of	assays,activation
R11085	T14062	T14061	arg2Of	activation,For
R11086	T14062	T14063	arg1Of	activation,of
R11087	T14065	T14063	arg2Of	signaling,of
R11088	T14065	T14064	arg1Of	signaling,CD40
R11089	T14070	T14067	arg1Of	cells,5×104
R11090	T14070	T14068	arg1Of	cells,HI5
R11091	T14070	T14069	arg1Of	cells,insect
R11092	T14070	T14071	arg1Of	cells,or
R11093	T14071	T14075	arg1Of	or,expressing
R11094	T14071	T14082	arg1Of	or,were
R11095	T14071	T14083	arg2Of	or,added
R11096	T14074	T14071	arg2Of	cells,or
R11097	T14074	T14072	arg1Of	cells,HI5
R11098	T14074	T14073	arg1Of	cells,insect
R11099	T14077	T14075	arg2Of	CD154,expressing
R11100	T14077	T14076	arg1Of	CD154,mouse
R11101	T14077	T14078	arg1Of	CD154,(
R11102	T14080	T14078	arg2Of	ligand,(
R11103	T14080	T14079	arg1Of	ligand,CD40
R11104	T14081	T14078	arg3Of	),(
R11105	T14083	T14061	arg1Of	added,For
R11106	T14083	T14066	arg1Of	added,","
R11107	T14083	T14082	arg2Of	added,were
R11108	T14083	T14084	arg1Of	added,to
R11109	T14087	T14084	arg2Of	cells,to
R11110	T14087	T14085	arg1Of	cells,1×106
R11111	T14087	T14086	arg1Of	cells,B
R11112	T14088	T14089	arg1Of	Cells,were
R11113	T14088	T14090	arg2Of	Cells,centrifuged
R11114	T14090	T14089	arg2Of	centrifuged,were
R11115	T14090	T14091	arg1Of	centrifuged,for
R11116	T14090	T14094	arg1Of	centrifuged,at
R11117	T14090	T14099	modOf	centrifuged,to
R11118	T14093	T14091	arg2Of	minute,for
R11119	T14093	T14092	arg1Of	minute,1
R11120	T14097	T14094	arg2Of	g,at
R11121	T14097	T14095	arg1Of	g,400
R11122	T14097	T14096	arg1Of	g,x
R11123	T14097	T14098	arg1Of	g,(
R11124	T14100	T14099	arg1Of	promote,to
R11125	T14101	T14100	arg2Of	contact,promote
R11126	T14101	T14102	arg1Of	contact,between
R11127	T14104	T14103	arg1Of	cells,ligand
R11128	T14104	T14105	arg1Of	cells,and
R11129	T14105	T14102	arg2Of	and,between
R11130	T14105	T14108	arg1Of	and,)
R11131	T14105	T14110	arg2Of	and,incubated
R11132	T14107	T14105	arg2Of	cells,and
R11133	T14107	T14106	arg1Of	cells,B
R11134	T14110	T14109	arg1Of	incubated,then
R11135	T14110	T14111	arg1Of	incubated,at
R11136	T14112	T14111	arg2Of	37°C,at
R11137	T14112	T14113	arg1Of	37°C,for
R11138	T14114	T14113	arg2Of	times,for
R11139	T14114	T14115	arg2Of	times,indicated
R11140	T14115	T14116	arg1Of	indicated,in
R11141	T14117	T14116	arg2Of	Fig.,in
R11142	T14117	T14118	arg1Of	Fig.,4
R11143	T14120	T14119	arg2Of	stimulation,Following
R11144	T14122	T14123	arg1Of	cells,were
R11145	T14122	T14124	arg2Of	cells,chilled
R11146	T14124	T14119	arg1Of	chilled,Following
R11147	T14124	T14121	arg1Of	chilled,","
R11148	T14124	T14123	arg2Of	chilled,were
R11149	T14124	T14125	arg1Of	chilled,on
R11150	T14124	T14127	arg1Of	chilled,for
R11151	T14126	T14125	arg2Of	ice,on
R11152	T14129	T14127	arg2Of	minutes,for
R11153	T14129	T14128	arg1Of	minutes,2
R11154	T14131	T14130	arg1Of	pellets,Cell
R11155	T14131	T14132	arg1Of	pellets,were
R11156	T14131	T14133	arg2Of	pellets,dissolved
R11157	T14131	T14140	arg2Of	pellets,sonicated
R11158	T14131	T14143	arg2Of	pellets,heated
R11159	T14133	T14134	arg1Of	dissolved,in
R11160	T14133	T14139	arg1Of	dissolved,","
R11161	T14136	T14134	arg2Of	SDS-PAGE,in
R11162	T14136	T14135	arg1Of	SDS-PAGE,2X
R11163	T14136	T14137	arg1Of	SDS-PAGE,loading
R11164	T14138	T14137	arg2Of	buffer,loading
R11165	T14139	T14142	arg1Of	",",and
R11166	T14140	T14139	arg2Of	sonicated,","
R11167	T14142	T14132	arg2Of	and,were
R11168	T14142	T14141	arg1Of	and,","
R11169	T14143	T14142	arg2Of	heated,and
R11170	T14143	T14144	arg1Of	heated,for
R11171	T14146	T14144	arg2Of	minutes,for
R11172	T14146	T14145	arg1Of	minutes,5
R11173	T14146	T14147	arg1Of	minutes,at
R11174	T14149	T14148	arg1Of	Total,95°C.
R11175	T14149	T14159	modOf	Total,were
R11176	T14151	T14150	arg1Of	lysates,cell
R11177	T14151	T14152	arg1Of	lysates,(
R11178	T14151	T14159	arg1Of	lysates,were
R11179	T14151	T14160	arg2Of	lysates,fractionated
R11180	T14151	T14164	arg2Of	lysates,blotted
R11181	T14155	T14152	arg2Of	equivalents,(
R11182	T14155	T14153	arg1Of	equivalents,1×105
R11183	T14155	T14154	arg1Of	equivalents,cell
R11184	T14155	T14156	arg1Of	equivalents,per
R11185	T14157	T14156	arg2Of	lane,per
R11186	T14158	T14152	arg3Of	),(
R11187	T14160	T14163	arg1Of	fractionated,and
R11188	T14162	T14160	arg1Of	SDS-PAGE,fractionated
R11189	T14162	T14161	arg2Of	SDS-PAGE,by
R11190	T14163	T14159	arg2Of	and,were
R11191	T14164	T14163	arg2Of	blotted,and
R11192	T14164	T14165	arg1Of	blotted,to
R11193	T14167	T14165	arg2Of	fluoride,to
R11194	T14167	T14166	arg1Of	fluoride,polyvinylidine
R11195	T14167	T14168	arg1Of	fluoride,(
R11196	T14169	T14168	arg2Of	PVDF,(
R11197	T14170	T14168	arg3Of	),(
R11198	T14171	T14147	arg2Of	membranes,at
R11199	T14171	T14149	arg1Of	membranes,Total
R11200	T14172	T14173	arg1Of	Membranes,were
R11201	T14172	T14174	arg2Of	Membranes,probed
R11202	T14174	T14173	arg2Of	probed,were
R11203	T14174	T14175	arg1Of	probed,with
R11204	T14174	T14177	arg1Of	probed,as
R11205	T14176	T14175	arg2Of	antibodies,with
R11206	T14178	T14177	arg2Of	indicated,as
R11207	T14178	T14179	arg1Of	indicated,in
R11208	T14180	T14179	arg2Of	Fig.,in
R11209	T14180	T14181	arg1Of	Fig.,4
R11210	T14183	T14182	arg1Of	detection,Chemiluminescent
R11211	T14183	T14184	arg1Of	detection,(
R11212	T14183	T14188	arg1Of	detection,was
R11213	T14183	T14189	arg2Of	detection,used
R11214	T14186	T14184	arg2Of	Biotechnology,(
R11215	T14186	T14185	arg1Of	Biotechnology,Pierce
R11216	T14187	T14184	arg3Of	),(
R11217	T14189	T14188	arg2Of	used,was
R11218	T14189	T14190	arg1Of	used,for
R11219	T14192	T14190	arg2Of	visualization,for
R11220	T14192	T14191	arg1Of	visualization,the
R11221	T14192	T14193	arg1Of	visualization,of
R11222	T14192	T14195	arg1Of	visualization,on
R11223	T14194	T14193	arg2Of	bands,of
R11224	T14197	T14195	arg2Of	blots,on
R11225	T14197	T14196	arg1Of	blots,Western
R11226	T14198	T14199	arg1Of	Images,of
R11227	T14198	T14201	arg1Of	Images,were
R11228	T14198	T14202	arg2Of	Images,recorded
R11229	T14200	T14199	arg2Of	blots,of
R11230	T14202	T14201	arg2Of	recorded,were
R11231	T14202	T14203	arg1Of	recorded,with
R11232	T14202	T14216	arg1Of	recorded,on
R11233	T14203	T14215	arg1Of	with,and
R11234	T14207	T14203	arg2Of	system,with
R11235	T14207	T14204	arg1Of	system,a
R11236	T14207	T14205	arg1Of	system,low-light
R11237	T14207	T14206	arg1Of	system,imaging
R11238	T14207	T14208	arg1Of	system,(
R11239	T14209	T14208	arg2Of	LAS4000,(
R11240	T14209	T14210	arg1Of	LAS4000,","
R11241	T14213	T14210	arg2Of	Systems,","
R11242	T14213	T14211	arg1Of	Systems,Fuji
R11243	T14213	T14212	arg1Of	Systems,Medical
R11244	T14214	T14208	arg3Of	),(
R11245	T14216	T14215	arg2Of	on,and
R11246	T14218	T14216	arg2Of	film,on
R11247	T14218	T14217	arg1Of	film,X-ray
R11475	T14503	T14504	arg1Of	Immunoprecipitation,of
R11476	T14503	T14506	arg1Of	Immunoprecipitation,was
R11477	T14503	T14507	arg2Of	Immunoprecipitation,performed
R11478	T14505	T14504	arg2Of	CD40,of
R11479	T14507	T14506	arg2Of	performed,was
R11480	T14511	T14507	arg1Of	capture,performed
R11481	T14511	T14508	arg2Of	capture,by
R11482	T14511	T14509	arg2Of	capture,activated
R11483	T14511	T14510	arg1Of	capture,receptor
R11484	T14511	T14512	arg1Of	capture,","
R11485	T14511	T14513	arg1Of	capture,as
R11486	T14515	T14513	arg2Of	described,as
R11487	T14515	T14514	arg1Of	described,previously
R11488	T14515	T14516	arg1Of	described,[
R11489	T14517	T14516	arg2Of	6,[
R11490	T14518	T14516	arg3Of	],[
R11491	T14519	T14520	arg1Of	Briefly,","
R11492	T14519	T14528	arg1Of	Briefly,were
R11493	T14519	T14529	arg2Of	Briefly,incubated
R11494	T14522	T14520	arg2Of	cells,","
R11495	T14522	T14521	arg1Of	cells,3×107
R11496	T14522	T14523	arg1Of	cells,(
R11497	T14524	T14525	arg1Of	A20.2J,and
R11498	T14525	T14523	arg2Of	and,(
R11499	T14526	T14525	arg2Of	derivatives,and
R11500	T14527	T14523	arg3Of	),(
R11501	T14529	T14528	arg2Of	incubated,were
R11502	T14529	T14530	arg1Of	incubated,for
R11503	T14532	T14531	arg1Of	minutes,60
R11504	T14532	T14533	arg1Of	minutes,at
R11505	T14532	T14536	arg1Of	minutes,with
R11506	T14532	T14564	arg1Of	minutes,or
R11507	T14535	T14533	arg2Of	temperature,at
R11508	T14535	T14534	arg1Of	temperature,room
R11509	T14542	T14536	arg2Of	beads,with
R11510	T14542	T14537	arg1Of	beads,10
R11511	T14542	T14538	arg1Of	beads,µl
R11512	T14542	T14539	arg1Of	beads,magnetic
R11513	T14542	T14540	arg1Of	beads,protein
R11514	T14542	T14541	arg1Of	beads,G
R11515	T14542	T14543	arg1Of	beads,(
R11516	T14542	T14546	arg1Of	beads,pre-coated
R11517	T14544	T14543	arg2Of	Dynal,(
R11518	T14545	T14543	arg3Of	),(
R11519	T14546	T14547	arg1Of	pre-coated,with
R11520	T14552	T14548	arg1Of	IgG,10
R11521	T14552	T14549	arg1Of	IgG,µg
R11522	T14552	T14550	arg1Of	IgG,goat
R11523	T14552	T14551	arg1Of	IgG,anti-rat
R11524	T14552	T14553	arg1Of	IgG,(
R11525	T14552	T14557	arg1Of	IgG,and
R11526	T14554	T14553	arg2Of	Jackson,(
R11527	T14555	T14553	arg3Of	),(
R11528	T14557	T14547	arg2Of	and,with
R11529	T14557	T14556	arg1Of	and,","
R11530	T14560	T14557	arg2Of	anti-CD40,and
R11531	T14560	T14558	arg1Of	anti-CD40,10
R11532	T14560	T14559	arg1Of	anti-CD40,µg
R11533	T14560	T14561	arg1Of	anti-CD40,(
R11534	T14562	T14561	arg2Of	1C10,(
R11535	T14563	T14561	arg3Of	),(
R11536	T14564	T14530	arg2Of	or,for
R11537	T14568	T14564	arg2Of	antibody,or
R11538	T14568	T14565	arg1Of	antibody,an
R11539	T14568	T14566	arg1Of	antibody,isotype
R11540	T14568	T14567	arg1Of	antibody,control
R11541	T14568	T14569	arg1Of	antibody,(
R11542	T14570	T14569	arg2Of	mAb72,(
R11543	T14571	T14569	arg3Of	),(
R11544	T14572	T14573	arg1Of	Cells/beads,were
R11545	T14572	T14575	arg2Of	Cells/beads,pelleted
R11546	T14572	T14579	arg2Of	Cells/beads,lysed
R11547	T14575	T14578	arg1Of	pelleted,and
R11548	T14577	T14575	arg1Of	centrifugation,pelleted
R11549	T14577	T14576	arg2Of	centrifugation,by
R11550	T14578	T14573	arg2Of	and,were
R11551	T14578	T14574	arg1Of	and,then
R11552	T14579	T14578	arg2Of	lysed,and
R11553	T14579	T14580	arg1Of	lysed,in
R11554	T14581	T14580	arg2Of	buffer,in
R11555	T14581	T14582	arg1Of	buffer,containing
R11556	T14583	T14584	arg1Of	1,%
R11557	T14586	T14582	arg2Of	X100,containing
R11558	T14586	T14583	arg1Of	X100,1
R11559	T14586	T14585	arg1Of	X100,Triton
R11560	T14587	T14588	arg1Of	Beads,were
R11561	T14587	T14589	arg2Of	Beads,washed
R11562	T14589	T14588	arg2Of	washed,were
R11563	T14589	T14590	arg1Of	washed,with
R11564	T14589	T14593	modOf	washed,to
R11565	T14592	T14590	arg2Of	buffer,with
R11566	T14592	T14591	arg1Of	buffer,lysis
R11567	T14594	T14593	arg1Of	remove,to
R11568	T14596	T14594	arg2Of	material,remove
R11569	T14596	T14595	arg1Of	material,unbound
R11570	T14599	T14597	arg2Of	experiments,In
R11571	T14599	T14598	arg1Of	experiments,some
R11572	T14601	T14602	arg2Of	material,associated
R11573	T14601	T14606	arg1Of	material,was
R11574	T14601	T14607	arg2Of	material,dephosphorylated
R11575	T14602	T14603	arg1Of	associated,with
R11576	T14605	T14603	arg2Of	beads,with
R11577	T14605	T14604	arg1Of	beads,the
R11578	T14607	T14597	arg1Of	dephosphorylated,In
R11579	T14607	T14600	arg1Of	dephosphorylated,","
R11580	T14607	T14606	arg2Of	dephosphorylated,was
R11581	T14607	T14608	arg1Of	dephosphorylated,with
R11582	T14610	T14608	arg2Of	phosphatase,with
R11583	T14610	T14609	arg1Of	phosphatase,lambda
R11584	T14610	T14611	arg1Of	phosphatase,(
R11585	T14610	T14617	arg1Of	phosphatase,per
R11586	T14614	T14611	arg2Of	BioLabs,(
R11587	T14614	T14612	arg1Of	BioLabs,New
R11588	T14614	T14613	arg1Of	BioLabs,England
R11589	T14615	T14611	arg3Of	),(
R11590	T14617	T14616	arg1Of	per,as
R11591	T14618	T14619	arg2Of	manufacturer,'s
R11592	T14620	T14617	arg2Of	instructions,per
R11593	T14620	T14619	arg1Of	instructions,'s
R11594	T14621	T14622	arg1Of	Beads,were
R11595	T14621	T14623	arg2Of	Beads,resuspended
R11596	T14621	T14630	arg2Of	Beads,heated
R11597	T14623	T14624	arg1Of	resuspended,in
R11598	T14623	T14629	arg1Of	resuspended,and
R11599	T14628	T14624	arg2Of	buffer,in
R11600	T14628	T14625	arg1Of	buffer,2X
R11601	T14628	T14626	arg1Of	buffer,SDS-PAGE
R11602	T14628	T14627	arg1Of	buffer,sample
R11603	T14629	T14622	arg2Of	and,were
R11604	T14630	T14629	arg2Of	heated,and
R11605	T14630	T14631	arg1Of	heated,for
R11606	T14633	T14632	arg1Of	minutes,5
R11607	T14633	T14634	arg1Of	minutes,at
R11608	T14633	T14641	arg1Of	minutes,was
R11609	T14633	T14642	arg2Of	minutes,fractionated
R11610	T14633	T14646	arg2Of	minutes,transferred
R11611	T14636	T14634	arg2Of	Material,at
R11612	T14636	T14635	arg1Of	Material,95°C.
R11613	T14636	T14637	arg2Of	Material,eluted
R11614	T14637	T14638	arg1Of	eluted,from
R11615	T14640	T14638	arg2Of	beads,from
R11616	T14640	T14639	arg1Of	beads,the
R11617	T14642	T14645	arg1Of	fractionated,and
R11618	T14644	T14642	arg1Of	SDS-PAGE,fractionated
R11619	T14644	T14643	arg2Of	SDS-PAGE,by
R11620	T14645	T14622	modOf	and,were
R11621	T14645	T14641	arg2Of	and,was
R11622	T14646	T14645	arg2Of	transferred,and
R11623	T14646	T14647	arg1Of	transferred,to
R11624	T14646	T14650	arg1Of	transferred,for
R11625	T14649	T14647	arg2Of	membranes,to
R11626	T14649	T14648	arg1Of	membranes,PVDF
R11627	T14652	T14650	arg2Of	blotting,for
R11628	T14652	T14651	arg1Of	blotting,Western
R11629	T14655	T14653	arg2Of	experiments,In
R11630	T14655	T14654	arg1Of	experiments,some
R11631	T14657	T14658	arg1Of	cells,were
R11632	T14657	T14659	arg2Of	cells,cultured
R11633	T14659	T14653	arg1Of	cultured,In
R11634	T14659	T14656	arg1Of	cultured,","
R11635	T14659	T14658	arg2Of	cultured,were
R11636	T14659	T14660	arg1Of	cultured,for
R11637	T14662	T14660	arg2Of	hrs,for
R11638	T14662	T14661	arg1Of	hrs,6
R11639	T14662	T14663	arg1Of	hrs,with
R11640	T14668	T14664	arg1Of	peptide,25
R11641	T14668	T14665	arg1Of	peptide,µM
R11642	T14668	T14666	arg1Of	peptide,antennapedia-linked
R11643	T14668	T14667	arg1Of	peptide,SMAC-N7
R11644	T14668	T14669	arg1Of	peptide,(
R11645	T14668	T14672	arg1Of	peptide,or
R11646	T14670	T14669	arg2Of	Calbiochem,(
R11647	T14671	T14669	arg3Of	),(
R11648	T14672	T14663	arg2Of	or,with
R11649	T14675	T14672	arg2Of	volume,or
R11650	T14675	T14673	arg1Of	volume,an
R11651	T14675	T14674	arg1Of	volume,appropriate
R11652	T14675	T14676	arg1Of	volume,of
R11653	T14678	T14676	arg2Of	solvent,of
R11654	T14678	T14677	arg1Of	solvent,the
R11655	T14678	T14679	arg2Of	solvent,used
R11656	T14679	T14680	arg1Of	used,for
R11657	T14682	T14680	arg2Of	peptide,for
R11658	T14682	T14681	arg1Of	peptide,the
R11659	T14682	T14683	arg1Of	peptide,(
R11660	T14684	T14683	arg2Of	DMSO,(
R11661	T14685	T14683	arg3Of	),(
R11662	T14687	T14686	arg2Of	incubation,After
R11663	T14689	T14690	arg1Of	cells,(
R11664	T14689	T14697	arg1Of	cells,were
R11665	T14689	T14698	arg2Of	cells,stimulated
R11666	T14691	T14690	arg2Of	in,(
R11667	T14692	T14693	arg1Of	peptide-,or
R11668	T14694	T14693	arg2Of	DMSO-containing,or
R11669	T14695	T14691	arg2Of	medium,in
R11670	T14695	T14692	arg1Of	medium,peptide-
R11671	T14695	T14694	arg1Of	medium,DMSO-containing
R11672	T14696	T14690	arg3Of	),(
R11673	T14698	T14686	arg1Of	stimulated,After
R11674	T14698	T14688	arg1Of	stimulated,","
R11675	T14698	T14697	arg2Of	stimulated,were
R11676	T14698	T14699	arg1Of	stimulated,with
R11677	T14698	T14702	arg1Of	stimulated,as
R11678	T14701	T14699	arg2Of	beads,with
R11679	T14701	T14700	arg1Of	beads,antibody-coated
R11680	T14703	T14702	arg2Of	outlined,as
R11681	T14703	T14704	arg1Of	outlined,above

2_test

Id	Subject	Object	Predicate	Lexical cue
21829693-310843-89653795	94-96	310843	denotes	26
21829693-6605987-89653796	100-102	6605987	denotes	27
21829693-8757608-89653797	261-263	8757608	denotes	28
21829693-8939935-89653798	319-321	8939935	denotes	29
21829693-20614026-89653799	616-617	20614026	denotes	6
21829693-20614026-89653800	879-880	20614026	denotes	6
21829693-12958312-89653801	1032-1033	12958312	denotes	7
21829693-17878362-89653802	1037-1038	17878362	denotes	8
21829693-12958312-89653803	1618-1619	12958312	denotes	7
21829693-12958312-89653804	1810-1811	12958312	denotes	7
21829693-10514006-89653805	2619-2621	10514006	denotes	31
21829693-8764092-89653806	3032-3034	8764092	denotes	32
21829693-8757608-89653807	3716-3718	8757608	denotes	28
21829693-20080759-89653808	3968-3970	20080759	denotes	33
21829693-20614026-89653809	5525-5526	20614026	denotes	6
T69278	94-96	310843	denotes	26
T93730	100-102	6605987	denotes	27
T91735	261-263	8757608	denotes	28
T5055	319-321	8939935	denotes	29
T47419	616-617	20614026	denotes	6
T73855	879-880	20614026	denotes	6
T56054	1032-1033	12958312	denotes	7
T48514	1037-1038	17878362	denotes	8
T27828	1618-1619	12958312	denotes	7
T21517	1810-1811	12958312	denotes	7
T34887	2619-2621	10514006	denotes	31
T49415	3032-3034	8764092	denotes	32
T36639	3716-3718	8757608	denotes	28
T37515	3968-3970	20080759	denotes	33
T68192	5525-5526	20614026	denotes	6

bionlp-st-ge-2016-spacy-parsed

Id	Subject	Object	Predicate	Lexical cue
T11924	23-27	NN	denotes	Cell
T11925	28-33	NNS	denotes	lines
T11926	34-37	DT	denotes	The
T11927	38-43	NN	denotes	mouse
T11928	44-45	NNP	denotes	B
T11929	46-50	NN	denotes	cell
T11930	51-55	NN	denotes	line
T11931	56-59	NNP	denotes	A20
T11932	59-61	CD	denotes	.2
T11933	61-62	NNP	denotes	J
T11934	63-66	VBZ	denotes	has
T11935	67-71	VBN	denotes	been
T11936	72-82	RB	denotes	previously
T11937	83-92	VBN	denotes	described
T11938	93-94	NNP	denotes	[
T11939	94-96	CD	denotes	26
T11940	96-97	NNP	denotes	]
T11941	97-98	,	denotes	,
T11942	99-100	NNP	denotes	[
T11943	100-102	CD	denotes	27
T11944	102-103	NNP	denotes	]
T11945	103-104	.	denotes	.
T11946	105-110	NNS	denotes	Cells
T11947	111-115	VBD	denotes	were
T11948	116-121	VBN	denotes	grown
T11949	122-124	IN	denotes	in
T11950	125-129	NNP	denotes	RPMI
T11951	130-134	CD	denotes	1640
T11952	134-135	,	denotes	,
T11953	136-138	CD	denotes	10
T11954	138-139	NN	denotes	%
T11955	140-143	NNP	denotes	FCS
T11956	143-144	,	denotes	,
T11957	145-147	CD	denotes	10
T11958	148-150	NNP	denotes	µM
T11959	151-155	NNP	denotes	2-ME
T11960	155-156	,	denotes	,
T11961	157-158	CD	denotes	2
T11962	159-161	NNP	denotes	mM
T11963	162-173	NNP	denotes	L-glutamine
T11964	173-174	,	denotes	,
T11965	175-178	CC	denotes	and
T11966	179-190	NNS	denotes	antibiotics
T11967	190-191	.	denotes	.
T11968	192-196	CD	denotes	HI-5
T11969	197-203	JJ	denotes	insect
T11970	204-209	NNS	denotes	cells
T11971	210-211	-LRB-	denotes	(
T11972	211-221	NNP	denotes	Invitrogen
T11973	221-222	-RRB-	denotes	)
T11974	223-233	VBG	denotes	expressing
T11975	234-239	CD	denotes	CD154
T11976	240-244	VBP	denotes	have
T11977	245-249	VBN	denotes	been
T11978	250-259	VBN	denotes	described
T11979	260-261	NNP	denotes	[
T11980	261-263	CD	denotes	28
T11981	263-264	NNP	denotes	]
T11982	264-265	.	denotes	.
T12059	267-277	NNPS	denotes	Antibodies
T12060	278-283	NNP	denotes	Mouse
T12061	284-294	JJ	denotes	anti-birch
T12062	295-303	NN	denotes	profilin
T12063	304-312	NN	denotes	antibody
T12064	313-314	-LRB-	denotes	(
T12065	314-317	NNP	denotes	4A6
T12066	318-319	NNP	denotes	[
T12067	319-321	CD	denotes	29
T12068	321-322	NNP	denotes	]
T12069	322-323	-RRB-	denotes	)
T12070	323-324	,	denotes	,
T12071	325-328	NN	denotes	rat
T12072	329-339	JJ	denotes	anti-mouse
T12073	340-344	NNP	denotes	CD40
T12074	345-346	-LRB-	denotes	(
T12075	346-350	NNP	denotes	1C10
T12076	351-352	NNP	denotes	[
T12077	352-354	CD	denotes	30
T12078	354-355	NNP	denotes	]
T12079	355-356	-RRB-	denotes	)
T12080	357-360	CC	denotes	and
T12081	361-362	DT	denotes	a
T12082	363-366	NN	denotes	rat
T12083	367-374	NN	denotes	isotype
T12084	375-382	NN	denotes	control
T12085	383-391	NN	denotes	antibody
T12086	392-393	-LRB-	denotes	(
T12087	393-398	NNP	denotes	mAb72
T12088	398-399	,	denotes	,
T12089	400-413	NNP	denotes	Developmental
T12090	414-421	NNP	denotes	Studies
T12091	422-431	NNP	denotes	Hybridoma
T12092	432-436	NNP	denotes	Bank
T12093	436-437	,	denotes	,
T12094	438-448	NNP	denotes	University
T12095	449-451	IN	denotes	of
T12096	452-456	NNP	denotes	Iowa
T12097	456-457	,	denotes	,
T12098	458-462	NNP	denotes	Iowa
T12099	463-467	NNP	denotes	City
T12100	467-468	,	denotes	,
T12101	469-471	NNP	denotes	IA
T12102	471-472	-RRB-	denotes	)
T12103	473-477	VBD	denotes	were
T12104	478-486	VBN	denotes	isolated
T12105	487-491	IN	denotes	from
T12106	492-501	NN	denotes	hybridoma
T12107	502-514	NNS	denotes	supernatants
T12108	514-515	.	denotes	.
T12109	516-528	JJ	denotes	FITC-labeled
T12110	529-539	JJ	denotes	anti-mouse
T12111	540-544	NN	denotes	CD80
T12112	545-548	CC	denotes	and
T12113	549-551	DT	denotes	an
T12114	552-559	NN	denotes	isotype
T12115	560-567	NN	denotes	control
T12116	568-570	NNP	denotes	Ab
T12117	571-575	VBD	denotes	were
T12118	576-580	IN	denotes	from
T12119	581-593	NNS	denotes	eBiosciences
T12120	593-594	.	denotes	.
T12121	595-601	NN	denotes	Rabbit
T12122	602-611	JJ	denotes	anti-HOIP
T12123	612-614	NNP	denotes	Ab
T12124	615-616	NNP	denotes	[
T12125	616-617	CD	denotes	6
T12126	617-618	NNP	denotes	]
T12127	619-622	VBD	denotes	was
T12128	623-626	DT	denotes	the
T12129	627-631	NN	denotes	kind
T12130	632-636	NN	denotes	gift
T12131	637-639	IN	denotes	of
T12132	640-643	NNP	denotes	Dr.
T12133	644-649	NNP	denotes	Betty
T12134	650-656	NNP	denotes	Eipper
T12135	657-658	-LRB-	denotes	(
T12136	658-668	NNP	denotes	University
T12137	669-671	IN	denotes	of
T12138	672-683	NNP	denotes	Connecticut
T12139	684-690	NNP	denotes	Health
T12140	691-697	NNP	denotes	Center
T12141	697-698	,	denotes	,
T12142	699-709	NNP	denotes	Farmington
T12143	709-710	,	denotes	,
T12144	711-722	NNP	denotes	Connecticut
T12145	722-723	-RRB-	denotes	)
T12146	723-724	.	denotes	.
T12147	725-729	NNP	denotes	Goat
T12148	730-738	JJ	denotes	anti-rat
T12149	739-742	NNP	denotes	IgG
T12150	742-743	,	denotes	,
T12151	744-747	CC	denotes	and
T12152	748-759	JJ	denotes	HRP-labeled
T12153	760-769	JJ	denotes	secondary
T12154	770-773	NNS	denotes	Abs
T12155	774-778	VBD	denotes	were
T12156	779-783	IN	denotes	from
T12157	784-791	NNP	denotes	Jackson
T12158	792-806	NNP	denotes	ImmunoResearch
T12159	807-819	NNPS	denotes	Laboratories
T12160	819-820	,	denotes	,
T12161	821-825	NNP	denotes	Inc.
T12162	881-882	.	denotes	.
T12332	884-891	JJ	denotes	Somatic
T12333	892-896	NN	denotes	cell
T12334	897-901	NN	denotes	gene
T12335	902-911	VBG	denotes	targeting
T12336	912-915	DT	denotes	The
T12337	916-926	NN	denotes	generation
T12338	927-929	IN	denotes	of
T12339	930-944	JJ	denotes	HOIP-deficient
T12340	945-950	NNS	denotes	cells
T12341	951-954	VBD	denotes	was
T12342	955-967	VBN	denotes	accomplished
T12343	968-973	VBG	denotes	using
T12344	974-975	DT	denotes	a
T12345	976-986	RB	denotes	homologous
T12346	987-1000	NN	denotes	recombination
T12347	1001-1009	NN	denotes	approach
T12348	1010-1019	VBD	denotes	described
T12349	1020-1030	RB	denotes	previously
T12350	1031-1032	JJ	denotes	[
T12351	1032-1033	CD	denotes	7
T12352	1033-1034	NNP	denotes	]
T12353	1034-1035	,	denotes	,
T12354	1036-1037	NNP	denotes	[
T12355	1037-1038	CD	denotes	8
T12356	1038-1039	NNP	denotes	]
T12357	1039-1040	.	denotes	.
T12358	1041-1049	NNS	denotes	Segments
T12359	1050-1052	IN	denotes	of
T12360	1053-1058	JJ	denotes	Rnf31
T12361	1059-1063	NN	denotes	gene
T12362	1064-1072	NN	denotes	sequence
T12363	1073-1077	VBN	denotes	used
T12364	1078-1080	IN	denotes	in
T12365	1081-1084	DT	denotes	the
T12366	1085-1094	VBG	denotes	targeting
T12367	1095-1104	VB	denotes	construct
T12368	1105-1106	-LRB-	denotes	(
T12369	1106-1110	NNP	denotes	Fig.
T12370	1111-1113	NNP	denotes	1A
T12371	1113-1114	-RRB-	denotes	)
T12372	1115-1119	VBD	denotes	were
T12373	1120-1129	VBN	denotes	amplified
T12374	1130-1132	IN	denotes	by
T12375	1133-1136	NNP	denotes	PCR
T12376	1137-1141	IN	denotes	from
T12377	1142-1145	NNP	denotes	A20
T12378	1145-1147	CD	denotes	.2
T12379	1147-1148	NNP	denotes	J
T12380	1149-1156	JJ	denotes	genomic
T12381	1157-1160	NN	denotes	DNA
T12382	1160-1161	.	denotes	.
T12383	1162-1165	DT	denotes	The
T12384	1166-1181	NN	denotes	oligonucleotide
T12385	1182-1189	NNS	denotes	primers
T12386	1190-1194	VBN	denotes	used
T12387	1195-1197	TO	denotes	to
T12388	1198-1206	VB	denotes	generate
T12389	1207-1210	DT	denotes	the
T12390	1211-1212	CD	denotes	5
T12391	1212-1213	NN	denotes	′
T12392	1214-1219	NN	denotes	flank
T12393	1220-1221	-LRB-	denotes	(
T12394	1221-1225	CD	denotes	1224
T12395	1226-1228	NN	denotes	bp
T12396	1228-1229	-RRB-	denotes	)
T12397	1230-1234	VBD	denotes	were
T12398	1235-1236	CD	denotes	5
T12399	1236-1237	SYM	denotes	′
T12400	1237-1238	:	denotes	-
T12401	1238-1268	JJ	denotes	ttttctagagcggtggcttaagtgaccc-3
T12402	1268-1269	NN	denotes	′
T12403	1270-1273	CC	denotes	and
T12404	1274-1275	CD	denotes	5
T12405	1275-1276	SYM	denotes	′
T12406	1276-1277	:	denotes	-
T12407	1277-1310	JJ	denotes	tattctagatgcagcatctgagaaagcaagc-3
T12408	1310-1311	NN	denotes	′
T12409	1311-1312	.	denotes	.
T12410	1313-1316	DT	denotes	The
T12411	1317-1318	CD	denotes	3
T12412	1318-1319	NN	denotes	′
T12413	1320-1325	NN	denotes	flank
T12414	1326-1327	-LRB-	denotes	(
T12415	1327-1331	CD	denotes	6032
T12416	1332-1334	NN	denotes	bp
T12417	1334-1335	-RRB-	denotes	)
T12418	1336-1343	NNS	denotes	primers
T12419	1344-1348	VBD	denotes	were
T12420	1349-1350	CD	denotes	5
T12421	1350-1351	SYM	denotes	′
T12422	1351-1352	:	denotes	-
T12423	1352-1393	JJ	denotes	aaaaccggtgtatgcttctttacgggagaaaaatattag-3
T12424	1393-1394	NN	denotes	′
T12425	1395-1398	CC	denotes	and
T12426	1399-1400	CD	denotes	5
T12427	1400-1401	SYM	denotes	′
T12428	1401-1402	:	denotes	-
T12429	1402-1437	JJ	denotes	tataccggtatgaagccaaaggaacactgagag-3
T12430	1437-1438	NN	denotes	′
T12431	1438-1439	.	denotes	.
T12432	1440-1451	NN	denotes	Restriction
T12433	1452-1464	NN	denotes	endonuclease
T12434	1465-1470	NNS	denotes	sites
T12435	1471-1473	IN	denotes	in
T12436	1474-1477	DT	denotes	the
T12437	1478-1493	NN	denotes	oligonucleotide
T12438	1494-1501	VBZ	denotes	primers
T12439	1502-1509	VBN	denotes	allowed
T12440	1510-1519	NN	denotes	insertion
T12441	1520-1522	IN	denotes	of
T12442	1523-1526	DT	denotes	the
T12443	1527-1530	NNP	denotes	PCR
T12444	1531-1539	NNS	denotes	products
T12445	1540-1544	IN	denotes	into
T12446	1545-1548	DT	denotes	the
T12447	1549-1558	VBG	denotes	targeting
T12448	1559-1565	NN	denotes	vector
T12449	1565-1566	.	denotes	.
T12450	1567-1570	CD	denotes	A20
T12451	1570-1572	CD	denotes	.2
T12452	1572-1573	NNP	denotes	J
T12453	1574-1579	NNS	denotes	cells
T12454	1580-1584	VBD	denotes	were
T12455	1585-1596	VBN	denotes	transfected
T12456	1597-1598	-LRB-	denotes	(
T12457	1598-1600	IN	denotes	by
T12458	1601-1616	NN	denotes	electroporation
T12459	1617-1618	NN	denotes	[
T12460	1618-1619	CD	denotes	7
T12461	1619-1620	NNP	denotes	]
T12462	1620-1621	-RRB-	denotes	)
T12463	1622-1626	IN	denotes	with
T12464	1627-1630	DT	denotes	the
T12465	1631-1640	VBG	denotes	targeting
T12466	1641-1650	VB	denotes	construct
T12467	1651-1654	CC	denotes	and
T12468	1655-1664	VBN	denotes	subcloned
T12469	1665-1667	IN	denotes	in
T12470	1668-1674	NN	denotes	medium
T12471	1675-1685	VBG	denotes	containing
T12472	1686-1689	CD	denotes	600
T12473	1690-1692	JJ	denotes	µg
T12474	1692-1693	NN	denotes	/
T12475	1693-1695	NN	denotes	ml
T12476	1696-1700	NNP	denotes	G418
T12477	1701-1708	NN	denotes	sulfate
T12478	1708-1709	.	denotes	.
T12479	1710-1720	JJ	denotes	Homologous
T12480	1721-1734	NN	denotes	recombination
T12481	1735-1737	IN	denotes	in
T12482	1738-1752	JJ	denotes	G418-resistant
T12483	1753-1759	NNS	denotes	clones
T12484	1760-1763	VBD	denotes	was
T12485	1764-1772	VBN	denotes	detected
T12486	1773-1775	IN	denotes	by
T12487	1776-1779	NNP	denotes	PCR
T12488	1780-1782	IN	denotes	of
T12489	1783-1790	JJ	denotes	genomic
T12490	1791-1794	NN	denotes	DNA
T12491	1794-1795	,	denotes	,
T12492	1796-1798	IN	denotes	as
T12493	1799-1808	VBN	denotes	described
T12494	1809-1810	NN	denotes	[
T12495	1810-1811	CD	denotes	7
T12496	1811-1812	NNP	denotes	]
T12497	1812-1813	.	denotes	.
T12498	1814-1829	NNP	denotes	Oligonucleotide
T12499	1830-1837	NNS	denotes	primers
T12500	1838-1842	VBD	denotes	used
T12501	1843-1846	IN	denotes	for
T12502	1847-1856	VBG	denotes	screening
T12503	1857-1861	VBD	denotes	were
T12504	1862-1863	CD	denotes	5
T12505	1863-1864	SYM	denotes	′
T12506	1864-1865	:	denotes	-
T12507	1865-1893	JJ	denotes	cttcctgatctcagctttaccgtcac-3
T12508	1893-1894	NN	denotes	′
T12509	1895-1896	-LRB-	denotes	(
T12510	1896-1906	RB	denotes	homologous
T12511	1907-1909	TO	denotes	to
T12512	1910-1917	JJ	denotes	genomic
T12513	1918-1926	NN	denotes	sequence
T12514	1926-1927	:	denotes	;
T12515	1928-1939	JJ	denotes	approximate
T12516	1940-1948	NN	denotes	position
T12517	1949-1954	VBD	denotes	noted
T12518	1955-1957	IN	denotes	in
T12519	1958-1962	NNP	denotes	Fig.
T12520	1963-1964	CD	denotes	1
T12521	1964-1965	-RRB-	denotes	)
T12522	1966-1969	CC	denotes	and
T12523	1970-1971	CD	denotes	5
T12524	1971-1972	SYM	denotes	′
T12525	1972-1973	:	denotes	-
T12526	1973-1996	JJ	denotes	caatccatcttgttcagccat-3
T12527	1996-1997	NN	denotes	′
T12528	1998-1999	-LRB-	denotes	(
T12529	1999-2009	RB	denotes	homologous
T12530	2010-2012	TO	denotes	to
T12531	2013-2021	NN	denotes	sequence
T12532	2022-2024	IN	denotes	in
T12533	2025-2029	NNP	denotes	NeoR
T12534	2029-2030	-RRB-	denotes	)
T12535	2030-2031	.	denotes	.
T12536	2032-2038	NNS	denotes	Clones
T12537	2039-2041	IN	denotes	in
T12538	2042-2047	WDT	denotes	which
T12539	2048-2051	CD	denotes	one
T12540	2052-2056	NN	denotes	copy
T12541	2057-2059	IN	denotes	of
T12542	2060-2065	NNP	denotes	Rnf31
T12543	2066-2069	VBD	denotes	had
T12544	2070-2074	VBN	denotes	been
T12545	2075-2084	VBN	denotes	disrupted
T12546	2085-2089	VBD	denotes	were
T12547	2090-2101	RB	denotes	transiently
T12548	2102-2113	VBN	denotes	transfected
T12549	2114-2118	IN	denotes	with
T12550	2119-2121	DT	denotes	an
T12551	2122-2132	NN	denotes	expression
T12552	2133-2140	NN	denotes	plasmid
T12553	2141-2149	VBG	denotes	encoding
T12554	2150-2153	NNP	denotes	Cre
T12555	2153-2154	,	denotes	,
T12556	2155-2157	IN	denotes	in
T12557	2158-2163	NN	denotes	order
T12558	2164-2166	TO	denotes	to
T12559	2167-2173	VB	denotes	remove
T12560	2174-2178	NNP	denotes	NeoR
T12561	2178-2179	.	denotes	.
T12562	2180-2194	JJ	denotes	G418-sensitive
T12563	2195-2204	NNS	denotes	subclones
T12564	2205-2209	VBD	denotes	were
T12565	2210-2219	VBN	denotes	subjected
T12566	2220-2222	TO	denotes	to
T12567	2223-2224	DT	denotes	a
T12568	2225-2231	JJ	denotes	second
T12569	2232-2237	NN	denotes	round
T12570	2238-2240	IN	denotes	of
T12571	2241-2250	VBG	denotes	targeting
T12572	2251-2253	TO	denotes	to
T12573	2254-2261	VB	denotes	disrupt
T12574	2262-2265	DT	denotes	the
T12575	2266-2275	VBG	denotes	remaining
T12576	2276-2280	NN	denotes	copy
T12577	2281-2283	IN	denotes	of
T12578	2284-2289	NNP	denotes	Rnf31
T12579	2289-2290	.	denotes	.
T12580	2291-2305	JJ	denotes	G418-resistant
T12581	2306-2312	NNS	denotes	clones
T12582	2313-2317	VBD	denotes	were
T12583	2318-2324	VBN	denotes	tested
T12584	2325-2328	IN	denotes	for
T12585	2329-2339	RB	denotes	homologous
T12586	2340-2353	NN	denotes	recombination
T12587	2354-2356	IN	denotes	by
T12588	2357-2360	NNP	denotes	PCR
T12589	2361-2364	CC	denotes	and
T12590	2365-2369	NNP	denotes	HOIP
T12591	2370-2377	NN	denotes	protein
T12592	2378-2388	NN	denotes	expression
T12593	2389-2391	IN	denotes	by
T12594	2392-2399	JJ	denotes	Western
T12595	2400-2404	NN	denotes	blot
T12596	2404-2405	.	denotes	.
T12911	2407-2417	NNP	denotes	Retroviral
T12912	2418-2430	NN	denotes	transduction
T12913	2431-2432	DT	denotes	A
T12914	2433-2443	JJ	denotes	retroviral
T12915	2444-2450	NN	denotes	vector
T12916	2451-2452	-LRB-	denotes	(
T12917	2452-2456	NNP	denotes	pMIP
T12918	2456-2457	-RRB-	denotes	)
T12919	2458-2461	VBD	denotes	was
T12920	2462-2466	VBN	denotes	used
T12921	2467-2470	IN	denotes	for
T12922	2471-2474	DT	denotes	the
T12923	2475-2481	JJ	denotes	stable
T12924	2482-2494	NN	denotes	transduction
T12925	2495-2497	IN	denotes	of
T12926	2498-2503	NNS	denotes	cells
T12927	2504-2508	IN	denotes	with
T12928	2509-2513	NNP	denotes	HOIP
T12929	2514-2517	CC	denotes	and
T12930	2518-2522	NNP	denotes	IKKγ
T12931	2523-2527	NNP	denotes	cDNA
T12932	2528-2538	NNS	denotes	constructs
T12933	2538-2539	.	denotes	.
T12934	2540-2544	NN	denotes	pMIP
T12935	2545-2548	VBD	denotes	was
T12936	2549-2560	VBN	denotes	constructed
T12937	2561-2563	IN	denotes	by
T12938	2564-2573	VBG	denotes	replacing
T12939	2574-2577	DT	denotes	the
T12940	2578-2582	NNP	denotes	IRES
T12941	2583-2586	CC	denotes	and
T12942	2587-2590	NNP	denotes	GFP
T12943	2591-2599	VBG	denotes	encoding
T12944	2600-2609	NNS	denotes	sequences
T12945	2610-2612	IN	denotes	in
T12946	2613-2617	NNP	denotes	pMIG
T12947	2618-2619	NNP	denotes	[
T12948	2619-2621	CD	denotes	31
T12949	2621-2622	NN	denotes	]
T12950	2623-2627	IN	denotes	with
T12951	2628-2631	DT	denotes	the
T12952	2632-2636	NNP	denotes	IRES
T12953	2637-2640	CC	denotes	and
T12954	2641-2650	JJ	denotes	puromycin
T12955	2651-2661	NN	denotes	resistance
T12956	2662-2666	NN	denotes	gene
T12957	2667-2671	IN	denotes	from
T12958	2672-2682	NNP	denotes	pIRESpuro2
T12959	2683-2684	-LRB-	denotes	(
T12960	2684-2692	NNP	denotes	Clontech
T12961	2692-2693	-RRB-	denotes	)
T12962	2693-2694	.	denotes	.
T12963	2695-2706	JJ	denotes	FLAG-tagged
T12964	2707-2712	NN	denotes	mouse
T12965	2713-2717	NNP	denotes	HOIP
T12966	2718-2722	NNP	denotes	cDNA
T12967	2723-2726	VBD	denotes	was
T12968	2727-2735	VBN	denotes	inserted
T12969	2736-2740	IN	denotes	into
T12970	2741-2744	DT	denotes	the
T12971	2745-2753	JJ	denotes	multiple
T12972	2754-2761	NN	denotes	cloning
T12973	2762-2766	NN	denotes	site
T12974	2767-2769	IN	denotes	of
T12975	2770-2774	NNP	denotes	pMIP
T12976	2774-2775	.	denotes	.
T12977	2776-2777	DT	denotes	A
T12978	2778-2782	NN	denotes	pMIP
T12979	2783-2792	VBP	denotes	construct
T12980	2793-2801	VBG	denotes	encoding
T12981	2802-2807	NN	denotes	mouse
T12982	2808-2812	NNP	denotes	IKKγ
T12983	2813-2817	IN	denotes	with
T12984	2818-2820	DT	denotes	an
T12985	2821-2826	JJ	denotes	amino
T12986	2827-2835	NN	denotes	terminal
T12987	2836-2841	NN	denotes	birch
T12988	2842-2850	NN	denotes	profilin
T12989	2851-2858	NN	denotes	peptide
T12990	2859-2860	-LRB-	denotes	(
T12991	2860-2862	NNP	denotes	BP
T12992	2862-2863	-RRB-	denotes	)
T12993	2863-2864	:	denotes	-
T12994	2864-2867	NN	denotes	tag
T12995	2868-2871	VBD	denotes	was
T12996	2872-2876	RB	denotes	also
T12997	2877-2885	VBN	denotes	prepared
T12998	2885-2886	.	denotes	.
T12999	2887-2897	NNP	denotes	Retroviral
T13000	2898-2907	NNS	denotes	particles
T13001	2908-2912	VBD	denotes	were
T13002	2913-2922	VBN	denotes	generated
T13003	2923-2925	IN	denotes	by
T13004	2926-2935	JJ	denotes	transient
T13005	2936-2948	NN	denotes	transfection
T13006	2949-2951	IN	denotes	of
T13007	2952-2956	JJ	denotes	293T
T13008	2957-2962	NNS	denotes	cells
T13009	2963-2967	IN	denotes	with
T13010	2968-2972	NNP	denotes	pMIP
T13011	2972-2973	,	denotes	,
T13012	2974-2983	NNP	denotes	pMIP-HOIP
T13013	2983-2984	,	denotes	,
T13014	2985-2987	CC	denotes	or
T13015	2988-2997	NNP	denotes	pMIP-IKKγ
T13016	2998-3001	CC	denotes	and
T13017	3002-3005	DT	denotes	the
T13018	3006-3015	NN	denotes	packaging
T13019	3016-3022	NN	denotes	vector
T13020	3023-3030	NNP	denotes	pCL-Eco
T13021	3031-3032	NNP	denotes	[
T13022	3032-3034	CD	denotes	32
T13023	3034-3035	NNP	denotes	]
T13024	3035-3036	.	denotes	.
T13025	3037-3040	CD	denotes	Two
T13026	3041-3045	NNS	denotes	days
T13027	3046-3051	IN	denotes	after
T13028	3052-3064	NN	denotes	transfection
T13029	3064-3065	,	denotes	,
T13030	3066-3073	NN	denotes	culture
T13031	3074-3086	NNS	denotes	supernatants
T13032	3087-3091	VBD	denotes	were
T13033	3092-3101	VBN	denotes	harvested
T13034	3102-3105	CC	denotes	and
T13035	3106-3114	VBN	denotes	filtered
T13036	3114-3115	.	denotes	.
T13037	3116-3119	CD	denotes	A20
T13038	3119-3121	CD	denotes	.2
T13039	3121-3122	NNP	denotes	J
T13040	3123-3125	CC	denotes	or
T13041	3126-3140	JJ	denotes	HOIP-deficient
T13042	3141-3146	NNS	denotes	cells
T13043	3147-3151	VBD	denotes	were
T13044	3152-3157	VBN	denotes	added
T13045	3158-3160	TO	denotes	to
T13046	3161-3168	JJ	denotes	24-well
T13047	3169-3175	NNS	denotes	plates
T13048	3176-3177	-LRB-	denotes	(
T13049	3177-3180	CD	denotes	2.5
T13050	3180-3181	CD	denotes	×
T13051	3181-3184	CD	denotes	105
T13052	3185-3195	NN	denotes	cells/well
T13053	3195-3196	-RRB-	denotes	)
T13054	3197-3201	IN	denotes	with
T13055	3202-3203	CD	denotes	1
T13056	3204-3206	NN	denotes	ml
T13057	3207-3223	JJ	denotes	virus-containing
T13058	3224-3235	NN	denotes	supernatant
T13059	3236-3239	CC	denotes	and
T13060	3240-3241	CD	denotes	8
T13061	3242-3244	NN	denotes	µg
T13062	3244-3245	NN	denotes	/
T13063	3245-3247	NN	denotes	ml
T13064	3248-3257	NN	denotes	polybrene
T13065	3257-3258	.	denotes	.
T13066	3259-3265	NNS	denotes	Plates
T13067	3266-3270	VBD	denotes	were
T13068	3271-3282	VBN	denotes	centrifuged
T13069	3283-3285	IN	denotes	at
T13070	3286-3289	CD	denotes	500
T13071	3290-3291	SYM	denotes	x
T13072	3292-3293	FW	denotes	g
T13073	3294-3297	IN	denotes	for
T13074	3298-3299	CD	denotes	2
T13075	3300-3303	NNS	denotes	hrs
T13076	3304-3306	IN	denotes	at
T13077	3307-3311	NN	denotes	room
T13078	3312-3323	NN	denotes	temperature
T13079	3323-3324	.	denotes	.
T13080	3325-3330	NNS	denotes	Cells
T13081	3331-3335	VBD	denotes	were
T13082	3336-3344	VBN	denotes	cultured
T13083	3345-3347	IN	denotes	in
T13084	3348-3349	NNP	denotes	B
T13085	3350-3354	NN	denotes	cell
T13086	3355-3362	NN	denotes	culture
T13087	3363-3369	NN	denotes	medium
T13088	3370-3373	IN	denotes	for
T13089	3374-3376	CD	denotes	48
T13090	3377-3380	NNS	denotes	hrs
T13091	3380-3381	,	denotes	,
T13092	3382-3387	IN	denotes	after
T13093	3388-3393	WDT	denotes	which
T13094	3394-3397	DT	denotes	the
T13095	3398-3408	VBN	denotes	transduced
T13096	3409-3414	NNS	denotes	cells
T13097	3415-3419	VBD	denotes	were
T13098	3420-3428	VBN	denotes	selected
T13099	3429-3431	IN	denotes	in
T13100	3432-3433	CD	denotes	3
T13101	3434-3436	NN	denotes	µg
T13102	3436-3437	NN	denotes	/
T13103	3437-3439	NN	denotes	ml
T13104	3440-3449	NN	denotes	puromycin
T13105	3449-3450	.	denotes	.
T13347	3452-3456	CD	denotes	CD80
T13348	3457-3469	NN	denotes	upregulation
T13349	3470-3473	CC	denotes	and
T13350	3474-3478	NN	denotes	flow
T13351	3479-3488	NN	denotes	cytometry
T13352	3489-3494	NNP	denotes	Cells
T13353	3495-3496	-LRB-	denotes	(
T13354	3496-3497	CD	denotes	5
T13355	3497-3498	CD	denotes	×
T13356	3498-3501	CD	denotes	104
T13357	3502-3504	IN	denotes	in
T13358	3505-3506	CD	denotes	2
T13359	3507-3509	NN	denotes	ml
T13360	3509-3510	-RRB-	denotes	)
T13361	3511-3515	VBD	denotes	were
T13362	3516-3524	VBN	denotes	cultured
T13363	3525-3527	IN	denotes	in
T13364	3528-3535	JJ	denotes	24-well
T13365	3536-3542	NNS	denotes	plates
T13366	3543-3546	IN	denotes	for
T13367	3547-3548	CD	denotes	3
T13368	3549-3553	NNS	denotes	days
T13369	3554-3558	IN	denotes	with
T13370	3559-3560	CD	denotes	5
T13371	3561-3563	NN	denotes	µg
T13372	3563-3564	NN	denotes	/
T13373	3564-3566	NN	denotes	ml
T13374	3567-3576	JJ	denotes	anti-CD40
T13375	3577-3579	CC	denotes	or
T13376	3580-3582	DT	denotes	an
T13377	3583-3590	NN	denotes	isotype
T13378	3591-3598	NN	denotes	control
T13379	3599-3607	NN	denotes	antibody
T13380	3607-3608	.	denotes	.
T13381	3609-3614	NNS	denotes	Cells
T13382	3615-3619	VBD	denotes	were
T13383	3620-3627	VBN	denotes	stained
T13384	3628-3631	IN	denotes	for
T13385	3632-3636	NN	denotes	flow
T13386	3637-3646	NN	denotes	cytometry
T13387	3647-3651	IN	denotes	with
T13388	3652-3666	NN	denotes	FITC-anti-CD80
T13389	3667-3669	CC	denotes	or
T13390	3670-3672	DT	denotes	an
T13391	3673-3684	JJ	denotes	appropriate
T13392	3685-3692	NN	denotes	control
T13393	3693-3701	NN	denotes	antibody
T13394	3702-3704	IN	denotes	as
T13395	3705-3714	VBN	denotes	described
T13396	3715-3716	NNP	denotes	[
T13397	3716-3718	CD	denotes	28
T13398	3718-3719	NNP	denotes	]
T13399	3719-3720	.	denotes	.
T13400	3721-3725	NNP	denotes	Data
T13401	3726-3734	VBD	denotes	acquired
T13402	3735-3739	IN	denotes	with
T13403	3740-3741	DT	denotes	a
T13404	3742-3749	JJ	denotes	FACScan
T13405	3750-3754	NN	denotes	flow
T13406	3755-3764	NN	denotes	cytometer
T13407	3765-3766	-LRB-	denotes	(
T13408	3766-3768	NNP	denotes	BD
T13409	3769-3780	NNPS	denotes	Biosciences
T13410	3780-3781	-RRB-	denotes	)
T13411	3782-3786	VBD	denotes	were
T13412	3787-3795	VBN	denotes	analyzed
T13413	3796-3800	IN	denotes	with
T13414	3801-3807	NNP	denotes	WinMDI
T13415	3808-3811	CD	denotes	2.8
T13416	3812-3813	-LRB-	denotes	(
T13417	3813-3820	NNP	denotes	Scripps
T13418	3821-3829	NNP	denotes	Research
T13419	3830-3839	NNP	denotes	Institute
T13420	3839-3840	,	denotes	,
T13421	3841-3844	NNP	denotes	San
T13422	3845-3850	NNP	denotes	Diego
T13423	3850-3851	,	denotes	,
T13424	3852-3854	NNP	denotes	CA
T13425	3854-3855	-RRB-	denotes	)
T13426	3855-3856	.	denotes	.
T13569	3858-3861	NNP	denotes	GLε
T13570	3862-3872	NN	denotes	transcript
T13571	3873-3878	NN	denotes	assay
T13572	3879-3882	DT	denotes	The
T13573	3883-3897	JJ	denotes	CD40-simulated
T13574	3898-3908	NN	denotes	activation
T13575	3909-3911	IN	denotes	of
T13576	3912-3915	NNP	denotes	GLε
T13577	3916-3929	NN	denotes	transcription
T13578	3930-3933	VBD	denotes	was
T13579	3934-3943	VBN	denotes	evaluated
T13580	3944-3946	IN	denotes	as
T13581	3947-3957	RB	denotes	previously
T13582	3958-3966	VBN	denotes	reported
T13583	3967-3968	NNP	denotes	[
T13584	3968-3970	CD	denotes	33
T13585	3970-3971	NNP	denotes	]
T13586	3971-3972	.	denotes	.
T13587	3973-3980	RB	denotes	Briefly
T13588	3980-3981	,	denotes	,
T13589	3982-3983	CD	denotes	1
T13590	3983-3984	CD	denotes	×
T13591	3984-3987	CD	denotes	106
T13592	3988-3993	NNS	denotes	cells
T13593	3994-3998	VBD	denotes	were
T13594	3999-4009	VBN	denotes	stimulated
T13595	4010-4019	JJ	denotes	overnight
T13596	4020-4024	IN	denotes	with
T13597	4025-4034	JJ	denotes	anti-CD40
T13598	4035-4043	NN	denotes	antibody
T13599	4044-4045	-LRB-	denotes	(
T13600	4045-4047	CD	denotes	10
T13601	4048-4050	NN	denotes	µg
T13602	4050-4051	NN	denotes	/
T13603	4051-4053	NN	denotes	ml
T13604	4053-4054	-RRB-	denotes	)
T13605	4055-4057	CC	denotes	or
T13606	4058-4060	DT	denotes	an
T13607	4061-4068	NN	denotes	isotype
T13608	4069-4076	NN	denotes	control
T13609	4077-4085	NN	denotes	antibody
T13610	4085-4086	,	denotes	,
T13611	4087-4091	IN	denotes	with
T13612	4092-4094	CC	denotes	or
T13613	4095-4102	IN	denotes	without
T13614	4103-4106	CD	denotes	500
T13615	4107-4111	NNP	denotes	U/ml
T13616	4112-4117	NN	denotes	mouse
T13617	4118-4122	NN	denotes	IL-4
T13618	4123-4124	-LRB-	denotes	(
T13619	4124-4126	NNP	denotes	BD
T13620	4127-4138	NNPS	denotes	Biosciences
T13621	4138-4139	-RRB-	denotes	)
T13622	4139-4140	.	denotes	.
T13623	4141-4144	NNP	denotes	RNA
T13624	4145-4148	VBD	denotes	was
T13625	4149-4157	VBN	denotes	isolated
T13626	4158-4163	VBG	denotes	using
T13627	4164-4170	NNP	denotes	Trizol
T13628	4171-4172	-LRB-	denotes	(
T13629	4172-4182	NNP	denotes	Invitrogen
T13630	4182-4183	-RRB-	denotes	)
T13631	4183-4184	,	denotes	,
T13632	4185-4188	CC	denotes	and
T13633	4189-4208	JJ	denotes	reverse-transcribed
T13634	4209-4210	-LRB-	denotes	(
T13635	4210-4221	NNP	denotes	Superscript
T13636	4222-4225	NNP	denotes	III
T13637	4226-4229	NN	denotes	kit
T13638	4229-4230	,	denotes	,
T13639	4231-4241	NNP	denotes	Invitrogen
T13640	4241-4242	-RRB-	denotes	)
T13641	4242-4243	.	denotes	.
T13642	4244-4256	JJ	denotes	Quantitative
T13643	4257-4260	NNP	denotes	PCR
T13644	4261-4264	IN	denotes	for
T13645	4265-4268	NNP	denotes	GLε
T13646	4269-4272	CC	denotes	and
T13647	4273-4278	NNP	denotes	Hprt1
T13648	4279-4282	VBD	denotes	was
T13649	4283-4292	VBN	denotes	performed
T13650	4293-4298	VBG	denotes	using
T13651	4299-4303	NNP	denotes	SYBR
T13652	4304-4309	NNP	denotes	GREEN
T13653	4310-4316	NN	denotes	master
T13654	4317-4320	NN	denotes	mix
T13655	4321-4322	-LRB-	denotes	(
T13656	4322-4329	NNP	denotes	Applied
T13657	4330-4340	NNPS	denotes	Biosystems
T13658	4340-4341	-RRB-	denotes	)
T13659	4341-4342	,	denotes	,
T13660	4343-4346	CC	denotes	and
T13661	4347-4349	DT	denotes	an
T13662	4350-4357	NNP	denotes	Applied
T13663	4358-4368	NNPS	denotes	Biosystems
T13664	4369-4375	NNP	denotes	7900HT
T13665	4376-4380	NNP	denotes	Fast
T13666	4381-4390	NNP	denotes	Real-Time
T13667	4391-4394	NNP	denotes	PCR
T13668	4395-4405	NN	denotes	instrument
T13669	4405-4406	.	denotes	.
T13670	4407-4417	NN	denotes	Expression
T13671	4418-4420	IN	denotes	of
T13672	4421-4424	NNP	denotes	GLε
T13673	4425-4427	IN	denotes	in
T13674	4428-4432	DT	denotes	each
T13675	4433-4439	NN	denotes	sample
T13676	4440-4443	VBD	denotes	was
T13677	4444-4454	VBN	denotes	normalized
T13678	4455-4457	TO	denotes	to
T13679	4458-4461	DT	denotes	the
T13680	4462-4472	NN	denotes	expression
T13681	4473-4475	IN	denotes	of
T13682	4476-4481	NNP	denotes	Hprt1
T13683	4481-4482	.	denotes	.
T13876	4484-4489	NN	denotes	NF-κB
T13877	4490-4493	CC	denotes	and
T13878	4494-4497	NNP	denotes	JNK
T13879	4498-4508	NN	denotes	activation
T13880	4509-4515	NN	denotes	assays
T13881	4516-4519	IN	denotes	For
T13882	4520-4530	NN	denotes	activation
T13883	4531-4533	IN	denotes	of
T13884	4534-4538	NNP	denotes	CD40
T13885	4539-4548	VBG	denotes	signaling
T13886	4548-4549	,	denotes	,
T13887	4550-4551	CD	denotes	5
T13888	4551-4552	NN	denotes	×
T13889	4552-4555	CD	denotes	104
T13890	4556-4559	CD	denotes	HI5
T13891	4560-4566	NN	denotes	insect
T13892	4567-4572	NNS	denotes	cells
T13893	4573-4575	CC	denotes	or
T13894	4576-4579	CD	denotes	HI5
T13895	4580-4586	JJ	denotes	insect
T13896	4587-4592	NNS	denotes	cells
T13897	4593-4603	VBG	denotes	expressing
T13898	4604-4609	NN	denotes	mouse
T13899	4610-4615	NNP	denotes	CD154
T13900	4616-4617	-LRB-	denotes	(
T13901	4617-4621	NNP	denotes	CD40
T13902	4622-4628	NN	denotes	ligand
T13903	4628-4629	-RRB-	denotes	)
T13904	4630-4634	VBD	denotes	were
T13905	4635-4640	VBN	denotes	added
T13906	4641-4643	TO	denotes	to
T13907	4644-4645	CD	denotes	1
T13908	4645-4646	CD	denotes	×
T13909	4646-4649	CD	denotes	106
T13910	4650-4651	NNP	denotes	B
T13911	4652-4657	NNS	denotes	cells
T13912	4657-4658	.	denotes	.
T13913	4659-4664	NNS	denotes	Cells
T13914	4665-4669	VBD	denotes	were
T13915	4670-4681	VBN	denotes	centrifuged
T13916	4682-4685	IN	denotes	for
T13917	4686-4687	CD	denotes	1
T13918	4688-4694	NN	denotes	minute
T13919	4695-4697	IN	denotes	at
T13920	4698-4701	CD	denotes	400
T13921	4702-4703	SYM	denotes	x
T13922	4704-4705	FW	denotes	g
T13923	4706-4707	-LRB-	denotes	(
T13924	4707-4709	TO	denotes	to
T13925	4710-4717	VB	denotes	promote
T13926	4718-4725	NN	denotes	contact
T13927	4726-4733	IN	denotes	between
T13928	4734-4740	JJ	denotes	ligand
T13929	4741-4746	NNS	denotes	cells
T13930	4747-4750	CC	denotes	and
T13931	4751-4752	NNP	denotes	B
T13932	4753-4758	NNS	denotes	cells
T13933	4758-4759	-RRB-	denotes	)
T13934	4760-4764	RB	denotes	then
T13935	4765-4774	VBD	denotes	incubated
T13936	4775-4777	IN	denotes	at
T13937	4778-4780	CD	denotes	37
T13938	4780-4781	CD	denotes	°
T13939	4781-4782	NNP	denotes	C
T13940	4783-4786	IN	denotes	for
T13941	4787-4792	NNS	denotes	times
T13942	4793-4802	VBD	denotes	indicated
T13943	4803-4805	IN	denotes	in
T13944	4806-4810	NNP	denotes	Fig.
T13945	4811-4812	CD	denotes	4
T13946	4812-4813	.	denotes	.
T13947	4814-4823	VBG	denotes	Following
T13948	4824-4835	NN	denotes	stimulation
T13949	4835-4836	,	denotes	,
T13950	4837-4842	NNS	denotes	cells
T13951	4843-4847	VBD	denotes	were
T13952	4848-4855	VBN	denotes	chilled
T13953	4856-4858	IN	denotes	on
T13954	4859-4862	NN	denotes	ice
T13955	4863-4866	IN	denotes	for
T13956	4867-4868	CD	denotes	2
T13957	4869-4876	NNS	denotes	minutes
T13958	4876-4877	.	denotes	.
T13959	4878-4882	NN	denotes	Cell
T13960	4883-4890	NNS	denotes	pellets
T13961	4891-4895	VBD	denotes	were
T13962	4896-4905	VBN	denotes	dissolved
T13963	4906-4908	IN	denotes	in
T13964	4909-4911	NNP	denotes	2X
T13965	4912-4920	NNP	denotes	SDS-PAGE
T13966	4921-4928	VBG	denotes	loading
T13967	4929-4935	NN	denotes	buffer
T13968	4935-4936	,	denotes	,
T13969	4937-4946	VBN	denotes	sonicated
T13970	4946-4947	,	denotes	,
T13971	4948-4951	CC	denotes	and
T13972	4952-4958	VBN	denotes	heated
T13973	4959-4962	IN	denotes	for
T13974	4963-4964	CD	denotes	5
T13975	4965-4972	NNS	denotes	minutes
T13976	4973-4975	IN	denotes	at
T13977	4976-4978	CD	denotes	95
T13978	4978-4979	CD	denotes	°
T13979	4979-4981	NNP	denotes	C.
T13980	4982-4987	NNP	denotes	Total
T13981	4988-4992	NN	denotes	cell
T13982	4993-5000	VBZ	denotes	lysates
T13983	5001-5002	-LRB-	denotes	(
T13984	5002-5003	CD	denotes	1
T13985	5003-5004	CD	denotes	×
T13986	5004-5007	CD	denotes	105
T13987	5008-5012	NN	denotes	cell
T13988	5013-5024	NNS	denotes	equivalents
T13989	5025-5028	IN	denotes	per
T13990	5029-5033	NN	denotes	lane
T13991	5033-5034	-RRB-	denotes	)
T13992	5035-5039	VBD	denotes	were
T13993	5040-5052	VBN	denotes	fractionated
T13994	5053-5055	IN	denotes	by
T13995	5056-5064	NNP	denotes	SDS-PAGE
T13996	5065-5068	CC	denotes	and
T13997	5069-5076	VBD	denotes	blotted
T13998	5077-5079	TO	denotes	to
T13999	5080-5094	VB	denotes	polyvinylidine
T14000	5095-5103	NN	denotes	fluoride
T14001	5104-5105	-LRB-	denotes	(
T14002	5105-5109	NNP	denotes	PVDF
T14003	5109-5110	-RRB-	denotes	)
T14004	5111-5120	NNS	denotes	membranes
T14005	5120-5121	.	denotes	.
T14006	5122-5131	NNS	denotes	Membranes
T14007	5132-5136	VBD	denotes	were
T14008	5137-5143	VBN	denotes	probed
T14009	5144-5148	IN	denotes	with
T14010	5149-5159	NNS	denotes	antibodies
T14011	5160-5162	IN	denotes	as
T14012	5163-5172	VBN	denotes	indicated
T14013	5173-5175	IN	denotes	in
T14014	5176-5180	NNP	denotes	Fig.
T14015	5181-5182	CD	denotes	4
T14016	5182-5183	.	denotes	.
T14017	5184-5200	JJ	denotes	Chemiluminescent
T14018	5201-5210	NN	denotes	detection
T14019	5211-5212	-LRB-	denotes	(
T14020	5212-5218	NNP	denotes	Pierce
T14021	5219-5232	NNP	denotes	Biotechnology
T14022	5232-5233	-RRB-	denotes	)
T14023	5234-5237	VBD	denotes	was
T14024	5238-5242	VBN	denotes	used
T14025	5243-5246	IN	denotes	for
T14026	5247-5250	DT	denotes	the
T14027	5251-5264	NN	denotes	visualization
T14028	5265-5267	IN	denotes	of
T14029	5268-5273	NNS	denotes	bands
T14030	5274-5276	IN	denotes	on
T14031	5277-5284	JJ	denotes	Western
T14032	5285-5290	NNS	denotes	blots
T14033	5290-5291	.	denotes	.
T14034	5292-5298	NNS	denotes	Images
T14035	5299-5301	IN	denotes	of
T14036	5302-5307	NNS	denotes	blots
T14037	5308-5312	VBD	denotes	were
T14038	5313-5321	VBN	denotes	recorded
T14039	5322-5326	IN	denotes	with
T14040	5327-5328	DT	denotes	a
T14041	5329-5338	JJ	denotes	low-light
T14042	5339-5346	NN	denotes	imaging
T14043	5347-5353	NN	denotes	system
T14044	5354-5355	-LRB-	denotes	(
T14045	5355-5362	CD	denotes	LAS4000
T14046	5362-5363	,	denotes	,
T14047	5364-5368	NNP	denotes	Fuji
T14048	5369-5376	NNP	denotes	Medical
T14049	5377-5384	NNPS	denotes	Systems
T14050	5384-5385	-RRB-	denotes	)
T14051	5386-5389	CC	denotes	and
T14052	5390-5392	IN	denotes	on
T14053	5393-5398	NN	denotes	X-ray
T14054	5399-5403	NN	denotes	film
T14055	5403-5404	.	denotes	.
T14284	5406-5426	NNP	denotes	Immunoprecipitations
T14285	5427-5446	NNP	denotes	Immunoprecipitation
T14286	5447-5449	IN	denotes	of
T14287	5450-5454	NNP	denotes	CD40
T14288	5455-5458	VBD	denotes	was
T14289	5459-5468	VBN	denotes	performed
T14290	5469-5471	IN	denotes	by
T14291	5472-5481	VBN	denotes	activated
T14292	5482-5490	NN	denotes	receptor
T14293	5491-5498	NN	denotes	capture
T14294	5498-5499	,	denotes	,
T14295	5500-5502	IN	denotes	as
T14296	5503-5513	RB	denotes	previously
T14297	5514-5523	VBN	denotes	described
T14298	5524-5525	NNP	denotes	[
T14299	5525-5526	CD	denotes	6
T14300	5526-5527	NNP	denotes	]
T14301	5527-5528	.	denotes	.
T14302	5529-5536	RB	denotes	Briefly
T14303	5536-5537	,	denotes	,
T14304	5538-5539	CD	denotes	3
T14305	5539-5540	NN	denotes	×
T14306	5540-5543	CD	denotes	107
T14307	5544-5549	NNS	denotes	cells
T14308	5550-5551	-LRB-	denotes	(
T14309	5551-5554	CD	denotes	A20
T14310	5554-5556	CD	denotes	.2
T14311	5556-5557	NNP	denotes	J
T14312	5558-5561	CC	denotes	and
T14313	5562-5573	NNS	denotes	derivatives
T14314	5573-5574	-RRB-	denotes	)
T14315	5575-5579	VBD	denotes	were
T14316	5580-5589	VBN	denotes	incubated
T14317	5590-5593	IN	denotes	for
T14318	5594-5596	CD	denotes	60
T14319	5597-5604	NNS	denotes	minutes
T14320	5605-5607	IN	denotes	at
T14321	5608-5612	NN	denotes	room
T14322	5613-5624	NN	denotes	temperature
T14323	5625-5629	IN	denotes	with
T14324	5630-5632	CD	denotes	10
T14325	5633-5635	NN	denotes	µl
T14326	5636-5644	JJ	denotes	magnetic
T14327	5645-5652	NN	denotes	protein
T14328	5653-5654	NNP	denotes	G
T14329	5655-5660	NNS	denotes	beads
T14330	5661-5662	-LRB-	denotes	(
T14331	5662-5667	NNP	denotes	Dynal
T14332	5667-5668	-RRB-	denotes	)
T14333	5669-5679	JJ	denotes	pre-coated
T14334	5680-5684	IN	denotes	with
T14335	5685-5687	CD	denotes	10
T14336	5688-5690	NN	denotes	µg
T14337	5691-5695	NN	denotes	goat
T14338	5696-5704	JJ	denotes	anti-rat
T14339	5705-5708	NNP	denotes	IgG
T14340	5709-5710	-LRB-	denotes	(
T14341	5710-5717	NNP	denotes	Jackson
T14342	5717-5718	-RRB-	denotes	)
T14343	5718-5719	,	denotes	,
T14344	5720-5723	CC	denotes	and
T14345	5724-5726	CD	denotes	10
T14346	5727-5729	NN	denotes	µg
T14347	5730-5739	NN	denotes	anti-CD40
T14348	5740-5741	-LRB-	denotes	(
T14349	5741-5745	NNP	denotes	1C10
T14350	5745-5746	-RRB-	denotes	)
T14351	5747-5749	CC	denotes	or
T14352	5750-5752	DT	denotes	an
T14353	5753-5760	NN	denotes	isotype
T14354	5761-5768	NN	denotes	control
T14355	5769-5777	NN	denotes	antibody
T14356	5778-5779	-LRB-	denotes	(
T14357	5779-5784	NNP	denotes	mAb72
T14358	5784-5785	-RRB-	denotes	)
T14359	5785-5786	.	denotes	.
T14360	5787-5798	NNS	denotes	Cells/beads
T14361	5799-5803	VBD	denotes	were
T14362	5804-5808	RB	denotes	then
T14363	5809-5817	VBN	denotes	pelleted
T14364	5818-5820	IN	denotes	by
T14365	5821-5835	NN	denotes	centrifugation
T14366	5836-5839	CC	denotes	and
T14367	5840-5845	VBN	denotes	lysed
T14368	5846-5848	IN	denotes	in
T14369	5849-5855	NN	denotes	buffer
T14370	5856-5866	VBG	denotes	containing
T14371	5867-5868	CD	denotes	1
T14372	5868-5869	NN	denotes	%
T14373	5870-5876	NNP	denotes	Triton
T14374	5877-5881	NNP	denotes	X100
T14375	5881-5882	.	denotes	.
T14376	5883-5888	NNS	denotes	Beads
T14377	5889-5893	VBD	denotes	were
T14378	5894-5900	VBN	denotes	washed
T14379	5901-5905	IN	denotes	with
T14380	5906-5911	NN	denotes	lysis
T14381	5912-5918	NN	denotes	buffer
T14382	5919-5921	TO	denotes	to
T14383	5922-5928	VB	denotes	remove
T14384	5929-5936	JJ	denotes	unbound
T14385	5937-5945	NN	denotes	material
T14386	5945-5946	.	denotes	.
T14387	5947-5949	IN	denotes	In
T14388	5950-5954	DT	denotes	some
T14389	5955-5966	NNS	denotes	experiments
T14390	5966-5967	,	denotes	,
T14391	5968-5976	NN	denotes	material
T14392	5977-5987	VBN	denotes	associated
T14393	5988-5992	IN	denotes	with
T14394	5993-5996	DT	denotes	the
T14395	5997-6002	NNS	denotes	beads
T14396	6003-6006	VBD	denotes	was
T14397	6007-6023	VBN	denotes	dephosphorylated
T14398	6024-6028	IN	denotes	with
T14399	6029-6035	JJ	denotes	lambda
T14400	6036-6047	NN	denotes	phosphatase
T14401	6048-6049	-LRB-	denotes	(
T14402	6049-6052	NNP	denotes	New
T14403	6053-6060	NNP	denotes	England
T14404	6061-6068	NNP	denotes	BioLabs
T14405	6068-6069	-RRB-	denotes	)
T14406	6070-6072	IN	denotes	as
T14407	6073-6076	IN	denotes	per
T14408	6077-6089	NN	denotes	manufacturer
T14409	6089-6091	POS	denotes	's
T14410	6092-6104	NNS	denotes	instructions
T14411	6104-6105	.	denotes	.
T14412	6106-6111	NNS	denotes	Beads
T14413	6112-6116	VBD	denotes	were
T14414	6117-6128	VBN	denotes	resuspended
T14415	6129-6131	IN	denotes	in
T14416	6132-6134	NNP	denotes	2X
T14417	6135-6143	NNP	denotes	SDS-PAGE
T14418	6144-6150	NN	denotes	sample
T14419	6151-6157	NN	denotes	buffer
T14420	6158-6161	CC	denotes	and
T14421	6162-6168	VBN	denotes	heated
T14422	6169-6172	IN	denotes	for
T14423	6173-6174	CD	denotes	5
T14424	6175-6182	NNS	denotes	minutes
T14425	6183-6185	IN	denotes	at
T14426	6186-6188	CD	denotes	95
T14427	6188-6189	CD	denotes	°
T14428	6189-6191	NNP	denotes	C.
T14429	6192-6200	NNP	denotes	Material
T14430	6201-6207	VBD	denotes	eluted
T14431	6208-6212	IN	denotes	from
T14432	6213-6216	DT	denotes	the
T14433	6217-6222	NNS	denotes	beads
T14434	6223-6226	VBD	denotes	was
T14435	6227-6239	VBN	denotes	fractionated
T14436	6240-6242	IN	denotes	by
T14437	6243-6251	NNP	denotes	SDS-PAGE
T14438	6252-6255	CC	denotes	and
T14439	6256-6267	VBN	denotes	transferred
T14440	6268-6270	TO	denotes	to
T14441	6271-6275	NNP	denotes	PVDF
T14442	6276-6285	NNS	denotes	membranes
T14443	6286-6289	IN	denotes	for
T14444	6290-6297	JJ	denotes	Western
T14445	6298-6306	VBG	denotes	blotting
T14446	6306-6307	.	denotes	.
T14447	6308-6310	IN	denotes	In
T14448	6311-6315	DT	denotes	some
T14449	6316-6327	NNS	denotes	experiments
T14450	6327-6328	,	denotes	,
T14451	6329-6334	NNS	denotes	cells
T14452	6335-6339	VBD	denotes	were
T14453	6340-6348	VBN	denotes	cultured
T14454	6349-6352	IN	denotes	for
T14455	6353-6354	CD	denotes	6
T14456	6355-6358	NNS	denotes	hrs
T14457	6359-6363	IN	denotes	with
T14458	6364-6366	CD	denotes	25
T14459	6367-6369	NNP	denotes	µM
T14460	6370-6389	JJ	denotes	antennapedia-linked
T14461	6390-6397	NNP	denotes	SMAC-N7
T14462	6398-6405	NN	denotes	peptide
T14463	6406-6407	-LRB-	denotes	(
T14464	6407-6417	NNP	denotes	Calbiochem
T14465	6417-6418	-RRB-	denotes	)
T14466	6419-6421	CC	denotes	or
T14467	6422-6424	DT	denotes	an
T14468	6425-6436	JJ	denotes	appropriate
T14469	6437-6443	NN	denotes	volume
T14470	6444-6446	IN	denotes	of
T14471	6447-6450	DT	denotes	the
T14472	6451-6458	JJ	denotes	solvent
T14473	6459-6463	VBN	denotes	used
T14474	6464-6467	IN	denotes	for
T14475	6468-6471	DT	denotes	the
T14476	6472-6479	NN	denotes	peptide
T14477	6480-6481	-LRB-	denotes	(
T14478	6481-6485	NNP	denotes	DMSO
T14479	6485-6486	-RRB-	denotes	)
T14480	6486-6487	.	denotes	.
T14481	6488-6493	IN	denotes	After
T14482	6494-6504	NN	denotes	incubation
T14483	6504-6505	,	denotes	,
T14484	6506-6511	NNS	denotes	cells
T14485	6512-6513	-LRB-	denotes	(
T14486	6513-6515	IN	denotes	in
T14487	6516-6523	NN	denotes	peptide
T14488	6523-6524	:	denotes	-
T14489	6525-6527	CC	denotes	or
T14490	6528-6543	JJ	denotes	DMSO-containing
T14491	6544-6550	NN	denotes	medium
T14492	6550-6551	-RRB-	denotes	)
T14493	6552-6556	VBD	denotes	were
T14494	6557-6567	VBN	denotes	stimulated
T14495	6568-6572	IN	denotes	with
T14496	6573-6588	JJ	denotes	antibody-coated
T14497	6589-6594	NNS	denotes	beads
T14498	6595-6597	IN	denotes	as
T14499	6598-6606	VBN	denotes	outlined
T14500	6607-6612	IN	denotes	above
T14501	6612-6613	.	denotes	.
R9246	T11924	T11925	compound	Cell,lines
R9247	T11925	T11937	nsubjpass	lines,described
R9248	T11926	T11930	det	The,line
R9249	T11927	T11929	compound	mouse,cell
R9250	T11928	T11929	compound	B,cell
R9251	T11929	T11930	compound	cell,line
R9252	T11930	T11937	nsubjpass	line,described
R9253	T11931	T11933	compound	A20,J
R9254	T11932	T11933	compound	.2,J
R9255	T11933	T11930	appos	J,line
R9256	T11934	T11937	aux	has,described
R9257	T11935	T11937	auxpass	been,described
R9258	T11936	T11937	advmod	previously,described
R9259	T11937	T11937	ROOT	described,described
R9260	T11938	T11940	nmod	[,]
R9261	T11939	T11940	nummod	26,]
R9262	T11940	T11937	dobj	],described
R9263	T11941	T11940	punct	",",]
R9264	T11942	T11944	nmod	[,]
R9265	T11943	T11944	nummod	27,]
R9266	T11944	T11940	appos	],]
R9267	T11945	T11937	punct	.,described
R9268	T11946	T11948	nsubjpass	Cells,grown
R9269	T11947	T11948	auxpass	were,grown
R9270	T11948	T11948	ROOT	grown,grown
R9271	T11949	T11948	prep	in,grown
R9272	T11950	T11949	pobj	RPMI,in
R9273	T11951	T11950	nummod	1640,RPMI
R9274	T11952	T11950	punct	",",RPMI
R9275	T11953	T11954	nummod	10,%
R9276	T11954	T11955	npadvmod	%,FCS
R9277	T11955	T11950	appos	FCS,RPMI
R9278	T11956	T11955	punct	",",FCS
R9279	T11957	T11959	nummod	10,2-ME
R9280	T11958	T11959	compound	µM,2-ME
R9281	T11959	T11955	appos	2-ME,FCS
R9282	T11960	T11955	punct	",",FCS
R9283	T11961	T11963	nummod	2,L-glutamine
R9284	T11962	T11963	compound	mM,L-glutamine
R9285	T11963	T11955	appos	L-glutamine,FCS
R9286	T11964	T11963	punct	",",L-glutamine
R9287	T11965	T11963	cc	and,L-glutamine
R9288	T11966	T11955	appos	antibiotics,FCS
R9289	T11967	T11948	punct	.,grown
R9290	T11968	T11970	nummod	HI-5,cells
R9291	T11969	T11970	compound	insect,cells
R9292	T11970	T11978	nsubjpass	cells,described
R9293	T11971	T11972	punct	(,Invitrogen
R9294	T11972	T11970	appos	Invitrogen,cells
R9295	T11973	T11970	punct	),cells
R9296	T11974	T11970	acl	expressing,cells
R9297	T11975	T11974	dobj	CD154,expressing
R9298	T11976	T11978	aux	have,described
R9299	T11977	T11978	auxpass	been,described
R9300	T11978	T11978	ROOT	described,described
R9301	T11979	T11978	dobj	[,described
R9302	T11980	T11981	nummod	28,]
R9303	T11981	T11979	appos	],[
R9304	T11982	T11978	punct	.,described
R9364	T12059	T12103	nsubj	Antibodies,were
R9365	T12060	T12063	nmod	Mouse,antibody
R9366	T12061	T12063	amod	anti-birch,antibody
R9367	T12062	T12063	compound	profilin,antibody
R9368	T12063	T12103	nsubj	antibody,were
R9369	T12064	T12066	punct	(,[
R9370	T12065	T12066	compound	4A6,[
R9371	T12066	T12063	appos	[,antibody
R9372	T12067	T12066	nummod	29,[
R9373	T12068	T12066	appos	],[
R9374	T12069	T12066	punct	),[
R9375	T12070	T12066	punct	",",[
R9376	T12071	T12073	compound	rat,CD40
R9377	T12072	T12073	compound	anti-mouse,CD40
R9378	T12073	T12066	conj	CD40,[
R9379	T12074	T12076	punct	(,[
R9380	T12075	T12076	compound	1C10,[
R9381	T12076	T12078	npadvmod	[,]
R9382	T12077	T12076	nummod	30,[
R9383	T12078	T12073	appos	],CD40
R9384	T12079	T12078	punct	),]
R9385	T12080	T12073	cc	and,CD40
R9386	T12081	T12085	det	a,antibody
R9387	T12082	T12085	nmod	rat,antibody
R9388	T12083	T12085	compound	isotype,antibody
R9389	T12084	T12085	compound	control,antibody
R9390	T12085	T12073	conj	antibody,CD40
R9391	T12086	T12087	punct	(,mAb72
R9392	T12087	T12085	appos	mAb72,antibody
R9393	T12088	T12087	punct	",",mAb72
R9394	T12089	T12092	compound	Developmental,Bank
R9395	T12090	T12092	compound	Studies,Bank
R9396	T12091	T12092	compound	Hybridoma,Bank
R9397	T12092	T12085	conj	Bank,antibody
R9398	T12093	T12092	punct	",",Bank
R9399	T12094	T12092	conj	University,Bank
R9400	T12095	T12094	prep	of,University
R9401	T12096	T12095	pobj	Iowa,of
R9402	T12097	T12096	punct	",",Iowa
R9403	T12098	T12099	compound	Iowa,City
R9404	T12099	T12094	conj	City,University
R9405	T12100	T12099	punct	",",City
R9406	T12101	T12099	conj	IA,City
R9407	T12102	T12092	punct	),Bank
R9408	T12103	T12103	ROOT	were,were
R9409	T12104	T12103	acomp	isolated,were
R9410	T12105	T12104	prep	from,isolated
R9411	T12106	T12107	compound	hybridoma,supernatants
R9412	T12107	T12105	pobj	supernatants,from
R9413	T12108	T12103	punct	.,were
R9414	T12109	T12111	amod	FITC-labeled,CD80
R9415	T12110	T12111	amod	anti-mouse,CD80
R9416	T12111	T12117	nsubj	CD80,were
R9417	T12112	T12111	cc	and,CD80
R9418	T12113	T12115	det	an,control
R9419	T12114	T12115	compound	isotype,control
R9420	T12115	T12111	conj	control,CD80
R9421	T12116	T12117	nsubj	Ab,were
R9422	T12117	T12117	ROOT	were,were
R9423	T12118	T12117	prep	from,were
R9424	T12119	T12118	pobj	eBiosciences,from
R9425	T12120	T12117	punct	.,were
R9426	T12121	T12126	nmod	Rabbit,]
R9427	T12122	T12126	nmod	anti-HOIP,]
R9428	T12123	T12124	compound	Ab,[
R9429	T12124	T12122	appos	[,anti-HOIP
R9430	T12125	T12126	nummod	6,]
R9431	T12126	T12127	nsubj	],was
R9432	T12127	T12127	ROOT	was,was
R9433	T12128	T12130	det	the,gift
R9434	T12129	T12130	compound	kind,gift
R9435	T12130	T12127	attr	gift,was
R9436	T12131	T12130	prep	of,gift
R9437	T12132	T12134	compound	Dr.,Eipper
R9438	T12133	T12134	compound	Betty,Eipper
R9439	T12134	T12131	pobj	Eipper,of
R9440	T12135	T12134	punct	(,Eipper
R9441	T12136	T12134	appos	University,Eipper
R9442	T12137	T12136	prep	of,University
R9443	T12138	T12137	pobj	Connecticut,of
R9444	T12139	T12140	compound	Health,Center
R9445	T12140	T12134	appos	Center,Eipper
R9446	T12141	T12140	punct	",",Center
R9447	T12142	T12134	npadvmod	Farmington,Eipper
R9448	T12143	T12142	punct	",",Farmington
R9449	T12144	T12142	appos	Connecticut,Farmington
R9450	T12145	T12142	punct	),Farmington
R9451	T12146	T12127	punct	.,was
R9452	T12147	T12149	compound	Goat,IgG
R9453	T12148	T12149	compound	anti-rat,IgG
R9454	T12149	T12155	nsubj	IgG,were
R9455	T12150	T12149	punct	",",IgG
R9456	T12151	T12149	cc	and,IgG
R9457	T12152	T12154	amod	HRP-labeled,Abs
R9458	T12153	T12154	amod	secondary,Abs
R9459	T12154	T12149	conj	Abs,IgG
R9460	T12155	T12155	ROOT	were,were
R9461	T12156	T12155	prep	from,were
R9462	T12157	T12159	compound	Jackson,Laboratories
R9463	T12158	T12159	compound	ImmunoResearch,Laboratories
R9464	T12159	T12156	pobj	Laboratories,from
R9465	T12160	T12159	punct	",",Laboratories
R9466	T12161	T12159	conj	Inc.,Laboratories
R9467	T12162	T12155	punct	.,were
R9585	T12332	T12334	amod	Somatic,gene
R9586	T12333	T12334	compound	cell,gene
R9587	T12334	T12342	nsubjpass	gene,accomplished
R9588	T12335	T12334	acl	targeting,gene
R9589	T12336	T12337	det	The,generation
R9590	T12337	T12335	dobj	generation,targeting
R9591	T12338	T12337	prep	of,generation
R9592	T12339	T12340	amod	HOIP-deficient,cells
R9593	T12340	T12338	pobj	cells,of
R9594	T12341	T12342	auxpass	was,accomplished
R9595	T12342	T12342	ROOT	accomplished,accomplished
R9596	T12343	T12342	advcl	using,accomplished
R9597	T12344	T12347	det	a,approach
R9598	T12345	T12347	amod	homologous,approach
R9599	T12346	T12347	compound	recombination,approach
R9600	T12347	T12343	dobj	approach,using
R9601	T12348	T12342	conj	described,accomplished
R9602	T12349	T12350	advmod	previously,[
R9603	T12350	T12352	amod	[,]
R9604	T12351	T12352	nummod	7,]
R9605	T12352	T12348	dobj	],described
R9606	T12353	T12352	punct	",",]
R9607	T12354	T12356	nmod	[,]
R9608	T12355	T12356	nummod	8,]
R9609	T12356	T12352	appos	],]
R9610	T12357	T12342	punct	.,accomplished
R9611	T12358	T12367	nsubj	Segments,construct
R9612	T12359	T12358	prep	of,Segments
R9613	T12360	T12362	amod	Rnf31,sequence
R9614	T12361	T12362	compound	gene,sequence
R9615	T12362	T12359	pobj	sequence,of
R9616	T12363	T12362	acl	used,sequence
R9617	T12364	T12363	prep	in,used
R9618	T12365	T12366	det	the,targeting
R9619	T12366	T12364	pobj	targeting,in
R9620	T12367	T12367	ROOT	construct,construct
R9621	T12368	T12370	punct	(,1A
R9622	T12369	T12370	compound	Fig.,1A
R9623	T12370	T12367	dobj	1A,construct
R9624	T12371	T12367	punct	),construct
R9625	T12372	T12373	auxpass	were,amplified
R9626	T12373	T12367	advcl	amplified,construct
R9627	T12374	T12373	agent	by,amplified
R9628	T12375	T12374	pobj	PCR,by
R9629	T12376	T12373	prep	from,amplified
R9630	T12377	T12379	nmod	A20,J
R9631	T12378	T12379	nummod	.2,J
R9632	T12379	T12381	nmod	J,DNA
R9633	T12380	T12381	amod	genomic,DNA
R9634	T12381	T12376	pobj	DNA,from
R9635	T12382	T12367	punct	.,construct
R9636	T12383	T12385	det	The,primers
R9637	T12384	T12385	compound	oligonucleotide,primers
R9638	T12385	T12397	nsubj	primers,were
R9639	T12386	T12385	acl	used,primers
R9640	T12387	T12388	aux	to,generate
R9641	T12388	T12386	xcomp	generate,used
R9642	T12389	T12392	det	the,flank
R9643	T12390	T12392	nummod	5,flank
R9644	T12391	T12392	compound	′,flank
R9645	T12392	T12388	dobj	flank,generate
R9646	T12393	T12392	punct	(,flank
R9647	T12394	T12395	nummod	1224,bp
R9648	T12395	T12392	appos	bp,flank
R9649	T12396	T12392	punct	),flank
R9650	T12397	T12397	ROOT	were,were
R9651	T12398	T12401	nummod	5,ttttctagagcggtggcttaagtgaccc-3
R9652	T12399	T12401	punct	′,ttttctagagcggtggcttaagtgaccc-3
R9653	T12400	T12401	punct	-,ttttctagagcggtggcttaagtgaccc-3
R9654	T12401	T12402	compound	ttttctagagcggtggcttaagtgaccc-3,′
R9655	T12402	T12397	attr	′,were
R9656	T12403	T12402	cc	and,′
R9657	T12404	T12407	nummod	5,tattctagatgcagcatctgagaaagcaagc-3
R9658	T12405	T12407	punct	′,tattctagatgcagcatctgagaaagcaagc-3
R9659	T12406	T12407	punct	-,tattctagatgcagcatctgagaaagcaagc-3
R9660	T12407	T12408	amod	tattctagatgcagcatctgagaaagcaagc-3,′
R9661	T12408	T12402	conj	′,′
R9662	T12409	T12397	punct	.,were
R9663	T12410	T12413	det	The,flank
R9664	T12411	T12412	nummod	3,′
R9665	T12412	T12413	compound	′,flank
R9666	T12413	T12419	nsubj	flank,were
R9667	T12414	T12413	punct	(,flank
R9668	T12415	T12413	appos	6032,flank
R9669	T12416	T12413	appos	bp,flank
R9670	T12417	T12413	punct	),flank
R9671	T12418	T12419	nsubj	primers,were
R9672	T12419	T12419	ROOT	were,were
R9673	T12420	T12424	nummod	5,′
R9674	T12421	T12423	punct	′,aaaaccggtgtatgcttctttacgggagaaaaatattag-3
R9675	T12422	T12423	punct	-,aaaaccggtgtatgcttctttacgggagaaaaatattag-3
R9676	T12423	T12424	compound	aaaaccggtgtatgcttctttacgggagaaaaatattag-3,′
R9677	T12424	T12419	attr	′,were
R9678	T12425	T12424	cc	and,′
R9679	T12426	T12429	nummod	5,tataccggtatgaagccaaaggaacactgagag-3
R9680	T12427	T12429	punct	′,tataccggtatgaagccaaaggaacactgagag-3
R9681	T12428	T12429	punct	-,tataccggtatgaagccaaaggaacactgagag-3
R9682	T12429	T12430	compound	tataccggtatgaagccaaaggaacactgagag-3,′
R9683	T12430	T12424	conj	′,′
R9684	T12431	T12419	punct	.,were
R9685	T12432	T12434	compound	Restriction,sites
R9686	T12433	T12434	compound	endonuclease,sites
R9687	T12434	T12434	ROOT	sites,sites
R9688	T12435	T12434	prep	in,sites
R9689	T12436	T12438	det	the,primers
R9690	T12437	T12438	compound	oligonucleotide,primers
R9691	T12438	T12435	pobj	primers,in
R9692	T12439	T12440	amod	allowed,insertion
R9693	T12440	T12434	conj	insertion,sites
R9694	T12441	T12440	prep	of,insertion
R9695	T12442	T12444	det	the,products
R9696	T12443	T12444	compound	PCR,products
R9697	T12444	T12441	pobj	products,of
R9698	T12445	T12434	prep	into,sites
R9699	T12446	T12448	det	the,vector
R9700	T12447	T12448	compound	targeting,vector
R9701	T12448	T12445	pobj	vector,into
R9702	T12449	T12434	punct	.,sites
R9703	T12450	T12453	nummod	A20,cells
R9704	T12451	T12453	nummod	.2,cells
R9705	T12452	T12453	compound	J,cells
R9706	T12453	T12455	nsubjpass	cells,transfected
R9707	T12454	T12455	auxpass	were,transfected
R9708	T12455	T12455	ROOT	transfected,transfected
R9709	T12456	T12455	punct	(,transfected
R9710	T12457	T12455	agent	by,transfected
R9711	T12458	T12459	compound	electroporation,[
R9712	T12459	T12457	pobj	[,by
R9713	T12460	T12461	nummod	7,]
R9714	T12461	T12459	appos	],[
R9715	T12462	T12461	punct	),]
R9716	T12463	T12455	prep	with,transfected
R9717	T12464	T12465	det	the,targeting
R9718	T12465	T12463	pobj	targeting,with
R9719	T12466	T12455	conj	construct,transfected
R9720	T12467	T12466	cc	and,construct
R9721	T12468	T12466	conj	subcloned,construct
R9722	T12469	T12468	prep	in,subcloned
R9723	T12470	T12469	pobj	medium,in
R9724	T12471	T12470	acl	containing,medium
R9725	T12472	T12476	nummod	600,G418
R9726	T12473	T12476	compound	µg,G418
R9727	T12474	T12476	compound	/,G418
R9728	T12475	T12476	compound	ml,G418
R9729	T12476	T12477	compound	G418,sulfate
R9730	T12477	T12471	dobj	sulfate,containing
R9731	T12478	T12455	punct	.,transfected
R9732	T12479	T12480	amod	Homologous,recombination
R9733	T12480	T12485	nsubjpass	recombination,detected
R9734	T12481	T12480	prep	in,recombination
R9735	T12482	T12483	amod	G418-resistant,clones
R9736	T12483	T12481	pobj	clones,in
R9737	T12484	T12485	auxpass	was,detected
R9738	T12485	T12485	ROOT	detected,detected
R9739	T12486	T12485	agent	by,detected
R9740	T12487	T12486	pobj	PCR,by
R9741	T12488	T12487	prep	of,PCR
R9742	T12489	T12490	amod	genomic,DNA
R9743	T12490	T12488	pobj	DNA,of
R9744	T12491	T12485	punct	",",detected
R9745	T12492	T12493	mark	as,described
R9746	T12493	T12485	advcl	described,detected
R9747	T12494	T12496	nmod	[,]
R9748	T12495	T12496	nummod	7,]
R9749	T12496	T12493	dobj	],described
R9750	T12497	T12485	punct	.,detected
R9751	T12498	T12499	compound	Oligonucleotide,primers
R9752	T12499	T12503	nsubj	primers,were
R9753	T12500	T12499	acl	used,primers
R9754	T12501	T12500	prep	for,used
R9755	T12502	T12501	pobj	screening,for
R9756	T12503	T12517	ccomp	were,noted
R9757	T12504	T12507	nummod	5,cttcctgatctcagctttaccgtcac-3
R9758	T12505	T12507	punct	′,cttcctgatctcagctttaccgtcac-3
R9759	T12506	T12507	punct	-,cttcctgatctcagctttaccgtcac-3
R9760	T12507	T12508	amod	cttcctgatctcagctttaccgtcac-3,′
R9761	T12508	T12503	attr	′,were
R9762	T12509	T12510	punct	(,homologous
R9763	T12510	T12517	ccomp	homologous,noted
R9764	T12511	T12510	prep	to,homologous
R9765	T12512	T12513	amod	genomic,sequence
R9766	T12513	T12511	pobj	sequence,to
R9767	T12514	T12517	punct	;,noted
R9768	T12515	T12516	amod	approximate,position
R9769	T12516	T12517	nsubj	position,noted
R9770	T12517	T12517	ROOT	noted,noted
R9771	T12518	T12517	prep	in,noted
R9772	T12519	T12518	pobj	Fig.,in
R9773	T12520	T12519	nummod	1,Fig.
R9774	T12521	T12517	punct	),noted
R9775	T12522	T12517	cc	and,noted
R9776	T12523	T12526	nummod	5,caatccatcttgttcagccat-3
R9777	T12524	T12526	punct	′,caatccatcttgttcagccat-3
R9778	T12525	T12526	punct	-,caatccatcttgttcagccat-3
R9779	T12526	T12527	compound	caatccatcttgttcagccat-3,′
R9780	T12527	T12527	ROOT	′,′
R9781	T12528	T12529	punct	(,homologous
R9782	T12529	T12527	parataxis	homologous,′
R9783	T12530	T12531	aux	to,sequence
R9784	T12531	T12529	relcl	sequence,homologous
R9785	T12532	T12531	prep	in,sequence
R9786	T12533	T12532	pobj	NeoR,in
R9787	T12534	T12529	punct	),homologous
R9788	T12535	T12517	punct	.,noted
R9789	T12536	T12548	nsubjpass	Clones,transfected
R9790	T12537	T12545	prep	in,disrupted
R9791	T12538	T12537	pobj	which,in
R9792	T12539	T12540	nummod	one,copy
R9793	T12540	T12545	nsubjpass	copy,disrupted
R9794	T12541	T12540	prep	of,copy
R9795	T12542	T12541	pobj	Rnf31,of
R9796	T12543	T12545	aux	had,disrupted
R9797	T12544	T12545	auxpass	been,disrupted
R9798	T12545	T12536	relcl	disrupted,Clones
R9799	T12546	T12548	auxpass	were,transfected
R9800	T12547	T12548	advmod	transiently,transfected
R9801	T12548	T12548	ROOT	transfected,transfected
R9802	T12549	T12548	prep	with,transfected
R9803	T12550	T12552	det	an,plasmid
R9804	T12551	T12552	compound	expression,plasmid
R9805	T12552	T12549	pobj	plasmid,with
R9806	T12553	T12554	compound	encoding,Cre
R9807	T12554	T12548	npadvmod	Cre,transfected
R9808	T12555	T12548	punct	",",transfected
R9809	T12556	T12548	prep	in,transfected
R9810	T12557	T12556	pobj	order,in
R9811	T12558	T12559	aux	to,remove
R9812	T12559	T12557	acl	remove,order
R9813	T12560	T12559	dobj	NeoR,remove
R9814	T12561	T12548	punct	.,transfected
R9815	T12562	T12563	amod	G418-sensitive,subclones
R9816	T12563	T12565	nsubjpass	subclones,subjected
R9817	T12564	T12565	auxpass	were,subjected
R9818	T12565	T12565	ROOT	subjected,subjected
R9819	T12566	T12565	prep	to,subjected
R9820	T12567	T12569	det	a,round
R9821	T12568	T12569	amod	second,round
R9822	T12569	T12566	pobj	round,to
R9823	T12570	T12569	prep	of,round
R9824	T12571	T12570	pobj	targeting,of
R9825	T12572	T12573	aux	to,disrupt
R9826	T12573	T12571	xcomp	disrupt,targeting
R9827	T12574	T12576	det	the,copy
R9828	T12575	T12576	amod	remaining,copy
R9829	T12576	T12573	dobj	copy,disrupt
R9830	T12577	T12576	prep	of,copy
R9831	T12578	T12577	pobj	Rnf31,of
R9832	T12579	T12565	punct	.,subjected
R9833	T12580	T12581	amod	G418-resistant,clones
R9834	T12581	T12583	nsubjpass	clones,tested
R9835	T12582	T12583	auxpass	were,tested
R9836	T12583	T12583	ROOT	tested,tested
R9837	T12584	T12583	prep	for,tested
R9838	T12585	T12586	amod	homologous,recombination
R9839	T12586	T12584	pobj	recombination,for
R9840	T12587	T12586	prep	by,recombination
R9841	T12588	T12587	pobj	PCR,by
R9842	T12589	T12588	cc	and,PCR
R9843	T12590	T12592	compound	HOIP,expression
R9844	T12591	T12592	compound	protein,expression
R9845	T12592	T12583	dobj	expression,tested
R9846	T12593	T12583	agent	by,tested
R9847	T12594	T12595	amod	Western,blot
R9848	T12595	T12593	pobj	blot,by
R9849	T12596	T12583	punct	.,tested
R10097	T12911	T12912	compound	Retroviral,transduction
R10098	T12912	T12912	ROOT	transduction,transduction
R10099	T12913	T12915	det	A,vector
R10100	T12914	T12915	amod	retroviral,vector
R10101	T12915	T12920	nsubjpass	vector,used
R10102	T12916	T12917	punct	(,pMIP
R10103	T12917	T12915	appos	pMIP,vector
R10104	T12918	T12917	punct	),pMIP
R10105	T12919	T12920	auxpass	was,used
R10106	T12920	T12920	ROOT	used,used
R10107	T12921	T12920	prep	for,used
R10108	T12922	T12924	det	the,transduction
R10109	T12923	T12924	amod	stable,transduction
R10110	T12924	T12921	pobj	transduction,for
R10111	T12925	T12924	prep	of,transduction
R10112	T12926	T12925	pobj	cells,of
R10113	T12927	T12924	prep	with,transduction
R10114	T12928	T12932	nmod	HOIP,constructs
R10115	T12929	T12928	cc	and,HOIP
R10116	T12930	T12931	compound	IKKγ,cDNA
R10117	T12931	T12928	conj	cDNA,HOIP
R10118	T12932	T12927	pobj	constructs,with
R10119	T12933	T12920	punct	.,used
R10120	T12934	T12936	nsubjpass	pMIP,constructed
R10121	T12935	T12936	auxpass	was,constructed
R10122	T12936	T12936	ROOT	constructed,constructed
R10123	T12937	T12936	prep	by,constructed
R10124	T12938	T12937	pcomp	replacing,by
R10125	T12939	T12940	det	the,IRES
R10126	T12940	T12938	dobj	IRES,replacing
R10127	T12941	T12940	cc	and,IRES
R10128	T12942	T12940	conj	GFP,IRES
R10129	T12943	T12944	amod	encoding,sequences
R10130	T12944	T12938	dobj	sequences,replacing
R10131	T12945	T12938	prep	in,replacing
R10132	T12946	T12947	compound	pMIG,[
R10133	T12947	T12945	pobj	[,in
R10134	T12948	T12949	nummod	31,]
R10135	T12949	T12947	appos	],[
R10136	T12950	T12949	prep	with,]
R10137	T12951	T12952	det	the,IRES
R10138	T12952	T12956	nmod	IRES,gene
R10139	T12953	T12952	cc	and,IRES
R10140	T12954	T12956	amod	puromycin,gene
R10141	T12955	T12956	compound	resistance,gene
R10142	T12956	T12950	pobj	gene,with
R10143	T12957	T12956	prep	from,gene
R10144	T12958	T12957	pobj	pIRESpuro2,from
R10145	T12959	T12958	punct	(,pIRESpuro2
R10146	T12960	T12958	appos	Clontech,pIRESpuro2
R10147	T12961	T12958	punct	),pIRESpuro2
R10148	T12962	T12936	punct	.,constructed
R10149	T12963	T12964	amod	FLAG-tagged,mouse
R10150	T12964	T12966	compound	mouse,cDNA
R10151	T12965	T12966	compound	HOIP,cDNA
R10152	T12966	T12968	nsubjpass	cDNA,inserted
R10153	T12967	T12968	auxpass	was,inserted
R10154	T12968	T12968	ROOT	inserted,inserted
R10155	T12969	T12968	prep	into,inserted
R10156	T12970	T12973	det	the,site
R10157	T12971	T12973	amod	multiple,site
R10158	T12972	T12973	compound	cloning,site
R10159	T12973	T12969	pobj	site,into
R10160	T12974	T12973	prep	of,site
R10161	T12975	T12974	pobj	pMIP,of
R10162	T12976	T12968	punct	.,inserted
R10163	T12977	T12978	det	A,pMIP
R10164	T12978	T12979	nsubj	pMIP,construct
R10165	T12979	T12979	ROOT	construct,construct
R10166	T12980	T12981	amod	encoding,mouse
R10167	T12981	T12982	compound	mouse,IKKγ
R10168	T12982	T12979	dobj	IKKγ,construct
R10169	T12983	T12979	prep	with,construct
R10170	T12984	T12989	det	an,peptide
R10171	T12985	T12987	amod	amino,birch
R10172	T12986	T12987	compound	terminal,birch
R10173	T12987	T12989	compound	birch,peptide
R10174	T12988	T12989	compound	profilin,peptide
R10175	T12989	T12983	pobj	peptide,with
R10176	T12990	T12991	punct	(,BP
R10177	T12991	T12989	appos	BP,peptide
R10178	T12992	T12979	punct	),construct
R10179	T12993	T12994	punct	-,tag
R10180	T12994	T12997	nsubjpass	tag,prepared
R10181	T12995	T12997	auxpass	was,prepared
R10182	T12996	T12997	advmod	also,prepared
R10183	T12997	T12979	conj	prepared,construct
R10184	T12998	T12997	punct	.,prepared
R10185	T12999	T13000	compound	Retroviral,particles
R10186	T13000	T13002	nsubjpass	particles,generated
R10187	T13001	T13002	auxpass	were,generated
R10188	T13002	T13002	ROOT	generated,generated
R10189	T13003	T13002	agent	by,generated
R10190	T13004	T13005	amod	transient,transfection
R10191	T13005	T13003	pobj	transfection,by
R10192	T13006	T13005	prep	of,transfection
R10193	T13007	T13008	amod	293T,cells
R10194	T13008	T13006	pobj	cells,of
R10195	T13009	T13005	prep	with,transfection
R10196	T13010	T13009	pobj	pMIP,with
R10197	T13011	T13010	punct	",",pMIP
R10198	T13012	T13010	conj	pMIP-HOIP,pMIP
R10199	T13013	T13012	punct	",",pMIP-HOIP
R10200	T13014	T13012	cc	or,pMIP-HOIP
R10201	T13015	T13012	conj	pMIP-IKKγ,pMIP-HOIP
R10202	T13016	T13015	cc	and,pMIP-IKKγ
R10203	T13017	T13019	det	the,vector
R10204	T13018	T13019	compound	packaging,vector
R10205	T13019	T13023	nmod	vector,]
R10206	T13020	T13021	compound	pCL-Eco,[
R10207	T13021	T13019	appos	[,vector
R10208	T13022	T13023	nummod	32,]
R10209	T13023	T13015	conj	],pMIP-IKKγ
R10210	T13024	T13002	punct	.,generated
R10211	T13025	T13026	nummod	Two,days
R10212	T13026	T13027	npadvmod	days,after
R10213	T13027	T13033	prep	after,harvested
R10214	T13028	T13027	pobj	transfection,after
R10215	T13029	T13033	punct	",",harvested
R10216	T13030	T13031	compound	culture,supernatants
R10217	T13031	T13033	nsubjpass	supernatants,harvested
R10218	T13032	T13033	auxpass	were,harvested
R10219	T13033	T13033	ROOT	harvested,harvested
R10220	T13034	T13033	cc	and,harvested
R10221	T13035	T13033	conj	filtered,harvested
R10222	T13036	T13033	punct	.,harvested
R10223	T13037	T13039	nummod	A20,J
R10224	T13038	T13039	nummod	.2,J
R10225	T13039	T13042	nmod	J,cells
R10226	T13040	T13039	cc	or,J
R10227	T13041	T13039	conj	HOIP-deficient,J
R10228	T13042	T13044	nsubjpass	cells,added
R10229	T13043	T13044	auxpass	were,added
R10230	T13044	T13044	ROOT	added,added
R10231	T13045	T13044	prep	to,added
R10232	T13046	T13047	amod	24-well,plates
R10233	T13047	T13045	pobj	plates,to
R10234	T13048	T13047	punct	(,plates
R10235	T13049	T13052	nummod	2.5,cells/well
R10236	T13050	T13052	nummod	×,cells/well
R10237	T13051	T13052	nummod	105,cells/well
R10238	T13052	T13047	appos	cells/well,plates
R10239	T13053	T13047	punct	),plates
R10240	T13054	T13044	prep	with,added
R10241	T13055	T13056	nummod	1,ml
R10242	T13056	T13058	nmod	ml,supernatant
R10243	T13057	T13058	amod	virus-containing,supernatant
R10244	T13058	T13054	pobj	supernatant,with
R10245	T13059	T13058	cc	and,supernatant
R10246	T13060	T13061	nummod	8,µg
R10247	T13061	T13064	nmod	µg,polybrene
R10248	T13062	T13064	nmod	/,polybrene
R10249	T13063	T13064	compound	ml,polybrene
R10250	T13064	T13058	conj	polybrene,supernatant
R10251	T13065	T13044	punct	.,added
R10252	T13066	T13068	nsubjpass	Plates,centrifuged
R10253	T13067	T13068	auxpass	were,centrifuged
R10254	T13068	T13068	ROOT	centrifuged,centrifuged
R10255	T13069	T13068	prep	at,centrifuged
R10256	T13070	T13072	nummod	500,g
R10257	T13071	T13072	punct	x,g
R10258	T13072	T13069	pobj	g,at
R10259	T13073	T13072	prep	for,g
R10260	T13074	T13075	nummod	2,hrs
R10261	T13075	T13073	pobj	hrs,for
R10262	T13076	T13072	prep	at,g
R10263	T13077	T13078	compound	room,temperature
R10264	T13078	T13076	pobj	temperature,at
R10265	T13079	T13068	punct	.,centrifuged
R10266	T13080	T13082	nsubjpass	Cells,cultured
R10267	T13081	T13082	auxpass	were,cultured
R10268	T13082	T13082	ROOT	cultured,cultured
R10269	T13083	T13082	prep	in,cultured
R10270	T13084	T13086	compound	B,culture
R10271	T13085	T13086	compound	cell,culture
R10272	T13086	T13087	compound	culture,medium
R10273	T13087	T13083	pobj	medium,in
R10274	T13088	T13082	prep	for,cultured
R10275	T13089	T13090	nummod	48,hrs
R10276	T13090	T13088	pobj	hrs,for
R10277	T13091	T13082	punct	",",cultured
R10278	T13092	T13098	prep	after,selected
R10279	T13093	T13092	pobj	which,after
R10280	T13094	T13096	det	the,cells
R10281	T13095	T13096	amod	transduced,cells
R10282	T13096	T13098	nsubjpass	cells,selected
R10283	T13097	T13098	auxpass	were,selected
R10284	T13098	T13082	advcl	selected,cultured
R10285	T13099	T13098	prep	in,selected
R10286	T13100	T13101	nummod	3,µg
R10287	T13101	T13104	nmod	µg,puromycin
R10288	T13102	T13104	nmod	/,puromycin
R10289	T13103	T13104	compound	ml,puromycin
R10290	T13104	T13099	pobj	puromycin,in
R10291	T13105	T13082	punct	.,cultured
R10480	T13347	T13348	nummod	CD80,upregulation
R10481	T13348	T13361	npadvmod	upregulation,were
R10482	T13349	T13348	cc	and,upregulation
R10483	T13350	T13352	compound	flow,Cells
R10484	T13351	T13352	compound	cytometry,Cells
R10485	T13352	T13348	conj	Cells,upregulation
R10486	T13353	T13354	punct	(,5
R10487	T13354	T13352	nummod	5,Cells
R10488	T13355	T13356	nummod	×,104
R10489	T13356	T13348	appos	104,upregulation
R10490	T13357	T13356	prep	in,104
R10491	T13358	T13359	nummod	2,ml
R10492	T13359	T13357	pobj	ml,in
R10493	T13360	T13348	punct	),upregulation
R10494	T13361	T13361	ROOT	were,were
R10495	T13362	T13361	acomp	cultured,were
R10496	T13363	T13362	prep	in,cultured
R10497	T13364	T13365	amod	24-well,plates
R10498	T13365	T13363	pobj	plates,in
R10499	T13366	T13361	prep	for,were
R10500	T13367	T13368	nummod	3,days
R10501	T13368	T13366	pobj	days,for
R10502	T13369	T13361	prep	with,were
R10503	T13370	T13371	nummod	5,µg
R10504	T13371	T13374	amod	µg,anti-CD40
R10505	T13372	T13374	nmod	/,anti-CD40
R10506	T13373	T13374	compound	ml,anti-CD40
R10507	T13374	T13369	pobj	anti-CD40,with
R10508	T13375	T13374	cc	or,anti-CD40
R10509	T13376	T13379	det	an,antibody
R10510	T13377	T13379	amod	isotype,antibody
R10511	T13378	T13379	compound	control,antibody
R10512	T13379	T13374	conj	antibody,anti-CD40
R10513	T13380	T13361	punct	.,were
R10514	T13381	T13383	nsubjpass	Cells,stained
R10515	T13382	T13383	auxpass	were,stained
R10516	T13383	T13383	ROOT	stained,stained
R10517	T13384	T13383	prep	for,stained
R10518	T13385	T13384	pobj	flow,for
R10519	T13386	T13383	conj	cytometry,stained
R10520	T13387	T13386	prep	with,cytometry
R10521	T13388	T13387	pobj	FITC-anti-CD80,with
R10522	T13389	T13388	cc	or,FITC-anti-CD80
R10523	T13390	T13393	det	an,antibody
R10524	T13391	T13393	amod	appropriate,antibody
R10525	T13392	T13393	compound	control,antibody
R10526	T13393	T13388	conj	antibody,FITC-anti-CD80
R10527	T13394	T13395	mark	as,described
R10528	T13395	T13383	advcl	described,stained
R10529	T13396	T13398	nmod	[,]
R10530	T13397	T13398	nummod	28,]
R10531	T13398	T13395	dobj	],described
R10532	T13399	T13383	punct	.,stained
R10533	T13400	T13401	nsubj	Data,acquired
R10534	T13401	T13401	ROOT	acquired,acquired
R10535	T13402	T13401	prep	with,acquired
R10536	T13403	T13405	det	a,flow
R10537	T13404	T13405	amod	FACScan,flow
R10538	T13405	T13406	compound	flow,cytometer
R10539	T13406	T13402	pobj	cytometer,with
R10540	T13407	T13409	punct	(,Biosciences
R10541	T13408	T13409	compound	BD,Biosciences
R10542	T13409	T13406	appos	Biosciences,cytometer
R10543	T13410	T13401	punct	),acquired
R10544	T13411	T13412	auxpass	were,analyzed
R10545	T13412	T13401	conj	analyzed,acquired
R10546	T13413	T13412	prep	with,analyzed
R10547	T13414	T13413	pobj	WinMDI,with
R10548	T13415	T13414	nummod	2.8,WinMDI
R10549	T13416	T13419	punct	(,Institute
R10550	T13417	T13419	compound	Scripps,Institute
R10551	T13418	T13419	compound	Research,Institute
R10552	T13419	T13419	ROOT	Institute,Institute
R10553	T13420	T13419	punct	",",Institute
R10554	T13421	T13422	compound	San,Diego
R10555	T13422	T13419	npadvmod	Diego,Institute
R10556	T13423	T13422	punct	",",Diego
R10557	T13424	T13422	appos	CA,Diego
R10558	T13425	T13419	punct	),Institute
R10559	T13426	T13419	punct	.,Institute
R10653	T13569	T13579	nsubjpass	GLε,evaluated
R10654	T13570	T13571	compound	transcript,assay
R10655	T13571	T13569	dobj	assay,GLε
R10656	T13572	T13574	det	The,activation
R10657	T13573	T13574	amod	CD40-simulated,activation
R10658	T13574	T13579	nsubjpass	activation,evaluated
R10659	T13575	T13574	prep	of,activation
R10660	T13576	T13577	compound	GLε,transcription
R10661	T13577	T13575	pobj	transcription,of
R10662	T13578	T13579	auxpass	was,evaluated
R10663	T13579	T13579	ROOT	evaluated,evaluated
R10664	T13580	T13582	mark	as,reported
R10665	T13581	T13582	advmod	previously,reported
R10666	T13582	T13579	advcl	reported,evaluated
R10667	T13583	T13585	nmod	[,]
R10668	T13584	T13585	nummod	33,]
R10669	T13585	T13582	nsubj	],reported
R10670	T13586	T13579	punct	.,evaluated
R10671	T13587	T13594	advmod	Briefly,stimulated
R10672	T13588	T13594	punct	",",stimulated
R10673	T13589	T13592	nummod	1,cells
R10674	T13590	T13592	nummod	×,cells
R10675	T13591	T13592	nummod	106,cells
R10676	T13592	T13594	nsubjpass	cells,stimulated
R10677	T13593	T13594	auxpass	were,stimulated
R10678	T13594	T13594	ROOT	stimulated,stimulated
R10679	T13595	T13594	advmod	overnight,stimulated
R10680	T13596	T13594	prep	with,stimulated
R10681	T13597	T13598	amod	anti-CD40,antibody
R10682	T13598	T13596	pobj	antibody,with
R10683	T13599	T13598	punct	(,antibody
R10684	T13600	T13601	nummod	10,µg
R10685	T13601	T13603	compound	µg,ml
R10686	T13602	T13603	compound	/,ml
R10687	T13603	T13598	appos	ml,antibody
R10688	T13604	T13598	punct	),antibody
R10689	T13605	T13598	cc	or,antibody
R10690	T13606	T13609	det	an,antibody
R10691	T13607	T13609	compound	isotype,antibody
R10692	T13608	T13609	compound	control,antibody
R10693	T13609	T13598	conj	antibody,antibody
R10694	T13610	T13596	punct	",",with
R10695	T13611	T13596	conj	with,with
R10696	T13612	T13611	cc	or,with
R10697	T13613	T13611	conj	without,with
R10698	T13614	T13617	nummod	500,IL-4
R10699	T13615	T13617	compound	U/ml,IL-4
R10700	T13616	T13617	compound	mouse,IL-4
R10701	T13617	T13613	pobj	IL-4,without
R10702	T13618	T13620	punct	(,Biosciences
R10703	T13619	T13620	compound	BD,Biosciences
R10704	T13620	T13617	appos	Biosciences,IL-4
R10705	T13621	T13620	punct	),Biosciences
R10706	T13622	T13594	punct	.,stimulated
R10707	T13623	T13625	nsubjpass	RNA,isolated
R10708	T13624	T13625	auxpass	was,isolated
R10709	T13625	T13625	ROOT	isolated,isolated
R10710	T13626	T13625	advcl	using,isolated
R10711	T13627	T13626	dobj	Trizol,using
R10712	T13628	T13629	punct	(,Invitrogen
R10713	T13629	T13627	appos	Invitrogen,Trizol
R10714	T13630	T13629	punct	),Invitrogen
R10715	T13631	T13627	punct	",",Trizol
R10716	T13632	T13627	cc	and,Trizol
R10717	T13633	T13627	conj	reverse-transcribed,Trizol
R10718	T13634	T13633	punct	(,reverse-transcribed
R10719	T13635	T13636	compound	Superscript,III
R10720	T13636	T13637	compound	III,kit
R10721	T13637	T13633	appos	kit,reverse-transcribed
R10722	T13638	T13637	punct	",",kit
R10723	T13639	T13637	appos	Invitrogen,kit
R10724	T13640	T13633	punct	),reverse-transcribed
R10725	T13641	T13625	punct	.,isolated
R10726	T13642	T13643	compound	Quantitative,PCR
R10727	T13643	T13649	nsubjpass	PCR,performed
R10728	T13644	T13643	prep	for,PCR
R10729	T13645	T13644	pobj	GLε,for
R10730	T13646	T13645	cc	and,GLε
R10731	T13647	T13645	conj	Hprt1,GLε
R10732	T13648	T13649	auxpass	was,performed
R10733	T13649	T13649	ROOT	performed,performed
R10734	T13650	T13649	advcl	using,performed
R10735	T13651	T13654	compound	SYBR,mix
R10736	T13652	T13654	compound	GREEN,mix
R10737	T13653	T13654	compound	master,mix
R10738	T13654	T13650	dobj	mix,using
R10739	T13655	T13657	punct	(,Biosystems
R10740	T13656	T13657	compound	Applied,Biosystems
R10741	T13657	T13654	appos	Biosystems,mix
R10742	T13658	T13654	punct	),mix
R10743	T13659	T13649	punct	",",performed
R10744	T13660	T13649	cc	and,performed
R10745	T13661	T13668	det	an,instrument
R10746	T13662	T13665	compound	Applied,Fast
R10747	T13663	T13665	compound	Biosystems,Fast
R10748	T13664	T13665	compound	7900HT,Fast
R10749	T13665	T13667	compound	Fast,PCR
R10750	T13666	T13667	compound	Real-Time,PCR
R10751	T13667	T13668	compound	PCR,instrument
R10752	T13668	T13649	conj	instrument,performed
R10753	T13669	T13649	punct	.,performed
R10754	T13670	T13676	nsubj	Expression,was
R10755	T13671	T13670	prep	of,Expression
R10756	T13672	T13671	pobj	GLε,of
R10757	T13673	T13670	prep	in,Expression
R10758	T13674	T13675	det	each,sample
R10759	T13675	T13673	pobj	sample,in
R10760	T13676	T13676	ROOT	was,was
R10761	T13677	T13676	acomp	normalized,was
R10762	T13678	T13677	prep	to,normalized
R10763	T13679	T13680	det	the,expression
R10764	T13680	T13678	pobj	expression,to
R10765	T13681	T13680	prep	of,expression
R10766	T13682	T13681	pobj	Hprt1,of
R10767	T13683	T13676	punct	.,was
R10900	T13876	T13880	nmod	NF-κB,assays
R10901	T13877	T13876	cc	and,NF-κB
R10902	T13878	T13876	conj	JNK,NF-κB
R10903	T13879	T13880	compound	activation,assays
R10904	T13880	T13905	nsubjpass	assays,added
R10905	T13881	T13880	prep	For,assays
R10906	T13882	T13881	pobj	activation,For
R10907	T13883	T13882	prep	of,activation
R10908	T13884	T13883	pobj	CD40,of
R10909	T13885	T13881	pobj	signaling,For
R10910	T13886	T13885	punct	",",signaling
R10911	T13887	T13888	nummod	5,×
R10912	T13888	T13892	dep	×,cells
R10913	T13889	T13892	nummod	104,cells
R10914	T13890	T13892	nummod	HI5,cells
R10915	T13891	T13892	compound	insect,cells
R10916	T13892	T13880	appos	cells,assays
R10917	T13893	T13892	cc	or,cells
R10918	T13894	T13896	nummod	HI5,cells
R10919	T13895	T13896	compound	insect,cells
R10920	T13896	T13892	conj	cells,cells
R10921	T13897	T13896	acl	expressing,cells
R10922	T13898	T13899	compound	mouse,CD154
R10923	T13899	T13897	dobj	CD154,expressing
R10924	T13900	T13899	punct	(,CD154
R10925	T13901	T13902	compound	CD40,ligand
R10926	T13902	T13899	appos	ligand,CD154
R10927	T13903	T13899	punct	),CD154
R10928	T13904	T13905	auxpass	were,added
R10929	T13905	T13905	ROOT	added,added
R10930	T13906	T13905	prep	to,added
R10931	T13907	T13911	nummod	1,cells
R10932	T13908	T13911	nummod	×,cells
R10933	T13909	T13911	nummod	106,cells
R10934	T13910	T13911	compound	B,cells
R10935	T13911	T13906	pobj	cells,to
R10936	T13912	T13905	punct	.,added
R10937	T13913	T13915	nsubjpass	Cells,centrifuged
R10938	T13914	T13915	auxpass	were,centrifuged
R10939	T13915	T13915	ROOT	centrifuged,centrifuged
R10940	T13916	T13915	prep	for,centrifuged
R10941	T13917	T13918	nummod	1,minute
R10942	T13918	T13916	pobj	minute,for
R10943	T13919	T13915	prep	at,centrifuged
R10944	T13920	T13922	nummod	400,g
R10945	T13921	T13922	punct	x,g
R10946	T13922	T13919	pobj	g,at
R10947	T13923	T13922	punct	(,g
R10948	T13924	T13925	aux	to,promote
R10949	T13925	T13922	acl	promote,g
R10950	T13926	T13925	dobj	contact,promote
R10951	T13927	T13926	prep	between,contact
R10952	T13928	T13929	amod	ligand,cells
R10953	T13929	T13927	pobj	cells,between
R10954	T13930	T13929	cc	and,cells
R10955	T13931	T13932	compound	B,cells
R10956	T13932	T13929	conj	cells,cells
R10957	T13933	T13925	punct	),promote
R10958	T13934	T13935	advmod	then,incubated
R10959	T13935	T13922	dep	incubated,g
R10960	T13936	T13935	prep	at,incubated
R10961	T13937	T13938	nummod	37,°
R10962	T13938	T13939	nummod	°,C
R10963	T13939	T13936	pobj	C,at
R10964	T13940	T13935	prep	for,incubated
R10965	T13941	T13940	pobj	times,for
R10966	T13942	T13935	conj	indicated,incubated
R10967	T13943	T13942	prep	in,indicated
R10968	T13944	T13943	pobj	Fig.,in
R10969	T13945	T13944	nummod	4,Fig.
R10970	T13946	T13915	punct	.,centrifuged
R10971	T13947	T13952	prep	Following,chilled
R10972	T13948	T13947	pobj	stimulation,Following
R10973	T13949	T13952	punct	",",chilled
R10974	T13950	T13952	nsubjpass	cells,chilled
R10975	T13951	T13952	auxpass	were,chilled
R10976	T13952	T13952	ROOT	chilled,chilled
R10977	T13953	T13952	prep	on,chilled
R10978	T13954	T13953	pobj	ice,on
R10979	T13955	T13952	prep	for,chilled
R10980	T13956	T13957	nummod	2,minutes
R10981	T13957	T13955	pobj	minutes,for
R10982	T13958	T13952	punct	.,chilled
R10983	T13959	T13960	compound	Cell,pellets
R10984	T13960	T13962	nsubjpass	pellets,dissolved
R10985	T13961	T13962	auxpass	were,dissolved
R10986	T13962	T13962	ROOT	dissolved,dissolved
R10987	T13963	T13962	prep	in,dissolved
R10988	T13964	T13965	compound	2X,SDS-PAGE
R10989	T13965	T13967	compound	SDS-PAGE,buffer
R10990	T13966	T13967	compound	loading,buffer
R10991	T13967	T13963	pobj	buffer,in
R10992	T13968	T13962	punct	",",dissolved
R10993	T13969	T13962	advcl	sonicated,dissolved
R10994	T13970	T13969	punct	",",sonicated
R10995	T13971	T13969	cc	and,sonicated
R10996	T13972	T13969	conj	heated,sonicated
R10997	T13973	T13972	prep	for,heated
R10998	T13974	T13975	nummod	5,minutes
R10999	T13975	T13973	pobj	minutes,for
R11000	T13976	T13975	prep	at,minutes
R11001	T13977	T13978	nummod	95,°
R11002	T13978	T13982	nummod	°,lysates
R11003	T13979	T13980	compound	C.,Total
R11004	T13980	T13981	compound	Total,cell
R11005	T13981	T13982	compound	cell,lysates
R11006	T13982	T13976	pobj	lysates,at
R11007	T13983	T13982	punct	(,lysates
R11008	T13984	T13988	nummod	1,equivalents
R11009	T13985	T13988	nummod	×,equivalents
R11010	T13986	T13988	nummod	105,equivalents
R11011	T13987	T13988	compound	cell,equivalents
R11012	T13988	T13982	appos	equivalents,lysates
R11013	T13989	T13988	prep	per,equivalents
R11014	T13990	T13989	pobj	lane,per
R11015	T13991	T13962	punct	),dissolved
R11016	T13992	T13993	auxpass	were,fractionated
R11017	T13993	T13993	ROOT	fractionated,fractionated
R11018	T13994	T13993	agent	by,fractionated
R11019	T13995	T13994	pobj	SDS-PAGE,by
R11020	T13996	T13993	cc	and,fractionated
R11021	T13997	T13993	conj	blotted,fractionated
R11022	T13998	T13997	prep	to,blotted
R11023	T13999	T14000	compound	polyvinylidine,fluoride
R11024	T14000	T13998	pobj	fluoride,to
R11025	T14001	T14000	punct	(,fluoride
R11026	T14002	T14000	appos	PVDF,fluoride
R11027	T14003	T14000	punct	),fluoride
R11028	T14004	T13993	nsubjpass	membranes,fractionated
R11029	T14005	T13993	punct	.,fractionated
R11030	T14006	T14008	nsubjpass	Membranes,probed
R11031	T14007	T14008	auxpass	were,probed
R11032	T14008	T14008	ROOT	probed,probed
R11033	T14009	T14008	prep	with,probed
R11034	T14010	T14009	pobj	antibodies,with
R11035	T14011	T14012	mark	as,indicated
R11036	T14012	T14008	advcl	indicated,probed
R11037	T14013	T14012	prep	in,indicated
R11038	T14014	T14013	pobj	Fig.,in
R11039	T14015	T14014	nummod	4,Fig.
R11040	T14016	T14008	punct	.,probed
R11041	T14017	T14018	amod	Chemiluminescent,detection
R11042	T14018	T14018	ROOT	detection,detection
R11043	T14019	T14024	punct	(,used
R11044	T14020	T14021	compound	Pierce,Biotechnology
R11045	T14021	T14024	nsubjpass	Biotechnology,used
R11046	T14022	T14021	punct	),Biotechnology
R11047	T14023	T14024	auxpass	was,used
R11048	T14024	T14024	ROOT	used,used
R11049	T14025	T14024	prep	for,used
R11050	T14026	T14027	det	the,visualization
R11051	T14027	T14025	pobj	visualization,for
R11052	T14028	T14027	prep	of,visualization
R11053	T14029	T14028	pobj	bands,of
R11054	T14030	T14029	prep	on,bands
R11055	T14031	T14032	amod	Western,blots
R11056	T14032	T14030	pobj	blots,on
R11057	T14033	T14024	punct	.,used
R11058	T14034	T14038	nsubjpass	Images,recorded
R11059	T14035	T14034	prep	of,Images
R11060	T14036	T14035	pobj	blots,of
R11061	T14037	T14038	auxpass	were,recorded
R11062	T14038	T14038	ROOT	recorded,recorded
R11063	T14039	T14038	prep	with,recorded
R11064	T14040	T14043	det	a,system
R11065	T14041	T14043	amod	low-light,system
R11066	T14042	T14043	compound	imaging,system
R11067	T14043	T14039	pobj	system,with
R11068	T14044	T14043	punct	(,system
R11069	T14045	T14043	appos	LAS4000,system
R11070	T14046	T14043	punct	",",system
R11071	T14047	T14049	compound	Fuji,Systems
R11072	T14048	T14049	compound	Medical,Systems
R11073	T14049	T14043	appos	Systems,system
R11074	T14050	T14043	punct	),system
R11075	T14051	T14039	cc	and,with
R11076	T14052	T14039	conj	on,with
R11077	T14053	T14054	compound	X-ray,film
R11078	T14054	T14052	pobj	film,on
R11079	T14055	T14038	punct	.,recorded
R11257	T14284	T14285	compound	Immunoprecipitations,Immunoprecipitation
R11258	T14285	T14289	nsubjpass	Immunoprecipitation,performed
R11259	T14286	T14285	prep	of,Immunoprecipitation
R11260	T14287	T14286	pobj	CD40,of
R11261	T14288	T14289	auxpass	was,performed
R11262	T14289	T14289	ROOT	performed,performed
R11263	T14290	T14289	agent	by,performed
R11264	T14291	T14293	amod	activated,capture
R11265	T14292	T14293	compound	receptor,capture
R11266	T14293	T14290	pobj	capture,by
R11267	T14294	T14289	punct	",",performed
R11268	T14295	T14297	mark	as,described
R11269	T14296	T14297	advmod	previously,described
R11270	T14297	T14289	advcl	described,performed
R11271	T14298	T14300	nmod	[,]
R11272	T14299	T14300	nummod	6,]
R11273	T14300	T14297	dobj	],described
R11274	T14301	T14289	punct	.,performed
R11275	T14302	T14316	advmod	Briefly,incubated
R11276	T14303	T14316	punct	",",incubated
R11277	T14304	T14305	nummod	3,×
R11278	T14305	T14316	nsubjpass	×,incubated
R11279	T14306	T14307	nummod	107,cells
R11280	T14307	T14305	appos	cells,×
R11281	T14308	T14307	punct	(,cells
R11282	T14309	T14311	nummod	A20,J
R11283	T14310	T14311	nummod	.2,J
R11284	T14311	T14307	appos	J,cells
R11285	T14312	T14311	cc	and,J
R11286	T14313	T14311	conj	derivatives,J
R11287	T14314	T14307	punct	),cells
R11288	T14315	T14316	auxpass	were,incubated
R11289	T14316	T14316	ROOT	incubated,incubated
R11290	T14317	T14316	prep	for,incubated
R11291	T14318	T14319	nummod	60,minutes
R11292	T14319	T14317	pobj	minutes,for
R11293	T14320	T14319	prep	at,minutes
R11294	T14321	T14322	compound	room,temperature
R11295	T14322	T14320	pobj	temperature,at
R11296	T14323	T14316	prep	with,incubated
R11297	T14324	T14325	nummod	10,µl
R11298	T14325	T14329	compound	µl,beads
R11299	T14326	T14327	amod	magnetic,protein
R11300	T14327	T14329	compound	protein,beads
R11301	T14328	T14329	compound	G,beads
R11302	T14329	T14323	pobj	beads,with
R11303	T14330	T14329	punct	(,beads
R11304	T14331	T14329	appos	Dynal,beads
R11305	T14332	T14329	punct	),beads
R11306	T14333	T14316	advcl	pre-coated,incubated
R11307	T14334	T14333	prep	with,pre-coated
R11308	T14335	T14336	nummod	10,µg
R11309	T14336	T14339	compound	µg,IgG
R11310	T14337	T14339	compound	goat,IgG
R11311	T14338	T14339	compound	anti-rat,IgG
R11312	T14339	T14334	pobj	IgG,with
R11313	T14340	T14341	punct	(,Jackson
R11314	T14341	T14339	appos	Jackson,IgG
R11315	T14342	T14341	punct	),Jackson
R11316	T14343	T14339	punct	",",IgG
R11317	T14344	T14339	cc	and,IgG
R11318	T14345	T14347	nummod	10,anti-CD40
R11319	T14346	T14347	compound	µg,anti-CD40
R11320	T14347	T14339	conj	anti-CD40,IgG
R11321	T14348	T14349	punct	(,1C10
R11322	T14349	T14347	appos	1C10,anti-CD40
R11323	T14350	T14349	punct	),1C10
R11324	T14351	T14347	cc	or,anti-CD40
R11325	T14352	T14355	det	an,antibody
R11326	T14353	T14355	nmod	isotype,antibody
R11327	T14354	T14355	compound	control,antibody
R11328	T14355	T14347	conj	antibody,anti-CD40
R11329	T14356	T14355	punct	(,antibody
R11330	T14357	T14355	appos	mAb72,antibody
R11331	T14358	T14355	punct	),antibody
R11332	T14359	T14316	punct	.,incubated
R11333	T14360	T14363	nsubjpass	Cells/beads,pelleted
R11334	T14361	T14363	auxpass	were,pelleted
R11335	T14362	T14363	advmod	then,pelleted
R11336	T14363	T14363	ROOT	pelleted,pelleted
R11337	T14364	T14363	agent	by,pelleted
R11338	T14365	T14364	pobj	centrifugation,by
R11339	T14366	T14363	cc	and,pelleted
R11340	T14367	T14363	conj	lysed,pelleted
R11341	T14368	T14367	prep	in,lysed
R11342	T14369	T14368	pobj	buffer,in
R11343	T14370	T14369	acl	containing,buffer
R11344	T14371	T14372	nummod	1,%
R11345	T14372	T14374	compound	%,X100
R11346	T14373	T14374	compound	Triton,X100
R11347	T14374	T14370	dobj	X100,containing
R11348	T14375	T14363	punct	.,pelleted
R11349	T14376	T14378	nsubjpass	Beads,washed
R11350	T14377	T14378	auxpass	were,washed
R11351	T14378	T14378	ROOT	washed,washed
R11352	T14379	T14378	prep	with,washed
R11353	T14380	T14381	compound	lysis,buffer
R11354	T14381	T14379	pobj	buffer,with
R11355	T14382	T14383	aux	to,remove
R11356	T14383	T14378	advcl	remove,washed
R11357	T14384	T14385	amod	unbound,material
R11358	T14385	T14383	dobj	material,remove
R11359	T14386	T14378	punct	.,washed
R11360	T14387	T14397	prep	In,dephosphorylated
R11361	T14388	T14389	det	some,experiments
R11362	T14389	T14387	pobj	experiments,In
R11363	T14390	T14397	punct	",",dephosphorylated
R11364	T14391	T14396	nsubj	material,was
R11365	T14392	T14391	acl	associated,material
R11366	T14393	T14392	prep	with,associated
R11367	T14394	T14395	det	the,beads
R11368	T14395	T14393	pobj	beads,with
R11369	T14396	T14397	auxpass	was,dephosphorylated
R11370	T14397	T14397	ROOT	dephosphorylated,dephosphorylated
R11371	T14398	T14397	prep	with,dephosphorylated
R11372	T14399	T14400	compound	lambda,phosphatase
R11373	T14400	T14398	pobj	phosphatase,with
R11374	T14401	T14404	punct	(,BioLabs
R11375	T14402	T14404	compound	New,BioLabs
R11376	T14403	T14404	compound	England,BioLabs
R11377	T14404	T14400	appos	BioLabs,phosphatase
R11378	T14405	T14404	punct	),BioLabs
R11379	T14406	T14397	prep	as,dephosphorylated
R11380	T14407	T14406	prep	per,as
R11381	T14408	T14410	poss	manufacturer,instructions
R11382	T14409	T14408	case	's,manufacturer
R11383	T14410	T14407	pobj	instructions,per
R11384	T14411	T14397	punct	.,dephosphorylated
R11385	T14412	T14414	nsubjpass	Beads,resuspended
R11386	T14413	T14414	auxpass	were,resuspended
R11387	T14414	T14414	ROOT	resuspended,resuspended
R11388	T14415	T14414	prep	in,resuspended
R11389	T14416	T14417	compound	2X,SDS-PAGE
R11390	T14417	T14419	compound	SDS-PAGE,buffer
R11391	T14418	T14419	compound	sample,buffer
R11392	T14419	T14415	pobj	buffer,in
R11393	T14420	T14414	cc	and,resuspended
R11394	T14421	T14414	conj	heated,resuspended
R11395	T14422	T14421	prep	for,heated
R11396	T14423	T14424	nummod	5,minutes
R11397	T14424	T14422	pobj	minutes,for
R11398	T14425	T14424	prep	at,minutes
R11399	T14426	T14430	nummod	95,eluted
R11400	T14427	T14430	nummod	°,eluted
R11401	T14428	T14429	compound	C.,Material
R11402	T14429	T14430	compound	Material,eluted
R11403	T14430	T14414	conj	eluted,resuspended
R11404	T14431	T14430	prep	from,eluted
R11405	T14432	T14433	det	the,beads
R11406	T14433	T14431	pobj	beads,from
R11407	T14434	T14435	auxpass	was,fractionated
R11408	T14435	T14435	ROOT	fractionated,fractionated
R11409	T14436	T14435	agent	by,fractionated
R11410	T14437	T14436	pobj	SDS-PAGE,by
R11411	T14438	T14435	cc	and,fractionated
R11412	T14439	T14435	conj	transferred,fractionated
R11413	T14440	T14439	prep	to,transferred
R11414	T14441	T14442	compound	PVDF,membranes
R11415	T14442	T14440	pobj	membranes,to
R11416	T14443	T14442	prep	for,membranes
R11417	T14444	T14445	amod	Western,blotting
R11418	T14445	T14443	pobj	blotting,for
R11419	T14446	T14435	punct	.,fractionated
R11420	T14447	T14453	prep	In,cultured
R11421	T14448	T14449	det	some,experiments
R11422	T14449	T14447	pobj	experiments,In
R11423	T14450	T14453	punct	",",cultured
R11424	T14451	T14453	nsubjpass	cells,cultured
R11425	T14452	T14453	auxpass	were,cultured
R11426	T14453	T14453	ROOT	cultured,cultured
R11427	T14454	T14453	prep	for,cultured
R11428	T14455	T14456	nummod	6,hrs
R11429	T14456	T14454	pobj	hrs,for
R11430	T14457	T14456	prep	with,hrs
R11431	T14458	T14459	nummod	25,µM
R11432	T14459	T14462	nmod	µM,peptide
R11433	T14460	T14462	amod	antennapedia-linked,peptide
R11434	T14461	T14462	compound	SMAC-N7,peptide
R11435	T14462	T14457	pobj	peptide,with
R11436	T14463	T14462	punct	(,peptide
R11437	T14464	T14462	appos	Calbiochem,peptide
R11438	T14465	T14462	punct	),peptide
R11439	T14466	T14462	cc	or,peptide
R11440	T14467	T14469	det	an,volume
R11441	T14468	T14469	amod	appropriate,volume
R11442	T14469	T14453	dobj	volume,cultured
R11443	T14470	T14469	prep	of,volume
R11444	T14471	T14472	det	the,solvent
R11445	T14472	T14470	pobj	solvent,of
R11446	T14473	T14469	acl	used,volume
R11447	T14474	T14473	prep	for,used
R11448	T14475	T14476	det	the,peptide
R11449	T14476	T14474	pobj	peptide,for
R11450	T14477	T14476	punct	(,peptide
R11451	T14478	T14476	appos	DMSO,peptide
R11452	T14479	T14476	punct	),peptide
R11453	T14480	T14453	punct	.,cultured
R11454	T14481	T14494	prep	After,stimulated
R11455	T14482	T14481	pobj	incubation,After
R11456	T14483	T14494	punct	",",stimulated
R11457	T14484	T14494	nsubjpass	cells,stimulated
R11458	T14485	T14484	punct	(,cells
R11459	T14486	T14484	prep	in,cells
R11460	T14487	T14486	pobj	peptide,in
R11461	T14488	T14486	punct	-,in
R11462	T14489	T14486	cc	or,in
R11463	T14490	T14491	amod	DMSO-containing,medium
R11464	T14491	T14484	conj	medium,cells
R11465	T14492	T14484	punct	),cells
R11466	T14493	T14494	auxpass	were,stimulated
R11467	T14494	T14494	ROOT	stimulated,stimulated
R11468	T14495	T14494	prep	with,stimulated
R11469	T14496	T14497	amod	antibody-coated,beads
R11470	T14497	T14495	pobj	beads,with
R11471	T14498	T14499	mark	as,outlined
R11472	T14499	T14494	advcl	outlined,stimulated
R11473	T14500	T14499	prep	above,outlined
R11474	T14501	T14494	punct	.,stimulated

GO-BP

Id	Subject	Object	Predicate	Lexical cue
T12839	1701-1708	http://purl.obolibrary.org/obo/GO_0051923	denotes	sulfate
T13298	2418-2430	http://purl.obolibrary.org/obo/GO_0009293	denotes	transduction
T13299	2482-2494	http://purl.obolibrary.org/obo/GO_0009293	denotes	transduction
T13507	3852-3854	http://purl.obolibrary.org/obo/GO_0033968	denotes	CA
T13803	3916-3929	http://purl.obolibrary.org/obo/GO_0006351	denotes	transcription
T14231	4494-4497	http://purl.obolibrary.org/obo/GO_0004705	denotes	JNK
T14232	4539-4548	http://purl.obolibrary.org/obo/GO_0023052	denotes	signaling
T14717	5906-5911	http://purl.obolibrary.org/obo/GO_0019835	denotes	lysis
T14718	6036-6047	http://purl.obolibrary.org/obo/GO_0016791	denotes	phosphatase

GO-MF

Id	Subject	Object	Predicate	Lexical cue
T12281	304-312	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T12282	383-391	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T12283	836-846	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies
T13508	3599-3607	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T13509	3693-3701	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T13510	3852-3854	http://purl.obolibrary.org/obo/GO_0033968	denotes	CA
T13804	4035-4043	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T13805	4077-4085	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T13806	4118-4122	http://purl.obolibrary.org/obo/GO_0005136	denotes	IL-4
T14233	4494-4497	http://purl.obolibrary.org/obo/GO_0004705	denotes	JNK
T14234	4622-4628	http://purl.obolibrary.org/obo/GO_0005488	denotes	ligand
T14235	4734-4740	http://purl.obolibrary.org/obo/GO_0005488	denotes	ligand
T14236	5149-5159	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibodies
T14719	5769-5777	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T14720	6573-6581	http://purl.obolibrary.org/obo/GO_0003823	denotes	antibody
T14721	6036-6047	http://purl.obolibrary.org/obo/GO_0016791	denotes	phosphatase

GO-CC

Id	Subject	Object	Predicate	Lexical cue
T12840	892-896	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T12841	945-950	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T12842	1574-1579	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T14242	4837-4842	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T14243	4878-4882	http://purl.obolibrary.org/obo/GO_0005623	denotes	Cell
T14244	4988-4992	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T14245	5008-5012	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T14246	5111-5120	http://purl.obolibrary.org/obo/GO_0016020	denotes	membranes
T14247	5122-5131	http://purl.obolibrary.org/obo/GO_0016020	denotes	Membranes
T14248	5149-5159	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T14249	5149-5159	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies
T14728	6573-6581	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T14729	6276-6285	http://purl.obolibrary.org/obo/GO_0016020	denotes	membranes
T14722	5544-5549	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T14723	6329-6334	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T14724	6506-6511	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T14725	5769-5777	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T14726	6573-6581	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T14727	5769-5777	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T12043	23-27	http://purl.obolibrary.org/obo/GO_0005623	denotes	Cell
T12044	46-50	http://purl.obolibrary.org/obo/GO_0005623	denotes	cell
T12045	204-209	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T12284	304-312	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T12285	383-391	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T12286	836-846	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibodies
T12287	304-312	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T12288	383-391	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T12289	836-846	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibodies
T13300	2498-2503	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T13301	2957-2962	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T13302	3141-3146	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T13303	3185-3190	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T13304	3409-3414	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T13511	3599-3607	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T13512	3693-3701	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T13513	3599-3607	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T13514	3693-3701	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T14237	4567-4572	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T14238	4587-4592	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T14239	4652-4657	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T14240	4741-4746	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T14241	4753-4758	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T13807	3988-3993	http://purl.obolibrary.org/obo/GO_0005623	denotes	cells
T13808	4035-4043	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T13809	4077-4085	http://purl.obolibrary.org/obo/GO_0019815	denotes	antibody
T13810	4035-4043	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody
T13811	4077-4085	http://purl.obolibrary.org/obo/GO_0042571	denotes	antibody

sentences

Id	Subject	Object	Predicate	Lexical cue
T11920	23-33	Sentence	denotes	Cell lines
T11921	34-104	Sentence	denotes	The mouse B cell line A20.2J has been previously described [26], [27].
T11922	105-191	Sentence	denotes	Cells were grown in RPMI 1640, 10% FCS, 10 µM 2-ME, 2 mM L-glutamine, and antibiotics.
T11923	192-265	Sentence	denotes	HI-5 insect cells (Invitrogen) expressing CD154 have been described [28].
T12054	267-277	Sentence	denotes	Antibodies
T12055	278-515	Sentence	denotes	Mouse anti-birch profilin antibody (4A6 [29]), rat anti-mouse CD40 (1C10 [30]) and a rat isotype control antibody (mAb72, Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA) were isolated from hybridoma supernatants.
T12056	516-594	Sentence	denotes	FITC-labeled anti-mouse CD80 and an isotype control Ab were from eBiosciences.
T12057	595-724	Sentence	denotes	Rabbit anti-HOIP Ab [6] was the kind gift of Dr. Betty Eipper (University of Connecticut Health Center, Farmington, Connecticut).
T12058	725-882	Sentence	denotes	Goat anti-rat IgG, and HRP-labeled secondary Abs were from Jackson ImmunoResearch Laboratories, Inc. All other antibodies used were described previously [6].
T12320	884-911	Sentence	denotes	Somatic cell gene targeting
T12321	912-1040	Sentence	denotes	The generation of HOIP-deficient cells was accomplished using a homologous recombination approach described previously [7], [8].
T12322	1041-1161	Sentence	denotes	Segments of Rnf31 gene sequence used in the targeting construct (Fig. 1A) were amplified by PCR from A20.2J genomic DNA.
T12323	1162-1312	Sentence	denotes	The oligonucleotide primers used to generate the 5′ flank (1224 bp) were 5′-ttttctagagcggtggcttaagtgaccc-3′ and 5′-tattctagatgcagcatctgagaaagcaagc-3′.
T12324	1313-1439	Sentence	denotes	The 3′ flank (6032 bp) primers were 5′-aaaaccggtgtatgcttctttacgggagaaaaatattag-3′ and 5′-tataccggtatgaagccaaaggaacactgagag-3′.
T12325	1440-1566	Sentence	denotes	Restriction endonuclease sites in the oligonucleotide primers allowed insertion of the PCR products into the targeting vector.
T12326	1567-1709	Sentence	denotes	A20.2J cells were transfected (by electroporation [7]) with the targeting construct and subcloned in medium containing 600 µg/ml G418 sulfate.
T12327	1710-1813	Sentence	denotes	Homologous recombination in G418-resistant clones was detected by PCR of genomic DNA, as described [7].
T12328	1814-2031	Sentence	denotes	Oligonucleotide primers used for screening were 5′-cttcctgatctcagctttaccgtcac-3′ (homologous to genomic sequence; approximate position noted in Fig. 1) and 5′-caatccatcttgttcagccat-3′ (homologous to sequence in NeoR).
T12329	2032-2179	Sentence	denotes	Clones in which one copy of Rnf31 had been disrupted were transiently transfected with an expression plasmid encoding Cre, in order to remove NeoR.
T12330	2180-2290	Sentence	denotes	G418-sensitive subclones were subjected to a second round of targeting to disrupt the remaining copy of Rnf31.
T12331	2291-2405	Sentence	denotes	G418-resistant clones were tested for homologous recombination by PCR and HOIP protein expression by Western blot.
T12902	2407-2430	Sentence	denotes	Retroviral transduction
T12903	2431-2694	Sentence	denotes	A retroviral vector (pMIP) was used for the stable transduction of cells with HOIP and IKKγ cDNA constructs. pMIP was constructed by replacing the IRES and GFP encoding sequences in pMIG [31] with the IRES and puromycin resistance gene from pIRESpuro2 (Clontech).
T12904	2695-2775	Sentence	denotes	FLAG-tagged mouse HOIP cDNA was inserted into the multiple cloning site of pMIP.
T12905	2776-2886	Sentence	denotes	A pMIP construct encoding mouse IKKγ with an amino terminal birch profilin peptide (BP)-tag was also prepared.
T12906	2887-3036	Sentence	denotes	Retroviral particles were generated by transient transfection of 293T cells with pMIP, pMIP-HOIP, or pMIP-IKKγ and the packaging vector pCL-Eco [32].
T12907	3037-3115	Sentence	denotes	Two days after transfection, culture supernatants were harvested and filtered.
T12908	3116-3258	Sentence	denotes	A20.2J or HOIP-deficient cells were added to 24-well plates (2.5×105 cells/well) with 1 ml virus-containing supernatant and 8 µg/ml polybrene.
T12909	3259-3324	Sentence	denotes	Plates were centrifuged at 500 x g for 2 hrs at room temperature.
T12910	3325-3450	Sentence	denotes	Cells were cultured in B cell culture medium for 48 hrs, after which the transduced cells were selected in 3 µg/ml puromycin.
T13343	3452-3488	Sentence	denotes	CD80 upregulation and flow cytometry
T13344	3489-3608	Sentence	denotes	Cells (5×104 in 2 ml) were cultured in 24-well plates for 3 days with 5 µg/ml anti-CD40 or an isotype control antibody.
T13345	3609-3720	Sentence	denotes	Cells were stained for flow cytometry with FITC-anti-CD80 or an appropriate control antibody as described [28].
T13346	3721-3856	Sentence	denotes	Data acquired with a FACScan flow cytometer (BD Biosciences) were analyzed with WinMDI 2.8 (Scripps Research Institute, San Diego, CA).
T13563	3858-3878	Sentence	denotes	GLε transcript assay
T13564	3879-3972	Sentence	denotes	The CD40-simulated activation of GLε transcription was evaluated as previously reported [33].
T13565	3973-4140	Sentence	denotes	Briefly, 1×106 cells were stimulated overnight with anti-CD40 antibody (10 µg/ml) or an isotype control antibody, with or without 500 U/ml mouse IL-4 (BD Biosciences).
T13566	4141-4243	Sentence	denotes	RNA was isolated using Trizol (Invitrogen), and reverse-transcribed (Superscript III kit, Invitrogen).
T13567	4244-4406	Sentence	denotes	Quantitative PCR for GLε and Hprt1 was performed using SYBR GREEN master mix (Applied Biosystems), and an Applied Biosystems 7900HT Fast Real-Time PCR instrument.
T13568	4407-4482	Sentence	denotes	Expression of GLε in each sample was normalized to the expression of Hprt1.
T13868	4484-4515	Sentence	denotes	NF-κB and JNK activation assays
T13869	4516-4658	Sentence	denotes	For activation of CD40 signaling, 5×104 HI5 insect cells or HI5 insect cells expressing mouse CD154 (CD40 ligand) were added to 1×106 B cells.
T13870	4659-4813	Sentence	denotes	Cells were centrifuged for 1 minute at 400 x g (to promote contact between ligand cells and B cells) then incubated at 37°C for times indicated in Fig. 4.
T13871	4814-4877	Sentence	denotes	Following stimulation, cells were chilled on ice for 2 minutes.
T13872	4878-5121	Sentence	denotes	Cell pellets were dissolved in 2X SDS-PAGE loading buffer, sonicated, and heated for 5 minutes at 95°C. Total cell lysates (1×105 cell equivalents per lane) were fractionated by SDS-PAGE and blotted to polyvinylidine fluoride (PVDF) membranes.
T13873	5122-5183	Sentence	denotes	Membranes were probed with antibodies as indicated in Fig. 4.
T13874	5184-5291	Sentence	denotes	Chemiluminescent detection (Pierce Biotechnology) was used for the visualization of bands on Western blots.
T13875	5292-5404	Sentence	denotes	Images of blots were recorded with a low-light imaging system (LAS4000, Fuji Medical Systems) and on X-ray film.
T14275	5406-5426	Sentence	denotes	Immunoprecipitations
T14276	5427-5528	Sentence	denotes	Immunoprecipitation of CD40 was performed by activated receptor capture, as previously described [6].
T14277	5529-5786	Sentence	denotes	Briefly, 3×107 cells (A20.2J and derivatives) were incubated for 60 minutes at room temperature with 10 µl magnetic protein G beads (Dynal) pre-coated with 10 µg goat anti-rat IgG (Jackson), and 10 µg anti-CD40 (1C10) or an isotype control antibody (mAb72).
T14278	5787-5882	Sentence	denotes	Cells/beads were then pelleted by centrifugation and lysed in buffer containing 1% Triton X100.
T14279	5883-5946	Sentence	denotes	Beads were washed with lysis buffer to remove unbound material.
T14280	5947-6105	Sentence	denotes	In some experiments, material associated with the beads was dephosphorylated with lambda phosphatase (New England BioLabs) as per manufacturer's instructions.
T14281	6106-6307	Sentence	denotes	Beads were resuspended in 2X SDS-PAGE sample buffer and heated for 5 minutes at 95°C. Material eluted from the beads was fractionated by SDS-PAGE and transferred to PVDF membranes for Western blotting.
T14282	6308-6487	Sentence	denotes	In some experiments, cells were cultured for 6 hrs with 25 µM antennapedia-linked SMAC-N7 peptide (Calbiochem) or an appropriate volume of the solvent used for the peptide (DMSO).
T14283	6488-6613	Sentence	denotes	After incubation, cells (in peptide- or DMSO-containing medium) were stimulated with antibody-coated beads as outlined above.
T180	0-21	Sentence	denotes	Materials and Methods
T181	23-33	Sentence	denotes	Cell lines
T182	34-104	Sentence	denotes	The mouse B cell line A20.2J has been previously described [26], [27].
T183	105-191	Sentence	denotes	Cells were grown in RPMI 1640, 10% FCS, 10 µM 2-ME, 2 mM L-glutamine, and antibiotics.
T184	192-265	Sentence	denotes	HI-5 insect cells (Invitrogen) expressing CD154 have been described [28].
T185	267-277	Sentence	denotes	Antibodies
T186	278-515	Sentence	denotes	Mouse anti-birch profilin antibody (4A6 [29]), rat anti-mouse CD40 (1C10 [30]) and a rat isotype control antibody (mAb72, Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA) were isolated from hybridoma supernatants.
T187	516-594	Sentence	denotes	FITC-labeled anti-mouse CD80 and an isotype control Ab were from eBiosciences.
T188	595-724	Sentence	denotes	Rabbit anti-HOIP Ab [6] was the kind gift of Dr. Betty Eipper (University of Connecticut Health Center, Farmington, Connecticut).
T189	725-825	Sentence	denotes	Goat anti-rat IgG, and HRP-labeled secondary Abs were from Jackson ImmunoResearch Laboratories, Inc.
T190	826-882	Sentence	denotes	All other antibodies used were described previously [6].
T191	884-911	Sentence	denotes	Somatic cell gene targeting
T192	912-1040	Sentence	denotes	The generation of HOIP-deficient cells was accomplished using a homologous recombination approach described previously [7], [8].
T193	1041-1161	Sentence	denotes	Segments of Rnf31 gene sequence used in the targeting construct (Fig. 1A) were amplified by PCR from A20.2J genomic DNA.
T194	1162-1312	Sentence	denotes	The oligonucleotide primers used to generate the 5′ flank (1224 bp) were 5′-ttttctagagcggtggcttaagtgaccc-3′ and 5′-tattctagatgcagcatctgagaaagcaagc-3′.
T195	1313-1439	Sentence	denotes	The 3′ flank (6032 bp) primers were 5′-aaaaccggtgtatgcttctttacgggagaaaaatattag-3′ and 5′-tataccggtatgaagccaaaggaacactgagag-3′.
T196	1440-1566	Sentence	denotes	Restriction endonuclease sites in the oligonucleotide primers allowed insertion of the PCR products into the targeting vector.
T197	1567-1709	Sentence	denotes	A20.2J cells were transfected (by electroporation [7]) with the targeting construct and subcloned in medium containing 600 µg/ml G418 sulfate.
T198	1710-1813	Sentence	denotes	Homologous recombination in G418-resistant clones was detected by PCR of genomic DNA, as described [7].
T199	1814-2031	Sentence	denotes	Oligonucleotide primers used for screening were 5′-cttcctgatctcagctttaccgtcac-3′ (homologous to genomic sequence; approximate position noted in Fig. 1) and 5′-caatccatcttgttcagccat-3′ (homologous to sequence in NeoR).
T200	2032-2179	Sentence	denotes	Clones in which one copy of Rnf31 had been disrupted were transiently transfected with an expression plasmid encoding Cre, in order to remove NeoR.
T201	2180-2290	Sentence	denotes	G418-sensitive subclones were subjected to a second round of targeting to disrupt the remaining copy of Rnf31.
T202	2291-2405	Sentence	denotes	G418-resistant clones were tested for homologous recombination by PCR and HOIP protein expression by Western blot.
T203	2407-2430	Sentence	denotes	Retroviral transduction
T204	2431-2694	Sentence	denotes	A retroviral vector (pMIP) was used for the stable transduction of cells with HOIP and IKKγ cDNA constructs. pMIP was constructed by replacing the IRES and GFP encoding sequences in pMIG [31] with the IRES and puromycin resistance gene from pIRESpuro2 (Clontech).
T205	2695-2775	Sentence	denotes	FLAG-tagged mouse HOIP cDNA was inserted into the multiple cloning site of pMIP.
T206	2776-2886	Sentence	denotes	A pMIP construct encoding mouse IKKγ with an amino terminal birch profilin peptide (BP)-tag was also prepared.
T207	2887-3036	Sentence	denotes	Retroviral particles were generated by transient transfection of 293T cells with pMIP, pMIP-HOIP, or pMIP-IKKγ and the packaging vector pCL-Eco [32].
T208	3037-3115	Sentence	denotes	Two days after transfection, culture supernatants were harvested and filtered.
T209	3116-3258	Sentence	denotes	A20.2J or HOIP-deficient cells were added to 24-well plates (2.5×105 cells/well) with 1 ml virus-containing supernatant and 8 µg/ml polybrene.
T210	3259-3324	Sentence	denotes	Plates were centrifuged at 500 x g for 2 hrs at room temperature.
T211	3325-3450	Sentence	denotes	Cells were cultured in B cell culture medium for 48 hrs, after which the transduced cells were selected in 3 µg/ml puromycin.
T212	3452-3488	Sentence	denotes	CD80 upregulation and flow cytometry
T213	3489-3608	Sentence	denotes	Cells (5×104 in 2 ml) were cultured in 24-well plates for 3 days with 5 µg/ml anti-CD40 or an isotype control antibody.
T214	3609-3720	Sentence	denotes	Cells were stained for flow cytometry with FITC-anti-CD80 or an appropriate control antibody as described [28].
T215	3721-3856	Sentence	denotes	Data acquired with a FACScan flow cytometer (BD Biosciences) were analyzed with WinMDI 2.8 (Scripps Research Institute, San Diego, CA).
T216	3858-3878	Sentence	denotes	GLε transcript assay
T217	3879-3972	Sentence	denotes	The CD40-simulated activation of GLε transcription was evaluated as previously reported [33].
T218	3973-4140	Sentence	denotes	Briefly, 1×106 cells were stimulated overnight with anti-CD40 antibody (10 µg/ml) or an isotype control antibody, with or without 500 U/ml mouse IL-4 (BD Biosciences).
T219	4141-4243	Sentence	denotes	RNA was isolated using Trizol (Invitrogen), and reverse-transcribed (Superscript III kit, Invitrogen).
T220	4244-4406	Sentence	denotes	Quantitative PCR for GLε and Hprt1 was performed using SYBR GREEN master mix (Applied Biosystems), and an Applied Biosystems 7900HT Fast Real-Time PCR instrument.
T221	4407-4482	Sentence	denotes	Expression of GLε in each sample was normalized to the expression of Hprt1.
T222	4484-4515	Sentence	denotes	NF-κB and JNK activation assays
T223	4516-4658	Sentence	denotes	For activation of CD40 signaling, 5×104 HI5 insect cells or HI5 insect cells expressing mouse CD154 (CD40 ligand) were added to 1×106 B cells.
T224	4659-4813	Sentence	denotes	Cells were centrifuged for 1 minute at 400 x g (to promote contact between ligand cells and B cells) then incubated at 37°C for times indicated in Fig. 4.
T225	4814-4877	Sentence	denotes	Following stimulation, cells were chilled on ice for 2 minutes.
T226	4878-4981	Sentence	denotes	Cell pellets were dissolved in 2X SDS-PAGE loading buffer, sonicated, and heated for 5 minutes at 95°C.
T227	4982-5121	Sentence	denotes	Total cell lysates (1×105 cell equivalents per lane) were fractionated by SDS-PAGE and blotted to polyvinylidine fluoride (PVDF) membranes.
T228	5122-5183	Sentence	denotes	Membranes were probed with antibodies as indicated in Fig. 4.
T229	5184-5291	Sentence	denotes	Chemiluminescent detection (Pierce Biotechnology) was used for the visualization of bands on Western blots.
T230	5292-5404	Sentence	denotes	Images of blots were recorded with a low-light imaging system (LAS4000, Fuji Medical Systems) and on X-ray film.
T231	5406-5426	Sentence	denotes	Immunoprecipitations
T232	5427-5528	Sentence	denotes	Immunoprecipitation of CD40 was performed by activated receptor capture, as previously described [6].
T233	5529-5786	Sentence	denotes	Briefly, 3×107 cells (A20.2J and derivatives) were incubated for 60 minutes at room temperature with 10 µl magnetic protein G beads (Dynal) pre-coated with 10 µg goat anti-rat IgG (Jackson), and 10 µg anti-CD40 (1C10) or an isotype control antibody (mAb72).
T234	5787-5882	Sentence	denotes	Cells/beads were then pelleted by centrifugation and lysed in buffer containing 1% Triton X100.
T235	5883-5946	Sentence	denotes	Beads were washed with lysis buffer to remove unbound material.
T236	5947-6105	Sentence	denotes	In some experiments, material associated with the beads was dephosphorylated with lambda phosphatase (New England BioLabs) as per manufacturer's instructions.
T237	6106-6191	Sentence	denotes	Beads were resuspended in 2X SDS-PAGE sample buffer and heated for 5 minutes at 95°C.
T238	6192-6307	Sentence	denotes	Material eluted from the beads was fractionated by SDS-PAGE and transferred to PVDF membranes for Western blotting.
T239	6308-6487	Sentence	denotes	In some experiments, cells were cultured for 6 hrs with 25 µM antennapedia-linked SMAC-N7 peptide (Calbiochem) or an appropriate volume of the solvent used for the peptide (DMSO).
T240	6488-6613	Sentence	denotes	After incubation, cells (in peptide- or DMSO-containing medium) were stimulated with antibody-coated beads as outlined above.

bionlp-st-ge-2016-uniprot

Id	Subject	Object	Predicate	Lexical cue
T12037	56-59	P21580	denotes	A20
T12038	234-239	P29965	denotes	CD154
T12273	340-344	P25942	denotes	CD40
T12274	540-544	P33681	denotes	CD80
T12275	607-611	Q96EP0	denotes	HOIP
T12819	930-934	Q96EP0	denotes	HOIP
T12820	1053-1058	Q96EP0	denotes	Rnf31
T12821	1142-1145	P21580	denotes	A20
T12822	1567-1570	P21580	denotes	A20
T12823	2060-2065	Q96EP0	denotes	Rnf31
T12824	2150-2153	P06956	denotes	Cre
T12825	2284-2289	Q96EP0	denotes	Rnf31
T12826	2365-2369	Q96EP0	denotes	HOIP
T13283	2509-2513	Q96EP0	denotes	HOIP
T13284	2587-2590	Q9U6Y4	denotes	GFP
T13285	2713-2717	Q96EP0	denotes	HOIP
T13286	2979-2983	Q96EP0	denotes	HOIP
T13287	3116-3119	P21580	denotes	A20
T13288	3126-3130	Q96EP0	denotes	HOIP
T13500	3452-3456	P33681	denotes	CD80
T13501	3572-3576	P25942	denotes	CD40
T13502	3662-3666	P33681	denotes	CD80
T13790	3858-3861	Q28322	denotes	GLε
T13791	3883-3887	P25942	denotes	CD40
T13792	3912-3915	Q28322	denotes	GLε
T13793	4030-4034	P25942	denotes	CD40
T13794	4118-4122	P05112	denotes	IL-4
T13795	4265-4268	Q28322	denotes	GLε
T13796	4421-4424	Q28322	denotes	GLε
T14219	4534-4538	P25942	denotes	CD40
T14220	4610-4615	P29965	denotes	CD154
T14221	4617-4621	P25942	denotes	CD40
T14222	4617-4628	P29965	denotes	CD40 ligand
T14705	5450-5454	P25942	denotes	CD40
T14706	5551-5554	P21580	denotes	A20
T14707	5735-5739	P25942	denotes	CD40

test2

Id	Subject	Object	Predicate	Lexical cue	Speculation
T12886	2509-2513	Protein	denotes	HOIP
T12887	2518-2522	Protein	denotes	IKKγ
T12888	2713-2717	Protein	denotes	HOIP
T12889	2808-2812	Protein	denotes	IKKγ
T12890	2979-2983	Protein	denotes	HOIP
T12891	2993-2997	Protein	denotes	IKKγ
T12892	3126-3130	Protein	denotes	HOIP
T12893	3131-3140	Negative_regulation	denotes	deficient
T13339	3452-3456	Protein	denotes	CD80
T13340	3457-3469	Positive_regulation	denotes	upregulation
T13538	3858-3861	Protein	denotes	GLε	true
T13539	3883-3887	Protein	denotes	CD40
T13540	3898-3908	Positive_regulation	denotes	activation
T13541	3912-3915	Protein	denotes	GLε
T13542	3916-3929	Transcription	denotes	transcription
T13543	4118-4122	Protein	denotes	IL-4
T13544	4265-4268	Protein	denotes	GLε
T13545	4273-4278	Protein	denotes	Hprt1
T13546	4407-4417	Gene_expression	denotes	Expression
T13547	4421-4424	Protein	denotes	GLε
T13548	4462-4472	Gene_expression	denotes	expression
T13549	4476-4481	Protein	denotes	Hprt1
T13860	4534-4538	Protein	denotes	CD40
T13861	4593-4603	Gene_expression	denotes	expressing
T13862	4610-4615	Protein	denotes	CD154
T13863	4617-4628	Protein	denotes	CD40 ligand
T14273	5450-5454	Protein	denotes	CD40
T11916	223-233	Gene_expression	denotes	expressing
T11917	234-239	Protein	denotes	CD154
T12303	930-934	Protein	denotes	HOIP
T12304	935-944	Negative_regulation	denotes	deficient
T12305	1053-1058	Protein	denotes	Rnf31	true
T12306	2060-2065	Protein	denotes	Rnf31
T12307	2122-2132	Gene_expression	denotes	expression
T12308	2254-2261	Negative_regulation	denotes	disrupt
T12309	2284-2289	Protein	denotes	Rnf31
T12310	2365-2369	Protein	denotes	HOIP
T12311	2378-2388	Gene_expression	denotes	expression
R10095	T12892	T12893	themeOf	HOIP,deficient
R10478	T13339	T13340	themeOf	CD80,upregulation
R10642	T13539	T13540	causeOf	CD40,activation
R10643	T13541	T13542	themeOf	GLε,transcription
R10644	T13542	T13540	themeOf	transcription,activation
R10645	T13547	T13546	themeOf	GLε,Expression
R10646	T13549	T13548	themeOf	Hprt1,expression
R10896	T13862	T13861	themeOf	CD154,expressing
R10897	T13863	T13862	equivalentTo	CD40 ligand,CD154
R9580	T12303	T12304	themeOf	HOIP,deficient
R9581	T12310	T12311	themeOf	HOIP,expression
R9244	T11917	T11916	themeOf	CD154,expressing

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