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{"project":"2_test","denotations":[{"id":"14737183-8125260-84902979","span":{"begin":464,"end":468},"obj":"8125260"},{"id":"14737183-8202545-84902980","span":{"begin":486,"end":490},"obj":"8202545"},{"id":"14737183-8125260-84902981","span":{"begin":592,"end":596},"obj":"8125260"},{"id":"14737183-8202545-84902982","span":{"begin":614,"end":618},"obj":"8202545"},{"id":"14737183-7588057-84902983","span":{"begin":981,"end":985},"obj":"7588057"},{"id":"T65157","span":{"begin":464,"end":468},"obj":"8125260"},{"id":"T83926","span":{"begin":486,"end":490},"obj":"8202545"},{"id":"T93053","span":{"begin":592,"end":596},"obj":"8125260"},{"id":"T24922","span":{"begin":614,"end":618},"obj":"8202545"},{"id":"T34242","span":{"begin":981,"end":985},"obj":"7588057"}],"text":"In laboratory mice, previous studies from our group and others identified two predominant Agouti mRNA isoforms that differ by virtue of their transcriptional initiation site and 5′ untranslated exons. A “hair cycle-specific” transcript is expressed in both dorsal and ventral skin for 2–3 days during early hair growth, while a “ventral-specific” transcript is expressed throughout the entire period of active hair growth, but only in ventral skin (Bultman et al. 1994; Vrieling et al. 1994). Animals carrying the at allele express only the ventral-specific Agouti transcript (Bultman et al. 1994; Vrieling et al. 1994) and have black dorsal hairs and cream-colored to yellow ventral hairs, with a sharp boundary at the level of the limb–body wall articulations and in the middle of the whisker pad. Ventral-specific Agouti isoforms are also expressed in developing skin from embryonic day 10.5 (E10.5) and beyond and may play a role in pigment cell differentiation (Millar et al. 1995). Thus, regulatory elements for ventral-specific Agouti isoforms are responsive to dorsoventral positional cues established in the embryo and whose effects persist after birth."}

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